LOCUS       DAJTOW010000001       136740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTOW010000001 DAJTOW010000000
VERSION     DAJTOW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.001"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO244_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO244_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5661995.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO244_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO244_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5661996.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO244_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO244_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO244_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO244_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5661997.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO244_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO244_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5661998.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO244_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO244_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5661999.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO244_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO244_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5662000.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO244_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO244_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5662001.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO244_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO244_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5662002.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO244_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO244_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5662003.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO244_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO244_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5662004.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO244_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO244_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5662005.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO244_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO244_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5662006.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO244_000014"
     CDS             18619..18894
                     /locus_tag="OO244_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662007.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO244_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO244_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5662008.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO244_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO244_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5662009.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO244_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO244_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5662010.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO244_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO244_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5662011.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO244_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO244_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO244_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO244_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5662012.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO244_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OO244_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO244_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO244_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5662013.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO244_000023"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO244_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5662014.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO244_000024"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO244_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO244_000025"
     CDS             complement(30355..31602)
                     /locus_tag="OO244_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5662015.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKILLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO244_000026"
     CDS             complement(31624..31950)
                     /locus_tag="OO244_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5662016.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO244_000027"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO244_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5662017.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO244_000028"
     CDS             33338..34594
                     /locus_tag="OO244_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5662018.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO244_000029"
     CDS             34709..35035
                     /locus_tag="OO244_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5662019.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO244_000030"
     CDS             complement(35176..36414)
                     /locus_tag="OO244_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5662020.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO244_000031"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO244_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5662021.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO244_000032"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO244_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO244_000033"
     tRNA            complement(40322..40397)
                     /locus_tag="OO244_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="OO244_000034"
     tRNA            complement(40437..40512)
                     /locus_tag="OO244_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="OO244_000035"
     CDS             40733..41092
                     /locus_tag="OO244_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5662022.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="OO244_000036"
     CDS             41094..41486
                     /locus_tag="OO244_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5662023.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO244_000037"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO244_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5662024.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="OO244_000038"
     tRNA            43212..43287
                     /locus_tag="OO244_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="OO244_000039"
     tRNA            43332..43407
                     /locus_tag="OO244_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="OO244_000040"
     tRNA            43412..43487
                     /locus_tag="OO244_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Lys,seq:ttt)
     gene            43518..43643
                     /locus_tag="OO244_000041"
     ncRNA           43518..43643
                     /ncRNA_class="other"
                     /locus_tag="OO244_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43763..44104
                     /locus_tag="OO244_000042"
     CDS             43763..44104
                     /locus_tag="OO244_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5662025.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44095..45021)
                     /locus_tag="OO244_000043"
     CDS             complement(44095..45021)
                     /locus_tag="OO244_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5662026.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGRQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45111..46109
                     /locus_tag="OO244_000044"
     CDS             45111..46109
                     /locus_tag="OO244_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5662027.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46106..46324)
                     /locus_tag="OO244_000045"
     CDS             complement(46106..46324)
                     /locus_tag="OO244_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5662028.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="OO244_000046"
     CDS             complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="OO244_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5662029.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="OO244_000047"
     CDS             complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="OO244_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5662030.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49628..50389
                     /gene="cysZ"
                     /locus_tag="OO244_000048"
     CDS             49628..50389
                     /gene="cysZ"
                     /locus_tag="OO244_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5662031.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50574..51545
                     /gene="cysK"
                     /locus_tag="OO244_000049"
     CDS             50574..51545
                     /gene="cysK"
                     /locus_tag="OO244_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5662032.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51929..52186
                     /gene="ptsH"
                     /locus_tag="OO244_000050"
     CDS             51929..52186
                     /gene="ptsH"
                     /locus_tag="OO244_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5662033.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52231..53958
                     /gene="ptsI"
                     /locus_tag="OO244_000051"
     CDS             52231..53958
                     /gene="ptsI"
                     /locus_tag="OO244_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5662034.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53999..54508
                     /gene="crr"
                     /locus_tag="OO244_000052"
     CDS             53999..54508
                     /gene="crr"
                     /locus_tag="OO244_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5662035.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="OO244_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="OO244_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5662036.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55506..55880
                     /locus_tag="OO244_000054"
     CDS             55506..55880
                     /locus_tag="OO244_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5662037.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55913..56636
                     /locus_tag="OO244_000055"
                     /pseudo
     CDS             55913..56636
                     /locus_tag="OO244_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="OO244_000056"
     CDS             complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="OO244_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5662038.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="OO244_000057"
     CDS             complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="OO244_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5662039.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="OO244_000058"
     CDS             complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="OO244_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5662040.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="OO244_000059"
     CDS             complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="OO244_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5662041.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="OO244_000060"
     CDS             complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="OO244_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5662042.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="OO244_000061"
     CDS             complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="OO244_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5662043.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62978..63778)
                     /gene="murR"
                     /locus_tag="OO244_000062"
     CDS             complement(62978..63778)
                     /gene="murR"
                     /locus_tag="OO244_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5662044.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63942..64838
                     /gene="murQ"
                     /locus_tag="OO244_000063"
     CDS             63942..64838
                     /gene="murQ"
                     /locus_tag="OO244_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5662045.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64842..66266
                     /gene="murP"
                     /locus_tag="OO244_000064"
     CDS             64842..66266
                     /gene="murP"
                     /locus_tag="OO244_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5662046.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="OO244_000065"
     CDS             complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="OO244_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5662047.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67441..68016)
                     /locus_tag="OO244_000066"
     CDS             complement(67441..68016)
                     /locus_tag="OO244_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5662048.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68077..68526)
                     /locus_tag="OO244_000067"
     CDS             complement(68077..68526)
                     /locus_tag="OO244_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5662049.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68513..68938)
                     /locus_tag="OO244_000068"
     CDS             complement(68513..68938)
                     /locus_tag="OO244_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5662050.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69152..70021
                     /gene="amiA"
                     /locus_tag="OO244_000069"
     CDS             69152..70021
                     /gene="amiA"
                     /locus_tag="OO244_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5662051.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70025..70924
                     /gene="hemF"
                     /locus_tag="OO244_000070"
     CDS             70025..70924
                     /gene="hemF"
                     /locus_tag="OO244_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5662052.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="OO244_000071"
                     /pseudo
     CDS             complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="OO244_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="OO244_000072"
     CDS             complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="OO244_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5662053.1"
                     /translation="MINALGLLEVDGMVAAIDAADAVLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="OO244_000073"
     CDS             complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="OO244_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5662054.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74382..75173
                     /locus_tag="OO244_000074"
     CDS             74382..75173
                     /locus_tag="OO244_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662055.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75893..76288
                     /locus_tag="OO244_000075"
     CDS             75893..76288
                     /locus_tag="OO244_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5662056.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76306..76569
                     /locus_tag="OO244_000076"
     CDS             76306..76569
                     /locus_tag="OO244_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662057.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RSLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76592..77134
                     /locus_tag="OO244_000077"
     CDS             76592..77134
                     /locus_tag="OO244_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662058.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77273..77560
                     /locus_tag="OO244_000078"
     CDS             77273..77560
                     /locus_tag="OO244_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662059.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77599..77805
                     /locus_tag="OO244_000079"
     CDS             77599..77805
                     /locus_tag="OO244_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662060.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78117..78602
                     /locus_tag="OO244_000080"
     CDS             78117..78602
                     /locus_tag="OO244_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5662061.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQIWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78583..78861
                     /locus_tag="OO244_000081"
     CDS             78583..78861
                     /locus_tag="OO244_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662062.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78854..80314
                     /locus_tag="OO244_000082"
     CDS             78854..80314
                     /locus_tag="OO244_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5662063.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80348..81157
                     /gene="murI"
                     /locus_tag="OO244_000083"
     CDS             80348..81157
                     /gene="murI"
                     /locus_tag="OO244_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5662064.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81276..81970)
                     /locus_tag="OO244_000084"
     CDS             complement(join(81276..81724,81724..81970))
                     /locus_tag="OO244_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5662065.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="OO244_000085"
     CDS             complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="OO244_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5662066.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="OO244_000086"
     CDS             complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="OO244_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5662067.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="OO244_000087"
     CDS             complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="OO244_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5662068.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84957..87236)
                     /gene="maeB"
                     /locus_tag="OO244_000088"
     CDS             complement(84957..87236)
                     /gene="maeB"
                     /locus_tag="OO244_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5662069.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87525..88475
                     /gene="tal"
                     /locus_tag="OO244_000089"
     CDS             87525..88475
                     /gene="tal"
                     /locus_tag="OO244_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5662070.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88495..90498
                     /gene="tkt"
                     /locus_tag="OO244_000090"
     CDS             88495..90498
                     /gene="tkt"
                     /locus_tag="OO244_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5662071.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90601..91644)
                     /locus_tag="OO244_000091"
     CDS             complement(90601..91644)
                     /locus_tag="OO244_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5662072.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91770..92345)
                     /gene="nudK"
                     /locus_tag="OO244_000092"
     CDS             complement(91770..92345)
                     /gene="nudK"
                     /locus_tag="OO244_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5662073.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92413..94392)
                     /gene="aegA"
                     /locus_tag="OO244_000093"
     CDS             complement(92413..94392)
                     /gene="aegA"
                     /locus_tag="OO244_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5662074.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94598..96315
                     /gene="narQ"
                     /locus_tag="OO244_000094"
                     /pseudo
     CDS             94598..96315
                     /gene="narQ"
                     /locus_tag="OO244_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96517..99630
                     /gene="acrD"
                     /locus_tag="OO244_000095"
     CDS             96517..99630
                     /gene="acrD"
                     /locus_tag="OO244_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5662075.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99729..99788)
                     /gene="ypfM"
                     /locus_tag="OO244_000096"
     CDS             complement(99729..99788)
                     /gene="ypfM"
                     /locus_tag="OO244_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5662076.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100169..100525
                     /locus_tag="OO244_000097"
     CDS             100169..100525
                     /locus_tag="OO244_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5662077.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100529..101656
                     /gene="dapE"
                     /locus_tag="OO244_000098"
     CDS             100529..101656
                     /gene="dapE"
                     /locus_tag="OO244_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5662078.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101684..101884
                     /locus_tag="OO244_000099"
     CDS             101684..101884
                     /locus_tag="OO244_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5662079.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101994..102692)
                     /gene="ypfH"
                     /locus_tag="OO244_000100"
     CDS             complement(101994..102692)
                     /gene="ypfH"
                     /locus_tag="OO244_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5662080.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102766..104781)
                     /gene="tmcA"
                     /locus_tag="OO244_000101"
     CDS             complement(102766..104781)
                     /gene="tmcA"
                     /locus_tag="OO244_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5662081.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104796..105659)
                     /locus_tag="OO244_000102"
     CDS             complement(104796..105659)
                     /locus_tag="OO244_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5662082.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105827..106540)
                     /gene="purC"
                     /locus_tag="OO244_000103"
     CDS             complement(105827..106540)
                     /gene="purC"
                     /locus_tag="OO244_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5662083.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106753..107787)
                     /gene="bamC"
                     /locus_tag="OO244_000104"
     CDS             complement(106753..107787)
                     /gene="bamC"
                     /locus_tag="OO244_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5662084.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107804..108682)
                     /gene="dapA"
                     /locus_tag="OO244_000105"
     CDS             complement(107804..108682)
                     /gene="dapA"
                     /locus_tag="OO244_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5662085.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108828..109400
                     /locus_tag="OO244_000106"
     CDS             108828..109400
                     /locus_tag="OO244_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5662086.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109400..109870
                     /gene="bcp"
                     /locus_tag="OO244_000107"
     CDS             109400..109870
                     /gene="bcp"
                     /locus_tag="OO244_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5662087.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110123..110740
                     /gene="hyfA"
                     /locus_tag="OO244_000108"
     CDS             110123..110740
                     /gene="hyfA"
                     /locus_tag="OO244_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5662088.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDQCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110740..112757
                     /gene="hyfB"
                     /locus_tag="OO244_000109"
                     /pseudo
     CDS             110740..112757
                     /gene="hyfB"
                     /locus_tag="OO244_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112768..113715
                     /gene="hyfC"
                     /locus_tag="OO244_000110"
     CDS             112768..113715
                     /gene="hyfC"
                     /locus_tag="OO244_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5662089.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113732..115171
                     /gene="hyfD"
                     /locus_tag="OO244_000111"
     CDS             113732..115171
                     /gene="hyfD"
                     /locus_tag="OO244_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5662090.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRMSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115183..115833
                     /gene="hyfE"
                     /locus_tag="OO244_000112"
     CDS             115183..115833
                     /gene="hyfE"
                     /locus_tag="OO244_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5662091.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115838..117424
                     /gene="hyfF"
                     /locus_tag="OO244_000113"
     CDS             115838..117424
                     /gene="hyfF"
                     /locus_tag="OO244_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5662092.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117414..119128
                     /gene="hyfG"
                     /locus_tag="OO244_000114"
                     /pseudo
     CDS             117414..119128
                     /gene="hyfG"
                     /locus_tag="OO244_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119138..119683
                     /gene="hyfH"
                     /locus_tag="OO244_000115"
     CDS             119138..119683
                     /gene="hyfH"
                     /locus_tag="OO244_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5662093.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119680..120438
                     /gene="hyfI"
                     /locus_tag="OO244_000116"
     CDS             119680..120438
                     /gene="hyfI"
                     /locus_tag="OO244_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5662094.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120431..120844
                     /gene="hyfJ"
                     /locus_tag="OO244_000117"
     CDS             120431..120844
                     /gene="hyfJ"
                     /locus_tag="OO244_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5662095.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120874..122703
                     /gene="hyfR"
                     /locus_tag="OO244_000118"
     CDS             120874..122703
                     /gene="hyfR"
                     /locus_tag="OO244_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5662096.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122704..122886
                     /locus_tag="OO244_000119"
     CDS             122704..122886
                     /locus_tag="OO244_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5662097.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122908..123780
                     /locus_tag="OO244_000120"
                     /pseudo
     CDS             122908..123780
                     /locus_tag="OO244_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123818..124879)
                     /locus_tag="OO244_000121"
     CDS             complement(123818..124879)
                     /locus_tag="OO244_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5662098.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125092..126555
                     /gene="bepA"
                     /locus_tag="OO244_000122"
     CDS             125092..126555
                     /gene="bepA"
                     /locus_tag="OO244_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5662099.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            126576..126935
                     /gene="arsC"
                     /locus_tag="OO244_000123"
     CDS             126576..126935
                     /gene="arsC"
                     /locus_tag="OO244_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5662100.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127068..127814)
                     /gene="hda"
                     /locus_tag="OO244_000124"
     CDS             complement(127068..127814)
                     /gene="hda"
                     /locus_tag="OO244_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5662101.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127864..129153)
                     /gene="uraA"
                     /locus_tag="OO244_000125"
     CDS             complement(127864..129153)
                     /gene="uraA"
                     /locus_tag="OO244_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5662102.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129239..129865)
                     /gene="upp"
                     /locus_tag="OO244_000126"
     CDS             complement(129239..129865)
                     /gene="upp"
                     /locus_tag="OO244_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5662103.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130189..131226
                     /gene="purM"
                     /locus_tag="OO244_000127"
     CDS             130189..131226
                     /gene="purM"
                     /locus_tag="OO244_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5662104.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131226..131864
                     /gene="purN"
                     /locus_tag="OO244_000128"
     CDS             131226..131864
                     /gene="purN"
                     /locus_tag="OO244_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5662105.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            132036..134102
                     /gene="ppk1"
                     /locus_tag="OO244_000129"
     CDS             132036..134102
                     /gene="ppk1"
                     /locus_tag="OO244_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5662106.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134107..135648
                     /gene="ppx"
                     /locus_tag="OO244_000130"
     CDS             134107..135648
                     /gene="ppx"
                     /locus_tag="OO244_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5662107.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135687..>136740)
                     /gene="pdeF"
                     /locus_tag="OO244_000131"
     CDS             complement(135687..>136740)
                     /gene="pdeF"
                     /locus_tag="OO244_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5662108.1"
                     /translation="YQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQI
                     GVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNR
                     LQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPGSFLPVAHEFGLSSSI
                     DMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIF
                     EVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFI
                     RNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQ
                     PLIDTLNEIEPIRESA"
BASE COUNT        34138 a        35176 c        33595 g        33831 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccacctg aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgagggaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtaccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaacaggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    43501 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
    43561 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
    43621 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
    43681 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
    43741 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
    43801 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
    43861 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
    43921 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
    43981 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
    44041 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
    44101 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
    44161 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    44221 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
    44281 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    44341 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
    44401 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    44461 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    44521 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    44581 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    44641 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    44701 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    44761 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    44821 agccaactgc cgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    44881 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
    44941 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    45001 ttgttttaaa gaataattca tctgttttct tcatgaatga atgcaataaa tcaattttat
    45061 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    45121 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
    45181 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
    45241 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    45301 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
    45361 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    45421 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    45481 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    45541 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    45601 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
    45661 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    45721 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    45781 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    45841 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    45901 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    45961 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    46021 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    46081 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    46141 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    46201 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    46261 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    46321 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    46381 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    46441 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    46501 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    46561 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    46621 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    46681 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    46741 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    46801 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    46861 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    46921 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    46981 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    47041 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    47101 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    47161 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    47221 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    47281 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    47341 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    47401 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    47461 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    47521 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    47581 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    47641 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    47701 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    47761 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    47821 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    47881 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    47941 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    48001 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    48061 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    48121 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggttgg cgaatcaggc
    48181 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    48241 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    48301 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    48361 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    48421 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    48481 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    48541 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    48601 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    48661 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    48721 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    48781 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    48841 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    48901 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    48961 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    49021 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    49081 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    49141 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    49201 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    49261 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    49321 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    49381 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    49441 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    49501 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    49561 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    49621 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    49681 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    49741 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    49801 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    49861 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    49921 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    49981 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    50041 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    50101 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    50161 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    50221 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    50281 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    50341 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    50401 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    50461 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    50521 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    50581 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    50641 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    50701 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    50761 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    50821 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    50881 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    50941 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    51001 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    51061 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    51121 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    51181 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    51241 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    51301 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    51361 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    51421 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    51481 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    51541 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    51601 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    51661 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    51721 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    51781 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    51841 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    51901 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    51961 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    52021 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    52081 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    52141 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    52201 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    52261 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    52321 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    52381 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    52441 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    52501 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    52561 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    52621 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    52681 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    52741 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    52801 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    52861 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    52921 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    52981 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    53041 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    53101 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    53161 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    53221 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    53281 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    53341 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    53401 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    53461 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    53521 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    53581 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    53641 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    53701 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    53761 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    53821 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    53881 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    53941 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    54001 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    54061 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    54121 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    54181 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    54241 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    54301 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    54361 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    54421 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    54481 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    54541 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    54601 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    54661 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    54721 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    54781 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    54841 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    54901 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    54961 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    55021 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    55081 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    55141 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    55201 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    55261 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    55321 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    55381 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    55441 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    55501 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    55561 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    55621 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    55681 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    55741 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    55801 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    55861 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    55921 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    55981 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    56041 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    56101 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    56161 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    56221 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    56281 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    56341 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    56401 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    56461 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    56521 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    56581 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    56641 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    56701 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    56761 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    56821 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    56881 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    56941 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    57001 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    57061 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    57121 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    57181 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    57241 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    57301 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    57361 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    57421 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    57481 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    57541 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    57601 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    57661 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    57721 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    57781 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    57841 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    57901 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    57961 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    58021 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    58081 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    58141 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    58201 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    58261 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    58321 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    58381 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    58441 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    58501 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    58561 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    58621 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    58681 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    58741 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    58801 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    58861 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    58921 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    58981 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    59041 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    59101 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    59161 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    59221 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    59281 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    59341 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    59401 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    59461 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    59521 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    59581 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    59641 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    59701 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    59761 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    59821 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    59881 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    59941 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    60001 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    60061 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    60121 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    60181 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    60241 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    60301 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    60361 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    60421 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    60481 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    60541 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    60601 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    60661 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    60721 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    60781 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    60841 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    60901 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    60961 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    61021 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    61081 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    61141 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    61201 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    61261 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    61321 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    61381 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    61441 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    61501 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    61561 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    61621 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    61681 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    61741 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    61801 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    61861 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    61921 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    61981 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    62041 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    62101 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    62161 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    62221 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    62281 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    62341 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    62401 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    62461 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    62521 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    62581 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    62641 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    62701 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    62761 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    62821 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    62881 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    62941 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    63001 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    63061 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    63121 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    63181 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    63241 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    63301 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    63361 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    63421 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    63481 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    63541 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    63601 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    63661 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    63721 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    63781 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    63841 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    63901 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    63961 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    64021 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    64081 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    64141 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    64201 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    64261 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    64321 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    64381 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    64441 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    64501 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    64561 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    64621 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    64681 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    64741 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    64801 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    64861 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    64921 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    64981 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    65041 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    65101 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    65161 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    65221 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    65281 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    65341 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    65401 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    65461 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    65521 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    65581 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    65641 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    65701 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    65761 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    65821 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    65881 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    65941 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    66001 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    66061 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    66121 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    66181 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    66241 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    66301 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    66361 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    66421 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    66481 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    66541 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    66601 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    66661 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    66721 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    66781 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    66841 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    66901 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    66961 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    67021 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    67081 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    67141 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    67201 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    67261 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    67321 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    67381 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    67441 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    67501 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    67561 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    67621 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    67681 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    67741 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    67801 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    67861 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    67921 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    67981 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    68041 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    68101 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    68161 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    68221 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    68281 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    68341 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    68401 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    68461 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    68521 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    68581 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    68641 agccccgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    68701 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    68761 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    68821 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    68881 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    68941 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    69001 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    69061 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    69121 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    69181 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    69241 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    69301 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    69361 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    69421 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    69481 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    69541 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    69601 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    69661 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    69721 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    69781 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    69841 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    69901 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    69961 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    70021 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    70081 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    70141 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    70201 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    70261 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    70321 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    70381 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    70441 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    70501 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    70561 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    70621 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    70681 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    70741 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    70801 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    70861 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    70921 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    70981 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    71041 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    71101 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    71161 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    71221 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    71281 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    71341 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    71401 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    71461 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    71521 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    71581 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    71641 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    71701 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    71761 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    71821 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    71881 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    71941 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    72001 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    72061 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    72121 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    72181 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    72241 ttgcttcggc tggcggttaa agccagtaac cagccactgg gtgtcatcgt ctggccgacc
    72301 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    72361 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    72421 cacttcgtga ctgagcagac gaacgttagc tgctttgagc acggcatccg ctgcatctat
    72481 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    72541 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    72601 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    72661 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    72721 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    72781 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    72841 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    72901 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    72961 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    73021 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    73081 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    73141 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    73201 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    73261 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    73321 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    73381 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    73441 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    73501 actagccgca aaaatgcggc cctatttgcc agaacctttt attattcaat tggttagtgc
    73561 aagaaaaagc gtgattgctg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    73621 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    73681 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    73741 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    73801 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    73861 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    73921 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    73981 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    74041 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    74101 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    74161 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    74221 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    74281 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    74341 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    74401 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    74461 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcag aaaagagata
    74521 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    74581 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    74641 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    74701 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    74761 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    74821 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    74881 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    74941 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    75001 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    75061 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    75121 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    75181 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    75241 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    75301 gatatcaatt taattaattc tgatgtggtg atttgcagga aaaaaaaacc cgccggagaa
    75361 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    75421 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    75481 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    75541 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    75601 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    75661 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    75721 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    75781 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    75841 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    75901 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    75961 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    76021 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    76081 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    76141 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    76201 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    76261 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    76321 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    76381 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    76441 tcacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    76501 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    76561 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    76621 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    76681 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    76741 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    76801 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    76861 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    76921 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    76981 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    77041 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    77101 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    77161 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    77221 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    77281 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    77341 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    77401 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    77461 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    77521 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    77581 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    77641 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    77701 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    77761 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    77821 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    77881 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    77941 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    78001 tgatatgttt atggttttat attaattaaa atatatctaa atttgatagt tttaaaattg
    78061 caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga
    78121 aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc
    78181 tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat
    78241 tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt
    78301 tctgtcaaat atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa
    78361 acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta
    78421 tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg
    78481 gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac
    78541 gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat
    78601 aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg
    78661 ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg tgtcgttacg
    78721 actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt
    78781 aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt taaaaatgtt
    78841 cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg
    78901 gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc
    78961 agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc
    79021 tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg
    79081 atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct
    79141 acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat
    79201 tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt
    79261 cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc
    79321 gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat
    79381 tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg
    79441 tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct
    79501 tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa
    79561 aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag
    79621 tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt
    79681 ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc
    79741 atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac
    79801 aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg
    79861 tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt
    79921 tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt
    79981 tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc
    80041 tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg
    80101 attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac
    80161 tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat ttttattaat
    80221 tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga
    80281 accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag
    80341 gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg tcggtggatt
    80401 gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga
    80461 taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa
    80521 aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat
    80581 atcctcaact ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat
    80641 cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg
    80701 tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt
    80761 gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga
    80821 aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat
    80881 ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa
    80941 tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat
    81001 ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt
    81061 gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat
    81121 tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtac accgtaaaat
    81181 cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg
    81241 gacaaaccca gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata
    81301 aacgctccga tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga
    81361 cccaaccgct taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
    81421 attttgccgg tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg
    81481 ctggtcagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
    81541 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    81601 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    81661 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    81721 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    81781 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    81841 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    81901 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    81961 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    82021 catcacccat ccagcattat aaaacatact aacaataact aaattataat aatcacaact
    82081 tacaatcaaa taataaatgc ataagattaa caatgatttt ttcgggttat atttatctaa
    82141 aaaagcagaa tattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac
    82201 taacaaactt gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg
    82261 tttttatttt tcattttctc ttttcttctc tttccggttc tgttctggtt ttggtttttt
    82321 cctttttatt tttattcttt cttcagggtt atgctaaaac accaaaacat ttttcaccac
    82381 aaaagcatcc aatcatcttg ccagaccaat caccttaacc ggatacactg ccggatataa
    82441 tcccactaac acccagacac caagcaccat acctgataac taccgtattc aggaaggaaa
    82501 tgcgagtgat ttcaccgtca ccggcacaac cgatccgcca aaaagaggcg taccaatgtc
    82561 gatatagtcc cccgcgcgga caatcacttc gtcaatgact gccaacggga gttgttgtag
    82621 ctgtgggcgc aacaacatgc ccagagcttt accaaagtcc tgcccggcca ccaccagcag
    82681 gggatgcgga ttcggaaaac gcgcgacgaa atcgaccagc gcgttgatga ccgtcagaac
    82741 cgcggcgtaa cgcacaggca gcgaggcggg aagcgccagc acgtacacgt cagttttggg
    82801 atcaagatcc agctgaagca gcgcctgttg ccaggcactc accagatccg tttcatcaat
    82861 cgggatcgcc accggcaaat tgcgcagcgg cagttgtacg ccctccagcc agattgtgct
    82921 gccagagagc gaaagggtat gtgcacccgc gccaatcacc gtggagcgta cggtttgcgc
    82981 cggaaactgc acattcatct cacgcaggcg cggatggtca tgcagcgccg ttgccagaag
    83041 tgggccaata tcggcaaaac agaacgggtt ggcgggctgg tggcgataac attcgcccac
    83101 gcctccagaa agcgtaatga tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat
    83161 caatgcctgc gcgagcggcg aaagcgttcc gtcaatcact tcgacaatca gcgccgccat
    83221 ccgccgggta acctgcacca gctgcgcgcc ggtcagcgaa cgggcgtcag tgcctgcacc
    83281 gaagcactca tccacaatca tctgccccgg tttatgagcg taaaccacgc gcccctggct
    83341 gtcggtttcc agcaggcgac caccgacgtt gagacaggca gtgccgctga tttttccggc
    83401 atcgaacagg gcgtagttcg cggtgccacc gccgatatcg atattcagta cccgacacag
    83461 ccgttgttca gaaagggttt gcgccccggc tccgtgaccg gcgatcacgg attcgaggtg
    83521 cggcccggcg ctggcgacga caaaatcgcc cagcgactgt ttttccttca tcactttttc
    83581 catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg caatgatggt
    83641 ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca cggacgccgt
    83701 taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg ccacttcacg
    83761 ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc
    83821 gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg
    83881 tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct
    83941 cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag
    84001 catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg ccaatatcca
    84061 gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag atcagcatgt
    84121 ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac
    84181 ccggcgtatc aatatcgccc ttatcattaa attccacggc cagtgttttt ctggcgaggg
    84241 tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg ctacccacaa
    84301 acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat
    84361 ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact gcgcccaccg
    84421 agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc acatcagcgg
    84481 ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc
    84541 caattgcgcc cgcatccgga acgccgattt tcttcgccag ttcctcaccg ggatgcgcga
    84601 tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat
    84661 ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg tcagagtaaa
    84721 taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat caataagaat ggagagatat
    84781 ttactgttat gaataaactt tcatatgcga cgcatgaata taaataaaat aatatcgaga
    84841 tattttatat actgattttt ttattattcg caaactcagt aataagcttt gctaatatat
    84901 ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaag aatgaattac
    84961 agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat acgacgcacc
    85021 gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca
    85081 gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc agcttccatg
    85141 ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat acggtcgttg
    85201 cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagatctggc
    85261 gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa
    85321 gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa acgacggaca
    85381 gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc gtcattgacg
    85441 taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcattcat tgcacctgcg
    85501 gtgtgaacgc catcgcgata accaaagaca tttttcacca cactaaaatg ttcatgataa
    85561 tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat gatcgcgccg
    85621 atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt cctgagtgac accgcgacgc
    85681 ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga ttcgttattg
    85741 acgatttcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat gcgcatttcg
    85801 atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac cagttcctga
    85861 gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt cggcgctttg
    85921 cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctcagtcagc
    85981 ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc
    86041 gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg
    86101 atgtattccg gaccaaagct cagatcctga acgccatacg ctgaagccac cacttcactc
    86161 tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg
    86221 atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca aagaacgttg
    86281 ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg acgcacttct
    86341 ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggtgcc
    86401 cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca gcccaggaaa
    86461 atatccgcac cttcaatcac atcatcgagg gtacgtttgc cgtcatccac caccgcatat
    86521 gcggctttgg tttccgccat atttggctca cggccctgat agataacgcc ttttgaatcg
    86581 caaaccacga tgttatgttt ttgcagcccc agcgctacca gcaggttcat acaggcgatt
    86641 gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg
    86701 cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg atcgtcgtgg
    86761 aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct
    86821 ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcggcgac gacttcaata
    86881 aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc agcgaatttc
    86941 ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct
    87001 aaccccagca ccgccgtacc gttagagatc accgcaacca ggttaccgcg cgcggtatat
    87061 ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag
    87121 taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc
    87181 cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg
    87241 ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc tgcccacaac
    87301 gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc
    87361 attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt aatccagcaa
    87421 taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa
    87481 ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg
    87541 gcatcaaaca gttcaccact gtcgtggcag acagctgcga tattgagtcc attcgccatt
    87601 atcatcccca ggatgccacc actaatcctt cgctgttact caaggctgcc ggattatcac
    87661 aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg
    87721 aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaattctca
    87781 aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa
    87841 agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc gttgagaaat
    87901 cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc gaagagctgg
    87961 aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttacccag gcgcgggcct
    88021 gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg gcgtatttat gactggtatc
    88081 aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt aaatcggtgc
    88141 gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct
    88201 tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact atcgcaccga
    88261 atttgctgaa ggagctgcag gaaaaagttt ctccagtggt acgtaaatta atcccgcctt
    88321 ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg gagcacaatc
    88381 aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct gttgatcaac
    88441 gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc
    88501 cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgctgtaca aaaagccaat
    88561 tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat
    88621 tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc
    88681 aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta cgacctgccg
    88741 ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca cccggagatt
    88801 ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt ggcgaacgcc
    88861 gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc agaccatgag
    88921 atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatttcc
    88981 cacgaagtct gttcgctggc aggcacgctg ggactaggca agctgattgg tttttacgat
    89041 cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga tacggcaaaa
    89101 cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacga tccgcaggcg
    89161 gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc
    89221 cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca
    89281 ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca ccatccgcca
    89341 tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gtgaaaaagg cgaaaaagcg
    89401 cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa
    89461 gagtttcccc gacggatgag cggtggttta ccgaaggact gggagaaaac gactcagaaa
    89521 tatatcaatg agttacaggc aaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat
    89581 acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc ggatctggct
    89641 cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc ggcgggcaac
    89701 tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg catcgcgcac
    89761 cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac
    89821 gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac ccacgactca
    89881 attggcctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc cagcctgcgc
    89941 ttaacgccaa atttcagcac ctggcgaccg tgcgatcaga tggaagcggc ggtgggctgg
    90001 aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctcaag gcagaatctg
    90061 gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg
    90121 aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga gatggaaatt
    90181 accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt agtttccctg
    90241 ccctcgaccg atattttcga cgcccaggat gaggaatatc gggagtcggt gttgccttct
    90301 aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta caagtatgtt
    90361 ggtctgaaag gggcaattgt cgggatgacg ggttacgggg aatctgctcc ggcggataag
    90421 ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtgctg
    90481 ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat gttcccctca ccctaaccct
    90541 ctccccagaa gggcgagggg acggtccgtg cgctttttag ggccgcatct gcgattttgt
    90601 ttaacgggtg atccacaacg tgggcctggc gtctggccca tgccagttat cgcaggtagg
    90661 ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat
    90721 gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc
    90781 aaatgttgcg ttcatcagtt ccagttcatt cgtctcctgg ccgttgttga acggcaaacg
    90841 caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg ccaaatcaat
    90901 ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat cggtcagcgc
    90961 agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat tcatccgcca
    91021 gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt
    91081 aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg gttcgtcccc
    91141 tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa
    91201 cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt
    91261 cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa
    91321 ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat ctaatagcag
    91381 ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc gctcaatacg
    91441 taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca gtccattatg
    91501 atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct gccagtcgga
    91561 aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa
    91621 gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac
    91681 aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat
    91741 aataaacaac gaaatattat tgctattttt cagtccatta aatgtgacat ttgcaaatag
    91801 ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc
    91861 tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc accggcgtta
    91921 gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcca
    91981 ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca
    92041 atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt
    92101 tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc
    92161 agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg
    92221 taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaagtaatg
    92281 ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc
    92341 gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag tcgggtttgt
    92401 ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg
    92461 tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc
    92521 gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc
    92581 gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg
    92641 tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt cactgccctc
    92701 aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa
    92761 acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct ggacgttaaa
    92821 ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt
    92881 agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg
    92941 cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt
    93001 gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcaatgag
    93061 gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga
    93121 acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagca aatccaaaga
    93181 gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc
    93241 ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa
    93301 gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac
    93361 atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct tgtccacttt
    93421 ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatatagcg
    93481 ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg caccttcaca
    93541 caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga
    93601 aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggaatatg
    93661 gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa ggcagcgcga
    93721 ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa aaccggtttt
    93781 gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc
    93841 gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg gttgatgctc
    93901 ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga cgtcagtgac
    93961 cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt cgcgccctgc
    94021 acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac
    94081 gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg
    94141 ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc
    94201 gcagggagca tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat
    94261 aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc
    94321 catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat
    94381 aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt
    94441 tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat aagtaactat
    94501 ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc gtttcagcga
    94561 actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc
    94621 ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat
    94681 cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc
    94741 gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa
    94801 tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca acctcaacgt
    94861 ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga
    94921 gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta
    94981 tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct
    95041 gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac
    95101 tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca
    95161 gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc cgaatgagct
    95221 tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat tgtacctttc
    95281 gctggaggcg tcagtagaag aaaagacccg cgatctccac gagaccaagc gtcgtctgga
    95341 ggtgttgtat cagtgcagtg ttcccaggcg ctgaacacca gccagattga tgtgcattgt
    95401 ttccgccata ttttgcagat tgttcgcgac aatgaagcag ctgaatatct ggagctgaac
    95461 gttggggata actggcggat tagcgaaggg aagccaaacc ctgagctgcc aatgcagatt
    95521 ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt
    95581 tcatcatcag aaccgctgct taacagtgtt tcatcgatgc tgggacgcgg tttgtacttt
    95641 aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc
    95701 cgcgaattgc acgactcgct ggctcaggtg ctttcttact tacgtatcca gttgacgtta
    95761 ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc
    95821 caggcgttga atgatgctta tcggcagtta cgcgagctgt tgactacttt ccgcctgacg
    95881 ctgcagcagg cggatctccc ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa
    95941 accagcgcta aactgaccct cgactgccgt ctgccaaccc tggcactgga tgcgcaaatg
    96001 caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac
    96061 gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc
    96121 cgtgataacg ggattggtat cggtgaaccg aaagaacccg aaggtcatta tggactgaat
    96181 atcatgcgcg aacgcgcgga acggctgggc gggacgctga ctttttcaca accttccggc
    96241 ggcggcacgt tagtgagtat tagctttcgc tccgcagagg gtgaggggaa ggggaacacg
    96301 ccggctgagg tgtaaataat taccctcaca ccggccctct ccctttaaag gcgagaggga
    96361 aaaacgtgcc catattgacc tgtaagcgag tgtgtcagca atctcatgat aatttacatt
    96421 aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag caagtcaagc
    96481 ctacaacaat acgcagaaac acgaggtcct cttttaatgg cgaatttctt tattgatcgc
    96541 cccatttttg cctgggtgct gacaatcctg ttgtgtctga caggtaccct ggcgattttt
    96601 tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt gaccgctaac
    96661 tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat cgagcaaaat
    96721 atgaccggcc tcgataatct gatgtatatg tcatctcaga gcagtggcac cggtcaggca
    96781 tctgtcactt taagttttaa agcaggcacc gatccggacg aagccgtgca gcaagtacaa
    96841 aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg
    96901 gtgcgtaaaa ccggcgatac caacattctg accattgcct tcgtctctac cgatggctcg
    96961 atggataaac aggatattgc tgattatgtt gccagtaata ttcaggaccc gttaagccgc
    97021 gtgaatggcg tcggggatat agatgcctat ggttcgcaat attccatgcg tatctggctg
    97081 gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga tgccattgag
    97141 tcacagaacg cgcagattgc ggttgggcaa cttggtggta caccttccgt cgataagcag
    97201 gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa caccagaaca gttccgcgat
    97261 atcaccttgc gggtgaatca ggacggctca gaggtaaggc tgggcgatgt cgccaccgtc
    97321 gaaatggggg cggagaaata cgattatctt agccgcttca atggtaagcc agcctccggg
    97381 ttgggggtaa aactggcctc cagcgcaaac gaaatggcga cagcggagct ggtgctcaat
    97441 cgtctcgacg agctggcgca gtatttcccg cacggactgg aatacaaggt ggcgtatgaa
    97501 accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct ggaagctatc
    97561 gctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac gctgataccc
    97621 actatcgccg tgccggtggt gttgatggga accttctccg tactttacgc cttcggttac
    97681 agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga tcggtctgct ggtggatgac
    97741 gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact cactcctcgc
    97801 gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat tgcgatggtg
    97861 ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc catctatcgc
    97921 cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tactggtagc gatgctcctc
    97981 actccggctc tgtgtgccac gctacttaag ccactgaaaa aaggtgagca tcacgggcaa
    98041 aaaggttttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg ctacgaaaaa
    98101 ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt cctgctgctt
    98161 ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgg
    98221 ggcatgttta ctacctcggt acaattgccc agcggttcaa cccaacaaca gaccctgaaa
    98281 gtcgttgagc aaatcgagaa atattacttc acccatgaaa aagacaacat catgtcggtg
    98341 tttgccaccg ttggttctgg ccctgggggt aacgggcaaa acgtggcgag aatgtttatc
    98401 cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc cattatcgag
    98461 cgtgcaacga aggcgtttaa ccaaattaaa gaagctcgcg ttatcgccag cagcccacca
    98521 gcaattagcg gtcttggtag ttctgcaggt tttgatatgg agttgcagga ccacgctgga
    98581 gcgggtcacg atgcgctgat ggcagcacgt aatcagttgc tggcgctggc ggcggaaaac
    98641 ccggagctaa cccgtgtgcg ccataacggc ctcgatgaca gtccgcagtt gcagattgat
    98701 atcgaccagc gtaaagctca ggcgctgggc gttgatatcg acgatattaa cgacacactg
    98761 caaaccgcct ggggttcgag ctatgtgaat gactttatgg atcgcggtcg cgtgaagaaa
    98821 gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgacatcaa tctctggtat
    98881 gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc acgctgggaa
    98941 acaggctcgc cgcgtctgga acgctataac ggttattctg cggttgagat tgttggggaa
    99001 gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt agtgaagcag
    99061 ctgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg gctttccggc
    99121 gcgcaggctc cggcgctgta cgccatttcc ttgctggtgg tattcctgtg tctggcggca
    99181 ttgtatgaaa gctggtcggt accgttctcg gtaatgctgg tcgtgccgct gggggtaatc
    99241 ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc
    99301 ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac
    99361 gagatgaacc aaaaaggcca tgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt
    99421 ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc aatggcaacc
    99481 agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat gggcgggatg
    99541 atttcagcta ctattctggc tatttacttc gtgccgctgt tctttgtgct ggtgcgccgc
    99601 cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg ccaatgtgtc
    99661 gcctttttca actttccgat taagaacctg ctcagcgggt tcttgctgtt tatactttgt
    99721 ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc cagttcacgt
    99781 tcaatcataa caacctctct tataattatg ggtattctac ggaaacaata taccgtggtg
    99841 aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt atgtaaatac
    99901 tatggtcctg cggtgataat ttgtatgtga tacacagcaa catttcgaga tattcatacg
    99961 gcatctaata ctgatttaat tctggttaaa atacagacag ataacaagat gaatattctt
   100021 aatgtttacg ttaaaaatgt ttaatattat ttaatagttg ttaatttgaa tacttcgata
   100081 atgttatatt ttctgataat catttgcagg caaaatgttt tcacccttaa atgagtattt
   100141 attctcataa atcgaaaaag gattcattat ggttacactt tacggcatca aaaattgtga
   100201 caccattaaa aaggctcgcc gttggctgga agccaataac atcgactatc gttttcatga
   100261 ttaccgcgtc gatgggctgg acagcgaatt attgaacggt tttatcaacg aattaggctg
   100321 ggaagcgtta ctcaacaccc gtggtacaac ctggcgtaaa ctggacgaaa ctacccgcaa
   100381 taaaatcacc gatgcggcct ctgcggcggc attaatgact gaaatgcctg caattatcaa
   100441 acgtccattg ctctgcgcgc ctggtaagcc tatgctgctg ggtttcagtg attccagtta
   100501 tcagcaattt ttccatgagg tgtagtctat gtcgtgcccg gttattgagc tgacacaaca
   100561 gcttattcgc cgcccttccc tgagtcctga tgacgcagga tgtcaggctt tgttgattga
   100621 acgtttgcag gcgatcggtt ttaccgttga acgcatggat tttgccgata cgcagaattt
   100681 ttgggcatgg cgtgggcaag gtgaaacgtt agcctttgcc gggcataccg acgtggtgcc
   100741 gcctggcgac gccgatcgtt ggatcaatcc gccgtttgaa cccaccattc gtgacggcat
   100801 gttattcggg cgcggtgcgg cagatatgaa aggctcgctg gcggcgatgg tggtagcagc
   100861 agaacgtttt gtcgcacaac atcccaacca tacggggcga ctggcatttc tgatcacttc
   100921 tgatgaagaa gccagtgccc acaacggtac ggtaaaagtc gtcgaggcgt taatggcacg
   100981 taatgagcgt ctcgattact gcctggtcgg cgaaccgtcg agtatcgaag tggtaggtga
   101041 tgtggtgaaa aatggtcgtc gcggatcgtt aacctgcaac ctcaccattc atggcgttca
   101101 ggggcatgtt gcctacccac atctggctga caatccggta catcgcgcag cacctttcct
   101161 taatgaatta gtggctattg agtgggatca gggcaatgaa ttcttcccgg cgaccagtat
   101221 gcagattgcc aatattcagg cgggaacggg cagtaacaat gttattccgg gtgaactgtt
   101281 tgtgcagttt aacttccgct tcagcaccga actgactgat gagatgatca aagcgcaggt
   101341 acttgccctg cttgaaaaac atcaactgcg ctatacggtg gattggtggc tttccgggca
   101401 gccatttttg accgcgcgcg gtaaactggt ggatgcggtc gttaacgcgg ttgagcacta
   101461 taatgaaatt aaaccgcagc tactgaccac aggcggaacg tccgacgggc gctttattgc
   101521 tcgcatgggg gcgcaggtgg tggaactcgg gccggtcaat gccactattc ataaaattaa
   101581 tgaatgtgtg aacgctgccg acctgcagct acttgcccgt atgtatcaac gtatcatgga
   101641 acagctcgtc gcgtgatgag tggttctgca agaggaaata agcatggact ggctggctaa
   101701 atattggtgg attctggtga ttgtcttttt ggtaggcgtc ctgctgaacg tgattaaaga
   101761 tctcaagcgc gtcgaccata agaaatttct cgccaacaag ccggagcttc ccccgcatcg
   101821 tgatttcaac gataagtggg acgatgacga cgactggccg aaaaaggatc aaccgaagaa
   101881 gtaagctgag tgctcaaccg taggccggat aaggcgttaa cgccgcatcc ggcaatggtg
   101941 aacgatccct gatgcgacgc tcacgcgtct tatcaggcca ggtaatctgt cttttacatc
   102001 atttcaatca catcatcatc gccaggctta ccgccactta gcgcttggtc gaaataatgt
   102061 ttcggaatgg tataacgcaa gtgatccagg gcaaattgca tactgcgatt gtcgatcgca
   102121 tggcctaaat cctccacgat atccagcgtc acgtcaccac cggcactgat taacgcttcc
   102181 tgcgccgcaa ctgcatgcgc cagatcgata accggatctt caccaccatg aatcaagtga
   102241 attgtcgtgg ctgtagacgc ggtctccggc aggctcgcat agcgtccgtt aaaggcaatg
   102301 acgcgcgaag caaggcccgg ttcggcttta atgctctcca gcgccataat cgcgccttgt
   102361 gagaaaccaa ttagcgccgt ggcgttagcc cctactccgc tctgtttctg ccagtagcgt
   102421 accgtttcaa taaacgtcgg catgatggca tccacacgcg cctggcgatt atcttccgta
   102481 ataccctgca ctgaaaacca ctgacgcccc gccggattac cgctcggctc cgcgccaccg
   102541 acactcacca ccagcgcatc aggaaacagc ggtgcaaacc agctgcctat ttcccccatt
   102601 gccaccgggt tatccccgac accgtggaaa agcagtaaca actgttgtgc aggtttatcc
   102661 gggctttgaa caacaaaatg gtcatgtttc atggcgaact ccttaactga tgtcattaat
   102721 tttacgccgc tgactcacaa tgaccatggg agaaaattga aggagtcagt gaaaaaattg
   102781 ccattgcgtt atgcgatcgc gcagacgctc agtgcgaacc tcatttagtg cgaacagcgc
   102841 ctgcgcggcc tcttcccgct ggcgaaccag taacatcttg cggcctgaaa gtttaagtgt
   102901 ggtacataac tgcgcgtcac tggcgttttt ctgtaaacgc ccacgcagcg ccggtaatgc
   102961 cagttcactg gtttgtagca gacgcaataa gcaacctaac gatgttaata gcggacgatg
   103021 agcgaaagca aaaccggcca gttcaagcca gtcgtcgtca gaaaggatgg catcgtttag
   103081 tggatcaaca ggaagcattt cgccattcca ctgcgcgaga gcttgcgcat cgcgacgtaa
   103141 acggtaatgc tcacgttcag ccagctgttt acccgcatca ctcatcggta acagcgccat
   103201 cgccgtatag caaccgctgc tggcttcccg atgattaccc atccgcacca gcacaaaacc
   103261 gcagcgttgc cagaaacgcc ataactcccc agtgtaacca aaactcaccg aaagatagtc
   103321 gaggtcatgc gtatattgca aagctccagc aataagttgt cgccctgtgc cttcccgctg
   103381 acgagccgga tgaactgcta tccggctaac ccgccttccg cgcaatgtcg ccgccagtgg
   103441 attgctgccg tgcgccgcca gcgactgggc caccagatta ccccgcgggc gacgaaaacc
   103501 tgcccatacc gcctgactga gttcttgaga taatccgccc tcatccacca gccacaacgc
   103561 cccggcaatc tcgttttcgc cagccgcctg taaaaaatgt tgccctggtg catccatcat
   103621 ccggcgtaaa tccagcggcg aggtccggta gtgcgcacca gacaataact gataaacctt
   103681 taacggcgtt tctggctcgc ttcgccataa cgtttgttca aatgcggaaa tgacgatatt
   103741 gccttgtggt gtatgggtga agttttcatc gttaaaaacc agtgcctcac taaccatttt
   103801 ttccagcggg catccctgtg cccagcggat cggctgttgc agttcaaaac ggtgtaaatg
   103861 cggaaagcga gcgcaaaatt tcagcaaaaa accacgtccg gtaccttcgt agccctgcac
   103921 cgtagtggtt aacaacgttc gaggaaaacg cgataccagt tgatgcaaca acggcgcagg
   103981 tatggctgcg gcttcgtcga ccaccagcca gtcggcttgc tcatcgctgg ctaacaaggc
   104041 atccggtgca ataaagcgaa acttctcgcc cgcaaattgt gccagtacat ccgttgccgc
   104101 ttttgcgggc gcggtgacaa tcgcactgcc cgcaatacga gaaatgagtt gccctgccaa
   104161 cgccgactta ccgcgcccac gcgcagccgt taccgctgcc acgcccggcg gcatggtcag
   104221 tagctgctgt aagagttgct gttgttctgg ttgtggtgcg ccggtcgcgg ggtgccagtc
   104281 agtacgggga gtaaaatgcg ccaacgagaa cggctggttt tgccgccaga ggatagcgtc
   104341 gttatccgcc gtaagtacgc gtttgagatg ctggacaaaa tgcggcgtcg caatagggtc
   104401 agggcaatca ctccagcgca gcgagtcggc atcaggttgg ttttcccact cttcccatac
   104461 agggagtaac aaaaccagcc agcttcccgc tttcaacgtt ccgctaagtg ctgcaaaggc
   104521 agcggcatca aagccgtggc gggcgtcgaa taccgcatgc cggaactcgc gcccaagtaa
   104581 agtttgtagc gccgagggtg aacagtggtt ttcagcatct agctgcggcg aaatccacag
   104641 ccagtcgcca ggtaaggcat cacgcaactt aagagcatga tcaaaacacc aaccctcttc
   104701 cccgctcaac accagcaagc ggcagatccc ttcacgtttc atttgcgctg ttaatgtgta
   104761 aagcgcagtc agttcagcca tccctgcccc ggaaattaaa tgcttttacc aaaagtattg
   104821 cattgtgccg gatcgccgct gtcgaaacca cgtttaaacc agctgtagcg ttgctgagaa
   104881 gtgccatgtg tgaaactgtc tggtactact cgcccctgac tttgctgttg taaacggtca
   104941 tcgccgatgg cctgcgccgc attcagcgcc tcttccagat cgccagtttc cagaacgcct
   105001 tgctgctgca tactatgccc ccagacaccg gcaaaacagt cggcctggag ttccatacgc
   105061 acagataagc ggtttacttc cgcctgcgtc gcgttttgtt gcagttgacg aactttcggc
   105121 tcgatgccta acagtttctg cacatgatga ccgacttcat gggcgataac gtacccctgg
   105181 gcaaaatcgc catccgcgcc aagtttgtct ttcatgtcat catagaagga gagatcgata
   105241 taaactgtgc catccgccgg gcaatagaac ggccccataa tggactggcc cgccccgcag
   105301 ccggtgcgcg tcattccacg gtacatgacc agcttcggtt gctgataggt cttacccatc
   105361 ttttcgaact gttgtcccca ggtgtcttcc gtggttgcct gaatcaccga ggtgaatttt
   105421 gcggcttcgt cttcattagg gctaattgac cgcgttgatt gttgttgaga aaccggctgc
   105481 ccggtcatca acccggttaa atcaacgcca tagtagcctg caaccagcac gactatcagt
   105541 aaaatcagcc cgcctttacc gctcggcaga cggaaaccgg gaccgcccat tgacggaccg
   105601 ccagagctgt tgcgcctgtc ttcaacattg tcactttcac gtcgcccttg ccaacgcata
   105661 gatacctcaa caatatattc attatagaaa tgatcgtagg tggttcagag gaagattacc
   105721 acaggaaagc gggatgaaat ggcggaggaa tgagcgttca atgcatatat tccgagagcg
   105781 gttgtcttgc cgtgccagct gcacggcaag atgatgaaca gaaaaatcag tccagctgta
   105841 cacccaggcg gcgggcgacg gcttcatagg cttcgatcag gccaccgagg ctctggcgga
   105901 aacggtcttt gtccattttc tccagtgttt ctttgtccca caggcggctg ccgtccgggg
   105961 agaactcatc gcccagtacc acttcacctt tgtacagacc aaactccagc ttgaagtcga
   106021 ccagaatcag accagcatca tcgaacagtt ttttcagcac gtcgttcgct ttgtaggtca
   106081 gctctttcat acgcgccagg ttctctttgc tcacccagcc aaaggtttcg cagtaagatt
   106141 cgttgaccat cggatcgtgc atggcatcgt ttttcaggaa cagatcgaac agcggcgggt
   106201 tcagctcaat accttcttcg attccaagac gtttcactag agagccagca gcacggttac
   106261 gcacgacaca ctcaaccggc accatatcca gctttttcac cagacattcg gtatcggaga
   106321 gcagacgctc catttgagtc gggatacccg cttcagccag tttgctcata atgaagtagt
   106381 tgaacttgtt gttcaccata cctttgcgat caaactgctc aatgcgcgcg ccatcccctg
   106441 ctgacgtatc attgcggaat tcgagcacca acaggtccgg gttttccgtg ctgtatacgg
   106501 ttttcgcttt accacgatac aactcagctt gcttttgcat ctttatcact cctgggtgtg
   106561 aattaacgtt ttaaaatctt ttgctgtctg gtgtgccgga tgttttgtcg gatgcggcgt
   106621 gaacgcctta tccgaactac gtccgacaaa caaatttcgt gcgaattacc gctaaaatcg
   106681 cgttttcctg ccgacgcaca cgtttgcgta tcatatcaga aaaaagggcc ggatgattcc
   106741 agccctgtat ttttacttgc taaacgcagc ctggaagaca gctaccagcg cgtcgttctg
   106801 actctgagtc agagtatgac ctttcggatc gatgaactgt aggctgctgc ggttatctaa
   106861 atcgccaacc tgcagtttat agtcaccgga tgccaggcct ggatcgctcg cgcccagttc
   106921 ctgccagtcg ctgtcagaca gcggcttata agttacggcc atgttgccct gcgaacgggt
   106981 gctgtcggtc actttcatgc ccactttttc cagcgccgct ggcagacgtt gccaaaccac
   107041 attgaacggc ccgcgtacga ccagcattgg taaaccggtg tcatcagctg cactttgtac
   107101 gtccatagtg gtggaggcac gattttgcgc agcgttcgcg gcgtcagtgg cagatttatc
   107161 cagaccggcg gaaataacgt tcatcatctc cgtgctgtaa cgctgcatgg aagccgcgtc
   107221 tgcaaccggt ttgcccgcct gttccaggtt cagcagttta accgtaaccg cctgctgata
   107281 accctgcggc ttaacagaga tttgataacg accacgatac tgctcgtctt cgtccagacg
   107341 gttccattgt acccaatcgg tggtcagtgt ctgaccagca tcatcacgtt gggtgatggt
   107401 gtagtttttc gcctgcagca cgctaaccac ctgcggccac agagtattgc cacgaccatt
   107461 ttccaccagc aatgaagcgg tatcgcccgt gaactgggta cgcgcgccag aaaccagtgc
   107521 caacggctgg gctggtggac gaatgtccag cgccttaccg acagcaccac taccgttggt
   107581 caccgggatt gcataatcac cggaggtcac cggcaaaatc attccagccg gggcatgaag
   107641 ctccgcaagc ggtgccgctt ccaggtaggc ttcatcacca ctgacctgac gcttatagcg
   107701 tgagtcagaa ctacaggcag cgagtaataa aacaagcgaa acacccgcaa cctttgccag
   107761 gcgcgacttt tgaacagagt aagccatcaa atctccctaa actttacagc aaaccggcat
   107821 gcttaagcgc cgctctgacc gtctcacgac cactgtcggt gattggtgtc attggcaggc
   107881 gcagcgtatc ggtcgccaca agacccagtt ccttacatgc ccatttcacc gggattggat
   107941 tgggttcgac aaatagtttg ttgtgtaatg gcatcagacg ctgattaata acgcgtgcct
   108001 cggcaaaatg cccttctgct gccagtttgc acatctgggc catatcacgc gctgcgacgt
   108061 tagccgtaac ggaaataact ccatgaccgc ccaattgcat gaagtccagc gcgctcgcat
   108121 catcgccgct cagcagaaca aaatcatctg aaaccagctc tttgatctgg tttacacgcg
   108181 ttaagttccc tgttgcctct ttgattccga taatattttt tactttcgcc agacggccca
   108241 ccgtttccgg gagcagatcg cagccagtac gggacggcac attatacaga atttgcggca
   108301 ggtcagtatg ctcagcgatg gctttgaaat gctgatacaa accttcttgc gacggacgat
   108361 tgtagtaagg ggttaccgtc aggcagccga cgataccact gtcattgaag cgctgcgtca
   108421 ggctaatggc ttccgcagta gcgttagcgc cggtcccggc aattaccgga atgcgcccat
   108481 cagccagatc cagcgtcatc atcaccacat cagcatgttc gtcatgattt aaggtagcgg
   108541 actcgccagt ggtgccaaca gaaacgatcg ccgaagtacc gctggcgaca tgataatcaa
   108601 tcagtttttt caagctagcc cgacagacat tacctttttc atccatcgga gtaacaatcg
   108661 cgacaatact tcccgtgaac atgggccatc ctctgtgcaa acaagtgtct caatggtacg
   108721 tttggtatgg cattaaaagc aagcagacag aaccgttctg attgttgtat gcatgatttt
   108781 tttatgcttt ccttaagaac aactcacccc ttaaaggaat aaccagtttg acactgtcat
   108841 cgcaacatta tctggtgatc actgcgttgg gtgccgatcg ccctggaatt gtgaacacca
   108901 tcacccgtca tgtcagtagt tgcggctgta atattgaaga cagtcgcctg gcgatgctgg
   108961 gagaagagtt cacgtttatc atgctgcttt ccggttcatg gaatgccatc actctgattg
   109021 aatcaacgtt accgttgaaa ggtgccgaac tggatctttt aatcgtgatg aagcgcacga
   109081 cggcgcgtcc gcgtccgcca atgccagcat ctgtctgggt tcaggtcgat gttgcagact
   109141 ccccgcattt aattgaacgc ttcacagcac ttttcgacgc gcatcatatg aacattgcgg
   109201 agctggtgtc gcgcacacaa cctgctgaaa atgaacgggc tgcgcagttg catattcaga
   109261 taaccgccca cagccccgca tctgcggacg cagcaaatat tgagcaagcg ttcaaagccc
   109321 tatgtacaga actcaatgca caaggcagta ttaacgtcgt caattattcc caacatgatg
   109381 aacaggatgg agttaagtaa tgaatccact gaaagccggt gatatcgcac cgaaatttag
   109441 cttgccggat caagacggag aacaagttaa tttgaccgac ttccagggac agcgtgttct
   109501 ggtttatttc tacccgaaag ccatgacccc cggctgtacc gtacaggcct gcggcttacg
   109561 cgataacatg gatgagttga aaaaagcggg cgttgatgtg ctgggtatca gcaccgataa
   109621 acccgaaaaa ctctcccgtt ttgcggaaaa agagctgctt aactttacgc tcctgtctga
   109681 tgaggaccac caggtgtgcg aacaattcgg cgtctggggt gaaaagtcct tcatgggcaa
   109741 aacctacgat ggtattcatc gcatcagctt cctgattgac gctgatggca aaatcgaaca
   109801 tgtctttgac gatttcaaaa ccagcaatca ccacgacgtt gtgctgaact ggctgaaaga
   109861 acacgcctga ttactttgct ccattccgtg ctggctgcgc ttgcggccag catacctcac
   109921 ttctcgtgat caagatcaca ttctcgcttt cccctgcgac acgggtgtcg aatccatttt
   109981 ttgctgaacg ttaatgacca tcatttttgt accgttcaga atccagttaa tacataactt
   110041 attgaataga ttgagttaat cagaatggca tcctttatac aatatgaaat gcaatgtttc
   110101 atatcatttt caaggagccg acatgaaccg ctttgtggtg gccgaaccac tgtggtgtac
   110161 aggatgtaat acctgtctcg ctgcctgttc ggacgtgcat aaaacgcaag gtttacagca
   110221 acacccgcgc ctggccctgg cgaagacgtc aacaatcact gcccctgtcg tgtgtcatca
   110281 ctgtgaggaa gccccttgcc tgcaggtctg cccggtcaat gccatctctc agagggatga
   110341 tgcgatccaa ctcaacgaaa gcctctgtat tggctgcaag ctttgcgccg tggtctgccc
   110401 atttggcgca atcagcgctt caggaagccg tccggtgaat gcccatgcgc aatatgtttt
   110461 tcaggctgaa ggctcactca aagacggcga agaaaacgtg ccgccccaac atgcgttgct
   110521 gcgctgggaa ccgggtgtcc agaccgtcgc ggtgaaatgc gaccagtgtg atttcttgcc
   110581 agaaggtccg gcctgcgttc gcgcttgccc gaatcaggcg ttacggctga tcaccgacga
   110641 tagcctgcaa cgtcagatga aagaaaaaca gcgccttgcc gcaagctggt ttgccaatgg
   110701 cggggagtct cccctttccc tcactcagga gcaacactaa tggatgccct gcaattatta
   110761 acctggtcgc tgatcctcta tctgtttgcc agtctggctt cgctgttttt actcggtctg
   110821 gacagactgg ctattaagct ttccggcatc acatcgctgg tgggcggcgt gattggcatc
   110881 atcagcggaa ttacgcaatt acatgctggt gtaactttag tcgcccattt tgccacccct
   110941 tttgactttg ccgatttaac cctgcgaatg gatagcctct cggcatttat ggtgctagtt
   111001 atctccttgc tggtggtggt ttgttcgctc tattcattga cttatatgcg cgaatacgag
   111061 ggcaaaggcg cggcggcgat gggcttcttt atgaatcttt tcatcgcatc gatggttgcc
   111121 ctgctggtga tggacaacgc tttttggttc atcgtgctgt ttgaaatgat gtcgctgtct
   111181 tcctggtttc tggtcattgc caggcaggat aaaacgtcga tcaacgctgg catgctctac
   111241 ttttttatcg cccacgccgg atcggtgctg attatgatcg ccttcttgct gatggggcgc
   111301 gaaagcggca gcctcgattt tgccagtttc gcacgctttc actttctccg gggctggcgt
   111361 cggcggtgtt tctgctggcc tttttcggtt ttggcgcgaa agccgggatg atgccgttgc
   111421 acagctggtt gccgcgcgct caccctgccg caccatcgca cgcttcggcg ttgatgtctg
   111481 gcgtaatggt caaaatcggt attttcggca tcctgaaagt agcgatggat ctgctggcgc
   111541 aaacgggttt gcctctgtgg tggggcattc tggtgatggc gatcggcgca atctccgcgc
   111601 tcctgggcgt gctatatgcg ctggcggaac aggatatcaa acggctgctg gcctggagta
   111661 ccgtcgaaaa cgtcggcatt attttgctgg cagtcggtgt ggcgatggac ggtctgtcac
   111721 tgcacgaccc gctgctcacc gtggttggac tgctcggcgc actgtttcat ctgctcaacc
   111781 atgcgctgtt caaagggctg ctatttctcg gcgcggacgc gattatttcg cgtttgcata
   111841 cccacgacat ggaaaaaatg ggggcactgg cgaaacggat gccgtggaca gccgcagcat
   111901 gcctgattgg ttgcctcgcg atatcagcca ttcctccgct gaatggtttt atcagcgaat
   111961 ggtacacctg gcagtcgctg ttctcactaa gtcgtgtgga agccgtagcg ctacaacttg
   112021 cgggtcctat tgctatggta atgctggcag tcactggtgg gctggcagta atgtgcttcg
   112081 tcaaaatgta cggtattact ttctgtggtg cgccgcgcag tacacacgct gaagaggcac
   112141 aggaagtgcc aaatacgatg atcgtcgcca tgctactgct cgcggcactc tgcgtattaa
   112201 ttgcgcttag tgccagttgg ctggcaccga agataatgca tattgcccat gcgtttacca
   112261 atacccctcc cgtcactgtc gccagcggaa tagcacttgt acccggcacg tttcatacac
   112321 gggtcactcc ttcattactg ttgctgttac tactggcgat gcctttgctg ccaggccttt
   112381 actggctgtg gtgtcgttcg cgccgcgcag cgtttcgtcg cacaggagat gcctgggcat
   112441 gcggctacgg ctgggaaaac gcgatggctc cgtcaggcaa tggcgtgatg cagccgctgc
   112501 gtgtggtctt ctctgcgtta tttcgtctac gacaacagct cgaccctacg ctgaggctaa
   112561 ataaaggtct tgcgcacgtc accgccaggg ctcagagcac agaacccttc tgggatgagc
   112621 gggtgatccg ccccatcgtg agcgccaccc aacggctggt caaagaaata cagcatctgc
   112681 aaagcggcga ctttcgtctc tattgcctgt atgtggtcgc cgcactggtt gtgctgctaa
   112741 tcgctattgc cgtctaagga aatcaccatg agacaaactc tttgcgacgg atatctggtc
   112801 atttttgcgt tagcacaggc cgtgattctg ctgatgctaa ccccactttt tacgggtatt
   112861 tcccggcaga tacgcgcgcg tatgcactcc cgccgcgggc cggggatctg gcaggattat
   112921 cgcgatatcc acaaactgtt taaacgccag gaagttgcgc cgacatcttc aggtctgatg
   112981 ttccgcctga tgccgtgggt attaatcagc agcatgctgg tgctggcgat ggccttacca
   113041 ctatttatta ccgtttcccc ttttgcgggc ggcggcgatc tgatcaccct tatctatctt
   113101 cttgccctgt ttcgtttttt ctttgctctt tccgggctgg ataccggaag tccgtttgcg
   113161 ggagtcggtg ccagtcgcga gttgacgctc ggcattctgg tcgaaccaat gcttattctc
   113221 tcactgctgg tattggcgct gatagcaggt tccacgcata tcgagatgat cagcaatacg
   113281 ctggctatgg gctggaactc gccgctaacc accgtactgg cgttactggc ctgtggtttt
   113341 gcctgcttca ttgagatggg aaaaattccc tttgatgttg ctgaagcaga acaggaatta
   113401 caggaaggcc cgctgaccga atattccggt gccgggctgg cgctagcgaa atgggggctg
   113461 gggctgaaac aggtcgtgat ggcagcactg tttgtggccc tgtttctgcc ctttgggcgc
   113521 gcgcaagaac tttctctcgc ctgcctgctg acttcacttg tcgttacgct gctcaaggtt
   113581 ttgctgattt ttgtactggc ctcaatcgca gaaaacacgc tggcacgcgg gcgtttttta
   113641 ctcattcacc atgtgacctg gcttggcttc agccttgctg cgcttgcatg ggtcttttgg
   113701 ttaaccggtc tgtaaggagc actgacggaa tatggaaaat cttgctctga cgacgttaat
   113761 gctgcctttt atcggcgcac tggtcgtttc gttttcgcca caacgtcggg ccgccgaatg
   113821 gggggttttg ttcgccgcgc tgaccacgct gtgcatgttg tcgctgatct ccgcgtttta
   113881 tcaggccgat aaagttgccg tcacgttgac gttggtcaac gtgggggatg tggcattatt
   113941 tggcctggtc attgatcgca tgagtacgct gattctgttt gtggtggtgt ttctcggttt
   114001 gctggtcacg atctactcca cgggttatct gacggataaa aatcgcgaac acccgcataa
   114061 cggcacgaat cgttattacg catttttgct ggtgtttatc ggcgcgatgg cgggactggt
   114121 actctcctcg acgctgctcg gtcagttgtt gttttttgaa attacgggcg gctgctcctg
   114181 ggcgttgatc agttattacc agagcgataa agcgcagcgt tcagcactaa aagcgttgct
   114241 tatcacccat atcggttcgt tggggttgta tcttgccgcc gccacgctgt ttttgcagac
   114301 cggaacgttt gtgcttagcg cgatgagcga gttacatggc gacgcacgtt atctggttta
   114361 tggcggcatc ctgtttgccg cgtgggggaa atcggcccag ctaccgatgc aagcgtggct
   114421 accggacgca atggaagcgc caacaccgat cagcgcctat ctccacgccg catcgatggt
   114481 gaaagtgggc gtttacattt ttgcccgcgc cattatcgac ggcggcaata tcccgcatgt
   114541 gattggcggc gttggcatgg tcatgacact ggtcaccatt ctttatggct ttctgatgta
   114601 tttgccacag caggatatga agcggttgct ggcctggtcg accatcactc aacttggctg
   114661 gatgttcttc ggcttgtcgc tctccatctt cggctcgcgg ctggcgctgg agggtagcat
   114721 cgcctacatc gtcaaccacg cgttcgctaa aagcctgttt ttccttgtag caggtgcgct
   114781 gagttacagc tgcggcacgc gcttgttgcc gcgtctgcgt ggcgtattgc acaccctgcc
   114841 gttgccaggc gtgggtttct gcgtggcagc gctggcaatt accggtgtgc cgccgttcaa
   114901 cggcttcttc agtaaattcc cgctgtttgc cgccggtttt gcgttgtcag tggagtactg
   114961 gatcctgctg cccgccatga ttctgctgat gattgaatcg gtcgccagtt tcgcctggtt
   115021 tattcgctgg tttggtcgcg tcgtgccggg caaaccgagc gaggccgtcg ccgatgccgc
   115081 accgctgcca gggtcaatgc gcctggtgtt gattgtactg attgtgatgt cgctgatttc
   115141 cagcgtaatc gccgcgacct ggttgcagta aggagatgat gaatgaccgg ttctatgatc
   115201 gtaaataatc tggcgggact gatgatgctg acatcgctgt ttgtgattag cgtcaaaagc
   115261 tatcgcctgt catgcggatt ttacgcctgc cagtcgctgg tgctggtgtc tattttcgcc
   115321 actctctcgt gcctgttcgc cgcagagcaa ctgctgatct ggtccgccag cgcctttatc
   115381 accaaagtgc tgctggtacc gttaatcatg acttacgctg cacgaaatat tccccagaac
   115441 atcccggaaa aagcgttatt cggtccggca atgatggcac tgctcgcggc gttaattgtc
   115501 ctgctttgcg catttgtcgt tcagcccgtg aagctaccga tggctaccgg gctgaaaccg
   115561 gcgctggcgg tagcgttagg tcattttctg cttggcctgc tgtgcattgt cagccaacgc
   115621 aatatcctgc ggcaaatttt tggttactgc ctgatggaaa acggctccca tctggtgctg
   115681 gcgcttcttg cctggcgagc accggaactg gtggaaatag gtatcgctac cgacgccatc
   115741 ttcgccgtca ttgtgatggt gttactggca agaaaaatat ggcgtaccca cggcacgctg
   115801 gacgtgaaca acttgaccgc gctgaaggga taatgagatg agttattctg tgatgttcgc
   115861 tttactcctg ctcacgccgc tgcttttttc gctgctctgt tttgcctgcc ggaaacgggg
   115921 gctctctgcg acttgtacgg tgactgtatt acatagctta gggatcacac tgctgctgat
   115981 tctagcactc tgggtggtcc aaactgccgc tgatgcagga gaaatattcg ctgcgggact
   116041 gtggcttcat attgatggtc tgggcggttt gttcctcgcc attcttggtg tgattggctt
   116101 tctcaccggt gtttactcga ttggctacat gcgtcatgaa gtggcacacg gcgagctttc
   116161 acccgttaca ctgtgcgatt actacggttt cttccatctg tttttgttca ccatgctgct
   116221 ggttgttacc agcaataacc tgattgtgat gtgggcggcg atcgaagcca ccaccttaag
   116281 ctcggcgttt ctggtaggca tttacggtca gcgttcatcg ctggaatcag catggaagta
   116341 catcattatt tgtactgttg gtgtcgcttt tggtctgttc ggtaccgtgc tggtatacgc
   116401 caacgccgcc agcgttatgc cgcaggcaga aatggcgata ttctggagcg aggttcttaa
   116461 gcaatcgtcc ttgcttgacc caacattaat gctgttggcc tttgtgtttg tgctaattgg
   116521 ctttggtacc aaaaccgggc tatttcccat gcacgcctgg ctgccggatg ctcacagtga
   116581 agcgccgagt ccggtcagcg ccctgctctc cgccgtattg ctgaactgcg cgctgttggt
   116641 gctgattcgc tattacatca ttatttgcca ggccatcggc agcgatttcc ccaaccggtt
   116701 gttgctcatc ttcggcatgt tgtcggttgc cgtggcggca tttttcattc tggtacagcg
   116761 ggacattaag cgtctgctgg cgtactccag cgtggagaac atggggctgg tcgcggtggc
   116821 gctaggcatt ggcgggccgc tgggaatttt tgccgcgctg ctgcacacct taaaccacag
   116881 tctggcaaaa acgctgctgt tctgcggttc cggcaatgta ctgctcaagt acggcacgcg
   116941 cgatctcaac gtcgtctgtg ggatgctcaa aatcatgcca tttaccgccg tgctgtttgg
   117001 cggcggtgcg ctggcgctgg cgctggcagg gatgccgccc ttcaacattt ttcttagcga
   117061 atttatgacc gttaccgcag gactggcacg taatcacctg ctgattatcg tcctgctgtt
   117121 attgctgtta acgctggtgc tggcgggcct ggtacggatg gctgcgcggg tgttaatggc
   117181 gaaaccgccg caggccgtta accggggtga tctcggctgg ttgaccacct cgccaatggt
   117241 gattctgctg gtcatgatgc tggcgatggg aacgcatatt ccacaacctg tcatcaggat
   117301 cctggcgggc gcttccacta tagtcctctc agggacgcac gacctgcctg cacaacgtag
   117361 cacctggcat gattttttgc cttcaggcac cgcatctgtt tcggagaaac acagtgaacg
   117421 ttaattcatc gtcaaatcgt ggcgaagcga ttctcgccgc cctgaaaacg cagttccccg
   117481 gcgcggtgct ggatgaagag cgacaaacgc ctgaacaggt caccattacg gtgaaaatca
   117541 atctgctgcc tgacgttgta cattatcttt attatcaaca tgatggctgg cttccagtcc
   117601 tgtttggcaa cgacgagcgg acacttaacg gtcattacgc ggtttattat gccctttcaa
   117661 tggaaggggc cgaaaaatgc tggatcgtgg tgaaggcact ggtcgatgcc gacagtcggg
   117721 agtttccgtc agtcacaccg cgcgtccctg ccgcggtctg gggcgagcga gaaattcgcg
   117781 atatgtacgg gctgattccg gttggcctgc cggatcagcg tcgcctggtg ttgcccgatg
   117841 actggccgga agatatgcat ccgctgcgca aagatgcgat ggattatcga ctgcgccctg
   117901 aaccgacgac agattccgaa acgtatgcgt ttatcaatga gggcaacagc gatgcgcggg
   117961 tgatccctgt cggcccgctg catatcacct cctatgaacc gggtcacttc cgcttgtttg
   118021 tggatggcga gcaaattgtc gatgctgatt accgcctgtt ttatgtccat cgcggcatgg
   118081 agaaactggc agaaacgcgg atgggctaca acgaagtgac cttcttatcg gaccgcgtgt
   118141 gtgggatttg cggttttgcc cacagtgtgg cctataccaa ctcggttgaa aatgcactgg
   118201 ggattgaggt gccgcaacga gcacatacta ttcgctcgat tctgctggaa gtcgagcggc
   118261 tacacagtca tttgctcaac cttggcctct cctgccattt tgttggtttt gataccggct
   118321 ttatgcaatt tttccgcgtg cgggaaaagt cgatgacgat ggcggaattg ctgaccgggt
   118381 cgcgtaaaac ctacggtctg aatctgattg gtggtgttcg ccgcgatatt ctcaaagagc
   118441 aacgtctgca aacgctgaaa ctggtgcgcg agatgcgcgc cgacgtgtcg gagctggtag
   118501 agatgctgct tgctacgccg aatatggaac aacgcactca gggcattggc attctcgacc
   118561 gacaaatcgc ccgtgattat agccctgtag ggccgctgat ccgcgcagtg gttttgcccg
   118621 tgatttgcgc tttgatcacc cctacgccga ctacggcaat attccaaaaa cactgtttac
   118681 ctttaccggc ggcgatgttt tctcccgcgt gatggtccgt gtcaaagaga cgtttgattc
   118741 gctggcaatg ctggaatttg ccctcgacaa catgccggat accccactgc tgaccgaagg
   118801 ctttagctat aaacctcacg cattcgcgct gggctttgtt gaagcgccac gcggtgaaga
   118861 cgtgcactgg agcatgctcg gtgataacca aaaattgttc cgctggcgct gtcgtgccgc
   118921 cacctacgcc aactggccgg tgttgcgtta catgctgcgc ggcaataccg tttctgacgc
   118981 accgctgatt atcggtagcc ttgatccctg ctactcctgt accgaccgtg tgacgctggt
   119041 agatgtgcgc aagcgccagt caaaaaccgt gctgtataaa gagatcgaac gctacggcat
   119101 tgatcgtaac cgttcgccgc tgaagtaagg acagaagatg ctgaagttac tgaaaactat
   119161 tatgcgcgcc ggaaccgcga cggtgaaata tcccttcgcg ccactggagg tcagcccagg
   119221 ctttcgcggg aaaccggacc tgatgcccag ccaatgtatt gcctgcggtg cctgcgcctg
   119281 tgcttgtccg gcaaatgcgc tgactatcca gaccgacgac cagcaaaatt cgcgcacctg
   119341 gcagctctat ctggggcgtt gtatttactg cggacgttgt gaagaagtgt gcccgaccag
   119401 agccatccag cttaccaata actttgaact gaccgtcacc cataaagccg atctctatac
   119461 ccacgcgacg ttccatctac aacgttgcag ccgttgcgaa cgcccgtttg ccccgcaaaa
   119521 aaccgtcgca ctggctgctg aattgttggc acagcaacaa aatgctccac aaaaccgcga
   119581 aatgttatgg gcgcaagcga gcgtctgccc ggagtgcaaa caacgcgcaa cgctgatcaa
   119641 cgacgataca gatgtaccgc tggtggctaa ggagcagcta tgagtccagt gcttacgcaa
   119701 tatgtcagcc agcccatcac gctggacgag caaacgcaaa agatgaagcg gcatttgcta
   119761 caggatatcc gtcgctcggc ttacgtttat cgcatcgatt gcggcggctg caacgcctgt
   119821 gaaatcgaaa tttttgctgc catcaccccc gtattcgacg cagaacgttt tggcattaag
   119881 gttgtttcat caccgcgtca cgccgatatt ttgttattta ctggcgcagt cacccgggcg
   119941 atgcgtatgc ctgcacttcg ggcgtatgag tctgcccccg atcataaaat ctgtgtttcc
   120001 tacggcgcgt gcggtgtcgg cggcggtatt ttccacgatc tctacagcgt ctggggcggc
   120061 agcgacacca ttgtccccat tgatgtttgg atccccggct gcccgccaac accggccgcc
   120121 accattcacg gtttcgccgt ggcgctcggt ttgctgcaac agaagattca cgcggtggat
   120181 tatcgcgatc ccaccggggt gactatgcaa ccgttgtggc cgcagatccc gccatcacag
   120241 cgtatcgcca ttgagcgaga agcgcggcgg ctggcgggct atcgtcaggg gcgagaaatt
   120301 tgcgatcggc tcctgcgcca tttaagcgac gatcctacag gaaatcgggt taacacctgg
   120361 ttgcgcgatg ccgacgatcc acgtctcaat agtatcgttc agcaactctt tcgcgtactc
   120421 cgggggttac atgactgaag agtgcgggga aattgttttc tggacgctgc gaaaaaagtt
   120481 tgtcgccagt agcgacgaga tgccggaaca cagctctcag gtaatgtatt actcgctggc
   120541 tatcggtcat cacgttggcg tgattgattg tctgaatgtc gccttccgct gcccactgac
   120601 ggaatacgaa gattggcttg cactggtcga agaggagcaa gcccgacgta agatgctggg
   120661 ggtgatgact tttggtgaga ttgttattga cgccagccac accgccctgt tgacccgggc
   120721 attcgcgcca ctggcagatg acgcgacgtc tgtgtggcaa gcgcgtagca ttcaattcat
   120781 tcatctgttg gatgaaattg tgctggaacc ggctatctat ctgatggcca gaaaaattgc
   120841 gtgagaagga tttctcatta ataaggactg ttgatggcta tgtcagacga agcgatgttt
   120901 gccccgccgc aaggaataac aattgaagcg gtaaacggaa tgctcgcgga gcggttagcg
   120961 cagaaacacg gcaaggcgtc tttattacgc gccttcatcc cgctgccgcc gccgttcagc
   121021 ccggtacaac ttattgaact gcatgttctc aaaagcaact tctattaccg ctaccatgat
   121081 gatggcagcg atgtgacggc aacaacagag tatcagggcg agatggtcga ttattcgcgt
   121141 tacgccgtcc ttctcggcag tagtggaatg gcggagctac gctttattcg tacccacggc
   121201 agccgtttta ctccccagga ttgcacactg tttaactggc tggcacgcat tatcaccccg
   121261 gttctgcaat catggctcaa tgatgaagca cagcaggtgg cgctgcgttt gctggagaaa
   121321 gatcgcgatc atcatcgggt actggtggat atcactaatg cagtgctgtc acatcttgat
   121381 ctcgacgatc tgatcgctga cgtcgctcgt gagatccatc attttttcgg tctggcttca
   121441 gtcagtatgg tactgggcga tcatcgaaag aacgagaagt tcagcctgtg gtgcagcgat
   121501 ctttctgcct cacattgtgc gtgtctgcca cacaatatgc ctggcgacag tgtattgctg
   121561 acacaaacgc tacaaacccg acaaccgacc ttgacgcacc gtgcagatga tctgtttctc
   121621 tggcaacgcg acccgttatt actcttactt gcatctaacg gctgcgaatc tgcgctcctt
   121681 ataccgctta cctttggcaa ccatacaccg ggtgcattgt tgctggcgca tacctcttcc
   121741 actctcttta gtgaggaaaa ctgccagcta ctacaacaca tagccgatcg catcgctatt
   121801 gccgttggca atgccgatgc ctgccgtaga atgaccgatt tgcaggaaag tttgcagcaa
   121861 gaaaaccacc agcttagcga gcagctcctt tcgaatctgg gcgtcggtga cattatctat
   121921 caaagccagg caatggaaga cctgctccag caggtagata ttgtggcgaa gagcgacagt
   121981 acggtgttga tttgcggtga aaccggaacc ggcaaagagg tgatcgccag agcgatccat
   122041 caacttagcc cgcgacacga caagccgctg gtcaaaatca actgcgctgc catccccgcc
   122101 agtcttctgg aaagtgagtt attcggtcat gacaaagggg cgtttactgg tgcgattaat
   122161 acccatcgtg gtcgttttga aattgccgat ggcggcacgt tgtttctcga tgaaattggc
   122221 gatctgccgt tagaacttca gcctaaactg ctgcgcgtat tgcaggaacg ggagattgag
   122281 cgtctcggcg ggagtagaac gatcccggta aatgtcagag tcattgccgc caccaaccgt
   122341 gatttgtggc aaatggttga agatcgccag tttcgcagcg atctctttta tcgcctgaat
   122401 gtcttcccac tggaattgcc tccgctgcgc gaccgtccgg aagatatccc tcttttagca
   122461 aagcatttca cgcaaaaaat ggcgcgccat atgaatcgtt caattgacgc catcccgacc
   122521 gaggcgctac gccagttgat gtcgtgggac tggccgggca acgtgcgcga gctggaaaac
   122581 gtgattgagc gagcggtact gctgactcgc ggtaacagtc tgaatttaca tcttaatgtc
   122641 cgacaaagcc gtttactgcc gacgctaaat gaagattcag cgcttcgcag ttcaatggcg
   122701 tagttgctgc acccgacgac gccagagaat gacgaagaag aacgtcagcg cattgttcag
   122761 gtattgcgag aaaccaatgg cattgttgcc gggccccgtg gcgcggcgac acgattaggg
   122821 atgaagcgca ccacgctgct gtcacgaatg cagcgtctgg gaatctcggt tcgcgaggtg
   122881 ttgtaatctg cttttgcagg agtatgcatg agaaacaaac tctctttcga cttgcagttg
   122941 agcgccagaa aagcggcaat cgctgaacgg attgccgccc ataaaattgc ccgcagtaaa
   123001 gtgtcggtct ttttaatggc gatgtccgct ggcgtgttta tggcgatgtc cgctggcgtg
   123061 tttatggcga tcggatttac tttttacctt tccgttatcg ccgatgcccc gtcttcatag
   123121 gcattaaccc atctggtggg cggcctttgc tttacacttg gctttatttt gctggcggtt
   123181 tgcggcacca gcctgttcac ctcgtcggta atgacggtaa tggcaaaaag tcggggcgtt
   123241 attagttggc gaacttggct gattaacgca cttctggtgg cctgcggtaa tctggcaggt
   123301 attgcctgtt tcagtttgtt aatctggttt tccgggctgg tgatgagtga aaacgcgatg
   123361 tggggagtcg cgattttaca ctgcgccgag ggcaaaatgc atcatacatt tactgaatct
   123421 gtcagcctcg gcattatgtg caatctgatg gtttgcctgg cgctgtggat gagttattgc
   123481 ggacgttcgt tatgcgacaa aatcgtcgcc atgattttgt ccatcaccct gtttgtcgcc
   123541 agtggctttg agcactgtat cgccaatttg tttgtgattc cgttcgccat tgccattcgc
   123601 catttcgccc ctacctcctt ctggcaactg gcgcacagta gcgcagacaa ttttccggca
   123661 ctgacggtca gccattttat taccgccaat ctgctcccgg tgatgctggg taatattatc
   123721 ggcggtgcag tgctggtgag tatttgttat cgggctattt atttacgtca ggaatcctga
   123781 aaaatcagcc cggcgaaaca gttcgtcggg ctgaagatta ttcttgcgcg atttgcccat
   123841 cgggccaggc gtggatcacg gctttgatca gcgtcgccaa tggaatggcg aagaatacgc
   123901 cccagaatcc ccacaaacca ccgaagatca ccaccgataa aataatcacc agcggatgca
   123961 ggttaaccgc ttcggagaac aacaccggta ctagcaggtt gccgtccagc gcctgaataa
   124021 tcagatacac cgcgaagcag ctccagaatt cagtacctgc gccaaactgg aatagcgcca
   124081 cgccaaccac cggaatggtc accacaaatg cgccaatgta cggaataaga accgagaagc
   124141 cgaccagcac tgccagcagc agcgaatagt tcagcccgaa gagcaagaac cccagccagg
   124201 tggcgatccc caccacgatc atctccagca ctttgccgcg gatatagttg gtgatttgtt
   124261 gattcatctc cttccacacc tgtcctgcca gcccacggtt acgcggcagc acccggcgaa
   124321 cggcgttcag catctgctct ttgtctttca gcaggaagaa gaccatcaat ggcaccagca
   124381 ccagatagac ggctatggtc agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg
   124441 aatcgcccat ggtcagcatc cgactgcgca tattttcggc cattgcatca ataatgcccg
   124501 cgtccattaa caccggatag cggcgcggca acgtggcggc aaagtcagaa agcttattga
   124561 gcatccccgg catatcgcgg attaagtaga tgccctgttg ccaggcgata ggcagtacca
   124621 cgaacgccat cagtagcaat ataccgacaa aaaccaccaa tacaatcgac gtcgcccagc
   124681 ggcgggagca gccaatagat tgcaggcgca cggttggcca ttccagcaaa taagccagca
   124741 caatagccac cagcaacgga gcaagcaggc cactaaagaa aaagataatg ccaaatccgg
   124801 caactaaaat aaccagcaag gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt
   124861 gcatcaacat ttcgagcata aagaccttcc ctgaacctca agagcgggat tgcgatccgc
   124921 aattgtatcg aaatgtcaca aaaaagactt cgctttttat gacggattca ggaaactgaa
   124981 aagtcatttg agtgggctaa tcttcgccgt tacactcaaa ggcggcgagg tgggaacgat
   125041 atttcacagt atcggtcaaa tgactatctt cagaaataca ggatagaggt tatgttcagg
   125101 cagttgaaaa aaaacctggt tgcaaccctc attgctgcta tgaccattgg tcaggtagcc
   125161 ccggcgtttg ccgacagcgc agacaccttg ccggatatgg gaacctccgc aggaagcacg
   125221 ctttccattg gtcaggaaat gcagatgggc gactattatg tccgccagct acgcggcagc
   125281 gcgccgttaa ttaatgaccc gctgttaacg caatatatta attcgctggg gatgcgtctg
   125341 gtttcgcatg ccaattcggt taagacaccg tttcattttt ttctgatcaa caacgacgaa
   125401 attaacgcct ttgctttctt tggcggcaac gtggtgctgc actctgccct gttccgttat
   125461 tccgataacg aaagtcaact ggcttcagtt atggcgcacg aaatctccca cgtcacccaa
   125521 cgtcacctgg cgcgagcgat ggaagatcaa caacgcagcg cgccgctgac ctgggtcggc
   125581 gcgttaggtt ctattttact ggcgatggcc agtccgcagg cggggatggc ggcgctgacc
   125641 ggtacactgg cgggaacgcg ccaggggatg atcagtttca cccagcaaaa tgaacaggaa
   125701 gcggaccgca ttggtattca ggtgctgcaa cgctcgggat tcgatccaca ggcgatgccg
   125761 accttcctgg aaaaattgct cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta
   125821 ttgactcacc cgttgccgga aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg
   125881 cgcccgatgg tggtgcagtc gtcggaagat ttctatctgg cgaaagcgcg cacactgggg
   125941 atgtataatt ccggacgtaa ccagctcacc agtgatttgc tggatgaatg ggcgaaagga
   126001 aacgttcgtc agcaacgagc ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat
   126061 aaatacgacg aggcgcgtaa aacgctgcaa ccgttactgg cggcagaacc tggcaacgca
   126121 tggtatctcg atctggctac cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc
   126181 aatcgcctga aaaatgcccg cgatttacgc accaatccgg tgttgcagct caacctggcg
   126241 aacgcttatc tgcaaggcgg tcaaccacaa gaagcggcca atattctgaa tcgctacacc
   126301 tttaataata aagatgacag caacggctgg gatttgctgg cacaggcgga agccgcgcta
   126361 aataaccgcg atcaggagct ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc
   126421 gatcaggcca tttcgctgtt gagtagcgcc agttcgcagg tgaaattagg cagcctgcaa
   126481 caagcgcgtt acgatgcgcg catcgaccag ttgcgccagc tgcaggaacg ctttaagcct
   126541 tataccaaga tgtaattcgc attaaaggaa gaacaatgac caaacaggta aaaatctacc
   126601 ataacccacg ctgttcaaag agccgggaaa cgctgaacct gctgaaagaa aacggcgtgg
   126661 agccagaagt ggtgctctac cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc
   126721 tgaaaatgct ggggatgaat agcgcccgtg aattgatgcg ccagaaagag gatctttata
   126781 aagaactaaa cctggctgac agttcgctta gcgaagaggc gctgattcag gcaatggtgg
   126841 ataatccgaa gctgatggag cgcccgatta tggtcgcgaa tggcaaagcg cggattggtc
   126901 gtccaccgga gcaggtactg gaaatcgtgg gttaagatgt gctccatcgc tagttgaagc
   126961 acattgccgg atgcgacgct tgccacgtct tatccggcct acgaatacta tggattaact
   127021 tcgcgtaggt caggcgttcg cgccgcatcc gacaataaac accttatcta caacttcaga
   127081 atttctttca caaacggaat ggtcagctta cgttgcgcgg taatcgacgc acgatccaac
   127141 tgatccaacg tcataaatag cgtgcgcatt tctctgtcga gccgcttcag caagaaacgc
   127201 cccacatctt ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag cgcctgcaac
   127261 ttatcttcat cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag acgcgacgcg
   127321 agatccggta atcccagatt caactgccgc ggtggacgat cgccggtgat caacaaccgt
   127381 gttttgcccg attccagaat tcgattgtag agatcgaaaa tcgccatctc ccacaactca
   127441 tcgcctgcaa tacactcaat gttgtcgata cagaccagcg acaaatgctc cataccgtcg
   127501 agcacttccg gaacaaacca ggtgcgttta tccagcggga catagcccac cgcatcgcca
   127561 cgctgcgaca attccgcgca agccgcgtgc agcagatggc tgcgccccgc gccttcgcgt
   127621 gcccagagat agatgtaacc gctatgttcc tgacgcagca cgttttgcag cgcggccagt
   127681 aaagaggagt tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg aagataaagt
   127741 ggcaaagaga gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc acaaaatcgc
   127801 gagaagttta ccacagaatc ccatgatgtt tgaaccgggc agcaacactg cccggtcggt
   127861 acattatttg tctgttatat ccgcgtcttc tgcgtccagc accacttctt cagggcgcag
   127921 cacactgatc agtttgaaaa tcaaacttaa gccgataccg acgatggtcg ccagcgccat
   127981 accttttaac tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga tgatcaaaat
   128041 cacggaagtc aggatcaggt tctgtgcttt gttgtaatcc actttcgatt cgatcaaaac
   128101 acgaataccg gaagcaccaa tgacaccata aagcagcagc gaaacgccgc ccatcaccgg
   128161 caatgggatc atctggatag cggcagccag tttaccgacg caggaaagca ggatagcgaa
   128221 aatcgccgcc ccgccgataa cccaggtact gtaaacacgg gtgatcgcca tcacgccaat
   128281 gttttctccg taagtggtat ttggcgtaga gccaaagaag ccggagatca cggtcgacag
   128341 gccgttagca aacatcgaac ggtgcagacc tggatcgcgc agcagatctt ttttgacgat
   128401 attagccgtt actaccaggt gccctacgtg ttcggcaata accactaacg ccgctggcag
   128461 aatagtcaga atggcaaacc actcgaagcg cggcgtatag agggttggca gtgcaaacca
   128521 gtgagcatta ataatcggcg tggtatcgac aattcccatt gcgaaagaga gcgcgtaccc
   128581 caccagcacg ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca gcacggaacc
   128641 taaaaccgtg accgccaggg tggtaataga gatgatgatg gttttggagt ttggcgtttg
   128701 cccttcagcc gggagtaaac ccgccatacc ggcagctacg cccgccagct ccagaccgat
   128761 gacggcaacg attgcgccca ttgccgcagg tggaaacagc acgtccaacc agccggtccc
   128821 cgctttcttc acgataaaag aaaccaggca gaacagcacg ccgcacataa taaagccgcc
   128881 cagcgcgact tcatacccta acggcaacag taacaatacc ggtgaaataa aggcaaagct
   128941 ggaaccaaga taagccggaa ttttcccttt acagatgaag agatacagca gcgttccaat
   129001 accgttaaat aacagtacag tcgccgggtt aatatgaaat aagacgggca ccaggacggt
   129061 tgcaccaaac atggcgaaca aatgttgcaa actaagcggg attgtctgta aaagtggcgg
   129121 tctttcactc accccgatag cacggcgcgt catagtatta tcctctgtat tatgtgttat
   129181 aggcgcttta ctcaaaaaaa agccgactct taaagtcggc tttaattatt tttattcttt
   129241 atttcgtacc aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct
   129301 cgttcagtcc ctggtcaatc gatgcagtat acagttcgac gtccgggtgc gctttttcca
   129361 gcgcagcgat accttctggc gcagcgacca gcaccagaac tttgatgctg ctgcagcccg
   129421 cttttttcag caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa
   129481 cgatcagcgc catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct
   129541 ccagcgtttc ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacgtttt
   129601 ccagcacacc gtccatcata ccaagacccg cacgcagaat tggcacaacg gtaattttct
   129661 tacctttgat ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg
   129721 tttcgaggtc ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc
   129781 gaaagcgctt ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg
   129841 ggtgtttgac ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaat
   129901 cgacggatta tacctccttt cttcagggcg gcaatattat tttcgttgac tttagtcaaa
   129961 atgataacag tttgagataa agttatttta tattcaaatg gttatgaaag aagattattc
   130021 catccgaaaa ctaacctttg ccctggcaca agtcttcttt cgccgcgcgc ccggggaaaa
   130081 gacgtgcaaa aaggttgtgt aaaacagtct cgcaaacgtt tgctttccct gttagaattg
   130141 cgccgaattt tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa
   130201 acctctctta gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga
   130261 agaatcaaag gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc
   130321 ttcggtgcgc tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact
   130381 gacggcgtag gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt
   130441 attgatctgg tcgccatgtg cgttaatgac ctggtggtgc aaggtgcgga accgctgttt
   130501 ttcctcgact attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc
   130561 ggcatcgcgg aaggttgtct gcaatcgggc tgttctctgg tgggtggcga aacggcagaa
   130621 atgccgggga tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt gggcgtggta
   130681 gaaaaatcag aaatcatcga cggctctaaa gtcagcgacg gcgatgtgct gattgcactc
   130741 ggttccagcg gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc
   130801 ggttgtgatc cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca
   130861 ccgacccgca tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc
   130921 attgcgcacc tgaccggcgg cggcttctgg gaaaacattc cgcgcgtatt gccagataat
   130981 acccaggcag tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa
   131041 acggcaggta acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg
   131101 attatcgccc tgcctgctcc ggaagtggac aaagccctcg ccctgctaaa tgccaacggt
   131161 gaaaacgcgt ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc
   131221 gaataatgaa tattgtggtg cttatttccg gcaacggaag taatttacag gcaattattg
   131281 acgcctgtaa aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg
   131341 acgcgttcgg ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca
   131401 gcgcgtttga cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg
   131461 cacccgatgt ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc
   131521 actatgccgg gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac
   131581 acacccatcg tcaggcgctg gaaaatggcg atgaagagca cggtacatcg gtgcatttcg
   131641 tcaccgatga actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg
   131701 gtgatacgga agatgacatc accgcccgcg tgcaaaccca ggaacacgct atttatccac
   131761 tggtgattag ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc gcgtggctgg
   131821 atggtcaacg tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa
   131881 gcgccagctc tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt
   131941 ttttccccct tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg
   132001 cagtgtcccg tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg
   132061 aaaaagagct cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat
   132121 ctaacccgct gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt
   132181 tctataaagt ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct
   132241 ccaacgctca ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc
   132301 aggaattcga cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc
   132361 tgattaatga acgccagctc tccgtcaatc aacaaaactg gctgcgtcat tattttaagc
   132421 agtatctgcg tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgcagt
   132481 tcctgaaaga tgattacacc tatctggcgg tggaaattat ccgtagcgat agcatccgtt
   132541 acgcgctgct ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag
   132601 cgccgcgtcg acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg
   132661 atattttcaa aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc
   132721 gcgatgccga atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt
   132781 ccagtctcaa gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc
   132841 ccaatgcgct ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg
   132901 tccccggcgg tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag
   132961 ccaatctggt gaacaaacca ctgccgcgtt tacgccatat ttggtttgat aaagcccagt
   133021 tccgcaatgg ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca
   133081 cctttgagca tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga
   133141 ttaaaattaa catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg
   133201 ccgcacataa cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt ttcgacgaag
   133261 aagccaacat tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg
   133321 cgccggggct gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag
   133381 tggtccgtta cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata
   133441 ctgactattc gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg cgggtattta
   133501 actttattga aaacccatac cgcccggtga catttgatta tttaatggtg tcaccgcaaa
   133561 actctcgccg tctgttatat gaaatggtag accgcgaaat cgccaacgcg cagcaagggc
   133621 tgcccagtgg tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc
   133681 tgtatgcggc ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc
   133741 tgatccccaa tctggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt
   133801 accttgaaca tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt
   133861 cttccgccga ctggatgacg cgcaatattg attatcgtat tgaagtggcg acaccgctgc
   133921 tcgatccgcg cctgaagcag cgggtgctgg acatcatcga catattgttc agcgatacgg
   133981 tcaaagcacg ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc
   134041 gcaaagtacg ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat
   134101 aaccctatgc caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt
   134161 ggttcaaaca gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt
   134221 ggccgcctga aacagcgggt gcatctggcg gacggcctgg ggccagataa tatgttgagt
   134281 gaagaggcaa tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg gctacaaggg
   134341 ttttctcctg ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc gctgaacgcc
   134401 actgactttc tgaaacgcgc ggaaaaggtc attccctacc cgattgaaat tatttccggt
   134461 aatgaagaag cccgtctgat ttttatgggc gtggaacata cccaaccgga aaaaggtcgc
   134521 aaactggtta ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa
   134581 cctattctcg ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttattttcct
   134641 ggcggggtca tcaataaaga gaattttcag cgcgctcgca tggcggcagc acaaaaactg
   134701 gaaactttaa cctggcaatt ccgtattcag ggctggaacg ttgcaatggg cgcttccggt
   134761 accataaaag ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc
   134821 ccggaacgtc tggaaaaact ggtaaaagaa attttacgtc accgtaattt cgcatcgctg
   134881 agtttaccgg gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta
   134941 tgtggtgtgt ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc
   135001 gaaggcgtac tgtatgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc
   135061 gccagcagcc tcgccaacca gtatcacata gacagcgaac aggcccgacg agtgctggat
   135121 accactatgc aaatgtacga acagtggcgg gaacagcaac cgaagctggc gcatccgcaa
   135181 ctggaggcgc tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa tatcaaccac
   135241 agcggtttgc atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc gggttttaat
   135301 caggaacagc agctgatgat ggcgacactg gtgcgctatc accgtaaagc gattaagctc
   135361 gacgatctgc cgcgctttac cttgtttaag aagaaacagt tcctgccact gatacagcta
   135421 ttgcgccttg gcgtattact aaacaatcaa cgtcaggcaa ccaccacacc gccaacattg
   135481 acactgatta ctgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag
   135541 aatgcgctgg tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc
   135601 tggcggttga aaattgaaga agaaagtacc ccagaaatcg ccgcttaatg ctttgcgggc
   135661 cgacgagaaa tgtcggcccg cattattcag gcactttcgc gaatgggttc gatttcattc
   135721 agcgtatcaa ttaacggttg cggcttacca ataagataac cctgcatata atcgatcccc
   135781 aaagagagca ccgcctcgcg gatctcttcg ttttcaacgt actctgccac tacccgcatt
   135841 ttcttcattc gcgccagatg gcaaatcgac gccacaatct gataatccag actatttgac
   135901 acaatattgc ggataaagct gccgtcaatt ttaagcagat cggcattcac atttttaagc
   135961 cgtgcatagc tggcgtagcc ggtgccgaaa tcatcaatcg caatctggca gcctaattcc
   136021 tgaagatgct gcaaggtgat ttgcgcctgc ttaacattgg tcagagcatt actttcggtg
   136081 acttcaaaaa taagttgcca cgcttcaatc tgatatttag ccagcaactg actgacttca
   136141 acaggaaaac gagcctgaca taccgaggtt ggagacagat taatagcaaa acggtgagcg
   136201 ggcatcttcg ctctgttttc agccataaat tgcagcgtat gctcaatgac ccacatgtcg
   136261 atactcgacg ataaaccaaa ttcgtgcgcg accggcaaga agctaccggg gccgatcagt
   136321 tcatcattct cacctttcat gcgcagaaga atttcatggt aaacatcacc acgcataccg
   136381 gtaatcggct gggccatcag gaaaaaatgg ttgtgttcca gcgcctgctg tagtcgattc
   136441 atcatcgcga ctttatcttt caattcgcgt tgcaaataca ttgccccgca acgctgcata
   136501 ttttccgggg cgttggtcac gatggaaagt tcggcgaccg tatttagctc tcccagcagc
   136561 aggtagatat gattcactgg cgagcgcaca tagcagtaac tgacgccaat ctgcggttgc
   136621 atcggcatcc catcccaaaa gaaacgaaat tgcttgagat gactatccag tgcggtaatg
   136681 cgctcctggt gcgattctgt attcagtcgc agcgcgagat cgttacccga aagctgataa
//
LOCUS       DAJTOW010000002        95534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTOW010000002 DAJTOW010000000
VERSION     DAJTOW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.002"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO244_000132"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO244_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5662109.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO244_000133"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO244_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5662110.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO244_000134"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO244_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5662111.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO244_000135"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO244_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5662112.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO244_000136"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO244_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5662113.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO244_000137"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO244_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5662114.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO244_000138"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO244_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5662115.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO244_000139"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO244_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5662116.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO244_000140"
     CDS             complement(8942..9223)
                     /locus_tag="OO244_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662117.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO244_000141"
     CDS             complement(9237..10898)
                     /locus_tag="OO244_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5662118.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO244_000142"
     CDS             complement(10882..11208)
                     /locus_tag="OO244_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5662119.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO244_000143"
     CDS             complement(11264..11446)
                     /locus_tag="OO244_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662120.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO244_000144"
     CDS             complement(11430..11870)
                     /locus_tag="OO244_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5662121.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO244_000145"
     CDS             complement(11870..12166)
                     /locus_tag="OO244_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5662122.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGD"
     gene            complement(12163..12501)
                     /locus_tag="OO244_000146"
     CDS             complement(12163..12501)
                     /locus_tag="OO244_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5662123.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO244_000147"
     CDS             complement(12498..13709)
                     /locus_tag="OO244_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5662124.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO244_000148"
     CDS             complement(13711..14283)
                     /locus_tag="OO244_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5662125.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO244_000149"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO244_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OO244_000150"
     CDS             complement(15781..16005)
                     /locus_tag="OO244_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662126.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OO244_000151"
     CDS             complement(16147..16308)
                     /locus_tag="OO244_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5662127.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OO244_000152"
     CDS             complement(16318..16623)
                     /locus_tag="OO244_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662128.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OO244_000153"
     CDS             complement(16620..16898)
                     /locus_tag="OO244_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662129.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OO244_000154"
     CDS             complement(17187..18935)
                     /locus_tag="OO244_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5662130.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OO244_000155"
     CDS             complement(18932..19231)
                     /locus_tag="OO244_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5662131.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OO244_000156"
     CDS             complement(19237..19470)
                     /locus_tag="OO244_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662132.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OO244_000157"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OO244_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OO244_000158"
     CDS             complement(19685..19906)
                     /locus_tag="OO244_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662133.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OO244_000159"
     CDS             complement(19896..20096)
                     /locus_tag="OO244_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5662134.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OO244_000160"
     CDS             20226..20405
                     /locus_tag="OO244_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662135.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OO244_000161"
     CDS             20530..20859
                     /locus_tag="OO244_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662136.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OO244_000162"
     CDS             complement(20909..21097)
                     /locus_tag="OO244_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5662137.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OO244_000163"
     CDS             complement(21472..22701)
                     /locus_tag="OO244_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5662138.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWPL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22949..24070
                     /gene="roxA"
                     /locus_tag="OO244_000164"
                     /gene_synonym="ycfD"
     CDS             22949..24070
                     /gene="roxA"
                     /locus_tag="OO244_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5662139.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="OO244_000165"
     CDS             complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="OO244_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5662140.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25595..26731
                     /gene="potA"
                     /locus_tag="OO244_000166"
     CDS             25595..26731
                     /gene="potA"
                     /locus_tag="OO244_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5662141.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26715..27572
                     /gene="potB"
                     /locus_tag="OO244_000167"
     CDS             26715..27572
                     /gene="potB"
                     /locus_tag="OO244_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5662142.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27569..28363
                     /gene="potC"
                     /locus_tag="OO244_000168"
     CDS             27569..28363
                     /gene="potC"
                     /locus_tag="OO244_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5662143.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28360..29406
                     /gene="potD"
                     /locus_tag="OO244_000169"
     CDS             28360..29406
                     /gene="potD"
                     /locus_tag="OO244_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5662144.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="OO244_000170"
     CDS             complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="OO244_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5662145.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="OO244_000171"
     CDS             complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="OO244_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5662146.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="OO244_000172"
     CDS             complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="OO244_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5662147.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="OO244_000173"
     CDS             complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="OO244_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5662148.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="OO244_000174"
     CDS             complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="OO244_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5662149.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34738..35811
                     /locus_tag="OO244_000175"
     CDS             34738..35811
                     /locus_tag="OO244_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5662150.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35939..39385
                     /gene="mfd"
                     /locus_tag="OO244_000176"
     CDS             35939..39385
                     /gene="mfd"
                     /locus_tag="OO244_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5662151.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39528..40490
                     /gene="ldtC"
                     /locus_tag="OO244_000177"
     CDS             39528..40490
                     /gene="ldtC"
                     /locus_tag="OO244_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5662152.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40573..40785)
                     /locus_tag="OO244_000178"
     CDS             complement(40573..40785)
                     /locus_tag="OO244_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5662153.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41070..41702
                     /gene="comR"
                     /locus_tag="OO244_000179"
     CDS             41070..41702
                     /gene="comR"
                     /locus_tag="OO244_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5662154.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41764..42303)
                     /locus_tag="OO244_000180"
     CDS             complement(41764..42303)
                     /locus_tag="OO244_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5662155.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42530..43834)
                     /gene="ndh"
                     /locus_tag="OO244_000181"
     CDS             complement(42530..43834)
                     /gene="ndh"
                     /locus_tag="OO244_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5662156.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44242..44784)
                     /locus_tag="OO244_000182"
     CDS             complement(44242..44784)
                     /locus_tag="OO244_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5662157.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44807..45832)
                     /gene="nagZ"
                     /locus_tag="OO244_000183"
     CDS             complement(44807..45832)
                     /gene="nagZ"
                     /locus_tag="OO244_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5662158.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(45843..46667)
                     /gene="thiK"
                     /locus_tag="OO244_000184"
     CDS             complement(45843..46667)
                     /gene="thiK"
                     /locus_tag="OO244_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5662159.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46648..47289)
                     /gene="lpoB"
                     /locus_tag="OO244_000185"
     CDS             complement(46648..47289)
                     /gene="lpoB"
                     /locus_tag="OO244_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5662160.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47304..47681)
                     /locus_tag="OO244_000186"
     CDS             complement(47304..47681)
                     /locus_tag="OO244_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5662161.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47684..48043)
                     /gene="hinT"
                     /locus_tag="OO244_000187"
     CDS             complement(47684..48043)
                     /gene="hinT"
                     /locus_tag="OO244_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5662162.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48174..48355
                     /locus_tag="OO244_000188"
                     /pseudo
     CDS             48174..48355
                     /locus_tag="OO244_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            48388..50573
                     /gene="fhuE"
                     /locus_tag="OO244_000189"
                     /pseudo
     CDS             48388..50573
                     /gene="fhuE"
                     /locus_tag="OO244_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50633..52066)
                     /gene="ptsG"
                     /locus_tag="OO244_000190"
     CDS             complement(50633..52066)
                     /gene="ptsG"
                     /locus_tag="OO244_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5662163.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52361..53158)
                     /locus_tag="OO244_000191"
     CDS             complement(52361..53158)
                     /locus_tag="OO244_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5662164.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53169..54173)
                     /gene="holB"
                     /locus_tag="OO244_000192"
     CDS             complement(53169..54173)
                     /gene="holB"
                     /locus_tag="OO244_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5662165.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54170..54811)
                     /locus_tag="OO244_000193"
     CDS             complement(54170..54811)
                     /locus_tag="OO244_000193"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5662166.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54801..55823)
                     /gene="yceG"
                     /locus_tag="OO244_000194"
     CDS             complement(54801..55823)
                     /gene="yceG"
                     /locus_tag="OO244_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5662167.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55826..56635)
                     /gene="pabC"
                     /locus_tag="OO244_000195"
     CDS             complement(55826..56635)
                     /gene="pabC"
                     /locus_tag="OO244_000195"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5662168.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56755..57996)
                     /gene="fabF"
                     /locus_tag="OO244_000196"
     CDS             complement(56755..57996)
                     /gene="fabF"
                     /locus_tag="OO244_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5662169.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58083..58319)
                     /gene="acpP"
                     /locus_tag="OO244_000197"
     CDS             complement(58083..58319)
                     /gene="acpP"
                     /locus_tag="OO244_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5662170.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58530..59264)
                     /gene="fabG"
                     /locus_tag="OO244_000198"
     CDS             complement(58530..59264)
                     /gene="fabG"
                     /locus_tag="OO244_000198"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5662171.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59277..60206)
                     /gene="fabD"
                     /locus_tag="OO244_000199"
     CDS             complement(59277..60206)
                     /gene="fabD"
                     /locus_tag="OO244_000199"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5662172.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60222..61175)
                     /gene="fabH"
                     /locus_tag="OO244_000200"
     CDS             complement(60222..61175)
                     /gene="fabH"
                     /locus_tag="OO244_000200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5662173.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61243..62313)
                     /gene="plsX"
                     /locus_tag="OO244_000201"
     CDS             complement(61243..62313)
                     /gene="plsX"
                     /locus_tag="OO244_000201"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5662174.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62394..62567)
                     /gene="rpmF"
                     /locus_tag="OO244_000202"
     CDS             complement(62394..62567)
                     /gene="rpmF"
                     /locus_tag="OO244_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5662175.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62619..63140)
                     /gene="yceD"
                     /locus_tag="OO244_000203"
     CDS             complement(62619..63140)
                     /gene="yceD"
                     /locus_tag="OO244_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5662176.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63339..63923
                     /gene="yceF"
                     /locus_tag="OO244_000204"
     CDS             63339..63923
                     /gene="yceF"
                     /locus_tag="OO244_000204"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5662177.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64035..64994)
                     /gene="rluC"
                     /locus_tag="OO244_000205"
     CDS             complement(64035..64994)
                     /gene="rluC"
                     /locus_tag="OO244_000205"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5662178.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65567..68758
                     /gene="rne"
                     /locus_tag="OO244_000206"
     CDS             65567..68758
                     /gene="rne"
                     /locus_tag="OO244_000206"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5662179.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPIRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68955..69897)
                     /gene="flgL"
                     /locus_tag="OO244_000207"
                     /pseudo
     CDS             complement(68955..69897)
                     /gene="flgL"
                     /locus_tag="OO244_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69909..71551)
                     /gene="flgK"
                     /locus_tag="OO244_000208"
                     /pseudo
     CDS             complement(69909..71551)
                     /gene="flgK"
                     /locus_tag="OO244_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71617..72558)
                     /gene="flgJ"
                     /locus_tag="OO244_000209"
     CDS             complement(71617..72558)
                     /gene="flgJ"
                     /locus_tag="OO244_000209"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5662180.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72558..73655)
                     /gene="flgI"
                     /locus_tag="OO244_000210"
     CDS             complement(72558..73655)
                     /gene="flgI"
                     /locus_tag="OO244_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5662181.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLLQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73667..74365)
                     /gene="flgH"
                     /locus_tag="OO244_000211"
     CDS             complement(73667..74365)
                     /gene="flgH"
                     /locus_tag="OO244_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5662182.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74418..75200)
                     /gene="flgG"
                     /locus_tag="OO244_000212"
     CDS             complement(74418..75200)
                     /gene="flgG"
                     /locus_tag="OO244_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5662183.1"
                     /translation="MISSLWITKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75338..76089)
                     /gene="flgF"
                     /locus_tag="OO244_000213"
                     /pseudo
     CDS             complement(75338..76089)
                     /gene="flgF"
                     /locus_tag="OO244_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76109..77317)
                     /gene="flgE"
                     /locus_tag="OO244_000214"
     CDS             complement(76109..77317)
                     /gene="flgE"
                     /locus_tag="OO244_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5662184.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77342..78037)
                     /gene="flgD"
                     /locus_tag="OO244_000215"
     CDS             complement(77342..78037)
                     /gene="flgD"
                     /locus_tag="OO244_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5662185.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78049..78452)
                     /gene="flgC"
                     /locus_tag="OO244_000216"
                     /pseudo
     CDS             complement(78049..78452)
                     /gene="flgC"
                     /locus_tag="OO244_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78456..78872)
                     /gene="flgB"
                     /locus_tag="OO244_000217"
     CDS             complement(78456..78872)
                     /gene="flgB"
                     /locus_tag="OO244_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5662186.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79027..79686
                     /gene="flgA"
                     /locus_tag="OO244_000218"
     CDS             79027..79686
                     /gene="flgA"
                     /locus_tag="OO244_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5662187.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79762..80055
                     /gene="flgM"
                     /locus_tag="OO244_000219"
     CDS             79762..80055
                     /gene="flgM"
                     /locus_tag="OO244_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5662188.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80060..80476
                     /gene="flgN"
                     /locus_tag="OO244_000220"
     CDS             80060..80476
                     /gene="flgN"
                     /locus_tag="OO244_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5662189.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80516..82051)
                     /gene="murJ"
                     /locus_tag="OO244_000221"
     CDS             complement(80516..82051)
                     /gene="murJ"
                     /locus_tag="OO244_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5662190.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82251..83084)
                     /locus_tag="OO244_000222"
     CDS             complement(82251..83084)
                     /locus_tag="OO244_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5662191.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83086..83733)
                     /locus_tag="OO244_000223"
     CDS             complement(83086..83733)
                     /locus_tag="OO244_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5662192.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83744..84328)
                     /gene="rimJ"
                     /locus_tag="OO244_000224"
     CDS             complement(83744..84328)
                     /gene="rimJ"
                     /locus_tag="OO244_000224"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5662193.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84564..85772
                     /gene="mdtH"
                     /locus_tag="OO244_000225"
     CDS             84564..85772
                     /gene="mdtH"
                     /locus_tag="OO244_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5662194.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85836..86483
                     /gene="grxB"
                     /locus_tag="OO244_000226"
     CDS             85836..86483
                     /gene="grxB"
                     /locus_tag="OO244_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5662195.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86617..87177
                     /locus_tag="OO244_000227"
     CDS             86617..87177
                     /locus_tag="OO244_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5662196.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87283..88329
                     /gene="pyrC"
                     /locus_tag="OO244_000228"
     CDS             87283..88329
                     /gene="pyrC"
                     /locus_tag="OO244_000228"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5662197.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88403..88648
                     /gene="dinI"
                     /locus_tag="OO244_000229"
     CDS             88403..88648
                     /gene="dinI"
                     /locus_tag="OO244_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5662198.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88938..89192
                     /gene="bssS"
                     /locus_tag="OO244_000230"
     CDS             88938..89192
                     /gene="bssS"
                     /locus_tag="OO244_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5662199.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89307..90425
                     /gene="solA"
                     /locus_tag="OO244_000231"
     CDS             89307..90425
                     /gene="solA"
                     /locus_tag="OO244_000231"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5662200.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90473..90586
                     /locus_tag="OO244_000232"
     CDS             90473..90586
                     /locus_tag="OO244_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5662201.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90847..91413
                     /locus_tag="OO244_000233"
     CDS             90847..91413
                     /locus_tag="OO244_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5662202.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91417..91992
                     /locus_tag="OO244_000234"
     CDS             91417..91992
                     /locus_tag="OO244_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5662203.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92034..93086)
                     /locus_tag="OO244_000235"
     CDS             complement(92034..93086)
                     /locus_tag="OO244_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5662204.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93311..94231
                     /gene="lpxL"
                     /locus_tag="OO244_000236"
     CDS             93311..94231
                     /gene="lpxL"
                     /locus_tag="OO244_000236"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5662205.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94403..94600
                     /locus_tag="OO244_000237"
     CDS             94403..94600
                     /locus_tag="OO244_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5662206.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94624..>95534
                     /locus_tag="OO244_000238"
     CDS             94624..>95534
                     /locus_tag="OO244_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5662207.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        22980 a        25316 c        23578 g        23660 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc caaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatttt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagccccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtcccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gcgcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 gccaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacatgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtggccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt catacttcac gcattacgtt aagcatccgt tataatcggt
    22921 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
    22981 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
    23041 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
    23101 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
    23161 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
    23221 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
    23281 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
    23341 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
    23401 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
    23461 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
    23521 gctggaaaat gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat
    23581 tagcggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
    23641 ggatgttcca cctcgtgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
    23701 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
    23761 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
    23821 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
    23881 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
    23941 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
    24001 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
    24061 cgaagggtaa gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt
    24121 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
    24181 cttccagaat cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg
    24241 ggcaaggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
    24301 gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat
    24361 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc
    24421 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg
    24481 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
    24541 tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
    24601 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
    24661 ctttcgccgt gcccggatga acattgttac cgacaatttt tatattgacc gatgcggcgt
    24721 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
    24781 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
    24841 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
    24901 tgatttctgc aatacctgct ttgtcatcag cacctaacaa ggttttacca tcggtggtaa
    24961 tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
    25021 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
    25081 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
    25141 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
    25201 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
    25261 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
    25321 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
    25381 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
    25441 atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat
    25501 tcaagcggtt tgccgtagaa ttaccgccct cattaggagt caccaaggtg gttaaccaca
    25561 aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa
    25621 acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg
    25681 taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct
    25741 tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt
    25801 tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg
    25861 ctatgtgaac actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa
    25921 tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat
    25981 ggaagccctg cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc
    26041 tggtggtcaa cagcaacgcg tcgccattgc tcgagcggtg gttaacaagc ctcgtctgtt
    26101 gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga
    26161 gctgaaagcg ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga
    26221 agaagcactc accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga
    26281 cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg
    26341 cgaaattaat atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc
    26401 caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa
    26461 actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca
    26521 cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc
    26581 ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga
    26641 tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga
    26701 ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg
    26761 tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt
    26821 tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac actggataac tacacgcgtc
    26881 tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca
    26941 ccctcgcctg cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca
    27001 aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc
    27061 gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct
    27121 ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg
    27181 gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac
    27241 tggataagcc gttgcttgag gctgcgcgcg atctcggtgc cagcaagtta cagactttta
    27301 tccgcatcat tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc
    27361 tgccagcgat gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga
    27421 tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca
    27481 ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc
    27541 gtttgctgaa taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
    27601 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
    27661 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
    27721 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
    27781 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
    27841 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
    27901 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
    27961 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
    28021 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
    28081 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
    28141 aagctttacc ctgtcgatgg acgatgtggt agtttcttca ttcgtcaccg gaccaagtta
    28201 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
    28261 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
    28321 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
    28381 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
    28441 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
    28501 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc
    28561 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
    28621 gataaaatgc gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
    28681 agcaatctcg atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt
    28741 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
    28801 gtcaccagct gggccgatct gtggaagcca gagtacaaag gcagcctgct gttgaccgac
    28861 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
    28921 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca
    28981 gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
    29041 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg
    29101 ccgaaagaag gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
    29161 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
    29221 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa
    29281 gtggcaaacg ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
    29341 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
    29401 cgttaattca gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
    29461 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg
    29521 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac
    29581 ttttcaacaa attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    29641 ttaccaacct gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct
    29701 tcgtgtacaa acccagccgc cggatagaca tgcccggaag taccaatggc aataaagaca
    29761 tccgccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    29821 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    29881 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    29941 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    30001 gttaccagca aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg
    30061 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    30121 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    30181 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc
    30241 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    30301 aaaatacgct gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac
    30361 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    30421 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga
    30481 gctacaggta agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa
    30541 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    30601 agagaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    30661 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    30721 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    30781 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    30841 tggccaaact cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc
    30901 ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca
    30961 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    31021 tcaagacgtg cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg
    31081 gcatacagct taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    31141 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    31201 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    31261 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    31321 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    31381 gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    31441 gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataaa tatcgctgga
    31501 gaggaactga tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga
    31561 aacaactacg ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata
    31621 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    31681 atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac
    31741 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    31801 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    31861 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    31921 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    31981 gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg
    32041 ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg
    32101 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    32161 aaaattgcgc cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg
    32221 ttgtgggtta acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc
    32281 ggtgaaattg atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag
    32341 tgcttcctgc cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac
    32401 cgccagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    32461 caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat
    32521 gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc
    32581 cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt
    32641 tggcggctca tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc
    32701 tgcaagcgat tcaactcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    32761 ttaccggtag gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc
    32821 gctacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc
    32881 agccccaccg cttttaacat ctccagtgca cagctgttga tttcagcggg ctttttcttg
    32941 ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga
    33001 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt
    33061 ttgctcatcg gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct
    33121 aacaggtgca gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca
    33181 ccttcgccca cgctgaaact gacattgagc agcacatcgg tttgcacact gccttcctga
    33241 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    33301 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    33361 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    33421 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    33481 aagcgccgct ccgaggattg caccgataat cccggcgcta gccccttgca ccataaagac
    33541 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    33601 ctccatcacc atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag
    33661 gctcagcagt aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    33721 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    33781 aactctcagc ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg
    33841 catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    33901 ggcggcgaaa gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt
    33961 gaactggctg gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg
    34021 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    34081 cacattgacc agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac
    34141 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    34201 gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc
    34261 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    34321 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    34381 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc actgcacgcc cacgcatgta
    34441 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    34501 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    34561 agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatatt ttatcatcaa
    34621 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct
    34681 cgggtcataa ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg
    34741 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    34801 tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa
    34861 gtcctgagca aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt
    34921 tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    34981 tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag
    35041 tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc
    35101 tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc
    35161 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    35221 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    35281 cccacgccgt ggtggggaat gaacagtgtg atacgcaatt tgccttatta cagccttggc
    35341 gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    35401 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    35461 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta cgagcaacga
    35521 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    35581 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    35641 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg
    35701 ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat
    35761 ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcacc
    35821 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    35881 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    35941 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    36001 gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc
    36061 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    36121 gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    36181 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    36241 gcagcgtggc atactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag
    36301 ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt
    36361 acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga
    36421 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    36481 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    36541 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    36601 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    36661 ggaacatatt taccagcaag tgagtaaagg cacattacct gctgggatcg agtactggca
    36721 gccattgttc ttcagcgaac cactgccacc gctgttcagt tatttccctg ccaatacctt
    36781 gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc
    36841 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    36901 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgagtgc agctaaaaac
    36961 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    37021 ggccgttcag gcgcaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    37081 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    37141 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    37201 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg cgcgtaatct
    37261 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    37321 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    37381 gccggtggtc catctggagc acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc
    37441 tggcggcatt actggcgagt acctgatgct cacctatgcc aacgacgcca aactgtatgt
    37501 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    37561 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    37621 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    37681 cgcgtttaaa cacgatcgtg agcagtatca gttattctgc gacagcttcc cgtttgaaac
    37741 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    37801 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    37861 cgctttcctg gcagtagata accacaagca ggtagcggtg ttggtgccta ccacccttct
    37921 tgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    37981 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    38041 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    38101 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    38161 cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    38221 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    38281 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    38341 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    38401 cattcagaaa gccgccgaac ggctggcaga actggtgcct gaagcacgga ttgccatcgg
    38461 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    38521 tttcaacgtg ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa
    38581 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    38641 tcgcgtcgga cgttcacatc atcaggcata tgcatggctg ctgacgccgc atccaaaagc
    38701 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    38761 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    38821 agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    38881 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca
    38941 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcatcc ctgacgtgaa
    39001 cacgcgtctg tcgttctaca aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    39061 gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc gtaccctgct
    39121 ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    39181 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    39241 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    39301 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    39361 actggaagag aacgcgatcg cttaatgtaa atcgggggcg tttctgcgcc cccatctgca
    39421 acatttacaa attctttgca ctttcctgca ctatccgaca ctgtcaccat ccataattca
    39481 ggcttatctg tttattacaa taaccttata tttattatgt gtttttggtg atgatcaaaa
    39541 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    39601 cggcaaaagc caacacctgg ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    39661 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    39721 tcggctttct cgctctgtta caagctaacc ccggcgttga tccttacgta ccgcgcgcgg
    39781 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    39841 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    39901 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    39961 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    40021 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    40081 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    40141 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    40201 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    40261 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    40321 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    40381 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    40441 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    40501 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    40561 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    40621 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    40681 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    40741 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    40801 cgcagcgatg aggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg
    40861 caagaaatta cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga
    40921 tattttttat aacaatcatt aattaattta aaaatcttta aatttcagtc aaatagaaag
    40981 caattatttt tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt
    41041 cattcacagt caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa
    41101 aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat
    41161 tgttctggca acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcaaccggag
    41221 ccaaagcgcc cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc
    41281 tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga
    41341 aaagcgtgga gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca
    41401 aagacacccc ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag
    41461 gagatatagc caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt
    41521 ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg
    41581 cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc
    41641 tggaaaaact gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat
    41701 aagtcgcaaa aaaggcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc
    41761 ttgttatact ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc
    41821 cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata
    41881 accgagcatt ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta
    41941 agtatcgctt tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc
    42001 ccccacaaca gtggcgacat ctttaccgcg accaccacca aactgatgcc ctatcacgcc
    42061 accagcaaca gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg
    42121 atgggtcact gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg
    42181 ggttgcagaa acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac
    42241 tgccgctacg cccaacgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt
    42301 cacgggaaca cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc
    42361 tatcagacag aagatgaaaa gcgcgggaat gactggaaat acttaaggat tcggaggagt
    42421 ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt
    42481 tgtccagggc ggataaggcg tttataccac atccgccagt gtacgtcgat taatgcaact
    42541 tcaaacgcgg acggataacg cggttaatac tccccaccag catcattaat ccggttttaa
    42601 agtaaccatg cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa
    42661 ttcgtccttc aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg
    42721 agaagttcga cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct
    42781 taccgttcat ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct
    42841 gagcacgcgg cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag
    42901 cgtaaatgtc tggatcgcga gtggtttgca gcgtcggttc caccaccagc tggttgatac
    42961 ggttagtttc aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc
    43021 ataccatcag atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag
    43081 cactggtgac catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg
    43141 ccgcagcaga gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg
    43201 ttacgttcag ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgatcg
    43261 cgttgtgcaa ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt
    43321 tcactttgcc atttgcgccc aggttggcag agtatttcag gaacagattc agcatctcct
    43381 ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacgcctg
    43441 gcgtattgaa atcgttagag gtgctaccca gcgccatgac cagggtgtca taggcgattt
    43501 tacgttccgg aaccaacagt tcacctttct cgtcgcgtag ttctgcaata gtgattgttt
    43561 tcgcttcacg atcaatatca atgacggaac ccaactggaa ctggaaacca tgattgcgcg
    43621 catgggccag atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt
    43681 gcagcagtgg tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt
    43741 tcttacgtcc cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc
    43801 cgacaatcac aatctttttc aatggcgtag tcaacgtgcc ccccttaaat ttattaacca
    43861 attgttaatt aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa
    43921 tgttcttcgc tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa
    43981 tcaagttttg ttgcttaaaa gataggcaac tttaccgggg gagatcaaaa aatagagttt
    44041 attgtttatt atttgagcaa attcggaagg gtaatatagg gtgaagcgta tatcgagggc
    44101 gtttggtcgt cctgataaga tgcgcagcat cgcagcaggc attgatgctc gactgtcgga
    44161 tgcggcgtgg acgccttatc cgacttacag aattcggagc gcatcaggta ccggcaaggt
    44221 tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc gctgtaaatg
    44281 cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg
    44341 atgcaactct tcagatgtcc gctggctgtt aagtgcttca tcattacgcg acaaaataac
    44401 cagacaacga tcgcgattct tctcgcggaa gttggtcaca cacttagtgg caatatcggc
    44461 atactcttcc gggcgatcaa tcttgccttc catgttctca taagggaaca aattagggtt
    44521 gaagatcacc tggcggatat cgcaaagaaa accaatccgt tccgcccagt atccgcccag
    44581 gccaacgccg caaattagcg gacgctcgtc aacgttcagt tgcaacattt tgtccacttc
    44641 tttaagcaga tgctgcatat catgtttcgg atgccgcgta ctgtagctta tcaagcgtac
    44701 atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc
    44761 aaaaccgtgt aaatagataa tcatcgcttc ctcacataag ccagggttag tgacctgctt
    44821 tctcttcctg ccagcgttca tgtaactgat tcagacgggt gctgatcgct ttccagcgag
    44881 ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt
    44941 ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga
    45001 ccaggatcat atcgcaaccc gcatccagtg atgcctgccc gcgttcggca taactgccca
    45061 taatcgcggc accttccatc gataaatcgt cagagaaaat cacgccgtca aaccccagtt
    45121 cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc ggatcaacat
    45181 cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac
    45241 tgaagaccga catatcttta gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt
    45301 ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca
    45361 ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat
    45421 cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacatcc agcactggcg
    45481 caaagctgat atcaatatcc atagcgatca tttcgctggc catcagccaa cccgcctctt
    45541 gcgccagttt gccgccctct tccattccca acagcgcagc aaaggattgt gctgccggta
    45601 agcgggtaaa accttcgcga aaacgctgca cgcgtccacc ttcctgatct accgccacca
    45661 ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat
    45721 catgatagtt acgcgtaaag agaatcagcc ctcccaccag cggatgcgcc agtatttcac
    45781 gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc acactgctct
    45841 ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga
    45901 tcgccggttt gtcgccagcg gtactcaaac caccctcctt tgagcatcag cagccaggga
    45961 aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag
    46021 tcattgacca attgccggtg ctgttcagta ttttccaccc acaccgccgc cagttccagc
    46081 gcgatatcac catctccagc atactcccag tcgatgagtt ttaaccctga tgcgctatgc
    46141 actaaatttc cggcgtggac atccatatgc aatggactta agcgtaaagg tcgtggttcc
    46201 cgcgctttgc gcagacgttt taacattcgc agccaaccca cagtccgccg cgccggatcg
    46261 ctttgctgcc agtacagttc cagtaagggc aacagcgtta ttcgccagcc aaaacgtggt
    46321 tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt
    46381 accgcaccgg gcagatagtc gactaccatc cagtcacgga gatataaatg cggcttcggt
    46441 gcaatgcttg cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc
    46501 gcatcaggat cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa
    46561 ctaccgccac taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc
    46621 gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa acggcacctt tacctgacca
    46681 gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt
    46741 gccagaggcg caggagtaca gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc
    46801 tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct aactgttgct tagccatcga
    46861 cagttgctgg gcggaaacca gggtaaattt cccgttatta gccagcgcat ttcgcagcgt
    46921 ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag
    46981 caggacgcta cccgcagtca ccccgtcagc cccaagcatc ttactgacca tcggctgcat
    47041 tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgccgttc gatcttcgtg
    47101 ctcaattggg cctggctgct gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg
    47161 ttgtggctcg gctggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg
    47221 ttgccccaca cacccggcga gaaacatcgc cagcgcggta atcaaggcgt agcgactcat
    47281 ttttgtcatc aagattcacc cctttacaaa tatagataaa gtctgacttt gtgcgccccc
    47341 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
    47401 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
    47461 acggtaacgg gttcttgcct ttcgttataa atcgttgagg atgctgaagg ttgaatatca
    47521 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
    47581 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
    47641 aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
    47701 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
    47761 gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca ataccttctt
    47821 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
    47881 acacgtcgtt cacagtcgga atgagaatat tcggaatgat cagaatatgc gttggcgctt
    47941 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
    48001 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
    48061 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
    48121 gttaaacctg ctctttcaat cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
    48181 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
    48241 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttgca tttgcatttg
    48301 cagcaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg
    48361 acgacaacat aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa
    48421 tatcaagtca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
    48481 tctgccgctt ttgctgcacc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
    48541 gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg
    48601 cagatgactc aacatgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa
    48661 gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgatggggat cagcaaaagt
    48721 caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg
    48781 gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat
    48841 atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg
    48901 ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgacctgtcg tgaatttaaa
    48961 ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa
    49021 agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac
    49081 gactcatggc tggaccgcta caatagtgaa aagaccttct tctcgggcat tgtcgatgct
    49141 gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat
    49201 agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat
    49261 cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt
    49321 atgaccctga agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct
    49381 gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt
    49441 atgctggttg ggccatacag taattatgga cctggctttg atcatgtcgg cggcaccggt
    49501 tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa
    49561 ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt
    49621 tcagttcatg ggccaacatc ttcccggatg aaattggcag tttctacaac tttaatggca
    49681 atttcccaca agccgactgg tcatcacaga gcctggcgca ggacgatacc acacatatga
    49741 aatcgttata tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg
    49801 cacgttatac caactggcgg gttgatacgc tgacttacag catggagaaa aaccacacca
    49861 cgccttacgc tggtctggtg tttgacatca atgacaactg gtcgacctac gccagctata
    49921 cctctatttt ccagccgcaa aatgatcgtg acagttcagg caaatatctg gctccaatca
    49981 ccggtaacaa ctacgagctg ggtctgaaat cggactggat gaatagccgt ctgaccacca
    50041 cgttagccat cttccgtatt gagcaggata atgtcgctca gtccaccggt acacctatcc
    50101 ccggcagcaa cggcgaaacc gcctataaag cggtggatgg gacagtcagt aaaggggtgg
    50161 aatttgaact caacggcgca attaccgaca actggcagct gacatttggc gcaacgcgct
    50221 atcttgcaga ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc accacggtta
    50281 aaatgttcac cagctatcgg ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta
    50341 actggcaaaa tcgcgtgtat accgacaccg tgacaccgta tggcaccttc cgcgccgagc
    50401 aaggtagcta cgcgctggtg gatctcttca cccgctacca ggtgacgaaa aacttctcgt
    50461 tacaggggaa cgtcaataac ctgttcgaca aaacctacga taccaacatg gaaggttcta
    50521 tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac gtatcaattc tgaataacac
    50581 ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca acgtcttacg gattagtggt
    50641 tacggatgta ctcatccatc tcggttttca ggttatcgga tttagtaccg aaaatagcct
    50701 gaacaccaga accagcaacc actacgcccg ctgcgcccag tttcttcagg ccagcctgat
    50761 ccactttaga cacatcagca acgctgacgc gcagacgggt aatacatgcg tcgaggttag
    50821 taatgttttc tttaccacca aatgcagcaa ccagagccgg tgccatttcg ctggtacctg
    50881 ttgcttttgc atcttcagtc gcgtcttcac gacccggcgt tttcagatcc agtgctttaa
    50941 tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc gataccgacg atcggtaaca
    51001 gccacagttt gctgctgtta ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg
    51061 acgtaccgtc acgcatcccc agaagaatac agattgggaa tgccaggcct gccagaatcg
    51121 cgtggatgat gtacaggatc ggcgcaacga acatgaagaa gaactcgatc ggctcggtga
    51181 taccggtcag gaacgaggtc agcgccgcgg agatcataat accgcccact ttcgcgcggt
    51241 tttctggttt agcagagtgc cagatagcaa ttgcggcagc tggcagaccg tacattttga
    51301 acaggaagcc accagacagt ttacccgcag tcgggtcacc cgccatataa cgcggaatgt
    51361 cgccgtggaa aacctgacct gctgcgttgg tgtattcacc aatctgcatc tggaaaggta
    51421 cgttccagat gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc
    51481 caaacgcaac taccgggttc tggtaagcag cccactgaga gaaggtctgg attgcagaac
    51541 caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc aggccagaaa
    51601 tgatcggcac aaagcgttta ccggcaaaga agccaagata ctcaggcagc ttaatacggt
    51661 agaaacggtt aaacatgtac gctgcaatcg caccggagat aatccctccg agtacgccag
    51721 tatccgccaa gtgtttagag gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa
    51781 ccacggccat ggttttaacc atgatgccat aggcaacaac tgcggccagc gcggatacgc
    51841 catcgttatt ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa
    51901 agacggaacc gcctgcttct gccataacat gcgatacaac ggcgggcagc cagctgaaat
    51961 tcgcggaacc gacgcccagc agaatacctg cgataggtag tacggatacc ggcagcatca
    52021 gcgatttacc gaccttttgc aggttagcaa atgcattctt aaacataatt gagagtgctc
    52081 ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa ggggggagag cctcgccgtg
    52141 tacagggcat ctaagcgccc tttatttatt acacagagta aaataattca gtgccaattt
    52201 gtttgacggc tatcacgttt caacttcaga tcattggaga agttgcacat tttgacaaaa
    52261 catatgtcac aatatttcaa acgcggttat tcaccgccca attttcggcg gtgaacttta
    52321 ctcgttttag ccattaatta cgagctttaa aaaaactcat tcaacggatg gattgaaggc
    52381 gggaagcgtc gatgtgaaac agacgggcga agttatcggt ggttacctgc gccagttctt
    52441 caacggcaac acctttcaac acagccatgt attctgcaac gtcacgaacc atcgcaggtt
    52501 gattctcttt tcctcgatgc ggtaccggcg caaggtaagg tgagtcagtt tccaccagta
    52561 atcgatccag ggggacataa cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca
    52621 caatgccgga aaaggagatg taaaatccga gatccagtaa tttacccgcc gtttctctgt
    52681 cctctgtaaa acagtgtagt acgccaccgc aatccgtcac tttttcttcg cgcagaattg
    52741 ccagcgtatc ggcacgggcg tcacgggtat ggacgataac cggcttgttc agttcacgac
    52801 caatctggat atgatggatg aaggactctt gctgacgtac tttagtttcc ggtgtgtaat
    52861 aataatccag cccggtttca cccagcgcta caacaccctc ttctgccgcc agacggcgta
    52921 aatcttctac atcgtaggga tcattctggt tgagtggatg cacgccacaa gaaaatacga
    52981 cgttgtcacg ttcgcctacc agatcccgca tatgtaaata acccggtaat gttgtggcga
    53041 ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct
    53101 tatgcaaaga ttcataatcc agaccatcga gatggcagtg tgagtcgact aaaaacatga
    53161 tgtctctctt aaagatgagg aaccggtagc acaacgcccg gttgcaggta atgctcaata
    53221 cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc ctgtgacaga cattaactgt
    53281 tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc gcgagggaga aagatggttt
    53341 gccagttcgg cgaccaggcc cggcacatca acattggtca cctgcgcagc gccatgatgg
    53401 cgttttagcg catccatcag caacgttgcc agccagtgta aacgcgccgg agcttgttca
    53461 tgattaaggg ccgccagcag cgaataccag tcgcccgatg gcacgctata tgccaacgcc
    53521 tgacacaatg tttcacgagc ctgccagtta tctccctgaa acaacgccag tgccgcgcca
    53581 ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat cctgtgacat tgtcacttcg
    53641 cgtgaaagcc aagtcacggc gtactgttct ggaggcggcg caaggtaatg taaccgacaa
    53701 cgactacgta atgttgccag taaacgttca ggctcgcggg tagccaggaa aaaccaggtt
    53761 tctgctggtg gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag
    53821 gcagcatcgg ttacccagac aactttcgca ccacctaggc gtgcgtgctc attcagcttt
    53881 tcggtgacct cacgtaccgc atcaatgccc agcgtatttt tccctttttc gggagccagg
    53941 gtgtagtaat cgggatgcgt gccagcctgc atcaactgac atccacgaca gtgaccgcaa
    54001 cttttgtggc cctgcggttg ttggcagagt aaataacggc tcagggcgta gattaaagca
    54061 tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct tccggcctga
    54121 tagctggcta ccagtttttc gaaatcaggt cgtaaccatg gataccatct catgcgtcca
    54181 actccttcac ccagtgggtc acggtagtgc ggattgcatc catcacggcc tccagcggct
    54241 gggtggcatc aatggtatga atacttttat cttgtgcagc cagttccaga tagcgggcgc
    54301 gggtgcgatt aaagaaatcg aaagactctt gctcaatacg atccagctcg ccgcgcgcac
    54361 gcgcgcgttt taagccaact tccggggtaa catcgagata gagcgttaag tctgggcgaa
    54421 aatccccgag aacagcatca cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc
    54481 cgccctgata cgcctgagtg gaaagatcgt ggcgatcgcc aatcacccag gtgccgttag
    54541 ccagcgcagg tttgatgacc gtttctacca gttgaacgcg cgcggcataa aacatcagaa
    54601 cttcggcttt atcggtaatg acttcatcgc ctaccgattt gatatccagc accaggcttc
    54661 ttaacttttc ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg tcgcggatac
    54721 ccagttgctc gagcgtctca accaccacat tacgcgcggt agttttgcct gcgccttcca
    54781 gcccctcaat gacgatatac ttactgcgca tttttttcct taagcacttt cagataatcc
    54841 tgcacagact tgttatgact ggcaagattg gtattaaacg tgtgaccacc tttaccatcg
    54901 gccacaaaat agagatacgg cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc
    54961 cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc
    55021 ggcgtttcca ggtctgcacg agaaagtttg ccattataac gctctcccat cccgtaaatc
    55081 acggttgggt cggtctgcag gcgcatacca atgcgtaaac ggttgataaa taccgaggca
    55141 acctgatcgc gttcactggc aacggcggtt tctttttcga taattgatgc catcgtcacc
    55201 agctgatttt tatctttata aggcagaccg tccgcacgcc cttcccaggc gctatcgacc
    55261 gctttcacca ttttcttgtg aactcgcttg agtaacgcga catcggtggt attggcggta
    55321 tacatcaagg tgtctggcca gaaccaacct tcaatccact ccgggttttc cagttcaagt
    55381 gcctgcgcta cggtggcgta cttatcgtca ctcagcgtgt gcttgatata cggagcctca
    55441 cgcaattgct tgaggtagtc gctcagacgc atcccttcta ccagtcgcag cgggaactgt
    55501 gcttctttac cgctttccaa caatttcagc atctcgcgca cggtcatctg cggtgtaaag
    55561 cggtaagtcc cggctttaaa gtgagaaaga tccggttcga tacgcagcag ccattgaaaa
    55621 acccgtgggc gattgatgat cttatcagca taaagctgtt caccgagcgc cagacgtccg
    55681 gtccctggct tcagggtaaa tatcgtctct tctttgataa gcaatttgct gtcggcaaga
    55741 tggcgaacct tccagacgcc cacaccagcg gcgataccca gtaccaccag caataacaag
    55801 ataatcaata acactttttt catgactaat tcgggcgctc acaaagtggg gctaaatatt
    55861 catataacgt tgctgacgaa aaggtgacat cgccacaggc acgtacgggc atcactggca
    55921 ttaacgcatt acaaataacc atctcatctg cctgcaacgc ctcctccagc gaggcttgca
    55981 cttcgacaag ctgataagag gattgtgcca gtaaacggat acagaattgt cgcataatgc
    56041 cgttaacacc tgcctgatcc agtcgcggcg tataaactac attgcccttc cgccagaaca
    56101 aattagccgc acagcattcc gtaacccacc cttcgctgtc aaggaccagc gcctcatcag
    56161 cgtttgtctg ctcaagatga gagcgaatca atacttgctc aagccgatta agatgtttaa
    56221 tacctgcaag atgaggattg cgccccagcc gcaccgggct tagcgccaac gtcatccctt
    56281 cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg
    56341 gtccgctgtt caatgtgctg taccctcgcc cgccactacc gcgactgatc acgactttca
    56401 gcacaccatt ttgctgttct gctgccagcg ttttcatctc ttgttcaagc tgaggccaga
    56461 agtcacagga aatcatcaac cgctgacaag catcctgtag tcgcaggata tgcgccgata
    56521 acaaactgac tttaccgtcg ataactctgg cggtggtaaa acaaccatca ccaaactgcg
    56581 ttgcccgatc gcttactgcc agcgattcct gcttataacc gttaattaag aacataccgg
    56641 ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag tcggaataaa
    56701 agctaagaaa aaaggcccgc aagcggacct tttatatggg tggaaaatga caacttagat
    56761 ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac acagcgtgta
    56821 ttccattccg ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc
    56881 cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca ggatggagta
    56941 gatagattct actgcacccg ccgcacctaa caggtgaccg gtcatagatt tcgtggagct
    57001 taccaacaca cggcttgcag cttcaccgaa gatggttttc accgcctgcg cttcagcttt
    57061 atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat
    57121 gcctgcatca cgcagagcat ttgccattgc cagagctgcg cctgcgccat tttctggcgg
    57181 tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag cgtaaatttt
    57241 cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg caccatcgcc
    57301 cagtacgaag ccatcacgct ctttatccca cgggcggctc gccgcttgcg ggttatcatt
    57361 gcgggtagat aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg tactggcttt
    57421 ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac gcgcagcatg
    57481 gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg ggccacgcag
    57541 gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg gaacgaagaa
    57601 tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt cgatcagtcc
    57661 gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct cttccgttat
    57721 ttcaaggcca gaatcctgca tggcctgaac gccagcgaca attccatatt gaatgaaggc
    57781 atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat cctttactaa
    57841 gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc tgatgccact
    57901 ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag gagacaacat
    57961 gcccagtccg gtcacaacta cacgacgctt agacacgttt gtcctccaag gagggaaaaa
    58021 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    58081 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    58141 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    58201 gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc
    58261 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    58321 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    58381 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    58441 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    58501 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    58561 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    58621 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    58681 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    58741 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    58801 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    58861 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    58921 aaaacagatg aaaggttagt ttcgataata tcgttccact cttcatcttt cattcgcatt
    58981 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    59041 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    59101 cctttgccgt tggcacctaa ataatcactg atcgcctgag cgccattttc actggtcgca
    59161 gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    59221 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    59281 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    59341 acaatgcgtt tcgtcaggcc agtaagcacc ttgcccgggc cgacttcata gagatgttct
    59401 acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga
    59461 cgtaccagtg cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    59521 acaacagaaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    59581 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt
    59641 tttgcgccag cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca
    59701 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct
    59761 tcttcacacg ctttcgcaat aggcgcatca tccagaccga tgattgccgc catagcgccc
    59821 gtgccttccg gtacggcttc ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca
    59881 tcagcgaaat caatcacgcc agcgcaaacc agcgcggagt attcccccag gctgtgaccg
    59941 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat
    60001 gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc tgacccctgc
    60061 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    60121 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    60181 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgca
    60241 gagccccaag tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    60301 cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg
    60361 tggcgatcca gcgtcaccac gacattgtcc atcgacatac cgagtttttt cgccgttgca
    60421 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    60481 ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag
    60541 acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt
    60601 ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga aatgattccc
    60661 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    60721 ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    60781 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgca
    60841 caggctgctg caacgtcaaa tgccgggcaa cctttaatgt ccaacatgct ttgaatctga
    60901 caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc
    60961 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    61021 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    61081 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    61141 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    61201 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    61261 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    61321 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    61381 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    61441 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    61501 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    61561 agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc ctgtaaagcc
    61621 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    61681 ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcaagacc
    61741 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac
    61801 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc tgtcacaatc
    61861 gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt
    61921 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    61981 cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    62041 cagggccata cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc
    62101 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    62161 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    62221 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    62281 catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    62341 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    62401 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt
    62461 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    62521 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    62581 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    62641 aatacggcaa atgggtttgg cttttgcact tcttcaggca gttcaccaaa gaccatgtcc
    62701 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    62761 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    62821 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    62881 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    62941 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    63001 cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg
    63061 gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    63121 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    63181 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    63241 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    63301 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    63361 ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    63421 cacctgaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    63481 tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt
    63541 cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg
    63601 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    63661 tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc
    63721 gtcacctaag cgaggcggag attgataatt acgtgcgtaa ggagcatccc ctgcactgcg
    63781 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gagggacgtg
    63841 accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag
    63901 ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg catccggcaa
    63961 tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg
    64021 cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca
    64081 tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa cttcaacgct
    64141 gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat
    64201 tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg tgtatgcaca
    64261 cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc aaatgcatag
    64321 cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac acgcacgata
    64381 cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt cacatgcgac
    64441 tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc acgtaattgc
    64501 tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc
    64561 cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct
    64621 tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc
    64681 agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc
    64741 agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc
    64801 tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttca
    64861 cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa
    64921 aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga
    64981 gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc actcattttt
    65041 ctatgcttat atttagtttt gcaccttatt acttcactac gtgatcactt tattgatggt
    65101 tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta
    65161 aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc
    65221 cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag
    65281 caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta
    65341 ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct
    65401 gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc
    65461 gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact
    65521 ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt
    65581 aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta
    65641 tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa
    65701 aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca
    65761 cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca
    65821 tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga
    65881 taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag
    65941 ctatctggtt ctgatgccaa ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg
    66001 cgatgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg
    66061 gcttatcgtg cgcaccgctg gcgtaggcaa atctgctgag gcgctgcaat gggatttaag
    66121 cttccgcctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggctccgtt
    66181 cctgattcat caggagagca acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga
    66241 tatcggcgaa atccttatcg ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc
    66301 tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct
    66361 gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct
    66421 gccatccggt ggttccattg ttatcgacag caccgaagcg ttaacagcca tcgacatcaa
    66481 ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca ccaacctcga
    66541 agccgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat
    66601 cgacttcatc gacatgacgc caatacgcca ccagcgtgcg gtagaaaacc gtctgcgtga
    66661 agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct
    66721 ggaaatgtcc cgccagcgcc tgagcccatc actgggtgaa tccagccatc acgtctgccc
    66781 gcgctgctcc ggtaccggta ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg
    66841 tttgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt
    66901 gccaatcgct tcttatctgc tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg
    66961 tcaggacggt gttcgctgcg tgattgtgcc aaacgatcag atggaaaccc cgcactacca
    67021 cgtgttgcgc gtgcgcaaag gggaagaaac gccgacctta agctacatgc tgccgaagct
    67081 gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtcctga
    67141 acaacctgca ctggcaacct ttgccatgcc ggatgtaccg ccagcgccaa ccccagctga
    67201 acctgccgcg actgtcgtag ccccagcacc gaaagctgca acggcaacac cagcagctcc
    67261 tgcacaacct gggctgttaa gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg
    67321 tgaagaagcc aaaccgaccg agcaaccaac accgaaagca gaagcgaaac cggaacgtca
    67381 acaggatcgt cgcaaacctc gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac
    67441 ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca
    67501 ggcacagcag caaactgccg agacgcgtga gagccgtcag caggctgagg taacggaaaa
    67561 agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga
    67621 tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca
    67681 ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct
    67741 caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt
    67801 tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac gcacagaact
    67861 ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa
    67921 tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct
    67981 gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc
    68041 aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg
    68101 ctatccaatt gtacgtccgc aagatgtaca agttgaagag cagcgcgaac aggaagaagt
    68161 acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag
    68221 cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc
    68281 gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca
    68341 gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga
    68401 accggtggtt gaaccgcagg aagagacggc agatgcagat attgaagaag ttgccgaaac
    68461 tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc
    68521 tgaagtcgca gcagaagttg aaacggtggc cgcggttgaa ccagagatca ccgttgagca
    68581 taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc
    68641 gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg
    68701 tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata
    68761 attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc atgtttgaat
    68821 caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa
    68881 aaaatgcccg ccccggcgat cccagagcgg gcagacaaaa catacccagt ttcatgatat
    68941 gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat atcggtaaat
    69001 gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc
    69061 cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct
    69121 aaggaatcca gcgactccag tttgttcagc tgcgtgccta attccgcacg aacggtaagg
    69181 acattattca gcgagttttt cagtccacgg ttggttttat ccaatgccgc agcggcgatt
    69241 tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc
    69301 atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttactggta
    69361 atactgtcga aaattttgtc gcccgtatgt cctatcacca tcgaacgcga agcatcgacc
    69421 tgttgcttaa tactttctgt tccgccaaca taatcaccgt tagcctcact aaacggcgca
    69481 gtctctgttt tatatgtagc gcccattacc gtcagtggtg ttcgccagat tcagcaactg
    69541 gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc
    69601 attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag
    69661 tacactctct tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct
    69721 gttttgcgcc tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga
    69781 agggttaacg actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg
    69841 agaattggtg atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt
    69901 atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc
    69961 tgcgcattcg ccaggtaata ctgctgaaat cgttgcagat ttccgtactc ctcatcgaga
    70021 ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg
    70081 gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa
    70141 gcgtcgttaa aggatttcgc accgcccacc gttttactgt tgctttgcag atccagcagg
    70201 gcctgaccgt tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc
    70261 gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg
    70321 aagctgtcgt taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact
    70381 ttaccgttgg catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc
    70441 cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt
    70501 accgtggcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac cgcgggctta
    70561 ccgatagtaa agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg
    70621 ctgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc
    70681 agatcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc
    70741 gggatctcaa tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg
    70801 gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca
    70861 ttggcatcgt gatgttataa gtaccgccat cctgaacgct gacttctacg ccaacaatct
    70921 ggtttaattc gctcaccagt tgatcgcgtt gatccagcag attgttaggt gacgcccctg
    70981 cccccacgcc tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt ttagcgtaat
    71041 tgttgatctg atcaacgctg gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca
    71101 gatattgatc ggtggtttta aactgattca ccaacccttc tgatttccca atcagcgcct
    71161 ggcgcgccgc cgggtcttcc gcgttactca ccagcgtttg caggctggtg aagaaatcct
    71221 gcatctgtgt tgccagcgaa gaggtactgg tggagagcat attgtcgatt ttcgacatct
    71281 gctcatagcg ggcggtcagg ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa
    71341 taaacgtatc atactcacgc tgcacaccag aaacgtagac gccattgcca acccagccgc
    71401 cagcgcccaa cgtgctattg gcctgcgcca taatagtggt ttggcgggta tatccggcaa
    71461 cgttatagct ggagatatta ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc
    71521 cgctcatggc gttattgatc aagctggaca tggaggttcc ttataagcct tcaattacag
    71581 agtattatcg gcagcgaccc gccggacttg agttattcag aacagattat caatgttcat
    71641 gctgtaggtt ttgctcacct tgtcgcttat cgatttcatc tgctggatca tgttggtgag
    71701 tttacgggca tagtgaggat cggtagcata gcccgcgtcc tgtaggacct gcgccccctg
    71761 ttccgcactc gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta acagcccaac
    71821 gtaatccgac aatgcttcca gatacgagct gtagacccga aacttcgctt ttactttctt
    71881 cgcttcgccg ttttcatatt cagtcgtggt gatttcagtg acttgccctt tccagttgcc
    71941 agaggctttg acaccaaaca ggttatagct tggctcgccg ttttcgcggc ggatttgccg
    72001 ttgcccccaa ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct
    72061 ttgctggctt gccagttggg cgggcagcga gagttgcgcg aggaatgctc tactgtcacc
    72121 cggcagcgaa tcatcgtagt tacgtggtac ggccttttgc accagctgcg aaagcgtctg
    72181 gttttgataa cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc
    72241 ctctggcaat ggttgttctg gcgtcatctg tttaaccatc atctctgcaa gccccagacc
    72301 tttgcccgcc gtcatctgtt gggcaatttg ctggtcatac atactggtat acagacgagt
    72361 gtgctcgctg ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt tcaacatcat
    72421 ctgcacgaac atcccttcca cctgacgggc caccggacgg atatttgccg ccggatcttc
    72481 gctcgctttc gcctttagtt cgttgagtga ttgcgcatcc caggccgcac ttgccagtag
    72541 tttgctgtcg ctgatcatca gatgatttcc agttttgccc gcagacatcc cgcactttgc
    72601 attgattgca aaatagacat cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc
    72661 accacgttat tgaggctggc gctggaacgt acgctttgca gcgaaccgcc gctctggagt
    72721 aaatcgatct gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg tgtatctggc
    72781 tggctgacat tggcctgacg attaactgtt actgagagat ttccctgcgc taccgcgcag
    72841 ctgtcgaggg tcacttcgcg attcatcacc accgaaccgg tgcgcgagtt aatcactact
    72901 ttagcgtcct gcggggtgac attaacctgc atattctgga tatcggcaag gaaacggacc
    72961 tgggaactgt tgccacttgg tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg
    73021 ctgccatatc cacgcacgcg gttgatggtg tcagcgattt gctgcgccat gctgaaatct
    73081 tcgtcgttaa gttgcaaatt aagggtattc ccgacgccaa actggctggg caattcacgt
    73141 tcaataaccg caccattggt gatccgtcca ccgttcagtt ggttaacctg aacactgcta
    73201 ccgcctgcgg aggctcctgc gccgccaacc agaatattgc cctgcgccag cgcatacacc
    73261 tgactgtcaa cgcccttaag cggtgtcatc aacaacgtac ctccacgcaa gcttttggca
    73321 ttacccatgg aagaaaccac cacatcgatg gtttgcccct gacgtccaaa cggaggaagt
    73381 gacgctgtca ccattaccgc agcgacgttt tttagctgca tattggtgcc cgtcggaacg
    73441 gtaattccca gctgtgagag catgttatta agcgtttgtg tggtaaacgg cgtctgggtt
    73501 gtctggtcac cagtgccatc cagccccact accaagccat agccaatcag tgagttttgc
    73561 cttaccccct gaacactggt gagatcgcga atacgctcag cctgagccgc cgtcgtaacc
    73621 agtagaagaa ttaatgcaga gagaaattta atcaccacag cctcacttac attggcgaca
    73681 ggttaaggaa gaaacgctgc aaccagccca tattttgcgc ttcgttaatg tagccattgc
    73741 ctacgtattc aatacgcgca tccgccacct gagtagacgg tacggtattg ctgccgctga
    73801 tagtgcgtgg attaaccacg cccgagaagc gaataaattc ggtaccctga ttaatggcaa
    73861 tctgtttttc acccaccaca tgcaggttgc cgttgaccag tacctggtca accgtcaccg
    73921 tcaacgtgcc gctaaaggta ttgctggcat tggccccgcc ctttccgttg aacgtgttac
    73981 caccggaggc ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa tagcgcggca
    74041 cagtatcaaa gccaaaatta gttttaccgt cacggctggc attcgcagag gagcttttgc
    74101 tggcgctgac gttctcctgc aacacgatgg tcagcgtatc gccaatattg cgtggtcgac
    74161 gatcttcaaa cagcggttga tagccatagt taatcggctg agcagactgg aaaatagaac
    74221 cgttggcgac gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg
    74281 gcgtggaggg tatccaggcg cagccggtta gtgaaagcac caacaagctg gaaatggcat
    74341 aagtatgcgc agcgtttttt tgcatggcta tcatcttcaa aaatcagtaa accggtgaac
    74401 ctgccaccgg ttatgcctta gagttgcgtc aatttttgca gcatctgatc ggtggtggac
    74461 accgctttac tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc
    74521 gccacgttga cgttagacgt ttcaacatac ccttgataca gcagtcccgc gccgttcagg
    74581 cccggcgtgc tttcgttcgg tgcaccggag gattgcgttt cggtgtagag gttttcgcca
    74641 atgctctcca gtccggtatc attcataaag gtggtgagat tgagctgccc aacctgaacc
    74701 ggagctgcct ggccttgttg ggttacgctg accacgccat cacgaccgat ggtgatactt
    74761 aacgcattcg ccggaatggt gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc
    74821 tgcccgttct gatccacctg gaaagagccg tcacggatat aggctgatga gccatctggc
    74881 aacattacct ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt attggtctgc
    74941 gacagattgc cctggctgtg taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt
    75001 tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg cccccggctg gcgaatggtt
    75061 tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt actgacgttt
    75121 gccaggttgt tggcaatgac gtccatattg gtttgctggg cgtcaaggcc cgttttggtg
    75181 atccataatg aactgatcat ataatgtcct gtagtggatt gctgacaagc agagcagaag
    75241 cacgcgacag tgtgaatttc ccctcaccct aaccctctcc ccaaaggggc gagggaatag
    75301 tccgcgcgct tttattgcaa tgtcatcaat cgttcaatta actcatcgac agcagttggt
    75361 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    75421 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    75481 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    75541 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    75601 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    75661 ccgagattgt gccatcggca gcaatcgtga tttccgcccc ttccggcaca gcaattggcc
    75721 ccgcctcgcc tatcaccgga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    75781 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg
    75841 ctgcaacgca acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg tcatgtctgc
    75901 acccggtgtt gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac
    75961 tgcgcgcaag gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg ccagattact
    76021 ggcggttacc gcctgttgat tcagcgtctg gctggctgct cccatcgcag tataaattgc
    76081 gtgatccatt gagctatccc gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct
    76141 ggtcctgggt tttgatggtc tgggcgttag acttatagtt acgctgggca acgatcatat
    76201 tgaccagttc tttactgaga tcgacgttgg acgcttccag cgcaccgttg gtcagagtgc
    76261 caaagtttcc cgtcccggct gtccccaaca gcgccacgcc agaagattgc gtcgcagacc
    76321 agacgttgtc gccttcggat gccagacctt cgttgttggc aaagttcgcc agtacaatct
    76381 gccccagcag ttgggtttgt tcgttggaat tgttgccgac aaccgtaccg tcatcattga
    76441 tttgataact caccagatcg cccggtttgt agccattctg ggtggttgcc acaatgttgt
    76501 tagcgccggt attttgctgc atggagttga ggaagctcag actaaacgtg gcgggttctg
    76561 caccgttaat tgcgccggtt gcgatattat tcgccatcgc accatccact aatgtgccat
    76621 tagcattaaa ttccagtgtt gtcgctgtct tcgcaatgct gtttggatca ctgctatcct
    76681 gggtgtagac ctgccagtta ttatccccgg tcttcacaaa atagacgctc atgtcatgag
    76741 cattaccctg actgtcgaaa acagtcaccg aacctttttt gttatagcta tccgcattgc
    76801 tggcgctgaa tggcgtaaca gtaggaagcg gatcactgga attcaggttg atctgcatcg
    76861 acgccgtggt ggtagttttc gctgccatca gggtattcgg gatcgaaata ttggtcggat
    76921 tcgccccttg ctgaatagtc ggcggcgtac cggttaccgg gtaacccgtc agctgtaaac
    76981 cttgcgtatt caacaggtta cggttttcat ccagcttaaa ttgtccgtta cggctgtaga
    77041 acaccgaacc gttgctgtct accagacgga aaaaaccgtt ctggctgata gcaacgtcca
    77101 gacctcgccc ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt
    77161 ttacccccag tcccactttc gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt
    77221 taaagccgta ggtggcggag ttggcgatat tgttgccaat aacatcgagg ttggtggcgg
    77281 cagcgtttaa tccgctaacc gcttgagaaa aggccatgac tgactcctga aatgtgaagg
    77341 cttaaattat ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg
    77401 tattaccgct gttgccgcgg atcacaccct gcaccagagc aaactgcagc ggctgggcaa
    77461 ccagttgtgt accaccgtta ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag
    77521 tgctgccatc agtcaacgaa ccgtcccagg tgaaactgtg aactccggcg gtcagttcac
    77581 caatatcaat ggtgcgcaca accgcgccat ttttatcggt gatggtggcc gtaactttgt
    77641 ctgccgcctg ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac
    77701 tgccggttcc cgcaagaaca gttgtgccgg ggatcatcac gccgtgaccg atcaggttac
    77761 tggcctgtaa cgactggctg ttatcaatct gtccggaaat agatccgagc gtggtattga
    77821 gtttttcaat cccgctgacc gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt
    77881 ccattggatt ggtcgggtcc tggtttttca gctgcgccac cagcaaagtc agaaaactgc
    77941 tttgtaaatc tgcggcgttg ctgcccgtga gcgaactact gctggtggta ctgacgccgg
    78001 tatttgtcgg atcggtggtg gttaccgcaa tggacatagc tttctccttt attgaccgag
    78061 cgtaagggtt ttcagcatca tgcttttcac cgtgttgagc acttcaacat tggcctgata
    78121 gctgcgtgac gccgacatgg tgttaaccat ctccccgacg acatcaacgt tcggcatttt
    78181 tatgtagccc tttgcatctg ccagcggatt acctggttca taaaccagtt tgtccggggc
    78241 ctgactttct taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta
    78301 acctggaata ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca
    78361 gcatttgcca gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac
    78421 ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc
    78481 atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta
    78541 tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg
    78601 tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgc
    78661 tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg ttgcatgact
    78721 tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg
    78781 gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga
    78841 aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc
    78901 catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct
    78961 atttatgccg ttgatggtca ttgcggacag gtacaattca cgttgtagaa atggctgggg
    79021 gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta
    79081 agtacggcga gcaatctcac atcgcaattg cacaactttt ttagcgccca actcgcgggg
    79141 gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc gccatgcgag
    79201 cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca
    79261 cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg aaattatgtg
    79321 gttgccgcga tgcctattgt gcggggagga aagctggaag ctggcaatgt caaactgaaa
    79381 cgtggacgcc tggataccct gccaccgcgt acggtgctgg atatcaatca acttgttgat
    79441 gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag
    79501 gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc
    79561 gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc
    79621 atggtatcgg aacaggtagt cagcggcgtt gttgatgcag atgggaatat tcttataaac
    79681 ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc
    79741 gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa gcctgtaagc
    79801 accgttcaac cgcgcgaaac caccgacgcg ccggtaacga acagccgggc ggcaaaaaca
    79861 accgcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc
    79921 ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt
    79981 gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac
    80041 ttgcagagta actgatcgta tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt
    80101 gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta tggggcagat
    80161 caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg cgacgctgga
    80221 ttacctcgaa cagttacgca agaaagaacc caatacagca aatagcgttg atattagtca
    80281 acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc aacataacgg
    80341 ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca
    80401 tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaccccatc gcggcagtaa
    80461 aaagatttcg atctgaatat tatctccggc ctgcactgca ggccggaatg cattgttaca
    80521 ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg
    80581 cggcaatccc cgccagcacg accgccatta aacgcagtaa gcgccagggc atggtaccca
    80641 atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg
    80701 ccaccaccag acgcaacaga aacgccatcc agccgggttg cggagtaaag attttctgct
    80761 tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg
    80821 acagcccggc atgtttcaac ggaccaataa acgccaggtt catcaattgc gttaaaatca
    80881 gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag
    80941 ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct
    81001 gggtcatcag cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa acggttaacg
    81061 gacctgaaag aatgcccaac gcaaccgcac tcggcagtgc caacaggaaa caaagacgca
    81121 acccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca
    81181 gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accggaggga aactccatta
    81241 agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag gcaaaaatgg
    81301 tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggt cccatctgtt
    81361 tcaccacgcg cattgctcca gcatcgtgga agttaatgcg cggcaggacc agcatgccga
    81421 tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc gtaacagccc
    81481 acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca
    81541 tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga gaagcggttc cacgtattca
    81601 gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag gtaatcttta
    81661 gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca
    81721 tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc gcaagtgtca
    81781 gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat
    81841 attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgta
    81901 acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga
    81961 ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc
    82021 tgacggcggc cagcgatttt aataaattca tcggtgttct aatccggacc cacgggcaaa
    82081 acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctagattatg gtgaattgct
    82141 accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata
    82201 cgttgcgcca gcactgccta ttcgccggcg gtttgcggaa ctgtctgatt ttacacgcat
    82261 tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagagtgct ctgccaacca
    82321 ggaatcggtt tattcactac accctgcccg cgctcctcac gccattcgcg catatccgta
    82381 atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact tccggcacgg
    82441 cgatgcatac aagtggtgat ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc
    82501 atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccgcccgac
    82561 aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg
    82621 tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc ggtggcgagt
    82681 tgcgttttta actcaccgta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc
    82741 agttttttcc gcgccgccag ttccaccagc ctttcagcat cgcgcagatt ttctgccagc
    82801 ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac gtcaaagtgg
    82861 ctggcggtgc tggagtgcac aaaaaccgca tcacaactgg cggcaaggct ggataacgaa
    82921 tcggcataag gaatgcgcca gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac
    82981 caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca cgctttttgc
    83041 gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat ctcccagatg
    83101 ggccagcaac gaatcaagac gttgtttcag ttctgccact tcgatttcca gggcttcgac
    83161 gcgggtctgt aaatcaccgt caaccatgtt cgacatagcc gttaccgccg gctgatcttc
    83221 aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag
    83281 acgcaccaca aaaggaccat cttcgcgatt cgccagttgt tccagcgtcg actccacttc
    83341 cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg
    83401 ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca gtttcagatc
    83461 gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac caaaaccgct
    83521 cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca cttcggattc
    83581 actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga ctacaccatt
    83641 gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc aaccaattac
    83701 gcgagcttcc agggcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc
    83761 agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat
    83821 agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt
    83881 tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc tgcatataac
    83941 gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg
    84001 aataaccgag atagcaggca tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc
    84061 caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat
    84121 gaaattcgtt aatcatccct agcctggcct gccagcctga tggataacag tggctttcgt
    84181 cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgccagac
    84241 gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt
    84301 ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa
    84361 tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat
    84421 tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa
    84481 aatactgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca
    84541 tcttttattt cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat
    84601 atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga
    84661 tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt attgctctcg
    84721 gtctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt gccgaccgct
    84781 ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga ttcgccacaa
    84841 tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg ggactcggcg
    84901 gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt ccacaacaac
    84961 gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cagtgcggtc attggcgcat
    85021 tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggccgttt
    85081 tgtttgtgct atgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg
    85141 tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct
    85201 atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg atgctgccaa
    85261 ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa atggatgtat gccattgaag
    85321 cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc
    85381 tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg ccggtgggca
    85441 tggtcagcgg cctacaacaa cttttcaccc tgatttgtct gttttatatc gggtcgatca
    85501 ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga gctcgcggca
    85561 gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt tatatcggtg
    85621 gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc
    85681 tgggaattat tggcatcttc actttccttg cgctgggttg gcagtttagt cagaaacgcg
    85741 ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga ctcctccata
    85801 ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact
    85861 gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc gtcgaattac
    85921 atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc
    85981 ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc gttcactatg
    86041 tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc attgaagagt
    86101 ggctgcgcaa ggtcaatggc tacgtcaaca aactgctgtt gccgcgtttt gccaaatcgg
    86161 catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca
    86221 gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg
    86281 atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt
    86341 cggaagatga tattcagcta tttccgctac tgcgtaatct gacgctggta gccggaatca
    86401 actggccaag ccgcgttgct aattaccgcg ataatatggc gaaacagaca caaatcaatt
    86461 tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt cgcgggttgc
    86521 cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt
    86581 caggatattg acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct
    86641 gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc
    86701 gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tatcggttta
    86761 cccggcgtgg ctgacgccca tattgttctg acaaacttga ccagccaaat tggtcgcgaa
    86821 gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc cctgttcggt
    86881 agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa
    86941 ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg
    87001 caaacggtga tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa ttactttaac
    87061 cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa
    87121 ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac ggattaatga
    87181 cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt
    87241 gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc accatcccag
    87301 gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta
    87361 aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg
    87421 gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc
    87481 gtacctgccg ggcacgattt caccccattg atgacctgct atttaacaga ttcgctggat
    87541 cctaatgagc tggagcgcgg atttaatgaa gccgtgttca ccgctgcaaa actttacccg
    87601 gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat catgccggta
    87661 cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt gacacatgca
    87721 gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc
    87781 cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga tgctgccgac
    87841 tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca tctgatgttt
    87901 aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgttt acccatcctc
    87961 aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt taatcgagta
    88021 ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag ttgcggctgc
    88081 gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg
    88141 aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt ctatggcttg
    88201 ccggtcaacg acacattcat cgaactggta cgtgaagagc aacaggttgc tgaaagcatc
    88261 gcactgactg atgacaccct ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt
    88321 aaacaataaa aatcgtagct tcctgttgtc attaggttat tttacctgta taaataacca
    88381 gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt
    88441 gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt
    88501 tcctgataat gaaggccacg tatcggtacg ttatgccgca gcgaataatt tatcggttat
    88561 tggcgcaaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag
    88621 cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccggatcgcc
    88681 ccggcttttt tattttactt acgaattttg agtacaaaat ttctacactt cctcaaaata
    88741 attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt
    88801 aaaagcagaa atttatctgt tccccccttt aaaaattcgg tgaataccct tacttattgg
    88861 tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca
    88921 cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag
    88981 ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga
    89041 ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg
    89101 atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg
    89161 atttcccggt aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca
    89221 cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct
    89281 taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt
    89341 ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg
    89401 acgcccatat gccaccgcat caacacggca gccaccacgg cgatacgcga ttaattcgcc
    89461 atgcttatgg tgaaggcgaa aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg
    89521 atgaactttc ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc
    89581 tcggcccggc tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac
    89641 tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc
    89701 cggacaacta catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga
    89761 ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg
    89821 tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggttgac ggtgagtatc
    89881 aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc
    89941 ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc tatagcgtga
    90001 agaataaatt cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc
    90061 cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag
    90121 cggatgaacg tgttccgttt gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct
    90181 tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata
    90241 attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca
    90301 ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg
    90361 attttgcgca agaccaaaaa agcgattttg atttgacgcc attcaggctt tcccgcttcc
    90421 aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg
    90481 gctgttgcac tatctcatca ataatattcg cgagcatctg atgctttatc tttttctctg
    90541 gggattgttg gcaattatgg atctgatcta tgtgttttat ttttagtttt ccgcactgac
    90601 ataaactgac aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt
    90661 cctgcttcta cagcactcac cagacatctc tgatgctgaa cccactaaac ttatgaagat
    90721 tctttcatta ttaattcaaa attattgaaa taaaaataaa tatcattcaa tttgttattt
    90781 ttgtgttgca attcattctc cgacacgcca ttttctttct ctcgcaaatt cgtattggat
    90841 gcacttatgt cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat
    90901 tggctaagcg caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc
    90961 agttattacg atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg
    91021 ttaatgatgg ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg
    91081 ctgccgagtt attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtgc
    91141 ctattgctgt tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg
    91201 atcagcgttt ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca
    91261 gactttgccg gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtctgttatg
    91321 cacggattta tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg
    91381 ctgggaaagt cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta
    91441 accttcgcgt ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac
    91501 aaagaaggtc agcacgcctt tgttaacttc cgcatacagc atcttggcta tagctggtta
    91561 tatggcacct ttaaagattt cgacggtact tttacctttg atgaaaaaaa tccggctgcc
    91621 gataaagtga atgtgacaat taacaccacc agcgtcgata ctaaccacgc cgaacgcgat
    91681 aaacatcttc gcagtgcgga ttttctcaat accacaaaat atccacaggc aacattcacc
    91741 tccaccagcg tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat
    91801 ggcgtaacca aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg
    91861 ggcggtaaac gtgcaggctt tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc
    91921 aagacagatt taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta
    91981 cagcagaagt aagagtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct
    92041 gttggatcag gaatacccag tgttgtattc agacgtccac gagacttatt aaagatctta
    92101 ttgccatttt cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct
    92161 tcgcagcaaa tctcactaca acaacctttg tatttttccg cgcatactgg acactgaata
    92221 aacagcaggt ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac
    92281 tggtggcaat gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca
    92341 aaatttttgc caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca
    92401 ataattccgc cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg
    92461 gccttttcac aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc
    92521 tgcatcatct cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc
    92581 gcgttttcaa agtgccccac ttcatactca tagtggttac gcatgtcgat aaatagtgca
    92641 tcgggatcgt caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg
    92701 gcatcaaagt gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc
    92761 agtacccaga aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa
    92821 gccgggtcga aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg
    92881 atttgcgcgt taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta
    92941 aacagctgat ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa
    93001 aacgaaatgg tggttcgcgg ttcgctctca gccaacattt tggcttttaa cgcgtcgttg
    93061 gaaatgcggt tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa
    93121 tccgcggcat gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg
    93181 cgcattaaaa attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa
    93241 attttggttg cgggcgaaaa aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa
    93301 caggattgat atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg
    93361 gttaacctgg ttgggtattg gcgtactttg gttggtcgtg caattgccct acccgattat
    93421 ctaccgcctc ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa
    93481 aattgtgcat cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa
    93541 aatggtggtg aagaatttcg aatccgttgg catgggcctg atggaaaccg gtatggcgtg
    93601 gttctggccg gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg
    93661 tgacgtgcag gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga
    93721 gctgggcgcg cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga
    93781 taatccactg attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct
    93841 cgaccgcaaa gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta
    93901 cgcaccagat catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga
    93961 gcaggctgcg accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt
    94021 gcccttcgtt ccacgccgta agccagatgg caaagggtat caattgatta tgctgccacc
    94081 agagtgttct ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt
    94141 cgaaaaatgc atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac
    94201 acgcccggaa ggcgttcctt cacgctatta aatctcccat gccggatgct tcagaatggc
    94261 atccggcatt accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc
    94321 ccctggaagt cgggcgcata attagtgtgt ttatcttttc ttcttatgtt caccgcgcct
    94381 ggcgcaccaa cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa
    94441 acgaaacctg atcgtcgcct ggctaggctg ttttcttacc ggggccgcct tcagtctggt
    94501 aatgcccttc ttacccctct acgttgagca gcttggtgtt accggccact ccgccctgaa
    94561 tatgtggtcc ggtattgtct tcagtataat gggttcctaa acacatcatt ttaggtattg
    94621 aatatgagac gggcaatcag ctacatcaga ttttcaagtg aacgacaact gaaaggggac
    94681 tctgtacgcc gacaatctaa gctggttact gactggcttg ataagaaccc tgagttttac
    94741 ttggattcat ccttgagctt taaagatttg gggaaaagtg cttttagtgg taagcacctg
    94801 aaaggtggtc taggtgactt tttaaccgct atcgagaaag ggcttgttaa agctggtgat
    94861 acgctactaa tcgagagttt agaccgtctg tcacgtcagg atattgatat tgcttctgag
    94921 ctattacgcc gtatcttaag ggctggtgta gatgtagtca cactgagcga tggagaacat
    94981 tacacacgag aaagtttaaa agacccgtta gccctgatta aaagcattct gataatgcag
    95041 cgggcgcatg aggaaagttt gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa
    95101 aaagaactca tctccgaagg tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg
    95161 aatgatgacc gtcgaacatt cacaatcatt cctgataaag tggaagttgt taaacgagca
    95221 tttgacctga gattacaggg tttaagcttt tgggctatca ccagaacttt aaacgatgaa
    95281 ggtcacttgt cattgaatca atacacaccc aaacaaaagg ggtggtctga tactgctgta
    95341 aagaaactgc tcagaaatcg tgctgtgatt ggatgtttca cacctgctgg acgtgaagaa
    95401 gttcagggtt actaccccgc aataatcagt gagtcactat tctatcgagt acagcaactg
    95461 aacacagggc aatatggtag ggcttctgta tcaagtaatc cgctatcagt caacttgttc
    95521 aggggtatca tcaa
//
LOCUS       DAJTOW010000003        74373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTOW010000003 DAJTOW010000000
VERSION     DAJTOW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.003"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..1456
                     /gene="kdpA"
                     /locus_tag="OO244_000239"
     CDS             <1..1456
                     /gene="kdpA"
                     /locus_tag="OO244_000239"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5662208.1"
                     /translation="LAVLFFMLLGQHYLPLNPQQLPGLSWDLALNTAVSFVTNTNWQS
                     YSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSMSTLGNAWVDLLRITL
                     WVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGPVASQEAIKMLGTNGG
                     GFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGDRRQGRMLLWAMSVIF
                     VICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSLFAVVTTAASCGAVIA
                     MHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGRTPEYLGK
                     KIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSPHGFSEVLYAVSSAAN
                     NNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQPASSGTLPTH
                     GPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1479..3526
                     /gene="kdpB"
                     /locus_tag="OO244_000240"
                     /pseudo
     CDS             1479..3526
                     /gene="kdpB"
                     /locus_tag="OO244_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            3535..4107
                     /gene="kdpC"
                     /locus_tag="OO244_000241"
     CDS             3535..4107
                     /gene="kdpC"
                     /locus_tag="OO244_000241"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5662209.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4100..5083
                     /locus_tag="OO244_000242"
                     /pseudo
     CDS             4100..5083
                     /locus_tag="OO244_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            5083..6780
                     /locus_tag="OO244_000243"
                     /pseudo
     CDS             5083..6780
                     /locus_tag="OO244_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            6777..7462
                     /gene="kdpE"
                     /locus_tag="OO244_000244"
                     /pseudo
     CDS             6777..7462
                     /gene="kdpE"
                     /locus_tag="OO244_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            7737..7841
                     /locus_tag="OO244_000245"
     CDS             7737..7841
                     /locus_tag="OO244_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5662210.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8152..10349
                     /gene="speF"
                     /locus_tag="OO244_000246"
                     /pseudo
     CDS             8152..10349
                     /gene="speF"
                     /locus_tag="OO244_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            10346..11665
                     /gene="potE"
                     /locus_tag="OO244_000247"
     CDS             10346..11665
                     /gene="potE"
                     /locus_tag="OO244_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5662211.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(11721..13361)
                     /gene="pgm"
                     /locus_tag="OO244_000248"
     CDS             complement(11721..13361)
                     /gene="pgm"
                     /locus_tag="OO244_000248"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5662212.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(13387..13932)
                     /gene="seqA"
                     /locus_tag="OO244_000249"
     CDS             complement(13387..13932)
                     /gene="seqA"
                     /locus_tag="OO244_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5662213.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14117..14881
                     /gene="ybfF"
                     /locus_tag="OO244_000250"
     CDS             14117..14881
                     /gene="ybfF"
                     /locus_tag="OO244_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5662214.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15021..15314
                     /gene="ybfE"
                     /locus_tag="OO244_000251"
     CDS             15021..15314
                     /gene="ybfE"
                     /locus_tag="OO244_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5662215.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            15454..15984
                     /gene="fldA"
                     /locus_tag="OO244_000252"
     CDS             15454..15984
                     /gene="fldA"
                     /locus_tag="OO244_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5662216.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            16194..16280
                     /gene="uof"
                     /locus_tag="OO244_000253"
     CDS             16194..16280
                     /gene="uof"
                     /locus_tag="OO244_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5662217.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            16273..16719
                     /gene="fur"
                     /locus_tag="OO244_000254"
     CDS             16273..16719
                     /gene="fur"
                     /locus_tag="OO244_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5662218.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(16803..17129)
                     /locus_tag="OO244_000255"
     CDS             complement(16803..17129)
                     /locus_tag="OO244_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662219.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(17166..18572)
                     /gene="chiP"
                     /locus_tag="OO244_000256"
     CDS             complement(17166..18572)
                     /gene="chiP"
                     /locus_tag="OO244_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5662220.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(19151..20815)
                     /gene="glnS"
                     /locus_tag="OO244_000257"
     CDS             complement(19151..20815)
                     /gene="glnS"
                     /locus_tag="OO244_000257"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5662221.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21018..22964)
                     /gene="nagE"
                     /locus_tag="OO244_000258"
     CDS             complement(21018..22964)
                     /gene="nagE"
                     /locus_tag="OO244_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5662222.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            23297..24097
                     /gene="nagB"
                     /locus_tag="OO244_000259"
     CDS             23297..24097
                     /gene="nagB"
                     /locus_tag="OO244_000259"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5662223.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            24157..25305
                     /gene="nagA"
                     /locus_tag="OO244_000260"
     CDS             24157..25305
                     /gene="nagA"
                     /locus_tag="OO244_000260"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5662224.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            25314..26534
                     /gene="nagC"
                     /locus_tag="OO244_000261"
                     /gene_synonym="nagR"
     CDS             25314..26534
                     /gene="nagC"
                     /locus_tag="OO244_000261"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5662225.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            26582..27334
                     /gene="nagD"
                     /locus_tag="OO244_000262"
                     /pseudo
     CDS             26582..27334
                     /gene="nagD"
                     /locus_tag="OO244_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            27731..29395
                     /gene="asnB"
                     /locus_tag="OO244_000263"
     CDS             27731..29395
                     /gene="asnB"
                     /locus_tag="OO244_000263"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5662226.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            29774..29850
                     /locus_tag="OO244_000264"
     tRNA            29774..29850
                     /locus_tag="OO244_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29808..29810,aa:Met,seq:cat)
     gene            29859..29943
                     /locus_tag="OO244_000265"
     tRNA            29859..29943
                     /locus_tag="OO244_000265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29893..29895,aa:Leu,seq:tag)
     gene            29967..30041
                     /locus_tag="OO244_000266"
     tRNA            29967..30041
                     /locus_tag="OO244_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29999..30001,aa:Gln,seq:ttg)
     gene            30076..30150
                     /locus_tag="OO244_000267"
     tRNA            30076..30150
                     /locus_tag="OO244_000267"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30108..30110,aa:Gln,seq:ttg)
     gene            30166..30242
                     /locus_tag="OO244_000268"
     tRNA            30166..30242
                     /locus_tag="OO244_000268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30200..30202,aa:Met,seq:cat)
     gene            30291..30365
                     /locus_tag="OO244_000269"
     tRNA            30291..30365
                     /locus_tag="OO244_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30323..30325,aa:Gln,seq:ctg)
     gene            30403..30477
                     /locus_tag="OO244_000270"
     tRNA            30403..30477
                     /locus_tag="OO244_000270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30435..30437,aa:Gln,seq:ctg)
     gene            complement(30631..31806)
                     /gene="ubiF"
                     /locus_tag="OO244_000271"
     CDS             complement(30631..31806)
                     /gene="ubiF"
                     /locus_tag="OO244_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5662227.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            31952..33376
                     /gene="miaB"
                     /locus_tag="OO244_000272"
     CDS             31952..33376
                     /gene="miaB"
                     /locus_tag="OO244_000272"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5662228.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            33529..34569
                     /locus_tag="OO244_000273"
     CDS             33529..34569
                     /locus_tag="OO244_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5662229.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            34566..35033
                     /gene="ybeY"
                     /locus_tag="OO244_000274"
     CDS             34566..35033
                     /gene="ybeY"
                     /locus_tag="OO244_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5662230.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            35123..36001
                     /gene="corC"
                     /locus_tag="OO244_000275"
     CDS             35123..36001
                     /gene="corC"
                     /locus_tag="OO244_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5662231.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            36026..37564
                     /gene="lnt"
                     /locus_tag="OO244_000276"
     CDS             36026..37564
                     /gene="lnt"
                     /locus_tag="OO244_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5662232.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            37961..38869
                     /gene="gltI"
                     /locus_tag="OO244_000277"
     CDS             37961..38869
                     /gene="gltI"
                     /locus_tag="OO244_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5662233.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            39039..39779
                     /gene="gltJ"
                     /locus_tag="OO244_000278"
     CDS             39039..39779
                     /gene="gltJ"
                     /locus_tag="OO244_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5662234.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            39779..40453
                     /gene="gltK"
                     /locus_tag="OO244_000279"
     CDS             39779..40453
                     /gene="gltK"
                     /locus_tag="OO244_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5662235.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            40453..41178
                     /gene="gltL"
                     /locus_tag="OO244_000280"
     CDS             40453..41178
                     /gene="gltL"
                     /locus_tag="OO244_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5662236.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            41296..42231
                     /gene="rihA"
                     /locus_tag="OO244_000281"
     CDS             41296..42231
                     /gene="rihA"
                     /locus_tag="OO244_000281"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5662237.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            42315..43985
                     /gene="hscC"
                     /locus_tag="OO244_000282"
                     /pseudo
     CDS             42315..43985
                     /gene="hscC"
                     /locus_tag="OO244_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(44045..45496)
                     /gene="djlC"
                     /locus_tag="OO244_000283"
     CDS             complement(44045..45496)
                     /gene="djlC"
                     /locus_tag="OO244_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5662238.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSVWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(45493..46200)
                     /locus_tag="OO244_000284"
     CDS             complement(45493..46200)
                     /locus_tag="OO244_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5662239.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            46302..46856
                     /locus_tag="OO244_000285"
     CDS             46302..46856
                     /locus_tag="OO244_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5662240.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(46866..48293)
                     /locus_tag="OO244_000286"
     CDS             complement(46866..48293)
                     /locus_tag="OO244_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5662241.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(48290..48997)
                     /locus_tag="OO244_000287"
     CDS             complement(48290..48997)
                     /locus_tag="OO244_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5662242.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            49161..50138
                     /locus_tag="OO244_000288"
     CDS             49161..50138
                     /locus_tag="OO244_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5662243.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(50208..50690)
                     /locus_tag="OO244_000289"
     CDS             complement(50208..50690)
                     /locus_tag="OO244_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5662244.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            50925..53507
                     /gene="leuS"
                     /locus_tag="OO244_000290"
     CDS             50925..53507
                     /gene="leuS"
                     /locus_tag="OO244_000290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5662245.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KGGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            53522..54103
                     /gene="lptE"
                     /locus_tag="OO244_000291"
     CDS             53522..54103
                     /gene="lptE"
                     /locus_tag="OO244_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5662246.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            54103..55134
                     /gene="holA"
                     /locus_tag="OO244_000292"
     CDS             54103..55134
                     /gene="holA"
                     /locus_tag="OO244_000292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5662247.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            55136..55777
                     /gene="nadD"
                     /locus_tag="OO244_000293"
     CDS             55136..55777
                     /gene="nadD"
                     /locus_tag="OO244_000293"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5662248.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            55801..56412
                     /locus_tag="OO244_000294"
     CDS             55801..56412
                     /locus_tag="OO244_000294"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5662249.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            56672..56989
                     /gene="rsfS"
                     /locus_tag="OO244_000295"
                     /gene_synonym="rsfA"
     CDS             56672..56989
                     /gene="rsfS"
                     /locus_tag="OO244_000295"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5662250.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            56993..57460
                     /gene="rlmH"
                     /locus_tag="OO244_000296"
     CDS             56993..57460
                     /gene="rlmH"
                     /locus_tag="OO244_000296"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5662251.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            57491..59392
                     /gene="mrdA"
                     /locus_tag="OO244_000297"
     CDS             57491..59392
                     /gene="mrdA"
                     /locus_tag="OO244_000297"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5662252.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            59395..60507
                     /gene="mrdB"
                     /locus_tag="OO244_000298"
                     /gene_synonym="rodA"
     CDS             59395..60507
                     /gene="mrdB"
                     /locus_tag="OO244_000298"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5662253.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            60518..61606
                     /gene="rlpA"
                     /locus_tag="OO244_000299"
     CDS             60518..61606
                     /gene="rlpA"
                     /locus_tag="OO244_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5662254.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            61745..62956
                     /gene="dacA"
                     /locus_tag="OO244_000300"
     CDS             61745..62956
                     /gene="dacA"
                     /locus_tag="OO244_000300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5662255.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            63067..63330
                     /locus_tag="OO244_000301"
     CDS             63067..63330
                     /locus_tag="OO244_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5662256.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            63431..64072
                     /gene="lipB"
                     /locus_tag="OO244_000302"
     CDS             63431..64072
                     /gene="lipB"
                     /locus_tag="OO244_000302"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5662257.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            64331..65284
                     /locus_tag="OO244_000303"
     CDS             64331..65284
                     /locus_tag="OO244_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5662258.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            65493..66458
                     /gene="lipA"
                     /locus_tag="OO244_000304"
     CDS             65493..66458
                     /gene="lipA"
                     /locus_tag="OO244_000304"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5662259.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(66559..66762)
                     /gene="tatE"
                     /locus_tag="OO244_000305"
     CDS             complement(66559..66762)
                     /gene="tatE"
                     /locus_tag="OO244_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5662260.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(66891..67678)
                     /locus_tag="OO244_000306"
                     /pseudo
     CDS             complement(66891..67678)
                     /locus_tag="OO244_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
     gene            67771..68154
                     /gene="crcB"
                     /locus_tag="OO244_000307"
     CDS             67771..68154
                     /gene="crcB"
                     /locus_tag="OO244_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5662261.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(68208..68417)
                     /gene="cspE"
                     /locus_tag="OO244_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(68208..68417)
                     /gene="cspE"
                     /locus_tag="OO244_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5662262.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(68592..69152)
                     /gene="pagP"
                     /locus_tag="OO244_000309"
     CDS             complement(68592..69152)
                     /gene="pagP"
                     /locus_tag="OO244_000309"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5662263.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            69741..71126
                     /gene="dcuC"
                     /locus_tag="OO244_000310"
     CDS             69741..71126
                     /gene="dcuC"
                     /locus_tag="OO244_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5662264.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLTSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(71167..71508)
                     /locus_tag="OO244_000311"
                     /pseudo
     CDS             complement(71167..71508)
                     /locus_tag="OO244_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(71846..72355)
                     /locus_tag="OO244_000312"
     CDS             complement(71846..72355)
                     /locus_tag="OO244_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5662265.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEESVAPLL
                     EPQDAKKAV"
     gene            72518..72763
                     /locus_tag="OO244_000313"
     CDS             72518..72763
                     /locus_tag="OO244_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5662266.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            72738..74369
                     /locus_tag="OO244_000314"
                     /pseudo
     CDS             72738..74369
                     /locus_tag="OO244_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18289 a        18732 c        18929 g        18423 t
ORIGIN      
        1 gctggcggta ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
       61 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
      121 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
      181 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
      241 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
      301 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
      361 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
      421 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
      481 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
      541 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
      601 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
      661 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
      721 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
      781 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
      841 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
      901 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
      961 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     1021 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     1081 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     1141 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     1201 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     1261 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     1321 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     1381 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     1441 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     1501 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     1561 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     1621 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     1681 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     1741 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     1801 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     1861 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     1921 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     1981 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     2041 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     2101 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     2161 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     2221 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     2281 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     2341 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     2401 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     2461 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     2521 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     2581 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     2641 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     2701 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     2761 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     2821 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     2881 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     2941 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     3001 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     3061 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     3121 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     3181 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     3241 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     3301 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     3361 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     3421 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     3481 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     3541 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     3601 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     3661 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     3721 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     3781 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     3841 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     3901 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     3961 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     4021 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     4081 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     4141 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     4201 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     4261 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     4321 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     4381 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     4441 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     4501 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     4561 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     4621 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     4681 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     4741 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     4801 gcgcgcctgg agggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     4861 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     4921 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     4981 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     5041 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     5101 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     5161 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     5221 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     5281 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     5341 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     5401 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     5461 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     5521 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     5581 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     5641 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     5701 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     5761 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     5821 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     5881 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     5941 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     6001 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     6061 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     6121 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     6181 gaaggatcac cccacgcccg tcaggccagc gagattcgtc agcatgtgct gaacactacc
     6241 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     6301 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     6361 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga cctaaatcca cgttgacggg
     6421 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     6481 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     6541 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     6601 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     6661 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     6721 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     6781 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     6841 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     6901 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     6961 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     7021 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     7081 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     7141 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     7201 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     7261 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     7321 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     7381 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     7441 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     7501 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     7561 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     7621 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     7681 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     7741 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     7801 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     7861 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     7921 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     7981 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     8041 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     8101 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     8161 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     8221 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     8281 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     8341 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     8401 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     8461 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     8521 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     8581 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     8641 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     8701 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
     8761 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
     8821 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
     8881 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
     8941 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
     9001 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
     9061 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
     9121 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
     9181 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
     9241 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
     9301 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
     9361 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
     9421 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
     9481 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
     9541 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
     9601 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
     9661 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
     9721 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
     9781 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
     9841 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
     9901 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
     9961 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    10021 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    10081 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    10141 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    10201 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    10261 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    10321 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    10381 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    10441 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    10501 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    10561 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    10621 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    10681 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    10741 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    10801 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    10861 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    10921 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    10981 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    11041 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    11101 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    11161 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    11221 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    11281 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    11341 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    11401 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    11461 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    11521 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    11581 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    11641 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    11701 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    11761 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    11821 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    11881 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    11941 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    12001 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    12061 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    12121 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    12181 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    12241 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    12301 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    12361 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    12421 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    12481 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    12541 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    12601 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    12661 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    12721 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    12781 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccctgtct
    12841 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    12901 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    12961 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    13021 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    13081 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    13141 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    13201 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    13261 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    13321 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    13381 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    13441 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    13501 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    13561 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    13621 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    13681 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    13741 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    13801 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    13861 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    13921 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    13981 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    14041 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    14101 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    14161 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    14221 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    14281 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    14341 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    14401 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    14461 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    14521 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    14581 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    14641 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    14701 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    14761 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    14821 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    14881 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    14941 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    15001 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    15061 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    15121 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    15181 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    15241 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    15301 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    15361 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    15421 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    15481 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    15541 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    15601 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    15661 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    15721 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    15781 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    15841 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    15901 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    15961 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    16021 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctgta
    16081 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    16141 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    16201 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    16261 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    16321 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    16381 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    16441 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    16501 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    16561 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    16621 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    16681 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    16741 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    16801 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    16861 tgatcatttc cggtttgcgt tgctcgcaga cactgctgat aatccagtac gcgtacactt
    16921 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    16981 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    17041 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    17101 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    17161 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    17221 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    17281 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    17341 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    17401 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    17461 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    17521 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    17581 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    17641 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    17701 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    17761 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    17821 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    17881 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    17941 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    18001 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    18061 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    18121 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    18181 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    18241 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    18301 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    18361 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    18421 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    18481 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    18541 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    18601 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    18661 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    18721 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    18781 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    18841 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    18901 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    18961 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    19021 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    19081 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    19141 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    19201 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    19261 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    19321 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    19381 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    19441 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    19501 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    19561 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    19621 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    19681 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    19741 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    19801 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    19861 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    19921 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    19981 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    20041 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    20101 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    20161 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    20221 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    20281 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    20341 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    20401 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    20461 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    20521 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    20581 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    20641 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    20701 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    20761 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    20821 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    20881 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    20941 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    21001 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    21061 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    21121 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    21181 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    21241 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    21301 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    21361 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    21421 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    21481 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    21541 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    21601 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    21661 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    21721 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    21781 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    21841 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    21901 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    21961 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    22021 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    22081 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    22141 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    22201 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    22261 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    22321 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    22381 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    22441 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    22501 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    22561 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    22621 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    22681 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    22741 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    22801 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    22861 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    22921 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    22981 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    23041 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    23101 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    23161 tttactggct aaaccagaaa acttatttta tcattcaaaa aattaagtcg gattgacgcc
    23221 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    23281 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    23341 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    23401 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    23461 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    23521 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    23581 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    23641 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    23701 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    23761 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    23821 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    23881 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    23941 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    24001 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    24061 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    24121 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    24181 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    24241 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    24301 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    24361 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    24421 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    24481 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    24541 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    24601 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    24661 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    24721 attgtggttt ctgcaggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    24781 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    24841 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    24901 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    24961 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    25021 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    25081 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    25141 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    25201 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    25261 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    25321 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    25381 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    25441 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    25501 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    25561 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    25621 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    25681 agacgctgga acatgccctg ctgaatgcta ttgctcagtt tattgatagc taccagcgca
    25741 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    25801 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    25861 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    25921 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    25981 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    26041 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    26101 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    26161 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    26221 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    26281 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    26341 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    26401 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    26461 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    26521 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    26581 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    26641 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    26701 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    26761 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    26821 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    26881 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    26941 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    27001 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    27061 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    27121 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    27181 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    27241 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    27301 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    27361 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    27421 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    27481 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    27541 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    27601 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    27661 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    27721 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    27781 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    27841 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    27901 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    27961 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    28021 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    28081 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    28141 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    28201 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    28261 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    28321 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    28381 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    28441 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    28501 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    28561 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    28621 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    28681 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    28741 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    28801 ttatctttac ttccataaag cgcccaacgc taaagaactg catgaagaga cggtgcgtaa
    28861 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    28921 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    28981 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    29041 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    29101 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    29161 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    29221 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    29281 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    29341 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    29401 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    29461 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    29521 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    29581 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    29641 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    29701 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    29761 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    29821 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    29881 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    29941 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    30001 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    30061 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    30121 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    30181 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    30241 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    30301 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    30361 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    30421 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    30481 taaaaaagct cgcatcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    30541 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    30601 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    30661 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    30721 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    30781 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    30841 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    30901 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    30961 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    31021 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    31081 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    31141 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    31201 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    31261 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    31321 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    31381 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    31441 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    31501 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    31561 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    31621 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    31681 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    31741 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    31801 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    31861 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    31921 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    31981 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    32041 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    32101 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    32161 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    32221 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    32281 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    32341 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    32401 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    32461 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    32521 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    32581 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    32641 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    32701 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    32761 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    32821 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    32881 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    32941 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    33001 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    33061 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    33121 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    33181 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    33241 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    33301 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    33361 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    33421 atccctatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    33481 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    33541 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    33601 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    33661 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    33721 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    33781 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    33841 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    33901 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    33961 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    34021 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    34081 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    34141 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    34201 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    34261 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    34321 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    34381 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    34441 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    34501 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    34561 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    34621 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    34681 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    34741 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    34801 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    34861 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    34921 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    34981 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    35041 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    35101 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    35161 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    35221 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    35281 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    35341 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    35401 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    35461 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    35521 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    35581 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    35641 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    35701 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    35761 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    35821 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    35881 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    35941 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    36001 aaaccgaaac tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat
    36061 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    36121 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    36181 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    36241 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    36301 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    36361 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    36421 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    36481 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    36541 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    36601 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    36661 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    36721 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    36781 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    36841 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    36901 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    36961 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    37021 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    37081 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    37141 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    37201 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    37261 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    37321 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    37381 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    37441 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    37501 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    37561 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    37621 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    37681 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    37741 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    37801 cgcaaaaaca aacaataaaa catcaatatc tttacattta accaaactaa atgttaccta
    37861 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    37921 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    37981 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    38041 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    38101 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    38161 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    38221 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    38281 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    38341 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    38401 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    38461 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    38521 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    38581 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    38641 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    38701 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    38761 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    38821 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    38881 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    38941 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    39001 agttaacgct gcaaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    39061 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    39121 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    39181 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    39241 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    39301 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    39361 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    39421 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    39481 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    39541 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    39601 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    39661 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    39721 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    39781 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    39841 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    39901 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    39961 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    40021 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    40081 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    40141 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    40201 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    40261 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    40321 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    40381 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    40441 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    40501 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    40561 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    40621 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    40681 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    40741 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    40801 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    40861 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    40921 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    40981 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    41041 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    41101 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    41161 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    41221 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    41281 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    41341 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    41401 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    41461 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    41521 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    41581 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    41641 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    41701 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    41761 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    41821 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    41881 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    41941 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    42001 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    42061 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    42121 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    42181 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    42241 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    42301 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    42361 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    42421 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    42481 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    42541 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    42601 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    42661 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    42721 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    42781 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    42841 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    42901 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    42961 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    43021 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    43081 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    43141 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    43201 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    43261 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    43321 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    43381 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    43441 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    43501 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    43561 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    43621 atcagtcgat tgatattctc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    43681 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    43741 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    43801 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    43861 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    43921 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    43981 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    44041 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    44101 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    44161 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    44221 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    44281 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    44341 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    44401 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    44461 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    44521 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    44581 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    44641 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    44701 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    44761 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    44821 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    44881 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    44941 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    45001 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    45061 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    45121 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    45181 ccatgttcca ggtatttaat tgctgaataa atttctgcca gacggagggc tgaaaacgat
    45241 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    45301 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    45361 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    45421 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    45481 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    45541 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    45601 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    45661 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    45721 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    45781 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    45841 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    45901 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    45961 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    46021 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    46081 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    46141 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    46201 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    46261 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    46321 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    46381 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    46441 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    46501 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    46561 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    46621 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    46681 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    46741 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    46801 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    46861 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    46921 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    46981 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    47041 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    47101 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    47161 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    47221 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    47281 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    47341 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    47401 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    47461 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    47521 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    47581 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    47641 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    47701 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    47761 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    47821 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    47881 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    47941 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    48001 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    48061 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    48121 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    48181 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    48241 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    48301 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    48361 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    48421 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    48481 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    48541 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    48601 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    48661 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    48721 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    48781 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    48841 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    48901 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    48961 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    49021 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    49081 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    49141 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    49201 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    49261 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    49321 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    49381 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    49441 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    49501 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    49561 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    49621 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    49681 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    49741 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    49801 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    49861 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    49921 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    49981 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    50041 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    50101 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    50161 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    50221 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    50281 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    50341 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    50401 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    50461 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    50521 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    50581 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    50641 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    50701 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    50761 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    50821 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    50881 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    50941 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    51001 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    51061 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    51121 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    51181 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    51241 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    51301 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    51361 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    51421 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    51481 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    51541 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    51601 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    51661 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    51721 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    51781 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    51841 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    51901 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    51961 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    52021 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    52081 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    52141 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    52201 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    52261 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    52321 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    52381 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    52441 cagagtacaa aggaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    52501 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    52561 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    52621 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    52681 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    52741 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    52801 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    52861 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    52921 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    52981 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    53041 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    53101 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    53161 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    53221 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    53281 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    53341 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    53401 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    53461 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    53521 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    53581 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    53641 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    53701 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    53761 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    53821 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    53881 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    53941 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    54001 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    54061 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    54121 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    54181 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    54241 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    54301 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    54361 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    54421 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    54481 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    54541 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    54601 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    54661 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    54721 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    54781 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    54841 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    54901 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    54961 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    55021 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    55081 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    55141 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    55201 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    55261 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    55321 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    55381 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    55441 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    55501 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    55561 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    55621 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    55681 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    55741 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    55801 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    55861 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    55921 ctgctagatg atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat
    55981 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    56041 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    56101 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    56161 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    56221 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    56281 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    56341 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    56401 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    56461 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    56521 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    56581 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    56641 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    56701 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    56761 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    56821 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    56881 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    56941 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    57001 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    57061 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    57121 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    57181 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    57241 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    57301 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    57361 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    57421 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    57481 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    57541 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    57601 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    57661 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    57721 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    57781 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    57841 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    57901 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    57961 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    58021 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    58081 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    58141 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    58201 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    58261 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    58321 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    58381 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    58441 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    58501 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    58561 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    58621 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    58681 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    58741 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    58801 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    58861 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    58921 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    58981 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    59041 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    59101 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    59161 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    59221 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    59281 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    59341 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    59401 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    59461 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    59521 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    59581 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    59641 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    59701 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    59761 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    59821 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    59881 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    59941 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    60001 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    60061 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    60121 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    60181 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    60241 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    60301 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    60361 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    60421 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    60481 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    60541 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    60601 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    60661 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    60721 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    60781 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    60841 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    60901 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    60961 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    61021 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    61081 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    61141 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgactctaa aaagcgaaga
    61201 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    61261 tggtgtactg gaaggcagcg aaccgacacc tgcgccacag cccgttgtta cagcttcgtc
    61321 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    61381 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    61441 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    61501 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    61561 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    61621 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    61681 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    61741 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    61801 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    61861 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    61921 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    61981 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    62041 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    62101 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    62161 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    62221 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    62281 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    62341 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    62401 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    62461 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    62521 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    62581 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    62641 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    62701 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    62761 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    62821 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    62881 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    62941 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    63001 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    63061 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    63121 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    63181 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    63241 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    63301 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    63361 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    63421 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    63481 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    63541 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    63601 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    63661 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    63721 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    63781 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    63841 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    63901 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    63961 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    64021 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    64081 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    64141 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    64201 aactcacccc attgattaac ctattgaaat agttgattat ggtgagcaaa agcgttccgt
    64261 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    64321 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    64381 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    64441 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgccccc
    64501 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    64561 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    64621 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    64681 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    64741 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    64801 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    64861 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    64921 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    64981 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    65041 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    65101 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    65161 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    65221 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    65281 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    65341 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    65401 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    65461 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    65521 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    65581 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    65641 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    65701 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    65761 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    65821 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    65881 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    65941 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    66001 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    66061 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    66121 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    66181 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    66241 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    66301 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    66361 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    66421 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    66481 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    66541 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    66601 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    66661 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    66721 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    66781 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    66841 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    66901 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    66961 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    67021 acgccaaagg gatctataat ccggctttgc ccgatatttt gttcccgcac tcccccgccg
    67081 ccaccatata acaggtggta tccagcgcac gagcggcaag caacgttgac cagtgatgct
    67141 ctttgagcgg cccacgaacc caggcggcag gaagtaccag gatttcagct ccctgtaaag
    67201 cctgtgccag cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca
    67261 tcccttccac ctccagtaac ggagctattt cattaccagc atcaacatgg cgtgattcct
    67321 gaatggcaaa tgcatcatag agatgcagtt tggcataacg ggcgacgatg tttcctgcct
    67381 gaagtgccac cagcatattc catgcgcgcc ccggcgttga aggaagatga atcgtcagaa
    67441 ttgtcgtcat catgttacgt ttactttctc gccgtaaacg tccgaggaat tcgccttcca
    67501 gcagctgtgc tgatttaacc gatagatctg catcatgatc atcgcgcgcc agcaatgctt
    67561 ccggcaggac aaacagcgat acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa
    67621 tctcagcgtt cttttcccac acagatgtaa cagcaaactg tcctgcagca accagcattg
    67681 ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt acacttatca
    67741 ctcatacaaa tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt
    67801 ggcggtggta cgggaagcgt agcgagatgg ctgttaagta tgcgatttaa cccgctgcat
    67861 caggcgattc cgttggggac gctggcagca aacctgattg gggcattcat cataggaatg
    67921 ggattcgcct ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc
    67981 accggatttt gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg
    68041 ttacaagagg gccgctttgg ctgggcatta ctgaacgttt tcgtcaacct tctggggtct
    68101 tttgccatga ccgcactggc attctggctg ttttcggcct caaccgtaca ctaaagacca
    68161 aaaaaaaccc gctgattaag cgggttttga attcttgctg acgtatctta cagagcgatt
    68221 acgtttgcag cagaagggcc tttggcaccg ttagtgattt cgaactctac gcgctgacct
    68281 tcagcaagag ttttaaaacc attagtctgg attgcagaga agtgtacgaa cacgtctttg
    68341 ctgccgtctt ccggagtaat gaaaccgaat cctttggact cattaaacca cttaacgtta
    68401 cctttaatct tagacatcaa aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg
    68461 gttatcagta caccaattgt ggtacgcttt gtccagtgca actttactaa aaagtgatag
    68521 aagtcgcgtt atttttgatg ttgcttattt tagttattga ttttaaagaa gttactaaaa
    68581 cttcatttgt ctcaaaactg aaagcgcatc caggcaaagt acacattgcc attgttgtag
    68641 gtacccggaa tgtaggtcat ctgaaaagtc actgggccat aacccacgga ggccaatggc
    68701 agtagaaccg ggagagggat gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat
    68761 cccagaccta aatgaaaatt ttcatccgcc agcggtcgcc aggtactttc ccatccgtat
    68821 ccggcaatcg gttcccattt gttccacgag tccttaaatg ccatggcata caggccatgc
    68881 cagtttcctt tttcatccca acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta
    68941 tagcgatcgg ttttttcttt gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata
    69001 tataaatcat aatgttcagg ctgttgccag gtttgtgcaa tattttctct aaacgttgtc
    69061 atcctctcat ctgcgttagc aaaaacttta ccaacgctga ttaactgaat aaaaacaaag
    69121 gaaaagatag cgacatattt actcacgttc atttgtgacc ataaaacatt tatcaaaaat
    69181 ctactactag catagcaaag ctacaattaa cataacctta atagacccaa cataaagaat
    69241 aatctgaata gctgctctat ttatttccgc ttgttcaagt taaatatttc cgcgcatcga
    69301 ttaaagatga tgcgtcgttt tctgcgatgg gaatagtcaa aaaagaaaaa ccaagtcttt
    69361 tttgatgaca aaatgcaatc aaggaaaaat tattttattt ttttaacgag atacaacaat
    69421 catcttaacg aagtatataa aatatatcgt caacccatgc tcatattaga ttattttttg
    69481 agcgcacagc cgcagctaac accattgcaa ttaacaaatt tgcatcaatc caccatcaat
    69541 ttacacacat tattatgtga taattgccaa ccgctaaata tgcgttttgt tatctatgta
    69601 taaaaacagc aacttcaatg tcttaatggc agtttttctt gattttaatc agcattcatc
    69661 gccaatttat tgggcatatt ttttccttaa gctttaggaa gtttttatta tttactttgg
    69721 gcctggagac aggaaaaatt atgctgacat tcattgaact ccttattggg gttgtggtta
    69781 ttgtgggtgt ggctcgctac atcattaaag ggtattccgc cactggtgtg ttatttgtcg
    69841 gtggcctgtt attgctgatt atcagtgcca ttatggggca caaagtgtta acgtccagcc
    69901 aggcttcaac aggctacagc gccacggata tcgttgaata cgttaaaata ttactaatga
    69961 gccgcggcgg cgacctcggc atgatgatta tgatgctgtg tggctttgcc gcttacatga
    70021 cccatatcgg cgcgaatgat atggtggtca agctggcgtc aaaaccattg cagtatatta
    70081 actcccctta cctgctgatg attgccgcct attttgttgc ttgtctgatg tcactggccg
    70141 tctcttccgc aaccggtctg ggtgttttgc tgatggcaac cctgtttccg gtgatggtaa
    70201 acgttggtat cagtcgtggc gcagcagctg ccatttgtgc ctccccggcg gcgattattc
    70261 tcgcaccgac ttcaggggat gtggtgctgg cggcgcaggc ttccgaaatg tcgctgattg
    70321 acttcgcctt caaaacgacg ctgcctatct caattgctgc aattatcggc atggcgatcg
    70381 cccacttctt ctggcaacgt tatctggata aaaaagagca catctctcat gaaatgttag
    70441 atgtcagtga aatcaccacc actgcccccg cgttttatgc cattttgccg ttcacgccga
    70501 tcatcggtgt actgattttt gacggtaaat ggggtccgca attacacatc atcactattc
    70561 tggtgatttg tatgctgatt gcctccattc tggagttcct ccgcagcttt aatacccaga
    70621 aagttttctc tggtctggaa gtggcttatc gcgggatggc agatgcgttt gctaacgtgg
    70681 tgatgctgct ggttgccgct ggggtattcg ctcaggggct tagcaccatc ggctttattc
    70741 aaagtctgat ttctatcgct acctcgtttg gttcggcgag tatcatcctg atgctggtat
    70801 tggtgattct gaccatgctg gcggcagtca cgaccggttc aggcaatgcg ccgttttatg
    70861 cgtttgttga gatgatcccg aaactggcgc actcttccgg cattaacccg gcgtatttga
    70921 ctatcccgat gctgcaggcg tcaaacctgg gtcgtaccct atcacccgtt tctggcgtag
    70981 tcgttgcggt tgccgggatg gcgaaaatct caccatttga agtcgtaaaa cgcacctcgg
    71041 tgccggtgct tgttgggcta gtgattgtta tcgttgctac agagctgatg gtgccaggaa
    71101 cggcagcagc ggtcacaggc aagtaaatag taatgccgga ggcaagtttc ctccggcttt
    71161 atcatgtcac ccactgtggt atatgcgttg tggtctgcca actttgccgt gaacaatttc
    71221 agcaataatc agatggcggc tggcgcaata ttcaagataa cgcctggcag tggtgcggct
    71281 gatggtcagt gcctgcgcca ccgtttctgc cgtatgttgc acaccaggct ctttaaacag
    71341 ttttcgcacc gcgtttagcg tcaaggggtc aatgccggtc ggcagttcgt ctttgggttc
    71401 accgcgagca taggcattaa acatctcatc aatttgcttc tggctggcgc tatcaatact
    71461 ttccagcata tgtttacgct ggcggaaacg ggttagcgtt tgccccagtg tattgattca
    71521 cttgaagtac gaaaaaaccg ggagaacatt ggattattcg ggatctgatg ggattagatt
    71581 tggtggggct tgcaagcctg tagtacaaat tttaggcgtt aaccaatgaa acgcgaaaga
    71641 tagtaaaaaa ttgcttttgt ttcattgaaa aaacgaaaaa caaaaacact ataaatcatt
    71701 tcaatcatag cttcaaaaaa cgttcaaaac cgataacaac caggctgtca ccaaatgact
    71761 catatcacaa atcagctcat gccgtttagg tatgttacat gtgtgattat gtgaggtgaa
    71821 gtatgtttta gctggctcat ggttgttata cggctttttt cgcatcttgt ggttccaaca
    71881 aaggagctac agactcttcc tgtttatgaa caccatacat tgacaaaatc tcttggcgag
    71941 tttctggagg taatttatgc agcgccaaag ctgtttgtag tgtttggcga tcaataccca
    72001 gcgttgccaa tagggtgtca acggagatgg aggggcgttc ttgcattaat ccggaaagcc
    72061 catatttagc catgtttagc tctatctctc ggacttcatt ttctcctagt ttattgatta
    72121 acagggagaa cattttcaat gtatcagcat tcatttcttc gatagcaatt ttttgcatag
    72181 ccggtattgc cgggatatat tcaaccccag gcccgcgacg cccttcttgt gaacgcttcg
    72241 gccatccttc tttctttgct cgtttatgca ccccctgtat tgtcccaggc attccatcca
    72301 gttcagtaat ttgtttgggt gtcatccact ggtttatctc atttgcgctt ttcataaact
    72361 aagtttacca gatttatcta tataaaaatc aatggattac aaaataaacc acattaactg
    72421 atataaaccg gtttggttta tttggtaatg tataaaacag ataatcaaga cgaattattt
    72481 gttttggtaa ccctttatca tacgcaatat ggttggtgtg cgaaagatga gtaacgattg
    72541 gcatcgggcc gacgtgattg cggccctcaa aaaaacagga acccctctgg caaaattgtc
    72601 cagacgttca ggttattcaa ccgggacgct tgcaaatgca ttagtcaggc catggcctaa
    72661 aggtgaacgc attattgctg acgccttaaa catgaaacct gaggaaattt ggcctagtag
    72721 atatctaagg gatcacaatg agagagtgga tatcagcaat tgaatgcatg ggtttatctg
    72781 gtttacctgg ttcattaatg ggggttcaca aaagagctca gagagaagga tggctcaaga
    72841 gaagaagaga aggaattaga ggggttgctt acgaatacca tatatcttct cttcctaaca
    72901 ttgctcgagc tgaagttttg ctccaacaag gaaaatttaa aaccagtcag ggatgttttg
    72961 aaatcgcccg ccccacgctg gaagctcatg attatgatcg tgaggcacta tggagcaaat
    73021 gggataaagc cagcgattcc cagcgcagcc tggctgaaaa atggttgccc tcaattcagg
    73081 ccacagacga aatgctgaac caggggattt caacgaaaac ggcttttgcg accgttgcag
    73141 ggcattacca ggtcagcgca tccactttgc gggacaagta ttaccaggta cagaagtttg
    73201 cgaagcctga ctgggcggct gcacttgttg atggacgtgg agcatcccgt cgcaatgttc
    73261 acaaaagtga atttgacgag gatgcctggc agtttctgat tgcagattat ctgcgaccgg
    73321 aaaaacccgc cttccgcaaa tgttatgagc gtctggaact ggcagcccgc gagcatggct
    73381 ggagtattcc ctcccgtgcc acggcctttc gccggattca gcaactggac gaggcaatgg
    73441 ttgttgcctg tcgtgaaggt gaacatgcac tgatgcatct gataccggca cagcagcgaa
    73501 ctgtggaaca cctggacgcc atgcagtgga tcaacggcga cggttatctg cataacgtct
    73561 ttgtacgctg gtttaacggt gatgtgatcc gtccgaaaac atggttctgg caggatgtga
    73621 aaacccgaaa aattctgggc tggcgctgcg atgtgagcga gaacattgat tcaattcgcc
    73681 tctcgttcat ggatgttgtg actcgctaca gtatcccgga ggattttcac atcaccattg
    73741 ataacacccg tggtgctgcg aataaatggc tgacgggagg cgcgcccaat cgctaccgct
    73801 ttaaggtaaa agaggacgat ccaaaaggac tgtttttact gatgggtgcg aaaatgcact
    73861 ggacaagcgt tgttgccggt aaaggctggg gccaggcaaa acctgttgaa cgtgctttcg
    73921 gtgttggtgg gcttgaggaa tacgttgata agcatccggc actggctggc gcatatacgg
    73981 ggccaaatcc gcaggcaaaa cctgataact atggcgacca cgctgttgat gcagagctgt
    74041 ttctgaaaac ctttgccgaa ggtgtggcga tgttcaatgc cagaacaggc cgtgaaacag
    74101 aaatgtgcgg gggcaaactc tcgtttgatg atgttttcga gcgtgaatac gccagaacga
    74161 ttgtgcgtaa gccaaccgaa gaacaaaaac ggatgctgtt actgcctgcc gaggcggtga
    74221 acgtttcacg caaaggcgag tttacgctta aagttggcgg ctcccttaaa ggcgcgaaaa
    74281 acgtttatta caacatggca ttaatgaatg ccggagtgaa aaaagttgtg gtcagatttg
    74341 atccacagca gttacacagc acggtttatt gag
//
LOCUS       DAJTOW010000004        72932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTOW010000004 DAJTOW010000000
VERSION     DAJTOW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.004"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..247)
                     /gene="tuf"
                     /locus_tag="OO244_000315"
     CDS             complement(<1..247)
                     /gene="tuf"
                     /locus_tag="OO244_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5662267.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDC"
     gene            complement(318..2432)
                     /gene="fusA"
                     /locus_tag="OO244_000316"
     CDS             complement(318..2432)
                     /gene="fusA"
                     /locus_tag="OO244_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5662268.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2529..2999)
                     /gene="rpsG"
                     /locus_tag="OO244_000317"
     CDS             complement(2529..2999)
                     /gene="rpsG"
                     /locus_tag="OO244_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5662269.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3096..3470)
                     /gene="rpsL"
                     /locus_tag="OO244_000318"
     CDS             complement(3096..3470)
                     /gene="rpsL"
                     /locus_tag="OO244_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5662270.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3596..3883)
                     /gene="tusB"
                     /locus_tag="OO244_000319"
     CDS             complement(3596..3883)
                     /gene="tusB"
                     /locus_tag="OO244_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5662271.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3891..4250)
                     /gene="tusC"
                     /locus_tag="OO244_000320"
     CDS             complement(3891..4250)
                     /gene="tusC"
                     /locus_tag="OO244_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5662272.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4250..4636)
                     /gene="tusD"
                     /locus_tag="OO244_000321"
     CDS             complement(4250..4636)
                     /gene="tusD"
                     /locus_tag="OO244_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5662273.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4636..5358)
                     /locus_tag="OO244_000322"
     CDS             complement(4636..5358)
                     /locus_tag="OO244_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5662274.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5525..6337)
                     /gene="fkpA"
                     /locus_tag="OO244_000323"
     CDS             complement(5525..6337)
                     /gene="fkpA"
                     /locus_tag="OO244_000323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5662275.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6558..6776
                     /locus_tag="OO244_000324"
     CDS             6558..6776
                     /locus_tag="OO244_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5662276.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6825..7415)
                     /gene="slyD"
                     /locus_tag="OO244_000325"
     CDS             complement(6825..7415)
                     /gene="slyD"
                     /locus_tag="OO244_000325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5662277.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7510..7710)
                     /locus_tag="OO244_000326"
     CDS             complement(7510..7710)
                     /locus_tag="OO244_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5662278.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7720..9457)
                     /gene="kefB"
                     /locus_tag="OO244_000327"
                     /pseudo
     CDS             complement(7720..9457)
                     /gene="kefB"
                     /locus_tag="OO244_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9457..10008)
                     /gene="kefG"
                     /locus_tag="OO244_000328"
     CDS             complement(9457..10008)
                     /gene="kefG"
                     /locus_tag="OO244_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5662279.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10139..12052
                     /locus_tag="OO244_000329"
     CDS             10139..12052
                     /locus_tag="OO244_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5662280.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12052..13074
                     /locus_tag="OO244_000330"
     CDS             12052..13074
                     /locus_tag="OO244_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5662281.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13068..13286
                     /locus_tag="OO244_000331"
     CDS             13068..13286
                     /locus_tag="OO244_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5662282.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13340..14209
                     /locus_tag="OO244_000332"
     CDS             13340..14209
                     /locus_tag="OO244_000332"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5662283.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14264..14668)
                     /locus_tag="OO244_000333"
     CDS             complement(14264..14668)
                     /locus_tag="OO244_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5662284.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14970..15602
                     /gene="crp"
                     /locus_tag="OO244_000334"
     CDS             14970..15602
                     /gene="crp"
                     /locus_tag="OO244_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5662285.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15653..17743
                     /locus_tag="OO244_000335"
     CDS             15653..17743
                     /locus_tag="OO244_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5662286.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17810..19030)
                     /gene="argD/dapC"
                     /locus_tag="OO244_000336"
     CDS             complement(17810..19030)
                     /gene="argD/dapC"
                     /locus_tag="OO244_000336"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5662287.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19116..19679)
                     /gene="pabA"
                     /locus_tag="OO244_000337"
     CDS             complement(19116..19679)
                     /gene="pabA"
                     /locus_tag="OO244_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5662288.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19711..20313)
                     /locus_tag="OO244_000338"
     CDS             complement(19711..20313)
                     /locus_tag="OO244_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5662289.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20303..20470)
                     /locus_tag="OO244_000339"
     CDS             complement(20303..20470)
                     /locus_tag="OO244_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5662290.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20575..21156)
                     /gene="ppiA"
                     /locus_tag="OO244_000340"
                     /gene_synonym="rotA"
     CDS             complement(20575..21156)
                     /gene="ppiA"
                     /locus_tag="OO244_000340"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5662291.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21427..22608
                     /gene="tsgA"
                     /locus_tag="OO244_000341"
     CDS             21427..22608
                     /gene="tsgA"
                     /locus_tag="OO244_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5662292.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVVGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22870..25413
                     /gene="nirB"
                     /locus_tag="OO244_000342"
     CDS             22870..25413
                     /gene="nirB"
                     /locus_tag="OO244_000342"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5662293.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25410..25736
                     /gene="nirD"
                     /locus_tag="OO244_000343"
     CDS             25410..25736
                     /gene="nirD"
                     /locus_tag="OO244_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5662294.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25862..26668
                     /gene="nirC"
                     /locus_tag="OO244_000344"
     CDS             25862..26668
                     /gene="nirC"
                     /locus_tag="OO244_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5662295.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26687..28060
                     /gene="cobA"
                     /locus_tag="OO244_000345"
     CDS             26687..28060
                     /gene="cobA"
                     /locus_tag="OO244_000345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5662296.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28307..28474
                     /locus_tag="OO244_000346"
     CDS             28307..28474
                     /locus_tag="OO244_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5662297.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28769..30106
                     /gene="frlA"
                     /locus_tag="OO244_000347"
                     /pseudo
     CDS             28769..30106
                     /gene="frlA"
                     /locus_tag="OO244_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30127..31149
                     /gene="frlB"
                     /locus_tag="OO244_000348"
     CDS             30127..31149
                     /gene="frlB"
                     /locus_tag="OO244_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5662298.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTTKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31199..32029
                     /gene="frlC"
                     /locus_tag="OO244_000349"
     CDS             31199..32029
                     /gene="frlC"
                     /locus_tag="OO244_000349"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5662299.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32026..32811
                     /gene="frlD"
                     /locus_tag="OO244_000350"
     CDS             32026..32811
                     /gene="frlD"
                     /locus_tag="OO244_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5662300.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32911..33642
                     /gene="phoB"
                     /locus_tag="OO244_000351"
     CDS             32911..33642
                     /gene="phoB"
                     /locus_tag="OO244_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5662301.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33802..34887)
                     /locus_tag="OO244_000352"
     CDS             complement(33802..34887)
                     /locus_tag="OO244_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5662302.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34899..36203)
                     /locus_tag="OO244_000353"
     CDS             complement(34899..36203)
                     /locus_tag="OO244_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5662303.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36215..36568)
                     /locus_tag="OO244_000354"
     CDS             complement(36215..36568)
                     /locus_tag="OO244_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5662304.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36579..37457)
                     /locus_tag="OO244_000355"
     CDS             complement(36579..37457)
                     /locus_tag="OO244_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5662305.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37454..38680)
                     /locus_tag="OO244_000356"
     CDS             complement(37454..38680)
                     /locus_tag="OO244_000356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5662306.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVECRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38681..39844)
                     /locus_tag="OO244_000357"
     CDS             complement(38681..39844)
                     /locus_tag="OO244_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5662307.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39928..40290)
                     /locus_tag="OO244_000358"
     CDS             complement(39928..40290)
                     /locus_tag="OO244_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5662308.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40307..41212)
                     /locus_tag="OO244_000359"
     CDS             complement(40307..41212)
                     /locus_tag="OO244_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662309.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41482..42486)
                     /gene="trpS"
                     /locus_tag="OO244_000360"
     CDS             complement(41482..42486)
                     /gene="trpS"
                     /locus_tag="OO244_000360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5662310.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42479..43237)
                     /gene="gph"
                     /locus_tag="OO244_000361"
     CDS             complement(42479..43237)
                     /gene="gph"
                     /locus_tag="OO244_000361"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5662311.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43230..43907)
                     /gene="rpe"
                     /locus_tag="OO244_000362"
     CDS             complement(43230..43907)
                     /gene="rpe"
                     /locus_tag="OO244_000362"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5662312.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43925..44761)
                     /gene="dam"
                     /locus_tag="OO244_000363"
     CDS             complement(43925..44761)
                     /gene="dam"
                     /locus_tag="OO244_000363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5662313.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44868..46160)
                     /gene="damX"
                     /locus_tag="OO244_000364"
     CDS             complement(44868..46160)
                     /gene="damX"
                     /locus_tag="OO244_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5662314.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46252..47340)
                     /gene="aroB"
                     /locus_tag="OO244_000365"
     CDS             complement(46252..47340)
                     /gene="aroB"
                     /locus_tag="OO244_000365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5662315.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47397..47918)
                     /gene="aroK"
                     /locus_tag="OO244_000366"
     CDS             complement(47397..47918)
                     /gene="aroK"
                     /locus_tag="OO244_000366"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5662316.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48319..49557)
                     /gene="hofQ"
                     /locus_tag="OO244_000367"
     CDS             complement(48319..49557)
                     /gene="hofQ"
                     /locus_tag="OO244_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5662317.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49469..49875)
                     /gene="hofP"
                     /locus_tag="OO244_000368"
                     /pseudo
     CDS             complement(49469..49875)
                     /gene="hofP"
                     /locus_tag="OO244_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49865..50305)
                     /gene="hofO"
                     /locus_tag="OO244_000369"
     CDS             complement(49865..50305)
                     /gene="hofO"
                     /locus_tag="OO244_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5662318.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50289..50828)
                     /gene="hofN"
                     /locus_tag="OO244_000370"
     CDS             complement(50289..50828)
                     /gene="hofN"
                     /locus_tag="OO244_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5662319.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50828..51607)
                     /gene="hofM"
                     /locus_tag="OO244_000371"
     CDS             complement(50828..51607)
                     /gene="hofM"
                     /locus_tag="OO244_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5662320.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51727..54279
                     /gene="mrcA"
                     /locus_tag="OO244_000372"
     CDS             51727..54279
                     /gene="mrcA"
                     /locus_tag="OO244_000372"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5662321.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54444..55004)
                     /gene="nudE"
                     /locus_tag="OO244_000373"
     CDS             complement(54444..55004)
                     /gene="nudE"
                     /locus_tag="OO244_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5662322.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55324..57459
                     /gene="igaA"
                     /locus_tag="OO244_000374"
     CDS             55324..57459
                     /gene="igaA"
                     /locus_tag="OO244_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5662323.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57524..58192
                     /gene="yrfG"
                     /locus_tag="OO244_000375"
     CDS             57524..58192
                     /gene="yrfG"
                     /locus_tag="OO244_000375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5662324.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58203..58604
                     /gene="hslR"
                     /locus_tag="OO244_000376"
     CDS             58203..58604
                     /gene="hslR"
                     /locus_tag="OO244_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5662325.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58629..59498
                     /gene="hslO"
                     /locus_tag="OO244_000377"
     CDS             58629..59498
                     /gene="hslO"
                     /locus_tag="OO244_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5662326.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59642..61366)
                     /locus_tag="OO244_000378"
     CDS             complement(59642..61366)
                     /locus_tag="OO244_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5662327.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61745..63367
                     /gene="pckA"
                     /locus_tag="OO244_000379"
     CDS             61745..63367
                     /gene="pckA"
                     /locus_tag="OO244_000379"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5662328.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63443..64795)
                     /gene="envZ"
                     /locus_tag="OO244_000380"
     CDS             complement(63443..64795)
                     /gene="envZ"
                     /locus_tag="OO244_000380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5662329.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64792..65511)
                     /gene="ompR"
                     /locus_tag="OO244_000381"
     CDS             complement(64792..65511)
                     /gene="ompR"
                     /locus_tag="OO244_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5662330.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65739..66215
                     /gene="greB"
                     /locus_tag="OO244_000382"
     CDS             65739..66215
                     /gene="greB"
                     /locus_tag="OO244_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5662331.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66312..68633
                     /locus_tag="OO244_000383"
     CDS             66312..68633
                     /locus_tag="OO244_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5662332.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69071..69298
                     /gene="feoA"
                     /locus_tag="OO244_000384"
     CDS             69071..69298
                     /gene="feoA"
                     /locus_tag="OO244_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5662333.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69315..71636
                     /gene="feoB"
                     /locus_tag="OO244_000385"
     CDS             69315..71636
                     /gene="feoB"
                     /locus_tag="OO244_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5662334.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71636..71872
                     /gene="feoC"
                     /locus_tag="OO244_000386"
     CDS             71636..71872
                     /gene="feoC"
                     /locus_tag="OO244_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5662335.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72075..>72932
                     /gene="rpnA"
                     /locus_tag="OO244_000387"
     CDS             72075..>72932
                     /gene="rpnA"
                     /locus_tag="OO244_000387"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5662336.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEE"
BASE COUNT        17573 a        19759 c        18453 g        17147 t
ORIGIN      
        1 ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga gaagtgttga
       61 tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat gcacgagcag
      121 caccgccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta gttttaccgt
      181 ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca aatttttctt
      241 tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc acgggacttt
      301 ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc aacgttactc
      361 ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt ggtcagagaa
      421 cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg gatcttaacg
      481 ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa gtcaccgata
      541 acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg ctcaagcaga
      601 actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag tttaaacgcc
      661 agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc catgtctact
      721 accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt atcaacggcc
      781 gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc gtagcctttc
      841 gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata ctgaccacga
      901 ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg gatagtttca
      961 cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg cttcatacgg
     1021 tcaacgatga tgtcgaggtg cagttcgccc atacccgcga tgatggtctg gttagattct
     1081 tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag agccagaccc
     1141 attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac cggctcaggg
     1201 aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc accagtggtt
     1261 acgtctttca ggccgatagc agcagcgatg tcgcccgcgc gaacttcttt gatctcttca
     1321 cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc tttcacggag
     1381 ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa ggtcaggtta
     1441 ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc gtcatcactt
     1501 gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat cgcaggtacg
     1561 tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac acctttgttc
     1621 ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg ctgacgcaga
     1681 gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc catcagctct
     1741 tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc cagttcaacc
     1801 atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc gttccagttg
     1861 atagctttca ttttcaccag gtcaacaaca ccggtgaaat gttcttcagc accaatcgcc
     1921 agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac aactttcagg
     1981 aagttcgcac ccacgcggtc cattttgtta acgaacgcaa tgcgcggaac tttatatttg
     2041 tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc gcagtaaacc
     2101 attaccgcac catcgagaac acgcatggaa cgttctactt cgattgtgaa gtcaacgtgc
     2161 cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat accagaccag
     2221 aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg ctccatccag
     2281 tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc ggtgtagaac
     2341 agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat accgatgtta
     2401 cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct tttaggcgtt
     2461 caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg actaaaactc
     2521 cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat acggtgaacg
     2581 tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag ttcgttcgcc
     2641 aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat ccaacgcatt
     2701 gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt agaaccacca
     2761 acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac ttcgaatgct
     2821 tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta tacgatagat
     2881 tcagcagtag attttttacc atctaccatc aggatattta caaatttagc cagcagttct
     2941 gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg acgtggcatg
     3001 gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt ttgacattta
     3061 agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg cttcacgcca
     3121 tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc accacgtacg
     3181 gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag gatcacggag
     3241 tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa accgttagtc
     3301 agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt ggtagtatat
     3361 acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac gttgcttttc
     3421 gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat taaatagctc
     3481 ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa tatgaggacg
     3541 ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc cgccatcacc
     3601 aggccatctg gctggagtgc ttaaccgtaa gtctgacgaa atcagtatag tcaatgggaa
     3661 tgatgtcgtt caaaatttga ccagtcaaac cgcgggcaat aaggtcttcg ttcagggcat
     3721 agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca actgcggcag
     3781 ttacgccatc ttgcaataat agcagttcgt ctccttcact gagcagacgc agcagcgcag
     3841 caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc tcaaaacctc
     3901 aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag cggcgtggct
     3961 tcgacaacaa agggtgtctg cggatctaac ccgcgttcgc gcagtgaagc cgcacaaacc
     4021 cagcactgtt caatgtcgta cagactcaac aatttaaaag tggcaatgta atcacgcgcc
     4081 agcactgcat cgggcttttg tcctgacagc agctgaaaaa cgccatcagc aataaagaag
     4141 acggccagat cgtcagttaa tgcggaagtt gccagtaaag catctaaacc ttcccgacca
     4201 gcagctgtac catgaggtgc agtagaaaaa acaaacgcaa ttcgtttcat cagaactgta
     4261 ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt aaggtaaatc
     4321 ccggctgaag gtttgacgaa gccagcccca gccttcctgc ttcagtttca tcgacgatac
     4381 cgcggcgtaa cgctgccgct acgcagatat tcagcgccac accatgttgc gcattcaact
     4441 gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcagt tggttagcgt
     4501 tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct gctatcagcg
     4561 cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc ccggtcacca
     4621 cgatggcaaa acgcattact tatcttgccc ctgaaaatca ccgctcttga actggcggat
     4681 gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt tgatcgcatc
     4741 tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat ttttggcgtt
     4801 attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg tttgggtaac
     4861 cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg tttctggcgg
     4921 cacaaaagtg ctcataatct gcgagaaggg aacatcaaga ttcatattga tgcacagcag
     4981 accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca ttaatacgcc
     5041 gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg tcatatcgtg
     5101 cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg tatgttcacc
     5161 gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa cgatttcaca
     5221 gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt tcagaatatc
     5281 aaaatcggtc tggtcgaaag gacgttgatc cagtaaatcc aactcactgg tttcgttggt
     5341 taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg ttaacttttc
     5401 agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt ccgctttcca
     5461 gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg gcggttctta
     5521 atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt gcatcagcct
     5581 tcggcgctgg tttcacatcc agcagctcta cgtcaaacac tagggtagaa ttcggtggga
     5641 tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt ttgatcttac
     5701 cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg tccagacgga
     5761 aagaaagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg atcagcgtac
     5821 ctttgtagtt cactacaaca gtatcgctgt ctttcggtgc ttcgccttta ccggcttcta
     5881 ctacctgata aaccagacca gttgaagagg ttttcacacc tttctctttg gcaaatttct
     5941 cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc ttcgcctgag
     6001 cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct tggtcggaga
     6061 gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct ttatccagtt
     6121 tgatgcccag tttttcttgt tctttaagag agttttccat gtaacgaccc agcgaagcac
     6181 ccagtgcata agctgatttc tgatcgtcat ttttgaacga tgctttgctg tctgcagttg
     6241 tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcagggca acggccattg
     6301 tggtcgccag tagcgttact ttaaacagtg atttcatcca tatctccagg atcggggcat
     6361 ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga acaaatctac
     6421 atacagacac gccctattat catctatttt cagactcttt ttgtttaaat tagtttcgat
     6481 gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg gcgaccattc
     6541 gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca gagctggaga
     6601 gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg accgctcatg
     6661 aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag ttaaaagcca
     6721 gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat tattgaggcg
     6781 taaaaaaagc ggggattccc cgcttttttg tcaccttttc ggtattagtg gcaaccacaa
     6841 ccgccgttgc ctttaccgcc acagcagcct tcgccaccgt gttcatgacc gtgatcgtgg
     6901 ccatgaccgc cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg cgcgccgtga
     6961 acgtggccat gagccagttc ttcttcggtc gcttcgcgaa tcgccacaac ttcaacgttg
     7021 aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccactacgtg atcgtcttca
     7081 accgcagtga tttcaaccgg taccggaccc tggtcagttt cagccaggaa acgcatacct
     7141 acctgcagtt catcaacgcc cataaatacg tctttaggaa cacgttgcac taggttttcg
     7201 tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc gccaacttca
     7261 tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg caggtagtcc
     7321 agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg tacctgatag
     7381 gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca tgggaagaat
     7441 agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa aaaaactcgg
     7501 cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgt gaacgtgatc
     7561 gcgggcttct ttgtctgctt ctcgcatctg atgtccgcac ttaacacatt caacaatatc
     7621 aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacaggccg ggcattttgc
     7681 gcccgctata aaacgttttc ggattgccat ccttaccctc tactcaaatt catcccagcc
     7741 gtccagctgg cgtcgttctt gttgcatttc acgctggaaa atctcttcca gttcgcgtcg
     7801 ggcttccctg gcgcgagaaa tttgtacggt atcagcatgc atcgggatta actcccgcag
     7861 cattcgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat gcggatgcat
     7921 gccaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg tttcacggga
     7981 aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc gcgctcgcgc
     8041 aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca tggtgtcttc
     8101 cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag aacgtaaaag
     8161 atcgacctgc gtggcgtcgc cgtaataaac tttgtagcca tatttacgca tcaggttaac
     8221 ggcgctgata tcccgctcca gcacggtaat gcgcatttta tttgccatca gcaaacgacc
     8281 aatcacctga ccaaaacgcc cgaagcccac gacaatgacc tggggtttat catcgttgac
     8341 ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc atttatccac
     8401 cagcttcatc agcaacggcg ccatctggtc gccctggaat aaacgttgtg aagaagcggt
     8461 agaaaagagg acaaaggcaa actcaccgcc ctgactcaac acgccagcaa actgcatccg
     8521 ctctgagcta cgcacgccat acaatcgcgc cagcagatac agcacgagaa ttttcaccgc
     8581 caccagcaca accacgctta tcactaccca caacagatgg atataaagca ccccgaggtt
     8641 gagcgacatg ccgacagaga taaagaacaa accgagcagc aagcctttga agggatcgat
     8701 agccgtttcc agttcatggc gatattcact ttccgccagc agcacgcccg caataaatgt
     8761 accgagcgcc atcgacagcc ccagcgcatc cataaacaat gcggaaccca acaccagcag
     8821 cagcgtcgcg gcggtgaaca cttcccgcac gccagaagct gcaataaagc ggaataccgg
     8881 acgcagtaaa tagcgcccac caatcagcat gccgacaaac tccagcacct tcatgccgat
     8941 cttcatccag tcgaaatgtt cgtctgccga ccccgccaac aacggcacta acgccagtgc
     9001 tgggatcact gccagatcct gaaacagcag aaccgaaaac ccgagctggc cggattcgct
     9061 gcgattcatc cctttctcac gcatcaattg caacgccatt gcagttgaag acatcgcaaa
     9121 gccaatgcca ccgaccaccg ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc
     9181 cagcaacgcc gcgcttaaca gcacctgtgc cgcgcctacg ccaaaaatcg aacgccgcag
     9241 ttgccaaagt ttggagggat tcaactcaag gccgatgata aacatcagga ataccacgcc
     9301 gagttccgaa aagtggagga tctcatcgac gtcgctaata aaccccagcc cccacgggcc
     9361 aattgcaatc cccgccagca aatatcccaa cacagcgcca atccccagcc gcgaagagaa
     9421 acagcactcc tgcgagtaaa aaatcggaac cttccatcag cggcctcctg gagacagcgg
     9481 atttgccaac cagtcaccgt aggctctggc gtggctcgcc agctcctttg cgctttgccg
     9541 tctcgcccag taaataatga tgggacttaa ccaatgcatc cggcacatgc ccgccgccag
     9601 ttcaaagggg cgcagcacat cgctcatcgg gtagcgattc agcgcgtcat aacggtaagc
     9661 actttccggc tcgccggtgg taatcacgtt acgccagtac tttcccgcca gttggtttcc
     9721 tcccggcccg ctggcaaaac cacgacttaa tacccggtcc agccactctt tcagtagcgc
     9781 cgggcagcta taggtataaa gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa
     9841 tgcctgctca cgggggatat caataaaaaa atcgggatag tgcgcgtaaa ggtcgtgcac
     9901 ggtaacattg ctgagctgcg tggccggttt aagcagtacc cggtttgcca ccgagtcctg
     9961 agattccgga tgggcataca gcagcaaaac tttcgctggc tgagacatca tccccctccc
    10021 ggtatggttt ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt gcggcagctc
    10081 gcccatacat tacattatca taatgataag ttaacatagt ctgaacatac ggcgccttat
    10141 gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac
    10201 cgccaccatc aaccccgggc agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc
    10261 taccctgctg gcattgctga aaaatgaaat cagcgccgac ggcggcagct acacctttcc
    10321 gggaagctgg caactggcgt gggtgaatca ggaaacgcca gcgttaccgc aagcggcgct
    10381 ggaatatgtc attgacggcg accgtgaata tcgtcaactg gaagcgcagc tgcacgacgc
    10441 caacgaacgt aacgacgggc acgccattgc gaccattcat agcaagctgg atgctattga
    10501 cgcatggagt attcgctccc gtgccgccag cctgctgcac ggcctcggtt tcagcaatga
    10561 acaactggag cgcccggtaa gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc
    10621 ccaggcgctg atttgccgtt cagacttgct gctgctcgac gaaccgacta accacctcga
    10681 tctcgatgcc gttatctggc tggaaaaatg gttgaagagc tatcagggca cgctgatcct
    10741 gatctctcac gaccgcgact tcctcgatcc gatcgttgat aaaattattc atatcgaaca
    10801 acaaagcatg ttcgagtaca ccggcaacta cagttcgttt gaagtacagc gcgccacccg
    10861 tctggcgcag caacaagcga tgtacgaaag ccagcaggaa cgcgtggcac atctgcaaag
    10921 ttatatcgat cgtttccgtg ccaaagccac caaagcgaag caggcccaga gccgcattaa
    10981 gatgctggaa cgtatggagc tgattgcccc cgcacacgtc gacaacccgt tccgctttag
    11041 cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag atggaaaagg tcagcgcagg
    11101 ctatggcgat cgcattattc tcgactcgat taaattgaat ctggtccccg gctcgcgcat
    11161 tggtctgtta ggccgcaacg gcgcaggtaa atcgacatta atcaaactgt tagccggtga
    11221 acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg atcaagctcg gctacttcgc
    11281 ccagcatcaa cttgaatacc tgcgcgccga cgaatcacct attcaacatc tggcacgttt
    11341 agcgccgcag gagctggaac aaaaactgcg tgactacctc ggcggctttg gtttcctggg
    11401 cgataaagta accgaagaaa cgcgccgctt ctccggtggg gaaaaagccc gcctggtgct
    11461 ggcattaatt gtctggcagc gtccgaatct gctgctgctc gacgaaccga ctaaccacct
    11521 tgacctcgac atgcgtcagg cactcaccga agcattaatc gagtttgaag gcgcgctggt
    11581 tgtcgtttcg cacgaccgtc atttgctgcg ttccaccact gacgatctct acctggttca
    11641 cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat tatcaacagt ggttgagcga
    11701 cgtacaaaag caggaaaacc agaccgacga agcgccaaaa gagaacgcga acagcgccca
    11761 ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc agccgctgcg
    11821 taaagagatt gcccgtctgg aaaaagagat ggagaagctg aacgcgcaac tggcgcaggc
    11881 ggaagagaaa ctcggcgaca gcgaactgta tgaccagagc cgtaaagcgg agttgaccgc
    11941 ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct
    12001 ggaagcccag gagcagcttg agcagatgct gctggaaggc caaagcaact gatggcgcag
    12061 ataacgacga ccgatgccaa tgaatttagc agcagtgctg aattcacccc tatgcgcggc
    12121 tttagcaatt gtcatctaca aactatgctg ccgcgtttgt ttcgtcgtca ggtgaaattc
    12181 accccgtact ggcagcggct ggagttgccc gacggcgatt ttgtcgatct cgcgtggagt
    12241 gaagaccccg cacaggcgaa acataaaccg cgtttagtgg tgtttcacgg gctggaaggc
    12301 agtctcaata gcccttacgc ccacggtctg gttgaggcgg cgcaaaagcg cggctggctg
    12361 ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa accgtatgca ccgcatttac
    12421 cattcgggcg aaaccgaaga cgccagttgg tttttacgct ggctgcaacg cgaatttggt
    12481 catgcgccaa cggctgccgt cggctattcg ctcggcggta atatgctggc ctgtttgctg
    12541 gcaaaggaag gcaatgatct cccggttgat gcggcggtga ttgtctctgc gccgtttatg
    12601 ctggaagcct gtagctatca tatggaaaag ggcttttccc gcgtttatca gcgttacttg
    12661 ctgaacctgt taaaagccaa tgccgcgcgc aagctggcag cctaccccgg aacgctgccg
    12721 attaatctcg cgcagttaaa atcggtacgt cgcatccgtg aatttgacga tctaatcacc
    12781 gccagaattc acggctacgc cgacgctatc gactattatc gtcagtgtag cgccatgcca
    12841 atgctgaacc ggatcgccaa accgacgctg attattcacg ccaaagacga tccgtttatg
    12901 gatcatcagg tgatcccaaa accggaaagt ctcccctcgc aggtggagta tcaactgact
    12961 gaacatggcg gtcatgttgg ctttattggc ggtacattac ttcatccgca aatgtggctg
    13021 gagtcacgca ttcctgactg gttaacaacg tatctggagg cgaaatcatg ttgattccgt
    13081 ggcaagacct ctcccccgaa acgctggaaa atttaattga aagctttgtg ttacgtgaag
    13141 gcaccgatta tggtgaacat gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc
    13201 agctacagtg cggagaagcg gtgctggtat ggtcggagct gcacgaaacg gtcaatatta
    13261 tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg cggacgagat aatagtgata
    13321 gctaagtcat ggagttacta tgtctgccaa acatccggtc attgcggtaa caggatccag
    13381 cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct
    13441 gcatgcagct gaggtggaag gcgacagttt tcaccgttac acccgcccgg aaatggacat
    13501 ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa
    13561 cgacttcggc ctgctggaac aaaccttcat tgaatacggt cagagcggca aagggaaatc
    13621 tcgcaaatat ctgcatacct acgacgaagc cgtaccctgg aatcaggtac cgggaacatt
    13681 caccccctgg caacctttac cagaacccac tgatgtactg ttttatgaag gtttacacgg
    13741 cggcgtagtc acgccacagc ataacgttgc gcagcatgtg gacttactgg tcggcgtagt
    13801 gcctatcgtt aaccttgagt ggattcaaaa actgatccgc gacaccagcg agcgcgggca
    13861 ctcacgagaa gcagtgatgg actcggtagt gcgttcaatg gaagactata tcaactacat
    13921 cacaccgcag ttttcccgca cccatcttaa cttccagcgc gttcccaccg tcgacacttc
    13981 aaacccgttc gcggcaaaag gtatcccgtc gctggatgaa agctttgtgg tgatccattt
    14041 tcgtaatctg gaagggatcg atttcccttg gctgctggcg atgttgcaag gctcattcat
    14101 ttcccacatc aatacgttag tggtacctgg cggcaaaatg gggctggcaa tggaattaat
    14161 tatgctgccg ctggtgcaac gattgatgga aggaaagaaa atcgagtaac tttgctatta
    14221 tgccggataa aatactatcc ggcttcacaa cgggatagtt aagtcacgcg gcaaccactt
    14281 catacgagtg agtaatattc accgcttttt ccagcatcaa cgccactgaa caatatttct
    14341 cggcagagag atcaaccgca cgcgcaaccg ctgcgtcttt caggtcgcga ccggtgacga
    14401 taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgt tcagaggtca
    14461 attttacttc acaatcgacc acatcctgac gccctttttg caggatcgaa accacatcga
    14521 tggcactgca accacccgcc gccatcaaca ccatttccat cggacttggt gctttatcgc
    14581 ctgagttgcc gtccattaaa atctggtgac cagaggcgga ttcgcccaga aaagttaacc
    14641 cttcgaccca cttcactcgc gcttgcattt ttgctactcc actgcgtcaa ttttcctgac
    14701 agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg ctgaagcgag
    14761 acaccaggag acacaaagcg aaagctatgc taaaacagtc aggatgctac agtaatacat
    14821 tgatgtactg catgtatgca aaggacgtca cattaccgtg cagtacagtt gatagcccct
    14881 tcccaggtag cgggaagcat atttcggcaa tccagagaca gcggcgttat ctggctctgg
    14941 agaaagctta taacagagga taaccgcgca tggtgcttgg caaaccgcaa acagacccga
    15001 ctctcgaatg gttcttgtct cattgccaca ttcataagta cccatccaag agcacgctta
    15061 ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt taaaggctct gtggcagtgc
    15121 tgatcaaaga cgaagagggt aaagaaatga tcctctccta tctgaatcag ggtgatttta
    15181 ttggcgaact gggcctgttt gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa
    15241 ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg ccaattgatt caggtaaacc
    15301 cggacattct gatgcgtctg tctgcacaga tggcgcgtcg tctgcaagtc acttcagaga
    15361 aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat tgcacagact ctgctgaacc
    15421 tggcaaaaca accagatgct atgactcacc cggacggtat gcaaatcaaa attacccgtc
    15481 aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc
    15541 tggaagatca gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt
    15601 aatcccgtcg gagtggcgcg ttacctggta gcgcgccatt ttgtttcccc cgatgtggcg
    15661 cagactgatt tatcaccccg atatcaacta tgcacttcga caaacgctgg tgctatgttt
    15721 gcccgtggcc gttgggttaa tgcttggcga attacgattc ggtctgctct tctccctcgt
    15781 tcctgcctgt tgcaatattg cgggccttga tacgcctcat aaacgttttt tcaaacactt
    15841 aatcattggt gcgtcgctgt ttgccacctg tagcttactg acacagctgc tactggtaaa
    15901 agatgttccc ctgccctttt tgctgaccgg attaacgctg gtacttggcg tcactgctga
    15961 gctggggcca ttacacgcaa aattgcttcc tgcatcgctg ctcgccgcca tttttaccct
    16021 cagtctggcg ggatacatgc cggtctggga accattgctc atctatgcgt tgggcactct
    16081 ctggtacgga ttgtttaact ggttttggtt ctggatctgg cgcgaacaac cgctgcgcga
    16141 gtcactaagt ctgctgtacc gtgaactggc agattattgt gaagccaaat acagcctgct
    16201 tacccagcac accgatcctg aaaaagcgct gccaccgctg ctggtgcgcc agcaaaaagc
    16261 ggtcgattta attactcagt gctatcagca aatgcatatg ctttccgcgc aaaacaatac
    16321 cgattacaag cggatgctgc gtattttcca ggaggcgctg gatttgcagg aacatatttc
    16381 ggtcagtttg catcagccgg aagaggtgca aaaactggtc gagcgtagcc atgcggaaga
    16441 agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat
    16501 tctttaccat cgcctgccaa cgcgttttac gatggaaaag caaattggcg cactggaaaa
    16561 aatcgcccgc cagcatccgg ataatccggt tgggcaattc tgctactggc atttcagccg
    16621 catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc cgtgacttac tggccgataa
    16681 acagcggcga atgccgttgc ttccggcact gaaaagttat ctgtcactaa agtctccggc
    16741 gctacgcaat gccggacgac tcagtgtgat gttaagcgtt gccagcctga tgggcaccgt
    16801 gctgcatctg ccgaagtcgt actggatcct gatgacggta ttgctggtga cacaaaatgg
    16861 ctatggcgca acccgtctga ggattgtgaa tcgctccgtg ggaaccgtgg tcgggttaat
    16921 cattgcgggc gtagcgctgc actttaaaat tcccgaaagt tacaccctga cgttgatgct
    16981 gattaccacc ctcgccagct acctgatatt gcgcaaaaac tacggctggg caacggtcgg
    17041 ttttactatt accgcagtgt ataccctgca actgttgtgg ctgaacggtg agcagtacat
    17101 ccttccacgt cttatcgata ccattattgg ttgtttgatt gctttcggtg gtaccgtctg
    17161 gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac gcccatgatg ctttagaagc
    17221 ctatcaggaa gcgattcgct tgattctgag cgaggacccg caaccaacgc cactggcctg
    17281 gcagagaatg cgggtaaatc aggcgcataa cactctgtat aactcattga atcaggcgat
    17341 gcaggaaccg gcattcaaca gccattatct ggcagatatg aaactgtggg taacgcacag
    17401 ccagtttatt gttgagcata ttaatgccat gaccacgctg gcgcgggaac accgggcatt
    17461 gccacctgaa ctggcacaag agtatttaca gtcttgtgaa atcgccattc agcgttgtca
    17521 gcagcgactg gagtatgacg aaccgggtag ttctggcgat gccaatatca tggatgcgcc
    17581 ggagatgcag ccgcacgaag gcgcggcagg tacgctggag cagcatttac agcgggttat
    17641 tggccatctg aacaccatgc acaccatttc gtcgatggca tggcgtcagc gaccgcatca
    17701 cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg taatacaggc taaaccatcg
    17761 gataaggcat tcacgccgca tccgacatat tttgcctgat acgtcttatt tacgccccaa
    17821 ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccttc atcgatatcc gcatcttcca
    17881 ccaccagcga cggtgctaaa cgcatcacat ccggtccggc attcagcacc attacgccaa
    17941 cctctgcacc cgcatacagg aaatcacgcg cccgaccttt gtagtgtggt ttcagctccg
    18001 cgccaattaa cagccccata ccgcgaatat cgctaaacac atcgtactgc tgatcgatct
    18061 tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc acttcagggg
    18121 tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg
    18181 tggaaccgtg agaaccagga tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc
    18241 taatcgggaa gccgccgcct aacgctttcg cagaagtcag aatatccggc gtcacaccgt
    18301 agtacctgta agcaaacaaa tcgccagtcc gccccatccc gcactgcact tcatcaaaca
    18361 ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg
    18421 ctgccgtcac accgccctcg ccctggatcg gctcaaccac caccgcacag gtgtgatcat
    18481 ccatcaccgc tttcactgca tggagatcgt taaagggaac gtggatgatg tctgccggtt
    18541 tcggcccaaa gccgtcggaa tattttggct gcccacccac cgaaacggta aacagcgagc
    18601 gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacgggcta tgacgcacac
    18661 aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa
    18721 acaccacgcg ttcggcaaac gtcgcctcaa tcagtttgcg cccaagacgc agcgccggtt
    18781 cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt aacgcgttca
    18841 ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg aaatcgacat
    18901 actccttgcc ttgctgatcc cagattcggc tgccctgacc ttttaccgga ataaactctg
    18961 ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt
    19021 caattgccat ctcatgatca ccctgttacg cataaataaa tgtgaaatta taatcacaaa
    19081 atatgcataa aaaatcactg aatggcaatc ggaaatcaga gatgcaggaa attagccagc
    19141 agttgatgtc cttgttcgct aagaatactt tctggatgga actgcacacc ttccagatcc
    19201 cactggcgat ggcgaatccc cataatctcg cgggtttcgc tccaggccgt cacgtcaaag
    19261 cacgctggta atgagtcagg ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga
    19321 tttgccagcc cccgaaatac gccctcaccg ctatgtgtaa tcggcgaggt tttgccgtgc
    19381 atgacctttg cggcgcgcac aactttgccg ccaaatgcct gcgccattgc ctgatgaccg
    19441 aggcagacgc caagaatcgg caagcgcccg gcatagtggc gaataacgtc aagggagatc
    19501 ccggcttcat ctggcgtaca ggggccaggt gagatgacaa ttttttgtgg tttaagggcg
    19561 tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc ccccagttca
    19621 caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag caggatcatg
    19681 gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccggctt cgcttaccac
    19741 tttgctaaat atcatctgca gtgcggtgag atcccccatt gccccactct gatttgcctg
    19801 attccaggcc tctttttcga taccctgcca gctcagttga tatccggcat gaatcgccag
    19861 ttgctcgaag aagatccgct gtgccagacc acttcccacc cggaagggat gcagcacgtt
    19921 gatttcacaa tagtaatgcg ccagccgctc gacgaacttc gctttctcca ggccaaccag
    19981 ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga tataagcaaa
    20041 gtggcagaac ggcgtatcgc cctgataaat atcaacttca cgcagttgcc ctgcccagtc
    20101 gaaaatatcc tggtacagct ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag
    20161 cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag
    20221 ccgctgctgc tggcggatgt tcagccggtt acgcatgata tcaaggccag gataaagata
    20281 cagatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct tcaaggcgcg
    20341 ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca agacggcgac
    20401 tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc ttatcggtga
    20461 gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa gcggcaaatc
    20521 cgggttacgc cgggagagca gaagcgcggg caagattgcc cgcgcgagaa atcattacgg
    20581 caggacttta gcggaaagga taactaccgg ttttgacggc acattctggt acggaccaac
    20641 atcatgagtc ggcacctggg aaatcttatc ggcaacgtcc atgcctttca ccactttacc
    20701 aaataccgcg taaccgaaat cacgctgacc atggtcaagg aaggcgttat cggcaacgtt
    20761 gataaagaac tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc
    20821 acgcgtgttg cgcaggccgt tatcggcttc atttttgatt ggcgggtttg gttttttctg
    20881 ctgcatctgc tcggtgaaac cgccgccctg aatcataaag ccaggaatga cgcggtgaaa
    20941 ggtagtgttg ttataaaaac cgctgttcac ataatcgaca aagttttgca cagacactgg
    21001 cgctttttgt ttatccagct ccagttcgat gttaccagct gaggttgtca acaatacgtg
    21061 cgggtcccct ttcgctgcca ttgctgcggg agaaagagca gaaagagcag aaagagcgaa
    21121 aacagcagcc atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca
    21181 gaaaaaaagc aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct
    21241 aattttacgt atctgaaata tatgtaacct gacgatcact ttttattccg tgatcaaaat
    21301 cacctcttaa aatgcaattt agcaaccgat tgcaataaaa catttaaaca gatcataaaa
    21361 tcacctaaaa tcgcccgctc gcggcatatc ccgctatgcc attttatttt tgaacgcagg
    21421 ccaatcatga ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ctcctacgca
    21481 ctgactggtg cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc
    21541 aatctgcctg tttccagtat gagtaacacc ttcaccttcc tcaacgccgg cattttaatc
    21601 tctatcttcc tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt
    21661 ggctttctcc tgatggtgct ggcggttgtc ggtttgatgt tcagccacag cctggcgctg
    21721 ttctcgacgg cgatgttcat tctcggggtg gtcagcggca ttactatgtc gattggtaca
    21781 ttcctgataa cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt atttactgac
    21841 tccttcttca gtatggctgg gatgattttc ccaatgatcg ccgcgtttct actggcgcgc
    21901 agcattgagt ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt
    21961 ctgaccttcg gctgtgagtt cccggcactg ggcaaacgtg caccaaaaac ggatgctccg
    22021 gtagagaaag aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac
    22081 atcctcggtc agttaggttt tatctcctgg gtgcctgagt atgccaaagg cctgggcatg
    22141 agcctgaacg acgcgggcac gctggtgagt aacttctgga tgtcatacat ggtcggcatg
    22201 tgggcgttca gctttattct tcgcttcttt gatttgcaac gcattctgac cgtactggct
    22261 ggtctggctg cgattctgat gtacgtcttt aacaccggaa caccagtaca tatggcgtgg
    22321 tcaattctcg ctctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt
    22381 tcacagcaga ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc
    22441 atcggtacta tgttgacctt tgtggttacc ggcccgattg ttgaacatag cggtccgcag
    22501 gcggcactgc ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta
    22561 ggtttcgtca gccgtcaccg tcagcataac accctgacct ctcattaatt gctaatgccg
    22621 gacggcacta tcgtcgtccg gccttttcct ctcttccccc gctacgtgca tctatttcta
    22681 taaacccgtt cattttgtcc attttttgca caaacatgaa atgtcagaca attccgtgac
    22741 ttaagaaaat ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg
    22801 atttacatca ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc
    22861 gaggcaaaaa tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc
    22921 tttatcgaag atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt
    22981 gaagaaccgc gcatcgctta tgaccgcgta cacctctcat cttacttctc tcaccacacc
    23041 gccgaagagc tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg
    23101 gtcggcgaac gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga
    23161 cgtaccgttt tttatgacaa gctgatcatg gcaaccggtt cctacccgtg gatcccacca
    23221 atcaaaggtt ctgatactca ggactgcttt gtctatcgca ccattgaaga cctcaacgcc
    23281 attgaatcct gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt
    23341 ctggaagccg ctggcgcgct gaaaaactta ggtattgaaa cccacgttat cgaatttgcc
    23401 cctatgctga tggcggaaca gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc
    23461 gaaagtatgg gcgtgcgcgt tcacaccagc aaaaacaccc ttgagattgt gcaggaaggt
    23521 gtcgaagcgc gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc
    23581 gtcttctcta ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt
    23641 gctccgcgtg gggggattgt cattaatgat tcctgccaga cttccgatcc ggatatctac
    23701 gccatcggtg aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac
    23761 aaaatggcgc aggtcgccgt tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct
    23821 gaccttagcg ccaagctgaa actgctgggc gtagacgtag gtggtattgg tgatgcgcac
    23881 ggtcgcacgc ctggcgcacg tagctatgtt tacctcgacg aaagtaaaga gatctacaaa
    23941 cgcctgattg tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc
    24001 agcgactacg gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg
    24061 gattctctga ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa
    24121 ctgccggaca gcgcgcaaat ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct
    24181 gccatcaaca aaggctgcca cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact
    24241 ggctgcggtg gctgtatccc gctggtcact caggtactga acgcggaact ggcgaaacag
    24301 ggcatcgaag ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca ggaactgttc
    24361 catttgatcc gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa
    24421 ggctacggtt gtgaagtttg taaaccaacc gtcggttcgc tgctggcctc ctgctggaac
    24481 gaatacattc tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct
    24541 aacatccaga aagacggcac ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc
    24601 ccggaagggc tgatggcagt gggtcgtatc gcgcgtgaat ttaatctcta caccaaaatc
    24661 accggctccc agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggaaatctgg
    24721 cgtcagctga ttgaagctgg cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg
    24781 gcgaaaacct gcgtgggtag cacctggtgc cgctacggtg ttggcgatag cgtcggcctc
    24841 ggcgtggaac tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt
    24901 gtctccggct gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc
    24961 actgaaaaag gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg
    25021 gatctgctgg cagcggatat cgatcgcgaa acgctgatca aatatctcga ccgcttcatg
    25081 atgttctaca tccgtaccgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc
    25141 gaaggcggca tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgct
    25201 catctggaag aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg
    25261 gtcaatacac cgtccgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac
    25321 ccgaacgtgc agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt
    25381 atcccggtaa ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg
    25441 atgacatcct gcctgaaacc ggcgtctgcg cactgttagg tgacaagcag gtagcgattt
    25501 tccgcccgta tcacagcgac caggtgtttg cgatcagcaa cattgatccg ttcttcgagt
    25561 ccagcgtgct atcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc
    25621 cgctgaaaaa acagcgtttt cgcttaagtg atggcttgtg catggaagac gaacagtttt
    25681 ccgtcaaaca ttacgaagca cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt
    25741 ttaacgggag gcgcaatgcc tccccttttt gcatggtcct gtaatgacct tcggtatatt
    25801 gcaggacatt ttttaaactt tttgttttat tttttgtttt tattttttaa aggataatca
    25861 aatgtttaca gacactatta ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc
    25921 ggcaaataac ccgctcggct tttgggtcag ctccgccatg gcgggcgcat atgtgggtct
    25981 tgggatcatc ctgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt
    26041 gatgggcgcg acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt
    26101 caccggacac accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca
    26161 aatgtgggca atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc
    26221 catgctctat agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc
    26281 cgtcgcgctg gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg
    26341 taactggctg gtttgcctgg caatctggat ggcgctgcgc actgaagggg cggcgaaatt
    26401 tatcgctatc tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc actctatcgc
    26461 taacatgacg ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc
    26521 gggtattggt cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt
    26581 catgggattg ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt
    26641 taatcaaact gaaacagctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc
    26701 tatattttgc caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga
    26761 acgcaaagca aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt
    26821 tattccacag ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt
    26881 tgatgaaagc cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacacgct
    26941 taaccagcgc gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tagttgatgc
    27001 gccgaaagcc gccagcttta ttatgccgtc gattattgac cgctcaccgc taatggtagc
    27061 ggtctcctct ggcggcacct ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc
    27121 actgctgccg ttgcatctgg gccaggtggc gaaatatgcc gggcaattac gcgggcgagt
    27181 gaaacaacag ttcgccacga tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa
    27241 cgaccgcctg gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaacgaccga
    27301 acagttaatc aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc
    27361 gggcgatgcc gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt
    27421 ggtctacgac cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg
    27481 cgttttcgtc ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat
    27541 cctgctgcgg gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt
    27601 tatttttggc cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc
    27661 ggtggttccg ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac
    27721 gcatcgcgat tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga
    27781 gctggactgg gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa
    27841 tcaggccgcg actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt
    27901 ggcaattgtc gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca
    27961 actcggtgaa cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt
    28021 tggcctgcgc gataaactga actggttctc caatcattaa tttaacccgg gccagagaat
    28081 tctggccttc ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa
    28141 atattatctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg
    28201 ctcactgacg ccaattaaat attcctgtat cgttcttaac gagcagtctg cttatattca
    28261 tataatcaat gaatattaat taatatataa tacataggga atgtaaatga acaaatttat
    28321 taaagttgca ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga
    28381 aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa
    28441 aatcattggc ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc
    28501 ggtttaattg cccgacgccc tctggatttt ccgagggcgt atttgtttct atgtccttct
    28561 tgcccccgcc cgtaaaaata ttttcacttt aaattcaatt ttataactac atccattagc
    28621 cacagcgcga tcctgtgcga aattttgtga tcttcctcca cattacataa cataatatgt
    28681 tgttatattc atcatgcatt gtcatgttac cttttaaatg gctgcaaact ctcccctaca
    28741 acgtattgga caagaaaaag gtatcgctat gggaagccag gaactccaac gcaagctcgg
    28801 attttgggcc gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc
    28861 tgtgggtgaa gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat
    28921 tggcgggtta attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc
    28981 ggaaaatggc gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct
    29041 ctccggctgg gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc
    29101 gattgtcagc aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc
    29161 cgccggatta attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc
    29221 gtttcagacg ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg
    29281 gatcttctgg tttaaagcgg agaattttgc cgcccttacc accactgcga ttggcgcaac
    29341 gggcagcttt atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat
    29401 ggcctctatc tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc
    29461 gctgattggt tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc
    29521 cggcctgatg cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac
    29581 ctagatcccc gcactcggca gcaccgctgg gctctttgtt gccatcacgg cgatgatcgt
    29641 cattcttggt tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc
    29701 gaaagacaac ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga
    29761 tgtctccatc atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac
    29821 cagcctgctg ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc
    29881 catcatctgg tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg
    29941 gctgatgacc accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg
    30001 ggcaccgatt cccggcctta tctgcgccgc catcgttatt gctactggtc tgcctgctta
    30061 cgccttctgg gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctgaagaa
    30121 aataaaatgt tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt
    30181 caggaagtgg aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag
    30241 atggtgaagc gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg
    30301 gcgcaaacga cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc
    30361 ggctgggagt tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc
    30421 gtttctgact acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc
    30481 ggagcactca ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa
    30541 tttagcattg attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc
    30601 gtggtgctgg agatgatcac ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac
    30661 gatctcaagc agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc
    30721 cgccagcttg gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg
    30781 ctgcgtccgc tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac
    30841 ggctgcgtga ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc
    30901 gttccgttcc tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt
    30961 aactttgtta aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa
    31021 gggctgcacc cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac
    31081 tacctgtcta tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg
    31141 gaatattaat ccctctcccg gcccggcaac gggccggatt tacgcaagga gttacccgat
    31201 gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga
    31261 tgcaagcgag ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc
    31321 gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat
    31381 gccgattatc ggttacacgc cagaaaccaa cggctatccg tataacatga tgctgggcga
    31441 tgaacatatg cgtcgtgaaa gcctggacat gatcaagctg gcgatagata tggcaaaaga
    31501 gatgaacgcg ggttatacgc tgatttctgc ggcccacgcg ggctatctca cgccgcctaa
    31561 cgttatctgg gggcggctgg cagagaactt aagcgaactg tgtgagtacg cggaaaacat
    31621 cggcatggat ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc
    31681 taatgatgtg cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga
    31741 catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggcga
    31801 taaattacgt catctgcata ttgtcgacag cgacggggcc agcgacacgc attacattcc
    31861 tggagaaggc aaaatgccgc tgcgggaact gatgcgcgat attattgatc ggggctatga
    31921 gggttactct acggtggagc tggtgacgat gtatatgaac gagcccagac tctatgcccg
    31981 ccaggcgctg gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctggcg
    32041 acaatcggcg ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt
    32101 aatgcggtca atgtggcggt gtactgcact cgctacggca tacagccggg atgcattacc
    32161 tgggtgggtg acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc
    32221 gatatcagcc atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac
    32281 gacaatgatc gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt
    32341 gaagaggatt acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat
    32401 gcggaagacg cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc
    32461 gacaagtggg acagcccgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc
    32521 tccgcaccgc aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc
    32581 gcaggaacag tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag
    32641 ttctggcgtc aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg
    32701 ttcattgccg gattcctttg cggctggtct gcggggatga cattaccgca ggcgatggcg
    32761 cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt
    32821 tggcgtgagc atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg
    32881 agtaattcat tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa
    32941 ctcctctacg ctaccgtccg ccagcggctg ctggatgata tcgcgcaggg ggtttaccag
    33001 gccgggcaac agatccctac cgaaaacgag ctttgtacac aatataacgt cagccgcatt
    33061 accattcgca aagccatcag cgacttagtg gcagacggcg tactgatccg ctggcaggga
    33121 aaaggcacct ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt
    33181 accgattttg gcgtctcaca aggcaagtcg acgaaagaga aagtgatcga acaggaacgg
    33241 gtcagcgccg cgccgttttg cgaaaagctg aacatccccg gaaacagcga agtgttccat
    33301 ctctgccggg tgatgtatct cgataaagag ccgctgttta ttgatagttc atggatcccg
    33361 ctgtcgcgtt atcctgactt tgatgagatt tacgtcgaag gaagctccac ctatcagtta
    33421 tttcaggagc gttttgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc
    33481 gccacccgcc cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc
    33541 agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc
    33601 gccaatcgca tcaccttaac gattgataat aaaagacatt aaccgtaggc cggataagat
    33661 gcgccagcat cgcatccggc gatgctggcg cactgaattt tacatcccgc acgttcccct
    33721 caccctaacc ctctccccaa aggggcgagg ggaccgttca gtgcggaatt tagcttgatt
    33781 gccgaacggt gctatccagc attaaatact cgcaatactc tcacgcaaaa tccgcagcac
    33841 cgtctcttca ccgctgcgat tcgggttaat gcgaatcgca caatgttcga gttgtggatt
    33901 cgcctggcga aacgttcctg aaagtcgata gaagagcggc gggatttcat atttcgactc
    33961 tgcaccgacc gggtaaggca acgcgccacg cttttgcgct tcttccagca ccctggcggc
    34021 aatcagctga tgaaactcga caatcaacac cttcgactgc gcattggcaa tgacggcgct
    34081 tttcacttcc gccaccgcgc cgccgttaag caaagccagc aaccgttcgg atactcccgc
    34141 ctgcaccgca tgcatcactg gcgcgaacac cagaccacgc aagacttcca gcgcctgtga
    34201 gccctggatc tggctaccgc cggagtaaag cgtggcgcga atccgactaa taacatcggc
    34261 atcgccaacc actgcaccaa cgccctccgg cccaaacagc ttgaagcagg agaatgtcga
    34321 gacattcgcg ccgcattcac agccgattcg cgctaccttc atcaccgcat agttatcatc
    34381 ggttaacgct ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca gcacatagct
    34441 gtcctgcggc tgctggcgcg tatgctgcac cagcgcggca tcaggttgtt gctcgtcgac
    34501 gacctgcttc agtgccgaca ggtcattgaa atcagcagta ataagcgtca gccccatctg
    34561 ctcaataata acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc
    34621 cggtttgagc aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag
    34681 cgccgcggcc tgtgcgtgaa atgcatcagc cagcacctgc tccacccgtt gggtaatacg
    34741 cggttgattc agacttggag ttaaccctaa atcaccgcca gcaagaaact cgctgcctgg
    34801 aaaatggcgg caaatagtgt ccaccagcgc aaactgcttt tgctgcgctt cagcaagagt
    34861 caggctttgc aaaggaaacg tcttcatagg tgccttcctt aagccggaac gaacaggcca
    34921 agccagtaaa gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc
    34981 attttctgta ccggacggcc cagggattcg ttgaggaagt aaatggcaac cgcgatagag
    35041 aatccggtat aacccgccat cttaattgct gcgaaaatcg aaccgaccag cagcgccact
    35101 tccatcagca tattcatggc gttacggatg ttatccgacg cattacgcac cgacgggtag
    35161 cgtcccagcc atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg
    35221 cctaataccg ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta
    35281 aagcccgcaa ctgcatacac acccgttgct aaggcggtgg tggcaatcaa cggcacaaac
    35341 cccagtccgc gcataaattc tgccagagcc gcctgattaa tcagcgtttg cgattgttcc
    35401 ggcgtcacgc ctgcggaata cgctttctcc agtgtgaaga tcgaaacttc gctaccagca
    35461 aaaatcttca tactggcaac ggcggcaatc aatgctccca cgatggcgat ataaggtaag
    35521 tttttgataa tccgcgacgt gcgctcttca aacaccgaaa gcccgctggc atcgatgtca
    35581 ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca
    35641 ataaagattt cgatggattc agggttaaga tgtgggaaat agcgcacgac taccacacgg
    35701 gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc
    35761 gccaccagcg ggaacagtgc aaaagcagaa accaccggcg agcttaattc cccgaggcta
    35821 cctaatacat ccaccggcag cgcggtcagc agctggttaa ctggcaacag gcaagtaagg
    35881 atcaacacgc cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg
    35941 ccgaggatat cggtaggaag aaagagtaac catgcgttga gcagcccggt tttcagggta
    36001 aaagagatcc ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc
    36061 tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga tcgggtggat gccgtcatga
    36121 aaaacagccg ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg
    36181 ataatctgaa tatacagatc catagtcgtg ccttttattt cagatggtta atcaacatcg
    36241 gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc cactttccct tcagcgatca
    36301 ttttggcgat atgctcgtct ttcgctttaa tgcctggttt ggcaatggtg caacttttgt
    36361 tatagccaat caccgcgatg gcaatcgaca atgccgcgcc cgcaccggta ttacacgcgc
    36421 caatgtaata atctagttgc ccggatttca ccttcattgc cgcttccatg tcgttgtgaa
    36481 tgaaaacttc aaaacagcca ggagccgtcg cttcaatggt ttttttaatc tgctcacgct
    36541 gtaagcctgc aacgccaatc tttttcatga gtctgtcctt attggaaaaa ttgagaggga
    36601 ttttcacgta acatcacatc cacatcggcc tgactgaatc ctgactggcg caattgcgga
    36661 ataaaggtgg tcaataaata gtcatagcca taaccaccat tggcttttaa atgggagcgg
    36721 cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg
    36781 agcatcgcaa tgcgcttttc gtccgggtag taactgttct taccaatggt gtcgaactgc
    36841 acgtacgcgc cgagatcgat catcttcaaa atgttgtcga ggttgtcttt cagatcgcag
    36901 tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt gcaacagagc cagttgttcc
    36961 agccccatgg tgctgaacga cgtatgcgtg gagatcgggc gtccggtctg gttatgcgcc
    37021 agcgcagcgg caataaatac cttctcttcc agcggcgtaa tctttccttc gctggagccg
    37081 atctccgcga tgatcccggc tttcagctcc gtgccatcga taccctgttc aatttcatcg
    37141 accatctcct gcgccagttc ctgcacgctg cgggtcacca catgttccgg gaaaaacgca
    37201 tcctggtaat aaccagtaca ggccaccacg ttgatcccgg tttcgcgcat tacatcgagc
    37261 ataaattgcg cattgcgccc catgtaacgg ttggtcatct caatcacatt acgtacaccc
    37321 cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat actgatcaag gcggcagtcc
    37381 acgttgtttt taaagccgga gagatcaata tgcagatgct catgggccag ggtgtaaccc
    37441 gtcggatcaa aactcatagg gtgtctcctg caaaccggag ggaggaaaga aaagagcgac
    37501 cattttgtgg cggtggcgca cggaaaaatt cacacacggt agctcccaca tcagaaagcg
    37561 tggtgcgcac accgagctgc gtggcgacca gcccttgctg ataaaccagc actggcacca
    37621 cttcacgggt atggtggctg tgaccaatgg tcggatcgtt accgtgatcc gccatcacga
    37681 ccaggcaatc atctggctgc atcgcctcaa caagccgggc aaggttacgg tcaacgacct
    37741 gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga
    37801 tattgatgca aataaacgcc gtcggatggg tgttaaattc gtcgagggtg atatccataa
    37861 tccgctggct atccaccagg ttttgccagc tcacaccata aggattgctg acgatatctg
    37921 ccaccttacc caccagcacg gttggcacgc ccgcttcata cagtttttgc ggcacctgca
    37981 ctttttcatc aacaccgtag cccatatgca cgacctggaa gccgttgtca taagcgccag
    38041 aacgcggcgc attaatacca ataaagcgcc cttctttgct ttctgcggca tcgagaatac
    38101 gctgactgtt ggttaacagg ccgccaaatg taatgacccg accgacctgt acctggtcac
    38161 gcacgatacg accaattttg attgcgtcgt caaaagagat cacagagaga ttggcggtaa
    38221 tgttatagac ttggcctaaa tccgcctcga gattatcgcc aatcgcaacc gcctgattga
    38281 ccgacagaaa ttgcagatca tcgccacggc actccacctg ccagccagcg gaaactaatg
    38341 cctgctcaac acggtcaatc acatcgcgaa aaggcatccg cagcggcggt aacgggcgcg
    38401 tgcctaaaat ttcctgatgc cccataaagg tatcgccacc ttcatgttgc agctctgcca
    38461 cgccccaggt tgcggaatct gacggctgca tatcgcctgg cgcataaccc aatgtgttga
    38521 ttagccccag cttctccagc gttggtagct gcaaatgcgg caactggctc aggatgtgac
    38581 cacaggtatt cgccccggca tcttgcgggc gcaccagcgt gacatctttc attgccccta
    38641 cgccaaagct atcaatcact aacaccacaa atcgcgccat ttatgcctcc agagggttgc
    38701 cgaggctgtc ataacgacca actatttctg gttcgccgcg ctgaataccg gacaccagca
    38761 ccacatcgct acgggtgaca aaaatctgcg tgcgaaaaca gagcaccact gcactgctca
    38821 ccggatactc gcctgccagc ggcagggtgt agtcgatact gctgtcatcc acagttttga
    38881 gattggtttc agtaatcttt tgattttctg gcgtaaacac cagcgcatgt tgcgcatgac
    38941 cacgacggta gtaaccgccg ccgtagcagt agctgtcgcc acggaaatga tgggagattt
    39001 cacttaacca gagcatcgcg atacgttcag gctgatcgcc ctgctggttt gccggaatag
    39061 taccggtcag cgcatgaccg ggttcggcat gagtcgcacc gtattccgcc agcaatggca
    39121 gcgaagtgca gctggtcgct gaaggtacgt tcagttgctc aatcgcaata ccagatttcg
    39181 ccagttgatc ccgtgcttgt accagcgtgt gaagattcgg tgttggcaaa actttcccag
    39241 cagcctcatc ccaaagcagg caagggaaat gggttagtcc ggctaaatgc agccccggca
    39301 gtttcttaat ttcagcaatc acctcgtgca gcgaatgctg tacaaaaccg ctctcctgcc
    39361 ccggataaag aaaatcatcg tcgctataaa ctttgagcag cacaggctgg acccgtccgg
    39421 ccttaaccgc tgccgcagaa acttcccgcg ctttgtcgag agtaaacacg gtgatgatgt
    39481 cggtgccctg ttcaacggcg tcagcaacct gatgacaagg gatttgtacc agatgcccct
    39541 gatgcgccac tggcaaacca gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca
    39601 caatgccgct gtagcctaat gccagcaatt tttccgccag ccacggatta cgaccaaatt
    39661 gcttggtcat cagatacagt tcaatgccgt aaagccgcgc cgtctcaatg agccgcttac
    39721 cgttttccag tatctgatcc acgtcgatca cccagctgtc cggggcgatt ttgccctgct
    39781 gccacaaact tagtgcggcg gaaatcagcg ccgggttctg gcgtttcaat gcttctacaa
    39841 acatcttgtt gcctcgtttc tgttccttga attaaatatt catttttttg aatatttagg
    39901 ttgtgcgacg tggatttgtc gggagcttca aacctcttcg ttagcagcca tccataatcc
    39961 ataaagattg gccagaaaat agccttcttc acacgggtta acttccagag cgaattcctt
    40021 caacaactcc tgatgcaatt gcacaacggc ttgccagtgg ctggattgcg ccagttccgc
    40081 cagcaactcg ttatccagcg gctctatttc ttcaccacgg cgactgcgca tcagtgcact
    40141 cgccatatgt gtcatcgcca tcgttccttg ctcactgcgc accggcagat ggcactcttt
    40201 ttccagtacg ttgacgacct gcatcatgcc tttgcagacg tccttatcaa taacgcctgc
    40261 ctcgcaaagc aggttgagtc tggtttccat atcagatgcc tttcgtttaa taactgggtt
    40321 cctgttcccc actcaccacc cgttgttgat gggcaagcag ggcgtgttta tctacaacag
    40381 cacgcagcaa ttgttgcatt gggtaatcat cgactcgcgt caggaccacg gcttcggtgg
    40441 cctgtaaaaa tcgcggatcg tctgttagcg gcgtcgcctc taatcccagc atggtcagtt
    40501 cgttttccgc caccacgttc cagatcaccg catcgacatc gcctttaaca atgcgttgta
    40561 aactctcgtg ataagagaga tcgactcgtt ccacatcact atcgccaaaa aagacatcgg
    40621 tcatgatttt ctgatccgcc gaacgactat cgagacctac tcgcttaaca tttccagact
    40681 cacctttgcg gcatatcagt tgatgttcac caacatacgt atgcgggcca agctcgaggg
    40741 cgatgcacaa attgttttgg ctgagataac tttccgccgc caggcgggaa actactgcca
    40801 tgtcatagac accgttaagt aaacactcca cgcgaatatc cgcgccgcgc atatgcgcat
    40861 agtaaaaagg aatgccatca aactgggctt tcaatccgct cgctaaacct tcgtacaagc
    40921 gggtataggg caaaggcatc gcacatacca cgttgttgat atccacatga ctcagcaatg
    40981 ctttgttatc catctcgaca agataactgc cattgcgccc acggcgttca atccgtatcg
    41041 ctccgcttga ttccagcgtt ttcaacgcgg cctgcgtcag gccaaccgat gaacggcatt
    41101 cagttgccag ctcatctatg gttttcagtc gattaccgca tttttcaccc aacaaataac
    41161 gggccagtgt cgtgatgacg acgccttctt ttttgataaa cgttcgacgc ataatatatt
    41221 ttcaataaaa tgaatattta tatcttcatt aaaatgaaga taagtggcga cgacgagatg
    41281 tggaaacggc gaggcgcttc acattttttc gggttgttgc gaaaagagga taaaaaaacc
    41341 gggtttcccc ggtttcagag tgatgacgaa gtgatttatt cgacaatgcg agcaaagggt
    41401 aattcccact ggcgcttgcc tgctcccctc accctaaccc tctccccaaa ggggcgaggg
    41461 gaccgtccga gcgttttgat attacggctt cgccacaaaa ccaatcgctt cgtacaccgc
    41521 tttcagcgta cgggaagcgt gtacactggc tttttctgcg ccgtctttca tcacctgttg
    41581 caggaaggct tcatcgttgc ggaaacggtg atagcgttcc tgcaattcag tcagcatacc
    41641 ggaaacggca tcagccactt cacctttcag atgaccatac atcttgcctt cgaactgttt
    41701 ttccagttct gggatgctct ggcccgttac cgcagaaagg atatccagca ggttggaaac
    41761 gcccgctttg ttctgcacat cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc
    41821 gcgtttgatt ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg
    41881 gttatcgtca gacttggaca tcttcttggt cggctccagc agcgacatta cgcgcgcgcc
    41941 agatttcgga ataaacggct ccggcacctt aaagatctcg ccatacagcg cgttgaaacg
    42001 ctgggcgata tcgcgactca gttccaggtg ctgtttctgg tcttcaccca ccggtaccag
    42061 attagtttga tacagcagga tgtccgcagc catcagcacc ggatagccaa acagaccagc
    42121 gttgatgttc tcggcataac gcgcagattt atctttaaac tgcgtcatgc gactcagttc
    42181 gccgaagtag gtatagcagt tcagtgccca gcctaactgc gcatgttctg gtacgtggga
    42241 ctgaacaaaa atggtgcttt tctcaggatc gataccacaa gccagataca aggccagcgt
    42301 atccaacgtc gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc
    42361 aacgatacag taaatgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc
    42421 catgtagtta ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt
    42481 agtcattttt cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat
    42541 ttatagactg ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttgtatc
    42601 cgtaggttaa gccaactgat gggcaacctg ccgcttttgc cgcctgaata tcattccgtg
    42661 agtcgccgac aaacagcatc tgttgtgggg caattcccat ccgctcagcc accagtaaca
    42721 gcgggtccgg atgcggtttt ttgttttgca catcatcgcc gccgataacc acgctaaagt
    42781 atttggcaat atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga
    42841 ccaagcctag cggcaggcct ttagcctgca acgcgcccaa cgtatcggca acgtgcggga
    42901 acaaaaacgt cccctcttcg gcaacctcgc catagtagcg atcgaacagt ttacgcagaa
    42961 tacgtacctg ttcttctgcc ggaatgtcgt catcaacggg cggtttcccc attgttttac
    43021 gcagagtcgc acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc
    43081 cgttaccaat ccaggtaata acgcgttctt cacctgcgac gggcaactcc agcgcataca
    43141 gcgccatatc taccgcagca gcaagaccag gagcactgtc gaccagcgta ccatcaagat
    43201 caaaagcgac gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg
    43261 cgcatttcat caatgacttt tttatagtct ggctggtcga agattgccga accggcgacg
    43321 aacatatccg cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc
    43381 acttccagac gaatgtcaaa gccagactcg tcgatacggc gacgtacttc gcgcagttta
    43441 tccagtgttt gaggaatgaa agactgaccg ccgaaaccag ggttgacgga catcagcagg
    43501 atcacatcca gcttatccat cacgtaatcc agatagctca gaggtgtcgc cgggttaaat
    43561 accagacccg ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg
    43621 gaggcttctg gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg
    43681 cgatcgacgg gtttcaccat cagatgtacg tcgatagggg cggtaatgcc gtagttacgc
    43741 aaggatttca gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg
    43801 tcaaaatgca cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg
    43861 gcaaaatcag ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg
    43921 agaattattt tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca
    43981 cctttttacg tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt
    44041 ttgcgcgctg ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa
    44101 tatggcgctc aaccagacct tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa
    44161 aactgtttgt gtgatacgcc gtaaagttgg cggtcgcaga cagcggtgca taaggcggat
    44221 cgcaatagac gacggatgca tcatctgcgc gctccatgct atcggcgtaa gactcacaat
    44281 agaaaaaggc attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg
    44341 gttttttgta gcggccaaac ggcacgttaa actcaccgcg cagattgtaa cgacacaggc
    44401 cgttgtaacc gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc
    44461 ttttgttgaa ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa
    44521 acagctcgcg tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac
    44581 tgatcaggtc gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca
    44641 ccgacccggc acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa
    44701 tatcatcaag caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca
    44761 tgccgattaa ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc
    44821 ttcaggcagc tcccgcaacg ataaaccatt gcgggagata accttgatta cttcagatcg
    44881 gcctgtacct gacgcagcgg tttcgcccac gggtttttgg cctgaacatc tgctggcaat
    44941 gtagataccg cttttttcgc ctcttctttc gaagcgtaca cgccagaaac caggacatac
    45001 cacggctgac cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc
    45061 gcccaaccgt tcaggttgtc gtagttagag gaactgctca gctgcagagt gtaatggctg
    45121 gacggtgccg atttcaacga accaacatta cctgcggtct tccctccagc ggctggtgtt
    45181 gccgtggttt gcgccgggga tgccgtctgt actggagccg tagtcgccgt ctcttttggt
    45241 gctggcgtag aagtcgcagc cggtgccttc gtgctcgcca caggggcagc tggttcaggt
    45301 tcagtacgct tcggcgtctg tgctaccggc ttcggctccg ttttcacggt tgcttgcggt
    45361 tttttcggtt caatcaccgc ctgctgacga actgggcgcg tagtggacgg acgttcagcg
    45421 gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc gaacaggcgc aaccgttgcg
    45481 ggttcagtcg gcaacgtgga attgaccgcc acattgttca gctgttgctg attttgtggc
    45541 tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc
    45601 accggggttt gcccttgagt cggcgtagaa gagatcggtg gcagagaaac atcctgctga
    45661 gtattttccg cagacgtggt tcccggcgct ggctgcacgc cattcgcctg atcggtcgca
    45721 ttgccagcaa gatcaatact cttctcgcca gacgcggttt gatcgctgga agtggtcgag
    45781 ggggctttta gcgcagaacc gataccgatg atcaacagca gtagaaccag aatgccgacg
    45841 cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgcgga
    45901 cgacgctcta cgcgctcttc atccacggtt tcatcttcgg attcgtcaat ttcttcttcg
    45961 atttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca
    46021 tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt
    46081 tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct
    46141 tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa
    46201 ttaacctgaa gctaatagac cgcttgataa gcggcctgac ctttcttgtt gttacgctga
    46261 ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact
    46321 cttaccaatt gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg
    46381 cagcatatgc ggtaaatacg cctgcgcgga catttcgcgc ggcccattga ccggtaaccc
    46441 agcccgcgtg agcagggtta taatacgctg cgtttcggca gaactaaact gcccgagacg
    46501 ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcttcaccat gtaaccaatt
    46561 gccatacccc atttcagctt caatggcatg accaaaggtg tgtcccagat tcagtaaagc
    46621 acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg
    46681 gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc
    46741 cagccagtta aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc
    46801 cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc
    46861 aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc
    46921 gccaacggag gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc
    46981 gcgctgataa ctcgccgccg cgaagccggt cagatcgccc actacgccgc cgccaagcgc
    47041 caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag
    47101 tacagctagg cttttatact gctcgccgtc agggaggata acgctatcga cgttaacacc
    47161 cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg tttcgctggt
    47221 gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa
    47281 accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat
    47341 aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag
    47401 ttgctttcca gcatgtgaat aatctggttt gcaaccactt tagcgctttg atcatcagta
    47461 cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc
    47521 agaacttcac gcggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg
    47581 cgtgcaagtt gcttttcgat ggtcgtttca agataaacga caacgccacg agcggaaaga
    47641 cggttacgcg tttcacggga tttcacagag ccgccgccag tagccagcac aataccctgt
    47701 ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct
    47761 aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg
    47821 taaaattcca tattgagttg ttgagctaac tggcgcccaa tagtgctttt tccggcaccc
    47881 ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta
    47941 ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac tgaaacctca
    48001 taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg
    48061 aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc
    48121 gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga
    48181 atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca aatcggtact
    48241 ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc ataaaaacat
    48301 taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa
    48361 ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc
    48421 gccaagcaac ggtacgctat cctgacccga tttattttta cgggtaaaaa tgccgcccag
    48481 cgccaacgtt tctccgcttt ttacctcgac ctgcgtttcg atctcctgct tatcaatcgc
    48541 cagcacttcg ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa
    48601 tttcagccgg atgcgacctt tttgtaacac cgtgggcgtg acctccatcc ccaggacggc
    48661 ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaaacctgat atggaatttc
    48721 gctctcctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactggcgat
    48781 aatatccagc tgctgttttt gttcgagcgc ggaaagctca agatccagca agcgtccgtt
    48841 gatgcgccca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctacc
    48901 aagcgtggtg acttgcccaa cgccaccagc gtgttgcgca tcggccagcg tccatttcac
    48961 gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg
    49021 cccgaccggc agatccattt gcgctaccca ctgttcaagc gcgcttaacg ccgttttgtt
    49081 atctcgtagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag
    49141 tagcttctcc cccgctttcg ccagttctcc cgcgtcggcg tattgcaggg ttatactgcg
    49201 attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcctcctggc gggcgatatt
    49261 gtcattctgc caggcaatgg aatgcactga gagaatgttg ccttcctgcc gcgttatcag
    49321 tccggcgctt ttcactacag tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa
    49381 cgacaccgta ccgctgacgt ctggcgacac gaccaggttc aacttctcct gttcagccag
    49441 cgcctgcaac acctgagcta ccggaacgtc atccaccatc agcgtcactt tttgcggctt
    49501 tgccgcctgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt gcttcatttg
    49561 tatctccttg ccgttgccac aaccattgtg gcggttcgca gtttgtcccg gtacccagcg
    49621 ttagtacgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca tcgttttgct
    49681 gcacccgtcg ccatttcttt tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc
    49741 ctaccatccc ctgatagcgc cactggctaa gttcgctaat ccggcatata gatcttccgg
    49801 cgatttaaaa gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca
    49861 gcgtttaacc ctcatgcggc atctccagtt gtagcgtgaa caaaagatca tcaccttcca
    49921 cgcttaacga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    49981 gcactgcttc ccacagcgtt ttcaacgcca actcacctcc ctgcgcggat ggatgccagg
    50041 aaaccagttg cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct
    50101 cgctgaaggg agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg
    50161 cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg
    50221 taactaacat cagcaaccag aatgcccagc aaaactggcg gaggcggggt gatgtggcga
    50281 accaccagtc aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc
    50341 cagcgtccct gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc
    50401 tggcggagtg acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc
    50461 tccagcgttc cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc
    50521 gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc
    50581 gaacgctgtt cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg
    50641 cgggcgagtt gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct
    50701 gtcagacgca gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc
    50761 aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    50821 ggattcatca gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccaccag
    50881 gcggcggtag cggcggctga cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc
    50941 cacatatcgc gacgctgtcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    51001 tcatccccac cgccagtttg cgcccccagc tatagcgcgt cgcccacagc cactgttcgt
    51061 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    51121 cactggcatc cggggtgatc gcactaacat gaacacgcaa cctttctgcc agcgtaagca
    51181 gcgttgccag ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt
    51241 cgctgtaatc gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg
    51301 acagccaggc cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct
    51361 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    51421 tccacggtaa caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa
    51481 tgtcgtgcgc cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca
    51541 cgatcgcaac cgctgccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa
    51601 atgtcattgt gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg
    51661 cttgccttta tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa
    51721 tttccagtga agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga
    51781 gcaggctcga tttatggcct ataccgctac atcgagccac aactgccgga tgtggtgaca
    51841 ttaaaagatg ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt
    51901 gctcaatacg gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg
    51961 gtgaaagcct ttatcgcgac ggaagacagc cgcttctacg agcatcacgg cgttgacccg
    52021 gtggggatct tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg
    52081 agtaccatta cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgatgatg
    52141 cgtaagatta aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa
    52201 atcctcgagc tttatctgaa caagatttac cttggttacc gcgcctatgg tgtcggtgct
    52261 gcggcacaag tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg
    52321 atagccgggt tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc
    52381 gtcgcgcggc gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccagcaa
    52441 cagttcgatc agacacgcac tgaggcgatt aacgctaatt atcacgcgcc ggagattgct
    52501 ttctctgcgc cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa
    52561 agtgcctatg aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc
    52621 gcgcagcagg cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc
    52681 ccggcaaatg tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat
    52741 acgctgaagg cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat
    52801 cctcagggag cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc
    52861 gttcgctggg cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg
    52921 accgatgttc tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg
    52981 ctggcacaag tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc
    53041 gttatggcgc tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag
    53101 gcactgcgtc aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa
    53161 ggtctgacgc tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc
    53221 ggttctgact ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt
    53281 caggggctgg gtcagtcgaa aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc
    53341 gactacgctg cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc
    53401 gaatcgctgg cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg
    53461 gtcatggcga acggcggctt cctagtggac ccgtggttta tcagcaaaat tgaaaacgat
    53521 cagggcggcg tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattcca
    53581 gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc
    53641 gctatctccc gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat
    53701 caggcgttag tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg
    53761 ctggcattcc tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag
    53821 ggtaccggct ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt
    53881 accactaaca gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg
    53941 gtctggattg gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg
    54001 attaaagatc agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac
    54061 gcttatatga aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt
    54121 attgtgacgg tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc
    54181 gaagagtatt tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg
    54241 accattatcg ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc
    54301 ttttcagttg gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg
    54361 gctaacaaaa tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg
    54421 tgcatcaggc aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg
    54481 aggaacagcg cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc
    54541 atatgcgcca gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat
    54601 tccgggtaga gatcttgcgc gaccacgata ttcattttgc tggaaaagta agacggtgcc
    54661 atgctgagct tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc
    54721 tcgcggttag cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct
    54781 aattcgtagg attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcgtccaca
    54841 atcggcacaa tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc
    54901 acgccattgc tgaactccag atccacgctc tcgacggtaa acagtcggga acgggctaca
    54961 gtttcaacat tcagaatggt gggtttttgt aatgatttgt tcatcgtggg atctatgctg
    55021 tgaaatcagc ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta
    55081 tttacattta tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt
    55141 gaaataactc caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt
    55201 tactgtgatg tgcttaatga tgctcaagtt aagctccacg cttgccgata gccaaccgca
    55261 gaatcatgta ttgtgtccgg tgcgactgac cacgcctgac agactaagta agatggggaa
    55321 agcatgagca ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga
    55381 tggctgataa aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat
    55441 gcgcaaacgc gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc
    55501 ctgacgcagg tattacaggt gcctggccca acggaagcta gcgcggcacc gatctctctg
    55561 gcgctgaatg ccgaaagcaa caacgtcatg atgctgacac acgctatcac acgttacggc
    55621 atctctaccg acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg
    55681 ccccccttct gggaacagta catcaacgat gagaataccg ttgaactgat tcataccgat
    55741 tcgctgccgt tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg
    55801 cgcggctatg ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt
    55861 gagcaaatcg agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt
    55921 ccgcgcgggc tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc
    55981 ctgattaccc cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc
    56041 gcaggtctgt gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt
    56101 ctgcgcggta caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac
    56161 aatatttcac tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct
    56221 caggatctcg gtcaacaaac cgatattgat atctatctcg accgccacgt agtgcgtcag
    56281 gggcgatatc tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc
    56341 agtacgatta tcgctgcggg ctcgctgcta gtgctgttta tgctgttatt ctggatcccg
    56401 ctggatatgc cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc
    56461 accagcgtaa aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc
    56521 ggtacgggaa tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct
    56581 tttttaccgt ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca
    56641 gaatctgaac tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac
    56701 cctaaaccgg aagatgaatc tcgcgtcagt gcctcattac gttcagcgat tcaaaaatcc
    56761 ggcatggtat tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct
    56821 gccaaagatg actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac
    56881 tgggacgcgc tggtaaaacg cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta
    56941 cgcccggtga gtgctgaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc
    57001 acgcatgaaa cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg
    57061 attgtcagcg acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac
    57121 gactacccgc cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat
    57181 acgccgttta acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag
    57241 catattggcc ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca
    57301 ttgctgctac tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt
    57361 taccagcgtc atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac
    57421 ccgcaactga tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat
    57481 gctaccctgt cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc
    57541 ctggcaggac gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt
    57601 cgataactat ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac
    57661 gccacaggaa gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa
    57721 ctggtactgt cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac
    57781 cgagatggga ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa
    57841 agccagcggt aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa
    57901 acttgagcat acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg
    57961 ttatccgaaa gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc
    58021 tgaaagaacg ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg
    58081 tattcgttac tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca
    58141 acgccatccg tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg
    58201 ccatgaaaga gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt
    58261 tttataaaac ccgcgcgctg gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg
    58321 ggcagcgcag caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg
    58381 gaaatgacga acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg
    58441 aggcagcctt gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga
    58501 tggcacgtaa acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag
    58561 agcgccgcga cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag
    58621 agatgattat gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc
    58681 gcggcgaact ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc
    58741 cgcagcccgt taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta
    58801 cgctgaagtt tgatggtgat atcaccgtac agctgcaggg cgacggtccg atgaatctgg
    58861 cggttattaa cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa
    58921 ttccagaaaa tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta
    58981 ccccgagcga aggcgaacgc tatcagggcg tagttggtct ggaaggtgat accctggcgg
    59041 cctgcctaga agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca
    59101 ccggcgacgt agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc
    59161 aaaataccca acaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg
    59221 aagaactgct gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg
    59281 tgacggttta cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg
    59341 ccgatgcgct gaaaacgctg cctgatgaag aagttgatag catcctggcg gaagatggcg
    59401 aaattgacat gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg
    59461 ctgaaatccg caacaacgcg tctccggcag atccgtaagt tcattaatga gtatgtccgg
    59521 cagagagggc tctgccggat tttacgactt aatggcctga tgtgctgcgc ttaccagcgc
    59581 ctatgaaata aatcgcaaat tattttggtc agatacagcg tcgtatccga taaaagcgtt
    59641 gtcacttact taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt
    59701 tcggcctcgt ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt
    59761 cgttcggaag taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat
    59821 agacaatgac tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat
    59881 tcagatcttg ctcgaccagt acgcaggcgt ctttttcaat acaggtcatc acgttatagc
    59941 gatcctggat taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta
    60001 acacattttc ggcgaatact ttttccgata cctgttcctg aagatgttgc tctccatcta
    60061 acgccatcag cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca
    60121 gcgactcaag cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct
    60181 ggtctggcgt gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca
    60241 tactgtccag caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg
    60301 caaggttcac tttgccctga agaacaaggg gattttgccc attacgccag acgatactga
    60361 caaaataccc cagcgaccac accagtaaca ccagctccgc cagcgcgccc tgcaagcggt
    60421 cgacagtcca gccgtactga gcgacccgca gccataaccc ccaggcggcg acgaacacat
    60481 acagcggagc aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa
    60541 gtgacgcttt ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg
    60601 ttaacagcaa cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca
    60661 gggtgatgat aaacatcagg gttagcaatg atactaacgg cagcaacccc gtggcgatta
    60721 gcgtgaacaa cttttgaata gagtcgatta aacgtgactg tgtacgcgcg aggatcaccg
    60781 ccagtgcggt aaccagacca aatgtaagat acataaacca gtcggttgca aaaaagagcg
    60841 tattgaaaaa cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc
    60901 acgtcaggcc attggagaga aaaatcacca gtaatataag tacgttatgc catactgact
    60961 ggtaaaagta gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg
    61021 gtagcaacaa cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag
    61081 ctctccaggg gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg
    61141 caataaacac cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga
    61201 agagtaaaac ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgtgat
    61261 tttttccggc aagccaggcg ataagcaggt agcaaaccag cccctgaagt aatccggtag
    61321 caatcatccc ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga
    61381 atcaaaaggt gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata
    61441 atcaaaaaaa gttagcgtgg tgaatcgata ctttaccggt tgaatttgca tcaatttcat
    61501 tcaggaatgc gattccactc acaatattcc cgccatataa accaagattt aaccttttga
    61561 gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga
    61621 atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc
    61681 gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag
    61741 tgaaatgcgc gttaacaatg gtttgccccc gcaagaactc gaggcttatg gtatcagtga
    61801 cgtacatgat atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc
    61861 gagcctgaca ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac
    61921 cgggatcttc accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg
    61981 cgatactttc tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc
    62041 ggaaacctgg cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt
    62101 cgttgtcgac gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac
    62161 cgaagtggcc tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga
    62221 gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca
    62281 gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat
    62341 gcagctgatt ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat
    62401 gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga
    62461 gaaaggcgat gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac
    62521 cgacccgaaa cgccgcctga ttggcgatga cgaacacggc tgggacgatg acggcgtgtt
    62581 taatttcgaa ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga
    62641 aatctacaac gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg
    62701 cactatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccaatcta
    62761 tcacatcgat aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt
    62821 cctgactgct gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac
    62881 ccagtatcac ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac
    62941 cgaaccgacg ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac
    63001 tcagtacgca gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt
    63061 taacactggc tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat
    63121 cgacgccatc ctcaacggtt cgctggataa tgcagaaacc ttcactctgc cgatgtttaa
    63181 cctggcgatc ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac
    63241 ctacgcttct ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga
    63301 caacttcgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa
    63361 actgtaatga tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga
    63421 ggcaccttcg cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg
    63481 gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca
    63541 tcccgttatg gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa
    63601 tggtgcgcgc actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg
    63661 caattcccgg accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc
    63721 tgactttgat ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc
    63781 gtttgatcga cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa
    63841 tttcccgctc atagccactt tcggcagcaa tcacctcacc gagcactgca ttaagatccg
    63901 ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg
    63961 cgttgcactc ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca
    64021 tcatctcagt cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg
    64081 ccatcagcag cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg
    64141 cacgggtaac ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc
    64201 ctttaccaac ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa
    64261 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    64321 gagagaaatc gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca
    64381 gccaggtttt cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    64441 ggccgcccag ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca
    64501 gacctgcctc ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct
    64561 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    64621 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    64681 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga
    64741 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    64801 agagccgtcc ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg
    64861 cgctgggtct tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    64921 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    64981 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
    65041 ctcatcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    65101 taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc
    65161 acgaatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
    65221 aatctccagg cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
    65281 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    65341 cattaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    65401 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    65461 acgcaggcgc atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac
    65521 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    65581 agctaaatct ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    65641 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    65701 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    65761 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    65821 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    65881 atcagtataa taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    65941 gcctggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa gtcttttttg
    66001 gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct
    66061 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    66121 tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgaact
    66181 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    66241 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    66301 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    66361 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    66421 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    66481 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca
    66541 tttccgagca aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca
    66601 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa
    66661 tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca
    66721 cgccagaagt cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag
    66781 ccgcgctgga tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc
    66841 tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg
    66901 gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca
    66961 cggtgccttc tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc
    67021 tttcgctgaa tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa
    67081 tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaaa
    67141 gcgtagtgag ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg
    67201 gtaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    67261 atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc
    67321 gtaccggggt aaaagtggcg gtggtcgatg ccactggcaa actggtggcg accgatacca
    67381 tttacccgca caccggacaa gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa
    67441 aacataacgt tgaactggtg gcgatcggca acggtacagc ttcccgcgaa actgaacgtt
    67501 tctatctcga cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg
    67561 aagctggcgc gtcggtttac tcagcttccg agctggcagc gcaggagttc ccggatctcg
    67621 acgtttcgct gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc
    67681 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    67741 cgcaactggc ccgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    67801 atctcaacac cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg
    67861 cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgc
    67921 tgaaagtcag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta
    67981 accacggtga taacccgctg gacgcctcta ccgttcatcc ggaagcctat ccggtggtgg
    68041 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    68101 tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg
    68161 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    68221 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg
    68281 cagtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc
    68341 tggttcacat ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag
    68401 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    68461 tgactatgcg tctggacgag cagcctggcg aaaccaactc tcgtcgcggc ggcggtaatg
    68521 aacgcccgca aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg
    68581 gtaatagcgc gatgatggat gcgctggcgg cagcgatggg taaaaaacgt taaacgcccg
    68641 gactggcccc taaacctgcg cgataaggcg ttcacgccgc atctggcaac gaacgctttg
    68701 cctgacataa aagtgccgga gaatatctcc ggcactttta ttccacagcc aaactcacaa
    68761 tatactccgt taatatttat cacttcatta acaactgaaa ccttaattaa acattagcca
    68821 gtccgggtaa ctcactattc gaattatatt ttcgctgcga tataaccttg agccacatca
    68881 acattgagtc agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag
    68941 aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca
    69001 tctggtctca tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa
    69061 gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga
    69121 aatcagcccg gcatatcgcc aaaaactgct ttctctaggc atgttacctg gctcctcttt
    69181 tgatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag
    69241 cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata
    69301 cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca aattctggca
    69361 agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg
    69421 ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg
    69481 acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa
    69541 tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt
    69601 ctaaccttga gcgtaacctg tacctgacgc tgcaactgct ggaactcggc attccctgca
    69661 tcgtggcgct gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg
    69721 ctctatcggc acgtcttggc tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta
    69781 ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc
    69841 attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca
    69901 tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc
    69961 gcgcctacgc cggtgaagcg tcgcagcatc tggatgccac cctcgcccgt ctgcgtaatg
    70021 aaatggatga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct
    70081 gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata
    70141 aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt
    70201 tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttaacgtc ggctccgtgg
    70261 cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga
    70321 ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc
    70381 agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat atggcgcgtg
    70441 cagcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc
    70501 cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg
    70561 caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc
    70621 tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcc ctggcggttt
    70681 tctcgctgta tatgctcggt atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca
    70741 ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac catgtaccac
    70801 acgttaaaag cctgattatc cagacctggc agcgtctgaa gggcttcgtt ctgcgtgctg
    70861 gtaaagtgat tatcatcgtc agcattttcc tgagcgcttt caacagcttc tcgttgagcg
    70921 ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgataaccc
    70981 ctgtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta
    71041 cgggcgcaat ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa
    71101 atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg
    71161 cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc
    71221 ccattgaagc tagcaaaggc aacggtgaaa tgggtaccgg ggcgatgggc gtgatggatc
    71281 agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac
    71341 catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct
    71401 ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca
    71461 gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggtaatc ctgtttaaca
    71521 tcgtagctat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa
    71581 cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc
    71641 ttcacttatt caggtacgtg atttgctggc gttacggggc cgtatggaag cgacccagat
    71701 aagccagaca ttgaacactc cgcagccaat gattaacgcc atgctgcaac agctggaaag
    71761 catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa
    71821 aagctgcccg gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc
    71881 catcgcctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt
    71941 tccataaaaa tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt ctcgcaggga
    72001 tgaaaactcg ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca
    72061 ggtgaaggaa cgccatgagt aaaaagcaga gttccacccc acatgatgcg ctgttcaaac
    72121 tctttttacg ccaaccggag acggcacgcg attttcttgc gtttcattta ccggcaccca
    72181 ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gaggagcttt attgatgacg
    72241 atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat
    72301 acatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga
    72361 tgcgttacgc tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga
    72421 tggtggtgcc attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt
    72481 ggctggattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc
    72541 tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc
    72601 tggagttaat tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg
    72661 cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca
    72721 tactgcaaac tggcgacgct gtacgtttta acgattttat cgacggcgtt gccgaacgtt
    72781 caccgaaaca caaggagagt ttaatgacta ttgcggaaag attgcggcag gagggggaac
    72841 aatccaaagc cctgcatata gccaaaataa tgcttgaatc cggagtccct cttgcagaca
    72901 tcatgcgatt taccgggctg tcagaagaag ag
//
LOCUS       DAJTOW010000005        72040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTOW010000005 DAJTOW010000000
VERSION     DAJTOW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.005"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1306..2901)
                     /locus_tag="OO244_000388"
     CDS             complement(1306..2901)
                     /locus_tag="OO244_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5662337.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3256..5427)
                     /gene="glcB"
                     /locus_tag="OO244_000389"
     CDS             complement(3256..5427)
                     /gene="glcB"
                     /locus_tag="OO244_000389"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5662338.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5449..5853)
                     /locus_tag="OO244_000390"
     CDS             complement(5449..5853)
                     /locus_tag="OO244_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5662339.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5858..7077)
                     /gene="glcF"
                     /locus_tag="OO244_000391"
                     /pseudo
     CDS             complement(5858..7077)
                     /gene="glcF"
                     /locus_tag="OO244_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7088..8140)
                     /gene="glcE"
                     /locus_tag="OO244_000392"
     CDS             complement(7088..8140)
                     /gene="glcE"
                     /locus_tag="OO244_000392"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5662340.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8140..9639)
                     /gene="glcD"
                     /locus_tag="OO244_000393"
                     /pseudo
     CDS             complement(8140..9639)
                     /gene="glcD"
                     /locus_tag="OO244_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9890..10660
                     /gene="glcC"
                     /locus_tag="OO244_000394"
     CDS             9890..10660
                     /gene="glcC"
                     /locus_tag="OO244_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5662341.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10784..11925)
                     /locus_tag="OO244_000395"
                     /pseudo
     CDS             complement(10784..11925)
                     /locus_tag="OO244_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12287..14017
                     /locus_tag="OO244_000396"
     CDS             12287..14017
                     /locus_tag="OO244_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5662342.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14014..14928
                     /locus_tag="OO244_000397"
     CDS             14014..14928
                     /locus_tag="OO244_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5662343.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14961..15209
                     /locus_tag="OO244_000398"
     CDS             14961..15209
                     /locus_tag="OO244_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5662344.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15209..16380
                     /locus_tag="OO244_000399"
                     /pseudo
     CDS             15209..16380
                     /locus_tag="OO244_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16416..17495)
                     /gene="lptG"
                     /locus_tag="OO244_000400"
     CDS             complement(16416..17495)
                     /gene="lptG"
                     /locus_tag="OO244_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5662345.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17492..18562)
                     /gene="lptF"
                     /locus_tag="OO244_000401"
     CDS             complement(17492..18562)
                     /gene="lptF"
                     /locus_tag="OO244_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5662346.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18593..19153)
                     /locus_tag="OO244_000402"
     CDS             complement(18593..19153)
                     /locus_tag="OO244_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5662347.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19165..19989)
                     /locus_tag="OO244_000403"
     CDS             complement(19165..19989)
                     /locus_tag="OO244_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662348.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19989..21338)
                     /locus_tag="OO244_000404"
     CDS             complement(19989..21338)
                     /locus_tag="OO244_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5662349.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTLFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21384..22142)
                     /locus_tag="OO244_000405"
     CDS             complement(21384..22142)
                     /locus_tag="OO244_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662350.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22174..22887)
                     /locus_tag="OO244_000406"
     CDS             complement(22174..22887)
                     /locus_tag="OO244_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5662351.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23061..23753
                     /locus_tag="OO244_000407"
     CDS             23061..23753
                     /locus_tag="OO244_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5662352.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23802..25301)
                     /gene="pitB"
                     /locus_tag="OO244_000408"
     CDS             complement(23802..25301)
                     /gene="pitB"
                     /locus_tag="OO244_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5662353.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25593..27452)
                     /gene="gss"
                     /locus_tag="OO244_000409"
     CDS             complement(25593..27452)
                     /gene="gss"
                     /locus_tag="OO244_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5662354.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27657..28523
                     /gene="yghU"
                     /locus_tag="OO244_000410"
     CDS             27657..28523
                     /gene="yghU"
                     /locus_tag="OO244_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5662355.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28646..28894)
                     /gene="hybG"
                     /locus_tag="OO244_000411"
     CDS             complement(28646..28894)
                     /gene="hybG"
                     /locus_tag="OO244_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5662356.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29028..29369)
                     /gene="hypA"
                     /locus_tag="OO244_000412"
     CDS             complement(29028..29369)
                     /gene="hypA"
                     /locus_tag="OO244_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5662357.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29362..29850)
                     /gene="hybE"
                     /locus_tag="OO244_000413"
     CDS             complement(29362..29850)
                     /gene="hybE"
                     /locus_tag="OO244_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5662358.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29843..30337)
                     /locus_tag="OO244_000414"
     CDS             complement(29843..30337)
                     /locus_tag="OO244_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5662359.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30337..32040)
                     /gene="hybC"
                     /locus_tag="OO244_000415"
     CDS             complement(30337..32040)
                     /gene="hybC"
                     /locus_tag="OO244_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5662360.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32037..33215)
                     /gene="hybB"
                     /locus_tag="OO244_000416"
     CDS             complement(32037..33215)
                     /gene="hybB"
                     /locus_tag="OO244_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5662361.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33205..34191)
                     /gene="hybA"
                     /locus_tag="OO244_000417"
     CDS             complement(33205..34191)
                     /gene="hybA"
                     /locus_tag="OO244_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5662362.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34194..35312)
                     /gene="hybO"
                     /locus_tag="OO244_000418"
     CDS             complement(34194..35312)
                     /gene="hybO"
                     /locus_tag="OO244_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5662363.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35501..35788)
                     /locus_tag="OO244_000419"
     CDS             complement(35501..35788)
                     /locus_tag="OO244_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5662364.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35907..36794)
                     /locus_tag="OO244_000420"
     CDS             complement(35907..36794)
                     /locus_tag="OO244_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5662365.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36951..37991
                     /gene="gpr"
                     /locus_tag="OO244_000421"
     CDS             36951..37991
                     /gene="gpr"
                     /locus_tag="OO244_000421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5662366.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38031..38525)
                     /locus_tag="OO244_000422"
     CDS             complement(38031..38525)
                     /locus_tag="OO244_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5662367.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38716..39600
                     /gene="yghA"
                     /locus_tag="OO244_000423"
     CDS             38716..39600
                     /gene="yghA"
                     /locus_tag="OO244_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5662368.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39872..40297)
                     /gene="exbD"
                     /locus_tag="OO244_000424"
     CDS             complement(39872..40297)
                     /gene="exbD"
                     /locus_tag="OO244_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5662369.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40304..41038)
                     /gene="exbB"
                     /locus_tag="OO244_000425"
     CDS             complement(40304..41038)
                     /gene="exbB"
                     /locus_tag="OO244_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5662370.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41290..42477
                     /gene="metC"
                     /locus_tag="OO244_000426"
     CDS             41290..42477
                     /gene="metC"
                     /locus_tag="OO244_000426"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5662371.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42617..43276
                     /locus_tag="OO244_000427"
     CDS             42617..43276
                     /locus_tag="OO244_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5662372.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43316..44215)
                     /gene="yqhC"
                     /locus_tag="OO244_000428"
     CDS             complement(43316..44215)
                     /gene="yqhC"
                     /locus_tag="OO244_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5662373.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44409..45572
                     /gene="yqhD"
                     /locus_tag="OO244_000429"
     CDS             44409..45572
                     /gene="yqhD"
                     /locus_tag="OO244_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5662374.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45677..46504
                     /gene="dkgA"
                     /locus_tag="OO244_000430"
     CDS             45677..46504
                     /gene="dkgA"
                     /locus_tag="OO244_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5662375.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46704..47630
                     /locus_tag="OO244_000431"
     CDS             46704..47630
                     /locus_tag="OO244_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5662376.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47681..47938
                     /gene="yqhH"
                     /locus_tag="OO244_000432"
     CDS             47681..47938
                     /gene="yqhH"
                     /locus_tag="OO244_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5662377.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47981..50200)
                     /locus_tag="OO244_000433"
     CDS             complement(47981..50200)
                     /locus_tag="OO244_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5662378.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50311..51723)
                     /gene="ftsP"
                     /locus_tag="OO244_000434"
     CDS             complement(50311..51723)
                     /gene="ftsP"
                     /locus_tag="OO244_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5662379.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51798..52535)
                     /gene="plsC"
                     /locus_tag="OO244_000435"
                     /gene_synonym="parF"
     CDS             complement(51798..52535)
                     /gene="plsC"
                     /locus_tag="OO244_000435"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5662380.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52769..55027)
                     /gene="parC"
                     /locus_tag="OO244_000436"
     CDS             complement(52769..55027)
                     /gene="parC"
                     /locus_tag="OO244_000436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5662381.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55573..56055)
                     /locus_tag="OO244_000437"
     CDS             complement(55573..56055)
                     /locus_tag="OO244_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5662382.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56108..56500)
                     /gene="ygiW"
                     /locus_tag="OO244_000438"
     CDS             complement(56108..56500)
                     /gene="ygiW"
                     /locus_tag="OO244_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5662383.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56652..57311
                     /gene="qseB"
                     /locus_tag="OO244_000439"
     CDS             56652..57311
                     /gene="qseB"
                     /locus_tag="OO244_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5662384.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57308..58582
                     /gene="qseC"
                     /locus_tag="OO244_000440"
     CDS             57308..58582
                     /gene="qseC"
                     /locus_tag="OO244_000440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5662385.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58628..58960)
                     /locus_tag="OO244_000441"
     CDS             complement(58628..58960)
                     /locus_tag="OO244_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5662386.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59279..59860
                     /gene="mdaB"
                     /locus_tag="OO244_000442"
     CDS             59279..59860
                     /gene="mdaB"
                     /locus_tag="OO244_000442"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5662387.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59891..60205
                     /locus_tag="OO244_000443"
     CDS             59891..60205
                     /locus_tag="OO244_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5662388.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60253..62145)
                     /gene="parE"
                     /locus_tag="OO244_000444"
     CDS             complement(60253..62145)
                     /gene="parE"
                     /locus_tag="OO244_000444"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5662389.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62174..62755)
                     /gene="yqiA"
                     /locus_tag="OO244_000445"
     CDS             complement(62174..62755)
                     /gene="yqiA"
                     /locus_tag="OO244_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5662390.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62755..63582)
                     /gene="cpdA"
                     /locus_tag="OO244_000446"
     CDS             complement(62755..63582)
                     /gene="cpdA"
                     /locus_tag="OO244_000446"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5662391.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63607..64029)
                     /locus_tag="OO244_000447"
     CDS             complement(63607..64029)
                     /locus_tag="OO244_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5662392.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64030..64659)
                     /gene="nudF"
                     /locus_tag="OO244_000448"
     CDS             complement(64030..64659)
                     /gene="nudF"
                     /locus_tag="OO244_000448"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5662393.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64864..66345
                     /gene="tolC"
                     /locus_tag="OO244_000449"
     CDS             64864..66345
                     /gene="tolC"
                     /locus_tag="OO244_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5662394.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66493..67164
                     /locus_tag="OO244_000450"
     CDS             66493..67164
                     /locus_tag="OO244_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5662395.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADNCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67170..68330
                     /locus_tag="OO244_000451"
     CDS             67170..68330
                     /locus_tag="OO244_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5662396.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68368..69156)
                     /gene="ygiD"
                     /locus_tag="OO244_000452"
     CDS             complement(68368..69156)
                     /gene="ygiD"
                     /locus_tag="OO244_000452"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5662397.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69299..70072
                     /gene="zupT"
                     /locus_tag="OO244_000453"
     CDS             69299..70072
                     /gene="zupT"
                     /locus_tag="OO244_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5662398.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70130..70300)
                     /locus_tag="OO244_000454"
     CDS             complement(70130..70300)
                     /locus_tag="OO244_000454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662399.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70561..71214)
                     /gene="ribB"
                     /locus_tag="OO244_000455"
     CDS             complement(70561..71214)
                     /gene="ribB"
                     /locus_tag="OO244_000455"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5662400.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71318..71466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71588..71878
                     /gene="ubiK"
                     /locus_tag="OO244_000456"
     CDS             71588..71878
                     /gene="ubiK"
                     /locus_tag="OO244_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5662401.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18017 a        19165 c        17737 g        17121 t
ORIGIN      
        1 ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt acagggctta
       61 ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca taagaaatca
      121 aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt cattaatact
      181 gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga gatccatcgg
      241 tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg tatcagattc
      301 ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat ttacattatt
      361 tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt aatctcttac
      421 tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctattttta gcaggaaata
      481 ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataagttaa tgattttaat
      541 gattttaatg attttttcat ttattcacac ctaataaaag tattcaaatt cagatgattt
      601 ttctgctcaa cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt
      661 acgatttaat gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa
      721 aatgattggt gtgttgttgg agttaaaata catacataat attaacatct gccaacattt
      781 cactttccgc aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct
      841 ttattaatag gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat
      901 cttttatgta acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt
      961 tctcattatt ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa
     1021 ttttgatcta ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca
     1081 tgaaagcaac agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta
     1141 ctttataacc agcacagaag ccaagttgta taaatattaa acaattcgca tcgtaaaacg
     1201 gagactacgt ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac
     1261 aaatggaaag ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat
     1321 cccggtaaac acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag
     1381 actgtgcttc acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc
     1441 gcaggccacg gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt
     1501 tgctgccacc agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga
     1561 accaaacagg gcgtttgaag aagtgtccga cccggtaagg aatacgccca gccagccgag
     1621 aaacggtgag aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt
     1681 ggacatgccg gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat
     1741 cggccacttc agactaatta gcatttcggc aaagacgcca atccctttct tgatgcccac
     1801 gccgaggatg aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag
     1861 agggtcgaat ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt
     1921 caacacttgt tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc
     1981 gcccggagca aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa
     2041 gattaaaaac ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg
     2101 accgccagaa gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc
     2161 cgtttcggta tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac
     2221 cagcgccgaa gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt
     2281 gacagcgaag cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca
     2341 gccgtccatc attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg
     2401 tcccgccatt gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat
     2461 cggcacgccc aacgcaccaa acgccaccgt cgcaccaaag ccagcagctc cttccagcaa
     2521 cgcaccaaag gagaaaccaa tcagtaacac ctgcaaacgc tgatcgtcgg tgatggagat
     2581 aaccgagctg cggataatat cgaactgccc gctggcaacg gttaatttat acaggaacac
     2641 cgccgcgaca ataatccacg caattggcca taatccataa ataaagccat agcccgcagc
     2701 agcaaatgcc atatcaatcg gcattttaaa ggcgaatatc gcaatcagga tagataatat
     2761 aagggttatt gctccagcaa catgtccttt cagacgtaat accgcgagtg caacgaagaa
     2821 gaatattatc gggatcaggg cgaccagagc ggatagcccc agtcctccca tcggcatata
     2881 catttgggtc caggtaacca tattgttcag tctcttatta tctttatatg cttgatatac
     2941 ttaaggttgt aataagcaaa agaggactga actgtaaaat ataggcgtta tactttacag
     3001 caacagtacg ccgctaacgc aattgctacc tctggcataa caagtatatc gggtaagggt
     3061 ttctgttccg cacacgcagg cgcagagtat cgttaagatg tccatattgt tgttttaggc
     3121 ccgctagtaa tgcgctacgg gtatttaata ttgttaaacc ctgataatcg ctccggttat
     3181 ttccgggata aatgtactac cgcagttact atcatagccc cgacaataaa acttgccggg
     3241 gcttttttga cgctattaat gactttcttt ttcgcgtaaa cgccaggcgt gtaataacgg
     3301 ttcggtataa ccgtttggct gtttcacgcc gaggaagatt aaatcgctgg cagctttaaa
     3361 agcacacgag ttagcgaaat tccccgccat cggacgataa gccggatcgc cagcattttg
     3421 ctgatcaacc actttcgcca tattctccag cgacgcctgc acctgttctt tggtcagaat
     3481 accgtgacgt aaccagttgg cgatatgctg gctggagata cgcagcgttg cgcggtcttc
     3541 catcagcgcc acgttgtgaa tatccggcac ttttgaacaa ccaatcccct gctccaccca
     3601 acgcaccacg taccccagaa tcccctgcac gttgttatcc agctcttgtt ggatctcttg
     3661 cgccgaccag ttagcgtttt cagccaccgg aatagtcagc agatcgtcca gcagcggttc
     3721 aaattcagca ctgaactcgg tctgggcaat gttggcttgc acgctctgta cgttggtatg
     3781 gtggtagtgc agcgcatgga gcgtagcagc ggttggtgac ggaacccagg ctgtgtttgc
     3841 cccggcacgc agttggtcgc ccttctggct gtacatgtct gccatcaggt ccggcattgc
     3901 ccacatgcct ttaccgattt gcgctttacc gcgcagccca cagaacagac cggaaagcac
     3961 gttattacgt tcgtaggctt tgatccacgg cgtcgatttc atctggtttt tacgcagcat
     4021 cgggccagct tccatcaccg aatgcatttc atcgccggta cggtcgagga aaccggtatt
     4081 gatgaacgcc acgcggttgc gcgcctgagc gatacagcta cgcaagttca gcgaggtccg
     4141 gcgttcttca tccataatgc ccattttcag ggtattcggt gccataccga gcattgtctc
     4201 aatgcgggta aacagtttgt tggcgaacgc cacttcctgc ggaccgtgca ttttcggttt
     4261 cacaatatag acgctgccag tgcgcgagtt tttctgcact tttaaatcat agagggcaat
     4321 cgcgccagtc atgacgccat caagaatacc ttccgggatt tcattgcctt cgctgtccca
     4381 aatcacagga atggtcatca aatgacccac gttgcggata aacagcaacg agcgtccgtg
     4441 cagagaaatt tcagagccat cggcggcggt gtactgacga tcgtcattca gtttacgtac
     4501 gatttgccga ccgtttttct ccattttctc ttgcagagtc ccctgcatca ggcccagcag
     4561 attgcggtac agcaggattt tatcttccgc atcaaccgcc gcgaccgaat cttcgcagtc
     4621 gagaatggta ctgatagcag cttcgacgat aacatcgttg atgtgcgccg gatcgtcttt
     4681 gccaatccgc ccgttggcat caatttgcag ttcaatatgc aggccgttat ttttcagcaa
     4741 aatgcaggtc ggcgcagcgg catcgccacg gtaaccgaca aactgtgctg gagtacgtaa
     4801 cgtggtttct ttaccatttt tcaactggat gcgtaattgt ttatcgacca ccttaaacgc
     4861 caccacatcc tgatagctgc cgttttccag cggtagtgat tcatcgagga aacgtcgaac
     4921 ccaggcgata acctgctcac cgcgctgcgg atcgtagcca ctgaccatcg ccccttcctg
     4981 cgggatgatg tcgctgccgt ataacgcatc gtacagtgag ccccagcgag cgttcgccgc
     5041 gttcagcgcg tagcgggcgt tcattgccgg aaccaccaac tgtggccccg cctgactggt
     5101 gatttcgctg tcaatgcccg tggtttccac cgtcacgcgc tccggttgcg gcaccaggta
     5161 gcccagttca cgaaggaaag atttataggc cgctttatct tttaccggcc ccggattgct
     5221 gcgatgccac tcatcaagcg ccgcctgaat gcgatcgcgt tctgccagca actgacgatt
     5281 ttctggtgcc agatcgtgaa cgatctcatc aacattgtgc cagaatgcag cagcgtccag
     5341 ttccacgccc ggcaaaactt ctccatccac aaaacgttta aaattggcgt caatgcgtaa
     5401 acggccctgg gttatggttt gactcattgt ttctctcctc gttttcgctt atttcgccaa
     5461 caccgctgct gccactttcg cgacctgcgc atcctgtgct ccggttaaac cagaaacgcc
     5521 cacggcacca ataatttgcc catcaacaac aaccggtacg ccgccttcca gcgacgtcag
     5581 taacggcgca gtcacgaacg cggtacgtcc gttgttcacc atctcttcat agcccttagt
     5641 ttcacgacgc cccagcgcgg cggtacgcgc tttctcctgg gagatgtaag ccgcaatcgg
     5701 cgcgcaatcg tccatgcgac ttaacgccag cagatgaccg ccgtcatcgg caacagcaat
     5761 ggaaacagac cagttatttt tctgcgcctc ttcctgacct gcggcaataa ttgcactcgc
     5821 catttgctgg ctaagaatga ctttagtttt cattttttta ttccttttca agggcttgtt
     5881 ctacaatttc aatccagtga cgcacagagg tacgaccggc gctcgccaga tgcgtctggc
     5941 aaccaatgtt ggcggtgacg atcatttccg gtttgccgct ttccagcgca ttcattttgt
     6001 tatcccgcag ctggcgtgcc agatcgggat gcgttaacgc atatgttccc gctgaaccgc
     6061 agcacagatg gctgtcggga acgtccgtta aggtaaatcc aagacgaagt aacacttttt
     6121 ccacttcgcc gttcagcttt tgcgcatgtt gtagggtaca cgggcagtgg aaggccagtt
     6181 ttttatcgcc gcgaattgcc agtttttcca gcggttcctc gcgcagaagt tcgactaaat
     6241 cgaccgccag ttcactgacc tgacgcgctt tatcggcata taacgcatcg tttttcagca
     6301 tctgcccata ctctttgacg aacgcgccgc aaccgctggc ggtttgcaaa attgcctcgg
     6361 cacctgcttc aatcgcgggc caccaggcat caatattatt gcgcgcccgt gccaaccctt
     6421 tctcctgcgc attaagatga tagtccaccg cgccacaaca gcctgcttcg ttagctgaca
     6481 tgacgctgat ccccagacga tccagcactc gcgcagttgc cgcgttggtg ttgggcgaaa
     6541 gcgtaggctg ggcgcagcct tccagcatta aaacccgacg cttatggcgc agcggcggac
     6601 gcggtttagc tttcaccgtt tcagcaggca gttttgctct gacctgttcc ggtaaaaacg
     6661 gtcgcagcac cagccctacc tgcgtcagcg cacggaagac cgccggacgc ggcactacct
     6721 ggcgcaatcc ttcgcgcagc attcgctccg gcagtgggcg tttcactttc tgctcgacaa
     6781 tatcacgccc gatatccagc aaattgtgat agcgcacacc agaaggacag gtggtttcac
     6841 aattgcggca agtgaggcag cgatcgagat gctcctgtgt tttaagcgtg acttcgttgc
     6901 cttccagcac ctgtttaatc agatagatgc gcccgcgcgg cccgtccagt tcatcgccca
     6961 gaagctgata ggttgggcag gttgcggtac aaaatccgca gtgaacacag gcgcacagga
     7021 tgctgtcggc ttccagcgcg cgttctgccg catctcttcg gttaatcggg tttgcatagc
     7081 ctgctcctca aagttccgcg tacatgcgac cggggttaaa cacgccgcaa gggtcgagct
     7141 gctgtttaag ctgctggtga tagcggaata aaggagccga tagcggggca aagccaccat
     7201 ctccggcact aaagcgggtc gcatgaccgc cagcgttgcg ggcgatgcga tggatttgat
     7261 tgtcatcggc tgtcgatttc agccagcgta acgccccgcc ccagtcgatc agttgctcgc
     7321 cgggtaaatc catcatcggc gcatcgctgg gtaatgaaat gcgccataag gtacctggta
     7381 acgagaagaa cggcagttgt tgttcacgca attgctgcca gaactgaccc acaacctctt
     7441 cgccacccag cagttcacgc gctgctttta ccgatccttc gccgccctca aggcggatcc
     7501 acaacgcatt gtcgaagtaa cataagccac taatgggtaa tggctggagt tgccactcgg
     7561 cgatttcact catggcttct tgcaggctga tttcccgacg caggctcaga gaggcacgcg
     7621 gtcgcggtaa cactttcatt gagatttcag tgagcacgcc aagacaaccg tagcttccgg
     7681 ccattaaccg tgagagatcg tatccggcaa cgtttttcat cacttcgcca ccaaaacgca
     7741 gatgttttcc agtgccggta atgatgcgcg tgccgaggac aaaatcgcgg accgaaccgc
     7801 tccacgggcg acgcggcccc gccagcccgc aggcgaccat cccgccccag gtggcttctt
     7861 caccataatg cggcggctcg cagggaagca tttgcccggc gctctccagc gccgcttcaa
     7921 tcgccaccag cggcgttccg gcacgagcgg ttatcaccag ctcggtcggg tcgtaattaa
     7981 caatgccgcg atgacaacgc acatccagcg tttgcccggt gactgggcga cctaaaaagg
     8041 ctttgctatt gctgccctga atcaccagcg gcgttttatc gctaattgcc tgattcacct
     8101 gctccagcag cgcctgactg taatcacact cgcgtagcat caaaagcgct ccagttcagg
     8161 aaaaggtaaa tgaccgtgat gcacatgcat ggcaccaaat tcagcacagc ggtgtagcgt
     8221 gggaatgttt ttcccagggt tcagcaaacc atcggggtca aacgccgcct tgaccgcatg
     8281 gaaggtcgtg atttcatcgc tgttgaactg ggcgcacatt tgattgattt tttctcgccc
     8341 gatgccatgt tccccactga tgctgccgcc aacttcaacg cagagttcga ggatcttccc
     8401 gcccagctct tccgcgcggg caaattcacc aggttcgttg gcatcgaaaa ggattaacgg
     8461 gtgcatgttg ccatctccgg catgaaagac gttggcaaca cgtaaatcat attgctgcga
     8521 taaacgggca atgccttcca gtacgccagg cagggcgcga cgcgggatgg tgccatccat
     8581 gcagtagtaa tccggggaga tacgtcctac cgccgggaac gcatttttgc gaccggccca
     8641 gaaacgtacg cgctctgctt cgtcctgtgc caaacggacg tcagtcgcgc ccgctttcaa
     8701 caagatgtag ttaacccgct cgcagtcttc ctgtacgtca gactccacgc cgtccagttc
     8761 gcataacaaa atcgcttcgg cgtcgacggg ataaccggca tgaataaaat cttccgccgc
     8821 gcggatcgac aggttatcca tcatctccag cccgccgggg ataatgccat tggcgatgat
     8881 gtcaccaacc gcaagtccgg ctttttctac cgagtcaaag ctggctaaca gaacccgcgc
     8941 cacgggcggc ttcggcagca gttttaccgt cacttcggtg gtcacgccga gcataccttc
     9001 cgatccggtg aacagcgcca gcaggtcaaa accaggtgaa tccagcgcgt ccgatcccag
     9061 cgtcagcgcc tcgccgtcca gcgtttgcac ttcaattttc agcaggttat gtacggtcag
     9121 accatatttc aggcagtgga cgccaccggc attttccgcc acattgccgc caattgaaca
     9181 ggcgatttgt gaggaaggat ccggcgcgta atagagatta tgcggcgcaa cggcctagga
     9241 gatcgccagg ttacatacgc ccggctgcac gcgcgcgcgg cgaccaacgg ggttaatgtc
     9301 gaggctctct ttaaagcgcg ccatcaccaa caacacacct ttttccagcg gcagcgcgcc
     9361 accagaaagc ccggtgcctg caccacgggt caccaccggt acacgcaggc gatggcagac
     9421 agccagaatc gctgtcacct gttccatttg cttaggcaga acaaccagta atggacgcgt
     9481 gcgatacgcg ctcaacccgt cacactcgta aggaatgatc tcctcatcgg tatgcaggat
     9541 ctcaagtcca gggacatgct cacgcagtgc catcagtacc gatgtgcggt cgacatcggg
     9601 taaagcgcca tcaagacgct cttcgtacaa gatgctcatg agtaggcttc gctttgttgt
     9661 gttgtgtggc agctgatttt tgcgcgctgc ttctgtgaac agttattaag cgggcttttc
     9721 gttttcgtct atctctttag ctaccggtca gaccattttt tttccagctc tgtgaccttg
     9781 tcttggttaa cttaatgtta aattgatgta acataatcac ttgcgtgatg tgcgtgtttt
     9841 gcgagttaag aacagaaaaa ttggtcctac ctgtgcacga ggtccgggaa tgaaagatga
     9901 acgtcgccct atttgcgaag tggttgcaga gagtatcgaa cggttaatta tcgacggcgt
     9961 actgaaggtc ggtcagccgc ttccctcgga acgtcgactg tgtgaaaagc tcggcttctc
    10021 acgctccgca ctgcgtgaag ggctgaccgt gctgcgcggg cgcgggatta ttgaaacggc
    10081 gcagggtcgc gattctcatg tcgcacggct taatcgggag caggacacca gcccgctgat
    10141 ccatctgttc agtacgcagc cgcgaacgct gtacgatctg ctcgacgttc gcgcattact
    10201 ggagggcgaa tcggcaaggc tggcggcaac gctgggaacg caggctgatt ttgttgtgat
    10261 aacccgctgt tatgaaaaaa tgctcgccgc cagtgagaac cacaaagaga tttcgctgat
    10321 cgaacatgcg cagttggatc acgctttcca tctcgccatt tgtcaggctt ctcacaatca
    10381 ggtgctggtg tttacgctgc aatcattgac cgatctgatg tttaattcag tgtttgccag
    10441 cgtaaataat ctctaccatc gaccacagca aaaaaagcag atcgatcgcc agcatgcgcg
    10501 gatctacaac gcggtgttgc agcggctgcc gcacgtcgct cagcgcgcag cgcgcgatca
    10561 tgtacggacc gtgaaaaaga atctccacga tatcgagctg gaaggccacc atttgattcg
    10621 ctcggcggtg ccgctggaga tgaacaaggt gggaatgtga gcagagtttt accctatccc
    10681 caaggcgagg ggaccgcccc tgcacttaac aggatctggt gttaaatctg ttcatctata
    10741 ccctctcccg aatgaccgcc cctgcgctaa acaggatctg gtattaaatt tgcttttcat
    10801 acaatcggta acgcttgtac ggctccgccc caatgcgttc cagcatgtta ttcatgcctg
    10861 tattggtttc gaggatccat gacatctcca gcgcatcgat cttccggcgg gcaaacggat
    10921 cgcgtaaggc ttcaatcaat aacagcgcaa tcaccgggcc gatgcggctg aactgatact
    10981 cgtcgcgcac gcccatcagc ggtactcgcg cagttcgcac accgctgact ttcaaacgcc
    11041 acagcaattt tgcccagccg aagggaaaga gcgatccgtt cagatcggca atcgcctcgt
    11101 tgatgttagg caagccacaa taaacgcgca gggtgcagaa tcaatctcag cgatgtagat
    11161 catatcgtcc ggcaccagat atttaagttg gtcgcccatg gtcgcgaatt catgttcggt
    11221 aaacggcaca aatccccagt tgtgctgcca gccagagttg aaaatctcac gcaggatctg
    11281 catctcttcg gcaaaccgct gacgattgat gcagcgaatg gtcacctttt tgcgcacctg
    11341 atccatcagt ttttttagcg ccggagagaa agtgagatcg gttcgctgca tccaccacgc
    11401 cagtaaatca atgcctttgt gataacccag ttgttcaata tgcgcggcat accacggttt
    11461 gccgtgtggc atcatcgcac agggtggtgt gtcaaaacct tcaatcagta atccgctttc
    11521 ctgattgata ttcaggctga aaggcccgct gatcttactt gcaccttgtg acttcaacca
    11581 cgcttccgct gcgccaaaca acgcggcaaa atcctgcgga tcatcaatgg cgtcaatcat
    11641 gccgaaatga ccggtatctt caccgtaacg ctcacggtgc aaggtatcta tttgcgcggt
    11701 aatacgccca actatctgcc ccgctttttt tgccacccac gcctgccaga tgatatggtc
    11761 cgtccccgga tttttcgcag acaaatgctc gttgcgttca atgaataaag ggggtatcca
    11821 gtttggatcg tcaggataaa gtgatgacgg aaaagcgata aatgccttaa ggtcattttt
    11881 attaattact ttttcaattt taatcatcag cagatcgcat tccatatctc tgtggataac
    11941 aaataattaa tggtgaaaaa caggttgcaa cagcaacgag cggcatctta ataatcaact
    12001 caatgaatac aaacgacgaa taaatagtat taattaatga ttatgataag gtcagaggct
    12061 acacagaaac gcctcaatgc cttttgtcaa tttaaggcga ccggacaagg cttcttctta
    12121 atatattcat aaattaaata tcattcataa taaaaacaaa aaacttaaac tcattattta
    12181 aatcaatgtt cattttcaca ccgatgaaaa tactatctca tttatctttt atgcctatat
    12241 aatccaccgc attattgtca ttttattttt gatatccaat tttttcgtgg tgtatatgtc
    12301 taataaaatc tttacgcatt ccctacctat gcgctatgcc gattttccaa cgctggttga
    12361 tgctttggac tacgccgctc tgagtagagc cggaatgaat ttttatgaca gacgttgcca
    12421 acttgaagat caactggaat atcagacatt aaaaacgcgt gccgaagctg gtgcgaagcg
    12481 gttgttatcg ctgaacctga aaaaaggcga acgcgtggcg ctgattgccg aaacgagtag
    12541 cgggtttgta gaggcttttt ttgcctgtca gtatgctggc ttagtcgccg tcccgttggc
    12601 gattccaatg ggcgtcggtc agcgggattc ctggagcgcc aaactccagg gtttactggc
    12661 aagttgccag cccgcagcca ttatcactgg tgatgagtgg ttgccactgg tcaatgccgc
    12721 gacgcataac aaccccgaat tacatgtttt aagccacgct tggtttaagg cattaccgga
    12781 agccgatgtt gcgctccagc gtccagttcc gaacgatatc gcctacctcc agtacacctc
    12841 cggcagcacc cgttttcccc gtggcgtcat tatcacccat cgcgaagtga tggctaatct
    12901 acgtgctata agccacgatg gcattaaatt acgccctagc gaccgctgcg tctcctggca
    12961 gcctttctac catgatatgg gactggtcgg ctttctcctg ccccccgtcg ccacgcaact
    13021 ttcagtagat tatttgcgca ctcaggattt tgccatgcgt cctttgcaat ggcttaaatt
    13081 gatcagtaaa aatcgcggca ccgtttccgt tgcgccgccg tttggctatg aattgtgcca
    13141 acgccgcgtg aatgaaaaag atctcgctga actggatctt tcctgctggc gcgtcgctgg
    13201 tattggtgct gagccgatct ccgcagaaca actccatcaa ttcgctgaat gtttccgtca
    13261 ggttaacttt gacaataaaa ctttcatgcc gtgctacgga ctggcagaaa atgcgctggc
    13321 tgtcagcttc tctgatgaag cctccggggt tgtggttaac gaagtggatc gcgacatcct
    13381 cgaatatcag ggtaaagccg tcgcgccggg tgcagagaca cgcgccgtat cgactttcgt
    13441 caactgcggc aaagcgttgc cggaacatgg tattgaaatc cgcaatgaag caggtatgcc
    13501 ggtcgcggaa cgtgtggtag gccatatttg catctccggt cccagtctga tgagcggtta
    13561 ctttggcgac caggtttcgc aagacgagat tgccgcgacg ggctggttag acaccggcga
    13621 cctcggttat ctgctggacg gttatctgta tgtcaccgga cgcattaaag atctgattat
    13681 tattcgtggc cgtaatatct ggccgcagga tattgaatat atagcggaac aggaaccgga
    13741 aattcattct ggcgatgcga ttgcttttgt taccgcccag gaaaaaatca ttttgcagat
    13801 ccagtgtcgg atcagcgacg aagaacgtcg cgggcagctt atccacgcgc tggcggcacg
    13861 gatccaaagc gaatttggcg tgaccgcggc tatcgatctg ttgccgcccc acagtattcc
    13921 ccgaacgtcc tccggcaagc ctgcccgtgc ggaagcgaaa aaacgttatc agaaggctta
    13981 tgctgccagt cttaatgtgc aggaatccct ggcataaatc aaactgtcgc ggtgacgggc
    14041 gctaccgggt ttatcggtaa atatattatc gataacctgc tcgcccgcgg ctttcacgtt
    14101 cgcgcattga cgcgtactgc ccgcgctcac gtcaatgata atctcacctg ggtgcgcggt
    14161 tcgctggaag atactcattc acttagcgag ctggttgccg gagccagcgt agtggtccat
    14221 tgcgccgggc aagtgcgcgg gcacaaagaa gagattttca cccgctgtaa cgttgacggc
    14281 agcctgcgcc tgatgcaagc agcaaaagag agcggctttt gccaacgttt tctgtttatc
    14341 tcttcgctgg cggcgcgcca tcccgagctc tcctggtatg caaaatccaa atacgtcgcc
    14401 gagcaacgac tggcagcaat ggcggacgaa attacgctgg gcgtttttcg cccgacagcc
    14461 gtgtatggtc ccggcgataa agagttaaaa ccgctgtttg actggatgct acgcggcctg
    14521 ctgccacgac ttggtgcgcc agatacacaa ctctcttttc ttcacgtcac cgattttgcg
    14581 caagcagtgg gtcagtggtt aagcgccgaa actgtacaga cgcaaaccta tgaattatgc
    14641 gatggcgtcg ccggtggcta tgactggcaa cgcgtacgac agcttgccgc caaagcccgt
    14701 tgtggttccg tgcgaatggt tgacattcct ctgccggtac tcacaggcct tgcggatatc
    14761 agtaccgcgt tgagtcgcct ggcaggtaaa gaacctatgc tgacccgctc gaaaattcgt
    14821 gaattaaccc acgccgactg gtcggcaagt aataaccgta tttctgaaga tatcaattgg
    14881 tttcccggga ttagcctgga acacgcatta cgcaacgggc tattttgatt gtcttgtttt
    14941 aaaggtgtgt taacgtttct atggtaaatc gtgaaatagt aatggattac atcctttcct
    15001 gtttacagga tttggtcgaa aacggtgtgg aaattaagcc cgacagcgat ctcgtcaacg
    15061 atcttggcct ggagtcgatc aaagtgatgg atctcttaat gatgctggaa gacagattcg
    15121 atatttccat tcctattaat attctgctgg atgtcaaaac ccctgcgcaa ttaatggaaa
    15181 ctctacttcc atggctggag aataaataat ggggctatac gataaatatg cgcgccttgc
    15241 tggcgagcgt ctgcaattta gtgacaacgg gttaacaccg tttggcacct gtattgatga
    15301 agtgtattcc gccaccgaag gccggatcgg caataaaaaa gtgattctgg ccgggaccaa
    15361 taattatctc ggtttaacct ttaatcatga tgccatcagc gaaggtcagg ccgcgttagc
    15421 ggcccaaggc accggcacca ccggttctcg tatggcaaac ggcagttatg cgccgcacct
    15481 ggcgttagag aaagagattg ccgagttttt caatcgccct accgctatcg tttttcaacc
    15541 ggttataccg ctaatttagg cgtcatcagc gcacttgccg accataacgc cgtggtgtta
    15601 ctggatgccg acagccacgc cagtatttat gacgcctgtt ctctcggtgg cgcggaaatt
    15661 attcgtttcc gccataacga tgctaaagat ctggaacggc gcatggttcg cctcggtgaa
    15721 cgggcgaaag aggcgattat catcgttgag ggcatctaca gcatgctcgg tgacgttgcg
    15781 ccgctggcgg aaattgttga tatcaaacgt cgtctcggtg gttatttgat tgtcgatgaa
    15841 gcgcattcgt ttggcgtgtt aggcgcaacg gggcgcggac tggctgaagc tgtcggcgtt
    15901 gaagatgatg tggatattat cgttggtact ttcagtaaaa gtctggcctc gattggcggt
    15961 tttgctgtcg tttcggaagc gatggaagtg ctgcgctacg gcagccgccc gtatattttt
    16021 accgcctctc cttctccgtc ctgcattgcg accgtgcgct cgtcgttaag aactattgcc
    16081 acacaaccag agctgcggca aaagctgatg gataacgcca accatctgta tgacggtctg
    16141 caaaaactgg gttatgagtt aagttcacat attagcccgg tagtgccagt aataatcggt
    16201 tcgaaagagg aaggtttacg catctggcgc gagcttatct cgccgggcgt gtatgtgaat
    16261 cttatcctgc cgcctgccgc gcctgcaggc atcacgctgc tgcgctgtag cgtaaatgct
    16321 gcccacagtc gtgagcaaat cgatgccatc attcaggcat ttgctactct gaagcagtaa
    16381 tgtgagcccg ccgtggtgtt tgcgccacgg cgggtttaat gttgtctgtc ataccaatac
    16441 accaggctca acgccaccaa cacaaacgct accggtaaac cgagcgcggt aacaggtgcg
    16501 cttaacgcaa atagcaatcc cagattaacc atgatttgat agctgatcca ggtgagtaag
    16561 cccacgatga cgcctacagc gagacggcta cccatcccag gcgaacgcgg ggcactaaag
    16621 gtaaacggca cagccagcaa aatcatcgcc agggtcagga tgggttgccc cagtttttcc
    16681 cacagtgcaa ggtggtattc gctgctgggt tgcccggttt cctgcaaata atggatgtaa
    16741 tgattaagct gacggacaga aaaagtattc cccggcattg ataactcttc aagattcata
    16801 ctggtgaagg ccgattgcca ggcaagatta tctaatgttt ccagcgtttc tttgccattc
    16861 agccattttt tatgattcac accatgcagg acccacgttt tgtcgtcttc aatggtggca
    16921 ctgcgtgcat aaatgtagga ttcaagcgag agatcgtcgc gataatgaaa tatctccacg
    16981 cccaccggct ggccctgctc gttcagcgat ttcaccgtca caaattcatt accacgcctg
    17041 gcccacagca tattgccggt aatgtcatcg tcctccccca acgcggtggc ggtcgatttg
    17101 atttgcaacg cctgttgctg caatggcgac gccacccact catctatcgc gcctaacgaa
    17161 acagtccaca atatcccggc aaccagcgcc accagtgcaa tacggaagat agaaaacccc
    17221 gtgctgcgaa tggcggtaag ctcactgttt ttcgataact gccccagtcc aacaatacca
    17281 cccaataacg cgataaacgg cgaaagttcg accagtgtgc gcggtaaggt cattagcacc
    17341 accagcaccg cctgagtcca gcgataaccg cccgggctga caccatccag ttcgttaatc
    17401 aggttgaagg tggtaaaaag cggcagcaat agccctgcag cggcggcaaa accgagaaag
    17461 agatgacgga ttaaatagcg actgaaaaca ttcatcggga aaatttctgc agcaaagaga
    17521 agtcacgtgc gaccagtatc agcaggccca ttagcatgag tccgggcact aaccacaaac
    17581 caggaatgag gggaatcgcg ccgttagcca ccagtgtacg gcagacgtcg ccgccataaa
    17641 aaatggcaat aaacagcaac gtcagtggca ataacgttga aaatcgccct tgccgcggtt
    17701 ttacccgact taatgaaatg gccagcagcg ccatcaacaa tgcggtcagg ccacggcttt
    17761 gtcgccattg cagttcggca tgatcggcag gaaaggcgga gcgcgccagc tccgtgaccg
    17821 atttcgcttt gcgtttgacg ttcgggcttt gatccagcgg attcagatgt aattgcagat
    17881 tacggtagat ctgttcgttg tcgtcacgcc cctgatgatc gagaagatag gcagtgccgt
    17941 tgtgcaacat aacggtcggt ttctcgggtg atgggtcaac cacatcaacc gaaccggcgc
    18001 ggaaaatacg ggttcgattg gcagtagaag tataaatcag cgcgtcagtc aggcgattat
    18061 tatcctgatc aaccgtctgc gaaaggatca ttcgtccgtt atcgttagtg ttaaatttct
    18121 ttgcccgcaa ctggcgaaca tccagctccg actgcgactg ctgctccagt tgataaatct
    18181 gcgcataagc ccacggacga ccatacatcg acagaagggt gacaaaaata cttagcggta
    18241 tcgccagata aagaacggct ttgtacaagc gtcccggact gccccccgcc gcagagatag
    18301 cggtaatttc cgagtcggta tacatctgcc ctagcgtcac gccaactgac acatacagac
    18361 caacaggtaa caacatctcc agtgcaatca gtactttgta gaaaacgata tccagcacaa
    18421 catcaagagc taatgtccca tttgccgctt cggtcagata acgctgtgcg gagtagctgg
    18481 cgaaaataaa gatcagaaaa ccgacgataa tcagcaccag acggcgcgta ccacgcatga
    18541 tgtagtgttc aaccagcttc atctcactaa cgccctcaat cctttgagtt ttttattcat
    18601 tgatcagttt ctgcgccaac gcaatgacat gatctacctc ttccggcgtc aggctgccgt
    18661 cctttgccca ctgaatttgc ccggctttat tcagcaccag aatggttgaa ctctgctccg
    18721 gcaagcgcca ggcaacgcgt ccctgtccat tgccgtcaat aacgaattgc gcccacggat
    18781 agcggcgttt atttttttcg attttgccaa caacaaaata gccagtacca aagatggcat
    18841 catcggtgtt cacaatggtg gtgggctgaa aacgatcctg cggaaagttt gccgcttcaa
    18901 ccgctttaat taacagtgag ttctttttct tcgccgactt acgcccggcg atatactgaa
    18961 taatgcggac ttttcccgct aattgtgtgc tgttccactt ttggtagtga atttcgtcgt
    19021 cgctattcag aagcagctcg ccgccttcct ggatataaac aggggtaacc gttttgccag
    19081 tgacgaaatt gtgtgcctgc acacccgggg ctaagagcat gataatcaac gcaataatca
    19141 aacgagatga catcattgct ccggctatag cggaaaacgc gaaatcagca gctctaagcg
    19201 ctgacaaata tcgctatcca gcgccaacgg tgcataatac gactggcggt tttccatatc
    19261 atccgccacc atgtcgtcgt gtttatctcc catcaggcca ttaatggcgg cgaggaaacg
    19321 ggaaatctgt aacgcctctt tattttcagg atcaagcgat cttaaggtgt cgtaataaga
    19381 aacctgctgg ctgatgattt ttcgcacatc atgaaattcc ctgccggtta gtcgcggttt
    19441 ggcaataatc tctttcaggg tatgcatttg cttttgccga taggcaataa aactgcttac
    19501 gctttctaac tggctatggc gtaatgaata gttcaccaac gaccagaccg gagagcttaa
    19561 ataaatccgt aacaggttgc cgtaatacga cacaatattt ttcttaccgt tacgaaagcc
    19621 gtcctgaaaa cgccccagaa aaacggtcgt tttgccgaat aaaaatccgg cctgatgctt
    19681 ctttaaatac agccgttgcg caaaacgcag gtgcttgtag cgcgctcgca tgtatttaaa
    19741 ttgtttgcgc tcatcagcag tcagctcatc gcctttggct tgtttgcgaa ctaaacgcag
    19801 taattcctga cgatcaacgc cattgagcca gaattgcagg cataacgcgt aattatcatg
    19861 aatgatttcg gtggtacaac ttagtgcaat agtcggcggc aaactaacct gtgcatcaac
    19921 gacatcatct tcttcaatcg caacaaatag acgctcgctc gcctcaagcg ggaaatattt
    19981 tctcatcatc aggatttctc tccggctgcg ttataaagca aggaatggcg ctgaaaaaat
    20041 gcctttaagg ttggaattaa cgagagcgcc acgtccagac attgcccacc gaagtaagca
    20101 aacgctgctc ccagcacacc aaacagatga cagaacatga acagcaatac gatgtagccg
    20161 attgaggcga tggtttgcgc cacgagaaaa gcgcgctgtt ttcccgccat caatagcaat
    20221 gactcctgcg ggaagcccag catcgaaatc acaattgcgc ccagcatcac ctgaatcagg
    20281 tcatacgctt cgagatactt aacgccaaac accagcgaaa tgagcggctt gccgacgatc
    20341 aacaccgcga gcgccaccag aataccgatt ccgcccgcca gtaaaccgga cttcacacct
    20401 aacaaccacg gtctggtggt gcgcggatct aaacgcatca cctctgggta aaaactttta
    20461 cccagcaaac ccgctggcgt tccggcggcg tcgaagagtg tcatggcgat tttaaataac
    20521 ccggcggcag cgggtcctaa cacgatcccc accaacactg tgctgcacga gttacgcgcc
    20581 gaccagatgg agtgggcaat gttggttgac cagacaaaac tccacgcgcc tttaatgtgt
    20641 cgggcagact caaacagatt caatttgaag gcgttatgga tatttctacg gcgtaattcg
    20701 cgcgcggcaa accaccagta catggtgccg ccaaccagat tcgacacgta ccaggcaata
    20761 acaaaacccg caaaaccaaa gtcaaaatac caggctgcga cgctccccgc tgcgcgcaga
    20821 aaaggtttcg tcgcctgctg tacagcaatc aaatcaaagc gatctaccgc acgcagaatg
    20881 ccggtcggcg tggaggaagc cattgaagga atgagcgtgc aatacagcgc cgccagccaa
    20941 aagctttggt catccagacc taatgaatgg gaaaggaatg gcagtaaggc aatgccgccg
    21001 acaatcgcca ccgcgccgct gacgatatcc agcgagaagg aaaatgagac gacattgcga
    21061 aattgctgcg gattattgtt ggtcaaggct ggcgttccgt actgaaccac cagctgccat
    21121 gtctgaaact taataaaatc gctgatcgac ttggcgtacg attgcacaat caccagtaca
    21181 ccaaacatgg cgggcgtcat ccctttaccg gcacacgaga gcgccaacag ccccagcaag
    21241 gcgctctcga cattactgga gcctaaccag gcgctattgc gaataatggt gcgaaacgcg
    21301 ccatctgcaa accaatgttt gatgttaaaa cccgccaatt cagcctgacc ttatcgttga
    21361 tagtaaaaaa tatcccgcca gccttaagtt aaacttcggc ggtcagaaac gatggcaacc
    21421 agagaaaccg ccttctgtac ctgttccagc acttcgctgt agggcgctct ggaatcaatc
    21481 tcaagaattt ttgtgccgtt atagccaatc gtcgacatga cgccgatttt gtcctgcagc
    21541 tcggcatact catggtcagg cttgcgggag atggcagtat caatatcaat ccccaaacga
    21601 atgattaatt caggacggta ttgcgccatt ttttggtata aactccgttc gcgctgcgcc
    21661 agaaacatgc tgattttccc ggtcgcacgt tcgacgccga tccccggtcc gtcataataa
    21721 aagcccgaaa tttcagcctg cgggaagcga tcgctgacca ccagaacgcc actttgcgcc
    21781 agtcgctgaa ccttgcgtag attcgccatt cttcgcagcg agaagcagta cataatcacc
    21841 gccgcccata gcgccggaga tttggttttc atgctttggg ttttcgagga tttggccgcc
    21901 aatcgccgtt ccagccagac gccaaccaac ggcaatcgtt tgattttgtc gccgtcttcg
    21961 ccggagagca gccccagata gcggcgctcg gtttgccagt gttgttgcag cgatttcacc
    22021 aggtcggtgg tcagcgtgga tttaccggtg ccatcacacc cgaccaccgc aatcagcccc
    22081 ggaatgtagt tgggctgcgg cgcggttgta ctattaacag tttgtgtttg tagtgcatcc
    22141 attcgttaaa tcatccccta tccattttgg tgatcaggaa agcgcggcgt gaattgcgcg
    22201 tagtgacgct tgcataattt catcggctgg ttgccgacca tccagttcaa ggatcttggc
    22261 gccattgaat gtcagttgcg gcgtaacggc gattttttcc tgtaacgctg ccagttggtg
    22321 gtcaggttta cgcgcaaacg cggtttgttc atcaatgcca agacgaatca acaatacggg
    22381 taaataagac gccatccatt ggtacagctt caactcgcgc tgccttaaca tttttatcca
    22441 accgttaccg cccgtggttt ttgccaattg cgggccatca aagcgaaaac ccggcacttc
    22501 aacctgcggg tagcggtcgg tgatgagcag atagccttgc tggcttttac acaacatttt
    22561 gcgaaacttg tacgcccgcc agcaggaaag cagatagata accagcgcag taatattgcc
    22621 aggcggagtt aagggctttt cgtgtacatg tgccgcttta cttcgcagat aacacccaca
    22681 aggtgcgcca ataacaggga gctgtgaaat ccattcgcca attcgcccgg acgattgccc
    22741 gagataaatg tgttctgttg gcattcttgc tgccagttca tttaccaggc ttgccgtgag
    22801 ggtcgattta cctgaaccat cacatcctac aatggcaata acacgcactg gcgtggaatt
    22861 aattattgac atattacgtt gattcacgaa aaacccggca gtaaataatg tattgaatat
    22921 tacgtggtcc gtgctcagaa tatccgttca accttattgc gacaatggct aattgtgaca
    22981 ttgacctgaa ttacgctttc ataaaaacat attaaccaaa taaatatttt taatggatat
    23041 ttaaattaaa ggatatattc atgcagtcaa taacacctcc attaattgcc gttattggta
    23101 gcgatgcttc aggcaagtca acggtgtgtg aacatcttat taccgttgtc gaaaaatatg
    23161 gtgctgccga aagagttcat ttaggaaaac aggccggaaa tgtcggtcgt gcagtgacaa
    23221 aattaccttt gatgggaaaa tccttacata aaacaattga acgaaatcag gtgaaaacag
    23281 caaaaaaatt gcctggacca gttccggcgc tggtaattac agcgtttgtc gcccgtcgct
    23341 tactgcgctt tcgtcatatg cttgcctgtc gtcgtcgcgg gttaattgtt ctaaccgacc
    23401 gttatcctca ggaccaaatt cctggcgctt acgatggtac ggtgttccca cctaacgttg
    23461 aaggtggtcg ttttgtctca tggctggcaa gccaggaacg taaagcgttt cactggatgg
    23521 cgagccataa gcctgatctg gtcatcaaac tcaatgttga ccttgaagtt gcctgtgcac
    23581 gtaaacccga ccataaacgg gaatcgctgg cgaggaagat tgccataacg ccacagttaa
    23641 cctttggtgg tgcacaactg gttgatatcg atgccaatca gccactggaa caggtgttgg
    23701 ttgatgcaga aaaagcgatt acggatttta tgaccgcgcg tggttatcac tagtcaaaaa
    23761 tggaaatgcc cgatcgccag gaccgggcat tttcaggaag gttaaatcaa ctgcaatgct
    23821 atccagtaca gcccaccaga aagaaaaatt gccgccggta aagtaaacac ccacgccatc
    23881 aggatgctgg ttaccgtttt acgctgtaac ccaccgccgt ccaccaccat cgtccctgct
    23941 accgcagacg agaggacgtg tgttgtggag acaggcatcc caatataact ggcaaggccg
    24001 atagacactg ccgccgtcat ttgtgccgcc atgccttgcg catacgtcat gccgcgctta
    24061 ccaatcttct caccgatggt catcgctaca cgacgccagc caatcatggt gccaatgccg
    24121 agcgccagtg ctaccgccat gatgatccac accggagcgt actcaatggt gcttaacata
    24181 tcgctgcgca gttttttcag caggttctgg tcttctttac tgacgcctgg cagtttcgcc
    24241 agcttcgcgg aggtatcgga gatgcacagc ataatgcggc gcagctggct gcgctgactc
    24301 acgcttaacg gctcgtaact ttccatattg cctggcagca tcgttttaac gcgcgcaatc
    24361 gcatcaaagg tatttgccgg atgacagtga aactctgtta cttgcgtgcc atcagtcgat
    24421 gctgcaggca atggaggttc catcgcaatc aacttctgcg gcagttcagg atgctgttgc
    24481 aggtagtgtt cgaagttggt aacggcatcg cgggtacggg taatttcata gccggacgca
    24541 ttcatattga cgacgaagcc agcaggggca atccccacca gtaccagcat taccaggccg
    24601 atccctttct gcccgtcgtt cgcgccgtgc gaaaacgcca cgcccgcagc ggaaacaatc
    24661 agcgcaatac gcgtccagaa tggcggttta cgtttgccct ttttcttttt gcgatcttcc
    24721 ggaatgcggt gaatacggtc acgctttttc gtcccactcc agaaacggcg cagcaggaaa
    24781 atcagaccac ccgcaatgac caggccgacg ataggggaaa caatcagcga ggagaaaatt
    24841 ttggttactt cacgcaggtt taacgcatcc atcaccgatg agccggttaa cagcgcgttg
    24901 gttaaaccga tgccgataat cgcaccaatc aaggtgtgcg aactggaggc cggtaaacca
    24961 aagaaccacg ttcccaggtt ccagataatc gccgccagca gcatggaaaa gaccatcgcc
    25021 aaaccgtgag ttgaccccat attcagcaac aaatcgattg gtaacatatg gacaatggca
    25081 taggcaacgc taagtccgcc caataacacg ccaaaaaagt taaaaaatgc cgccatcacc
    25141 acagcaagtt gtggctgcat ggcacgagta taaataacgg ttgccaccgc attcgccgtg
    25201 tcatgaaaac cattgattgc ttcgtagaac aacacaaatg ccagagcaag taataacaaa
    25261 agccccgtat atatatcaag gccaacaaat aaatttagca tatattagat tacgccattt
    25321 tgaatttacg aacggacgca ttatcagtga ctttaacggc atgggcaaag tgaaatatca
    25381 ttttttaacg ataaaaagag tgttatttct tgtgtatcag acaataagca ttcattcaaa
    25441 acctttagaa ataatcaaaa cacaggtatt ccactggtta atatattcac atatgaaatg
    25501 aataaatatt ggaatatata aatattgaat attttgatta atccccgtac taattattct
    25561 tcataatcag tacggggttg ctacaacatg tattactttt tcaccacaat taacggttca
    25621 atatcactct cttttttgat gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag
    25681 ttgccgccaa cggtgaaggt acatacctga atgtatttac cgtccacttt cggcaaacac
    25741 cacagttgct gatagatgtt tttctgctcg gcaaatttac cgctggtttt gtccagcacc
    25801 tcttcatgat ggctgaccag gtcgatattg ctaccacagc gaccggcgat cggtttcact
    25861 gcgtaacctg ttttcaccaa ttcatcatta acagtgaaat cggtatccag caggtaacga
    25921 tggtgcggga acagcgacca gaggatcggc agaatcgctt tgttgccggg gatcaccgtc
    25981 cacagcggct caaagaccag cacttccggg cgcagcaata cgtcgataag acgcacttcg
    26041 ttttgcggat gaccggtacg gattggcacc gcagcaaact cacggtcgct aacttcacga
    26101 atctgatcaa acgcggtttc ccacgcccag gttttccaca cgcagttaac cagtcgccct
    26161 tccccatcaa tcagttgccc ggcagcatcc cagcccagtt catccagccc gcgcaagata
    26221 cgcgtttcaa agcccgcctg gtgcagcgcc tgctccataa actgcgcgtg atagttttcc
    26281 tcgatatctt tgtcctgcat gatatggaca aacggacgtg cacgactgtg tttccaggca
    26341 ccagccagtt cgttaatcag cccttccgcc ggattgaagc cgttgccttt atagccctgc
    26401 tccgcccaac gttcgaggat caagcccgct tcggtatgac aggaggcgga gtcggcgttg
    26461 tattcgtaaa ccttcaggcc acgctcatcc atgcagaaat ccatacgacc agtgatcata
    26521 tggtgacggc gacgctgcca ggagagacgc aaacgtggcc agaggatttt cggaatgtcg
    26581 aacagcgcca gcaggttgtc atctttcagc accttgtcgg ttgcgtgaag atacatcaga
    26641 tgcagctcgt tggtggcttt aattagctcc tgctcggcac tctcggtaat ggtgtagtaa
    26701 tgatacggat cctgattgat cacctgaccg ttggcctgca cataggcgtt ttgtagcgga
    26761 tctttttcat ccagccattt accgtcaaac tggcctttgt tttccagacg cgctccactg
    26821 attttcagca gctcgcctgc aatttccggc tgcggtaagc tgtattcagt atcttccgtc
    26881 tggatcatcc agcccagaat ggtggtgtca tcaaaagtgt ctttaagggt atagcagccg
    26941 ttttcgacca ccatctccag ctcgcgcgtc cactgttgcc cttgcggcaa cggggaatga
    27001 atcacgttct gttccgcaat acggactttg ttgccatgca attgggtaat gatggcgaca
    27061 tggccagtgt ctttaaattc accgccttta tcccagataa gaagcgcacc cgcgaccggc
    27121 gcacgcggcg agccgttagg aaatgcctgc aatggcagga tgttgtcatt aaccacttct
    27181 cgcaggaagc gcagcgagaa aatctcccac gccataccca cgtcagtaaa gaccacaccg
    27241 taattcagaa agagaaaacg gcgagcaaat tcaacgcatt gccacttgtg gcccatatat
    27301 tcgtcgtcga tatagctacg gaatacggcg tcatcttcgt attcctgcgg atcgagagaa
    27361 ctgtaatctg aagagtagat tgctacccca cctggggcgt agcccaataa tgtcccgaac
    27421 ggggcatcct ggctggtcgt tcctttgctc atcactttac ctttaacaat acaacctaag
    27481 cagttggcgt aattgttgtg ctctgattac ctgctcagcg acattaaccg gacagaggtc
    27541 agactaatca tacacctcat cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg
    27601 tacagcaagt gacctgctac actgcttcaa cactaccact cagaaggcaa ctcactatga
    27661 cagacaatac ttatcagccc gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt
    27721 tcgccaatat caatcgcccg ttttctggtc cgacgcatga aaaaacgctg cccgttggca
    27781 aacatccatt gcaactttat tcgctgggaa cgccgaacgg tcagaaagta acgattatgc
    27841 ttgaggagct actggcgctg ggcgttactg gtgcagagta cgacgcctgg ctgattcgta
    27901 ttggcgatgg cgatcaattc tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc
    27961 cggcgctgcg cgatcatacg cataatccgc cgatccgcgt gtttgaatct ggttcgatcc
    28021 tgctttatct ggcggagaaa tttggctact tcctgccgca ggatctggca aaacggactg
    28081 aaacgttgaa ctggctgttc tggttacagg gtgccgcgcc gttcctcggc ggtggttttg
    28141 gtcactttta ccattacgcg ccggtgaaaa ttgagtacgc catcaaccgc tttaccatgg
    28201 aagccaagcg cctgctcgac gtgctggata agcaactggc gcagcataag tttgttgcgg
    28261 gcgatgagta caccattgcg gatatggcga tttggccgtg gtttggcaac gtggtgctcg
    28321 gcggcgtgta tgatgccgca gagtttctgg atgcgggaag ttataaacat gtgcaacgct
    28381 gggcgaaaga agtgggcgaa cgtccggcag tgaaacgtgg gcgtattgtt aaccgcacca
    28441 acgggccgct gaacgagcag ttgcatgagc gccatgacgc cagtgatttt gagacaaata
    28501 cagaagataa gcgtcagggg taagggttgg tgttcgtcgc agcaagccat ccaggccgga
    28561 taaggcgttc acgccgcatc cggcaatcgt gcacaatgcc tgatgcgacg ctgccgcgtc
    28621 ttatcaggcc tacaaaagct aatcatcacg cgctggtaat gtcgtaatcc atttggcgca
    28681 gtgcgtctaa tgtggctttg gcttcatctt cgtcgatgat gctcatggca aatccgacgt
    28741 gtaccagcac ccactggccc agcagatcgg cagggttacc ttcgcaaatc agggcgatat
    28801 tcacgtcgcg cttgatacca catacttcaa cctgcgcaag ctggtgaata tcttcaccga
    28861 cagccagcac ctggcctgga acgccaatac acattgttaa ctccggttat ttcgattgcc
    28921 ggacaaacgc gaaaaagaac ctttagcggg tgttccctct caccctaacc ctctccccgg
    28981 aggggcgagg ggaccgtccg tgcgcttttt aggcgttgtc ctcagtctta ttcaacttca
    29041 atacttttga cgatcagcga atcgccggta tcgacacgca accgctcgcc gtgacagagc
    29101 ggacactgcg catcgtgctg atgaatctcc accacctggc tgcaatccca gcaccaggcc
    29161 tgggcgggtt tatagacgat atgtaaatcg cacccttgcg ccaccgttcc gtggcagaca
    29221 atttcaaaac taaaacggac ggcgctctcc tcaacgcagg agagcgcgcc aatttccagc
    29281 cacacggcgg tgacgcgctt aacatcgtgc tgctccgcct gccgttggat aatttcaacg
    29341 gcgctctggc aaagcgacaa ctcatgcatt ttcgcccctc ctgcggccaa acagcagggc
    29401 gcgacgccct gcgtgtggta catccggatt ggtgactggc aacgaaagga tcattcgtgc
    29461 gcagtcatcc gtcaggcgtt ggccttcttc aatcgacatg ctgtgcgaaa gcggcgacat
    29521 cagcgaacag gagaggtatt gcgaaacacc gtccagttcg ccaacggtaa aggtcatggt
    29581 gccatacggc agttgcagac caattttttc actcactttg cgcagcggcc agagttgatc
    29641 cgggccgggg aagataactg cactcagcat ccacggggtg atcacacacc ccgtccactg
    29701 accttcgaac agcgtaaaat cagaaacata caccggcatt gaaggatgca gaaaagagag
    29761 atcgtgcatc gaacgccggg caatttcttc aaacgctgcc tgtacttgcg ccttcgggga
    29821 ggtctggaaa cctgctatct cttcagtcat gaatcgcctc ccgtgggatg gcttccacgc
    29881 ctgattcgcg cagcgcagcc agaacctgct caagcgcagg ttcaatcatt gcttcaaccg
    29941 tcggcgttaa gccgatgtgt ggctccagcg attccgggat cacgccgacc agggtcagct
    30001 ttttcggaaa ctcaccggtg aagcgcaggg ccgacaatac gtcggccagg ccaagctgat
    30061 gcggagagat tttgttggta aacaacgccg gaacttcttc atcccgcagg atcatcatcg
    30121 ttcccggagt gttctttttc gacacaatgg catccgcaat aatcagatga tcgcgatttg
    30181 ccatgtcgcc aagcagctcc attcccgccg tgccgccatc gaggatctca acataatccg
    30241 gcagaatgta tcgttgctct aacgcttcga caatccgcac gccgatggct tcatcggtca
    30301 gcaaaatatt gccgacccct aagactaaaa tacgcattac agaaccttca ctgaaaccac
    30361 ttcgttgccg tcggcatcca ctacgtgtac cgcacaggcc atgcacgggt cgaaggagtg
    30421 aatggtacgc accacttcca gcggtttatt cggatcggca accggtgtac ccaccagcga
    30481 ctgctcgtaa ggaccgacgt catcattgaa gttacgcgga ccagagttcc aggttgatgg
    30541 aacaaccgcc tggtagttgc tgatgatacc gtctttaata accatccagt gagagagcat
    30601 accgcgcggc gcttcgagga agccaacacc tttgaactca cccgttgccg gaatgttcgg
    30661 cttcacaaag gtggtgtgat cgcctttgcc gatattggtg atcagtgcac tgtattggtt
    30721 ttgcaggata tcctgcaatt cacagcagtg aacggtacga ccaataatac ggcccagcgt
    30781 ggagtgcagc tgtgccactt ccagcgtgtt gccagtcagt ttctgataaa tcgcaacgat
    30841 ttcattcagt ttgttctggg tagattcgcg acctgccgcc agtttcacca gcatattagc
    30901 cagcggaccc acttctaccg ttttgccgta gaaagtcggt gatttcaccc aggaatattt
    30961 cccgtcgtca gaccaaccat cataagccgg aatggtggta ccttcccacg gcgcctgtgg
    31021 cgcttcgtct ttataccagg agtgcttcgc gctttcctga atgcctttga tcaggtattc
    31081 atcggaatga gaagtgatcg gacgatacga ggacagatcc gcattctcaa tgtagcctcc
    31141 cgggaacagg aagctgccgt ttttgctgtc ggtcgggaat tccggcacgc tcaggtagtt
    31201 caccgcacct ttaccgcgcg tcagccattc cgggtagaac gcggcgataa ccgcggtatc
    31261 aaccttataa acctgctcaa caaagtcgct cagtttgtcg atgaaagact tgatgtacat
    31321 caggcgctca aggttcaaca cgcccaaccc gtcgaggttg attgggttcg cgacaccacc
    31381 cacagccagg ttctgaatgt gcggcgtttt accgcccagc agcgccacga cgcggttagc
    31441 gtcacgctgg cactccagcg cctgcaggta gtgcgctacc gcaatcaggt tcacttccgg
    31501 cggcagtttc atcgccgggt gaccccagta gccattggcg aaaataccca actgaccgct
    31561 ggcaaccaga tctttgatct tgttctgaac tttagtgaac tcttccggac tgttcagatg
    31621 ccaggtcgaa acgcctttca gcatttcgga ggctttggtt gggtcagctt gcagtgcaga
    31681 agtgatgtcc acccagtcca gcgccgaaag ctgatagaaa tgaacgatat ggtcatgcgt
    31741 ggtgtgcgca gccagaatga tgttacggat gtattgcgcg ttaaccggaa cgtcgatatt
    31801 cagcgcactt tctgccgcac gaacggaaga cagcgcgtga gtggtagtac atacgccaca
    31861 gatacgttgc acaatcatcc atgcatcgcg cggatcgcgg tttttcacga tctcttccat
    31921 gccgcgccac atggtaccgg aagcccatgc tttcgaaacg acgccatttt cgatttcgca
    31981 atcgatgcgt aaatgcccct caatacgggt taccggatca atagtaattc tctggctcat
    32041 gctttgctcg cctcatgacg attatgatcg ttttgtttta aaggaggaag tatcggcagt
    32101 agacgaatga gtacgatgta agcgcaaatc tcaatagcca caaaaccaat agaaatcaac
    32161 agttcttccc aggtcgggaa gtaggcgtaa ccgccgcccg ggttgaatgc caccaacgaa
    32221 taggtcagac gccaggttgc acaacctaac agtgcgctca gtgctgacag gaacagcata
    32281 cgggaatcat tacgcagctt cgccacacgc agaacgacca gcgggaagag catcagcagg
    32341 acttcaatcc agaacatcac ggagtagaag tcaccggcaa acgctaacga cagcttgtcg
    32401 cgatagataa gctcgccaaa gcgcagcccg atgaaaatcg ccagcaacac actgatggtg
    32461 ttggtcagct tgacgaacag gctcttttca tccggaccgt tgccacgcag acccgcctgc
    32521 accagtgaac cttcaaagat gacaatcgag aagcccatga tgaacgccgt cagcagcgag
    32581 aacagcggca acatttcata gctctgccag agcggatgca ccttgtagcc cgccgagatc
    32641 atcagcgacc ccattgaaga ctggtgcatg gtcggcagca gcgcaccgag cgcgatgatg
    32701 aagaacatca ccttgttcag acgttgtagc gacaccttcc agcccagacg ttcaaacagt
    32761 gccggagcaa actccagagc catcacgccg atgtagatgg tcatacagac cgccgtctcg
    32821 aacagtaccg agttcacgtt gaagtgaccc ggaatgtaga agtacggcag gttccagtag
    32881 cgacccacgt cgatagtgat cgacaagcca cccagtgagt aaccaaacag actcgccaac
    32941 agcgccggac gcaccagcgg atggtattgc ccacggttaa agacgtatac cgcccacgcc
    33001 agcgcccagc cgccacaggc aaagccggtg ccaatcagca ggtcaaacgc gatccacacg
    33061 ccccacggga agccgccgtt caggtcagag acagagccca gaccgaacac cagacgcttc
    33121 acaatcagga gcatacagat gacgattaac ggtccaaaaa tcatgaccgg tttactgatg
    33181 attttgccgc ccagcggttg tggatcatga ctcatgatcg tctcctccgt cgtgatggtc
    33241 gtttttggtg ttgcgacgaa ccagcacggt taagcccgcc agcacagcca gtggtagcat
    33301 catgccttta tacagggtgt gttgaatatt ttcggaacgc gcaccggtag aaagatcgtc
    33361 cagtttcggc aggtcgagat tttcataagg cacacccgtc agtaccagaa cctgagtacc
    33421 gccgccctct ttctcgccgt acagatgcgg ataatatttc ggcaccgtat gcaggtaagt
    33481 gtcgccagat ttcagcgtct gacgcggata gtggtattcg ctgccaggct tcagcgccag
    33541 acgttttttc gcctccgcca tcagctcttc acgcgtaccg aaaatcaccg cgcccgccgg
    33601 gcacacttct acgcagccag gcagaccgcc tttatcgaga cgttccacac ctttctggtt
    33661 gcacagctcg cacttatgca gcgcaccaaa cgggttgttg tagtcgtact tcggcacgtt
    33721 gtacggacag gcgaccatgc agtaacggca accggtgcac acgtctttgt cgtaatggac
    33781 aatgccggtt ttcggatctt ttttcagtgc agagaccggg cacacagaga cacagttcgg
    33841 atcgacgcag tgcatacact gtttcttaat gtacgcgtag ccgttctcct cctggtcttt
    33901 gttgacccct gtgccgctgg tccacacctg aatgatgtta ttggtatacg gcgacagttt
    33961 gtcgttgttc gaccaggtct gttccccttg cgggttacgt tcagggaagt tgatatcctg
    34021 acacttggtg acgcaagcct ggcagcctac gcacaaggtc gagtcgtaca acatccccag
    34081 cgatcccgga attggcgggc ggttttcagc agccgcatga ctgacagacg gcagcgcgcc
    34141 cgtcagcaat gccccgcagg aggctgcttt aataaaatta cgtctgttca cggttattct
    34201 ccccgtgagt cagcgttatc tttcttttgc tgacgaccca gttcacgcac cgccatcacg
    34261 ctgacaccgg caaccagccc aaccacaccg ccgagcaaac caatagcgcc tgcagagacg
    34321 ttgccgccct ctttagcgtt aacatccggt ttctgtgaac gcggagtctg attttcgacg
    34381 ttggcaagct gatggatgcc tttatggaag ccgatacctt tttcgttaca gccatagcac
    34441 gggtgaccaa tcgccaccgg ccacacaccg ccaacatcgc agaattgcag cgttgagcag
    34501 ttgccgtaag tttctggccc tttacagccg aggtggtaca agcaccagcc ttcgcggtgg
    34561 ccttcatcac cgaactcttt ggcaaaacga ccagcatcga agtgcgggcg acgttcgcag
    34621 tgttcgtgaa tcagacggcc ataggcgaag gtcggacggt ttttgtcatc cagtttcggc
    34681 ggtttgccgt aagtgatgat gtgcgcaacg gtcgcgagga agttgtgcgg gttcggcggg
    34741 cagcccggaa tattgataac ggttttgcct ggcagaactt cttgcaagct gactgcgcca
    34801 gttgggttaa ctccagctgc ggcaacaccg ccccacgcgg agcaggaacc aatagcgata
    34861 atggctgctg agccttccgc cgctttgcgg atgtgatcca caatcggctc accggcaacc
    34921 atgcaataaa taccgttatc ttttaatggg atggaaccat ccaccactaa cacatactgc
    34981 cctttgtact tctcgagagc gttatgtttg ttctcttcga cctgatgacc gaaggcggcg
    35041 gaaagcactt cgtgatattc cagagagata gtctccagca cgaggttttc taccgttgga
    35101 tgcgttgcac gaagcagaga ttccgtacaa ccggtgcact cctgcgcgcc aatccagata
    35161 actggcggac gctgcgggtt agtaaccgat tcggccatct ctgcagcggc tttgctactt
    35221 aaccccatgg tggcggctaa tgctgcacaa agcttcatga aatcacgacg gttaatgccg
    35281 tgagaatgga tgagggtgtt atctccagtc atttatagtt attccgttgc gagaacctgg
    35341 catatatttt gcctcaatcg caaaatcaat aatgcgatcg atgcgccatt taccacacat
    35401 ttattatggt tatcgatatc atgatactgc ggcggggcgc taaaacgaag ggaagaagca
    35461 ttagtgtaat taattaaagc aagataatac gtatgtttga tcaattttcg aggcgataac
    35521 cggccataaa aaagcgcaac gtagaacagg aattattctc acgaaagaaa tccattacca
    35581 tctctttatc cagtccatag cggcgcgtca gaatacccgc ttcccaggcg ctaagctggc
    35641 gatctccggt cttttcgtac atcctgtgtg agtagcctaa tacaaaaccg cgtttataat
    35701 cggcacagaa ttttgtcaca ttaaccgcac tgtcggcatg cgttgctttt aatcccgcca
    35761 ttaagccttt accaaaatgg ttattcatcg ttcacctcat tcgttatata tcgccatgta
    35821 taacgatatt ttaggcaaag aacgactttt gcgcacgcta ttttttcata cttgtttaag
    35881 acgtaatccc gtaagagata aacctatcag gagagatatt tatcgaacca tttcagtgtc
    35941 ctttgccagg caagatcggc ggcagatttg tcataacggg gcgtggaatc attatggaat
    36001 ccgtgattaa cccccggata gatataagcc tcataaacct tattattgtc tttcaacgcc
    36061 gcctcgtaag gaggccagcc ctcgttgatt cgggtgtcca gttccgcgaa gtggagtagt
    36121 aaaggcgctt caatcttcgc cacatcggca gtgggtgcct gacgaccata aaacggcacc
    36181 gcgcaggcca gttccggata cgcgacagcc gccgcgttcg atacgccacc gccatagcaa
    36241 aatccggtaa tacccacttt acctgtcgct tgcggatagc gttgcataaa ctcaattgcg
    36301 gcaaagaaat cattcatcag tttggttgga tcaacctgct gttgcagctc acgaccttta
    36361 tcatcatttc ccggataacc tccaacggaa cttaatccgt caggtgccag ggcgatatac
    36421 cccgccttcg ccactcgccg tgccacatct tcgatatacg gattcagtcc acgattctca
    36481 tgcaccaccc ccacggctgg cgttttgccg ctcatttttg cgggtttcac cagataaccc
    36541 cgtacctcgc cgtgaccatt tggcgaagga tacgtgatgt actcagcaac aatctccggg
    36601 tcggtaaact ctacctgagt cgccagcgca taatttggct tgagcaaatc aaacaacgct
    36661 aatgccgtca tcccgcccac cgcatacttc gccgcaaggt tgaggaactc acgtttcgaa
    36721 attttcccgt gaacgtaata gtcgtagtaa tccaacaact cttgtgggaa atctttggcg
    36781 gttaaacgcg gcatcgttgc attcctcaac tggtgttttt ttaagcaaag cataagcgcg
    36841 tgtttttgcc cagtttttcg tcactctgtg agccagacta cgggatacgc gctggcgaat
    36901 cgctaaacta gaaacattgt ttcgaaattg aacggtggaa aggagaggtc atggtctggt
    36961 tagcgaatcc cgaacgttac gggcagatgc agtaccgcta ttgcggaaaa agtggtttac
    37021 gcctgcccgc attatcgctc ggtttatggc acaatttcgg tcacgttaac gcgctggaat
    37081 cacagcgtgc aatcctgcgt aaagcgtttg atttaggcat tacgcacttt gatttagcca
    37141 acaattacgg gccgcctcca ggaagcgcag aagagaactt tggtcgcctg ctgcgcgagg
    37201 attttgctgc ttatcgcgat gaactgatta tctctaccaa ggctggctac gatatgtggc
    37261 ccggcccgta cggctctggc ggttcacgta aatacctgct cgccagcctc gaccaaagcc
    37321 tgaagcgtat ggggcttgag tatgtcgata tcttttactc tcatcgcgtc gatgaaaata
    37381 cgccgatgga agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt aaggcgctgt
    37441 atgtcgggat ctcctcttac tcgccagagc ggacgcaaaa aatggtcgag ttgctgcacg
    37501 agtggaaaat tccgctttta attcatcaac cttcgtacaa tttactgaac cgctgggtgg
    37561 ataaaagcgg cctgctggat accctgcaaa ataacggcgt gggctgtatt gcctttactc
    37621 ctctggctca gggattgctg accggaaaat atctcaacgg cattccgcaa gattcacgga
    37681 tgcatcgtga agggaataaa gttcgtggtc tgacgccgaa aatgcttacc gaagccaacc
    37741 tcaacagcct gcgcttattg aatgaaatgg cacagcagcg tggacaatca atggcgcaaa
    37801 tggcgttaag ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt ggtgccagcc
    37861 gcgcggagca actagaggag aacgtgcagg cgctgaataa tctgacattt agcaccgagg
    37921 agctggcgca gattgatcag catatcgccg atggcgagct gaatctgtgg caggcgtcgt
    37981 ccgataaatg acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga tcagtgatta
    38041 tgacgagtca gtcggtcaag atagcccatc acaaatgcag aaagcacaaa cgtcagatgg
    38101 ataatgacgt accacatcag tttgttatcc gggacatttt tcgcatccat aaagacgcgc
    38161 agtaagtgaa tggaagaaat tgccacaatc gacgctgcta ctttgttttt cagcgacgtt
    38221 gcgtccattt tccccagcca cttcagcttc tctttgttct cggagatatc cagctgtgag
    38281 acgaaattct cataaccgga aaacatcacc atcaccagca aaccgccaac cagcgtcata
    38341 tccaccagcg acagcaacac gaggatcaaa tctgattccg ccatcgagaa gatattcggc
    38401 agtacgtgaa taatctcctg gaagaacttc agcgccaggg caactaacgc cagcgaaagg
    38461 ccaaagtaca cgggggcaag cagccagcga gaagcataca ttgcattttc aagaaaacgt
    38521 tccatacatt cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt gagtaaacag
    38581 cacgcaacag caaatcaaca tatacgttaa acctttgtcg cataattctg aggtttaggg
    38641 atcttcctgg ttcccttatc aacgcatcga gagcgtctac ccttaaagga cgttgaacct
    38701 gaagggagaa aaacgatgtc tcatttaaaa gacccgacca cgcagtatta cactggtgaa
    38761 tatcccaaac agaaacaacc gacgccaggc atccaggcga agatgacacc gataccggat
    38821 tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc actggtgaca
    38881 gggggcgatt ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga aggggctgac
    38941 gtggcgatca gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa aaagatcatt
    39001 gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt taagcgatga gaaattcgcc
    39061 cgttcgctgg ttcacgaagc gcacaaggcg ttaggcgggc tggatattat ggcgctggtc
    39121 gccgggaaac aggttgccat tccggatatt gcagacctca ccagcgaaca gtttcaaaag
    39181 acctttgcca ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat ccccctgcta
    39241 ccgaaaggtg caagtgtcat caccacttcg tcaatccagg cataccagcc aagtccgcat
    39301 ttactggact atgcggctac gaaggcggcg attctgaact acagccgtgg cctggcaaaa
    39361 caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc caggccctat ctggacggca
    39421 ctgcaaattt ccggcggaca aacgcaggat aagatcccgc agtttggtca gaaaacgccg
    39481 atgaagcgtg cggggcaacc ggcggaactg gcccctgtat atgtttatct ggcaagtcag
    39541 gagtcgagct acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca tttaggttaa
    39601 aaaaatgccc ggttgtgaaa agcaaccggg catcattgtg aattcactct tacctgatgt
    39661 ggcgaaaacg ccttatccgc ctacaaacgc atgaagattc aacatgttgc aatagatttt
    39721 gtaggcctga taagcgtagc gcatcaggca ttgagcacct aatgccggat gcgtcgtgaa
    39781 cgccttatcc ggcctacaaa cgcacgaaga ttcaacatgt tgcaatagat tttgtaggcc
    39841 tgatgagcgt agcgcatcag gcaattctac tttacttcgc tttggcggtt tcttcgccga
    39901 ccagacctat cttcaggtaa cccgcctgat gcagcgtatc cattaccttc atcaacgtct
    39961 cgtaatcgac ggttttgtcc gctcggaaga agatggtggt gtctttcttg ccttcggtta
    40021 acgcattcaa cgccgtaatc attgtttcat cggtgaccgg atcgttaccg ataaacatcg
    40081 agttgtctgc cttcaccgac agataaaccg gtttttccgg gcgcggctgc ggcgtgctgg
    40141 tagaagcagg caagttcacc ttcacatcta ccgtcgctaa cggtgccgcc accataaaga
    40201 taatcagcag aaccaacatc acgtcgataa acggcgtcac gttgatatca tgcatttcgc
    40261 cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg acattatcct gcgcgtaatt
    40321 tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc cagatccagg tcacggcttt
    40381 gcagcaacaa tacctgcgct gcaacatcac ccagcatcgc tttaaagccg ccaatctggc
    40441 gtgcaaatac gttatagata acgaccgcag gaatcgctgc cacgaggccg attgccgttg
    40501 ctaacagagc ttctgcgata cccggcgcaa cgactgccag gttagtggtc tgcgtttgcg
    40561 cgataccaat aaagctgttc atgatgcccc agaccgtacc aaacagacca acgaacggag
    40621 aaatcgcgcc gatggttgcc agatagccgt taccgcggcc catttgacga cccactgcgg
    40681 cgacccgacg ctccagacgg aagctggtac gttctttaat accttcgtta tcgtcgctgc
    40741 cttctgacag ttccagctca ttctgcgctt cattgagcaa atgcaggctt aagcttttgc
    40801 taccaaaatc agcggcgata tcgttggcct ggtttaagga acgtgcttca gccagcagtt
    40861 gctgctcgcg cttaaggcga cgcttctgat tgaagaactc tacgctctta ctgaagaaga
    40921 ttgcccaggt gactacagag gccaaaataa gcccaatcat cacgcactta acgacaatat
    40981 cggcgtactg atacataccc cagacggaaa ggtccgtctg cattaaatta ttacccactg
    41041 tgtatctcca ggacgcaagt cacaaaatct gcgcataata atatcaaaac gacgtcgaat
    41101 tgatagtcgt tctcattact atttgcatac tgccgtacct ttgctttctt ttccttgcgt
    41161 ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt tatgccaatt
    41221 cttcaggatg cgcccgcgaa tattcatgct agtttagaca tccagacgta taaaaacagg
    41281 aatcccgaca tggcggacaa aaagcttgat actcaactgg tgaatgcagg acgcagcaaa
    41341 aaatacactc ttggcgcggt aaatagcgtg attcagcgcg cttcttcgct ggtatttgag
    41401 agtatggaag ccaaaaagca cgcgacgcgc aatcgcgcca atggcgagtt gttctatgga
    41461 cggcgcggaa cgttaaccca tttttcctta caacaagcga tgtgtgaact ggaaggtggc
    41521 gcaggctgcg cgctatttcc ctgcggggcg gcagcggttg ctaattcgat tcttgctttt
    41581 gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct atgaaccgag tcaggatttc
    41641 tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt ggtttgatcc gctgattggt
    41701 gccgatatcg ttaagcatct gcagccaaac actaaaatcg tgtttctgga atcgccgggt
    41761 tccatcacta tggaagtcca cgacgttccg gcgattgttg ccgccgtgcg cagtgtggtg
    41821 ccggatgcca tcattatgat cgacaacacc tgggcagccg gtgtgctgtt taaggcgctg
    41881 gattttggca tcgatgtttc tattcaggcc gccaccaaat atctggttgg gcattcagat
    41941 gcgatgattg gcactgccgt gtgcaatgcc cgttgctggg agcagctacg ggaaaatgcc
    42001 tatctgatgg gccagatggt cgatgccgat accgcctata taaccagccg tggcctgcgc
    42061 acattaggtg tgcgtttgcg tcaacatcat gaaagcagtc tgaaagtggc cgaatggctg
    42121 gcagaacatc cgcaagttgc gcgagttaac caccctgccc tgcctggcag taaaggacac
    42181 gaattctgga aacgagactt tacaggcagc agcgggctat tttcctttgt gcttaagaaa
    42241 aaactcaatg atgaagagct ggcgaactat ctggataact tcagtttatt cagcatggcc
    42301 tactcgtggg gcgggtatga atcgttgatc ctggcaaatc aaccagaaca tatcgccgcc
    42361 attcgcccac aaggcgagat cgattttagc gggaccttga ttcgcctgca tattggtctg
    42421 gaagatgtcg acgatctgat tgccgatctg gacgccggtt ttgcacggat tgtataacat
    42481 tgccactttt ggacaatttt gcagacattt tattgtgaaa agtcttaaat tgttgcgtcc
    42541 gggatcaagg cgtcccggac gattcaggag tacaataggc agataaaggc ttaaacgctg
    42601 ttccacagga aagtccatgg ctgttattca agatatcatc gctgcgctct ggcaacacga
    42661 ctttgccgcg ctggcggatc ctcatattgt tagcgttgtt tactttgtca tgtttgccac
    42721 gctgttttta gaaaacggcc tgctgcccgc ctcatttttg ccaggcgaca gcttgttgat
    42781 actggcaggc gcattgattg cccaaggggt tatggatttt ctgcctacga ttgcgattct
    42841 gaccgccgca gcaagtctgg gctgctggct aagttatatt caggggagct ggttagggaa
    42901 taccaaaacg gtgaaaggct ggctggcaca gcttcctgct aaatatcacc agcgcgccac
    42961 ctgcatgttt gaccgccacg gtctgctggc gctgctggct ggacgttttc ttgcatttgt
    43021 ccgtacgctg ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc gcttccagtt
    43081 tttcaactgg ttaagtggat tgctgtgggt cagcgtggta accagttttg gctatgcctt
    43141 aagtatgatt ccgttcgtta aacgccatga agatcaggta atgacgttcc tgatgatcct
    43201 gccaattgcc ttgttaaccg ctggcttgtt aggcacgctg tttgtggtga ttaaaaaaaa
    43261 atactgtaac gcctgacgat tttccccgtt cccggctgct gtaccgggaa cgtatttaat
    43321 tcccctgcat cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg aagtaacgtt
    43381 taaactcacg gctaaattgc gatgcgcttt catagccgac gcgcatcgct gctgcgctgg
    43441 ccttcatgcc gtcatggatg atcatcatcc gcgccttatg cagacggtaa ttcttcaaat
    43501 actgcaacgg cgaggtgctg gtgacagact taaaattatg gtggaacgcc gatacgctca
    43561 tgttggcttc tgccgccagt tgctcgacgc tcaggttttc ggtgtattta ttctcaattc
    43621 gtttcagcac gcggctaatc agactgaagt gagtctggcg actgaccagc gccagtaacg
    43681 cgccgccgca aggtccggtc agcacgtagt acagaatttc gcggatgatc tgtttgccga
    43741 gaatacgcgc atccagtggt cgctccatca cgtcgagtaa ccgctccgcc gcgcataaaa
    43801 tctcttctga taacgtggcg gagttaatcc cgctggctgc catcgacggc tggaaatgct
    43861 catcttcgcc aatgtccatc aacagttcct gtaactgcaa aatatcgaca ttgagacgca
    43921 accctgccag cggcacctct gacgtggcat aggtttcgca ctcaaacggc aacggcaccg
    43981 tcagcagcag gtattcattg gcatcataac gaaacacgcg ttcattgata taaccgattt
    44041 tatgcccgga aaagagaatt atgatgccag gctcgtacat caccggtgta cgtgcgaaag
    44101 gcgtctcgcc atacaacaaa cgcacatcgg gcaacagttc tgacaaacta ttttctttat
    44161 ttttcagttt attaacttta tccgccagca agcgacaaat ctcttcacgt ttcatatcgc
    44221 gtaatttctt aggaataatg cggcaatttg attgtgcgca attttgtagc atttctccag
    44281 cactctggag aaataggcaa gacattggca gaaatgagca ttgagagcca gggcgctggc
    44341 gatcacaatg aaaaacatca ggcagatcgt tctctgccct catattggcc cagcaaaggg
    44401 agcaagtaat gaacaacttt aatctgcaca ccccaacccg cattctgttt ggtaaaggcg
    44461 caatcgctgg tttacgcgaa caaattcctc acgatgctcg cgtattgatt acctacggtg
    44521 gcggcagcgt gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg aaaggcatgg
    44581 acgtgctgga atttggcggt attgagccaa acccggctta tgaaacgctg atgaacgccg
    44641 tgaaactggt tcgcgaacag aaagtgactt tcctgctggc ggttggcggc ggttctgtac
    44701 tggacggcac caaatttatc gccgcagcgg ctaactatcc ggaaaatatc gatccgtggc
    44761 acattctgca aacgggcggt aaagagatta aaagcgccat cccgatgggc tgtgtgctga
    44821 cgctgccagc aaccggttca gaatccaacg caggcgcggt gatctcccgt aaaaccacag
    44881 gcgacaagca ggcgttccat tctgcacatg ttcagccggt atttgccgtg ctcgatccgg
    44941 tttataccta caccctgccg ccgcgtcagg tggctaacgg cgtagtggac gcctttgtac
    45001 acaccgtgga acagtatgtt accaaaccgg ttgatgccaa aattcaggac cgtttcgcag
    45061 aaggcatttt gctgacgcta atcgaagatg gtccgaaagc cctgaaagag ccagaaaact
    45121 acgatgtgcg cgccaacgtc atgtgggcgg cgactcaggc gctgaacggt ttgattggca
    45181 ctggcgtacc gcaggactgg gcaacgcata tgctgggcca cgaactgact gcgatgcacg
    45241 gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat gaaaaacgcg
    45301 agaccaagcg cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc actgaaggtt
    45361 ccgacgatga gcgtattgac gccgcgattg ccgcaacccg caatttcttt gagcaattag
    45421 gcgtgccgac ccacctctcc gactacggtc tggacggcag ctccatcccg gctttgctga
    45481 aaaaactgga agagcacggc atgacccaac tgggcgaaaa tcatgacatt acgttggatg
    45541 tcagccgccg tatatacgaa gccgcccgct aagcttttta cgcctcaaac tttcgttttc
    45601 gggcatttcg tccagactta agttcacaac acctcaccgg agcctgctcc ggtgagttca
    45661 tataaaggag gaacgtatgg ctaatccaac cgttattaag ctacaggatg gcaatgtcat
    45721 gccccagctg ggactgggcg tctggcaagc aagtaatgag gaagtaatca ccgccattca
    45781 aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc gcggcctaca agaacgaaga
    45841 aggtgtcggc aaagccctga aaaatgcctc agtcaacaga gaagaactgt tcatcaccac
    45901 taagctgtgg aacgacgacc acaagcgccc ccgcgaagcc ctgctcgaca gcctgaaaaa
    45961 actccagctt gattatatcg acctctactt aatgcactgg cccgttcccg ctatcgacca
    46021 ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa gagggattaa tcaaaagcat
    46081 cggcgtgtgc aacttccaga tccatcacct gcaacgcctg attgatgaaa ctggcgtgac
    46141 gcctgtgata aaccagatcg aacttcatcc gctgatgcaa caacgccagc tacacgcttg
    46201 gaacgcgaca cacaaaatcc agaccgaatc ctggagccca ttagcgcaag gagggaaagg
    46261 cgttttcgat cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa ccccggcgca
    46321 gattgttatc cgctggcatc tggatagcgg cctggtggtg atcccgaaat cggtcacacc
    46381 ttcacgtatt gccgaaaact ttgatgtctg ggatttccgt ctcgacaaag acgaactcgg
    46441 caaaattgca aaactcgatc agggcaagcg tctcggtccc gatcctgacc agttcggcgg
    46501 ctaacgtgca aattctcccg gtggcggtaa tgttccgcta ccggactttt cagaaatcat
    46561 ttattcccct cgcgtcccgc ccgttgttac tcttccttgt tcaggaatgc caaatataag
    46621 gacatcatca tgcagagccg gaagctctta aaagaacaac tcatctatat ccgggataaa
    46681 cgcaacggag aggtgaaaaa cagatgaaaa taatacttct gtttttagca gccctggcaa
    46741 gttttaccgt acacgcgcag cctccttcac agaccgtaga acaaacagtc cggcagattt
    46801 atcagaacta taaatcagat gccagtacgc cttatttcgg tgaaaccgga gagcgggcga
    46861 taacttcagc gcgtattcaa caggcgctta ccctgaacga caatcttaca ctaccgggca
    46921 atattggctg gctggattat gatccggttt gtgattgtca ggattttggc gatctggtgt
    46981 tagaaagcgt agcgataacc caaactgacg tcgatcatgc cgatgccgtt gtgcgctttc
    47041 gtatctttaa agatgataaa gaaaagacca cacagacact gaaaatggtg gcggaaaatg
    47101 gtcgttgggt cattgatgat attgtcagca atcatggcag cgtcttacaa gcagttaata
    47161 gcgagaatga aaaaacgctg gccgctttag cttcgttgca gaaagaacag ccagaatcct
    47221 ttgttgctga actctttgaa catattgctg attatagctg gccgtggacg tgggtggttt
    47281 ccgactctta ccgccaggcg gttaatgcct tctataaaac caccttcaag acggccaata
    47341 atcccgatga agatatgcaa atagaacggc aatttattta cgacaatccg atctgttttg
    47401 gcgaagagtc gctattttca cgcgttgatg aaattcgagt cctggagaaa accgccgatt
    47461 ccgcccgcat tcatgttcgt tttacgctga ccaatggcaa caatgaagag caagagctgg
    47521 ttttacagcg gcgcgaaggt aagtgggaaa tcgctgattt tatccgcccg aacagcggca
    47581 gcctacttaa gcagattgag gcaaaaaccg ccgccagatt aaagcaatga gctgaattaa
    47641 ataacaatta gccggaacaa taaataaaag ggaacactat atgaaaacga ttttcaccgt
    47701 gggagctgtt gttctggcaa cctgcttgct cagtggctgc gtcaatgagc aaaaggtcaa
    47761 tcaactggcg agcaatgtac aaacattaaa tgccaaaatc gcccggcttg agcaggatat
    47821 gaaagcacta cgcccacaaa tctatgctgc caaatccgaa gctaacagag ccaatacgcg
    47881 tcttgatgca caggactatt ttgattgcct gcgctgcttg cgtatgtacg cagaatgata
    47941 aaaaaatccc cggcagcatg tcagttgccg gggatttttt ttaacgtcca accgccgctt
    48001 tagggcgttt cttcgcacca gcattcaccg gacgagattg cgtagacgac gctttttttg
    48061 ccgtagcagg cgtctgacgc tgagtcgcca tcggcgtatg tttagtcaac gccggacggg
    48121 tattgcggtt ctggcgacga gcttcacgca tctcttcaat ggttggcgca ggcactaagc
    48181 aatcgcgacg gctgccaatc agatgctttt tgcccatcgc ttccagcgcc tggcggatta
    48241 acggccagtt tgccggatcg tggtaacgca acaacgcttt atgcaaacga cgctgtttgt
    48301 cgcccttcgg tacgaagacg ccttcgctct tataaccaat cttcgccagc ggttttttcc
    48361 cggtgtaata catggtggtt gagttcgcca gcggcgatgg gtagaagttc tgtacctggt
    48421 cgagacggaa acgatgcttt ttcagccaca gcgccaaatt caccatatct tcatcacgcg
    48481 taccggggtg cgcagagatg aaatacggga tcagatactg ctctttacct gcctgtttcg
    48541 agtaggtatc gaacagctct ttaaagcggt catagctgcc catgcccggc ttcatcatct
    48601 tcgacaacgg cccttcttcg gtatgttccg gagcaatctt cagataaccg ccgacgtgat
    48661 gggtcgccaa ctctttgata tagcgcggat cttccacggc gatgtcataa cgtacgcctg
    48721 aggcgataag gattttttta atgcctttca gatcacgcgc acggcgatag aggttgatcg
    48781 tcggttcgtg gttagtgtcc atgtgcggac aaatatccgg ataaacgcac gacaaacggc
    48841 gacaagtttg ttcagcgcgt ggcgatttgc agcgcaacat atacatgttg gcagttggcc
    48901 caccgagatc ggaaatcacg cccgtaaaac ctggaacggt gtcgcggatc gcttcgatct
    48961 cattaatgat cgaatcttcc gaacggctct gaataatgcg cccttcgtgc tcggtaatag
    49021 aacagaaaga acagccgcca aagcagccgc gcataatgtt gaccgaaaaa cggatcattt
    49081 cgtaagccgg aatacgggca ttgccatagg ccggatgtgg cacgcgcttg tacggcagcg
    49141 caaaaacgct gtccatctct tcggtagaaa gcgggatagc aggcgggttg atccacacat
    49201 agcggtcacc gtgtttttgc atcaatgcgc gggcgcagcc tgggttggtt tcgtggtgca
    49261 gaatgcgcga agcatgggcg tacagcactt tatcgccctt cactttctcg aaagaaggca
    49321 gcaatacgta ggttttttcc cacggtttct ggcgcggtgg ctgcacgatt acagctttcg
    49381 cttcctgctt tttcggcgca acaggtttgt tatccgcaca cggcaaatct tcgccatacg
    49441 gatgcgggat tgggtcgatt ttccctaggg tatcaagacg ggtggaatcc acaccgctcc
    49501 agccaggcag cgcctctttt acgataattg cggtattacg cacatcgcgg atttcactaa
    49561 tcggctcgcc catcgccaga cgatgcgcta cttccaccag cggacgttca ccgttaccaa
    49621 acatcagcat gtcggctttc gaatctacca gaacggaacg gcgcacggta tcggaccagt
    49681 aatcataatg cgcggtacgg cgcaggctag cctcaatacc gccgaggatc accggcacat
    49741 ctttccacgc ctctttacaa cgctgggtat aaaccagcgt ggcgcgatcc ggtcgcttac
    49801 ccgcgacatt atctggcgtg taggcatcat catgacgtaa acggcgatcg gcggtatagc
    49861 ggttgatcat cgaatccatg ttgccagcag taacaccaaa aaacagattc ggtttaccca
    49921 gacgcataaa gtcgtctttg ctgctccagt ccggctgggc gatgatcccg acgcgaaagc
    49981 cctgcgcttc cagcatacga ccgcaaatcg ccatcccgaa gcttgggtga tcgacatacg
    50041 cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc aagttgatcc atctcttcac
    50101 gagacatcgg caaaaacggt gccggtccaa aacaggcggc ccagtactgc ggccaggaga
    50161 acaggtcgcg atccggttgg atcagggaga tagagctcat tttgcttcca gaaatgataa
    50221 aaaaataatc aaaggccggg gattataagc cggaacgaaa gagaaatcga aaggtattcc
    50281 atactcgccc tcctcgggcg agtatgaaga ttacggtacc ggattgacca acagttgccc
    50341 aatcgagcca cggtccgcca tttccagcgt ctgactgttg aagtagaacg ggaagtgcgc
    50401 ccaggaaggc tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc aaacggtatc
    50461 tttccagcct ctgtcttccg ggaacggcat cgcgccattc acgttacgga tctggaacat
    50521 tacgccttca atatggaacg cttgcggctc gtccgcgcgt accgtccagc gttcccacgt
    50581 tccttgctgc gcggtgacat caatacggtt gacgtcccac agctgtccat taatacccgg
    50641 gtcatcaccc agactgatat cgcgactgcg aattggcgaa ccagccatga tttcagttgg
    50701 cagcaagcgc atcggaagac tgtctgtgac cagcggcaga aggccggttg ggcgcagcgt
    50761 tagcaccaag gtagaaacca gaatactgga tggctcaaag aagccgcgaa tacgatcaac
    50821 aatgctcgcc gcttcaccac aggtgatcga cacttcatcg ccgttgctca tatccaccag
    50881 aatctcgcgg cgctcgcccg gtgccagcga aagttgcttc accgatacag gagcaggcag
    50941 gaatccctga tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct gtagttgata
    51001 gcgacgagag ttcgacgcgt tcagcagtcg caagcgcacc cagccacgcg agacttcaac
    51061 gtacgggctt tgtacaccgt taaccagcag cgtatcacca acaaagccgc cgcttcccgg
    51121 ttcgttgtat tctggcgtac caaagttatc cagccgttta tcctggataa tgaccggaaa
    51181 atcatccaca ccataatggt tggggatagg cagcgacttg ctgacttcat cttccaccag
    51241 ccacattccg gcaaggccgt tatagacctg ctgagccgtg cggttgggag tattggcgtg
    51301 ataccacaga gtagctgcgt tctggcgaat aggcagtacg ggtgcccagt cagcgtttgg
    51361 cgacatcatc cgtgccggac cgcccatcag cgggcctggt acctgtagcc cggccaccgt
    51421 cattgagaca ttttctgtca ggcggttgct gtaaataagc ttaacatcgt cgcccttcca
    51481 gacgcggata gtcggcccca ggtaacgacc attgattccc cagaccgacg cgcgtgtccc
    51541 tggcgtaaat gaccagtgcg cacgttgtac agtcataaac agcggttgcc cacggcgaga
    51601 ttcaagtagc ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg ccttcagggg
    51661 aacagcgcct gcacaaagtg caatccccga tgcctgaatg aactgacgcc gactgagtga
    51721 catatttgct ccatgtaaaa ctggctaaag taataccgtg caggaaagtg ttccccgcaa
    51781 aaatacagat tgccgactta aacttttccg gcggcttcgc gttctgcgac ttctttatcg
    51841 agctcggcga ttttttgttc cattatcgaa cgacaatggg cagccagctc acgaacctga
    51901 tctttgccat actgactgac gtcaattggc ggcagcattt cgacaatcac cagaccgttg
    51961 tgcagtcgat taagattaat cttattcgaa gttgtagaga cgcacacggg aataatcggg
    52021 acgcccgccg caattgccgc gtgaaatgct ccagtcttga acggtagcag gccgcgacca
    52081 cggctgcggg ttccttccgg gaacatccag atggaaatac ggcgtttttt gaaatgattc
    52141 acgacttccg caatggtgcc gtgagcttta gtgcgattgt ttctgtcgat caataagttg
    52201 ccggttaacc agtacaactg cccgaagaag gggatccaca gcaagctctt tttacctacc
    52261 gtcaccgtcg gcggttgcac aatgttcgat gctgtcacca tgtcatagtt gttctggtgg
    52321 ttagcgatat agatagcatt gccgtagctt tcagcatctg caggtttgcg gcactcaact
    52381 ttcaggccga acagtggcgc aagacggcca aacatatgcc caaaggtggc cacatgtttc
    52441 gggttacgcg ggctgaaaag gcagtaaatg gagccgaata cacagactaa gatgctgtaa
    52501 atcacggtaa taataagacg aaagatatat agcataacac cctctgaagg tctgagagac
    52561 tggcattgta cgtcacctga aatcaggtta gggcattgtt aaaagccgct ctctggaaaa
    52621 gcgtattgtt aatcgcaacg catgaataaa caacggtttc aagggaattt ttatcgtttc
    52681 cgggggttga atacgctgcc ggatgacgac ttaacgtttc atccggcgtt ccttgctagc
    52741 gggaggaaac agcgccctcc ccggcatatt actcttcgct atcaccgctg ctggcacggc
    52801 gaggagagtc gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc
    52861 gtccacgttc gccagtgact ttctgtagct cttccggacg cagtttaatt ttgcgtttcc
    52921 caacatgaat ggtcagcgtg ctttgcggcg gcagaacgta cagttgcgcc agaccatcct
    52981 cgccacgcgc ggcttctgcc gatggaatgt tgataatctt gttgcctttg cccttcgaca
    53041 gctgcggcag atcgcttacc gggaacatca acatacggcc tgcctgagtg attgccagca
    53101 gcatatcgga agcatcttca atcaccaccg gcggcataac atgggcattt tccggtaagg
    53161 tgatcaaagc cttacccgca cggttacgcg ctaccagatc gttaaaggtg cagacgaaac
    53221 cgtaacccgc atcggaagcc atcagcagtt tctgatcgtc gctttccatc agcatatggt
    53281 caacggtcgc cccaggcggc aacgttaatt tgccggtgag tggttcgccc tgaccacgcg
    53341 ccgacggcag cgtaatcggg tcgatggcat agctacgacc ggtggaatca acaaacacta
    53401 ccggttggtt actcttacct ttcaccgccg ctttgaagct atcacccgct ttataattca
    53461 ggcccggcgc gtcgatatca tggcctttag cgctgcgtac ccagcccatc tgcgacagca
    53521 caatggtgac aggttcagac ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc
    53581 gttcctgcaa cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt
    53641 tcttcagcag gttattcatt ttacgctcgg aagccaaaat gccctgcaac tggtcgcgct
    53701 ctttttccag ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac
    53761 gcagtttcag ttcgaggatc gcttccgcct gggtttccgt aaggccaaac cgcgacatca
    53821 gcgccggttt cggttcatct tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa
    53881 acgccaccag caaaccttcc aggatatgca ggcgcttgag gactttctcc agacgatagt
    53941 tcagtcggcg gcgcacggta tcgcgacgga acaccagcca ttcggagagg atttccagca
    54001 ggtttttcac cgccggacga ccatccagac cgatcatatt gaggttaatg cgatagctct
    54061 tttccagatc ggtggtagcg aagaggtggt tcatcacctg atccatatcc acgcggttgg
    54121 aacgcggcac aatcaccaga cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt
    54181 caaccatcgg cagctttttg ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac
    54241 ctgaaacctg atgcggtaat gcgctgataa ccaccgcgcc atcttctttc ttccacaccg
    54301 cgcgcatacg cactgaacca cgtccgttct cgtagatttt acgaatctcg gcgcgcgaag
    54361 tgataatttc cgcttcagtc ggataatccg gcccctgcac gatatccagc agctgatcga
    54421 gcgtagtttt cggctggtcg attaatgcga ttgccgcctg agctacttca cgcaggttat
    54481 gcggtggaat atcggtcgcc atgccgacgg caataccggt ggtgccgtta agcaaaatgt
    54541 ttggcagacg ggcaggtagc attttcggct cctgcaaagt gccgtcgaag tttggcaccc
    54601 agtcagccgt cccctgcccc agctcgctca atagcagctc ggaatatttc gacaaccggg
    54661 attcggtgta acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct
    54721 gaccatcaac cagcggataa cggtaagaga acggctgcgc catcaggacc atcgcttcat
    54781 aacaggcgct atcgccgtgc ggatggtatt tacccagtac gtcaccgacg gtacgggccg
    54841 attttttaaa tttggcgctg gcattcaggc ccagttcaga catcgcatac acaatgcggc
    54901 gctgaacagg tttcagacca tcaccaataa acggcaacgc acggtccatg atcacgtaca
    54961 tggagtagtt taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc tctgccatat
    55021 cgctcattaa ttctgattcc tcaacttatt cgccagcctt caaaggggca atattgccgg
    55081 agatactaca ttatctgccg cgtcgagtca caggagatca gcgtgaatag cgccgccgga
    55141 cgtgctccga cggcgataaa ggcatcaatg tgccttgata tacaactctt tgctgtagac
    55201 ttgatcggca tagagggcag cacaaggttg cgcccatgcc acaccactcg gcatgggggt
    55261 ccgctccggg cgattcgaga agttgaggtt atgcgagtcc agggaagcct aaagcatcaa
    55321 agtagtcgac tgtaagtata cggttcagcc cagtttagct gtgtctccac cagtgccccg
    55381 cgcctcctgc cataagtgcg gcttgctcat ggatatcccc gagacggctt acggcgcacc
    55441 ggataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa gcccaacaac
    55501 aatacgcctg tgacattgtt cgctcaaact tatcgcgagt gatttggctc acactccggt
    55561 aaagattccc catcacgcca acggcacata aacatccgtt tccagtcgct gctctgtcac
    55621 cccttcggca agattggtgt agtgaaacag aatcggtgct ttacgcattt tctcgccgct
    55681 tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt ccagctcgcc
    55741 aacgtggcgg gccacggcat aacgtccacc ggtaagttca ccattgctca caccataacg
    55801 attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg cttccggtgc
    55861 ggtggtggca ggggcgtccc aggcgacgcc aaaagtctgg ctttggttaa caggagaaag
    55921 ccccgtctct ttacgccaca tgataaattt cgccacactg tagttgagca actcagggct
    55981 acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt ttacatccag
    56041 tgtcaggttt gtcattttgc ctccctgcga aattattccc gggaacgcgt cccgggagcg
    56101 gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca gaattccagt
    56161 ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc
    56221 agcgtttgtg atcgatatca acattgatag taccgctggc atctttaaac acgtagagat
    56281 cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg tcacgcaggg
    56341 attttgcgct ttctacagtc gttacgctgc cgttcggccc ctgaaatcct ccggcctgac
    56401 tttgcgttac cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc
    56461 acagggccat tactgcgatt actgctgcga attttttcat gtttattact ccctttaatg
    56521 tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa aaaaataaaa
    56581 tttaatgctg tacagagcgc gttacaacac ggtttactgg cagcaaatac ggttatcgca
    56641 gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggt gacggcatca
    56701 aaactggcct tagtaaaatg ggttttagtg tcgactggtt tacacaaggt cgtcagggaa
    56761 aagaggcgct ttatagcgca ccttatgatg cggtgatcct ggatttaacc ttaccaggca
    56821 tggatggtcg cgatattttg cgcgaatggc gagagaaagg tcagcgtgag ccggtactga
    56881 acctgaccgc gcgtgatgcg ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg
    56941 attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggag gctctgatgc
    57001 gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg
    57061 gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa gaatttgccc
    57121 tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg attgaagaga
    57181 aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc
    57241 tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc tacacattag
    57301 gtgagaaatg aaatttaccc aacgtcttag tctgcgcgtc aggctgacgc taatcttttt
    57361 aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac aaacaacgga
    57421 taacgtcgat gaattgttcg acacccaact gatgctgttt gccaaacggt taagtacgct
    57481 cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata aattaaaaca
    57541 cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg gcagaatggt
    57601 ccttaatgat ggcgataacg gagaagatat tccctatagc tatcaacggg aaggttttgc
    57661 tgacgggcaa ctggtcggtg aagacgatcc ttggcgtttt gtctggatga cctcacctga
    57721 tggcaaatat cgcatcgttg ttggccagga atgggaatac cgtgaagaca tggcgctggc
    57781 gattgttgcc gggcaattga tcccgtggct ggtcgcactg ccgattatgt taatcatcat
    57841 gatggtacta ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat
    57901 gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg aagtgcggcc
    57961 actggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg
    58021 acgctttacc tccgatgcag ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca
    58081 aaccgaagtt gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag cactgctcca
    58141 attacattcc gggatcgatc gcgctactcg tctggttgat caactgctca cgctatcgcg
    58201 gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag atctcctaca
    58261 atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg tgcgactgac
    58321 actcaatgcc cacggcatca aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg
    58381 aaatttgctg gataacggcc ccggtgtgac accagaggca ctggcgcgaa ttggcgaacg
    58441 cttctatcgc ccacccggac aaaccgctac cggcagtgga cttgggctat cgattgtcca
    58501 gcgaatcgcc aagctgcatg acatgaatgt tgagtttggg aatgcggaac aaggtggatt
    58561 tgaggcgaag gtaagctggt aacttctctg tctatgagag ccgttaaaac gactctcata
    58621 gattttacta atagcaaaat ataaaccgtc cccaaaaaag ccaccaacca caaaccagac
    58681 aaataacgag cgagaataca tatagccaaa actcgaacat ttcccgcata aagagttttc
    58741 ttaagataag aataataagt ggcgtaagaa gaaaaaatgc tgcaatcatt gcttgcaata
    58801 tacgatcttc gcctgaaacg gaaggtatac tgcaaatatt gcctcccgac tcaagcatcc
    58861 agtcatactt attgaaagca aaaaaagaat ctattaacca ataagtaagc attatatata
    58921 gcaatgcttc aaatattgtt ttaattttct gctttaacat atatcagtac gctcataaaa
    58981 atccatttat aattatcaac atgacacgtt gatattttca tcttttactt attcaaagcc
    59041 atattttcaa tccccaaaaa aaccgaacca aacgtgtaaa ttattccggt gcgagtaaga
    59101 attatgagga atggctatca gtattgtcat tttcagaaaa tatttatcct gcatcggtga
    59161 gtcagagcta gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt
    59221 cacagtaccg ctctgtgacg tactatagtc gacaaacgtc tcaccttgag attaaaaaat
    59281 gagcaacatc ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa
    59341 cgacaccctg accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat
    59401 cgttcgcgcc gacagcgact acaatgtcaa agcggaagta caaaactttc tctgggctga
    59461 tgtggtgatc tggcagatgc caggctggtg gatgggcgcg ccgtggacag tgaaaaaata
    59521 tattgatgat gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg
    59581 caaagatccg tcgaaaaaat acggttccgg cagcctggta caggacaaaa aatatatgct
    59641 ttctctaacc tggaacgcac caatggaagc cttcaccgaa aaagatcagt tcttccacgg
    59701 cgttggcgtt gacggtgtgt atctgccgtt ccataaagca aaccaattcc tcggtatgga
    59761 accgctgccg acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac
    59821 tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa ctagagctca ggctttagaa
    59881 ggagttaacc atgcttaccg taatcgcaga aatccgtact cgtcctggtc aacatcaccg
    59941 tcaggcggta ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg
    60001 ccacggctat gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc
    60061 ggattctatc gtgatgattg agcagtggga aagcatcgcg caccttgaag cgcatctgca
    60121 aaccccgcac atgaaggcgt acagcgaagc cgtaaaaggc gacgtgctgg agatgaatat
    60181 ccgtattctg cagccaggga tttaatcctg ccttgtttgc ccggccatcc tgaccgggca
    60241 atgttctttc ctttaaacct caatctccgc catgtcgcct ttctcttgca accagttgcg
    60301 gcgatcttcc gagcgtttct tcgccagcag catatccatc atcgcgtcag tacgctgatc
    60361 gtcttcatca tcgatagtca actgcaccag acggcgagtg ttcggatcaa gcgtggtttc
    60421 acgcaattgc atcgggttca tttcccccag acctttaaaa cgctggacgt tcggcttgcc
    60481 tttcttgcgt tttaattgct caagtacgcc ctctttctct tcttccgtca gcgcgtaata
    60541 aacctctttc ccgagatcaa tacggtagag cggtggcagt gcgacgtaaa cgtgaccgtg
    60601 tttcaccaac gcgcggaagt gttttacgaa caaagcgcag agcagcgtgg caatgtgcag
    60661 accatcagag tccgcatccg cgaggataca gattttgcca taacgaagct ggctcagatc
    60721 gtcgctgtca ggatcgatac cgatcgctac cgaaatatca tgcacttcct gcgaggccag
    60781 cacttcgtcg gaagaaactt cccaggtgtt aaggatctta cctttcagcg gcatgatcgc
    60841 ctgatattca cgatcgcgcg cctgcttggc agatccgcct gcggagtcac cttccacgag
    60901 gaacagttcg gtacggttaa ggccctgcgc ggtacaatca gccagtttgc caggcagcgc
    60961 cgggccgctg gtcagctttt tacgcaccac ttttttagcc gcacgcatac gacgctgggc
    61021 gctggaaatc gccatctctg ccagcaactc cgccgcctga acgttctggt tcagccacag
    61081 gatgaaggca tctttcacca cgcccgaaac gaatgccgca cactgacgcg aagagagacg
    61141 ctctttggtc tgcccggcaa actgcggatc ctgcatttta accgacagaa cataggcgca
    61201 gcgatcccag atatcttccg ccgacagctt cacgccgcgc ggcagaatgt tgcggtattc
    61261 acagaactca cgcatcgcgt ccaacaggcc ctgacgcaga ccattaacat gggtaccgcc
    61321 ctgcatcgtt gggataaggt tgacgtagct ttcggtcagc agttcaccgc cttccggcag
    61381 ccacagtagc gcccagtcca cagcttcagt atcgccagcg aaattaccga taaacggttt
    61441 ttctggcagc gtcggtaaac cgtttaccgc ttccgccagg taatcattca gaccgtcctg
    61501 atagcaccag cgctgttcgg tgttgttgat ctcatcttta aaggtaatct caacgccggg
    61561 gcacagtact gctttggcct tcagcacatg cgtcaggcgt gaaacagaaa aacgcgggct
    61621 gtcaaagaag gtttcatccg gccagaagtg cacgctggta ccggtattgc gtttaccgca
    61681 agtgccgaca acctgtaaat cctgcacctt ttcgccattt tcaaaggcga tgttataaac
    61741 ctgaccatcg cggcgcacgt taacttctac gcgcttcgac aaggcgttaa ctaccgaaat
    61801 ccccacgcca tgcaggccgc cagagaactg gtaatttttg ttagagaatt taccgcctgc
    61861 atgcagacgg caaagaatca gttcaaccgc cggtacccct tcttccgggt gaatatccac
    61921 cggcatcccg cgcccatcgt caatcacttc taacgactgg tcagcatgta agattacatc
    61981 cacgcgtttt gcgtgacccg ccagtgcttc atcgacactg ttatcaataa cttcttgccc
    62041 caaatggtta gggcgagtgg tatcggtata catccccgga cggcggcgaa ccggctcaag
    62101 cccggtgagt acctcaatgg catcagcgtt ataagtttgc gtcatggttt aagttagcaa
    62161 ttcgagttga tcgtcagaga tggtgcagac caagaaaatc gacgatcggg ttgaaataat
    62221 cttcgaagcc cgtgaatgcg tggttgccgc cttctatgac agtctggcgg caggaagcat
    62281 agtacgccac cgcctggcgg taatccagca cttcatctcc cgtctgttgc agcagccaga
    62341 tcaaatccgg cgcttccagc gggtcaatct gcatgacttt gagatcgtaa atatggcgtg
    62401 actctagcac atattgctgc ccggtgtagg ggttctcgtt ctgaccgaga tagtccgtca
    62461 gcaattcaaa cgggcgtacc gccgggttta ccaccactgc gggcagcata aaacattgcg
    62521 acaaccaggt ggcgtaatat ccccccagtg acgaaccgac aatacccagc gaatcgccgc
    62581 catgttccag gacaatggat tccagcagtt ctgccgcgtc ggaaggatac ggcggcaact
    62641 gcggaatgat catctcaacg tcagggtgat gttccgccag ccagtttttt aacaagctcg
    62701 cttttgcaga gcgcggcgag ctgttgaaac cgtgtaaata aagaagcgta gacatcagta
    62761 gccttctgaa gcggtatcag gttggaaacg tgtgtccgcc aggcgatgca cctcggtggt
    62821 cagcgtgcca tcagcatgta actcgagagt acgccagccg ggcgcgatag tatccagcgt
    62881 aaagttggaa cagtgcggct taaactgcac acaggtcgac ggcgttgcca gcaggcggcg
    62941 accgttccag tcgagatcca gctcctgatg aatatgaccg cacagcaagt atttgacgtg
    63001 cggaaacttc gccagcacgg tatccagttc gcccgcgtta cgcagactgt gttgatcgag
    63061 ccaactacaa cccgcaggta gcggatgatg atgcagcagc agcaacgtat ggcgttctgg
    63121 cgcatcggcc agtttacgtt ccagccactc aagctgaaac tcgctcagct caccgtgcgg
    63181 cacgccaaac acctggctat ccagcaacag gatttgccat tgctcaccaa taaacacccg
    63241 cttcgccggg gagatacccg catcctgtaa cgcgctgtac atcgcgggct ggaaatcgtg
    63301 gttgcccggc agccagacgc agggcgcacg aaaacttgcg atgccttcag cgaaatgctg
    63361 ataggccgca gaggattgat cctgcgctaa atcacctgtc gcgacaatca ggtcgaattc
    63421 gtgctggtgt ggacgaatcg cctccagcac cgcctggtag ctttcccagg tgtttacccc
    63481 taacagggct tcgtgctttt gtgcaaacag gtgagtatcg gtaatttgta aaatccttcc
    63541 tctggcctca ccagccagag gaagggttaa caggctttcc aaatggtgtc cttaggtttc
    63601 acgacgctag taaaccggaa tcgccatcgc tccatgtgct aaacagtatc gcaaccagtc
    63661 cgctaaaaac tggttaattt gatgcttttc gtcgcgttga tgcaactttt tattaggata
    63721 atcataccgc gctttgaagc gaaaaatctg ctggcttgaa cacacttcag ccaccatcgc
    63781 gtcatgatac agacgcaccg tcattgacgg aaggctccag taactgatcg cgggcgcagt
    63841 ctgttctatt gtcaccaggg tagtgtatcg ggtcgattcc acaatcgtca gccgatattg
    63901 tgcgtttgcc acctgatagc ttacagtttc gccgggtgcg tcattgcgcg gtaacaaacg
    63961 gcgcaattgt gaaaagttca tctcgcacag gcgcatcatt tcaggaaagt caggtgtgta
    64021 acgcttcatt tatgcccact cattttttaa cgcttgatga tgcagctgca gccattgcaa
    64081 agcgatgacc gacgctgcgt tgtcgatttt cccctcttct acccactggt atgcctgttc
    64141 ccggcttacc acatgaacgc gaatatcttc gttttcatca gccagaccat gaataccgct
    64201 tgcggtcgtg gcgtccactt cgcccaccat aattgacgaa cgctcactgg tgccccccgg
    64261 gcttgccagg aaacttaaca ccggtttggt ccgtttgact atcagtcccg cctcttcaat
    64321 cgcttcgcga cgggcaacat cttccacact ttcaccctct tcaatcatcc cggcaaccat
    64381 ctccagtagc cagggggttt cgctggtgtc gtacgcggca atccgaatct gctcaatcag
    64441 cacaacttca tcacgcactg ggtcaaaggg tagcaagact gcggcgtgac cgcgctcaaa
    64501 aatttcccgc cgtacctcat gactcatttg cccgttgaat agacgatgac gaaatctata
    64561 aagatctaat gaaaaaaagc cgcgataaag tgtttctcgt gcaataattt ctacatcgtt
    64621 tttgccaaat gtaacgggca ggttgtctgg cttaagcatt gttaatgtcc tagcactaat
    64681 agtgaattaa atgtgaattt cagcgacgtt tgactgccgt ttgagcagtc atatgttaaa
    64741 ttgaggcaca ttaacgccct atggcacgta acgccaacct tttgcggtag cggcttctgc
    64801 tagaatccgc aataatttta cagtttgatc gcgctaaata ctgcttcacc acaaggaatg
    64861 caaatgaaga aattgctccc cattcttatc ggcctgagcc tttctgggtt cagttcgttg
    64921 agccaggccg agaacctgat gcaagtttat cagcaagcac gccttagtaa cccggaattg
    64981 cgtaagtctg ccgccgatcg tgatgctgcc tttgaaaaaa ttaatgaagc gcgcagtcca
    65041 ttactgccac agctaggttt aggtgcagat tacacctata gcaacggcta ccgcgacgcg
    65101 aacggcatca actcgaacgc gaccagtgcg tccctgcagt taactcaatc catttttgat
    65161 atgtcgaaat ggcgtgcgtt aacgctgcag gaaaaagcag cagggattca ggacgtcaca
    65221 tatcagaccg atcagcaaac cttgatcctc aacaccgcga ccgcttattt caacgtgttg
    65281 aatgctattg acgttctttc ctatacacag gcacaaaaag aagcgatcta ccgtcaatta
    65341 gatcaaacca cccaacgttt taacgtgggc ctggtagcga tcaccgacgt gcagaacgcc
    65401 cgcgcgcagt acgataccgt gctggcgaac gaagtgaccg cacgtaataa ccttgataac
    65461 gcggtagagc agctgcgcca gatcaccggt aactactatc cggaactggc ggcgctgaat
    65521 gtcgaaaact ttaaaaccga caaaccacag ccggttaacg cgctgctgaa agaagccgaa
    65581 aaacgcaacc tgtcgctgtt acaggcacgc ttgagccagg acctggagcg cgagcaaatt
    65641 cgccaggcgc aggatggtca cttaccgact ctggatttaa cggcttctac cgggatttct
    65701 gacacctctt atagcggttc gaaaacccgt ggtgccgctg gtacccagta tgacgatagc
    65761 aatatgggcc agaacaaagt tggcctgagc ttctcgctgc cgatttatca gggcggaatg
    65821 gttaactcgc aggtgaaaca ggcacagtac aactttgttg gtgccagtga gcaactggaa
    65881 agcgcacatc gtagcgtcgt gcaaaccgta cgttcctcct tcaacaacat taatgcttct
    65941 atcagtagta ttaacgccta caaacaagcc gtagtttccg cgcaaagctc attagacgcg
    66001 atggaagcgg gctactcggt cggtacgcgt accattgttg atgtgttgga tgcgaccacc
    66061 acgctgtaca acgccaagca agagctggcg aatgcgcgtt ataactacct gattaatcag
    66121 ctgaatatta agtcagccct gggtacgttg aacgagcagg atttgctggc actgaacaat
    66181 gcgctgagca aaccggtttc cactaatccg gaaaacgttg ccccgcaaac gccggaacag
    66241 aatgctattg ctgatggtta tgcgcctgat agcccggcac ccgtcgttca gcaaacatcc
    66301 gcacgcacta ccaccagtaa cggtcataac cctttccgta actgatgacg acgacggggc
    66361 ttcggccccg tctgaacgta aggcaacgta aagatacggg ttatctgccg cattcttccc
    66421 ccttctcgct tcaatttcga ccagccatcc tctattctga tgggtattta ccactggtcc
    66481 cggaagacaa aaatgaaacg gacaaaatcc atacgccacg catcgttccg caaaaactgg
    66541 agcgcacgcc atctgacacc agtcgctctc gcggttgcca ctgtttttat gctggctggc
    66601 tgtgaaaaga gtgatgaaac agtgtctctc tatcaaaatg ctgacaactg ttcagctgca
    66661 aacccaggca aaagcgccga atgtaccacc gcgtacaaca atgcgctgaa agaagccgaa
    66721 cgtactgcgc cgaaatacgc cacccgtgaa gactgtgttg ctgaatttgg tgaaggtcag
    66781 tgtcagcaag caccagccca ggctggcatg gcaccagaaa accaggcgca ggctcagcaa
    66841 tccagcggga gtttctggat gccgctgatg gccggttaca tgatggggcg tctgatgggc
    66901 ggcggcgcgg gatttgcaca gcagccgctg ttctcctcga aaaacccggc cagtccggct
    66961 tacggtaaat ataccgacgc gacgggtaaa aactatggcg cagcccagcc aggccgcacc
    67021 atgaccgtac cgaagacggc aatggcacca aaaccggcga ccaccactac cgttacccgt
    67081 ggcggttttg gtgaatctgt tgccaaacaa agcactatgc agcgtagcgc aaccggcacc
    67141 tcttctcgtt caatgggtgg ctgataccga tggaaagagt cagtattacc gagcgcccgg
    67201 actggcgtga gaaagcccac gaatacggtt tcaattttca caccatgtac ggcgagccgt
    67261 actggtgtga agatgcttac tacaagttga ccctcgccca ggttgaaaag ctggaagaag
    67321 tcaccgccga actgcaccag atgtgcctga aagtggtgga aaaagtgatc gccagcgatg
    67381 agctgatgac caaattccgc ataccaaaac acacctggag ttttgtgcgt caatcatggc
    67441 tgactcacca gccatcgctt tattcgcgtc ttgatctggc gtgggatggc actggcgaac
    67501 ctaaacttct ggaaaataac gccgatacgc caacgtcact atacgaggcg gcgttctttc
    67561 agtggatctg gctggaagat cagcttaacg ccggtaactt gccggagggc agcgaccagt
    67621 ttaacagtct gcaagaaaaa ctgatcgatc gcttcgttga gctgcgtgaa cagtatggct
    67681 tccagttgct gcatctcacc tgctgtcgcg acacggtgga agatcgcgga accattcagt
    67741 atttgcagga ctgcgcaacc gaagctgaaa tcgccaccga gttcctctac atcgacgaga
    67801 taggtttagg cgaaaaaggc cagttcacgg atttacagga tcaggtgatt tctaacctgt
    67861 tcaagcttta tccgtgggaa tttatgctgc gcgaaatgtt ctccaccaag ctggaagatg
    67921 ctggcgtacg ctggctggaa ccggcgtgga agagcattat ctccaacaag gcacttctac
    67981 cgctactgtg ggagatgttc ccgaatcacc cgaacctgct gcccgcttat tttgcggaag
    68041 atgatcatcc gcaaatggaa aaatatgtgg ttaaaccgat cttctcccgt gaaggcgcaa
    68101 acgtgtcgat cattgagaac ggcaaaacca ttgaagcagc ggaaggtccg tatggcgaag
    68161 aagggatgat tgttcagcaa ttccacccgt taccgaaatt cggcgacagc tatatgctga
    68221 ttggtagctg gctggtgaac gatcaacctg ccggaattgg cattcgtgaa gaccgtgcgt
    68281 tgatcaccca ggatatgtct cggttttatc cacatatttt tgttgaataa gccacgatac
    68341 cggatggcac tcgccatccg gtaattgtta gcctatctgc accgacagca tacttaaact
    68401 gcccatttct ataccatcga ccggaatggt aattggctcc tgcccatccc acacacctaa
    68461 cacatacaac aacggcagat agtgctctgg cgttgggttc gataacgcgc caccttcatg
    68521 gtcgagataa ttcaccagag gatgctgctc taccggccct tgccacgtca gattcgcttt
    68581 cacatactca ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt gccacttcac
    68641 tgtgcgcagg ttatgcacca cgttaccgct ggcgaccaac attattcctt catcacgcag
    68701 cgccgcgagt ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac tgtcgatact
    68761 caactgcacc atagggatat cggcgtcagg atacatctta atcagcacgc cccacgagcc
    68821 gtggtcaaag ccccaggctt ctttatccag cgtcaccggg atcggcgcta acagctcaac
    68881 cagacgctgt gccagcgcag gcgaacccgg agcaggataa tgcgtatcgt acagcgcctg
    68941 cgggaagcca ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca ctcctgttcc
    69001 acgggtaaac cagtgagccg aaaccaccac aatcgcttgc gggcgtggca atgtcatccc
    69061 caacttctgc cagctgcggg tatacaaatt atcttccagc acgttcatcg gactaccgtg
    69121 acctaaaaac aatgctggca tacgtgttga agacatgatg atatccttaa ctaaaggtgt
    69181 cattttgata tcctcacaat acgcttgttc ggcggagtaa gaacccggat aacaatgatg
    69241 atgatcatca gttattttga cgatctgcct gaaggtgaag atttataagg agttgtcgat
    69301 gtcagtacct ctcattctga ccatactggc gggggcagcc acgtttattg gcgcgtttct
    69361 cggcgttctc ggacaaaaac cctcgaaccg cttactggcg ttttcgctag gttttgcggc
    69421 ggggatcatg ttgctcatct cattaatgga aatgcttcct gccgcactag cagctgaagg
    69481 aatgtcgccg gtgttgggtt atggaatgtt tatcttcggt ctgcttggct actttggcct
    69541 ggaccgcatg ttgccgcatg cgcatccgca ggatttaatg caaaaatcgg tgcagctgtt
    69601 gccaaaatcg atcaagcgca cagccattct gctcactctc ggcatcagtc tgcataactt
    69661 cccggaaggg attgccacct ttgtcacggc gagcagcaac ctggagctgg gatttggcat
    69721 cgcactggcc gtcgcgttgc acaatatccc tgaaggtctg gcagtggcag gcccggttta
    69781 tgcggcaacg gggtctaaac gcaccgcgat tctgtgggcg gggatttctg gactagcaga
    69841 aattcttggt ggtgtgctgg cgtggttaat cctcggtagc atgatttccc cggtggtcat
    69901 ggcggcaatc atggcggcgg ttgcaggaat tatggtggcg ctctcggttg atgaattaat
    69961 gccgctcgcc aaagagattg accctaataa taaccccagc tatggcgtac tatgtggaat
    70021 gtcagtgatg ggattcagtt tagtgctgct acaaacggcg ggaattggtt aaaaacaaca
    70081 tcagaagttg tccagcacat ggacaacttc tgccagcttc acgaaagcct tattctacct
    70141 tttcggctgt ctcttctctc gtactgttta agtatttgtt ccgcgcttca agcagtgcgt
    70201 ctctgtcctg gcgaaacgcc cgacaataac gtttcaaatg caggaaataa cccaccacaa
    70261 ccagagcaat caatacaatc cagtaccagg cgataaacat ccttttaccc ttaatattta
    70321 acaatactaa acatttagcg cataaatttc acatatcctt tttcggatat attcatatgg
    70381 tcgcataata acggaacaat tatatgattt tgatcataat gaaaaagaat gcctgatatt
    70441 aatgaaattt tctcatagaa agagaagcgg aattaacatc ttgcatagca gggcaaaata
    70501 aaaaccgaag ccagctacaa cttcggtttc ttgattaagg cagtaattaa gcagcggttt
    70561 tcagctggct ttacgctcat gtgcctgacg gtatgccacc aggtcttcaa tagtcacgag
    70621 cgccatattg tgtttattgg caaactcaat gcactctggt gcacgcgcca tcgtgccatc
    70681 gtcattagtc agctcacaca gcacaccagc cggtttaaag cctgccagcg tcatcaaatc
    70741 aatagtcgct tcagtatgac cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg
    70801 gaaaacgtgg cccgggcgat tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg
    70861 aacggtcgta atacgatcag cggcagaaac accggtagtc acaccttcag ccgcttcaat
    70921 ggtcacggta aaaccggtgc cataggcgct ggtgttattt tctaccatca ttggcagatc
    70981 aagttgttta cggcgatctt cagtgatgca caggcaaaca ataccgctac cgtggcgaat
    71041 ggtcagcgcc atctgctcaa cggtcatggt ttctgccggg aagatcatat cgccttcgtt
    71101 ttcacggtct tcatcatcaa gcaccattac accgcgtcct tcacgcagcg cagccagtgc
    71161 attttcaaca cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa
    71221 aaaacctcac taaaattatg gttaccagaa tcagggcagt cttaggagtg gcggcatata
    71281 gccaaaataa cgtgagcggg tccatgcccg acagaatcgt tactctctcc catccggact
    71341 ctaaccgtcg gccccggaat tacaccggat ctgctgtcct ttgagttcgc acccaaagcg
    71401 ctcgcgggct ttcaactgag ttgatttacc gccggtgggg aatttcgccc cgccctgaga
    71461 ataagcggat tcactataac gctaatgatt agcggcagca acgcatagct tcacataatt
    71521 ctggtttatg acttaccctt atcgcactac aatggtactc aacacctatc agtacaggga
    71581 aactacaatg attgacccga aaaaaattga gcaaatcgct cgccaggttc acgaatcaat
    71641 gcctaaagga atcagggagt tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca
    71701 agctcagctg acgcgcctcg accttgtaag ccgtgaagag ttcgacgtcc aaacgcaagt
    71761 cctgttacgg actcgtgaga aactggcgct gcttgaacaa agaatcagcg aactggaaaa
    71821 tcgcagtact gagatcaaaa agcagccaga tccagagact ctccccccaa cgctgtaaac
    71881 atttgtcttt aatgcactgt aaattatgaa ttaatcctct aatttacagt gttttctatt
    71941 acattatcgc ttaatcccat tcagagtatc atcacaaaaa tgaatgattg caattgaaaa
    72001 tataattgtt atgtattctt taagttttac ttaatcgata
//
LOCUS       DAJTOW010000006        70334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTOW010000006 DAJTOW010000000
VERSION     DAJTOW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.006"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..417
                     /locus_tag="OO244_000457"
     CDS             <1..417
                     /locus_tag="OO244_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5662402.1"
                     /translation="GYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMV
                     AARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQS
                     WTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            428..1519
                     /locus_tag="OO244_000458"
     CDS             428..1519
                     /locus_tag="OO244_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5662403.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1562..2422)
                     /gene="rsmI"
                     /locus_tag="OO244_000459"
     CDS             complement(1562..2422)
                     /gene="rsmI"
                     /locus_tag="OO244_000459"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5662404.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2487..4523
                     /gene="lpoA"
                     /locus_tag="OO244_000460"
     CDS             2487..4523
                     /gene="lpoA"
                     /locus_tag="OO244_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5662405.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4481..4876
                     /locus_tag="OO244_000461"
     CDS             4481..4876
                     /locus_tag="OO244_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5662406.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4896..5486
                     /gene="diaA"
                     /locus_tag="OO244_000462"
     CDS             4896..5486
                     /gene="diaA"
                     /locus_tag="OO244_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5662407.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGYAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5496..6071
                     /gene="yraP"
                     /locus_tag="OO244_000463"
     CDS             5496..6071
                     /gene="yraP"
                     /locus_tag="OO244_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5662408.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6185..7225)
                     /locus_tag="OO244_000464"
     CDS             complement(6185..7225)
                     /locus_tag="OO244_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5662409.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7298..7933)
                     /locus_tag="OO244_000465"
     CDS             complement(7298..7933)
                     /locus_tag="OO244_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5662410.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8061..8579
                     /gene="yhbO"
                     /locus_tag="OO244_000466"
     CDS             8061..8579
                     /gene="yhbO"
                     /locus_tag="OO244_000466"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5662411.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8559..9002)
                     /locus_tag="OO244_000467"
     CDS             complement(8559..9002)
                     /locus_tag="OO244_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5662412.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9053..9355
                     /gene="yhbQ"
                     /locus_tag="OO244_000468"
     CDS             9053..9355
                     /gene="yhbQ"
                     /locus_tag="OO244_000468"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5662413.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9342..9845)
                     /locus_tag="OO244_000469"
     CDS             complement(9342..9845)
                     /locus_tag="OO244_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5662414.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9839..10363)
                     /locus_tag="OO244_000470"
     CDS             complement(9839..10363)
                     /locus_tag="OO244_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5662415.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10572..11567
                     /locus_tag="OO244_000471"
     CDS             10572..11567
                     /locus_tag="OO244_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5662416.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11576..12454
                     /locus_tag="OO244_000472"
     CDS             11576..12454
                     /locus_tag="OO244_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5662417.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12535..13542
                     /locus_tag="OO244_000473"
     CDS             12535..13542
                     /locus_tag="OO244_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5662418.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13660..14904)
                     /gene="mtr"
                     /locus_tag="OO244_000474"
     CDS             complement(13660..14904)
                     /gene="mtr"
                     /locus_tag="OO244_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5662419.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15058..16947)
                     /gene="deaD"
                     /locus_tag="OO244_000475"
     CDS             complement(15058..16947)
                     /gene="deaD"
                     /locus_tag="OO244_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5662420.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16940..17020)
                     /gene="yrbN"
                     /locus_tag="OO244_000476"
     CDS             complement(16940..17020)
                     /gene="yrbN"
                     /locus_tag="OO244_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5662421.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17127..18011)
                     /gene="nlpI"
                     /locus_tag="OO244_000477"
     CDS             complement(17127..18011)
                     /gene="nlpI"
                     /locus_tag="OO244_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5662422.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18120..20255)
                     /gene="pnp"
                     /locus_tag="OO244_000478"
     CDS             complement(18120..20255)
                     /gene="pnp"
                     /locus_tag="OO244_000478"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5662423.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20502..20771)
                     /gene="rpsO"
                     /locus_tag="OO244_000479"
     CDS             complement(20502..20771)
                     /gene="rpsO"
                     /locus_tag="OO244_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5662424.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20920..21864)
                     /gene="truB"
                     /locus_tag="OO244_000480"
     CDS             complement(20920..21864)
                     /gene="truB"
                     /locus_tag="OO244_000480"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5662425.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21864..22265)
                     /gene="rbfA"
                     /locus_tag="OO244_000481"
     CDS             complement(21864..22265)
                     /gene="rbfA"
                     /locus_tag="OO244_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5662426.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22429..25077)
                     /gene="infB"
                     /locus_tag="OO244_000482"
     CDS             complement(22429..25077)
                     /gene="infB"
                     /locus_tag="OO244_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5662427.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREATEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25102..26589)
                     /gene="nusA"
                     /locus_tag="OO244_000483"
     CDS             complement(25102..26589)
                     /gene="nusA"
                     /locus_tag="OO244_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5662428.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26617..27069)
                     /gene="rimP"
                     /locus_tag="OO244_000484"
     CDS             complement(26617..27069)
                     /gene="rimP"
                     /locus_tag="OO244_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5662429.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27276..27352)
                     /locus_tag="OO244_000485"
     tRNA            complement(27276..27352)
                     /locus_tag="OO244_000485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27316..27318),aa:Met,seq:cat)
     gene            27700..29043
                     /gene="argG"
                     /locus_tag="OO244_000486"
     CDS             27700..29043
                     /gene="argG"
                     /locus_tag="OO244_000486"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5662430.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29051..30676)
                     /locus_tag="OO244_000487"
                     /pseudo
     CDS             complement(29051..30676)
                     /locus_tag="OO244_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31135..31221)
                     /locus_tag="OO244_000488"
     tRNA            complement(31135..31221)
                     /locus_tag="OO244_000488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31185..31187),aa:Leu,seq:gag)
     gene            complement(31236..31568)
                     /gene="secG"
                     /locus_tag="OO244_000489"
     CDS             complement(31236..31568)
                     /gene="secG"
                     /locus_tag="OO244_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5662431.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31796..33133)
                     /gene="glmM"
                     /locus_tag="OO244_000490"
     CDS             complement(31796..33133)
                     /gene="glmM"
                     /locus_tag="OO244_000490"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5662432.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33126..33974)
                     /gene="folP"
                     /locus_tag="OO244_000491"
     CDS             complement(33126..33974)
                     /gene="folP"
                     /locus_tag="OO244_000491"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5662433.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34064..35998)
                     /gene="ftsH"
                     /locus_tag="OO244_000492"
     CDS             complement(34064..35998)
                     /gene="ftsH"
                     /locus_tag="OO244_000492"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5662434.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36098..36727)
                     /gene="rlmE"
                     /locus_tag="OO244_000493"
     CDS             complement(36098..36727)
                     /gene="rlmE"
                     /locus_tag="OO244_000493"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5662435.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36853..37146
                     /gene="yhbY"
                     /locus_tag="OO244_000494"
     CDS             36853..37146
                     /gene="yhbY"
                     /locus_tag="OO244_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5662436.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37302..37778)
                     /gene="greA"
                     /locus_tag="OO244_000495"
     CDS             complement(37302..37778)
                     /gene="greA"
                     /locus_tag="OO244_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5662437.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38026..39459
                     /gene="dacB"
                     /locus_tag="OO244_000496"
     CDS             38026..39459
                     /gene="dacB"
                     /locus_tag="OO244_000496"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5662438.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39645..40817)
                     /gene="cgtA"
                     /locus_tag="OO244_000497"
     CDS             complement(39645..40817)
                     /gene="cgtA"
                     /locus_tag="OO244_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5662439.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40833..41798)
                     /locus_tag="OO244_000498"
     CDS             complement(40833..41798)
                     /locus_tag="OO244_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5662440.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41925..42182)
                     /gene="rpmA"
                     /locus_tag="OO244_000499"
     CDS             complement(41925..42182)
                     /gene="rpmA"
                     /locus_tag="OO244_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5662441.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42203..42514)
                     /gene="rplU"
                     /locus_tag="OO244_000500"
     CDS             complement(42203..42514)
                     /gene="rplU"
                     /locus_tag="OO244_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5662442.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42772..43743
                     /gene="ispB"
                     /locus_tag="OO244_000501"
     CDS             42772..43743
                     /gene="ispB"
                     /locus_tag="OO244_000501"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5662443.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43970..44248
                     /gene="sfsB"
                     /locus_tag="OO244_000502"
     CDS             43970..44248
                     /gene="sfsB"
                     /locus_tag="OO244_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5662444.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44296..45555)
                     /gene="murA"
                     /locus_tag="OO244_000503"
     CDS             complement(44296..45555)
                     /gene="murA"
                     /locus_tag="OO244_000503"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5662445.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45610..45864)
                     /gene="ibaG"
                     /locus_tag="OO244_000504"
     CDS             complement(45610..45864)
                     /gene="ibaG"
                     /locus_tag="OO244_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5662446.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46024..46317)
                     /gene="mlaB"
                     /locus_tag="OO244_000505"
     CDS             complement(46024..46317)
                     /gene="mlaB"
                     /locus_tag="OO244_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5662447.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46317..46952)
                     /gene="mlaC"
                     /locus_tag="OO244_000506"
     CDS             complement(46317..46952)
                     /gene="mlaC"
                     /locus_tag="OO244_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5662448.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46971..47522)
                     /gene="mlaD"
                     /locus_tag="OO244_000507"
     CDS             complement(46971..47522)
                     /gene="mlaD"
                     /locus_tag="OO244_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5662449.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47527..48309)
                     /gene="mlaE"
                     /locus_tag="OO244_000508"
     CDS             complement(47527..48309)
                     /gene="mlaE"
                     /locus_tag="OO244_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5662450.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48317..49126)
                     /gene="mlaF"
                     /locus_tag="OO244_000509"
     CDS             complement(48317..49126)
                     /gene="mlaF"
                     /locus_tag="OO244_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5662451.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49336..50301
                     /locus_tag="OO244_000510"
     CDS             49336..50301
                     /locus_tag="OO244_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5662452.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50315..51301
                     /gene="kdsD"
                     /locus_tag="OO244_000511"
     CDS             50315..51301
                     /gene="kdsD"
                     /locus_tag="OO244_000511"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5662453.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51322..51888
                     /gene="kdsC"
                     /locus_tag="OO244_000512"
     CDS             51322..51888
                     /gene="kdsC"
                     /locus_tag="OO244_000512"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5662454.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51885..52460
                     /gene="lptC"
                     /locus_tag="OO244_000513"
     CDS             51885..52460
                     /gene="lptC"
                     /locus_tag="OO244_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5662455.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52429..52986
                     /gene="lptA"
                     /locus_tag="OO244_000514"
     CDS             52429..52986
                     /gene="lptA"
                     /locus_tag="OO244_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5662456.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            52993..53718
                     /gene="lptB"
                     /locus_tag="OO244_000515"
     CDS             52993..53718
                     /gene="lptB"
                     /locus_tag="OO244_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5662457.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53766..55199
                     /gene="rpoN"
                     /locus_tag="OO244_000516"
     CDS             53766..55199
                     /gene="rpoN"
                     /locus_tag="OO244_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5662458.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55222..55509
                     /gene="hpf"
                     /locus_tag="OO244_000517"
     CDS             55222..55509
                     /gene="hpf"
                     /locus_tag="OO244_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5662459.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55627..56118
                     /gene="ptsN"
                     /locus_tag="OO244_000518"
     CDS             55627..56118
                     /gene="ptsN"
                     /locus_tag="OO244_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5662460.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56164..57018
                     /gene="rapZ"
                     /locus_tag="OO244_000519"
     CDS             56164..57018
                     /gene="rapZ"
                     /locus_tag="OO244_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5662461.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57015..57287
                     /gene="npr"
                     /locus_tag="OO244_000520"
     CDS             57015..57287
                     /gene="npr"
                     /locus_tag="OO244_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5662462.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57500..58132
                     /gene="yrbL"
                     /locus_tag="OO244_000521"
     CDS             57500..58132
                     /gene="yrbL"
                     /locus_tag="OO244_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5662463.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58129..58856)
                     /gene="mtgA"
                     /locus_tag="OO244_000522"
                     /pseudo
     CDS             complement(58129..58856)
                     /gene="mtgA"
                     /locus_tag="OO244_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(58853..59506)
                     /gene="elbB"
                     /locus_tag="OO244_000523"
     CDS             complement(58853..59506)
                     /gene="elbB"
                     /locus_tag="OO244_000523"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5662464.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(59736..62072)
                     /gene="arcB"
                     /locus_tag="OO244_000524"
     CDS             complement(59736..62072)
                     /gene="arcB"
                     /locus_tag="OO244_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5662465.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62168..63097)
                     /locus_tag="OO244_000525"
     CDS             complement(62168..63097)
                     /locus_tag="OO244_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5662466.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63772..68232
                     /gene="gltB"
                     /locus_tag="OO244_000526"
     CDS             63772..68232
                     /gene="gltB"
                     /locus_tag="OO244_000526"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5662467.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68245..69663
                     /gene="gltD"
                     /locus_tag="OO244_000527"
     CDS             68245..69663
                     /gene="gltD"
                     /locus_tag="OO244_000527"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5662468.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            69847..70326
                     /locus_tag="OO244_000528"
     CDS             69847..70326
                     /locus_tag="OO244_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5662469.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17220 a        19074 c        17465 g        16575 t
ORIGIN      
        1 ggctatgcag tggttcctta tctcacgact tatcaggaaa accgtctctc ggtagatact
       61 acgcagctgc ccgataacgt cgatcttgaa caaacaacac agtttgtggt gcccaacaga
      121 ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact tgttacagtc
      181 agcgatcgca acggtaaacc gttgcccttt ggcgctcttg ccagcaacga tgatacgggg
      241 caacaaagta tcgtcgatga gggcggcata ctatatctct ctgggatatc gagtaaatca
      301 caaagctgga ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt tgcttttagt
      361 acaccggatt cagaaccaac aacctctgta ttacaaggca cagcgcagtg ccattaagga
      421 taaaaaaatg aaaagagcgc ctcttataac aggacttttg ttgatatcca catcctgcgc
      481 ttatgcctcc tcaggagggt gtggagccga cagcactagc ggtgcgacaa attacagcag
      541 tgtggttgat gatgttacgg tgaaccagac agataacgtg acaggacggg agtttacctc
      601 tgcaacgcta agtagcacta actggcaata cgcctgttcc tgctctgcgg gtaaggcagt
      661 taaacttgtc tatatggtca gccccgtact taccaccact ggacatcaga caggatatta
      721 caaactcaat gacagcctgg atattaaaac cacattacag gcaaacgaca ttccaggact
      781 cacaaccgac caggttgtct ctgttaacac ccgattcaca cagataaaaa gcagcaccgt
      841 atattctgct gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt atggacccgt
      901 taatattggt gcaaatacca cctttaccct gtatgtcacc aagccatttc tcggctcgat
      961 gaccattccg aaaacggata ttgccgtcat taaaggcgcg tgggtcgatg gaatgggaag
     1021 cccgtctaca ggtgacttcc atgatttagt caagttatcg attcagggaa atctcaccgc
     1081 cccacagtcg tgcaaaatta atcagggcga tgttattaaa gttaattttg gattcatcaa
     1141 tggtcagaag tttaccaccc gcaatgccat gccagacggt tttactccag tagactttga
     1201 tatcacttat gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga
     1261 cggtacaact ggggtagtag accagtacaa cctggtcgcc aggcgaagaa gttcagacaa
     1321 tgtgcccgat gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa atattccttt
     1381 tcagaacggt atccttcccg ttgatccttc cgggcatggc accgtcaata tgcgcgcctg
     1441 gccagttaat ctggtcggtg gtgagctgga aacaggaaaa tttcagggca cagccaccat
     1501 taccgtcatg gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg cttcggcact
     1561 gttacccctg ctgctccagc gcatacttat acagcgcatt tttcttcacg ccatgaattt
     1621 ctgcggccag cgccgccgct tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc
     1681 gcagggcatc ggcgggtaag tcttcttcct gtgctttatg accttcgaca atcagcacca
     1741 tttcgccttt gcgacggttt tcatcttcct ttacccacgc cagcagctcg ccaacgggcg
     1801 cgccgtgaat ggtttcccag gttttggtca gctcacgcgc cagaaccacg tagcgggatt
     1861 cgcctaatac cgcaacgata tcttccaggc tatctaacag acggtgggta gattcataaa
     1921 aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt
     1981 tggcaggtaa aaagccttcg taacagaaac ggtcagatgg taaacccgcg gcgcttaacg
     2041 cagtgatagc agcacacggt ccgggtagcg gcaccacacg aatccccgct tcacggcagg
     2101 tacgcaccag atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg
     2161 caatgttttg cccctcttgc agctttgcca gcagcgtttc ggctttttgt tgttcgttat
     2221 ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt
     2281 gacgagtatc ctcggcggca atcagatcaa cggcctgtaa tacctctaac gtacgctggg
     2341 tgatatccgc cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat
     2401 ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc
     2461 gtaaaaaaaa tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg
     2521 cgcgttgtct gcctgttgtt ctggcagccc tgattttcgc cggttgtggc acccatactc
     2581 ccgatcagtc cactgcttat atgcagggca cggcgcaggc tgattctgcc ttttatcttc
     2641 agcagatgca gcaaagctct gatgatacca ggatcaactg gcaattactc gccattcgtg
     2701 cactggtgaa agaaggtaaa accgggcagg cggttgagtt gtttaaccaa ctaccgcaag
     2761 aactgaacga ttctcagcgt cgcgagaaaa cactgctggc ggcagagatt aaactggcgc
     2821 agaaagattt tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc gatttagaac
     2881 aaaaccagca agcgcgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca
     2941 ttgatttact gcgcgcgtta attgctcagg aaccgctgct tggcgcgaaa gaaaaacagc
     3001 agaatattga tgccacctgg caggcgctct cctccatgac tcaggaacag gcgaatacgc
     3061 tggtgatcaa cgccgacgaa aatattctgc aaggctggct ggatctgcag cgcgtctggt
     3121 ttgataaccg taacgatccc gacatgatga aagccgggat cgccgactgg cagaaacgtt
     3181 atccgaacaa tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta
     3241 aaccagcctc gaccaacaaa atcgccctgc tgttgccgct gaatggccag gcagcggtat
     3301 ttggtcgcac tattcagcaa ggctttgaag ccgcgaaaaa tatcggcact cagccagtgg
     3361 cggctcaggt agctgccgca cctgccgcag acgtagcaga acaacctcag ccgcaaactg
     3421 cggatagcgt tgccagcccg gcacaagcct cggttagcga tctgaccggt gatcagcctg
     3481 cggcccagcc ggtgcctgta agcgccccgg cgacaagcac cgcagcggta agcgcacccg
     3541 caaatccatc cgcagagctg aaaatctacg atacctcatc acaaccactt agccagatct
     3601 taagccaggt tcagcaggat ggcgcgagta ttgtggttgg tccgttgctg aaaaataacg
     3661 ttgaagagtt gctgaagagc aacaccccgc tgaacgtact ggcactgaac cagccggaga
     3721 atatcgaaaa ccgcgtcaat atttgttact tcgcgctttc accggaagac gaagcgcgcg
     3781 atgcagcgcg tcatattcgt gaccagggta aacaagcgcc gctggtgctg atcccacgca
     3841 gtgcattggg cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa ctgggcggcg
     3901 gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt gttaacggcg
     3961 gttctggtat tgctttaacg ggtagcccga ttactcccag agcgacaacc gactccggca
     4021 tgacgaccaa caatccaacg ctgcaaacca cgccaaccga tgaccagttc accaataatg
     4081 gcggtcgtgt cgatgcggtc tacattgtgg caacgccggg tgaaatcgct tttatcaaac
     4141 cgatgatcgc catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc agttcccgca
     4201 gcgcacaagg caccgcaggc ccggatttcc gactggagat ggaaggcttg cagtacagcg
     4261 aaatcccgat gctggcaggc ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga
     4321 acaacgatta ttcgctggct cgcatgtatg cgatgggcgt tgatgcctgg tcgttggcaa
     4381 atcatttctc acaaatgcgc caggttcagg gttttgaaat caacggtaat accggaagtc
     4441 tgacggctaa cccggattgc gtgattaaca ggaagttatc atggctacag taccaacaag
     4501 gtcaggtagt ccccgccagt taaccaccaa acagaccggc gatgcgtggg aagcacaagc
     4561 gcgtcgctgg ctggaaggca aaggactgcg gtttatcgcc gctaacgtga acgagcgtgg
     4621 cggcgagatc gatctgataa tgcgtgaagg cctgaccacc gtttttgtcg aggtacgcta
     4681 tcgccgctct gcgctttatg gcggcgcggc agccagtgtg acccgcagca aacaacacaa
     4741 attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg atactgtgga
     4801 ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta aggatgcctt
     4861 taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt aaagcttgct
     4921 tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat gccatctccc
     4981 gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc tgttgtggta
     5041 atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac cgtttcgaaa
     5101 cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc ttaacggcga
     5161 ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg ctgggttatg
     5221 cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt gttaaagcag
     5281 ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt gaccggctat gacggcggcg
     5341 aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg catcgtagtg
     5401 ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg atcgataaca
     5461 cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg ccaatcgcag
     5521 tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg
     5581 ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggtg gacgatggta
     5641 ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag aaagaaaccc
     5701 gtattaatgt cacggcctat cagggcaaag tgctgctggt tgggcagtca ccaaatgctg
     5761 aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac gaagtgtata
     5821 acgagattcg tcagggccag ccgattggtc tgggcgaagc atctaacgat acgtggatca
     5881 ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc aacgtgaaag
     5941 tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt gaagcgaaag
     6001 cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg ggttaccaca gcgtttacgt
     6061 ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct tatcaggcct
     6121 acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca tgttttccct
     6181 caaatcagaa caacagcgcc agcccgccga caatcacacc agacactgcc accatcgccc
     6241 ctgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt gacggcaaac
     6301 tcactgccgg gagcgtcatc aacaatgcca gcgccggagc ggttcccata cctgccagca
     6361 tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag catcctgcta
     6421 ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca tgggggaata
     6481 accagacgcg tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg atcgtactcc
     6541 agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt gcttccggta
     6601 tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt tgcaccagcg
     6661 tcgcaatcag caacaccatc accagcccgg ccaccagacg aatcgccgca aaaccccagc
     6721 cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg cccatccaga
     6781 atgccagcgc accgcccatc gatacctgtt ggcgacgcat tcccgccgcg accggagccg
     6841 cacagcaggt acacatcatg cccggcagcg aaaacagcgt tcccagcagc gtgccgcgaa
     6901 agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc tgaatcaacg
     6961 aaccgaggat cacccccagc accgccgctt tccatactgc gaggaaatag atcatcgcgt
     7021 aatccaacgc cgcttgccac ggattagcat ccgcctgcgc aaggatagat ttaccgatac
     7081 tgtgggtttc ggctgcagta aacgctttgc cgtagtaagg ttgccatttc acataccaga
     7141 gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg ggcgttgccg
     7201 cctgagatga agactgacca gtcatagcat tccccaggaa taagttatga taattgagcg
     7261 cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt ttgcgtaatt
     7321 ctgaagagct taaaatcgtc acgccttcat gctcaggccg catcgcctct gccagcatga
     7381 cccgcgccac gtctctcgca tcaatggatt tccagttacc tggtaacaaa cggaacagcg
     7441 gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagtaac atcgacgggc
     7501 gagcaatggt caatttcggc cagttctggg caattaatgc ttcttccatc tcccctttga
     7561 cgcggttata gaaaaacggc gagtgggcat tggcacccat agcactgacc accagcatat
     7621 gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac taccagcgtg taatcggcat
     7681 gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa caaaacacaa
     7741 tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca tggggattaa
     7801 acaccccggg catatcgccc aacggacgtc gcgtcggcgc agcaatggcg ttaactttcg
     7861 gttcattaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg cctgtaatca
     7921 gtacctgact catcttcgtt cctttacaga attgtctgcc ttgcgctccc cggctcatgc
     7981 accagtctta atagaccatg aggtaattat ccccgattgt ggaaaattcg cctcatccaa
     8041 tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcact gatgaatttg
     8101 aagattcaga atttacttca cccgcagacg agttccgtaa agccggacac gaagtgatta
     8161 ctattgaaaa acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc agcgtgacca
     8221 tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgttg ctaccgggtg
     8281 gtcattcacc ggattatctg cgtggcgaca accgttttgt cacctttacc cgtgattttg
     8341 tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg atcagcgccg
     8401 atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat gttaaaaatg
     8461 cgggcgtgga attttacgat caggaagtcg tggttgataa agatcagctg gttaccagcc
     8521 ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc ggtgcctgag
     8581 tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa tttgatttca
     8641 tcgagccgga tttcccacac cggggccgac agcattctgg caaccggaaa gcgacgattg
     8701 tacgccttgc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc acctttaaac
     8761 tgcacaccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc ggcaaccgca
     8821 gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat gtagaaggcg
     8881 actttctgcg catcaaaaag ataaaaggca ttagcgcacc agagttcccc ttcctgctgc
     8941 acacaccagg tgacaacatg ttgttttgcc agccaacggc tgatggcgat gagtgtttcc
     9001 attgctgttc tcttttatac tgtgggcctg accttaacat gcgatgcaga cgatgacacc
     9061 ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga tcaccacgga
     9121 tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac tgcgcgggaa
     9181 aggagaactg acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg cactacgggc
     9241 ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag cggaaggcgc
     9301 agggtttgca gaactgttga gcagcctgca aaccccggag attaaaagcg attgaaatgc
     9361 tcgtgatact caaccagacc ggttacgcca ttcagcgcgt catccgctaa acgatgtacc
     9421 aggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc cgccagttca
     9481 aagccgaaac gactgtacag cgccggatcg cccagcgtca ccactgcggc atagccgaac
     9541 tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg tccacggtat
     9601 ttttcatcaa ccgccaacgg tgccatgccg acccattgca ggtcttcgcc ctgcacatca
     9661 accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc caccagcccc
     9721 agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc atcactttcg
     9781 aatgagcgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc tactcgaatt
     9841 agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt cccgcctcaa
     9901 caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc tccagttcaa
     9961 tggcgtccat caggtttttc acatacaccc ccagctccgt atcgccttca atcaccagcc
    10021 gacgctggaa gaagagcgta tccggatctt gtttacgcgc cgcaatcatc agcagatcgc
    10081 tggcgtcggc actaaaactc acatcagctt gcgtgttctg gctgaccacc agtttgccat
    10141 tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt aaccagcggc
    10201 cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg gaattgccag cttaagacct
    10261 gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca ctcaacagag
    10321 atggccccaa atgcacaata cgggaacgca gtttatccaa cacgagtttt actccctgtt
    10381 tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa atcaacaatt
    10441 ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca aaaattgtcg
    10501 cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgata gcgcaacctt
    10561 caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt aaggcggcca
    10621 tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgataccaat gcccgtcact
    10681 tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt gtccatcaac
    10741 atcgccgcaa acttcacatc gcgattaaca cttttgcgca tccggacggt tacgcccgtt
    10801 ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc ctcgccgacc
    10861 tcgccatgct ggagtacgcc gccgagcgtt acccgcatat tgaacgtcat gtgtcagtgc
    10921 aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt gacgttgccc
    10981 gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca cgggttactc
    11041 ctgtcccgct ggaagtgttt gctttcggca gcctgtgtat tatgtcggaa ggccgttgct
    11101 atctgtcgtc gtatctgacg ggtgagtcgc ccaacactgt gggtgcgtgt tctccggccc
    11161 gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac gaagtgctga
    11221 tcgaccgtta tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa gggcgttatc
    11281 tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat accctggaac
    11341 tgctgccgga gttaatggct gcgaatattg cttcggtgaa aattgaaggc cgccagcgta
    11401 gcccggcgta tgtcagccag gtggcgaaag tctggcgtca ggctatcgac cgttgtaagg
    11461 ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg tcgatgtccg
    11521 aaggcacgca aaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa gagcaatgaa
    11581 atattcctta gggccagtgc tgtggtactg gccaaaagag acgctggaag atttttatca
    11641 gcaagccgcc gccagcagcg ccgacgtgat ttatcttggt gaagcggtat gcagcaagcg
    11701 ccgggcaacc aaagttggcg actgtctgga gatggcaaaa tcgctcgccg gaagtggcaa
    11761 gcagattgtg ctctccacgc tggcgctggt gcaggcatcc tctgaactgg gcgaactgaa
    11821 acgctatgtt gagaacggtg aatttctgat tgaagccagc gatctcggcg tggtgaatat
    11881 gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct acaacgcggt
    11941 gacgctgaaa atattgctca aacagggcat gatgcgctgg tgtatgccgg tggagctttc
    12001 ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc gcaaccagtt
    12061 tgaagtggaa gtcctgagct acggtcatct accgctggcc tactccgccc gctgctttac
    12121 cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta agtatccgaa
    12181 cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg gcattcagac
    12241 catgagcggc tacgtttaca acctcggtaa cgagctggca tccatgcagg gattggttga
    12301 tgtagtccgc ctgtcaccgc agggcactga cactttcgcg atgctcgacg ccttccgcgc
    12361 taatgaaaat ggcgctgtgc cactgccgtt gacggcaaac agcgactgta acggctactg
    12421 gcggcggctg gcaggactgg agctgcaagc ctaagtaaat agctcacttt gttaacaact
    12481 ttaactattc tttaatgcag tattaaagat taatcggtaa caaagtgagc tgttatgact
    12541 gataaaacca ttgcgttttc gctactcgat ctggccccca tacccgaagg ctcttcagcg
    12601 cgggaagcgt tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat
    12661 cgctactggc tggcagaaca ccacaatatg accggcattg ccagtgctgc aacatcggta
    12721 ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg cggcgtgatg
    12781 ttgcctaacc acttaccgtt ggtcattgcc gaacagttcg gcacactcaa tacgctctat
    12841 ccggggcgaa tcgatttggg gctgggtcgt gctccgggta gtgaccaacg gacaatgatg
    12901 gcgctacgtc gtcatatgag cggcgatatt gataatttcc cccgcgatgt cgcggagctg
    12961 gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc aggctatggc
    13021 gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gtgcacaact ggcggcgcag
    13081 cttggtctgc catttgcgtt tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg
    13141 catctttatc gcagtaactt caaaccgtcg gctcggctgg aaaaaccata cgcgatggtg
    13201 tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt tacctcaatg
    13261 cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc gccgattcaa
    13321 aatatggatc agttctggtc accgtctgag cagtatgacg tgcagcaggc gctgagtatg
    13381 tcgttggtag gcgataaagc gaaagtgcgt catggtttgc agtcgatcct gcgcgaaacc
    13441 gacgctgatg agattatggt taacgggcag attttcgacc accaggcgcg cctgcattcg
    13501 tttgagctgg cgatggatgt taaggaagag ttgttgggat agtgtgtctt aacgcgggaa
    13561 gccttatccg agctggcaac gctgtcctac gtaggcctga taagcgaagc gcatcaggca
    13621 ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca ccggcagtaa
    13681 attaaagctc gataaaatat gcaccagtgc gttgccgacg ccaaacacca gaatcagcgc
    13741 aatcatcggc ttgccacccc agacgcggaa tttcgggctg ccaaagcgtt tacgcgatgc
    13801 gcgggccagc agtgccggaa caattgccgc ccagatggta gccgctaaac cagcataacc
    13861 aatggcatac aggaatccgt tcgggaacag cagaccaccc acaacaggcg gggcaaaggt
    13921 cagcaatgcc gttttcaagc ggcccacagc cgagtcgtcg aaaccaaaca gatctgccag
    13981 atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa agtttgagaa
    14041 cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg cttgtaccaa
    14101 cacatcaata ttaccgccct tctcggcgat gccgataaac tccggacgag ggatgttgcc
    14161 cattgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac cgtacaccag
    14221 gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggcttg gcacgttacc
    14281 gtgataacca aacgacgcca ggcagaacgg cagggtcatc agcaaatacg gcgcataaga
    14341 tgcattgctt tcggcaacgt tgaacaatgt cgcaggctgc acatgcccca gcaggctacc
    14401 gaaggtgagg aagaaggtaa tgactttcgc ccccagcaca atcgccgtca tgcggctgac
    14461 ggctttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa aacccgccgc
    14521 ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaatcg aaccactggc
    14581 agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatgc cgttgaccac
    14641 gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac cgattcggta
    14701 attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc aggtaaagat
    14761 cagcgctgcc attgaccaga aaaaccacgc cccggacatg accactggca gagaaaacat
    14821 ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg acggtgacgt
    14881 ttgggtggtg gttagtgttg ccatgagggc ttctctccag tgaaaaatag tgcgactgcg
    14941 ttgttatgca ttgcactgta ccagtacatg agtacaaaag acagaaaaaa agccccgatg
    15001 gttaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca gcgatgatta
    15061 cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggtg cgcggccttc
    15121 acgacgttcg ccgctaaaac gacgaccatc accacgacca ccttcacggc gttcaccgct
    15181 gaagttacga ccgccttcac gacgctcgcc accgaaacca cgaccaccgc cacgacgctc
    15241 accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct tgttgagaat
    15301 gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca gttcgatggt
    15361 ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg ctgctgatgt cgccttcgtt
    15421 agcaatcgcg ccaacgatat gacgaacttc aacaccatca tcgcggccca cttcaatgcg
    15481 gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt cacggtcgcc
    15541 acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac ggaattcacg
    15601 tttcggacgc atcggcgcat ctggcggaac aatcagagta cgttcaccct gtgccatttt
    15661 cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag tcggctgaat
    15721 tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct gctgtacttt
    15781 agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca gttctacttc
    15841 cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct cgcggttctc
    15901 aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga tacggtgaac
    15961 gtaagactca gaatccatcg ggatatcgta gttaaccacc aggctgatac gctcaacgtc
    16021 caggccacgg gccgcaacgt cggtcgcaat caggatgtcc agacgaccgt ctttaaggcg
    16081 ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg ttcagcgcgg cgctattgta
    16141 gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac gaacgaagat
    16201 aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt ttttacgcat
    16261 accccacaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc tggactgaat
    16321 acgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg cttccggcat
    16381 ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa tggtttcaac
    16441 gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca gaccgctcag
    16501 tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg gagtaccgac
    16561 aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct ggccgccgta
    16621 cagggcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg cttcagcaac
    16681 ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt tcagctcagg
    16741 atcaagattc tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc ccgtctgggc
    16801 catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct gaattggaga
    16861 tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca ggcccagatc
    16921 tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta atggcggcca
    16981 gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt gaaccggctc
    17041 aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg caatcaaaaa
    17101 agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag gtcatcttgg
    17161 tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa gttatgaacg
    17221 ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc cagacttagg
    17281 tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga ggtgttatcc
    17341 gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc caggtagaac
    17401 tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg tttcaatact
    17461 tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag actacggaaa
    17521 ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa cttgtcacga
    17581 ccgccgtaat ataatgcaat cccgcgattc aagtgcgcgt agttgtaagt tggatcaagc
    17641 tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg cgttaaatat
    17701 atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc ttgcgaaaaa
    17761 tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg ctcatataaa
    17821 agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc catacgtgcc
    17881 agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc acttttacgc
    17941 caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca ccagcgcaaa
    18001 aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt tcccggttgc
    18061 taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa atggcaacct
    18121 tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga ctgctcagtc
    18181 gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttccagaac tttcaccggt
    18241 acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc agcgatttga
    18301 gagatgtgta ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc aaagtcaacg
    18361 atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt gatctcttcg
    18421 atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcaatctt cacagtaccg
    18481 tcatcttcga tttcgatggt ggtgccagtt tcttcggtca gggcacggat aacagagccg
    18541 cctttaccga taacgtcttt gatcttgtcc gggttgatct tgatggtatg gatacgtggt
    18601 gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac gcccaggata
    18661 tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt ggtgatacct
    18721 tcaattttga tatccatctg caatgcagag ataccctcgc gggaacccgc aactttgaag
    18781 tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac gtagttgtcg
    18841 ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgatcgg cacacctgcg
    18901 tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaggaacc gttggattca
    18961 gtgatttcag acacaacacg tacggtgtac gggaatttgt ccatatccgg catgactgcc
    19021 agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg agaaccgacc
    19081 atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa ggtatcggta
    19141 cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cagtacccag cgttgcggta
    19201 accagcgcct gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg cggcagcacg
    19261 ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat acgcggttcg
    19321 cctgccagta cgcggctacg aacaacgttt ttctcgatcg cgtgcagaat ttcacccagt
    19381 tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc agatttgatg
    19441 acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc gctcaggcga
    19501 gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc cggctgccag
    19561 tcccaacgtg gtttaccggc ttctttcacc agttcattga tgttctgaat aacaacctgc
    19621 tgttgttcat gaccgaacac tactgcgccc agcatctggt cttcgctcag cagttcagct
    19681 tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc cagtttgctc
    19741 tctttcaact cgtcctgagt cgggttcagt acgtactggt cattgatgta acctacgcga
    19801 gcagcaccaa tcggaccatt gaacggaata ccagacagag acagtgctgc ggaagcacca
    19861 atcatcgcga cgatatccgg gttaacttgc gggttaacag aaaccacggt ggcgataacc
    19921 tgaacttcgt tgacgaagcc ttccgggaac agcggccgaa tcgggcggtc aatcagacgc
    19981 gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac ggaagaagct acccgggata
    20041 cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa gtcctgacct
    20101 ggtttggctt ttttctggcc aacaacggta acaaataccg cggtgtcatc catgctaacc
    20161 ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac ggtgtgttgg
    20221 ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct ctttcttaga
    20281 cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc gactaatgac
    20341 aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac ctctgcaacg
    20401 gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt cagcttgaaa
    20461 aaaggggcca ctcaggcccc cttttctgaa actcgcaaga attagcgacg cagacccagg
    20521 cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc gagcagttta
    20581 cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt tttgtgctct
    20641 gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg aacttcagta
    20701 gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt tgcttcagta
    20761 cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat ctctaattca
    20821 gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta agcggcagta
    20881 ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt attcaaccac
    20941 caggcgacga ggcgcaacgc ggccttcatc gtcaatttcg cccataccga taaatttgcc
    21001 gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg tacgaactgg
    21061 gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg ggtagtccga
    21121 agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg gaatatcctg
    21181 ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcacaatcc gttcaaccgg
    21241 atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac cgagtttttc
    21301 acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa tttccagctc
    21361 cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg ggcgcgcttc
    21421 acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct gatatttcag
    21481 tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca gcgccgccgc
    21541 cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt cggcatcaga
    21601 agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg agtccagcag
    21661 atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgccag ttgccagcgg
    21721 gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca ctttttgcag
    21781 cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt taatgtcgcg
    21841 accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt aacacgacgt
    21901 tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc ttcaaccaga
    21961 gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc tttccccagc
    22021 aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt aaccgcgtct
    22081 tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc gcgagacatt
    22141 tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc acgctgcagg
    22201 atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc aaattctttc
    22261 gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa attaatttca
    22321 ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc aggcaatcca
    22381 tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt aagcgatggt
    22441 acgttggatc tcgatgattt cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta
    22501 gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt catctttgaa
    22561 gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca gaacgcggat
    22621 cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg caccaaattt
    22681 cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt tcagttccgg
    22741 agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga tgacggagta
    22801 gtaacgcaga tccaggcttt ccgcttcaat cactttacgt gcagaggcat cagcacgtac
    22861 gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg tttcggtgat
    22921 accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca gtttcagcaa
    22981 ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga tgttcacttc
    23041 gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt tctgctgacg
    23101 cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac gcgctttctt
    23161 ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca ggccgaggat
    23221 ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt tacgcatcgc
    23281 acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca gagtaccttc
    23341 acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt cgataaccgc
    23401 accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct gcagcaggat
    23461 agcgtccagc agttcatcga taccagtacc cgctttcgca gacacatgta cgaactggct
    23521 ttcaccgccc cactcttccg gcaggatgcc gtactgggag agttcgttct taacgcgatc
    23581 cggatcggct tctggtttat cgatcttgtt cactgcaacg accaccggaa cctgcgccgc
    23641 tttcgcgtgc tggattgctt cgatcgtctg cggcattacg ccgtcgtcgg cagcaacaac
    23701 caggactacg atgtccgttg cctgcgcacc acgagcacgc attgaagtaa acgcggcgtg
    23761 ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat
    23821 gtgctgggta atgccgcccg cttcgccaga ggccactttc gttgaacgaa tgtagtccag
    23881 cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg cgcgcggttc
    23941 agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt tttcacgacg
    24001 caggataact ttatggccca tctcttcagc aaccagctgt gcggtttcct gatcgataac
    24061 ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct gagagccttt
    24121 aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca caacgtcacg
    24181 gttaacggcc tgagcaggct tctggaagcc ttgctgcagc gaagaacctt tacgttttcc
    24241 gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg
    24301 tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac ggccgccttc
    24361 gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag tgacgtgata
    24421 atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt ccgccatacg
    24481 acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc
    24541 agctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag cctgggcgtt
    24601 tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt cacgtttcgc
    24661 ttgttcagca gcttcacgct tagcttgttc tgtggcctca cgttcagctt tttgttgcgc
    24721 ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctg caaggcgttc
    24781 agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga
    24841 tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt
    24901 caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg
    24961 tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag
    25021 gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct
    25081 gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc
    25141 cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat
    25201 gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa
    25261 atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct
    25321 ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc
    25381 aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag
    25441 ttcttccagc gtcgagaaac cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc
    25501 gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag
    25561 gtcgtcaacg gtcattacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt
    25621 ctgaccgtta cggccaatcg cctgcgccag gttaccagct tcaacggcga tatccatggt
    25681 gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac
    25741 aaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc ccagttcagt
    25801 agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg
    25861 tttatcgttg gttttcaccg cgattttcgc acgagaaccc gggtcgcgag ccgctgcttt
    25921 aatttcaatc acttcttcgc cgatttccgg cacttcaata cggaacagtt cgatcagcat
    25981 ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata
    26041 aagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg
    26101 caggatcacg gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt
    26161 tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc
    26221 acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg tggtgatacg
    26281 gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaagttca ggctttcatc
    26341 ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac
    26401 taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cacggacgtc
    26461 gatctcttgc tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa
    26521 aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc
    26581 tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc
    26641 cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat
    26701 ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg
    26761 gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg
    26821 gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac
    26881 atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc
    26941 ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgcctac
    27001 cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa
    27061 tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt
    27121 cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac
    27181 tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct
    27241 tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc
    27301 gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac
    27361 gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg
    27421 agacgggacg taaaatctgc aggcattata gtgatccatg ccacattttg tcaacgttta
    27481 ttgctaatca cgtgaatgaa tatccagttc actttcattt gttgaatact tttgccttcc
    27541 cctgctctcc cttaagcgca ttattttaca aaaaacacac taaactcttc ctgtctccga
    27601 taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga gagcggaaat
    27661 ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat tctcaagcat
    27721 ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac cagtgccgca
    27781 ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa cctgggccag
    27841 ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac
    27901 gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc tattcagtgt
    27961 ggcgcatttc ataacaccac cggcggcctg acctatttca acacgacgcc gctgggccgc
    28021 gccgtgactg gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa tatctggggt
    28081 gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttatgg tctgctgacc
    28141 aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga tgaactgggc
    28201 ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa aatgtctgtc
    28261 gaaaaagcct actccacaga ctccaacatg cttggtgcaa cgcatgaagc gaaggatctg
    28321 gaatacctca actccagcgt caaaatcgtc aacccgatta tgggcgtgaa attctgggat
    28381 gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg tcatccggtg
    28441 gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc taaccgcatc
    28501 ggcggtcgtc acggcctggg catgagcgac cagattgaga accgtatcat cgaagcgaaa
    28561 agccgtggta tttacgaagc tccggggatg gcactgctgc acattgcgta tgaacgtctg
    28621 ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg tcgtcagttg
    28681 ggccgtctgc tgtaccaggg gcgttggttt gactcccagg cgctgatgct gcgtgactct
    28741 ctgcaacgct gggttgccag ccagatcact ggtgaagtta ccctggagct gcgccgtggg
    28801 aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc agagcgtctg
    28861 acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtattggtca attgaccatg
    28921 cgtaatctgg atatcactga tacccgagag aaactttttg gttatgccaa aactggcctg
    28981 ctttcctcct ctgccacttc aggcctgccg caggtggaga atatggaaaa caaaggccag
    29041 taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgcttatcc tctgtaaact
    29101 cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc cgtgagtctc
    29161 ctttatagtg tgcaatcact tcctcaagcg tttgcggctg ttccggcttt gttaccccca
    29221 tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa gatattgttt tccgttgtgc
    29281 gatccacgcc atccccaaga acggggctat accagataaa catcgggaca tgatatgcct
    29341 gctggctagc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg tcacgttcca
    29401 ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttaataat tcaaaaacct
    29461 gacctagcaa actatctgtg taatgaatgg agttgtcata gcaggcatcc tgatcgtcct
    29521 gcggttgaaa cacggcgctg gattgcggat aggcgctaca agccggttca tggcttccat
    29581 ttaatgaaga acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg
    29641 cggcaacaac aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat
    29701 actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg
    29761 aaatcctgct tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact
    29821 caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct
    29881 tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga
    29941 gcgtgtatat ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac
    30001 gtaggtatca atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat
    30061 tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg
    30121 cgtataggta gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc
    30181 atctttataa gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa
    30241 tattcctgtc actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa
    30301 aagaagggat aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac
    30361 aacttcatcc ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc
    30421 aaaggtacac catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg
    30481 tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag
    30541 gacgaagaac agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat
    30601 accaaaaaca ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt
    30661 tgttgaatac tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat
    30721 gttcaatttt atatcagaca tgtttttcaa ctctgatcca tatcagaccg aaacactgtt
    30781 agttttttct ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa
    30841 tgcgtctcta aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat
    30901 ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag
    30961 tgccaattat gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt
    31021 caaggtacca gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat
    31081 cagaccagga ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac
    31141 cgaggacggg acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc
    31201 taccaattcc accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc
    31261 ttagccggag cagctggctg agtttgttcg gttttcgccg gtgcactcag attttcccat
    31321 tcgctacctc tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag
    31381 aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca
    31441 aacagcgtag cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc
    31501 tgcagcatga tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct
    31561 tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa
    31621 gcgggagttt ttaatctttc ccactgaagc gggtgtgaat actaaccaaa acgaatgacc
    31681 ttcgcaaggg caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt
    31741 gttcgccgag aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa
    31801 cggcttttac tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc
    31861 cttccaccat cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac
    31921 ggttgcccag cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg
    31981 gatcgccgct acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc
    32041 cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc
    32101 cagcaacgat gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct
    32161 ctgcaccgat acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt
    32221 tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca
    32281 tcaatgtacc cacagcacca ccacgcagct ggccctgacg aagaccttca cgcgcgatga
    32341 tatacatgat ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat
    32401 cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga
    32461 gcgcgcgaac gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac
    32521 cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg
    32581 caccgtttgc acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg
    32641 tggctttgca aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca
    32701 gttcagccga atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta
    32761 ccgcatccgg cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg
    32821 ggttatgcga tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag
    32881 ccacggccgg tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac
    32941 ccgcttccag tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa
    33001 tcttacggga gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa
    33061 aatcaggtgt gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt
    33121 tacgattact catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc
    33181 gcttctacgg tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca
    33241 atgaccgcac aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc
    33301 ccaatcatcg attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca
    33361 gccaggcgcg ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg
    33421 tcgagcaaca atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata
    33481 aagtagcgat tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt
    33541 ggatttccct gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga
    33601 gcgccaggtt cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac
    33661 tcacggatga cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca
    33721 attgcctcaa ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc
    33781 cctgggcgcg tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga
    33841 ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca
    33901 ggcgtgacgt tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg
    33961 gcaaagagtt tcattatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg
    34021 ccccggggtt ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact
    34081 gctctgacat ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct
    34141 ttggactacc attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg
    34201 gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt
    34261 tcatgagagc atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg
    34321 cacgattata gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt
    34381 catcggacat atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt
    34441 ccgcgtacag cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg
    34501 ccaggttggt cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt
    34561 agatgatctc ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct
    34621 gacggctggc gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac
    34681 cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa
    34741 tcgcatgacc cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg
    34801 agcgacgttc cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca
    34861 caacgcgttt gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt
    34921 cagcaccgga gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca
    34981 atggtacgcg acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc
    35041 cgaccacaac ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg
    35101 gacggttagt cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa
    35161 ccagcatctg gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac
    35221 gctggcggcc tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg
    35281 cctgttcgaa catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt
    35341 cagaaccgga gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca
    35401 acgttttacc ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga
    35461 gtttctggaa gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg
    35521 cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc
    35581 gcgctttgct cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca
    35641 tgaagaagat ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag
    35701 ccagcaggct tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt
    35761 tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct
    35821 tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct
    35881 cttgtaggaa ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct
    35941 ggaatactga catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt
    36001 cactcaaggg attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata
    36061 tccagcatct ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta
    36121 caatatacac ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg
    36181 taaacaggga gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca
    36241 ctacaaaact gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca
    36301 gatacatggc tcgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg
    36361 acatgacaac ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt
    36421 catcacgaaa atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat
    36481 cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac
    36541 ccggagcagc accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct
    36601 gtatttcatc aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa
    36661 catatttatc gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac
    36721 ctgtcattta actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc
    36781 gcctatttgg tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa
    36841 gcaaaaaata cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat
    36901 ccgctcaagc cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag
    36961 attgaacaag cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc
    37021 gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag
    37081 gtcatcggta aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca
    37141 cgctaagatt atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg
    37201 caggagagca aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc
    37261 tttctttaca atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta
    37321 attacttcaa attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct
    37381 ttgccgatca ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca
    37441 gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc
    37501 gttacggtag caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc
    37561 tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt
    37621 tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg
    37681 atagcagcaa tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat
    37741 ttttcagcgc cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc
    37801 ctgatggggc aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc
    37861 gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca
    37921 ttttgatggt cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt
    37981 gcgccgtagt atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga
    38041 tttatcatcg gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag
    38101 tacgttactc aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg
    38161 tcggcccccg ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa
    38221 gtgattactg cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg
    38281 cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt
    38341 ggtgccgatc cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa
    38401 tctggcgtca accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac
    38461 gataaagccc ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct
    38521 gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt
    38581 gatatggctt ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc
    38641 accctccccc gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaagcgac
    38701 ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct
    38761 tttgccgtgc aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag
    38821 gcgggtatca cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg
    38881 gtagttgcca gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag
    38941 tcggacaaca tgatcgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg
    39001 cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt
    39061 gtcgatattg gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt
    39121 gcccccgcca ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac
    39181 tttatctcca tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg
    39241 catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat
    39301 aacctggcgg gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt
    39361 tctggctatg cagttaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt
    39421 gaaagccgtt tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca
    39481 tggccggggt ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac
    39541 gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg
    39601 ataagtgtag cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg
    39661 aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct
    39721 tcagcaatct cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct
    39781 ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc
    39841 cagcagagat ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcttcc
    39901 cagcccagcg cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga
    39961 tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc
    40021 agctcgctga taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata
    40081 tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga
    40141 cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt
    40201 tcgttgtcca ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc
    40261 actttcggtt tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc
    40321 cccaacatac cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc
    40381 ggcgtaccgt tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg
    40441 cccagaccgt gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca
    40501 cccatggttt caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc
    40561 acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca
    40621 cggaaagatt tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc
    40681 catacgtcac caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt
    40741 tcgcggcgga agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct
    40801 tcatcaacaa acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc
    40861 accgttgtat gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg
    40921 cccgcaacca cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag
    40981 aaatcgggcc aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc
    41041 gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca
    41101 tatcctacca aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc
    41161 tgcgctatca ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt
    41221 aaaaacagga tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa
    41281 ctcacccaaa ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc
    41341 tttgtaaata tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc
    41401 aatgccccta caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc
    41461 atcatgccaa ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc
    41521 aaggatgagc tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa
    41581 atcaaccagc gtggcttacg aaacacgcgt aatggcggca acctcttctt caccgcaaga
    41641 atggcaccaa ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc
    41701 atcacctcca gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta
    41761 agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca
    41821 tttgcggatt aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg
    41881 caacgtgttg cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg
    41941 ctgataaatt tacggttttt cgggccttta acttcgaatt tcactttacc gtctgcttta
    42001 gcaaacagag tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca
    42061 cgttgacgaa cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc
    42121 aagcgtttag cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc
    42181 atttgaaatc tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc
    42241 actgacgatg gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa
    42301 ctttctcgcc acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag
    42361 gaacgccgat tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag
    42421 tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt
    42481 gttgtttacc accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct
    42541 tttcaatgat tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac
    42601 gcacgccttt gacaagtgct acagtcaata cacgaagaaa aaaacacaac ttgtacggta
    42661 acgtttatct gtgccatttt ttcagtacaa ttaccctata ttcctaacca taaaccctaa
    42721 gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta
    42781 gaaaaaatca atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag
    42841 cagcttaatt ccgacgtcca actgatcaat cagttaggct attacatcgt cagcggcggc
    42901 ggtaaacgta ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga
    42961 aatgcgcatg tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctacac
    43021 gacgacgttg tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgccgcattt
    43081 ggcaatgccg ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg
    43141 accagcctcg gttcactcaa agtgctggaa gtcatgtcag aagctgtaaa cgtcatcgca
    43201 gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac
    43261 atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg
    43321 attctggctg gctgtacgcc ggaggaggag aaaggcctgc aggattatgg gcgctatctc
    43381 ggcactgctt tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta
    43441 ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg
    43501 atgcatcatg gcacaccaga acaggcacag atgatccgta ccgccatcga acaaggtaac
    43561 ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg
    43621 acgcgtcagc gcgccgagga agaagcagac aaagccatcg cagcgttaca ggtgctcccg
    43681 gacacccctt ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt
    43741 taatcccctc ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac
    43801 actaattcag cgctataaca cctgaaaaaa gggcgatgaa tctttatata ttctgaatat
    43861 tcacactctt tacaggaact ttttagcgca atagaccatc aggagtatag tgatgcccga
    43921 cagaagaagt gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa
    43981 tttcattgac tggcatcccg ctgacatcat tgcaggtttg cgcaagaaag gaacgtcaat
    44041 ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg cattatcgcg
    44101 cccatggccg aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat
    44161 ctggccatca cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat
    44221 gcgtagctac actaaaccga aaaaatgagt ggtctggcgg tagccccgcg aacggggctg
    44281 ccagctctca gacgattact cgcctttcac acgctcaata tttgcaccta aagcgcgcag
    44341 tttgtcttca atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt
    44401 cccttccgca atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat
    44461 aacctgtgca ccagaaagtt tttcaacacc atgacaaata acggtattgc tttcgatttc
    44521 ggcgtgcgcg cccatacggc tcagctctgg cacatgcata aagcggtttt caaagaccgt
    44581 ttcggtgatg aacccggtcc cttcagccac caggttcaac agcgtgaact gggcctgcat
    44641 atcggtcggg aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc
    44701 atgcatatcc aggctaatcc agtcttcgcc gacttcgatg tccgctccag catcacgcag
    44761 tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta cggcagataa ttttgccacg
    44821 agagatcgcc gccgccacca ggaaagtacc ggtttcgata cgatccggca gcacgcgata
    44881 gacaccgccg cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct
    44941 aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc
    45001 tgcgttttca ataatcgtgg tgccttccgc cagggttgca gcacacatga tggtcaccgt
    45061 tgcgccaacg ctgactttat ccatcacgat atgtgcgcct ttcagacgac catcgacgga
    45121 agctttaacg taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat
    45181 gtgtagatca accggacgcg caccgatcgt acaaccgcca ggtagtgaaa cttgcccctg
    45241 accaaagcgc gctaccagcg gccccagcgc ccagatagaa gcacgcatgg ttttaaccag
    45301 atcgtaaggt gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg
    45361 ttctactttc gcacccagct ggcttagcag tttcattgat gtatcgacgt ctttcagttt
    45421 cgggacgttc tggatctcta ccggttcttc cgccagtagt gcggcaaaaa gaataggcag
    45481 agcagcattt ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac
    45541 acgaaattta tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc
    45601 gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc
    45661 tttgatcgac acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac
    45721 cgtctgctgt tttttaaccc gactcatacc gtcaaacaac tcacccacgg caataacctg
    45781 aaagtggctg ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac
    45841 gctctgaatt tcattatttt ccatgggatc ttcaatcatc agttaataaa ccagcgaaac
    45901 atcttagagc aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca
    45961 aggctcgact tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa
    46021 aatttaacga ggcagaacat cagcaggcaa attatacaat ttcgcgaggg tatacacttt
    46081 gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat cgataagatg
    46141 gagcagcagt gccagtcccc ccgtatccac gcgggacaca cggctaagat cgatgcaggt
    46201 aatccccttc accgcctcct cacgcatttc ccaaagcggt agcagaacgt cctgatccag
    46261 ctctccggat aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat
    46321 tttttctctt ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg
    46381 tcgatacctt tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg
    46441 acgccttcag caatcatgtc gtaagcttgc cagtttcccg tctgggagtt tttacgccac
    46501 tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa taatggtaac gcgaataggc
    46561 acaatggttt tatcgcccag cggctgttct ggcgcaatct gataggtttg accgtgatac
    46621 atcgccagcg cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaggct
    46681 tcacgttgag caggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat
    46741 ttcacctgta cgtatggcag cagttcctga tcaacaatgg tacgcagata atccgggttg
    46801 gcccgaattt gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc
    46861 atcagcttat aaggattggt ctggtctgcc gcggttgccg cactcagagg tgcaatcacc
    46921 agcaaagcga ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt
    46981 gtacccacag gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta
    47041 ttcttattgt catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc
    47101 atcgcagact tggtgtcctg aattgtatcg ccatccttca ggatagcagt ccccagttcc
    47161 gggtcttcaa aaccgacgtt taatgccaga tattgttctc ccagcaggcc ggaagtacga
    47221 atgctcagcg aactggtatc aggaatgtgg ttataacgtt gttcaatttc cagcgttacg
    47281 cgcggcagat aggttttcgg gtccagcgta atatccgcca cccggcccac aacaacacca
    47341 ccaatgctga ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc
    47401 gtgtaggtcg attcagtacg tatggacgtc acgttcgccg ccttcaggca aacaaacagc
    47461 gccgccagca gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc
    47521 atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag
    47581 agacgagtgg acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggc
    47641 gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat
    47701 cagacagttg accagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa
    47761 cccgctatca atgcctttcc aactgacgcc gaccagcgat ccgccccaga tccccacggc
    47821 gacgaagata accgtcaaca gtggtaatga aataacccca gcccagaaac ggggagaaat
    47881 aacccgacgc agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg
    47941 catcaggccg atttctgcgg ttaacgccga accagcacgc ccggcaaaca acagcgcggc
    48001 aaccaccggc cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc
    48061 cgcgctataa gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac
    48121 gccagaaact acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag
    48181 cagcggcgca tgtttgcgaa attccggttt gccgaccagc gcattgaata acattaaccc
    48241 ggcccgcccg aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt
    48301 taacagcatg agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat
    48361 agcggaacgg aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag
    48421 gattcgtctg caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc
    48481 aggcgtgatc cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca
    48541 cgcccagcgc gctgttcagc tcagaaatca gcttcaccag tacgcccatg gtgatgggat
    48601 cttgcccaac aaaaggttca tcaaacatga tgagatccgg ctccagcgca atcgcacgcg
    48661 ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga aggcattagt ttagccgctc
    48721 cacgcagccc cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggaa
    48781 gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca
    48841 acgccccgga ctgaaataac atgctcatcc gtttacgcac tgtatacagg cgcgaacgag
    48901 acatcgccgg aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc
    48961 cgccaatcag acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga
    49021 tcttccctcg cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa
    49081 aactgacatc gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt
    49141 cttgcgttga ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga
    49201 atattacccg ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt
    49261 caaaattaag aatccgttac ggctttctga aaatcttcag cggaccggcg agtatacctg
    49321 aagaaaggac gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc
    49381 gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc
    49441 ccgccgctga tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc
    49501 gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac agccctcggc
    49561 tcaaacatta tcaatatatt gctgatcctc ggtctggctg cgctggttcg tccttttacc
    49621 gtccattccg atgttctgcg ccgtgaatta cccttaatgt tgttggtcag cgtggtggcc
    49681 ggttccgtac tctatgacgg acaacttagt cgaagcgatg gtatctttct cctctttctg
    49741 gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc aggggactga cagcctgacc
    49801 agagagcagc ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc
    49861 ggcattgcgc ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg
    49921 ctggcgaatt actttgccat cagcgagttg acgatggctc tgacggcaat tgctatcgga
    49981 accagtctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt
    50041 gctgtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc
    50101 gcgctgataa cgccaggaga gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg
    50161 ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt
    50221 ggtgtagggg tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta
    50281 tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg
    50341 ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga
    50401 gcttgatcaa tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa
    50461 agggaaagtt gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc
    50521 aacgtttgcc agcaccggta caccttcatt tttcgtccat cctagtgaag ccgcgcatgg
    50581 tgatttaggc atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc
    50641 cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat
    50701 caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt
    50761 agcgaaagaa gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt
    50821 tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt
    50881 tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat
    50941 tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt
    51001 gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat
    51061 tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg
    51121 tcagttaagt attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct
    51181 ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga
    51241 tggcgaccat ttactcggtg tgttacatat gcatgattta ctgcgtgcag gcgtagtgta
    51301 aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc
    51361 cctgtcagcg ccgacgttat ggcaaaagca gagaacattc gtctgctgat cctcgacgtt
    51421 gatggcgtac tgtcagatgg cctgatttat atgggcaata acggcgaaga gctgaaagcg
    51481 ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct
    51541 atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact
    51601 cacttgtatc aggggcagtc aaacaaactg atcgccttta gcgatttgct ggaaaaattg
    51661 gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc tcatcgactg gccggtaatg
    51721 gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc
    51781 gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta
    51841 ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac
    51901 gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt aatatggccg
    51961 aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc
    52021 atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac
    52081 acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt
    52141 ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg
    52201 accggatgct ctatttatat ggacacgttg aagtcaacgc acttgtgcca gactctcaac
    52261 ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg
    52321 aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca
    52381 acttacgcag caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa
    52441 acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg
    52501 gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct
    52561 cttgatatgc aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc
    52621 atcaaaatta atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa
    52681 gtgattgacg gatacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc
    52741 gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg
    52801 ggtaatgctt atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg
    52861 gtgaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg
    52921 gtgccgtcgc agctgcagga caaaaacaac aaaggccaga ccccggcaca gaagaagggt
    52981 aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt
    53041 cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg
    53101 ccaaacggtg ccggtaagac caccactttc tacatggttg taggcattgt gccgcgcgat
    53161 gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg
    53221 cgccgcggta tcggctatct gccacaggaa gcctccattt tccgtcgcct cagcgtttac
    53281 gataacctga tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac
    53341 cgcgcgaacg agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag
    53401 tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg
    53461 aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt
    53521 aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac
    53581 gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc
    53641 gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg
    53701 gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc
    53761 tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc
    53821 tccaacaggc aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc
    53881 aggcgctgga gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc
    53941 gcgaaacgca agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc
    54001 cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg
    54061 gcaccagcgg tgactacatt gacgacgagc tgccggtcta ccagggcgaa acgacgcaga
    54121 ccttgcagga ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg
    54181 ctattgctac ctctatcgtc gatgccgttg atgaaaccgg ttatctgact gtcccgctgg
    54241 aagatattct cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc
    54301 ttaagcggat ccaacggttt gacccggtcg gtgtagcggc aaaagatctg cgtgactgcc
    54361 tgctaatcca actctcccaa ttcgataaga ccacgccgtg gctggaagag gccagattga
    54421 tcattagcga tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca
    54481 cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc
    54541 gccccgggca gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc
    54601 gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa
    54661 tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta
    54721 tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata
    54781 cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg
    54841 aagaatatat gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat
    54901 cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac
    54961 tgaagtattt cttttccagt cacgtcaata ccgagggcgg cggcgaagct tcctccacgg
    55021 cgattcgtgc actggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg
    55081 acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg
    55141 cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac
    55201 ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc
    55261 gaggcactgc gcgaatttgt tacagctaaa tttgccaaac ttgagcaata ttttgaccga
    55321 atcaaccagg tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca
    55381 ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc
    55441 attgatggct taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa
    55501 caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg
    55561 ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt
    55621 gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt
    55681 acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg
    55741 gcggcgaaac aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa
    55801 aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa acttgaagaa
    55861 gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc
    55921 atcgacaacc aaccggtgga cttacttttt gccctgctgg tgccggcaga ccaaactaaa
    55981 acgcacctgc atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc
    56041 cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt
    56101 actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag
    56161 tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt
    56221 gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat
    56281 ctggctcgaa ctctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat
    56341 atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca
    56401 ccgcaactac tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg
    56461 cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa
    56521 agcgatttgc tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg
    56581 tccgttcacg agctggctga aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa
    56641 ctgaccatgg tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac
    56701 gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca
    56761 ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc
    56821 taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc
    56881 tacctgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag
    56941 caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg
    57001 gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc
    57061 atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt
    57121 tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt tattgcgctg ctgatgttgg
    57181 attctgccaa aggacggcag attgaagttg aagcgaccgg tccacaggaa gaggaagcac
    57241 tggccgccgt tatcgccctc tttaattctg gttttgatga agattaatct tcatcacttt
    57301 gacatacaac tcccttcaag ctcccccgac aataagaaaa tcacgtactg aaatcgttct
    57361 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    57421 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    57481 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    57541 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    57601 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    57661 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    57721 cggaaccggt tatgtctacg atgtgattgc cgattttgac ggcaagcctt ccattacgct
    57781 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    57841 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    57901 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    57961 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    58021 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    58081 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    58141 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    58201 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    58261 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    58321 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    58381 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag
    58441 cccttttcgt acccagctac gcccatccca taaaaagaga ttttggccgt ctgctgagaa
    58501 atcgttgaag caccgcgaat acggttttca ttgcgctcgt tgtgcgccag ggctttctca
    58561 atggaagcga catcaaagcc ccagtgctca ggaaatttct gatcttctgc ggcaatcacc
    58621 gccagtccca tccacggcga gatttgatcc atactgaccc agtcagaatg tgccacgtaa
    58681 cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga ccattaccgc tgagaaggga
    58741 acaggcgcaa cgctaaacaa cgcgatgccc ccgccccaga aaacggcgag gacaaccatt
    58801 aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta agcggctttt actcattcag
    58861 ccagaaccag cacgcgggaa accagcttat caatgccgct cgccgcttct gcaatgttct
    58921 gcgccagcat atatgctggg gtggtgacaa tcttattgtc ttcatcaacc acgatatcat
    58981 cgacaggaca cggcacatgc tccgcgccca tctcttccag cacttctgcg gtatcgatat
    59041 cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt cggcagcatc gccggggcaa
    59101 tacacataaa accaagcggt tttccggcct gatgcatcgc ttgtgccagc gcctttaatt
    59161 cacggtcaac ggtgcattcg ctaccaagac tggcaaaatt gcttaaattc ttcgccgcgc
    59221 caaacccccc cggcacaatc aacgcatcca gttcagcggc atcggcctgg gccagaggac
    59281 ggatttcacc gcgcgttatt cgtgccgctt caatcagcac attgcgcgtt tccgtcatcg
    59341 cttcgccagt taaatggttg ataacatcaa cctgctgctt atccggtgca aagcagaccg
    59401 cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac cgcttcatga atttcagaac
    59461 cgtcatagac gccgcatccg ctcagaatta cgccaatttt cttcattgtg atcatccttt
    59521 cgcaattgac tgaaacacat taattttttt aataaaaatg gtacgcatca cacatttaac
    59581 tgattcatgt aacaaatcat ttaagttttg ctatcttaac tgcgtgcggc ctgaaaaaca
    59641 gtgctgtgcc cttgtaactc atcataataa tttacggcgc agccaagatt tccctggtgt
    59701 tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt ttttagtggc ttttgccacc
    59761 cacgctttca gcacttctac gtcgtgacgc cactcttctt tcatctcttc aatccattca
    59821 ccgacgttat cttcccaggc gggaaggtca ggagactgaa tttgctgacc cagctgttgc
    59881 agatggcgta accccactga ccccgccgca cctttaattt tatgtccttc ctcaacaatg
    59941 ccttttttat cctgcgccgt cagattcgac tccagcacgc tgacatagcc cggcatcatc
    60001 ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc ctacaagttc gagatactgt
    60061 tccagcatgg gaatatcgag caatgcttct gatttactgt tctcttctgt cgtcaccgta
    60121 ctctcctcat catcctgggt atcccagaat ttcttgatca tcgcggttag cgccggaacc
    60181 gaaagcggct tactcagcac atcatccatt ccagcattga ggtactcttg tttgtctttc
    60241 agcacgttag cggttaaggc caccagcggc ggtaaatcct cgcgcggata acgcttcgtc
    60301 agttcacgag agatatccag cccggtcata tctggcaact gaatatccag caacaccagg
    60361 tcgtattcgc ccggtttaaa catctccagc gccgccttgc cggtcatggc gacatcaacg
    60421 ctgttaccta atttttccag cacagaacgc gcgacaatca cgttcagttc aatgtcttcc
    60481 accagcagca cattcagcgc cggtaaaggc atatcgtctt catcaaacgc atcatcgacc
    60541 tcttctgcca ccgacggtgc gtggatcgtc aacgtaaagg ttgaaccttt gccctgttca
    60601 ctggtaaccg taatatcgcc gcccatattt ttcgccagac gacgagaaac ggccagacca
    60661 ataccggtgc cggtggcagg tttaccgcca tgactgtctt tcacctggta atacatggcg
    60721 aagattttat ccagctcatc ctgcggaatg ccgataccag aatcttccac ttcaaaatgc
    60781 agcatatcgc cttcatcgta gcgcacgcgc acggtaacct ggccttgctg ggtgaatttg
    60841 acggcgttac tgatgaggtt ccacaggatc tgccgtaaac gcgtcccgtc ggtaatgacc
    60901 tgatgcggta atggcagcgt aggctccagg ttaaagcgca atcctttttg ttgcgcctgc
    60961 aatgcggaga gattttccag atcggcaagg aagctggtga aatcaaccgg ctgattatca
    61021 agctggacct tgcgccgttc catcttatcc atgtcgataa tatcgttaaa gatattcccc
    61081 agcgtgacgg ccgaaacatg gatagttttg agatattttt cctgctcggc ggtgagttcg
    61141 gtatccagca gaatgcggct caggccgacg ataccgttca gcggtgtacg caattcgtga
    61201 ctgatggtgg aaataaacgt cgttttgtcg cggctggcgc gttcaagcgc atcctgatac
    61261 cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac cgtgacgttt acccacgcgg
    61321 tcgtagtacg gcactttacg gatttcaaag caggctttgc gcccgtccgg gtaatccagc
    61381 cactgttcat aggtcagtga cacattatga cggaacactt tttcatcggt ttcaatgact
    61441 tttgcggcgg cttccggtga gtaaacatca gcaggtttca ggtgaaccag ttgtttttcg
    61501 ctttttccgg tcagcagctc catcgcgcgg ttacagccgg aaaactcttt atcttcgtta
    61561 cgataaaaaa ccaagtcggg tgaagcatca aggaaggaac gtaagaatga ggattgctgc
    61621 tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa ttttcagttg gccgaaggtt
    61681 tcctgcagtt ctgcttccgc tttttcacga acggcaattt cctgatttag ctgggcaata
    61741 ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc gcatctcctc cagtttttgc
    61801 accagccgtg acagacgttg tcgtgactcc tccagttgct cgacgaccac cgatagaaag
    61861 tagaccgccc acggcgtaat cagcaaacca aagaagatag aacgaataac atcaatgctt
    61921 tcgacctgac catgcagcac catggttacc gccatttgta ccacaatggc aagaacgacg
    61981 agggccagcg ccagcaacat tgagaagcgc accagaccta acttcatcat caggtcaaca
    62041 taatactgcg ccagcagacg aatttgcttc attagggaat tccttcacga caacctgccc
    62101 caataatacc caattctgac agttacgtta taaattgtgt gagaaatgcg gaattcccct
    62161 caacgaccta ctccgttgga ggtagccacg gacgtcccag cgccgatccc tgtacgccat
    62221 gttcattcag atagcgatcc agctcgacca ttcccgtcca tcgattttcg caccacagcg
    62281 gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg gtggtagatc acttccggtg
    62341 gcgtatggcg aatcatttct ccggcagtga gcgtataatc ctccagttca ataccgttca
    62401 aacgccccgc ttcccaggct ttcgccataa tgctgccttt cacaatatgc agcggatgca
    62461 gctttatgcc gtccacgctg gtttcaacca ctcgctccag cgtttgcaaa cattcagcct
    62521 gcccttcgcc aggcaggccg acaattaagt gggaacacac cttcagcccg cgctgacgtg
    62581 ccagctgggt tgtacgctga taacaggcaa aatcatggcc gcggttgatg cgatgcagtg
    62641 ttttgtcgtg ggcggtttgt agccccagct ccagccacac ttcgtaaccc tggtccttat
    62701 attcgcaaag cagatccagc accgcatccg gcacgcagtc cgggcgggta ccaacacaca
    62761 aaccgacaat attggcctgg ctcaccgcct gctgatacat agaacgcagc acctgaactt
    62821 ccgcaaaggt gctggtatac gcctgaaagt aggccagata gcgtttagcg cggttaacta
    62881 aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg ctgctgcgct tcatcggcaa
    62941 acgaggcaac attacagaat gtgcagccac cacgcccgat ggtaccgtca cggttagggc
    63001 agctaaaacc gccgtgcagc gtcagcttat gcaccttttg cccataacga cgggtgagat
    63061 caccaccaaa catattgact aatttctgta actgcataat ctgatagacc gcgccttgaa
    63121 aagaggccaa agcctgccat ttttagctca attcggcgat gacctggatc aatcgtcccg
    63181 gcctgctttt atcaactaca taatcaatca aaattaccga aatttcatgc acaatcacat
    63241 aaatcacttt tgcttatctt gtgtcagatt tttttatctc cttatggatt ttaggcaaaa
    63301 acagtagcat gaaacgtcat taccaattaa ggcagtataa aatgctggtt ttctcgtcag
    63361 ttcaaggcag gataagggtt aacacgcctt tatgacagtc aggaattgac tgtttctcta
    63421 acgactctcc ttttagcctt aaagataaaa tctattttaa tttcagctat ttaataaaga
    63481 attttgcgct aaagcacatt tctgtaccaa taagcttgcc atttgacctg tatcagcttt
    63541 cccgataagt tggaaatccg ctggaagctt tctggatgag cagcctgctc atcatattta
    63601 tgcagtaatt gagatcccct cttcaccgta ttaaccgatg cgaaaaggac aacaaggggg
    63661 cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc acgctctttc tgtgcccgtg
    63721 cgcagcggtt cggaagtggg gttcccgcag agcctggggg aggttcacga tatgttgtac
    63781 gataaatccc ttgagaggga taactgtggt ttcggcctga tcgcccacat agaaggcgaa
    63841 cctagccaca aggtagtgcg tactgcaata cacgcactgg cccgcatgca gcaccgtggc
    63901 gcgattctcg ccgatggtaa aaccggcgac ggttgcggct tgctgttaca aaaaccggat
    63961 cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt tagcaaaaaa ctacgctgtc
    64021 gggatgctct tcctgaataa agatcctgaa ctcgccgctg ccgcacgccg catcgttgaa
    64081 gaagaactgc aacgcgaaac cttgtcgatt gtgggctggc gtgatgtccc cactaacgaa
    64141 ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca ttgagcaaat ttttgtgaac
    64201 gccccggcag gctggcgtcc acgcgatatg gagcgccgtc tgtttatcgc ccgccgccgc
    64261 attgaaaagc gtctcgaagc cgacaaagac ttctacgtct gtagcctgtc gaatctggtg
    64321 aacatctata aaggtctgtg tatgccggcg gatctgccgc gcttttatct ggatcttgcg
    64381 gacctgcgtc tggaatcggc catttgcctg ttccaccagc gcttctccac taacaccgta
    64441 ccgcgctggc cgctggcgca accgttccgc tatctggcgc ataacggtga aatcaacacc
    64501 atcaccggta accgccaatg ggcgcgtgcg cgtacctata aattccagac gccgctgatc
    64561 cctgacctgc acgacgccgc gccattcgtc aacgaaaccg gttctgactc cagttccatg
    64621 gataacatgc tggaactgct gctggcaggc gggatggata tcatccgcgc catgcgcctg
    64681 ttagtgccac ccgcctggca gaacaacccg gatatggacc cggaactgcg tgccttcttt
    64741 gactttaact ccatgcatat ggagccgtgg gatggtccgg cgggcatcgt gatgtccgat
    64801 ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc gtccggcacg ctacgtcatt
    64861 accaaagaca agctcatcac ctgcgcctct gaagtcggta tctgggatta ccagcctgac
    64921 gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga tggtgatcga cactcgcagt
    64981 gggcgtattc tgcactcggc agaaaccgat gacgatctga aaagccgcca cccttataaa
    65041 gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg aagatctgcc cgatgaagaa
    65101 gtgggtagcc gcgaactgga cgacgacacg cttgccagct accagaaaca gtttaactac
    65161 agcgcggaag agctggactc cgtaattcgc gtactgggcg aaaacggtca ggaagcggtc
    65221 ggttcgatgg gcgatgatac cccattcgcc gtgctctcca gtcagccgcg cattatttac
    65281 gactacttcc gccagcagtt tgcccaggtg actaacccgc caatcgaccc gctgcgtgaa
    65341 gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa tgaacgtctt ttgcgaagca
    65401 gagggccagg cgcaccgttt aagctttaaa tcgccgattc tgctctactc cgatttcaaa
    65461 cagctcacga cgatgaaaga ggagcactac cgcgcagata cgctggatat cacctttgac
    65521 gtcactaaaa ccacgctcga agcgacagtc aaagagctat gcgacaaagc cgaaaaaatg
    65581 gtacgtagcg gcaccgtgtt gctggtgctc tccgaccgga atatcgctaa agatcgcctg
    65641 ccggttccgg ctccgatggc ggttggcgcg atccagaccc gtctggtcga tcaaagcctg
    65701 cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc gcgatccgca ccacttcgcc
    65761 gtgttgctgg gtttcggcgc gacggctatt tatccgtacc ttgcctatga aacgctgggc
    65821 cgcctggtag acacccacgc gattgccaaa gattatcgta ccgtgatgct caactaccgt
    65881 aacggcatca acaaaggctt gtacaaaatc atgtccaaaa tgggcatctc caccatcgcc
    65941 tcttaccgct gctcgaaact gtttgaagcg gtcggtctac acgatgatgt agtgggcctg
    66001 tgcttccagg gcgcggtcag ccgcattggt ggggcaagct ttgaagactt ccagcaggat
    66061 ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc cgattagcca gggcggcctg
    66121 ctgaaatacg tccacggcgg cgaataccac gcctacaacc cggacgtggt gcgcacgctg
    66181 caacaggcgg tacaaagcgg cgagtacagc gactatcagg aatacgcgaa gctggttaat
    66241 gagcgtccgg caaccacgct gcgcgatctg ctggcaatta cgccgggtga aaacgcggtc
    66301 aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac gctttgatac cgccgcgatg
    66361 tctatcggcg cgttaagccc ggaagcccac gaggcgctgg cggaagcgat gaatagcatc
    66421 ggcggtaact cgaactccgg tgaaggcggc gaagacccgg cgcgctatgg cactaacaaa
    66481 gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg ttactccggc gtatctggtc
    66541 aatgccgacg tcattcagat taaagtcgcc cagggcgcga agccgggcga aggtggtcag
    66601 ttgccgggcg acaaagtcac tccttacatc gccaaactgc gctattcggt gcccggagtg
    66661 acgctgatct ccccgccgcc gcaccacgat atctactcta tcgaagactt agcgcagctt
    66721 attttcgacc tcaagcaggt taacccgaaa gcgatgatct ccgttaagct ggtgtccgaa
    66781 ccaggcgttg gcaccatcgc gactggcgtg gcgaaagctt atgcggactt gatcaccatc
    66841 gcgggctatg acggcggcac cggcgcaagc ccgctgtcgt cagtgaaata tgcaggctgc
    66901 ccgtgggaac tcggtctggt ggaaacccaa caggcgctgg ttgccaacgg cttgcgtcat
    66961 aaaattcgtt tgcaggttga tggcggcctg aaaacgggtg tcgatatcat caaagcggcg
    67021 attctcggcg cagaaagctt cggcttcggc actggcccaa tggtggcgct cggctgtaaa
    67081 tatctgcgta tttgccacct gaacaactgc gccacgggtg tagcaactca ggatgacaaa
    67141 ctgcgtaaga atcactatca cggcctgccg ttcaaggtga ctaattactt tgagtttatc
    67201 gcccgtgaaa cccgcgagct gatggcacag ctgggagtaa cgcgtctggt ggatctgatt
    67261 ggtcgcaccg acctgctgaa agagctggac ggtttcaccg ccaaacagca gaaactggcg
    67321 ctgtcgaagc tgctggagac tgccgagcca catgcaggta agtcactcta ctgcactgaa
    67381 aacaacccgc cgtttgataa cggcctactg aacgcgcagt tgctgcaaca ggcgaaaccg
    67441 tttgtcgatg aacgccagag caaaaccttc tggttcgata tccgcaacac cgaccgttct
    67501 gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg gcgatcaggg gctggcagcc
    67561 gatcctatca aagcgtactt caacggcacc gcaggccaga gtttcggtgt gtggaacgcg
    67621 ggcggcgtgg aactgtacct gaccggggat gccaacgact atgtcggtaa aggcatggcg
    67681 ggcggcttaa tcgccattcg tcctccggtt ggttccgcct tccgcagcca tgaagcaagc
    67741 attatcggca acacctgcct gtatggcgcg accggtggtc gtctgtatgc cgcaggccgc
    67801 gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca ccgtggtaga aggcattggc
    67861 gacaacggct gtgaatatat gacgggcggt attgtctgta ttctgggtaa aaccggcgtt
    67921 aacttcggcg cgggcatgac cggcggcttc gcttacgttc tcgatgaaag cggtgatttc
    67981 cgcaaacgcg ttaacccgga actggtcgaa gtcttaagcg tcgacgatct ggcgatccat
    68041 gaagagcatc tgcgcggtct tatcaccgag catgttcagc ataccggttc tcagcgcggt
    68101 gaagagattc tggcgaactg gtcaaccttc gccacgaaat tcgcgctggt taaaccgaag
    68161 tccagtgatg taaaagcact gctgggtcac cgtagtcgta gcgcagcaga gttgcgcgtg
    68221 caggcgcagt aaggggtagc aacaatgagt cagaatgttt atcaatttat cgacctgcag
    68281 cgcgttgatc cgccaaagaa accgctgaag atccgtaaaa ttgagtttgt tgaaatttac
    68341 gagccgtttt ccgaaggcca ggccaaagcg caggctgacc gctgtctgtc gtgcggcaac
    68401 ccatactgcg agtggaaatg cccggtacac aactacatcc caaactggct gaagctcgcc
    68461 aacgaggggc gtatttttga agcggcagaa ctgtcgcatc agaccaacac actgccggaa
    68521 gtatgcggac gtgtctgccc gcaagaccgt ctgtgcgaag gttcctgcac cctgaacgat
    68581 gagttcggtg cggtgaccat cggcaacatt gagcgttata tcaatgataa agcgttcgaa
    68641 atgggctggc gtccggatat gtccggcgtg aagcaaaccg gtaaaaaagt ggcgattatc
    68701 ggtgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga cgcgtaacgg cgtaaaagcc
    68761 gttgtcttcg accgtcatcc agaaattggc gggctgctga ccttcggtat tccggccttc
    68821 aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct tcaccggcat gggtattgaa
    68881 ttcaaactca ataccgaagt gggccgcgac gtacagctgg acgatctgct gagtgattac
    68941 gatgccgtgt tccttggcgt tgggacttat cagtcaatgc gcggcgggct ggaaaacgaa
    69001 gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg ccaacaccaa acagttaatg
    69061 ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg aaggcaaacg cgtggtagta
    69121 cttggcggtg gcgacactgc gatggactgc gtgcgcacgt ctgtacgcca gggggcaaag
    69181 cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc cgggttcccg ccgcgaagtg
    69241 aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg tccagccact ggggattgaa
    69301 gtgaacggta acggcaaagt cagcggcgta aaaatggtac gtaccgaaat gggcgaaccg
    69361 gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt ccgaacatat cgttccggca
    69421 gatgcggtga tcatggcgtt tggtttccgt ccacacaaca tggaatggct ggcaaaacac
    69481 agcgtcgagc tggattcaca aggccgcatc atcgctccgg aaggcaacga caacgctttc
    69541 cagaccagca acccgaaaat ctttgctggc ggcgatatcg tccgtggttc cgatctggtc
    69601 gttaccgcca tcgccgaagg tcgcaaagcg gcagacggca ttatgaactg gctggaagtt
    69661 taagctctgt cccagcgccg cctcctcaga aaagagtcgg cgctgaaatt ctcctttctt
    69721 tctggaaatc gcctcgcata atctgaagaa aaccaatgta atacgctcat ctggattgtt
    69781 accggtagat gcccggcgta tagctgattc cattattcaa cgcagcgcca tataaaggga
    69841 cagataatgg aatcgctctc agagagaacc tcaacaggct accagcaaat ccacaacggc
    69901 attattcatc tggtcgatag cgcccgaacg gaaacggtac gtagcgttaa cgcgctgatg
    69961 accgcgacgt actgggaaat tggccgacgg attgtcgaat ttgaacaagg tggtgaggcc
    70021 agagcagctt atggtgcaca gttgattgat cggctatcga aggatttaag tcgacggtat
    70081 aaacgtgggg tctctgcaaa aaacttacgc caaatgaggc ttttttacct cttttttcaa
    70141 catgttgaaa ttcgccagac agtgtctggc gaattaacac cattgggaat tccgcagaca
    70201 ccatctgcag aattcccatc ggcaaagatt tggcagacgc tgtctgccaa attgagaggc
    70261 tgtttgatat ttccacagct tttgtattgc atctcctatt ttcggtttgt atattcatgc
    70321 aaataaacga agga
//
LOCUS       DAJTOW010000007        67659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTOW010000007 DAJTOW010000000
VERSION     DAJTOW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.007"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..229
                     /locus_tag="OO244_000529"
     CDS             <1..229
                     /locus_tag="OO244_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5662470.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="OO244_000530"
                     /pseudo
     CDS             624..1263
                     /locus_tag="OO244_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="OO244_000531"
     CDS             1366..1689
                     /locus_tag="OO244_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5662471.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO244_000532"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO244_000532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5662472.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMHSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO244_000533"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO244_000533"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5662473.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO244_000534"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO244_000534"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5662474.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO244_000535"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO244_000535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5662475.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO244_000536"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO244_000536"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5662476.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO244_000537"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO244_000537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5662477.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO244_000538"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO244_000538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5662478.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO244_000539"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO244_000539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5662479.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO244_000540"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO244_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5662480.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO244_000541"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO244_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5662481.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO244_000542"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO244_000542"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5662482.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO244_000543"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO244_000543"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5662483.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO244_000544"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO244_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5662484.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO244_000545"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO244_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5662485.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19264..20481
                     /gene="alaA"
                     /locus_tag="OO244_000546"
     CDS             19264..20481
                     /gene="alaA"
                     /locus_tag="OO244_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5662486.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20565..21164
                     /gene="yfbR"
                     /locus_tag="OO244_000547"
     CDS             20565..21164
                     /gene="yfbR"
                     /locus_tag="OO244_000547"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5662487.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21223..23055)
                     /locus_tag="OO244_000548"
     CDS             complement(21223..23055)
                     /locus_tag="OO244_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5662488.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23142..23792)
                     /gene="hxpA"
                     /locus_tag="OO244_000549"
     CDS             complement(23142..23792)
                     /gene="hxpA"
                     /locus_tag="OO244_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5662489.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23803..24297)
                     /locus_tag="OO244_000550"
     CDS             complement(23803..24297)
                     /locus_tag="OO244_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5662490.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24380..24835)
                     /locus_tag="OO244_000551"
     CDS             complement(24380..24835)
                     /locus_tag="OO244_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5662491.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25173..26375
                     /gene="ackA"
                     /locus_tag="OO244_000552"
     CDS             25173..26375
                     /gene="ackA"
                     /locus_tag="OO244_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5662492.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26450..28594
                     /gene="pta"
                     /locus_tag="OO244_000553"
     CDS             26450..28594
                     /gene="pta"
                     /locus_tag="OO244_000553"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5662493.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28784..30304
                     /gene="yfcC"
                     /locus_tag="OO244_000554"
     CDS             28784..30304
                     /gene="yfcC"
                     /locus_tag="OO244_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5662494.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30337..30879)
                     /gene="yfcD"
                     /locus_tag="OO244_000555"
     CDS             complement(30337..30879)
                     /gene="yfcD"
                     /locus_tag="OO244_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5662495.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30937..31488)
                     /gene="yfcE"
                     /locus_tag="OO244_000556"
     CDS             complement(30937..31488)
                     /gene="yfcE"
                     /locus_tag="OO244_000556"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5662496.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31544..32188)
                     /gene="yfcF"
                     /locus_tag="OO244_000557"
     CDS             complement(31544..32188)
                     /gene="yfcF"
                     /locus_tag="OO244_000557"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5662497.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32324..32971
                     /gene="yfcG"
                     /locus_tag="OO244_000558"
     CDS             32324..32971
                     /gene="yfcG"
                     /locus_tag="OO244_000558"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5662498.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33028..33390
                     /gene="folX"
                     /locus_tag="OO244_000559"
     CDS             33028..33390
                     /gene="folX"
                     /locus_tag="OO244_000559"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5662499.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33411..34304
                     /locus_tag="OO244_000560"
     CDS             33411..34304
                     /locus_tag="OO244_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5662500.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34352..35242)
                     /gene="rpnB"
                     /locus_tag="OO244_000561"
     CDS             complement(34352..35242)
                     /gene="rpnB"
                     /locus_tag="OO244_000561"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5662501.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35439..36212)
                     /gene="hisP"
                     /locus_tag="OO244_000562"
     CDS             complement(35439..36212)
                     /gene="hisP"
                     /locus_tag="OO244_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5662502.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36220..36936)
                     /locus_tag="OO244_000563"
     CDS             complement(36220..36936)
                     /locus_tag="OO244_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5662503.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36933..37619)
                     /gene="hisQ"
                     /locus_tag="OO244_000564"
     CDS             complement(36933..37619)
                     /gene="hisQ"
                     /locus_tag="OO244_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5662504.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37709..38491)
                     /gene="hisJ"
                     /locus_tag="OO244_000565"
     CDS             complement(37709..38491)
                     /gene="hisJ"
                     /locus_tag="OO244_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5662505.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38712..39494)
                     /gene="argT"
                     /locus_tag="OO244_000566"
     CDS             complement(38712..39494)
                     /gene="argT"
                     /locus_tag="OO244_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5662506.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39760..40329)
                     /gene="ubiX"
                     /locus_tag="OO244_000567"
     CDS             complement(39760..40329)
                     /gene="ubiX"
                     /locus_tag="OO244_000567"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5662507.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40424..41941)
                     /gene="purF"
                     /locus_tag="OO244_000568"
     CDS             complement(40424..41941)
                     /gene="purF"
                     /locus_tag="OO244_000568"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5662508.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41978..42466)
                     /gene="cvpA"
                     /locus_tag="OO244_000569"
     CDS             complement(41978..42466)
                     /gene="cvpA"
                     /locus_tag="OO244_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5662509.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42725..43387)
                     /gene="dedD"
                     /locus_tag="OO244_000570"
     CDS             complement(42725..43387)
                     /gene="dedD"
                     /locus_tag="OO244_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5662510.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43377..44645)
                     /gene="folC"
                     /locus_tag="OO244_000571"
     CDS             complement(43377..44645)
                     /gene="folC"
                     /locus_tag="OO244_000571"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5662511.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44715..45629)
                     /gene="accD"
                     /locus_tag="OO244_000572"
     CDS             complement(44715..45629)
                     /gene="accD"
                     /locus_tag="OO244_000572"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5662512.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45785..46444)
                     /locus_tag="OO244_000573"
     CDS             complement(45785..46444)
                     /locus_tag="OO244_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5662513.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46527..47339)
                     /gene="truA"
                     /locus_tag="OO244_000574"
     CDS             complement(46527..47339)
                     /gene="truA"
                     /locus_tag="OO244_000574"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5662514.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47339..48352)
                     /locus_tag="OO244_000575"
     CDS             complement(47339..48352)
                     /locus_tag="OO244_000575"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5662515.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48418..49554)
                     /gene="pdxB"
                     /locus_tag="OO244_000576"
     CDS             complement(48418..49554)
                     /gene="pdxB"
                     /locus_tag="OO244_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5662516.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49653..50648
                     /gene="flk"
                     /locus_tag="OO244_000577"
     CDS             49653..50648
                     /gene="flk"
                     /locus_tag="OO244_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5662517.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50645..51823)
                     /locus_tag="OO244_000578"
     CDS             complement(50645..51823)
                     /locus_tag="OO244_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5662518.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52078..53319)
                     /gene="fabB"
                     /locus_tag="OO244_000579"
     CDS             complement(52078..53319)
                     /gene="fabB"
                     /locus_tag="OO244_000579"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5662519.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPHFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53478..55484
                     /gene="mnmC"
                     /locus_tag="OO244_000580"
     CDS             53478..55484
                     /gene="mnmC"
                     /locus_tag="OO244_000580"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5662520.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55605..55883)
                     /locus_tag="OO244_000581"
     CDS             complement(55605..55883)
                     /locus_tag="OO244_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5662521.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55917..56465)
                     /gene="epmC"
                     /locus_tag="OO244_000582"
     CDS             complement(55917..56465)
                     /gene="epmC"
                     /locus_tag="OO244_000582"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5662522.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56465..57274)
                     /locus_tag="OO244_000583"
     CDS             complement(56465..57274)
                     /locus_tag="OO244_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5662523.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57274..58098)
                     /gene="mepA"
                     /locus_tag="OO244_000584"
     CDS             complement(57274..58098)
                     /gene="mepA"
                     /locus_tag="OO244_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5662524.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58102..59187)
                     /gene="aroC"
                     /locus_tag="OO244_000585"
     CDS             complement(58102..59187)
                     /gene="aroC"
                     /locus_tag="OO244_000585"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5662525.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59222..60154)
                     /gene="prmB"
                     /locus_tag="OO244_000586"
     CDS             complement(59222..60154)
                     /gene="prmB"
                     /locus_tag="OO244_000586"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5662526.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFTIYKD"
     gene            60320..60871
                     /gene="smrB"
                     /locus_tag="OO244_000587"
     CDS             60320..60871
                     /gene="smrB"
                     /locus_tag="OO244_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5662527.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60975..61865)
                     /locus_tag="OO244_000588"
     CDS             complement(60975..61865)
                     /locus_tag="OO244_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5662528.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61852..62376)
                     /locus_tag="OO244_000589"
     CDS             complement(61852..62376)
                     /locus_tag="OO244_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5662529.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62373..62843)
                     /locus_tag="OO244_000590"
     CDS             complement(62373..62843)
                     /locus_tag="OO244_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5662530.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62840..63388)
                     /locus_tag="OO244_000591"
     CDS             complement(62840..63388)
                     /locus_tag="OO244_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5662531.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63393..64115)
                     /locus_tag="OO244_000592"
                     /pseudo
     CDS             complement(63393..64115)
                     /locus_tag="OO244_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64135..64857)
                     /locus_tag="OO244_000593"
     CDS             complement(64135..64857)
                     /locus_tag="OO244_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5662532.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64917..66071)
                     /gene="yfcU"
                     /locus_tag="OO244_000594"
                     /pseudo
     CDS             complement(64917..66071)
                     /gene="yfcU"
                     /locus_tag="OO244_000594"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66020..66775)
                     /locus_tag="OO244_000595"
     CDS             complement(66020..66775)
                     /locus_tag="OO244_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5662533.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66857..67420)
                     /locus_tag="OO244_000596"
     CDS             complement(66857..67420)
                     /locus_tag="OO244_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5662534.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTSTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16920 a        18282 c        16782 g        15675 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacgg aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaataa
      601 cgccttttat tgataattta catatgaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccat cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagtgca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acacagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc tgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt aggcaaagta atcttctatt attaataagc acatcaaaac ctttttgaat
    18841 attaaataat aattaattag catcatcctc attcattaat tccgcttaac aatagtttca
    18901 caccttaccc ccattgcgac acctcggaaa tcatcacgct gttaaactct ccgtaatgcg
    18961 gaaatatcat catcgcgttt cattagtgaa ttcttaaatg aggcattttc acacaattat
    19021 cttacagata aaaaaaccag acttgcaatt aagaattaga acaattcact atataacatt
    19081 gcatgtaaag catataagcc tcattatttt gtcattatta agttattaac agcacaatcg
    19141 agccttcccc tctggcaaaa tcttattctg cagaccttca aaacaccgtc ctgggggagt
    19201 acattgttct aagctgactt ccacggcagg gagtggcgat aacagcaaaa aaggtcaaga
    19261 ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg tctgttatga catccgtggt
    19321 ccggtgctga aagaagcaaa acgcctggaa gaagaaggta acaaggtact gaaactgaac
    19381 atcggcaacc cagccccatt cggttttgac gcgccagatg aaatcctcgt tgacgtgata
    19441 cgcaacctgc ctaccgctca agggtattgc gattccaaag ggctttactc cgcgcgtaaa
    19501 gccatcatgc agcactacca ggctcgtggc atgcgtgatg ttaccgtgga agatatttac
    19561 atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc aggcattgct gaacagcggg
    19621 gacgaaatgt tggttcctgc accagattac ccactctgga ccgcggcggt ttcgctttcc
    19681 agcggtaaag cggtgcatta tctttgcgat gaatcctctg actggttccc ggacctcgat
    19741 gatattcgcg ctaaaattac gcctcgtacg cgtgggattg ttattatcaa cccaaataac
    19801 ccaaccggcg cggtatattc caaagagctt ttaatggaga ttgtggagat tgcacgtcag
    19861 cataatctca ttatcttcgc cgatgaaatt tatgacaaaa ttctctacga cgatgctgag
    19921 catcactcaa ttgcgccgct ggcacctgac ctgctgacca ttacctttaa cggactgtcg
    19981 aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg tgttgaacgg gccgaaaaaa
    20041 cacgccaaag gctacatcga aggtctggaa atgctggctt caatgcgcct gtgtgctaac
    20101 gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt atcagagcat cagtgaattt
    20161 attacccctg gcggtcgtct ttatgagcag cgtaaccgcg cgtgggaact gatcaacgat
    20221 attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt atatgttccc gaaaatcgac
    20281 gccaaacgct ttaacattca tgacgatcag aaaatggtgc tggatttcct gttgcaggaa
    20341 aaagttctgt tggtgcaagg gacggcattc aactggccgt ggccggatca cttccgcatt
    20401 gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga gcaagttcgc gcgtttcctt
    20461 tctggttatc atcagctgta atcttaattt cactgccgga gattgcatcc ggcagtgtta
    20521 tcccgccaca atgacctgat gatgtcatca tacgtaaggt cactatgaaa cagagccatt
    20581 tttttgccca tctctcccgc ctgaaactca ttaaccgctg gccgctaatg cgcaacgtgc
    20641 ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat ggtcgcccat gcgctggcag
    20701 ctatcaaaaa tcggaaattt ggcggtaatg tcaacgccga acgtatcgct ttactggcga
    20761 tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc tactccggtg aaatacttca
    20821 atgcgcaaat cgctcaggag tacaaggcta ttgaaaaaat cgctcagcag aaactggtcg
    20881 atatggttcc ggaagaactg cgggatatct ttgcgccgtt aattgacgag catgcatata
    20941 gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact gtgtgcatat ctgaaatgtc
    21001 tggaagaact cgcggccgga aataatgaat tcttgctggc aaaaacgcga ctggaagcga
    21061 cgcttgaagc gcgtcgcagc caggagatgg actacttcat ggaagtattt gttcccagct
    21121 tccatctttc gctcgatgag attagccagg attcaccgct gtaagcagcc ggagtctgcg
    21181 tcgcatcagt caataagcgc cggatgcgac atcaggctct tgtcaaaacg gaaacagcat
    21241 cgggatcatc accacacaaa ccgccatcac gataatggtg aacggtaccc ccaacttcac
    21301 aaagtcactg aagctgtaat ttcccggacc taaaaccagg gtgttaacag gtgaagacac
    21361 cggtgtcata aaggcggcgg atgctgccat cgccacgacc atcgcaaatg gataaggcga
    21421 cactcccatc gttttggcag cagccagcgc aatcggagcc atcaacaccg ccgtcgcggt
    21481 attagagata aacagcccaa taaccgccga caagacaaac aaacacccca gcatcatatg
    21541 tggcccataa ccgccgccaa tatccatcag ccctttcacc gccagcgcga cacctcccgt
    21601 tttctgtaat gccacagcaa acggcatcat cccaacgatc aaaataatgc tcggccagtg
    21661 aatggattta taggcgcttt cagcatctat acagcggaat ttccccatca gcaggcaggc
    21721 gatgatagcg gcaacaggat taggaatttc atttgtcagc attaacgcca ccattagcac
    21781 cagacagaaa atggcatggg gtgcctggct gtgcgcgggc gatgcttcac tcacctcttc
    21841 cggtaagttc agcgctacga agtcgcggcc ctgtttggcc agcataccga tcagtttcca
    21901 gttacccaca accaggatga tatcgcccag cagcagaggc tcatccgcca gcgaaccttc
    21961 cagcgccacg ccattgcgct tgagccccac cacattcagt ccgtagcggg tacgaaaacc
    22021 aatttcgcgc accgatttac caatcagttc tgactcagga attaatgaaa tctctgccat
    22081 gcccacatca agggcctggt cagaaaaata ctcgccgcgc agtaccatcg gctccagcaa
    22141 ttgctcacta caaaattgcc ggagatcgac atcagccgca gacatatcaa taagcaaaac
    22201 gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg ataacgcgac gaaaacgccg
    22261 ccagcgttca acaccgatga cgttagcgcc ataacgctca cgtaatttga gatcatccag
    22321 ccgttgacct atcattggcg atccggggcg aatagccaga cgtcgcgcac gcccggtcag
    22381 tcgatattca cggataagat cgcgaaaggt tcgacgcgtc cagccttcgc gctgcggggt
    22441 ctgggtatcc cctttcagca tgaaacgcat cactaacata tacaaaatac ccagcaccag
    22501 cacgaccagg ccaataggtg ttacgctaaa gaaactgaag ccgtgatagc cttcacgcag
    22561 caattcactg ttgactacca ggttcggcgg cgtcgccacc agcgtcatca tgccgctaat
    22621 cagcccggca aaacttaacg gcatcatcag acgtgacggc gaagtttgca tacgcatggc
    22681 aacgcttaac accacgggaa taaagatagc gacaacgctg gttgaactca taaacgcgcc
    22741 aagccccgcg acggtcagca tcaacaaaac caacattttg atttcactgt tgcccgccac
    22801 tttgaccagc catgttccca ttacggtggc aacaccggta cggaccaaac catcgccaat
    22861 aataaacaag gcggcaatca ggacaacgtt aggatcagaa aagccggaaa atacttcggg
    22921 gaccgtcagc gttccgctta atgcaaacgc gacaataaca aacaaagcga ccgcatccat
    22981 acgcactctg cccgtcgcaa acaagacgat ggcaacggcc agtaatgaaa gaacccaaat
    23041 caattcaccg ttcacaactt atccttgtta attgaggggg atgacttgat tctgccataa
    23101 aaaagccccg acgagacggg gctaaatcat gatcaggtgt ttcactgaat aataacatcg
    23161 ccatttggct gtttggtcac agtaatttgc tccagactgt ggaggaccaa atcgacctca
    23221 ttcaggcgcg gggtatctgc cggagcgtta accgcaatga catggcaacc cgccgccagg
    23281 ccagaaagca cgccagcggg agcatcttcc accaccacac actcctgcgg cgcaagcccc
    23341 agcagctgcg cacctaacag atacgcatca ggttctggtt ttccgcgctt cactcgctca
    23401 gcggttacaa acacctctgg tgcgggaagc cccgctattt tatggcgcgc tcgcgctacc
    23461 ggcatggagc cagaagtcac aatggcccac ggaatacctg ctttattcaa atgattgagt
    23521 aaggcgattg ccccaggaag cgcggtaata ccttcggttt ccgtggcctc gatttgctcc
    23581 agacgcgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaaa
    23641 gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga
    23701 cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca
    23761 tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcgt tgataatttg
    23821 attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaca cattaagcat
    23881 gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccatgagtcc cagcgtcaaa
    23941 atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc
    24001 agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg
    24061 caaattagac caggaaacat acaacgcatg atacatatcc ataatgtcga tgatagtgcg
    24121 gcaggtttct tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta
    24181 accacgctca ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat
    24241 agtcatcatc ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt
    24301 tgactcctgt atcactctac tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat
    24361 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
    24421 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
    24481 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttaagg attgcagggg
    24541 gtaatggcgt tacagaacgc ttgcccagcc accacaatcc ctgcatgggt aaacttaagg
    24601 cgaacagtgc agtggcaact gccggcccaa gcagaccgcc cagggcaatc tgccagcaga
    24661 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
    24721 gattttcgac aaagaccgga gcaaggcgtt tttccagtgg ccacgtcttt gagtaatgct
    24781 gtccgcggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
    24841 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
    24901 cgttcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
    24961 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
    25021 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
    25081 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
    25141 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
    25201 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
    25261 tttctggttt agccgaatgt ttccacctgc ccgaagcacg tatcaaatgg aaaatggacg
    25321 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
    25381 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
    25441 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
    25501 ttcagggtat caaagatgca gcttcttttg caccgctgca caacccggct cacctgatcg
    25561 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
    25621 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccgtacaacc
    25681 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
    25741 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
    25801 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
    25861 tgggtctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
    25921 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
    25981 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
    26041 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
    26101 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgcat
    26161 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
    26221 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
    26281 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
    26341 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
    26401 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
    26461 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
    26521 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
    26581 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
    26641 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
    26701 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
    26761 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
    26821 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
    26881 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggtg gtgccaaaaa
    26941 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
    27001 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
    27061 tgatccggcg aagctgcaag aatccagccc gctgccggtt ctcggcgctg tgccgtggag
    27121 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
    27181 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
    27241 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
    27301 tgacgtgctg gtggccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
    27361 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
    27421 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
    27481 gagcttcaac ctggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
    27541 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
    27601 ccgtcgtctg tctccgccag cgttccgtta tcagctgact gaactggcgc gcaaagcggg
    27661 caaacgtatc gttctgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
    27721 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
    27781 tgcagcgtct cagggtgtag aactaggtgc aggcattgaa atcgttgatc cagaagtggt
    27841 tcgcgaaagc tatgttggtc gtctggtcga gctgcgtaag aacaaaggca tgaccgaaac
    27901 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
    27961 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
    28021 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
    28081 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
    28141 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
    28201 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
    28261 agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
    28321 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
    28381 ggttgcaggt cgcgctaccg tgttcatctt cccggatctg aacaccggta acaccaccta
    28441 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
    28501 caaaccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
    28561 gctgactgcg attcagtctg cacagcagca gtaatctcgt catcatccac agctttgcgc
    28621 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatttgcat
    28681 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catctcactt
    28741 tgcatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
    28801 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    28861 tattttaacc agtcttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    28921 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    28981 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    29041 cgatgaacgc ccgggcctga tgaacttccc gtttgaaggg ttaacctcag gatcgaaata
    29101 cgggacagcc gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    29161 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    29221 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    29281 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    29341 ctatgacagt attaccaccg tcctggtgac ttatattgcc acgcaaatcg gttttgccag
    29401 ttcgtggatg aacccgtttt gtgtggttgt tgctcagggg attgccggcg ttccggtgct
    29461 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actctgattg gcctgatctt
    29521 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    29581 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    29641 ctggctggta ttgattgtcc tgacagccgt aatggtctgg gtgatttggg gcgtgatcgt
    29701 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    29761 catcatcggc gttgttttcc gccttaacgg catgacggtt aataccatgg cttcatcctt
    29821 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    29881 tttgctgctg gttggtaatg gtgaagcagg taatgccagc gtgttaaata ctatcctcaa
    29941 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    30001 cttccaggct gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cattaactat
    30061 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    30121 gttccagttt ggtgatggct tcagccacat catttaccca acctcagctt cgttaatggc
    30181 aacgctcggt gtgtgccggg tggacttccg taactggctg aaggtgggcg cgaccctgct
    30241 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    30301 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    30361 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    30421 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactc cactgacttc
    30481 atcttcctgt agggcgaagg gaccgtgaga gacgcaactg aacaacgcgc cccagacacg
    30541 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    30601 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaactac
    30661 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    30721 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    30781 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    30841 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    30901 tggcgctctt acggcgcatt ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc
    30961 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    31021 acccgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    31081 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    31141 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    31201 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcatca tttgatccac
    31261 ttcgctgtcg cagttgccac gcacagcgat aaccttatgt gccacttcat taagccgttc
    31321 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    31381 gtcgccaagg atcaccagcc actgggcacc gctttggaca aacaactcca caacacgttc
    31441 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    31501 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    31561 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    31621 ccaggcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    31681 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct aacaaatgtt
    31741 ctgccatcgc gaacagtttc tctgcactgg cttttccctc ggccgttagt ggcgcttttt
    31801 tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg gatgggcatc agatcgctgc
    31861 gcagccaggc ctgaatctgt cgtgcacgcg cacgattttc taaatcaagc ggataaatac
    31921 gttcccaggt cggtggcgca aatcgatctt ccagatactc cgcaatggca gaagattcac
    31981 tcaactcaaa atcatcgatt tgtaataacg gcacacggcg tgtctgaccg taaccttgcc
    32041 acgtcggctg caaatgttca ccgctgtcga ggtcgatggt cttgatatga aacgacaggc
    32101 ctttttcctg caacgccacc caggcggata acacataagg ggagaaaaag tgggcatctg
    32161 accaaagcgt gatagcgggt ttactcatta tgtcctcggc aataaagggg gctggctatc
    32221 aaactataga gcctttttgt cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt
    32281 tctatacttg tggtgttcag atgattaaac aaccggagct gcaatgatcg atctctattt
    32341 cgccccgaca cccaatggtc acaaaattac gctgtttctc gaagaagcag agcttgatta
    32401 tcgcttgatt aaggtagacc tggggaaagg cggtcagttt cgtccggaat ttttgcgcat
    32461 ttcgcccaac aacaaaattc cggcaattgt tgatcattct cctgccgatg gcggcgaacc
    32521 gttaagcctc tttgagtctg ctgccatttt gttgtatctg gctgagaaaa caggactctt
    32581 tttgagtcat gaaacgcgtg agcgcgccgc cacattacag tggttattct ggcaggtagg
    32641 cggactgggg ccgatgcttg ggcaaaatca tcattttaat cacgcagccc cccaaaccat
    32701 tccttacgct attgaacgtt atcaggttga aactcagcgt ctttaccatg tactaaacaa
    32761 gcggctggaa aactcgccct ggctgggagg cgagaactac agcattgcgg atattgcctg
    32821 ctggccgtgg gttaatgcct ggactcgcct gcgaattgac ctcgcaatgt atccggcagt
    32881 caagaactgg catgagcgga tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc
    32941 acaactcggt gatgagcgtt cggatagtta acagaaacag gttctcgtgt attatttcat
    33001 cctaagtaaa acaacggaga acctgcaatg gcacaacctg ccgctattat tcgtataaag
    33061 aaccttcgtt tgcgtacgtt tatcggaatt aaggaagaag aaattaacaa ccgtcaggat
    33121 attgttatca atgtgacgat ccactacccc gccgataaag cgcgcactag cgaagatatc
    33181 aacgatgcgc tgaattatcg caccgtaacg aaaaacatta ttcagcatgt agagaataac
    33241 cgtttctctt tgctggaaaa attaactcag gatgtgctcg atatcgcacg tgaacatcac
    33301 tgggtgacgt atgctgaagt ggagatcgat aaactgcacg cgctgcgcta cgccgattcg
    33361 gtatccatga ccttaagctg gcagcgttaa tcgccatatc gggaggctgc atgaacatag
    33421 tgatcaccgg agggacggga ttaattggtc gccatttgat tccgcgtttg ctggagctgg
    33481 gccatcaaat tacggtagtg acgcgtaacc cgcagaaagc cagttccgtt ctcggccctc
    33541 gggtgacact atggcaaggg cttgccgatc aaagcaacct caacggcgtt gatgcggtaa
    33601 tcaacctggc cggagaaccg attgctgata aacgctggac tcacaagcaa aaagagcgtc
    33661 tctgccaaag ccgctggaat atcacgcaaa aactggtcga tttgattaat gctagcgaca
    33721 cgccgccgtc ggtactcatt tccggctcgg caacgggcta ttatggcgac ttaggtgaag
    33781 tggtggttac cgaagaggaa ccgccgcata acgaatttac tcataaactc tgcgcccgct
    33841 gggaagagat tgcctgccgg gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgaaccg
    33901 gcgtggtact ggcaccggac ggcggtattc tcggtaaaat gctgccaccg tttcgtcttg
    33961 gcctgggcgg gccgattggt tccggtcggc agtatctggc ctggattcat attgatgata
    34021 tggtcaacgg cattctctgg ctgctggata acgagctgcg cgggccattt aatatggttt
    34081 cgccctaccc ggtacgcaat gaacaatttg cccatgcgct cggtcatgcg ctgcatcgcc
    34141 cggctatttt gcgcgtcccc acaaccgcca ttcggctgtt aatgggcgaa tcttcagtac
    34201 tggtattagg tggacaacgc gcgctgccta aacggctgga agaagcgggt tttgcgtttc
    34261 gctggtacga tttagaagag gcgctggcgg atgtcgttcg ctgatgtggt ttacagcaaa
    34321 catccgccag ttaactccct gtgttacagg attagtggct ttgcgcgata agatcgtctg
    34381 gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa acctctatcc agcatctcct
    34441 gagcaatacg cagggcttct tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt
    34501 cagcaatggt catcagtttc tccttttctt gtggtgcgcg ttctgttatc tcaccaataa
    34561 atgcacggaa acgctgggca tcccctgttt gtaatacgta attaaacagg gcttttagct
    34621 gtctgtcatt agtgttccct gtaactagca gcgaaacaat ttggtcgact aatcccaaca
    34681 gatcgcgctg acgaatatgt ttctgaatta actccaacag cgccattttg cggtgttgca
    34741 taatctcgtc atccggcacc acggtaatat ccaccaacgg aaacgctgat gaatatattt
    34801 tgcgggctat agcaggctcg gcaaattcat caagccagca gagtgaataa ggataaggac
    34861 ttctgcaacc atgataaaac agcatcggga tcaccaatgg aagctcttta tagcccgcat
    34921 caagatggtt ttgcattgcc gcaatggaat aacgcatcat gcgaaaagcc attaattctt
    34981 ccggcttact ttggtgctct atcactacat aaatataacc cactccctcc tgcgttttca
    35041 cagaccacaa gaggtcggaa taatattgcc gcaggtcttc atcaataaaa ctgtttggtt
    35101 ctagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg cgcgggaaga tgaatattaa
    35161 taaaattccg cgcggtgtct ggatggcgta aaaaagattt aaataccgca tcatgcggcg
    35221 tggaagttgt cgatattgtc atggcgttcc gtcacccctc aaatttaaga tgacgcgaca
    35281 ataaccacga ttaacaccca atgcagcagg cagtttttat ctttggacag cgaattcaga
    35341 agtattttta gcggactgca acttttacat ttcagcggaa gctgactcgc aaattcccct
    35401 tgaaaatact ccggcagcgt tagccaccgg agaggaaatt atttcagcga tcccttaagg
    35461 aactgttgca gacgagggct ttgcgggttg ccaaataact gctccggcgc gccctcttct
    35521 tctattttcc cctgatggag gaaaatgacg tgagtagaaa catggcgagc aaagcccatt
    35581 tcgtgagtca ctaccaccat ggttttcccc tcttctgcca gttgctgcat aatacgtaac
    35641 acttcgccta ccagttcagg atcgagcgcc gaggtaggtt catcaaacag taaaacttct
    35701 ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc
    35761 accggatatt tcccctgcgc acgttcgtct atcccgactt tcgccagata cttcaccgcc
    35821 cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa tcggcgcttc catgacgttt
    35881 tccaacaccg tcatatggct ccagagattg aagtgctgga ataccatcgt caggcgtgtg
    35941 cgcagtaagc gcagttgatt tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc
    36001 agattgatcg tctggccatt gaccacgatc gatccttcac tcggtttttc gaggaagtta
    36061 atgcagcgca gaaaggtact tttccccgat cccgacgatc cgatgatgct tattacatct
    36121 ccggcattcg cttgcagtga gacccctttc agcacttcgt gttcgccata gcgtttgtgc
    36181 aaatcgataa cgtttaattt attctcggac atcgtattct cagtgcgttg aagaaggttt
    36241 cacatgctgc aaccagcgtt tttccgctct gcgaaagagg ctgatcagga cataagagat
    36301 gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt
    36361 aatatcgcgg gctattttca gcagatccgg cacagtggcc gtaaacgcca gcgcagtaga
    36421 gtgcagcatc aggatcactt cgttgctgta tgccggtaac gcaatacgca gcgcagaagg
    36481 caaaataatg cagcgataca ttttaaaggt cgagaagcca taggctctgg cggcttcaat
    36541 ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt
    36601 gttaagcgtc agcgccagca cggtacagtt caggccactg cggaagaaag cgttaaggaa
    36661 ttcggttccc ttaacaatct caagcgtgta catgccggaa tagaacacca gcaactgaac
    36721 atacagcggc gtaccgcgaa aaatataggt aaataaccag attggaaact ggatgtattt
    36781 attactggag acacgaccaa tcgccagaaa cagcgccagg actccgccta tcactaccga
    36841 caaaataagc agccacagag tgatcgccac accagtaaag cgataaccgt cggtccacag
    36901 cagcggtttc cagtattcat gtaagatttc gatcacaggt cagccctctt cacacccacg
    36961 gagtagcggc gctcaaggaa cagcagcaca ccattggaaa cggtggtgaa aaccaggtaa
    37021 atcacgccac agacgatggc gaaatagaac ggttcccagg tacttttgcc tgccagctgc
    37081 gtggctttga ccacatcttc caggccgagt aacgaaacca gtgcggtaga tttgaggatc
    37141 acctgccagt tgttgccaat gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc
    37201 cgccgaaaca cttgcccgcg agtaaaaccg aacgccgtcg ccgcctctat atgccctttc
    37261 ggcactgcca taaacgcgcc acggaacgtt tcggtaaaat aagcaccgta gataaaaccg
    37321 agagtgataa taccagcgac catcggatcg atatcaatct gcccgacgcc catcgcctcc
    37381 gtcaccatgt ttagcgcaat ctgcaaacca tagaaaatca gcagcatcaa caccaagtcc
    37441 ggcacgcctc gaatcagcgt ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc
    37501 gagagcttac cgccagcgcc aattaaaccg atgattacag cgagcactac agagctgata
    37561 gccagctcca gcgtgacgag cgcaccctgt aaaataacac ctgaaaaccc atacaacatg
    37621 ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt ctccctccgg ttacccgaag
    37681 ggagcgggct ttctttcacc gatggcgatt aaccaccata aacatcaaaa tcgaagtact
    37741 ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg
    37801 cctcgcgcag ttcgttatct tctttacgca ggcccatgcc ggtccctacg ccaaacagtt
    37861 tttcatcttt aacagacgga ccaccgaatt tgtaatcttt accgacgggt tgtttgagga
    37921 aaccttcgct ggcagcgacc tcatcctgga acgcggcatc aatacgtccg gcagtcaggt
    37981 cagaataaat gttgtcctgc ccctgatacg agacgatttc aatgcctttt ggtgcccagt
    38041 gttcattacc gaacgtctcc tgggtggtgc cctgcaatac gcctacccgt ttgcctttca
    38101 gcgactcgac tgtcggctga atgtcagaat ttttcgccac caccagacga gaatcggcag
    38161 cgtacagttt gtcggtgaag gcaatttctt gctgacgttt ttccgtaatg gaaagcgatg
    38221 acatgatggc gtcaatcttc ttcgctttta aggacgggat taacgcatcc agcgggtttt
    38281 cgacaaacgt acattgcgta ttgatgcgct tgcacaactc cttcgccagc tcgatatcga
    38341 aaccaaccag ttcgccttgt gaattttttg attcaaatgg cgcataggtc gggtcggtac
    38401 cgatgcggat gttttgcgga attgcagcaa gtgccgcagt tgcgctggag aaggccagaa
    38461 ccagagagag cgatagcacc agttttttca taatgtcctc aactgacagt ctttttatag
    38521 ggaatattta caggtttgtt acacttatcg tgccataaaa ttgaccatca aggcaaaata
    38581 tcccgccccg ttagacgtat tatttttcat taaaaatgct taagtatgca tttcaatgca
    38641 ccacgatagt gcaacaaacc tggcgtgcac catgatgatc attccatcag gtacagcttc
    38701 ccagcgacgt atcagtcacc gtagacatta aagtcgaaat actttttcgc catcttgtcg
    38761 taggtgccgt cctgacgcag ctcgccaagc gccttattga aggcagccgt cagttcagca
    38821 taatctttac gtagccctac accggtgcca tcaccgaagt attttttgtc ttttactgat
    38881 gagccagcaa aagcgaaatc tttaccagca ggttgcttga ggaatccttc gctggcagca
    38941 acttcatctt gtaacgcagc atccagacgt cctgcagcca gatcggaata gaccaaatcc
    39001 tggttggcat aggccaccac atccacgcct ttactacgcc aggtctcgtt agcgtaagct
    39061 tcctgggttg atccctgcag cacaccaaca tgtttacctt tcagtgaatc cagcgttggc
    39121 tgaatcggtg aacctttggc agcaatcaaa cgagaatctg cggcgtacag cttgtcggag
    39181 aaggcaatct cctgctgacg tttatcggta atggaaagcg acgaaataat agcgtcgatt
    39241 tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc
    39301 acctgcatcc gtttgcacat ctcgttaccg agatcgatat caaagccaac aaaatcacct
    39361 ttagcatctt tcgacgagaa cagcgcatag gtggtatcgg ttccgatacg taccgtctcc
    39421 ggtagcgccg catagctgga agccgctgcg gagagaccga ctaataaaga cagagcgaga
    39481 atcgacttct tcatacataa ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt
    39541 tgtgtgtttg caggctcttt catgcaggtc ttatgccatc ttgacgacaa cagtaacatt
    39601 caacgttaaa tatgttaata agacattgca ttattgtcct gaagttgaag atagcaggta
    39661 tagcggttga atcgcagcgt ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc
    39721 gcggttgcaa aattgccctg aaacagggca acagcggagt tatgcgccct gccagcgggc
    39781 aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga acacgattaa ccgtctgatt
    39841 tatcacatca tcaagggatt gcgggcgatg ataaaacgcc ggaacgggag gcataatcac
    39901 cgcaccgatt tctgccgcct gagtcattaa acgcagatgg cctaagtgca atggtgtttc
    39961 acgcacgcag agcaccaacg ggcgacgctc tttcagcacc acatctgccg cacgggtcag
    40021 taagccatca gtatagctat ggacaatgcc ggacagggtt ttgattgaac agggtaaaat
    40081 caccatcccc agcgtctgga aagaaccgga agagatgctg gcggaaatat cgcgcgcatc
    40141 gagcgtgaca tcagctaatg cctgcacttc gcgcagggaa aaatccgttt cgagggataa
    40201 ggtctggcgc gccgcctggc tcatcaccag atgcgtttcg atatctgtga catcgcgcag
    40261 aacctgtaat aagcgcacgc cataaatcgc gccgctggca ccgctgatgc ctacaatgag
    40321 tcgtttcata aatagttgcc ctgtcagact tgcaggcaga ctttgcagta tttcggcggg
    40381 agttgcaagt cagggtgcca gaccggcacc ctcagcgaag gcatcatcct tcgttatgca
    40441 tttcgagatt ttccacttcg ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg
    40501 tatcgaggaa atcgaggtag ccctgatcaa catctttggt gacgtagacg ccgttgaaca
    40561 ccgagcattc aaactgctgg atatccggat tttcagcgcg aacggcgtcg atcagatcgt
    40621 tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc
    40681 gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat accataaacg ttcgggaagc
    40741 gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct
    40801 cgataatctg ctcagaagtg gtgccacgga cgatggagtc gtcgaccagc aggacgtttt
    40861 tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg cacggactta cgacgcagct
    40921 gctggcccgg catgataaag gtgcggccaa catagcggtt tttaacgaag ccctggcggt
    40981 acggtttgcc cagaatacga gcaatttcca gcgcgatatc acacgaggtt tctgggatcg
    41041 ggatcaccac gtcgatatcc agatcttccc attcgcgggc aattttctcg cccagtttcg
    41101 tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc gataaacgag tccgggcggg
    41161 caaagtatac atactcaaac aggcacggat tgctgaccgg attgtcagca cattgacggg
    41221 taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga
    41281 aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc gaccatatat tctgtacggt
    41341 tctcgtcaat atcacgtttt cccagtacca gcggacgaat cccgtttgga tcgcggaaag
    41401 caaccatacc gtggccgata atcatcgcca cacaggcata cgcgccgcgg attaagcggt
    41461 ttgtggcagc aatggcagcg aaaatattgt cggcttccag cgggtagtgg cggaagttgt
    41521 ccagctcgct ggcgaagata ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc
    41581 ggcgtttttc ttcaaacagt tttttacgca actcgtgagc gttggtcaga ttgccgttgt
    41641 gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg
    41701 agctgccagc cgtagggtaa cgcacatgac caatgcccat attgccctgc aaacgctgca
    41761 tatggcgagc ttcaaataca tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt
    41821 tattggcatc tatggtgatg atgccggcgg catcctgacc gcgatgctga agcaccgtta
    41881 aggcatcata aatcgactgg ttaaccggca taacaccggc gataccgaca ataccgcaca
    41941 tacgtctttt tcctcgttaa gccacatctc agagcactta cgctctgggc aagaaacttg
    42001 acgagctttg cagataatca aaaaaccatc tgatgataaa actgaattgc gggatcagct
    42061 gtgatttgct ccagtcttca cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa
    42121 tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg
    42181 tgcctgacaa ccccgttttc tccaccaact ggcctatcac gaagttcacg atagcaccaa
    42241 cgatcagggt agcgataaac agtaccgcga tggcaatccc atttcgaacc agttcgtctt
    42301 caaagcccgt aaaccagact gacaggtaag tgtagtaatg actggcaaca aagaaagcac
    42361 aaccccatgt caccagcgat aacgcttcac gaacaaagcc gtggatcagg ctaaccagag
    42421 aggaaaaagc aatcaccgcg attatggcgt aatcaatcca gaccatatgt gtcccacgat
    42481 tttacgccct gtcatcctgt tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag
    42541 ggatttcctt cccgcgcatc aataaaaatg gcgctgaaaa aatattcaac gccatcgact
    42601 ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc ggcgtaaacg ccttatccga
    42661 cctacggttc taacactgcc caagcctgat aagacgcgcc agcgtcgcat caggcaagac
    42721 tgtattaatt cggcgtatag cccattacca cgccacttaa gccagaaagt tgcttcaact
    42781 cacccagcga acctttcagc ttatctttcg aggcatccgg cccaaccaga atacgggtaa
    42841 ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct
    42901 taccgacaat ctcattcact ttatcggcat ttttcagcgc acccagttgc acaacatagg
    42961 ctttacccgt tggcgctgct ttttcttcca cgaccggctt cggttctggc ttcggcgcag
    43021 gtggtacttc aacctttggt ttaggcggct ccaccggttt cggcttcggt ggggcgaccg
    43081 gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg
    43141 gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca
    43201 acgcctgggt ggcggctggc atcatatcag gctcatcacg atcgcccgct ttcggcacca
    43261 gcgggatagc cgcgaactca tcctgataat gttttttctg cccgtccagc agccctggaa
    43321 gtacaatcac ccccagcgcc accagcacga tcgtgcccac taaccgattc tgaaacttac
    43381 ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa
    43441 ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc
    43501 gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca
    43561 ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc
    43621 agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt
    43681 ttcggtagcg ctttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca
    43741 tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg
    43801 gcaatcccgt cgcgaatggc attttcactg acttccagcc cgctggcacg cagtgccgcc
    43861 agcgccgttg cggcattcgg ttgcgggaca agcggcaacg gcagattttc cagcgtgccg
    43921 tgagcatcgc taaacgccca gtcatgatcg gtgacggaat agttccactc aacgccccga
    43981 cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catttccggc
    44041 tcaccgacaa ttgccggttt ttcgctgcgg aagatgcctg ctttctcgag accaatactt
    44101 tcgcgatctg gacccagcca gtcggtatga tccagcgcaa tactggttac taccgcgaca
    44161 tcggcatcga caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcacc
    44221 acgtcaagtt gtgcctgctt gaacagccac aacgccgaca gcgtaccgta ctcgaaatag
    44281 gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaggcggt gtgggccgat
    44341 tccggcaatt cctggccctg cacacgtacg cgctcggtat aacgcaccag atgaggcgaa
    44401 ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagcgt acggcaggtg
    44461 gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt caggacgcca
    44521 agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgcagg
    44581 ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata
    44641 atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct
    44701 gcccttatca gttatcaggc ctcaggttcc tgatccggta ccgggggtac cactacgcct
    44761 tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc
    44821 agtttcaggc gcatttccgg acgacggacg atcatgtcga tcgcgccttt ctcgatcagg
    44881 aattcactgc gctggaatct aggcggcagt ttttcgcgaa cggtctgttc gataacacgc
    44941 ggaccggcaa agccgattaa cgctttaggt tcagcgatgt tgagatcgcc cagcatggcg
    45001 aaacttgcag aaacaccgcc catcgtcggg tcggtcagca cggagatgta cggcaagccg
    45061 cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt
    45121 gcttcctgca tacgtgcgcc accagaggcg gagaagcaga tcagcgggca gttatcttcc
    45181 tgcgcctgct caacggcacg cacgaaacgt gcacccacaa cagaccccat tgaaccgccc
    45241 ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc
    45301 accaccagcg catctttttc gccggtttct ttctgcgcag atgccagacg gtctttatac
    45361 ttcttggagt cacgaaactt cagctcatct ttcggctcaa gctcgctacc cagctccaca
    45421 aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg
    45481 tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg
    45541 cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg
    45601 ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat
    45661 gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt
    45721 gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta
    45781 ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg gattatttcg atgacgcccg
    45841 gcaaaataga aaccacaata atcccgacga tcagcagctt aaggttatcc tgaaccatcg
    45901 ggattgtacc gaagaaatag cctgcgtagg taaaaagcag tacccacaac agtgcgccga
    45961 tcacgttata agcagcgaaa tgacggtacg acatgtggcc cattcccgca acaaacgggg
    46021 cgaacgttct gacgatcggc acaaaacggg cgagaataat cgttttgccg ccatgtttct
    46081 cataaaactg atgggttttg tcgagataac tgcgacggaa aattttcgaa ttgggattac
    46141 tgaataactt ttcaccgaac aaccgcccga tagtgtaatt gaccgcgtca cccacaatcg
    46201 cggcgatcag catcagcact accatcatat ggacgttgag atcgttggtt tccagtgatg
    46261 ccaacgctcc ggcaacaaac agcaatgaat cacccggtaa aaacggcatt accaccagac
    46321 cggtttcaca gaacaaaatt aagaacaaaa tggcataaac ccagacgccg tactccgcga
    46381 ctagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt
    46441 ccatatttgc ctatgccttg tactcgtcat ctttcaggct gtaactacgt tggctacagc
    46501 ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt
    46561 tttggaagat cataccggtc agggtaatcc accgcgacca gatacagccc ttccgctttt
    46621 gccgttgctg ccgccagcgt tctgtccttt gccgccagca actctgctat ccagctctcc
    46681 ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt
    46741 acaaaggcat tcgctttgat atcaaccacc acataaggac cgtgacgcgt gacattaata
    46801 tgcataacgt tgcgccacgg ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa
    46861 tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg
    46921 taaaaatggg ttaccccttt actcagtacc gccgggcgca gccgatgatt gtagatgatg
    46981 tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta
    47041 acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg
    47101 tctttgcgct gcgcggttgt ttcgaaatgc accacttgcc cggtaccgtg tacccctgcg
    47161 tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt
    47221 tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta atacttactg
    47281 ccgtcgtact caatgcccag cgcaatttta taaactggca gttgttgctg gtcggacatt
    47341 agtacagata ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa
    47401 agcgaacgtt atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac
    47461 gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc ccctacctga gtcgggaatt
    47521 cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg
    47581 ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag aataccggtg
    47641 cctggacgac gcttgccgaa atcatcagcc cttcgtcctg caggattttg cgtacttcgt
    47701 caacgatacg acgttcttca cgcacgctac cttcgctatc cggcaacaac ggcagcatat
    47761 tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt
    47821 tcgcactctg ccccgctaac gcatcgaccg cttttttgcc ctgggcggag gctgaaatca
    47881 ggctggtaac actgatacgc gacaaaccgc cctgatcgat taacggtttc agtgccgcca
    47941 gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca
    48001 gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga gcaaacagtc
    48061 cgctgctgtc gatcaccagg caacctgagt tggtcgcttc ttcaacccag gcagcggtag
    48121 cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat tcagcggcat
    48181 cctgcacggt gattgtttta ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg
    48241 ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt
    48301 cgcccatagc gccagttgcg cccaggacgg caatgttcca gccttcagac atggtggttt
    48361 actccagaaa tagcaaagct ccctgccaat gttacagcag agagcatgaa gaagagatta
    48421 acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc
    48481 gtcatcacaa attacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag
    48541 tttatcgaac tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac
    48601 atcatacacc aaatgcacca gccttttcag cgtcggttga tcgagcggac catgcagcgt
    48661 aatgcggcca aactcaggcg cgggcagcaa cgtatccagc gcaacgtgtt gttcatgccc
    48721 aataaacttg ctataggctt caaacacttg cgtcgtgcca cgcgctttac cctccagggt
    48781 atagcctgcg atatgcggcg tgccgatatc cacttttgtc agcaattcca cattaagttc
    48841 cggctcgcct tcccagacat ccagcaccac gcttaacttc tggccttcat tcaggcaggt
    48901 cagcagcgcg gtattatcga cgactgcgcc acggcaggcg ttaatcagaa tcgctccggg
    48961 cttcaggcta cggatcagtt tttcatccgc cagatgtagc gttttgtacg gaccatcttt
    49021 aaagagtggc gtatggaaag tcagaatatc cgcgcgctgg actaactcat ccagcgagcg
    49081 gaaatcaccc tcatccccac ggtcggcgcg aggcggatcg caaagtaagg ttttaatccc
    49141 taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta ccaacgccca cgatccccac
    49201 cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc atcagcaggg aggagaaaac
    49261 atattccacc accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg
    49321 cttcaaccat gcttcatcga catggtcggt ccccgcagtg gcagttccaa caaatttaat
    49381 gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca tcagcgcatc
    49441 cgcgtctgcc agttgagcga cggggattgg acgcccggga accgcgatca cctcacccaa
    49501 acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt
    49561 gttacctgta tgagacgaga gttaaccgga caagtatgcc ataatctcgc ggccaggcat
    49621 acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc
    49681 ctgggcagcc accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat
    49741 ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac
    49801 aacaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac
    49861 cgctgctttc gcgtcacttc cctgccgctg agcataatct ggcgcaacgt ctgctggccg
    49921 cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg
    49981 ggaatcatcg tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga
    50041 atcagctctc accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg
    50101 ttattccgca accacagcag cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca
    50161 atgcgctaaa acagctggtg accaaacttg cggcggcaac gggggaaccc agcaaacaga
    50221 tctggcaatc gatgctggaa ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac
    50281 tgtttaacca tctggtgacc tggctacagg cgcgtcagac gctaagccag caaaatacgc
    50341 cgacgctgga atcactacag atggcgctaa aacaaccttt agatgccagt gaactggcgg
    50401 cgttatcggc atatatccag caaaaatatg gcctttctgc gcaatcatcg ctttcttctg
    50461 cccaggccga ggatattctt aatcagcttt atcaacggcg ggttaaaggg attgatccgc
    50521 gtgatatgca accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg
    50581 caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgatgctg gtctggctgg
    50641 ttcgttaacc ccgacgaaat gacagtatcg tgacaataat acccagcacc gcagagatcg
    50701 ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gccagcggcc
    50761 cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa
    50821 cttgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata agcgaacatc
    50881 cggctccggt taacgccgcg cccgctaatg cgacccatgc acctggggct tgccagagca
    50941 gcaacaagcc caccgtttct acaagcagag agacaatcgc cactttcacg ccgccaaaac
    51001 ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa
    51061 gagtaaagcc cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga
    51121 taaccgcaaa accaacgcct tgtagtgcca gccctaaccc tggtttccag ataagcccga
    51181 caacgctcca cagcgatgga cgttctcccg ccagggccgg tactttgcgc actgtgccgt
    51241 tacaggccca cgccagtaag ggtaatacca ttgtggtgat cgccagtgcg gcaaaaccgt
    51301 aatggctatg aatcaacagg ccaagcggcg caccaacagc gagggcaccg taaatcgcca
    51361 ttccgttcca tgacatcact ttgccagagt gttttggccc tacgatgcct aacccccagg
    51421 tcagagcgcc tgtcagtaac tggctttcac caaacccaag aatcaaacgc ccgatgacca
    51481 acagggcaaa tttgaacggt gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc
    51541 cagccagacc acaagctaac atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg
    51601 ccagtcgccc ggcgtaacca cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa
    51661 tgccgaccat ggtattgcca tagcccagat catgatgaac aaacagcggg ataaccggca
    51721 acggcaaccc tacggtcatg taggtgagaa aaaccgcaaa agagatgcgg aagagcgaaa
    51781 aactggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc
    51841 agcgcaaggc gaggagtatc cccgtctcat ctctctggtt tcagggttac ggtgcgttgg
    51901 caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca aagcgggaag tttgcctaga
    51961 actggtggcg attgtcaatg atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt
    52021 gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta
    52081 ctccggccta ctgcgaatta atcttttcag cttgcgcatt accagcgtgg cgttggtgcc
    52141 gccgaagccg aagctgttag acataacagt ggtcagttcg cgatcggtcg tttcggtcac
    52201 gatgttcaga cccgcagcct gctcgtccag ctcttcaatg ttgatgctcg gggcgataaa
    52261 gccgtgttcc agcatcagca gagagtagat agcttcctgc acacccgcag cgcccagtga
    52321 gtgaccggtc atggctttgg ttgcagaaat cgccgggctc ttatcgccga acacttcacg
    52381 gatagctgcc agctctttca cgtcgccaac cggagtcgaa gtaccgtgag agttcaggta
    52441 atcgattggg gtatcaacgc catgcatcgc catctgcata caacgtactg cgccttcgcc
    52501 agacggagca accatgtctg caccatcaga ggttgcgccg tagccaacga tttcagcata
    52561 gatgtaagca ccacgcgcca gcgcgtgttc cagctcttca accactacca taccgccgcc
    52621 gccagcgata acgaaaccgt cacggtgagc gtcgtaagta cgggaggctt tttccggggt
    52681 gtcgttgtat ttagtagata gcgcacccat cgcgtcgaat tcgcaggcca tttcccagca
    52741 cagctcttcg ccgccgccag caaacacgat gtcctgtttg cccagttgga tctgctctac
    52801 tgcgttaccg atacagtgtg cggaagtcgc acacgcggag ctgatggagt agttaacgcc
    52861 atgaatttta aacggcgtgg cgagacaggc agaaacgccg gatgccatcg ctttagtgac
    52921 cacatacggg ccaaccgctt tcaggccgcg cgggccgcgc attgcgtcag cgccgaacac
    52981 ctggaaatgc ggggaaccgc cgccggaacc tgcaatcagg ccaacgcgcg ggttattctg
    53041 gtaagcttcc ggagagaggc ccgcatcagc gattgcctgc tccatagaaa ggaatgcata
    53101 aatggatgcg tcgctcataa agcgcacaac tttgcggtca atgaggccag tggtatccag
    53161 ttttatgttg ccccagacgt ggctacgcat gccggaatcc ttcagctcct gagagaaagt
    53221 gatccctgaa cgtccttcac gcagagatgc caggacttcc tgctggttat taccgatgct
    53281 ggaaacaatg cccaggccag taatcactgc acgtttcatt caatacctct gtaagtcgca
    53341 catagagtaa gtttcgaatg cacaatagcg tacacttgta cgccgaacaa gtccgatcag
    53401 ccatttaata gagatatttg cgcagcctta cacacatcgc taagatcgag ccaccgcctg
    53461 taagacgagt aacttacgtg aaacactact ccatacaacc tgccaacctc gaatttaatg
    53521 ctgagggtac acctgtttcc cgagattttg acgatgtcta tttttccaac gataacgggc
    53581 tggaagagac gcgttatgtt tttctgggag gtaaccaatt agaggtacgc tttcctgagc
    53641 atccacatcc tctgtttgtg atagcagaga gcggcttcgg caccggatta aacttcctga
    53701 cgctatggaa ggcatttgat cagtttcgcg aagcgcatcc gcaagcgcaa ttacaacgct
    53761 tacatttcat tagttttgag aaatttcccc tcacccgtgc ggatttagcc ttagcgcatc
    53821 aacactggcc ggaactggct ccgtgggcag aacaacttca ggcgcagtgg ccaatgccct
    53881 tgcccggttg ccatcgttta ttgctcgatg aaggccgcgt gacgctggat ttatggtttg
    53941 gcgatattaa cgaactgacc agccaactgg acgattcgct aaatcaaaaa gtagatgcct
    54001 ggtttctgga cggctttgcg ccagctaaaa acccggatat gtggacgcaa aatctgttta
    54061 acgccatggc aaggttggcg cgtccaggcg gcacgctggc gacatttacg tctgccggtt
    54121 ttgtccgccg cggtttgcag gaagccggat tcacgatgca aaaacgtaag ggctttgggc
    54181 gcaaacggga aatgctttgt ggggtgatgg aacagacatt accactcccc tgctccacgc
    54241 cgtggtttaa tcgtacgggc agcagcaaac gggaagtagc gattatcggc ggtggtattg
    54301 ccagcgcgtt gttgtcgctg gcgttattac ggcgcggctg gcaggtagcg ctttattgcg
    54361 cggatgaagc ccccgcgctg ggtgcttccg gcaatcgcca gggggcgctg tatccgttat
    54421 taagcaaaca cgatgaggcg ctaaaccgct ttttctctaa tgcgtttact tttgctcgtc
    54481 ggttttacga ccaattaccc gttaaatttg atcatgactg gtgcggcgtc acgcagttag
    54541 gctgggatga gaaaagccag cataaaatcg cacagatgtt gtcaatggat ttacccgcag
    54601 aactggctgt agccgttgag gcaaatgcgg ttgaacaaat tacgggcgtt gcgacaaatt
    54661 gcagcggcat tacttatccg caaggtggtt ggctgtgccc agcagaactg acccgtaatg
    54721 tgctggaact ggcgcaacag cagggtttgc agatttatta tcaatatcag ttacagaatt
    54781 tatcccgtaa ggatgactgt tggttgttga attttgcagg agatcagcaa gcaacacaca
    54841 gcgtagtggt actggcgaac gggcatcaaa tcagccgttt cagccaaacg tcgactctcc
    54901 cggtgtattc ggttgccggg caggtcagcc atattccgac aacgccggaa ctggcagagc
    54961 tgaagcaggt gctgtgctat gacggttatc tcacgccaca aaatccggcg aatcaacatc
    55021 attgtattgg tgccagttat catcgcggca gcgaagatac ggcgtacagt gaggacgatc
    55081 agcagcagaa tcgccagcgg ttgattgatt gtttcccgca ggcacagtgg gcaaaagagg
    55141 ttgatgtcag tgataaagag gcgcgctgcg gtgtgcgttg tgccacccgc gatcatctgc
    55201 caatggtagg caatgttccc gattatgagg caacactcgt ggaatatgcg tcgttggcgg
    55261 agcaaaaaga tgaggcggta agcgcgccgg tttttgacga tctctttatg tttgcggctt
    55321 taggttctcg cggtttgtgt tctgccccgc tgtgtgccga gattctggcg gcgcagatga
    55381 gcgacgaacc gattccgatg gatgccagta cgctggcggc gttaaacccg aatcggttat
    55441 gggtgcggaa attgttgaag ggtaaagcgg ttaaggcggg gtaatctgct ctcgcagtgt
    55501 ttgccggatg cggcgtgaac gccttatcca acctacgtgt gacgtgtagg cctgataaga
    55561 cgcgataagc gtcgcatcag gcatggtgct ccagacgccg caacttacag ctgtgacgcc
    55621 tgctgaaaca ggttttccca catatcggtc accagcgcct ggtcacgcgg cgacaattca
    55681 ccggcaccaa tggctttttc cagactctgg ctaacggtcg tatgcaccgc ctgagcggag
    55741 tggtcatcac cactttccag ttctgcgatg gctaatgtca ggtggccacg caaataccca
    55801 ctggcaaaca actcatcatc actggcatgg tcaaccatgc cgtcgattaa tgccagaatg
    55861 cgtgattcaa actccgcgat catcttcttt cctcattgta aaacgtggcg ctggcttcag
    55921 ttgagcgctt ccggccacgg gaactgttcc gccgtaagtt ccggcgtgtg ataataattt
    55981 tgtagtgctt taatgaagcg tgccgggcgt tccggaatgc catttgtcag ataatccatc
    56041 acctgcgcat gtacccgccg ttgaaacacc acacgatccg gttcgaaatc cccttccaga
    56101 ttgtcgcaac tgacgttaaa cggatatcca gccgccacgc agaataacca gtcgaacgcc
    56161 tgcggcttca cttcaacatc ttcaaactgg ctttgcgtct gggcatcgcg tccgtccggg
    56221 caataccagt agccgaaatc aaccagctca cggcgcgctt ttccggcaat acaccagtgc
    56281 gaaatctcat gaattgcgct ggcataaaag ccatgagcaa agacgattcg gttatacggt
    56341 acttccgcat cagcaggaag atagatcggt tcgtcgtcgc ctttaatcag acgggtatta
    56401 aattcatcgg caaagcagct attaaaaatt tcaatcaact gctcgtagtg gtgtgtactg
    56461 ttcattagtt catccccaac cagtggagga tctcctgtcc gtggctatcg taaagtaatt
    56521 tggcactcat caccgccgag acgataacaa tcatcgggcg gatcagcttt tgtcctttgc
    56581 tcaacaccaa ccgtgaaccc atgcgcgcgc cgaggaactg ccccaccagc atgacaaagc
    56641 ccgtcgccca aatcactttg ccgccgagaa taaacagcag cagaccgcca atgtttgacg
    56701 ttgcgttgag taatttggcg tgagccgtgg ctttggcgag gttgaatccg cacagcataa
    56761 cgaaggccag cgcgtaaaac gatccggcag ccgggccaaa gaatccgtca taaaaaccga
    56821 cgcagccacc ggcaattaac gcgaagggta aaccgtacat ccggcgctgg cgatcttctt
    56881 cacccagctt tggcatcagc aagaaataga ggccgataca aatcaccaga atgggcaaaa
    56941 tctgccgtaa gacatcagcc tgaacgtact gtaccaacag tgcgccgctc attgagccga
    57001 caaaggtcat ggcaatattg agtttctgat cgcttaaact aaccactttg cggcgaataa
    57061 agtagatagt ggcggaaata gagccgccgc aggcttgcag tttattggtt gccagcgcgt
    57121 tagcgggaga cattcccgca gccatcaatg ccggaatggt gagtaaccca ccgccaccgg
    57181 caatcgagtc gataaatccc gccagcatgg cgacaaaaaa gagaactccc agcaacagcg
    57241 gagaaaccat aaacaggcta ttaaacgttt ccattagatc acgtgctcat ccagtagcgc
    57301 ctggcaggaa ggcggcaacg gaggcggtgt cttcttctca ggctttgttg ttcccggttt
    57361 tggaggttca aaccagcttt gcagttctgc cccgcaacca tcgcctgatg gcggtaaagg
    57421 ttgatcttca cactccagac tatcggcagg gcaacgtaat cgtacatgca tatgcgcgcg
    57481 atgctggaac cagggtcgca ctttgcgcaa ccagtcgcga tcggtgcccg catcaaggca
    57541 aagttgttgt ttaatcgccg gattaacaaa aatgcgcgtg acgtctttgt cctgggcggc
    57601 gagtttgatc aagctgaaaa tttctggctt ccacagcgtg gagacaacgt gtttaccgtc
    57661 gcgggaaact aagtccagtg cttgcgggcg caagagctgc gcggaggtcc agcgagtttt
    57721 cggcagttgc agaaagatat cgacatccag tccggtctgg tggctggcat gaccgccgtt
    57781 gaaacgccca ccagcgggca tccccatatc gccaatcagc accgtaccca tgcccagatt
    57841 gctcacctgg ctactcagac gctggataaa catcaccaga tccgggtgac cgaaatagcg
    57901 acgctgatcg gtacgcatga cctgataatg ttcggactgt atcggcaacg tgtcagcgcc
    57961 gacaatacag ccattagaaa aactgcctat cgattgtgcg ctacccggca caggttgggt
    58021 tattttttgc cacggcgtcg ctgccaggct agcactactg gcaagcagag ccagcagcgc
    58081 aatcgcggtt ttgttcattt tttaccagcg tggaatatca gtcttcacat cggcattttg
    58141 cgcccgctgc cgtaacaggt gatccattaa aacgatcgcc agcatcgctt cagcgatcgg
    58201 cactgcacgg atcccgacac agggatcgtg acggcctttg gtgatcatct caacttcttc
    58261 gccaaagcgg ttaatagtgc gccccggcac ggtaatgctg gaggtcggtt tcagcgccat
    58321 atgagcaatg atttgctgcc cgctgctgat accgccgaaa atgccgcccg catggttgct
    58381 ctggaaaccg tctttggtga tttcgtcgcg gttctggctg ccgcgcagcg ccactacgtc
    58441 aaaaccgtcg ccaatttcca cgcctttcac cgcgttgatg ctcatcagcg catgggcgat
    58501 gtcggcatcc aggcggtcaa agaccggctc gccaagtccg gcggggacac cactggcaac
    58561 aacggtgact ttcgcgccga tggagtcgcc ctcttttttc agcgcgcgca tcagttcatc
    58621 taacgcgtcg attttgtcgg ggtccgggca gaagaacgga ttttgttcaa ccagcgacca
    58681 gtctttgatt tccagcggaa tgtcgcccat ctgggtcagg cagccgcgga tttcaatacc
    58741 aaatttctcg gcgagatatt ttttggcaat cgcccctgcc gccacgcgca tggcggtttc
    58801 gcgggcggaa gaacgtccac cgccgcgata atcgcgcaga ccgtattttt gttcgtaggt
    58861 gtaatcagca tggcctggac ggaagacgtc tttaattgcg ctgtaatcct gagaacgctg
    58921 gtcggtattt tcgatcaaca agccaatgct ggtgccagta gtaacgcctt caaaaacacc
    58981 ggagagtatt ttgacctgat ccggctcgcg gcgctgggtg gtatagcgcg atgtcccagg
    59041 gcgacgacga tcaaggtcat gttgcaggtc cgcttccgtc agcggaatgc ctggcggaac
    59101 accatcgacg atgcagccga gcgccagccc gtgcgattcg ccgaaggtgg ttacgcgaaa
    59161 gagttgtcca attgtgtttc cagccatcac ggctccgtta ttgttgtgtt tgcgtgttta
    59221 cttaatcttt ataaatcgtg aaatgttctc gtgcggcaat aagctgctct ttagtgagca
    59281 taaacacacc atcgccgccg ttatcaaact ccagccaggt gaacggaaca tcgggatatt
    59341 gttccataag atgtaccatg ctgttgccca cttcacaaat caacacgcca tcatcagcaa
    59401 ggtaatctgc cgcgttaccg agaatgcgac gcgtcagttt caggccgtcg gtgccagatg
    59461 ccaggcccag ttccggctcg tgacggtatt cgtttggcag gtcggacata tcttccgcgt
    59521 cgacatacgg cgggttagtg acaatcagat cgtattgcac tttcggcaaa tcacggaaca
    59581 gatcggaacg aatcggaatg acgttgtgga tcagaccgtg ttcttcgatg ttctgttcgg
    59641 caaccgccag cgcgtcggga gagatatcta ccgcgtcgac ttccgcttcc gggaaggcat
    59701 aagcacaggc aatggcgatg cagccgctac cggtacacat atctaaaata tgctgcggtt
    59761 gcttgctgat gagtccggca aatttattgt tgatcagttc accaatcggc gagcgcggca
    59821 ccagcacgcg ttcatcgacg taaaattcat gaccgcagaa ccaggctttg ttggtcaggt
    59881 aagccaccgg aatgcgctcg ttgacgcggc ggatcacgcg ttcaacaata cggtgttttt
    59941 cgctggaggt cagacgcgcg gtgcgcatat cttccgggat atccagcggc aggtagagcg
    60001 atggtaacac cagctgtacg gcttcatccc acgggttatc ggtgccgtga ccgtaccaga
    60061 tatttgccgc gctgaagcgg cttaccgacc agcgcaacat gtcctgaatg gtttgcagct
    60121 catttactgc ttcatcaacg aaaattttat ccacgtattc ctccagggca tgatcgcaat
    60181 aatttcggcg gctagtttgc catgaagatg acgataaatc agcattcacg cgcggtgagt
    60241 gaggaaaaat acgtttaaaa cgatcgattg tgctacgagt cgggtaaact gtaggaaaat
    60301 tagaaataga gacagataaa tgaaaaagaa aacaacactt agcgaggagg accaggctct
    60361 gtttcgccag ttgatggcgg ggactcgcaa gattaagcag gacacgattg tccaccgacc
    60421 gcagcgtaaa aaaatcagcg aagtgccggt gaaacgcttg atccaggagc aggctgatgc
    60481 cagccattat ttctccgatg agtttcagcc gttattaaat accgaaggtc cggtgaaata
    60541 tgttcgcccg gatgtcagcc attttgaggc gaagaaactg cgccgcggcg attattcacc
    60601 ggaattgttt ttggatttac acggtctgac gcagctgcag gccaagcagg aactgggcgc
    60661 gttgattgcc gcctgtcgcc gcgaacatgt gttttgtgcc tgcgtgatgc atgggcacgg
    60721 gaagcatatt ttgaagcaac aaacaccgct gtggctggca caacatccgc atgtgatggc
    60781 atttcaccag gcaccgaaag agtacggcgg tgatgcggct ttgttggtgt tgattgaagt
    60841 ggaagagtgg ttgccgccgg agttgccctg aatgctggca gcaaatgccg gagcagcgta
    60901 aacgccttat ccggcctaca aatatgtgat aattcaataa attgcaattc acttcgtagg
    60961 cctgataaga cgcatcagag caatactacg aacttcagtt cattcaatac atactgaaaa
    61021 cgacacgtcg aatcaatcgg ttgcccacta ctggtattca cattcaccac aatatcgtgt
    61081 agtactttat ccgttaccgg atgacgagtt ggcgtaaccg ttaccgttga tttatcacaa
    61141 ccgtaagagg taaaggttaa caattctgat accgcgcggc agtatgctcc tctgccatca
    61201 ttatcagaaa aattactgcc atcacagagt ctgccgcctt ccatcaggta ggtcgaaagc
    61261 gtggtggtca ctgttgaccc cgtggactga attcgtaacg ccttattatg gctgacataa
    61321 ttttcactcc aggagatatc gtatgaagcg accggtatcg ttgggcatat tgtgttatgc
    61381 actgtagggg ccgtatagct tccaaatccg ggtaaatctg tataggaata aaaggatgcg
    61441 atccaagggc aaccataatc atcagatgca acggtaattt taaacgtcat atatgcacaa
    61501 cgaaaaattt tcgtatctat gccagggcaa tttgagttat taattgtatc aaagtcagtc
    61561 agtaccaaag ttggagctga acccgaccca gaccaggaaa taacccgcaa cgtatcgcct
    61621 ttatacgcaa aagaatcata tttcccatat gcaacctcct ttggcgttat caccgtaaca
    61681 ttgaaaacac tgctcgctac agcaccggtt accttgtcca tggcataata aactctcaca
    61741 ctgtcaaaac tcgcactcat aagtttatct ccagcataac tcttagatgc gccgacaaca
    61801 ctcagaacga gcagcattag gctcgcgaaa attatggtcc attttttcat ttcagaaata
    61861 ctcaaaggtt gccgtcactg tggcactaaa gtctccggac gttacatctc gcccgctctt
    61921 tgcctgtaac caggtattaa aatttaggct gttatttccc tgattcagcg ggagcgtgac
    61981 gcctgttgta ttgttgattg gcaccgatgt gcctgccgca gtttcaatac caatcccagc
    62041 gccggatgct gatgacgagg agtcaaaggc cagaaagccg ggtagagcac tatcttctgt
    62101 tcctgtcaac gttactttga cattaaacaa tgtagaacta tgacaatctt tcaattgaaa
    62161 caccaatggc actcgctctg actgcccaag gttcattaaa tcactggcgg caatggcagg
    62221 aaaatgtact tctgccaact ggctaccatt aatcactggc gtacacgatt tactgattaa
    62281 attgccggta aagtgcaaat tatctcctgc tattgtggtt ccactgccca gcaacatcag
    62341 gctactaata aaaaacaacg tacgcttttt cttcattggt aatctacctg taacgtggcc
    62401 caggcctcaa agtccgcctc tggcagaatg acaccactct tttttactgg cacagccgtt
    62461 aacaccggta aatcggtttc attcaccaca agtggcgtat tgatcgtaaa actctgccct
    62521 gcctgtttga gctgaatgcc taaaccagtg atattgctgc ttactgcagc attatcaaaa
    62581 tcgcttggtg tgccactcca gctcaaggtc atttccatta atgggtcgcg aaccgccgta
    62641 tcgcaggtaa tggaatacgg cacggtttgc tcataattgc tgccattaat gtcatcaata
    62701 agcacatcct gaaaagttat ttcaatggtt tgagcgttgc taatcgtgca gttaggcggc
    62761 tgcactaaat aaccgtgaaa agagacgttt gagagtgcca tcgagcaaaa gccccacagc
    62821 aaaataagcg agatccgttt cattggtaat ccaccacaat tgtggcactg gcgttaaatt
    62881 cagctgccgc taacgttgta ccactctgtt tcaccggcac agcttccaga acgggaactc
    62941 ccgaggaaaa attaaatggc aaccatgagc cagaggttaa gtcgagtatt gtgtgatcac
    63001 tacttttctg aacccgaata ccaaacccag gaatacccgt gctgataact gtttcaccgt
    63061 taatcacgac cgttgtcgcc tgcatctgta gacgcaggtc atccattgcc agattgttac
    63121 aaacaagcga gtagtcaatg ggacgtttat aatcaaccct attgatctta ttaatgaata
    63181 cattaccaaa ctcaactgcc gcaccattta ccgtacaagg aggaggggca tcgacaagca
    63241 cccgtaaatt catacttact gacttactcg ctgcaactgc aaagccgcta ccaacacaac
    63301 atatcagtaa cccagttacc gcccagacag aacaagatcc tttcatactg gctctcccgg
    63361 cctcagcctt tgcggctctg ctcttcatcg acctagcaac tgttaccgtt gcaagtgaaa
    63421 aagagcggca tccgagcgcc atagtcgttg acataggtca gtaccggcac gctacccatc
    63481 ttcacattca gcggtgcaga ggttttcggc gacatcacta ttggcgagaa accggctgcc
    63541 ggattgccat ttttctgtgt gctggcatta ctgaaaatga cgtaatacga cgtcgggtta
    63601 ttgacggtaa attcctgacc gttgcgggtt aaagtaatct tatattgcta ggggtttttc
    63661 atgctgacgt tctccagcgc cttcggtcgc cagaacaact taatacgcgt ttgtagtgct
    63721 atttgcagcg tgttggcttt gtttgatttt ggcgggatct cacgcacgtt gaagtagaac
    63781 aggctttcac gatcggcagg caatttgttg atatccggca ttccctgcac ttttacctgc
    63841 ccgttcatca ttgaatcgat gcgttgcacc ggtggcagaa cagttaacgg tgaactgatc
    63901 ttgttaccct tttcatcttc gatccagctt tgggccagat aaggcagttt cggatcgtta
    63961 ttgcgcaacg tcacgctgat tgatttgtcg ctttcattaa atatcaaacg ggtacgatct
    64021 ggcgttacag aagcctgggc agacaaactg gcagcgctaa gcagtaacgc tagcgtcgta
    64081 gcacccaatt tggcagagca aagtaaatca gacatggatc attccttaat cacgttattg
    64141 gttagctgaa acagaaacag ggggattggt gggtgtcacc tgttgcgtct gctcctgaat
    64201 gacaggcttt atgtcatcag gaacaaccgg cgcgacgttg cctttatgct ggcatggcag
    64261 taacaagccg ttaaacagat cggcaggcag cgggtccggc aggttgatat cgcaatgcgc
    64321 aacaccactc cagaacacac tcatgtgttc gccaggtttc actccagcca gataaacatt
    64381 gccgtcatca tcgacaaggc ccactgtctg ctcgttatca ttttttactt ctgcgccaaa
    64441 cggaggatgg ctgccgtctt gtaaacgcag caccgccatc gctttttgac cactgatgac
    64501 ggcaaatttg cggtagccaa tcgccccttc agttagcgtg gcttgtacca ccgactgggt
    64561 tgcttcggcg ttttccggca gtttgttgag gtcgatatac gcctgattgc ggtaatagtt
    64621 attgacgtca gaaacgacgg ctttaccaaa catattggta taaacagcag caccgttacc
    64681 ttctaccgga acatcggcaa cgccatcggc atcaatcaac aagcgtgtcc cgcccatatt
    64741 ctgggtacgg tgaagtgcgc caccgtgggc agtaagcgtt gcgccgccct gtaacgacaa
    64801 gcccgcagag gtgtattgcc cttcatggta gttcgcactg aggtcaacct gcgccagcga
    64861 accatcatgg ctgtaatagc cgtcaacgct ggtgtgtttg tcactggtgc cgacgttcaa
    64921 ctgatagtga gtcgcgtcat cgacacggct gaaataaccg acctgactgc tgtccgtccc
    64981 actgccatag taccgttata gctaacggta ctgttgtcgc cccacggcat actgagcgaa
    65041 atgtacatgc ctttgtcggc ctggttgtca tactcgtaac ggtagccagt catcgagatg
    65101 ctgacattac gaatactgcc catattgaaa tagtgcgaga gcatgatgtt gtagtttgtc
    65161 tgctcctcgc gatcccagta ggtatggcgg gtatagttga gataaaccga aacacccgca
    65221 tcgcggaagt tctggttata agtggcggtg tacatctctt tgtcgttgcc cgtgcgtacc
    65281 attccgctgt cactggcatc cagatactcg ctcatggtca taaagttctc ttccgagaag
    65341 cgatatccag caaaagtaac gcggctgttg agctggtcaa agtctttgga ataactcaca
    65401 cggaaggagt taccgtccag cgaacctttg ccataagcgg tgtctttatc cagtttggtg
    65461 tgcgagtgag taacatcaaa cgcgaccgcg ccgaatgtag acaaatcgcg accgacacca
    65521 agcgccgcag actgatagtt ttcatctccc agtgcgccgc catataacga ccagccgtta
    65581 gcgatccccc aggaagcttc agcaccagaa aaaaattcac cctcgacatg gtgtccccac
    65641 tcttgcggac ggcccatcat gatcttatag cgaacctgac ctggacgagt gaggtatggc
    65701 atcgaggcgg tgctgatgtc atattcctgc acctggccgt tctgttcttc aatgcgaata
    65761 tgcaacgtac cggagacaga atcaccaaga tcctgaatac gaaacggtcc agccggaacc
    65821 tgcgtttcgt aaatcacacg ccccatctgg ctgacggtca cttttgctgt ggtgtgtgcc
    65881 acgccggaaa tgtctggcgc gtagccgcgc agattgggag gcaacatttg atcgtcagta
    65941 ctgacactgc caccaacata gttaaaacca tcaaaaatat cggatctgag gtaatcctcg
    66001 cccagcgcca gtttggcttt taatgatggt aacgcccgcc tggcatagta gcgactccac
    66061 tcccattttt ttgagtttca tcgccgctga attcatcgtc atcattactg cgagtatgtt
    66121 gatagttagt ctgccagtca gcacgcatac gccacggccc taggttaacc ccaaccgtcc
    66181 cgttgccgct gatctcgtta ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt
    66241 tgatgctgta gtccgcgacg atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc
    66301 agtcgggata agtatattcg aggtaagcct gtggcagtga aatgactaat gcggactggc
    66361 ttaaatcagc cttaatttcc atgccttcca gttgaccgga tttcaggcat ttagcatcgt
    66421 ggctccattg cagatttttc gccacgtctt cttttaaacc aaactgcgct accagttccg
    66481 gtgtcagaca agcatagctt ttgctcgcgt catcttcacc agcataccag taaatatcgt
    66541 actcttccgc caatggctgt ttatttagtt gaacctgtaa attgtatttt ccgggctcaa
    66601 catatccctg gctggaaaaa cgcttcatat caatttttgt gtcgcctttt aattccagaa
    66661 aacgggaatc aaactgaatg tcgtcttcag cccagacact gctataactc cctgacattg
    66721 ccagcgcaat gcaccacgga agaatccgta atcgaaaaag agaatggtca ggcatacacg
    66781 tttctatccg tttttattct tgttcggtca aaatacattt aaatccccca tagcagtgtc
    66841 gctatggggg tatgccttac aggtaagtaa tctggaaggt ggtatttgct tcaaaattac
    66901 ccagatctgg cgcatcagct gcgccgacca gccaggcgtt aaagttcagt gtctgcttcg
    66961 ctttgccttt gttggtagaa gtgtctttca ctattttctg gtcaataccc atgccgcttt
    67021 tgtaagaggt acccagagag tcaccgatcg ccaggctaac attgttaaat gccgcaccgg
    67081 tatcggtatt gaaaatggtg taataattgc cgctatttgc agaagaaacg gtaccagtaa
    67141 aggtagaggt cattgtttcc tgagtatcaa atacgcaatc ttgcagacga atctggaaac
    67201 ttttcggcgt ggtggtgccg ttatttttca gtttttcggc accgatatca cccatatcaa
    67261 cttccagttt gtgatcgtcc ggaacgatag aacacactga gtcttcaatg gtgccgtaaa
    67321 aacgtgcagt accgttgtta cccgcagcga ttgttgccgt agaggtgaac acgcccatca
    67381 ccattgccgc agcaatagct gttttaacaa acttactcat tatttgtcct taaactataa
    67441 ataattaaaa atataaacat tgcaatacat tgattcagtc aatagccaat gtttataccc
    67501 agccattgaa tacggcatgc gacccaaaaa gaacaggtcg tagaaaattg tattcattgg
    67561 tgtggggccg catagtgatg tttccacacc cctgatccaa tttaatttta ctaaagagta
    67621 aatgtagatt taattaatat attgacagaa gaaaggtta
//
LOCUS       DAJTOW010000008        66493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTOW010000008 DAJTOW010000000
VERSION     DAJTOW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.008"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO244_000597"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO244_000597"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5662535.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO244_000598"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO244_000598"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5662536.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO244_000599"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO244_000599"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5662537.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO244_000600"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO244_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5662538.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO244_000601"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO244_000601"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5662539.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO244_000602"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO244_000602"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5662540.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OO244_000603"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OO244_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5662541.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO244_000604"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO244_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5662542.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO244_000605"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO244_000605"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5662543.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO244_000606"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO244_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5662544.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO244_000607"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO244_000607"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5662545.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OO244_000608"
     CDS             complement(15666..16703)
                     /locus_tag="OO244_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5662546.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="OO244_000609"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="OO244_000609"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5662547.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO244_000610"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO244_000610"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5662548.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="OO244_000611"
     CDS             19445..19885
                     /locus_tag="OO244_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5662549.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO244_000612"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO244_000612"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5662550.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="OO244_000613"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="OO244_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO244_000614"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO244_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5662551.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO244_000615"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO244_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5662552.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSVQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO244_000616"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO244_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5662553.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO244_000617"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO244_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5662554.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO244_000618"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO244_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5662555.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO244_000619"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO244_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5662556.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO244_000620"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO244_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5662557.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO244_000621"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO244_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5662558.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="OO244_000622"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="OO244_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5662559.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO244_000623"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO244_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5662560.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO244_000624"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO244_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5662561.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO244_000625"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO244_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5662562.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO244_000626"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO244_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5662563.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO244_000627"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO244_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5662564.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="OO244_000628"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="OO244_000628"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5662565.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="OO244_000629"
     CDS             37413..37682
                     /locus_tag="OO244_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5662566.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="OO244_000630"
     CDS             complement(37685..38044)
                     /locus_tag="OO244_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5662567.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="OO244_000631"
     CDS             38185..38811
                     /locus_tag="OO244_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5662568.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="OO244_000632"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="OO244_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5662569.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO244_000633"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO244_000633"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5662570.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="OO244_000634"
     CDS             41651..42316
                     /locus_tag="OO244_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5662571.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="OO244_000635"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="OO244_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5662572.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="OO244_000636"
     CDS             complement(42950..44167)
                     /locus_tag="OO244_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5662573.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="OO244_000637"
     CDS             44301..45350
                     /locus_tag="OO244_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5662574.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="OO244_000638"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="OO244_000638"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5662575.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="OO244_000639"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="OO244_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5662576.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="OO244_000640"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="OO244_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5662577.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="OO244_000641"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="OO244_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5662578.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="OO244_000642"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="OO244_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5662579.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="OO244_000643"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="OO244_000643"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5662580.1"
                     /translation="MTLLNVCGLSHHYAHGGFGGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="OO244_000644"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="OO244_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5662581.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="OO244_000645"
     CDS             complement(51545..51904)
                     /locus_tag="OO244_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5662582.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="OO244_000646"
     CDS             complement(51901..52176)
                     /locus_tag="OO244_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5662583.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="OO244_000647"
     CDS             complement(52235..53359)
                     /locus_tag="OO244_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5662584.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO244_000648"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO244_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5662585.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="OO244_000649"
     CDS             complement(56091..57158)
                     /locus_tag="OO244_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5662586.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57524..59146)
                     /locus_tag="OO244_000650"
     CDS             complement(57524..59146)
                     /locus_tag="OO244_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5662587.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..60997)
                     /locus_tag="OO244_000651"
     CDS             complement(59408..60997)
                     /locus_tag="OO244_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5662588.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61382..62413
                     /locus_tag="OO244_000652"
                     /pseudo
     CDS             61382..62413
                     /locus_tag="OO244_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62500..62976)
                     /locus_tag="OO244_000653"
     CDS             complement(62500..62976)
                     /locus_tag="OO244_000653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662589.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62969..63733)
                     /locus_tag="OO244_000654"
     CDS             complement(62969..63733)
                     /locus_tag="OO244_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5662590.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63979..64740
                     /locus_tag="OO244_000655"
     CDS             63979..64740
                     /locus_tag="OO244_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5662591.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64847..65347)
                     /locus_tag="OO244_000656"
     CDS             complement(64847..65347)
                     /locus_tag="OO244_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662592.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65498..>66493)
                     /locus_tag="OO244_000657"
     CDS             complement(65498..>66493)
                     /locus_tag="OO244_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5662593.1"
                     /translation="NFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTS
                     HKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVA
                     TNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLT
                     FGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKN
                     YDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMA
                     GIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16151 a        17853 c        16321 g        16168 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgtc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaacg gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgaa cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgctc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agattgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcaggttaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctggcgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggggcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actgaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gttcgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cgcgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc acgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtgtcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttggcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg attactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcact tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggccgga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgagcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ttttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaatat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct tcatttctta
    59821 ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa acatcgagcg
    59881 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt cgtgcagtat
    59941 agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt ttattaatac
    60001 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    60061 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    60121 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
    60181 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtctt
    60241 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
    60301 aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
    60361 tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt ttagaaaaca
    60421 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
    60481 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt atgatagaaa
    60541 gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata aaataataac
    60601 catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca tggttattgt
    60661 ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta ttatattctt
    60721 gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga agactatgtt
    60781 catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca aaatcgttat
    60841 ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga tgtgttttat
    60901 caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct ttatggtttg
    60961 aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga tatttttgac
    61021 ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga tataaaacaa
    61081 taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt actaaataat
    61141 ggaaaacaat ccatccctgg aagacaattg taaaaaaaaa caacagaata agaattaatc
    61201 ttaggataaa tatttattta tcatggcctt ttgaccgcta aaaaatggca ctcggcacag
    61261 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
    61321 caggctgaaa ttatcattaa tgcacatttc aatattagca gggatacctg aggagtttaa
    61381 agtgaacata tatatcgggt agcttttcaa attaatccct ttgattatag gcttaatttg
    61441 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa cgtcacgggt catgtgctga
    61501 tttctctggc ggccatatgc ctggcattat tcactaccgc atttattatc atttcgcaac
    61561 tcacgcgcgg cgttaatacg ttttacaata cattgttccc cattattggc tatgcggggt
    61621 caattatcac catgatatgg ggttgggcac tgttagcagg taatgatgtg atggcagacg
    61681 agtttgttgc cggccatgtt atttttggcg ttggtatgat tgccgcatgt gtatcgacgg
    61741 tggcagcgtc gtccggtcac tttctgctca ttcccaaaaa tgcagcgggg agcaagagcg
    61801 acggaacacc ggtacaggct tattcttcat taatcggtaa ttgcctcatt gccgttcccg
    61861 ttttactcac cctgctcggt ttcatttggt ctattacgct gttacatagt gccgacataa
    61921 ctccgcatta tgtcgccttg ggttaaccgc aatctgtgcc tgtctaattg gcctagtggc
    61981 cacaatcgtc catcaaacac gtaatacgtt ctcatccaaa gagcactggc tgtggtgtta
    62041 ttgggttatt tttctcggct cagtcacggt gctgcagggg atatacgtct tagtcagttc
    62101 cgatgcaagc gcccgactgg ctcccggcat tattctcatt tgcctcggaa tgatctgtta
    62161 cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct gttcgttagc
    62221 caacagaata ccgatgatac cggtatttac ctgtctgttt tgcttgtttt tgtcatcatt
    62281 cctcgcggaa atcgcacaaa ctgagctgga ctactttatt ccatctcggg ttctggttgg
    62341 attaggcgca gtatgtttta cattgttctc tattgtttca atattagagg ccggttctgc
    62401 gaaaaaaaat taaaatttac ataatcccag cgttattcat atcttaacgc tggtttttta
    62461 cgaacttagt gaaataatgt agaatataaa attataattt tatattggag cacatgtgtt
    62521 aatgatcaac cccgggatac taaaaatata gacattatta aacttgctta gcctcgcatt
    62581 gcctttacct gttaaagatg catcgagaaa attgagtctg aagttctcat cctttatctg
    62641 attttccagt cccgaaactt tggccttagc attacctgag atccgtccgt tactatctat
    62701 tttgtaggta aaaaaatacg aatgggaaaa tttcctcggt gtatcttcat cagttaagcc
    62761 aacaatcgta aaactaccgc taccatcttt ttttaaaatg actgaaaggt tagcagagta
    62821 gtataaagaa tgatctgttt tagcatttct tgttaacgtc gacgtacatt caagagacgt
    62881 tgtgctacca tcacgagcct tccagagagc aaaaaaacac acctgtagca atgtgaaaac
    62941 aagtattaat ataaaataca ttttcttatt agtcatataa tagcacctct gaacaaattg
    63001 cataagtttc gtcattagag gattttatac atcttgataa ataccgctgc ccttccccaa
    63061 gcaaataatg catactattt acatcatata taaaatttgc acctggcaca cagggtaaat
    63121 gctttttcga aatttcagcg gcctctattt gagatttagc gaggccaatc tcgttaagat
    63181 tctctgaaag atataaatca caggtgtcaa tcttacctga tttaaccaat ggataatccg
    63241 ttatactgta ggtatcttta aaagccacgg caaacatttc aataataaca attactacca
    63301 caagcgaaat caaaatgcga aaaagaggaa tactcctttt gcgctctact ttttcaacaa
    63361 catcttcttg taaatgagaa tcatcataag attgtttact tattgttatg ttttcactga
    63421 tgtaaaagcc tactttgggt atcgtaagaa tcacattttc ttcaacacca atatttttaa
    63481 aacttttgcg taacagggat atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa
    63541 atctctccaa aacgctatca atgattgagt tcctgtcaac cgctccacca ttactttcca
    63601 gtaacactgc caaaatacac cccgccgtag ctggaaaatt tatctcatcc tgggtattta
    63661 cattcgagag cgagcatttg ttcggatcaa aaataatcgc gtcattgata atgaaaaaac
    63721 atctccgctc catgcaggca acaacacaaa ataagatttt gtcattttag cctattcctg
    63781 gtggaactga cacaagaaac taaaatacaa atataaccct gaaaaagtca tataaaactt
    63841 atatccacag aataaaaaat caaacatgtc agttttatca atataaagta tcgcaaggcg
    63901 aaaagtgatg caagaatact cctaagtaaa tgaaagcgca ccacatgaaa ttatcaacac
    63961 atgtaatttc atcattaggt gctatcgaaa ataactcagg agctacttaa gatgaataat
    64021 gtcttactca tgagacccga tgactcagaa tttatgatac ctgatgactg gtcagggcta
    64081 aacgcaaaag gcattacata cgaacaagat aatagagtga cagagtcttt tctgcctttt
    64141 aaatcttttt attgttcacc ggtgtgcacg acagttgaac tcatctcaca aaataattgt
    64201 tttgctatgg gtatccgcta ccttctggaa aagaaaagag acttaaggct aaatcacttt
    64261 aaagccttct ctcataaatg cgagacaaaa cctgacgtgt taattatgac cgggtgcacc
    64321 gtcgctgata cttatagtat attctcacgc attgttgaag aaaatataat gaaaacgatg
    64381 acacctatcg tcatcattac tgggcatcac tcagaagatc tttgttctct ggcatcgaaa
    64441 ctgggaggag ttaaaataat atcaccgaca gaaaagattg caaaaataga aaacgctatc
    64501 aatgatgctt ttaacagcaa gcctaaaaaa ataagatttt taacagatat actctctcct
    64561 caacaaattg agaccatgaa aatggttgcg cagggtagat ccgttaatga gatttcagcc
    64621 gcaatgaata ttagcatcaa gaccgtgtat gcacataaag ttcgtgctat caataagctg
    64681 ggaattaaga aaaaagttta tgaagcgctt ttttataaag cgctaattca gttcaactga
    64741 tgtaaaaatc accctcgctt aataaataat atattctatg tcttttttta taagttaaaa
    64801 aaggcgggat atcaaatccc gccatgaaat taactaatca tctgcattat tctgcgtaga
    64861 tataagcctg ggtacggatg gtgtaagagt ctggctcaac aacctgttct ttcatatggg
    64921 aaacgacgcg ataggcacca cctgttccag tatttttcag catcatataa cgcttaccgc
    64981 caatctcttc ggaggcagct gaaggaggat cctgctcaag cgttgtcagg gaaactttca
    65041 gtttatggtc cgggttattg tcaccagaaa ttactgcatc cgctgaagta ctgctctccg
    65101 cataaatatc atccgggaaa gttacagcat agtcgtggct ctgcccatca gatgcgccaa
    65161 caatacctgt tgccaaaggc gtccatgcag ttaacgtacc gctatatacc cctttcactg
    65221 gttgaatggt attattaaca acgataggct tggtcaaagt aagattagct gacgcttcag
    65281 taccctggct tactaaagtt cccgcctgag aagcaaaagc tgtccccgcc agcatgattg
    65341 ctaacaatgt tttcttcatt atattttctc catgattttc atttacactg caaaatgcag
    65401 tttctttctt tgcatttacg tgctatcgcg aaaatgcttc caccaaataa tggaaacatc
    65461 ttcacaaaaa taaattatga gtttcttaat ttagaaacta taaacatgtc gccgctatct
    65521 ggtataaccc tgatgcactt ttcttcgcag gaagttggta ttttgcgata caggattgat
    65581 ctttagaata accccatttc acattaaact cgccttccgc actcaagcca gaaacataag
    65641 caataccatc ttcacctaca atgccggcca tattttcagt gtctttgcta taactaatca
    65701 cagcgccgaa ggggatgggt ttaccctctt tctgttttaa ggttactaat cctttatccc
    65761 cgctcttcac tgccaatgtc gcaggcacaa cggctccagc tgtcggtaca accaattgac
    65821 tagtttgtgg taagtcgtaa ttcttaccta tcgttttttc atccagaata acatcgtctc
    65881 ttcggtatgg ctgcactggc agtattgcat acccacgaaa atcagtactc agtcccggcc
    65941 agccattaac gttaaccccc gttgctccgg gtgcctctat aagggctaat gtcccatcca
    66001 cattttgacc aaatgtaagt ccatcccttg taataagtat cccaccgctt aaatttgcac
    66061 ctagctgttt catgttttcg ctggagctgt aatggctact aaactgacca tatgatccat
    66121 ttttgccaac ttgcacaatg ttcatcgtgc tcttcggttg tgactgatga gcgttagtta
    66181 tatttgtcgc tacatcccac catacaccac cactattctg acctgataat gaaacctgat
    66241 agttattacc ctgctctccc gaacgactca tctgtgaaga cgcataaatt tgattttcgt
    66301 cacccagcga taatggcact gaaataccca cgcttaattt ccgatctact ttccatttat
    66361 gcgatgtcag caagtcgttt tttgcccagt taatggagaa tgttatgcct ctccataccg
    66421 tactataact caggctggta gaccgcgtgt ttcctgtgcc ccgggaagaa ttatacctcg
    66481 tggtattgaa gtt
//
LOCUS       DAJTOW010000009        66022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTOW010000009 DAJTOW010000000
VERSION     DAJTOW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.009"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(118..597)
                     /gene="folK"
                     /locus_tag="OO244_000658"
     CDS             complement(118..597)
                     /gene="folK"
                     /locus_tag="OO244_000658"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5662594.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(594..2012)
                     /gene="pcnB"
                     /locus_tag="OO244_000659"
     CDS             complement(594..2012)
                     /gene="pcnB"
                     /locus_tag="OO244_000659"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5662595.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2051..2977)
                     /gene="gluQRS"
                     /locus_tag="OO244_000660"
     CDS             complement(2051..2977)
                     /gene="gluQRS"
                     /locus_tag="OO244_000660"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5662596.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3014..3469)
                     /gene="dksA"
                     /locus_tag="OO244_000661"
     CDS             complement(3014..3469)
                     /gene="dksA"
                     /locus_tag="OO244_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5662597.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3647..4351)
                     /gene="sfsA"
                     /locus_tag="OO244_000662"
     CDS             complement(3647..4351)
                     /gene="sfsA"
                     /locus_tag="OO244_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5662598.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4366..4896)
                     /gene="thpR"
                     /locus_tag="OO244_000663"
     CDS             complement(4366..4896)
                     /gene="thpR"
                     /locus_tag="OO244_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5662599.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4970..7399
                     /gene="hrpB"
                     /locus_tag="OO244_000664"
     CDS             4970..7399
                     /gene="hrpB"
                     /locus_tag="OO244_000664"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5662600.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7595..10129
                     /gene="mrcB"
                     /locus_tag="OO244_000665"
     CDS             7595..10129
                     /gene="mrcB"
                     /locus_tag="OO244_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5662601.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10349..12592
                     /gene="fhuA"
                     /locus_tag="OO244_000666"
     CDS             10349..12592
                     /gene="fhuA"
                     /locus_tag="OO244_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5662602.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNGKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12643..13440
                     /gene="fhuC"
                     /locus_tag="OO244_000667"
     CDS             12643..13440
                     /gene="fhuC"
                     /locus_tag="OO244_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5662603.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13440..14330
                     /gene="fhuD"
                     /locus_tag="OO244_000668"
     CDS             13440..14330
                     /gene="fhuD"
                     /locus_tag="OO244_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5662604.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14327..16309
                     /gene="fhuB"
                     /locus_tag="OO244_000669"
     CDS             14327..16309
                     /gene="fhuB"
                     /locus_tag="OO244_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5662605.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16344..17624)
                     /gene="hemL"
                     /locus_tag="OO244_000670"
     CDS             complement(16344..17624)
                     /gene="hemL"
                     /locus_tag="OO244_000670"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5662606.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17849..19270
                     /gene="clcA"
                     /locus_tag="OO244_000671"
     CDS             17849..19270
                     /gene="clcA"
                     /locus_tag="OO244_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5662607.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19294..19359
                     /locus_tag="OO244_000672"
     CDS             19294..19359
                     /locus_tag="OO244_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662608.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19352..19696
                     /gene="erpA"
                     /locus_tag="OO244_000673"
     CDS             19352..19696
                     /gene="erpA"
                     /locus_tag="OO244_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5662609.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19743..20366)
                     /locus_tag="OO244_000674"
     CDS             complement(19743..20366)
                     /locus_tag="OO244_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5662610.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20404..21204)
                     /gene="btuF"
                     /locus_tag="OO244_000675"
     CDS             complement(20404..21204)
                     /gene="btuF"
                     /locus_tag="OO244_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5662611.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLKVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21197..21895)
                     /gene="mtnN"
                     /locus_tag="OO244_000676"
     CDS             complement(21197..21895)
                     /gene="mtnN"
                     /locus_tag="OO244_000676"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5662612.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21979..23496
                     /gene="dgt"
                     /locus_tag="OO244_000677"
     CDS             21979..23496
                     /gene="dgt"
                     /locus_tag="OO244_000677"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5662613.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGMFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23626..25050
                     /gene="degP"
                     /locus_tag="OO244_000678"
     CDS             23626..25050
                     /gene="degP"
                     /locus_tag="OO244_000678"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5662614.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25205..26361
                     /gene="cdaR"
                     /locus_tag="OO244_000679"
                     /pseudo
     CDS             25205..26361
                     /gene="cdaR"
                     /locus_tag="OO244_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26450..26836)
                     /locus_tag="OO244_000680"
     CDS             complement(26450..26836)
                     /locus_tag="OO244_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5662615.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27150..27974)
                     /gene="dapD"
                     /locus_tag="OO244_000681"
     CDS             complement(27150..27974)
                     /gene="dapD"
                     /locus_tag="OO244_000681"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5662616.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28005..30677)
                     /gene="glnD"
                     /locus_tag="OO244_000682"
     CDS             complement(28005..30677)
                     /gene="glnD"
                     /locus_tag="OO244_000682"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5662617.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30739..31533)
                     /gene="map"
                     /locus_tag="OO244_000683"
     CDS             complement(30739..31533)
                     /gene="map"
                     /locus_tag="OO244_000683"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5662618.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAEIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31901..32626
                     /gene="rpsB"
                     /locus_tag="OO244_000684"
     CDS             31901..32626
                     /gene="rpsB"
                     /locus_tag="OO244_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5662619.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32884..33735
                     /gene="tsf"
                     /locus_tag="OO244_000685"
     CDS             32884..33735
                     /gene="tsf"
                     /locus_tag="OO244_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5662620.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33882..34607
                     /gene="pyrH"
                     /locus_tag="OO244_000686"
     CDS             33882..34607
                     /gene="pyrH"
                     /locus_tag="OO244_000686"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5662621.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34899..35456
                     /gene="frr"
                     /locus_tag="OO244_000687"
     CDS             34899..35456
                     /gene="frr"
                     /locus_tag="OO244_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5662622.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35548..36744
                     /gene="ispC"
                     /locus_tag="OO244_000688"
     CDS             35548..36744
                     /gene="ispC"
                     /locus_tag="OO244_000688"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5662623.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36933..37691
                     /gene="ispU"
                     /locus_tag="OO244_000689"
                     /gene_synonym="uppS"
     CDS             36933..37691
                     /gene="ispU"
                     /locus_tag="OO244_000689"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5662624.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37704..38561
                     /gene="cdsA"
                     /locus_tag="OO244_000690"
     CDS             37704..38561
                     /gene="cdsA"
                     /locus_tag="OO244_000690"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5662625.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38573..39925
                     /gene="rseP"
                     /locus_tag="OO244_000691"
     CDS             38573..39925
                     /gene="rseP"
                     /locus_tag="OO244_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5662626.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39955..42387
                     /gene="bamA"
                     /locus_tag="OO244_000692"
     CDS             39955..42387
                     /gene="bamA"
                     /locus_tag="OO244_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5662627.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42509..42994
                     /gene="skp"
                     /locus_tag="OO244_000693"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42509..42994
                     /gene="skp"
                     /locus_tag="OO244_000693"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5662628.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42998..44023
                     /gene="lpxD"
                     /locus_tag="OO244_000694"
     CDS             42998..44023
                     /gene="lpxD"
                     /locus_tag="OO244_000694"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5662629.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44128..44583
                     /gene="fabZ"
                     /locus_tag="OO244_000695"
     CDS             44128..44583
                     /gene="fabZ"
                     /locus_tag="OO244_000695"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5662630.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44587..45375
                     /gene="lpxA"
                     /locus_tag="OO244_000696"
     CDS             44587..45375
                     /gene="lpxA"
                     /locus_tag="OO244_000696"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5662631.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45375..46523
                     /gene="lpxB"
                     /locus_tag="OO244_000697"
     CDS             45375..46523
                     /gene="lpxB"
                     /locus_tag="OO244_000697"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5662632.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46520..47116
                     /gene="rnhB"
                     /locus_tag="OO244_000698"
     CDS             46520..47116
                     /gene="rnhB"
                     /locus_tag="OO244_000698"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5662633.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47153..50635
                     /gene="dnaE"
                     /locus_tag="OO244_000699"
     CDS             47153..50635
                     /gene="dnaE"
                     /locus_tag="OO244_000699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5662634.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50648..51607
                     /gene="accA"
                     /locus_tag="OO244_000700"
     CDS             50648..51607
                     /gene="accA"
                     /locus_tag="OO244_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5662635.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51706..53847
                     /gene="ldcC"
                     /locus_tag="OO244_000701"
     CDS             51706..53847
                     /gene="ldcC"
                     /locus_tag="OO244_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5662636.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     SREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53904..54293
                     /locus_tag="OO244_000702"
     CDS             53904..54293
                     /locus_tag="OO244_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5662637.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54358..55656
                     /gene="tilS"
                     /locus_tag="OO244_000703"
     CDS             54358..55656
                     /gene="tilS"
                     /locus_tag="OO244_000703"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5662638.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKALGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55705..55965)
                     /gene="rof"
                     /locus_tag="OO244_000704"
     CDS             complement(55705..55965)
                     /gene="rof"
                     /locus_tag="OO244_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5662639.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55952..56152)
                     /locus_tag="OO244_000705"
     CDS             complement(55952..56152)
                     /locus_tag="OO244_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5662640.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56318..56863
                     /locus_tag="OO244_000706"
     CDS             56318..56863
                     /locus_tag="OO244_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5662641.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56860..57282
                     /gene="arfB"
                     /locus_tag="OO244_000707"
     CDS             56860..57282
                     /gene="arfB"
                     /locus_tag="OO244_000707"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5662642.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57296..58006
                     /gene="nlpE"
                     /locus_tag="OO244_000708"
                     /gene_synonym="cutF"
     CDS             57296..58006
                     /gene="nlpE"
                     /locus_tag="OO244_000708"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5662643.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58161..58985)
                     /locus_tag="OO244_000709"
     CDS             complement(58161..58985)
                     /locus_tag="OO244_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5662644.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59038..60756)
                     /gene="proS"
                     /locus_tag="OO244_000710"
     CDS             complement(59038..60756)
                     /gene="proS"
                     /locus_tag="OO244_000710"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5662645.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60867..61574)
                     /gene="tsaA"
                     /locus_tag="OO244_000711"
     CDS             complement(60867..61574)
                     /gene="tsaA"
                     /locus_tag="OO244_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5662646.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61571..61975)
                     /gene="rcsF"
                     /locus_tag="OO244_000712"
     CDS             complement(61571..61975)
                     /gene="rcsF"
                     /locus_tag="OO244_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5662647.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62093..62908)
                     /gene="metQ"
                     /locus_tag="OO244_000713"
     CDS             complement(62093..62908)
                     /gene="metQ"
                     /locus_tag="OO244_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5662648.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62948..63601)
                     /gene="metI"
                     /locus_tag="OO244_000714"
     CDS             complement(62948..63601)
                     /gene="metI"
                     /locus_tag="OO244_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5662649.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63594..64625)
                     /gene="metN"
                     /locus_tag="OO244_000715"
     CDS             complement(63594..64625)
                     /gene="metN"
                     /locus_tag="OO244_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5662650.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64813..65388
                     /gene="gmhB"
                     /locus_tag="OO244_000716"
     CDS             64813..65388
                     /gene="gmhB"
                     /locus_tag="OO244_000716"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5662651.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAK"
     gene            65752..>66022
                     /locus_tag="OO244_000717"
     rRNA            65752..>66022
                     /locus_tag="OO244_000717"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15624 a        16344 c        18193 g        15861 t
ORIGIN      
        1 atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa aatttttatt
       61 aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat aaataaatta
      121 ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg tctccccatc
      181 aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc cacgattctt
      241 catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac caaacagcat
      301 gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac gaccctgttg
      361 cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag tttcaagcgc
      421 cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg tgcggtaaaa
      481 cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca gggcagcatt
      541 gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca ctgtcatgtg
      601 gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg cggtgacggt
      661 tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac ctggaactca
      721 ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc tcgcaaggcc
      781 aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc gcgtttaccc
      841 tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt cgtcagacgt
      901 ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc cagcgcgaaa
      961 gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc cagcagtggg
     1021 taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt atggatacgc
     1081 gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc gccattttcc
     1141 gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc acacaacagc
     1201 ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc aaacaggcgt
     1261 gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc ggtttccggg
     1321 ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg taccggatct
     1381 tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt cagatccttc
     1441 ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag gctgttgata
     1501 gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc gcgcagcaac
     1561 atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc gtggtgtcca
     1621 cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag acggaaacgg
     1681 cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt ggcgttagtg
     1741 gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc gccgccaacc
     1801 agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc attttcactg
     1861 atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg tggacgggcg
     1921 actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa attagcgact
     1981 cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa aaatagcggc
     2041 taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc gactcgggca
     2101 cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg ctgaaatcct
     2161 gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt accgggcgtg
     2221 gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg ccttgtggat
     2281 taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc tggtacagcg
     2341 ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct gtaacgccct
     2401 ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca cggcgatgaa
     2461 tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc agcaggtcag
     2521 taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct ggtccatgat
     2581 gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc gcacgcgtac
     2641 aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca cgataggcgt
     2701 cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa tgttccagct
     2761 ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata tcttctatgc
     2821 gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc gcggcgatca
     2881 gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata tactgtgtgt
     2941 ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg taaacgtgat
     3001 ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc gttttgcagt
     3061 cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt tcaacaccgc
     3121 aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc tcgatctttt
     3181 tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct tcttcctggg
     3241 ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt gtaacggtgc
     3301 gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg aagtgcgcca
     3361 gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc accccagcga
     3421 tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg ttgcttctcc
     3481 ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat aaatagcaga
     3541 tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga ggaaaagcgt
     3601 atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac aatgtaaccg
     3661 gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa gccagaattt
     3721 ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg atgtggcgcg
     3781 cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc gcacgctggc
     3841 cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca gtgaccgcat
     3901 cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca atatagcagt
     3961 ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag ccgtatttta
     4021 cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta aggatagcct
     4081 ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg ctctggcttt
     4141 gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc gaataccaga
     4201 cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag tgtagcgtta
     4261 attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc tgaattagcg
     4321 tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt gcgttagcgc
     4381 ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg cgtaaagggt
     4441 gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca ccgcctcgct
     4501 ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa aacaaccgct
     4561 gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg gaggctgacg
     4621 catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa gcgtgagtgt
     4681 gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt tctctgcgct
     4741 cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg gacgtcccgc
     4801 ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt ctgcaggtaa
     4861 gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt tatgaattac
     4921 cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg tgtcgtcgtt
     4981 gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc cgcaggtatt
     5041 attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac tgctggcgca
     5101 tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg cgcgtaacgt
     5161 cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg gctaccggat
     5221 gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg aaggcgtgct
     5281 gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga tccttgatga
     5341 gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg atgtgcaaca
     5401 aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca atgaccgctt
     5461 gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt ttccggttga
     5521 acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg cggtagccac
     5581 cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg gcgtcggaga
     5641 aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg tgctctgccc
     5701 gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg caccgcaagg
     5761 gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca tcgaaggtat
     5821 tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc gcacggggct
     5881 tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg ccgggcgcgc
     5941 cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag cagaacgcgc
     6001 cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc tgatggaatt
     6061 actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac cgccaacggt
     6121 gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg gtgaacggct
     6181 tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag cggcaatgct
     6241 ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg ccattctcga
     6301 agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca atcaaccagc
     6361 ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg gtgaggcaga
     6421 cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg ctcatcgccg
     6481 tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg atgccgacga
     6541 cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca gcgcctcgcc
     6601 ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac aacgctgtcc
     6661 gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta cgctgaaagc
     6721 ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg cgaaaccgtc
     6781 agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt taagcgtgct
     6841 caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg caaagtggtt
     6901 gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc tggaaacgtg
     6961 gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac tcgacattta
     7021 tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata gtgaattgcc
     7081 tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg aagataaccc
     7141 gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc cgacgatcgc
     7201 ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc cgctgcaaat
     7261 cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa aggagatgaa
     7321 agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac cgacgcgacg
     7381 gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag ggccggggtg
     7441 agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg agaggggacc
     7501 gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga acagaaaatc
     7561 gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc gcgagccaat
     7621 tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc gtcgttacga
     7681 agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga tgccgcgcaa
     7741 aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc tatggctact
     7801 gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt atctcgatca
     7861 aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag tttatggccg
     7921 aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga agctgctgga
     7981 ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta ccgtgcaggc
     8041 caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag aagggcaggt
     8101 gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata tggagaacaa
     8161 ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct cctcgccaaa
     8221 cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg tggatacttt
     8281 gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct actccattgg
     8341 gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga gtacgctgac
     8401 gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc gtaaagcgaa
     8461 cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta ttcttgagct
     8521 gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg gcttcccgct
     8581 ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc agcaggcgct
     8641 gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc cgaaactggc
     8701 gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta ttgatcaaga
     8761 actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg gggtgatctc
     8821 tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac tgggcgataa
     8881 ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg cccaggacgc
     8941 ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta agttgagcga
     9001 tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg cgatggtcgg
     9061 aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc gttcgattgg
     9121 ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa tctatcgcct
     9181 gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc aggtctggtc
     9241 accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg tggatgcgtt
     9301 gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc tgcctgcggt
     9361 tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg ttccggcaat
     9421 gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc agaccatcgc
     9481 cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg aagatggtaa
     9541 ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg cggcgtatct
     9601 gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc ttggggcgaa
     9661 atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg tagatacctg
     9721 gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg ataacaacca
     9781 gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt atctggctaa
     9841 ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata tgggcgtgga
     9901 ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct ggaccagcga
     9961 tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca atccgtttga
    10021 tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag agcagaaaga
    10081 cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac atctaagcgt
    10141 gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag atattaaccc
    10201 tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg taccgcttgc
    10261 gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc gtttacgtta
    10321 ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc agccaaaaca
    10381 ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt ctgtttatgc
    10441 acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac ctgcgccgca
    10501 agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta ccggcactaa
    10561 aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg ccgaagagat
    10621 ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg gtgtctctgt
    10681 tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt ttgcggcaga
    10741 aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact tctataacga
    10801 tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc cggtttccgt
    10861 gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc gtccgaccac
    10921 cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc agactggttt
    10981 tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga ccggtcttgc
    11041 gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta ttgcaccggc
    11101 gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact tccagaacga
    11161 gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc cgctgccgaa
    11221 cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct attctcgtaa
    11281 tgggaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta ctgtgcgtca
    11341 gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt acggcgtctg
    11401 ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag cggataaagg
    11461 ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact tctccgttga
    11521 tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc tgaccggtgt
    11581 cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg actctgtgcc
    11641 actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca aagatccggc
    11701 aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt atgttcagga
    11761 tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact gggcagatca
    11821 agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac agtttacctg
    11881 gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca gctatagcga
    11941 atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac cgtctaaagg
    12001 taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg tagttactgg
    12061 tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg gttccttctt
    12121 ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga aagcggcgct
    12181 gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat acaccaccga
    12241 tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt cgctgtgggc
    12301 tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg gtggtcgtta
    12361 tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt atacggtcgt
    12421 ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca acgtggcgct
    12481 gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca cttatggctg
    12541 cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct aatttctctt
    12601 ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga atacacgaat
    12661 cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt
    12721 ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat tggtcacaac
    12781 ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc gtcagaaggg
    12841 gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt tgcccgcaaa
    12901 gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg tgaactggtg
    12961 gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc agatcgcgaa
    13021 aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat
    13081 agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc gcaggatagc
    13141 cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca ggttgatgtg
    13201 ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc cgtgttgcac
    13261 gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag tggtgaaatg
    13321 attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat gatttatggc
    13381 atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt tgtttattga
    13441 tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg ctttctccgt
    13501 tgttatggca gatgaatacc gcccacgcgg cggctattga tcccaatcgt attgtggcgc
    13561 tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg
    13621 ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca gtgattgacg
    13681 tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg tcgtttatgg
    13741 tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg ccgggtcgcg
    13801 gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg ctgacggaaa
    13861 tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa tacgaagact
    13921 ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta ctgctgacga
    13981 cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc caggaaattc
    14041 tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg ggcagtaccg
    14101 ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt tttgatcacg
    14161 acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc atgccgtttg
    14221 tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca acgctttcgg
    14281 caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga gtaaacgaat
    14341 tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg cgctcacctg
    14401 gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct ggtcgccgga
    14461 tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc tggcgatttc
    14521 gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag tactgcgtaa
    14581 cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg ggattaccgt
    14641 caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc tggcaggggc
    14701 ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt cgccggtaac
    14761 gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc agttactggt
    14821 tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa cgctgacgca
    14881 aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg tgatgctgac
    14941 gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg cgcgcaatct
    15001 cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg tcatcagtgc
    15061 gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc cgctgctggc
    15121 aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt tgattggtgc
    15181 gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt ggatggaagt
    15241 gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc tgctgccgcg
    15301 tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga ctgaacgcca
    15361 acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg tggtggtggc
    15421 gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt tgctcgagga
    15481 tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg tcatgctggc
    15541 ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc cggaagtgct
    15601 ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg taccgggtaa
    15661 tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc tgttgatcat
    15721 tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg cggggatggc
    15781 gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg acccgcgaat
    15841 ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg ctcaggtctg
    15901 gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc gccgctggct
    15961 gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc tgacgccgac
    16021 gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga tgactattgg
    16081 accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg gctttcgacg
    16141 gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg tgttcgctga
    16201 ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc tgtcgacctt
    16261 tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat agaatgcctc
    16321 gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc agcatcgatg
    16381 gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc aaacgctgac
    16441 ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa acgctcgacg
    16501 tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa gaagataccg
    16561 aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc cgcttccagc
    16621 agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc cggctgcgcg
    16681 acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga aagcgtaccc
    16741 gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg accaccgaat
    16801 gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc tggcactacg
    16861 gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac ttcatcgatg
    16921 atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa ctctggcagc
    16981 ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc aatctcttgc
    17041 gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca ggttaaggta
    17101 tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt gagtgcgcca
    17161 gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc aaatttaatg
    17221 attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt cgcctcggtg
    17281 ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac cagttgcgcc
    17341 attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc ggcttcaatc
    17401 acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca ggaaccgaca
    17461 taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc tttttcgata
    17521 aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac accgccaggg
    17581 atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg ttcctgattc
    17641 gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat actcgtcaaa
    17701 catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt tttgtgcatt
    17761 tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg ccgtcatctt
    17821 atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt tagaaacacc
    17881 gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc gcgataaaac
    17941 cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc tggctgcggt
    18001 tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc tggtacatac
    18061 tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg tgctggcgat
    18121 gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg ggatcccgga
    18181 aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat tgccggtgaa
    18241 attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg aaggaccaac
    18301 cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc tgaaaggtga
    18361 tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg cggcctttaa
    18421 cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt ttcgctatac
    18481 gttaatttcg attaaagcgg tatttattgg cgtcatcatg tcgaccatta tgtaccggat
    18541 ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc cgcttaatac
    18601 gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta tttttaataa
    18661 atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata ttaccaaatg
    18721 ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg tggcaccagc
    18781 aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt tcagcatggg
    18841 aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct tctcttccgg
    18901 cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg gaaccgcttt
    18961 cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga cgtttgctat
    19021 tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg ggatcattct
    19081 ggttctggag atgaccgata actaccagct cattttgcca atgattatta ccggtcttgg
    19141 cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga ttcttgcgcg
    19201 cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat cagccagcga
    19261 gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg gttagaattt
    19321 gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac tgccgctgga
    19381 gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag ataacccgaa
    19441 tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt atggtttcac
    19501 ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg ttggcctggt
    19561 ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca ccgaaggact
    19621 ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg gttgcggttc
    19681 ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca catcttctct
    19741 cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca acgaatagcc
    19801 gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc cagtggtacg
    19861 gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc agttgcgtag
    19921 atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat gatcccgccg
    19981 ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc cgcattaaag
    20041 gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag caccggcaac
    20101 atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat gctggtgacc
    20161 atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg atccagcgcc
    20221 atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag aacaccaaaa
    20281 gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac cgctgtgccg
    20341 actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg aaaaggtcag
    20401 gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga taatacgtgg
    20461 gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga gctgttcacc
    20521 ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa tagcctgtgg
    20581 cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc tgtctttaaa
    20641 gatgttttct ccgccacaaa ctttgagtac ctggttctga atcgactctt ttccactggt
    20701 aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat cagcatattg
    20761 cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg ccttgtctgg
    20821 ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa tgcttgtcgc
    20881 atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc gctcggcatt
    20941 acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt ccagattcat
    21001 cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt cggaatagct
    21061 gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg cgggagaaag
    21121 cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca gggcgaccag
    21181 cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca accatcaggc
    21241 tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac tgttgatcgg
    21301 ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg cagacatggg
    21361 cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg cggattttcg
    21421 ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca cgtacagcgt
    21481 taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg tcagctttaa
    21541 agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg acatccgcgt
    21601 cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt ggtgccaggc
    21661 caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac agcagagtgg
    21721 cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc tcggttccat
    21781 tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg ttttcgattt
    21841 tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt ttcatagatt
    21901 tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg gaagcgtatt
    21961 tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa ctggcatcgt
    22021 cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat cttcgagagc
    22081 gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa gacccaggtt
    22141 tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat ggaagtccag
    22201 caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct taaattgctg
    22261 gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga gatgtcatgc
    22321 ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc gataaatgac
    22381 tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac tgacgatcgc
    22441 tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga gctgcggcgc
    22501 aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg tctggtgcat
    22561 acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa atatacccgt
    22621 ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa aaagccgggt
    22681 tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa tttggcgctt
    22741 tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc ctattgtgtg
    22801 gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct ttatcatcat
    22861 ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt ggttgaaaat
    22921 gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca gttttttatg
    22981 tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg atttattgat
    23041 aatctgcctg cgattttcgc cggaatgttt aatcatgcat tattggaaga tgccagcgaa
    23101 tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt tagccatccg
    23161 gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt agagatttat
    23221 cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa agaacgggtg
    23281 aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca tcggctggcc
    23341 tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct atgggaatat
    23401 tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct ctatgcgtgg
    23461 gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa agacgaacaa
    23521 taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa acgaatctga
    23581 agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa aaaaaccaca
    23641 ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct ctctgcaacg
    23701 gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc accgatgctc
    23761 gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac cgttaatacg
    23821 ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt ctgccaggaa
    23881 ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa tggcggcggc
    23941 cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga taaaggctat
    24001 gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca gctgagcgat
    24061 ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat cgcgctgatc
    24121 caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga tgcactgcgc
    24181 gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac ggtaacttcc
    24241 gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga aaacttcatc
    24301 cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa cctgaacggc
    24361 gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat cggtatcggt
    24421 tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga atacggccag
    24481 gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact ggcgaaagcg
    24541 atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa ttcctccgct
    24601 gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa gccgatcagc
    24661 agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa actgaccctg
    24721 ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca gagcagccag
    24781 aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat gagcaacaaa
    24841 ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc tgcgcagatc
    24901 ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa aaacatcgct
    24961 gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat tcagcgcggc
    25021 gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg aatacgggaa
    25081 ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca tcatcctttg
    25141 ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc ataacggagg
    25201 acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg cacgtaccat
    25261 gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta tcggcagcgg
    25321 cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt cacagggacg
    25381 agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc aggggattaa
    25441 tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag gtgaaccaga
    25501 gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc tggaacagtc
    25561 gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg tgatgaacct
    25621 gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct ggggatcgat
    25681 ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct tggcgtggac
    25741 agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga gcgtaataat
    25801 ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt gaactctttt
    25861 gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac ccgcatgaaa
    25921 gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg tcctggcagt
    25981 attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca gtggatgcca
    26041 gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga cagtttgcgt
    26101 ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc gatggacaat
    26161 aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca accgctggca
    26221 acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa tcgtatatcg
    26281 gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta tgtggcgtta
    26341 caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg ctcattcccc
    26401 tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat tatttacgcg
    26461 ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact gactccaggt
    26521 gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca cgctgacaga
    26581 tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg acttctttat
    26641 aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat ttaaacttaa
    26701 cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga atatcgttgt
    26761 tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata cccagacttt
    26821 tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct tattttagca
    26881 tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt ccagacttct
    26941 atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc atgggatgga
    27001 ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga tgaaaaagta
    27061 tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga aagcatacgg
    27121 gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag ttcgttaatg
    27181 ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg
    27241 tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga
    27301 ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg
    27361 cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg
    27421 ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc cagcacgccg
    27481 ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg
    27541 gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt aacgtaagac
    27601 ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca
    27661 acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact
    27721 ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag
    27781 agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct
    27841 acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg
    27901 gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc
    27961 tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt
    28021 ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagcgca
    28081 cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt
    28141 cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg cgccagcagt
    28201 ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg ggtcggcaaa
    28261 aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg acggggctgc
    28321 ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat aacctcatgg
    28381 cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata
    28441 ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg
    28501 gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct
    28561 ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa
    28621 tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag
    28681 cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt
    28741 gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt
    28801 cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc
    28861 cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag atagcgcaga
    28921 cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg
    28981 cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt
    29041 gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga
    29101 atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc
    29161 agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt
    29221 tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg
    29281 taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac
    29341 gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc tccggggtga
    29401 cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg ttgttgcaga
    29461 tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg
    29521 ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt
    29581 tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag tggacgtggt
    29641 ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg
    29701 agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg
    29761 ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacaga
    29821 cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc
    29881 agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc gaccatttca
    29941 tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg aatatcgcgc
    30001 aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc atggtaacgc
    30061 tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga aggccagaat
    30121 ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag
    30181 cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa catgcactct
    30241 tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc
    30301 tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg
    30361 atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac caatgccagg
    30421 tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtctatgaac
    30481 tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc
    30541 caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc atcacggggc
    30601 caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt tgcgtactgt
    30661 tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg
    30721 gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt
    30781 gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc
    30841 agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt
    30901 tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca gcacgacgtt
    30961 ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc
    31021 gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt tctgaatctc
    31081 cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca ggtacaggct
    31141 ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga cgataaacat
    31201 tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc
    31261 gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt cattaataga
    31321 gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc
    31381 attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac
    31441 atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc
    31501 gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggttata
    31561 ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt
    31621 ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg acgtctgatg
    31681 ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata aagcgcgccg
    31741 gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa
    31801 caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac
    31861 gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg
    31921 cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat
    31981 gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt
    32041 accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca aaggtaaaat
    32101 ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg
    32161 cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt
    32221 tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga
    32281 caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag
    32341 cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga
    32401 ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat ttgctatcgt
    32461 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat
    32521 ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca
    32581 ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac
    32641 tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac
    32701 atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag
    32761 ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc
    32821 ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt
    32881 agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc
    32941 atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa
    33001 aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac
    33061 ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag
    33121 actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca
    33181 gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt
    33241 gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa
    33301 ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa
    33361 ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa
    33421 ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg
    33481 gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc
    33541 atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc
    33601 aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgtttc
    33661 gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg
    33721 tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat
    33781 cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag
    33841 tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac
    33901 ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct
    33961 tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac
    34021 tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg
    34081 cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga
    34141 acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg
    34201 ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc
    34261 gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact
    34321 cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag
    34381 ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac
    34441 tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc
    34501 tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc
    34561 gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga
    34621 taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt
    34681 attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt
    34741 gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac
    34801 gattacccgt aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg
    34861 tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag
    34921 atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac
    34981 gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc
    35041 cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca
    35101 acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc tccgatctcg
    35161 gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag
    35221 aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag
    35281 tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga
    35341 tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa
    35401 tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc
    35461 ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt
    35521 ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc
    35581 tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt
    35641 gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc
    35701 cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa
    35761 caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt
    35821 gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt acctacgctt
    35881 gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc
    35941 ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc
    36001 gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct gggatacgct
    36061 gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt
    36121 gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg tcatccgaac
    36181 tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa
    36241 tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac
    36301 ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg
    36361 ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac
    36421 tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat
    36481 tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg ccaggcagcg
    36541 acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc
    36601 cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa
    36661 ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt cgccagaaaa
    36721 gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta
    36781 gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg
    36841 tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac
    36901 cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg
    36961 aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct
    37021 gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca aaatccgtcc
    37081 gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta
    37141 gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt
    37201 gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg
    37261 gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa
    37321 cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata
    37381 tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc
    37441 agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc cctgtagatt
    37501 tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg caaattgcct
    37561 atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa gactttgaag
    37621 gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg
    37681 aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct tttgtgttaa
    37741 tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc attgtaacgc
    37801 tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt
    37861 cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt
    37921 tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt
    37981 tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct
    38041 ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct
    38101 ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat
    38161 ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca
    38221 aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct
    38281 ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc
    38341 tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc
    38401 tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt
    38461 taattccagg acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg
    38521 tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag
    38581 ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga
    38641 atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg
    38701 gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg ttatcgccct
    38761 gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga
    38821 actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga ttattgccgc
    38881 aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg
    38941 tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag ctgcggaagc
    39001 acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga
    39061 tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc
    39121 gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga
    39181 gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga
    39241 acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg
    39301 cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct
    39361 tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt
    39421 gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg
    39481 tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc
    39541 gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag
    39601 tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc
    39661 tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct
    39721 tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg
    39781 ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt
    39841 tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact
    39901 tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg
    39961 atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa
    40021 gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggcc
    40081 ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat cagtaatacc
    40141 attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat
    40201 acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac
    40261 aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc
    40321 gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac
    40381 agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc gcgcaaccgt
    40441 gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt
    40501 gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa
    40561 gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgat
    40621 cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa catcgactct
    40681 acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa catcaccgaa
    40741 ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct
    40801 gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac taaagtgacc
    40861 aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta
    40921 cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat
    40981 gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat
    41041 gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag cgatctggtc
    41101 gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc
    41161 caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa agaacgtaac
    41221 actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct
    41281 ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa
    41341 aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac cgtagatggc
    41401 gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc
    41461 gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat taacgaatat
    41521 aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag
    41581 gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat
    41641 aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac
    41701 cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct
    41761 ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat
    41821 gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga tggtttaggc
    41881 ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc
    41941 ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat
    42001 gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg
    42061 gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg
    42121 ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg ggatatgggt
    42181 accgtttggg atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat
    42241 ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg
    42301 ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc
    42361 cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta
    42421 gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg
    42481 gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg caggtcttgg
    42541 tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag
    42601 cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa
    42661 aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaaact
    42721 gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga tggctcagcg
    42781 ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga
    42841 agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca
    42901 ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga
    42961 catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga
    43021 tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt
    43081 tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg
    43141 tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt
    43201 cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca
    43261 aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc
    43321 gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt
    43381 tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat
    43441 cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca
    43501 gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga
    43561 tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga
    43621 gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg
    43681 cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc
    43741 ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg
    43801 cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat
    43861 gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca
    43921 acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa
    43981 gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc
    44041 gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt
    44101 ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa
    44161 gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat
    44221 tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc
    44281 cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca
    44341 caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac
    44401 tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg
    44461 atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct
    44521 ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc
    44581 tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg
    44641 tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt
    44701 ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc
    44761 gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt gaaatatgct
    44821 ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt
    44881 catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg
    44941 atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac
    45001 gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca
    45061 gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc cggtgtggcg
    45121 caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat
    45181 atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg
    45241 tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa
    45301 ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc
    45361 ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc gccggagaaa
    45421 cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt gtgcccaacg
    45481 cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcctggtacg
    45541 aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac
    45601 tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg
    45661 gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca
    45721 aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa
    45781 taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca
    45841 aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc
    45901 cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac tgtctggcat
    45961 tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc ttgaaaactg
    46021 cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg gtgaatgcca
    46081 aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg tcggttcatt
    46141 tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta ctggcgtcgg
    46201 ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga
    46261 agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa
    46321 atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt gagccgcaaa
    46381 aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg
    46441 ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag
    46501 ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg
    46561 tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct
    46621 tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg
    46681 tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga
    46741 accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc
    46801 cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa
    46861 attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag aaatcagtgc
    46921 cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt
    46981 cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc tggaaaaact
    47041 ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca aacgcgcact
    47101 gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga
    47161 accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg gcttggccaa
    47221 aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga
    47281 tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa
    47341 gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg agttaaccca
    47401 cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc tgatctcaaa
    47461 agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga
    47521 attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg gacgcagtct
    47581 tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc
    47641 ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa gctatctgca
    47701 cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta acgatgtgcg
    47761 ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt
    47821 taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga
    47881 agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca ccgttgagat
    47941 cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc agttcccgac
    48001 cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc tggaagagcg
    48061 tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg aatatgacga
    48121 acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat
    48181 cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg
    48241 ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga
    48301 atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga
    48361 cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg acatgtacgg
    48421 tcgtgatgcg gtatcgcaga tcatcacctt cggtacgatg gcggcgaaag cggtgatccg
    48481 cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct cgaaactgat
    48541 cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga
    48601 aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg
    48661 ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac
    48721 cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc agtttgataa
    48781 aagtgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac
    48841 catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccacc
    48901 gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc tgcaacgctc
    48961 ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct
    49021 gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc ctggtccgtt
    49081 gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag agatctccta
    49141 tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa cctacggcat
    49201 tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt ataccctcgg
    49261 tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga tggctaagca
    49321 gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa
    49381 aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc
    49441 ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat
    49501 ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga
    49561 gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc tttaccattt
    49621 ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga
    49681 aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt
    49741 tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat
    49801 catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg
    49861 tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat
    49921 gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc
    49981 gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt acctgaccgg
    50041 acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa
    50101 agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc
    50161 gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc
    50221 cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga
    50281 aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct
    50341 taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg
    50401 gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca
    50461 gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga
    50521 tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt
    50581 aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag
    50641 gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa
    50701 aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga
    50761 agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct tcgccgatct
    50821 cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata ccctggatta
    50881 cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt atgcagacga
    50941 taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga tcattggtca
    51001 tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga
    51061 aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat
    51121 cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc
    51181 tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg tagtttgtac
    51241 ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa
    51301 tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg
    51361 gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct
    51421 gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa
    51481 cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga
    51541 tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta
    51601 cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt tatcgccacg
    51661 gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa catcattgcc
    51721 attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact ggagtcggcg
    51781 ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt gctgaaattt
    51841 atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta cagtctcgat
    51901 ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt catcaacacc
    51961 cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt ttttgaatat
    52021 gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa cgaatatctt
    52081 gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga gcggaagtac
    52141 accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc ggttggctgt
    52201 ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat ttcggtcacc
    52261 gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga gtacatcgcg
    52321 cggacttttg gcgcggaaca gagttatatc gttaccaacg gaacatcgac gtcgaacaaa
    52381 attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg caactgccat
    52441 aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct gaaaccgacg
    52501 cgtaatgcgt tggggattct gggggggatc ccgagccgtg aatttactcg cgacagcatc
    52561 gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt gatcaccaac
    52621 tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct ggatgtcccg
    52681 tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat ctaccagggt
    52741 aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac gcaatcgacc
    52801 cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg cgagtatgac
    52861 gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag ttatcccatt
    52921 gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa acggctgatt
    52981 aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct gcgggaagag
    53041 tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc cgaatgcagg
    53101 cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga tcatatgttt
    53161 ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg caatatgagt
    53221 gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg gatcgtagta
    53281 gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga taaaaccaaa
    53341 gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct caacctgcgg
    53401 atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg caatatgcgt
    53461 attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct ttccggtttg
    53521 atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca ggcatggcag
    53581 agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg tagagtatcg
    53641 gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg agaaatgctg
    53701 accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt agggcaacat
    53761 taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg cgtttaccgc
    53821 gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg agtaacgtgc
    53881 tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat attgcgatta
    53941 ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt ttcacgctgc
    54001 aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg cttaatgggc
    54061 aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc cgaccggaag
    54121 cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca gtggcgcacc
    54181 ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg caaaaacgct
    54241 tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag taaggcttgt
    54301 catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg caccatcatg
    54361 acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc ctttagcggc
    54421 gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga aaatccgggc
    54481 gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga tgcctgggtt
    54541 acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga acgcgtacaa
    54601 cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta tcaggcattt
    54661 gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga cgatcaatgt
    54721 gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc ggctatggcg
    54781 gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg cacgcggggg
    54841 gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga aagtaatcag
    54901 gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt gcagcagcgt
    54961 tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga acaagagagt
    55021 ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca ggggacgctg
    55081 cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat tcgccgctgg
    55141 ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat ctggcaggaa
    55201 gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt tgaaatccgc
    55261 cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga aaccattgtg
    55321 ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac cgtacagctt
    55381 actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt gcgtttcaaa
    55441 gcgctaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa gaaaatctgg
    55501 caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt ttatggagaa
    55561 acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga aggtgagaat
    55621 ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata agacgcatca
    55681 aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac cgatttcagg
    55741 gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag cggcctcgac
    55801 gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct gcaatttttc
    55861 gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct caagattatc
    55921 gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg ctcaccagta
    55981 agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg tcgtcggcag
    56041 ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat cctaagtctc
    56101 cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc acatgccctc
    56161 ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat aaactctgtt
    56221 tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact gcacgtattc
    56281 cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag cgacaattta
    56341 taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg cctctctgac
    56401 gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc tggcgtggct
    56461 gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg atgagccgga
    56521 agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg ggctgccgga
    56581 tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt ttacctataa
    56641 tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc agtttgccaa
    56701 tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct ttgccgatcg
    56761 taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg atgataagaa
    56821 taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat ttcccgacat
    56881 gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg cgctggcggg
    56941 cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg ggcgtccagc
    57001 ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat cagcagtgat
    57061 ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg cgaagccgct
    57121 ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc ccgacgaccc
    57181 acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca aaaatcaagc
    57241 gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg aatggatggt
    57301 gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga tgggatgtaa
    57361 taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga aaccgatgcc
    57421 gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa tctctctgtt
    57481 cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc gtgaagaacc
    57541 ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg tattaaccga
    57601 cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga tgctcgatcg
    57661 tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac aatccagttt
    57721 acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg cggcgacctt
    57781 cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc tggagcgtag
    57841 ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag aaggtcactt
    57901 tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc ccgatacggc
    57961 gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct gcttaaggca
    58021 gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca tataatgcaa
    58081 cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca tccggcattt
    58141 tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc cttgatataa
    58201 atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa cgcagagaca
    58261 tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg cgccagtgta
    58321 agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt
    58381 ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac gcactgttgg
    58441 cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat aaagggaata
    58501 aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc aaacgcggtg
    58561 atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag cttatcacta
    58621 tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg
    58681 ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa gacgcggatt
    58741 ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt gagatcaggt
    58801 gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa tttgacagcc
    58861 catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct gatatcaacg
    58921 gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc ctttggttta
    58981 tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt tctggcatca
    59041 gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta actgtttctc
    59101 gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt cgcccagcac
    59161 aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg gacgctcttt
    59221 gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca gtttctcagc
    59281 aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca cctggaacgg
    59341 cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa tcgccgcagc
    59401 taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt tacggccatc
    59461 ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct ggaagatgtg
    59521 accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc ttggatcgcc
    59581 agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat cccagttgat
    59641 accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca tcgccgcaac
    59701 ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac ccggaccggc
    59761 tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg caacctgcgg
    59821 cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca gcgcaaccag
    59881 cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt tctcaatcgg
    59941 cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat caaccagcgt
    60001 catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg ccagttcaat
    60061 gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc tctgcgccag
    60121 cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct gtacggcgcg
    60181 gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg catcgtaggt
    60241 ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca ggaattcgcg
    60301 ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct ggatctgata
    60361 gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt cagtgataac
    60421 ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa aacgcagcag
    60481 ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg gctgaaccac
    60541 cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga tgttttcgac
    60601 ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg ccagcttgcg
    60661 gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc
    60721 cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc agttggaagg
    60781 tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc agtgcaaaag
    60841 aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct
    60901 gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata ggttttgccc
    60961 gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc gcggataaac
    61021 agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac ctctgcggta
    61081 aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact ggcatcggga
    61141 agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc atcgaccaga
    61201 tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc tttcagctct
    61261 accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa aacccccatt
    61321 ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc ttccatcgtt
    61381 tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg aacggcgtcg
    61441 gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac cagacctggc
    61501 tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat ttgctcgaac
    61561 tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca gcctgacgat
    61621 agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca ttggctttca
    61681 ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc ggagagtcct
    61741 gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac ggtttgccga
    61801 ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct ttcggttttg
    61861 caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta
    61921 acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca cgcatagtat
    61981 ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa atgacaagac
    62041 gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa aattaccagc
    62101 ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca tcagactgat
    62161 aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc gttacgatca
    62221 ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc ggagtcaggc
    62281 caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg tcgtccagag
    62341 aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg ttctcaacaa
    62401 catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag cccacttttt
    62461 gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca acctgcgaac
    62521 catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc ggataaacaa
    62581 aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga tcaaggtacg
    62641 gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg tttggcagaa
    62701 catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc gcaactttct
    62761 gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta atgtggtttg
    62821 gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg gctcccactg
    62881 ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat ttatgttgtg
    62941 ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac tgaattaaat
    63001 aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg tagccgatgt
    63061 agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca cccatcgcgg
    63121 aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc ggcaacgctt
    63181 ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg cgggaagctt
    63241 caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca ataaacggtg
    63301 ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta ccgacaataa
    63361 cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa cggaaaatgt
    63421 tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc cccggacgcg
    63481 tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat ccggatacga
    63541 aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc ggctcagaca
    63601 taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta
    63661 tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc accggcgtag
    63721 tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc
    63781 agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac gcagtcagta
    63841 aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg cagggtcgac
    63901 tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact taccgtgtcc
    63961 tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg cttcacaacg
    64021 tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat gtctttcagc
    64081 agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc atcacacagc
    64141 aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg
    64201 gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga cagcaattcc
    64261 gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg cagagccacg
    64321 ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat accaatctgg
    64381 cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc atcgaccagc
    64441 acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt actcttaccc
    64501 gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag gctgacgttg
    64561 ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt cgaaagtttt
    64621 atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg cctgaaacgg
    64681 gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa tcaggtttat
    64741 gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg ccttattaag
    64801 gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt
    64861 aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga cggtgttatt
    64921 gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac caaccagtct
    64981 ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga gtggatggac
    65041 tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc gcatcatccg
    65101 caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg
    65161 cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat ggtgggcgat
    65221 aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt
    65281 acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt aaatagcctg
    65341 gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgaaatgatt aaaagatgaa
    65401 cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc tataatgcgc
    65461 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
    65521 aaaatcctga aattcagggt tgagtctgaa ataggaaagt gtgatatacg ccacctcgcg
    65581 acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac aatctgtgtg
    65641 ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc aagtctcaag
    65701 agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt taaattgaag
    65761 agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca agtcgaacgg
    65821 taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa tgtctgggaa
    65881 actgcctgat ggagggggat aactactgga aacggtagct aataccgcat aacgtcgcaa
    65941 gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg ggattagctt
    66001 gttggtgggg taacggctca cc
//
LOCUS       DAJTOW010000010        60412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTOW010000010 DAJTOW010000000
VERSION     DAJTOW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.010"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..316
                     /locus_tag="OO244_000718"
     CDS             <1..316
                     /locus_tag="OO244_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5662652.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OO244_000719"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OO244_000719"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5662653.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OO244_000720"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OO244_000720"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5662654.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO244_000721"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO244_000721"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5662655.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OO244_000722"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OO244_000722"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5662656.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OO244_000723"
     CDS             complement(3720..4949)
                     /locus_tag="OO244_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5662657.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNVPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OO244_000724"
     CDS             complement(5013..5453)
                     /locus_tag="OO244_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5662658.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OO244_000725"
     CDS             complement(5558..6328)
                     /locus_tag="OO244_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5662659.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OO244_000726"
     CDS             complement(6325..7251)
                     /locus_tag="OO244_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5662660.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OO244_000727"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OO244_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5662661.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO244_000728"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO244_000728"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5662662.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLRQPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OO244_000729"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OO244_000729"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5662663.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO244_000730"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO244_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5662664.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHACMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OO244_000731"
     CDS             12958..13305
                     /locus_tag="OO244_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5662665.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OO244_000732"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OO244_000732"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5662666.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OO244_000733"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OO244_000733"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5662667.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OO244_000734"
     CDS             complement(15125..15487)
                     /locus_tag="OO244_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5662668.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO244_000735"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO244_000735"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5662669.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OO244_000736"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OO244_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO244_000737"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO244_000737"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5662670.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO244_000738"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO244_000738"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5662671.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO244_000739"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO244_000739"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5662672.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO244_000740"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO244_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5662673.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="OO244_000741"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="OO244_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5662674.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO244_000742"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO244_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5662675.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO244_000743"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO244_000743"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5662676.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="OO244_000744"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="OO244_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5662677.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="OO244_000745"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="OO244_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5662678.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="OO244_000746"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="OO244_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OO244_000747"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OO244_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5662679.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO244_000748"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO244_000748"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5662680.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OO244_000749"
     CDS             complement(36342..36380)
                     /locus_tag="OO244_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662681.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OO244_000750"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OO244_000750"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5662682.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OO244_000751"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OO244_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5662683.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OO244_000752"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OO244_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5662684.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO244_000753"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO244_000753"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5662685.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO244_000754"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO244_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5662686.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VYKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO244_000755"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO244_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5662687.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNVAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO244_000756"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO244_000756"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5662688.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO244_000757"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO244_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5662689.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO244_000758"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO244_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5662690.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO244_000759"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO244_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5662691.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO244_000760"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO244_000760"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5662692.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO244_000761"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO244_000761"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5662693.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO244_000762"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO244_000762"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5662694.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO244_000763"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO244_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5662695.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO244_000764"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO244_000764"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5662696.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO244_000765"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO244_000765"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5662697.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO244_000766"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO244_000766"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5662698.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO244_000767"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO244_000767"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5662699.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO244_000768"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO244_000768"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5662700.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO244_000769"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO244_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5662701.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO244_000770"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO244_000770"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5662702.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO244_000771"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO244_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5662703.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14844 g        14363 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcacgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttggcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatacaagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaatgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattat
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacttcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aagcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc agacgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggta attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgta
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcgacgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgagcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgctcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt ga
//
LOCUS       DAJTOW010000011        58565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTOW010000011 DAJTOW010000000
VERSION     DAJTOW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.011"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..1101)
                     /locus_tag="OO244_000772"
     CDS             complement(<1..1101)
                     /locus_tag="OO244_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5662704.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO244_000773"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO244_000773"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5662705.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO244_000774"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO244_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5662706.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO244_000775"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO244_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5662707.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO244_000776"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO244_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5662708.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO244_000777"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO244_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5662709.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="OO244_000778"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="OO244_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5662710.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO244_000779"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO244_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5662711.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO244_000780"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO244_000780"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5662712.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO244_000781"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO244_000781"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5662713.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO244_000782"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO244_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5662714.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO244_000783"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO244_000783"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5662715.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="OO244_000784"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="OO244_000784"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5662716.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="OO244_000785"
     CDS             11851..12015
                     /locus_tag="OO244_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662717.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="OO244_000786"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="OO244_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5662718.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="OO244_000787"
     CDS             13524..13769
                     /locus_tag="OO244_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5662719.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="OO244_000788"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="OO244_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5662720.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="OO244_000789"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="OO244_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5662721.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="OO244_000790"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="OO244_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5662722.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="OO244_000791"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="OO244_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5662723.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="OO244_000792"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="OO244_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5662724.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="OO244_000793"
     CDS             19432..19785
                     /locus_tag="OO244_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5662725.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="OO244_000794"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="OO244_000794"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5662726.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="OO244_000795"
     CDS             21085..22212
                     /locus_tag="OO244_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5662727.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="OO244_000796"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="OO244_000796"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5662728.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="OO244_000797"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="OO244_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="OO244_000798"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="OO244_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26571..26744
                     /locus_tag="OO244_000799"
     CDS             26571..26744
                     /locus_tag="OO244_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662729.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="OO244_000800"
     CDS             26834..27583
                     /locus_tag="OO244_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5662730.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="OO244_000801"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="OO244_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5662731.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO244_000802"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO244_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5662732.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO244_000803"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO244_000803"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5662733.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO244_000804"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO244_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5662734.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO244_000805"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO244_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5662735.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO244_000806"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO244_000806"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5662736.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="OO244_000807"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="OO244_000807"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5662737.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO244_000808"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO244_000808"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5662738.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /locus_tag="OO244_000809"
     CDS             complement(35512..35607)
                     /locus_tag="OO244_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662739.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /locus_tag="OO244_000810"
     CDS             complement(35721..35888)
                     /locus_tag="OO244_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662740.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36055)
                     /locus_tag="OO244_000811"
     CDS             complement(35891..36055)
                     /locus_tag="OO244_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5662741.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO244_000812"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO244_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5662742.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO244_000813"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO244_000813"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5662743.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="OO244_000814"
     CDS             40511..40762
                     /locus_tag="OO244_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5662744.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO244_000815"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO244_000815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5662745.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="OO244_000816"
     CDS             42067..42825
                     /locus_tag="OO244_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5662746.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="OO244_000817"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="OO244_000817"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5662747.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO244_000818"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO244_000818"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5662748.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="OO244_000819"
     CDS             complement(44425..45045)
                     /locus_tag="OO244_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5662749.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO244_000820"
     CDS             complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO244_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5662750.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="OO244_000821"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="OO244_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5662751.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="OO244_000822"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="OO244_000822"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5662752.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="OO244_000823"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="OO244_000823"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5662753.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="OO244_000824"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="OO244_000824"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5662754.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="OO244_000825"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="OO244_000825"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5662755.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="OO244_000826"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="OO244_000826"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5662756.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53108..53287
                     /locus_tag="OO244_000827"
     CDS             53108..53287
                     /locus_tag="OO244_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5662757.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53373..53873
                     /locus_tag="OO244_000828"
     CDS             53373..53873
                     /locus_tag="OO244_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5662758.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53919..54425
                     /locus_tag="OO244_000829"
     CDS             53919..54425
                     /locus_tag="OO244_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5662759.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGNTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54485..55123)
                     /gene="ompW"
                     /locus_tag="OO244_000830"
     CDS             complement(54485..55123)
                     /gene="ompW"
                     /locus_tag="OO244_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5662760.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55363..55452
                     /locus_tag="OO244_000831"
                     /pseudo
     CDS             55363..55452
                     /locus_tag="OO244_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55480..56223
                     /locus_tag="OO244_000832"
     CDS             55480..56223
                     /locus_tag="OO244_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5662761.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56253..56792
                     /locus_tag="OO244_000833"
     CDS             56253..56792
                     /locus_tag="OO244_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5662762.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56897..57295
                     /gene="yciA"
                     /locus_tag="OO244_000834"
     CDS             56897..57295
                     /gene="yciA"
                     /locus_tag="OO244_000834"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5662763.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57335..58063)
                     /gene="tonB"
                     /locus_tag="OO244_000835"
     CDS             complement(57335..58063)
                     /gene="tonB"
                     /locus_tag="OO244_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5662764.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58278..>58565
                     /locus_tag="OO244_000836"
     CDS             58278..>58565
                     /locus_tag="OO244_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5662765.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKK"
BASE COUNT        14062 a        15146 c        14639 g        14718 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaatgat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccattttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacaatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttaatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtggcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa cagtccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgtcgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaacgcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 attaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagccacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttt ccctctatct tctcgttgcg cttaatttga ctaattctca ttagcgacta
    53041 attttaatga gtgtcgacac acaacactca tattaatgaa acaatgcaac gcaacgggag
    53101 aaataacatg gccgaacatc gtggtagttc aggaaatttc gccgaagacc gtgagaaggc
    53161 atccgacgca ggccgtaaag gcggtcagca tagcggcggt aattttaaaa atgatccgca
    53221 acgcgcatct gaagcgggta aaaaaggcgg tcaacaaagc ggtggtaata aatcaggcaa
    53281 atcctgattc accgttaatt attaatatca gctgataaag ataattgcat ctgcgggccg
    53341 tatggctcgc agtgatttca ctggagaaaa atatgaatat gaagaccatt gaagatgtat
    53401 ttattcaact gctttcagat acctacagcg cagaaaaaca attaacccgg gcactggcaa
    53461 aactcgcaag agcaacatca aatgaaaaat taagtcaggc ttttcatgcg cacctcgagg
    53521 aaactcatgg acagattgaa cgaattgatc aagttgtgga atcagaatca aatctgaaaa
    53581 ttaagcgcat gaaatgtgtg gcaatggaag gtcttattga agaagctaat gaggtcatcg
    53641 aaagtaccga gaaaaacgaa gtgcgtgatg ccgcactgat tgccgcagca cagaaagtcg
    53701 agcattatga gattgccagt tacgggacat tagcgacgct ggctgaacaa ttaggttacc
    53761 gtaaagcagc gaagcttctg aaagaaaccc tggaagaaga aaaggccacc gacatcaaac
    53821 tgactgatct ggcccttaat aacgtaaata agaaagccga aaataaagcc tgaaatatga
    53881 attttaactt ttagtcattt tataaagagg acattttcat gaatcgtatt gaacattatc
    53941 atgactggtt acgtgacgcc cacgcaatgg aaaagcaagc cgaatctatg cttgaatcca
    54001 tggccagccg tatagataat tatcctgaac tacgcgctcg tattgaacaa catcttagtg
    54061 aaaccaaaaa ccagattgtt caactggaaa ctattcttga tcgtaatgac atttcacgtt
    54121 cagtcattaa agattccatg agtaaaatgg ctgcgcttgg gcagtcaatc ggtggtatat
    54181 tcccttctga tgaaatagtt aaaggctcta ttggcggata tgtcttcgag caatttgaaa
    54241 tcacctgtta cacctcacta ttagcagcag caaaaaatgc cggtaataca gtctcaattc
    54301 cgatcatcga agcgatttta aatgaggaaa agcaaatggc cgactggctg attcagcata
    54361 ttccgcaaac aactgagaaa tttttaattc gttctgaaac tgatggcgta gaagcgaaga
    54421 aataataccc tttataccat gtccttattg accccgtata ttacggggtc gtttttgtgc
    54481 ggaattaaaa acgatatcct gctgagaaca taaacaccca cggatcgagg cgtacgctat
    54541 cgtgttgctg tgcaccgcct agcttataat tggcggtggt atcgatatcc atgtaccaca
    54601 ctgacatgtt aaccaaccag tcacggttaa tcagataatc aacccccacc tgcccggcag
    54661 ctccccagga atctttcaga ctgagatcgg aaagccccgc ctctttgcca tgatcgttaa
    54721 atccattatc aaagaaggtg gtgtagttaa taccggcccc aacgtaagga cggaatttgc
    54781 tgctggcatc accaaaatac cactgcgcca tcagtgttgg cggcagatgg tgaacggttg
    54841 caatatcgcc ggtcgcccgg gtgccgattt tatggcggaa cggcgtcgct gccagtaatt
    54901 ccacaccaat gttgtcggtc gccatataag taaacgtcaa gcccagttga gtgttattgg
    54961 tcacgctgaa tccacccaga cttcctaacg taccaccagc accttctgtt ggacgtacgg
    55021 ttgcagaacc tgcacgcata aaaaattcgc ctgcttcatg cgcaaaggca ctgccagaaa
    55081 gaagagttgt tactgccaaa gccgccactg ttaacttttt catatccgct ccgtcgttat
    55141 ggttataaaa acgtgacgaa tatacctaca ttggagtaac aaatgatcct acatagatca
    55201 cattacatat gataattcac catttattga tctggattaa tttagaagtt gcattgagaa
    55261 tcctggttaa tgtgatttaa atcaattttt aaatattttc tctacgcaaa tattaactcg
    55321 atcgccactt gccgcgcttt gcccacgcct gaaggccagc aattacctcc gctttacaaa
    55381 gatatgcttt tccatacaag acctgatgct gccacccgag gcttaaccag ggtacaattg
    55441 cccgctaatt aacacctgca taaaactcaa ggagagtgca tgtctatcac ggcgcagtcc
    55501 gtataccgtg acaccggaaa tttcttccgt aatcaattta tgaccattct gttggtatcg
    55561 ttgctatgtg cgtttatcac agtggtgtta gggcatgttt tctcacccag tgatgcacag
    55621 cttgcgcagc tcaatgacgg cgtgcccgtt agcggcagta gtgggttgtt cgacctggtt
    55681 cagaatatgt caccggaaca gcaacaaatt ttgctgcagg cttcagcggc gtccactttt
    55741 tcagaattaa tcggtaacgc cattctcgcc ggaggcgtaa tattaattat ccagctggtg
    55801 tctgcgggtc agagagtcag tgcgctgcga gctattggtg ccagtgcgcc gatattgccc
    55861 aagttattta ttctgatttt tctgactacc cttttagtac agattggcat catgctggtg
    55921 gtcgttccgg gaattatcat ggccatttta ctggccctgg caccggtgat gctggtacag
    55981 gacaaaatgg gcatttttgc ctcgatgcgt agcagtatgc ggctgacttg ggcgaatatg
    56041 cgtctggtgg cacccgcagt actgagctgg ttgctggcaa aaacactgtt gctgcttttt
    56101 gcctcttctt ttgccgcatt aaccccggaa attggtgccg tactggcgaa caccttgagc
    56161 aacctgattt ctgccatatt gctcatctat ctgttccgcc tgtatatgtt gattcgccaa
    56221 taaccttaag catttgatta cggaatcgta aaatgaagca gtttcttgat tttttaccgc
    56281 tggttgtctt tttcgcgttt tacaagattt atgacatcta tgcggctact gcagcgctga
    56341 tcgtcgccac ggcgattgtg cttatctata gctgggttcg ctttcgtaag gttgagaaga
    56401 tggccctgat cacttttgtt ctggtggtcg tcttcggtgg cttgacgctg ttcttccaca
    56461 atgatgagtt tattaaatgg aaggttacag tcatttatgc tctgtttgcg ggagccctgt
    56521 tagtcagcca atgggtgatg aaaaagccgc taattcagcg gatgctgagt aaagaactca
    56581 cgctgccgca accggtatgg tcgaagctaa atctggcctg ggctgttttc tttatccttt
    56641 gcggtctggc aaacatctac atcgcattct ggctgccgca aaatatttgg gtcaacttta
    56701 aagtctttgg cctgaccgcc cttaccttaa tcttcacatt gttaagcggt atctatatct
    56761 accgccacat gccgcaggaa gataaatcct aaccagactg ccagtccttc ggggctggcg
    56821 ctgtatctcc tctgttctcc tcaggaaaat catagtagca tcgcgcctgt gattttcctt
    56881 ttaagtcggt tttaccatgt ctacaacaca taacgtccct cagggcgatc ttgttttacg
    56941 tactttagcc atgcccgccg ataccaatgc caatggtgac atctttggtg gttggttaat
    57001 gtcacaaatg gatattggcg gcgctattct ggcaaaagaa attgcccacg gtcgcgtagt
    57061 gactgtgcgg gttgaaggaa tgactttctt acggccggtt gcggtcggcg atgtggtgtg
    57121 ctgctatgca cgctgtgtcc agaaagggac gacatcggtc agcattaata ttgaagtgtg
    57181 ggtgaaaaaa gtagcgtctg aaccaatcgg gcaacgctat aaagcgacag aagcattatt
    57241 taagtatgta gcggttgatc ctgaaggaaa acctcgcgcc ttacctgttg agtaatagtc
    57301 aaaagcctcc ggtcggaggc ttttgacttt ctgcttactg aatttcggtg gtgccgttaa
    57361 ttttaaacag gatattcacc acaatcccac tgcctggctt acccggctca taacgccatc
    57421 tgcgcatcgc atttttcacc tcacgctcaa acatgttcgc aggcttggct gagaggattt
    57481 gtacgttatc cacgcgacca tccggcgtga cgtcaaattt aactttaacc tgcccttcaa
    57541 tgcgcaatgc ctgtgctcgt gccggatact gcggctgatt acggcttaat gcgcgtggtc
    57601 ctgaagccac actggtaacc ggcttgctgg ttgcagccgt tgccgtactt gatgtggggc
    57661 gcgcgggtgc cgtattttca aacggtgatg ccggacgcga ctctacgggt ttgacatcgc
    57721 gttttggctg ctcctgtacc tttttcaccg gcttcggttt tggcttaggt ttcggcttcg
    57781 gcttttcaat gaccaccggt gcttcttttg gcggttcggg gatcggctca ggttccggtt
    57841 ctggctctac caccggctcc ggtggcggct gaacggcttg tggcggttcg agatcagcag
    57901 gcgcaaccat cgtgacagaa attggttgcg caggcgcagg tagttcaata acctgatgta
    57961 ccgaggtata gagcagcccc gccacaacag caccatgaat gcagaccgaa agtaacgtcg
    58021 gccaggggaa gcggcgaggt aaatcaaggg ttattgaagt cataatcatt tcagtagaaa
    58081 aactaggcct cgattttaaa tgcaaatagc aatcatattc aataaggcaa gacgatcagg
    58141 atcagtgaaa atgtcatttt tggcgtaatt acccttactt tatgcatgac cttaacaaag
    58201 acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac aactttcccg
    58261 aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct gatagcctcg
    58321 aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg catgatgaag
    58381 gtcgtttgtt gacagctggt ccaatgccag cggtagacag taacgatcca ggggctgcgg
    58441 gatttactgg ctcaaccgtt attgctgaat ttgaatcgct ggaagcagct caggcctggg
    58501 ccgatgctga cccgtacgta gcggcaggag tctatgaaca cgtttcagtg aaaccattta
    58561 agaaa
//
LOCUS       DAJTOW010000012        58024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTOW010000012 DAJTOW010000000
VERSION     DAJTOW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.012"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(17..271)
                     /locus_tag="OO244_000837"
     CDS             complement(17..271)
                     /locus_tag="OO244_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5662766.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            426..998
                     /gene="yeiP"
                     /locus_tag="OO244_000838"
     CDS             426..998
                     /gene="yeiP"
                     /locus_tag="OO244_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5662767.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1221..2687
                     /locus_tag="OO244_000839"
     CDS             1221..2687
                     /locus_tag="OO244_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5662768.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2805..3791
                     /gene="yeiR"
                     /locus_tag="OO244_000840"
     CDS             2805..3791
                     /gene="yeiR"
                     /locus_tag="OO244_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5662769.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3830..4543
                     /gene="lpxT"
                     /locus_tag="OO244_000841"
     CDS             3830..4543
                     /gene="lpxT"
                     /locus_tag="OO244_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5662770.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4955..5521
                     /gene="mepS"
                     /locus_tag="OO244_000842"
     CDS             4955..5521
                     /gene="mepS"
                     /locus_tag="OO244_000842"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5662771.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5702..7258
                     /locus_tag="OO244_000843"
     CDS             5702..7258
                     /locus_tag="OO244_000843"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5662772.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7340..9154
                     /locus_tag="OO244_000844"
     CDS             7340..9154
                     /locus_tag="OO244_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5662773.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9155..10249
                     /locus_tag="OO244_000845"
     CDS             9155..10249
                     /locus_tag="OO244_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5662774.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10249..11274
                     /locus_tag="OO244_000846"
     CDS             10249..11274
                     /locus_tag="OO244_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5662775.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11276..12865
                     /gene="yejF"
                     /locus_tag="OO244_000847"
     CDS             11276..12865
                     /gene="yejF"
                     /locus_tag="OO244_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5662776.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12869..13213)
                     /locus_tag="OO244_000848"
     CDS             complement(12869..13213)
                     /locus_tag="OO244_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662777.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13546..14736)
                     /gene="bcr"
                     /locus_tag="OO244_000849"
     CDS             complement(13546..14736)
                     /gene="bcr"
                     /locus_tag="OO244_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5662778.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14764..15459)
                     /gene="rsuA"
                     /locus_tag="OO244_000850"
     CDS             complement(14764..15459)
                     /gene="rsuA"
                     /locus_tag="OO244_000850"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5662779.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15609..17369
                     /locus_tag="OO244_000851"
     CDS             15609..17369
                     /locus_tag="OO244_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5662780.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17494..17778
                     /gene="rplY"
                     /locus_tag="OO244_000852"
     CDS             17494..17778
                     /gene="rplY"
                     /locus_tag="OO244_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5662781.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17917..18924)
                     /gene="yejK"
                     /locus_tag="OO244_000853"
     CDS             complement(17917..18924)
                     /gene="yejK"
                     /locus_tag="OO244_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5662782.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19106..19333
                     /locus_tag="OO244_000854"
     CDS             19106..19333
                     /locus_tag="OO244_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5662783.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19353..21113
                     /gene="pbgA"
                     /locus_tag="OO244_000855"
     CDS             19353..21113
                     /gene="pbgA"
                     /locus_tag="OO244_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5662784.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21188..21264
                     /locus_tag="OO244_000856"
     tRNA            21188..21264
                     /locus_tag="OO244_000856"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21222..21224,aa:Pro,seq:ggg)
     gene            complement(21367..23958)
                     /gene="yejO"
                     /locus_tag="OO244_000857"
     CDS             complement(21367..23958)
                     /gene="yejO"
                     /locus_tag="OO244_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5662785.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24278..24925
                     /gene="narP"
                     /locus_tag="OO244_000858"
     CDS             24278..24925
                     /gene="narP"
                     /locus_tag="OO244_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5662786.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25136..26188)
                     /gene="ccmH"
                     /locus_tag="OO244_000859"
     CDS             complement(25136..26188)
                     /gene="ccmH"
                     /locus_tag="OO244_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5662787.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26185..26742)
                     /gene="dsbE"
                     /locus_tag="OO244_000860"
     CDS             complement(26185..26742)
                     /gene="dsbE"
                     /locus_tag="OO244_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5662788.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26739..28682)
                     /gene="ccmF"
                     /locus_tag="OO244_000861"
     CDS             complement(26739..28682)
                     /gene="ccmF"
                     /locus_tag="OO244_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5662789.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28679..29158)
                     /gene="ccmE"
                     /locus_tag="OO244_000862"
     CDS             complement(28679..29158)
                     /gene="ccmE"
                     /locus_tag="OO244_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5662790.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29155..29364)
                     /gene="ccmD"
                     /locus_tag="OO244_000863"
     CDS             complement(29155..29364)
                     /gene="ccmD"
                     /locus_tag="OO244_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5662791.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29361..30098)
                     /gene="ccmC"
                     /locus_tag="OO244_000864"
     CDS             complement(29361..30098)
                     /gene="ccmC"
                     /locus_tag="OO244_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5662792.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30140..30802)
                     /gene="ccmB"
                     /locus_tag="OO244_000865"
     CDS             complement(30140..30802)
                     /gene="ccmB"
                     /locus_tag="OO244_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5662793.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30799..31416)
                     /gene="ccmA"
                     /locus_tag="OO244_000866"
     CDS             complement(30799..31416)
                     /gene="ccmA"
                     /locus_tag="OO244_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5662794.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31435..32037)
                     /gene="napC"
                     /locus_tag="OO244_000867"
     CDS             complement(31435..32037)
                     /gene="napC"
                     /locus_tag="OO244_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5662795.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32047..32496)
                     /gene="napB"
                     /locus_tag="OO244_000868"
     CDS             complement(32047..32496)
                     /gene="napB"
                     /locus_tag="OO244_000868"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5662796.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32493..33356)
                     /gene="napH"
                     /locus_tag="OO244_000869"
     CDS             complement(32493..33356)
                     /gene="napH"
                     /locus_tag="OO244_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5662797.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33343..34038)
                     /gene="napG"
                     /locus_tag="OO244_000870"
     CDS             complement(33343..34038)
                     /gene="napG"
                     /locus_tag="OO244_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5662798.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34045..36531)
                     /gene="napA"
                     /locus_tag="OO244_000871"
     CDS             complement(34045..36531)
                     /gene="napA"
                     /locus_tag="OO244_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5662799.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36528..36791)
                     /gene="napD"
                     /locus_tag="OO244_000872"
     CDS             complement(36528..36791)
                     /gene="napD"
                     /locus_tag="OO244_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5662800.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36781..37275)
                     /gene="napF"
                     /locus_tag="OO244_000873"
     CDS             complement(36781..37275)
                     /gene="napF"
                     /locus_tag="OO244_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5662801.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37683..38171
                     /gene="eco"
                     /locus_tag="OO244_000874"
     CDS             37683..38171
                     /gene="eco"
                     /locus_tag="OO244_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5662802.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38293..39324)
                     /locus_tag="OO244_000875"
     CDS             complement(38293..39324)
                     /locus_tag="OO244_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5662803.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVLHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39558..39917)
                     /locus_tag="OO244_000876"
                     /pseudo
     CDS             complement(39558..39917)
                     /locus_tag="OO244_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40135..41778)
                     /gene="yojI"
                     /locus_tag="OO244_000877"
     CDS             complement(40135..41778)
                     /gene="yojI"
                     /locus_tag="OO244_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5662804.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41854..42504)
                     /gene="alkB"
                     /locus_tag="OO244_000878"
     CDS             complement(41854..42504)
                     /gene="alkB"
                     /locus_tag="OO244_000878"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5662805.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42504..43568)
                     /gene="ada"
                     /locus_tag="OO244_000879"
     CDS             complement(42504..43568)
                     /gene="ada"
                     /locus_tag="OO244_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5662806.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43642..44697)
                     /gene="apbE"
                     /locus_tag="OO244_000880"
     CDS             complement(43642..44697)
                     /gene="apbE"
                     /locus_tag="OO244_000880"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5662807.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44809..45930)
                     /gene="ompC"
                     /locus_tag="OO244_000881"
     CDS             complement(44809..45930)
                     /gene="ompC"
                     /locus_tag="OO244_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5662808.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46668..49340
                     /gene="rcsD"
                     /locus_tag="OO244_000882"
     CDS             46668..49340
                     /gene="rcsD"
                     /locus_tag="OO244_000882"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5662809.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49357..50007
                     /gene="rcsB"
                     /locus_tag="OO244_000883"
     CDS             49357..50007
                     /gene="rcsB"
                     /locus_tag="OO244_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5662810.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50207..53056)
                     /gene="rcsC"
                     /locus_tag="OO244_000884"
     CDS             complement(50207..53056)
                     /gene="rcsC"
                     /locus_tag="OO244_000884"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5662811.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53331..54107)
                     /locus_tag="OO244_000885"
     CDS             complement(53331..54107)
                     /locus_tag="OO244_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5662812.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54112..55761)
                     /locus_tag="OO244_000886"
     CDS             complement(54112..55761)
                     /locus_tag="OO244_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5662813.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55762..56321)
                     /locus_tag="OO244_000887"
                     /pseudo
     CDS             complement(55762..56321)
                     /locus_tag="OO244_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56480..>58024)
                     /locus_tag="OO244_000888"
     CDS             complement(56480..>58024)
                     /locus_tag="OO244_000888"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5662814.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14192 a        15654 c        14260 g        13918 t
ORIGIN      
        1 tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc caggtcatgg
       61 cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca cagactttcg
      121 gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga atgcagggcg
      181 tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa ggggcagtgc
      241 aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt aaccaccgga
      301 ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat accacttgcc
      361 cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac tacaggaatt
      421 tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac aacggcaaac
      481 tgctgctggc taaggatatt gatattcagt cgcccactgc ccgcggtgcc gctacgctgt
      541 acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt ttcaaaggtg
      601 atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc tatgtcgatg
      661 gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc accaaagatc
      721 agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg caggtgctga
      781 cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg gaaatcgttg
      841 aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg gcgacattga
      901 gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa attcgtatcc
      961 atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat gcagaaaagg
     1021 gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt gcgcttcctg
     1081 aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt catcgcatta
     1141 acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg ttaatttatc
     1201 gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg catcatgtac
     1261 acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt ggctttggcg
     1321 cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc cagggcggcg
     1381 actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc cagctccgcg
     1441 cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag gtgataattg
     1501 tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca attattgaga
     1561 aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa ggctattgta
     1621 ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac gatctacaaa
     1681 cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa cgccgccgtg
     1741 agcgcagcct tacaccgttt accgtgctct cctgcgacaa tattcccgac aatggccatg
     1801 tggtgaaaaa cgcggtgctg agaatggcag aaaaacgttc gccagaactc gccgggtgga
     1861 taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg gctgcaaccg
     1921 acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc gcgattagct
     1981 gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt cctgcctggg
     2041 aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg aaactgcgta
     2101 tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga ttcgcctata
     2161 tcagtgattg catgcaggat cgcgtatttc gctatgctgc cagaacatta atgctggatg
     2221 agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg gataagttaa
     2281 ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg atggatggca
     2341 gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg cgtgaaacgg
     2401 actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc ggcgttgatg
     2461 atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc gaacttgttg
     2521 cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa gttttcggtg
     2581 atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg caacaaatcg
     2641 tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga tttctgcggt
     2701 taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc ttttttagcc
     2761 aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc aggaccaacc
     2821 tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg ttagcccata
     2881 aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc ggaattgatg
     2941 gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc tgcatgtgct
     3001 gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag ggaaaaccag
     3061 accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc gatctgttaa
     3121 ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt ctcgatccac
     3181 gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag ctggctgccg
     3241 cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag caagcgctac
     3301 agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag catgggaaag
     3361 ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc gccagcgccg
     3421 cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg ccagagcatc
     3481 aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc tggatattcg
     3541 acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt gcaccggtgg
     3601 aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac cgtcagggcg
     3661 atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt gagctgattt
     3721 ccagcagcga agctgactgg aatgccttgc agagcgcgct gttgaagctt cgtttagcga
     3781 ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata tgattaaaaa
     3841 tttgccgcaa atagtgttgt tgaatattgt cggccccgcg ctgtttcttt cctggtatat
     3901 tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt tctttaatca
     3961 gaaactggtc gaaagtaagg cctttttatg gctggttgca ttgactaaca atcgcgcctt
     4021 cgacggttgt tcactgctgg cgatgggtat gttgatgctg agtttctggc tgaaagaaaa
     4081 cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa ctgcagtggt
     4141 attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa cattgacttt
     4201 taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg cctcacgaga
     4261 tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca tgtggcgtta
     4321 tttcggcaaa gttgcaggcc ttatcgccct tattattttt atggtttttg catttcccag
     4381 agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga ccgtgatatt
     4441 gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca ccttttttga
     4501 caaatcacta ccaggaaaaa acaaacattt ccaaaacaaa taactcacag taattaacat
     4561 catcagggtt atttttatag tgaggataat cctgatgatg cgcaccgtgc tttcatctat
     4621 cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca ctcaattgta
     4681 ctttaattga ccaaccccgc ttattaactt tctgtatcac tttttcttat aaaaaatcat
     4741 gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt tgcctcggcg
     4801 gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg ccttaaggaa
     4861 ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt ataacgatat
     4921 ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac cgattttgag
     4981 atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg catgtagtgc
     5041 aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg aaacatcatc
     5101 actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg taaaatcgcg
     5161 aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg gcagcactaa
     5221 aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat ttggcttaga
     5281 acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc gcagtaattt
     5341 gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg tcggtattta
     5401 tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta tttccagcat
     5461 gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca gccgcagcta
     5521 ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa gcactgctta
     5581 ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc ttgatccagg
     5641 ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa ttaacggaat
     5701 catgttcata cgcgctccca attctggacg taagctcctg cttacctgca ttgtagcagg
     5761 cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc ataagcacga
     5821 agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct attttgccga
     5881 cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc agcaggctaa
     5941 ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg tgctggtgaa
     6001 agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac tcaatgaatt
     6061 gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg gcacgccgat
     6121 ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga aaaatagcgg
     6181 tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt cacggcagga
     6241 agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct tttcttcacg
     6301 tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa ttgcgggcat
     6361 tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt ggtacgcatt
     6421 tttactgggc ggcatgagtg gaactgtcgt tggcctgctt tgctattacc tgatgagcgt
     6481 acgtatgcgc cccgacagag aaatcatgac cgccatcaag cgcgaacaat tttacgtggc
     6541 gtatcaaccg gtggtggata cacaagcttt gcgggtaacg ggcctggaag tactgctacg
     6601 ctggcggcat cctgtagcgg gagaaattcc cccggatgcc ttcattaact ttgccgaatc
     6661 gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc gcgatgccgc
     6721 agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg cgccggacca
     6781 tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc tgcccgcaca
     6841 ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc aagaagccac
     6901 acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg acttcggcac
     6961 cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga aaattgaccg
     7021 tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg acgcggtgct
     7081 gacgctggcg aaacgtctca atatgctgac agttactgaa ggggtcgaaa cgccggaaca
     7141 agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga ttagtcaccc
     7201 gttaccgctg gacgattttg ttcgctggct gaagaaaccg tatacgccgc agtggtaagg
     7261 tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct aatgatgagt
     7321 aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg ctctgttcac
     7381 cttcggtgcg caggcgcagg ctatcaagga aagctatacc tttgccgtgc tgggcgaacc
     7441 ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc caaaaggtgg
     7501 gcagataacg ttgtcagccc tcggtacctt cgataatttc aaccgctatg cactgcgcgg
     7561 caacccgggc gcacgcaccg agcagctgta cgacacgtta tttacgactt ccgatgacga
     7621 accaggcagt tattacccgc tgattgctga aagtgcacgc tatgctgacg attattcctg
     7681 ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttctccca ttactgcccg
     7741 cgatgtagag tttacttttc aaaaatttct gaccgaaggc gtgccgcaat ttcgtctggt
     7801 ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg agttagctaa
     7861 acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag aaaagtactg
     7921 gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg gtccgtacca
     7981 cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag attactgggc
     8041 agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct acgattatta
     8101 cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt tgcgtatgga
     8161 aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata aaaaatacat
     8221 catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg cgtttaatat
     8281 ccaacgtgcg gtattcagcg atcgccgggt gcgggaagca atcactctcg cctttgactt
     8341 tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca gttattttca
     8401 aaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc ttctggcacc
     8461 aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc cggtatctaa
     8521 aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca acgaagcggg
     8581 ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc tcagctttga
     8641 attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc acagcctgca
     8701 acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca ctaaccgcat
     8761 gcgcaatcgc gactatgaca tgatgccgcg actatggcgg gcgatgccgt ggcccagttc
     8821 cgatttacag atttcctggt catcggaata tatcaattcc acttataatg cccccggcgt
     8881 gcaaagcccg gttatcgact cactgatcaa ccaaattatt gccgcgcagg gaaataaaga
     8941 aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt attacatgct
     9001 gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct cccaacccgc
     9061 tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta ataaagcggc
     9121 caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga ttcgccgtct
     9181 gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca tcgtgcaaat
     9241 tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta atgccggagt
     9301 attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg gtgtcggcaa
     9361 tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga tcgctgagat
     9421 cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa tgctctggga
     9481 ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc tgacgctgat
     9541 taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga ttatctatct
     9601 ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct ttgacgtctg
     9661 gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg ccatcctgct
     9721 gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg gcctggtttc
     9781 cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt ggcatatcac
     9841 gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc tgaccaaaaa
     9901 ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga aaggggtaag
     9961 tgagaaaaac attctctgga aacatatgtt ccgcaacgcc atgctgctgg tgattgccgg
    10021 ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg aggtgatgtt
    10081 ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg attatcctgt
    10141 aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata ttgtcagtga
    10201 tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat gtcgcgactc
    10261 agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg ctactggtcg
    10321 ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat cgctaacgat
    10381 aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa aaactacagc
    10441 gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc gtggctgaaa
    10501 caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg tgctaccagt
    10561 atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa ctggctggga
    10621 acggatgcaa acggcggcga tgtgctggca cgcattctct atggcacgcg gatctcggtt
    10681 ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc gggggcgcta
    10741 caaggctatt acggcggtaa agtcgatctc tgggggcaac gctttattga agtatggtcg
    10801 gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc taacttctgg
    10861 tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt ggtgcgggcg
    10921 gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct tggcgtcagc
    10981 gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac cctcaccttt
    11041 ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt cctcggcttc
    11101 ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa aaataacctt
    11161 caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt gtctttgctg
    11221 atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt gtagcatgac
    11281 gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc aaaccgtacg
    11341 tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg cgctggtggg
    11401 tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc tcccttcccc
    11461 gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc ttcatgccag
    11521 cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc aggaaccgat
    11581 ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc tttcactcca
    11641 ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg atcgcgtcgg
    11701 tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg gcggcgaacg
    11761 ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa ttgccgatga
    11821 accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt tgcgcgaact
    11881 gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca ttgtcagaaa
    11941 actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa ataacgccgc
    12001 tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca gtgaaccgtc
    12061 aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg aacagcttca
    12121 ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata atgtggtggt
    12181 gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg gcgagtctgg
    12241 ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc agggcagcat
    12301 cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac ctactcgtca
    12361 tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac tcaacgtttt
    12421 gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg cacaacgcga
    12481 acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac gccaccgtta
    12541 tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg cattaattct
    12601 taagccctcg ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa cggtacaggc
    12661 gcaaatattg acgcttttga aatcattgca acaaaagcat caactggcct atttgtttat
    12721 cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac tgcgacaagg
    12781 ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc aggagtatac
    12841 gcgtcagtta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa aagggttcgg
    12901 caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc tggcgattta
    12961 caaacaggca cggctcacct tcacactcca acaccgaaca tggaacttcg atgtcgctta
    13021 acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg ctaagcaatt
    13081 taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt tcaaccttcg
    13141 aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg attgagagtt
    13201 gtaatgaagt cacttatttt ccccggaagt acattcactt cacaatttgt ttacatcaat
    13261 tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg cctcgcctgc
    13321 gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt tgcggggcta
    13381 tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt gactttttac
    13441 ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt gtggttgaca
    13501 tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc gttttttcgg
    13561 ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa ttgaccaaat
    13621 catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa ttgcccctat
    13681 gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg gaaactcatc
    13741 aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa acgccgcaac
    13801 gccaaccacc agcgaccaga atcccagccc cagcggcgca ctgatgacca tccacgcagc
    13861 cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa tgcggcggac
    13921 gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg caaagtaata
    13981 accaaagttt tccggcgcga cgtggttaat ttcaatataa acaaacggtc cggcgcttaa
    14041 gaatgagaac atcccggcaa agctgaaacc actggcaagc atgtagctca ggacacgttt
    14101 atggcggaac agcgccgcaa agttaccaat agtggtacga atgtgaaatg gctgacgacg
    14161 ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca gaatcgccgc
    14221 taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
    14281 tatcggtgcc atcagcagtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
    14341 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
    14401 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
    14461 acacgccacc gcggcggcgg caaacaccag cgtcccgcca agaaccaccg gcttacgccc
    14521 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
    14581 actgagggtc atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa ttaccggtag
    14641 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
    14701 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
    14761 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
    14821 atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca cgtggttacc
    14881 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
    14941 ctgcgttggg gtaatcactt caagcaccgc cggcttagtg agatcttttt cgttatgcag
    15001 ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt ccagtgtcac
    15061 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
    15121 atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg cgtgcagttt
    15181 ccacgctacc ggttcatcca gaaaatagag caccgtcggg tgatccgggt cgtccgtgga
    15241 gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
    15301 cgggttgcca tcgtaagcga catcatgttc agggagcagt ttgaacgctg cattacgaac
    15361 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
    15421 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
    15481 aaacggaggt cgggtaatga cccgaaaatc tgaactgttg tctgcccagt atagcggtct
    15541 aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata atctacctac
    15601 gcgaaaccat gatttttaca ctccgcccat atcagcaaga agccgtggat gccacgctca
    15661 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc gcaggtaaaa
    15721 gcctggtgat agcggaactg gcacggctgg cgcgtggtcg cgtactggtg ctggcacacg
    15781 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
    15841 tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt ggcagcgttc
    15901 agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
    15961 aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
    16021 ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
    16081 aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag aaagcccttt
    16141 tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc tatctgacgc
    16201 cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg caggcacaga
    16261 gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag caacgcatta
    16321 cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg gtaatgattt
    16381 tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgctg
    16441 cactgattac tggcgacaac cccggcgctg agcgcgatgt gttaattgaa gattttaaag
    16501 cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt gacgccccgc
    16561 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
    16621 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
    16681 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
    16741 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
    16801 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
    16861 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
    16921 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
    16981 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
    17041 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
    17101 atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc cagcgtaccg
    17161 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct ctgcgctgga
    17221 tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat tttgtcgtcg
    17281 cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac gaaggtcgtt
    17341 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
    17401 agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
    17461 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
    17521 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
    17581 atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa agtcatgaac
    17641 atgcaagcta aagctgaatt ctacagcgaa gttctaacca tcgttgttga cggtaaagaa
    17701 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
    17761 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt
    17821 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
    17881 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
    17941 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat cggttgctgg
    18001 atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
    18061 gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga agctctcttc
    18121 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
    18181 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
    18241 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
    18301 gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca atccttcgct
    18361 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
    18421 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
    18481 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
    18541 gcgcatactg ctcaggttgc tcagaactgc caccagcaaa tactccaccg ccagataacg
    18601 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
    18661 caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
    18721 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
    18781 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
    18841 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
    18901 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
    18961 accgccagtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
    19021 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
    19081 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagta atgaacaggt
    19141 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
    19201 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
    19261 ccaacgccag gcaattgcca actcttttgc cagcgcctta cagtcctcta tcaacgaaga
    19321 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
    19381 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
    19441 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
    19501 gcatttattc ctacgtaagc attatcggcc atttcagctt cctagtgttc gccacctact
    19561 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
    19621 ccgtcattct ggcgacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
    19681 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
    19741 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
    19801 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
    19861 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
    19921 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
    19981 acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg caggagtatc
    20041 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgtacagtat ccgttaagcg
    20101 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
    20161 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
    20221 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
    20281 tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
    20341 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
    20401 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
    20461 aatctgacga tcagaccgcc acgcagtgga tcaactggct gggccgctac gcacaagaag
    20521 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
    20581 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaacgcg
    20641 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtggtgatt atcactgccg
    20701 gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac ggtcatctgc
    20761 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gctctgactg
    20821 atcataccga tctgatgacg acgctgatgc aacgcctgct gcatgtcagc acacctgcca
    20881 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
    20941 ctgcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
    21001 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
    21061 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
    21121 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
    21181 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
    21241 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt acggttggtt
    21301 tttttatatc tgcaattaat tcgataaaca gaccgtgaca catcacagcc tgtttatttt
    21361 ctgttatcag aacgtccaga ccacacccgc ctgagtattc cacggcgatt ctacccctgc
    21421 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
    21481 cccagcctga taaacgccac tggttccagc cacatcatta ttaaatttgc catcgtcatt
    21541 aactttcacc tggttagaat cggcgtattc ctgacgcacg gcggctttca gccagggttc
    21601 cagcgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
    21661 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
    21721 atctgtggta aagccggtaa aggccagata gggtctaaca ctccacaatc cgtcaaccca
    21781 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
    21841 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
    21901 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tatagctatc
    21961 gatattacct tttccgccgc gatcaaaacc aatatcagaa tgagagtaac caaagaacaa
    22021 gccgcgaatt gtactgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
    22081 cgtcaatgtt tgctcaaaac cagctcccgc atcagtggtc acgttgttgc gggtgttaat
    22141 tgccgaactc cacatcaccg tatcgtaact aacgcctttt acgctaccaa gacgctcacg
    22201 cacggtgtcc agttctgcat caaataccag cggttgtgcg gctgccatgt tcagcacatc
    22261 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
    22321 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
    22381 aaatgcagca tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg ctgccgggct
    22441 ggcaccggtg tccgtcacga atattttgaa atcacctgtt gcctgaccgg tgacgttgag
    22501 ctgatcgctc tgatgattgg ccatatcggt acgcatataa aaattgccgt ttcccgacag
    22561 ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg aacgagtcac
    22621 tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta agttgaggcg
    22681 agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat tttcgagcga
    22741 aacgtccgct ttgctggtat ccacggcacc atgcgttcta aaagaagcgc cctcagaaac
    22801 cactacctta cccaccaaca tagcgggtgc ataatttatt tgcggcttca tgacctcaag
    22861 aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga cattagcgcg
    22921 gccattgttg attaccatat tttcagagat ggcttttgag gaatacgtga tacttccatt
    22981 ctggtctgat cgaccgagat catataccgc atttgcctgc actttcgtac cagtatcctt
    23041 tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatggcct gcgtgtcctc
    23101 cataacaatg agcccggaac catcatccag ttcataattt tttgacacac catcctttat
    23161 actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg agacaattaa
    23221 tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa cctccagtaa
    23281 gccgccacta tccacagtag tattataagc gaagtcattt tcttcaactc gcaaacttcc
    23341 gccgttttcc aacaacatat tattagcaat accattcttg atatcgaact gaccgagacg
    23401 gtttgttccg aatacctcca tggcccgcgt ggttgtttta atagcaccgc ctgctttctg
    23461 atctaccgca agcgcaaatc ccccctcttt aacctccagt agtcctccct tttcgatagc
    23521 ggcaaaaacg gcccccccat ctttttcaac gattaagcgc ccgcctttaa tcgtggtatc
    23581 attcgctggg ccataaacct gtagcacacc attattgagg gtagtctggt ttgccgcgcc
    23641 acccatttga acaatttgat aaccatcatt taatactgag tattctgcgg taccattcat
    23701 ttcaatgtat tgataccctc cgttaatttc agtattacta cttacaccaa attcttttat
    23761 atgctgttct ccgccaggat tgattttcgt attattggcg gtcccataaa ccagctggat
    23821 atctttttcc aggataacac catcaactgt ctcaccatac tcaactgttg atgccaatgc
    23881 tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac aaagagaaac
    23941 agaaccagat tgatgcattg agctttcatc ctatgaaatt aattgcagtt aaaagcattg
    24001 ggtacagaaa atacccatag ctccataccc ggagtcagtt tttaaaaact gtttaaagaa
    24061 atgcacaagt attgtgattg attttttagt tgtttttctt gatgagaagc tgatgcaaaa
    24121 ttccgtcttt ataataaaaa tgatgccaaa gcgaacgaca aggttgtagt tttcactaca
    24181 tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa cgctacgctc
    24241 aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa cgccttttca
    24301 ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt tactggagct
    24361 tgatcctggc tttgaagtgg tcgccgaagc gggcgaaggc gcgagcgcga tcgatctggc
    24421 gaatagactg gatatcgacg tgatcttgct ggatctcaat atgaaaggta tgagtggcct
    24481 ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta tcctgaccgt
    24541 atccgatgcc tccagcgatg tctttgcgct gatagacgca ggcgcagacg gttatctgtt
    24601 gaaagacagc gacccggaag tattgctgga agcgattcgt accggagcga aaggcagcaa
    24661 agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg gcgcggaaga
    24721 agatcccttc agcgtgctga cggagcgcga gctggatgtt ctgcacgagc tggcacaggg
    24781 gctgtcaaat aaacagattg catcggtgtt gaatatttcc gagcagacag taaaagtaca
    24841 tattcgcaat ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca ccattctgtt
    24901 cctgcaacaa cgcggggcac aataaaaata gcccgatgga tttatcatcg ggctgagatt
    24961 tatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg gatcgatcaa
    25021 tttattgaat ttacacaatt ttgtaggccg gataaggcgt tcacgccgca tccggcataa
    25081 actaagcgca ctttgtcatc agtctagccc gatggcatca ccatcgggcc tctttttatt
    25141 tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca atcaccgcac
    25201 ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca acggcttcgc
    25261 caaatcgctg ctgctcaaag gcattaaacg catacatgct tagcacacgg atattgctat
    25321 ggtccgttct caccagctga cgtagcagtt caccgccgag gcggttgtcg ttgggatcag
    25381 atgaacgagt caacgcttca gcgtatccca gtgcggcatc gctgttcttc ggatcgaggc
    25441 gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc atgccaacgc
    25501 ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga gtacgcatcc
    25561 ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg tccagcgccc
    25621 tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc tgctgataat
    25681 tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata cccggcagat
    25741 aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac gcttccggca
    25801 ccacgcgtac ccgacgccgc gagcgggcgt atatgaccca accgccaatg ccaatagcca
    25861 ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg taagtgacga
    25921 agttgccgta acgcgccacc atataatcga caatctcttt tttactttta ccttcctgca
    25981 tcagttcata cactttctga cgcaggtcgg tggcaatcat cgagttggaa tcggcaatgc
    26041 tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac tgttgttcct
    26101 gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg gagatcatca
    26161 gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt tctcccacag
    26221 cggcttgatc tcttcttccc agacgcgagg attgagatcg cccgcatggc gatagcgaat
    26281 gataccgttg ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga gatccagccc
    26341 taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct ctttcagcca
    26401 gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgaa tgccctgcgc
    26461 tgaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccaag tcgcccagac
    26521 gttaagcagt actggtttgc cctgagtcag cacatccgcc tgataaaact gccccggatt
    26581 gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg attccagatt
    26641 ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg cggcaatcgc
    26701 caggaagata atcaacggaa ttaacaatac tttgcgcttc atacggcctc cggcgcagtt
    26761 ttttgcggac tcacgcgctt acgatagcga ggatcaaaca gacacagcag tccgcccaac
    26821 gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta aagacgcacg
    26881 gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg cgtgatgccg
    26941 ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg tttttccgca
    27001 tacagcaccg tttccggttt accctctcgc gttacgccaa tagtcgccac accgccgcgc
    27061 cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc atgaatatcg
    27121 acgctatcgc cggacttcat gcgcacatca cgctcaacgc tataattctg gctaaaggca
    27181 atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca ataactgaag
    27241 gtggttttcg tgccgcgtga aatacgcagc gcagcttccg cagttgccag caccgcaatc
    27301 cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc gaacagccac
    27361 ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa attgcggatt
    27421 ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga caccaagcag tagcgcaaac
    27481 ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga aatactgccc
    27541 agtccaagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac cagcatcgcg
    27601 gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag cgcattgttt
    27661 acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc gccaatcacc
    27721 agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa cgcgtgtacc
    27781 gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga gaaggcactg
    27841 attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac cgccagtgag
    27901 tgcatcagtg cagtccccac cagccacggc ataaacgagg cgttttctac cggatcccag
    27961 aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag cacaatgccg
    28021 agcgttagga aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc ataagtgctg
    28081 tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga gaaacccacg
    28141 taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa cagcgggtta
    28201 agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt agaggtaaag
    28261 agaatgaaca gcaaaaagcc gacgctgacc atccccatta tcgccagtac acgggccaca
    28321 atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc gctcatcagc
    28381 agcacccaca gcagaagcga accttcatgc gcgccccaag tagccgccac gcgataccac
    28441 accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc gttgaccacg
    28501 aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag ccaggcaaac
    28561 aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag cggatacacg
    28621 gacagcagca gcgcaatccc taacgccaag cacagcagcc cgtttccaat ttccggcatc
    28681 atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc atcgctttct
    28741 caacttctgg cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc gcgaggatat
    28801 gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg aacagatccg
    28861 gcaaaatgcc ttcgtaagag acatccactg agccttcagc atcgtaaatg gtgaaggtca
    28921 ctttcagcga attgggatcg cgctgcacgc tacccggcat caccatcccg ccaacgcgca
    28981 gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga atttcccccg
    29041 gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg atagtcagcg
    29101 ctagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga atattcatgc
    29161 agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt gcgccacacc
    29221 acgcagaatt gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca gcggaataac
    29281 ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga aaaattcatt
    29341 ccaggaagca aatgctgggg tcatttacgg cctcttttca gtatcagttc actcacccac
    29401 ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt cagcgtggca
    29461 gacaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc cggatcgata
    29521 ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac ggagtaatga
    29581 ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg gcccgccaga
    29641 cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa cagcagcacc
    29701 agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg ttttccccat
    29761 gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc catcgccgcc
    29821 accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc caccgccatt
    29881 gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat aatgcggtag
    29941 ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca gccgacggta
    30001 agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat ttgatacagc
    30061 cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc agactcgaac
    30121 aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc gtcgcaaaag
    30181 gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac ccatcaacgg
    30241 gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat gggatagtca
    30301 gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact gtcagcgcca
    30361 cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagcagcgtc agcgccatca
    30421 cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa aggatgagta
    30481 acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg gcgggcaagg
    30541 gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca cggaacagtc
    30601 gttccagcgc cagcaaggat gaaagcagcg cagcaaccca gataatgccc ggtgcaatac
    30661 gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt acaatcagga
    30721 agaaccacag cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc agttcaagac
    30781 ggaaaatacg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc ggcgaatttt
    30841 actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc cctgctccgt
    30901 atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg cggtaaaagg
    30961 ctcgtcgagg atccataatg tggcacgggt caaccacaga cgcgctaaag cgacgcggcg
    31021 ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa gcccggcctg
    31081 cgccaaggct tccagacatt gtgcggcatc gccatcgcga tgataaaagt gcagattttc
    31141 taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca gggtttgatg
    31201 gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc cagcgtcagg
    31261 gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt tgctaccggt
    31321 gatttgtacc cactctcctg cgttcagcgt aaatgacaag ccactaaata aggttcgttc
    31381 atcccgctca caaagtaact ctctggcttc aagcataccc acgcaataac cctgttaaaa
    31441 acctggctcg acttcacgca tatcgggcag cttgtgcgcg atccctttat ggcaatcaat
    31501 acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg atttctgggc
    31561 ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt tattgtcctt
    31621 catgcgccgc cactcattct gtgccatcgt cagacgatga gcttcaaatt tctgcggcgt
    31681 gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct tgcgtatcat
    31741 cttcggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga cgccgctacg
    31801 gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttgcgca tttcgtggca
    31861 gctaatgcag aactcttcgg tattggcttt ttccatcccg gtgttaaagc ccccccagaa
    31921 gacgatgccg ccaacaaaac cgatcaacag cagcgtcccc agcgccagat ggctgggggt
    31981 acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat ttcccataat
    32041 aacctcttat ttcccgtaac cttttgatgg ggtaaaggta ttccccacga ttggcgcggt
    32101 atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca cttccgcgcc
    32161 cactttgccg tcgctgtcca taaagtgagt aggactgata cgcggcgcgc cagtggtgcg
    32221 atagctttcg acaccgtggc actgcaagca gcgattggta ttggtcgtta cctgataacc
    32281 ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca tccgatcctg
    32341 ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg actggctaaa
    32401 atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca tcgccgtcca
    32461 ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga actccatcgt
    32521 gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca cgtcatgcaa
    32581 tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg tagcacatgc
    32641 ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg atcgctggcc
    32701 gcaacggtaa tcacaccttt gctacccagc acgccataca gcgcgccaac cggacaaatg
    32761 tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc gagaataagc
    32821 agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagagaaac ggggttgatc
    32881 cattcccaaa tgagcgtgcc agttaaggct gatcccacca gaatgaccac caacagcacg
    32941 tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaagcgcct gcgtaaccag
    33001 ttcgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa tcgctttccc
    33061 gccagggcgt agagcacggt gataatcacc gcaccggtca acgccaccgt ggcaggcaga
    33121 tgaccactgg caagactttg cagcgtcatc agcggatcgg tcagcggaac ggtgtcgaat
    33181 aacaggctac tgctgtagtt gccgtgcaag atccacacac caaaccatgg accgctcaaa
    33241 aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg gtgactgcgc
    33301 caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt tgccattgtt
    33361 cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca gtgacagcgg
    33421 taacaccttg attgccggtt gttccagcac gcatactttt tcgcacttac cgcaaccggt
    33481 acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac gcgtgttgcg
    33541 ctccagctcc aaggtgatgg cctcatcaat tttcgggcat tcgcgataac aaacatcgca
    33601 gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccgcca gccccatccg
    33661 cgcgtcgtcg atcgattcaa tctcacgatc cagcgcaccg cttgggcaca ctttggcgca
    33721 cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg gcgtgcccgc
    33781 cgacagacca gaggccagcg tcgccagttt taaggtgtcg taagggcaag cctgaacaca
    33841 ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc cgggcgggcg
    33901 caaccgcacg ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca cacccacggc
    33961 agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac cattttgagg
    34021 tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc acttcttgaa
    34081 gtccgtctct ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa ccagctgtgc
    34141 ggcgtcgaag aacggcatgt acaccaggcc ctgtggcgga cggttacgac cgcgcgtttc
    34201 aacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc cacggcgcag
    34261 atcgcgcgct ttcgcatcca gcgggtgaat aaacaagacc gcttccggga aggcgcggtg
    34321 cagttccggc acacggcgag tcatactgcc ggtgtgccag tgctccagaa cgcgtccggt
    34381 agagagccac aggtcgtact cttcatccgg tgcttccgcc gccggttcga acggcagtgc
    34441 gaagatcacc gctttgccat ccggtttacc gtagaactta tagccttcgc ccgctttcac
    34501 gtacgggtcg ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca ccaccggcca
    34561 gcgcagaccg cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac cgtgaccgcg
    34621 accaaaccat gcgtactctt cgaacagccc tttttgcaga tagaagccca gctcgcggga
    34681 ttcatcgttc agctgatctt ccgccagttc ggataccggg aatttgctca cttcgggggt
    34741 ggcatacaga acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg ccagcagttc
    34801 ttccggccat acttcttcag ttttgaagcg gcgggagaac tggactaact gccagagatc
    34861 cgatttcgct tcgcctggcg cctgtacctg ctgacgccag aactgagtac ggcgttcggc
    34921 gttaccgtaa gcgccctctt tctctaccca cattgcggtc ggcaggatca agtcggcggc
    34981 cagcgcactg actgtcggat acggatcgga gacgatgatg aagttgcgcg gatcgcgcca
    35041 gcccggcata cgctcttcat tgatgttcgg cccggcctgc atgttgttgg tacacatggt
    35101 ccagtaaaca ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg catgaagacc
    35161 gattttcgcc ggaatggtgc cgctcgggat attccacttc ttctcacaga tatcacgatg
    35221 tttctcgtta gttaccacca tgtccgcagg cagacggtga gcaaaggtgc ccacttcacg
    35281 cgcagtacca cacgcagaag gttgcccggt cagggagaac ggaccgcaac ccggctggga
    35341 aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttagcccaca caccacgggt
    35401 atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat cggcatacag
    35461 ctgcgccagt tgttctaact ggtctttcgg cacgccggtc atttcagcgg ttttttccag
    35521 cgtatactca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg aggcgtcaga
    35581 acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt agccgatgtc
    35641 cgtcgcccct ttgcgcaggt taacgtgctt gctgaagaag tcctgattta tcgcattgtt
    35701 ttgaatgata tagttggcga tgtagttcag gatcaccagg tcagattgcg gcgtaaagat
    35761 gatgccgtta tccgccagct cgaagctacg atgctggtag gtagaaagca ccgccacggt
    35821 gacgttctgg ttagagagac gactgttagt gatgcgtgac cagaggatcg ggtgcatctc
    35881 cgccatgtta gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt catagcagcc
    35941 catcggctca tccataccaa aggtacgcat aaagccaact actgccgacg ccatacagtg
    36001 acgtgcgttc gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct tggacgcggc
    36061 ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt ccggcccttt
    36121 ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat cccaggtgat
    36181 tggggtaaat tcgccttctt tgtcatattt accgtttttc atacgcagca gcggctgcgt
    36241 caaacggtct ttaccgtaca tgatcttggg caggaaatag cccttaatgc agttcaggcc
    36301 acggttaacc ggtgcgtccg ggtcgccctg acaggccacc acacgcccct gctgcgttcc
    36361 gaccagaacg ccgcaaccag taccgcagaa acggcacggc gctttatccc atttaatggc
    36421 ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg cagccgccgc
    36481 agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca tggtgtttcc
    36541 tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc ctctacgttg
    36601 cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac caccacaatc
    36661 aactgaccgc tcggcgcgtc gctgacagca acttcacagc cgggaaaggc gttcagttgg
    36721 gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca aacttgccag
    36781 ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg ctgcacatgc
    36841 tccacatccg ttgcaaagtt ggctattaag ttgcgcctgg tagatcccgg agagcgtcgg
    36901 gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt caactgactg
    36961 atacgccagg cacgcgtccc cgatggtgaa ctgcaaatcc caggccctgg tgtggcgcgg
    37021 agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc actcgttatt
    37081 tttgaaatta acgctcggat agccacccgt gccgcgttgc agaatgttgt tttcacaagc
    37141 attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat caccggacca
    37201 gggcggacgg ataccgttac tggctttgcg ccagcaacca gtgagtatgc cccgacggga
    37261 tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg agagcgacca
    37321 aatagaccgc ataattaata agccattttt atagccgcta agatattaaa ggatgtgtca
    37381 aagatgcata ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg gcgggaagcg
    37441 tggcaaaaac gggcttttgc tcacatttca aatggttata aacatattta tatagcgatt
    37501 aattcaccag agatatttct gctggtttgc tccctcatca gaatttaaca ctaaaagagc
    37561 aggtaaaatt gtctgaatgt tctttaagtt attcataaag caaattaata aatctgatga
    37621 atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag gaaagcaaaa
    37681 aaatgaagac cattctacct gaagtattgt ttgccgcttt cgctaccact tccgcctggg
    37741 cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct gaaacaggca
    37801 tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg aaagtagaac
    37861 tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc gggaagctgg
    37921 aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa gttagctcgc
    37981 cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt gtcaccgcgt
    38041 atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg gtgtatacgc
    38101 cagacaatgt agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt gacaacgcgg
    38161 aagttcgcta aactgttgtg aagtacggca ccccgttggt tggataaggc ggttacgccg
    38221 catccaacat ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt cttatcagta
    38281 tacgccacat cattacaacg caatatccgc cacttctttt tgaacgggtt gcggtttcaa
    38341 ctgcggtttc ggcgtactat ctgctgcttg cggtttgtcg tatttcagcc ccagcacttc
    38401 gctggtgtac tgcaactcgc gttctgttgc cgctacatca ccgttcagct tgcgtccata
    38461 agacggaacg atcgctttca acgtagcctg ccattgcggg ctggaaacgc gatcgccaaa
    38521 tactttttcc agtagattca acataatcgg cgcggcggtt gacgcccctg gcgatgcccc
    38581 cagcagcgcc gcaatggtcc cttgctggtc actgaccact tcggtaccca gacgcagtac
    38641 gccacctttc tcggcatcac gcttgataat ctgcacacgc tgccccgctt gccacaaacg
    38701 ccagtcctct tttttcgctt gcggatagta ctctttcaac gcttcaaaac gatcctcttc
    38761 actcaacatc acctgactca ccagatattt caccagatcg aaattatcca gcccgacgtg
    38821 catcatcggc atcacgttag aggtggtggt ggaactcatc agatcccaca atgaaccgtt
    38881 tttgaggaat ttggttgaga aggtggcaaa tggcccaaac agcactacgc gtttaccgtc
    38941 cagaacacgg gtatcgatat gcagaaccga catcggtggt gcgccaacgg atgctttacc
    39001 gtaaactttc gccagatggc gattaaccac gtctggattt tccgaaacaa ggaactgtcc
    39061 gcccaccggg aaaccggcgt agtctttcgc ttccggaatc cccgattcct gtaacagctt
    39121 cagcgccgca ccgcccgcgc cgataaagac aaatttggcg cggatgttct gtgcagtgcc
    39181 gtttttcaga tcggcaacgg taacggtcca ggtgttatcg tcattacgct ttagggcgcg
    39241 gacttcgctg ctgagttgca gcgagaagtt agatttcttc tgtaaggaag caattaactg
    39301 gcgggtgatc tcaccatagt tcacatcagt gccaatttct gttgcggatc gcgcccttcc
    39361 atcactaacg gtgcccactc tttgatctgc gcgtgatctt cagagtaacg cataccgcga
    39421 aacagcgagc tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattaac gttatcctcg
    39481 ccccagacaa agctcatatg cggaacggta ttgataaatg aacgcggagt acgcagcacg
    39541 ccgcgctcga cctggtgcgc ccagaactgg cgggaaatct gaaatgcttc gttaatggcg
    39601 actgcttttt caatactgat gctgccatcg gcgttttgcg gggtgtagtt cagttccatc
    39661 agtgcagaat gcccggttcc ggcgttgttc tagccgttcg aactctcctg cgcgcccccc
    39721 tccaggcgct ccaccatggt catcgaccat tcaggctcca gctcgcgtca ataggtcccc
    39781 aacgtggcgc tcataatgcc gccgccaatc aacagtacat cagtttcctg ctcctcggac
    39841 gctttcgctt ttgccgccat cgaaacggca ttaagcccca cggccatcga gaagagcatg
    39901 gcagtcactt ttttcatctt gttaatgcct tacttttagt cgctttattg caggtgagat
    39961 ttgcgcggca tcaacggtaa cacttaagta acaacattta aataatgttt ataaattata
    40021 ttctaatttt agaaacatta taatttcgtt gattaattat agggttatta gaaatgaagg
    40081 gatttttagc atattgacct gacggcagca gtccgccagg tcagcgtcgt aatattatgc
    40141 cgtccgggca acggcatcac gcgaagcggc atcgcgctct tcgcccgtca gctcgctaag
    40201 ttgcccattg cgcatttcca gcaggcggtc ggcgtggata aagtaatgat catcatgact
    40261 gatagcgaaa atagttttac ccatctcctg catcagcggc agcaacacct gataaaactc
    40321 acgacggaag tgtggatcct gatccaccgc ccattcatcc agcaggataa tatcgcgttc
    40381 ttctgccagc gccagcaaca gcgccacgcg ttttttctgc ccttttgata acttcaggtt
    40441 aacaatacgc ccgttgctta actcaagctt atgagccatt ttcagctgcg ccagccactt
    40501 ctcaaccagt tgcgggttag cgggttgccc ctccggcccc agcagttgat caaacagcca
    40561 gacatcggta aacactgccg aaaacagttt gcgataatct tccggttgtt cgccgctgac
    40621 aggtttgcca tccagcaaga tttcgccgct ttgtggctga tacaagcccg tcaacaacat
    40681 cgccagcgtc gattttccgc taccgttacc gccaatcaga aacagcagct cgccacgttt
    40741 gatggtgaga ttaatcggac caacggaaaa cgcgttatcc tgataagcaa acgtcacgtt
    40801 acgcagctcc agcgtttgcc agttgggaaa cgcctgcggg cgcggaaact cagctttgaa
    40861 aggcgcgagc gcgaatttgt tcagcttgtt aaacgccacc tgcgccgtca gcagcgtcgg
    40921 caatgcgcca accgccgaaa gcagcggcgt acgcaggaat aacagcgtca acgaataggt
    40981 cgcagcaacg ttggtatcag cccaaccgag actgttcgcc atccagaaca ccaggccgat
    41041 agcgcccagc atcatgatgt ttgaccagtt cacggcacta agatggaagg tgtctgcgcg
    41101 aataatatga tggcgatact cttgcgcatc ggggatgtag aggttgttaa acacatactc
    41161 ggcgcgttcc cggttcagag tcagctcttt gcgcccttca agtacagttt gaaaatccgt
    41221 gtacagcttg tcttcggttt cacgtagggt cgccatatgt ttgtacaccc gcgccaccag
    41281 cacaaaaccg ccccagatgg tgatcgccat ccagatagcc gttaccagca acattttgcc
    41341 cgacagcatc cacagatacg ccgatgaacc gatagtgaga atgatcccct gccccagttc
    41401 cggcagacgc acaaaagcaa tggtgatatt gcgcacatcg ctggttaatc ccgccagcaa
    41461 cgaggcgcta ccgagttgtt caatgcgctc gacgtgggta tccagaatcc gcttgataaa
    41521 ttcactacgc agtcggtaaa cgaagtgatg ccccaaagtg gtgagcgcca gttgcgatcc
    41581 gagagtgact gccatcaaca gcagcaataa tcccagaaac tccggtaaca ccagcagact
    41641 ggtatccgcc gtttcgataa ggcgctgatt gataaaagca attaagccaa tgcctaatgc
    41701 cgcactggcg aggcttagcg ccatcacact gataaatggc cagcgatact gccgccagac
    41761 aagtacaaga agttccatgc agaaaacccg gacaatgaat tacagcccgc agtttaaaca
    41821 tcttcgcgcg cacagcaata ataattctta tttttattct tttttacctg cctgacggaa
    41881 tgtcaggttg tagcggcagt cggtggtgag tggatgaaac cccgctttca acggttgaat
    41941 accgtgataa aacagccgcg attcaccgcc ccataccacc acatcgccat gttccaacaa
    42001 caaacgtttg agcggatcat tacgtttcag gccaccaaat tgaaaaatcg cgggtaagcc
    42061 cagagaaaca gaaacaattg gcgcgcgcag atccggttcg tctttatcct gatgcagcga
    42121 cagtttcgcg ccaggagcgt agcggttgat aagacaagca tctggctgga aatctggata
    42181 gcccgccgcc gtagccgcac gttgacataa atcatgaaag ctttgtggca tggcgggcca
    42241 cggtttattt gtttgcggat caatgggcga atagagataa ccttgccgat gggtcgtcca
    42301 gcccagatgc ccacagttgg tcatcgccac cgacatggta tatccgcctg gagcgaccat
    42361 ctggcgaaac ggcgactggc tggcaacgtc attaatatcg cggattagtt gctccgcagc
    42421 attaaaagca aaacgccgta aaattaccgc accagccgcc agtggctctt gccacggttc
    42481 agcatcggca aacagatcca acattacctc tcctcatttt cagcttcgcg gcgcagcagt
    42541 tgcgctttac gcgacacgcc ccagcggtaa ccggaaagtg tgccatcacc acggaccacc
    42601 cgatgacagg gtattacgat agccagcttg ttggcggcac aggcgctggc aacggcccgt
    42661 accgctttcg gtttgccgat ggcgttagcc agttgctgat aactgacggt ttcaccacaa
    42721 ggtatcgtgc gcagtgcctg ccagacttgt tgctgaaaag cagtgccgcg aatatccagc
    42781 ggtaacgtca gcggcgtatc gcgttgattg aggctggcga tcacttcacg cacatgttgc
    42841 tgaaacgtca gatcggcagg cgcgttgtcg gcagcgggaa acatctgctg caactcgctg
    42901 attaatgtcg cgtcatcatc gcccagcaat atcgcgcaaa tcccccgctc gctttctgcc
    42961 accaggcaac gacccagctc acaatcagcc agcgcgtaac gcaccgccag attttcgcca
    43021 ccgtgacgga attgtttagc cgtcatgccc agcgtttcat cggctttgcg gtagtaactg
    43081 ctgctgtcgg ggaatccggc gttaagaata gacgtcgtca cgctctcccc tttcgccagc
    43141 gattcgcgca aacggcgagc gcgccaggcc tgttgccagg ctttaggcgt cattccggta
    43201 gtagctttaa acaaccgatg tagatgaaat ggactcatcg ccacctggtc ggctaaggct
    43261 tccagcgtta caggcgtttc ctgttccagc agtcgacacg cgtgggtgat tttatccaac
    43321 cgatgttgcc ggggattggc tttgtctggc tgacaacgtt tgcaggggcg aaatccggcg
    43381 gcgagtgcct cgctggcatt tgcgtagaag gagacatttt cccgcaaggc atgtctggcg
    43441 cggcaagaag gacggcaaaa gatacccgtg gtgcgcacgg cgaaaacgaa ttcgccgtcg
    43501 gcattcgggt cgcgggctaa gacagattgc cagcgttgat cgtcagttaa gcatgtggcg
    43561 tttttcataa tcagctccct ggttaaggat agcctttagt ctgcccggtc accatcacgc
    43621 aaaaaccaac aatcttgcgc tttaattttt ttcgctgaca aggaagcttt taaactgtgg
    43681 tgacatccag gttttaaagc tatcgccttc tttggtgatc atataaaccg ccagcccttc
    43741 ccggcgaaca acctctttgg ctttctccgg cccgagtacc atcaagccag tatcccaggc
    43801 atcggcttcc agcgccgtcg gagcaatcac cgtcacggat accagattgt gttcgatggg
    43861 gcgcccggtt tgcggatcga taacatggga aagacgtttg ccgtccagtt cgtaatagtt
    43921 acggtagctg ccagaggtgc taatcccatg accgttgata tccaccacag cctgaaccgc
    43981 gttttcttta tcggttggtt tttgaatcgc tacccgccac ggctggcctt caccgttcat
    44041 accacggctg ttcagcgcgc cgcccaccga caccagatag cgggaaatcc cttcctgctc
    44101 catcaagcgt gccaggtgat ccgccgcata accttcaccg acggtggaga gatcgacata
    44161 taaatccggc aggtcttttt gcagatattg ctgatgcgac tgattgatga ccgtcaggtg
    44221 ctgtaagccg gttttggctt tcatcgcatc gatctgttcc tggctcggaa tttgaaccgg
    44281 ctgttgttcc gggccaaagc cccacagatt caccagcggc ccgacggtaa tatccatcgc
    44341 accatcggtc cttgccccaa tgcgcagcga ggtggtgacg atatcggcca tcgcttcact
    44401 taccggccac ggcgacaaac tttgcgagtc gttaaagcgc atcagcgcgg aatctttttt
    44461 ataggtcgaa agcagctgat cgtcggcgtc cagctgggtc tgaatctttt ctttaagttc
    44521 ggcactgcgt ttggcatcaa tgcccgggat gctggcacgc cagaaggtac ccatggtttt
    44581 gccttcaaga acggtaactt cagtagcgtg ggttttggcg ggttgtggtt tttgatcgca
    44641 accaacaaag aagagcgcgg caaccagcag tgccacgcgg gtaaagctta tttccatacg
    44701 tgattatcct catgcgaacg gtcgcaagag tacaccaaaa aactgtgttt gtacgctgaa
    44761 aacaatgaaa aaagggcccg caggcccttt gttcgatatc catcgagatt agaactggta
    44821 aaccagaccc agagctacga tgttatcagt gttgatgcca gcgtcacgag tgaactggtt
    44881 gtcgtccagc aggttgattt tgtagtcaac gtaggtggac atgtttttgt tgaagtagta
    44941 ggtcgcacca acatcaacat atttcaggat atcttcgtcg tcgtagttac gaccattgat
    45001 gacacccagg tttttacctt tagactgcag gtaagccagg gacggacgca gaccgaagtc
    45061 gaactggtac tgagcaacag cttcgaagtt ctgtgctttg ttcgcccaac ccaagttgcc
    45121 tacgcgagtt gcgttgtagg tctgggtgta ctgagcagct aggtagatgt tgttcgcgtc
    45181 gtatttcaga ccaccggtgt aggtttcagc gcggtcgcca ttaccgatgt aacgctcatc
    45241 ataacggttc agaccgaagt tctggtcatc agtacgtttg gagctggaaa ctgcagcacc
    45301 gataccgaag ccttcgtaat cataagtaat agaaccgcca acgccgtcac cgttctgacg
    45361 cagagcttca cgaccgttgt tggtcatgcc ttcaccttcc gggctaccgt ttttgccctg
    45421 atactgaaca gcgaagttca gaccgtcaac cagaccaaag aagtcggtgc tacggtaggt
    45481 tgcgaagccg ttaccacgct gctgcatgaa gttgtcagaa ccgtaagtgt caccaccgaa
    45541 ttctggcagt acgtcggtcc aggaagttac gtcgtaaact acgccgtagt tacgaccgta
    45601 gtcgaaagaa cctacatcct ggaatttcag acctgcgaat gccacacggg tccaggagtt
    45661 gttttcgttt tcagcgctgt tgccctggat ctgatattcc cactggccgt aaccggtcag
    45721 ctggtcagta acctgagttt cacctttgaa gccaagacgc atgtaggtct ggtcgccatc
    45781 tacagacttg tcgtcagaga aatagtgcag gccgtctact ttaccgtaca gatctaattt
    45841 gttgccgtct ttgttgtaaa cttcagcagc gtttgctgcg cctgctacca gcagagctgg
    45901 gaccaggagg gacagtactt taactttcat gttattaacc ctctgttata tgcctttatt
    45961 tgctttttta tgccactgca tactgattaa ccctcattaa tcagtcggca agtccattct
    46021 ccccaaaaat gcagaataat ccaacacgaa tatgatacta aaacttttaa gatgtttcat
    46081 ttatcgctat agatatttca aaatgtaaat acaagggaac tttttaagat tattgcggaa
    46141 tggcgaaata agcacctaac atcaagcaat aataattcaa ggttaaaatc aataacttat
    46201 tcttaagtat tcgacagcac tgaatgtcaa aacaaaacct tcactcgcaa ctagaataac
    46261 tcccgctatc atcattaact ttatttatta ccgtcattca tttctgaatg tctgtttacc
    46321 cctatttcaa ccggatgcct cgcattcggt ttttttaccc ttctttacac acttttcatt
    46381 attctgtgct accacagaaa aactataacg cttgttaact atttcacaaa taattaacat
    46441 cagcataatt tccagcaatc tttgtttatt tgcaattaat ttcgttgggc tttttgtagg
    46501 ttatttgtac agcaaaatgg cgcttgtaca tctatttccc cccatacagg atgataaata
    46561 tcacgggaga atagagaatc atcaatcagg taagagtctg gaatttcaca ctgtaccctt
    46621 tatactgccc tatcacttcg cgaagtttta acaggtcata aacacgaatg cgtcagaaag
    46681 agacaacggc cacgacccgc ttttcactcc taccggggag cattacccgc ttctttttac
    46741 tgttgatcat tgtgttactg gtgacgatgg gggtaatggt acaaagtgcc gttaacgctt
    46801 ggctgaaaga taaaagttac cagattgtcg acattaccca cgcaatccaa aagcgcgtcg
    46861 atacctggcg ttacgtgacc aggcagatct acgacaacat tgccgcgacg acctccccct
    46921 cctccggcga aggtttacaa gagacgcgcc tgaaacagga tgtctactat ctggagaaac
    46981 cacgccgcaa aacggaagcg ttaatctttg gctctcacga caactcaacg cttgagatga
    47041 ctcagcggat gtccacttat ctggatacat tgtggggtgc agaaaatgta ccgtggtcga
    47101 tgtattacct gaatggtcag gataacagtc tggtgctgat ctcaacccta ccccttaaag
    47161 atctcacctc cggatttaaa gaatcgaccg tcagtgacat tgttgattca cgtcgtgcag
    47221 aaatgttaca acaggccaac gccctcgatg aacgcgaaag tttttctaac atgcgccgcc
    47281 tagcctggca gaacggtcat tactttacct tgcgtaccac gttcaaccag ccgggacatc
    47341 tggcaacggt cgtggctttt gatctgccga ttaatgattt gatcccaccg ggtatgccgc
    47401 tggacagttt ccgccttgag ccagacgcga cggcaacggg aaacaatgat aatgagaaag
    47461 aagggacgga tagcgtcagt atccacttta acagtacgaa gattgaaatc tcctcggcac
    47521 tcaactctac cgatatgcgc ctggtctggc aggttcctta tggcacctta ttgctggata
    47581 cgttgcaaaa cattctgctg ccactgctgc tgaacatcgg tttgctggcg ctggcgttat
    47641 ttggctatac cacattccgc catttctcca gccgcagtac agaaagcgtc cccagcacgg
    47701 cggtcaataa cgaattgcgc attttacggg caatcaatga agagatagtc tcactgttgc
    47761 cgctcggcct gctggttcac gatcaggaat cgaaccgcac tgtcataagt aacaaaattg
    47821 ccgatcattt gctgccgcat ctgaatctgc aaaacatcac caccatggcg gaacagcatc
    47881 aggggattat tcaggcgacg atcaataacg agctgtatga gatccgcatg ttccgcagcc
    47941 aggtcgcgcc gcgcacacaa attttcatta ttcgcgatca ggatcgcgaa gtgctggtaa
    48001 acaagaaact caagcaggcg cagcgtctgt atgagaaaaa ccagcagggg cggatgacct
    48061 ttatgaaaaa cattggcgat gcgctgaaag aacccgcaca gtccctggcg gagagtgcgg
    48121 ctaaactcaa cgccccggaa agcaaacaac tggcgaatca ggcagatgtg ctggtgcggc
    48181 tggtcgaaga aatacagtta gcgaacatgc ttgcggatga tagctggaaa agtgagacgg
    48241 tgctgttctc cgtgcaggat ttaattgatg aagttgtgcc ttcagtgttg cctgccatca
    48301 agcgtaaagg tctgcaactg ctgattaaca atcatctgaa agcacacgat atgcgccgcg
    48361 gcgatcgcga tgccttacga cgtattttgc tgctactgat gcaatatgcc gtgacctcaa
    48421 cgcaattggg aaaaatcacc cttgaggttg atcaggatga gtcctctgaa gaccgcctga
    48481 cattccgcat tctggacacg ggagaaggcg taagtattca tgaaatggat aatttgcact
    48541 tcccgtttat caaccagacc caaaacgatc gttatggaaa ggcggacccg ctggcattct
    48601 ggctgagcga tcaactggca cgtaaactgg gcggtcattt aaacatcaaa acgcgggatg
    48661 ggcttggtac acgctactct gtgcatatca aaatgctcgc cgctgacccg gaagttgaag
    48721 aggaagaaga gcgtttactg gatgatgtct gcgtaatggt ggatgttact tcggcagaaa
    48781 ttcggaatat tgtcactcgc ctgttagaaa attggggtgc aacctgtatc acacccgatg
    48841 aaagattaat tagtcaagat tatgatatct ttttaacgga taatccgtct aatcttactg
    48901 cctctggctt gcttttaagc gatgatgagt ctggcgtacg agaaattggg cctggtcaat
    48961 tgtgcgtcaa cttcaatatg agcaacgcta tgcaggaagc ggtcttacaa ttaattgaag
    49021 tgcaactggc acaggaagag gtgacagaat cgcctctggg cggagatgaa aatgcgcaac
    49081 tccatgccag cggctattat gcgctctttg tagacacagt accggatgat gttaagaggc
    49141 tgtatactga agcagcaacc agtgactttg ctgcgttagc ccaaacggct catcgtctta
    49201 aaggcgtatt tgccatgcta aatctggtac ccggcaagca gttatgtgaa acgctggaac
    49261 atctgattcg tgagaaggat gttccaggaa tagaaaaata catcagcgac attgacagtt
    49321 atgtcaagag cttgctgtag caaggtagcc tattacatga acaatatgaa cgtaattatt
    49381 gccgatgacc atccgatagt cttgttcggt attcgcaaat cacttgagca aattgagtgg
    49441 gtgaatgttg tcggcgaatt tgaagactct acagcactga tcaacaacct gccgaaactg
    49501 gatgcgcatg tgttgattac cgatctctcc atgcctggcg ataagtacgg cgatggcatt
    49561 accttaatca agtacatcaa gcgccatttc ccaagcctgt cgatcattgt tctgactatg
    49621 aacaacaacc cggcgattct tagtgcggta ttggatctgg atatcgaagg gatcgtgctg
    49681 aaacaaggtg caccgaccga tctgccgaaa gctctcgccg cgctgcagaa agggaagaaa
    49741 tttaccccgg aaagcgtttc tcgcctgttg gaaaaaatca gtgctggtgg ttacggtgac
    49801 aagcgtctct cgccaaaaga gagtgaagtt ctgcgcctgt ttgcggaagg cttcctagtg
    49861 accgagatcg ctaaaaagct gaaccgcagt attaaaacca tcagtagcca gaagaaatct
    49921 gcgatgatga agctgggcgt cgagaacgat atcgccctgc tgaattatct ctcttctgtg
    49981 accttaagtc cggcagataa agactaatca tctgtaggcc agataagacg cgttagtgtc
    50041 ttatctggca tttgcaccga ttgccggatg cggcgtaaac gccttatccg gcctacgatt
    50101 cccattattt caacagatta cattaaagta ggccagataa gacgcgtcag tgtcttatct
    50161 ggcatttgca ctgaatgccg gatgcggcgt aaacgcctta tccgtcctac gaatcccgcg
    50221 atttcctgac cctctcggca tataccgtca gcgtctgttt tatcacatcc agcgttaccg
    50281 gcttcgagag gcagctgtcc ataccggact ccagacaccg ctgcttctct tcagccaacg
    50341 cattagcagt tactccgatt accggcaacg tcagtcccaa ctgacgaatg cgttgcgtca
    50401 agcggtaacc atccatattt ggcatgttga cgtcgctaag cacgatatca atatgattct
    50461 tactaagtac attaagcgca tcgacgccat cattcgcggt tttacattga tagcccaacg
    50521 atcccaactg atctgccagc agacgtcggt taatcggatg atcatccacg accagaatca
    50581 tcatatcgtc gttatcgctg accgctttgt ccgtcgacgg cagagcgtta gcaggatcgt
    50641 cgctctccat ctcgatcaaa taaatacgcg ccaacaatgc cggtagctca tgcggagcag
    50701 ccacactgtg tacccactcc cctggcgctt tctccagcgg aataccaata tggcgacgac
    50761 agaaggtcac taccgctctg ccctgccatt ttttactcac tacctcgtca gtgattaaca
    50821 catcttcggg agtcggttcc tgcccttcgt atgttgtaac gacgatgccg ctgcgctgca
    50881 aactggtttc caggaactga cagagcgacg cattgcggac cgccagccag cagcgtttac
    50941 cactcaaccc ttccacgcct tttttctgcg ggtactgagc gccgtacaac ggaatacgca
    51001 cggtaaactg gctgcccatt cccggttctg aatctaccga gatatcgccg tccatcatgc
    51061 tgatcagttt ttcacaaatc gccagaccca gaccggtccc ctggaagttt cgctgtacgc
    51121 ccgttccaac ctggaagaag ggatcaaaca agcgcaccac ttctttcgcc ggaatcccca
    51181 cgccggtatc gcgaacacgg atagagagat aatcgccatc agcgcgaaca tgcaaaacta
    51241 tacagccggt atcggtgaat tttatggcgt tactcaacag gttggagatg acctgctgta
    51301 aacgcatcgg gtcgccattt aaggccactg gcacatccgg ttcaataaag cagtacaagc
    51361 ctaactgctt gcgtaccacc agcggtaaat agttggcggt gatgtggttc atcacttcac
    51421 gcggtgaaaa ctcacgcggt tcgatcttca actgttccga ttcaatcttc gagaaatcga
    51481 gaatatcgct gataattttc aacaacaggc tggaagagtt gttcattgcc gtcaccagac
    51541 gatcgacgcc tttcggtaac tctttggttt gcaacagatc caggttaccg ataatgccat
    51601 acagcggcgt tcgcagctca tgactgacgg tggcaaggaa catcgatttt gactggctcg
    51661 cctgttccgc tgcttgtgcc atctcctgca acgactcttc catcttcacg cgcgaagaaa
    51721 catccaccag cacacaaatg gccacgtttt cattacgata gcgcgaatgg acgaagctga
    51781 tttgcagatt ggtattgttg ctggtcagga catcaacaaa attgacctgc tgcccacaga
    51841 taatttgcgt cagtcgttgg cggtcctcat gcgtaagcat attgagatag gtatgcgcca
    51901 gttcgttact taaaatattg acgccatcag cggtacgcaa aatgcagata cccactggcg
    51961 cggaggcgac aatcttgcga ttgaactgct catgttcttc cagtcgcagg gcgtcgcttt
    52021 ccgccggaat gaaaatacgt cgctcgtaca tccgcgcgag agtaaacaat gcagctccgg
    52081 caagcacatt cagcaaaatt gcgttaagga tcaacatgcg aatgcgttcc agcaccttat
    52141 caaccggcac cgaatacacg atgcttagcg atgagggtgg cagatttttc ttcagcacca
    52201 gctcccggaa cccttccgta tagccaaacc aggagcgttc ctgcatccag cgaggatcgc
    52261 ccttaatttt actttctggt ccggtaagcg aaatcagggt atgaccattt tcatcaagaa
    52321 tggtaacccc catcggcaac gtacccggta agaaaaagtt ctccatccgg atggtctgct
    52381 cgacacccaa aagcgcctgc aaccggttcg ccagataaac tggcgtcaac gcgtaaaaat
    52441 acccgacgca agggcgcgga ccttcgctga tccagtagag gttactgccg ctatcatctt
    52501 gtggtgcatt tcgatattta ttgatgcgtt catgcaacgc tttcaacgcg gtatcgcgtt
    52561 ccactggcat atcacgcaga ccgaaattgg ccatgcagag gttatcactg ccgattaaaa
    52621 atacccggtt gagatcgtaa gccgcagaaa aattatcgcg ccagtagcgc ataaaccacg
    52681 ccaatgactc cagagaacct cgccaggtgt tactcattgc ggaacaatcg gagtcggcaa
    52741 acagcggttc aaacgcaggc acatccgcct gcgtttctcg tccacgcggg gaaagcacac
    52801 cgttttctgc cgataagcga ttttcggcga tgtacttaag ctctttcatc acatcagagg
    52861 tgcgttgaat aaagcgctga gcctgatcgg aactcagatt aaattcctga cgaatttccg
    52921 attctcgctg atgtaacgca ttaacgatgt aaaaaacgga tgaaaaagca atcaacagcc
    52981 agagcactaa cgccaatgct ctgaacatgt agcgcgaggc tttcagggtt gtacgaaaag
    53041 aagcaaggta tttcaaaggg gagaagctcc gcctcaggtg accgatggag tgtggttaag
    53101 gtagcggtaa aagcgtgtta ccgcaatgtt ctgtcaccat tgcaataatg cgtatcgctt
    53161 agaaaccatt gccttaacca ggaaagccgg agtagatgga cagttctcag tacgaaaaat
    53221 aagaaatgac caatctgccg acgcttgtta atggttatat cctggcgtcg gcagatcgcc
    53281 cagcagtctt atctgaaaaa agagagagcc aggttgtgtg aagggatgga tcaccagtca
    53341 aaacttttca ccagcgtcag ctcgccagca ttacgcatcg gtacaataaa tgtttcctgt
    53401 ttctcattga ccgatccttc atcggtgatc agcgtcagtt gggcggtcgt caattccgtt
    53461 tcgctgcgcc caccatagta gttgatatac acctgatagc gcccgtgaac tggtgctggc
    53521 atggcgaaaa tctcgggtcc gtaccccgtc gtgacatcca tatccagtgc accactgttt
    53581 ttcagcacgg tgttaccgta ccaggcgtgt tcgccatcgg gcgtgacaac atggagatcg
    53641 agatcggtat tgtccgtatc ccacgagaga accagccgta aacgtgcacg aatagttcct
    53701 gtacccggcg ttgagtaaaa ctgcattttt tgccggcttt gcccatccgg gctgatgacc
    53761 tgcacgctgt tgctgccttc agtgaaaata taagggcgtg caaatgaacc atcggattca
    53821 attctctgcg gcatactggc accgttgacc accagccggc cctgctgaac tttacccgcc
    53881 gcagcataat tctttatttt gccgcggatc tgcgctgaga tattttgatc atctgccatg
    53941 ttgacggacg acgccgggta attgatcgat tgtgaaaaac tggcatcttc gccctctgcc
    54001 ggatgccagc cagagagcgg tgcgtcaatc tcgacttcct gcacaccttc actgtgagcg
    54061 acaggcgaaa gtgccaccag taacaacgga agaaaaatct ttcgcatagc attagtccat
    54121 taataattgt cgggtcagcg tttcgatata gttctcatca agcccggtgg gataaccatc
    54181 aaaggccaag tgcagatact catgggttaa atccagccga tcctgaagcg taaagaagtt
    54241 acgaataaac agccgtttct gctgccgatc ggtatagggg aaacctgaga ctaaacgaca
    54301 gaccgcaaac acgtctggtt cgttgtaccc cgtctcgcct tgtaatatac gccgccactg
    54361 cggcattttt ttcgccagcc aggctttcgc tttgggtaat aactggcagg ttgagcgtgg
    54421 agctccccag cggctaaggc tgttgtcggg ataagcaaac gcgagtatct ggtcgtaacg
    54481 ctccccctgc cccgcctggg ccgtggcttg ccgccaggaa agtgtccctt cggtagcgcg
    54541 actgccatga taatgaaccg gatcgcctgc atagatgagc tcctgcgtcc aggcggtcat
    54601 cgttcgcgcc cccgtggtcg ctggcgaagc agaaacacgc tgcgtggcgc tgctgtcggg
    54661 gatggtcagg caatcacctt cccgattggc gttttgctgt aaaaaagtac gtatcgccac
    54721 agtcatggct ttcgctgcct caggtggcgt acttttcgcc tcgcgatcca gaactctggc
    54781 aacgtactct tcacgatcga gatgcgactg caatttcagt ttgccctttt cgctcagtaa
    54841 ggtcgtctca ccgtggctga caaacgtaat gtggttacca ttagtaaacg tcacacgata
    54901 gcgcccatta agtacgctgg gttttactgc cgtcgtactc ttttccgctg tgattttctt
    54961 cagcggatag cgggcaaaca gctccacctc tacacattgc ccactggcaa cctgcgtcgg
    55021 cactggcaaa acccgattaa gcaccgtcgc ataacgggtt agtaccgttt tgctggtgcc
    55081 actgccagta acccacagcg gtgtaccatc ggttaaccag ccagcaaaac caccctgacg
    55141 gatttcctgt ttatcatcgg caaaccagct ccaggttttc acccgtaccc gactaccgag
    55201 catggatgcg acaccgattt tcgcctcgtc cagcaccaca tccagtaaga cttcctgagc
    55261 tttgttttgc gcaggtaacg tcgctaatga atcaagcagc gatttaaccg ttaccgaggt
    55321 ttctggcttc agcgttgtaa gagacgctaa ccacgcaggt gcctgacgct gttgccagta
    55381 ttgcccccac acatctgcgc caatatgcaa ccgctgcggc gcaaaataga gcccacagga
    55441 acgtaccagc gcggtatcgc gcgttatgct ctccccagcc tgacaacaat aaacctcttc
    55501 cggggagttt ccgcgacact ggtagggctg ctccggttga tgggtatcct ccagccaggc
    55561 gtaaacatac agtttccaca aactccctaa cggcgtggtg agcgagtcag gtaatgctga
    55621 gactttggtc acgcctgatg acgacagctg atacagttga tcgtgctgcg caccgcgcag
    55681 caccagttgt aacggcgtct cctccgcaag cgttggcaga gtaaccaacg ccaatagcca
    55741 gacgattctg cgccagttca cttatttcac ctgcatcccg gtccattcac tccctgccgc
    55801 gacactttgc tgcgcaggtg cataggaacg cacataacga gcaggaggca gaacgaattg
    55861 ccctttttgc gagaagcgca gcaaatggcg gaaagtgacc gttcccgtca gttctttcac
    55921 cggcaccata taggccagtt cgcccatttc attacgcgct ttttccagca attgcccctg
    55981 ctgtttcgcg gcgttgggtt tattgactga aatgccccat gttgtgcgct caacgtcggc
    56041 tcccggcggg agcggtactt ccacctgacc atagcgcaga actgcatcct gctcgctggt
    56101 aagcgtaatt tcatcgagat acagcgcatc gctgtcaatc tcattgctgg tcaccggctg
    56161 cagagtaaag ctcatctctt cttcaccggg gataagccga tacaactggc gttcaacggt
    56221 caccggaatg ttactttgtt gagccgtttt tgccggttca cgccagcgga cctgcacatt
    56281 tgcggcgata attcgtcacc aaaagagaga atgtccggca caccctaacc aacccaacgc
    56341 cagtcttcgc cccctccagt taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa
    56401 acaactggag gcattgtcgc catatatttc gccagccagt tcatggccag cgcgcgctca
    56461 atagtggatt gctcagcggt caaaccatta aaatagcggc cgtctgggta gcgtcaccac
    56521 cgctattgag cagcacaacc gtgtgcgcca gcggctgatt gctgtttgcc gctttatccc
    56581 acgcatattg ctgaacctgt ggcatcaccg tggacttcaa ctgcgctttt ttcagcaacg
    56641 tcatggttaa gacgtttgcc accgcatctg ccagtggaga ttccggtgta tcgaggatca
    56701 ggctgtcatt gatatcgcgg atgtcttcct cagagaaatc ttcatcttta gttccgcgcc
    56761 gggcgatagc ttcatccccc cctttcaaca acgttttgca cggcagattc atctcctgtg
    56821 cccacgccag caccagcgcc cgatgcaata acggcatatt atccgcctgc tcggcataac
    56881 tgtcgagcat atgctgccag tattccggtt gttgcgttac gccgagcgcc tggctggcct
    56941 gccagtcggc gtaccatgcc catgccgtaa ggaaggcgtc accattgcca tcttcacccc
    57001 accaggtaaa gcgcgctccg ggccccgcca gttgcatcag ccgcagacgg ttatcctgaa
    57061 tcatctgacg aatgtcgtta gcggcggcac tttgatgatc ggcaagcgaa cgccaggcga
    57121 gactgagcgg gatcagacgg ctaccggtgt tgatcacccc accccacggt tcatcgacta
    57181 acgcatcaag attgttgcgg aaaatctcct gcggcgtttc actactttgt agccggatat
    57241 tactcgcctg ctcgggcaac atcagcgcgt tctcgccacc gccgagcatg acatttttct
    57301 gctgttcaac gggccagctg ttgtccacaa aagacagttt tgtgctaatg ctgtcctgca
    57361 cttgcccatt ttgttgcagt tctgcactta acaagccaga ttgctgaata ttctgcgtca
    57421 gcgaaatata attcgcccct ttgtgcagcg tcagcgtctg gcgcatctca gcgcctgcaa
    57481 atttagtcac cagcgccacc ggttcgttat cctgctgact gaagataaac agtcctgccg
    57541 ccggtttgtc gcccacgcga tacaccgttg gcatactcca cttcatgtag agattttttt
    57601 ccgaacgcag ataagcacgc ccctgcccga ccagcccgtc gccgttcatc ccacgcgcgg
    57661 tgatacgcca gcgggttaac gaatcaggca tcaggaacgt gaaatacgct ttgccttgtt
    57721 tatcggttgt gagtgacggc atccatgccg cggtatccac ctcttcacgc cgtggacgtt
    57781 caagcatttt tactcgccgc tcgctgcggt tagtcgcgcc aggcgcaacc ggctcgctgg
    57841 agagcgcctg gtcatagctg ataaacgaca gactggagct ggtacgcaca ttgttacgcc
    57901 ccagcggata gaaaaatttg ccgatattcg gcgcgatttc tggttgcagc gcgtagatca
    57961 tttcatcgac cacgcctacc gttagctgcg cagaaacagg tttacctttc agcgacgagg
    58021 ttaa
//
LOCUS       DAJTOW010000013        55025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTOW010000013 DAJTOW010000000
VERSION     DAJTOW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.013"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO244_000889"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO244_000889"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5662815.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO244_000890"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO244_000890"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5662816.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO244_000891"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO244_000891"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5662817.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO244_000892"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO244_000892"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5662818.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO244_000893"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO244_000893"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5662819.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO244_000894"
     CDS             7826..9559
                     /locus_tag="OO244_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5662820.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO244_000895"
     CDS             9708..10559
                     /locus_tag="OO244_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5662821.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO244_000896"
     CDS             complement(10546..10887)
                     /locus_tag="OO244_000896"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5662822.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO244_000897"
     CDS             complement(10889..11767)
                     /locus_tag="OO244_000897"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5662823.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO244_000898"
     CDS             complement(11733..14030)
                     /locus_tag="OO244_000898"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5662824.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO244_000899"
     CDS             complement(14081..14401)
                     /locus_tag="OO244_000899"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5662825.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO244_000900"
     CDS             complement(14416..15495)
                     /locus_tag="OO244_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5662826.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO244_000901"
     CDS             15804..16907
                     /locus_tag="OO244_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5662827.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO244_000902"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO244_000902"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5662828.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO244_000903"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO244_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO244_000904"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO244_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO244_000905"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO244_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO244_000906"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO244_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5662829.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO244_000907"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO244_000907"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5662830.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO244_000908"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO244_000908"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5662831.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO244_000909"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO244_000909"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5662832.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO244_000910"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO244_000910"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5662833.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO244_000911"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO244_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5662834.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO244_000912"
     CDS             30120..30728
                     /locus_tag="OO244_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5662835.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO244_000913"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO244_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5662836.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO244_000914"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO244_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5662837.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO244_000915"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO244_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5662838.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO244_000916"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO244_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5662839.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO244_000917"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO244_000917"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5662840.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO244_000918"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO244_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5662841.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO244_000919"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO244_000919"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5662842.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO244_000920"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO244_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5662843.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO244_000921"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO244_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5662844.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO244_000922"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO244_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO244_000923"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO244_000923"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5662845.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO244_000924"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO244_000924"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5662846.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO244_000925"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO244_000925"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5662847.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO244_000926"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO244_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5662848.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO244_000927"
     CDS             complement(44843..45142)
                     /locus_tag="OO244_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5662849.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO244_000928"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO244_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO244_000929"
     CDS             45895..46494
                     /locus_tag="OO244_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5662850.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO244_000930"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO244_000930"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5662851.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO244_000931"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO244_000931"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5662852.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO244_000932"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO244_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5662853.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO244_000933"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO244_000933"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5662854.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO244_000934"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO244_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5662855.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO244_000935"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO244_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5662856.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO244_000936"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO244_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5662857.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO244_000937"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO244_000937"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5662858.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..>55025)
                     /locus_tag="OO244_000938"
     CDS             complement(54612..>55025)
                     /locus_tag="OO244_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCR5662859.1"
                     /translation="LSTDGLTESNVHMGDIFRWGEALIQVSQPRSPCYKLNYHFDISD
                     IAQLMQNTGKVGWLYSVIAPGKVSADAPLELVSRVSDVTVQEAAAIAWHMPFDDDQYH
                     RLLSAAGLSKSWTRTMQKRRLSGKIEDFSRRLWGK"
BASE COUNT        13401 a        14902 c        14082 g        12640 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccagcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaggac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg ggtcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta ccatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagag
//
LOCUS       DAJTOW010000014        53213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTOW010000014 DAJTOW010000000
VERSION     DAJTOW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.014"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(13..324)
                     /locus_tag="OO244_000939"
     CDS             complement(13..324)
                     /locus_tag="OO244_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5662860.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            487..583
                     /gene="ffs"
                     /locus_tag="OO244_000940"
     ncRNA           487..583
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO244_000940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            703..1056
                     /locus_tag="OO244_000941"
     CDS             703..1056
                     /locus_tag="OO244_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5662861.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1098..2648)
                     /gene="pdeB"
                     /locus_tag="OO244_000942"
     CDS             complement(1098..2648)
                     /gene="pdeB"
                     /locus_tag="OO244_000942"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5662862.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2812..3282)
                     /locus_tag="OO244_000943"
     CDS             complement(2812..3282)
                     /locus_tag="OO244_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5662863.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3398..3949)
                     /gene="maa"
                     /locus_tag="OO244_000944"
                     /pseudo
     CDS             complement(3398..3949)
                     /gene="maa"
                     /locus_tag="OO244_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4121..4339)
                     /gene="hha"
                     /locus_tag="OO244_000945"
     CDS             complement(4121..4339)
                     /gene="hha"
                     /locus_tag="OO244_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5662864.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4365..4739)
                     /gene="tomB"
                     /locus_tag="OO244_000946"
     CDS             complement(4365..4739)
                     /gene="tomB"
                     /locus_tag="OO244_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5662865.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5285..8434)
                     /locus_tag="OO244_000947"
     CDS             complement(5285..8434)
                     /locus_tag="OO244_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5662866.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8457..9650)
                     /gene="acrA"
                     /locus_tag="OO244_000948"
     CDS             complement(8457..9650)
                     /gene="acrA"
                     /locus_tag="OO244_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5662867.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9792..10439
                     /gene="acrR"
                     /locus_tag="OO244_000949"
     CDS             9792..10439
                     /gene="acrR"
                     /locus_tag="OO244_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5662868.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10567..13929
                     /gene="mscK"
                     /locus_tag="OO244_000950"
     CDS             10567..13929
                     /gene="mscK"
                     /locus_tag="OO244_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5662869.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14141..14302)
                     /gene="rsmS"
                     /locus_tag="OO244_000951"
     CDS             complement(14141..14302)
                     /gene="rsmS"
                     /locus_tag="OO244_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5662870.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14316..14843)
                     /gene="priC"
                     /locus_tag="OO244_000952"
     CDS             complement(14316..14843)
                     /gene="priC"
                     /locus_tag="OO244_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5662871.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14913..15290
                     /locus_tag="OO244_000953"
     CDS             14913..15290
                     /locus_tag="OO244_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5662872.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15443..15994
                     /gene="apt"
                     /locus_tag="OO244_000954"
     CDS             15443..15994
                     /gene="apt"
                     /locus_tag="OO244_000954"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5662873.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16123..18048
                     /gene="dnaX"
                     /locus_tag="OO244_000955"
     CDS             16123..18048
                     /gene="dnaX"
                     /locus_tag="OO244_000955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5662874.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17381..17445
                     /gene="dnaX"
                     /locus_tag="OO244_000955"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18101..18430
                     /locus_tag="OO244_000956"
     CDS             18101..18430
                     /locus_tag="OO244_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5662875.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18430..19035
                     /gene="recR"
                     /locus_tag="OO244_000957"
     CDS             18430..19035
                     /gene="recR"
                     /locus_tag="OO244_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5662876.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19145..21019
                     /gene="htpG"
                     /locus_tag="OO244_000958"
     CDS             19145..21019
                     /gene="htpG"
                     /locus_tag="OO244_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5662877.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21200..21844
                     /gene="adk"
                     /locus_tag="OO244_000959"
     CDS             21200..21844
                     /gene="adk"
                     /locus_tag="OO244_000959"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5662878.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21976..22938
                     /gene="hemH"
                     /locus_tag="OO244_000960"
     CDS             21976..22938
                     /gene="hemH"
                     /locus_tag="OO244_000960"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5662879.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22935..23894)
                     /gene="aes"
                     /locus_tag="OO244_000961"
     CDS             complement(22935..23894)
                     /gene="aes"
                     /locus_tag="OO244_000961"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5662880.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24046..25350
                     /gene="gsk"
                     /locus_tag="OO244_000962"
     CDS             24046..25350
                     /gene="gsk"
                     /locus_tag="OO244_000962"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5662881.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25483..27159)
                     /locus_tag="OO244_000963"
     CDS             complement(25483..27159)
                     /locus_tag="OO244_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5662882.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="OO244_000964"
     CDS             complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="OO244_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5662883.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28835..30487
                     /gene="ushA"
                     /locus_tag="OO244_000965"
     CDS             28835..30487
                     /gene="ushA"
                     /locus_tag="OO244_000965"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5662884.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="OO244_000966"
     CDS             complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="OO244_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5662885.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31207..32001)
                     /locus_tag="OO244_000967"
     CDS             complement(31207..32001)
                     /locus_tag="OO244_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5662886.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32139..32480
                     /locus_tag="OO244_000968"
     CDS             32139..32480
                     /locus_tag="OO244_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5662887.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32695..35199)
                     /gene="copA"
                     /locus_tag="OO244_000969"
     CDS             complement(32695..35199)
                     /gene="copA"
                     /locus_tag="OO244_000969"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5662888.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35461..36393
                     /gene="glsA"
                     /locus_tag="OO244_000970"
     CDS             35461..36393
                     /gene="glsA"
                     /locus_tag="OO244_000970"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5662889.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36396..37688
                     /locus_tag="OO244_000971"
     CDS             36396..37688
                     /locus_tag="OO244_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5662890.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37813..38220
                     /gene="cueR"
                     /locus_tag="OO244_000972"
     CDS             37813..38220
                     /gene="cueR"
                     /locus_tag="OO244_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5662891.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38221..38679)
                     /locus_tag="OO244_000973"
     CDS             complement(38221..38679)
                     /locus_tag="OO244_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5662892.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38676..39593)
                     /locus_tag="OO244_000974"
     CDS             complement(38676..39593)
                     /locus_tag="OO244_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5662893.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39739..40416
                     /gene="fetA"
                     /locus_tag="OO244_000975"
     CDS             39739..40416
                     /gene="fetA"
                     /locus_tag="OO244_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5662894.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40403..41182
                     /gene="fetB"
                     /locus_tag="OO244_000976"
     CDS             40403..41182
                     /gene="fetB"
                     /locus_tag="OO244_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5662895.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="OO244_000977"
     CDS             complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="OO244_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5662896.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="OO244_000978"
     CDS             complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="OO244_000978"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5662897.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="OO244_000979"
                     /pseudo
     CDS             complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="OO244_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43552..44238
                     /locus_tag="OO244_000980"
     CDS             43552..44238
                     /locus_tag="OO244_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5662898.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44235..46650
                     /locus_tag="OO244_000981"
                     /pseudo
     CDS             44235..46650
                     /locus_tag="OO244_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47363..48457)
                     /gene="mnmH"
                     /locus_tag="OO244_000982"
     CDS             complement(47363..48457)
                     /gene="mnmH"
                     /locus_tag="OO244_000982"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5662899.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48526..49452)
                     /gene="allS"
                     /locus_tag="OO244_000983"
     CDS             complement(48526..49452)
                     /gene="allS"
                     /locus_tag="OO244_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5662900.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49682..50164
                     /gene="allA"
                     /locus_tag="OO244_000984"
     CDS             49682..50164
                     /gene="allA"
                     /locus_tag="OO244_000984"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5662901.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STQRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50242..51057
                     /gene="allR"
                     /locus_tag="OO244_000985"
     CDS             50242..51057
                     /gene="allR"
                     /locus_tag="OO244_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5662902.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51147..52928
                     /gene="gcl"
                     /locus_tag="OO244_000986"
     CDS             51147..52928
                     /gene="gcl"
                     /locus_tag="OO244_000986"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5662903.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKHNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52941..>53213
                     /locus_tag="OO244_000987"
     CDS             52941..>53213
                     /locus_tag="OO244_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5662904.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12832 a        13692 c        14016 g        12673 t
ORIGIN      
        1 gcccggagag ggttagtagt tccagcgata acgctgcaag tcgatttgcc cgcttcccga
       61 taccatcaca ccttctgcca gtaatgcctg tcgctgacgc tgtaaatccg gcccggttag
      121 cgaaattgtg ccatggcgat taaccacccg gtgccagggt aaggtgctgc cttcagggag
      181 acgctttaac acaccgccca cctggcgcgc ggcgcggggc gatcccgcca gtttcgccac
      241 atcaccgtaa gtggtgacat agccttcggg aatagcggcg acgatttgcc agacgcgttg
      301 gggaaatgaa tcttcttttt ccatcttttc ttcctgaggt aatttttcag cataatctgg
      361 aaaaacgccc gagtgaagtc gcattgcgca agaaaccagc atctggcacg caatgggttg
      421 caattagccg gggcagcagt gataatgcgc ctgcgcgttg gttttcaacg ctctcaatgg
      481 gggctctgtt ggttctcccg caatgctact ctgtttacca ggtcaggtcc ggaaggaagc
      541 agccaaggca gatgacgcgt gtgccgggat gtagctggca gggcccccac ccatttctgc
      601 ctcccaccgt ttcgtcaaaa aatcccagca tggctaaact ttaaccacaa ctgacgtcgc
      661 aagaattgtc tggctgcgca gtacgcttcg gaggtatgtc tgatgaagta tgttgatggt
      721 tttgtggttg ccgttcctgc cgataaaaag gatgcctatc gggaaatggc cgctaaggcc
      781 gcgccattgt ttaaagagtt tggcgcgctt cgtattgtcg aatgctgggc cagcgatgtg
      841 ccggatggca aagtgaccga ttttcgtatg gcggtgaaag cggaagagaa tgaagaggtt
      901 gtctttagct ggattgaata cccttcaaaa gaggtccgcg acgcagctaa tcaaaagatg
      961 atgtcggacc cacggatgaa agagttcggc gagtccatgc cgtttgacgg caagcgaatg
     1021 atctatggcg gattcgagtc aatcatcgac gaataggtag cgtgacgggc tgtgcggcgt
     1081 gccgtcagcc caaaaattca caaatgttgc tcggcccagc gtaagaaatc ttctttcggt
     1141 aatgccttgc tgtagagcca gccctgaccg tagtgcacgc catgctggcg tagccactct
     1201 tcttgtttac tggtttcgat tccctccgct accattttca gtttcagtgt ttttgccatt
     1261 tcgatgatat gcggcgtgac atttttatat tccagcgcat caacgaaaga tttatcgatc
     1321 ttcagaatgt cgacatccag atcctgtaaa taacttaaac ttgaataccc cgtaccaaaa
     1381 tcatcaagat aaatttcatg gcccgcctcc cggtagcgag aaattatcgg ggcgctggtt
     1441 ttcggatcgg caaactcgcg ttcagtgagt tcaagcgcga tctgtctggg attaacctga
     1501 tagtgattga tcatttcacg cagcaattgc gggatttttt ccgaggtgag cacggtggat
     1561 tcaagattga tcgaaatatg ctgctgtgga tgctgacgca agcagtcgcc catatcttca
     1621 aagacgcttc ttataatcag tagcgtcaat ggctcagaaa ggcccgtttg ctgtgccagc
     1681 ggaataaaac tatctggtga caaccaacta ccgtctgtct gcggccagcg cgccagtgcc
     1741 tcagcaccga caattttgcc attggctaag gagacaatcg gctgatagtg cacgcaaata
     1801 tcacgatttt cgatagcatc ctgcagccga tgatgcggtg attggatacg gcgcaaaata
     1861 cgcagcacaa acatcgctgc cagcaggcca atcaccaacc cgagcggtaa ccaaataaag
     1921 acttgccgat gccagccttt ctgcaacatt ttcgttgaag cccaggtgat gatcgaaata
     1981 ttcatctcca ggaagggcag tatatcgtaa atgatcccat tattttcgat atgctcaccg
     2041 ggtgtttttt gtagcctggt aataattccc tgagcaattt catcgctgct ggttatgaca
     2101 acgttatggg cattgccaat aatggcggca tcaatttgcc atgaactata gggaatgaca
     2161 tcaataaagg aagcggggtc gatcatgacg acataatgtg ccgttcccat ggcgaccatg
     2221 taacggataa tgcctaaatc gttatgcgat gttaaccaga cacgataacc atctttcgaa
     2281 attttaccta gctcgggaaa ggtatcgggc ggactttcat gctccagaga cgaacactgg
     2341 ggaacgttgt tatcgatata aaccacttcc tgaatatagc gataactgta agatacccga
     2401 cgcatttcca tgagatgggc ttcgctacag gcagcgcctt gccatctttc cagctcctgc
     2461 agcgcatctt tcccttgtgt cgccacctta ttggctcgaa tagcgacgcg ggaggaatag
     2521 gtatccagct cttcaataaa cgatgtttct acctgctgat gggccagcca gatgcttaag
     2581 ccgacaggca gcaatactga aagaatcagt actcccgaaa taaggccgac cagatgtcgt
     2641 gttctcacca gcatatcctt attaacacag tgtagggaaa gtatatctga ttgtggaaaa
     2701 aggctggcag gagataagga ggtagagctg gtatttcatg aagtgatggg tggaaacgat
     2761 cattgtcatt tgaccaggcg cagagcttta cgcctggtat gtaaccctaa cttaagatgt
     2821 tgattcggcg cgagcgaggg taaaaatatc gtaaaaagac agttcacctt tacggacgat
     2881 cattttttgc agttgttcct tatgaacatc cgcgacacgc ggagagttca agatggcttc
     2941 aaccagcgcg gcaggtacaa agcgcgtgtt gtaccattcg ccgttatagc aaacgcggaa
     3001 atccagcacg tcgatatctt catagcggta gcgtgggtag acatcgaaaa agaagaaacc
     3061 attaagcagg ataaataata caaccagtag ccagacagag cctgacagcg tttcggactg
     3121 caacatcacc gccagggtgg caaaaaaagc aacgtacata ccgataaaca gccccggatg
     3181 tttacggata aaactgatac taaagcgggg tttgttgtcg cgtttttcgc gggtattcag
     3241 agactcaatc gtttcggtca gcaggcgttg tatttcggtc atcgtttgcc ttcgtggtat
     3301 ctgcaacttt ccagggaaca ccctatttta acgtgggaat agtagagggg aagtaacaga
     3361 tctaccacaa ttttgcataa cagttgcgaa aaaccgatta caatttttta attattctgg
     3421 ctggattacc gcccacgaca acgttgtccg ggacaccttt tgtgacaact gcacctgaag
     3481 ctaccacgac gttatcacca atggtcacac cagggttaat gaccgcgcgt ccgccaatcc
     3541 agacgttatt accgatggtg acgggtttcc ccagttcagc accgctatta cgtgctacag
     3601 ggtcgatggg atgtgttgcc gtgtagatat gaacgcctgg tgccaacata cagttatcac
     3661 cgatgcgaat agggcagaca tcaagcatca cgcaatcgaa gttggcgaaa aaattattgc
     3721 cgagaaaaat gttatagcca taatcacagc gaaacgttgg ctcaatataa gcctctgtca
     3781 cctgaccgaa taaattagcg agaatttact ggcgtaatgt gtgctcttcc gccagggaat
     3841 gattgtatcg gtgaataagc tgacgagcgc gcaggcgatc gcgagataac gtctcatctg
     3901 ccgagcgata caactcacca gcaatcatct tttctttttc tgtgctcatt actgacctcc
     3961 ggttgctgaa aatgcggcaa cacagtaaga cgaaaagtca gtgaagagaa cgttctcatt
     4021 acatctgtgt gataaagatc acatagggta tgtcttcatg gcgaaaaagt ataaaattct
     4081 taataaacag ccggttatag caccgaaagc gaattattta ttagcgaata aatttccata
     4141 ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa ttcggcgagg cggtgatctg
     4201 cggctgagta aaataccgcc agttcattat ctgataattc gtatttattt ttctcgataa
     4261 cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa tcggtttttg
     4321 ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca agataaactc
     4381 gcaggattct ctttcgtcgc gttgacaaaa caacggaata gtcgattacc tgacttccgc
     4441 catttctgaa gagcctgcat attaatacca taactgctga acaacataaa ggtgtcatcc
     4501 agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat tttgtaatta
     4561 agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact ggaggttgat cgccgaggtt
     4621 gggtcgttaa cccaaccatg attgctttct tcaaggtttg caagacagtc atgatacagg
     4681 gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta ttcatccata
     4741 acgcgtcccc ttcttagtgg ttgaactaac ggacaccttt cgggatggaa aaaacttact
     4801 gacctagact tgccctctcg tttgttagct taactataag ccactctttg caggttttca
     4861 tcgcattatt acgaaacatt acaattagga aggaatcggg acaggactca ctttttcata
     4921 ctagcactaa cccgcagcag gtaaaagcag tttgctcgaa ctgttcataa catttcagga
     4981 ttatcgccag atgaaattgg tgacactaat accaggattg ctctgaatat gacgtaataa
     5041 ccgaggaatg aataaagaat taccgcaaat aattaagaat agcctctaaa tgattatgga
     5101 ttattaaatc tattagcgct acttaatata aattaactaa caatcagtat tttttatgaa
     5161 ttttatacgt ggttaatact ggttttcgta tgagatcctg agttggtggt tcaattactc
     5221 cttaatgttc gtaggttatg cataaaaaag gccgcttacg cggccttagt gattacatgt
     5281 tgtatcaatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg cggctaaagc
     5341 ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc gttgcggtca
     5401 ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca gcaccagtac
     5461 tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg atcggacgta
     5521 aacgcatccg caccgcatca agcgtcgctt caatcagacc tttaccttct ttatccatca
     5581 agtctttggc gaattcgacg ataagtatcg cgttcttcgc cgacaaccca atggttgtga
     5641 gcagtcctac ttggaagtaa acgtcattgg tcaggccacg gaaggtggca gccagcaacg
     5701 caccgataac ccccagcgga acgaccagca taacggagaa cggagtcgac cagctctcgt
     5761 acagcgccgc cagacacagg aatacgacaa tcaacgaaat cgcgtacagt gaaggtgcct
     5821 ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagcca acaccggtag
     5881 gcagtttgct cgccagttgt tccatcagct ccattgcttc accggtactt ttacccggtg
     5941 ccgcctggcc taagatttcc atggatggca ggccgttgta acgttccaga cgcggcgaac
     6001 cgtactccca acgagaagag gagaacgccg agaatggcac catctgacca tcagcagcac
     6061 gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct gacatgacgt
     6121 aaactttctt cacacgaccg cggtcgataa agtcgttcac atagctaccg ccccatgcag
     6181 cgcccagagt ggtgttaatg tcgttgatag aaacacccag cgcctgcgct ttttcctggt
     6241 cgatatcaat cttaaactgc ggggtatctt ccagaccgtt tggacgtacg ctggtcagca
     6301 tatcagggtg cttcgctgct tctgcaagca gctggttacg cgcctgagtc agtttttcgt
     6361 gaccaaggcc agcctggtca atcagctcaa agtcaaagcc ggttgcagta cccagttcca
     6421 cgattgcggg caggttaaag gcgaaaacca tcgcatcttt gatttgcgag aaagcgcgtg
     6481 ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg gcccagtcct
     6541 tcaaggaaac gaacgcaata ccggtattct gaccacgtcc cgcaaagccg aagccgttaa
     6601 cggcgaacac cgactcaacg ttgttctttt ctttggtcag atagtaatgc gttacctcat
     6661 tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg cagctgaacc atggtcataa
     6721 atacgccctg gtcctcatct ggcaagaagg agcttggcag acgcacgaac agataggcca
     6781 tgccgaccac gatgatcaga tacagcacta ggtaacgccc cgtactgcgc agaataccgc
     6841 ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat gcggttcaac cagccgaaga
     6901 agcctttttt accttccccg tgatcgcctt tggcaatcgg tttcagcatg gtggcacaaa
     6961 gagccggagt caggatcaac gctaccagta ccgacagcgc cattgctgaa acaatggtaa
     7021 tagagaactg acgatagata gcaccagtag aaccgccaaa gaaggccatc ggtacgaata
     7081 ccgccgacag taccatcgcg ataccgacca gagcgccctg aatctgcccc atcgacttac
     7141 gggtagcttc ttttggcggc aaaccttctt ccgccataac acgctccacg ttttctacca
     7201 caacgatagc gtcatccacc aacaagccga tggcgagcac catcccgaac attgttagcg
     7261 tgtttatcga gaagccaaag gcggcaagga cggcaaaggt cccgagcaat accaccggta
     7321 cggcaatggt cggaatcaac gtcgcgcgga agttctgcag gaacagatac ataaccagga
     7381 acacgaggat gatcgcttcg accagcgttt taaccacttc gtgaatagag attttcacga
     7441 acggcgtggt gtcgtacggg taaacaattt tcagacccga cgggaagaac ggttccatct
     7501 tcgccagttc agcacggatt gccgcagcgg tatccagcgc gtttgcaccg gtcgccagct
     7561 tgatccccag accggaagca ggttggccgt taaactctgc gatgatgtcg tagttctcac
     7621 cacccagctc aatcttcgcc acatcacgca gcagtacgcg ggaaccatcc tgattcactt
     7681 tcagcaggat tttgccgaac tcttcagtag aggtcagacg cgtctgagca ataatagagg
     7741 cgttaagctg ttggcctttc accggcggcg taccaccgag ctgacccgcc gcaacctggg
     7801 cgttctgcgc tttgatggcg gtaatgacat caaccggcgt tagctggaat ttgttcagct
     7861 cattcgggtt catccagata cgcatcgcgt actgtgaacc gaacaactga acatcaccca
     7921 cgcccgacgt acggctgatg gcatctttca tattcgccgc tacgtagtca gagatatcct
     7981 cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac catcaggaag ctgctggatg
     8041 atttctcaac gctcacccct tgctgctgaa cttcttgcgg cagcaacggc atcgccagct
     8101 gcagtttgtt ctgcacctga acctgcgcga tatccgcatc agtaccagac tcaaaggtca
     8161 gggtgatctg cacggtaccc gtggagtcac tgttagagga catgtacatc aggttatcga
     8221 taccgttcat attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt ttcgcatcag
     8281 cgccggggta ggaggcggag atcgttactg ccggcggtgc aatcgtagga tattgcgcca
     8341 ccggcagttt gaggatcgcc agcccccctg ccaacatgat gataatggcg atcacccacg
     8401 caaaaatcgg gcgatcgata aagaaattag gcatgtctta acggctcctg tttaagttaa
     8461 gacttggact gttcaggctg agcaccgctt gcggcttgct ggttattatc agcggtaact
     8521 tcttgtgctt ttacctggac accaggacgc actttctgca gcccacttat tactacgcga
     8581 tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc caatagcctg gcttgcaacg
     8641 atcggacggg tttccacttt gtcatccgcg ccaactacca gtacggtggc atcaccacgc
     8701 ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt ttggattaag cccttcttcc
     8761 agacgtgcgc gcacgaacat acccggcagc agagtgtgat ccgggttcgg gaagatagcg
     8821 cgtagggtga tagacccagt ggtctgatca acggtaacgt cagagaattc cagcgtaccg
     8881 tcctgcggga acttaatgcc gtcactggtg atcagcgaca ctttggcttt gccgttctct
     8941 tgtttcagcg tgccattcgc cagttcctgt ttcaggcgaa ggaagtcgtt gctggactgg
     9001 gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg ccagcacagt cgcctgaccg
     9061 ttctgtacca atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct aatcggagag
     9121 gtgactttgg tgtaagccag attgatccgc gcagtttcaa cggcagcttt cgccgcagtt
     9181 accgcagcat tcgcctgttg cgcatcagcc agagcctgat cgtacccttg cttactgatg
     9241 tactgagtac cgagcagttt ctgataacga ttcaccgtca attgcgcgat attggctgca
     9301 gcctgggctt tcgccagatc acctttcgca ctgtcgtatg tcgcctgata ggtcgcagga
     9361 tcaatctgat agagagagac accagcttcg atgtcgctac cttctttgaa attacgcttc
     9421 aggataatcc cgctaacttg aggacgaact tctgcgatcc ggtaggcact ggtgcgacct
     9481 ggaagctcgg ttgtgatctg cagaggttca gttttgactg ttactacgcc aacggcgggc
     9541 atctgctggc caccttgttg ggcctgtttg tcgtcacatc ctgttagggc taagctgcct
     9601 gagagcatca gaacgaccgc cagaggcgta aaccctctgt ttttgttcat atgtaaacct
     9661 cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa taaacccatt gctgcgttta
     9721 tattatcgtc gtgctatggt acatacattc acaaatgtat gtaaatctaa cgcctgtaaa
     9781 ttcacgaaca tatggcacga aaaaccaaac aagaagcgca agaaacgcgc caacacatcc
     9841 tcgatgtggc tctacgtctt ttctcacagc agggggtatc atccacctcg ctgggcgaga
     9901 ttgcaaaagc agctggcgtt acgcgcggtg caatctactg gcattttaaa gacaagtcgg
     9961 atttgttcag tgagatctgg gaactgtcag aatccaatat tggtgaacta gagcttgagt
    10021 atcaggcaaa attccctggc gatccactct cagtattaag agagatatta attcatgttc
    10081 ttgaatccac ggtgacagaa gaacgacgtc gattattgat ggagattata ttccacaaat
    10141 gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca acgtaatctc tgtctggaaa
    10201 gttatgaccg tatagaacaa acgttaaaac attgtattga agcgaaaatg ttgcctgcgg
    10261 atttaatgac gcgtcgggca gcaattatta tgcgcggcta tatttccggc ctgatggaaa
    10321 actggctctt tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat tacgtcgcca
    10381 tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact aacgaataac
    10441 cctgaatctg actccaggat ttttcctgga cattttcgtc gttgctattc tggttcactg
    10501 cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc ttcaggttca gaaaccttca
    10561 ttcatcatga ctatgttcca gtattacaaa cgatcacggc attttgtttt ttcagcattt
    10621 attgcttttg tttttgtctt gttatgccag aacacggcgt ttgcgcgggc gtcatcgaat
    10681 ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg actcactaaa taaacaaaaa
    10741 gatctttctg ctcaggacaa actggtgcag caggatctga cagatacatt agccaccctc
    10801 gataaaatcg atcgcataaa agaagagaca gttcagctac ggcaaaaagt cgctgaagcg
    10861 ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt cgataacgac
    10921 gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc agctggaaac tcgcgttgcc
    10981 caggcgctgg acgatttgca aaacgcacaa aacgatctgg cgtcttataa cagccagctg
    11041 gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc ttcgcagcag
    11101 ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg gcgagactgc cttacgtccc
    11161 agccagaaag tgttaatgca ggctcagcag gcgttgctga atgcggagat tgaccagcag
    11221 cgtaaaagcc tggaagggaa caccgtcttg caggatacct tgcaaaagca acgtgattac
    11281 gtgacggcga acagcgcccg tctggagcac cagttgcagc tgttgcaaga agcggtaaac
    11341 agcaagcgcc tgactttaac tgaaaaaacg gcgcaggaag ccgtctcccc ggatgaagcc
    11401 gcgcgtattc aggctaatcc gctggtgaaa caggaactgg aaattaacca gcagttaagt
    11461 cagcgtctga ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa cattaaagtc
    11521 aaaaactggc tggagcgggc gctgcaatcg gaacgcaata ttaaagagca gattgccgtc
    11581 ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac gctgccctcg
    11641 gcggatgaac tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga acagtttgaa
    11701 gttaaccagc agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa actggaagaa
    11761 ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt ggttgatatg
    11821 cgtcgcgaat tgctggatca actcaacaaa cagttgggta accagctgat gatggccatt
    11881 aacctgcaaa tcaaccagca gcagttaatg agcgtatcga aaaacctgaa atccatcctg
    11941 actcagcaaa tcttttgggt gaacagtaac cgtccaatgg actgggactg gatcaaagcg
    12001 ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa tcacggtgaa ctgggaaaaa
    12061 gcctggcctg ccgtttttat cgctttcctc gccggtttgc cgctgctgtt gattgccggg
    12121 ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac agaaactggc ttccgctgtg
    12181 ggttccctgc gtaacgatag ccagctcaac acaccaaaag cgatccttat cgacctgatc
    12241 cgcgcgctgc cggtgtgcct gattattctc gcggttggcc tgattctgtt gaccatgcag
    12301 ctcaacatca gcgaactgct atggtcgttc agtaaaaaac tggcgatttt ctggctggtg
    12361 tttggcctgt gctggaaggt actggagaaa aatggcgttg ccgtgcgtca cttcggcatg
    12421 ccggagcagc agaccagcca ctggcgtcgg caaattgtcc gcatcagtct cgcgttgctg
    12481 cctatccatt tctggtctgt ggtggcagaa ctttccccgc tgcatctgat ggatgatgtg
    12541 ctggggcagg cgatgatttt cttcaacctg ctgctgattg ccttcctggt atggccaatg
    12601 tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc gactagtcac cattaccgtg
    12661 ctgtcgatta tcccgattgc attgatggtg ctgactgcca cgggttattt ctacaccacg
    12721 ctgcgcctct ccgggcgttg gattgaaacc gtttatctgg tgatcatctg gaacttgctc
    12781 taccagacgg tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgcg
    12841 ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc gccggaagaa
    12901 cccaccattg cactggagca agttaaccag cagacgctgc gtattaccat gttgctgatg
    12961 tttgcgctgt tcggtgtcat gttctgggca atttggtccg atttgatcac cgtgttcagc
    13021 tatctcgaca gcatcacgct ctggcattac aacggcactg aagctggcgc tgcggtggtg
    13081 aaaaacgtca ccatgggcag tctgttgttt gcgattatcg cctcaatggt ggcctgggcg
    13141 ttgattcgca acctgcctgg tttactggaa gtgctggtgc tctcgcgact gaatatgcgc
    13201 cagggcgcgt cgtatgccat tactaccatc cttaactaca tcattattgc cgttggtgcg
    13261 atgacggtgt tcggatcgct gggcgtctct tgggataaac tccagtggct ggccgcagca
    13321 ttatccgtag gtcttggttt tggtttacaa gaaattttcg gtaacttcgt ctccggtttg
    13381 atcattctgt tcgaacgtcc ggtgcgtatt ggcgatacgg taaccattgg tagcttctcg
    13441 gggacggtaa gtaagatccg tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa
    13501 gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga tcaactggtc gttgactgac
    13561 actactacgc gtctggtgat ccgtctcggc gtggcctatg gctccgatct ggaaaaagtg
    13621 cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg tgatgcacga accaatgccg
    13681 gaagtcttct ttacggcatt tggtgccagc acgttggatc atgagctgcg tctgtatgtg
    13741 cgtgaactgc gtgaccgtag ccgtactgtc gatgagctga accgtactat cgatcagctg
    13801 tgccgtgaaa acgacatcaa cattgccttt aaccagcttg aagtgcatct gcacaacgag
    13861 aagggcgatg aggtgacgga agtaaaacgc gactacaaag gcgacgatcc gacaccagcg
    13921 gttgggtaat aaccaaaggg ggcgacattt agttgccccg agattactga caaagtgcgc
    13981 gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg caagaattca
    14041 atatattgca ggagcggtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
    14101 cccgcaatct taggggacat ttagcggccc catttatttc tcacttttcc gcctcatcat
    14161 cgtgcgttaa tttcttttca taatcacgct ttacaatatc cagcgcgcgc agcacggtac
    14221 tggcagggat ctgattttcc tccagcagca caatcaaatc gacagccagt ttgacatcgt
    14281 caggggcatt ttcaagtgac atactctttc cattgctagc gggttaagcg cgctagcctg
    14341 ttttcgattt tttccagcgc atggcggcag cgtgccaggc gcgcttcata ggcttccact
    14401 tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc gcgccaggcg ggctctgcgt
    14461 tcggcaacca tctcacgtag ccgccgctca aaatcctgat gctgaatacg tttacgctgc
    14521 cagcgggaaa ttttcggtgg cgcactgtcc cactcgcgca atgaccaggc ggaggcttca
    14581 cgcgcgatgg cttccagttg tgccgccaga tgttccgcca gccaggccac ttgcggcagc
    14641 tgttgctgct caactgcatg acgaagcgca gccagattat cgcccgcctc gtcgagacaa
    14701 gactgtagtg ttgtcgcacg agtctgaaaa agatgcctgt cgaaacgagc acttagcgtg
    14761 gcgaactgtg acaccggggc acaacgctga cgcagcgtag cgagctgtcc ttccagtttt
    14821 tccagcagca gggcggtttt cacgataaga cctcaatgaa aatgataatt gttatgctaa
    14881 agtagccact atgtaagctg acaacactca atatgcaacg aatcatttta atcatcattg
    14941 gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt accggtatta ccgacgacgc
    15001 cgtttatcct gctggcggcc tggtgctttg cccgttcttc cccgcgcttt cacgcctggt
    15061 tgctgtaccg ctcatggttt ggcagctatc tacgtttctg gcagaaacat catgcgatgc
    15121 cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct cacgtttgcc atttctctgt
    15181 ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt
    15241 tctatatgtg gcgaattccg gtgattgatg aaaagcaaga aaagcactga agcacaacaa
    15301 tcgcagttgc aattattgcg tacagccagt acattctggc gttttcgagc acaggcgcag
    15361 gcggtcaaag gttaaacaac agttactttt gatacgttta aaacgcgccg tgagtaccac
    15421 cgtaacaagc aggcatacac ttatgaccgc gactgcacag cagcttgagt atctcaaaaa
    15481 tagcatcaaa agcattcagg actacccaaa acccggcatt cttttccgcg atgtcaccag
    15541 cttactggaa gacccgaaag cttacgctct cagcatcgac ttgctggttg agcgttacaa
    15601 aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc
    15661 ggtagctctg ggtctgggcg ttggctttgt accggtccgt aaaccgggca aactgccgcg
    15721 tgaaaccatc agtgaaaatt acgacctgga atacggcacc gatcagctgg aaatccacgt
    15781 tgatgccatc aaaccgggcg acaaagttct ggtggtggac gatctgctgg caaccggcgg
    15841 cactatcgaa gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg ctgacgctgc
    15901 gttcattatc aacctgtttg atctcggcgg cgaacagcgt ctcgaaaaac agggcattac
    15961 cagctacagc cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt gctgcccacg
    16021 ctacggacag cacaagatgt gcattcagcc tcgccgttct gacggggctg tgttagcatt
    16081 accccttcgt gaatccacct tccagcgttt cagagcctgc caatgagtta tcaggtttta
    16141 gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc
    16201 gcactggcga acggcttgtc gttagggcgt attcatcatg cttatctttt ttccggcacc
    16261 cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc
    16321 ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc
    16381 tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacca aagttgaaga tacccgcgac
    16441 ctgctggata acgtccagta cgctccggcg cgtggtcgtt tcaaagttta tctgatcgac
    16501 gaagtgcata tgctgtcgcg ccacagcttt aacgcactgt taaaaaccct tgaagagccg
    16561 ccggagcacg ttaagtttct gctggcgacg accgatccac agaaattgcc ggtgacgatt
    16621 ttgtcacgct gtctgcaatt tcatctcaag gcgctggatg tcgagcaaat tcgccatcag
    16681 cttgagcaca tcctcaatga agaacatatc gctcacgagc cgcgggcgct gcaattgttg
    16741 gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc tgaccgacca ggcgattgcc
    16801 agcggtgacg gccaggtttc aacccaggcg gtcagtgcga tgctgggtac gcttgacgac
    16861 gatcaggcgc tgtcgctggt tgaagcgatg gtcgagggca acggcgagcg cgtaatggca
    16921 ctgattaatg aagccgctgc ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc
    16981 ggcttgttgc atcgtattgc gatggtacaa ctttcgcctg ctgcacttgg caacgacatg
    17041 gccgccatcg agctgcggat gcgtgaactg gcgcgcacca taccgccgac ggatattcag
    17101 ctttactatc agacgctgtt gattggtcgc aaagaattac cgtatgcgcc ggaccgccgc
    17161 atgggcgttg agatgacgct gctgcgcgcg ctggcgttcc atccgcgtat gccgctgcct
    17221 gagccagaag tgccacgcca gtcctttgca cccgtcgcgc caacggcagt aatgacgcca
    17281 accctggtgc cgccgcaatc agcgccgcag caggcaccga ctgtaccgct cccggaaacc
    17341 accagccagg tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca
    17401 aaaaagagtg aaccggcagc cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa
    17461 agactggctt cggtcaccga tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa
    17521 gcgccagcca aaaaagaagc gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa
    17581 gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa
    17641 ctggcggtga agctagcggc agaagccatt gagcgcgacc cgtgggcggc acaggtgagc
    17701 caactttcgc taccaaaact ggtcgaacag gtggcgttaa atgcctggaa agaggagagc
    17761 gacaacgcag tatgtctgca tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca
    17821 cagcaaaaac tggctgaagc gttgagcatg ttaaaaggtt caacggttga actgactatc
    17881 gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt ggcgtcaggc gatatacgaa
    17941 gaaaaacttg cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt
    18001 cggtttttcg atgcggagct ggatgaagaa agtatccgcc ccatttgatc gtaagcacag
    18061 cttacgttcg tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct
    18121 gggtaacctg atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga
    18181 gatcgcgcag ctggaagtta ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa
    18241 cggtgcacac aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga
    18301 gatgctggaa gacctggtgg ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac
    18361 gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg gctttaagat
    18421 gccgttctga tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgtctg
    18481 ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt
    18541 agcggcggga tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc
    18601 gccgattgcc gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt
    18661 caggaaaacg gtcaaatctg tgtggtggag agtccggcgg acatctacgc cattgagcag
    18721 acggggcagt tttctggtcg ttattttgtg ttgatggggc atctgtcacc gctggacggc
    18781 atcggtccgg atgatatcgg gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc
    18841 actgaagtga tcctcgccac caatcccacg gttgaaggtg aagctaccgc taactacatt
    18901 gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt
    18961 ggcggcgaac tggaaatggt cgatggcacc acgctgtcac actccctcgc cgggcgtcat
    19021 aagattcgtt tttaagcaaa cgagagcagg agcacctgct ctcgcttgaa attattctcc
    19081 cttgtcccca tctctccctc atcctgtttt taaccttaaa atggcattat tgaggtagac
    19141 ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct
    19201 gatgatccat tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc
    19261 ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct aacccggacc tgtacgaagg
    19321 tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc tgaccatctc
    19381 cgataacggc gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa
    19441 atccggtacc aaatcattcc tcgaatccct gggttctgac caagcgaaag acagccagct
    19501 gatcggtcag tttggtgttg gtttctactc tgcgtttatc gtggccgaca aagtgaccgt
    19561 gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga
    19621 aggtgaatat accgttgccg atatcaccaa agaagatcgt ggtactgaaa tcaccctgca
    19681 tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc gtgcgttcca tcatcagcaa
    19741 atactccgac catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga aagatggcga
    19801 aaccgttatc tcctgggcaa aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc
    19861 ggaaatcacc gatgaagagt ataaagagtt ctacaaacac atcgcccacg actttaacga
    19921 tccgctgacc tggagccaca accgcgttga aggtaagcag gagtacacca gcctgctgta
    19981 catcccgtcc caggctccgt gggatatgtg gaaccgtgat cataaacacg gcctgaaact
    20041 gtacgttcaa cgcgtgttta tcatggacga cgctgaacag ttcatgccga actatctgcg
    20101 cttcgtgcgt ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct
    20161 ccaggacagc acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat
    20221 gctggaaaaa ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg
    20281 cctggtactg aaagaaggtc cggcggaaga tttcgctaac caggaagcga tcgccaaact
    20341 gctgcgtttt gcttctactt ataccgattc ttctgcgcag accgtgtctc tggaagacta
    20401 cgtttcccgc atgaaagaag ggcaggagaa aatctactac atcactgcag acagctatgc
    20461 ggcagcgaag agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct
    20521 gctttccgac cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc
    20581 gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg aagttgatga
    20641 gagcgcgaaa gaagcggaga aagcactgac tccgttcatc gaccgcgtga aagctctgct
    20701 cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgatcgt
    20761 ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg cggcgggcca
    20821 gaaagtgcca gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg
    20881 tgcggcagat actgaagatg aagccaagtt cagcgagtgg gtagaactgc tgctggatca
    20941 ggcgctgctg gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa
    21001 tcagctgctg gtttcctgat gtcatgccgg atgacctttg tgtcatccgg catttttctt
    21061 ttcatcatct gtactttccg caaattatct cgccattaac cgtttcagcc tcaggtgcct
    21121 ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa aatcgacaca
    21181 ttttaagggg attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg
    21241 gactcaggct cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat
    21301 gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga
    21361 tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca
    21421 ggaagactgc cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga
    21481 cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga
    21541 actgattgtt gatcgtatcg taggccgccg cgttcatgcg ccgtctggtc gtgtttatca
    21601 cgttaaattc aatccgccga aagtagaagg caaagacgac gttaccggtg aagaactgac
    21661 tacccgtaaa gacgatcagg aagaaaccgt gcgtaaacgt ctggttgaat accatcagat
    21721 gactgcaccg ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc
    21781 gaaagttgac ggtaccaagc aggttgctga agttcgcgct gatctggaaa aaatcctcgg
    21841 ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc ttatccacct caggcaatac
    21901 ctctcacagc aattagttct tcttcctcac ttttccgcta caattatcaa caagttgaat
    21961 cgataagagg cggtaatgcg tcagactaaa accggtatcc tgctggcaaa cctgggtacg
    22021 cccgatgccc ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga
    22081 cgcgtggttg atacctcacg gttgttatgg tggccgttgc tgcgcggcgt gattttgccg
    22141 ctgcgctcgc cgcgtgtggc gaagctgtac gcctctgtct ggatggaaga cggctcgccg
    22201 ctgatggttt acagccgcca gcaacagcag gcgttggcac aacgtttacc ggatacgcca
    22261 gtggcgctgg gcatgagcta cggctcgcca tcactggaaa gctccgtcga tgaactcctg
    22321 gcagagcatg tagatcatat tgtggtgctg ccgctttatc cgcaattctc ctgttctacg
    22381 gtcggtgcgg tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg
    22441 atatcgttta ttcgtgatta cgccgataac cacgattaca ttaatgcact ggcgaacagc
    22501 gtacgcgctt cttttgccaa acatggcgaa ccggatctgc tgctgctctc ttatcatggc
    22561 attccccagc gttatgcaga tgaaggcgat gattacccgc aacgttgccg cacaacgact
    22621 cgcgaactgg cttccgcact ggggatggta ccggaaaaag tgatgatgac ctttcagtcg
    22681 cgctttggcc gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga
    22741 gaaaaaggcg taggtcatat acaggtgatg tgcccgggct ttgctgcgga ttgtctggag
    22801 acgctggaag agattgccga gcaaaaccgt gaggtcttcc tcggtgcggg cgggaaaaaa
    22861 tatgaatata ttccagcgct taatgccacg ccggaacata tcgaaatgat ggctaatctt
    22921 gttgccgcgt atcgctaaag ctgagcggta aagaactgag cgccgtcgcg aagagcctcg
    22981 tcggcggttt tcatcatccg tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt
    23041 ttgaactcac agggctgctg atgcgccgct aacgtctggt aaagcagacg gctgtcatcc
    23101 agcagcggat cgaactccgc cccggcaata aaacagggcg gaacttcgcg agtgagatca
    23161 ttattaaaca gacagtaata cggtgactcg cggtccgcgt cgttgcttaa atacgcctct
    23221 tcatacatct gcaaatcctg ttgcgttaag ccatcccaga caccgcccaa cagacgacga
    23281 gtcacggaat cccgtaatcc gtaaagccca taccacagca aaacacccgc aattttaccg
    23341 caatcgatct gtttatcacg caaccacaac gcactggcga gcgccagcat ggcacctgcg
    23401 gaatcaccgg caaagccaat gcgggacata ttgatttgat aatcctccgc ctgctggtgg
    23461 aaataacaac aagcagccac aatttcctct atcgcttgtg gaaaacgcgc ttcaggtgaa
    23521 agggtgtaat taataccaat caccgtacat tggctgtagc ttgccagcag gcgcatgatg
    23581 cgatcgtggg tatcgagatt gccgagaata aaaccgcctc catgcaaata aaatagcgtc
    23641 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    23701 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    23761 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    23821 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    23881 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    23941 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    24001 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    24061 aaacgtaaat ccaaacatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    24121 cagccagaaa atgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    24181 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    24241 ctggtgattg aggatgacgt agccgaagtg ctttatcagg agctaaaaca gaaaaacctg
    24301 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    24361 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    24421 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    24481 gatggtccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    24541 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    24601 ggagcctcgg cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg
    24661 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    24721 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    24781 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    24841 gatccgttgt tggcatctga caaggcgctg gactgggtag agctggtgct gtgcaccgcc
    24901 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    24961 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    25021 cgccacaagg attgccagaa tccgctgcgt gtctattcgc acattgcgcc gtacatgggc
    25081 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    25141 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    25201 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    25261 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    25321 agccaggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    25381 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    25441 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacttc
    25501 acccgccggt ggcgtttcca gcagttccag catagtacgg gcaatttcac gttcacccat
    25561 cactacctga ttcgcgccac gttcggtgat atacgtcact tcatcgtcat aatgggcgcg
    25621 ggcgataatc tcaatatccg ggtttttcgc gcgggcagac gccacaatct cacccgcttc
    25681 ataaccgttg gggatcgtca ggatcagcca ttttgcacat tccagatgtg ccagttgcat
    25741 aatttcttcg ttcgctgcat tgcccaatac agcgcggacc ccacgctctc gcagctcatc
    25801 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    25861 ccccagcagg ctgcctacac gaccgtaacc caccaatagc gcatggttgc aaatatccac
    25921 tgggatctgt ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    25981 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    26041 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    26101 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    26161 cgtacgttgg gagtgaccaa acagacggac aaggaacagg gccgcgaccg atttaccaaa
    26221 cagaataatt gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    26281 aaacaacatc ccgactgaga caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt
    26341 atcgtgggcg gcacggtgac tcaattcaga ctcgttcagt accatcccgg caaagaacgc
    26401 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    26461 cgccagcact gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat
    26521 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcaccttgcc
    26581 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    26641 tcctgccact gcgggcagca acaccagcgt cagaaccatc accaggtctt ccacaatcaa
    26701 ccaaccgatg gcgatttgcc cacgctgact gtctattaat tgccgttctt caagtgcgcg
    26761 cagtaacacc acggtactgg cggtggaaag acataagccg aacacgatac cggtcattaa
    26821 cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg ctatctgggc
    26881 gatcgcgccg ggaatggcga tggactttac cgccatcaaa tccttgagcg aaaaatgcaa
    26941 accgacgccg aacatcagca aaataacgcc cagttcagcc agttccggcg caagcttggt
    27001 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    27061 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    27121 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    27181 ttattgtcca tttttggctg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    27241 ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    27301 agcacggttc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    27361 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg
    27421 caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat agactaactc
    27481 gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc ccatgccgaa
    27541 agcaaaaccg aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc
    27601 gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac
    27661 ccccgtccag tgcaggcggg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat
    27721 agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt
    27781 acctgccgca acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa
    27841 tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata
    27901 tttcgagaaa atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag
    27961 tggattgatt atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca
    28021 acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac
    28081 gttgcctttg ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc
    28141 aaagttgccg ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat
    28201 acgggctacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc
    28261 tgccagcaga actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt
    28321 aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac
    28381 cactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat
    28441 aaatgtcaga gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat
    28501 catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc
    28561 cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca ttgccatagg
    28621 aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac
    28681 taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc
    28741 tggatgcaga tttatcttca ccggacgcag acttgtctat gatgttgcgt catactattt
    28801 ttcaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc
    28861 gttagcgctg ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga
    28921 taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa
    28981 tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga
    29041 ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt
    29101 gcccgagtct gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta
    29161 tgacgcgatg gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca
    29221 ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga
    29281 gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg
    29341 gctgacaacc gatgacacag caaaaattgg taacctggaa tacttcactg atatcgaatt
    29401 tcgtaagccc gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc
    29461 agacattatt atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa
    29521 cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt
    29581 cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta
    29641 cgtgccgggt acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca
    29701 tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat
    29761 ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa
    29821 aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt
    29881 atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggca aaaccaatgg
    29941 tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat
    30001 tctggcagcc caaatggatc gcactggtgc cgactttgcg gtgatgagcg gaggcggaat
    30061 tcgtgattct atcgaagcag gcgatatcag ctataaaaac gtgctgaaag tgcagccatt
    30121 cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgattgatt acctgaccgc
    30181 cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt
    30241 ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa
    30301 aacttaccgt atggcgacat taaacttcaa tgccaccggc ggtgatggat atccgcgcct
    30361 tgataacaaa ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta
    30421 tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg
    30481 gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa tgcttaatcg cggcgggcga
    30541 tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt tcgatatcca
    30601 gtccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa
    30661 tcgttggcag acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg
    30721 aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta
    30781 cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat
    30841 caccgttcac tgccaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt
    30901 tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa
    30961 acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt
    31021 aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc
    31081 ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag
    31141 tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc
    31201 atttttttag cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac
    31261 cgcgaccaca tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg
    31321 ctgatgcatc agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt
    31381 attttgcggc ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt
    31441 atgccagtgg gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa
    31501 catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc
    31561 cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg
    31621 cgtcgcctga agaaccatcg cgatttgcca cagcggttgg gtagaaaaga gtgagggaga
    31681 aatgcccatc tcctggctaa tgtgctgcag gttttggagt tgctcctcgc taatgcgctc
    31741 ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc
    31801 ctcgacgatc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc
    31861 catatcgtgg ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag
    31921 ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt
    31981 tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg
    32041 agggatggcg caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc
    32101 ttgcatcgct attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac
    32161 cgacgacacc cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca
    32221 atgagttagc agagttgctg catgttcatc gtaatcgcgt cagtgcactg atcaataaca
    32281 atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg
    32341 attttaggct aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca
    32401 cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc
    32461 gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt
    32521 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    32581 gcctacaaac ctgtgcctga accgtaggcc ggataagacg tttacgccgc atccggcagt
    32641 catgcgtcga tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    32701 cttcggttta aaccgcagca accggttggc gttacttact acggtaattg acgagagcgc
    32761 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    32821 ggcggcgacc ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat
    32881 gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg
    32941 catcagggta atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc
    33001 gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg
    33061 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc
    33121 aatcacttca tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc
    33181 ggtcaacatc accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
    33241 actacgcaac ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag
    33301 cacaggcgtt gctccttgcg atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc
    33361 gacttgctga tcatttaaca acgcctgatt acccagcaat aacgcatgac cttctgcttc
    33421 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc
    33481 gcctgcttta tcaaggatcg ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc
    33541 cgccagacgc agcgcctgcg cttcgtcaac atcagcaaat gttttcactg cgacaacctg
    33601 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    33661 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    33721 ggaaataatc gacatcggcg tcgccagtcc cagcgcacac gggcaggcga taatcagcac
    33781 cgtagtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    33841 tgccgcactg acaagcgcaa taaccactac taccggcaca aatatggctg agattttatc
    33901 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct
    33961 tgatagcgtg gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac
    34021 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggattg gttcgcccgt
    34081 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg
    34141 atcgccggtc gtcaggcgca gcaacatacc tggctgcact tctgccagag gcacgctttt
    34201 ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
    34261 tgccttagaa gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc
    34321 gataatcatc gcactggctt catagtaaag atgtcgcgct tccatcggga accactgcgg
    34381 ccacaggttg acgctcatcg aatagagcca cgccacgccc gttcccagcg ccaccagcgt
    34441 atccatcgtc gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgct
    34501 ggcgaaaacc atcaccgcca gggttatcag gccgataacc aaccacaggc tgcggttgtc
    34561 ggcggtgact atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccgc
    34621 cagtgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
    34681 gcgttcgcgg cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    34741 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    34801 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    34861 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    34921 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    34981 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    35041 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    35101 ttgttccaca tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca
    35161 ggacaggccg tccaggttca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    35221 tttgactggc tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa
    35281 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
    35341 catttatttc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    35401 gaaaatcggc actggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    35461 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    35521 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg
    35581 gcggcagtgg ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac
    35641 cgctttgcac tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc
    35701 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    35761 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
    35821 attgccacca ccagcctgat taacgctgaa aacgccgaac aacgctggca gcgaatttta
    35881 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    35941 gaacaaacaa ccaacttcca taatcgggcc attgcctggc tgctgtactc cgccggatat
    36001 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc
    36061 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    36121 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    36181 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
    36241 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    36301 ccaccgctgg acgaagaagg caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct
    36361 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    36421 gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg
    36481 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctggggcg
    36541 cctttgcttt tggcggtatt gtggctatgt tttccggtta tgcctatgcg cgtctggggg
    36601 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    36661 tcttttcgct agcgctctcg ttgctatacc tgttgacgct ggcggtgagc atcgccatgg
    36721 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    36781 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    36841 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    36901 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    36961 cccccagctc cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct
    37021 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    37081 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    37141 ttagcgatgt atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg
    37201 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    37261 cggcttcagg cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    37321 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    37381 atatcatcgt cgtggtgttg ataatgctga tgacggcggc gctaaattta gactcactcg
    37441 ccagcgttgc cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc
    37501 gcctgcgtca tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt
    37561 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    37621 ggattattgg tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa
    37681 ccgtataaca cctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc
    37741 gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc
    37801 gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc
    37861 attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat
    37921 cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg
    37981 ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac
    38041 agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag
    38101 gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac
    38161 agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga
    38221 ttaagacgag acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg
    38281 cgtacctgca ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
    38341 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
    38401 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
    38461 tttccaccac agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc
    38521 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
    38581 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
    38641 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg
    38701 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
    38761 ggcatcatca ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat
    38821 ttttgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc
    38881 attttggtgg cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac
    38941 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
    39001 aggatttccg cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc
    39061 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
    39121 tcaatacggg tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg
    39181 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg
    39241 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcatag
    39301 gcagcgcgtg gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg
    39361 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
    39421 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
    39481 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
    39541 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
    39601 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
    39661 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
    39721 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
    39781 tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
    39841 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
    39901 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
    39961 aaaccagaaa tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc
    40021 gatacggtat acgataatct gatctttccc tggcagatcc gtgaccagca gcctgacccg
    40081 gccatttttc tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaagaat
    40141 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa cctgcaattt
    40201 atgccgaagg ttttattgct ggatgaaata accagtgcgc tggatgaaag taataaacat
    40261 aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
    40321 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
    40381 ggagaaatgc aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact
    40441 ggcattaatg ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga
    40501 gaaagatatt ctatggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt
    40561 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
    40621 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
    40681 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
    40741 aggggcgatt gaatttatcc cgatgcaggt gatccctgtc gccgggatga ttgccggtaa
    40801 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
    40861 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
    40921 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
    40981 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
    41041 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
    41101 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
    41161 gactcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
    41221 gttcatgtgc cgaacaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
    41281 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
    41341 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc
    41401 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
    41461 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt
    41521 cagcagttcg atttgcgcta ccagcccctg gtagcgggtg tcctgatcct gcaacggaat
    41581 ggttttcagc accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag
    41641 cagcaggccg atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag
    41701 ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
    41761 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
    41821 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
    41881 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
    41941 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
    42001 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
    42061 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
    42121 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
    42181 catcacgcgc ccaggcagca ggcgctcgag caccattact gcccaggtca ccagcgttac
    42241 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc
    42301 ttccggtccc aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg
    42361 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
    42421 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
    42481 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
    42541 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
    42601 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
    42661 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
    42721 cagggcgatc acctcgtctg ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa
    42781 cacgccggta aatcccatgt tgttcatacg ctcaacatca ttcggttttc ggcaacctgc
    42841 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
    42901 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
    42961 atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct
    43021 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
    43081 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
    43141 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
    43201 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
    43261 gctgtggctg aaaaccacgc aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg
    43321 ctgatgctgt ttgagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
    43381 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    43441 ggccgcgctg gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt
    43501 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    43561 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    43621 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    43681 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    43741 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    43801 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    43861 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    43921 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    43981 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    44041 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    44101 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    44161 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    44221 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    44281 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    44341 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    44401 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    44461 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    44521 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    44581 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    44641 ttaacctgaa aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag
    44701 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    44761 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    44821 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    44881 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    44941 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    45001 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    45061 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    45121 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    45181 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    45241 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    45301 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    45361 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    45421 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    45481 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
    45541 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    45601 agctttcggc attttcgctc tcctttatgc tgctggcact gctgctggtg ctgcgtggcg
    45661 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaacgac tttttaatta
    45721 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45781 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaatgat aagccgacag
    45841 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45901 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45961 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    46021 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46081 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46141 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46201 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46261 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46321 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46381 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgatgtt cgccatgctt
    46441 gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc agtgttgcag
    46501 gcgaaagtgt ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc
    46561 agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt
    46621 aaggcgctgt tcaggcagtt tgtggggtga tgacaggtgt tagattgcct gatgcgctac
    46681 gcttatcagg cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga
    46741 taaggcgttt acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc
    46801 aactgtcatg tcggtttttt taaaggccga aggaggtaaa ccttcggcct tagctacgtt
    46861 tcctcagtta ttcaataaac tgacggatca gttccagcag cgcgattagc gccttaagaa
    46921 cgatgagaac tttatcgaga aaagttttca tccgttgctc ccttaaggga gaaagacaat
    46981 cacagcttac ccttacgttg cctataacta tgattttctt catgtgctag atgaggactg
    47041 tctctctcca aattagagca ccagaatcag caccacctgt atcctgacca ggacttaaaa
    47101 gtacttttcc gcactgcggt aatcccttga ggcagtatca ctactagcgt cgggattgta
    47161 tcgccaacat agctgtttga atatgtaggc atcctgactt ataattcact tgctagatga
    47221 ggactgttta atttctgccc ggaaccacca agtcttgggc ggaaaaaata aaaaaaccga
    47281 ctgtcatgtc ggttttttta tatctgtatt ctggtgcaac cattttgttc tcacgacaat
    47341 cgccgtttca gaataccgcc catcactgcg ccttaaccca taccgccact tccgcccact
    47401 caccgcgaaa gacaactttt tccgcttttt tctcaagctg atagcgatac attgggtcgt
    47461 aatattcttt aagtaacggc accagccagg ccagatgacc gtcggtgctg ccggtggtga
    47521 gttgcgttgt cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca
    47581 gccgacgctt aatcgccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct
    47641 gttcgtcgcc ataagcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcattca
    47701 ggcgttcaag acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc
    47761 gctcgcgcag acattccggc aggtgattcg aaccgatcat ccggctttca tcttccagca
    47821 cccacaggcg caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt
    47881 caaaactcgc ctggctaagt tgtggttgta acgtgcgacc aaacgccgaa ccgcgatgac
    47941 gcgccagccc ttccagatca acaccgtttg gctgttgttg cactaatagc gttttaccgc
    48001 tgccggtaca accgccaatc agcactatcg gtttttgtgc cagttcaatg gtcgcctgaa
    48061 tcgcggtctg gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctt
    48121 catgcagcca gcgttgcaca atatgtgaac gttgaccgcc acgggcgcag cagagaatac
    48181 cttgcggatt ttgcaggcac gctgcccgcc aggcgtacat acgctgctga cgaatttcac
    48241 ccgccaccag tttatgcccc agcgccagcg ctgcgtctga gccttgctgt ttatagcagg
    48301 tgccaacgga ggcgcgttca tcgttatcca ttaacggcag attgatagcg gcgggcattg
    48361 cgccttgctc aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca
    48421 gggcacgata gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca
    48481 gcttgcgcgc tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtggggt
    48541 gccgaaaatt tctaaaaatc cgctgatttc cggcctgtgc tgggtaaaca aggtcacaat
    48601 atcttctacc gctttgcagc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg
    48661 gcgcatcgtt gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag
    48721 tgattttggc aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat
    48781 atcgggaaca ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt
    48841 gccggcgctg tcttcaatat tgaccgccgg aaagcgacgc aactgcgctt ctgttagcgg
    48901 ctcttcaacg ttcgccagcg gatgatccgc cgccatgaca aatcgccatt gcaccgatcc
    48961 taagggatca agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag
    49021 cgaaaaacct tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg
    49081 aaactgggta aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg
    49141 gttatagagc aggttgttga tgacaatatt cacctggcgt tccacgccat cattcacctg
    49201 ttgcagctcg ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag
    49261 atgctcgcca gccgctgtca acgttacgct acgagtcgta cggaaaaaca gcgctactcc
    49321 ggtattctct tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg
    49381 ttctgccgct tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc
    49441 tggatcgaac atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc
    49501 tctgatttat cagtattttt acatgataat cctgttcaat ttgtggacta aatctagttt
    49561 tggaaaaaca ttccaacttt tgtattaatg ttgttctctt aaggttttag attgcctgtt
    49621 attgaaacca agctgaccgg tcggcggtgg ttgaacggaa ttatgttaca aggacaaaaa
    49681 gatgaaactt caggtattac cgttaagtca ggaagccttt agtgcttatg gcgacgtaat
    49741 cgaaacgcag caacgggatt ttttccatat taacaatggc ctggtggagc gttaccacga
    49801 tttggcgctg gttgagattc ttgagcaaga ccgtacgctt atcagcatta accgcgcgca
    49861 accggcgaat ctgccgctga ccattcacga actcgaacgt catccgctgg gcactcaggc
    49921 ctttatccca atgaaaggtg aagtgtttgt ggtggtcgtg gcgttaggcg acgacaaacc
    49981 agacctgtca acgcagcggg cgtttatcac caacggtgaa cagggggtga attaccatcg
    50041 taacgtctgg catcacccac ttttcgcctg gcagcgcgtc accgattttc tgaccatcga
    50101 tcgcggcggc agtgacaact gtgatgttga aagtattcct gaacaggaac tctgttttgc
    50161 gtgacgcctg caaccgactt gcataagata aactaattgt tcattgttta tgctcacttg
    50221 taggtcggag ttaacgtagg tatgacggaa gttagacggc gcggcaggcc aggacaggcg
    50281 gagcctgtgg cacagaaggg cgcacaggcg ttagagcggg gaattgcgat tctgcaatat
    50341 ctggaaaaaa gtgggggaag ttcgtcggtt agcgatattt ctctcaatct ggatttgccg
    50401 ctctccacga cctttcgctt gctgaaggtt ttacaagcag cggattttgt ctatcaggac
    50461 agtcaattag gctggtggca tataggatta ggtgtcttta acgtcggtgc ggcgtacatc
    50521 cataaccgcg atgtcctctc cgtcgccggg ccgtttatgc gccgcctgat gttactttcc
    50581 ggcgaaacgg tcaatgtcgc gatccgtaac ggcaatgaag cggtattaat tggtcagtta
    50641 gagtgtaaat cgatggtcag gatgtgtgcg ccactgggca gtcgtctgcc gctgcatgct
    50701 tccggtgcgg gcaaagcgct gctttatccg ctggcggaag aggagttgat gagcatcatt
    50761 ctgcaaaccg gtttgcagca gtttacgcca actacgcttg tggatatgcc caccttgctg
    50821 aaggacctgg aacaagcgcg tgaactgggc tataccgtag ataaagaaga gcatgttgta
    50881 ggtctgaatt gcatagcttc agcaatttac gatgatgtcg gtagagttgt tgccgctatc
    50941 tccatctccg ggccttcatc aagactgaca gaagatcgtt ttgtcagtca gggtgagctg
    51001 gtcagagaca ccgcccgcga tatcagcacg gcgttgggac tgaaagcaca tccatagtgt
    51061 ctgtcgcatc ccgctctgcg gagcgggttt ttttgacaaa atttgaaact tggagaattt
    51121 ttccaattaa tagaggtagg aataaaatgg caaaaatgag agccgttgac gcggcaatgt
    51181 atgtgctgga gaaagaaggt atcaccaccg ccttcggcgt tccgggagct gcaattaatc
    51241 cgttctactc agcgatgcgt aagcacggcg gtattcgtca cattctggcg cgtcatgtgg
    51301 aaggtgtttc gcacatggcg gaaggttata cccgcgcaac ggcagggaat atcggcgtat
    51361 gtctggggac ttccggtcct gcgggcacgg acatgatcac cgcgctctat tccgcttctg
    51421 ctgattccat tcctattctg tgcattaccg gccaggcacc gcgcgcccgt ctgcataaag
    51481 aagattttca ggccgtagat attgaagcaa ttgctaaacc ggtcagcaaa atggcggtta
    51541 cagttcgtga agcggcgctg gtgcctcgcg tgctgcaaca ggcatttcac ctgatgcgtt
    51601 ctggtcgtcc gggtccggta ctggtggatt taccgttcga cgttcaggtt gcggaaatcg
    51661 agtttgatcc tgacatgtac gaaccgctgc cggtctacaa acctgctgcc agccgtatgc
    51721 agatcgaaaa agctgtagaa atgttaatcc aggccgaacg tccggtgatt gttgccgggg
    51781 gcggggtaat caatgctgac gcagctgcac tgttacaaca gtttgctgaa ctgaccagcg
    51841 ttccggtgat cccaacgctg atgggctggg gctgtatccc ggacgatcat gaactgatgg
    51901 ccgggatggt cggtctgcaa accgcgcatc gttacggtaa cgcaacgttg ctggcgtccg
    51961 acatggtgtt tggtatcggt aaccgttttg ctaaccgtca taccggttcg gtagagaaat
    52021 acaccgaagg gcgcaaaatc gttcatattg atattgagcc gacgcaaatt ggtcgcgtgc
    52081 tgtgtccgga tctcggcatt gtctctgatg ctaaagcggc gctgacactg ctggttgaag
    52141 tggcgcagga gatgcaaaaa gcgggtcgtc tgccgtgtcg taaagaatgg gtcgccgact
    52201 gccagcagcg taaacgcact ttgctgcgca aaacccactt cgacaacgtg ccggtgaaac
    52261 cgcagcgcgt gtatgaagag atgaacaaag cctttggtcg cgatgtttgt tatgtcacca
    52321 ctattggtct gtcacaaatc gctgcggcac aaatgctgca tgtctttaaa gaccgccact
    52381 ggatcaactg tggtcaggct ggtccgttag gctggacgat tccggcggcg ctgggggttt
    52441 gtgccgctga tccgaaacac aatgtggtgg cgatttctgg cgactttgac ttccagttcc
    52501 tgattgaaga gttagccgtt ggcgcgcagt tcaacattcc gtacatccat gtgctggtga
    52561 ataacgccta tctcggcctg attcgtcagt cacaacgcgc ttttgacatg gactactgcg
    52621 tgcaactcgc tttcgagaat atcaactcca gtgaagtgaa tggctacggt gttgaccacg
    52681 taaaagtagc ggaaggttta ggttgtaaag ctattcgggt cttcaaaccg gaagatattg
    52741 cgccagcctt tgaacaggcg aaagccttaa tggcgcaata tcgggtaccg gtagtcgtgg
    52801 aagttattct cgagcgtgtg accaatattt cgatgggcag cgaactggat aacgtcatgg
    52861 aatttgaaga tatcgccgat aacgcagcgg atgcaccgac tgaaacctgc ttcatgcact
    52921 atgaataagg gagataaatg atgttacgtt tctctgctaa tttatcgatg ttatttggtg
    52981 aatatgattt tctcgcccgt tttgagaaag ctgcgcagtg tggttttcgc ggcgttgaat
    53041 ttatgtttcc ttatgactac gacattgaag aattaaaaca ggtactggcg agtaataaac
    53101 tcgaacatac gctgcacaat ttaccggcag gtgactgggc ggcgggcgag cgcggtattg
    53161 cctgtattcc tggccgtgaa gaagagtttc gggatggcgt agcagcagcg att
//
LOCUS       DAJTOW010000015        53195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTOW010000015 DAJTOW010000000
VERSION     DAJTOW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.015"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO244_000988"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO244_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5662905.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO244_000989"
     CDS             complement(1151..1237)
                     /locus_tag="OO244_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5662906.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO244_000990"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO244_000990"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5662907.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO244_000991"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO244_000991"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5662908.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO244_000992"
     CDS             3586..3630
                     /locus_tag="OO244_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662909.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO244_000993"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO244_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5662910.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OO244_000994"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OO244_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5662911.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OO244_000995"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OO244_000995"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5662912.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OO244_000996"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OO244_000996"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5662913.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OO244_000997"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OO244_000997"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5662914.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OO244_000998"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OO244_000998"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5662915.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO244_000999"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO244_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5662916.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO244_001000"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO244_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5662917.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="OO244_001001"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="OO244_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5662918.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="OO244_001002"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="OO244_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5662919.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="OO244_001003"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="OO244_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5662920.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="OO244_001004"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="OO244_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5662921.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="OO244_001005"
     CDS             complement(16982..17746)
                     /locus_tag="OO244_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5662922.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO244_001006"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO244_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5662923.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="OO244_001007"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="OO244_001007"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5662924.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="OO244_001008"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="OO244_001008"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5662925.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVA
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="OO244_001009"
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="OO244_001009"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5662926.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="OO244_001010"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="OO244_001010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5662927.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="OO244_001011"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="OO244_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5662928.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="OO244_001012"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="OO244_001012"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5662929.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO244_001013"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO244_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5662930.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="OO244_001014"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="OO244_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5662931.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="OO244_001015"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="OO244_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5662932.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="OO244_001016"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="OO244_001016"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5662933.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="OO244_001017"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="OO244_001017"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5662934.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="OO244_001018"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="OO244_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5662935.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="OO244_001019"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="OO244_001019"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5662936.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO244_001020"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO244_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5662937.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO244_001021"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO244_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5662938.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO244_001022"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO244_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5662939.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41816)
                     /locus_tag="OO244_001023"
     CDS             complement(40422..41816)
                     /locus_tag="OO244_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5662940.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPASPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO244_001024"
     CDS             complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO244_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5662941.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42159..43445)
                     /locus_tag="OO244_001025"
     CDS             complement(42159..43445)
                     /locus_tag="OO244_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5662942.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGVQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43496..44437)
                     /locus_tag="OO244_001026"
                     /pseudo
     CDS             complement(43496..44437)
                     /locus_tag="OO244_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44452..45222)
                     /locus_tag="OO244_001027"
     CDS             complement(44452..45222)
                     /locus_tag="OO244_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5662943.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45578..47092
                     /gene="caiT"
                     /locus_tag="OO244_001028"
     CDS             45578..47092
                     /gene="caiT"
                     /locus_tag="OO244_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5662944.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47123..48265
                     /gene="caiA"
                     /locus_tag="OO244_001029"
     CDS             47123..48265
                     /gene="caiA"
                     /locus_tag="OO244_001029"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5662945.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48394..49611
                     /gene="caiB"
                     /locus_tag="OO244_001030"
     CDS             48394..49611
                     /gene="caiB"
                     /locus_tag="OO244_001030"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5662946.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49685..51238
                     /gene="caiC"
                     /locus_tag="OO244_001031"
     CDS             49685..51238
                     /gene="caiC"
                     /locus_tag="OO244_001031"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5662947.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51347..52132
                     /gene="caiD"
                     /locus_tag="OO244_001032"
     CDS             51347..52132
                     /gene="caiD"
                     /locus_tag="OO244_001032"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5662948.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52138..52728
                     /gene="caiE"
                     /locus_tag="OO244_001033"
     CDS             52138..52728
                     /gene="caiE"
                     /locus_tag="OO244_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5662949.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52814..>53195)
                     /gene="caiF"
                     /locus_tag="OO244_001034"
     CDS             complement(52814..>53195)
                     /gene="caiF"
                     /locus_tag="OO244_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5662950.1"
                     /translation="VEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNTLTYIL
                     SEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVRKTPRI
                     PAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT        12975 a        14386 c        13814 g        12020 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgactga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa
     5041 ttcttaacat taattgttca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccaag
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgctaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacag aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccaactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacaccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg gcgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    22321 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
    24661 gcgataaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgctg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtt
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccag cgccagcgtc
    40861 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    40921 ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt
    40981 ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac
    41041 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    41101 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    41161 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    41221 cagcggggag atgctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc
    41281 gcgctaccca gcatccaacg ccaaccacct tccacatcat aaagccagta gccgaccaga
    41341 tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    41401 tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg
    41461 acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc
    41521 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    41581 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    41641 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    41701 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    41761 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    41821 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    41881 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    41941 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    42001 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac
    42061 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    42121 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    42181 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    42241 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    42301 agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac
    42361 atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc
    42421 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    42481 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    42541 tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc
    42601 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc
    42661 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    42721 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    42781 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    42841 aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    42901 gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc
    42961 accccatcag caaggagcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg
    43021 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    43081 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    43141 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    43201 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg
    43261 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    43321 gcacctgcgg aattgccacg ctcgataact aacacttgca ccccttcgcg ggcgagcacc
    43381 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    43441 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    43501 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    43561 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg
    43621 cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag
    43681 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    43741 ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    43801 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt
    43861 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    43921 gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    43981 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    44041 tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg
    44101 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    44161 tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt
    44221 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    44281 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    44341 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg
    44401 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    44461 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    44521 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc
    44581 gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    44641 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    44701 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    44761 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    44821 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag
    44881 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    44941 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    45001 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    45061 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    45121 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    45181 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    45241 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    45301 aataaaactt taatcaccaa tattgaaagt cagatgctat ttttttaccc acaaaagaat
    45361 ataacaaact gattaataac caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa
    45421 aatgtgatac caatcacaga atacagctta ttgaataccc attatgagtt agccattaac
    45481 gcgtccacga ggttaataat aattatatta aatgttaaca aaaataaaac aaacgggaac
    45541 gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa agagaaaaac
    45601 gggaatagaa ccgaaggttt tctttccgcc attaataatc gtcggcatac tttgttggct
    45661 tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca gttacgtcac
    45721 caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg gttggttctg
    45781 gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag aatttagcac
    45841 cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac tgttctgggg
    45901 atcgattgag atctactact acatctccac cccgccgttt ggcttagaac cgaactcgac
    45961 aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc tgccgtgggc
    46021 cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca aaatggaagt
    46081 gattcgcccc agctcgacac tggtgccgct ggtaggtgaa aaacacgcca aagggttgtt
    46141 cggtaccatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct
    46201 gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac
    46261 cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca tttgcgtcgc
    46321 ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct
    46381 gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact acttcaccga
    46441 ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg atcccatcgc
    46501 taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg tgatttacgc
    46561 catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg
    46621 cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg tgctcggtag
    46681 taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga tcgaacagta
    46741 cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca ccgccaccat
    46801 gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg cctgctctta
    46861 taccctggcg atgtctactt gccgcgaagt acgcgatggt gaagaaccac cgctgctggt
    46921 gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg cgctcggcgg
    46981 cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct tcgtcaacat
    47041 tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt aattaacccc
    47101 caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg aactgtttgt
    47161 cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg ccgagtgcga
    47221 ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg gtatcgacag
    47281 tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc tcgccgccgt
    47341 gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagc tgccgggcgg
    47401 gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta tggctttccg
    47461 cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt
    47521 gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta atggtagtaa
    47581 gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc gcgacggggc
    47641 ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac cgggcatcaa
    47701 agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa tcacctttga
    47761 cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt
    47821 caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg gtacggcgat
    47881 gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat
    47941 tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat taaactccat
    48001 gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca cctctggcga
    48061 tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat
    48121 gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct tctggcgtga
    48181 cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga cgctgggtcg
    48241 tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc tgacgcgtct
    48301 tatcaggcct acgggagcac gaatgtaagc cggataaggc gtttacgccg catccggcag
    48361 tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat gccgaaattc
    48421 ggaccgctgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc
    48481 ggacaaatgt tcgcagaatg gggcgcggaa gttatttgga tcgagaacgt cgcctgggcc
    48541 gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt gcacgcgctg
    48601 tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc
    48661 gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat taccgatgaa
    48721 gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt tggtcagtac
    48781 ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc cttcagtggt
    48841 tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta taccgccgat
    48901 tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa agtgcgtgaa
    48961 accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg tatgggccag
    49021 tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa
    49081 gatccctact acgccggttg cggtctgtat aaatgtgccg acggctacat cgtgatggaa
    49141 ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc acatctactc
    49201 ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg cccttacggc
    49261 ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc agaagttaaa
    49321 gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa
    49381 agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc
    49441 acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca aatctggcgc
    49501 ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg ctacagcgaa
    49561 aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc
    49621 gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa tggatagagg
    49681 tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc tggcggacgt
    49741 ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta accggtatag
    49801 ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat
    49861 tcgcaaaggc aacaaggttg cactacatct cgacaactgc ccggaattta tcttttgctg
    49921 gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga
    49981 ggaaagcgag tggatcttgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt
    50041 ttatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc acatttgcct
    50101 gacagatgtg gcacttcccg ctgatgatgg agtgagttca tttactcaac tgaaaaatca
    50161 acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct
    50221 cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt acaacctgcg
    50281 cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg tctacatgac
    50341 ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc
    50401 cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg gacaggcgca
    50461 gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt tgatggtgca
    50521 gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt
    50581 gtcggagcag gaaaaagacg cattttgtga acgcttcggc gttcgcttgc tgacgtctta
    50641 tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctagcgata aacgacgctg
    50701 gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg
    50761 cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat
    50821 cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgacggctg
    50881 gctacatacc ggcgataccg gataccgcga cgaagaaggc tttttttatt tcgtcgatcg
    50941 ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat
    51001 catcgccacc catccgaaaa ttcaggacat cgtggttgtg ggtattaaag attcgattcg
    51061 cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga
    51121 attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat
    51181 cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat
    51241 gaaacgtcag ggaactaccc tgccagctaa taatcacgcc ctaaaacaat atgcattttt
    51301 tgcagggatg ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga gtgaatcatt
    51361 acatctgacc cgcaatggat caattctgga aattaccctt gatcgcccaa aagcgaatgc
    51421 tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc
    51481 gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga
    51541 tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggttttgc
    51601 gggattaacc gaaattttca atctcgacaa accagttatc gcagctgtga acggctacgc
    51661 ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc
    51721 cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg gtgtgctgcg
    51781 tctgccgaag atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat
    51841 gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc aggcggaact
    51901 gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc tggcgattgc
    51961 ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta
    52021 tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga
    52081 agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg
    52141 agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc
    52201 agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc
    52261 tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc
    52321 tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg
    52381 catggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc
    52441 gtaattatgg atggcgcggt cattggcgaa gagagcattg ttgccgcaat gagctttgtc
    52501 aaagcaggct ttagtggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta
    52561 cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat
    52621 cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa
    52681 aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg
    52741 cgacctggtt gctggataag atgctacaac atcgtatctg gcaagcagga cgcccggtat
    52801 caacgagata aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
    52861 ggttaagttc cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg ccgactaatt
    52921 tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa
    52981 ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc
    53041 agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
    53101 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
    53161 attcggcgat taacaagtag agtggttttt caaca
//
LOCUS       DAJTOW010000016        52597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTOW010000016 DAJTOW010000000
VERSION     DAJTOW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.016"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..354
                     /locus_tag="OO244_001035"
     CDS             <1..354
                     /locus_tag="OO244_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5662951.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO244_001036"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO244_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5662952.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO244_001037"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO244_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5662953.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSGLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO244_001038"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO244_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5662954.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO244_001039"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO244_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5662955.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO244_001040"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO244_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5662956.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO244_001041"
     CDS             6877..7281
                     /locus_tag="OO244_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5662957.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO244_001042"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO244_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5662958.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFVGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO244_001043"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO244_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5662959.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLPMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO244_001044"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO244_001044"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5662960.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO244_001045"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO244_001045"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5662961.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO244_001046"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO244_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO244_001047"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO244_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5662962.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO244_001048"
     CDS             complement(13133..13429)
                     /locus_tag="OO244_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5662963.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO244_001049"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO244_001049"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5662964.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO244_001050"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO244_001050"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5662965.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGEQEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDNPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO244_001051"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO244_001051"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5662966.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO244_001052"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO244_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5662967.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO244_001053"
     CDS             complement(18686..19372)
                     /locus_tag="OO244_001053"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5662968.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELVLADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO244_001054"
     CDS             complement(19772..19912)
                     /locus_tag="OO244_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662969.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO244_001055"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO244_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5662970.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO244_001056"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO244_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5662971.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO244_001057"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO244_001057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5662972.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO244_001058"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO244_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5662973.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO244_001059"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO244_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5662974.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO244_001060"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO244_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5662975.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO244_001061"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO244_001061"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5662976.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO244_001062"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO244_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO244_001063"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO244_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5662977.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO244_001064"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO244_001064"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5662978.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO244_001065"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO244_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5662979.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO244_001066"
     CDS             complement(31511..31678)
                     /locus_tag="OO244_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5662980.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO244_001067"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO244_001067"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5662981.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO244_001068"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO244_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5662982.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO244_001069"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO244_001069"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5662983.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO244_001070"
     CDS             35470..36114
                     /locus_tag="OO244_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5662984.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO244_001071"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO244_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO244_001072"
     CDS             37189..37452
                     /locus_tag="OO244_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5662985.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO244_001073"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO244_001073"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5662986.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO244_001074"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO244_001074"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5662987.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO244_001075"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO244_001075"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5662988.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO244_001076"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO244_001076"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5662989.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO244_001077"
     CDS             42150..43691
                     /locus_tag="OO244_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5662990.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO244_001078"
     CDS             43663..44526
                     /locus_tag="OO244_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5662991.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO244_001079"
     CDS             complement(44740..45522)
                     /locus_tag="OO244_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5662992.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO244_001080"
     CDS             complement(45519..46592)
                     /locus_tag="OO244_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5662993.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO244_001081"
     CDS             complement(46714..46875)
                     /locus_tag="OO244_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5662994.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO244_001082"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO244_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5662995.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO244_001083"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO244_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5662996.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO244_001084"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO244_001084"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5662997.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO244_001085"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO244_001085"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5662998.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO244_001086"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO244_001086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5662999.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO244_001087"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO244_001087"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5663000.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12525 a        14235 c        13181 g        12656 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaacccg ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttgttgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggg ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgc cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggattg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tcctgctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacac taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttactg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatgg
//
LOCUS       DAJTOW010000017        49607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTOW010000017 DAJTOW010000000
VERSION     DAJTOW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.017"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..1224
                     /locus_tag="OO244_001088"
     CDS             <1..1224
                     /locus_tag="OO244_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5663001.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO244_001089"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO244_001089"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5663002.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTKNRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2716
                     /gene="fumE"
                     /locus_tag="OO244_001090"
                     /pseudo
     CDS             2208..2716
                     /gene="fumE"
                     /locus_tag="OO244_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
     gene            2713..3426
                     /locus_tag="OO244_001091"
     CDS             2713..3426
                     /locus_tag="OO244_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5663003.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3398..4075)
                     /locus_tag="OO244_001092"
     CDS             complement(3398..4075)
                     /locus_tag="OO244_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5663004.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4069..4746)
                     /locus_tag="OO244_001093"
     CDS             complement(4069..4746)
                     /locus_tag="OO244_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5663005.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4734..5117)
                     /locus_tag="OO244_001094"
     CDS             complement(4734..5117)
                     /locus_tag="OO244_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5663006.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5133..5441)
                     /locus_tag="OO244_001095"
     CDS             complement(5133..5441)
                     /locus_tag="OO244_001095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663007.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5442..6020)
                     /locus_tag="OO244_001096"
     CDS             complement(5442..6020)
                     /locus_tag="OO244_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5663008.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6043..6474)
                     /locus_tag="OO244_001097"
                     /pseudo
     CDS             complement(6043..6474)
                     /locus_tag="OO244_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6846..7865
                     /gene="epd"
                     /locus_tag="OO244_001098"
     CDS             6846..7865
                     /gene="epd"
                     /locus_tag="OO244_001098"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5663009.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7915..9078
                     /gene="pgk"
                     /locus_tag="OO244_001099"
     CDS             7915..9078
                     /gene="pgk"
                     /locus_tag="OO244_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5663010.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9293..10372
                     /gene="fbaA"
                     /locus_tag="OO244_001100"
     CDS             9293..10372
                     /gene="fbaA"
                     /locus_tag="OO244_001100"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5663011.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10563..11423
                     /gene="mscS"
                     /locus_tag="OO244_001101"
     CDS             10563..11423
                     /gene="mscS"
                     /locus_tag="OO244_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5663012.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11562..12197
                     /gene="argO"
                     /locus_tag="OO244_001102"
     CDS             11562..12197
                     /gene="argO"
                     /locus_tag="OO244_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5663013.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12290..13030
                     /locus_tag="OO244_001103"
     CDS             12290..13030
                     /locus_tag="OO244_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5663014.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13197..14147
                     /locus_tag="OO244_001104"
     CDS             13197..14147
                     /locus_tag="OO244_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5663015.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14089..15567)
                     /gene="scpC"
                     /locus_tag="OO244_001105"
                     /pseudo
     CDS             complement(14089..15567)
                     /gene="scpC"
                     /locus_tag="OO244_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15591..16376)
                     /gene="scpB"
                     /locus_tag="OO244_001106"
     CDS             complement(15591..16376)
                     /gene="scpB"
                     /locus_tag="OO244_001106"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5663016.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="OO244_001107"
     CDS             complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="OO244_001107"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5663017.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVSSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="OO244_001108"
     CDS             complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="OO244_001108"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5663018.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATPGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVHDVLNLISQHHD"
     gene            complement(19723..20616)
                     /gene="argP"
                     /locus_tag="OO244_001109"
     CDS             complement(19723..20616)
                     /gene="argP"
                     /locus_tag="OO244_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5663019.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20999..21658
                     /gene="rpiA"
                     /locus_tag="OO244_001110"
     CDS             20999..21658
                     /gene="rpiA"
                     /locus_tag="OO244_001110"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5663020.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21914..23146
                     /gene="serA"
                     /locus_tag="OO244_001111"
     CDS             21914..23146
                     /gene="serA"
                     /locus_tag="OO244_001111"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5663021.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23375..23434
                     /gene="ibsC"
                     /locus_tag="OO244_001112"
     CDS             23375..23434
                     /gene="ibsC"
                     /locus_tag="OO244_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5663022.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23767..23826
                     /locus_tag="OO244_001113"
     CDS             23767..23826
                     /locus_tag="OO244_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5663023.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23927..24529)
                     /locus_tag="OO244_001114"
     CDS             complement(23927..24529)
                     /locus_tag="OO244_001114"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5663024.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24550..24733)
                     /gene="ssrS"
                     /locus_tag="OO244_001115"
     ncRNA           complement(24550..24733)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO244_001115"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="OO244_001116"
     CDS             complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="OO244_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5663025.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25272..25850
                     /gene="ygfB"
                     /locus_tag="OO244_001117"
     CDS             25272..25850
                     /gene="ygfB"
                     /locus_tag="OO244_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5663026.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25876..27201
                     /gene="pepP"
                     /locus_tag="OO244_001118"
     CDS             25876..27201
                     /gene="pepP"
                     /locus_tag="OO244_001118"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5663027.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTTEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLNVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27198..28376
                     /gene="ubiH"
                     /locus_tag="OO244_001119"
                     /gene_synonym="visB"
     CDS             27198..28376
                     /gene="ubiH"
                     /locus_tag="OO244_001119"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5663028.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28399..29601
                     /gene="ubiI"
                     /locus_tag="OO244_001120"
                     /gene_synonym="visC"
     CDS             28399..29601
                     /gene="ubiI"
                     /locus_tag="OO244_001120"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5663029.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29675..29758)
                     /locus_tag="OO244_001121"
     CDS             complement(29675..29758)
                     /locus_tag="OO244_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663030.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30049..31143
                     /gene="gcvT"
                     /locus_tag="OO244_001122"
     CDS             30049..31143
                     /gene="gcvT"
                     /locus_tag="OO244_001122"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5663031.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVKGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31167..31556
                     /gene="gcvH"
                     /locus_tag="OO244_001123"
     CDS             31167..31556
                     /gene="gcvH"
                     /locus_tag="OO244_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5663032.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31675..34548
                     /gene="gcvP"
                     /locus_tag="OO244_001124"
     CDS             31675..34548
                     /gene="gcvP"
                     /locus_tag="OO244_001124"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5663033.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34815..35558
                     /locus_tag="OO244_001125"
     CDS             34815..35558
                     /locus_tag="OO244_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5663034.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35615..37048)
                     /gene="bglA"
                     /locus_tag="OO244_001126"
     CDS             complement(35615..37048)
                     /gene="bglA"
                     /locus_tag="OO244_001126"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5663035.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37093..37403
                     /locus_tag="OO244_001127"
                     /pseudo
     CDS             37093..37403
                     /locus_tag="OO244_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37567..38226
                     /locus_tag="OO244_001128"
     CDS             37567..38226
                     /locus_tag="OO244_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5663036.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38422..39402)
                     /gene="ygfZ"
                     /locus_tag="OO244_001129"
     CDS             complement(38422..39402)
                     /gene="ygfZ"
                     /locus_tag="OO244_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5663037.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39645..39911
                     /gene="sdhE"
                     /locus_tag="OO244_001130"
     CDS             39645..39911
                     /gene="sdhE"
                     /locus_tag="OO244_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5663038.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39892..40299
                     /locus_tag="OO244_001131"
     CDS             39892..40299
                     /locus_tag="OO244_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5663039.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40339..40860)
                     /gene="fldB"
                     /locus_tag="OO244_001132"
     CDS             complement(40339..40860)
                     /gene="fldB"
                     /locus_tag="OO244_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5663040.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40972..41868
                     /gene="xerD"
                     /locus_tag="OO244_001133"
     CDS             40972..41868
                     /gene="xerD"
                     /locus_tag="OO244_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5663041.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41893..42603
                     /gene="dsbC"
                     /locus_tag="OO244_001134"
     CDS             41893..42603
                     /gene="dsbC"
                     /locus_tag="OO244_001134"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5663042.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42609..44342
                     /gene="recJ"
                     /locus_tag="OO244_001135"
     CDS             42609..44342
                     /gene="recJ"
                     /locus_tag="OO244_001135"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5663043.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44433..45531
                     /gene="prfB"
                     /locus_tag="OO244_001136"
     CDS             join(44433..44507,44509..45531)
                     /gene="prfB"
                     /locus_tag="OO244_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5663044.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45541..47058
                     /gene="lysS"
                     /locus_tag="OO244_001137"
     CDS             45541..47058
                     /gene="lysS"
                     /locus_tag="OO244_001137"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5663045.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47101..47649)
                     /gene="idi"
                     /locus_tag="OO244_001138"
     CDS             complement(47101..47649)
                     /gene="idi"
                     /locus_tag="OO244_001138"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5663046.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47704..47775
                     /locus_tag="OO244_001139"
     CDS             47704..47775
                     /locus_tag="OO244_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663047.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47772..47897)
                     /locus_tag="OO244_001140"
     CDS             complement(47772..47897)
                     /locus_tag="OO244_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663048.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47899..49347)
                     /gene="uacT"
                     /locus_tag="OO244_001141"
     CDS             complement(47899..49347)
                     /gene="uacT"
                     /locus_tag="OO244_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5663049.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11981 a        12412 c        13353 g        11861 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caagcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacgaaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgagtgaa gccgttactc gcccaaagcg gcccgcttga
     2401 cgatattgat gttgcgctgc gtttgattta tgcgctgggg aaaatggata aatggctgta
     2461 tgccgatatc acgcactttt cccagtactg gcattacctg aacgaacagg atgaaacgcc
     2521 cggatttgcc gatgatataa cctgggattt tatcagcaat gtcaacagta taactcgcaa
     2581 tgcgacgctc tacgatgcgt taaaagcgat gaagttcgcc gatttcgccg tctggtcaga
     2641 ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa
     2701 ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc tgaacgtgca agcgcagtac
     2761 catgatgaag agatagaacg cgtgcataaa ctgttgctgc gtatgctggc ggccttacag
     2821 acggtaaatc cgcagcggcg tacggtggtt tttctttgcg ctccgccggg gacgggtaaa
     2881 tctaccctaa ccaccttctg ggaatatctc gcgcaacaag acccggagct gcccgctatt
     2941 caggcgctcc cgatggatgg ttttcaccat tacaatagct ggctggacgc gcatcaattg
     3001 cgccccttca aaggcgcacc agagacattt gacgttgcga aactggcgga aaatctgcgc
     3061 caggtggtgg aaggggattg tacgtggccg cagtacgatc gacaaaagca tgatcctgtt
     3121 gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg gttgttgctg
     3181 gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt tctcgatctt tatcaacgcg
     3241 ccagctgctg cgttgcggga gcgtctggtg gggcgcaagc tggcgggagg ggtatcgctg
     3301 gcagatgcag aagcctttta tgaccgtacc gacgggccga atgttcgccg ggggctggaa
     3361 gagagtctgc ctgcaaattt gaccttaatg atgaccgcta cgggtgaata tcgtctggtg
     3421 gattaacgtt cctgattaca ccatcttcca gccgcaccac acgagagaaa tgacgccggg
     3481 taaatgcggc gtcatgactg attgctacca cgctcgtgcc tcgctggcgg cacttctcca
     3541 gccagctttc aaacacgctc aaccaatttt cgtcaaaatc ccgactcggc tcgtccagca
     3601 gtaatagtgg cggagagagt gcttccaggc aggcgacggc gaccatccgt cgctgcgcat
     3661 tatgcagatc caacgggtgc gagttggcag tgtctgctaa ctgacaacat tgcagggcag
     3721 catgggtacg ttgagtgatt tcatccgccg ggcatttttg cagtttcaaa ccaaatgcga
     3781 tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc ttcctgaaac agtacgccaa
     3841 cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc ctgttgttgc agcataaccg
     3901 taccggctgt gggcgtaaga agacctgcca ttactcgtaa cagcgtcgat ttccctgcgc
     3961 cattgtcacc ggtcagcgcc aaccattcac cttgtttgag ttgcagagaa atgtcgcaaa
     4021 gacaatccgt tgccgcgcca ggccagcgat aagatatctt atttaacgtt aacatagtgg
     4081 ctggagcgct ccgttttgca attgccacgc ttgctggcag tagttcagga aaggtgtgcg
     4141 atgacgttca aaaagaataa tcagcgaacc acgttcaaac gcccagtgtt gtaagcgctg
     4201 caacagcatt tcgctggcct gcggtgtcag gcggctaaag gcttcatcaa gaattaagag
     4261 ctttggctgc atcgcgatgg cgcaggcgat gatcacgcgc tgtgtctctc caccggagag
     4321 tgttgccggg tgacgatggc gcaacggctg gcactcggtc agagtcaggg cggcatcaat
     4381 ccgcgccata atctctgctt cagcaagaca cagattttct ggcccaaagg caacttcttc
     4441 ttccacgcta aaggtacagc cggagagctg caaatagggc gattgctgga cgagctggat
     4501 ggtggcggat tgttcattaa gcgggagatg cccaatgggc gtaccgagga gcgtccccgt
     4561 gcctgttatc tctcttggca gaaagtccgg ataccagccc gccatcagct gtgccagcgt
     4621 acttttgcca ctgccgttgt caccaaagat ggcgaccatc cccggtttga catagtgaaa
     4681 gtcataacag aacggaggat gcgttgagtg tgtcgggcaa taacggaact gttctaacgt
     4741 aaccatatcc acgctccgaa ttctgtcagc atcagcaaga tcatggtgta acgcgctacg
     4801 cgttgtaggg tgctatcggc tggtgcccac aacgttgttc gtcggttgtg gatccgaaat
     4861 gcccgcatat ccagcgccgc accacggaca gccagatcgt taagcgcatt gtgtgttagc
     4921 ggaataatta acgcgggcat agcacgtaaa cgctgatacc agccctcatc cagcggcacg
     4981 ccgcgggcac gctgcgcttc atgaataatc gctaattgcc gttttaactg ctcaacgacc
     5041 aataacggcc cggcaaacag ataagcgacg cctggcggca ggcgagaagc aaacagagcg
     5101 cgaataaaac attgcacggg gacgtactgc attcacaact gtgaggttga aacaatcgcc
     5161 agaatcagca accagagcgt aatagcatgc gaccaccgtt ctggcgagcg tggcgtgccg
     5221 cttagccatt ctggtagcca gccaccgtgt accagccaca gcccggctcc cagcgagaac
     5281 atcagccaga cgacatattt tgcccgccgc cgcgtggcct ttagcgcaat aaggcagaaa
     5341 aaggtcgccg cgctatagat cggcaaaatg gtctgcgcgg gcaggataag cgtggtgcag
     5401 gccgctaatg cccacagtgt taatgaggta aatggatgca tttagcgcac ggcagccatt
     5461 gccggaaaat ggcgcgtggt gcgaatcggc agctggcgca gtaaaagcca gacaatcacc
     5521 gctgtaagaa ttttatcgac caggttcgcg cctatcaccg taatcgccac cgactccacc
     5581 aggttttgcc ccatcgagtg catccaggca acgaacaaat cggctccgct accggtgaca
     5641 ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg ttaccgcaag cgtaataatc
     5701 acgccgctga caacgacttt tggcagggtg cgaaaccaac ccgctcgcgc cagccaaccg
     5761 gagaccaggc cgatgaccat cgcgacgggt gcaaatgcgg cggcgatcgg gtcggtcagc
     5821 aatccccaga gtaaattggt cagtaagccg gtcagcattc cgatcaccgg accaagcagt
     5881 accgcgctaa taagtgtgcc gatggagtcg agaaatatag gtaatttgac catgctggcc
     5941 agttgcccgc cgatcatatt gatggcgata gatataacga tcaacaccag tgcctgactg
     6001 gaaaagtggc tacgcgccat aagaaaacct ctttgaaagt agttatttaa cgggggaaat
     6061 atgcaactcg tcgtaaccag aaagcgcgca aggttggcgg ctaaacgcca gttcctcaag
     6121 gctaccgatc accagttcaa gcgtggtccg cacggtacag ccgggcatca tatgaccgcc
     6181 aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga tgctcaccac tctgttccag
     6241 cgtgccgttt agtgagatca cttcgaatgt accgttcagg agcgtggtgc cttcttgccc
     6301 ggcatagcgc aaggcgacgt cagtcaggct accggtacag cctgcaatct aggcggcatg
     6361 aagttgatgt tgctgtgcaa aagcgtgtag ctgggaaaaa acttcctgcc cggggaggag
     6421 gcgcagtgcg taaaagcgtg cggaggaaga atatgagtag ggcaatgtca tcattggagt
     6481 actccgaaac tcggacgtat tgcggataat atcgacgctg catcagtcat attctgatat
     6541 aagtcagggt ttgtgacggc acaacgaaaa gattttgcta cgcttattcg tgattctgca
     6601 aagtggaata aattccacag gggcggggct gaaaacgtga cgtaagtcag ataattgctt
     6661 tctttgactg gacaatcatt ccttttattc cacgtttcgc ttatcctagc tgaagcgttt
     6721 cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag gtaaggtttc
     6781 cccggacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa ccttgcagga
     6841 gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt aatgtggttc
     6901 gtgctttgta tgaatccgga cgccgggcgg aaattaccgt ggtggcaatc aacgaactgg
     6961 cggatgctgc gggcatggcg catttgttga aatatgacac cagccatggc cgttttgcat
     7021 gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc cgcgtattgc
     7081 atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta gtcctcgact
     7141 gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca tattgccgcc ggggccaaaa
     7201 aagtgctctt ttcacatcct ggcagtaacg atctcgacgc gaccgttgtt tacggcgtca
     7261 atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt accacgaatt
     7321 gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc ggcactgtga
     7381 ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat cctgacctgc
     7441 gtcgcacccg ggcagccagc cagtcgatca ttccggtcga tactaaactg gccgctggta
     7501 tcacacgatt ttttccgcaa tttaacgatc gctttgaagc gattgcggta cgtgtgccaa
     7561 ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta aaagccaatg
     7621 aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt tcatggtata gttgactata
     7681 cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc attgtcgatg
     7741 gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac gttggtctgg tgcgataacg
     7801 aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact gttgctttca
     7861 ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt caccatgtct
     7921 gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg tgcggatctg
     7981 aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc ttctctgccg
     8041 accatcgaac tggccctgaa acaaggcgca aaagtgatgg taacttccca cctgggtcgt
     8101 cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa ctacctgaaa
     8161 gacaaactgt ctaacccggt tcgtctggtt aaagattacc tcgacggcgt tgacgttgct
     8221 gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa gaaagacgac
     8281 gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga cgcattcggt
     8341 actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga cgttgcgtgc
     8401 gcaggcccgc tgctggcagc tgaactggac gcgctgggta aagcactgaa agaacctgct
     8461 cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac cgttctggac
     8521 tccctgtcta aaatcgctga ccagctgatt gttggtggtg gtatcgctaa cacctttatc
     8581 gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgacctggt tgacgaagct
     8641 aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgcgt agcaaccgag
     8701 ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc tgacgagcag
     8761 atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa gaatgcgaaa
     8821 accattctgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg caaaggtact
     8881 gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct ccatcgctgg cggcggcgac
     8941 actctggcag caatcgacct gttcggcatt gctgacaaaa tctcctacat ctccactggc
     9001 ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc gatgctcgaa
     9061 gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcacttgtt tcagcgcgct
     9121 tttagagcac gcattttttc atactctaaa taattcgagt tgcaggaagg cgacaagaga
     9181 gtgaatcccc aggagcttac ataagtaagt gactggggtg agcgaacgca gacgcagcac
     9241 atgcaacttg aagtattatg agtataaggc ccgacgatac aggacaagag acatgtctaa
     9301 gatttttgat ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga aagttttcca
     9361 ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta ctgactccat
     9421 caacgccgta ctggaaactg ctgctaaagt taaagcgccg gttatcgttc agttctccaa
     9481 cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct gacgttccgc agggtgctgc
     9541 tatcctgggc gcgatctctg gtgcgcatca cgttcaccag atggctgaac attatggtgt
     9601 tccggttatc ctgcacactg accactgcgc gaagaaactg ctgccgtgga tcgacggtct
     9661 gctggacgcg ggtgaaaaac acttcgcagc taccggtaag ccgctgttct cttctcacat
     9721 gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa atctgctcta aatacctgga
     9781 gcgtatgtcc aaaatcggca tgactctgga aatcgaactg ggttgcaccg gtggtgaaga
     9841 agacggcgtg gacaacagcc acatggacgc ttctgcactg tacacccagc cggaagacgt
     9901 tgattacgca tacaccgagc tgagcaaaat cagcccgcgt ttcaccatcg ctgcgtcctt
     9961 cggtaacgta cacggtgttt acaagccggg taacgtggtt ctgactccga ccatcctgcg
    10021 tgattctcag gaatatgttt ctaagaaaca caacctgccg cacaacagcc tgaacttcgt
    10081 attccacggt ggttccggtt ctactgctca ggaaatcaaa gactccgtaa gctacggcgt
    10141 agtgaaaatg aacatcgata ccgataccca atgggcaacc tgggaaggcg ttctgaacta
    10201 ctacaaagcg aacgaagctt atctgcaggg tcagctgggt aacccgaaag gcgaagatca
    10261 gccgaacaag aaatactacg atccgcgcgt atggctgcgt gccggtcaga cttcgatgat
    10321 cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc gacgttctgt aagtcattcc
    10381 tttctgctta tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc aaggtgattc
    10441 agatgagaaa tctgtgatct atttggcaaa attatgcttt attgtttacc cttgtcagac
    10501 tgcccgtcat aaggcggcgg agtgtatttc tccattttga gtcagttgaa aaggaatatt
    10561 gaatggaaga tttgaatgtt gtcgatagca taaacggcgc gggaagctgg ctggtagcta
    10621 accaggcgct gctgctaagt tatgcagtaa acatcgtggc ggcactcgcg atcatcatcg
    10681 ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa tcgcctgatg atctcccgta
    10741 aaatcgatgc cactgttgct gattttcttt ctgcattagt ccgttacggt attatcgcct
    10801 ttacgctaat cgctgccctg ggacgcgtgg gtgtacaaac agcgtcagtc attgctgtac
    10861 tcggtgccgc aggcttagct gttggtctgg ctttgcaggg gtcactttct aacctggccg
    10921 ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg agaatatgtt gacctcggcg
    10981 gcgtagccgg tactgtgctg agtgtgcaga ttttctccac caccatgcgt actgcagacg
    11041 gtaaaattat cgttattccg aacggtaaaa ttattgccgg aaatattatt aacttctccc
    11101 gcgagccagt tcgccgtaac gaatttatta ttggcgtggc gtatgattcc gatatcgatc
    11161 aggttaagca gatcctgacc aatattatcc agtctgaaga tcgcattttg aaagatcgcg
    11221 aaatgactgt gcgcctgaac gaacttggtg catcgtcgat taatttcgtg gtccgcgtct
    11281 ggagcaacag cggcgatctg caaaacgtgt actgggatgt gctggagcgt attaaacgtg
    11341 aatttgatgc cgccggtatc agcttcccgt acccgcaaat ggatgtgaac tttaagcggg
    11401 tgaaagaaga caaagctgcg taatcaacgc tgaggccaga taatactctc tggcctctct
    11461 cttattagtt attctgattg ccaattaata ttatcaattt ccgctaataa caatcccgcg
    11521 atatagtctc tgcatcagat acttaattcg gaatatccaa cgtgttttct tattactttc
    11581 aaggtcttgc tcttggggca gctatgattc tgccgctcgg cccacaaaat gcttttgtga
    11641 tgaatcaggg cattcgtcgt cagtaccaca ttatgattgc cttactttgc gctatcagcg
    11701 atttggtcct gatttgcgcg gggatttttg gtggtagcgc gttattgatg cagtcgccgt
    11761 ggttgctagc gctggtcacc tggggcggcg tagtcttctt gctctggtat ggttttggcg
    11821 cttttaaaac agcgatgagt agtaatattg agttagctag tgccgaagtc ctgaagcaag
    11881 gcagatggaa aattatcgcc accatgttgg cagtgacctg gctgaatccg catgtttacc
    11941 tggatacttt tgttgtactg ggcagccttg gcgggcagct tgacgtcgaa ccaaaacgtt
    12001 ggtttgcgct cgggacaatt agcgcctctt tcctgtggtt ctttggtctg gctattctcg
    12061 cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca gcgcattatc aatctggtag
    12121 tgggatgtgt catgtggttt atagccttgc agctggcgag agacggtatt gctcatgcac
    12181 aagccttgtt cagttaggcg ctatctgatg gaaaaataaa acagaggcgc taagcttgcc
    12241 tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa
    12301 agttatcgcc ctggcggcat taatgggtat tagcgggatg gcagcgcagg ctaacgaatt
    12361 gccggatgga ccgcatattg tcacctccgg tacggcaagt gtggatgcgg tgccagacat
    12421 tgccactctt gcgattgaag ttaacgtggc cgcgaaggat gccgctacag ccaagaaaca
    12481 ggcagatgag cgcgtcgcac aatacatttc cttccttgaa ctcaatcaga tcgcgaaaaa
    12541 agatatcagc tcagcgaatt tacgcaccca gccagattat gactatcagg atggtaaaag
    12601 tatcctgaaa ggctaccgag ctgtgagaac ggtggaagtc acgctccgtc agttagacaa
    12661 actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt aacgaaattc gttctgtgtc
    12721 gctgggcgtg gcgcagccgg atgcctataa agacaaagcg cgtaaggcag cgattgataa
    12781 cgcgattcat caggcgcagg aactggcgaa cggctttcat cgtaaactgg ggccggtata
    12841 tagcgtgcgc taccatgttt ccaactatca gcccagccca atggtgcgga tgatgaaagc
    12901 cgatgccgcg ccggtgtctg cccaggaaac ttacgagcag gccgctattc agtttgatga
    12961 tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa caacccgcta aaacacctgc
    13021 agcacaataa tacttgtagc ccgacgtatt cgtcgggcta atgcctttac ccgatattgc
    13081 ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt
    13141 tcatcaagga taggctgact gaaacggtta agcggacaag gatgcgggga ggggggatgg
    13201 acatttttat ttcgaaaaag atgcgcaatt tcattttatt agcgcaaacc aataatattg
    13261 cccgggcggc agaaaaaatc catatgacgg cttcgccatt tggtaaaagc attgccgcgc
    13321 tggaagaaca aattggctat acgctattta cccgcaaaga taataatatc agcctcaata
    13381 aagccggtca ggaactgtat caaaaactgt tcccggttta tcaacgactt tctgcgattg
    13441 ataatgaaat ccataactca gggcgtcgtt cacgagaaat tgtgattggc atagataata
    13501 cttatccgac aattattttc gatcagttga ttagtctcgg agataagtat gaaggcgtga
    13561 ccgcgcagcc agttgagttc agcgaaaacg gcgttatcga taatcttttt gaccgtcagt
    13621 tagattttat tatctcccca cagcatgtat ctgcacgagt tcaggaactg gaaaacctga
    13681 ctatcagtga attaccaccg ctgcgattgg gctttctcgt ttctcgccgt tatgaagagc
    13741 gtcaagagca agagctgctt caggagctgc cctggctaca aatgcgcttt caaaaccggg
    13801 ctaacttcga agcaatgatt gatgcgaata tgcgaccttg tgggatcaat cccaccatta
    13861 tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt ggagcgcgga cattttctga
    13921 cggtgatccc gcattttgcc tggcgactgg tgaatccggc aacgctgaaa tattttgatg
    13981 cgccgcacag gccgatgtat atgcaggaat atctttattc aatcagaaat catcgttata
    14041 ccgccacgat cttcagcata ttgctgaaga tcgtgacggg acaaaccatt aacccagcat
    14101 cgagccggtt gcaattaaat tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat
    14161 atgtccgccg ggcgcatttt ccagatagcg atgcagataa tcccgataca taggatgtgc
    14221 acaattatca atgatagtgc gggcgcgttg aagcggggaa agaccgcgca gatcggcgat
    14281 cccttgttcg gtgatgatca ctttgacgct gtgttcgctg tgatcaacat ggctgcacat
    14341 tggcacgacg gttgagatct tgccttcttt agcaatcgac ggagccataa agatcgacag
    14401 atacgcgttg cgttcaaaat caccgctgcc gccgatgccg ttcatcagat cgaccccggc
    14461 tacgtgcgtt gaatttgcat gcccgtaaat atcaaactcc aggccgacgt tcagagcgat
    14521 gacgcccaga cgacggatga tttccgggtt attggaaatc tcctgcggac gcaacacaat
    14581 gcggctggca aagtaatcca tattgtcgta aatcttgcgc agggaatcgg ccgagattgt
    14641 caggctggag gcgctggccc cgctgatttt gccggtttcc agtaaatgca ccaccgattc
    14701 ctgtagcact tccgaataca tcatgaacgg aggaatttcc gggttttccc ccagacgtgc
    14761 cattaccgca ttattgatat tgcccacgcc actttgcagc ggcagaaatt ccggcggaat
    14821 acgcccatgc gccatttcct gcaataagaa cgtgaccacg ttatcggcaa tctgctggca
    14881 catgggattt tgcttatcca gcatattacc ggcgtcgggc aagttggttt ccacgacggc
    14941 gacaatcttt ttcggatcga tttgcacata gcgggtaccg acgcgatcca ttgcatggaa
    15001 gatcgacacg ctattgcgcc gcggtggcgc gccaggaatc acaatatctg ccagttctgc
    15061 aacgcgcgga tcgtgatagt gattaagttc aatgatcact ttcttcgctc gcagcagcta
    15121 ggtcggcgca ttaccgatcc cgctggttaa ccagactcga ccatccggtg ccagtgccga
    15181 tgcttcaatg acggcaacat caatgtcgcc gaagaaaccg taattgacca tttgcgccac
    15241 ttcgctcaaa tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt tacgtaaact
    15301 ggacgatgtt tgatatggcg cacgccagga aacagcatcg gcgtcagaaa gtacatcgtc
    15361 agcggcggcg ctgattgacg cccccgtcag caggcgaatt tgatacggct ttttggcctc
    15421 atgctgttcg ttagctctgc gggcaatcgc ggcgggtagg gctttcggcg aacccgctgg
    15481 ggtaaagccg ctaaatgcca ccatgtcgtt atgctggata atttctgccg cttcatcggc
    15541 ggtcatcctt gtccactgag tttccatttt tactccttcg ttcgcaggga ttaatgacca
    15601 acgaaattag gtttacgttt ttcgaggaaa gcgttcatcc cttcctggta atcttcgctg
    15661 tcataaaccg cgcggcgcat cccctgaata cgttcaaatt catcggagtt catggtgtgt
    15721 gcttcgccca gtacacgcag ctcttctttg ataacggcaa tggctaacgg cgctttctca
    15781 gagatgtggt gcgccatttg taaggtgaaa tcttccagtt cttccacttc cacaacatgg
    15841 ttgaggatgc cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc
    15901 tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac
    15961 gggacgccga ggtttacagg cgtcattgag aaggttgagg tactggcggc gatgatcaga
    16021 tcggaactca tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaatg
    16081 atcggtttcg ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag
    16141 agcggatcgc gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg
    16201 gatccacttg gcgcccgcaa aatgatacag cgaatttccg gccggttgag atcgcttaac
    16261 gcctgcataa gatcatcaat aaagacttta cttaaggcat taagttttcg tccatagtta
    16321 aactcaatga ccgccacttt gttgatagtg gcaacgttca catactgata agacataaaa
    16381 attcctttaa tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg
    16441 cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg cggtaatagc gatcgaaatc
    16501 ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac
    16561 cgattgttgt tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtc
    16621 gatgatggcg tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagacc
    16681 ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg
    16741 ccgggcaatg gcgacattgg tatggttatc gccatcgtct ttgttgataa cgagcagatc
    16801 agccacttcc atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg
    16861 caacgagata aaacagtcca ccatgcgggc gacttccgtt tccgactgcg cgatgccaac
    16921 cgtttcgaca atcactacgt cataacccgc cgcttcgcat aacagcatta attcccgtgc
    16981 tcgctgactg gcaccgccca gatgaccgga ggaggatacc gggcgaataa acgccgcctc
    17041 ggcacgcgcc aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg
    17101 gctgctggga tcgaccgcaa taacagcaac ctttaatccc tcacgaatca acaacatgcc
    17161 aaaggcttca agaaaggtac ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag
    17221 ggcgttacca cagaacggca taatagcgtc aagcagctgt gtacttagcg cctgatgacg
    17281 tgggtgacgg ctttccacca gcgtcatggc ttgggcgagc gtggcacgct caccctgacg
    17341 taagcggcga atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat
    17401 tcagtacgtc gtgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc
    17461 cgcgctcttg caggaaggcg taatcctgcg gcggaatgac gccacccgcg accacgcaga
    17521 tatcttcgcg tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac
    17581 cggcagccag tgaggatgcg cccactacgt gaacgtcgtt ttctacggct aggcgggcga
    17641 tctcttcagg tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg
    17701 cgctggcgat cactttcgcg ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa
    17761 tgcgcgggcg acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga
    17821 actcggaggc cgatttctca gactgatgat agctttgcgc aatcacgccg gtaacacact
    17881 ggcttggcac cagataacgg tcgaaagcga cttccagcgc atcggaaatt tcacccgggg
    17941 tggcgcgaac gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg
    18001 cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc
    18061 gttccagcga agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg
    18121 tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt
    18181 cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt
    18241 cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt
    18301 tgacgatttg atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgt
    18361 ggcagagttc tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga
    18421 aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc
    18481 ccagcgtcgc agccagtgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt
    18541 cagtcaggct ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcggatcct
    18601 gtgcgccaaa tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga
    18661 cgttcataaa cagatccatg ccgataccga agaataacga caggcgagga gcgaagtcat
    18721 caattttcag tccggcagag attgctgctt tgatgtactc aatcccatcg gcgagcgtaa
    18781 atgctacctg ctgcacgcag ttggcacccg cttcccccat gtggtaaccg ctgatactga
    18841 tggtattaaa tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca
    18901 ttgacggttt tggtgggtaa atataggtgt tgcggcagag gtactctttg agaatatcgt
    18961 tttgaatggt gccggtcagt ttatcaggtg taacaccttg ctcttctgcg gcgacgatat
    19021 aaaacgccag tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca
    19081 gcgggatctg gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt
    19141 tgccgacgtc acccgccacg cgtgggttat cggagtcgta gccacggtgg gtggcaaggt
    19201 caaacgcaac ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg
    19261 actcttttgc tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca
    19321 tagtggcacg cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca
    19381 gattatcgag atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct
    19441 gaaccagcga gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc
    19501 actcctgctc gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg
    19561 cttacggtga gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt
    19621 tttttgccgc ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg
    19681 aggacaacgc ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac
    19741 tttgtgacca taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc
    19801 aaagcggtgc cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc
    19861 accgctggcc agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg
    19921 gcgagcaagt tgtacgaacg cttctgaaga attaacgata tggcagggta cgctgccagg
    19981 aggaagatcg aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc
    20041 gaccactggc gctttcaata atgccgaacg cgttacgccg ttaggaaaat atttttcggc
    20101 aaacggtttt gagctgacga acagatagtc gagcgcaccg agtttatcga caagacaact
    20161 cggcagcgcc tgatgttgaa tactcaccgc gccgaccact tcgccgcggc gcagacgttc
    20221 ctgagtacgg gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac
    20281 aggagccagt gccggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag
    20341 cagcagcgga gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac
    20401 ctggcgcagc agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg
    20461 caccaacagc ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga
    20521 ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc
    20581 cagtgcctgt aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat
    20641 gctttttcct gcactatgac acaattttgt gtcaggttgc atataccgaa gccgtcggcg
    20701 ggtaacattt gtatcaatgg ttaaacgcgc agcttaaaag atcagggatt tgcgctattg
    20761 catttcgctt agcgacttcg cgccctggtt atgttgaatg gcgtggcgtt attgcctctg
    20821 ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg gcgaaagggg
    20881 atgcccgcca ttgcgcgata ttttatggat gagttaacca cccggcttgc caacggggtc
    20941 tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat
    21001 gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg
    21061 taccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggcac
    21121 gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa
    21181 aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga
    21241 tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac
    21301 ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg acgcctccaa
    21361 gcaggttgat attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag
    21421 tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtggt
    21481 gaccgataat ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg acccgatagc
    21541 gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg
    21601 tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc
    21661 tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca
    21721 cgcttttacc aggcaattgt cgattgctct aaataaatcc tctaaaccag catattcatc
    21781 caagaattac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc
    21841 aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga
    21901 caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt ttctgctggt
    21961 agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca ccaacatcga
    22021 atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg atgcccactt
    22081 catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg cagaaaaact
    22141 ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg cggcggcaaa
    22201 gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg cggagctggt
    22261 gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg
    22321 tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat
    22381 catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc tgggaatgta
    22441 tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc aggtacagca
    22501 tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag agaatccgtc
    22561 caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct cgctgctgat
    22621 taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa
    22681 acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca atagcgatcc
    22741 atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca ttggcggttc
    22801 gactcaggaa gcgcaggaga atatcggctt ggaagttgcg ggtaaattga tcaagtattc
    22861 tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg
    22921 tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa
    22981 aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat
    23041 gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc
    23101 aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc ccttctctga
    23161 aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac accgttgccg
    23221 cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca gatcaatgcg
    23281 ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta acggtaaagc
    23341 cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca tcatactgat
    23401 tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcaaggg aggagtcatc
    23461 ctcccttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg
    23521 cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca cgtttactgc
    23581 cgtaatagat cctttttccc aaaaattatc tctgttttgc atggggtttt tcgagcacgc
    23641 tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt atttaaagag agcttacgat
    23701 agctgggcag catggggctg taacggtaaa gccctcaccg aagcgagggc ttgaaggaga
    23761 agggttatga tgcgacttgc catcatactg attgtactgt tactcataag tttcccagct
    23821 tattaacagt cagtcacagg ggaggagcaa tcctccctta cccttactca ctaaattagg
    23881 ttaaagaatc aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga
    23941 ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt
    24001 ccaccaactg acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt
    24061 gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg
    24121 cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg tagcacgtca cgcacatcca
    24181 gttttggctc atggatcttc aacctgttca tcaccagttc gctttgcgga tggtaattca
    24241 ggaacagcaa attaccggca ctaaagggat gcaaaactgg aagatatacg cgcttaccgg
    24301 cgcgccagag ttgttctatt agcggctggg tgtcgagttc accatcaaaa gagaggaata
    24361 cagcgaccgt atgcgccatc accaccgggg gataagtcat catccgggta gcggcttgtt
    24421 gacccatttc cagctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc
    24481 ggatttcttg tcgggataat gtcagtggga gctctggtag ttgcgtcatg gctggtgcca
    24541 gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa
    24601 ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgaa ccgagcatgc tcaccaaccg
    24661 cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac
    24721 atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc
    24781 aaagttttgg ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt
    24841 ctgctgcagc atccgaatac gctgttccat acttgccgcg tagtcacgag tctttgcttt
    24901 ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt
    24961 tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg
    25021 attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg
    25081 gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc
    25141 tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc
    25201 ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac
    25261 gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc
    25321 tgaaccaaca agggacgggt ctgacccctg ccgagatgca tggtttaatc agtgggatga
    25381 tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg acgaacgaag
    25441 gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct gccaccagcg
    25501 atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca
    25561 gcgttttcga tcgggctgat gcactggcag gttgggtcaa tcacttcctg cttggtcttg
    25621 gcgttacgca accgaagctg gacaaagtga ccggcgaaac cggtgaagcc atcgacgatc
    25681 tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag cttgaaatgt
    25741 cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta
    25801 ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa
    25861 cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc
    25921 ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca
    25981 cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc
    26041 tttaacgaac cggaagcggt gctggtgctg attaaaagcg atgacactca taaccacagc
    26101 gttctgttta accgcgttcg cgacctgacg acggagatct ggtttggccg tcgcttaggc
    26161 caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaatcaat
    26221 cagcaacttt atcaactact taacggcctg aatgtggttt accatgccca gggcgaatat
    26281 gcatatgctg atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa
    26341 aatctcaccg caccggcaac gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg
    26401 ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg gagaaatcac cgccatggca
    26461 catacacggg cgatggaaaa atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa
    26521 attcaccacg aatttaaccg ccacggtgcg cgctatcctt cctataacac cattgtaggc
    26581 agcggtgaaa acggctgcat tctgcactac accgaaaacg agagtgaact gcgcgacggt
    26641 gacctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgcgggcga tattacccgc
    26701 accttcccgg tcaacggcaa attcacccag gcccagcgtg aaatctacga cattgtgctg
    26761 gagtctctcg aaaccagcct gcgcctgtat cgtccgggaa cttccattct ggaagtcact
    26821 ggtgaagtgg tgcgcatcat ggttagcggc ctggtaaaac tcggcatcct gaaaggtaat
    26881 gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac
    26941 tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg
    27001 gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg
    27061 ccagaacaat atcgcggtat cggcattcgt attgaagacg acattgtgat taccgaaacc
    27121 ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg
    27181 gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc
    27241 tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga
    27301 ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg ctggcggcgg
    27361 gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggctgat tgcgcaaccg
    27421 ccatcactac cgtgcatgtc agcgatcgtg gtcatgctgg attcgtcacc ctcgccgcag
    27481 aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc gggcaacggc
    27541 tgtttgcatt gctgcgaaaa gcacctggcg taatgctgca ttgccccgat cgcgtagcta
    27601 acgttgcccg tactcagagt catgttgaag tgacgctgga gagcggcgag atgctgacgg
    27661 gccgcgtgct gatagccgct gatggcaccc attcagcgtt agccaccgcg tgcggcgttg
    27721 attggcagca ggagccttac gaacaactgg cagtgattgc caacgttgct acttctgttg
    27781 cgcacgaagg ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg atgttgccga
    27841 tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc
    27901 tgtcgtggag cgacgagaag ttttgccgtg aactccagtc tgcctttggc tggcgactgg
    27961 ggcaaattac ccacgccggt aaacgcagtg cttatccgct ggcattaacc cgcgccgcca
    28021 gaccgattac ccatcgcact gtgctggtgg gcaatgcggc gcaaactctg cacccgattg
    28081 ccgggcaagg gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa accctgactc
    28141 aggcgcatga gcgcggagaa gacatcggtg attacggcat attgtgccgc tatcagcagc
    28201 gtcgacagag cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta catctttttg
    28261 ccaaccgttg ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg atggaattat
    28321 tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc
    28381 ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg cggcatggtg
    28441 gggctggcgg ttgcctgcgg cttacagggg agcggcttac gcgttgccgt actggagcag
    28501 cgcgtaccgg aacctctggc ggtagatgca ctaccacaac tgcgcgtttc ggctatcaat
    28561 gccgccagcg aaaaattact cacccgtctt ggcgtctggc aggacattct cgctcgtagg
    28621 gccagttgtt atcacggcat ggaagtgtgg gacaaagaca gttttggtca catctcgttt
    28681 gacgatcaaa gcatgggcta tagccatctt gggcacatcg ttgaaaactc agtgattcac
    28741 tacgcgctgt ggaacaaagc gcagcagtcg tcagatatta ctctgttggc ccccacagaa
    28801 ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgctgaaaga tggcagcatg
    28861 ttaacggcgc gtctggtgat tggcgcggac ggtgctaatt cctggttgcg caacaaagca
    28921 gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac cattcgcacg
    28981 gaagaaccgc atgatgcggt ggcgcggcag gttttccatg gcgaaggcat tctggccttt
    29041 ttaccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc agaggaagcg
    29101 cagcggatgc agcaggcaag tgaagacgaa tttaatcgcg cgttaaatat cgcttttgat
    29161 aatcgccttg gcttatgcaa ggttgagagc gcgcgtcagg tgttcccgct gacggggcgt
    29221 tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc gcataccatt
    29281 cacccactgg cggggcaggg cgttaatctc ggatttatgg acgccgcaga gctgattgct
    29341 gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta cctgcgccgc
    29401 tatgaacgta gccgcaagca cagcgcggcg ctgatgctgg ctggtatgca gggttttcgc
    29461 gatctctttt ccggcgctaa tccggcgaaa aaactgctgc gtgatattgg tttgaaactg
    29521 gccgacacgc ttcctggcgt taagccgcaa cttatccgcc aggcaatggg attaaacgat
    29581 ttgcctgaat ggctgcgtta aaaatttctc ctctgttgtt tatttgatac ccatcacact
    29641 ttcatctccc ggttttttcg ccgggagatt ttcatcattt gaaataaact aatttcacct
    29701 ccgttttcgc attatatttt ctaataccat gattttttga tttagtgttt tttgacattt
    29761 ttttagctct taatattgtc ttattcaaat tgactttctc atcacatcat ctttgtatag
    29821 aaattggtgt attttttggt tttttattct gtcgcgattt ttgcattttt taaccataag
    29881 ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc
    29941 aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt tgtggtgata
    30001 atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag
    30061 actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg
    30121 atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc
    30181 ggaatgtttg atgtgtcgca tatgaccatc gtcgatctcc gcggcagccg cacccgggag
    30241 tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt
    30301 tactcgggga tgttgaatgc ctctggcggt gtgatagatg atctcatcgt ctactacttt
    30361 actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg
    30421 attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg
    30481 attgccgtgc aagggccgaa tgctcaggca aaagctgcca cactgtttaa tgatgctcag
    30541 cgtcaggcgg tgaaagggat gaaaccgttc tttggcgtgc aggtgggcga tctgtttatt
    30601 gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg
    30661 gccgatttct ggcgtgcgct ggtggaagcg ggcgttaagc cctgcggtct gggggcgcgt
    30721 gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaactatt
    30781 tctcctttag ccgccaacat gggctggact atcgcctggg aaccgacaga tcgtgacttt
    30841 atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact ggttggtctg
    30901 gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac cgatgcgcag
    30961 ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct gggttacagc
    31021 attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac
    31081 cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg
    31141 tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca
    31201 aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt attaccgaac
    31261 atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg
    31321 ttagcgcggg cgatgactgc gcggttgccg aatcggtaaa agcggcgtca gacatttatg
    31381 cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgattcc ccggaactgg
    31441 tgaacagcga accgtatgca ggcggctgga tctttaaaat caaagccagc gatgaaagcg
    31501 aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagat gagtaacggc
    31561 tttattcctc ttctgcggga gaggatcagg gtgaggaaaa tttatgcctc accctcactc
    31621 tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc gctcatgaca
    31681 cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat cggaccggac
    31741 gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc
    31801 ggccagattg tgccgaaaga tattcagctt gcgacaccac cgcaggttgg cgcaccggcg
    31861 accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa acgcttcacg
    31921 tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct gcgtaacatg
    31981 ctggaaaatc cgggctggta taccgcgtac actccgtatc agcctgaagt ctcccagggc
    32041 cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg actggatatg
    32101 gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc gatggcgaaa
    32161 cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccaatgt gcatccgcaa
    32221 acgctggatg tggtccgtac tcgtgctgaa acctttggtt ttgaagtgat tgtcgatgac
    32281 gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca ggtaggcact
    32341 accggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg caaaattgtg
    32401 gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg taaacagggc
    32461 gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca
    32521 cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg ccgtattatc
    32581 ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca gactcgcgag
    32641 caacatattc gtcgtgagaa agcgaactcc aacatttgta cttcccaggt actgctggca
    32701 aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg tatcgctaac
    32761 cgcattcacc gtctgaccga tattctggcg gcgggcctgc aacaaaaagg tctgaaactg
    32821 cgccatgcgc actatttcga caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg
    32881 acgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc cgtggggatc
    32941 acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc ttttcagtgt gctgctgggc
    33001 gataaccacg ggctggagat cgacacgctg gacaaagacg tggctcacga cagccgctct
    33061 atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt taatcgctat
    33121 cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga tctggcgctg
    33181 aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc cgccgagatg
    33241 atcccaatca cttggacgga atttgccgaa ctgcacccgt tttgcccgcc ggaacaggct
    33301 gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact gaccggttac
    33361 gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg cctgctggcg
    33421 attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct gatcccggct
    33481 tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt ggtggttgtg
    33541 gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc ggaacaggcg
    33601 ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt gtatgaagaa
    33661 acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta ccttgatggc
    33721 gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc ggacgtttcg
    33781 cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc gggtatggga
    33841 ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc
    33901 gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag cgcctctatc
    33961 ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa aaaagcaagc
    34021 caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc cttcccggtg
    34081 ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg
    34141 aaagaagaaa ccggcatcag cgagctggac attgccaagc gcctgatcga ctacggtttc
    34201 cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc gactgaatct
    34261 gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg cgcagaaatt
    34321 gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa cgcgccgcac
    34381 attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt tgcggtattc
    34441 ccggcaggcg tggcagacaa atactggccg acggtgaaac gtctggatga tgtttacggc
    34501 gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt cactgattcg
    34561 agtattttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac aaaagtgctc
    34621 agacagtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg ccggcactgg
    34681 cgcgaatatt taccctcacc ccgaccctct ccctgaaagg gcgaggggga aaagcgtgcc
    34741 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    34801 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    34861 caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat cagcaaaacc
    34921 tccacgcggc gcaggaagtg atgaacttaa taatgcaagc cggtggcaaa gcattcgtgc
    34981 tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca atcgatcagc
    35041 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    35101 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    35161 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    35221 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa tatgttgatt
    35281 acgcggcatc gaaaggggcg attgattcgt taaccaccgg actatcgctg gaagtcgccg
    35341 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    35401 gcggcggcga gcctggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg
    35461 gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg
    35521 tcacgggaag ttttatcgat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg
    35581 atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa
    35641 tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagtaccgt
    35701 cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg gtagtgaatg
    35761 acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca tcgtaagtca
    35821 ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga
    35881 tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg
    35941 gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct
    36001 gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca aaaccagaga
    36061 tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt catgtaatag ctgaaaccaa
    36121 gataatcgca ggtgccttcg cgcagtacat cgagatcgcc gtcttccatt ttgatattaa
    36181 atccgcgacg ttcccactcg ttcaatacat aggacgggta atagccgcat agctgcacat
    36241 cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac
    36301 atgagtaggg atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa
    36361 tgcgacgcgc ggctttcact gccagggcgc tggcgacaaa ctggtgatgc agcacctggt
    36421 acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag cagtaaccaa
    36481 acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc cagtatttga
    36541 ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa tcaacgaatt
    36601 tacggttggt ccagctaccg tattgctgca ccagatgcag cggcatttca aagtgggaga
    36661 gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag tcgtaaaact
    36721 tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga
    36781 tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt
    36841 gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag acttctttgg
    36901 tgatttcgcg cggcacgccg tgtgcgccac aggtcagaac gtcacaaatg ctcggtcctt
    36961 tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca ccccataaga
    37021 aatctttcgg taaggtgagt tttttcacta tcataggctc cagatcagtc aattcttaca
    37081 ggaaatagta ccatgcagcc aaacgacatc acttttttca acgtttccag gatgacattc
    37141 tggctgggcg taaaaccatc accatccgcg acgagtctga atcgcacttc aaaacgggtg
    37201 atgtgcttcg tgtcggacgt tttgaagatg acggttattt ttgcacgatt gaagtcaccg
    37261 caacctcaac cgtaacgctg gatacactga cggaaaaaca tgcagagcag gaaaatatga
    37321 ccctgcctga actgaaaaag gtcattgctg acatctatcc cggtcagaca caattttata
    37381 tgattgaatt taaatgtctt taaattgaac tcacgcctgt tagttgaaat ttcgcattta
    37441 atttcatgat ttttaggaga ttagtctcaa atcagaattt attttagcta acaggtgttc
    37501 actggaacta ttctcagtta cgctggagag gtatacatgt gcgtgtacgt ttctctggag
    37561 taagttatgg ttcagaagcc cctcattaag cagggatatt cgctggcaga ggaaattgcc
    37621 aacagcgtca gtcacggcat tgggctggtg tttggtatcg ttgggctggt gttgctactg
    37681 gttcaggcgg tggatctcaa tgccagcgcc acagcgataa ccagttacag cctctatggc
    37741 ggcagtatga tcctgctgtt cctcgcttcg acgctctatc acgccattcc tcatcaacgg
    37801 gcaaaaatgt ggctgaagaa atttgaccat tgcgctattt acctgttgat tgccggaacc
    37861 tacacgccgt ttttgctggt ggggctggat tctccgttag cgcgcgggtt gatgattgtt
    37921 atctggagcc tagcattgct ggggattctg ttcaaactga ccatcgcgca ccgattcaaa
    37981 attttatctc tggtgactta tctggcgatg ggctggctgt cgctggtggt aatttatgaa
    38041 atggcagtta agctcgcggc gggcagcgtt accttactgg cggtaggcgg cgtggtttat
    38101 tcgctcgggg tgattttcta cgtctgcaaa cgcattccat acaaccatgc catctggcac
    38161 ggcttcgtgc tcggcggtag tgtgtgccac tttctggcga tctatttgta tattgggcag
    38221 gcgtaatggg tatgcctttg taggcctgat aagacgcgtc agcgtcgcat caggcatcgg
    38281 agcgcaaccg ccggatgcgg cgtgaacgcc ttatccgatc tacgggcctc gtccctgttg
    38341 tgataaaggc atcaaatccc agacatgata agacgcgtca gcgtcgcatc aggcgataaa
    38401 tgcctgatgc gatacagaga tttactcttc gagcgaatac ggcagcggct cgatatgcag
    38461 tgtattcgca tcgtcgcgta cgcggaagat gctgtccggt tccatatcgt tattcatgac
    38521 gacctgtacc acgacctgac catcttccag ttttaccgca gccagcaccg taccggtacg
    38581 acgccagttc tcgcccattt tcagctctaa gtcttcacca gcttccggca gtcggctggc
    38641 actacctgcc agcaaccaga gcgcgcgttt gttggcacca cggaattttg ctcgcgccac
    38701 catctcttgt ccggtataac agcctttctt aaagctgata ccgcccagcg cctggagatt
    38761 ggtcgcctgt gggataaact gcccgctgtt ggcggcatca atcaccggga aaccagcttc
    38821 aatgtttaat gccagccact gttggctatt gttcagttcc gcttcaccgc gcagtttatc
    38881 ggtcagcata ttagcagtag cttcatcggt tacgatcagg aaacgttctg ccgggtgttc
    38941 aaaccatagc aaagtggtcg cgccttcttt gactacctgt ttttctttcg aaggcagttc
    39001 gctaaagaga tttgccagcg cggcgcgcgc ctgaaaaccg gcaacaccaa gcagcacacg
    39061 ctcgtcgtct ggcgcgatgg tcactttaga gaataccgca tattttttca gttcagtcag
    39121 ctgcggttca cgcacgctgc gccgttcaat ccatgcaaag ccatcgccgt cgcggaacag
    39181 acgtaaattg ctccacattt tacctttggc gtcgcaatgg gcggcgagca ggtgctgatc
    39241 ttctgccatc tggctgacat ctgctgtcac ctgaccctgc atatattttt cgctgtccgc
    39301 gccagtaatg gtggcaagcg cccagtcatc aagcgtcatc agcgtcagcg gtaaacgggc
    39361 agaagccgta ggctgacgtg gaggaaaagg tgtaaaagcc ataatagagt cctgagttgc
    39421 ttaacggcgt attaatgact aatggtaaaa gagctatgag gcaatgcaag tgctttcact
    39481 acgcgatctg tgccttctgc gaggcgactg ccaaaaaaat gaagcccttg aattgttgtg
    39541 aattaatttc gcgatcgcga tggtgtttac tgatattccc gtaaaagaag tcgaaaaaca
    39601 cgttacaata gccgggtact ggattttcgt gagacaggaa gaagatggac attaacaaca
    39661 aagcccgaat tcattgggca tgccgccgtg gtatgcgcga actcgatatt tcaatcatgc
    39721 cgtttttcga acatgagtac gacagcttaa gcgatgacga aaaacgcatc tttattcgtc
    39781 tgctggaatg tgacgatccg gacctgttta actggctgat gaatcacggt aaaccggccg
    39841 atgcagaact ggaaataatg gtccgactca tccagacacg gaaccgggaa cgtggtcctg
    39901 tggcaatctg atttgcgcgt ctcctggcgc gcacagtggc tttccttgct gattcatggg
    39961 ctggttgccg ctgttatttt actcatgccc tggccgctca gttacacccc gttatggatg
    40021 gtgttacttt cgctggtggt atttgattgc gttcgcagcc agcggtgtat taatgctcgc
    40081 cagggggaaa ttcgcttgtt gatggacggg cgtttgcgtt ggcaagggca ggagtggagc
    40141 atcgtcaaag caccgtggat gattaagagc ggcatgatgc tgcgtttacg ttctgatggc
    40201 ggtaaacggc aacatttatg gctggcagcc gacagcatgg acgaagccga atggcgggat
    40261 ttacggcgga ttttgttgca acaagagacg caaagataaa gatgacggca aagcatatgc
    40321 gctaagccgt cagtgagatc aggcgtaatg ctctgccatt tcgttgagga tttgctcgca
    40381 ccagctctga atacgctcgt cgctaaggtc atactggtta gtttcatcca gcgccagacc
    40441 cacgaacagt tgcccgtcag caatcaccgg tttcgggctg gtaaattcat atccttccgt
    40501 tggccagtag ccgacgaact tcacgccttt ggtcgagagt ttgtcatgca gcataccgag
    40561 cgcatcgagg aaccactcgc cgtatcccag ttgatcgcca agcccataca gcgcaataat
    40621 tttaccttca aggttcaggt cgtcgagctg atcccagacg gcttcccagt cttcctggat
    40681 ttcaccaaaa tcccaggtcg ggatacccag aatgagcaca tcgtactgct ccattaattt
    40741 cggggagtcg tccttgaggt tatgtaaggt caccagttct gggccgataa tatcgcggat
    40801 tttttctgcc gccatttcgg tgtaacaggt gctggaaccg taaaaaagac ccatattcat
    40861 aacgtaagct tctcgattct gctgtggctt ttgcagctag tgtatcagaa tcgctttagt
    40921 cacggcacaa taaggcataa tggatcaaat gagtgaccat aaggggcgca agtgaaacag
    40981 gagctggcac gcatcgagca gtttcttgat gctctgtggc tggaaaaaaa tctggctgaa
    41041 aatacgttga acgcttaccg tcgcgatctg tcaatgatgg tggagtggtt gcatcaccgc
    41101 gggttgacgc tggcgacggc gcaaagtgac gatttgcagg cattactggc agaacggctg
    41161 gagggcgggt ataaagccac cagctcagcg cgtttgctga gtgcagtgcg ccgattgttc
    41221 cagtatcttt atcgcgaaaa gtttcgtgaa gacgatccca gtgcgcatct cgcttcaccg
    41281 aaattgcccc agcgtttgcc aaaagattta agcgaagcgc aggtcgaacg tttattacag
    41341 gcgccattaa ttgatcagcc actggagcta cgcgataaag ccatgcttga agtgttgtat
    41401 gctaccgggc tgcgtgtctc tgaactggtc gggctgacaa tgagtgatat cagcctgcgt
    41461 cagggcgtag tgcgggtcat tggtaaaggc aacaaagagc gcctggtgcc gttaggggaa
    41521 gaggcggttt actggctgga aacctatctg gaacatgggc gtccgtggct gctgaatggt
    41581 gtgtcaattg acgtgttgtt tcccagccag cgtgcgcagc agatgacgcg acagaccttc
    41641 tggcaccgca ttaaacatta tgccgtgctg gcgggtatcg acagcgaaaa gctgtcaccg
    41701 catgtgttgc gtcacgcttt tgccactcat ttattaaatc atggtgcgga tttacgcgtg
    41761 gtgcagatgc tactgggcca cagcgatctt tccaccacgc aaatttatac gcatgtcgct
    41821 accgagcgtc tgcggcaact tcatcaacag catcacccgc gggcgtgatg tctgaaaaga
    41881 acgggaagat ttatgaagaa aggttttatg ttgtttactt tgttagcggc gttttcaggc
    41941 tttgctcagg ctgatgacgc ggcaattcaa caaacgttag ccaaaatggg catcaaaagc
    42001 agcgatattc agcccgcgcc cgtagctggc atgaagacag ttctgactaa cagcggtgtg
    42061 ttgtacatca ccgatgatgg taaacatatc attcaggggc caatgtatga cgttagtggc
    42121 acggctccgg tcaatgtcac caataagatg ctgttaaagc agttgaatgc gcttgaaaaa
    42181 gagatgatcg tttataaagc gccgcaggaa aaacacgtca tcaccgtgtt tactgatatt
    42241 acctgtggtt actgccacaa actgcatgag caaatggcag actataacgc gctggggatc
    42301 accgtgcgtt atcttgcttt cccgcgccag gggctggaca gcgatgcaga gaaagaaatg
    42361 aaagctatct ggtgtgcgaa agataaaaac aaagcgtttg atgatgtgat ggcaggtaaa
    42421 agcgtcgcac cagccagctg cgacgtggat attgccgacc attacgcact tggcgtccag
    42481 cttggcgtta gcggtactcc ggcagttgtg ctgagcaatg gcacacttgt tccgggttac
    42541 cagccgccga aagacatgaa agaattcctc gacgaacacc aaaaaatgac cagcggtaaa
    42601 taattcgcgt gaaacaacag atacaacttc gtcgccgtga agtcgatgaa acggcagact
    42661 tgcccgctga attgcctccc ttgctgcgcc gtttatacgc cagccgggga gtacgcagtg
    42721 cgcaagaact ggaacgcagt gttaaaggta tgttgccctg gcagcaacta agcggtgtcg
    42781 aaaaggccgt tgagatcctt tacaacgctt ttcgcgaagg aacgcggatt attgtggtcg
    42841 gtgatttcga cgccgacggc gcgaccagca cggctctaag cgtgctggcg atgcgctcgc
    42901 ttggttgcag caatatcgac tacctggtac caaaccgttt cgaagacggt tacggcttaa
    42961 gcccggaagt ggtcgatcag gcccatgccc gtggcgcgca gttaattgtc acggtggata
    43021 acggtatttc ctcccatgcg ggagttgagc acgctcgctc gttgggcatc ccggttattg
    43081 ttaccgatca ccatttgccg ggcgacacat tacccgcagc ggaagcgatc attaacccta
    43141 acttgcgcga ctgtaatttc ccgtcgaaat cactggcagg cgtgggtgtg gcgttttatc
    43201 tgatgctggc gctgcgcacc tttttgcgcg atcagggctg gtttgatgag cgcggcatcg
    43261 caattcctaa cctggcagaa ctgctggatc tggtagcact ggggacagta gcggacgtcg
    43321 tgccgctgga cgctaataat cgcattctga cctggcaggg gatgagtcgc atccgtgccg
    43381 gaaagtgccg tccagggatt aaagcgctgc tggaagtggc aaatcgcgat gcacaaaaac
    43441 tagccgccag cgatttaggt tttgcgctgg gaccgcgtct caatgctgcc gggcgactgg
    43501 atgatatgtc cgttggtgtg gcgctgttgc tgtgcgacaa catcggcgaa gcgcgcgtgc
    43561 tggcaaatga actcgatgcg ctaaaccaga cgcgaaaaga gatcgaacaa ggaatgcagg
    43621 ttgaagccct gaccctgtgc gagaaactgg agcgcagccg tgacacgcta cccggcgggc
    43681 tggcaatgta tcaccccgaa tggcatcagg gcgttgtcgg tattctggct tcgcgtatca
    43741 aagagcgttt tcaccgtccg gttatcgcct ttgcgccagc aggtgacggt acgctgaaag
    43801 gttccggtcg ctccattcag gggctgcata tgcgtgatgc gctggagcga ttagacacac
    43861 tctaccccgg catgatgctc aagtttggcg gtcatgcgat ggcggcgggt ttgtcgctgg
    43921 aagaggataa attcgaactc tttcaacaac ggtttggcga actggttact gagtggctgg
    43981 ccccttcgct attgcaaggc gaagtggtgt cagacggtcc gttaagcccg gccgaaatga
    44041 ccatggaagt ggcgcagctg ctgcgcgatg ctggcccgtg ggggcagatg ttcccggagc
    44101 cgctgtttga cggtcatttc cgtctgctgc aacagcggct ggtgggcgaa cgtcatttga
    44161 aggtgatggt cgaaccggtc ggcggcggtc cactgctgga tggtattgct tttaatgtcg
    44221 ataccgctct ctggccggat aacggcgtgc gcgaagtgca actggcttat aagctcgata
    44281 tcaacgagtt tcgcggcaac cgcagcctgc aaattatcat cgacaatatc tggccaattt
    44341 agcgtcatct tctctataaa aaagagcgtg gattgggtac aatcccgctc ttatcaccgc
    44401 attttgacta gctcaataaa agaaatcaga ccatgtttga aattaatccg gtaaataatc
    44461 gcattcagga cctcacggaa cgctccgacg ttcttagggg gtatctttga ctacgacgcc
    44521 aagaaagagc gtctggaaga agtaaacgcc gagctggaac agccggatgt ctggaacgaa
    44581 cctgaacgcg cacaggcgct gggtaaagag cgttcctccc tcgaagccgt tgtcgacacc
    44641 ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc tgctggaact ggctgtagaa
    44701 gctgacgacg aagaaacctt taacgaagcc gttgctgaac tcgacgccct ggaagaaaaa
    44761 ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat atgacagcgc cgactgttac
    44821 ctcgatattc aggcggggtc tggcggtacg gaagcacagg actgggcgag catgcttgag
    44881 cgtatgtatc tgcgctgggc agaatcgcgt ggtttcaaaa ctgaaatcat cgaagagtcg
    44941 gaaggtgaag tggcgggtat taaatccgtg acgatcaaaa tctccggcga ttacgcttac
    45001 ggctggctgc gtacagaaac tggcgttcac cgcctggtgc gtaaaagccc gtttgactcc
    45061 ggcggtcgtc gccacacgtc gttcagctct gcgtttgttt atccggaagt tgatgatgat
    45121 attgatatcg aaatcaaccc ggcggatctg cgcattgacg tttatcgcgc gtccggcgcg
    45181 ggcggtcagc acgttaaccg taccgaatct gcggtgcgta ttacccacat cccgaccggg
    45241 atcgtgaccc agtgccagaa cgaccgttcc cagcacaaga acaaagatca ggccatgaag
    45301 cagatgaaag cgaagcttta tgaactggag atgcagaaga aaaatgccga gaaacaggcg
    45361 atggaagata acaaatctga catcggctgg ggcagccaga ttcgttctta tgtccttgat
    45421 gactcccgca ttaaagatct gcgcaccggg gtagaaaccc gcaacacgca ggccgtgctg
    45481 gacggcagcc tggatcaatt tatcgaagca agtttgaaag cagggttatg aggaaccaac
    45541 atgtctgaac aacacgcaca gggcgctgac gcggtagtcg atcttaacaa tgaactgaaa
    45601 acgcgtcgtg agaagctggc gaacctgcgt gagcagggaa ttgccttccc gaacgatttc
    45661 cgtcgcgatc atacctctga ccaattgcac gcagaattcg acggtaaaga gaacgaagaa
    45721 ctggaagcgc tgaacatcga agtcgccgtt gctggccgca tgatgacccg ccgtattatg
    45781 ggtaaagcgt ctttcgttac cctgcaggat gtgggtggcc gcattcagct gtacgttgcc
    45841 cgtgacgatc tgccggaagg cgtttacaac gagcagttca aaaaatggga cctcggcgac
    45901 atcctcggcg cgaaaggtaa actgttcaaa accaaaaccg gcgaactgtc tatccactgt
    45961 accgagctgc gtctgctgac caaagcactg cgtccgctgc cggataaatt ccacggcttg
    46021 caggatcagg aagcgcgcta tcgtcagcgt tatctggatc tcatctctaa cgatgaatcc
    46081 cgtaacacct ttaaagtacg ctcgcagatc ctctctggta ttcgccagtt catggtgaac
    46141 cgcggcttta tggaagttga aacgccgatg atgcaggtga tcccaggcgg tgccgctgcg
    46201 cgtccgttta tcacccacca taacgcgctg gatctcgaca tgtacctgcg tatcgcgccg
    46261 gaactgtacc tcaagcgtct ggtggtcggt ggcttcgagc gtgtattcga aatcaaccgt
    46321 aacttccgta acgaaggtat ttccgtacgt cataacccag agttcaccat gatggaactc
    46381 tacatggctt acgcagatta caaagatctg atcgagctga ccgaatcgct gttccgtact
    46441 ctggcacagg atattctcgg taagacggaa gtgacctacg gcgacgtgac gctggacttc
    46501 ggtaaaccgt tcgaaaaact gaccatgcgt gaagcgatca agaaatatcg cccggaaacc
    46561 gacatggcgg atctggataa cttcgactct gcgaaagcga ttgctgaatc tatcggtatc
    46621 cacgttgaga agagctgggg tctgggccgt atcgttaccg agatcttcga agaagtggca
    46681 gaagcacatc tgattcagcc gaccttcatt actgaatatc cggcagaagt ttctccgctg
    46741 gcgcgtcgta acgacattaa cccggaaatc acagaccgct ttgagttctt catcggtggt
    46801 cgtgaaatcg gtaacggctt tagcgagctg aatgacgcgg aagatcaggc gcaacgcttc
    46861 ctggatcagg ttgccgcgaa agacgcaggt gacgacgaag cgatgttcta cgatgaagat
    46921 tacgttaccg cactggaaca tggcttaccg ccgacagcag gtctggggat tggcatcgac
    46981 cgtatggtaa tgctgttcac caacagccat accatccgcg acgttattct gttcccggcg
    47041 atgcgtccgg tgaaataagc attacgttat gctcacaacc ccggcaaatg tcggggtttt
    47101 ttatttaagc tgggtaaatg cagataatcg tattctggct tcgcgatttg tcgcctgcat
    47161 caccatccac ggactgaacg cccacggcgt ggcatcaata ccgcgtaata catcagctaa
    47221 atcacaccat tgataatcca tcacttcatc atcgttgatc tgtaacgcac tggtggtgcg
    47281 tgcggcaaat accggacaca cttcattttc cacaatgcct ctcggatcgg tggcgcggta
    47341 gcgaaagtca ggatagatag attcaggagg cgtaatttcc acgccaagct cataacggca
    47401 acggcggatc accgcgtctt cgctgctttc tcccagttgt gggtgcccac aaaccgagtt
    47461 agtccacacg ccaggccacg cttttttgct cagtgcgcgg cgggtaacta ataattgccc
    47521 cttggcatta aacagccaac tggagaacgc gagatgtaag agggtgtctg ccgtgtgtgc
    47581 ggcatacttt tccagcgtac ccgtgggaac tccctgtgca ttcaataaaa tgacgtgttc
    47641 cgtttgcata atttctcaca tgtaattctg atcactcatc attatagata attgaagagt
    47701 gaaatgatta accaagagag cgtttatcga caaccgcccg ttttgagcgg atgccgacag
    47761 gtaaaaacca tttaaaaaag tagcatcata tatttgaagc gactttcaat ggccattgca
    47821 acacagtcag aaatgacagg aatagagaga gtaaaaagca acaacagact gaatatagcg
    47881 cgcattaaaa aattcatatt attctccatg ctcatttttc ttcagcttgc gcatcagaag
    47941 ccacatacgt actgtcctga cttctaaatc tttatcagag acggattcct tcaccaggtc
    48001 agcatgatgc tgatagccat taaagaagac gttcagcaca acggcgctaa gggttgcgag
    48061 cataatgccg ctatgtagca gcggttgcag tacggccggt aacttagaaa agaaatcgtg
    48121 agagagcgtc ggagtcatgc caacgccgag actgatcgcc acaatataga ggttgtaacg
    48181 gttggtggtg tagttacagc gcgacagaat tcgaatccct gtcgccagta ccatgccgaa
    48241 catcactaga ccagcgccgc ccagcacaaa ttgcggaatg gaggctacca gcaccgccat
    48301 ttttggcacc atgccaaaca ggattaatat aattcccgaa gaaatacaca cccagcggct
    48361 atgaacgcgc atcacgctaa ccaggccaac gttttgagaa aatgacgtgt gggggaagct
    48421 gttaaacgtg ccgcctatca ttgtccctac gccatcgaca cgcagcccgc aaataatatc
    48481 gtgtgaagag agtttacgac cgactatttc acccagtgcc aggaacatcc ccatcgactc
    48541 gataaacacg atgattaaca cggcagtcat ggtcagaatg gaaacgggat cgaaaatcgg
    48601 cataccaaac gacatcgggg tgacaatcgc aaaccacgag gcatcatgta gcccggataa
    48661 attgacttca ttcatcatcc acgaaagtaa aaagccaaat acaatcccca gtaatacggc
    48721 gacgttggac ataaatcctt tcgcatagcg agtaatgagc aagataaaaa ttaagacggc
    48781 aaaggagata cctaaataaa cgggattacc atattgcgga ttccctttac ctccagcggc
    48841 ccagtcaata cccacctgaa tgatgctaag cccaatagaa gtaataacca caccggtaac
    48901 cagtggcggg aataaaggca tcaagcgacc aataagtggc gctaataatg tggtgataaa
    48961 acccgcggcg atagtggcac caaatatccc cagcaggccg atatccgggt tcatcccaat
    49021 ggctatcatt ggtgttacag cagcaaaggt caccgacata atcaccggca ggcggatccc
    49081 cataaagcgg ccgataccga tacattgcaa taatgtgacg atcccgcagc aaaagagatc
    49141 cgagctaatg agcatcgcaa tagcatcttt tgagaggccc agtcggtcac caatcataag
    49201 aggaacagcg actgcacctg cgtacatgac aaggacgtgt tgcagaccga gtatgatcag
    49261 ctttcctggt gataatatgc ggtcaacctc atcagttggg cggtcttgcc cagaagatga
    49321 gggaagttgg gaatctatgg cgctcatcag tgtttctcct ttcgcccgcc agaataaaaa
    49381 gcaaccgttt acgttaatga aactgtctaa acacatgctc gtggagtgcc agtttttgcg
    49441 aggaagggga gttagagaca ggaattgcgg aggcaatcac ataatcgtgc ttaaaccacg
    49501 aaaatgacaa gagaaactga caatggaatt atctgtaatt ttctcatatt gatatttcat
    49561 taatcattct ggcatgtcgc actctcgcat ttaatcgttt ttatctg
//
LOCUS       DAJTOW010000018        48123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTOW010000018 DAJTOW010000000
VERSION     DAJTOW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.018"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            188..481
                     /locus_tag="OO244_001142"
     CDS             188..481
                     /locus_tag="OO244_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5663050.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO244_001143"
     CDS             complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO244_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5663051.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1923..2375)
                     /locus_tag="OO244_001144"
     CDS             complement(1923..2375)
                     /locus_tag="OO244_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5663052.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO244_001145"
                     /gene_synonym="yicO"
     CDS             complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO244_001145"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5663053.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3937..5703
                     /gene="adeD"
                     /locus_tag="OO244_001146"
     CDS             3937..5703
                     /gene="adeD"
                     /locus_tag="OO244_001146"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5663054.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCVIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO244_001147"
     CDS             complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO244_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5663055.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO244_001148"
     CDS             complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO244_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5663056.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO244_001149"
     CDS             complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO244_001149"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5663057.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO244_001150"
     CDS             complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO244_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5663058.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO244_001151"
     CDS             complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO244_001151"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5663059.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO244_001152"
     CDS             complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO244_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5663060.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO244_001153"
     CDS             complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO244_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5663061.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13525..13614
                     /gene="tisB"
                     /locus_tag="OO244_001154"
     CDS             13525..13614
                     /gene="tisB"
                     /locus_tag="OO244_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5663062.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13894..15078
                     /gene="emrD"
                     /locus_tag="OO244_001155"
     CDS             13894..15078
                     /gene="emrD"
                     /locus_tag="OO244_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5663063.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15086..15583)
                     /locus_tag="OO244_001156"
     CDS             complement(15086..15583)
                     /locus_tag="OO244_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5663064.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15580..15942)
                     /locus_tag="OO244_001157"
     CDS             complement(15580..15942)
                     /locus_tag="OO244_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5663065.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15932..16279)
                     /locus_tag="OO244_001158"
     CDS             complement(15932..16279)
                     /locus_tag="OO244_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5663066.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16388..16801
                     /locus_tag="OO244_001159"
     CDS             16388..16801
                     /locus_tag="OO244_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663067.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16848..18335)
                     /locus_tag="OO244_001160"
                     /pseudo
     CDS             complement(16848..18335)
                     /locus_tag="OO244_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18325..20040)
                     /locus_tag="OO244_001161"
     CDS             complement(18325..20040)
                     /locus_tag="OO244_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5663068.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20207..21100
                     /locus_tag="OO244_001162"
     CDS             20207..21100
                     /locus_tag="OO244_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5663069.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21097..21911)
                     /locus_tag="OO244_001163"
                     /pseudo
     CDS             complement(21097..21911)
                     /locus_tag="OO244_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21911..23527)
                     /locus_tag="OO244_001164"
     CDS             complement(21911..23527)
                     /locus_tag="OO244_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5663070.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23822..24496
                     /locus_tag="OO244_001165"
     CDS             23822..24496
                     /locus_tag="OO244_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5663071.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="OO244_001166"
     CDS             complement(24535..26196)
                     /locus_tag="OO244_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5663072.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26401..26820)
                     /gene="ibpB"
                     /locus_tag="OO244_001167"
     CDS             complement(26401..26820)
                     /gene="ibpB"
                     /locus_tag="OO244_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5663073.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPA"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OO244_001168"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OO244_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5663074.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="OO244_001169"
     CDS             27650..27982
                     /locus_tag="OO244_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5663075.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29198)
                     /locus_tag="OO244_001170"
     CDS             complement(27984..29198)
                     /locus_tag="OO244_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5663076.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            29299..30399
                     /gene="cbrA"
                     /locus_tag="OO244_001171"
     CDS             29299..30399
                     /gene="cbrA"
                     /locus_tag="OO244_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5663077.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30360..31697)
                     /locus_tag="OO244_001172"
     CDS             complement(30360..31697)
                     /locus_tag="OO244_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5663078.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31772..32920)
                     /gene="dgoD"
                     /locus_tag="OO244_001173"
                     /pseudo
     CDS             complement(31772..32920)
                     /gene="dgoD"
                     /locus_tag="OO244_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32917..33534)
                     /gene="dgoA"
                     /locus_tag="OO244_001174"
     CDS             complement(32917..33534)
                     /gene="dgoA"
                     /locus_tag="OO244_001174"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5663079.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33518..34396)
                     /gene="dgoK"
                     /locus_tag="OO244_001175"
     CDS             complement(33518..34396)
                     /gene="dgoK"
                     /locus_tag="OO244_001175"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5663080.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34393..35082)
                     /gene="dgoR"
                     /locus_tag="OO244_001176"
     CDS             complement(34393..35082)
                     /gene="dgoR"
                     /locus_tag="OO244_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5663081.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35360..36016
                     /locus_tag="OO244_001177"
     CDS             35360..36016
                     /locus_tag="OO244_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663082.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKTLATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36062..36874)
                     /gene="yidA"
                     /locus_tag="OO244_001178"
     CDS             complement(36062..36874)
                     /gene="yidA"
                     /locus_tag="OO244_001178"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5663083.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMAPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36988..37386)
                     /locus_tag="OO244_001179"
     CDS             complement(36988..37386)
                     /locus_tag="OO244_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5663084.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37626..40040)
                     /gene="gyrB"
                     /locus_tag="OO244_001180"
     CDS             complement(37626..40040)
                     /gene="gyrB"
                     /locus_tag="OO244_001180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5663085.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40069..41142)
                     /gene="recF"
                     /locus_tag="OO244_001181"
     CDS             complement(40069..41142)
                     /gene="recF"
                     /locus_tag="OO244_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5663086.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41142..42242)
                     /gene="dnaN"
                     /locus_tag="OO244_001182"
     CDS             complement(41142..42242)
                     /gene="dnaN"
                     /locus_tag="OO244_001182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5663087.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42247..43650)
                     /gene="dnaA"
                     /locus_tag="OO244_001183"
     CDS             complement(42247..43650)
                     /gene="dnaA"
                     /locus_tag="OO244_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5663088.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43947..44027
                     /locus_tag="OO244_001184"
     CDS             43947..44027
                     /locus_tag="OO244_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663089.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44257..44397
                     /gene="rpmH"
                     /locus_tag="OO244_001185"
     CDS             44257..44397
                     /gene="rpmH"
                     /locus_tag="OO244_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5663090.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44414..44773
                     /gene="rnpA"
                     /locus_tag="OO244_001186"
     CDS             44414..44773
                     /gene="rnpA"
                     /locus_tag="OO244_001186"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5663091.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44737..44994
                     /gene="yidD"
                     /locus_tag="OO244_001187"
     CDS             44737..44994
                     /gene="yidD"
                     /locus_tag="OO244_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5663092.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44997..46643
                     /gene="yidC"
                     /locus_tag="OO244_001188"
     CDS             44997..46643
                     /gene="yidC"
                     /locus_tag="OO244_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5663093.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46749..48113
                     /gene="mnmE"
                     /locus_tag="OO244_001189"
     CDS             46749..48113
                     /gene="mnmE"
                     /locus_tag="OO244_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5663094.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11925 a        13022 c        12190 g        10986 t
ORIGIN      
        1 agcggtaagg tcgctgcggt ttaaaaaacg aaacgctatc gataagaata aaaaggaata
       61 aaagtggaat ataaagtctg gcacttctta ctgaccacgc aagcgcgttt cgtacagcat
      121 gatgagagcg atgaaagcaa actacactta tgcttcattc gatacacatt tgtaaaaggt
      181 tgaatccatg aaaccaacga cactactgct tattttcact tttttcgcta tgccaggcat
      241 cgtatacgcc gagtcgcctt ttagctcact gcagtccgca aaagagaaaa ccacggtatt
      301 acaagattta cgtaaaattt gcacgccaca tgcgtcatta tcagatgaag cgtgggaaaa
      361 gttaatgttg tcagatgaaa acaataaaca acatatacgc gaggccatcg tggcgatgga
      421 acgtaataat cagagcaatt attgggaagc gttgggtaag gtagaatgcc cggatatgta
      481 agaacagcaa caactactga ccaacgcttc tttacctgat atcaggattt cttcattttc
      541 acctttgcag taaccaacaa tgcagtcagc aacatcaatg tgccggacaa catcaacggc
      601 gaagtcagac caatattatc cagcgcataa ccgccgattg ccgcgccaca ggtattagca
      661 agctgaataa ccgccacctg aatagacccg gctttttctg cctgatcggc cagcgagcgg
      721 gtgatccacg ttgaccagcc gacgggaacc aatgcaaaag ttagccccca gataatcgcc
      781 acgccggtag caacgatttt atcgcttccc cacaacgtca gtaccaacgc actcacagcc
      841 agtattaacg gcgcgcctgc taaggccagt tttaccgaac gtttaagaat gaacgacgaa
      901 agcgacgtac caataaagct ggcgatacca aaactcaaca gcaccagcgt taagccatcc
      961 acgccgaatc ccgccaggtt catatacacc gggcgaatat acgtgaagaa agcaaactgc
     1021 ccggcgaaag acatgaagat ggcgatcatc cctgccatca cacccggacg ttgtaataag
     1081 cggaaagtat tttgtttctg atgcgagggt tcgcctggca gtgaaggcaa tgatttgata
     1141 atccagaaaa tacacagcac gcccatcacc gccgccgcat taaagacatt gcgccaaccg
     1201 ataagctcgc ctaaaaaact gcccaacggc gcggcaatca ccagcgcaat agaaaccgcg
     1261 ccgaagatca ccgacagcgc cttcggcacc gtacgcggcg gtaccagacg catggtcagc
     1321 gacgccgaca tcgcccagaa cccgcccagc gccagcccca gacaggcacg accgattaaa
     1381 agcaaactga atgagttagc aaaggaaacc agcaagcagg agagcgtcag caaaacggca
     1441 aacaaaataa caacgtagcg gcggtcagtg gcctgaattg tctgggtaat aaacaaactg
     1501 gcaaacattg ccacaaaggc ggtcacggtc accgattgcc cggcaacccc ttccgaaatg
     1561 cctaaatcct gggccattgg cgtcaacaaa ctgacgggca aaaactcaac gataatcaga
     1621 caggcgacac aaaacgccac cgagaaaacg gctgaccagt tcggtcgggt gatggcatcc
     1681 gcgccgcggt tttcggcaat aaattcactc atggtgttac ccgtggcatg tttcagggga
     1741 aaagccgacc actttaacat tgaaatgtgt gacgaattta acgtttttgc aacttttacg
     1801 gtgttgcttg agctggagtt aagatcgaaa cggaacaggg caaagattac cggatgcggc
     1861 gtgaacgtct tatccacccg acaggtcagg cgcggtctgt aggctgataa gacgaaacgg
     1921 tttcaatgca accagtgaac gccctcggca ggctgaaaga acgcgttata gcgcatctga
     1981 aaagcattaa tatcctgcat ttcaggttcg gtttcacgca gaaaacccag cgccagcctg
     2041 acctgagcat cggtaatcgg cgcggaaagg cgggcttttt gtaaaatccg gtgccattgc
     2101 agcaggttct gttcgctatc atcgacaatg acaatttgcc agataagcaa cacctgcgcg
     2161 gcgttttgca ggtgcgactc atccgggcga tgcaaataac gtaaaaattc gtcaccggct
     2221 gactttccca tttgatcgac catcgactcc acgggtacga catcgatatt cagccgatcg
     2281 ctgagacggc gaatcgcttt tctggccccg gatgtcagca cccgaaaacc aaccacaaac
     2341 actaccgcca gcgtggcgag cattatccag atcatgagtt ctcctgcgta atcagaactc
     2401 atgatatctg gaaaccctcg ccagttacta atccaccaga ataatcttca atgcgaacag
     2461 agctgcgacc accaccacac acaggttcag atcgcgccag cgcccggtgc acactttcat
     2521 gatgcagtac gacataaagc cgagtgcaat cccttcggtg atcgagaaag taaagggcat
     2581 catcaccgtg gtaataaacg caggcaccga ttcggtaaaa tcatcccagt taacgcgcgc
     2641 caggctcgaa gtcatcagca cgccaacaaa gattaacgct ccagcggttg cgtaaggagg
     2701 aaccatcgcc accagcggtg agaagaacat aactaacagg aacataacgc cgaccacgac
     2761 agccgttagc cccgtgcgac caccgactgc cacaccagaa gtactttcaa tataggcagt
     2821 aacagacgag gtaccgataa atgcgcctgc caccgaactg acgctatcaa catacagcgc
     2881 cttattcata ttggggaatt taccgttgct atctattaag cccgctttat cagttacacc
     2941 aattaatgtt cctgatgaat caaataggtt gatcagcata aaggagaaaa tgataccggc
     3001 gagttcaagt gttaacgcgc cgctcaaatc tacttcacca atgacgccgc taatatcagg
     3061 cggaatggaa tagacgccgc taaaatgaac atcaccgaaa aataatccac agcaagacgt
     3121 caccacaata gaaaccagca ccgcggcatg aaaatgacgt gatgacaaca cggttataat
     3181 aaaaaaccct aaaataccta acaacacgcc gtgagaactt aaatcgccaa tcatcaccag
     3241 cgtgtcttta ttggcgacaa taacaccagt gttttttaat cccattaagg caataaataa
     3301 tccgattcca ctggtgatac caatacgtaa acttaatgga atgttggaga tcatccagta
     3361 ccggatacga aacagcgtga gcaaaaatag cccaattgcg ccccagaata tcgcgcccat
     3421 cccggtctgc caggagatcc ccatcgcccc cacgaccacg aacgcaaaga aggcgttcag
     3481 ccccattgcc ggagccagcg ccacgggtaa gttagcaaat atccccatcg caatactgcc
     3541 gataccggca atcaaacagg tggtaacaaa caccactttc gggtccattt gtgccgcgcc
     3601 gaggatttgc gggttcacaa aaacaatgta caccatggtt aaaaaagtgg tcatccccgc
     3661 aatcaattct gtgcggacgg tggtcccatg ctgaggtagt ttaaataatc gcgaaagcgt
     3721 ccctgattca ttactcacgt aatcggtatt gtcattattc atttttttgt ccattggagg
     3781 agatttaatc ccttgctttt gtaaaacaaa tgacaacaac ggttcagtga taattatcac
     3841 atttcaattg cacattaata gatattcttt aataatctcg cgacgtttct ttatgataaa
     3901 taataatcaa attgataaaa tcaaaatgag aaaaatatga ataattccat taaccataaa
     3961 tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg tggcgacgct
     4021 gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgc cggagaaatt
     4081 tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga atacgctgat
     4141 actccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat
     4201 gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac cgctaccctg
     4261 ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa
     4321 gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag
     4381 gtcagctctt gcgtgcccgc tctggaaggc tgcgatgtta acggtgccag ttttaccctt
     4441 gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga aatgatggac
     4501 taccctggcg taattaacgg gcagaatgcg ctgctcgata aactcgatgc atttcgctac
     4561 ctgacgctgg acggtcactg tccgggtttg ggtggcaaag aacttaacgc ctatattgct
     4621 gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg gaaattacaa
     4681 ctcggcatgt cgttgatgat ccgcgaaggg tccgctgcca gcaatctcaa cgcactggca
     4741 ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg
     4801 tgggagatcg cccatgaagg gcatatcgat gccttaattc gccgcctgat cgaacaacac
     4861 aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg ccactttggt
     4921 ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct gttgagcgat
     4981 gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg aaccgattga tgcgcaaacc
     5041 ttacaggcgg aagagtcggc gagactggcg ctgtctgccc cgccatatgg caataccatt
     5101 gcccgccagc cagtttccgc cagcgacttt gccctgcaat tcacgcccgg aaaacgctat
     5161 cgggtcattg acgtcatcca taacgaattg attacgcact cccgctccag cgtctacagc
     5221 gaaaatggtt ttgatcgcga tgatgtgtgc gttattgccg tacttgagcg ttacgggcaa
     5281 cggctggctc cggcctgtgg tttgctcggc ggctttggcc tgaatgaagg cgcgctggct
     5341 gcgacagtca gccatgacag ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg
     5401 gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc
     5461 caagtacaaa gtcatctgcc gttacccatt gccgggctga tgagcaccga cacggcgcag
     5521 tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc
     5581 gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc gctaaaacta
     5641 accagtcagg ggctatttga tggcgagaag tttgccttca ctacgctgga agtcacggaa
     5701 taataaaaaa agcccggcgt catgccgggc aaaagtcacc agttacgttt atgccactgt
     5761 caactgctga atttttttct cgcggcggat tttgcgttct tccattaccg ccactatcgc
     5821 catcaggcag atacaaccaa tcgcggcgat atccagcgcg gcgaaggtgc ctgcccagcc
     5881 ggtaaggccg aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct
     5941 gtcaccaatc aggtaagcaa aggtgccttt aataccatcg gcagcgccaa tcgctttttt
     6001 aggtacaaag ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc
     6061 caacgcaaag agagaagcca gatagatata ttcgttactg gcatgttgat acacaccgag
     6121 cgtggcgata atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgccag
     6181 gtcagagagc cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa
     6241 gccctgaatc gccaccgctt tagagagttt cagttcctgg aacgcgtata cggttgacca
     6301 ctggtcgata ccaatacgta ccacatagag gaaaatgttg gcgaagcaca gcagccagat
     6361 cactttgttt ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc
     6421 tgtctctttg tcctcttcgc tgatctcctc gccgaacagt tcttcagctt tgccgaggcc
     6481 ataagattcc ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat
     6541 aatcgacggg aagataaaca tgccgatgac atggccatcg aacaggtaat ttgccccgaa
     6601 cagcgccaca cctgctgcgc ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag
     6661 gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc
     6721 gccggtactc tggaaaaagc cgcttaaggc gtagaaggca atcatcagga acaggctaac
     6781 cgagccgctg cccatactgg cactgaagcc cagcatacaa atggcagaga ggataagcat
     6841 gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag tatgaaacca acgttttacc
     6901 cacgccataa gtgatggaga aacccaggcc gatcatcccc agctgcgtca tgctcaaccc
     6961 gtaggtcgaa atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt
     7021 caggtagccg ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg
     7081 ccgcacttcg agcggaaggt ccagggtcgg cttgcgaact tggtttagga aagccagcat
     7141 gggttactcc tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc
     7201 aggcctgaga tagcgtccaa gaaaaacctg gaaaatttct tagttttgcc ggatatgaag
     7261 tgaaaaggtg agatgcatca cgcttcacgc ggtgtctggg cgttcaaaaa gggcagtaac
     7321 agcagtgcgg aaatcccggc ggcgatagag ataaccacaa aaaatccgct ccagtgccag
     7381 gtatcgagca ctttcgccag cggccaacca gcaagcgacg cccccagata agcaaacaag
     7441 ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccata
     7501 ccaattaaca tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc
     7561 atcacgtagc tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg
     7621 gcgaaaatca aattcatcgg cccacggttg ccgttaaaca atttgtccga gccccaaccg
     7681 gctaccagcg caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg
     7741 accagatcga cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gatcgccgcc
     7801 cggaccacat agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac
     7861 acatatttgg tgaggatctc tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt
     7921 tccagcgcgt cgtgccgcca ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg
     7981 cgtagccgcc agcagagaaa aatccccacg actatcgcca tacaaccagc aatcatcatc
     8041 ccggcacgcc agccgtaatg cagcgcgctc gctgccatca caatgggaat gagtgcgccg
     8101 ccgacgttat gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgtgaatac
     8161 caggccgtta acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc
     8221 acccagagca cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttcatgatg
     8281 cccgtggcga taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg
     8341 ccggagacaa acttcgacac gccgtaagta atgtaaaaca gggtcgctaa caggccgata
     8401 tcgctacggc tgagcacgcc gttagcaagg atttctggta ccgcggcgtt aaaacttttc
     8461 cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag
     8521 tagcgataac gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct
     8581 ttcagaaacg gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca
     8641 cgcgtgccgt gcagacagga aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc
     8701 attccggtga ggccaaaacc ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct
     8761 tcaatcacca gcatcaaccg ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca
     8821 tcggcatgtt tcacaatgtt gttcagccct tcctggcaga cacgaaacag cgtcacgcgc
     8881 tggttttcgc ttaatgctga ttcatcgata cgccattcga gatggctgac aatgccgcgc
     8941 ccttccagct ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc
     9001 agctggcgcg gacgtaaccg tcctaacaaa cggcgcaccg cgtcgtaaac gcccagcgac
     9061 agttgttcga tgagctgccc gctctgcttc acactggcgt tatctgccgc cagccgctga
     9121 acaatgcccg cctgagtacg aatagcagtg atggtctgac cgatatcgtc gtgcagctca
     9181 cgcgccacgt cgcggcgcac gctctcttcg gtttctaaca aacgttcagc cagatgctga
     9241 ttgcgcgcca gttccttttg tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg
     9301 cccagtaaca accctgtcag actttgcacc agcaacgaga gtaataaatc caccagatga
     9361 tcgcgccagg tttgactggc gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct
     9421 ccttgccagc catagtgcca ggccagcgcg ataatcggca gcgccagaca gaatggcgta
     9481 aagcgcgaca gttcgtccgg caatcccaac tggagccaga gactgataac aaacagcagc
     9541 aagtaccaga ccagatgccg cccgcgccag ttgatgggct gagaaaccag tgacgggccg
     9601 agcggcagcc aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc
     9661 gtcagaccgc cagttaaagt cagcaacagc gcattccacg actctttgcc gtgccaaagc
     9721 cagggcagcg actgcaacaa cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg
     9781 cgccagtcac gctgatggcg atagcgcgag atcagcgcta cgggcagtaa cgtcagcaaa
     9841 ctaccgatca tcaataacgg aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc
     9901 agcagccact ccgcacccag caatacgggc cagtatccgc gcgggcattg cagcattagc
     9961 cccagacgca gaccaaacgg aaataacagc accgccatat cagggcgctc aaccagatgc
    10021 aggctgatac tccataggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg
    10081 gtaattaagc gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagct
    10141 ctacgtcgtt actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt
    10201 tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt
    10261 ccgccacctg gcgttcacgt ttggtcagcg ggtcctgacg accggatgcc agtttaatgg
    10321 caatatccgg cgtcagataa cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt
    10381 catccgggct acagcgtttg gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa
    10441 ccagcgcagg actgtcgtga acggagagca taatcgtcgc catacctttc ggcagctggc
    10501 ttagcagctc cagaccggag atatcgggca tggagatatc gcaaatacac acctgcacac
    10561 cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacctgca
    10621 aatcaggttc cagccccagc agctgcgcaa agccggagcg gacgatgagg tgatcgtcta
    10681 taagggcaac ggtgatcatg gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa
    10741 cgataagcgc gatgttgttc aagccttgag cggttactga aaaaacaccg cgatcttgtt
    10801 aaacatcgtc ggatcggact gattacgctg cactttcacg acatcttcca gcttagcgat
    10861 ttggcttatc atctgctcca gacgctggtc gtcattgacc agtagccaga tatggctttt
    10921 gtcgctgtcc tgaatcggca gacaaagaat gccttcaacg ttaaaagcgc ggcgggcaaa
    10981 aaggccacaa acgtgggtca ttacgcccgg atggttgcga acggtgagct ccagaattac
    11041 gttgttatga gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc
    11101 tggcggcacc atcgggtaaa ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg
    11161 gccagggcga ttgatgattt cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc
    11221 acaggtttcg aggccgaatc cggcggcaat ctgcataaag ttgattttgc ccggataggt
    11281 ggcggcaaaa acgccttgct cgtagaacag actctgttgc tgatgcacca gccccagcgc
    11341 ttcgttgttc atcagaatga ttttgacatc cagctgattt tcactggcgg tcgccatctc
    11401 ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt
    11461 cgccagcgcc gcgccaatcg ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga
    11521 ggtcagccac tggcgtgggc gattgagcgg ataagcttgc gcggtccaca tctgatgctg
    11581 acccacatcg gtggtgataa tcgcattgtc atcgacacag gcggcaacgg cgttgatcag
    11641 gccgtaatgg cttaatggat cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa
    11701 atccgctacc aactggtgcc actctgcacg cggttgcgct tccacccgcg ggatcaattg
    11761 cgccagcacg tcatcaacat ccgcctgaat cgccacatgc ggctgcttga ttttacccag
    11821 ctctgcgcga tcgatatcga catgaataat tttggcattc ggacagaact gctcggtttt
    11881 gccaatcgcc cggtcatcaa aacgcgcacc gagcacaatc aacaaatccg cctcctgcaa
    11941 aatatagttg gtactgcgca cgccgtgcat ccccagcata cccagcgaca acggatgcgc
    12001 ttttggcagc atgcccagcg ccattaaagt catggtggta ggcagttgcg ctttctccgc
    12061 cagttcacgc acccgcgcag gcgcattgat cacaccacca cccagataaa gcaccgggcg
    12121 tttggcagcg ttaatcattg tagctgcgtc acgaatgctt tcttcgctaa aggcgggtgc
    12181 aacggctttt tctgccaccg cgggctgagc ttcaatctca aaaaccgccg tttgtacatc
    12241 cttaggaatg tctatccaca ccgggctagg gcggcctgat tgcgcaatgc ggaaggcatc
    12301 gctcatgacc tgtgggagct cttcgatatg tctgaccaga tagttgtgtt tggtgatggg
    12361 gatagagatg ccgtaggtgt ccacttcttg gaaggcgtcg gtgccgatca tcgaggcggg
    12421 aacctgacca gtgatgcaaa tcagcgggat ggagtccagc cgcgcatcag caatggcggt
    12481 caccaggtta gtcgcacccg gcccgctaca ggccatacag accgccggtt taccgtcggt
    12541 gcgcgccatt ccctgagcga taaaccccgc gccctgttca tggcgagcca gaatatggcg
    12601 gatttgcgtg ctttggctta aggcgtcgta aacaggcagg atagaaccgc ccggaatgcc
    12661 cgtcacaatc ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa
    12721 gcgcttacgc gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc
    12781 gactttccgg gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg
    12841 aatcgtgtgt tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac
    12901 gaccactgcg gcggatggcg cagttggtag tagttttgcg ttgaacatgg aagtagtcat
    12961 tggggacctt gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag
    13021 acaatggaaa atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt
    13081 gcagataaca aaaaaccccg ccggagcgag gtttcgtcag ccgcctgcgg ctggtaaccg
    13141 caaagcacac tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat
    13201 ttcggcacga attttgacgt atttagtgca tagttgagta tcgttcacag tttgcgtttt
    13261 gtccaaatat tactgtttat ttatacagta aacttctata atatcactgt acgcaatgtg
    13321 ttatgcgggg gccgcatcgt tacccggcgc actaagtcct ggctgaaacg ggtggtgccg
    13381 tcagcgcctt aaccccgcgt gagcacactg tgttatgtca acaagcacaa cgtttctcct
    13441 tgagataccg cgtgcacaac agctggcaac aggcagcgga aaggtacgtc agctggcagt
    13501 gctcctgaac cacaggagac gcgtatgagc ctggtggata tcgccattct tatcctcaaa
    13561 ctcattgttg cagcactgca actgcttgat gctgttctga aatacctgaa gtaattcaga
    13621 ttcaagtcgc accaaagggg agcgggtaac cgctcccctt ttatatttag cgtgcgggtt
    13681 ggagtcggat gcgatgctga cgcatcttat ccgccctacc atccctcccg gcaacattta
    13741 ttgccgcttt tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg
    13801 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
    13861 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
    13921 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
    13981 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
    14041 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat atccgaccgt
    14101 gtcggtcgcc gaccagtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
    14161 gcggtcacga cctccagttt gacggtattg attgccgcca gcgcgatgca ggggatgggc
    14221 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgtg atttatatga acggacacag
    14281 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
    14341 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
    14401 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
    14461 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
    14521 aactgttatt tgctgatgct gattggcggt ctggccggga ttgctgcctt tgaagcctgc
    14581 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
    14641 attctgccga ttccggcggc attttttggg gcatggtttg ccgggcgtcc taataaacgc
    14701 ttctcaacgt tgatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
    14761 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
    14821 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
    14881 ttcctggcgg gcacggctgg tgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
    14941 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
    15001 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
    15061 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
    15121 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
    15181 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg ccgagcgtgc gggcacaggc
    15241 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
    15301 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
    15361 actcaaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
    15421 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
    15481 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagct
    15541 tatgcctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
    15601 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
    15661 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
    15721 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgaggatgcc
    15781 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
    15841 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
    15901 ccctgggtcg gcaatgcgtc tggctttacg gctatccggc atacaacacc agtcccataa
    15961 cgatcaccgc cacaaccatc aatattaagc tgatgattaa taagctgttg gtgtagggca
    16021 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacattg
    16081 ctaacccgcc ggaaaacaga cacaacaaca acgccagcag ttcacgaatg acgggcgtgg
    16141 cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
    16201 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
    16261 caaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
    16321 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc cgctacaagg
    16381 aggtcagatg ggtatcatag cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc
    16441 catcgcttta atgatgggga ttatccactt ttcattcggc cctttttccg caccaccgcc
    16501 aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg
    16561 aataaaaggc gaaaaaatta ccactgtgga gaaaaaagag gacatggata tcgataaaat
    16621 cctcgatcaa agcggtatcg cactggccat tgctgcgcta ctttgcgcat ttattggcgg
    16681 tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg gtttttggta tcggcatcgt
    16741 gtgcagcatt ttgctgatat tcctgatttt ttccttcctg accggcggtt cgctggttta
    16801 agcagcctga ataatgcccg ccgggtgatc atcagcgggc attatcgtca gaatttctgt
    16861 tttttctcct ccaccttcac tccttgcgtc ggtaggccgg tgtcatagtc gcgtaccact
    16921 ggcgaatagc catcttgtgg gcgtgggcga aacgccccca tccagcgcgg tcgtgcatct
    16981 ttacgccacg gacgcagact ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg
    17041 taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc
    17101 aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg
    17161 ttgagcacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag
    17221 ctgtcatgct caatctcgta gcggttaaat tccaccatca cgccgcgtgg ctctttcacg
    17281 gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt
    17341 gtcggcagta aatcaatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc
    17401 gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc
    17461 agcttatgtg cgcccatcat ttcaccgtga tcggaggtat aaatgaccca cgtattttca
    17521 cgttgctctg gcgttaaggc attgatgaca cgtccgattt ggtcatcaac aaagtcattg
    17581 caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc
    17641 tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgtga tttctcgcca
    17701 agttcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga
    17761 tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcac gggctattgc
    17821 agaaaatcta ccgctcgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg
    17881 ttcgcctgta aatcttcgac gctgtttagg ccattgcgcc acaggctgat ctctttttcc
    17941 gtcagttcgc taagatagtt cgccccatca aaccagtaat cagcatccca ctccggcgga
    18001 cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg
    18061 tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg
    18121 ttgttggtcc acgggccgga ctggttagcg tagataccgg tgaatagtcc gacccgtgca
    18181 ggcgtacaaa ccggtgaaca agtgtaggcg gaattaaagc gaataccttc cgccgccaga
    18241 ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgacaat attggtggcc
    18301 tgggtatcgg tcaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac
    18361 ggctgggtcg tgacgatgcc accagctgtc aaaaatcagg tagacaatca ctacggcagc
    18421 gatgaaataa ctgatcatcg ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc
    18481 gacatagacg ccaatcatcg cgaacaggat accaattgac gcgatcttga cgtttttcca
    18541 cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat
    18601 aaaaccgatc accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag
    18661 gaaatggaag tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc
    18721 catcgccact tttgccgcca gcgccgggat gcgcggaaag aaaaagccca taatgatgat
    18781 ggtaaccagc ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg
    18841 cggcgcgttg gcgatccacg gcgctaccaa aaccgaaacg atagcgataa agaaaccaaa
    18901 tttgcgcccg acggtgacca gctgctgcgg ctcggcattc tggttaatga tgcgacggta
    18961 aatacccata ctgaataatg tactggcgct attcagaaag ccgttgaagg tactgatcac
    19021 cgcaccaaat aacaccgcgc cgaagaaacc ctccagtggc actggcagaa cgttattgac
    19081 cagcgtcggg taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa
    19141 ccctggcagc accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc
    19201 cttttgccct tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt
    19261 acaccagtaa aaggtgttca ccagaattaa accggtaaat gccgcgccaa tcggcaaggg
    19321 atcggtcggg ccaccgactg agtttaattt ctcggcgtga acggtggtaa tttgctcaat
    19381 gccctgcata aagctgccct tgcctatcgc gatcaggcca aataccggca ccatcaaccc
    19441 gccgataacc agcccaatac cgttgatgga gtccgccact gccattgcgc gcagtccgcc
    19501 gatcaccgca tacaaaattc ccgccagacc aagcaaaatt accagtagcc agatagccgc
    19561 accgtgagaa atctgtagcg attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc
    19621 ggagtagaga acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat
    19681 gataatacgc gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc
    19741 gcgcttcaga tagcgcggta aaaagattag cgcgaggaag atcagcgtca ccgctgaagt
    19801 cacttcccag cccatcaccg acatgccgct tttgtaggcc tgcccggaaa gaccaaccag
    19861 ttgttccgtg gaaaggttgg ttaacattaa cgaagcggca ataaccggcg cttttagtga
    19921 acgtccggca agaaaatagc cttgttgcga tccggtatct gttttgcgaa ccttccacca
    19981 ggtgatcacc gccaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat
    20041 ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc
    20101 gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt atccctgaac ggattgtttt
    20161 ctgttaagtt caggttgtga gcataattcg ccggagggat attaaaatga atggaaaatt
    20221 gcaaagttca gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa
    20281 tgtgatctcc agcggaattt ctctgatctc gctctggcat acctacgccg acgagcatta
    20341 ccgggtgatc tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt
    20401 gtataccgta caaggatgtg ggaaaattct tttaaagaat ggcgaacaaa taactctgca
    20461 tagcaactgt attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt
    20521 agtctgggaa cagtactgga tggaatttac ccccaccagt atgatggata ttcccgttgg
    20581 tcagcaaagc gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga
    20641 gttaataact tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat
    20701 tatttatcag tggatttgtc ttatgtccgc agatggtaaa aaagatccac aacggcggca
    20761 aattgaaaaa ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga
    20821 tatggctgcc acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac
    20881 cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt
    20941 aaagcaacaa ggaaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt
    21001 tcatttcagc aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa
    21061 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
    21121 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
    21181 tttcgacaac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
    21241 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
    21301 gccgaccacc agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc
    21361 ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt
    21421 ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg gttggagtag
    21481 ttgagcatct aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg
    21541 ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaaca
    21601 ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg
    21661 tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac
    21721 gcaatgtccg gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc
    21781 cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc
    21841 aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag
    21901 aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa
    21961 ttaagctatc gagctgttca cgcaattggg atacatgcag accaatgatc acctgaatgg
    22021 catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg
    22081 tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc gcaattgtta atgctggaga
    22141 tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg
    22201 gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca
    22261 cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa
    22321 ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg
    22381 cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctccgc
    22441 ccatgttgcc caccacacca aagagataca ttacggtcga cattgaggcc gccagcacgg
    22501 cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa
    22561 ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact tttacccgat
    22621 tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg
    22681 agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagtggc tcggcgctca
    22741 gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca acagctgccg
    22801 gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt
    22861 cgaggaaggt gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag
    22921 attcaatccc catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca
    22981 tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg agaaaaaccg
    23041 gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg
    23101 cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg
    23161 ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat
    23221 tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca cgtccctgcg
    23281 cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata
    23341 ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg
    23401 tcagtgattc cccgacaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcag cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaattcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 ttacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtgcctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggtg
    29161 agcaggtgat tacggggagc aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg
    29221 gttcccgtca tcatacttca caaggacttc actttcagac aatagtcttt tgccatcgtg
    29281 atgaatgtga gtgaaaagat ggaacatttc gacgtggcga ttattggcct cggcccggca
    29341 gggtcggcgt tggcacgaaa gttagccggc aaaatgcagg tgatcgcgct ggataaaaag
    29401 caccagtgcg gtactgaagg tttcagcaaa ccttgcggcg gtctgctggc accggacgcg
    29461 cagcgatctt ttattcgcga tggactgacg cttcctgtcg atgtgatcgc caatccacag
    29521 attttcagcg tcaaaactgt cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc
    29581 tatatcaata ttaatcgcca tgctttcgac ttgtggatga aatcgctgat ccccgccagc
    29641 gttgaggttt atcacgatag cctgtgccga aaaatctggc gtgaggatga taaatggcat
    29701 gtcatttttc gtgcagacgg ctgggagcaa catattaccg cccgctatct ggtcggtgcc
    29761 gatggcgcaa actcgatggt gcggcgacat ctctacccgg atcatcaaat ccgtaaatat
    29821 gtcgctatcc agcagtggtt cgcagagaaa catccggtgc cgttctactc ctgcatcttt
    29881 gataatgcga taactgactg ttactcatgg agtatcagca aagatggtta ttttatcttt
    29941 ggcggtgcct atccaatgaa agacggtcag acgcgtttca cgacgctgaa agagaaaatg
    30001 agcgcctttc agttccagtt tggcaaagcg gtgaaaagtg agaaatgcac ggtgttgttt
    30061 ccctcacgct ggcaggattt tgtctgtggt aaggacaacg cctttctgat tggtgaagcg
    30121 gcgggattta tcagcgccag ctcgctggag gggattagct atgcgctgga tagcgcagag
    30181 attctgcgtg cggtgttcct gaaacagccg gagaagagca acgccgctta ctggcgcgcc
    30241 acacacaaac tgcgtttaaa actcttcggc aagatagtaa aaagccgatg cctgaccgca
    30301 ccggctttaa gaaagtggat tatgcgcagt ggtgtggcgc atattccaca gttgaaagat
    30361 tatccaacgc gcttcacatc acccaccagc aggatgtaag agagcgcgcc aatcaacgcg
    30421 acggcggaga tataaaccag tgcaggcgcg aaaccgtaac cctgcgccag gtaccccacc
    30481 accagcggaa cggtgatgcc gcccagacca ccggcgaagt taaacacgcc gccggttaaa
    30541 ccaatcaggc gcatcggtgc cagagaagag accagcgacc aggtaatcga agcaaaaccg
    30601 ttgccgaaga atgccagcgc catcaggcac ataatcatca tcggatcgtt ggtgtagtta
    30661 gcgcccataa tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag
    30721 cccagtgaaa agcccttacg taccagcaga tccgcgaccc agccagagag caggacgccg
    30781 acaaacgccg cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc cgtgattcct
    30841 ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa accacagtgc agaagccacc
    30901 gcaaattgcc caagataaac gccgatcagt ttacgatgga acaccagttt ccagtctttg
    30961 gctgttaacg gctgacgcgc ctctttcttc accggcgcat caccatccac cagaccgccg
    31021 ccatcacgaa tgtaatccag ttcagctttg ctgatacctt tggtcaggcg cggcggctga
    31081 taaaccttaa accaaatcag cgaccagata atgccgatac caccagtgac aatgaacacc
    31141 cagtgccagc tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg
    31201 acaaactgac cagacgtata aaaaccaacg gcggaagcgc gttcatgttc cgggaaccag
    31261 ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca
    31321 cgcagaccaa ttaatgacat taatcccgtg gcaaagcctt ggaataaagt cgccactgac
    31381 caaccaaata tcgcaataaa ataagtcacg cgagaaccta cgcgatctaa aaaccaaccg
    31441 ccggggatct ggcatagcgt ataaagccag gcgaaggccg aaaatacata gcccatttcc
    31501 gctttcgtaa tgccgaactc ttcctgaata tgggcggaag ccacggccag gttagcgcgg
    31561 tcaacataac aaatgaccac cgtaataaag atcatcacca gcgtcagata acgccgacgc
    31621 cccggctttg ctgcattaac gggaatatcc atagcgagct ttctccagat tttgggcata
    31681 gcgaagccgc tcaccatgcc ctgtaattta cagagggtta tatttttgta ttgctgtttt
    31741 agtgcccgat gaggggctta cgtggcagga attaccactc tgctacgctg ttatcttcat
    31801 gacgccagag cggattacgc cagtccgggg catttttact gaactcaata actttagctt
    31861 cgtcgatttc cacacctaag cccggtttcg ttaacggttt aaagaagccg ccaaccatgc
    31921 tgaagtcttc tttgtttttc acaaagtcga gtaactccgc gcctttgttg taatgaatgc
    31981 ccatactttg ttactgaagt accgcgttat aggaaacaaa gtcgatatgc aggcaagccg
    32041 ccagtgcaat cggtccgaac ggacagtgcg gcgcaagggt cacatcatag gcttctgcca
    32101 ttccagcaat tttgtagcat tcggtaatac cgcctgcatg ggagagatcc ggttgcagaa
    32161 tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac
    32221 ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg gtagtattcg gcttgttccg
    32281 ccagcaccgg ctcttcaata aacagcgggc gatacggctc cagttcttta atcagcactt
    32341 tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc aaactcaatc tgattgccaa
    32401 aagcttcacg aatttgtgcc acggtgttga ctgccgcatc taccgcgcgg gagttatcaa
    32461 ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc gaagccgatt tcgcgcaggg
    32521 ttttaatgcc gtcgataaca tccgccggac gatcgccgcc gacccaactg taggctttaa
    32581 ttttgtcgcg aaccaggccg cccatcagtt gccagactgg cgcattcagc actttgcctt
    32641 tgatatccca taacgcctgg tcgatcccgg cgatggcgct catcaggatt ggaccgccac
    32701 gataaaatcc ggcgcgatac atcacttgcc ataagtcgtt gatgcgcgaa ggatcctgac
    32761 caatcaaata gtcacccagc tcgtgaactg ccgcttccac cgtacgggcg cggccttcaa
    32821 tcacgggctc gccccaaccg accacgcctt catcggtttc aattttcagg aacatccagc
    32881 ggggaggtaa acgatacgtg gtaattttgg tgattttcat tgcactgcct ctcgatacgc
    32941 cttaacaaat gctgctgcct gctgcgcggt gcgttctacg gactgcccgg cgcgataaag
    33001 atcgctgcct aagcccgccc caacacaacc tgcgtctatc cactgcgcca ggttttctgg
    33061 cgtcacgccg ccaacggcaa agactgcgat gtccggtggt aataccgctt ttaacgcttt
    33121 gatgtattgc ggaccaaaag ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc
    33181 gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg caaacggtca tgccgtagcc
    33241 caccgcgcgg cggatcactt cactatggat attgggcgta acgatgagct gacagcccat
    33301 cctggcgagc gcatcgacct gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt
    33361 gtcgccatac gcatcaacga tggcgggaat gctctgctcc cattgtgggg aattcagcgg
    33421 gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc
    33481 gggcgtaatg ccgcgcaaaa tggcgatcag cgggagttta gtttgccact gcatgagcga
    33541 tgctccttat accagcctga aatgccgtgt cgcccgccac cgccgccacg tcgcaaccca
    33601 tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt tccggcgaca agggtgatgg
    33661 cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc tgcgccaatc aacaaaccag
    33721 agagaaattc gctgacctgt tcgcggggaa gtgttcccag cacatgcgag gcgcgaactt
    33781 caaaaagctg cggcaatatg gcgggcgcat taaggccgcg ctcaaggcca gccgcgaagg
    33841 catcggcaga gttttcctgc ggcggcaaac ctgcgccaat caatgagtga tttaacagta
    33901 aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct
    33961 gcacccattt gcaatgcgta ccgggcataa cataaagaga ggaaggagcc agagctcgcg
    34021 cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg gttatcgtca tgagagacgc
    34081 ataatccggg aataatccag atattgtcgc caacagacgt taattgttcg ccaatagacg
    34141 aaaaacgggc aggaacagat aaatacggag caactttcca gccgacattg ctgccgacca
    34201 ttcctgccat caccactggc gtattttctt cacgccagtc ggtcgtgact tctgctaaca
    34261 ctgcagccgg agattttccg ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca
    34321 ggcagtggtc gccctgataa agccaggcgc gcagattggt cgatccccag tcaattgcga
    34381 tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc gagctggcaa tcatggtaag
    34441 cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg
    34501 ttcctggagc gtttgcggca tgttggcctc atcgcccatc caggttcgtt caaaaaccgc
    34561 ccgctgcagc gaactgatcg caatgctaag ttgctgtaac accgggttat gcaccgactg
    34621 cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt
    34681 ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga tcgctggagg tcgcgcgctc
    34741 tgctgcccaa cgggcaatcg ccggttccac cagatttcgc acttcgctca tggcactgat
    34801 aagccgtggg tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt cgaggtaatt
    34861 ccactggtta cgcggtgcca caaacgcccc gcgataacgt ttcatttcaa tcagccgctt
    34921 cgccatcagc gaacggaaca cctcacggat gatgttgcgc gaggttgcaa actcttcaca
    34981 gagctccgcc tcagccggaa gtggcgagcc aggtacgtat ttgccgtgaa cgatctgttt
    35041 acccagcgta atgacaatgc gatcggtttt attgagagtc atggagagtc cttgtgctct
    35101 gtatgttctt ctctacttta ccccgatcga tgcataacgc ggcaactttg tagtaccagc
    35161 gtgatgacgt tcgcgtttgc cgcgcgcgta atgtagtaca aacttatatt gttgtgctac
    35221 aatttagatc aaaaaaagaa caacacataa aaaaatgaca tgcgtcgggc agaaatctga
    35281 aaagggatat caggcgctaa acaggaggga aagaagagta tgctttcaac ggcttagcta
    35341 ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga ttttttaagg ctgccggttt
    35401 attcccactg gcgctgatgc tttcaggctg tatctcgtat gctctggttt cccataccgc
    35461 aaagggtagt tcaggaaagt atcaatcgca gtcagacacc atcactgggc tatcgcaggc
    35521 aaaagatagt aatggaacaa aaggctatgt ttttgtaggg gaatcgctgg attaccttat
    35581 cactgatggt gccgatgaca tcgttaagat gctcaatgat ccagcactta accggcacaa
    35641 tattcaggtt gccgatgacg caagatttgt tttaaatgcg gggaaaaaga aatttaccgg
    35701 cacaatatcg ctttactact actggaataa cgaagaagaa aagacactgg caacgcatta
    35761 tggttttgcc tgtggtgttc aacactgtac caggtcactg gaaaacctaa aaggcacaat
    35821 ccatgagaaa aataaaaaca tggattactc aaaggtgatg gcgttctacc atccatttaa
    35881 agtgcgattt tatgaatact attcacccag aggcattccg gatggtgttt ccgcagcatt
    35941 actgccagtg actgttacac tggacatcat tactgcaccg ctgcaatttc tggttgtata
    36001 tgcagtaaac caataatcag taagcgggca aacgcgttta tgctgtttgc ccgcccacag
    36061 attaattcag cacatacttc tcaatagcaa acgccacgcc atcttcaagg ttggattttg
    36121 tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc
    36181 ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg atttcttccg
    36241 gtttaatacc taacacgtcg gccagtgatt tcacacccgt acctttgtta acgcgtttat
    36301 cgaggatttc gaggaagtac ggcgcacttt tcagcacggt atatttctct ttcacttcct
    36361 gcggaatacg cgcgatagcc tggtcgagga tggcgggttc atcaatcatc atcactttca
    36421 ggaactgggt attgggggcc attttctccg cttcgcagaa caccagcgga attgtggcaa
    36481 cgaaggattc atgcaccgtg tagtagctga tatcgcggtt agcggtgtac agcgtggtgc
    36541 ggtccagggc gtggaaatga gaaccgactt cgcgagagag tttttccagg aaacgatagt
    36601 cgtcatagct gagagcggtt tgcgccacgg tgctaccatc agcggccttc tgtaccagcg
    36661 cgccgttata agtaatgcag tagtcgcccg gctgttccat atgcagctct ttcaggtagt
    36721 tgtgcacacc tgcatacgga cgacccgtcg ttagcacgac attcacgcca cgggcgcgag
    36781 ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggcagc agaagggtgc
    36841 catccatatc gatagcaatg agtttgatag ccatgagttc ccaaggtaga ttagttcctg
    36901 acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa aaggggatgc
    36961 aacgcgggtt gcatgctccc catttgctta gcggaagagt ttccctttca gcagttccat
    37021 gcctgcggaa agcagatcgt tattggcttg tgctgacact tcaccttgcg gtgagagcgc
    37081 atcaataatc ttcggcaatt gctctgccag taaactggaa gccgtactgg tatccacgcc
    37141 aagtttttgc ccgagatcgg acaccgcatt tgtgccgagc gccgactcca gttgctcgcc
    37201 gctaactgat tgatttctct gttgattact caaccaggtt gagagaatgg cccctaagcc
    37261 gccactttgc agtttttcca gcagcacctg aatgccaccc tgctcctcaa cccaacttaa
    37321 aatcgcctga tatttccccg catcgccttt cagaaaggca ccgacaactt catcaaaaag
    37381 ccccatgata atcacctgta aagcgttacg tgttgaccca aaaagtatag atctgcggga
    37441 tgataattgc ggattgcaga aataaaaagg gcggagatga tctccgccct tttcttatag
    37501 cttcttgccg gatgcggcgt gaacgcctta tccggcctac aaaatcatgc aaaatcaata
    37561 tattgcagga gtctcgtagg actaataagc gtagcgcatc aggcacgctc gcatggttaa
    37621 cgccattaaa tatcaatatt cgccgctttc agggcgttct cttcaataaa cgcacgacgc
    37681 ggttctacgg cgtcgcccat cagcgtggtg aacaactggt cagcggcaat cgcatcctta
    37741 acggtaacgc gcagcatacg acggctttcc gggtccatgg tggtttccca cagctgttcc
    37801 gggttcatct cgcccagacc tttataacgc tggatggaga ggccacgacg ggactctttc
    37861 accagccagt ccagcgcctg ctcgaagctg gctaccggct gacgacgctc gccacgttcg
    37921 ataaacgcat cttcttccag caagccacgc agtttctcac ccagcgtgca gatacgacga
    37981 tattcgccac cggtgataaa ctcgtgatcc agcggatagt cagtatccac accgtgggta
    38041 cgcacgcgaa caatcggctc gaacaggttt tgttcggcat tggtgtggac atcaaacttc
    38101 cactggctgc cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg
    38161 gtaacggtct gctcatcaga aaggtcagct tccgtcaacg tcggctgata gataagctct
    38221 ttcagcattg ctttcggata acgacgctcc atgcggttga tcattttctg cgtcgcgttg
    38281 tactcagata ccagtttctc taacgcttcg ccagccaatg ccggtgcact ggcgttggtg
    38341 tgcagcgttg cgccgtccag cgcgatagag atctggtact gatccatcgc ttcgtcgtct
    38401 ttaatgtact gttcctgctt gcctttcttc actttgtaca gcggcggctg agcgatgtag
    38461 acgtgaccgc gttcaacgat ttccggcatc tgacgataga agaaggtcaa cagcagcgta
    38521 cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt
    38581 ttgtccgggt tgtactcgtc acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc
    38641 acttcctgag aagagagcat cttatcgaag cgcgctttct cgacgttaag gattttaccc
    38701 ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg
    38761 gagtcccctt ccaccaggta cagttcggaa agcgccggat cgcgttcctg gcagtctgcc
    38821 agtttgcccg gcaggccagc taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg
    38881 cgacgcgccg cttcacgggc acgggcagca tcgataattt tgccaaccac gattttcgca
    38941 tcagttgggt tttccagcag gtattccgcc agcagttcgt tcatctgctg ttcaaccgcc
    39001 gatttcacct cagaagaaac cagtttgtct ttggtctggg aggagaattt cgggtccggc
    39061 acttttacgg aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt ggcgctgact
    39121 ttggcttttt tgctgtagcc ttctttgtcc atgtaggcat tcagggtacg ggtcatcgcc
    39181 gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag
    39241 tagatgtttt cctggaagcc atcgttccac tgcagcgcca cttcaacgcc aataccgtct
    39301 ttttcggtgg agaagtagaa gatatttggg tggatcggcg ttttgttctt gttcagatat
    39361 tcaacgaacg ctttgatgcc gccttcatag tggaagtggt cttctttgcc gtcacgcttg
    39421 tcgcgcagac gaatggaaac gccggagttg aggaacgaca actcacgcag acgtttcgcc
    39481 agaatttcat attcgaactc ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc
    39541 accatggtgc cggttttttc agtctcgccg gtaaccgcca gcggagcctg cggtacaccg
    39601 tgttcgtaga tctgacggtg aattttaccc tcgcgctgaa taaccagctc cagtttttgc
    39661 gacagggcgt ttactaccga aacaccaacg ccgtgcagac cgccggacac tttataggag
    39721 ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga tcacttccgc cgccgatacg
    39781 ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac agagacagag
    39841 ttatcggcgt gaatggtgac gataatttct ttacagtgac ccgcgagcgc ttcgtcgata
    39901 gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg
    39961 atatacatac ccgggcgctt acgcaccgca tccagccctt tcaggacttt gatactggag
    40021 gagtcataag aattcgacat caacgtttct cgctcattta tacttgggtt aatccgttat
    40081 tttacccttt tccacggtaa acatcttcga attttcgtcc gacatgtcta taacgtgttc
    40141 agcactgatc gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc tggcaagcaa
    40201 cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct atcaggtaga gacaccgccg
    40261 cccgctttca cgagtgagga actctccttg cgccagacgt aaggcgcaca tcaacagctt
    40321 aagctgccca cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc
    40381 cgctttatgc gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa aattacgttc
    40441 cagcacctca gcgtactctg tctctttctc ccagccgcgc tggaaagaga aagtcagaga
    40501 aaactcaggg agaaattgct tacaggtatc atccatatcg gccgcgatac cggcgctata
    40561 ctccgcgcgc caggtgctga tttgctccgc cagcgggatc agttctttat cccacgggcg
    40621 tagctgttcg taacgagtca cctggcgcag cgccgcattg cgctgcttga gcaatcgctt
    40681 gagattgctc caggcggtga aaaatccagg ttcgttgtga aagcatcccc agtcgaggaa
    40741 tgctcttctg tatttggggc cgccgttgag taaagtaaac ccttctggcg ttatcaactg
    40801 catcggcatc aggtgcgcca gttccgcgac tttatgcccg tccgtaccgt caatacggac
    40861 tttgctgtcg ccctgtttgt ctttggttaa gccaatcgcc gtctcgcgct cttcgccctg
    40921 taatcgcccg tggagaacaa atgcctcctg ctcatggcga atgacgcgac caatctgcaa
    40981 actgcgaaac gcccgaccat ggccgagcgt atagatggct tccagcacgc tggttttgcc
    41041 actgccgttg gcacctacca gaaagttaaa gccgggagat aaagcgagat ccgcggtttc
    41101 aatattgcgg aaatcgcgga tcaacaagcg ggtgagggac attacagtct cattggcatg
    41161 acaacatagg cagccgactg tgatgcggca tcttcaatct gcacgctgga aaccgaatcg
    41221 gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca gaacatccag cacatagctg
    41281 acgttgaagc cgatttccat ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct
    41341 tcttcctgtt ccgggttgtt ggcagtaatt ttcagctggt tttcgctgac atacaggcgc
    41401 acaccgcgga atttctcgtt agagagaatc gccgcgcgag caaacgcctg cttgagcaga
    41461 tcgcagcctg cttccagatg tttgtccggg ttcttcggca gaacgcgacg gtaatccggg
    41521 aagcgaccat ccaccagttt cgaggtgaaa ataaagtcgc caacgtgggc acgaatgtta
    41581 ttgctgccaa tctgcacacg cagcagattg tcgccgccgt cgagcatacg catcagttca
    41641 atcacgcctt tacgcggtac gatcaccgaa tggcttggca aagattgacc aattggcatt
    41701 gaacagaccg ccagacggtg gccgtcggtt gccacggtgc gcagttcttc accttcggtt
    41761 tcaaacagca taccgtttaa gtaatagcga acatcctgat gcgccatcga aaactgggtt
    41821 gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc gctctgccag
    41881 tcatcgaggt tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg gctacgcccg
    41941 gagcgcacca gcatccgttc accttccagc tgcacggcaa tttccgcgcc ttcaggcaga
    42001 ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc
    42061 tgaaccagcg caacgcgtgc caccatttcc atctcgagat cagtaccggt cagcgacaac
    42121 gtaccgtcag caacctgtaa cagcagatta ccgagaatcg gtagcgtagg acgaccacct
    42181 aacggaccgc tcacctgttg tagcggtttt aataaatgct cacgttctac ggtaaatttc
    42241 ataggtttac gatgacaatg ttctgattaa atttgaaaaa tcttctttga tatcgtggct
    42301 ctcttcacgc aactgctcga tcttacggca ggcatgaagc accgtcgtgt ggtcacgccc
    42361 accaaacgca tcgccaatct ctggcagact gtggttagtc agctctttcg ctagcgccat
    42421 cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac
    42481 tttgatcttg tagtactccg ccaccgtctt ctgaatattg tcgatggtga ccagtttttc
    42541 ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag tcgatggtga tcgcccgtcc
    42601 ggtaaagttg gcgttagcaa taacgcggtt cagcgcccct tccagctcac gtacgttaga
    42661 tcgtagacgc ttggcgataa agaacgccac ttcacccggc aaacgaatgt cattttcgtc
    42721 ggcctttttc atcaggatcg ccacacgggt ttccagctct ggcggttcga tcgccacagt
    42781 cagtccccaa ccgaagcggg atttcaaacg atcctcaacg ccgttgatct ctttcggata
    42841 gcgatccgag gtgagaatga tctgttgatt accttccagc agggcgttga aggtgtggaa
    42901 aaactcttcc tgagatcgtt ctttattagc aaaaaactga atatcgtcga tcagcagtgc
    42961 atctacggaa cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca gggctttaac
    43021 catgtcctga acaaagcgct cggagtgcat ataaaccact ttggcattcg gcttgcgcgc
    43081 cataatgccg ttacccaccg catgcagcag gtgagtttta cccagacccg tgccgccata
    43141 aaggaacaac gggttatagg caccgccagg gttatccgcc acctggcaag ccgccgcgcg
    43201 cgccagttgg ttagatttac cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt
    43261 agaacgatag gtcggttctg ccggggccgg gacgttatcc caacctgagc gcgtagaagg
    43321 cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac
    43381 tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca gctgtggggc
    43441 atccgctccg cagaaactgg ttagcagtcc attgatatta ttaaggtact tgtcccgtac
    43501 ccaatcgagg acaaaacggt ttggcgcgta cagggccagc gtgttatcgc tcagttccgc
    43561 ctgcaatggg cgtatccaca tactgaattc tgtggctggt aactcatcct gcaatcgggc
    43621 aagacactgc tgccaaagcg aaagtgacac ggcggactcc actcgaacaa aagtcgataa
    43681 tgactaaggc tgaaacattc atgattgttg acgtacgtcg aaaagaccct gcttgagggt
    43741 gacgcacgaa ccgctgtctg cggttatatg cccgatcaag atcctgcaaa acgatcggga
    43801 ccgcggatca tagcctaaac tgcgcaagag atcttctgtt tctcacagat tttccctatt
    43861 tatccacagg actttccaga actcgctaag tgtaaacgat cctgccgcga ggcgggcacg
    43921 atttacgccg catattggaa aatttaatga ccatagacaa aaattggctt aatcgatcta
    43981 ataaagatcc aggacgatcc ttgcgcttta cccatcagcc cgtataatcc tccacccggc
    44041 gcgccatgct ggtttccact ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa
    44101 aagcgcggta aataaggaaa gagaattgac tccggtgtgt acaattatta caatccggcc
    44161 tctttaatca cccatggctt cggtgtccat cgtttcattt ttcggcggat atccaataaa
    44221 gccattgaat ttattcaagt ttaggtagaa atcgccatga aacgcacttt tcaaccgtct
    44281 gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta tggctactaa aaatggtcgt
    44341 caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa
    44401 agctaacccc tgagtggtta agctcgcatt tcccagggag ttacgcttgt taactcccag
    44461 tcaattcaca ttcgtcttcc agcagccaca acgggctggc acgccgcaaa ttaccattct
    44521 cggccgcctg aattcgctgg ggcatccccg tatcggtctt acagtcgcca agaaaaacgt
    44581 tcgacgcgcc catgaacgca atcggattaa acgcctgacg cgtgaaagct tccgtctgcg
    44641 ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct
    44701 cgataaccgt gctctctcgg aagcgttgga aaaattatgg cgccgccact gtcgcctggc
    44761 tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc tgattagtcc gctactcggg
    44821 ccgcattgtc gtttcactcc aacctgttca agctacggaa ttgaggcatt gcgcaggttt
    44881 ggagtgataa aaggcagttg gttgacggtg aaacgcgtat taaaatgcca ccctttacac
    44941 cctggtggtg acgatcccgt cccgcccgga ccatttgata ccagagaaca ctaacgatgg
    45001 attcgcaacg caatctttta gtcatcgctt tgctgttcgt gtctttcatg atctggcaag
    45061 cctgggagca ggataaaaac ccgcaacctc aggcccaaca gaccacgcag acaacgacca
    45121 ccgcagcggg tagcgccgcc gaccagggcg taccggccag tggccagggg aaactgatct
    45181 cggttaagac cgacgtgctt gatctgacca tcaacacccg tggtggtgat gttgagcaag
    45241 ctctgctgcc tgcttacccg aaagagctga actctaccca gccgttccag ctgttggaaa
    45301 cttcaccgca gtttatttat caggcacaga gcggtctgac cggtcgtgat ggcccggata
    45361 acccggctaa cggcccgcgt ccgctgtata acgttgaaaa agacgcttat gtgctggctg
    45421 aaggtcaaaa cgaactgcag gtgccgatga cgtataccga cgcggcaggc aacacgttta
    45481 ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga
    45541 acgctggcga gaaaccgctg gaaatctcct cgtttggtca gttgaagcaa tccatcactc
    45601 tgccaccgca tctcgatacc ggaagcagca acttcgcact gcacaccttc cgtggcgcgg
    45661 cgtactccac gcctgacgag aagtatgaga aatacaagtt cgataccatt gccgataacg
    45721 aaaacctgaa catctcttcg aaaggtggtt gggtggcgat gctgcaacag tatttcgcga
    45781 cggcgtggat cccgcataac gacggtacca acaacttcta taccgctaat ctgggtaacg
    45841 gcatcgtcgc tatcggctat aaatctcagc cggtactggt tcagcctggt cagactggcg
    45901 cgatgaacag caccctgtgg gttggcccgg aaatccagga caaaatggca gctgttgctc
    45961 cgcacctgga tctgaccgtt gattacggtt ggttgtggtt catctctcag ccgctgttca
    46021 aactgctgaa atggatccat agctttgtgg gtaactgggg cttctccatt atcatcatca
    46081 cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc tccatggcga
    46141 agatgcgtat gctgcagccg aagattcagg caatgcgtga gcgtctgggc gatgacaaac
    46201 agcgtatcag tcaggaaatg atggcgctgt acaaagctga gaaggttaac ccgctgggcg
    46261 gctgcttccc gctgctgatc cagatgccaa tcttcctggc gttgtactac atgctgatgg
    46321 gttccgttga actgcgtcag gcaccgtttg cactgtggat ccacgacctg tcggcacagg
    46381 acccgtacta catcctgccg atcctgatgg gcgtaacgat gttcttcatt cagaagatgt
    46441 cgccgaccac tgtgaccgac ccgatgcagc agaagatcat gacctttatg ccggtcatct
    46501 tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtact gtactatatc gtcagcaacc
    46561 tggtaaccat tattcagcag cagctgattt accgtggtct ggaaaaacgt ggcctgcata
    46621 gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct aaaataaggg cggtcagttg
    46681 accgcctttt ttcttttcgt agggcggata agcaccgcgc atccgccaca caaagcaaca
    46741 ggaacatcat gagcgataat gacactatcg tagcccaggc cacgcctccg ggacgtggcg
    46801 gcgttggcat cctgcgcatc tccggcttca aagcccgtga agttgccgaa accgtgctgg
    46861 gtaaactgcc taagccgcgc tacgccgatt atcttccgtt taaagacgcc gacggcagcg
    46921 tgctcgatca ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg
    46981 tgctggaact gcaaggtcat ggcggtccgg tgatcctcga cctgctgtta aaacgcattc
    47041 tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta
    47101 acgataaact tgacttagcc caggccgagg cgattgccga tcttatcgac gccagttcgg
    47161 aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc attctccgca cgggttaatc
    47221 atctggtaga agccctcacc cacttgcgca tttacgtcga agcggcaatt gatttccctg
    47281 atgaagagat cgatttcctc tccgacggca aaattgaagc ccagcttaat gacgtgatgg
    47341 ccgatctcga tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc gaagggatga
    47401 aagtagtgat tgccggacgt cctaatgccg gtaaatcgag cctgttaaac gcgctggcgg
    47461 gccgtgaagc ggcaatcgta accgatatcg ccggaaccac gcgtgacgtg ctgcgtgagc
    47521 atatccacat tgacggaatg ccgctgcata tcatcgacac tgccgggcta cgtgaggcca
    47581 gtgacgaagt ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc
    47641 gcgtgctgtt tatggtcgat ggcaccacga cagacgccgt tgatccggca gagatctggc
    47701 cggaatttat cgcccgtctg cccgcgaaac tgccgatcac cgtggtacgc aataaagccg
    47761 atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct
    47821 cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca tctcaaacag agcatgggct
    47881 ttgacaccaa tatggaaggc ggcttcctgg cgcgccgtcg ccacttacag gcgctggaac
    47941 aggcagcgga gcatttgcag caaggtaaag cgcaactgtt gggagcctgg gcaggtgaac
    48001 tgctggcgga agagttgcgc ctggcacagc agaacttaag cgaaatcacc ggggaattta
    48061 cttcagacga cctgctgggg cggattttct ccagcttctg tattggtaag taaccgcgct
    48121 tac
//
LOCUS       DAJTOW010000019        47956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTOW010000019 DAJTOW010000000
VERSION     DAJTOW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.019"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO244_001190"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO244_001190"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5663095.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO244_001191"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO244_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5663096.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO244_001192"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO244_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5663097.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO244_001193"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO244_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5663098.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPDAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO244_001194"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO244_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5663099.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO244_001195"
     CDS             5377..5835
                     /locus_tag="OO244_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5663100.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO244_001196"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO244_001196"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5663101.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO244_001197"
     tRNA            6887..6971
                     /locus_tag="OO244_001197"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO244_001198"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO244_001198"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO244_001199"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO244_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5663102.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO244_001200"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO244_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5663103.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO244_001201"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO244_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5663104.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO244_001202"
     CDS             complement(10252..13995)
                     /locus_tag="OO244_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5663105.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO244_001203"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO244_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5663106.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO244_001204"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO244_001204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5663107.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO244_001205"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO244_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5663108.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO244_001206"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO244_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5663109.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO244_001207"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO244_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5663110.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO244_001208"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO244_001208"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5663111.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO244_001209"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO244_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5663112.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO244_001210"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO244_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5663113.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFAAQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO244_001211"
     CDS             23392..23499
                     /locus_tag="OO244_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5663114.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO244_001212"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO244_001212"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5663115.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO244_001213"
     CDS             complement(24538..25347)
                     /locus_tag="OO244_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5663116.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO244_001214"
     CDS             complement(25351..25743)
                     /locus_tag="OO244_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5663117.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO244_001215"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO244_001215"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5663118.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO244_001216"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO244_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5663119.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO244_001217"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO244_001217"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5663120.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO244_001218"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO244_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5663121.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO244_001219"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO244_001219"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5663122.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO244_001220"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO244_001220"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5663123.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO244_001221"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO244_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5663124.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO244_001222"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO244_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5663125.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO244_001223"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO244_001223"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5663126.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO244_001224"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO244_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5663127.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO244_001225"
     CDS             36790..39657
                     /locus_tag="OO244_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5663128.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO244_001226"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO244_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5663129.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO244_001227"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO244_001227"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5663130.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO244_001228"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO244_001228"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5663131.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDHEIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASDPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO244_001229"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO244_001229"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5663132.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO244_001230"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO244_001230"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5663133.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO244_001231"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO244_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12041 a        12345 c        11742 g        11828 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcgtcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgccg cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtagccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcacgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtgatccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttact cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaagtcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAJTOW010000020        47464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTOW010000020 DAJTOW010000000
VERSION     DAJTOW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.020"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            90..572
                     /gene="sseA"
                     /locus_tag="OO244_001232"
                     /pseudo
     CDS             90..572
                     /gene="sseA"
                     /locus_tag="OO244_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO244_001233"
     CDS             complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO244_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5663134.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO244_001234"
     CDS             complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO244_001234"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5663135.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO244_001235"
     CDS             complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO244_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5663136.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO244_001236"
     CDS             complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO244_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5663137.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO244_001237"
     CDS             complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO244_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5663138.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO244_001238"
     CDS             complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO244_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5663139.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO244_001239"
     CDS             complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO244_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5663140.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO244_001240"
     CDS             complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO244_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5663141.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO244_001241"
     CDS             complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO244_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5663142.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO244_001242"
     CDS             complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO244_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5663143.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO244_001243"
     CDS             complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO244_001243"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5663144.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10375..11178
                     /gene="suhB"
                     /locus_tag="OO244_001244"
     CDS             10375..11178
                     /gene="suhB"
                     /locus_tag="OO244_001244"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5663145.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11323..12177
                     /locus_tag="OO244_001245"
     CDS             11323..12177
                     /locus_tag="OO244_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5663146.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12368..13648
                     /gene="csiE"
                     /locus_tag="OO244_001246"
     CDS             12368..13648
                     /gene="csiE"
                     /locus_tag="OO244_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5663147.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13640..14779)
                     /locus_tag="OO244_001247"
     CDS             complement(13640..14779)
                     /locus_tag="OO244_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5663148.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO244_001248"
     CDS             complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO244_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5663149.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     TLALRDFIAIVQERLASVTA"
     gene            15965..17326
                     /locus_tag="OO244_001249"
     CDS             15965..17326
                     /locus_tag="OO244_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5663150.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17323..17841
                     /locus_tag="OO244_001250"
     CDS             17323..17841
                     /locus_tag="OO244_001250"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5663151.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17841..18161
                     /locus_tag="OO244_001251"
     CDS             17841..18161
                     /locus_tag="OO244_001251"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5663152.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18158..18970
                     /gene="hcaB"
                     /locus_tag="OO244_001252"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18158..18970
                     /gene="hcaB"
                     /locus_tag="OO244_001252"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5663153.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18980..20196
                     /locus_tag="OO244_001253"
                     /pseudo
     CDS             18980..20196
                     /locus_tag="OO244_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20293..20715
                     /locus_tag="OO244_001254"
     CDS             20293..20715
                     /locus_tag="OO244_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5663154.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YIASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20763..21635)
                     /locus_tag="OO244_001255"
     CDS             complement(20763..21635)
                     /locus_tag="OO244_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5663155.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21647..22741)
                     /locus_tag="OO244_001256"
     CDS             complement(21647..22741)
                     /locus_tag="OO244_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5663156.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22774..23772)
                     /locus_tag="OO244_001257"
     CDS             complement(22774..23772)
                     /locus_tag="OO244_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5663157.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23797..25308)
                     /locus_tag="OO244_001258"
     CDS             complement(23797..25308)
                     /locus_tag="OO244_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5663158.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25331..26313)
                     /locus_tag="OO244_001259"
                     /pseudo
     CDS             complement(25331..26313)
                     /locus_tag="OO244_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26410..29540)
                     /locus_tag="OO244_001260"
                     /pseudo
     CDS             complement(26410..29540)
                     /locus_tag="OO244_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            complement(29547..29720)
                     /locus_tag="OO244_001261"
     CDS             complement(29547..29720)
                     /locus_tag="OO244_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663159.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29838..31031
                     /locus_tag="OO244_001262"
     CDS             29838..31031
                     /locus_tag="OO244_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5663160.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31229..32482)
                     /gene="glyA"
                     /locus_tag="OO244_001263"
     CDS             complement(31229..32482)
                     /gene="glyA"
                     /locus_tag="OO244_001263"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5663161.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32809..33999
                     /gene="hmpA"
                     /locus_tag="OO244_001264"
     CDS             32809..33999
                     /gene="hmpA"
                     /locus_tag="OO244_001264"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5663162.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34044..34382)
                     /gene="glnB"
                     /locus_tag="OO244_001265"
     CDS             complement(34044..34382)
                     /gene="glnB"
                     /locus_tag="OO244_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5663163.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34443..35777)
                     /gene="glrR"
                     /locus_tag="OO244_001266"
     CDS             complement(34443..35777)
                     /gene="glrR"
                     /locus_tag="OO244_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5663164.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35767..36480)
                     /gene="qseG"
                     /locus_tag="OO244_001267"
     CDS             complement(35767..36480)
                     /gene="qseG"
                     /locus_tag="OO244_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5663165.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36645..38072)
                     /gene="qseE"
                     /locus_tag="OO244_001268"
     CDS             complement(36645..38072)
                     /gene="qseE"
                     /locus_tag="OO244_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5663166.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38630..42517)
                     /gene="purL"
                     /locus_tag="OO244_001269"
                     /gene_synonym="purI"
     CDS             complement(38630..42517)
                     /gene="purL"
                     /locus_tag="OO244_001269"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5663167.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42777..44333
                     /gene="mltF"
                     /locus_tag="OO244_001270"
     CDS             42777..44333
                     /gene="mltF"
                     /locus_tag="OO244_001270"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5663168.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44330..44866)
                     /gene="tadA"
                     /locus_tag="OO244_001271"
     CDS             complement(44330..44866)
                     /gene="tadA"
                     /locus_tag="OO244_001271"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5663169.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44891..45526)
                     /gene="yfhb"
                     /locus_tag="OO244_001272"
     CDS             complement(44891..45526)
                     /gene="yfhb"
                     /locus_tag="OO244_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5663170.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45735..46583
                     /locus_tag="OO244_001273"
     CDS             45735..46583
                     /locus_tag="OO244_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5663171.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46922..47464)
                     /locus_tag="OO244_001274"
                     /pseudo
     CDS             complement(46922..47464)
                     /locus_tag="OO244_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11315 a        13000 c        11949 g        11200 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg gacttgcagg ctggcagcgc gatgacctgc
      121 tattagaaga aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg
      181 aagcagtggt gaaagtaacc gatgtgttat tggcaagcca tgaaaatacg gcgcaaatta
      241 ttgatgcccg cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac
      301 gtcgcggaca tattcccggt gcattgaacg ttccgtggac ggaattggtg cgcgaaggcg
      361 aactaaaaac gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctatgaca
      421 aaccgattat cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg
      481 cgacgctgga tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc
      541 gggcagattt accggttgag ccagtgaaat aagtatatta caggcaataa aaaaccgccg
      601 aatttggcgt ttttttattg ctggtctggt tcgcggcctt tccagcaggt tgacttgtgt
      661 tacatgagca acgcaggtgc ttcacagcaa aacaatactc accagtaact ctctttttgt
      721 caagcaaaag agagtaatta ttgtttattt agcgtattat cgacaccggc cctttccgcc
      781 gtgttcggta ataaaataac ctggcttatt agtccgaatt cagacaaata taaacaaatc
      841 ctgctcaaaa ttaaaaattc taaccggtaa aagatattac ttaaacatgt aaatttactt
      901 tcctttaaaa aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg tccggttcgc
      961 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
     1021 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
     1081 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
     1141 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
     1201 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
     1261 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
     1321 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
     1381 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
     1441 gttcatagaa tggcgcaata tgttcggtaa taaagtggct aattcctttt tccccttttt
     1501 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
     1561 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
     1621 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
     1681 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
     1741 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
     1801 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
     1861 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
     1921 gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
     1981 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
     2041 cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg
     2101 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
     2161 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
     2221 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
     2281 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
     2341 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
     2401 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
     2461 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
     2521 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
     2581 cttgtgaggc actcgccagt aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
     2641 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
     2701 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
     2761 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
     2821 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
     2881 tcagtccgcg cataatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
     2941 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
     3001 tgataccttt acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt
     3061 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca
     3121 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
     3181 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
     3241 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
     3301 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc gggcctttat
     3361 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
     3421 gcttgatgcc cagaccgtca atcttgcggg ccgcacgctg gatcagcccc agatcgtctg
     3481 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
     3541 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
     3601 tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct
     3661 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
     3721 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
     3781 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac
     3841 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
     3901 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
     3961 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt
     4021 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
     4081 gttcaatctc aataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt
     4141 ccagaacagc gccatcaggg cagaggtcct gatgaggcaa aataacaatc tttggcatat
     4201 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
     4261 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca
     4321 tcatcgccct gcgccacttc actcaggtga gcggcagcat tgtcaatgac ctgacgctct
     4381 gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc
     4441 gccgcttcta ctttttgttc tgccagcata cgggctttta cgtcctgctc ggcatagctc
     4501 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
     4561 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
     4621 gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca
     4681 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
     4741 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
     4801 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
     4861 cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc
     4921 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
     4981 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
     5041 ccgcccacca tcaccacttc cagcacttcg tcagcttcta cacccgcgtc tttcagcgcg
     5101 cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
     5161 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
     5221 gcgattttgg ctgcaatggt ggcatccagc agttcacgct gaacgcggtt atcgctacga
     5281 tcaggaatgt ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
     5341 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
     5401 atggaaatat caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg
     5461 gaatccagcc cgtaggcgat agccgcagca gtcggttcgt taagtaagcg cagaacatgc
     5521 aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc
     5581 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
     5641 agtgctttga ggatgtccgc agaaacgcgc accgggttca gtagccccgc cgccgtttca
     5701 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
     5761 tggatatcag ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg
     5821 tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
     5881 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
     5941 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc
     6001 tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
     6061 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc
     6121 gagaaaacgc agcttacgca ccgtatccgc cgccgcgtcc cacgcctcgt tgtctaactg
     6181 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
     6241 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
     6301 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg
     6361 taaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
     6421 aatggttgca gattgctgta cggcggcgag ttgttccgcc aggcttccgc tggcgaattt
     6481 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct gggtatcgag
     6541 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
     6601 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
     6661 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
     6721 ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta
     6781 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
     6841 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
     6901 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
     6961 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
     7021 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
     7081 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
     7141 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
     7201 agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
     7261 gttgacttta atctgcaact tcattacgtc gccacaggcc ggtgcaccca ccatgccgct
     7321 gccgacgttc tcgtcgttgt tatcaaagga acccacgtta cgcggattct cgtaatggtc
     7381 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
     7441 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
     7501 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
     7561 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
     7621 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga
     7681 aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
     7741 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
     7801 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
     7861 ctcttctttt gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt
     7921 gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg
     7981 cggtttacga cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga
     8041 gaaagacatc aggtcaactt tcaactggct caggtcgatt ggcagtttgc ccacgctctg
     8101 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat
     8161 atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc
     8221 acgcatcgcc gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta
     8281 ggtgacttcg aaaccttcgc gctccagctg acggcaagta tccagtaccg ctttgtgttc
     8341 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
     8401 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
     8461 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
     8521 acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga actgcatcat
     8581 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
     8641 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga
     8701 tgtacgaccg tgtttacgga gtctttagca ctccggcctg attctgaatt ctttttatta
     8761 agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg
     8821 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
     8881 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
     8941 atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac
     9001 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
     9061 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
     9121 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
     9181 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
     9241 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
     9301 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
     9361 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
     9421 gttgtcggat gcggtgtgaa caccttatcc gacctacgat tctgtacatt gtaggcctga
     9481 taagacgcgt taagcgtcgc atcaggcaac cactgttatt cggccttgtt acctttattc
     9541 tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc
     9601 gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata
     9661 aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc
     9721 agcggatacg gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag
     9781 gccatacgca cttcataggc aataacctga accgccattg ccaggttcag cgaactgtat
     9841 tccgggtttg ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag
     9901 ccgacgcgct cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt
     9961 ttcagaccgc attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg
    10021 gtgcccacca ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca
    10081 ttaccaatca catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc
    10141 agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa
    10201 cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc
    10261 tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc
    10321 gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat
    10381 ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac
    10441 tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac
    10501 gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac
    10561 accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt
    10621 atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct
    10681 atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac
    10741 gaactgttca ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt
    10801 agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa
    10861 cagtacgcca ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc
    10921 cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt
    10981 ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa
    11041 gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc
    11101 gttgctggta acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc
    11161 gacgctctga agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca
    11221 ggcgctattc cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct
    11281 ttccccgatt aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac
    11341 aaacgcgttc ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg
    11401 ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc
    11461 tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt
    11521 attccttctt cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc
    11581 aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc
    11641 aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc
    11701 ggattgctgg acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac
    11761 ccacaacagc tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt
    11821 ggtcagggtg atcgtgaagg catacgtgcg gtaatcctcg actccacatt tgcctcttat
    11881 gcaaccatcg ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc
    11941 ggcgaaaatt acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc
    12001 gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa
    12061 cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat
    12121 gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc
    12181 atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc
    12241 agaacagttt aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat
    12301 tcccttcgcc atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga
    12361 tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg
    12421 ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga
    12481 gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat
    12541 cctgcgctat catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg
    12601 tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg
    12661 tccgtcattt attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg
    12721 aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg
    12781 acggctgcaa aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata
    12841 ttgtttgctg cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg
    12901 ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg
    12961 ggcgctccag catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt
    13021 gcgggttccc gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat
    13081 caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg
    13141 gttatgtgat cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat
    13201 cggtattgat aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac
    13261 cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga
    13321 aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga
    13381 aaaacagatt attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca
    13441 gttacgcgaa ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt
    13501 gcagacaggc gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc
    13561 gctcttttca ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca
    13621 gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg
    13681 gaagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac
    13741 cggcgaaaac ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct
    13801 gtaaacggat gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc
    13861 agaccgtgaa ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag
    13921 ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc
    13981 gagcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca
    14041 cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa
    14101 tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa
    14161 agcgccagtt ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc
    14221 ggcttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca
    14281 cgcccaacgt caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg
    14341 tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa
    14401 gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga
    14461 aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga
    14521 aggcgacagc aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg
    14581 aaggatcgct gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg
    14641 cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc
    14701 agaaaggtaa aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc
    14761 gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga
    14821 atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc
    14881 agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt
    14941 atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagcg
    15001 tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg
    15061 gacgaaaaac aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc
    15121 ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat
    15181 ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat
    15241 acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt
    15301 catgtgctaa agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca
    15361 gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac
    15421 ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg
    15481 gctgtctgag acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca
    15541 caaagccaat ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat
    15601 tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca
    15661 acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc
    15721 ggatctggct gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt
    15781 tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct
    15841 tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac
    15901 cagcaacatc cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa
    15961 aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg
    16021 ggtcactccg cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt
    16081 cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt
    16141 taacacctac atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa
    16201 ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa
    16261 cagtcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat
    16321 cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct
    16381 aaacgaagtt ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag
    16441 cgcaccgggc ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga
    16501 tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa
    16561 ctggaaattc ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc
    16621 ttctgccgtt caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg
    16681 acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg
    16741 tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc
    16801 agtttcaagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg
    16861 cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac
    16921 tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg
    16981 tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg
    17041 tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca
    17101 gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg
    17161 tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac
    17221 tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga
    17281 agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca
    17341 ctggagttac atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac
    17401 tggaaatttc gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc
    17461 acggttaacg cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt
    17521 ttcaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc
    17581 tgggcagaag agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa
    17641 accgacatcc caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa
    17701 gagcgcgatg aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat
    17761 gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat
    17821 aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg
    17881 agggtgaggc tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg
    17941 agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc
    18001 tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg
    18061 ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag
    18121 gtggtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc
    18181 catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga
    18241 agaaggcgcg caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca
    18301 gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca
    18361 acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc
    18421 aggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg
    18481 cttccacgag ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc
    18541 tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta
    18601 tcctggcggc ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg
    18661 ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat
    18721 ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc
    18781 tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga
    18841 ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt
    18901 gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct
    18961 ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc
    19021 ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt
    19081 ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga
    19141 agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca
    19201 tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac
    19261 caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc
    19321 gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc
    19381 cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac
    19441 tattggtctg gaactggctg ccagcgccac gcagcgcagc gcagcgcagc gcagcgcaga
    19501 tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc accaccgccc
    19561 gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct gctcaataat
    19621 gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag tggggaaacg
    19681 cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca actggctcgc
    19741 gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg cacctgcgat
    19801 cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg tgcactacac
    19861 cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc cgcaatgtta
    19921 gggctaccgc tactgccgcc gccgtggttc tggagcgatc agtacagtga taacttacag
    19981 tttattggcg atatgcgtgg cgatgactgg ctttgtcgtg gcaacccgga aactcagaag
    20041 gcgatttggt ttaatctgca aaacggcgtg cttatcggtg cggtcacgct gaatcagggc
    20101 cgtgagattc gcccaattcg caaatggatc cagagcggca aaacgtttga tgcgaaactg
    20161 ctgatagatg agaacatcgc gcttaaatca ctgtaatcag gataattagc gaatatctca
    20221 atgcctgggg cgtggcgagg tgcaagagtg tgtattacgt ttaaatcaca ttatcttgca
    20281 aagggattgg ttatgaacac actacgttat tttgattttg gagctgcccg ccccgttttg
    20341 ttattaattg cccgtatcgc cgtggtctta attttcatta tttttggttt tcccaaaatg
    20401 atgggctttg acggtacggt ccaatatata gcctcgttgg gcgcgccaat gccgatgctg
    20461 gcagcgatta ttgcggtagt tatggaagtg cccgccgcga tattaatcgt gcttggcttt
    20521 ttcacccgtc cgctggcggt gctgtttatt ttctacacgc tgggtacggc ggtgattggt
    20581 caccattact gggatatgac cggcgatgcg gttgggccaa atatgattaa tttctggaag
    20641 aatgtcagta tcgctggcgc gttcttgcta ttggcaatta ccggaccggg ggcaatttct
    20701 ctcgatcggc gttaggaaag atgccggatg cggcgtgaac gccttatccg gcattttata
    20761 aattacagcc actctacccg caacgacatc tctgaggttg ttgattcccc tggtgctagc
    20821 gcaatgagat cacccccttc cgggcgatga tgctcatccg gcgcatggct catcggttcc
    20881 agacagaaga aatcaaacgc atatccttta tcaaacgcag ggtcggaaac aaagatgaaa
    20941 taacacggtg caggtggcgt cgtttccatg atgatggcat aaccttcctg cggctgctcg
    21001 atgcgggcct gaccattcca tccggcaaaa ccattgttca cccactgacg cggcaacggc
    21061 gcgagctggt taaaatccag ttcctgcggt agctgctcgc aaaactcacc cgccagccac
    21121 tgctcccgtt ccagccagta accgctcgcc tgcgcctgaa tccgcgtttg tggtgacaac
    21181 ggaaaataag gatgccagcc ggtaccaaat ggcagcgtct ctgccccttg attggtgaca
    21241 gagagcgtca ccgtcagcgt atccgccgtt aaatgaaacg cctgacttac ccgatagtga
    21301 tagacaccgc tgcgatgttc atacaccaga cacaaactat catcgctgtg cgagacacat
    21361 tgccactcgc ccagccagcc atcgccgtgt agatagtgcg catcccactc aacattcggt
    21421 tgcagttgat actcacgccc ctgccagaca aaacggttgc cgctcacccg gtttgcaaat
    21481 ggcaccagcg gaaaacatga ggcatcggtt gccacaccgc tttttttacc aggacgtaag
    21541 agcggcgtcg tatcgcgcca gaagccttcg attacgccgc cctggtcaga aacgtccagc
    21601 tttagcgacc cgtgggataa ggtatagatg gtcatccgct aactccttaa tccgggaagt
    21661 taatcacaac tttcccgcat ttgccgctcg ccatcagcac ataggcatca cctgcctgtt
    21721 ctagcgagaa gcgatgggta atggcgttac gcggccacag cttccagtca gtcaaatcgt
    21781 gggcgcattt ttccatatgg aacagactgg tcacccagga gccgataatc cgccgttgat
    21841 ggtgcatcag atcagcgctg acctcgaatt ccacttttcc ggtttcacca atgtaaacca
    21901 cccgccccca gtcagcagtg gattgcagtg ccagcaagcg acctgcggca ttaccggaac
    21961 aatcgagcgc aacatccgcg ccaccgtggg taagttcagc aattatctgc ggcagacctt
    22021 cggtggttgc taaatagccg tgatccatcc cccctaactg ttttgccatc gccagacgtt
    22081 ccggcagcat atcaacgccg atgatccgtt ttgcaccgcg acctttcgcc agcatcatcg
    22141 ccatcatgcc gaccggcccc agaccgacca ccagcacgtt atcgctgccg gaaacttcgc
    22201 cgcgcaaaat cccttcatag gccgtgccaa cgccgcaact gataaacgca ccatcttcgt
    22261 agctcagcgc atccggcagg aggatcagat ctttttcttc cgccagcaga tattcggcat
    22321 gaccgccgtc acgctgccag ccgtaagccg cttttccttt gccagtacaa gagataggaa
    22381 agccgcggcg gcagttcggg caaaaaccac agccagaaat gtgatacacc agcacgcggt
    22441 cgccctcttt aaaatggcgg cagccttgcc ccatcgccac aatctgcccg cacggttcat
    22501 gaccgttgat aaatccctgg tataacggtt tatcgggtgc cgccgccgtg gcacggtgtt
    22561 gatgatagat atagtggaca tcgcttccgc aaatcccgga ggatttcatt ttgatcagta
    22621 cctggttaat ccccggcgtc ggcaccgcaa cttcccgcag atcgacggtt gaatttccta
    22681 gtaaataagc agccagcatc gttttcataa atccctcatt aacaatacga ttaattttca
    22741 tccctgcagc acccgcgcca ggggcgttaa ggtttagcgt ttcgctttac tgcttcgctg
    22801 ggtcagcaag atattcgcca gcaccgccac cacgatgatg acgccgcgta ccacctgctg
    22861 gaaaaaggag ttaataccga gcagcaccag accgttaccg attagcgtaa tcaccagcac
    22921 accaagcaat gtaccgaaca gggaaccgcg accgccggaa agtgccgtac cgccgaccac
    22981 gaccgcggcg atgacgtcaa actccagacc gtttgcggca cctgcgttac cggaaccgag
    23041 gcgtgccgcc aacaaaatgc cggtcaccgc cgctaataat cccgaaaggg taaagataag
    23101 aatgcgcact cgacgaacgt taataccgca caactgcgcc gccgtggcat taccgccaac
    23161 ggcaaaaacc gagcgcccga aggcggtttt tcggctaatg aacacaaaca gcgcaaacaa
    23221 caccatcatg atcagcgcgg atactggcac accgagaaat tgtccgccca gccagtccag
    23281 cacctcgttt tcgtcaatcg gcactggcag tgcgttggtc ataaacagcc ccattccgcg
    23341 cagagcgctc cacagcccca gtgtggcaac gaaacttggt acgttaaaca cgccgcgcag
    23401 caccccggcg agcgttccca tcagcgcgcc taacagcaac accagcagac aagcaatcgc
    23461 caacggaact tcaaattgca gcaaaaatgc caggcacacc gagacaaaag ccaccatcgg
    23521 cccaacgctg acatcaattt caccggagat aataatcagc gtcatcgccc aggcggcaat
    23581 cccaatggtg gcggcatcgc gcagcacgtt catctggtta ttcaatgaga taaagccagg
    23641 cgcgttcagg gagaagacca gataaagaat ggcaatcacc accagcaaac cgatctcatt
    23701 aatatggcga ctgacaaatt gtttgagcga gacgctcttg ccctgcggca atggtaatga
    23761 cgaagcatac atcttcaggt tcctcggtaa aattcatcag tgcacagaca gaatggcgga
    23821 catcagctca tccacattga ccggcgagtg aaactcctgc gagaacgtgc cgtgctgtaa
    23881 taacaggatg cggtcacaca ccagcggcaa ttcctccact tcactggaga taaacaccac
    23941 gctttttcct tcggcagcca gctcacggac aatacggtaa atctgctgtt tggcttcgat
    24001 atcgacgccg cgcgttggct cgtcgagcaa caaaatctgg ctggcggcat agacccaacg
    24061 accgatcacc actttttgct gattgccacc agaaagcgtg ccgatgggtg tttcgctact
    24121 ggcagccttg accgtcatcc gctgcatcac ctcttcggtc aggcggcgga tggtggacca
    24181 ttgcagcaca ccgttggtgc tgattttttg ccgattggtc agcactgtat tttcgtcaac
    24241 gcctagccag ggaatgatcc ccgcttcttt gcggttttct ggcgtatagc caatgccgcg
    24301 tttcagcatg tcgccgtaat cggggcgcat gattttctcg ccgttgataa caatttcgcc
    24361 ctgttcatac gtctccagcc caacaatcgc cttcagcaat tcactgcgcc ctgcccccag
    24421 caggccagca atgccaagca cttcgccacg acgtagcgta aagctgatat cctccagctt
    24481 gggcttatgg cgtaacgcac ggacttccag tacggcctga tccacaattt cctgaggggc
    24541 aaccggcgca atatcaacgt gatcacgccc tagcattagc gacacaatat gatgcgtgga
    24601 ggtgttttcg agcatcacat cgcccgccac ctgaccatcg cgcataacgg tggcatagga
    24661 ggcaatgcgg cggatttctt ccatccggtg gctgacataa atcaccgcca cgcccagtgc
    24721 cgacatcttt ttcaccgcac tgatcaccag ttcaacttct gcactggcaa gcgagctggt
    24781 gggttcatca agaatgacca cgcgcggctc gcccttcatc acccgtgcaa tttccaccag
    24841 ttgcttttgc gccgggctta gcgttgaaac aagttgttca ggactaacgt caacgcccag
    24901 cgcctgtaag caacgttggg catcctgcgc catttgcagg taatcaatca tgccgttgcg
    24961 gcgaggccat tgaccaaggc agaggttttc tgccaccgtc agcccttcca ccagacttaa
    25021 ttcctgataa accgcacgaa cccccagttc agcggcacgg cgagtcaacg tagcttcgtc
    25081 accttccagt cgcgtctcgc caatccagat atcaccgcta tccgggcgtt cgctgccggt
    25141 aagcattcga atgagagtcg atttgcccgc gccgttttta cctaacagcg cacgaacttc
    25201 gcctttattg agcgtgaagt taacgttatc cagcgcaacg acgccggggt aacgcttatt
    25261 tcctgccacc acttttgcta ccgggactgc ctctgttgcc gtgaacatag ggacctctgc
    25321 gaatcagcga ttagggcaga ccatcaacgt gcgttgccag ccactgttta ccatcttccg
    25381 ttttggtata gagatcgatt ggcacctgaa tcactttttc accgtcggct tgtttattga
    25441 taaccttcaa tgtttgcgcg aaaacagcat tgcccatttt cttaccggaa atatccacta
    25501 ccgctttcag cacctgattg ttttccagct cctgagcaat ttcggttgtc atatccgaac
    25561 cgaaaacagc aatttttccg gcctgatttt gattacgtac cgcttttacc gcgccgagtg
    25621 tcgcaccgcc cgactccccc ataatggcgt tgagatccgg cgtggagata atcagttttt
    25681 caccaacgga aatcgcttta tctaaaacag tcccttcctg gttagcgaca atttgcgcgc
    25741 cgggaacgcg ggattttaat acttcttcaa atcctttacg tcgctgcaca caaacttcaa
    25801 aggcttcgca attgatgacg gcaattttcg gctggtcaat tttattggca ataaaataat
    25861 cggcggcagc gttacccagt ttttaccaaa ttccagtgga tcgccgacca gatacgccga
    25921 gacatattta tcgacaccct tttgattaat acaggtgttg tagcaaatca ccggaatacc
    25981 cgcttcactg gcgcgacgaa cggtacggct actgccattt tcagacactg ccgataaaat
    26041 aatggcatcg acattacgcg ccacgagggt atcaacaaag gtactttctt tcgaaatatc
    26101 cccctgggcg ttagtttcaa ttaactgtac ctgtactgaa gaatcttttg ccgcatcctg
    26161 aacgccctga cgcactccag cgtaatatcc ctgggtatca aggtatattg caccaatggt
    26221 catttcttta tccgcagccc tggcaaatag tgcgctacct aatagcgtag ccattaataa
    26281 tagattacgg gttgttctca tttttttagg catagtgcct cctgtagggt ttttattaac
    26341 aacggcttat tctaattatt ttgtgatgag cggcagcgcg tgccgctcaa atattacagt
    26401 taatagggtt tagtgaacgt agttaaaaat aaaaggcatc acagtggtga ataatgccgc
    26461 tttcggccag gccggattaa tcgccgtcaa ttcagcgcga gcagactcat attgcgcaac
    26521 ctccccgagt cccgcggacg ccagcatcgc caccatcagg catttttctt tatgctgctg
    26581 ttgtaaatcg ccatacagcg ataacaggtc aggttgtgaa acagcaaaga aatccgcctc
    26641 aatactggtt ttcgccatct cttgcgccca ctgtttcatt tcactaaaca gttgctgtgc
    26701 ggtttgctgt tcacccagca gtcgcagcgc cattccttgc cagaagagat aatcaaccgg
    26761 ctgatcgttg taataactgt ggatgttaat ggtgcgatcg ccggtcgccg ccagacgtaa
    26821 acaacgcatc gcttcagttt catcgccctg cgcgttggcg catatcgcct gccagaacca
    26881 gatgtcgtta tcagtttgcc ccggtaaacg gccttcgctt aaattctccg gataatacag
    26941 cgcggcatga agcagttcgc tggcctgctg cggctctctg gcatcaagat gctgccaggc
    27001 gcgtaataac tggttgagga taaactgact ggtgaccttc ccttccccgc cttcccacgg
    27061 gtggaattta cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg tgagatgcca
    27121 caaattgagc agttctgcgg tcatgtcgtc gcgtttcagc gcaatttcca gattattttc
    27181 cagacgcgcc agtcgtttct ccggtgtggc tccacttaac ttatccagca aatcccgttc
    27241 gaaaagcaga cgtgcatcct gcggcgcaag ctgataagca ttatcaagat aacgcgcggc
    27301 cagctcataa tcgtgtttct tattccacgc atggatcgct aacccgcgcc agccgtcggc
    27361 aaactccggc gacatctcta cgcaacgttg ccataaggca atggctttgt tgtagctacg
    27421 tttgttgtag tagaagcagg ccagtaaatg gcgagcaaac cagcactctt caatactctc
    27481 cagcgccgcc acttcttcca gcgtattcgg gaaacggaca aactgcggga agacatcaat
    27541 ggcttttgcg accagttcgc cacgttcggc tttcggcagc aagctggctt gcaggtaaag
    27601 cggcagcgtg cgctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    27661 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    27721 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    27781 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    27841 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27901 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27961 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    28021 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    28081 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcgggtt tttgttcagc
    28141 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    28201 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    28261 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    28321 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    28381 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    28441 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    28501 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    28561 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    28621 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    28681 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    28741 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    28801 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    28861 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28921 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28981 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    29041 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    29101 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    29161 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    29221 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    29281 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    29341 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    29401 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    29461 actttatccc atgcgcgatg cccccgaccg cccagttccg gcaggatcat cactttgatg
    29521 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    29581 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    29641 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    29701 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    29761 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    29821 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    29881 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29941 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    30001 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    30061 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    30121 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    30181 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    30241 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    30301 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    30361 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30421 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30481 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30541 gcggacagat tggtcacacc atcgttaatc cggatggcgt cgtctgcgac tgtggacgtt
    30601 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30661 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30721 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30781 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30841 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30901 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30961 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    31021 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    31081 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31141 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31201 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31261 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31321 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31381 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31441 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31501 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31561 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31621 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31681 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31741 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31801 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31861 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31921 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31981 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    32041 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    32101 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32161 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32221 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32281 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32341 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32401 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32461 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32521 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32581 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32641 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32701 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32761 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32821 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32881 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32941 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    33001 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    33061 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33121 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33181 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33241 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33301 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33361 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33421 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33481 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33541 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33601 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33661 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33721 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33781 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33841 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33901 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33961 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    34021 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    34081 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34141 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34201 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34261 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34321 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34381 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34441 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34501 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    34561 agcttacgca aatagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34621 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34681 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34741 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34801 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34861 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34921 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34981 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    35041 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    35101 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35161 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35221 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35281 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35341 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35401 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35461 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35521 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35581 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35641 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35701 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35761 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35821 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35881 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35941 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    36001 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    36061 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36121 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36181 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36241 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36301 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36361 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36421 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36481 attcaccaga cttaaagcct accccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36541 acagcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36601 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36661 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36721 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36781 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    36841 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36901 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36961 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    37021 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    37081 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37141 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37201 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37261 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37321 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37381 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37441 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37501 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37561 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37621 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37681 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37741 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37801 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37861 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37921 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37981 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    38041 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    38101 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38161 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38221 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38281 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38341 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38401 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38461 cttattagat tacaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38521 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38581 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    38641 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    38701 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    38761 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    38821 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    38881 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38941 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    39001 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    39061 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    39121 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    39181 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    39241 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    39301 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    39361 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    39421 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    39481 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    39541 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    39601 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    39661 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    39721 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    39781 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    39841 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39901 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39961 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    40021 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    40081 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    40141 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    40201 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    40261 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    40321 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    40381 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    40441 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    40501 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    40561 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    40621 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    40681 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    40741 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    40801 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    40861 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40921 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40981 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    41041 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    41101 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    41161 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    41221 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    41281 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    41341 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    41401 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    41461 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    41521 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    41581 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    41641 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    41701 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    41761 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    41821 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    41881 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41941 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    42001 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    42061 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    42121 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    42181 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    42241 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    42301 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    42361 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    42421 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    42481 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    42541 cggtgtaccc aagggggggg gaaacgggcg tcattataaa gaatctgatg cgctgacgaa
    42601 accgtttgcg tggaaataaa atcaccatcg tgaattagca acgcgtgccg ccaatggctg
    42661 taataagttg ccatctggcg caggtttacg caaaatgccg ctcatttatg agtaaacctt
    42721 tcactattat tacgtttttt caagctggga cgcgcacgac acagagaatt aactaattga
    42781 aaaaattaaa gattaattat ctgttcatcg gcattctggc actgctgctc gcggtcgctc
    42841 tctggccatc cattccctgg tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc
    42901 ggggagagtt gcgtgtgagc accattcata ctcccctgac ttataacgaa atcaacggga
    42961 aaccttttgg cctggattac gaactggcga aacagtttgc cgattacctc ggcgtaaaac
    43021 tgaaagtgac cgtgcggcag aatatcagcc agctgtttga cgaccttgat aatggtaacg
    43081 ccgacctgct ggcggcagga ctggtctata acagtgagcg ggtaaaaaat tatcagcctg
    43141 gccctaccta ttattccgtg tcacaacaac tggtttataa agtgggtcag tatcgcccac
    43201 gtacgctggg caacctgacg gcggagcaac tcaccgttgc accgggtcat gtggtagtta
    43261 acgatctcca gaccctgaaa gaaacaaaat tcccggaatt aagctggaag gtagacgaca
    43321 aaaaaggctc tgcggaatta atggaagatg tcatcgaagg aaaactcgat tacaccattg
    43381 ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg
    43441 atatcaccga tgaacaaccg gtgacttggt ttagcccgtt agatggcgat aatacccttt
    43501 ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg
    43561 aagagaaata cctggggcat ggcgatgatt ttgattacgt cgatacgcgc acatttttac
    43621 gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa
    43681 ttgactggcg tttgctggcc gctattgctt atcaggaatc gcactgggat gcacaggcca
    43741 cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg caaagcctcg
    43801 gcattacgga tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg
    43861 atatgatgag taaagtgccg gaaagtgtgc cggagaacga acggatctgg tttgccctcg
    43921 ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca
    43981 aagggaatcc tgacagttgg gctgacgtaa aacagcgtct gcctttactt agccagaaac
    44041 cctattacag caagctgact tacggctacg ctcgtgggca tgaagcctac gcttatgtcg
    44101 aaaatattcg taagtatcag attagcctgg tgggttatct gcaagagaaa gagaagcagg
    44161 ctacagaagc ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct acggagttgg
    44221 gcaaagagaa atttcctttt ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc
    44281 attctccctc tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg
    44341 aggattgcgc ttttttctgc gctttaattt cctggcggcg catgcgaaag aagtcactga
    44401 gcaacgccgc gcactcatcc gccagtattc cttccgtaat ttccactcgg tgattcatac
    44461 ccggatgatg cagcacatcc attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac
    44521 caaagaccac gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt acacatggtt
    44581 caagcgtgac atacaacgtg gcgtcgatca gacgataatt ttgcatcacc agaccaccct
    44641 gccgcagggc catgatttct gcatgtgcgg tgggatcatg gcgaccaatc gggcggttcc
    44701 agccttcgcc gattacccga ttgttatgca ctaataccgc gccgaccggc acttcccgct
    44761 catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat ccaatattcg tggctaaatt
    44821 cgacttcaga caaaaagaaa actccggtta taaaagcgcg gcgcattata cacggacgct
    44881 atgctttact ctattccagt tgctggagtt caccgcgcgg ggttactcgc caacgatgct
    44941 gacagaaata aagcagcgga ttgtcctgat tactgtcgct atagccactg tacagccgca
    45001 gcggcgtgcc gattttgcgc tccagttgtg cgaccttttc atgtcccaga caacgcatcg
    45061 tcaataccca accaccatag ccacgctgaa tttggctggc gataagatta acccgcggca
    45121 gccagggcgt atcgaaataa accgcttcaa ccagcggctg cggagagccg gtaatcaacc
    45181 agatatcagc atcggaactt aacaggtagg tggttaatcg ctcctgaacc agcggaaagg
    45241 cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg caacgtctgt aaacgtgctt
    45301 cgctgtgacc aaaagtgcac ccccacagaa gcagactcat cggccagcgt gccgcacgac
    45361 cttttatcaa taacgcaatg gctataatcg gtaacaacgg caggacaagt aacgcattca
    45421 gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa catatcctgc tgatgcaatg
    45481 ttccatctaa gtcaaaaaac accacacgac gctcgtgagt tgccaaacca tactcctctg
    45541 gactgaaaca tcctgattaa ttttcgctac tacatagcct aacagataga tcatcacttt
    45601 tccggcaaca atcgaccgca tcatggctaa ctggaatttt taattcatgg caattagcgg
    45661 caatggaata taaaattcac tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca
    45721 tcgcgaggaa agatatgaac ggcttacttc gtatccgtca gcgttaccaa gggcttgccc
    45781 agagtgataa aaaactggcg gattatctgc tgctacaacc tgatacggcg cgccatttaa
    45841 gctctcagca actggccaac gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc
    45901 aaaaactcgg ctataaaggt tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa
    45961 gccagccgga atcgccctcc gtgcccattc ataaccaaat ccgcggtgat gatccgttac
    46021 ggctggtcgg cgaaaaactg attaaagaaa ataccgccgc tatgtacgca acgctaaacg
    46081 ttaatagtga agagaaactg catgaatgcg taacaatgtt gcgctctgcg cggcggataa
    46141 ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga
    46201 agattggctt caatgctgcc gcactgcgcg atatgcatgc gctactcgca acagtacagg
    46261 cgtcgtcccc tgacgatctg ttattagcca tttcctacac cggtgtacga cgcgagttaa
    46321 acctggcggc agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt accggcttta
    46381 ctccgaatgc cctgcaacag cgtgcttctc attgcctgta taccattgcc gaagaacaag
    46441 cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg aatgttaacg gatttgctgt
    46501 tcattgcgct gattcagcag gatctggaac tggcaccaga acgtattcgt catagtgaag
    46561 cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt
    46621 tgttttgaga gtttccttat ggcgctgtta atcactaaaa aatgcatcaa ttgtgatatg
    46681 tgtgaaatca aataaaaaca catttatttt catagtgata cagcaatgtg ttattgaatg
    46741 tgttgttgga tttatggtgc aaaaaaattt tgcataagtt cagcaacaca aagaacacca
    46801 ctttctttaa cgtcctccgc gccctaaagg acggggcttt acggcgcaaa aggcctgtga
    46861 catacaacat taacttttca gtcaaccgta gcacgttctt gtatacgccg tgtctgcggt
    46921 tttatataac cctggctgga aacctcttat acaaagtcga ttcgccaaca tcatagatga
    46981 tcgccacctt ctggcgagaa actcctgatg caattagtcg cccggcctgc gcccattatt
    47041 ctggtgaaag tttgggacgg cgaccgccaa ttcgtccctg tgcgcgagca gcttccagtc
    47101 ctgcttttgt tcgttcaaca atcagttcac gttccatttc agccagggca cccatcacat
    47161 ggaagaaaaa gcgtcccatt ggtgtgctgg tatcaattga atcagtcaga ctacgaaagt
    47221 tgatgcctcg ttcgcgcaac gcctccacca gcacgacaag atgccgcata ctgcgcccca
    47281 gccgatccag cttccagaca accagtgtgt cgcctgccga taatgtcttg agcagttttt
    47341 tcagccccgg tctttcggac tttgtaccac ttatcttgtc ttcaaaaatc agctcgcatc
    47401 ctgcacagtt caacgcatta cgttgtagat cggtattctg gtcatttgtt gatacgcgta
    47461 cata
//
LOCUS       DAJTOW010000021        46475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTOW010000021 DAJTOW010000000
VERSION     DAJTOW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.021"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..380
                     /gene="fruK"
                     /locus_tag="OO244_001275"
     CDS             <1..380
                     /gene="fruK"
                     /locus_tag="OO244_001275"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="converts fructose-1-phosphate and ATP to
                     fructose-1,6-bisphosphate and ADP; highly specific for
                     fructose-1-phopshate; similar to PfkB; forms homodimers;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5663172.1"
                     /translation="RRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALW
                     VNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQS
                     NVGITDRPQLAAMMARVDLQPFN"
     gene            397..2088
                     /gene="fruA"
                     /locus_tag="OO244_001276"
     CDS             397..2088
                     /gene="fruA"
                     /locus_tag="OO244_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5663173.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            2512..3453
                     /locus_tag="OO244_001277"
     CDS             2512..3453
                     /locus_tag="OO244_001277"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5663174.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            3441..4379
                     /gene="psuG"
                     /locus_tag="OO244_001278"
     CDS             3441..4379
                     /gene="psuG"
                     /locus_tag="OO244_001278"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5663175.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            4473..5723
                     /locus_tag="OO244_001279"
     CDS             4473..5723
                     /locus_tag="OO244_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5663176.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(5794..6488)
                     /gene="yeiL"
                     /locus_tag="OO244_001280"
                     /pseudo
     CDS             complement(5794..6488)
                     /gene="yeiL"
                     /locus_tag="OO244_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            6619..7560
                     /gene="rihB"
                     /locus_tag="OO244_001281"
     CDS             6619..7560
                     /gene="rihB"
                     /locus_tag="OO244_001281"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5663177.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            7660..8910
                     /locus_tag="OO244_001282"
     CDS             7660..8910
                     /locus_tag="OO244_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5663178.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8966..10054)
                     /locus_tag="OO244_001283"
     CDS             complement(8966..10054)
                     /locus_tag="OO244_001283"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5663179.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(10057..10914)
                     /gene="nfo"
                     /locus_tag="OO244_001284"
     CDS             complement(10057..10914)
                     /gene="nfo"
                     /locus_tag="OO244_001284"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5663180.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(10988..12037)
                     /locus_tag="OO244_001285"
     CDS             complement(10988..12037)
                     /locus_tag="OO244_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5663181.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            12136..13017
                     /locus_tag="OO244_001286"
     CDS             12136..13017
                     /locus_tag="OO244_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5663182.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            13223..14692
                     /gene="lysP"
                     /locus_tag="OO244_001287"
     CDS             13223..14692
                     /gene="lysP"
                     /locus_tag="OO244_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5663183.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            14984..16975
                     /gene="cirA"
                     /locus_tag="OO244_001288"
                     /pseudo
     CDS             14984..16975
                     /gene="cirA"
                     /locus_tag="OO244_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(17007..17842)
                     /gene="yeiG"
                     /locus_tag="OO244_001289"
                     /pseudo
     CDS             complement(17007..17842)
                     /gene="yeiG"
                     /locus_tag="OO244_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            18100..18768
                     /gene="folE"
                     /locus_tag="OO244_001290"
     CDS             18100..18768
                     /gene="folE"
                     /locus_tag="OO244_001290"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5663184.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            18785..19861
                     /locus_tag="OO244_001291"
     CDS             18785..19861
                     /locus_tag="OO244_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5663185.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            20003..21043
                     /gene="galS"
                     /locus_tag="OO244_001292"
     CDS             20003..21043
                     /gene="galS"
                     /locus_tag="OO244_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5663186.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSNGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            21323..22321
                     /gene="mglB"
                     /locus_tag="OO244_001293"
     CDS             21323..22321
                     /gene="mglB"
                     /locus_tag="OO244_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5663187.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            22382..23902
                     /gene="mglA"
                     /locus_tag="OO244_001294"
     CDS             22382..23902
                     /gene="mglA"
                     /locus_tag="OO244_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5663188.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            23918..24928
                     /gene="mglC"
                     /locus_tag="OO244_001295"
     CDS             23918..24928
                     /gene="mglC"
                     /locus_tag="OO244_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5663189.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(24964..26177)
                     /gene="preA"
                     /locus_tag="OO244_001296"
                     /pseudo
     CDS             complement(24964..26177)
                     /gene="preA"
                     /locus_tag="OO244_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(26171..27409)
                     /gene="preT"
                     /locus_tag="OO244_001297"
     CDS             complement(26171..27409)
                     /gene="preT"
                     /locus_tag="OO244_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5663190.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPAEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(27603..27842)
                     /locus_tag="OO244_001298"
     CDS             complement(27603..27842)
                     /locus_tag="OO244_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5663191.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(27845..28564)
                     /gene="sanA"
                     /locus_tag="OO244_001299"
     CDS             complement(27845..28564)
                     /gene="sanA"
                     /locus_tag="OO244_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5663192.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(28714..29598)
                     /gene="cdd"
                     /locus_tag="OO244_001300"
     CDS             complement(28714..29598)
                     /gene="cdd"
                     /locus_tag="OO244_001300"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5663193.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHSWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(29728..30423)
                     /locus_tag="OO244_001301"
     CDS             complement(29728..30423)
                     /locus_tag="OO244_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5663194.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(30420..30818)
                     /locus_tag="OO244_001302"
     CDS             complement(30420..30818)
                     /locus_tag="OO244_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5663195.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            31057..32004
                     /gene="dusC"
                     /locus_tag="OO244_001303"
     CDS             31057..32004
                     /gene="dusC"
                     /locus_tag="OO244_001303"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5663196.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(32374..32457)
                     /gene="yohP"
                     /locus_tag="OO244_001304"
     CDS             complement(32374..32457)
                     /gene="yohP"
                     /locus_tag="OO244_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5663197.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            32681..34117
                     /gene="mdtQ"
                     /locus_tag="OO244_001305"
                     /pseudo
     CDS             32681..34117
                     /gene="mdtQ"
                     /locus_tag="OO244_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            34170..34931
                     /locus_tag="OO244_001306"
                     /pseudo
     CDS             34170..34931
                     /locus_tag="OO244_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35048..35626)
                     /locus_tag="OO244_001307"
     CDS             complement(35048..35626)
                     /locus_tag="OO244_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5663198.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            35796..36383
                     /locus_tag="OO244_001308"
     CDS             35796..36383
                     /locus_tag="OO244_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5663199.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            36557..37489
                     /gene="pbpG"
                     /locus_tag="OO244_001309"
     CDS             36557..37489
                     /gene="pbpG"
                     /locus_tag="OO244_001309"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5663200.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(37527..39242)
                     /gene="dld"
                     /locus_tag="OO244_001310"
     CDS             complement(37527..39242)
                     /gene="dld"
                     /locus_tag="OO244_001310"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5663201.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            39438..41735
                     /gene="bglX"
                     /locus_tag="OO244_001311"
     CDS             39438..41735
                     /gene="bglX"
                     /locus_tag="OO244_001311"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5663202.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            41987..42895
                     /gene="osmF"
                     /locus_tag="OO244_001312"
     CDS             41987..42895
                     /gene="osmF"
                     /locus_tag="OO244_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5663203.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKG"
     gene            42911..44068
                     /gene="yehY"
                     /locus_tag="OO244_001313"
     CDS             42911..44068
                     /gene="yehY"
                     /locus_tag="OO244_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5663204.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            44061..44987
                     /gene="yehX"
                     /locus_tag="OO244_001314"
     CDS             44061..44987
                     /gene="yehX"
                     /locus_tag="OO244_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5663205.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            44992..45723
                     /gene="yehW"
                     /locus_tag="OO244_001315"
     CDS             44992..45723
                     /gene="yehW"
                     /locus_tag="OO244_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5663206.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(45704..45811)
                     /locus_tag="OO244_001316"
     CDS             complement(45704..45811)
                     /locus_tag="OO244_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5663207.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(45871..46327)
                     /locus_tag="OO244_001317"
                     /pseudo
     CDS             complement(45871..46327)
                     /locus_tag="OO244_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11137 a        11427 c        12473 g        11438 t
ORIGIN      
        1 accgccgcga gttggaaatc tgggcaggcc gtaaactgcc tgaaatgaaa gatgtgattg
       61 aagctgcaca tgcgctacgt gaacaaggta ttgcgcatgt tgttatttca ctgggtgccg
      121 aaggcgcgct ttgggttaat gcctccggcg aatggatcgc taaaccaccg tcagtcgatg
      181 tcgtaagcac cgttggcgca ggggattcta tggttggtgg cctgatttat ggcttgctga
      241 tgcgtgaatc cagtgaacac acactgcgtc tggcgacagc tgttgcagcc ctggcggtaa
      301 gtcaaagcaa tgtgggtatt accgatcgtc cgcagttggc cgcaatgatg gcgcgcgtcg
      361 acttacaacc ttttaactga cagcaggaga ggcataatga aaacgctgct gattattgac
      421 gctaatctcg gtcaggcacg cgcctatatg gcgaagaccc tgctgggcgc ggcggcgcga
      481 aaagcaaaac tggaaatcat cgacaatccg aacgacgcag aaatggcgat tgttctcggt
      541 gattccatcc cgaatgacag cgcgctgaac ggtaaaaatg tctggctggg tgatatttcc
      601 cgggcagttg cgcaccctga gctgttcctg agtgaagcca aaggccatgc gaaaccttac
      661 actgcgccgg tcactgcgac agcaccggtt gccgccagcg gtccgaaacg cgtagttgcg
      721 gtgactgctt gcccgactgg cgtagcacac acctttatgg cggctgaagc cattgaaacc
      781 gaagcgaaaa aacgtggctg gtgggtgaaa gttgaaaccc gtggttctgt tggcgcgggt
      841 aatgcaatca ctcccgagga agtagccgca gcggatctgg tgattgtggc ggcagatatc
      901 gaagtggatc tggcgaaatt tgctggtaaa ccgatgtatc gtacctctac cggtctggcg
      961 ctgaagaaaa cctcgcagga actggataaa gcggttgctg aagcaacgcc gtatgaaccg
     1021 gcgggcaaag ctcaaacggc gaccactgaa ggtaagaaag agagtgcagg cgcttatcgt
     1081 cacttgctaa cgggcgtctc ttacatgctg ccgatggtcg ttgctggtgg tctgtgtatc
     1141 gcgctttctt ttgcttttgg tatcgaagcg tttaaagagc cgggtacgtt ggcagcggcg
     1201 ctgatgcaga ttggtggtgg ttcagccttt gcgctgatgg tgccggtact ggcaggttat
     1261 attgccttct ccattgccga tcgtccgggc ctcacgccgg gtctgattgg cggtatgctg
     1321 gcggtcagca ccggttctgg cttcattggc ggtattattg cgggcttcct ggctggttac
     1381 attgcgaagt taatcagtac gcaactgaaa ctgccgcaga gtatggaggc gctgaaaccg
     1441 atcctgatca ttccgctaat ttccagtctg gtggtcggtc tggcgatgat ctacctgatc
     1501 ggtaaaccag ttgctggcat tctcgaaggg ctgactcact ggctgcagac catggggact
     1561 gcgaatgcgg ttctgctggg ggcgatcctc ggtggcatga tgtgtactga catgggcggt
     1621 ccggtaaaca aagcagcgta cgcattcggt gtgggtctgc tgagtactca aacctatggc
     1681 ccgatggcgg cgattatggc ggcaggtatg gtgccaccgc tggcaatggg cctggcaaca
     1741 atggtggcgc gtcgcaaatt cgacaaagcg cagcaggaag gtggcaaagc cgctctggta
     1801 ttgggactgt gcttcatttc ggaaggtgca attccgtttg ctgctcgtga tccgatgcgt
     1861 gtgctgccgt gctgtatcgt gggtggggcg ctgactggcg caatctcaat ggcgattggt
     1921 gcgaaactga tggcaccgca cggtggtctg tttgttctgc tgatccctgg cgctattacg
     1981 ccggtattgg gttacctggt agcaattatt gccggtacgc tggtggcggg tttggcctat
     2041 gccttcctga aacgtccgga agtggacgca gtagcgaaag cagcgtaata aaaggtgtgt
     2101 tacagggcag aaatttcctg ccctgtaaca tttgatgagc gcaaagagaa tgaccagctt
     2161 gtgaatgtaa ttttatgaat ttaaatggtt ttatctttca gtagaaactt caaacgttca
     2221 tcaaacacta aggttatttc ctcaagggca gattattttc tgcccttttt tagtgttcct
     2281 gttctgtgca tcccgtcaca aattcctcgc ttttgtaatt caggcattta atcgcatttt
     2341 gtgagcctgc tcaaatctgc ccattttgcc tctctggcgg taatttactt tacgtaaaac
     2401 atgcatacta tgagcacatg tttaaatatg aacacatgtt taaaccgtct ttgcagtatc
     2461 tatattcttg tggattgaca gggttggtaa ttttttacca gaggacgtgc tatgcgcgaa
     2521 aaggattatg tcgtaattat aggttcggcg aatattgatg tcgccggata ttcacatgaa
     2581 tcattaaatt atgcggattc aaatccaggt aaaataaaat ttacgcctgg tggagtaggg
     2641 cgcaatattg cacaaaacct ggcgttgctg ggtaacaaag cctggctact gagcgccgta
     2701 ggcagtgatt tttatggtca atcgctgcta acgcaaacca atcaatctgg tgtttatgtc
     2761 gataaatgcc tgattgtgcc gggagaaaat acgtcgagtt atttatcatt actcgataat
     2821 accggtgaaa tgctggttgc tataaatgac atgaatatta gcaacgctat tacagctgaa
     2881 tatctcgcac agcaccgtga atttattcag agggcaaagg tcattgtcgc ggactgtaat
     2941 atcagtgaag aggcactggc atggattctg gataatgccg ccaacgtacc cgtatttgtc
     3001 gatccggttt cagcgtggaa atgtgtcaaa gtgcgcgacc gtctaaatca gaaccacact
     3061 ctgaagccaa accgccttga agcggaaacc ctgagtggga ttgcgctgtc agggcgtgac
     3121 gatgtggcaa aagttgctgc ctggttccat caacatggcc tgaaccgact ggtattgagc
     3181 atgggcggcg acggcgttta ttacagcgat atcagcggtg aaaatggctg gtctgcgccg
     3241 atcaaaacca atgttattaa tgttaccgga gcgggcgatg ccatgatggc gggacttgct
     3301 tcgtgttggg tagacggaat gccgtttgcc gaatctgttc gtttcgcaca gggatgttcg
     3361 tcaatggcgc tctcctgtga atacaccaat aaccccgatt tatcgattgc caacgttata
     3421 tcgttagtgg agaacgcaga atgtctgaat taaaaatttc ccctgaatta ttacaaattt
     3481 ctccggaagt gcaggacgct ttaaaaaaca aaaaaccggt tgtggcgctg gaatcgacca
     3541 ttatttctca cgggatgccg ttcccacaaa atgcccagac cgcaattgaa gtagaagaaa
     3601 ctattcgtaa acagggcgca gtacctgcca ctatcgccat tattggcggc gtgatgaaag
     3661 tgggtttaag caaagaaaaa attgaattac tgggtcgtga agggcataac gtgaccaaag
     3721 ttagtcgtcg cgatttacct tttgttgttg ccgccggaaa aaatggcgca accactgtgg
     3781 cttcaacgat gattattgcg gcgcttgccg gaattaaagt atttgccacc gggggaattg
     3841 gtggtgtgca tcgcggggcg gaacatacct tcgatatttc tgccgatttg caagaactgg
     3901 caaatactaa tgtcaccgtt gtttgtgccg gggcgaaatc tattctcgat ttaggattaa
     3961 ccactgagta tttagaaacc ttcggtgtgc cgttaattgg ctatcagact aaagcgctgc
     4021 ctgcgttttt ctgccgtacc agctcgtttg acgtcagcat tcgtctcgac agcgccagtg
     4081 aaattgcccg tgcaatggcg gtgaaatggc aaagcgggct gaacggtggc ctcgtggtag
     4141 cgaacccgat cccggaacag tttgcgatgc cggaggaatc tatcaatgca gccatagatc
     4201 aagccgtcgc cgaagccgaa gagcagggcg ttattggtaa agaaagtaca ccgttcctgc
     4261 tggcgcgcgt tgctgagctg accagcggtg acagcctgaa atccaatatc cagctggtgt
     4321 tcaacaacgc cattctggcg agtgaaattg ccaaagaata ccagcgtctc gcgggttaat
     4381 ttctgacggg cagaaagccg tcgcccgtac tcatcaagca taccaaaacc tggcgctgac
     4441 gccgggttta ctggcatgaa gggaaaatca ttatggatat aatgagaagt gttgtgggga
     4501 tggtggtgtt actggcaata gcatttctgt tgtcagtgaa taaaaagagc atcagtttgc
     4561 gcacggttgg agccgcactg ctgctgcaaa tcgctattgg tggcatcatg ctctacttcc
     4621 caccgggaaa atgggcagta gaacaggcgg cattaggcgt tcataaagtg atgtcttaca
     4681 gtgatgccgg tagcgccttc atttttggtt cgctggttgg gccgaaaatg gatgtcctgt
     4741 ttgacggtgc gggttttatc ttcgcctttc gcgtacttcc ggcgattatt ttcgttactg
     4801 cgctcatcag tctgctgtac tacattggcg tgatggggct gctgattcgc atccttggca
     4861 gcattttcca gaaagccctc aacatcagca aaatcgaatc ttttgttgcg gttaccacta
     4921 ttttcctcgg gcaaaatgag atcccggcga tcgttaaacc gtttatcgat cgcgtgaatc
     4981 gcaacgagtt gtttaccgca atttgtagcg ggatggcgtc cattgctggt tcggtgatga
     5041 ttggttatgc cggaatgggc gtaccaattg actacctgtt agcggcatcg ctgatggcga
     5101 tccccggcgg tattttgttt gcacgtattc ttagcccggc aaccgagcct tcgcaggtca
     5161 catttgaaaa tctgtcgttc agcgaaacgc cgccaaaaag ctttatcgaa gcggcggcga
     5221 gcggtgcgat gaccgggctg aaaatcgccg ctggtgtagc gacggtggta atggcgtttg
     5281 tcgcaattat tgcgctgatc aacggcatta tcggcggaat tggcggctgg tttggtttcg
     5341 ccaatgcctc tctggaaagt atttttggct atgtgctggc accgctggcg tggatcatgg
     5401 gtgtggactg gagtgatgcc aaccttgctg gtagcctgat tgggcagaaa ctggcgatta
     5461 acgaattcgt cgcttacctg agtttctccc catacctgca aacgggcggc acgctggaag
     5521 tgaaaaccgt ggcgattata tcctttgcgc tttgtggttt tgctaacttt ggttctatcg
     5581 gtgttgtcgt tggcgcattt tcggctattt cgccaaaacg tgctccagaa atcgcccagc
     5641 ttggtttacg ggcgctggca gcagcaacgc tttccaacct gatgagtgcg actattgccg
     5701 ggttctttat tggtctggcg taatgctcga tttgtagacc ggataaaacg tattaagtgt
     5761 tgcatccggc aatcgcgcta ttgcaaataa tttttactgc atcatcccgg agaatttatt
     5821 ctccgggtcc atctccagcg ccagtcctga caactgcttt ctgtttttaa ttagatatcc
     5881 tttcttttaa ttaataaacc gtcgtgaatg aattgcgcga gaacatataa aagatgtcga
     5941 taagaaacgc ctaaatactc tgccgcttgc gtatgctttt cgtgataaag atcaccttct
     6001 tgtgagagta atataaatgc tgccaggcga ttcactaacg gaaatgattg attctgagtt
     6061 aaagaaacaa tattacgata atttttatga cttaaggtga cgcaaagttt tcgtaaaaat
     6121 agggtgtcgt ttaataacag cggacggtaa tgtttcatag ggagcgcaag gcaccaacac
     6181 tcttcaatag cctgcaccgc acgcggttca tggtctttgt cgattaactc aatctcgcca
     6241 ataaaacagg gagcggcaaa gaaatcgatc agcgacacgc gaccattggc aagcgtggcg
     6301 taaagcctgg cgcggcctcg cgtcagataa aacagccatg acggctgttg cccttcctgc
     6361 acaatgtagt cacgcgctaa aaaatgaaac agccgcgtat cggctgaaac atccgttgaa
     6421 aagcaatcct taaacgcaga ttcactcatc aattgctgct taaggtcatt attgtggatt
     6481 tctttcacga tgtttatccg ctatgagatt tctcatattt agcatacgta cgctgttgtt
     6541 aagattcaat cattggcccg gtcgaatttt ctttgctgta gaaaaaaata acgcaactgg
     6601 aaacagagga aataaacaat ggaaaagaga aaaattattc tggattgtga gccgggtcat
     6661 gatgatgcta ttgcaatgat gatggcagcg aaacatccgg caatagattt attaggcatc
     6721 actattgtag cgggtaatca gacgcttgat aaaacattaa ttaatggcct gaatgtttgc
     6781 cagaaactgg agattaatgt tccggtttat gcggggatgc cgcaacccat tatgcgtaaa
     6841 caaatcgttg ccgataatat tcacggtgaa accggactgg atggcccggt attcgagccg
     6901 ctgacccgcc aggcagaaag tacccatgcg gtgaaatata tcatcgatac cctgatggca
     6961 agcgatggcg atatcactct ggtgccggtt ggtccgcttt caaatatcgc ggtggcaatg
     7021 cgtatgcaac ccgcaatcct gcccaaaatc cgtgaaattg tgctaatggg cggcgcttac
     7081 ggtacaggca acttcacccc atctgccgag ttcaatatct ttgccgaccc ggaagccgcc
     7141 cgcgtggtgt tcacctccgg cgttccatta gtgatgatgg gcctcgatct taccaaccag
     7201 accgtttgca ccccggatgt aattgctcgg atggaaaggg taggcggccc tgccggagag
     7261 ctgttcagcg acatcatgaa cttcactctc aaaacgcagt tcgaatacta cggcctggct
     7321 ggcggcccgg tgcatgacgc cacctgcatt ggttatctga tcaaccctga tggcattaaa
     7381 acccaggata tgtacgtcga agtggacgtc aacagcggcc cttgctacgg gcgtacggtc
     7441 tgcgacgaac tgggcgttct tggcaagccc gctaatacca aagtcggcat cactattgat
     7501 accgactggt tctggggctt agtcgaagag tgcgtgcgcg gctacatcaa aacccattaa
     7561 gtgttgataa caagccggga cagtatcgca agatgcaggc ccggctattt ctgatgggca
     7621 gaaagccgtc gcccaaaatg acatgaagag ataatcacta tggatgtcat gagaagtgtt
     7681 ctgggaatgg tggtactgct ggcaattgcg tttttactgt cagtaaacaa gaagaagatc
     7741 agcctgcgta ccgttggcgc ggcgttagtg ttacaggtcg tgattggcgg gattatgctt
     7801 tggttaccgc cagggcgttg ggtcgcagaa aaagtcgctt ttggcgtcca caaagtgatg
     7861 gcgtacagcg acgcaggtag cgcatttatc ttcggttctc tggtcggacc gaaaatggat
     7921 accttatttg atggcgcagg atttatcttt ggtttcaggg tattaccggc aattatcttc
     7981 gtcactgcac tggtgagtat tctctactac atcggtgtga tggggatctt aattcgcatt
     8041 ctcggcggta tcttccagaa agctttaaat atcagcaaga tcgagtcatt cgtcgcggtc
     8101 accaccattt tcctcgggca aaacgaaatt ccggcgatcg tcaaaccctt tatcgatcgt
     8161 ctgaatcgca atgaattatt tacagcgatt tgtagtggca tggcctcgat tgctggttcg
     8221 acaatgattg gttacgccgc actgggcgta cctgtggaat atttgctggc ggcatcgtta
     8281 atggcgatcc ctggcgggat cttgtttgcc cgcctgttaa gcccggctac ggaatcttcg
     8341 caggtttctt ttaataacct ctctttcacc gaaacaccgc caaaaagcat tattgaagcc
     8401 gatgcgacag gggcaatgac cgggctgaaa atcgccgccg gtgtagcgac agttgttatg
     8461 gcatttgtcg ccatcattgc gttaattaac ggtattatcg gcggcgttgg cggctggttt
     8521 ggttttgaac atgcctcgct ggagtccatt ttaggttacc tgctggcccc acttgcgtgg
     8581 gtaatgggtg ttgactggag tgatgcaaat cttgccggga gtttgattgg acagaaactg
     8641 gcaataaatg aatttgtcgc ttatctcaat ttctcaccct atctgcaaac ggctggcact
     8701 ctggatgcta aaaccgtggc gattatttcc ttcgcgttgt gcggtttcgc taactttggt
     8761 tctatcgggg tggtggtggg ggcgttttct gcggttgcgc cacaccgtgc gccggaaatc
     8821 gcccagcttg gtttacgcgc gctggcggcg gcgacacttt ctaatctgat gagtgccacc
     8881 attgccgggt tctttattgg tttagcgtag tgtgcaattt atgagccgga ttagatgcgg
     8941 tcaagcgtcg aatccggcaa cattgttaca ccattggcac taatgacagc gcgttatcgg
     9001 cagacagggt agggttgttt aagctgccgc tggcgcgtga aattgccgcg caggccatcg
     9061 caaaacgggc gctgtcacgg aaattgtttc cctccagaaa gctatatacc agacccgcca
     9121 taaaaccatc gtcagcacca aaactgtcta ccgtcgtatg cgctggcaca gtcagcaaaa
     9181 attgttcacc atccttttcg ctgcaataaa ctgactcatc gggcaaataa acaaacagtt
     9241 gctgaacacc ttgctgatgc aatgcattca ctgcggcatt acggtcagca tcgctggtga
     9301 tcgcctgtcc ccataaaatt tccagctccg gtaaagtggg tttcagggtg tgaatatgcg
     9361 ccagccagtg tttgattttg cccgctttga attctgaaac ggtatcgaca aacaccggga
     9421 tctcatcagc gagggtaaag acccattcca gcgcctcggc tgtcaggttg caatcagcca
     9481 gtaccacgcc cgcatgacga agtaaatcgc gcgacccgtt cagtagttgc ggtgtcaact
     9541 gttccagcag atgggtatcg ttaattgcca gcacggtttg atcgtctcga ttggcaattg
     9601 ccagatacgt cgatgtgctt tgaccatgca aacgaacgca gccggagaca ttcacgccgg
     9661 cacggcgcgt ttcttccagg agcatttcgc cataaaagtc atcgccaatc actgaaagca
     9721 aatggacgtc acggcctaac agcgccagat tgtgggcgat attgcgtcca acgccgcctg
     9781 ccgaacaatg aattgtaccg ggatgagaag ccgcttgcgg gtaatggata tccgccatcc
     9841 cgcgaatatc catattgatt gtccccacca ctacgcagta ttcctgctcg gtgagaatgt
     9901 aacctttgtc tttaatccgg cctttgcgca ttaaatccat aatatgcgcc gcaacgcgcg
     9961 aacgactgat ttgcagcatg tccgcaattt cgttctgctg aatcagcggg ttacgccgta
    10021 aaattgcaag gatctccttt tcccggttat tcatcttcag gctaccgctt tttcagtttg
    10081 ttgcgctttc agccaggcga tctcttctgc ccagatatcc gggttgatgg tttcaaggat
    10141 cagcggaatg ccgtcgaaac ggtcgttctg cataatccag cggaacgcat catgaccgat
    10201 attgccttca ccgaggctat gatggcggtc aacgcggctg ccaaaggtac ttttcgcatc
    10261 gttaaggtgc atcccgcgca gatacttaaa gccgacaata cgggcaaaat cagcgaacgt
    10321 tttttcgcat tcggctggag tacgcaaatc atacccggca gcgaaagcat ggcaggtatc
    10381 aatgcagacg ccgacgcggg atttatcttc cacgccgtcg ataatcgccg cgagatgttc
    10441 gaatttaaac cctaagttac tgccctgacc ggcggtgttt tcaatcaccg ctgttacacc
    10501 ttgagtttta tccagcgcaa tgttgatgga ttcggcaata cgcgcaaggc aatcctcttc
    10561 tgaaatctgc atcagatggc tgccagggtg gaagttgagc aaagaaagcc ccagttgttc
    10621 gcaacgctgc atttcatcta taaaggcatc gcgcgatttt tccagagctt cagtgaccgg
    10681 atgtccgagg ttgatcagat aactgtcgtg gggaagaatt tgcgccgatg tgtagtgata
    10741 tttttcacag gcggctttga attcatcgat ggtttgcgtc gtgagcggtg cggcacgcca
    10801 ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg tcgatttcgg cggcgcgaat
    10861 tgcggcattt gccagaccgc cagcagcact aacgtgcgct ccaatgtatt tcatgcgagg
    10921 actcctgtta aacccgctgg aggaaaacgg taatgatagc gggttaacag gagcaagaag
    10981 tagtggttta tgcgatgacg ctttgaatca catagttaat cgcaccacca ccaacaatca
    11041 gccaggcaaa cagtaccagt gccatcagca gaggtttcgc cccagctttt ttcagcgcgc
    11101 tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc cagcaggaag gtatccagcg
    11161 ttaccagcat gttcaccacg ctctgcggta acaggtggaa cgagttaaag atggcaacta
    11221 cgatgaacaa gatggcaaac cacggaatag tgattttgct tttctcgccg ctgttcgccc
    11281 cagacagctg tttaacacgc gccgccagca ggatgaggaa cggagccagc atcatcacgc
    11341 gcagcatttt ggaaataact gctgcgtttt ctgcatccgg gctgatggca tgacctgccg
    11401 ccaccacctg cgccacttcg tgcacagtag aaccaatgta gataccgaaa gtttccggac
    11461 taaaccattg agacatcagc ggatatatcg ccgggtagag gaaaatcgcg acggtcccga
    11521 agataacaac ggttgcaaca gccacggtta ctttactggc ttccgctttc actaccggct
    11581 cagtcgccag taccgcggca gcaccacaga tactgctacc ggcaccgatc aaccaactgg
    11641 tgtgcttatc cagaccaaac actttctgcc ccaggaagca agccagcagg aaggtactgg
    11701 acagcgtcag cacgtcaatg atgatcccac tgataccgac atcggcaatt tgcgagaacg
    11761 tcagacggaa gccataaaga atgataccca gacgtaataa atattgcttg gcaaacagca
    11821 caccaccgtc acagcttttc cagatgtgcg gatagatggt gttgcctaaa accatcccca
    11881 acaagattgc gagggtgagg gcactaaacc cggcacccgc aaccgcggga atggaaccac
    11941 cccacagggc gaccccggtg ataactgcac tcagggctaa ccccggaata aaatgccaca
    12001 gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac cctctccttt aaccgggcat
    12061 aaggttacga ctaactggtt taaaaataaa attgattata tatttataat taatctttat
    12121 aagtggtaag cgactatgca catcaccctc cggcagttgg aagtttttgc agaagtattg
    12181 aaaagtggat caaccaccca ggcgtcggtg atgctggcgt tgtcgcaatc agcagtgagc
    12241 gcagccttga ctgacctgga agggcagctt ggcgttcaac tgtttgatcg cgtggggaaa
    12301 agactggttg ttaatgaaca cgggcggctg ctctatccgc gagcgttggc actgcttgaa
    12361 caggcggttg aaatcgaaca actgtttcgc gaagacaacg gcgcgattcg tatctatgcc
    12421 agtagtacca tcggtaacta cattctgcct gcagttatcg cccgttatcg ccatgattat
    12481 ccgcagttgc cgattgaact tagcgttggg aacagccagg acgtgatgca agcggtgctg
    12541 gatttccgcg ttgatattgg ctttattgaa ggaccgtgcc acagcactga aatcatttct
    12601 gaaccgtggc tggaagacga gctggtggtt ttcgccgcgc cgacttcgcc gttggctcgt
    12661 ggtccggtca ccttagaaca gctggccgct gcgccgtgga tcctgcgtga acgcggttcc
    12721 ggcacgcggg atattgtcga ttatctgttg ctgtcacatt taccgaagtt tgagatggcg
    12781 atggaattag gtaactccga ggcaatcaaa catgcggtgc gtcatgggtt gggaattagt
    12841 tgcctgtcgc gacgtgtgat tgaagatcaa ttgcaggcag gcacattaag tgaagttgcg
    12901 gtccctctgc cgcgcctgat gcgtacgttg tggcgtatac atcatcggca aaaacacctt
    12961 tccaacgcgc tacggcgctt tctggactat tgcgatcccg caaatgtgcc gcgttaagtt
    13021 gctgtacaag aacatgctgg tgctgtgtcg atttcgtgac gcagcgcctt cagcatgcat
    13081 tcgccagaaa agagattggc tgctttactt ataatccctg ggcgatcatg aaggtgtctt
    13141 ataaccgtgt atttctgccg gaaggattgc caatcgtctg ctacaatcgc gccatcattt
    13201 ttaagatgga tagcattttt gtatgggttc cgaaactaaa actacagaag cgccgggctt
    13261 acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc attggcggtt ccatcggtac
    13321 aggtcttttt gttgcctctg gcgcaacgat ttctcaggca ggtccgggcg gggcattgct
    13381 ctcgtatatg ctgattggcc tgatggttta cttcctgatg accagtctcg gtgaattggc
    13441 tgcatatatg ccggtttccg gttcgtttgc cacttacggt cagaactatg ttgaagaagg
    13501 ctttggcttc gcgctgggct ggaactactg gtacaactgg gcggtgacta ttgccgttga
    13561 cctggttgca gcacagctgg tcatgagctg gtggttcccg gatacaccgg gctggatctg
    13621 gagtgcgttg ttcctcggcg ttatcttcct gttgaactac atctctgttc gcggctttgg
    13681 tgaagcggaa tactggttct cactgatcaa agtcacgaca gttattgtct ttatcatcgt
    13741 tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag cctgcgggct ggagcaactg
    13801 gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg atgatcggcg tagctatgat
    13861 tgtcggcttc tctttccagg gaaccgagct gatcggtatt gctgcaggcg agtccgaaga
    13921 tccggcgaaa aacattccac gcgcggtacg tcaggtattc tggcgaatcc tgttgttcta
    13981 tgtgttcgcg atcctgatta tcagcctgat tattccgtat accgatccga gcctgctgcg
    14041 taacgatgtt aaagacatca gcgtcagtcc gttcaccctg gtgttccagc acgcgggtct
    14101 gctctctgcg gcggcggtga tgaacgcagt tattctgacg gcggtgctgt cagcaggtaa
    14161 ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg gcgtgtgacg gtaaagcgcc
    14221 gcgcattttc gctaaattgt cgcgtggtgg cgtgccgcgt aatgcgctgt atgcgacgac
    14281 ggtgattgcc ggtttgtgct tcctgacctc catgtttggc aaccagacgg tatacctgtg
    14341 gctgctgaac acctccggga tgacgggttt tatcgcctgg ctggggattg ccattagcca
    14401 ctatcgcttc cgtcgcggtt acgtattgca gggacacgac attaacgatc tgccgtaccg
    14461 ttcaggtttc ttcccactgg ggccgatctt cgcattcatt ctgtgtctga ttatcacttt
    14521 gggccagaac tacgaagcgt tcctgaaaga taccattgac tggggcggcg tagcggcaac
    14581 gtatattggt atcccgctgt tcctgattat ttggttcggc tacaagctga ttaaaggaac
    14641 tcacttcgta cgctacagcg aaatgaagtt cccgcagaac gataagaaat aagtttcctc
    14701 ccttccttgc taagccctct caaccgagag ggctttttca attccatttc cctgacaaat
    14761 catgcggata taaaatttaa catttggatt gataattgtt atcgtttgca ttatcgttac
    14821 gacgcaatca aaaaaggctg acaaatcaga ggctgttccg gctttctggg atgatcacct
    14881 gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg ggacgtaaag aagatgtgag
    14941 cgatacccat tttattttcg tagttacctc atggagatat ggaatgttta ggttgaaccc
    15001 tttcgtacgg gtcgggctgt gtttgtccgc tatttcttgt gcatggcctg tgttagcggt
    15061 cgatgatgat ggcgaaacga tggttgtcac tgcatcttcc gtggaacaaa atcttaaaga
    15121 tgcacccgcc agtatcagcg tcattaccca ggaagacctg cagcgaaaac cggtacagaa
    15181 tctgaaggat gtcctcaaag aagtgcctgg cgtacaactg acgaacgaag gggataaccg
    15241 taagggcgtt agtattcgtg gtctggacag cagctacacc ctgattcttg tcgacggtaa
    15301 acgcgttaac tcccgcaatg ccgtcttccg ccacaatgat ttcgatctga actggatccc
    15361 ggtcgattcc atcgaacgta ttgaagtggt tcgtggcccg atgtcgtcgc tatacggctc
    15421 cgatgcgctc ggcggtgtag tgaatatcat caccaaaaaa atcggtcaga aatggtcggg
    15481 taccgttacc gtcgatacca ccattcagga acatcgcgat cgcggtgata cctataacgg
    15541 tcagttcttt accagtggac cattaattga tggtgtgctg ggaatgaaag cttacggcag
    15601 cctggcaaaa cgtgaaaagg atgacccgca aaactcaacg accaccgata ccggagaaac
    15661 gccgcgtatt gaaggattct ccagccgcga cggcaatgtc gaatttgcct agacaccgaa
    15721 tcaaaatcac gattttactg ccggatacgg tttcgaccgt caggatcgtg attccgattc
    15781 gctggacaaa aaccgcctgg aacgccagaa ctactgcgtc agccataatg ggcgttggga
    15841 ttacggcacc agcgaactga aatcctacgg tgagaaagtc gagaacaaaa accctggcaa
    15901 cagcagcccg ataacttccg aaagcaatac ggtcgacggc aaatacacgt tgccgctgac
    15961 ggcgattaat cagtttctca cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc
    16021 ggtgaacctg accgggggaa ccagctccaa aacgtctgcc agccagtacg cgctgtttgt
    16081 ggaagatgaa tggcggatct tcgagccgct ggcgctgacg accggcgtgc gtatggacga
    16141 tcacgaaacc tacggtgaac actggagtcc gcgtgcctac atggtttata acgccaccga
    16201 caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa gcaccttctc tgttgcaact
    16261 tagccctgac tggacgagca attcctgccg tggcgcatgt aagattgtgg gtagcccgga
    16321 tctgaaacca gaaaccagcg aaagttggga gctggggctt tactacatgg gtgaagaagg
    16381 ctggctggaa gggggtgaat ccagcgttat cgttttccgt aacgatgtga aagatcgtat
    16441 cagcatcagc cgtacgtctg acgtcaacgc tgcaccgggc taccaaaact ttgttggttt
    16501 tgagacgggc gctaacggac ggcgcatacc ggtatttagc tactacagcg ttaacaaagc
    16561 tcgtattcag ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat ggaaactgtc
    16621 gatcaactac acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca aaccgctatc
    16681 cgatctgccg ttccatactg ctaacggtac gctggactgg aaaccgctgg cgctggaaga
    16741 ctggtcattc tatgtttctg gtcactatac cgggcagaaa cgcgccgaca gcgcgacggc
    16801 taaaacaccg ggcggttata ccatctggaa taccggcgcg gcctggcagg tgactaaaga
    16861 cgtcaaactg cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc gtgacgacta
    16921 cagctataac gaagacggac gtcgttactt tatggcagtg gattatcgct tctgatgaga
    16981 agatgcccgg cgaaccgggc ggactttcac ttcagtaaat actgcgcatg gaagcgcagg
    17041 tgatcctcta taaaagaggc gatgaagtag taactgtgat catatcccgg ctgaatacgc
    17101 agcgtcatcg gccacgcttt ctggcgcgcg gcttctgcca ataccgcagg ttgcaactgg
    17161 tcggcaagaa actgatcatt atcgccctga tcgataagcg tcgggatcgc atcctgcgcg
    17221 ttactggcat acatcagcgc gcaactatcc cattccagca tgcatttttg tcctcaccta
    17281 aataggtgct aaacgctttg attccccacg ggacgctgca cggattcaca atgggcgcaa
    17341 aggccgaaac gctggtgtat ttacccggat ttttcagcgc cataatcagc gcaccgtgac
    17401 cacccattga gtgaccgcta atggcgcagc ggtcgctgac attaaattgc gactgaacca
    17461 gcgccggtaa ttcatcgcgc agataatcat acatccggta atgcgtcgcc cacggcggtt
    17521 gcgtggcatt aagataaaag cctgcgccct ggcctaaatc gtagccatcg tcgttggcaa
    17581 ccttttcgcc gcgcgggctg gtgtctggca tcaccagtac aatccccagt tccgccgcta
    17641 cccgctgggc acccgccttg gtggtgaagt tctcgtcatt gcaggttaat ccggaaagcc
    17701 agtacagcac tggtggcggg gtgtgatcac gagggggggg gagaaagata ctgaacgtca
    17761 tcgggcagtt taaggtactg gagtcgtgtc gccagcgttg ctgccagcct tcaaaacagc
    17821 ggtgctcttc gagcatttcc atgcgtggct ccttgttgtg ttgtttgcaa tttcttcatc
    17881 ataccgttta ttcaatgtgc tgtgagtaac tttcacttcc gtatttgcat aacgatgttt
    17941 taacatctgc tgatgaaagg cagcggcaat tacaataatt atcgctgtga atactggatt
    18001 atgtgcgccg cctcacgcac aataatcaag ctgtaaatca gcttaataac tttgccccca
    18061 cgcagggtgg aggcgtcaca cctgcaggag aaatcataaa tgccatcact cagtaaagaa
    18121 gcggccctgg ttcatgaagc gttagttgcg agaggactgg aaacaccgct gcgcccgccc
    18181 gtgcatgaaa tggataacga aacgcgcaaa agccttattg ctggtcatat gaccgaaatc
    18241 atgcagctgc tgaatctcga cctggctgat gacagtttga tggaaacgcc gcatcgcatc
    18301 gctaaaatgt atgtcgatga aattttctcc ggtctggatt acgccaattt cccgaaaatc
    18361 accctcattg aaaacaaaat gaaggtcgat gaaatggtca ccgtgcgcga tatcactctg
    18421 accagtacct gtgaacacca ttttgttacc atcgatggca aagcgacggt ggcttatatc
    18481 ccgaaagatt cggtgatcgg tctgtcaaaa attaaccgta tcgtgcagtt ctttgcccag
    18541 cgtccgcagg tgcaggaacg tctgacgcag caaattctta ttgcgctaca aacgctgctg
    18601 ggcaccaata acgtggctgt ctcgatcgac gcggtgcatt actgcgtgaa ggcgcgtggc
    18661 atccgcgatg caaccagtgc cacgacaacg acctctcttg gtggattgtt caaatccagt
    18721 cagaatacgc gccacgagtt tctgcgcgct gtgcgccatc acaactgatt aaaaggcagg
    18781 aaccatggag cgcaacgtca cgctcgattt tgttcgcggc gtcgccattc tggggatcct
    18841 gctattaaac atcagcgcct ttgggctacc aaaggcggct tatctcaatc ccgcctggta
    18901 cgatgctatt acgccgcagg atgcatggac ctgggcattt ctcgatctca tcggccaggt
    18961 gaaattcctc acgctttttg cgctgctgtt tggtgctggc ctgcaaatgt tgctgccccg
    19021 tggcagacgc tggatccagt cgcggttaac gctgttagtc ttgttgggct ttattcacgg
    19081 tttattgttc tgggacggcg atattctgct ggcttacggg ctggtggggc ttggcgtttt
    19141 gctgttattg gggctgattt ccgacagcca gaccagccgc gcctggacgc cggatgcatc
    19201 ggctattttg tatgaaaaat actggaagct tcacggcggc gttgaagcga tcagtaatcg
    19261 tgccgatggt gttggcaaca gtttactggc actgggcgca cagtatggct ggcaactggc
    19321 agggatgatg ctcattggtg cggcgctgat gcgcagcggc tggctgaaag ggcagttcag
    19381 cttacgtcac tatcgtcgta ctggttttgt gctggtggcg attggggtga ccattaacct
    19441 tcctgccatc gccctgcaat ggcagctgga ctgggcgtat cgctggtgtg ccttcttact
    19501 tcaaatgccg cgggaactga gtgcgccttt tcaggcgatt ggctatgcgt cgctgtttta
    19561 tggcttctgg ccgcaattga gccgctttaa gctggtgctt gcgatcgcct gcgtcggacg
    19621 gatggcgctg accaactatc tattgcaaac gctgatttgt accacgcttt tttaccacct
    19681 cggtttgttt atgcattttg accgcctgga gctgctggcg tttgttattc cggtatggct
    19741 ggcgaatatc ctcttctctg ttatctggct gcgttacttc cgccaggggc cggtggaatg
    19801 gctctggcgt cagttaactt tgcgtgctgc cggaccggca atatctaaaa catcaagata
    19861 acgatctgga ttacattcgt taacaaaacg gctgtaaccg tttccattgc tgtgactcga
    19921 ttcacgaagt cctgtattca gtgctgacaa aatagccgcc agcaagcagt catttactgc
    19981 aatctcataa caggtagtga atatgatcac cattcgtgat gtagcgcgtc aggctggcgt
    20041 ctctgtggca acggtttccc gggtgctcaa taacagcacg ctggtcagtg ccgacacgcg
    20101 tgaagcagta atgaaagccg tgagtgagct ggattatcgg ccaaacgcca atgcccaggc
    20161 gctggcaact caggttagcg acaccattgg cgtggtggtg atggacgttt ctgatgcgtt
    20221 tttcggcgcg ctggtaaaag cggtggatct ggtcgcccag cagcatcaga aatacgtgct
    20281 aatcggcaat agctatcatg aagcggaaaa agagcgtcac gccattgagg tgttaattcg
    20341 ccagcgttgt aatgcgttga ttgttcactc aaaagcattg agtgacgatg aactggcgca
    20401 atttatggat aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg ggtacgccca
    20461 tcgttgcgtt tgcctggata atctcagcgg tgcccgaatg gcgacgcgca tgttgctgaa
    20521 taacggtcat caacgtattg gttatctttc ttccagcaac ggcattgaag atgacgccat
    20581 gcgtaaagca ggctggatga gtgcgttgaa agagcaggat attattccgc cggaaagctg
    20641 gattggcact ggtacgccgg acatgccggg cggtgaggcg gcgatggttg aactgctggg
    20701 gcgcaatcta caacttaccg ctgtatttgc ttataacgac aatatggccg ctggcgcact
    20761 gacagcatta aaagataatg gcattgcgat tccgttacat ctctcaatca tcggtttcga
    20821 tgatattccc atcgcccgtt acaccgaccc gcaattaacg accgtgcgtt atcccattgc
    20881 ttcaatggct aaattagcca ccgaactggc cttgcagggg gcagcaggca atattgatcc
    20941 tcgtgccagc cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg caacgcgcca
    21001 gaatgcggcg gcgatcacta actcaacaaa tcaggcgatg taaccgcttt caatctgtga
    21061 gtgatttcac agtatcttaa caatgtgata gctatgattg caccgtttta atgttgtaac
    21121 ccgtatgtaa cagtgaataa tcactttcac cgaggtaata gcgtcataac aacaattaaa
    21181 gccgttttct ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg agcttcggcg
    21241 ttcagtaaca cttcattaac tctactgccc cgccgagcat ttatctcaag cactaccctg
    21301 cataagaaaa accggagata ccatgaataa gaaggtgtta accctgtctg ctgtgatggc
    21361 cagcatgtta ttcggtgccg ctgcacacgc tgctgatact cgcattggtg taacaatcta
    21421 taagtacgac gataacttta tgtctgtagt gcgcaaggct attgagcaag atgcgaaagc
    21481 cgcgccagat gttcagctgc tgatgaatga ttctcagaat gaccagtcca agcagaacga
    21541 tcagatcgac gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc tggttgaccc
    21601 ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg tggttttctt
    21661 caacaaagaa ccgtctcgta aggcgctgga tagctacgac aaagcctact acgttggcac
    21721 tgactccaaa gagtccggca ttattcaggg cgatttgatt gctaaacact gggcggcgaa
    21781 tcagggttgg gatctgaaca aagacggtca gattcagttc gtactgctga aaggtgaacc
    21841 gggccatccg gatgcagaag cacgtaccac ttacgtgatt aaagaattga acgataaagg
    21901 catcaaaact gaacagttac agttagatac cgcaatgtgg gacaccgctc aggcgaaaga
    21961 taagatggac gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg ttatcgccaa
    22021 caacgatgcg atggcaatgg gcgcggtaga agcactgaaa gcacacaaca agtccagcat
    22081 tccggtgttt ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat ccggtgcact
    22141 ggcgggcaca gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg atctggcgaa
    22201 aaacctggcc gatggcaaag gtgcggctga tggcaccaac tggaaaatcg acaacaaagt
    22261 ggtccgcgta ccttatgttg gcgtagataa agacaacttg gctgaattca gcaagaaata
    22321 agactgattg ggcgcagtcg tttactgcgc ccttattgta tggccaacaa ggtaaaatta
    22381 tatggtcagc tcaacgactc cgtcttccgg ggaatacttg ttggaaatga gcggtatcaa
    22441 caagtccttt cctggtgtta aggcacttga taacgttaat ttaaaagtcc ggccacattc
    22501 tatccatgca ttaatggggg aaaacggtgc aggaaaatcg acattattaa aatgcctgtt
    22561 tggtatttat caaaaagact ccggcaccat tttattccag ggtaaagaga tcgatttcca
    22621 ttccgccaaa gaggcactgg aaaatggtat ttcgatggta caccaggagt taaacctggt
    22681 attacaacgt tcggtgatgg ataacatgtg gctggggcga tatcccacca aaggcatgtt
    22741 tgtcgatcag gacaaaatgt accgcgaaac caaagcgatt tttgatgaac tggatattga
    22801 tatcgatccg cgtgcgcgcg tcggcacatt atccgtttcg caaatgcaga tgatcgaaat
    22861 cgccaaagcg ttttcctata acgcgaaaat tgtgattatg gatgaaccga cttcttcgtt
    22921 aaccgaaaaa gaggtcaatc atctgttcac tattattcgt aaattaaaag agcgcggctg
    22981 cggtattgtt tatatctcgc ataaaatgga agaaatcttc cagttatgtg atgaagttac
    23041 cgtattgcgc gacggtcagt ggatcgccac cgaaccgctg gcaggactga cgatggacaa
    23101 gatcatcgcc atgatggttg ggcgttctct taatcagcgt tttcctgaca aagaaaacaa
    23161 gccgggcgaa gtcatcctcg aggtgcgtaa cctaacgtca ctgcgccagc cgtcgattcg
    23221 cgatgtctcg tttgatctgc ataaagggga gatcctcggt attgccgggc tggtgggggc
    23281 gaaacgtacc gatattgttg agacgttatt tggtattcgc gagaaatcgg ctggcaccat
    23341 tacgttgcac ggcaaacaga tcaataacca taatgccaac gaagccataa accacggatt
    23401 tgcactggta actgaggagc gccgctcaac gggaatttat gcctatctgg atattggttt
    23461 taactcgtta atttccaata ttcgcaacta caaaaataaa gttggtttac tggataactc
    23521 gcggatgaaa agcgataccc agtgggtgat tgattcgatg cgggtaaaaa cgccgggtca
    23581 tcggacgcaa attggttcgc tctccggtgg taatcagcaa aaggtgatta ttggtcgctg
    23641 gctactaacg caaccagaaa tattaatgct cgatgaaccg acgcgcggta ttgatgtcgg
    23701 ggcgaagttt gaaatttatc agttaattgc cgaactggcg aagaaaggca aggggattat
    23761 tattatctcc tctgaaatgc ctgagttgtt agggataaca gaccgtattc tggtcatgag
    23821 caatggtctc gtttccggaa ttgtcgatac aaaaacaaca acgcaaaacg aaattctgcg
    23881 tcttgcgtct ttgcaccttt aagattaggg gcttcccatg agtgcgttaa ataagaaaag
    23941 ttttcttact tacctgaaag agggcggtat ttacgtcgtt cttttagttt tgctggcgat
    24001 tattattttc caggacccaa catttttaag tctgttgaac ttaagtaata ttctcaccca
    24061 gtcatcggtg cgtattatta tcgcgctcgg tgtggcaggg ttaattgtca cccaggggac
    24121 cgatctttct gctggtcgtc aggtagggct ggcggcagtg gtggctgcga cattattgca
    24181 gtccatggat aacgccaaca aagtgttccc ggaaatggcg acgatgccga ttgcgctggt
    24241 tattctgatt gtctgtgcca ttggtgcggt gatcggtttg atcaacggtc tgattatcgc
    24301 ttatctcaac gtgacgccgt tcattaccac gctcggcacg atgatcatcg tctatggcat
    24361 caactcgctc tattacgact ttgtcggggc gtcgccaatt tctggttttg acagtggctt
    24421 ctctaccttt gctcagggct ttgtcgcgct ggggagtttc cgtctctctt acatcacctt
    24481 ctacgcgttg attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc gcttcggtaa
    24541 gaacattttt gccattggcg gtaacccgga agcggcaaaa atatctggtg tcaacgtcgg
    24601 cctgaacctg ctgatgatct acgcgttgtc tggcgtgttc tatgcctttg gcgggatgtt
    24661 agaagccgga cgtatcggct ctgccaccaa caacctcggc tttatgtatg agctggatgc
    24721 tatcgcggcg tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga cggtgattgg
    24781 cgtggtgacc ggggtaatta tttttaccgt catcaactat ggcctgacgt atatcggcgt
    24841 aaacccatac tggcagtaca tcatcaaagg ggcgattatt atcttcgccg tagcgctgga
    24901 ttcactgaaa tacgcgcgta agaagtgatg tgattgcgga tattgccgga tgcgtctctg
    24961 gttttacaac gttaccgggt gttctttctc gcctttctta aacttcactt ccccgagctc
    25021 aatacaaccc accgggcaga cgtgaccaca aagcagacaa cccacacatt tctcggtatt
    25081 acaatgcggt gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca
    25141 ggaaatataa cagcgtccac agccaacaca tttgtcaaga ttgatacggg gatagacaat
    25201 ataactgcgg tctaaatctt ccgccgggac aatattgtta ttcgccagac ctaccatctc
    25261 ctgcaacgaa tcaaatccct gatcggcgag ataatgcgat aacccgctcg ccatatcttc
    25321 tacgatgcga tagccgtact gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag
    25381 caataaaaac tccgccgcat cttcccaggt ttcaatgccg ccgataccgc tgattgggaa
    25441 atcgcgcagt tctggatggg tgcgcatttg ctgaatgaag cgcagggcga tcggttttac
    25501 cgctttaccg gaatatccgg aaatactcga tttaccgtta acgatcggca taccgatttt
    25561 ctgattaaga tcgatattgg tgatggattt aacggtgtta attgccgcaa tgccatctgc
    25621 gccgccgcgc ttcgccgcca gcgccacttc gcacatatca ccgatattcg gcgtcatctt
    25681 cgccagcatt ggcagcgtgg aaccccgttt cactgcccga caatattttt ctaccagctc
    25741 cgggctttgc ccgacatcgc tacccatcgc atgagaagtc atttgcggac aggagaagtt
    25801 acactcgatc atatccgcgc cagctgctcc cattgctgct cattttcccc catgatcgaa
    25861 gcgatcaata ctttgtccgg gtaatcttcc ttcagccgac gcagggcggt cagattctct
    25921 tccaacggat gttcagcaat ctgctccata tttttgaagc cgataaaacc ggtatcttct
    25981 ttcaccagat gatcaaaacg cggcgagact tcgttggcga taaaaaagcc gatcgtttta
    26041 aacaccacac cgccccaacc tgtgtcgtag gctttggcac acatctcata gcagttgcct
    26101 accggcgaag aagagaggca gaacgggttg ggaaagttca cgccgcaaaa agtaatcgaa
    26161 agatctttcg ttaacatgag caagctccct ctaaatagtg atgaatcgcc tcggcggctt
    26221 ctttcccggt tttcacggca tagaccacgg ttttgtcacc ctcaacaata tcgccagcag
    26281 caaagacttg cggatcgcgg gtctgataat tttgtgtttt gatggtgtta cgctgctgct
    26341 ctaactccgc aaaggcatcc agtctggcat gctgaccgac ggcgagaata attttatctg
    26401 ccgccatcgt cagttcgcct gataaccgta catgcttaaa cgtgacttta ttgccttcca
    26461 cggctactgg cgtgaatcca tcgatgatcg aaacgcccag ctcccgggcg ctggcaaact
    26521 ctttttcgct tgccggaaac tcatctaact cttcacgtgc tacgcaagtt accgcctgac
    26581 agccgagaac tttcagcgtg ctggctacgt ccatcgcgac atcaccgccg ccgataatta
    26641 atgcgctttg tggaatgctg atatcgcctt gcgcctgtcg tacacgttgc aggaagtcga
    26701 cggcaatctc aacgtcgcta tgctcaaaca gcggtagccc ggaaccgctt gataacccca
    26761 cggtgaccag taccgcgcgg ttttccgctt tcagttgctc cagggtgagt gttttaccga
    26821 tttcgttatt gcacttaatg gtcacgccca ttttttcaat acgggcgatc tctgcatcca
    26881 gcactgattg cggtaaccgg aattgcggaa taccgttacg caaccagcca ccggggtgcg
    26941 cttctttctc ataaatcgtg acgtcataac cctggtttgt cagtgtcaca ctggcctgca
    27001 atccggcagg acctgcgcca ataatcgcga ctttgccgag cgttttagta ccgggctgat
    27061 aaatttccat cccggtttgt tgttcaaaat cagtaacaaa acgctgtaag cggccgatat
    27121 caatgggagc atcaacaccg gcacgggtac aaccgctttg gcataatttc tccgccgggc
    27181 acactctggc acaaacggca ccgagggcat tattttcgcg aattgtctcg gcagcgcctt
    27241 taaaattacg aaagtagatt gagcgaataa atttccccgg atcggtctgt gccgggcaag
    27301 cctgactaca gggagcgtcg tgacataata aacagcgaga ggcttcttta atcgccagta
    27361 acggcgtaaa agccggagtg agttcatcca gataattttg ttgcggcatg tgatcttcct
    27421 taatgctatg agtaattatg gcgaacgcta aataaatatt gcgtgctgat attaaggcta
    27481 tgcgcgtggg gaaatttaag ctgtgaaagg attcacaaat agctgcggtt cagaatgtta
    27541 tatccgcagc ttattgagtg aagtaataat tattctcaat aattaacaga aaagagaaaa
    27601 tattaacgat agaaaataag ataaacaatc atgccgatag ataaaattcc gaagccaata
    27661 tatgccacca tataagcgaa gaagagtccg ggattttttg ctcgccagac atacaccggt
    27721 tccggtgcat ttttaacccg tttatcaatc gcgcctgcgc gccagccttt ccatgcttcg
    27781 cggaaacaaa atatcgggat aaggaaaaaa acggcaacga caaaaaactg ctgtacatcc
    27841 ataactactt tccttgtttc ttttgtaatt caagtaactg ttcgggtgta acggcgggat
    27901 acccctgcgc atcttccggc acctggtgca tagccggaat agggaccagc ggccctaaaa
    27961 aacgcggttc acgtttaaaa atataaaggt cagccagcgc accgaaacgg gcggcaaatt
    28021 cgcgaatacg tactgacagc atatctttcg gtgacggtac ggcataacac tgagcctgaa
    28081 tccccatatg cagcgcaata aataatgctc gctcacagtg gaaacgttgg gtgataataa
    28141 tgaaatcatt agtatcgaaa actttgcgtg tacgcacgat ggaatccagc gtacgaaagc
    28201 ctgcgtaatc gagaacaata tctgatgggt cgacaccagc agcgattaaa tctttgcgca
    28261 tggtcatcgg ctcgttataa ctttgcaatg cgttatcgcc gctcagtaat agataattta
    28321 ccttaccgct gttataggca ttaatcgctc cttgaatgcg gtagcgataa tactgattaa
    28381 taacgccggt acgataatat tttgctgtgc cgagcaccac gccgacctgg cggtagggga
    28441 gatcctgcaa ttcgtcgtag atataaggcg cggttttcca gctcatccag cgatcgaggc
    28501 cgagcacagt caacagcagc aagccgatca ggactaacag gctgaggaac acgcgcttta
    28561 acatgcagga gactcgagct aaggaaaatt tccatcaggc tactttaccc gctggttttg
    28621 cgcaagaaac ggcaaactgt ttgccgggaa tttcgacacc aggagagggc tggcaagccg
    28681 gacaaggcgt tcacgccgca tccggcacca ggcttaagcg agaagcacgc ggtcgatact
    28741 gtgacagccg agagctttca gcgttgcgga ggtggcatcc cactgaatca acggcgcatc
    28801 ggctttttct gccagaaccg cgcgctggat atccgggtaa tcataaccct tgagattcaa
    28861 cagaattaac gccccctgca acggcggcag agtcgggttg aatgcggcgt tttcagcgta
    28921 gctgccgctg aaaatacgac cgtctttaca ttccagcgcg acaccgcttg gtgacttact
    28981 gtaaggcatg tgcgaacggt ttgccgctgc aatcgccgcc tgagaaagcg catcaccagt
    29041 cagcgcatag ccgtgatcct gctcgtccat cagcagcgtt ttaatctcca gatctttcgg
    29101 cccaaaggca tccggcagat agtcacgcag cgcgtgtgcc tcgcggcccg gcaaatgaat
    29161 acgcagatcc agaccgctgt tcagttcatt cataaactga cggcagtgac cacaaggcgt
    29221 atagttaacg gtgatggctg caagcgcttt ttcaccactc aaccaagagt ggctgatcgc
    29281 gctttgttcg gcatgaacgg tttgctgcat tgttgcacca ataaattcca tattggcacc
    29341 aaaataccag gttccgctca caccgcgcgc aattgcgcca acattaaaat tcgacaatgg
    29401 cgtacgcgca caggccgccg ccagcggaag tagtgcgaat gccagcgcgt cttcgtccag
    29461 tcccgttgcg ctcttcagcg atgagacttg ctccccggtc aacaaagcgg ggaagtactt
    29521 gtctgccaga ataggttcca gtgcagattg caagttatcc gcaagttggg caaaagcggt
    29581 ttgaaaacgt ggatgcatgg cgttgcctca taatcatgta atgggataac agtttacgaa
    29641 cccgttatgg ctttatatgt gatctgaatc tcattaatta tgcaacacgt gaaacttaca
    29701 tcaaaaatgc gcgacgtatc gcaaatttta gcccattact gccagaataa tcgggaaaag
    29761 gaacggtgcg atcagcgaag taattatccc gcataacacc agcgctagcg aactaaatgc
    29821 accttcctga taatccagct cggcgcagcg cgccgtaccg agggcgtgcg aggcagtccc
    29881 cattgccaga ccgcgcgcag ctttggtacg aatacgcatc gcattaagca atgtatggcc
    29941 aaataccgcg ccgaggatgc cgacgaaaat cacgcaaact gcgctgattg ccggaatacc
    30001 gccaatactg ccgccaaccg ccattgcaat aggcgtggtg actgatttcg gcaggattga
    30061 cgcggcgatt tccggtgaag cgcccatcaa caatgccacg gaagtccccg tcaccatcgc
    30121 aaccacgctg ccgataaaac agatggtgat gatcgatttc cagcgcgcgc ggatctggtg
    30181 tagctgctca tataaaggat aggccagcgc gacgaccgcc ggttgcagca gatcgtttag
    30241 cacttcgcta cctttaaagt aactgtcgta agagatgccg gtcagcatta aaaaaggaat
    30301 gatcaccacc attgctacca gcaacgggtt aagcagggga aacttatacc gtgccgccag
    30361 tttgcgggcg gcaaaaaaga caatcaacgt cagcggtaat gaccaccaga tattcgccat
    30421 cattcttctg atcctttctg acctacgact ttacgttcac cgtgtaccag ttgtgaactc
    30481 cagctcacca ccagaaaaac caccagcgta ctgacagcac aggaaaccac taccgggcca
    30541 aactgcgcgc ggagcaaatc aaaatattgc atgacgccta cgccaatcgg cacaaacaat
    30601 agcgccatat agcgaatcag tacgtagcac cccggattga cccatttcgc cggaagaatt
    30661 tgcaaggcca gcaggacaaa caggatcagc atcccgatga tgctgcccgg aatggttacc
    30721 ggtagcaggg aagcaatgaa aatgcctgca tacaggcagg cataaatcag gacgaaagcg
    30781 cgtaaatatt gccagataat gttcagtgtc ttactcataa tcaacacccc agatgagatg
    30841 gattcatcat acaattaatt cgtaaaacgt gctaccgatc acattaagaa atcagcgctt
    30901 attttttcgc tacccggtat tacgcaatga gcagcgaact caggcataca tcaagcatag
    30961 agagggattg cagtttgcag caatccctct ttgcagacct ttaatccgct gaccataggc
    31021 gctaacatag cgcctcattt ttttgcgggt gatgatatgc gtgtgttact ggcaccgatg
    31081 gagggagtgc ttgactctct ggtgcgtgaa ttgctgaccg aagttaacga ctacgatctg
    31141 tgcatcaccg agtttgtccg cgtggtggat caactgctgc cggtaaaagt ctttcatcgc
    31201 atttgccctg agctacaaaa cgccagccgg acaccatctg gtacgctggt gcgcgtgcag
    31261 ttgttaggtc agttcccaca atggctggca gagaacgccg cccgtgcggt ggagttaggt
    31321 ttctggggcg tggatctcaa ttgcggctgc ccgtcgaaaa cggttaacgg tagcggcggc
    31381 ggggcgacgt tactcaaaga tcctgaactc atctaccagg gggcaaaagc gatgcgtgaa
    31441 gctgtaccgg cgcatttgcc tgtcagcgtg aaagtgcgtc tgggctggga cagcggtgag
    31501 aagaaatttg aaatcgccga tgcggttcaa caggctggcg ctacggagct ggtggtgcat
    31561 gggcggacga aagagcaggg ttaccgcgcg gagcatattg actggcaggc gattggcgag
    31621 attcgccagc ggctgaatat tccggtgatt gccaacggtg aaatctggga ctggcagagc
    31681 gcgcaacaat gcatggcgat cagcggctgc gacgcagtga tgattggtcg cggggcgctc
    31741 aatattccca acctgagccg ggtggtaaaa tataacgaac cgcgaatgcc gtggccggag
    31801 gtggttgctt tgctgcaaaa atatacccgt ctggaaaagc agggcgatac cgggttatat
    31861 cacgttgcgc ggattaaaca gtggttgagt tatttgcgta aagaatacga tgaagcaacg
    31921 gaattatttc agcatgttcg ggtgttgaat aactcccctg atattgcaag ggctattcag
    31981 gcaattgata tcgagaaact ctaaaaacca tagtgatgtt tttgacaatg tgttagctct
    32041 ttatcaatag gccgcacggt tgtttggtgg cgtcaaaatc gccgaggcgt tccctgaagg
    32101 ccggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc
    32161 ccgacccggt agccgtaagg gataagccga gggcaccgcg cagcggcgat tttgttcgcc
    32221 aagcccgggg gggcaggggg cggcggcgat tggtcgcccc ctgcgcgctc cttgcgccag
    32281 tgattatatg aagcaatgca catgacagga gcgcaacagg atgagagact gaaatattct
    32341 cgttattttc ggatctgatg acattaatta attttaaaat atcatcttaa atacgccagt
    32401 caccaccagt agcccaatca ggaaaataat caatacagcc cagagtataa ttttcattgg
    32461 cgttccttta tttgtgagtg ctgaataagt ataggaaaaa acgaagccct ttgccgttat
    32521 ccataaacga ttaagataaa tcggaagata tcatttactt agtctatatt tttctgggta
    32581 tgtcgtttta ttgctactga tgtattgcca cgcgaaacta aaaaatatat cgcaacacac
    32641 ggtaatctcc gcgcaccgtt tcttacctga ttattccccc atgaatcgtg attcctttta
    32701 tccagccatc gcctgttttc cgctgttact gatgctggcc gggtgtgcgc ctatgcatga
    32761 aacccgccag gcgttaagcc agcaaacgcc cgctgcacaa gttgacaccg cattacccac
    32821 ggcgctgaaa aatggttggc cagacagcca atggtggctg gagtatcacg ataatcagct
    32881 cacttcatta attaacaatg cactacagaa cgcaccggat atgcaggtgg ctgaacagcg
    32941 catttagcta gctgaagccc aggcgaaggc agttgccact caggatggtc cgcagatcga
    33001 cttttcggcg gatatggagc ggcaaaaaat gtcggcagaa gggctaatgg ggccgtttgc
    33061 tctgaacgat ccggccgcag gtacgaccgg tccgtggtac accaacggaa cttttggctt
    33121 aacggcgggc tggcatctcg atatctgggg aaagaatcgg gcggaggtta ctgcccgcct
    33181 gggtacggtt aaagcacggg cggcggaacg cgagcaaacc cgccaattgc tggctggcag
    33241 cgtagcccgc ctgtactggg agtggcaaac ccaggcggcg ttaaacacgg tcttgcagca
    33301 aatagaaaaa gagcagaaca ccattatcgc gaccgatcgc cagctatatc agaacgggat
    33361 tacttcttca gttgacggtg tggaaaccga tattaatgcc agcaaaaccc ggcagcagct
    33421 caacgatgtc gcggggaaaa tgaaaattat tgaggcacgg ttaaatgcac tcacaaatca
    33481 ccagactaag tcattgaagc ttaaaccggt cgcgttgccg aaagtggcaa gccagcttcc
    33541 tgatgaactg gggtactcct tactggcccg gcgggcagat ttgcaggcgg cgcactggta
    33601 cgttgagtca tcgttaagca ctattgatgc ggcaaaagcg gcattttatc ctgacatcaa
    33661 cctgatggcc ttcctgcaac aggatgcgtt gcacttaagc gatctgttcc gtcattccgc
    33721 gcagcaaatg ggcgttacgg caggcctgac gctacctatt ttcgatagtg gtcgtcttaa
    33781 cgccaatctc gatatcgcaa aagccgaaag caacttgtct atcgccagct acaacaaagc
    33841 ggtggttgaa gcagtgaatg acgtggcgcg ggcagccagt caggttcaga cactggcgga
    33901 gaaaaaccag catcaggcgc aaattgagcg cgatgccttg cgtgtggtag gtcttgcgca
    33961 ggcccgcttt aacgcgggca tcattgctgg ttcccgcgtc agcgaagcca gaatcccggc
    34021 gctgcgtgag cgggccaatg gcctgttatt gcaagggcag tggctggatg cctccattcg
    34081 actcaccggt gcgctgggcg ggggttacaa acgctgatga tatgcgcctt ctatacttaa
    34141 cgtttattca gcgttaagtg gagaactcga tggcataggt cgcgataatt accgcttccg
    34201 attcggggat cggcaaagag tgcgcgttat tactggcgca gcaggggttt gatattggca
    34261 ttacctggca ctcagatgaa gaaggggcaa aagataccgc gcgtgaggtg gttacccacg
    34321 gcgtacgtgc ggagatcgtg caactggatc tcggcaatct gccagaaggt gcgcaggcgc
    34381 tggagacact catccagcga tttgggcgta ttgatgtgct ggtgaataat gcgggtgcaa
    34441 tgaccaaagc gccgtttctt gatatggctt ttgatgagtg gcgcaagatt tttaccgttg
    34501 atgttgatgg tgcattttta tgctcgcaaa ttgcggctcg tcagatggtg aaataagggc
    34561 agggcggtcg catcatcaac attacgtcgg tacatgaaca tacgccgctg ccggatgcca
    34621 gcgcctacac agccgctaaa catgcgctcg gcgggttaac caaaacgatg gcgctggagt
    34681 tggtcaggca taagattttg gtgaacgcag tcgcgcctgg ggcgatagcc acgccaatga
    34741 acagcatgga tgatagcgac gtgaagcccg acgcggagcc ttcgattcct ctacggcgtt
    34801 ttggcgcaac gcatgagatt gccagcctgg tggtgtggct ttgttcggag ggcgcaaatt
    34861 acacgacagg gcagtcgttg atagtggatg gcggctttat gttggcgaat ccgcagttca
    34921 acccagaata gcgcagtgag gtgcgtggat atttcaatgg gttaggccag ataagacacc
    34981 agcgtcgcat caggcatgga acacccggtg ccggatgtgg agtgaatgct tatctgtggc
    35041 aactgcttta cgcctgatta tccggctttt tcttcccgcg ccgtttcagc caccagcgca
    35101 cgcccaccac cagaaccacg accagaatca accagaccca gtgcttcaaa tgctggtcga
    35161 gattgtgcaa ccacggcgca atcacctgac caccagcgta accaatagtg gtaaaaatca
    35221 acgcccaggc aaatgcgccg agaatattca gcggcagaaa gattttcggc ggcagctggc
    35281 tggcaccaat cagcgtcggg ccaatcaccc gaaagccata cataaagcgc gtaccaatga
    35341 caaacagata cggatggcgt tggataagtt tctgtgcccg ctcaatttta tcctgatgtt
    35401 tcgagaaacg gcgtaacagc ttgccgccaa accgccgccc gcacagatag agcacctggt
    35461 cgccaatcat gccaccaagc gccacagaaa gtaccaccag tgggaacttt aatagcccct
    35521 gatgtgccgc aacgcctccc agcaaagtca cggtttcacc ttccgccagg ctaccgataa
    35581 ccagcgcggc ataaccatat tgtgagatga gtgtattgag atccataggc cagtttgtct
    35641 ccctttggtt gcctgtaagc atacaccctg tgaatcattc acgctgaaaa aggacataca
    35701 tcagacggtg caaattacat ggatgaacaa aaaataatca caagctggct tatacttgaa
    35761 ctgtcaggtt catgaactca caacaagggg gcggtatgag ccatgtctgg ggactgtttt
    35821 cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa cgaaacgatt tctcaccatt
    35881 acacccacca cgttttgctg atggcggcga ttccggtgat ttgcgccttc attggcacta
    35941 cacagattgg ctggaatttt ggcgatggca ctatcctgaa gttatcctgg tttaccggac
    36001 tggcgctggc tgtcttgttt tacggcgtta tgctggctgg ggttgcggtg atggggcggg
    36061 tcatctggtg gatggcgcgc aattacccgc agcgtccgtc tcttgcgcac tgcatggtct
    36121 tcgcgggcta cgttgcaact ccgctgtttt taagcggtct ggtggcgctt tatccactgg
    36181 tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta caccggctac ctgctttacc
    36241 ttggtattcc gagcttcctg aatatcaaca aagaggaagg ccttagcttc tccagttcga
    36301 cgctggcgat tggcgtgctg gtactggaag tgctgctcac cctaaccgtt atactatggg
    36361 gctacggtta tcgtttgttc tagaaagttc tttgacgttg cattgctggc gtaaatgtca
    36421 gcaatgcagc atttactcac gattctcctt tgacgatcgc actttttgct cgttatgatg
    36481 gcgtcgctag cctcagtaaa tccttactcc ggcggtgcgc aacccgtgcg cgtgaaccac
    36541 tatctgaatg ctcatcatgc cgaaatttcg agtttcttta tttagcttgg ccctgatgct
    36601 ggctgtgcct tttgcaccgc aggccgttgc gaaaacggca gccgctacca ccgcttcaca
    36661 accggaaatt gcctccggta gcgcgatgat tgttgatctg aataccaaca aagtgatcta
    36721 ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc agcaaattaa tgaccgcgat
    36781 ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta aaagtggata tcagccagac
    36841 gccggagatg aaaggggtct attcgcgcgt acgactgaat agcgaaatca gccgtaaaga
    36901 tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg gcggcaagcc ttgcgcacca
    36961 ttatcccggt ggttacaaag cctttattaa ggcaatgaat gcgaaagcga aatcgctcgg
    37021 aatgaacaac acgcgctttg ttgaacctac cggattgtcg gtgcataacg tttcaactgc
    37081 ccgtgactta accaaactgc tcattgccag caaacaatat ccgttgatcg ggcagttaag
    37141 tactacccgg gaagatatgg caaccttctc taacccgacg tatacgctgc cgttccgcaa
    37201 tactaatcat ctggtgtatc gcgataactg gaatattcag ttaaccaaaa ccggctttac
    37261 caatgcggcg ggccattgtc tggtgatgcg tacggttatc aataataaac cggtggcgct
    37321 ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc gatgccagcc gcctgcgtac
    37381 ctggattgaa accggtaaag tgatgcctgt gccggcagca gcgttgagct ataaaaaaca
    37441 aaaagccgcc caaatggcgg cggcggggca gacggcacag aacgattaag tgaattcgga
    37501 tggcgatact ctgccatccg taatttttac tccacttcct gccagttttt ccgtttactg
    37561 gttttaccga tccccggatt catgctattg gtcggatcat tctcgcgata gaacttctgc
    37621 aacgtctccg gtgctttata caaatgaccg acgttatgct cggcagggta ctgcgcgccg
    37681 cgctgctgta gcagttccag catctgttct tttaacgcat gcacatccac gccttttttc
    37741 actatgtaat cctgatggaa gacatagcac ataaaatggc cgtaatagag cttgtgcacc
    37801 agctggctgt cgatctccgg cggtaaatgc tcataccact cggtgtcgtt acgccgcaga
    37861 gcgatatcca gtgccagaat gtcttcgact tcatcggaat gcaccgcctg ataacgaatt
    37921 gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc taccttcctc cggcgtacag
    37981 acaaagaaat cgccctcggc ctgtttgaaa taatccacca gccacgattt ggcttcaccg
    38041 acgccatcgc ctgccatttt taacagcaga tgatgctcgt atttatcgcg ccagtttttc
    38101 atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt tttgcattgc acggtcagta
    38161 aaatgcggac ggaagaattt cactttctcc agcatcgcat cggtgcgtcc cttgagatta
    38221 aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca tcaggaaggt gtctttgccg
    38281 tatttttccg caatatcgta gatatcccgg tgcatatatt ccccggcaac cggcagattt
    38341 tcgaagttag ccagaatatg acggcggatt tcggtcagca cttccggctg gttggtgccg
    38401 atataaaaca cctgctgatt tttttccgct tcgaaggtat caagacgtac cgcaaagacg
    38461 gccagcttcc cggcgcaacc agaagattca aataaccgat ccggatcggc gttataacgt
    38521 gcgggcgtgt cggcttcaat atcacgaacg cggtggacat agtcataatc gtgggcgtga
    38581 cgaccatcgt gacgcacatc gtcatctttg atgcgatcgt catccagctt gctaaggatc
    38641 tgctccggcg tttcgcccag atcaatcccc agatggttca ccagcgtcag tttgccatct
    38701 tcatctatac gcgcgaataa cgacatttcg gtatacgccg ggccacgttg caccagcgag
    38761 ccgccggagt tgttacaaat accgccgatg accgatgcgc ctatacacga tgatccaatc
    38821 accgaatgcg gttcgcgtcc cagcggtttg agggcttttt ccagcgaata gagcgtggtg
    38881 cccggatagg ccagcacctg ttcgcccttg ccgagaacgt gcagcttgtc gagacgcagg
    38941 gtgctgatga tcacgatatc gcgatcatag tcgttaccgt ttggcgtcga tccttcggtc
    39001 aggcctgtat tggcagcctg catcagaata attttgtcgg cggtgacgca ggctttcagc
    39061 acccgccaca attccagtag tgagccaggg aaaacgacag ccagcgcgtc gccctgacca
    39121 gaacggaagc ccttgcgata gcgggccgtt tttgcgggat cggtgagcag gtgtgaatga
    39181 ccgaccagac gggcaagttc attcaaaaag gctttattat cagttgttgt catggaagac
    39241 attttccact ccttgtggtg gcgaaaaaat atcacaaact agaatagcgc cttgtatgac
    39301 aattcagcga gtgaatattt tgaaaattca gacaataaac tgagcattct ttttctctct
    39361 ccatcatgct tatggcaaac tacggttttt gcaccatttt cgctgccacg tcgggcaaca
    39421 aaggaagaaa aatccatatg aaatggctat gttcagtagg aatcgcggtg agtctggtcc
    39481 tgcagccagc actggcggat gatttattcg gtaaccatcc attaacgcct gaagcgcggg
    39541 atgcgttcgt caccgatctg ctcaagaaaa tgacggttga tgagaaaatt ggtcagctgc
    39601 gcttaatcag cgtaggcccg gataatccga aagaggcgat ccgcgagatg atcaaagacg
    39661 gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga tatccgcgcc atgcaggatc
    39721 aggtgatgga attaagccgc ctgaaaattc ctcttttctt tgcttacgac gtgctgcacg
    39781 gtcagcgcac ggtgttcccg attagcctcg gtctggcctc gtcttttaac ctcgatgcgg
    39841 tgaaaacggt cgggcgtgtc tctgcttatg aagcggcaga tgatggcctg aatatgacct
    39901 gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg acgtgcttcc gaaggttttg
    39961 gcgaagatac gtatctcacc tcaataatgg gtaaaaccat ggtggaagcg atgcagggta
    40021 aaagcccggc agatcgttac tcggtgatga ccagcgtcaa acactttgcc gcatacggcg
    40081 cggtagaagg cggtaaagag tacaacaccg tcgatatgag tccgcagcgc ctgtttaatg
    40141 attatatgcc gccgtacaaa gcggggctgg acgcaggcag cggcgcggtg atggtggcgc
    40201 tgaactcgct gaacggcacg ccagccacct ccgattcctg gctgctgaaa gatgttctgc
    40261 gcgaccagtg gggctttaaa ggcatcaccg tttccgatca cggtgcaatc aaagagctga
    40321 ttaaacatgg cacggcggca gacccggaag atgcggtgcg cgtggcgctg aaatccggaa
    40381 tcaacatgag tatgagcgac gagtactact cgaagtatct gcctgggttg attaaatccg
    40441 gcaaagtgac catggcagag ctggacgatg ctgcccgcca tgtactgaac gttaaatatg
    40501 atatggggtt gtttaacgac ccatacagcc atttggggcc gaaagagtct gacccggtgg
    40561 ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg tgaagtggcg cgcgaaagcc
    40621 tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa aaaatcggcc accattgcgg
    40681 tggttgggcc actggcggac agtaaacgtg acgtgatggg cagctggtcc gcagccggtg
    40741 ttgccgatca atccgtgacc gtactgaccg ggattaaaaa ttccgtcggt gaaaacggta
    40801 aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa aggcattatc gatttcctga
    40861 atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc gcaagagatg attgatgaag
    40921 cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt agtcggtgaa gcacagggga
    40981 tggcgcacga agcctccagc cggaccgata tcactattcc gcaaggccaa cgtgacttga
    41041 ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt gctgatgaac gggcgtccgc
    41101 tggcgctggt gaaagaagat cagcaggctg atgcgattct ggaaacctgg tttgcgggga
    41161 ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga ttacaacccg tccggcaagc
    41221 tgccaatttc cttcccgcgt tctgtcgggc agatcccggt gtactacagc catctgaaca
    41281 ccggtcgtcc gtataatgcc gacaagccga acaaatacac ttcgcgttat tttgatgaag
    41341 ctaacggagc gctttatccg ttcggctatg gtctgagcta taccactttc accgtctctg
    41401 atgtgaaact ttctgcgccg accatgaagc gtgacggcaa agtgacggcc agcgtgcagg
    41461 tgacgaacac cggtaagcgc gagggggcca cggtagtgca gatgtacttg caggatgtga
    41521 cggcttccat gagtcgccct gtgaaacagc tgaaaggctt tgagaaaatc accctgaaac
    41581 cgggcgaaac tcagactgtc agcttcccga tcgatattga ggcgctgaag ttctggaatc
    41641 aacagatgaa atatgacgcc gagcctggca agttcaatgt atttatcggc actgattccg
    41701 cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt tagtaagggc gcgacgtttg
    41761 cgccctttgc ctgatgttca tatcttaact tgctgaattt tcgccctttg taggccggat
    41821 aaggcgctcg cgccgcatcc ggcaggaaac gccggaccga atatgcctct taagtattta
    41881 tctgattctg caactgaatc cttccgctca ggctaactct gtcattatca actatgcttt
    41941 tctcttaatt cgctgaaaac aacagtaaaa gaatgaggaa agcatcatgc cactcttaaa
    42001 gctctgggca ggttcactgg ttatgttggc agccgtgagc ctgccgctac aggcggcttc
    42061 ccccgttaaa gtcggttcaa aaatcgatac cgaaggcgcg ctgctcggca acatcatctt
    42121 gcaggtactc gaaagccacg gcgtaccgac ggtgaataaa gtgcaactcg gaacgactcc
    42181 tgtggggcgc ggggcgatta cttccgatga actggatatc tatccggaat ataccggcaa
    42241 tggcgctttc ttctttaaag atgaaaacga tgcagcgtgg aaaaatgccg ggcagggcta
    42301 cgagaaagtc aaaaagctcg atgcggagca caacaagtta atctggctga cgcccgcgcc
    42361 tgcaaataac acctggacta tcgccgtgcg tcaggatgtg gcagagaaaa acaaactcac
    42421 ttcgcttgcc gacctgagtc gttatctgca agagggcggc accttcaaac tggcagcctc
    42481 ggcagagttt atcgaacgcg ccgatgcgtt acccacgttt gaaaaagcct atggctttaa
    42541 gctcggtcag gatcagttgc tgtcactggc tggtggcgac acggcggtga cgattaaagc
    42601 cgctgcccag caaacctctg gcgttaatgc cgcaatggct tacggcactg acggtccggt
    42661 cgcggcgctg gggctgcaaa ccttaagcga tccgcaaggc gtgcaaccta tctacgcgcc
    42721 tgcaccagtg gtgcgtgaat cggtgttgaa ggagtatccg caaatggcac agtggctaca
    42781 gccagtcttc gccagcctcg atgcaaaaac attgcagcaa ctgaatgcca gcattgcagt
    42841 ggaaggactg gatgccaaaa aagtggctgc cgactacctg aaacaaaaag ggtgagcgaa
    42901 gtaatttccc gtgacttatc tacgtattaa tcctgttctg gcgctgctgt tgttgctgac
    42961 ggcaatcgca gcggcgctgc cgtttatcag ttacgcgcct aatcgtttag tctcgggtga
    43021 agggcttcat ctctggcaac tgtggccgca aacgatctgg atgctggtgg gcgttggttg
    43081 cgcctggctg acagcctgtt ttgtccccgc taaaaaaggc agcatttttg cactcattct
    43141 ggcgcaattc gtcttcgtat tgttggtgtg gggagctgga aaggcggcga cccaactggc
    43201 gcaaaatggc agtgcgctgg cgcgtaccag cctcggcaga ggtttctggc tggctgcggc
    43261 gctggcattg ctggcctgta gcgatgccat ccgccgaatc tccacgcatc cgctgtggcg
    43321 ctggttgttg catatgcaga ttgccattat tccgctgtgg ttgctgtact ccggcacgct
    43381 taacgatctc tcactaatga aagaatacgc caaccgtcag gatgtgtttg acgacgcgct
    43441 ggcacaacat ctgacgttgc tgtttggtgc ggtgctgcct gcgttagtga ttggtgtgcc
    43501 gttgggcatc tggtgctact tttccactgc gcggcagggg gcaatttttt ctctgctcaa
    43561 tgtcattcag accgtgcctt cggtggcgct ctttggcctg ttgattgcgc cgcttgccgc
    43621 gctggttacg gcctttccgt ggctggggaa gctcggcata gcaggaaccg gaatgacacc
    43681 cgcactgatt gcgctggtgc tctatgcctt gctgccgctg gtgcgaggcg tggtagtcgg
    43741 tttgaaccag atcccgcgcg atgtgctgga gagcgccaga gcgatgggga tgagcggggc
    43801 gcagcgtttc ctgcatgttc agttaccgct ggcgttaccg gtatttctgc gcagcctgcg
    43861 ggtggtgatg gtgcaaactg taggtatggc ggtgattgcg gcgttaatcg gcgcaggcgg
    43921 ttttggtgcg ctggttttcc aggggctgct aagcagcgcc attgatttag tgttgctggg
    43981 ggtgatcccg gtaattgttc tggcggtgct taccgacgcg ctgttcgatt tgcttatcgc
    44041 actgctgaag gtgaaacgta atgattgaat ttagccatgt cagcaaactg ttcggcgcac
    44101 aaaaagccgt taacgatctc aatctcaatt ttcaggaagg gagtttttcg gtgctgattg
    44161 gcacatctgg ctccggcaaa tccaccaccc tgaaaatgat taaccgcctg gtggagcatg
    44221 acagcggagt gatccgcttt gccggagaag aaattcgctc gctgccagta ctggagttgc
    44281 gccgccggat gggctatgcc attcaatcta ttggcctgtt cccccactgg agcgtggcgc
    44341 aaaacatcgc taccgtgccg caattacaaa aatggtcgcg ggcgcggatt gacgatcgta
    44401 tcgacgaatt aatggcgcta ctggggctgg agtcaaattt gcgtgagcgt tatccgcatc
    44461 agctttccgg tggtcagcag caacgtgtgg gagtggcgcg tgcactggct gccgatccgc
    44521 aagtcttact gatggatgaa ccttttggcg cactggaccc ggtaacgcgc ggcgcgttgc
    44581 aacaagagat gacgcgcatt caccgtttgc tggggcgcac tattgtgctg gtcactcatg
    44641 atattgatga ggcgctacgg ctggcagaac atctggtatt gatggatcac ggtgaagtgg
    44701 tgcagcaggg gaatccgctg acgatgctga ctcgtccggc gaatgatttt gtccgccagt
    44761 tttttggacg tagtgaactg ggggtgcacc tgctttcgtt acgtagcgtg gcggattacg
    44821 tgcgtcgcga agaacgggca gaaggtgagg cactggcaga agagatgacg ctacgcgatg
    44881 cgctctccct gtttgtcgcg cggggatgcg aggtgctgcc ggtggtgaac acgcagggcc
    44941 agccttgcgg cacgctgcat tttcaggatc tgctggtgga ggcgtaagcg tatgaagatg
    45001 ttgcgcgatc cgctgttctg gctcattgct ctttttgtgg cgctgatttt ctggctgcct
    45061 tacagccagc cgctgtttgc tgccttgttc ccacaactgc cacgacccgt ttatcagcaa
    45121 gaaagttttg cagctctggc actggctcat ttctggctgg tgggaatttc gagtttgttt
    45181 gcggtgatca ttggcacagg tgccggaatt gctgttactc gcccgtgggg cgcggaattt
    45241 cgcccactgg tggaaactat tgccgccgtt ggacagactt ttccgcctgt cgcagtgctg
    45301 gcgattgccg ttccggtgat cggctttggt ctgcaaccag cgattatcgc cttgatcctt
    45361 tacggtgtgc tgcccatcct gcaggtgaca cttgccgggc tgggagcgat tgatcccagc
    45421 gtgacagaag ttgcgaaagg tatgggaatg agtcgaggtc agcgactgcg taaggtcgag
    45481 ctaccgctgg cggctccggt gattctggcg ggcgtgcgaa cttcggtgat tatcaacatt
    45541 ggtacggcga cgatcgcctc aacggtaggg gccagtacgc tgggtacgcc catcatcatc
    45601 gggcttagcg gatttaatac cgcgtatgtg atccaggggg cgttactggt ggcactggcg
    45661 gcgatcatcg cagaccgcct gtttgaaagg ctggtgcagg cgcttagcca gcacgcaaaa
    45721 taaaggtata acctgcgagc atgacgccac caattccgcc taacgccata aacaggaaca
    45781 gggcgatgac cccaatttta gctatgcgca taatgcactc cttatgttaa cgaaaggatt
    45841 gtacagtaaa gcgcatttgt taacgaatca ttaaataccg agtgggaaaa tatcatggcc
    45901 ttgttcttgc caactggtga gttgctgctg ttgggcggag gttcgatttt caccgcacca
    45961 caccagcaat gtacggcctt cgaatagttc agggcgtagt tgattgagcg agtgggcgag
    46021 cgaggacatc aatgcgccat ccttgttgac tggcaatcca gccctccagc cacagacggg
    46081 tggtatcctg aatattccag ccaaccacca gcgcatcttt accctgtttt ttacgtgccg
    46141 aagccagaca aatggcgatg tagttgatca gtacgccgtc gaggatcgcc agcagcgcct
    46201 ggagagtcgg ttgttggcac tgaagccgtc ggcgcagagg aataaacaga tgtgtggtga
    46261 gtgtctgggc ggggtaatcc tgaccgcgct ctttgatcca cgttcgcagg ctatgtagat
    46321 tgccgctgaa ccgccccggg tttcctggag agtgttttat ctgtgaactc aggctgccag
    46381 atcatcgttt ccgatggaag cataataagc tttttctgct tctgccggag ggatgtggcc
    46441 cagcctttcc agcaatcgtc gattgttata ccagt
//
LOCUS       DAJTOW010000022        46392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTOW010000022 DAJTOW010000000
VERSION     DAJTOW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.022"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(89..164)
                     /locus_tag="OO244_001318"
     tRNA            complement(89..164)
                     /locus_tag="OO244_001318"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Lys,seq:ttt)
     gene            complement(329..1120)
                     /gene="cpoB"
                     /locus_tag="OO244_001319"
     CDS             complement(329..1120)
                     /gene="cpoB"
                     /locus_tag="OO244_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5663208.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1130..1651)
                     /gene="pal"
                     /locus_tag="OO244_001320"
     CDS             complement(1130..1651)
                     /gene="pal"
                     /locus_tag="OO244_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5663209.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1686..2978)
                     /gene="tolB"
                     /locus_tag="OO244_001321"
     CDS             complement(1686..2978)
                     /gene="tolB"
                     /locus_tag="OO244_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5663210.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3111..4352)
                     /gene="tolA"
                     /locus_tag="OO244_001322"
     CDS             complement(3111..4352)
                     /gene="tolA"
                     /locus_tag="OO244_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5663211.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4417..4845)
                     /gene="tolR"
                     /locus_tag="OO244_001323"
     CDS             complement(4417..4845)
                     /gene="tolR"
                     /locus_tag="OO244_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5663212.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4849..5541)
                     /gene="tolQ"
                     /locus_tag="OO244_001324"
     CDS             complement(4849..5541)
                     /gene="tolQ"
                     /locus_tag="OO244_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5663213.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5538..5942)
                     /gene="ybgC"
                     /locus_tag="OO244_001325"
     CDS             complement(5538..5942)
                     /gene="ybgC"
                     /locus_tag="OO244_001325"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5663214.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6092..6385)
                     /gene="ybgE"
                     /locus_tag="OO244_001326"
     CDS             complement(6092..6385)
                     /gene="ybgE"
                     /locus_tag="OO244_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5663215.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6385..6498)
                     /gene="cydX"
                     /locus_tag="OO244_001327"
     CDS             complement(6385..6498)
                     /gene="cydX"
                     /locus_tag="OO244_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5663216.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6513..7652)
                     /gene="cydB"
                     /locus_tag="OO244_001328"
     CDS             complement(6513..7652)
                     /gene="cydB"
                     /locus_tag="OO244_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5663217.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7668..9236)
                     /gene="cydA"
                     /locus_tag="OO244_001329"
     CDS             complement(7668..9236)
                     /gene="cydA"
                     /locus_tag="OO244_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5663218.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10086..12719)
                     /gene="mngB"
                     /locus_tag="OO244_001330"
     CDS             complement(10086..12719)
                     /gene="mngB"
                     /locus_tag="OO244_001330"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5663219.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12737..14653)
                     /gene="mngA"
                     /locus_tag="OO244_001331"
     CDS             complement(12737..14653)
                     /gene="mngA"
                     /locus_tag="OO244_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5663220.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGVPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14823..15545
                     /gene="mngR"
                     /locus_tag="OO244_001332"
     CDS             14823..15545
                     /gene="mngR"
                     /locus_tag="OO244_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5663221.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15648..16517)
                     /gene="sucD"
                     /locus_tag="OO244_001333"
     CDS             complement(15648..16517)
                     /gene="sucD"
                     /locus_tag="OO244_001333"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5663222.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16517..17683)
                     /gene="sucC"
                     /locus_tag="OO244_001334"
     CDS             complement(16517..17683)
                     /gene="sucC"
                     /locus_tag="OO244_001334"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5663223.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17777..18994)
                     /gene="odhB"
                     /locus_tag="OO244_001335"
     CDS             complement(17777..18994)
                     /gene="odhB"
                     /locus_tag="OO244_001335"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5663224.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19009..21810)
                     /gene="sucA"
                     /locus_tag="OO244_001336"
     CDS             complement(19009..21810)
                     /gene="sucA"
                     /locus_tag="OO244_001336"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5663225.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22111..22827)
                     /gene="sdhB"
                     /locus_tag="OO244_001337"
     CDS             complement(22111..22827)
                     /gene="sdhB"
                     /locus_tag="OO244_001337"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5663226.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22843..24609)
                     /gene="sdhA"
                     /locus_tag="OO244_001338"
     CDS             complement(22843..24609)
                     /gene="sdhA"
                     /locus_tag="OO244_001338"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5663227.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24609..24956)
                     /gene="sdhD"
                     /locus_tag="OO244_001339"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24609..24956)
                     /gene="sdhD"
                     /locus_tag="OO244_001339"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5663228.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24950..25354)
                     /gene="sdhC"
                     /locus_tag="OO244_001340"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24950..25354)
                     /gene="sdhC"
                     /locus_tag="OO244_001340"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5663229.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25735..25842
                     /locus_tag="OO244_001341"
     CDS             25735..25842
                     /locus_tag="OO244_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663230.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26048..27331
                     /gene="gltA"
                     /locus_tag="OO244_001342"
     CDS             26048..27331
                     /gene="gltA"
                     /locus_tag="OO244_001342"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5663231.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27722..28288
                     /locus_tag="OO244_001343"
     CDS             27722..28288
                     /locus_tag="OO244_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5663232.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28339..30798
                     /locus_tag="OO244_001344"
     CDS             28339..30798
                     /locus_tag="OO244_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5663233.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30813..31544
                     /locus_tag="OO244_001345"
     CDS             30813..31544
                     /locus_tag="OO244_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5663234.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31541..32646
                     /locus_tag="OO244_001346"
                     /pseudo
     CDS             31541..32646
                     /locus_tag="OO244_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32798..33844
                     /locus_tag="OO244_001347"
     CDS             32798..33844
                     /locus_tag="OO244_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5663235.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33841..34632)
                     /gene="nei"
                     /locus_tag="OO244_001348"
     CDS             complement(33841..34632)
                     /gene="nei"
                     /locus_tag="OO244_001348"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5663236.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34668..35402)
                     /gene="pxpA"
                     /locus_tag="OO244_001349"
     CDS             complement(34668..35402)
                     /gene="pxpA"
                     /locus_tag="OO244_001349"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5663237.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35392..36324)
                     /gene="pxpC"
                     /locus_tag="OO244_001350"
                     /pseudo
     CDS             complement(35392..36324)
                     /gene="pxpC"
                     /locus_tag="OO244_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36318..36974)
                     /gene="pxpB"
                     /locus_tag="OO244_001351"
     CDS             complement(36318..36974)
                     /gene="pxpB"
                     /locus_tag="OO244_001351"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5663238.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36997..37740)
                     /gene="ybgI"
                     /locus_tag="OO244_001352"
     CDS             complement(36997..37740)
                     /gene="ybgI"
                     /locus_tag="OO244_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5663239.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38011..39492
                     /gene="dtpD"
                     /locus_tag="OO244_001353"
     CDS             38011..39492
                     /gene="dtpD"
                     /locus_tag="OO244_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5663240.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39642..41060)
                     /gene="phrB"
                     /locus_tag="OO244_001354"
     CDS             complement(39642..41060)
                     /gene="phrB"
                     /locus_tag="OO244_001354"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5663241.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41057..41566)
                     /locus_tag="OO244_001355"
     CDS             complement(41057..41566)
                     /locus_tag="OO244_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5663242.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41695..41982
                     /locus_tag="OO244_001356"
     CDS             41695..41982
                     /locus_tag="OO244_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5663243.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42037..42417)
                     /locus_tag="OO244_001357"
     CDS             complement(42037..42417)
                     /locus_tag="OO244_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663244.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42420..45806)
                     /locus_tag="OO244_001358"
     CDS             complement(42420..45806)
                     /locus_tag="OO244_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5663245.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(45825..46028)
                     /locus_tag="OO244_001359"
                     /pseudo
     CDS             complement(45825..46028)
                     /locus_tag="OO244_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11160 a        12624 c        11195 g        11413 t
ORIGIN      
        1 aggatgactc gacttcgtct cgcccttcgg cgccgttgct gatgcaacgt tatccttcac
       61 gttttactac cgcttccacc ttagcgattg gtgggtcgtg caggattcga acctgcgacc
      121 aattgattaa aagtcaactg ctctaccaac tgagctaacg acccactttt tcgttgcttt
      181 cggtttgttt gataccccgt ggcaacggcg gcatatatta ctgatttcag acttgagcgc
      241 aacaaaaatt tcgatgcaga tcactcaact gcttatgatt cgcacgacac gaccagaaat
      301 aatgcgactt ctggtcgtgt gttatgcatt acatcgcgtt cagacgtttt tgtgcctgtt
      361 tagcgccatc agtaccaggg tatttactga taacctgctg gtatacggct ttcgcttttg
      421 cggtgtcacc tttgtcctgc atgatgacgc cgactttaaa catcgcatca gcagcctttg
      481 gtgacttcgg atagtttttc actaccgaag caaaatagta cgccgcatca tcttttttac
      541 ccttgttgta gtttaactga cccagccaat aattggcgtt tggcaggtaa gttgaatcag
      601 ggtaattttt gatgaaattc tgaaatgcca ccattgcgtc atcctggcgg gatttatcct
      661 gcaccagcgc aatagctgca ttgtaatccg tgtttgcatc gccgcttttc accggcgcgc
      721 cagcattcgc agtaccagca tcagctgtcg gcgtcgttga tgccgtcgca ccgctttgat
      781 cgctgctggt tgattgcgcc gctgcaccac cgctgctgag gctgtcgatc tgcaacagga
      841 tctgcttttg ccgctccacg acctgattca gttgatactg attttcctga atctgaccac
      901 gcagggaatc aatatcggat tgattatcag aaagttgttg ctggagttgg gttaaaagct
      961 ggctgtgagc attagaaata cgctcaagtt gagtgacgcg gtcttcgacc gagcctgagc
     1021 tgacactact gattggtgcc tgagcaaaag cggcccaggg ggccgctata ccaaccagta
     1081 acgacagact caatagttga tgtctgaagt tactgctcat gcaattctct tagtaaacca
     1141 gtaccgcacg acggtttttg gagtatgccg cttcgtcatg acccagtact gcaggttttt
     1201 ctttaccgta agaaacgatg gagatctggt ctgcagaaac gcctttaccc tgcaggtaca
     1261 tcttaacggc gttcgcacga cgttcaccca gggagatgtt gtattccgga gtaccacgtt
     1321 cgtccgcgtg accttctacg gtgactttgt aagacgggtt gctacgcagg aagtttgcat
     1381 gtgcatccag catttgagcg aagtcagaac ggatatcgta cttgtccaga tcgaagtaaa
     1441 cgatgttgtt ctgctgcagc tgttgcattt gcagacgagc ctgctcttcg gaagacatgt
     1501 tgccgttgcc gccgttcgca tccataccag tgccggcacc cagcatgcct tcgctgccgt
     1561 cattgctggc gttcttgttg gaagaacatg ccgcaattgc cataacaggc agagcaatca
     1621 tcagcccttt cagcactttg ttcagttgca tttcaatgat tcctttacta ttcaattaat
     1681 tattatcaca gatacggcga ccaggcaggg aatttgacct gtccatcagt tgccggaaga
     1741 cgcgctttga aacgcccatc tgtagaaacc aaattcagca cggatcccat cccctgagaa
     1801 gagctgtaga ttaccatagt gccgttaggt gccagacttg gcgtttcatc caggaacgtg
     1861 gacgacagaa cttgtacgcc tcccgttgcc agatcttgtt tggcaatgtg ctgctgccca
     1921 ccattggagc tgaccattac cataaattta ccgtcgctgc tgacatccgc atcctggttc
     1981 tgcgaacctt cccaggtaat acgttgtggc gcaccgccgt tgatattcac tttataaacc
     2041 tgcggacgac cggcctggtc agaagtaaat gccaggttct ggctatccgg gaaccaggtc
     2101 ggttcggtat tgttactgcg accatcagtc acctggcgga tctgaccaga agccaaatcc
     2161 attacgtaca ggttcagact accggttttc gacaaggcga atgccagttt gctgccgtct
     2221 ggcgagaatg caggtgcacc gttgtgacgc gggaatgaag ccacctgacg tacagcgcca
     2281 tttgccagcg tctgaataac cagcgcggaa cgaccgcttt cgaaggtcac ataagccagt
     2341 ttagaaccgt ctggtgacca cgccggagac atcagcggct gcggtgaacg gtgaacgaca
     2401 aactggttgt aaccgtcata gtcagatacg cgcagttcat acgggaactg accgccgttg
     2461 gtctgaacaa cgtaggcaat acgggtacgg aacgcacctt taatgccggt cagtttttca
     2521 aacacttcat cactggcggt atgaccagca taacgcagcc actgcttgtt cactttgtac
     2581 gagttctgag caagtacagt acccggtgcg ccgccagtgt caacaagttg ataagcaaca
     2641 ttgtaagaac catccggatt cggagtgact tgaccgacaa ctacagcgtc aatgcccagt
     2701 gcggaccatg cagctggttg tacttcctgc gcactacccg gctgctgtgg cagacgagcg
     2761 cgatctaacg gattaaattt accgctgtta cgcaagtcag cagcaacgat gccgccaata
     2821 tcttcaggtg ccgcaccagg ccccgcccac tggaaaggaa caacaccaat aggacgaccg
     2881 gaatctacac cgctgtcgat cacaatgcgg acttcagcat gcagaactga tgcccacagt
     2941 atgagaaaac caaatgctac tcgtaatgcc tgcttcatca tatctccctt atctggaccg
     3001 atggcccacg ataatttagc agaatgttaa caaactaaaa tacacaaaac taccagaacc
     3061 ccgtggcaac cggtgcctga tgttgaccgt ccgaacagtc aacatcgcga ttacggtttg
     3121 aagtccaatg gcgcgttttt gaacacttca tatactgcct ggcttggtgg tttcgggatc
     3181 ttcgcaagtt tagctgctgc caacgcagcc tgacaaagtg cgggatcgcc accttcaggt
     3241 ttgatatcca gtaacatacc atcgggtgcc agttttatgc gcagcgtaca ggttttgcct
     3301 gcataggacg atgcgtcata gaacttactt tcgatagcag atttaatctg cccggcatag
     3361 ttattgatat cggcccctga tgcgccattg tttttagtat taccactccc ggcaggcgaa
     3421 gcattgttcc ctttcgcccc tccccccgtt ttcggtgcat tcttaccaga gcttagctca
     3481 ccgaaaatat catctgcctc tgcggcagct tttgctgcag cggctttttc agctgctgct
     3541 tttgccgctg ccgctttttt gtctgcggcg gctttttctg ccgctgcttt cttatcagct
     3601 gctgccttat cagcagccgc tttttctgct gcttttttgt cggctgcagc tttctctgct
     3661 gccgctttct tatcagctgc agccttttca gcagccgctt tcttctcagc ttctgccttg
     3721 gctttttcag cagcttcagt tgccgctttc tttcttgctt cagctgcagc tgcttctgcc
     3781 gcttccgctt tcttcttcag tgccgcagcg gctacctcgg cttttttctg cgcttcggct
     3841 gcggctttgg cggcttctgc ttctgctttt ttcttcgcgt ctgcagccgc tttcttcgct
     3901 gcttcttccg cagctttagc atctgccgcc gctttcgctg ccgccacttc agcttgcttc
     3961 tgctttaact cggcctgttt tgcggcttct tcagcctgct ttttctgctc ctgagccgct
     4021 aaccgctctt tctcaagttg cttcaggcgt tcctgttcag ccgcttgttt ctcacgcagt
     4081 tcttcagcag cctgctgttc cttcatcttg cgctgttcat cagaacgctt cgcgcttgat
     4141 tcctggcttt gcatgcgttt gtactgctca actaccgcac ctgaatcaac catgacagcg
     4201 tcgatggacg aaccaccgcc gcctccggct gaagcttcta tattctcatc gaacgaactc
     4261 cagatcagcg ccgcaaataa gatgacatgc agcactgctg aaataattat cgcccgcttg
     4321 agcttgtcgt tttgttcggt tgcctttgac actctcggtt tccaaaaact gttcgcctgt
     4381 tacccgctct ctttcaagca agggaaacgc agatgtttag ataggctgcg tcattaaacc
     4441 aaccgatttc acacccgcac tatgtaacaa gttcagtgct ttaattattt catcgtaagg
     4501 cacatctttt gcgccaccga tcagaaagac cgttttcggg ttggccttga aacggctgga
     4561 cacttccgcc accacctgct ctggtggtaa acgctccagg cgatctttct caaccaccac
     4621 ggtgtactga ccaataccag acacttcaac aatcactggc ggattatcgt tactgctcac
     4681 cgcctgtgat tcagtagcgt ctggcagatc gacctccacg ctctgggtga tgatgggcgc
     4741 tgtcgccata aagatcagca acagcaccag cagtacgtcc agcaacggaa caatgttgat
     4801 ttcggacttg agatcgcgac gacctcgtcc acgcgctctg gccatggctt accccttgtt
     4861 gctctcgcta acggtaaacg cctggcggtg cagaatcgcg gtaaactctt ccataaagtt
     4921 gtcgtaattc agttccagtt tgtttacgcg ctggttgaga cggttataag ccattaccgc
     4981 cggaattgcg gcgaacagac caattgccgt cgcaatcaac gcttctgcga taccgggcgc
     5041 aaccatttgc agtgttgctt gttttaccgc cccgagggcg ataaaggcgt gcatgatccc
     5101 ccagaccgta ccaaacagac caatatacgg gctgatggag ccaaccgtac cgaggaacgg
     5161 aatgtgcgtt tccagatttt caagttcacg gttcatggag atacgcatag cacgcgacgc
     5221 cccttccact acggcttccg gcgcatggct attggcacga tgcaggcgca caaactcttt
     5281 gaacccgctg taaaagattt gttccgaacc agtcagatta tcccgtttcc cctgactctc
     5341 ttgatagagg cgagagagtt cgattccaga ccagaattta tcttcaaacg cttcggcttc
     5401 acgcgccgct gcgttaagaa tacgggttcg ctggataata atggcccagg atgcgattga
     5461 aaaaccaatc aaaatcaaca tgataagttt aaccagaagg ctagccttca ggaacaaatc
     5521 aaggatattc atgtcagtca ctgcttaaac tccgcgacaa tagacttggg aagcgcacga
     5581 ggcttcattt tgagtgggtc aacgcaaaca accagaacct ctgcttcatt cagcaaagta
     5641 ttctcggcgt tgacaatacg ttgcgtgaaa accaaagagg tgccacgcat tgatgttatt
     5701 tcagtctgta tttcgagcat atcgtcgagc cgcgcaggtg cgtaatattc caccgtcatt
     5761 ttacgtacca caaaggcaac gcgttcagcc atcagcgcct gctgactgaa gtgatgatga
     5821 cgcagcatct ctgtgcgtgc tctttcataa aaagcgacgt aactggcgtg gtacaccaca
     5881 ccaccggcat cggtatcttc atagtagacg cgaaccggcc atcgaaacag cgttgtattc
     5941 actttacatc ccggtaatgc aacaaaagtt agagctttta aacgctgcta ctatacgcgc
     6001 gcgattgagg tttgggaatg agggcaagtt aagggcgcgt aaaaatttat gggcctggag
     6061 cggcccataa ttttttaaag agaattctga tttaaaagaa gaagaaaatc agcccgacaa
     6121 tgagaacaat atcggcaagc aatgggcaaa aaatcccttg ccaaagaacc ttctgcggac
     6181 gaaagcccac gccgtgaatc acaccagcac agacggccca catcagcaat aaaccatgcc
     6241 agatttccag ttcactggtc ttcgcggcaa aacgagatgg gtcccaaaac atacatcctg
     6301 ctaacagaag cgccatcacg aaggaaagcg cccgcagggg gcgcttgtcc attaccgcat
     6361 acaaggtcgc gataatctta ctcatcagat gtcttcttga ccggctttgc ctgattcgac
     6421 gtgttcaagc gccagcgcgg tgattacccc aaacgaacag gcaagaagcg ttcccagaat
     6481 ccatgcgaaa taccacattt ttagctcctt acttagtaca gagagtgggt gttacgttca
     6541 atatcttctt tggtgatacg accgaacatt ttccagtaac accaggcggt gtagagcaga
     6601 atgatcggta ccagaaccac cgcaacccag gtcatgacgt taagcgtcag ctggctggaa
     6661 gttgcatccc acattgtcag acttgcgttc atcatggtgc tggacggcat cacaaacggg
     6721 aacattgcga taccggctgt caggatgatg caggccagcg tcagggagga gaacacaaac
     6781 gcccacgcgg ctttatccat acgtgcagtc aggatggtca gcagcagcag aaccacaccc
     6841 agtgccggaa tagcccacag aattggcgtg ttgttgaagt taaccagcca tgcgccagct
     6901 tcacgaacca cttctttatt cagtgggtta gaggctgcgt aatggtccat tgtcgatttc
     6961 acgacataac catcgatacc gtacatcacc catacgccag ccagtgcgaa acagaccagt
     7021 gtcaccagcg cagccacctg agccgttgca cgggtacgca ggtgcagttc gcccacggta
     7081 cgcatttgca gataggttgc gccctgagta atgatcatcc ctacgctcac cacgcctgcc
     7141 agcaggccga acgggttaag caactggaag aagttaccgg tgtagtacag acgcagatat
     7201 tcatcaacgt tgaacggtac gccctgcaac aggttaccga acgctacacc aattaccagc
     7261 ggcggaacga agctaccaat gaagatgccc cagtcccaca tgttacgcca gcgggtttct
     7321 tcaatcttgg agcggtagtc aaaaccgacc ggacggaaga acaaagacgc cagcacgagg
     7381 atcatcgcca catagaagcc ggagaacgca gcggcataga ccatcggcca ggcagcaaag
     7441 agtgcgccgc ccgcggtgat cagccaaacc tggttaccgt cccagtgtgg tgcaatggag
     7501 ttaatcataa ttcgacgctc ggtgtcgtta cgaccgagga aacgggtgag catgcccacc
     7561 cccatgtcga aaccgtcagt gactgcaaaa ccaatcagca gaacgccaac cagcagccac
     7621 cagataaaac gcaatacttc ataatcgatc atttgacgac tcctgtctta gcgtgccggc
     7681 tgagtagtcg tggaagactg ctcaaagtga tagcgaccgg ttttcaggct gcttgggccg
     7741 aggcgtgcaa acttgaacat taagaacaat tctgccacca ggaacagggt atacaggccg
     7801 caaatcagca ccattgagaa gatgagatcg cctgcggtca gtgacgaatt cgccacagct
     7861 gtcggcagca cttcaccgat agcccacggt tggcggccat attcagccac gaaccagccc
     7921 gcttctacag caatccacgg cagcggaata ccgtacagcg cggcgcgcag aagccatttt
     7981 ttctcgccaa tgcggttgcg gatgacactc cagaaagaga gcgcgatgat tgccagcagc
     8041 aggaagccac acgccaccat gatacggaac gcaaagtaca gcggcgctac acgcgggatg
     8101 gagtctttgg ttgcctgttg aatctgcgct tcagtcgcat cagccacgtt tggcgtatag
     8161 cgtttcagca gcagaccgta accgaggtct ttcttcatgc tattgaactg gtcacgaacc
     8221 gcctggtcgg tagaaccaga acgcagttgt tcgagcagag agtacgcctt catcccgtta
     8281 cgaatgcgtt cttcatgctg caccatcagc tctttcaggc cgataaccgg ggtatccacg
     8341 gaacgcgttg caatgatgcc cagtgcgtaa gggatctgaa tcgcaaattt gttcgtctcc
     8401 tcttcctgat caggaatgcc gaacaaagta aaggcagcag gcgcaggttg cgtttcccac
     8461 tcggcttcaa tagcagccag tttggttttc tgcacgtcgc ccatttcgta gccggattca
     8521 tcacccagaa caataacaga cagaacagca gccataccga agctggcagc gatagcaaag
     8581 gagcgtttag cgaaggcgaa gtcacgacct ttcagcatat accatgcgct gataccgagg
     8641 atgaacatcg cgccagtcac ataaccagac gctacagtgt gaacgaattt cacctgagca
     8701 accgggttaa gcaccagctc ggagaagctc accatctcca tacgcatagt ttcaaagttg
     8761 aaatcggacg cgattgggtt ttgcatccag ccgttcgcaa ccagaatcca cagtgcggac
     8821 aggtttgaac cgagcgccac cagccaggtg acacacatat gctgaacttt acccagacga
     8881 tcccaaccga agaagaacag acctacaaag gtggattcga ggaagaaggc catcagacct
     8941 tcgattgcca gcggcgcacc gaagatatcc cctacatagt gggaatagta agaccagtta
     9001 gtcccgaact ggaactccat ggtcagaccg gtagccacac ccagagcgaa gttgatacca
     9061 aacaacttgc cccagaactt ggtcatatct ttataaatct gtttgccgga gaggacgtag
     9121 accgtttcca taatggccag gaggaacgcc ataccgagcg tcagtggcac aaaaaggaag
     9181 tggtacatcg cggtcaaggc aaactgtaag cgcgacagtt cgactatatc taacatcatg
     9241 actccttgct catcgcatga agactccgag agtgaacccc gttagaaagg gtcacacgca
     9301 tgccccaata gaaatttgtt tgaacacttt catcatacca tgtgaatgca cacaaaaacg
     9361 gcgtaaagaa aggttgcaaa catgttaata aaaactcaaa ttgatcccac gtatatatta
     9421 cgccgcaaaa tccttacaat aaacaggttt ttgttgaagc aaatttgcat ttttcgacgg
     9481 tgatcaattt atagctaaat tgccgccttt cagccaattt gatcgagaac aatttatctc
     9541 tttttgatgc ccatttccaa gacttataca ttgataaata tcaatttcta caaattatgt
     9601 taatcatgtt tcaacaaggt aactttagtg atatttatgt ttaaaatgtg tgaattataa
     9661 gactgaaatt agtttcggtt ggcaattaaa gataaaaaga gacgaagcaa aaacagcacg
     9721 gttgacctta attctttttt tggtcactgg ctaatacgac gcagagtttc aggattatta
     9781 ataaaggcat aaatctcccc ccccgccgat attaacattt aattaactta taattatatt
     9841 ataattaact cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg
     9901 agagtgaaga taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
     9961 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    10021 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    10081 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    10141 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    10201 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    10261 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    10321 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    10381 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    10441 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    10501 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    10561 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    10621 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    10681 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    10741 ggcgcttctt tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    10801 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    10861 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    10921 ctgttgttat gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt
    10981 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    11041 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    11101 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
    11161 aacacccggt catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca
    11221 ttgagcgcaa tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca
    11281 atcacgttgc caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca
    11341 atctggttga tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt
    11401 tgccgatcga ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc
    11461 tgaccgcgat cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc
    11521 acttcttccc gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc
    11581 ggcatgttgt cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca
    11641 gccagttcga agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg
    11701 ctgtcgtggg catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg
    11761 tatccaaaac ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta
    11821 ttttcaatac gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc
    11881 acgcgcatat atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc
    11941 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa
    12001 atttcgcgca gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc
    12061 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
    12121 tcgaggcgtt tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc
    12181 aacggcatca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
    12241 ttatccgtac cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat
    12301 ccattgtaga tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt
    12361 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
    12421 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg
    12481 ccggcttcta ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct
    12541 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
    12601 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
    12661 taccactccc gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
    12721 gtcgtttcct ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg
    12781 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
    12841 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
    12901 agaaaatgcc agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
    12961 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
    13021 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
    13081 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
    13141 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
    13201 ccaggcagaa tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa
    13261 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
    13321 taagcgagtt attgatccag gcgacaggta cccccaccac aaacagcatc agactgcccg
    13381 ctcccaatgt accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc
    13441 taagacgcaa gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga
    13501 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
    13561 ctaacgccgg tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca
    13621 aaattccgag cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat
    13681 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc
    13741 cccccgcgac aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct
    13801 ccgttttgac gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac
    13861 gctcgagggt aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    13921 gcacagaaag cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg
    13981 caaaaataca ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt
    14041 tagcaccttg cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat
    14101 actccgcagc catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag
    14161 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    14221 gcaattcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc
    14281 gcgtggtcag cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg
    14341 ccagcaacac cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct
    14401 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    14461 cagccaaccc ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca
    14521 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa
    14581 tggcctcttc acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag
    14641 tcgtcaggtt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac
    14701 aaaacaatac cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta
    14761 ttgtttacag gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa
    14821 taatgggaca caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc
    14881 gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg
    14941 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg
    15001 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
    15061 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc
    15121 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc
    15181 aggatcgtgt gtggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
    15241 aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt
    15301 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
    15361 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
    15421 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
    15481 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
    15541 gataagcaca aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    15601 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    15661 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    15721 agagcagcga atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg
    15781 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta
    15841 acatgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    15901 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta
    15961 gagccaggga tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc
    16021 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    16081 cccggtttgt gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg
    16141 cctgggcagt tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc
    16201 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg
    16261 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa
    16321 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    16381 gtgccgcctt tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt
    16441 tctgagtgga aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta
    16501 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    16561 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    16621 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg
    16681 cgccgatgat accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca
    16741 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    16801 tggttgcgcc gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta
    16861 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    16921 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    16981 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt
    17041 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    17101 gcgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    17161 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    17221 ggccagtcag cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt
    17281 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt
    17341 ctacaacggc accgagatac agctctttag cgatatcggt cgccgcttca accagaatct
    17401 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt
    17461 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    17521 cgcggccacc agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag
    17581 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    17641 agcgggcaaa cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    17701 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    17761 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt
    17821 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    17881 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    17941 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    18001 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    18061 tcagcttgcc atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca
    18121 tgccgagggt atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg
    18181 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca
    18241 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    18301 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    18361 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    18421 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    18481 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    18541 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    18601 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    18661 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    18721 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    18781 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    18841 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    18901 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    18961 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    19021 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    19081 cggagaggcg gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    19141 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    19201 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    19261 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    19321 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    19381 atcaagctcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    19441 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg
    19501 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    19561 tacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    19621 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    19681 ccggccccat ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt
    19741 ggcgaagtca ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata
    19801 accatattca aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc
    19861 gccctgaccg ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg
    19921 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    19981 cggaatgcct tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    20041 cagtttctca ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    20101 catttcaact gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat
    20161 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    20221 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    20281 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    20341 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg
    20401 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc
    20461 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    20521 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    20581 atcagtacag tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg
    20641 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    20701 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    20761 cagcactttg ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat
    20821 aacgaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    20881 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc
    20941 gaggtgttct ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag
    21001 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt
    21061 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    21121 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc
    21181 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    21241 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc
    21301 ggcaccaatc gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat
    21361 ggtttctttg ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt
    21421 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    21481 atctagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    21541 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    21601 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    21661 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    21721 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    21781 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    21841 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    21901 ttgtctacgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    21961 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg
    22021 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    22081 aacgcttgcg cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt
    22141 gatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    22201 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    22261 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    22321 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    22381 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    22441 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    22501 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac
    22561 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    22621 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    22681 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    22741 ttcttccgct tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata
    22801 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    22861 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    22921 cgactcaggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    22981 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    23041 cgccgtttcc atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct
    23101 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    23161 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    23221 cagcgcttta cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag
    23281 gcgatccaga gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc
    23341 gatagactct tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga
    23401 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    23461 cagtcccgga acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac
    23521 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    23581 caccggatcg acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc
    23641 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    23701 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    23761 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    23821 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    23881 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    23941 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc
    24001 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    24061 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    24121 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    24181 gtgaccggta cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    24241 cgactgaccg ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag
    24301 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    24361 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    24421 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    24481 acgggtcggg aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag
    24541 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    24601 caatttcatc acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    24661 acgacaatca ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac
    24721 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    24781 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    24841 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    24901 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    24961 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    25021 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    25081 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    25141 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    25201 gccactgcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    25261 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    25321 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    25381 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    25441 gcttgcgggt cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag
    25501 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    25561 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    25621 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    25681 aatttgacaa tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt
    25741 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    25801 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    25861 acttgattcg ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct
    25921 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    25981 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    26041 accttaaatg gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact
    26101 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    26161 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    26221 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    26281 gaccgattct aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca
    26341 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    26401 tacccgtctg ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat
    26461 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg
    26521 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    26581 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    26641 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    26701 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    26761 cgtgcgtacc gctggctctt cgggtgcgaa cccgtttgcc tgtatcgcag caggtattgc
    26821 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    26881 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    26941 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    27001 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    27061 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    27121 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    27181 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    27241 aatgcacagt tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    27301 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    27361 acccgccgtt caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat
    27421 aaaatccact atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa
    27481 caaatagatg atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata
    27541 tagcgcaccc ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat
    27601 taattacttt tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga
    27661 caatcaaata agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt
    27721 tatgtttaaa ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc
    27781 tgtttatgcg gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc
    27841 accgtgtgaa atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac
    27901 cagccacatt aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa
    27961 ctgtgatctg gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac
    28021 ctttgatagc tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagtacggg
    28081 tgaagccacc ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg
    28141 caccgctact ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt
    28201 tgcctggatg gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa
    28261 tgcaacctac gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc
    28321 ataaaatatt tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc
    28381 ctggtcgtgg cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa
    28441 tcaatgcgcg accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt
    28501 gagtattttg ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac
    28561 tggcacaaaa atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt
    28621 ggcttaaaac ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc
    28681 tcccgacctg aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt
    28741 ccgcaagcct ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa
    28801 ggtgtcgccg gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat
    28861 ggcagcagca gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg
    28921 cgcttacgca gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca
    28981 ggcgaaatat cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc
    29041 ctcggcgaaa cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca
    29101 ctggcaagtg acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc
    29161 ggtattgcac agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag
    29221 aaaaaagtcc caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca
    29281 ctggatgtca aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca
    29341 tcgacgccct tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca
    29401 cggccctcca tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg
    29461 gggatgctgt caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat
    29521 tctgccgcaa tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc
    29581 acgtgggcca gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt
    29641 aattacagca aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc
    29701 tccgatcgtc attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat
    29761 gcgcaggacg aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac
    29821 ctcaatcttt acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc
    29881 gccaacatca cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct
    29941 acgtcattca atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg
    30001 atttcgctcc ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac
    30061 agcaccacac accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg
    30121 tctgccggac tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag
    30181 cacctgagtt cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt
    30241 tccgtcagca gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat
    30301 cgccgcagct ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt
    30361 ccggttcagg gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt
    30421 tccagttacc agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca
    30481 gttgcagaaa acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg
    30541 gcttcccgtt ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct
    30601 cccctcggag cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag
    30661 gaaggacatg cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt
    30721 gatagccagc attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg
    30781 attttacctt gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt
    30841 tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg
    30901 tgtttaacgc taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc
    30961 cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg
    31021 tcgccctgcc gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa
    31081 agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc
    31141 gtgaaattcc tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc
    31201 agagtcgtat caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg
    31261 tcgaactgca gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg
    31321 cgtattatct gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca
    31381 aaaccgtgat ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca
    31441 gccagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact
    31501 gcagcggaca atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt
    31561 gttgctcgtc atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta
    31621 ctttggttca tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc
    31681 aggcaatgcc aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg
    31741 gaatctgacg atattaaaat aagatctggg aatctgacga tattaaaatt cccgtctatt
    31801 gtgacaacaa taccaacggc aactttgaaa gcgaacatgt ctacgtatgg gtaaacccct
    31861 acccgggggt gcaggatcgt tattatcaac tcggagtaac ttacaatggc gttgattatg
    31921 acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg tatcgacagt aagaacattg
    31981 acatttatac ccctgatggg atggcagaac aaaatttgct cgggtgatcc cgctaatatt
    32041 cacatgtcgc gaacctttct cgctcgcatg cggttatacg tcaaaatacg agaaatgccg
    32101 ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg tgcaatttga cggtgccggt
    32161 agcgttaacg aagaccctac tgcccaaaac ctgaaatatc atattactgg tctggaaaac
    32221 attcgcgtgc tggattgcag cgtcaatttt tccatttcac cggaaacaca gatgattgat
    32281 tttggtaaat ttaatttgct ggatattcgt cgccacacaa tgtcgaaaac attcagtatt
    32341 aagacgacaa aatcacaaaa tgaccaatgc accgacggat ttaaagtcag ttcctccttt
    32401 tataccgaag aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag
    32461 ctacgtttgc tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc
    32521 gatttcacca gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt
    32581 gcaggtaccc ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat
    32641 aactgatttg ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg
    32701 attttcccct tttccttgat tgctgttatt ttttcctagc ccggctttct ttatgctgta
    32761 tcgatacacc aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat
    32821 gttatgtgtg ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc
    32881 gctattactt gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca
    32941 acttccccgc tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa
    33001 cctcactggt tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt
    33061 actggtgacg ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact
    33121 gcctggaaat accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat
    33181 ggcgcaagat tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct
    33241 gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg ctgggtgata acgctgaagc
    33301 ggttaaccag caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt
    33361 gctcgcagtc gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct
    33421 catcccgatg ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc
    33481 ggaatggctg ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga
    33541 taaacaaatc ttactgcggg cactacgccc gctaccgcaa atcctgctgt cgatttttgc
    33601 tctgctggct atttgtgcag gtatggcgtg gggactgacc cggtttatgc atattgattt
    33661 tatgactgcc tacctcgcca ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc
    33721 agggagcaat gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat
    33781 tttgctgacg gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc
    33841 ctagtgctgg cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat
    33901 gatgccgcca cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag
    33961 cgccccatga tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat
    34021 ctccagtaac gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg
    34081 atttccagtc aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc
    34141 cagaaacgcc tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggcgacaa
    34201 taaccgttcc tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg
    34261 ttgtaaaaac ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct
    34321 ataaagcaga atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg
    34381 cggctcttcg ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag
    34441 cgttaaaccg ttgggaaaat gagttaacaa cgccttacca cgcgtttcca catgggtaac
    34501 gtgttgaccg ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc
    34561 agttagtggt ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg
    34621 gccttcaggc atggtgatat ccttttttca ttctttatta agcggggtta tgctgcgaca
    34681 acaatcccct ttttggcaaa tgtagctcgt aatcggcggg cgaacgccag agcgtgctcg
    34741 ccgtcgccat gcaggcagac cgtttgcgcc gcgaccgttg cccattcgcc ggtgatactt
    34801 ttgactctgc cgtgttgcac catctccagc gtttgcgcca acgcctgttc ttcgttttca
    34861 atcaacgcgc ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg
    34921 gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt
    34981 cctgccagcc caacgagaac caatgccgga tcgcaagcgt ataccgctct ggcgatggcg
    35041 tctgccagtt gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga
    35101 cgcattacgc cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc
    35161 tgggcgtaaa cggtttccgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg
    35221 ggaaaactcg ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc
    35281 tgcatggttt gcgcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc
    35341 gttaatagct ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc
    35401 attgtgcagc cgccacgcta attgttcaaa ataacgttgc tgatcttgcc gcgctttcag
    35461 tgcctcttcc agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag
    35521 atggtacata tcagcctcaa tgatacaggc aatacgtgga taaccaccgg tggtctgtgc
    35581 gtcgttcatc aacacaatcg gctgcccgtt atgcggcacc tgcaccacgc ccggtaacaa
    35641 accgtgagat aacagttcgc gatcggtgat gcgttttaaa atttgcccct gtaaacgata
    35701 gcccattcgg ttactttgcg agctaagctg ccagggcgaa cgccagaatg catcctgcga
    35761 ggcgcgatcg aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag
    35821 cagctgttta acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtca
    35881 gtcaccatcc ttcagtaaac ggccttccag cccgccaatc cccactttga gatcggtgct
    35941 gcatgacccc attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat
    36001 cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca atcgccagcc
    36061 ggtccagacg gcgttatcat ccaaccgcgc ttcgcaacct gcacccgtca gggcaaacca
    36121 cccatcagtt tcgaactcaa ctgttaactg accgagcgtg atctccagcg cgggggcatt
    36181 ggcgtcatta cccaccagca ggttagcaat gcgcagcgca ggcatatcca gtgcgccaca
    36241 gtggctgata cccgactggc aaaaaccgtg acgaccgcca tcctgcacag tggtgtacat
    36301 gcccgcacga ataatcttca gcatattccc tccttctgcg gtacaaatcg cacgctgtct
    36361 cccggacgta ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga
    36421 ccaatcaact gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg
    36481 atcccgacag aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt
    36541 tgttccggca aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg
    36601 tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atacgccgcg
    36661 accaccgcca aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga
    36721 gactcaggtt ccagggcctc gctctcctcc caccagcgtt gcaaacgctc tatggcatcc
    36781 agcgccagcg actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct
    36841 tcaaccacat tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta
    36901 gccagcgtca ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa
    36961 cgcgctcgtt gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat
    37021 aaaggtcaca tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc
    37081 gcgttcagtg gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg
    37141 aatggtctgt tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct
    37201 atcgataaaa ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc
    37261 ggtatcgcca caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc
    37321 cattcccggc acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat
    37381 gaccgtgatc cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc
    37441 aagcggcaaa tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt
    37501 acgcttcatt ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat
    37561 gaccgcatcc gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc
    37621 ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc
    37681 actaatcgcc gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat
    37741 cttcgcgcct tccgtattct gatagctaca ccgttaacat agcgttacat cgcctgttcg
    37801 tcgatagcca atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt
    37861 ttatatcatc acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat
    37921 aatcagatct gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta
    37981 cgtgacgttg ccgtcaattg tggaattatt atgaataaac acgcatcaca accgcgcgct
    38041 atttactatg tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc
    38101 ctgctgattc tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta
    38161 tttagcgcct actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat
    38221 aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat
    38281 gtggtgctgg gtgccagtga gatccatccg tcattcctct atctgtccct ggcgattatc
    38341 gtctgcggct atggcctgtt taaatctaac gtaagctgtc tgctcggcga gctgtatgag
    38401 ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt atgcggcggg taacgtgggg
    38461 tctattatcg cacctatcgc ctgtggttac gcccaggaag agtacagctg ggcgatgggc
    38521 tttggcctgg cggcggttgg aatgatcgcg ggtctggtca ttttcttatg tggcaatcgt
    38581 catttcactc atacccgcgg cgttaacaaa aaagtactgc gtgcgacaaa ctttctcctg
    38641 ccgaactggg gatggctgct ggttctgctg gtggcaacgc ctgcactgat taccgtgctg
    38701 ttctggaaag agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta
    38761 ctggcaaaaa tttatcgcaa agcagaaaac cagaaacagc ggaaggagct ggggctgatt
    38821 gtgacgctca ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggtagctcg
    38881 attagccttt atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgacc
    38941 gcgatgttcc agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg
    39001 gtggttaaag aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt
    39061 ggccttggcc tgatgagcgc cggattctgc attctgacct taagcgcccg ctggtccgca
    39121 atgtatggtc actcttctct gccactgatg gtgttaggcc tcgcggtgat gggctttgcg
    39181 gaactgttta tcgacccggt tgccatgtcg caaattacgc gcattgaaat tccgggcgtg
    39241 accggcgtat taaccggcat ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg
    39301 ggcgtgattg ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac
    39361 tccatcaatg catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta
    39421 ggactggtac tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg
    39481 ctggagtcat aataggccag caccgatgcc tgatgcgacg cttgacgcgt cttatcaggc
    39541 ctacgtattt cctgcaattt attgaatttg cacaaatttg taggctggat taggcgttca
    39601 cgccgcatcc ggcatctggg ctcgattgcc tgacgcgtct gttatttccc cttccgcgcc
    39661 gcctcatacg ctgccaacgt ttgtactctc gcctctttgt gctcgactat cggttgcgga
    39721 taatccagct tcacacctgc tttctgcgcc cacttccacg gctcatgcac cgctttccct
    39781 ggtacatcgc gcagctccgg taaccatcga cgaataaact cgccctcacg gtcaaatttc
    39841 tcgccctgag ttatcggatt gaaaatacga aaatacggcg cagcatcagt acccgtagaa
    39901 gccgcccact gccagccacc gttattagct gccaaatcac catcaatcag ctgcgacatg
    39961 aaatatcgct cgccttcgcg ccagtcgatc aacaaatctt tcaccagaaa actggctgta
    40021 atcatccgta gccggttatg catccagcca gtgctgttaa gctgacgcat agcggcatca
    40081 ataatcgggt atcccgtttt gcctttctgc caggcctgta aatgtgcggg attgctttgc
    40141 cactgcacac gatccgtcca ggcaataaat ggacgatgtt tacacaacga ggggtaatac
    40201 gtcatcagat gacggtagaa ctcgcgccag atcagctcac taagccagac actaccggcc
    40261 ccaccgtcca gcgcctgcgg ctgttccgcc aacaagcgat gcaaacactg gcgaggcgat
    40321 aacccgcccg ttgccaagct ggcggacaaa cggctggtgc cttccactgc cggaaaatct
    40381 cgttgttgct catactctcc agcaccgttc tcgcaaaact ggcgtaattg cgcaatcgcc
    40441 gctttttctt ccaccgggaa atgcgcagta tcgaaagact gacgaggata attcagcgta
    40501 atggatggcg cgggctttat cgatccgcta ctacgaactt ttggtgcagc gacgcactcc
    40561 ggcatccctt cccgcagccg tttcagccag gcattcttaa aaggcgtaaa gactttgtac
    40621 atctcgtgat tgccggtcat caccgccccc ggcggcagga tcacgctgtc atcaaatcct
    40681 tcacacacca cgttacgcag cgctctttca acgtccacat cccgcgctcg ctcattcacc
    40741 tcatactgat agttataaaa aagatgagta acgctgtttt ccgcgcacac ctgtttaact
    40801 atttcgacac tggcgacaaa gtcatccact tcacggaaca ataaaggaat acctttttcc
    40861 gccagcgcga tttgtagccc attcagttga gcattgataa gttcagcctg acgcggcgac
    40921 atgttatgcg tctcccactt gcgtggtgta gcgatataca gcgccagcac gcgtgcagac
    40981 gaattgcggc aggcggcagc cagtgcgaga ttatcgtgca gacgtaaatc ctggcgaaac
    41041 caggccagat gggtagtcat aaaactcctg aatggcgcaa gttaatcaca cgcggccaaa
    41101 gttcgaaata acgctgccct gaaagccagg cgtcaggata aagcgccata tagtgtttga
    41161 tacgcatcag cggcgcaaga agtggttgct cgccacggcg ataactgtcg ataagcgcag
    41221 ccagctgctg gcgttctgtg gaatcaatat gcgggcgaaa ataaccctga acgtgcatca
    41281 aaacgttggt gtgatcgcgc gggttggcgg gatgagaaag caaaacaatt acgcgttggc
    41341 ggtactggtt gtaaaaatca tccagatttt gccactgatg aatatcagcc acaaacgggc
    41401 caagtttgcg gtactcgggc tgagaatggg ccagaaagac gagtttatag cggctgtgat
    41461 aatcgagaag cgccccgcgc gttaagccat gttctttcag tcgatgtaac tcatgaaggg
    41521 cgaagatacg gattagggtg ctgtcatcaa atcgttgtag attcattact gctcctgaac
    41581 ggtatacagg agtaagtgta ggcgttggta aatgatgttt gtgaagttaa tccgctagca
    41641 gggcaagatt acgaaagcgc gctccccgca agcaactaat caaggtagtt aatcatggct
    41701 gaacacgata ttacgccaga agtcaccatt tccaaaacac agcgccgcta taaagtcggc
    41761 tatgtcagag cccgccacca ggaccgcaaa accggtatag cccggtatta ttcacaacat
    41821 cccagtctgc accttaaggg cgactggctg aaagaggccg gatttgatac cggacggggt
    41881 gtcacggtga agatttcgga ggggggcctg accatcatcg ctgacagtga cgaggtgcag
    41941 gaactgcggg atgggatgtt tagtgtgctg aacgaaagtt aaatattaaa aaagctggga
    42001 agatttaaaa ccttcctagc tttttattct gtcttattat aatggatact catcatcttc
    42061 cgatgaattt ataaattgct ccaagtttac atgaagagta cttaacctag tttttaactc
    42121 attaagtgaa gcgataattt catattttcc aacataatca tatgatggat cgtattcatt
    42181 atctatgtca gaagctaaaa atgatgatat tcgtagttta aatttagctt catctttatt
    42241 acattcattg gcataaggtg aatgccctat catagcatat tcatctaatc taaaaggata
    42301 agatggtttt aattcatcga tcaatgaaat ttcaatccat gttctatatc caggcattaa
    42361 tggactaatt tctttgagca aaacctttcc acttaataat gtattgagat tgttcattat
    42421 tatgtctccc acgggaaaaa tatatgtcta acacttccac catgttgatg aacttgtaaa
    42481 tgtggtaaat caccatcagg atttcctggt ccatgacctg acactctata tgaaggtttt
    42541 gttacagaag gatcttttat atcatcccaa tgatatgaac ctgcttttgt tttattttca
    42601 gtaagatatt ccattaagtt cttagtgctg gggcctgact cacctgtcat atttttataa
    42661 ttatgaccta aatatctatc cataaacaat tgttcagcgt cacttctaga cctaacagta
    42721 actttggttt cactactatc taataaacga ttcgcttttt ggttactaac agcacatgga
    42781 gttaatccaa gtggatcagc atattttaga ggattaggtg cataactgta gagatttaaa
    42841 ccgcctctca accctatcgg gtcagttacc atgaaccgcc cgacatccgg atcatagtac
    42901 ctgtgcagat tgtagtgcag ccccgtttcc ctgtcgaggt actgtccctg gtagcgcagg
    42961 ttctgctcca ccccgccgtg gtccttctcc tgtatcgtgt taccccagac ctgatacccc
    43021 gtctcccaca cgattttccc gtcgctgtcc gtcatctctt ccggcgcgcc gttcacatcc
    43081 gtgtgaaaat acagtatccg gttcgggccc tctttgcccg ccttatccac ccgcgccagc
    43141 ggttcataac tcccctggtc cctgtagatg tacaggctgc ctgttccctc cggcctgctc
    43201 tcctgcgcca gccgcattcc gtcccataaa aattttgtcc ggttgtacgg cttaccgtca
    43261 gggctgatgt gctgtttttc cgtccgcctc cccagtgcat cgtacacata ccggtatgtc
    43321 ccccgcgctg tcgtcacttc caccatccgg tgttccgcat cataacggta actctgcgtt
    43381 ccgctccggc cctccttttc caccgcacgg ccaaattcgt cataacggta gtgcacacca
    43441 cggtaagaca tcagccggtt gaacggcacc acgctgtttt ctgccgtggc cctttccccc
    43501 gcgcgcctcc ccagcaggtt tcccgccgcg tccacctccg tttccggctg gtgccacacc
    43561 gggtgccact gcgcctgctc cacccgcccc agcgggtcgt gcgtctccgt gatatgcccc
    43621 tgcaggtcat acacatagcg ccatttgccg ccctgagggt cggtcatccc cagcaacagt
    43681 cgttcttcgt cgctccagcg gaatgtcgtc acctgtccgg cttcgtcctg gtacgccgtc
    43741 acctgattct gcgcatccca ctggtgataa cgtgtgcgcc ccagtccgtc cgtcacgcac
    43801 agcctgcgct gcgccaggtc ccacgccagc cggtactgtt caccgctgtc cgtgtggtgc
    43861 cccgtcaccc gccatgccgc gtcatccgga ccttcatacc agccccactg gtaataatac
    43921 ccttcgccgc ccggctggcg gtgcagcgtc atgcagtggt tttcgtcgta agcaaactca
    43981 cgcagcaggt gccccgtgct gtcgtacacg ccgttcaggt ggccggcctc gtcatagctg
    44041 taacgacgca gcagtaaagg tgcagtctca gggaaatgct gataaatctg tgtcacccgc
    44101 cggggatgcg ccgccagttc gtaatacaga cggatgcacg gacgctgctg ttcgccgctg
    44161 atttccacaa tgcggccccg gtcgtcgtag tacagattca gacagttgct gttacggtcg
    44221 cccagcattt tcagccgctg gcgctgcggg tggtgcgggt cagcctcaaa cagaaaacac
    44281 tgcccgtcat cgctgctgat aagccagtgc ccctgctcac cacgccttac cgccagccct
    44341 tcaccggggc tgtaaaaccc gccccccggc agtacggctt ccatatccaa gcgtcgcccc
    44401 atgcccgaca caaaggtcat gcagttttcg tctgcgctac ccggtgtgca cacgaggctg
    44461 acttcataca ttacactcca gccctgacca aacattcctt ccgtacgttc gtcacgactg
    44521 ctgtacacgc gctgccagtc cagcggaaag tggcccggca gactgaaatc gaagtcatcg
    44581 tcatcggcca ggtatttggc cccggtggcg gcattgaccg gatgcggaag ggcactggtg
    44641 gccttctgcg tcagcatgct catcccgaca ctggcagcaa aacaggcgat tttagaaagc
    44701 attttgcccg ggcgaagaaa catcagcgcc acggttgcca ccagggttat ctgcgacctg
    44761 ccgcttctga tatccctgac caccagtaac ggaccgccaa tgcgcacatc cggtgacaca
    44821 aacgccccgt tccccggctc attcaccacc ctggcctcgc aggtacagcg tgccccgctg
    44881 cgtgtcgccg gctggctgtt gatacgcaca tcccgcgcgc cttccgcgat atattccgct
    44941 gcgggcggct cgtcatcgcc tttgaacaga ttactgatgc tgccaataac cgcattcact
    45001 ccctgaaagg cggtttccag cataccgccc ggattcagca ggttaagggc cgttcccagg
    45061 gggtcatcca gaaaggcggc ttttttttgt gcgagaaact gcgtcagtga ggaaggatgt
    45121 ttatggcagt cgattttatc gtcaggggtc ggggtggatc ccggggccgc actggccaca
    45181 gtgggctgcc atagttccga cacaaaatgt gtcacgccat ccaccatttc agaacgccct
    45241 tcttccgtac tgagctggtc gtgcagagcc cgggacatca gcacaggccc tgtcaccggg
    45301 ttaaccgcca gggctatggc ccccgccatt tccagatatt ccccggccac atcccggaac
    45361 gttctggcat cctcctgacg ctgcgcttct tcctgttctg ccttcgcctg cgcttcctgc
    45421 gcctctatgt cctgccgcga tgccgcagtc gccgctgccc gcgcggcagg tttgctgttg
    45481 atccatgtgt caccagagcc ggtgaggatg taaccttcaa tctgaggcgg gaaaagcata
    45541 tttgcgaggg ctgaacaggc ttcgctgacc ttttctgtca cgccggtgta caccatcgcc
    45601 acacccgcca caaaaccggt cagcacgacg gccgtgcacc ccatcgtgat ccccactgac
    45661 gtcgccagta acgaggttgt gccgacaatg agggcatcaa taacgacatt tgcggccacc
    45721 tccagcaccg cccccagcac atctgccata aacgacgtat gcagcagggc atcaccctgt
    45781 cttgccgccc agaatgcctc ttccatgtcc cgttccctgt atgattaatt cgtcctgact
    45841 tcctgccgca tcctgaagcg cagattcttt ttaatttcag cccagcgcgt catatctgcc
    45901 ggggtcagcg gtgtggttct ggcgtaggtg aaggccatca tgacccgctc acccggcaat
    45961 acaaatgcca cctgacactg gtacacctgc tcgttacccc ggagaaactg gctctgtatt
    46021 tccaaaccgc ctcgttgaga taatattccg tcataatgta aatagtccgt tttaataaag
    46081 gaattattta ttgacaggtt ctgttttttt aatcatgaat gtaataccca tcaccgcttt
    46141 acagcacact ttatctttcc agccataatc actaataata ttaagcaatt agcaatattt
    46201 acaacgacca gagcggatac atagttatgt atttaacaat cccaatatat tcactatgca
    46261 aataaaacca ctagtaacag acacgctacc gcctccatat aaaaatggac accttataaa
    46321 attgtatctt gcatagaaaa agctgaaata tcggatgcgt tttaattatc aactataaat
    46381 ctataagctt tt
//
LOCUS       DAJTOW010000023        45891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTOW010000023 DAJTOW010000000
VERSION     DAJTOW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.023"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            114..2267
                     /locus_tag="OO244_001360"
     CDS             114..2267
                     /locus_tag="OO244_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5663246.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4051
                     /locus_tag="OO244_001361"
                     /pseudo
     CDS             2320..4051
                     /locus_tag="OO244_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4111..4602)
                     /gene="yajQ"
                     /locus_tag="OO244_001362"
     CDS             complement(4111..4602)
                     /gene="yajQ"
                     /locus_tag="OO244_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5663247.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4770..5681
                     /gene="panE"
                     /locus_tag="OO244_001363"
     CDS             4770..5681
                     /gene="panE"
                     /locus_tag="OO244_001363"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5663248.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5644..6234
                     /gene="yajL"
                     /locus_tag="OO244_001364"
     CDS             5644..6234
                     /gene="yajL"
                     /locus_tag="OO244_001364"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5663249.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6288..7736)
                     /gene="thiI"
                     /locus_tag="OO244_001365"
     CDS             complement(6288..7736)
                     /gene="thiI"
                     /locus_tag="OO244_001365"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5663250.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7942..8184
                     /gene="xseB"
                     /locus_tag="OO244_001366"
     CDS             7942..8184
                     /gene="xseB"
                     /locus_tag="OO244_001366"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5663251.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8184..9083
                     /gene="ispA"
                     /locus_tag="OO244_001367"
     CDS             8184..9083
                     /gene="ispA"
                     /locus_tag="OO244_001367"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5663252.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9108..10970
                     /gene="dxs"
                     /locus_tag="OO244_001368"
     CDS             9108..10970
                     /gene="dxs"
                     /locus_tag="OO244_001368"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5663253.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11025..11942
                     /gene="yajO"
                     /locus_tag="OO244_001369"
     CDS             11025..11942
                     /gene="yajO"
                     /locus_tag="OO244_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5663254.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTQPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12053..12571)
                     /gene="pgpA"
                     /locus_tag="OO244_001370"
     CDS             complement(12053..12571)
                     /gene="pgpA"
                     /locus_tag="OO244_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5663255.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12549..13526)
                     /gene="thiL"
                     /locus_tag="OO244_001371"
     CDS             complement(12549..13526)
                     /gene="thiL"
                     /locus_tag="OO244_001371"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5663256.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13604..14023)
                     /gene="nusB"
                     /locus_tag="OO244_001372"
     CDS             complement(13604..14023)
                     /gene="nusB"
                     /locus_tag="OO244_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5663257.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14043..14513)
                     /gene="ribE"
                     /locus_tag="OO244_001373"
     CDS             complement(14043..14513)
                     /gene="ribE"
                     /locus_tag="OO244_001373"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5663258.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14602..15705)
                     /gene="ribD"
                     /locus_tag="OO244_001374"
     CDS             complement(14602..15705)
                     /gene="ribD"
                     /locus_tag="OO244_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5663259.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15709..16158)
                     /gene="nrdR"
                     /locus_tag="OO244_001375"
     CDS             complement(15709..16158)
                     /gene="nrdR"
                     /locus_tag="OO244_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5663260.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16309..16848
                     /locus_tag="OO244_001376"
     CDS             16309..16848
                     /locus_tag="OO244_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5663261.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17146..18030
                     /gene="tsx"
                     /locus_tag="OO244_001377"
     CDS             17146..18030
                     /gene="tsx"
                     /locus_tag="OO244_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5663262.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18207..18554)
                     /locus_tag="OO244_001378"
     CDS             complement(18207..18554)
                     /locus_tag="OO244_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5663263.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18683..19654)
                     /gene="secF"
                     /locus_tag="OO244_001379"
     CDS             complement(18683..19654)
                     /gene="secF"
                     /locus_tag="OO244_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5663264.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19665..21512)
                     /gene="secD"
                     /locus_tag="OO244_001380"
     CDS             complement(19665..21512)
                     /gene="secD"
                     /locus_tag="OO244_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5663265.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21540..21872)
                     /gene="yajC"
                     /locus_tag="OO244_001381"
     CDS             complement(21540..21872)
                     /gene="yajC"
                     /locus_tag="OO244_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5663266.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21895..23022)
                     /gene="tgt"
                     /locus_tag="OO244_001382"
     CDS             complement(21895..23022)
                     /gene="tgt"
                     /locus_tag="OO244_001382"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5663267.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23077..24147)
                     /gene="queA"
                     /locus_tag="OO244_001383"
     CDS             complement(23077..24147)
                     /gene="queA"
                     /locus_tag="OO244_001383"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5663268.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24240..24813
                     /gene="acpH"
                     /locus_tag="OO244_001384"
                     /pseudo
     CDS             24240..24813
                     /gene="acpH"
                     /locus_tag="OO244_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24818..26635)
                     /gene="malZ"
                     /locus_tag="OO244_001385"
     CDS             complement(24818..26635)
                     /gene="malZ"
                     /locus_tag="OO244_001385"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5663269.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26791..28164)
                     /gene="proY"
                     /locus_tag="OO244_001386"
     CDS             complement(26791..28164)
                     /gene="proY"
                     /locus_tag="OO244_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5663270.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28240..29559)
                     /gene="brnQ"
                     /locus_tag="OO244_001387"
     CDS             complement(28240..29559)
                     /gene="brnQ"
                     /locus_tag="OO244_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5663271.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29966..31261)
                     /gene="phoR"
                     /locus_tag="OO244_001388"
     CDS             complement(29966..31261)
                     /gene="phoR"
                     /locus_tag="OO244_001388"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5663272.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31319..32008)
                     /gene="phoB"
                     /locus_tag="OO244_001389"
     CDS             complement(31319..32008)
                     /gene="phoB"
                     /locus_tag="OO244_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5663273.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32198..33400
                     /gene="sbcD"
                     /locus_tag="OO244_001390"
     CDS             32198..33400
                     /gene="sbcD"
                     /locus_tag="OO244_001390"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5663274.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33397..36540
                     /gene="sbcC"
                     /locus_tag="OO244_001391"
     CDS             33397..36540
                     /gene="sbcC"
                     /locus_tag="OO244_001391"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5663275.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36666..37847
                     /gene="araJ"
                     /locus_tag="OO244_001392"
     CDS             36666..37847
                     /gene="araJ"
                     /locus_tag="OO244_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5663276.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37990..38898)
                     /gene="mak"
                     /locus_tag="OO244_001393"
     CDS             complement(37990..38898)
                     /gene="mak"
                     /locus_tag="OO244_001393"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5663277.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39023..39934
                     /gene="rdgC"
                     /locus_tag="OO244_001394"
     CDS             39023..39934
                     /gene="rdgC"
                     /locus_tag="OO244_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5663278.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40012..40467)
                     /locus_tag="OO244_001395"
     CDS             complement(40012..40467)
                     /locus_tag="OO244_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663279.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40464..40799)
                     /locus_tag="OO244_001396"
                     /pseudo
     CDS             complement(40464..40799)
                     /locus_tag="OO244_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40954..41238)
                     /gene="ppnP"
                     /locus_tag="OO244_001397"
     CDS             complement(40954..41238)
                     /gene="ppnP"
                     /locus_tag="OO244_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5663280.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41310..41987)
                     /locus_tag="OO244_001398"
     CDS             complement(41310..41987)
                     /locus_tag="OO244_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5663281.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42245..42436)
                     /gene="yaiA"
                     /locus_tag="OO244_001399"
     CDS             complement(42245..42436)
                     /gene="yaiA"
                     /locus_tag="OO244_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5663282.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42486..43010)
                     /gene="aroL"
                     /locus_tag="OO244_001400"
     CDS             complement(42486..43010)
                     /gene="aroL"
                     /locus_tag="OO244_001400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5663283.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43193..43651)
                     /locus_tag="OO244_001401"
     CDS             complement(43193..43651)
                     /locus_tag="OO244_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5663284.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43771..44580
                     /gene="proC"
                     /locus_tag="OO244_001402"
     CDS             43771..44580
                     /gene="proC"
                     /locus_tag="OO244_001402"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5663285.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44597..45712)
                     /gene="adrA"
                     /locus_tag="OO244_001403"
                     /gene_synonym="dgcC"
     CDS             complement(44597..45712)
                     /gene="adrA"
                     /locus_tag="OO244_001403"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5663286.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11935 a        11969 c        11224 g        10763 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttataat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aaatgcagaa tgcatggcac acattacctt tacgcttaat aaattaacaa cacaaccagc
     3901 aataacaaaa atagatagcg tgggtaatga gcaactttat cagattggca aaatctacca
     3961 taagttggaa attaaacctg agccagagca acaggtttta attcagtcgc tttatgaccg
     4021 ggcatcagga agcgcctctg caactcaata aatgcatggt caaaagggcc accacggtgg
     4081 ccctgacaat cagtagctat atccagaaag ttaatcgcgg aagtttttga actggaacgg
     4141 ctgaccgaga tcgccaccgc gcaccatcgc catgacagcc tgcaaatcat cacgagattt
     4201 gccagtcacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
     4261 gatcatcttg acgatttttt tctgagtggc gctctcaatg ccctgtttca gtttcgcttc
     4321 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc
     4381 aatgccgcgc ttcagcagct tggcacgcag aatatccagc aactgattga cctggaagtc
     4441 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
     4501 gttacggaag tcaaaacggg actccacttc gcgactcgcg ttatcgaccg cgttacgtgc
     4561 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
     4621 ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt
     4681 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
     4741 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
     4801 tagggcaatt atggcttaca gcactttgta aacagggtca tgaagttcag ggctggctgc
     4861 gtgtaccgca accttattgt agtgtgaatc tggttgagac agatggttcg atatttaacg
     4921 aatcgctgac cgccaacgat cccgattttc tcgccaccag cgatctgctc ctggtgacgc
     4981 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc atccacactg cctgtaacta
     5041 cgccaatact gttaattcac aacggcatgg gcaccatcga agaattgcaa aacattcagc
     5101 agccattact gatgggcacc accacccatg ccgcccgccg cgacggcaat gtcattattc
     5161 atgtggcaaa cggaatcacg catatcggtc cggcacggca acaggatggc gattacagtt
     5221 atctggcgga tattttgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
     5281 ccgagctgtg gcgcaagctg gccgtcaact gcgtgattaa tccactgact gccatctgga
     5341 attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag
     5401 tcgcggcagt gatcgaacgc gaagggcatc atacttcagc ggaagatttg cgtgattacg
     5461 tgatgcaggt gattgatgcc acagcagaaa atatctcgtc gatgttgcag gatatccgcg
     5521 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
     5581 atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
     5641 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
     5701 accacgatca acctcttggt tcgcggcggt atcaaagtca ccactgccag cgtcgacagc
     5761 gatggcaacc tggcaattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
     5821 gtcgaagtgg ctgatggcga atatgacgtg atcgtgctgc ctggtggcat taaaggcacg
     5881 gagtgttttc gcgatagcac tctgctggtt gaaaccgtta aacagttcca ccgttccggg
     5941 cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt
     6001 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg
     6061 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt
     6121 acagctatcg actttggtct gaaaattatc gacctgctgg ttgggcgcga gaaagcccat
     6181 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg
     6241 atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat
     6301 accttcacat tgttaaagcc ttgctcgcgc agatagagcg cctgcagacg gctcatcacc
     6361 ccgcgctcac accacagcag ccaggttttg ttctggtcga gatcgccaaa tttggtgctc
     6421 agtttataga acggcagaga aaccacatca atcccttcga ctttcagtgg cttatcttcc
     6481 tgttcatcga cagaacggat atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt
     6541 tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc
     6601 gcttcctcaa ccactttatc gagaatactg aagtcgaact tctcttcttc cgcttcaatc
     6661 ttcgatttaa ccgctttcac cgtcgggctt ttggagatca cgccacaata ttccggcatc
     6721 gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg
     6781 taagaaatca gcggacgcag gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg
     6841 gtcagcgtct gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg
     6901 taacgttcag ccactttaga tgcggcacgc accatcatac gtttgaggat aacgcccatc
     6961 tgaccgtcgt cgattttctc gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca
     7021 aaacgcacgc ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgcacg
     7081 ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga
     7141 cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagcaca
     7201 tcttcctggg tgccaatcgg gaaaccacca ataccttcgt agcgaccttt aatcagcagg
     7261 aggcgatcgt cttccacttc cagatgaaca gttacatccg ggttagtcag cttcacgcgc
     7321 gcggattcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
     7381 aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg
     7441 cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg
     7501 acttcgagaa tatggtggat acccggaata cgggtcaggg cgtcgcgaat agtcagacgc
     7561 tggttttcat ctttcgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
     7621 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
     7681 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
     7741 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
     7801 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
     7861 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
     7921 attgagagtc agacattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag
     7981 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
     8041 gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta
     8101 caacaagccg aacagcgcgt acaaattctg ctgtctgaca atgaagacgc ctctctaacc
     8161 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
     8221 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
     8281 tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct ttcctggttt
     8341 atgccaccgg tcatatgttt ggcgttagca caaacacgct ggacgcaccc gctgctgccg
     8401 tagagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
     8461 atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcaaac gcgattctcg
     8521 ctggcgacgc tttacaaacg ctggcgttct cgattctaag cgatgccaat atgccggaag
     8581 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
     8641 gaatgtgcgg tggtcaggca ttagatttag acgcggaagg caaacacgta cctctggacg
     8701 cgcttgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg
     8761 gtgcattaag cgccggagat aaagggcgtc gtgctctgcc agtactcgac aagtacgcag
     8821 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggta ggagatactg
     8881 caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac
     8941 ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgtcagg
     9001 cgctgaaaca actggctgaa cagtcactcg atacctcggc actggaagcg ctagcggact
     9061 acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata
     9121 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccga
     9181 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
     9241 gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact
     9301 atgtctacaa caccccattt gaccaattga tttgggatgt gggacatcag gcttatccgc
     9361 ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc
     9421 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa
     9481 cctccatcag tgccggaatt ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc
     9541 gcaccgtctg tgtgattggc gacggcgcga ttaccgctgg catggcgttt gaagcgatga
     9601 atcacgcggg cgatattcgt cctgatatgc tggtggtcct caacgacaat gaaatgtcga
     9661 tttccgaaaa tgttggcgcg cttaacaacc atctggctca gctgctttcc ggtaagcttt
     9721 actcttcact gcgcgaaggc gggaaaaaag ttttctctgg cgtgccgccc attaaagagc
     9781 tgctcaaacg taccgaagaa catattaaag gcatggtggt gcctggcacg ttgtttgaag
     9841 agctgggatt taactacatc ggcccggtgg acggtcacga tgtgctgggg cttatcacca
     9901 cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag
     9961 gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg
    10021 atccctccag cggttgtttg ccgaaaagta gcggcggttt gccgagctat tcaaaaatct
    10081 ttggcgactg gttgtgcgaa acggcagcga aagacaacaa gctgatggcg attactccgg
    10141 cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat cgttacttcg
    10201 acgtggctat tgccgagcaa cacgcagtga cctttgccgc cggtctggcg attggtggat
    10261 acaaacccat tgtcgcgatc tactccacct tcctgcaacg cgcctatgat caggtgctgc
    10321 atgacgtggc aattcaaaag ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg
    10381 gtgctgacgg tcaaactcac cagggcgctt ttgatctctc ttacctgcgc tgcataccag
    10441 aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct
    10501 atcactataa cgatggcccg tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg
    10561 aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga
    10621 aactggtgat ccttaacttt ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc
    10681 tgaacgctac gctggtcgat atgcgttttg tgaaaccact tgatgaagcg ttaattctgg
    10741 aaatggccgc cagccatgaa gcgctggtca ccgtagaaga aaacgccatt atgggcggcg
    10801 caggtagcgg agtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca
    10861 ttggcctgcc ggacttcttt attccgcaag gaactcagga agaaatgcgc gccgaactcg
    10921 gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg gctggcataa tccctactcc
    10981 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
    11041 taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
    11101 cagatcgcgg taatcacgca tggacacagc cggaagagag cagccgtccc ataattaaac
    11161 gcgcgctgga aggtggcata aatttctttg ataccgccaa tagctattcc gatggtagca
    11221 gcgaagagat cgttggtcgc gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg
    11281 ccaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
    11341 tgcgctctat caacgacagc ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa
    11401 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctg aacgacgtgg
    11461 taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc
    11521 aggcgctgga actacaaaaa cagcactgct gggcgcagtt tgtcagtatg caggatcact
    11581 ataatctgat ttatcgcgaa gaagagcgcg agatgctgcc tctgtgttat caggagggcg
    11641 tggcggtgat tccgtggagc ccgctggcgc gggggcgact gacgcgtcca tggggagaaa
    11701 ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa
    11761 atgacgcgca gatcgcagag cggttaacgg gcgtcagtga agaactcggt gcaacacgag
    11821 cacaagttgc gctggcctgg ttgttaagta aaccgggcat tgccgcaccg attatcggaa
    11881 cttcgcggga agaacagctt gatgagctgc tgaacgcggt ggatatcact ttgaagccat
    11941 aacagattgc cgagctggaa acgccgtata aaccgcatgc ggtcgtagga tttaaataag
    12001 agtccaggcc tgatgagacg tgacaagcgt cacatcaggc atcggtgcac aactacgaca
    12061 gaatacccag cggccagtga tgaccgataa agtacaggat gcctgcggaa atcaccccgg
    12121 cgacaatatc gtcgatcatg atccccatgc cgccatgtac attgcgatca aaccagcgga
    12181 tcggccacgg cttccacata tccagaatac ggaaaatcac aaacccggcg gcaacccact
    12241 gccagtcatt ggtcggcagc gccatgagcg tgatccacat accaataaat tcgtcccaga
    12301 caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt ttgatgacaa agatagacgc
    12361 cgatacagat ccccaacatc accaccagcg agtagagctg ccagggtaaa aaggtcatca
    12421 gataccagaa agggatcgcc gccagcgatc ccatcgagcc aggaacgatt gggcttaatc
    12481 cacttccgaa tccgacagca agcagatgcc acggattact catcttcagg cgacttttcg
    12541 cgacatcttt atggcgtgac aaaatggtca tatcctttcc agtcaaacgt gacaggttcg
    12601 ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac
    12661 ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac ggttcagttc cggcaccgtg
    12721 aaacacaact cgtaatcttc accgccagag agcgcccagc gcagcgcctg ttccggttca
    12781 acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag
    12841 tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct
    12901 gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgatgg acggagatga
    12961 cgtttgatca agtagtcagc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc
    13021 gccagcccgg cggcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc
    13081 gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt
    13141 gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga
    13201 tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat
    13261 gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc
    13321 agtgctttat aagccagatc agcaggatcg atatcaggga ggaaatggtt gcccgccacc
    13381 agcgtatcag tgctgatcgc cagggtctgt ttctcgggga tattgagaag agcgcaatcg
    13441 tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca
    13501 atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc
    13561 ggcgatcatg gaacagtctt ccgtgaatct accggcctgg atattacttt ttgttagggc
    13621 gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc
    13681 cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcactac
    13741 gtttagacag ttcgtacagc gcaatgcgca gtactgcttt ttctacctgg cccagttctt
    13801 ccagcaggcg ggacaggtag ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca
    13861 ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca
    13921 ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct
    13981 ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa
    14041 tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct
    14101 gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg
    14161 gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg
    14221 cttgcaccac cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc
    14281 gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca
    14341 taggcaccag gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc
    14401 agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg
    14461 gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc
    14521 cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg
    14581 cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac
    14641 atgacgtatc tctttgaatt taaattgggg ggcgtcggct aatttctcaa gccctggcag
    14701 cgagcataat ccacgggcgt cgctgcctaa tagtttaggt gcgatataga caatcagctc
    14761 atcgactaaa cccgcctgta gcaatacgcc agcgagcgtt ggccccgctt ccacccagat
    14821 gctgttaatc tgctgtttac ccagttgcat catcagtaca accagatcca gatgaccttt
    14881 atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat cttcctgcgt
    14941 acgcgcgaac caggtttcgc cgggctgctg cacaatgcga tgttccggcg tcacgcgatt
    15001 ttggctatca atcacaatac gtactggctg gcggagattt tgttgcgggt agagcgcctg
    15061 agtttgttca tccagttcag accaacgcac tgttaaggct ggatcatccg ccagcaccgt
    15121 ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc
    15181 ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc gccgtgcggc catcaagcga
    15241 tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgcttga gaaagccttt
    15301 attcaattgc tcggcttcac tcatcatcag cccgtggctg acgtcaatgc cagcctgttg
    15361 cagacggtaa agtccacgcc cagcgacctg cgggttagga tcttgcatcg ctgcgaccac
    15421 gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt ggcgtacggc catgatggct
    15481 acagggttcg agtgtcacat acgccgttgc gcctttggct ttttcacccg ccatacgcaa
    15541 cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg taaccttcac cgacaatttc
    15601 gccatctttg acaatgacgc acccgacatt cgggttgggg tgggtggtaa aacgtcctcg
    15661 ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag
    15721 gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa
    15781 acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat
    15841 cttgctcggt acttcgcgct caccggtggc gcgcagctgc gatttaatat gattgattgc
    15901 catttcaacg taatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg
    15961 caatttctct tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac
    16021 cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg
    16081 gcgtacggat gaaccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa
    16141 acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacggaaatc tattttaccc
    16201 tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcgctg
    16261 cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac
    16321 gtttttcgac tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt
    16381 gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aggagatgac gcaactcaat
    16441 caagaaacag tcaaaatcac tcagcaaaac aggctgaatg caaaatccag cagtggggtt
    16501 taccttctgc ctggagcgaa aacaccggca agactggaaa gccagatcgg tactttacgt
    16561 atgtcgctgg tgaatattac gcctgataca gatggcacca cccttacact acgtattcag
    16621 ggtgagtcga atgatccctt gcccgcattc agcggaactg ttgaatatgg gcaaattcag
    16681 ggaacaatag acaactttca ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc
    16741 agcgtcctcg cccccagcga tgttgatatt ccgttacaat taaaggggat ttctgtggat
    16801 cagttaggct ttgtacgtat ccacgacatt caacctgtta tgcagtaaac gtatttcggg
    16861 acgattttgt gcgtcccgca acatcttccc cgtcattttg ttactctgct tacatcacct
    16921 ggattgatag taaaagtttg caacaagggc gaaagtcagt acaatccccg cccgaatgtg
    16981 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    17041 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    17101 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    17161 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    17221 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    17281 cacacccgtt tcggaccgca gatccgcaac gatacctacc ttgagtacga agcattcgct
    17341 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    17401 tccgatgcta aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccacgt
    17461 ttctccatcg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    17521 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggtcgcca gagcacctgg
    17581 tacatgggtc tgggtaccga tatcgacact ggcctgccga tgagcctgtc catgaacgtc
    17641 tatgcgaaat accagtggca gaactatggc gcagcgaacg aaaacgagtg ggacggttac
    17701 cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt ggggcggtca gctgagctac
    17761 atcggcttca ccaacttcga ctggggttcc gatttagggg atgacagcgg taacgcaatc
    17821 aacggtatta agacccgtac taataactct atcgcttcca gccatattct ggctctgaac
    17881 tacgatcact ggcactactc tgtcgtagct cgttactggc acgacggtgg tcagtggaac
    17941 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    18001 ggttacctgg tagtaggtta caacttctga ttatgaaaat gccgggattt attcccggca
    18061 tttctgattg ttaacaacgt gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc
    18121 ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata acgtaggcct gataagcgta
    18181 gcgcatcagg caattttacg ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca
    18241 gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg
    18301 gtcgtaccat actgatccgc ttcggtatat ttcgaatgct catgatcgtg gcaatagaga
    18361 cacaacaatt cccagttact gccatcttcc gggttattgg tatggtcgtg atcaatgtgg
    18421 tgaacggtaa gttcacgcag gttagaataa acaaactcgc gggaacagcg accgcagacc
    18481 cacggataga tttttaatgc tttttcgcga tagccgcttt ctaaccgtgc atagtttttt
    18541 gggatgatag ccatgaaaag ttacctggat gaaatgtagg gttacgcgca gtttcccaag
    18601 atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct gaccgggatt
    18661 ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc ccttcttttt
    18721 ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc aacgcagatg
    18781 ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc agcgagaagc
    18841 cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag gtagtaccgg
    18901 atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg
    18961 taccgcgacg gatcttgcgg aagttttcac gaattcggtc cgataccacg atactgtcgt
    19021 taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca atctcgatat
    19081 ggaataacga caaaataccc agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc
    19141 ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac agcagcgctg
    19201 ccatcaacgc catcgcaccg gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact
    19261 caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc agaacttggc
    19321 tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc atgatgtcgt
    19381 ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc aatgcatcac
    19441 gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta ccaccggtga
    19501 aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg attaacagca
    19561 gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact ttacggccgt
    19621 ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat tgacagcttc
    19681 ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac aatcgcggta
    19741 aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat tgccccggta
    19801 cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc gaagatagaa
    19861 ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac
    19921 tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc aagggttaag
    19981 acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga cataataccg
    20041 acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa cttcttatag
    20101 aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc cagcccctgc
    20161 tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc
    20221 gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt cgggttgttg
    20281 atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat gttaatcacc
    20341 tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct gtctttgtac
    20401 tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt agacataatg
    20461 ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc gtcctggctg
    20521 gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag cacaaccggc
    20581 tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga tgccgcagcg
    20641 gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc gcccagaatc
    20701 tctttcgcac gcgcagtgtc ctgaataccg ggcagttcaa caacgatacg gtcagcaccc
    20761 tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg caggatatta
    20821 atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct cattaccgca
    20881 cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt gctcagatac
    20941 gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc gtagttgttt
    21001 tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg taggctatcg
    21061 atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat caggaagtga
    21121 acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc tgccagccag
    21181 cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat aacgcccatt
    21241 aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc
    21301 tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt ctggacctgg
    21361 atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac agccggatcc
    21421 tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac gatcagcatg
    21481 acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa aaattttaat
    21541 tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg tttaataact
    21601 acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac tttggttacg
    21661 cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga gtccatcagc
    21721 tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata gaaaatcaga
    21781 ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg tgcacccgtt
    21841 gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt aatattaatc
    21901 aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta cgaagctctc
    21961 taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt agtaacgaag
    22021 gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa
    22081 gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga gtgggccagt
    22141 atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga acaaatgacc
    22201 atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc gacgtacgcc
    22261 ttcaaccagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt ctgccggaat
    22321 ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac ccacagccag
    22381 accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag atatatcacg
    22441 taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt taccaagact
    22501 gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag agcgttttgc
    22561 gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga tatccgaacc
    22621 aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat
    22681 cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa ggctgaagac
    22741 ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat cgtgcagatc
    22801 gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg tgttgccgag
    22861 gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta cggtgccgta
    22921 ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc
    22981 acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc cactacgtca
    23041 gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc gctcattaat
    23101 tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc
    23161 ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg ccgaaaccag
    23221 cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta
    23281 ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga taagatcgtt
    23341 ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac
    23401 ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc
    23461 gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc
    23521 cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta atttttccag
    23581 caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct
    23641 ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg
    23701 cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat cattaaattc
    23761 gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc
    23821 cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg
    23881 tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc
    23941 aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc cggggttgag
    24001 cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga
    24061 cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg attcgggcaa
    24121 ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa gagtcgggta
    24181 gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga atataatcga
    24241 tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt tctggcaatt
    24301 tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg
    24361 gcattcatat gcatcgacgt attgactgac aatctgccgg aagtccgcga agcacaggag
    24421 tggtttcgta gtgaaacgcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac
    24481 tttctttccc gccactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt
    24541 tatgcccgcg agcaagtgat gacgattttg ccggactcac cgccacgttt tatcaatctg
    24601 aacaattact tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag
    24661 aacgtgttaa acggcatggc aagccgccgc ccacgtctgg atgccctgcg tgactcctgg
    24721 tacgatttag acgctcatta tgacgccctc gaaacccgct tctggcagtt ttatccgcgg
    24781 atgatggcgc aggcgtcaca caaggcgtta taaaacgtta gttcatccat accgtagccg
    24841 aaatggcagg caaagccaga atcccgtcag tcagttgccc atgcccttct ttgcattgcc
    24901 attgcacggc attgagaaac ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt
    24961 tgattgccac cagtacacgt tgctgattca gcacgcggac aaataccact acgttatctt
    25021 ccgcatacag cacctgacag ccgccacgac gcagcgcctg acttttctta cgcagcacaa
    25081 tcattcgccg gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg
    25141 gtttacggca aaacggatcg tttttaccat ccagtcccac ttcatcaccg taataaatgc
    25201 acggtacacc aggccaggtg aacagccaga ccaccgccag cggcaggcgc gcaatatccc
    25261 gaccgagcag cgttttaaat cgcgcagtat cgtggctatc gagctgatta aacatacgta
    25321 attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg
    25381 cgtcaatttg ctgcggatca taagagatgt cggtattggc aagaaatccc cacaacggga
    25441 atgtgaagcc acgatagttc atggcagcat cttccacatc ggcctgtaac cattgccgtg
    25501 catcgccaaa atgttcgccg acaatatacg cttccggctg ggtttctttc gccgcttcgg
    25561 tgatcccggc aacgtgctgc atattattgc gcgccccacc cgcctccccc agcatatgca
    25621 ccacatccag ccgccagccg tccatattcc acggcgcttt cagccagtgg cggacaatac
    25681 tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc agcttcgtca
    25741 agctggcata gccaagccag tcgagcgccg tgccatcatc actaaacgag taccagtcgc
    25801 gccagggcga ttcagagttg tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc
    25861 aggcatggga atcgccactg tggttaaaca cgccgtccag caccagccgc attcccagct
    25921 gctgcgtatt gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat
    25981 ggcgataatc ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat
    26041 acagcgctgt cacgccaagc tttttcagat acggcagttt ttcgcttatc ccgtccagat
    26101 cgccgccata gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca
    26161 agatgatctc ttgtccggct gcatgatggt aatagacatg atcctgttca gcttcacgag
    26221 gaagactacg cgcaaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt
    26281 gtgggccgat atcaggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc
    26341 tgaagccctg cggtgtaaac caacgctggc gatcgtgcca cagcagtttg aaactgtaac
    26401 gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg acgcctggct
    26461 gcggctgact gcgctgctta tgcattggta ctgacatttc ttcgttatcg tgttctgtac
    26521 gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat
    26581 ctttgctttg tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc
    26641 ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga
    26701 aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt
    26761 cctcctgaac cggcttacag aacgcaagta ttattggttt tcagccagct gacgatcgtg
    26821 gcggcgttta aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag
    26881 cgagatacgc gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcag
    26941 cccgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc
    27001 tggcggcaaa cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccatac
    27061 cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt
    27121 cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc
    27181 gcgacgcgac gttttgctga aaattttcgg tgcgctgccc tgctctgcca taccgtggag
    27241 catacggcct acgccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac
    27301 aaagttaaga atgctggcgg caaaggtaat gcccatatgc tggaacgtca gtacgaacgg
    27361 gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc
    27421 gacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc
    27481 aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat
    27541 caccatttgc aacgacatta ccatgccaag ccagccgtta ctgaagaagc cgccgttgct
    27601 ccacaggtta tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc
    27661 gaaaccggcg acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaattc
    27721 cagctcaccg aagaccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct
    27781 cagtacccaa atccagtgcg gcaccgtcgg gaacaagaca cccatataga taccaaaagc
    27841 ggtcacatcg gcgatggcga caataaggat ttcaaaacag taggtccatc cggtaatgta
    27901 acctgccagc gggccaaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt
    27961 atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat
    28021 ataggccaac aacacgctcg gaccggccat tttgatggcg tctgccgaac cgtaaaacag
    28081 cccggtgcca attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg
    28141 ctttagctta ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg
    28201 ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac
    28261 ctgacgacct gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat
    28321 taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc
    28381 gctgaatgca gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg
    28441 cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag
    28501 tgcgatacac ggcggataaa tagcggtcag caccggtacg gagatctgga tcagctggct
    28561 taagccgagg ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata
    28621 agagagcggt acgtactggg caaagaattc tgcacaagca caggtcaggc caaccgccgt
    28681 gaccagacag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt
    28741 gtgctgaacg taagcatgca gaatagcagc gccgtttgca gactgatcga ccagcgacgc
    28801 gctgtctgaa cccagacaga acagcgccag gtacagcaaa gtcagaccaa cacccgccat
    28861 caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg
    28921 cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata
    28981 gccgttaacg aagccgttag aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat
    29041 agaacccgcc ggccagacaa tcgcgacaac agacaggatg accagcgcga taattttcag
    29101 cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat
    29161 aacgatagcg aaatagacca ggctgtagat aaacagcggc agcgcggaat cacccgtcag
    29221 cggcgcaatc cccacttcaa aggaaacggt agctgtacgc ggcgtagcga aaagcggccc
    29281 caccgccagg taacaaaccg ttgccagcag tacgccagcg actttaccga ttggcgtgct
    29341 gaggctgtca acaccgccgc caacttttgc cagcgccact accgttaata ccggcaggcc
    29401 aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc
    29461 gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc
    29521 cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg
    29581 tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa
    29641 acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc
    29701 gcgttaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta
    29761 aagcaaggag ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata
    29821 aaacactatt tccgtgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt
    29881 gtaaaatcta acgcccgtat tttgactgca aagaaaataa gccagcatcg gctggcttat
    29941 ggcaataaaa gatgacaaag gcggattaat cgctgttttt ggcaattaaa cgttccggga
    30001 taacaaaact gaaacgtgtt ccttttccta ctgtactctc aatattcagg cgactttcgt
    30061 gatgattcac cgcatgtttc acgatcgcta accctaatcc gctaccgccg gtttgccggg
    30121 aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa
    30181 tgcccggtcc gttatcttca acgctaaatt cggcaccgtg cggcactcgc tgccagcgta
    30241 cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg
    30301 cactgcgtag ctgatcttcg ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa
    30361 atgtctgttt tttctgactc agagtctgag cctcgcgctc aacaacgcgc agcatcatcg
    30421 gcacatcaac cttttcattg agcaattgcg tcggcgcggc ttcaattttc gacagcgtta
    30481 gcaactgctt caccagccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt
    30541 tttcgcgtac cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca
    30601 cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt
    30661 ccagttgatg catttgcgtg acatcacgcg ccaccatcag caactgtttg tgggtataag
    30721 gcatgacgcg aatttccaga tgccgcccgg tgttgagcac cagattgagc gggcgagaaa
    30781 aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagtaggtta aggatgttct
    30841 gcccgttatc ttccggccag cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga
    30901 aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac
    30961 gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct
    31021 ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc
    31081 gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc
    31141 ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag
    31201 ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca
    31261 cgtaagatac tccagttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt
    31321 aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc
    31381 cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca
    31441 catacacgtt agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag
    31501 gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agcggctctt
    31561 cgcccgccat cactcggtga gatgtcgggt tgagacttaa tccctgcatc ttaatcacct
    31621 cttccaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct
    31681 tcggcgaaaa tggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat
    31741 cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc
    31801 gcttgaggtg tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa
    31861 ttaaatccgg ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga
    31921 ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat
    31981 cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat
    32041 cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac
    32101 atatcttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg
    32161 tataatcgcg accatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga
    32221 ctggcatctc ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct
    32281 tgactggctg ctggagacag cacaaaccca tcaggtggat gcgattattg ttgctggtga
    32341 tgttttcgat accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt
    32401 caatttacag caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc
    32461 cacgctgaat gaatcgcgcg atatcatggc gttcctcaat acaaccgtgg tcgccagcgc
    32521 cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc
    32581 cattccgttt ttacgcccgc gtgacattat ttccagccag gcggggctta acggtattga
    32641 aaaacagcag catttactgg cagcgattac cgattattac caacaacact atgccgatgc
    32701 ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt
    32761 gggttccagt aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc
    32821 ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat
    32881 tattggcggc atggaacatg ttcgctattg cggttccccc attccactga gttttgatga
    32941 atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt
    33001 ggaaaacctg aacgtaccgg taactcaacc catggcggtg ctgaaaggcg atctggcgtc
    33061 gattaccgca cagctggaac agtggcgcga tgtatcgcag gagccacctg tctggctgga
    33121 tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac
    33181 cgaatcattg cctgtcgaag tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt
    33241 gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg
    33301 ccgtctggca ctggaagacc tggatgaatc gcagcagcaa cgtctgcagc atcttttcac
    33361 cacgacgttg cgtaccctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg
    33421 aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc
    33481 agcaacgggc tgtttgccat taccggccca accggtgcgg ggaaaaccac cctgctggac
    33541 gccatttgtc tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa
    33601 aatgatctca tgacccgcga taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa
    33661 ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc ggacggtaat
    33721 ttgcaggtgc ctcgcgtaga gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa
    33781 gtgaaagata agctggaact gacagcgacg ttaaccgggc tggattacgg gcgcttcacc
    33841 cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa
    33901 cgcgcggaat tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg
    33961 gtttttgagc agcacaaatc ggcccgcact gagctggaga agctgcaagc gcaggccagc
    34021 ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt
    34081 actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg
    34141 ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag
    34201 gcgttagccg aagaagaaaa agcgcaacct caactggcgg cgcttagtct ggcacaaccg
    34261 gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat
    34321 attcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgcgcg
    34381 agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg
    34441 aatacctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg
    34501 cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag
    34561 ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc
    34621 gatgaagttg ctaccgccct ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg
    34681 gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt aatggtcact
    34741 atccagaatg tcactctgga acagacgcaa cgtaatgtcg cacttaacga aatgcgccag
    34801 cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg
    34861 cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg cgggtcagcc ttgtccactt
    34921 tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat
    34981 cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg
    35041 ctgcgcgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa
    35101 agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc
    35161 aatatcacct tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac
    35221 gaacgccagc tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat
    35281 aatcagcaaa ttatccagta tcaacagcaa attgaacaac gtcagcagca gctcttaacg
    35341 gcattggcgg gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg
    35401 acacgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa
    35461 aaccgtattc agcagctgac accgattctg gaaacgttgc cgcaaagtga tgatctcccg
    35521 cacagcgaag aaactgtggc gctggataac tggcggcagg ttcatgaaca atgtctcgca
    35581 ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa
    35641 aaagcccaag cgcagtttga caccgcgcta caggccagcg tctttgacga tcagcaggcg
    35701 ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat
    35761 ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctggca
    35821 cagcatcaac aacaccgacc tgacgggttg gctctcactg tgacggtgga gcagattcag
    35881 caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt
    35941 cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa
    36001 attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc
    36061 aaagagggcg ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat
    36121 ctcgctaatc agcaacttac ccggctgcac gtgcgctatc tgttacagcg caaagccagc
    36181 gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag atgcggtacg cgatacccgt
    36241 accctttccg gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc gctttcggat
    36301 ctggtcagcc ataaaacacg tattgactcg ctgttccttg atgaaggttt tggcacgctg
    36361 gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa
    36421 accatcggtg tgattagcca cgtagaagcg atgaaagagc gtattccggt gcagatcaaa
    36481 gtgaaaaaga tcaacggcct gggctacagc aaactggaaa gtacgtttgc agtgaaataa
    36541 ctattcagca ggataatgaa tacagagggg tgaattatct cttggccttg ctggtcgtta
    36601 tcctgcaagc tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg
    36661 gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatggccg
    36721 aatttggcat tatgagcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg
    36781 ctgccgggca tatgatctcg tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg
    36841 cactcttttc cagccgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg
    36901 tcattggcaa cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc
    36961 tggtatccgg ctttccgcat ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa
    37021 ttatcaaacc cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg
    37081 ccaatttgct gggcattccg ctgggaacgt atttaagtca ggaatgctgg cgttacacct
    37141 ttttattgat cgctgttttt aatattgcgg tgatggcatc ggtctatttt tgggtgccag
    37201 atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg
    37261 ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc aggtgtgttt gcctggttca
    37321 gctacgtaaa gccatacatg atgtttattt ccggtttttc ggaaacggcg atgaccttta
    37381 ttatgatgtt agttgggcta gggatggtgc tgggaaatat gctaagtggc aggatttcag
    37441 gacgttattc accactgcgc attgcagcag tgactgactt tatcattgta ctggcactgc
    37501 tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct tatttttgct tttatttgtt
    37561 gcgcgggatt atttgccctt tcagcacctc tgcaaatatt gttactgcaa aatgccaaag
    37621 gaggagagtt attaggtgcc gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg
    37681 gcgcatattg cggaggtatg atgctgacgc tggggctggc atataattac gtggcgctgc
    37741 ctgccgccct gctttcgttt gctgcgatgt catcgttgct gctgtatggt cgctataagc
    37801 gccagcaagc ggcggatagt ccggtgctgg cgaaaccact ggggtagatt actgtcacgg
    37861 tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat ccgccctacg cggttctggc
    37921 acattttgta ggcctgataa gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc
    37981 ctacgttttt tactcttgtg gccataacca cgcagcgccg cgtacgccgc tggaatcacc
    38041 gtgcttcgcc ttactcaccg gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa
    38101 ctgcccaacc gtttgatata aacggtctac attgctcatc ccgcccccca ggacaatcac
    38161 atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca gctcgtagcg
    38221 acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggcggataa tttcactgcc
    38281 tttcagcgca tgtccgctca aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa
    38341 ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat aacgcagttc
    38401 gtcttcgtcc atccacggta gcggattgtg tccccactca cctgccgtgc cattgccgcc
    38461 gatatgcgtc cgcccattga atgccacgcc cgcgccgcat cccgtgccga taatcacggc
    38521 aaataccgtc tgcgctcccg ctgccgcgcc atctactgct tctgaaaccg ccagacagtt
    38581 ggcatcattt gccagccgca cttcccgctg caacctcgcg cttaagtctt tatcgaatgg
    38641 ctgaccgttg agccaggttg aattgacatt cttcaccaca ccggtgtagg gcgaaatcga
    38701 acctggaatg cccataccta ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac
    38761 caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg
    38821 gcggtacaac tgctcccctg catcgcccag tgcaatcact tcagttttgg tgccgcctaa
    38881 atcgatacct atacgcactg tactctcctt atttttttca atatcaatag cgtagagacg
    38941 gacaaccgga ttggcaatgc aaggccgccg acaattcgtt atcatgcccg ctaaatttaa
    39001 cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt accgtcttag
    39061 ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga tggcatttac
    39121 cccatgcggc agccaggaca tggcgaagat gggctgggtt cctccgatgg gatcgcacag
    39181 cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa
    39241 aatcctcccg tctccggtga ttaaacaggc gctggaagcg aaaatcgcca aactggaagc
    39301 ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg aagtgctgca
    39361 ctctctgctg ccgcgtgctt tcagccgttt tagccagaca atgatgtggc tcgacacggt
    39421 taacggtttg attatggtgg actgcgccag tgccaaaaaa gcggaagata cgctggcatt
    39481 actgcgtaaa agcctggggt cgttaccggt tgtaccattg agcatggaaa acccgattga
    39541 actgacgctg accgaatggg ttcgctccgg tagtgcggca cagggcttcc agctgcttga
    39601 tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga agaaacaaga
    39661 tctgaccagc gaagagatca ccaatcacat tgaagccgga aaagtggtga ctaaactggc
    39721 gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct
    39781 taagttctgc gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca
    39841 gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct
    39901 gattgaagga ttaggtggcg aagcacaacg ttaattgctg attttccttt aatgccggat
    39961 gcgacgcctg ccgcgtctta tccggcgtac gaagccacac caggcatata attattcgct
    40021 acggcgagca ataattttta gcgcagcaat attatgcgtt ttacgccgta actgatctaa
    40081 tcgcctcatc cctattcgcc tgtcagcagc acgcagaata ttcactaacc cccatttatc
    40141 gctggtaaat tgttttgtta acagctccgc ttttggggta tcaatatatt cacgcaataa
    40201 atcagaaatt gaacaaatat ccgtcaggta aggataccag caagtttcac cgtgaaaatt
    40261 acgcactcca gcgttacctg caataaagtc tttcgggtaa tcccagatca cgtttttatc
    40321 caacgtgatc caataacgcg gtaaatcggt acttccacca ttttggctgc gcatgggata
    40381 acgggtacag tgaatttgca ggttgatcgt tggcgtcagt aagtcataga tctcccgctg
    40441 taacttgctc catggacgtt gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg
    40501 cgaattttcc gcgttgcccc ggttttcact aaatcacgta actgcgaaag atcgggttgt
    40561 tcgacaaata agagtaatga tggatcctct tttaaccacg ccgttttcac atcggcagcc
    40621 agctgtggat tattaatagc cagcgaccga tcctgaggct cagtcaatgt cgttaacaac
    40681 gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc
    40741 agcaatgcac gccaccattc atcatctgat ttatctttta tcgtagccag ttccgcaaaa
    40801 aatacctgga gatcttcact gatatttata tcaaccttga tgaattatga atatatcgga
    40861 aattgaaaaa atcgcagcaa aggaaatatt attatgggtt aaagagagga tatttcagaa
    40921 aaatgccctc ccgttcaagg ggaaggcgag gaattacaga tagcggcaca gataagaggt
    40981 aggttcggca acttgaagat gaaactcact gtgaccggga acattaaaca ccgaaccggc
    41041 ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac
    41101 cgtcatctct tccggctcag cggtgctgaa ggtgtattcg ccttcaacca taacacccac
    41161 gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat ttcactttgc cggaaaagta
    41221 ctcattactt tgaagcataa actggccctg tctggtagtt gaacatctac ttcactatag
    41281 gggccagagg cgctggctgt cacgcaaaat tacaccagta attccgcagc cagccgtgca
    41341 atcaatacgt tagagagcaa gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga
    41401 cgttggtgaa atcctaaaca atccagcatg atgacatcag ccccttttgc cagtaattct
    41461 ttcccagcat caatgatttt ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc
    41521 ggcgatttct gcaaaatttg ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga
    41581 acgataaccc ccacctgatg atcttcaaca atagaggaaa ccagtggagg caatattcgc
    41641 gacggctcaa gaaagatcgt attacgcgca gtcatactac taatgtttgc tgtactcatt
    41701 aataaaatga cgtcataacc ctgattatcg agcacttcaa ccacaccttg cagatctcgc
    41761 tccactttac gacgcgaaac atgggccagc tggttgtcat ttaataatgt gagaatggtg
    41821 tcttcgcctg cttctggcgc gtactccgcc atcacttctt cacgacttaa cttccccagc
    41881 aggctatgat gggaaatatt atcttcgtca atgtattccg tcaggagcgg caaaacctcc
    41941 tgcataggta caatgccgat ggtcaggatc gccaacgacg cactcattgt tcatccacct
    42001 tacttttctt tcgtcgttaa ttaccgggca agtgtgaagc accactgctg acacttactt
    42061 gtgctacaaa tgacaaaaag cgtagcagca gacgtgcacg gcataatgta aagattccaa
    42121 atgattccag taatggaatg ttattgttta atattctaat tattagaaaa acataaatta
    42181 tgaaaaatgt gacgcagatc ccttgtagtg gcactcagaa tcccttccga gcagtctgat
    42241 gcggtcactc tttgtcaggg tcctcatagc gttttttcgc atccatcgct tcctgagccg
    42301 tcggatgttc actgatcaac gagtctggtt taggatgatc ggctctgagt tggtaccagg
    42361 ttaccgtctg tcctggcttt cctttttcaa tcgtcactat atatgcttca cgaggataag
    42421 gcggtttcgt tggcatcgtt cttccttatt tcacgggatg aacgttaagt ataggcgctc
    42481 taaaatcaac aattgatcgt ctgtgccagg gcgctacgaa tttcagaaat cacctggctg
    42541 ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc gatatagcgc atcgcgttct
    42601 tccagcactt cctgaacttc ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct
    42661 tcttccggtg cagcttgcag tcggttaacc aggactgata ctggcgcaca caaataaacc
    42721 acgatcccgt tattttgcat gaagtgacga ttaaattccg tcagaataat gccgccgcct
    42781 gtagcgataa cggtggatgc cgcagttacc gcttccagcg ccgccgtttc tctggcgcga
    42841 aatcccgccc actcttccct ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc
    42901 aaccactgat cggtatcgac aaaccgacgg ttaagcgaat cggcaagggc cattccgacc
    42961 gttgttttac cacagccccg aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt
    43021 ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa
    43081 actagtacaa tttcgattgt aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc
    43141 ccttactata ccaataaata ttcaagaatg aagtgtaaat aataaattac atttagccac
    43201 gactacgttg cacttccagc caccacttct ccagctccgc ggcaaaggcc tggcggtcac
    43261 gttgtgaaag gctatctggt ccgccggtct ggatcccact ggcacgtaag gtatccataa
    43321 aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat
    43381 taagcgccgc tgcgcctttc tcgatggctt cagcagccaa aggtatatct gcggtgatca
    43441 ccaaatcgcc cgcttcacac tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg
    43501 cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg taaactctgg tttgctacca
    43561 gtaccagcgg catctgcata cgttccgccg cgcgatacaa aatctcttta attacattgg
    43621 gacacgcgtc ggcatccacc caaattgtca taaagtcatc ctttgttagg taatcctcta
    43681 ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg cttctgccag cgattatcaa
    43741 aacaatgaat ttcacggcag gagtgaggca atggaaaaga aaatcggttt tattggctgc
    43801 ggcaatatgg gaaaagccat tctcggcggt ctgattgcca gcggtcaggt gcttccaggg
    43861 caaatctggg tatacacccc ctccccggat aaagtcgccg ccctgcatga ccagttcggc
    43921 atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc
    43981 gttaaacctg gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac
    44041 tctctggtcg tttctattgc tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc
    44101 catgaccgga aaattatccg cgccatgccg aacactcccg cgctggttaa tgccgggatg
    44161 acctccgtaa cgccaaacgc gctggtaacc ccagaagata ccgctgatgt gctgaatatt
    44221 ttccgctgct ttggcgaagc ggaagtaatt gctgagccga tgatccaccc ggtggtcggt
    44281 gtgagcggtt cttcgccagc ctacgtattt atgtttatcg aagcgatggc cgacgccgcc
    44341 gtgctgggcg ggatgccacg cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt
    44401 tccgcaaaaa tggtgctgga aacgggagaa catccggggg cactgaaaga tatggtctgc
    44461 tcaccgggag gcaccaccat tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc
    44521 gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag caaatcctga
    44581 tgactttcgc cggacgtcag gccgccactt cggtgcggtt acgtccggct ttctttgctt
    44641 tgtaaagcgc caaatctgcc gatttcaacc actcacgata gtgactcatt tgtgggttca
    44701 gcggcgcaac ccccacacta atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg
    44761 tgtttagccc ttcacgcacc cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg
    44821 acatgattac tgcaaactca tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg
    44881 taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca tgaccccagg
    44941 tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca tcgcgattat
    45001 gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt cggttataca
    45061 cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt tccgccagtt
    45121 tggttgccgt ctggtagctg acccagccaa acatcagagg ataaacgaca ataatgggaa
    45181 gggagagcca ccattccagc ggagcactat tgaacgacac ggtaatgccc gtaagctcga
    45241 gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga cgggggccgc
    45301 ctgcccccat caaattcaga cacagaatca tcaacatagc ggtggaaggc agcacgttta
    45361 cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag ttgtaaattt
    45421 cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc ggccagacga
    45481 acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa accagcgttg
    45541 aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg acgcgacggg
    45601 cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt tcctccccat
    45661 gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac attcagaaac
    45721 aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc gggtgagcca
    45781 ctttcgtagc tcacccgtta gcactatctg aaccgccccg ggaatcctgg agactaaact
    45841 tcctgagaaa gaggtaaaca ggatgactaa aaatactcgt ttttcccccg a
//
LOCUS       DAJTOW010000024        40391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTOW010000024 DAJTOW010000000
VERSION     DAJTOW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.024"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..143)
                     /locus_tag="OO244_001404"
     CDS             complement(<1..143)
                     /locus_tag="OO244_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663287.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEA"
     gene            complement(408..668)
                     /locus_tag="OO244_001405"
     CDS             complement(408..668)
                     /locus_tag="OO244_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5663288.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            944..1870
                     /gene="rlmF"
                     /locus_tag="OO244_001406"
     CDS             944..1870
                     /gene="rlmF"
                     /locus_tag="OO244_001406"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5663289.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(1867..4091)
                     /gene="ybiO"
                     /locus_tag="OO244_001407"
                     /pseudo
     CDS             complement(1867..4091)
                     /gene="ybiO"
                     /locus_tag="OO244_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(4352..5074)
                     /gene="glnQ"
                     /locus_tag="OO244_001408"
     CDS             complement(4352..5074)
                     /gene="glnQ"
                     /locus_tag="OO244_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5663290.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(5071..5730)
                     /gene="glnP"
                     /locus_tag="OO244_001409"
     CDS             complement(5071..5730)
                     /gene="glnP"
                     /locus_tag="OO244_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5663291.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(5869..6615)
                     /gene="glnH"
                     /locus_tag="OO244_001410"
     CDS             complement(5869..6615)
                     /gene="glnH"
                     /locus_tag="OO244_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5663292.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            complement(7019..7522)
                     /gene="dps"
                     /locus_tag="OO244_001411"
                     /gene_synonym="pexB"
     CDS             complement(7019..7522)
                     /gene="dps"
                     /locus_tag="OO244_001411"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5663293.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(7821..8708)
                     /gene="rhtA"
                     /locus_tag="OO244_001412"
     CDS             complement(7821..8708)
                     /gene="rhtA"
                     /locus_tag="OO244_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5663294.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8943..9008
                     /locus_tag="OO244_001413"
     CDS             8943..9008
                     /locus_tag="OO244_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663295.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            9061..9576
                     /gene="ompX"
                     /locus_tag="OO244_001414"
     CDS             9061..9576
                     /gene="ompX"
                     /locus_tag="OO244_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5663296.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(9624..11207)
                     /gene="opgE"
                     /locus_tag="OO244_001415"
     CDS             complement(9624..11207)
                     /gene="opgE"
                     /locus_tag="OO244_001415"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5663297.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(11479..11607)
                     /gene="mntS"
                     /locus_tag="OO244_001416"
     CDS             complement(11479..11607)
                     /gene="mntS"
                     /locus_tag="OO244_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5663298.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            11793..12260
                     /gene="mntR"
                     /locus_tag="OO244_001417"
     CDS             11793..12260
                     /gene="mntR"
                     /locus_tag="OO244_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5663299.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            12257..13375
                     /locus_tag="OO244_001418"
     CDS             12257..13375
                     /locus_tag="OO244_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5663300.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(13433..14353)
                     /gene="ldtB"
                     /locus_tag="OO244_001419"
     CDS             complement(13433..14353)
                     /gene="ldtB"
                     /locus_tag="OO244_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5663301.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            14572..16164
                     /locus_tag="OO244_001420"
     CDS             14572..16164
                     /locus_tag="OO244_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5663302.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(16405..17670)
                     /locus_tag="OO244_001421"
     CDS             complement(16405..17670)
                     /locus_tag="OO244_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5663303.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(17822..18637)
                     /gene="ybiV"
                     /locus_tag="OO244_001422"
     CDS             complement(17822..18637)
                     /gene="ybiV"
                     /locus_tag="OO244_001422"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5663304.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(18783..21215)
                     /locus_tag="OO244_001423"
     CDS             complement(18783..21215)
                     /locus_tag="OO244_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5663305.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(21221..22120)
                     /locus_tag="OO244_001424"
     CDS             complement(21221..22120)
                     /locus_tag="OO244_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5663306.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            22251..22913
                     /gene="fsa"
                     /locus_tag="OO244_001425"
     CDS             22251..22913
                     /gene="fsa"
                     /locus_tag="OO244_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5663307.1"
                     /translation="MELYLDASDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            complement(23092..23841)
                     /gene="moeB"
                     /locus_tag="OO244_001426"
     CDS             complement(23092..23841)
                     /gene="moeB"
                     /locus_tag="OO244_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5663308.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(23841..25076)
                     /gene="moeA"
                     /locus_tag="OO244_001427"
     CDS             complement(23841..25076)
                     /gene="moeA"
                     /locus_tag="OO244_001427"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5663309.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            25280..26245
                     /gene="iaaA"
                     /locus_tag="OO244_001428"
     CDS             25280..26245
                     /gene="iaaA"
                     /locus_tag="OO244_001428"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5663310.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVIMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYGIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            26232..28103
                     /gene="gsiA"
                     /locus_tag="OO244_001429"
     CDS             26232..28103
                     /gene="gsiA"
                     /locus_tag="OO244_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5663311.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            28123..29661
                     /gene="gsiB"
                     /locus_tag="OO244_001430"
     CDS             28123..29661
                     /gene="gsiB"
                     /locus_tag="OO244_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5663312.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            29679..30599
                     /gene="gsiC"
                     /locus_tag="OO244_001431"
     CDS             29679..30599
                     /gene="gsiC"
                     /locus_tag="OO244_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5663313.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYRQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            30602..31513
                     /gene="gsiD"
                     /locus_tag="OO244_001432"
     CDS             30602..31513
                     /gene="gsiD"
                     /locus_tag="OO244_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5663314.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            31691..34039
                     /locus_tag="OO244_001433"
                     /pseudo
     CDS             31691..34039
                     /locus_tag="OO244_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            34047..35375
                     /locus_tag="OO244_001434"
     CDS             34047..35375
                     /locus_tag="OO244_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5663315.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(35422..36747)
                     /gene="rimO"
                     /locus_tag="OO244_001435"
     CDS             complement(35422..36747)
                     /gene="rimO"
                     /locus_tag="OO244_001435"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5663316.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            36960..37343
                     /gene="bssR"
                     /locus_tag="OO244_001436"
     CDS             36960..37343
                     /gene="bssR"
                     /locus_tag="OO244_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5663317.1"
                     /translation="MFVDRQRIELLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            37454..38568
                     /gene="yliI"
                     /locus_tag="OO244_001437"
                     /pseudo
     CDS             37454..38568
                     /gene="yliI"
                     /locus_tag="OO244_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(38565..39191)
                     /gene="gstB"
                     /locus_tag="OO244_001438"
     CDS             complement(38565..39191)
                     /gene="gstB"
                     /locus_tag="OO244_001438"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5663318.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            39437..>40391
                     /locus_tag="OO244_001439"
     CDS             39437..>40391
                     /locus_tag="OO244_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5663319.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAG"
BASE COUNT         9837 a         9832 c        10672 g        10050 t
ORIGIN      
        1 gcttcgacaa ccagcgtgtc atccgcgtta gcttgccctt cggcggcgag tgcctgtgcc
       61 acaggcgtaa taccaataca aagaccggca aagaacgtga gcgaatgaaa ttgtctggca
      121 gggaaattgc gattgttttc cattttgcag gtgacttttt cttatatgtt gaaattttca
      181 ctgccacgag aaagcatctc tgcggatgcg atgccactgt taatcccgtg atagtgttcg
      241 tgcaagtgat aataattttg ataagcatta tcacttgatg cgattttcta tcacccattg
      301 cgcgaaaaag aaatacattt atttacattg cgttcatatt tgatgttttt tgattacaaa
      361 acaggcgaaa actgcatcca gaggcgaatg cagttacgtt ggtggggtta tttgtagatg
      421 gttgcagtac ctaacatctg gtcatttccg cctgcggaat taatgacata acctttcgcg
      481 ccttgttcgc gcgctttttc tgccagttta tcctccagat cgcttaggtt tgatgcgcca
      541 gttgccgaaa cggtaccggc gggccgtagc tgaccgctgt ccaggttact catcggttgc
      601 gcggcataag ccgtgagaga gattccgctc aggacggtgg caatcagtag tgtgaggcac
      661 tttttcataa tgacatcctc ataaatacaa cgggtagtgc ctgataagtg taggtcgcct
      721 gcgcgatgca aatgacgcaa aaaattgaca gagaaatgct tattttttga acgatatatt
      781 tttacaaaat aagacaaatc aatgacatgc ataatgctga tttttatgat gaattgccat
      841 aaaccatagc catgttaagg ttttggggaa tgataaacgc agccagatat ttaccctgtc
      901 gcgttatgcg ggtatcttac gccgctgctt aaaggagaat gctatgtccg cccagaaacc
      961 ggggttgcat ccgcgcaacc gtcatcacag ccgctacgat ctcgccacgc tttgtcaggt
     1021 caatcctgaa ctcaggcaat tcctcacgct tacacccgca ggggagcaaa gcgtagactt
     1081 tgccaatccg ctggcggtga aggcgctcaa taaggcgttg ctggcccatt tttacgccgt
     1141 agcgaactgg gatatccccg acggttttct ctgcccacca gtaccgggcc gggcggatta
     1201 tattcatcac cttgccgatt tactggctga agcaagcgga acaattccag cgaatgccag
     1261 cattctggat atcggcgttg gtgcgaactg tatttatccg ctgattggcg tacatgaata
     1321 tggctggcgt tttaccggta gtgaaaccag cagccaggcg ttaagcagtg cgcaggcgat
     1381 tatcagtgct aatccggggc ttaaccgcgc cattcgtctg cgtcggcaaa aagagagtgg
     1441 ggcgattttt aacggcatca tccacaaaaa cgagcaatac gacgcgacct tgtgtaaccc
     1501 gccattccac gattccgccg ctgcggcgcg ggcaggtagt gagcgtaaac gccgtaacct
     1561 ggggctgaac aaagacgatg cactgaactt tggtggtcag caacaggagt tgtggtgtga
     1621 aggcggtgaa gtcaacttta tcaaaaagat gattgaagag agcaaaggct tcgcgaagca
     1681 ggtgatgtgg tttacctcgc tggtatctcg tggtgaaaac ttaccgccgt tgtatcgtgc
     1741 tctgaccgac gtgggcgcgg tgaaggtggt aaaaaaagag atggcccagg ggcaaaagca
     1801 gagtcgcttt attgcctgga cctttatgaa cgacgagcag cgccgccgtt ttgttaatcg
     1861 ccaacgttaa agcgttggct cccccggcgg cagcggttca gccggggtcg cgcccggagc
     1921 aggcagcacc tgataagtct gcactggcgc gcgaacgccc gccaggtcga aatgtttttt
     1981 cacctggctg tcgagggcaa agcgtaccgt ccactgtttg agtggcagcg tggtgaacga
     2041 aacacgcagt gtaaacgcgg tattgcttaa gccgacaatc ccggcaaagt tcggttcacc
     2101 aataatcagc ccgcgaattt cttcgttttc catcaattcc gttaccgcat ctttcagtgc
     2161 ctgattggct ttatcagcat cttcatggcg atcaacatcg tagtttgcca ctaccgaacc
     2221 aataccgcgg acgaagttag caaaggtggt tatcgaagac cacggaatga tgtgatacgc
     2281 cccggtatcc tggcgcacgc ccacggagcg aatcgacatc cgttccactg tgccggtcaa
     2341 cggcccgata gtcaccaaat ctccagtgtt catgccgttt tcaaactgaa taaatacccc
     2401 ggtgataata tcttttacca gcgtttgcga accaaacgag atagccagac ctaatgcccc
     2461 ggcacctgcc agcaatggcg cgatattgac gccgatttcc gacaacacaa tcatgatggt
     2521 gatggtactg ataatcaccg ccagcgcgtt acggaacagc gtcagcaggg tacgcgtacg
     2581 ggcgctgggt agcgggcggc catgaatatc cgaagccagc cggttttcga tcaaactggc
     2641 gagcaccgtc cagccaaccg ccgagaagaa aagaatgagt gcgatgcgga tcaggatatc
     2701 tacggttttc tgccccgcgc cgttttgcag ccagttccag aaatcgaaca atccccatgc
     2761 gctcaacagc aacattactg ccacgcagac ggtcagaata cgcgccgttt tcagcgccgc
     2821 cgaaagccag ccattcagcc gtttttgtag ctccggatag ttacgctgag tatgtggcga
     2881 gagggtgatg gttttcgcca gccagcggga aaacataccg gaaacaaacg ctgcgatacc
     2941 aataatcgcc aggctgcgca ccgttgcacc catcataaat ttcagactgt tgcccggatc
     3001 gaacaacgaa aagaaaaaca gcatgataaa ataggcgctt gccagccagt gccacaccag
     3061 cgcaaaggcg cggataaaca ggctgaaaaa agccagcgaa tgctccgcaa agttgagcat
     3121 atgctgggta atctcttttt tatttcggaa gatcaggtac aacgcccaga cggtcatgca
     3181 cagcataatg atgacgttcg ccagcgcacc tatctgtaca ttcacctgat tagagataat
     3241 cggcacggcc acaatcaggc catagccaat caggctgctt aaccagctca ggcgacgacc
     3301 ccagtaacgg gcgctctcgt cctgaatcgt gaacgggcgc agctccgcca cgtttgggca
     3361 aaaaatcagg cgtagtacgg ctttgaaaaa ttcaatcagg gcaaaggcgt tgagaaacaa
     3421 actttgttgg aaagcgatgg tgcgactgcc tgcattcagg ttgtcgctta atacctggcc
     3481 gacaaataat gtcagggcca gtaacagcag gtcgataata aacgccccaa taatcatcgc
     3541 cggaagctgc aaccagttgc tgcgctcacg atttttttgc ctcgcccact gacccatttt
     3601 gcgatatagc ggcagtgcgc acaggcgaat cagccagtaa aaaccaaaca ctaataccgc
     3661 taacattgaa aaatggctca gcgcattgct gaaggtttgt ggattaaatg gcttatgcgg
     3721 ggagccggtg atattgcgat aaagttgccc gaagcgggcg gaaagggctt caccataatg
     3781 gcggctgact tcggtgacct tttgcagcac ggtttgctct tcaaccagcg tcggcggcac
     3841 gatcttcggg accggttccg cagggggcgt cgcggcgaca gtacgcaact ggtcgatcaa
     3901 ctctttacgc gaggtgtcgt tatccagcac atccgccagt gcgccatagg ccgctttttt
     3961 tgttcgatat ccggttccgg ggccggttca gtcgttgagt ccgttgtcgt tgtggttgta
     4021 acgccgggta tggataccgc gtgggcaggt gcgcccagca ggcagaagag gatgaacagg
     4081 atccaccgca tgactcctcc agtgagaaaa cagagcaaaa agataagtat agatgctggg
     4141 ggaggggcga ttttaaatga gaggaatctg gtgtgcctcc ctttcgggtg aaagggagga
     4201 agggatatta agcaggttgc tgacaacgtg cgggcttttt tgccggatgc ggcgtgaacg
     4261 ccttatccgt cctacaagtt catgcaattt taataagttg ccagatatcc tgtaggcctg
     4321 ataagcgtag cgcatcaggc aatgtgtctt attaagagac gtgctgcaaa aattcctgca
     4381 agcgctggct cggcgggttc ttgatcaaca cctgcggatt gccatcttcc gcaatccggc
     4441 ctttgtcgat aaagatcagc cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca
     4501 cgatcaccat cgtcatccct tcttcagcca gatcctgcat aaccttcagc acttcatggc
     4561 gcagttccgg gtcaagagcg gaagtcggtt catcaaacag catcattttc ggcttcaccg
     4621 ccagcgcgcg ggcaatcgcc acacgctgct gttgaccacc agaaagttcg gaagggtagt
     4681 gatgtgcacg ttctgccaga ccgactttcg ccagcagctc acgcgccagt ttttccgcct
     4741 cttctttgtt cgcgccacgc acgcgtagcg gaccaaacat gacgtttccc agcgccgtca
     4801 gatgcgggaa gaggtaaaac tgctggaaca ccatacctgc ttcctggcga atcaggcgct
     4861 cgtcaacttt cggatcgttg accttcaggc catcgacaat cagatcgccg gaggtgattt
     4921 cttccagttt gttgatgcag cgcagcaggg tcgatttacc ggaaccggac ggcccgataa
     4981 tcaccacgac ttcgccctgg gcaatgttca aatcgatatt gtgcagcacc tgggttgggc
     5041 caaagtgctt ggagacgttt ttaaattcaa tcacaggatt ttcatccttc tttccagacg
     5101 acgcagaata aagctcagca ccagggtaat aatcagatag aacaccgcca cggcgctcca
     5161 gatctcaagg gcgcggaagt taccggcaat aatttcttgc ccctgacggg tcagttccgc
     5221 cacgccgata acaataaaca gcgaggtgtc tttaatgctg atgatccact ggttacccag
     5281 cggcggcagc atacgacgca gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg
     5341 tgaaagaccg agcgccagtc ctgcttcacg aaaacctttg tggatagaca gcaccgcacc
     5401 acgcgtgatt tccgcaatgt acgcgccgga gttgatcatg atggtgacca ccgccgcagt
     5461 aaatgggtcg atgcgtaagt cgttaaacgc catcggcagg gcgaaataga taaacatcac
     5521 ctggacgacg ataggtgtgc cgcggatcac ttcaataaag accagcgcga cgtggttggc
     5581 tatccaacca ccgaaggtgc gtgcaaaacc tgccagcaat ccgattacca gaccgccagc
     5641 cagaccgagg accgaaatcc acagggtcat tttggcacct tcaatcagaa gcggaatggc
     5701 aggccagatg gcactccagt caaactgcat atgttgttcc tgttaccgtg gtgtaaataa
     5761 tcaaattcag ggtaattaat ggccccggag cgggtttgcg cccctcaccc taatcctctc
     5821 cccagggggg agagggaact gccagtgcgc tttacaggtg tagcgttatt atttcggttc
     5881 agtactgaac cattttttgt agatttcgtt gtaagttccg ttctcgcgca gggttttcaa
     5941 cgcgccgttg actttgtcac gcagctcgtc gctacctttc gggaacgcaa taccgtattg
     6001 ctgcgcttcc agagagtcac ctaccgcttt gaactgaccg ttaccggcgg ttttgatgaa
     6061 gtacagaatg tttggcgtat cgtgcagaac ggcgtctgcg cggttggtgc ccagttccat
     6121 ataggcgtta tcgatgttcg ggaactgacg cagatcttta gttttgatgt ttgctttcgc
     6181 gtaatcaacg gagccagtac cgctcttcac agcaaccact ttcccgtcga gatctttcac
     6241 gcttttcaca tcgttattgt tagctttcac catcactaac aggccgcttt tgtagtagcc
     6301 gtcagagaaa tcgatcgctt ttttacgctc gtcggtgatg gtaatgcccg ccagcgccag
     6361 atcgacgttt ttggtttgca gtgccggaat gatcccactg aaatccatcg gcttcagttc
     6421 gtaatccagc ttcagctctt tagcgatggc agcccacaga tcaacgtcaa agcccacata
     6481 tttatcgccc tgtttaaatt caaacggaac gaaggcggta tccgtcgcga caactaattt
     6541 tttatccgcg gcatgagaag aaaccgcaaa agccagggtc agtgcagcca gtgaaacttt
     6601 taatacagac ttcatagcat ttcctttttt ctatccacgg gacgatcccc tgcgtgacat
     6661 tcacatatat gaaaaaatcg tgccaatttt gcaactcatt gatttttcga gatgcggcaa
     6721 aaacgtgatg cacgatttat agggcaatac cctgaagatg caccattctg gggcaccaat
     6781 ctggtgcgct aaaattgtgc actcaacaca gtgattattt agcgtaaaac ctgatgaaaa
     6841 aacaatcttt atgtaacgat tgtgtgatga tgtggataca aaaaatttac ttaatcagct
     6901 ggggatagca gatggatgca ctaaataagt gcgttgaggt gggctgcaaa acaaaacggc
     6961 ctcctgtcag gaagccgctt ttatcgggta ctaaagttct gcaccatcag cgatggattt
     7021 attcgatgtt agactcgata aaccacagga atttatccag gtcgcgagac gcggcggtca
     7081 ggatatctgc ggtgtcgtca tctttcgctt cgccaatcgc tttgcgtacg tcattagcga
     7141 cgattgcgta acggtcagcc agttctttca ggtgatcctg aacgttgtgg atgtccagcg
     7201 ggtaactttt cagcggggtt ttgctgttga taacttgagt ggtccccaga gctacaccgc
     7261 ccagctgcac ggcacgttct gccatggtat ccagatgatc gatcagtgcg gtgcggaagc
     7321 catccagcat ttcatgtacg gcaatgaagt tagcgccgcg catgttccag tgcgcttgtt
     7381 tggtaatcaa agaaagatca ataaactgga taacctggcg attcagcaac tctactgttg
     7441 cttttttctc gctgtcggag acatcgttgc gggtataaag cagattggtc gcttttgatt
     7501 taactaattt agcggtactc ataatttcat atcctcttga tgttatgtcc cagtaattaa
     7561 cgagactaag tatagcaccg gctatgtgtt ccgctattct ggctgttcct atcacactaa
     7621 tagtggtaac aagcgtgaaa aacaaaacta ataaagattc aatgagttag atatatcgat
     7681 aagaacaatt cttatttatc tgctcgccag gaattttcgg aataaatatg cttttgcata
     7741 attcgatata taaatacggt gtagaggaaa agtagcgaga aattctgcat ggttatgcat
     7801 aaccatacag aaatgtaaat ttaattaatg tctaattctt ttattttgct ctctttgcgt
     7861 actgtcagcg tagaccccat tgaagcggcg atgatagcgc cgagcgccag cagctgtatg
     7921 ggtgtcagtg tttctccgag gaaaatcatc ccggaaacgg cagccagcgc cggttccatg
     7981 ctcatcagcg taccaaatgt ccgtgttggc aaacgggtga gggcaatcat ttccagcgaa
     8041 taaggcagag cggtcgagag aataacgaca gccagaccca atggaataac cgaccagtgc
     8101 cagagtgctt caccagcctg aagcgctcca attggcacga aaattaacgc tgcaatcaac
     8161 gaaccaattg ctaccgtcgc agggccatgt tccgctcctg cgcgttgccc acttaaaatg
     8221 taaatagccc aacaagcccc ggcccccagt gccagcgcac agccggttaa atcgacatgg
     8281 gaaacgtctt gccccagcgg tagcaggaac cacagaccaa gaaccgccag cacaacccag
     8341 acgaaatcta ccgggcgacg agaagagaac agcgccaccg ccagtggtcc ggtgaactcc
     8401 agcgccaccg caatacccag cggtactgtc tgaatagaaa gataaaaaag ataattcatc
     8461 ccacccagcg aaacgccgta aaacaacagc ggtaaccgtt gctctttggc aaagcgcagt
     8521 cgccatggct taaagaacgc gatgaggatc agcgttccta atgccagacg cagcgcagtg
     8581 acacccggtg cgctcaccag aggaaaaagt gacttagcta acgaggctcc accctgaata
     8641 gacgccatgg caacgagcaa tatgactatt ggtaaccaga ccggcatttt acgtaatgaa
     8701 ccaggcatcc tttctcccac aaatatctag acttaagtaa agcgtggagt gtactggata
     8761 tacccaatgc tggttgagca tttgttgaaa aaattttccc ccgttttgac taaaatgcgc
     8821 caggattgat ggaatcatta gtctggtgat taggaataat ctggatgaat gacagggaaa
     8881 acatgcgtaa tacttacgca gttctctgaa aaagtgattt aaattcagat ggatagcggt
     8941 gtatggaaac gttctgttac atgaaatggc ccgttagaca tcacaaatcg cgaagagttt
     9001 cccattaatt tttgatatat ttaaaactta ggacttattt gaatcacatt tgaggtggtt
     9061 atgaaaaaaa ttgcatgtct ttcagcactg gccgcagttc tggctttcac cgcaggtact
     9121 tccgtagctg cgacttctac cgtaactggc ggttacgcac agagcgacgc tcagggccaa
     9181 atgaacaaaa tgggcggttt caacctgaaa taccgctatg aagaagacaa cagcccgctg
     9241 ggtgtgatcg gttctttcac ttacaccgag aaaagccgta ctgcaagctc tggtgactac
     9301 aacaaaaacc agtactacgg catcactgct ggtccggctt accgcattaa cgactgggca
     9361 agcatctacg gtgtagtggg tgtgggttat ggtaaattcc agaccactga atacccgacc
     9421 tacaaacacg acaccagcga ctacggtttc tcctacggtg ctggtctgca gttcaacccg
     9481 atggaaaacg ttgctctgga cttctcttac gagcagagcc gtattcgtag cgttgacgta
     9541 ggcacctgga ttgccggtgt tggttaccgc ttctaatcac tttggtgata taaaaatccg
     9601 cctctcgggg cggatttttg tttttaaggt ttcgggtcga aaatatcggt tccgagatgg
     9661 ttgtaatcca ccttctgtaa ctggaagttg gtgatgtata tcggcccggc tttcttctca
     9721 gatataaacg ggtatttgat atttatttcc ttcgctttaa ttcccgtcca ctgcgagaaa
     9781 aagcctaaga agtcattggc tgagcggcgg gctttaatca cccgatgcgc tttatcgccg
     9841 ctggaaatga ccataaaagg cacctggaaa ttttgctgat atttatcatc atgggcaagg
     9901 tattgcacgt ctttgccgcg ctctttaaag gccagaccgt ggtcagaaaa gtaaaccaga
     9961 gagaagctgc tgccgctgtt gcgtaactga tcgtacagct tgcgcagtaa atcgtccgtt
    10021 tgcgtcatgg tatagagata gcacgacgtt tctttcgatt gcacaaaggt ttcgtatttg
    10081 ccttgtgtcc tgtcgcaggc ctgcggatgc gagcccatca gatgcagaac aatcagctgc
    10141 ggttgcgagt gctcttgcgc cagcacttga gcggtcatat ccagtaacgc ttcgtctttc
    10201 gtgtttttat ctgcttcaaa attaccttct ttcaggaagt acacttcatc tgcacgtttg
    10261 gcgatgctgg cgatagcagt atcgtattcg ccgatttggc cctggttgga aaaccaccag
    10321 gtctggaagc ccgcgcgatt tgccagggtg acaaagttat cctgaaactg tggtttgcca
    10381 tcgactacgc gattgagcgt taagccaagc gatttctgcg tggagccact ggcagcaatg
    10441 tagtcagcaa atatcaaacc gttaacgctg ctggcaaacg gggtattgtc ccagtgaccg
    10501 ccaaaggcac cgagggcatc gcgacgcgcg ctttcaccga tcactaccac ataggtctga
    10561 tacttcggct taacggcagt gaccgtccag gtatctttca ttccggaaag ttttgccatc
    10621 cgttcttgct cttcaatcac ctcgttatta ttgacgatga catccttagc aaaacgaaat
    10681 accggatagc cagtatcttt cagtttaaat acgccacccc aggccaggtt ttgcacggga
    10741 gcaacaaaaa atgtcgccac actgaatacc agacacaggc tgtcgaaggt attccagcgc
    10801 gctttttcat tctctttttt tcgcctgatt gctattacgc cgagcgcaaa aataaataag
    10861 ccgaccaggt agctgtacca cggaaaaatc gtcaggattt cggtcgactc ttccatattg
    10921 gtggagtgca atgccagcaa tgtattgaaa ttaggcgcgc cgtaggcctg agcaaaaggg
    10981 aaataacaag ccgccaccag cgaactgaca ccgaccagaa ctttttgtac gcgaggcaat
    11041 gttcgccata gcaaaagcaa aatagccgta aacgcagcgg tgtagagcaa actgaagggg
    11101 taacctaagc cgaggttaat taatagcgac tgtaaaaagt agaacgcagt ccacgggcta
    11161 aatacccggc tacgggtaac aagcgattct ttgagggtta aattcatatg ccactatcgt
    11221 aacaaacgcc atgtgcttac cctggcgtca agggtcaata cctgcaagag tgcgtgaaga
    11281 ggggaaaggg gtccgcatcc gcgagccgca atatacgcag ggctgcaaga agatagagcg
    11341 agccgccgtt caggtcaact actgacgaac aaatcttttg cgaaggggat tgcaaatccg
    11401 acaaaaagtg gtttttttca gaccaaaacg cgttatgcct gcataaaagc aggagtgaag
    11461 cggcgtaccg cgacgccgct atttatctga aggtttatct tgctgcggtt tgttgttgac
    11521 catatcgcac aacatagaga gcagcattaa ccgtacttta aagggagaat gactaaacac
    11581 gcgcatacac ctcttgaact cattcataag acctcctgac ttgctaatcc cgtcgatcct
    11641 tgagggatga ttgcattaca tacagatata gcacaggcta tattatatag ctattgctaa
    11701 aacgttaatt ttttgtacct tcgcaactct ggtttacaat gtgcgcacga aatgagaacg
    11761 ctatgtatgc gtcaccataa tgaggaagca caatgagtcg tcgcgcaggt acgccaatag
    11821 caaaaaaagt gacgcagtta gtgaacgtgg aagagcacgt tgaagggttc cgccaggtca
    11881 gagaggcgca tcggcgcgag cttattgatg attacgttga gctgatttct gacttgatca
    11941 gggaagtggg ggaagctcgt caggtggaca tggctgctcg tctgggagtt tcgcaaccga
    12001 cggtggctaa aatgcttaag cggctggcaa ccatggggct gattgaaatg atcccctggc
    12061 gtggcgtgtt tttaacggca gaaggagaga agctggcgca ggaaagccgc gagcggcatc
    12121 agatagtcga aaatttcttg ttggtgttgg gcgtcagtcc ggaaatcgcc cgtcgcgacg
    12181 cggaaggcat ggagcaccat gttagtgaag agacgctcga cgcttttcgt ttgtttaccc
    12241 agaaacacgg tgccaaatga gcctgccttt tttacgcacg ctgcaaggcg atcgtttttt
    12301 tcagttatta attcttgttg gtatcggatt aagctttttc gtgccctttg caccgaaatc
    12361 ctggcctgct gctatcgact ggcacaccat catcacctta agcggcctga tgctgctgac
    12421 caaaggtgtg gagttaagcg gttattttga tgtgctgggg cgcaaaatgg tgcgccgctt
    12481 tgctacggag cgtcggctgg cgatgtttat ggtgctggcg gcggcgctgc tttctacctt
    12541 tctgaccaac gatgtcgcgc tgtttattgt tgttccgctg actatcacgc taaaaagact
    12601 gtgtgagatc ccggttaatc ggctgattat ttttgaggcg ctggcagtca acgctggttc
    12661 gctactgacg ccaattggca acccgcaaaa tattcttatc tggggacgtt ctggtctttc
    12721 gtttgccgga tttattgccc aaatggcacc gatggctggc gcaatgatgc tgacactcct
    12781 gctgttgtgc tggtgttgtt tccctggaaa ggcgctgcaa taccatacgg gggtgcaaac
    12841 accggagtgg aaaccgcggc tggtgtggaa ttgtctgggg ctgtatatcg tctttctgac
    12901 ggcgctggag ttcaaacaag agctgtgggg actggtgatt gtggcggcgg gctttgtgct
    12961 gctggcgcgt cgcgtggtgt tgagtgtgga ctggacgttg ctgctggtgt ttatggcgat
    13021 gtttatcgac gtccatttac tgacccagct tccagcgttg caaggcgtgt tgggtaacgt
    13081 gagtcattta tctgaacccg ggttatggtt aacggcagtc ggtttatcgc aggtgatcag
    13141 taacgtgccg agtaccattt tgctgctcaa ttatgtacct ccgtctttat tactggcatg
    13201 ggcggtaaac gtggggggct ttggtttatt accaggatcg ctggcaaatt tgattgcgct
    13261 acgtatggcg aacgatcgcc gcatctggtg gcgtttccat ctctattcaa taccgatgct
    13321 gttgtgggcg gcgctggtgg gatacgtttt gttagttatg atcccggcct ggtaaggtct
    13381 ggcaaataaa aaaggcggat tatgagtccg cctttgtttt acgttttgac gattaattca
    13441 ggcgaaccgg catcccggaa cgatttttga tcgcttcatc aagaacaacc tggtcaacat
    13501 ctggctgacc ggtcactgtc tgcacgctct tcgtcagggt aattggcacg atttcctgac
    13561 cttcaaactg ggcttcggtg gtagacagcg ggttatggac ttcaatataa cggctgccgt
    13621 ctggctcggt ggtcgctttt accggctcat caataaactg tacgcgggta ccgaccggta
    13681 ctttctcgaa caggaatttg atgtcttcgt tacgcagacg cacacaacca tgacttacac
    13741 gcaggccgat accgaagttg gcgttggtgc catggatagc atacaggcga ccgatgtaga
    13801 gtgcatacag acccatcggg ttatccggac ctgccggaac gacagccggt agcggttcgc
    13861 ccgcagcgcg gtactctgcg tgcattttgg cggtcggcgt ccaggtcggg cctgctttct
    13921 tatgctcaac tttggtggtc cagttgatag gcgtatcttt gcctaactga ccaatgccga
    13981 tcggcagcac gataacggtg ttggtccctt tcggatagta atacagacgc atctctgcac
    14041 tgttaatgac gatgccttca tgaacggtat ccggcaggat cagctgctgc ggaatgttca
    14101 gtacagtacc gcctttcggc aggaaggtat ccacacccgg gttcgcttcc atcatattgg
    14161 aaagccccat ctggtactcc gcggcaaaat actccagcgg ctgagtgtta ccttcaggaa
    14221 tggtgatcac ctgattctga ccaaccaggc gactcccgtc ggttggcaga ggataagtta
    14281 ccgcagaggc ggtactgcaa aagccgacaa cagcgaaggc cgctgcgaat aatgttttca
    14341 atttcatatt catgttaagc gagattttgt gcctggcagg ccattgggtt gagaatatta
    14401 gagtattgga agcgcattat aagttcattc cagctcacag tgaaatcaga tgtgtacgaa
    14461 atcacatttt ttgcctttgg cttgagtgta gaccttaagc gaggagcagg atcttctttc
    14521 agacttatgg cataatgcgc ggtttgtcat atctcttttc aggatacgcc tgtgttagtt
    14581 tccagtaacg tcaccatgca gttcggcagt aagccgttgt ttgaaaacat ttccgtcaaa
    14641 ttcggcggcg gcaaccgtta cggcctgatt ggcgcgaacg gtagtggtaa atccaccttt
    14701 atgaagattc tcggcggcga ccttgagccg acgctgggta acgtttccct cgatcccaac
    14761 gagcgcattg gtaagctgcg tcaggatcag tttgcctttg aagagttcac tgtgctggat
    14821 acggtgatca tggggcataa agagttgtgg gaagtgaagc aggagcgcga ccgcatctat
    14881 gctttgccgg aaatgagtga agaagacggc tataaagtgg ccgatctgga agtgaaatac
    14941 ggcgaaatgg acggttactc tgcggaagct cgcgccggtg aactgttgct tggcgtggga
    15001 attccagtgg aacagcacta cggcccgatg agtgaagttg ctcctggctg gaagctgcgt
    15061 gtgcttctgg cgcaggcgct gtttgctgag ccggatattc tcctgctcga cgaaccgacc
    15121 aacaacctcg acatcgacac cattcgctgg ctggaacagg tgctgaacga gcgtgacagc
    15181 accatgatca tcatctcgca cgaccgtcac ttccttaaca tggtctgtac ccacatggcg
    15241 gatctggatt acggcgagct gcgcgtttat ccgggtaact acgatgagta catgacggcg
    15301 gcgacccagg cgcgtgaacg tctgctggcc gataacgcca agaagaaagc gcagattgct
    15361 gagttgcaat ctttcgttag ccgctttagc gccaacgcct cgaaatctcg ccaggcaact
    15421 tcgcgcgcgc gccagattga taaaatcaaa ctggaagagg tgaaagcctc cagccgtcag
    15481 aacccgttta tccgttttga acaggataag aaactgttcc gtaacgcgct ggaagtggaa
    15541 ggtctgacca aagggtttga taacggtcca ctgtttaaaa atctcaacct gctgctggag
    15601 gtcggtgaaa aacttgcggt actgggtacc aacggcgtcg gtaaatcaac gctgctgaaa
    15661 acgctggtgg gcgatctgca accggacagc ggcaccgtaa aatggtctga aaacgcgcgc
    15721 attggttact acgcccagga ccacgaatat gagtttgaaa atgatctgac cgtgttcgaa
    15781 tggatgagtc agtggaagca ggaaggcgat gacgagcagg cggtacgcag cattctcggt
    15841 cgtttgctgt tcagccagga cgacatcaaa aagccagcta aagtgctttc cggtggggaa
    15901 aaagggcgga tgctgtttgg taagttaatg atgcagaagc cgaacattct gatcatggac
    15961 gaaccgacca accacctgga tatggaatcc atcgagtcac tgaacatggc gctggaactg
    16021 tatcagggca cgctgatctt tgtttcacac gaccgtgagt tcgtaagctc cctggcgacc
    16081 cgcattctgg aaatcacccc ggaacgcgtg atcgacttta gcggtaatta cgaagattac
    16141 ctgcgtagta aagggatcga gtaagtgatt atccgtcacc ccgatactgt cggggtgacg
    16201 acggctgatg acaaatgcaa aatagcctga tgcgctacgc ttatcaggcc tacgcagtac
    16261 ctgtaatata ttgaatctgc aggactttgt aggccggata aggcgttaac gccgcatcag
    16321 gcattaagaa agcgcacttg tcagcaatct gagtaacgtc gaatcgattt tcttaatccc
    16381 acatcaattc acttccatta actctcaaac atccatgccc aacgctcgct taccgtgaat
    16441 gttgaggtcg gcaagcgtaa agcgtccttc ccagtttgtt tcatagtcct cgcgcgggaa
    16501 gtcgcccggc gatgcgcctt tttccagtgc ggccttcacc ttgtgcgcgt aggcaagatt
    16561 tttctcgcac atcggtgccg caggaatgta catcacgttg ccccaaccct gttgattttc
    16621 aacgggggca acggaatgga tgacgtcgca gtgccaccag acggagtctc cggcctcgag
    16681 ttttggaatg ctggttaacg cctcaattaa cagtggatgc cattgctctg ataccggcaa
    16741 cactcttccg ggcgctacgc cgcacagttc atcctccggc acatcatcaa gcagcggacg
    16801 taacagtacg tacgccatgg cttcaggaat gggcacgacg tgcagcaacc cctgaccagg
    16861 cagcatatcc gagagcgcag tccagccctg gaatgtccga aacacggaac atttggtggt
    16921 gttgtccacc gtgtactctt caacttccgt acgatgtgcc gcatgccagg gatcgtattt
    16981 cgccagattg ccattaaaga cgttggcgaa aacgtgctga tacgctggaa gcagccagcg
    17041 ttccagcgcc ccggagtcgg tatgcgctcc aagacctttg gaggtcgttc cgggcggaag
    17101 gcggcggata cggtcagggt agatgacgct cacatccggg ttaaaccatt gctttccatc
    17161 actttcaaat gtccacagac gattgagaaa cgactgcgca ttcgccattt cttcactctg
    17221 gcgggcctgc atttgcgcct gcgaccagta gatggggtaa atctcgggac gtgaagcgct
    17281 gagcgtcccg aagaaattat cgccggggcc tttgtagacc tcgtcaaagc ggttgcggtt
    17341 cagatagtcc agcatcgact gatcccagcc tagcgcttgt tcgcggggga aatggccttt
    17401 tatcaccgca caaccgcgac gtttaatctg ttcgcgctgc tctgcagtaa catgacctgc
    17461 tttgatatcg gcataagaca gcaccggcca gacggcatcg ccctgtgctt tgagtgcgtt
    17521 gatttcagcc actcgcgtgg caatgtcatc gcttagctga ttaaagatct gctggacatc
    17581 gccaagctgc gcccgcagcg cgtgcttcat ctgacggata gctgctttgt gatcggcagg
    17641 caatgtgtcg ctggtaaaag tagaagccat aaccaccttg cattaacttt cattcgaaac
    17701 tatgctaatt tgcatgtgac aatacaagtt ataaataagt taatgcaagt ttaatgattt
    17761 gatgtgatgt acaggatgta aaaagaaggg aaaaccggag cgcatggctc cggtagagag
    17821 gtcagctgtt aaaaggggat gtgttatcca gcacagcctg aatcacattc agcgcgcctt
    17881 catgattatt atcatcggta gcgtaacggg cgatttgttt aatgttttcc gcagcattgc
    17941 ccatcgcaaa ggaataacgc gccattttca gcatctccgc atcgttaccg ctgtcgccaa
    18001 tcgctaccac attttgcggt gacagatccc agcgtttcag taaccgcgaa ataccgtttg
    18061 ctttatgtag accgggaata atcaggtcga taaagccaaa accactggtg acgggtttca
    18121 taatgccatc cagcgctatg tgtagtttgt cgatcactaa cgggatttgt tcatccggca
    18181 ggttgagcga aaacttgaac agtacgtcgt caatctcctg ataatctttt acaggtttca
    18241 ggcgatggta gtgttttgcc atcagtgcga caaatgcttc gggggcattt tcgctgacat
    18301 atgcactttg cagaccgcag gcgacaaaat tgagttgctt atcttttagc aactcgccaa
    18361 taacaatccg cgattcatgt cgggtcagtt cgccgtggaa cagttgcttg ccatgttcgt
    18421 aaaccagtgc gccgttttcc gcgacaaaag agatctcatc ctttagctca ggaaagaacg
    18481 aaataagctg gtaatactga ttaccgctgg cgacaacgaa cttaatgccg cgctttttca
    18541 gttcctgata ttgcgccata aaacgtggtt ggttgtacgt tttggcgtcg ttaagaaaag
    18601 taccgtccat atctgtgacg ataactttta cgctcatagg tgtgctcctg gctcgaaaat
    18661 gaaaccgtaa cagtgtaata acaatgtgac gcagagcaca aattatattt cgaatgaaag
    18721 taaggatgaa attgatgatg tgaatgattt agcccggcga cgccgcagcc gggccgaaga
    18781 gattacagca tatgttcagt acgggcgatg atatcgtcct gagcgtctgg agagagcgcg
    18841 gtgaagaagg cggaataacc cgcgacacgc actaccagat cgcggtactg atccggatgt
    18901 tttttcgcct ccagcagcgt ttcgcgggaa acgatgttgt actgaatatg ccagccttta
    18961 tgcacttcaa agaaggtacg cagcaggatc atcagtttct gcttgtcaga ttcgttctcc
    19021 agcgttgccg gattcagttt ctggttgagc aacacgccgc cgagaatcgc tgccgtaggc
    19081 agtttaccca ctgagccaat gaccgcagta gggccgagat ggtcagtacc agaggccgga
    19141 ctagcgcctt ctgccagcgg ggtgtgggct ttacgcccgt ccggcgttgc catagtttgc
    19201 gcgccaaacg gtacgttagc ggagatagac gacgtacccg cgtaatagtt gccgccaacc
    19261 ggaccacgac cgtagcgtgg gttatggtac tgtttcagtt cgtcgatata ggtctgataa
    19321 gcgcgagcca gcagcgtatc gacggtatca tcgtcgttgc cgtacttcgg cgcaccgtta
    19381 atcagccgct gacgcagttg ctcatgagtc aggccatcga agtcatccgc cagtgcggca
    19441 gccagctgtt gctgaccaat cgcgccctgt tcaaacacca gtttcttcac tgccgccagg
    19501 ctgttgccga ggttggcaat accgacctgc aggccagaaa cccagtcata tttcgcgccg
    19561 ccttgcttga tacttttcgc tcgctcaata cagtcatcca ccagcgccga gcagagaata
    19621 tcgtgcacgt tctcttccag catggtgtcg acgacatatt cgatttcgat tgatttacgc
    19681 gtgtagtaac ggatttgcgt atcccacgcg tccatcactt catcgaagtt gttgaagtta
    19741 cctgccgaca acgctttttc ttgtggcagg aacactttgc cgctggtggc atcacgcccg
    19801 ccttccagcg ccgccagcat cacgcgggcg aagttgataa agctcatgcc ggtacagcga
    19861 tagccccatt tgccaccgac ggcggtttct atacaaccaa tcgctgcgta gtcataagcg
    19921 tcctgcggtt caataccgag tttaataaat tccgggatca cgatttcgtc gttgttgaat
    19981 gccggcatcc cgaagccgca gcggatcaca tgtacgcagg cgtcgaggaa atcgttactc
    20041 attcctgcat ggtaacgcac gctgaggtta ggctgagtgg aacgcaggcg accgcaggat
    20101 tcgaggatcg cgtaagagag tggattcacc gcgtccattg gttgaccatc aaccagattt
    20161 tgtccgccaa tggtgacgtt ctgatacagc ggacttcccg cagaggcttt tgagtgggag
    20221 ccggagcgga ttttgttcac ttccagcaat ttcaaccagc agctatgcag caattcgatg
    20281 gcgtgttcgc gatccagcgt ctggttcagt tcaacgtcgc ggcgatagta cgggtagaga
    20341 tactgatcca tacgaccaaa cgacaccgag tgaccgttag attcaatctg caaaatcaac
    20401 tggatgaagt aacacagttg cagcgcctgc cagaaagtct gcggcggctg gtgggcgata
    20461 agatcgcagt tttctgccat cgccagcagt tcatcgcgac ggctttcgcg ggtttcggtc
    20521 gcggccattg cacgcgccag ggcagcgaaa cgttcaatgt gttcactgac tgccaccagc
    20581 atgatatcaa tcgctttcag gaactgttcg ccgtgcaaat cttccagcac cgtcagattg
    20641 atgcgcgagc gacgttccgc tactttctca cgcagaccat caagcccttt ttccagcagc
    20701 agcgggaaat tgaccgccag gtgcgcatcg ccggaggtca tattgccttc cgctttaatg
    20761 atgccggtcg ccagcagacc tttttgctca tcggtaaaca tgccatagca gcgatcctgt
    20821 accgtctgac cgcgccacca cgggcacact tcatgcagaa cacgtttgtt ctcttcgctc
    20881 accgcaaagc cagcaccggg acgatctgcc agatcatcaa tctctttttc gatccacgag
    20941 acagtatatt ccgggaagat cggcgcggcg cgaacttcgc ttgcctggtt gccaatgatc
    21001 aactcatcgt gtttgatcca gatagtgcga ttcgccaggt gatgcgccag tgccagcgcg
    21061 cgacgtaccg ggatcggctt atcgagatgt tgttgataca tctcggtata gtgctgcgcg
    21121 cgctcggtac agactggcgg cttcacaata tgcaccagcg catttttgtg cgctttaatg
    21181 cggtcgctga gcgtgtccag tttcagtgtg gtcatggttg ttatcctcgt aaggtcgcgg
    21241 ttaacccttt ctggcaggca tactgctggg caaaatcgag cagctctggc gcatcaagcg
    21301 gtttttccgg ggcgtcatag ggcagattaa gtaagtgata tttgttgatg cccagcgtgt
    21361 ggtagggcag aaaatgaatt tcgccaacgt gcagctcgtc ggcggcaaaa tcggtaatgg
    21421 cttttacaga ggtttcgtcg gcattaaagc cctgaatcag cggcacgcgg ataatgattt
    21481 ttttgcccgc tgcggcgagt tttttcaggt tatccagcac cctggcggcg ttaccgtcgg
    21541 tccactgttt aaacggcgcg tcggcaacgt gttttaaatc ggcaagaaac agatcgatat
    21601 agggcagaga aggggcgata tatttccacg gcacatgcag acaggtttct accgcagtat
    21661 gaatgcctgc ctcgtggcag gcttgcagca gcgccgtcgc catttccggc tgcatgaagg
    21721 gttcaccacc cgaaagcgtt aagccgccgc cgctgcgatc gtaaaacggt ttatcgcgca
    21781 gaacggtcgc catgatctcc tcaacgcttt tcacttcacc acacacggtt aacgcctgtg
    21841 tcggacagca gtcggttaac gccgtcagat gctccggggt taacttttcc cgatgaataa
    21901 gcaaaccatt cagcgcgcgc tcaatcactt ccggcgcggc cttagcgcac agctcgcagc
    21961 cttccagaca cagtcgtgcg tcatacagca gatcctgcgt acgggcgcgg ctttccgggt
    22021 tctgacacca gcggcagccc agcgaacagc ctttaagaaa tacgaccgtg cggataccgg
    22081 ggccatcatg ggtcgagtag cgctgaatat tgaaaatcat agttgcctct ctgtttcgtt
    22141 caaacattaa aatactttcg aatgaaagtt agattgatgt gcgtcaactg ttcagagagt
    22201 tttcccgtga tagtctacat tcagacaaaa agtacatttt gaggatggtt atggaactgt
    22261 atctggatgc ttcagacgtt gttgcggtga aggcgctgtc acgtattttt ccgctggcgg
    22321 gtgtgaccac taacccaagc attatcgccg cgggtaaaaa accgctggat gttgtgcttc
    22381 cgcaacttca tgaagcgatg ggcggtcagg ggcgtctgtt tgcccaggta atggctacca
    22441 ctgctgaagg gatggtgaat gacgcgctta agctgcgttc tattattgcg gatatcgtgg
    22501 tgaaagttcc ggtgacagcc gaggggctgg cagcgattaa gatgttaaaa gcggaaggga
    22561 ttccgacgct gggaaccgcg gtatatggcg cggcacaagg gctgctgtcg gcgctggcag
    22621 gtgcggaata tgttgcgcct tacgttaatc gtattgatgc tcagggcggt agcggcattc
    22681 agactgtgac cgacttacac cagttattga aaatgcatgc gccgcaggcg aaagtgctgg
    22741 cagcgagttt caaaaccccg cgtcaggcgc tggactgctt actggccgga tgtgaatcaa
    22801 ttactctgcc actggatgtg gcacaacaga tgattagcta tccggcggtg gaagccgctg
    22861 tgacgaagtt tgagcaggac tggcagggag cgtttggcag aacgtcgatt taaccggacg
    22921 ttctgcatcc tcataaattg ctgatgacgt ggcggagtgc accgatgcct gccgcgcttg
    22981 gaaatctgtg cccgaaccgt aggccagata aggcgttcac gccgcatctg gcgtctggag
    23041 caaatgcctg atgcgacgct tgtcgcgtct tatcaggctt ggaggtggca attactgccc
    23101 gcacacctca caccccggat tgcgcatcag tttcatttcg cgaaactgac aggtcatcgc
    23161 atcgtacatg acgattttcc cgctggccgg tttgccataa cctgccagca gtttgatcgc
    23221 ttccattgcc tgcaacgaac caattacgcc tatcaacggt gccatcacgc ctgcttctac
    23281 gcaggttaac gcgttctcgc caaacaaacg gctaaggcag cgatagcacg gttcgccgtc
    23341 ctgataagtg aagaccgtga tttgcccttc catacgaatt gccgcgccgg aaaccagcgg
    23401 caccttcgcg gcaaaacagc ctgcgttcag ttgattacgt accgcaacgt tatccgtaca
    23461 gtcgagcacc agatcgtgtt cagcaatcaa tgctgcaagt tctgcgtcat ctagcagtgc
    23521 attgactggc gtaatcgcga tatgtgggtt gatccgcgtc agggcgtcac gggcggattc
    23581 caccttcggt tgcccgaccg tggcatcact gtgcagtgtc tggcgttgca gattcgagag
    23641 cgaaaccgtg tcgaagtcga gcagcgtcag gttgccgaca ccggcgcttg ccagatactg
    23701 cgaagctgcg cagccgagtc caccgaggcc gactaccagc acacgagaat cttttagcgc
    23761 ctcctgaccg tcaaaatcaa aaccgcgcag aatgatttgc cggttgtagc gcagcatctc
    23821 ctgatcgctg agttccgcca ttacaggcct ccgaacaacg cgttaaacgg ttctacttcc
    23881 acccattcgc gcacgtccac attgccgcga tcgcgttcca gcacgataaa gcagttgccg
    23941 agactaaagg agctaaatat atgtgaaccc tgatgtccgg tggtcgtcac ttcaagttca
    24001 ccgtcggcgt tgcgttgcag cacgccgcgc tggaaatcaa gacgtccagg cgtttttttc
    24061 aggcgggacg ctgtgcgtac gcgctggcgc gcgggcaggc cgctggcggt gttgccgctt
    24121 agttttgcca gcaaaggctg taccagttga tagaaggtca gcgtcgctga aaccgggttg
    24181 cccggcaggc cgcagaacca gctattgctg agtttaccga acgcgaacgg tttacctggt
    24241 ttaatcgcca gcttccagaa ggcgatctcc cccagctctt caagaatcat tttggtgtaa
    24301 tccgcttcac ccactgaaac accgccggaa ctgatcacta catccgcctg gctgtcggct
    24361 tcaataaatg cggcgcgcag ggcatgtgga tcgtcgcgga taatccctaa gttgattacc
    24421 tcgcaaccca gctgttctaa catcaggtgt acggcgagac ggttggtgtc gtagatttgg
    24481 ccgtcgccca gcggctgacc gggcaactgg agttcatcac cggtagaaaa aagcgctaca
    24541 cgcactttac gaatcaccgg aacttcggca atccccagtg aagcaattac tggcagctct
    24601 gcggtagtca ggcgagttcc cgccgggaaa acaaccgcac ctgcagagat atcttcaccg
    24661 cgacggcgaa tattttgccc gctacgcact tcagcagtaa aacgcacgcc attgtccgtt
    24721 tgttcagtct gctcctgcat caccaccgct tcgcagcctt ccggcaccgg cgcaccggtc
    24781 ataatacgaa tgcaggtacc cgcaggccat tcaccatggt atggctgacc ggcaaaggat
    24841 ttaccggcaa cgggcagcgg ttgcccggag gcaatatcgg ctaaacgcac cgcgtagccg
    24901 tccatagcgg agttatcaaa ccccgggaca tccagtggcg aaacgacatc gctcgccaga
    24961 atacgaccaa aacactgaac cagtggcagc gtttcctggg tggtcagtgg ggtaacgcga
    25021 gaaagcatct cattaagcgc ggtgtcgagc gacatcaatc cggtggtaaa ttccatgaaa
    25081 acactcctgc ggaggcaaaa tcgaatttgc ctattatgtc agaaaaacgc catagactgt
    25141 atgccacctc gggcgtagcg ttggctcctg cctttacatg ccatatccat ctttctatat
    25201 tcaaaaattg aatgagtaat tcataaaaat tctgatattt atagcaaaag tggcgagcca
    25261 cccttaatgg acgaatacta tgggcaaagc agtcattgca attcatggtg gcgcaggtgc
    25321 aattagccgc gcgcagatga gtctgcaaca ggaattacgc tacatcgagg cgttgtctgc
    25381 cattgttgaa accgggcaga aaatgctgga agccggcgaa agtgcgctgg atgtggtgac
    25441 ggaagcggtg cgtctgctgg aagagtgtcc actgtttaac gccggaattg gcgctgtctt
    25501 tacgcgtgat gaaacccatg aactggacgc ctgtgtgatg gatggtaaca ccctgaaagc
    25561 cggtgcggtg gcgggcgtta gtcatctgcg taatccggtt cttgccgccc ggctggtgat
    25621 ggagcaaagc ccgcatgtga taatgattgg cgaaggggca gaaaattttg cgtttgctca
    25681 tggcatggag cgcgtctcac cggagatttt ctccacgcct ttgcgttatg aacaactact
    25741 ggcagcgcgc gaggaagggg caacagtcct cgaccatagc ggtgcgccac tggatgaaaa
    25801 acagaaaatg ggcaccgtgg gggccgtggc gttggattta gacggcaatc tggcggcagc
    25861 cacgtccaca ggcggaatga ccaataaatt acccggacga gttggcgata gtcccttagt
    25921 gggtgccgga tgctacgcca ataacgccag tgtggcggtt tcttgtaccg gcacaggcga
    25981 agtcttcatc cgcgcgctgg cggcatatgg catcgccgcg ttaatggatt acggcggatt
    26041 aagtctcgcg gaagcctgcg agcgggtagt aatggaaaaa ctccctgcgc ttggcggtag
    26101 cggtggctta atcgctatcg accatgaagg gaatgtcgcg ctaccgttta acaccgaagg
    26161 aatgtatcgc gcctggggct acgcaggcga tacgccaacc accggtatct accgtgaaaa
    26221 aggggacacc gttgccacac agtgatgaac ttgatgccgg taatgtgctg gcggttgaaa
    26281 atcttaatat tgcctttatg caggaccagc agaaaatagc tgcggtccgc aatctctctt
    26341 ttagtctgca acgcggtgag acgctggcaa ttgtaggcga atccggctcc ggtaagtcag
    26401 tgactgcgct ggcattgatg cgtctgttgg aacaggcggg cggtttagta cagtgcgata
    26461 aaatgctgtt gcggcggcgc agtcgtgatg tgattgaact tagcgagcag agcgctgcac
    26521 aaatgcgcca tgtgcgcggt gcggatatgg cgatgatatt tcaggagccg atgacatcgc
    26581 tgaacccagt atttactgtg ggtgaacaga ttgccgaatc aattcgtctg catcagaacg
    26641 ccagtcgtga agaagcgatg gtcgaggcga agcggatgct ggatcaggta cgcattccgg
    26701 aggcacaaac cattctttca cgttattcgc atcaactctc tggcgggatg cgccagcgag
    26761 tgatgattgc gatggcgctg tcatgccgcc cggcggtgct gattgccgat gagccaacca
    26821 ccgcgctgga tgtcactatt caggcgcaga tcctgcaatt aatcaaagta ttgcaaaaag
    26881 agatgtcgat gggcgttatc tttatcactc acgatatggg cgtggtggca gagattgccg
    26941 atcgggtact ggtgatgtat cagggcgagg cggtggaaac gggtaccgtc gaacagattt
    27001 ttcatgcacc gcaacatcct tacacccgtg cgctgttagc tgctgttccg caacttggtg
    27061 cgatgaaagg gttagattat ccccgacgtt tcccattgat atcgcttgaa catccagcga
    27121 aacaggaacc ccccatcgag cagaaaacgg tggtggatgg cgaacctgtt ttacgggtgc
    27181 gtaatcttgt cacccgtttc cctttgcgca gcggtttgtt gaatcgcgta acgcgggaag
    27241 tgcatgccgt tgagaaagtc agttttgatc tctggcctgg cgaaacgcta tcgctggtgg
    27301 gcgagtctgg cagcggtaaa tccactaccg ggcgggcgtt gctgcgcctg gtcgaatcgc
    27361 agggcggcga aattatcttt aacggtcagc gaatcgatac cttgtcacct ggcaaacttc
    27421 aggcattgcg ccgggatatt cagtttattt ttcaggaccc ttacgcttcg ctggacccac
    27481 gtcagaccat cggtgattcg attatcgaac cgctgcgtgt acacggttta ttgccaggta
    27541 aagacgcggc tgcacgcgtt gcgtggttgc tggagcgcgt gggcctgtta cctgaacatg
    27601 cctggcgtta cccgcatgag ttttccggcg gtcagcgcca gcgcatctgc attgctcgcg
    27661 cgttggcatt gaatccaaaa gtgatcattg ccgacgaagc cgtttcggcg ctggatgttt
    27721 ctattcgcgg gcagattatc aacttgttgc tcgatctcca gcgtgatttc ggcattgcgt
    27781 atctgtttat ctcccacgat atggctgtgg tagagcggat tagtcatcgt gtggcggtga
    27841 tgtatctcgg gcaaattgtt gaaattggtt cacggtgcgc ggtcttcgaa aacccgcagc
    27901 atccttatac gcgtaaatta ctggcggcag ttccggtcgc tgaaccgtcc cgacaacgac
    27961 cgcagcgtgt actgctgtcg gacgatcttc ccagcaatat tcatctgcgt ggcgaagagg
    28021 tggcagccgt ctcgttgcaa tgcgtcgggc cggggcatta cgtcgcacaa ccacaatcag
    28081 aatacgcatt catgcgtaga taacattcag gcggagaata aaatggcaag agctgtacac
    28141 cgtagtgggt tagtggcgct gggcattgcg acagcgttga tggcatcttg tgcattcgct
    28201 gccaaagatg tggtggtggc ggtaggatcg aatttcacca cgctcgatcc gtatgacgcg
    28261 aatgacacgt tatctcaggc cgtagcgaaa tcgttttacc aggggctgtt cggtctggat
    28321 aaagagatga aactgaaaaa cgtgctggcg gagagttata ccgtttccga tgacggcatt
    28381 acttacaccg tgaaattgcg ggaaggaatt aaattccagg atggcaccga tttcaacgca
    28441 gtggcggtaa aagcgaatct ggaccgggcc agcgatccgg cgaatcatct taaacgctat
    28501 aacctgtata agaatattgc caaaacggag gcgatcgatc cgacaacggt aaagattacc
    28561 ctcaaacagc cgttctcagc gtttattaat attcttgccc atccggcgac cgcgatgatt
    28621 tcaccgacag cgctggaaaa atatggcaag gagattggtt ttcatccggt gggaaccgga
    28681 ccgtatgaac tggatacctg gaatcagacc gattttgtga aggtgaaaaa attcgcgggt
    28741 tactggcagc caggattgcc caaactggac agcataacct ggcgtccggt ggcggataac
    28801 aacacccgcg cggcaatgct gcaaaccggt gaagcgcagt ttgctttccc cattccctac
    28861 gagcaggcca cactgctgga gaaaaacaaa aatatcgagt tgatggccag tccgtcaatt
    28921 atgcagcgtt atatcagtat gaacgtgacg caaaagccgt tcgataaccc gaaggtccgt
    28981 gaggcgctga attacgccat taaccgtccg gcgctggtga aagttgcctt tgcgggctat
    29041 gcaactccag ctactggtgt ggtaccgcca agtatcgcct acgcgcaaag ttataaaccg
    29101 tggccttacg atccagtgaa agcgcgcgaa ttactgaaag aggcgggata tcccaacggt
    29161 ttcagtacca cgctgtggtc gtcacataac cacagcaccg cgcagaaagt gctgcaattt
    29221 acccagcagc agttagcgca ggtcgggatt aaagcccagg tgaccgcgat ggatgccgga
    29281 cagcgggcgg cagaagttga aggtaaaggg caaaaagaga gtggcgtgcg gatgttctac
    29341 actggctggt cggcttcaac cggcgaagcc gactgggcac tatcgccgct gtttgcctcg
    29401 caaaactggc caccgacgct gtttaatacc gcgttttaca gcaataaaca ggtggatgac
    29461 ttcctggctc aggcactgaa aacgaatgat ccggcggaaa agacccgctt atataaggcg
    29521 gcgcaggata tcatctggca agaatcgccg tggatcccgc tggtggtaga aaaactggtg
    29581 tcggcacaca gtaaaaacct gaccggtttt tggatcatgc cagataccgg cttcagcttt
    29641 gaagacgcgg atttgcaata agcaacgcag ggagtggaat gcttaattac gttatcaaac
    29701 gattactggg gttgattccg acgctgttta tcgtctcggt gctggtgttt ttatttgtcc
    29761 acatgctgcc cggcgatccg gcgcgattga ttgccgggcc cgaagctgat gcgcaggtta
    29821 tagaactggt gcgtcagcag ctggggttgg atcagccgct gtatcgccag ttctggcact
    29881 atatcagcaa tgctgtgcag ggggattttg gcctgtcgat ggtgtcgcgt cgtccggttg
    29941 ccgatgagat tgccagccgc tttatgccaa cgctatggct gaccataacc agtatggtct
    30001 gggcggtgat atttggtatg gcggcgggaa ttatcgccgc cgtctggcgt aatcgttggc
    30061 cggatcgatt gagtatgacc attgcggtgt cggggatctc gtttccggca tttgcgctgg
    30121 ggatgttttt aattcaggta ttctccgttg aactgggctg gctgcctact gtgggagcag
    30181 acagttggca gcactacatt ttaccctccc tgacgctcgg cgcggcagtg gccgccgtga
    30241 tggcgcgctt tacccgtgcg tcatttgtcg acgttttaag cgaagattat atgcgtaccg
    30301 cgagggcgaa aggggtgagc gaaacctggg ttgtcctcaa acacgggcta cgcaacgcga
    30361 tgatcccggt agtgaccatg atgggcttac agtttggctt tttgctcggt ggttctatcg
    30421 tggtggagaa agttttcaac tggcccggac ttggacgctt actcgttgac tctgtagaaa
    30481 tgcgcgatta cccggtgatt caggcggaaa ttctgctttt ctcgctggaa tttattctta
    30541 tcaacttagt ggtggatgtg ctttacgccg ccattaaccc ggctatcagg tacaagtaag
    30601 gatgcgacta tttaactggc gacgtcaggc ggtgttaaac gccatgccac tggtcaaacc
    30661 tgaccaggta cgtacaccgt ggcatgaatt ctggcgacga tttcgccgtc agcatatggc
    30721 gatgaccgcc gcattattcg ttattttatt gattgtggtg gccatttttg cacgctggat
    30781 cgctccctat gacgccgaaa attattttga ttatgacaat ctgaataacg gaccttcttt
    30841 gcagcactgg tttggcgtcg attcactggg gcgcgacatt ttcagccgtg tcctggttgg
    30901 tgcgcaaatc tcgctggcgg cgggcgtgtt tgccgtgttt atcggtgtgg cgatagggac
    30961 gttgctgggc ttgttggctg gatattatga aggctggtgg gatcggctga tcatgcgcat
    31021 ttgcgatgtg ctgtttgcct tcccgggtat tttactggcg atcgctgttg ttgcggtgtt
    31081 gggaagcggc attgctaacg tgattatcgc agtcgccatt ttttccatcc ccgcgtttgc
    31141 ccgcctggtg cgcggcaaca cgctggtgtt gaaacagcaa acctttattg agtcagcacg
    31201 cagtattggt gccagcgata tgaccatttt attgcgtcat atcctgcctg ggaccgtctc
    31261 ttctatcgtg gtgtttttca ccatgcgcat tggcacctcg attatctctg ccgccagcct
    31321 gtcatttctc ggcctcggtg cgcagccgcc gacaccagag tggggagcaa tgctcaatga
    31381 ggcacgagcg gatatggtta tcgcgccgca tgtcgctgtt tttccggccc tggctatttt
    31441 tctgaccgta ctggcgttca atttgttggg cgatggttta cgcgatgcgc tggatccgaa
    31501 aattaaagga tagttacgtt tgaatattgc ttgaaagggt aatcacctca caggaaatta
    31561 ttgccctaag caagtgttgt aactttctgc tgattttgta gaatcgggta atttggttaa
    31621 aaagccgcag caagggacta tttttgcagc ggcacagcgt tcagatagtt attttgttaa
    31681 atgtattaac atgctgagtt tatacgaaaa gataaagata aggctgataa ttttattttt
    31741 attggcagca ctgtcattta ttggtctttt tttcatgatt aactatcaac tggtatcgga
    31801 acgcgcggta aaacgtgccg atagccgctt tgaacttatt cagaaaaacg ttggctattt
    31861 ctttaaagat attgaacgtt cggccctgac attaaaggac tcactgtatt tattaaaaaa
    31921 tacagaggag attcaacgcg ccgtgattct gaaaatggaa atgatgccat ttttagactc
    31981 ggtgggactg gtacttgatg ataataaata ttatctcttt tcgcggaggg cgaatgataa
    32041 aatcgttgtt tatcatcagg aacaagtaaa tggaccgctt gtcgacgagt cagggcgggt
    32101 tatttttgcc gattttaacc catcgaaacg accgtggtcg gtggcttcag atgactctaa
    32161 caacagctgg aatccggcat acaattgctt tgatcgtccg ggtaaaaaat gtatctcttt
    32221 tacgctacgt atcaacggca aagatcacga tttgttagcg gtagataaaa ttcatgtcga
    32281 tttaaactgg cggtatctga acgagtatct tgatcaaatc agcgctaatg atgaagttct
    32341 atttttgaaa caaggtcatg agatcattgc caagaatcaa ctcgctcgtg aaaaactgat
    32401 tatttataat agcgaaggta attataatat tattgattct gtcgatacgg agtatattgc
    32461 aaagacatca gcagtgccaa ataatgcatt gttcgaaatc tatttttatt atcctggcgg
    32521 taatttattg aacgcatcag ataaactttt ttatctaccg tttgcgttca ttattatcgt
    32581 gttgctggtg gtatatttca tgaccactcg tgtgttccgt cggcaatttt ctgaaatgac
    32641 cgagctggtt aatacgctgg cgtttttgcc cgactcaacg gatcagatcg aggctctgaa
    32701 aattcgcgaa ggcgatgcga aagagattat cagcatcaaa aattcgatcg cggaaatgaa
    32761 agatgccgaa attgaacggt caaataaatt gctctcactg atctcttacg atcaggaaag
    32821 cggttttatt aaaaatatgg cgattattga gtccaacaat aatcagtatc tggctgtggg
    32881 gatcatcaaa ctgtgtggtc tggaagccgt ggaggcggtg tttggtgttg atgaacgcaa
    32941 taaaatcgtc aggaaattgt gtcagcgaat tgccgagaaa tatgcgcaat gctgcgatat
    33001 cgtgacattc aatgccgatc tctatttact tctgtgtcgg gaaaatgtac agacatttac
    33061 ccgtaaaata gcgatggtaa acgattttga cagcagcttt ggctaccgca atctgcgcat
    33121 ccataagtct gccatttgtg aacctttgca gggggaaaac gcctggagtt acgcagaaaa
    33181 actgaaactg gcgatttcca gtatccgtga ccatatgttc tccgagttta ttttctgtga
    33241 tgacgcgaaa ctcaacgaaa tagaagagaa tatctggatt gcgcgtaata ttcgccatgc
    33301 aatggaaatt ggcgaactat tcctcgtcta tcaaccgatc gttgatatta acacccgcgc
    33361 cattctgagc gcggaggcgt tgtgccgttg ggtttctgcg gagcggggga tcatttcacc
    33421 gctgaagttc attaccattg ctgaagatat cgggtttatc aatgagctgg gttatcagat
    33481 tattaaaacg gcaatgggtt aattcagaca ttttagtcaa cgtgcgtcgc tgaaggatga
    33541 tttcttactg catattaatg tttcgccctg gcagttaaac gaaccgcact ttcatgagcg
    33601 ttttaccacc atcatgaaaa aaaatggcct gaaggcgaac agcctctgtg ttgagatcac
    33661 tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac aactgcgtaa
    33721 acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt
    33781 ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg atattttcgg
    33841 tactgcggga aaaattgtgc gcattatttt cgatctggca cgctataacc ggatcccggt
    33901 gattgcggaa ggcgtagaga gcgaagacgt tgcgcgcgaa ttaattaaat taggatgtgt
    33961 tcaggctcag gggtatctgt accagaaacc catgccattc tccgcttggg ataaaagtgg
    34021 aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta cttacagtca
    34081 gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact caaatggtaa
    34141 aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg gtggcaaata
    34201 ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat cccacggagg
    34261 aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc tcttatttgt
    34321 acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag gagttcgaga
    34381 aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa aatacagggt
    34441 ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact cacaatcgta
    34501 gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg ctggtgatgg
    34561 acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc ggtgatgaat
    34621 ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc gcctatttgc
    34681 cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtt tgtacattga
    34741 ttattttctt tgccgcattg gtggcagtga tattgggtgc cagttgcctc tatctggtac
    34801 gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac cattttgaac
    34861 gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt
    34921 ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc tttgatgaag
    34981 atttaaaagc gctgccggaa aaaggcggtt atttgtgtct gtttgacgtc gataaattca
    35041 aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg aaagtggtga
    35101 aaatccttaa atcacagatc ccggtagata aaggtaaagt ctaccgcttc ggaggtgacg
    35161 aatttgcggt gatttatact ggcggcacgc tggaagagtt gctatcgata ctaaaagaaa
    35221 tcgttcattt ccaggtggga agcattaatt taagcaccag tatcggtgta gcgcattcaa
    35281 atgaatgtac taccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg tataagagta
    35341 agaaaaacgg cagggcacag attagctggc agtaatcatt attcgcaggc cggacaaatg
    35401 attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact catcggcgtg
    35461 ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat tgagataaac
    35521 cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt cttcgtccac
    35581 ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct cggcggaaat
    35641 ctgctgctgc agctgcatga aacggttcca gcgttcttct ttcacttctt ccggaacctg
    35701 gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc agccaacgcg
    35761 atccagacgt gcttctttca ggaagtcgag tagcatctgg aaatcttctt ccgtctcgcc
    35821 agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt cgcgccactg
    35881 tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt tgagaatgcg
    35941 cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt ctgccatcag
    36001 tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg tccagatccc
    36061 cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct cgccgttgtg
    36121 gaagccagta cgatgtttaa catcaacacc ataggcggaa gtatcctgcg agatcaccag
    36181 aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt cgccaatcgg
    36241 acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc ggtgattaca
    36301 gccttccgaa attttcagat aggcataatg acgcggcgtc agtttcacac cttgttctgg
    36361 caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag
    36421 aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga cttcgcggat
    36481 ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt cattcaacgc
    36541 ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc aggtgttgac
    36601 gatcaccatg ttcgcatcgt catagctcgg taccacgtca taaccttcag tgcggagttc
    36661 ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa caaagccgat
    36721 tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca
    36781 gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt cgctggcgga
    36841 ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg acagcatttt
    36901 gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag gatcgaagta
    36961 tgttcgttga cagacagcga atcgagctgc tgaaccggtt gatcgacgca cgcgttgacc
    37021 tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc
    37081 atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg ctgcggttga
    37141 atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa gcattagcga
    37201 cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt accgtcaacg
    37261 ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc ctgcagaagc
    37321 acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt
    37381 aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa gccagagtca
    37441 atccggaggc gttatgcatc gacaatcctt tttccttgtg ccccttattt gtctttcttc
    37501 cgctctctgg gcggctcctg caacggtaaa tgtcgaagta ctgcaagaca aactcgacca
    37561 tccctgggca ctggcctttt tacccgataa tcacggtatg ttaatcactc tgcgcggcgg
    37621 cgagttgcgt cactggcaag caggaaaagg attatctgcg ccgctttccg gagttccgga
    37681 cgtttgggcg cacgggcagg gcggcctgct ggacgtggtt ttagcgcctg attttgctca
    37741 gtctcgccgc atctggttaa gttattccga agttggcgat gatggcaaag ccggaactgc
    37801 tgtgggttat ggccgcttaa gtgatgatct ctcaaaagtg accgacttcc gcaccgtctt
    37861 ccgccagatg ccaaaactgt ctaccggcaa ccattttggc gggcggctgg tattcgacgg
    37921 taaaggttat ctttttattg ctctgggcga aaacaatcag cgcccgacgg cgcaggatct
    37981 ggataaatta cagggcaaac tggtgcgtct gaccgaccag ggcgaaatcc cggatgataa
    38041 tccttttata aaggaatccg gtgcgcgcgc cgagatctgg tcttatggca ttcgtaatcc
    38101 gcaaggaatg gcgatgaatc cgtggagtaa tgcactgtgg ctgaatgaac atggcccgcg
    38161 cggtggtgat gaaattaata tcccgcaaaa aggcaaaaac tacggctggc cgctggcaac
    38221 ctggggaatc aactattcag gctttaagat accggaagcg aaaggggaga tcgtcgccgg
    38281 gaccgagcaa cctgtttttt actggaaaga ttcgcccgct gtgagcggca tggccttcta
    38341 taacagcgat aaattccccc agtggcagca aaaattattt attgggagct gaaagataaa
    38401 gatgtcattg tgatgagcgt caacggcgac aaagtgacag aagatggccg tattttaacg
    38461 gacagagggc agcgaattcg tgatgttcgc actggacccg acggttattt atacgttctc
    38521 accgacgagt ccagtgggga attacttaaa gttagcccac gcaattagct aacgggaatc
    38581 atcaccactt tgcgcactgc ggggcgttca gtgagttgct gataccagcg ttgcagattt
    38641 ggacgcggtg tccaggtcag gccaacgtta aacaaattgt agatgaacgg tgcgatagcg
    38701 atatcgccca caccaaactc gtcgccagag aaccatttta cttttgccag ttccgcatcg
    38761 agcagggcaa acagggcgtc gcactctttg caactggcat caatggcggc ctgatcgcgc
    38821 tcttccggtg gtgttctgac taatcccatc aggatcccgc gatgagcatt actgagcgtc
    38881 tggtttgccc agtccatcca tttttccgct tccgcacgac gtgccggtga gtcgatccac
    38941 aggcgttttt gcccgtactg tgccgccaga tagcggacaa tggcgtttga ttcccaaaga
    39001 atgagatcac tttcgtcgtc acgcaacaac ggcaccagcc cgttagggtt catcgccaga
    39061 aaatcagcat cgtgatttat cccaaactca cggcccgcga gaatttgctc ataaggtagt
    39121 tccagttctt cgagcgtcag caatactttt tttacgttag ttgaattatt ccgaccccac
    39181 agcgtaatca tactcacccc ttttccgaca ggcagccaca gactgaacag gactccgatg
    39241 gtgaagtaaa gttaactttt aagcaacagc tggcaaaaaa atgacaccag aagcagcgaa
    39301 gcggcaggat attgcataaa ctttaaaaac tttaccaact tacggtttct ttaagtttgt
    39361 gtgtgcgtta ttaatcaccg aacttatcat acggcgatat aacgtatttt tttgaatgga
    39421 tactcgggtg gcatttatga cgcaatactc ctctctcctt cgtggtcttg cagcgggttc
    39481 tgcattttta ttcctttttg ccccaacggc attcgcggcc gaacaaaccg ttgaagcgcc
    39541 gagcgtggat gcgcgtgcat ggattttaat ggattacgcc agcggtaaag tgctggcaga
    39601 aggcaatgcg gatgagaaac tggatcctgc gagcctgact aaaatcatga ccagctatgt
    39661 ggttgggcag gcgcttaagg ccgataagat taaactcacc gatatggtga cggtcggtaa
    39721 agatgcctgg gcgacgggaa atccggcact gcgtggttca tcggtaatgt tcctcaaacc
    39781 gggagatcag gtttcggtgg cagacttgaa caaaggtgtg attatccagt ccggtaatga
    39841 cgcctgtatt gcgctggcag attacgttgc cgggagccag gagtccttta ttggtctgat
    39901 gaatggttat gccaaaaaac tggggctgac caacactacc ttccagacgg tgcacggcct
    39961 ggatgcgccg gggcagttca gtaccgcgcg cgatatggca ttgctgggta aagcattgat
    40021 ccacgatgtg ccggaagagt acgccattca taaagagaaa gagttcacct tcaacaaaat
    40081 tcgtcagcct aaccgtaacc gtctgctgtg gagcagcaat ctgaatgttg atggcatgaa
    40141 gacaggaacc acggcaggtg cgggatataa tctggttgct tcggctaccc agggtgatat
    40201 gcgtttaatc tccgtagtgc tgggggcgaa aactgaccgt atccgtttta atgagtctga
    40261 gaaattattg acctggggtt tccgcttctt tgaaactgtg acgccaatta aacctgatgc
    40321 cacctttgtg actcagcgcg tctggtttgg tgataagatc gaagtgaatc tcggggcagg
    40381 cgaagcgggc t
//
LOCUS       DAJTOW010000025        39701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTOW010000025 DAJTOW010000000
VERSION     DAJTOW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.025"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..145
                     /locus_tag="OO244_001440"
     CDS             <1..145
                     /locus_tag="OO244_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5663320.1"
                     /translation="MLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQ
                     ELN"
     gene            374..964
                     /gene="uidR"
                     /locus_tag="OO244_001441"
     CDS             374..964
                     /gene="uidR"
                     /locus_tag="OO244_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5663321.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1352..3055
                     /gene="uidA"
                     /locus_tag="OO244_001442"
     CDS             1352..3055
                     /gene="uidA"
                     /locus_tag="OO244_001442"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5663322.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3061..4086
                     /gene="uidB"
                     /locus_tag="OO244_001443"
                     /pseudo
     CDS             3061..4086
                     /gene="uidB"
                     /locus_tag="OO244_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4125..5390
                     /gene="uidC"
                     /locus_tag="OO244_001444"
     CDS             4125..5390
                     /gene="uidC"
                     /locus_tag="OO244_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5663323.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMSFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5616..7124)
                     /locus_tag="OO244_001445"
     CDS             complement(5616..7124)
                     /locus_tag="OO244_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5663324.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNNGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7225..8400)
                     /gene="manA"
                     /locus_tag="OO244_001446"
     CDS             complement(7225..8400)
                     /gene="manA"
                     /locus_tag="OO244_001446"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5663325.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8599..10245
                     /gene="fumB"
                     /locus_tag="OO244_001447"
     CDS             8599..10245
                     /gene="fumB"
                     /locus_tag="OO244_001447"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5663326.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10388..11791
                     /gene="fumC"
                     /locus_tag="OO244_001448"
     CDS             10388..11791
                     /gene="fumC"
                     /locus_tag="OO244_001448"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5663327.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11788..12717)
                     /gene="tus"
                     /locus_tag="OO244_001449"
     CDS             complement(11788..12717)
                     /gene="tus"
                     /locus_tag="OO244_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5663328.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12793..14094)
                     /gene="rstB"
                     /locus_tag="OO244_001450"
     CDS             complement(12793..14094)
                     /gene="rstB"
                     /locus_tag="OO244_001450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5663329.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14273..15592)
                     /locus_tag="OO244_001451"
     CDS             complement(14273..15592)
                     /locus_tag="OO244_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5663330.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15817..16560)
                     /gene="rstA"
                     /locus_tag="OO244_001452"
     CDS             complement(15817..16560)
                     /gene="rstA"
                     /locus_tag="OO244_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5663331.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16689..17024
                     /locus_tag="OO244_001453"
     CDS             16689..17024
                     /locus_tag="OO244_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5663332.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17021..17743)
                     /gene="folM"
                     /locus_tag="OO244_001454"
     CDS             complement(17021..17743)
                     /gene="folM"
                     /locus_tag="OO244_001454"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5663333.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17780..19162)
                     /locus_tag="OO244_001455"
     CDS             complement(17780..19162)
                     /locus_tag="OO244_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5663334.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19348..20292)
                     /locus_tag="OO244_001456"
     CDS             complement(19348..20292)
                     /locus_tag="OO244_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5663335.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20816..22348
                     /gene="pntA"
                     /locus_tag="OO244_001457"
     CDS             20816..22348
                     /gene="pntA"
                     /locus_tag="OO244_001457"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5663336.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22359..23747
                     /gene="pntB"
                     /locus_tag="OO244_001458"
     CDS             22359..23747
                     /gene="pntB"
                     /locus_tag="OO244_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5663337.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23772..24806)
                     /gene="tqsA"
                     /locus_tag="OO244_001459"
     CDS             complement(23772..24806)
                     /gene="tqsA"
                     /locus_tag="OO244_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5663338.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25217..25582
                     /gene="mdtJ"
                     /locus_tag="OO244_001460"
     CDS             25217..25582
                     /gene="mdtJ"
                     /locus_tag="OO244_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5663339.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25569..25898
                     /gene="mdtI"
                     /locus_tag="OO244_001461"
     CDS             25569..25898
                     /gene="mdtI"
                     /locus_tag="OO244_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5663340.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25937..26758)
                     /locus_tag="OO244_001462"
     CDS             complement(25937..26758)
                     /locus_tag="OO244_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5663341.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26858..26941)
                     /locus_tag="OO244_001463"
     CDS             complement(26858..26941)
                     /locus_tag="OO244_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663342.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27034..27369)
                     /locus_tag="OO244_001464"
     CDS             complement(27034..27369)
                     /locus_tag="OO244_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5663343.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27766..29019)
                     /locus_tag="OO244_001465"
                     /pseudo
     CDS             complement(27766..29019)
                     /locus_tag="OO244_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29126..30037
                     /locus_tag="OO244_001466"
     CDS             29126..30037
                     /locus_tag="OO244_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5663344.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30172..31392
                     /gene="mlc"
                     /locus_tag="OO244_001467"
                     /gene_synonym="dgsA"
     CDS             30172..31392
                     /gene="mlc"
                     /locus_tag="OO244_001467"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5663345.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31517..32212
                     /gene="bioD"
                     /locus_tag="OO244_001468"
     CDS             31517..32212
                     /gene="bioD"
                     /locus_tag="OO244_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5663346.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32165..33457)
                     /gene="clcB"
                     /locus_tag="OO244_001469"
     CDS             complement(32165..33457)
                     /gene="clcB"
                     /locus_tag="OO244_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5663347.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33616..34230)
                     /gene="dmsD"
                     /locus_tag="OO244_001470"
     CDS             complement(33616..34230)
                     /gene="dmsD"
                     /locus_tag="OO244_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5663348.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34273..35127)
                     /locus_tag="OO244_001471"
     CDS             complement(34273..35127)
                     /locus_tag="OO244_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5663349.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35129..35746)
                     /gene="dmsB"
                     /locus_tag="OO244_001472"
     CDS             complement(35129..35746)
                     /gene="dmsB"
                     /locus_tag="OO244_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5663350.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35757..37796)
                     /locus_tag="OO244_001473"
                     /pseudo
     CDS             complement(35757..37796)
                     /locus_tag="OO244_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37857..38966)
                     /locus_tag="OO244_001474"
     CDS             complement(37857..38966)
                     /locus_tag="OO244_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5663351.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38949..>39701
                     /locus_tag="OO244_001475"
     CDS             38949..>39701
                     /locus_tag="OO244_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5663352.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSS"
BASE COUNT        10093 a         9628 c        10159 g         9821 t
ORIGIN      
        1 aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg ctaacgcggc
       61 gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca ccgtctccgg
      121 tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc gtatgtgttg
      181 tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg cagccaaatc
      241 acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt accttcgcac
      301 tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta ttcacgctaa
      361 ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac ggacccggat
      421 cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct cgatgaaagc
      481 catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca tctccaaaga
      541 agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc gtttccggga
      601 accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct ctctcaccca
      661 tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga tgcgtaaccc
      721 acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg ttgcccagcg
      781 gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg caatgacttc
      841 acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg aagacctggc
      901 gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat taaccgcatc
      961 ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat cgtaatttct
     1021 ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat tcaccgtagc
     1081 catatgtcat gagagtttat cgttcccaat actctcgaac gaacgttcga ttgcttattt
     1141 tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc tgtgggtatt
     1201 gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc tcttccatgg
     1261 gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat taaaggttga
     1321 ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa ccccaacccg
     1381 tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
     1441 tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag tgccaggcag
     1501 ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg tctggtatca
     1561 gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
     1621 ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
     1681 ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
     1741 caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
     1801 cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccgggat
     1861 ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
     1921 gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
     1981 tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
     2041 cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
     2101 tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc tgcgcgtcgg
     2161 catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt
     2221 tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg ataacgtgct
     2281 gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc gtacctcgca
     2341 ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
     2401 tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
     2461 gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg cgcacttaca
     2521 ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
     2581 tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga
     2641 agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga
     2701 cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg
     2761 ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaactgct
     2821 ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt
     2881 agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct
     2941 ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa
     3001 tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca attgacctac
     3061 atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact tgctaccgcg
     3121 atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat tgccgcttca
     3181 ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc cagcccggaa
     3241 gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg aatggtgctt
     3301 tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc gcagccgtca
     3361 ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt gtgcatcggt
     3421 gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt ctacgtgcgc
     3481 tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa cctggttggt
     3541 actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa aaagaatacc
     3601 ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg ggtttccgtc
     3661 tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca gggcgttact
     3721 atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata cctgaccggc
     3781 gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg cggtcaggca
     3841 atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc caatcaggtg
     3901 taaacgtcgg aagttattat gggcatccgc acatcaattg ccttagtacc ttgcggattt
     3961 atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa attcaaagaa
     4021 atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag cgatatcact
     4081 aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga aaaatagtgg
     4141 ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac gcggcgcaac
     4201 tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc agggctgata
     4261 agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga ttgctcgatt
     4321 tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat tatgtttata
     4381 aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat aaaagttttg
     4441 gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt aaattaggtc
     4501 gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta atggttggca
     4561 gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg gctttaacgc
     4621 caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact gattcaacaa
     4681 ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat aaacatcgcg
     4741 cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct ctgggggcat
     4801 cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc ataccgctcg
     4861 acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc gaaggtttaa
     4921 gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg acctggaatg
     4981 cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg aatggtgccg
     5041 tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac cgtgaaggaa
     5101 tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg ctgacatttg
     5161 caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt gaaggcgcgg
     5221 gtatcttagg tggtatgaac tatcgtgtca gcgaagggcc gttacaaggg atgagtttct
     5281 ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc gatcgcctga
     5341 attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa gaaataacgc
     5401 cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt ttatgccgga
     5461 tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg ttgcaggaaa
     5521 aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca gatgaaaacg
     5581 ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat tgccggtaca
     5641 gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa cgacattttc
     5701 tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat ggtattgtcc
     5761 tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac ttgctgtttc
     5821 gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg agtcatcaac
     5881 tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac cgaacgatct
     5941 acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt caggaacagc
     6001 gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc gatagtaatc
     6061 accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt cactttctcc
     6121 tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg agtttgcgcg
     6181 ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac ttttaaagtc
     6241 agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga gtaatccagt
     6301 tggctattga tcgttttacc gttattgacc agatccgatt taccgctgat ctccatgcct
     6361 tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag tgacagtttt
     6421 tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt tttcagatta
     6481 ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg accactttgc
     6541 gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg gaactcgccg
     6601 ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag cgaaatatcg
     6661 gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt ttcacctttt
     6721 gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg aattgatgcc
     6781 atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc atgatcaacc
     6841 gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt cccggcaatg
     6901 ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt ttgataactc
     6961 acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg cgcgaccatg
     7021 tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt ccagactacg
     7081 cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat tatccataaa
     7141 atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag cttagcaaga
     7201 gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta aacggccgtg
     7261 gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac ccggtttaag
     7321 ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga acaaaatggc
     7381 ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg agaaggcaaa
     7441 atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct gggtcaacaa
     7501 ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa tatcaatgta
     7561 tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt ccagcgccac
     7621 gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc caggattcaa
     7681 tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt aaaattcaga
     7741 aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg attttaaaat
     7801 cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga acagttcgct
     7861 taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg caccggcgac
     7921 cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg caaggaaagg
     7981 cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac gctcggcggc
     8041 atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat tgtgtttgtt
     8101 tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca ggaaaggcag
     8161 ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac tctcaatcac
     8221 atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc ttttcggatg
     8281 tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac cataaagttc
     8341 agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga gtttttgcat
     8401 gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa tcctacggga
     8461 agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa actgtgtagg
     8521 atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata accaaaccag
     8581 gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc ttttccactc
     8641 aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt atctgaattt
     8701 gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc gcgtcaggcg
     8761 tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc cgacattctg
     8821 cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct gcgtaactcc
     8881 gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc gattattgtt
     8941 ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct ggcgcgcggt
     9001 gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc gctggatatg
     9061 tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta tgccgttgat
     9121 ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa caagacgtat
     9181 ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta cctggttgag
     9241 aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt cgttattggt
     9301 ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa atactatgat
     9361 gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga actggaaaaa
     9421 gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg taaatacttc
     9481 gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc ggttggtatg
     9541 ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca ggggatctgg
     9601 atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg taaagcggga
     9661 gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct cgcacagttg
     9721 tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt tggtcgtgat
     9781 attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc gcaatacatt
     9841 aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta tgcctccggt
     9901 tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact gcaagcgcag
     9961 ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac ggatgcctgt
    10021 aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt attggcgcag
    10081 ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga agccatctgg
    10141 aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg aaatgacttc
    10201 ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga gccgcccttc
    10261 ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata gtaaataatg
    10321 agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat caggtgagga
    10381 gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg atgtcccggc
    10441 agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca tttcgacgga
    10501 gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag cggcaaaagt
    10561 taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc aggcggcgga
    10621 tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc agaccggctc
    10681 cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca gtgaattact
    10741 cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga acaaaagcca
    10801 aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg cactgcgcaa
    10861 gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat cgcgtgcatt
    10921 tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc taacactagg
    10981 gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata tcgaatacag
    11041 cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg gactaaatac
    11101 ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct gtgcaccgtt
    11161 tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg ttcaggcgca
    11221 cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg tccgctggct
    11281 ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg agccgggcag
    11341 ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca tgctctgctg
    11401 tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta actttgaact
    11461 gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct tgctggcaga
    11521 tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc gtgagcgaat
    11581 caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc acattggtta
    11641 tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct taaaagctgc
    11701 ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac ggccagaaca
    11761 gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca gccgtggaat
    11821 gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt cggcatcgta
    11881 atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa tcagtgcaat
    11941 cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc agacgcgggc
    12001 aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg gcagggcagc
    12061 gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc acggtgcgac
    12121 actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac gatgcaaatt
    12181 cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt gcagaacggt
    12241 gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca cccattcaaa
    12301 acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg tggtcttgag
    12361 tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat cgacctggta
    12421 acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg actgttgttg
    12481 aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc cgagatgttg
    12541 ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct ccggcaaaga
    12601 gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg tagccagctc
    12661 ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac gcgccataat
    12721 attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga cttgaccatc
    12781 gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg gccagctaaa
    12841 cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc ccattgtcag
    12901 tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc gatcccggct
    12961 gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg caatccctgg
    13021 gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc tggtttcaac
    13081 cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc gctccattaa
    13141 gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta ccgttttatc
    13201 gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca ggtctggttc
    13261 gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca gctcttcaat
    13321 taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg cgctcaggtt
    13381 atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt cgtgagcgat
    13441 accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg ccatctggtt
    13501 aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac gttcattgag
    13561 atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct gccagtgcgg
    13621 acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga tcagggcgat
    13681 atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa ctgccagcac
    13741 gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg cgacaatttc
    13801 gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca gtggctcgac
    13861 acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc agtcgtgtgg
    13921 ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca aatcatccag
    13981 cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca gcagagacat
    14041 cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt tcattggcat
    14101 cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt aaaccgccaa
    14161 ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc cccggtgtat
    14221 tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag gtttaattta
    14281 acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct gatattacac
    14341 cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg atcggctcta
    14401 tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg acaatcagcg
    14461 ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc gcgatggcag
    14521 gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt ggagcaatca
    14581 tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg ctggaaccaa
    14641 aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat gcagaaatag
    14701 ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt acttgccaga
    14761 cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc gagtgaacaa
    14821 taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa ttggtaaact
    14881 gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca taaggcgtca
    14941 gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg atccccgcgc
    15001 tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca ataccagcaa
    15061 gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc agatcaagga
    15121 agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca gctgttttcg
    15181 agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca tcaaggccaa
    15241 ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac aacatacagg
    15301 caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta ttaatcgctg
    15361 aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta acataaccgt
    15421 taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga atgaaatgca
    15481 gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg gaaagggatt
    15541 cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc acaatatatg
    15601 tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa tgccggaaag
    15661 agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat agagaaattt
    15721 tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct tattaattga
    15781 attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt gtttattgtc
    15841 actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt taatgcgata
    15901 aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca cgtccacgct
    15961 acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat cgcggtccat
    16021 gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg tcgagagcga
    16081 gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta acgtgccgaa
    16141 atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca gtgtggcttg
    16201 ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg gcgtcgtttt
    16261 gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat cgctatcgag
    16321 agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac aaatggtcat
    16381 gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt ctcgcaaaat
    16441 ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat gttttgccag
    16501 gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga tagtgttcat
    16561 aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac ttactatcaa
    16621 tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg aagtgttaag
    16681 gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg ctgttgattg
    16741 gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt ttcccgacct
    16801 ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc ttacgcgcaa
    16861 ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg tcgctgtggt
    16921 atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg tgttggggga
    16981 taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga tgacgaccgc
    17041 catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa tcgaccaggt
    17101 cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc gcctgttgtc
    17161 gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata gaattcactt
    17221 tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt gccgctttgc
    17281 ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg gtaaagtgaa
    17341 tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca tggttgagca
    17401 ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc agtggcgcac
    17461 ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga ccatgggtgg
    17521 tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa tcagcctgaa
    17581 tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc cgatagctga
    17641 caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg atgcgacgac
    17701 ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc tcctttgcta
    17761 tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact gaggcaatta
    17821 acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt gtatgcgttt
    17881 tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat aacagcaagt
    17941 gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg acacctaccg
    18001 ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg aaatagggca
    18061 ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg gtcagccaga
    18121 tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg gatggcgcag
    18181 cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca gccaggaacg
    18241 gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa acgatcaaac
    18301 cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga ccggccattg
    18361 acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa agcagcgtta
    18421 ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg ccaacatcac
    18481 gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg aaaacccaca
    18541 gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt gcaagtccgg
    18601 tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca acaaacagac
    18661 ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg gtttgcaccc
    18721 cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact atcgattgcc
    18781 aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag cttaacgcgg
    18841 aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag tatccccatg
    18901 cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag ataccgccat
    18961 caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat aaaatgccag
    19021 cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc gccatatttt
    19081 gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg gtgagtgcgc
    19141 tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag aagaataaat
    19201 attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt tacgaagaga
    19261 tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa ggcggcatca
    19321 agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga cggtcaggtt
    19381 attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt tcttcgcggc
    19441 acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag agaatttgat
    19501 gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga ctttagtgcc
    19561 atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa agaagcgacc
    19621 gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt tcttcgcggc
    19681 ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga tcacatccgg
    19741 gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa tacgctggtt
    19801 gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac ctttcgcttt
    19861 tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc cctggacggt
    19921 gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga caacaccgtt
    19981 aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga ggtcagcgac
    20041 cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa aagaggcggc
    20101 accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag cattaaaacg
    20161 accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg gtgtcagttc
    20221 tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca ggagggtgtt
    20281 cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt attgttatta
    20341 acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc atttttcata
    20401 tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt aaaaaacagc
    20461 cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta atgttaagta
    20521 gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat tatcagtctt
    20581 tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat attttgcgag
    20641 ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca gtacgttata
    20701 gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat gagcagcttg
    20761 tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga atatcatgcg
    20821 aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa cgccaaaaac
    20881 agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg cgggtcaact
    20941 ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag aagggaatag
    21001 cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg aaattgcgtt
    21061 actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc cggaattaat
    21121 gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc cgcgtatctc
    21181 acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt atcgcgccat
    21241 tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg cggccgggaa
    21301 agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg ccgccattgg
    21361 cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg aagtgaaaga
    21421 acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg aagcgggcag
    21481 cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa tggaactctt
    21541 tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc caggcaaacc
    21601 agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca gtgtgattgt
    21661 cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg aaatcttcag
    21721 tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc tgccgacgca
    21781 atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt gcaaagagaa
    21841 agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga ccgtgatccg
    21901 tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc cgcaggcggc
    21961 acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac cgtggcgtaa
    22021 atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg ttgcgccgaa
    22081 agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt attacgtggt
    22141 gtggaatgta tcgcacgcgc tgcatacacc gctgatgtcg gttaccaacg cgatttcagg
    22201 gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta gcttccttag
    22261 ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg tgactcagcg
    22321 catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga ttagttacag
    22381 ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt tcgaaacatg
    22441 aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg ttaatcgcaa
    22501 ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg gtcattggtg
    22561 gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca gaactggtgg
    22621 cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac agttatctgc
    22681 atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa gtgttcctcg
    22741 gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc aaactgtgtg
    22801 gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac ctggcggctc
    22861 tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc ggcctgcaag
    22921 tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat ttagtcgcct
    22981 ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac tccggctggg
    23041 cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc ggtgcgctgg
    23101 tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac cgttccttta
    23161 tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat gatcaggaag
    23221 tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa aactcccatt
    23281 cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat cctgtcgctg
    23341 aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc cacccggttg
    23401 cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta ccgtatgaca
    23461 tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc gtactggtga
    23521 ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt ccgattgctg
    23581 gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa cgttcgatga
    23641 acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc cacatgctgt
    23701 ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc tgacggcctc
    23761 tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct ttgacctccc
    23821 gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag cagcataccc
    23881 accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa tgtcgaaagc
    23941 cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag attaatcagc
    24001 agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag tacctgagcg
    24061 atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag cgcaaaggcc
    24121 agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc ccaggcgacc
    24181 aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac actgtcaatc
    24241 gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg aaattttccg
    24301 ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa aaatattgat
    24361 gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc cgcgttcgga
    24421 tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg caacaacggc
    24481 tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt ccgcgtcaac
    24541 tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac gatgattgtc
    24601 atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg ttgcaccagc
    24661 gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat ctcggcggca
    24721 aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat ttttaggcca
    24781 ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt gctaataata
    24841 atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa gaagtgaaaa
    24901 tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta ccgaggacaa
    24961 ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat ggacaatttg
    25021 ttcaggacgt tattttctac atttacccat cttcgtacct catccactat cttgctcgta
    25081 ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct tgccctatgc
    25141 gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa aatagttctc
    25201 ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta ttgctacaga
    25261 aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg gcggctttat
    25321 tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg ttaaaaaaat
    25381 cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta ttaccttgtt
    25441 tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt taaccaccct
    25501 ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac ctgaactgga
    25561 ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg gcaatcgtgc
    25621 tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc aaaatatttg
    25681 gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag gccgttaaag
    25741 ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc gccacgttag
    25801 ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt ggcctggtct
    25861 tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc ccgcgctgtc
    25921 gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga aaaccggtaa
    25981 cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc gaactttgca
    26041 ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg ctgtcacccg
    26101 gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc acttcacagt
    26161 tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga gtcactttac
    26221 gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat aacggcaacg
    26281 gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac ggtgcagctg
    26341 cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt gtcggatcaa
    26401 cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac acaaaacgca
    26461 gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt cctgccgtta
    26521 atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc gtttccagtt
    26581 gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga tcatcatgac
    26641 caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt ttatctgcaa
    26701 acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt gtacgcatat
    26761 cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac tatagacggg
    26821 acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa aaacgggcgg
    26881 caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa cggcccacca
    26941 tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta attttcaggc
    27001 gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg cagcaggttt
    27061 tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg ctttttgttc
    27121 aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct gcgctttctg
    27181 ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg ctgctttggt
    27241 ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg cagaagacag
    27301 acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac cctcaatttg
    27361 ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg gaatggcaga
    27421 cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa taaattacag
    27481 cgttgataat aacgtattgt gacttaagga aaatttagct accaataata gtagtcttga
    27541 tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca gtaataaatg
    27601 acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac gctagatgcg
    27661 ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt ggtttgagca
    27721 ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg cgtgcagacg
    27781 acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa taaatgcgcc
    27841 gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg caatactcga
    27901 ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc gttttgcgcg
    27961 ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg agaagagtaa
    28021 cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac caaacggcat
    28081 aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca tggttccggc
    28141 tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa ccacggcctg
    28201 actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca gcgtgacgaa
    28261 cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt cacgccagtg
    28321 cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa aatggcgtga
    28381 ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac cgattgccgc
    28441 cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc gtccgctcat
    28501 gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga aactggggtg
    28561 gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg aaagcccaat
    28621 caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa gtaacgtaca
    28681 aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg catcggatag
    28741 cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca gtgaaatact
    28801 actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag gctgcacaca
    28861 atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga cgcgcataaa
    28921 ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct tgtcagtgtc
    28981 gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt gctaaatatg
    29041 cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct aatatattaa
    29101 taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc gttactttgt
    29161 tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata tttcgcaacc
    29221 gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac tgctggcacg
    29281 aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca
    29341 aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc
    29401 gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac
    29461 gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg aaatgaacac
    29521 tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac
    29581 agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat
    29641 cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac tggctgaact
    29701 ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga ctgggctgta
    29761 tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca ctcaggaggt
    29821 gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt caattttgcc
    29881 tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg ctgaagagga
    29941 tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc cagctgcgcg
    30001 taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc gaaaaagccc
    30061 gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta ttgaagtgct
    30121 tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc ggtggttgct
    30181 gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc ggtttatcgc
    30241 ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc gcagctggct
    30301 cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt gcgagagctg
    30361 gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt tgaaactgaa
    30421 gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc tctgcgcgat
    30481 ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga tgacttgcca
    30541 ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca gaaaaaactt
    30601 gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga aaatggtatt
    30661 gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga ggcgctggag
    30721 cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac gatggcagag
    30781 gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat cgatcacaac
    30841 gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag tagtctcgtg
    30901 gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg gaaccacggc
    30961 tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct gcgtctcaat
    31021 caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt gtgtcaggcg
    31081 gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc gcatgtaggg
    31141 cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat tggctcaccg
    31201 ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg tcagcaggcc
    31261 cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa ccagggcacg
    31321 atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt gattcgtctg
    31381 ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca atttgggcca
    31441 ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt tgtgaagcat
    31501 aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca cttctgtagg
    31561 gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa aaagggttgc
    31621 gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc gtaataaaga
    31681 tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag ttaatcctat
    31741 cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt acaccctcat
    31801 ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag aagggactgg
    31861 cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag tgcaggaaca
    31921 actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg cactgctaac
    31981 agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta accgaatcaa
    32041 cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac ttccggcacc
    32101 gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg ggcaatacat
    32161 ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg
    32221 aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga tactccccgg
    32281 tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc gccaacagtg
    32341 tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg aaccataatc
    32401 ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga
    32461 caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta caaaggaaga
    32521 tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg gtgctatagc
    32581 cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac ccgcctagtg
    32641 ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag gcgttcatta
    32701 acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata atcagcgcat
    32761 agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg tcgctatgat
    32821 taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg ccgagagagg
    32881 ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca agcggggcac
    32941 gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc cattcctggc
    33001 gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga atcatcgcac
    33061 cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc gatttaacca
    33121 ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa tcggtcggcg
    33181 catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc gccgccagtc
    33241 cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct gcattgacca
    33301 gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca tgacgaaacc
    33361 cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga cggcggaaca
    33421 tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg caatgaaaca
    33481 cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc tttaagagat
    33541 aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc accatccggc
    33601 aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca acagttgtga
    33661 ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga aagggtgttc
    33721 tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt gccatgccag
    33781 caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca tcaacagcag
    33841 tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc ctttctcgcg
    33901 catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc gatccagcca
    33961 gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac gctgccaggc
    34021 ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg ctaatgacgc
    34081 ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa ccagaggtgc
    34141 ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg ccgcgacaga
    34201 aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg cgggttgccc
    34261 cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc cataaaagag
    34321 cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa gtccggcaac
    34381 gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg cgcataacaa
    34441 taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg cttgttgcac
    34501 ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg cacaagcgat
    34561 cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc gggctgcccg
    34621 cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga aagccagcgt
    34681 agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg tcatcatcca
    34741 gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca acgcttgcgg
    34801 cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa atatggaacc
    34861 ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt taaacgctcg
    34921 cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca acagccagag
    34981 gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg cgaaccagcc
    35041 aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa agataaccag
    35101 tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc agataaccag
    35161 tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg ggttttgtga
    35221 aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg cgcagctctt
    35281 caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg ggttgtttcc
    35341 cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt tcagcattgt
    35401 actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata caaacatctt
    35461 catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg cagacttttg
    35521 tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg aacacgttct
    35581 ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa atacggcgga
    35641 aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag gttttacagc
    35701 cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc tactccttaa
    35761 accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag cggtgaaggg
    35821 cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc aaacatatcc
    35881 gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg cggagtcact
    35941 tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc gccatgacgg
    36001 ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca ggcctgctgt
    36061 aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg acttcactca
    36121 gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca
    36181 catagcccat attccctgca gattcatgag agataaggtc ttcttgctct gttggcatca
    36241 ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg tcaatgccaa
    36301 caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg atgtcgccat
    36361 gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg acatccagtt
    36421 tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg tcgatggcat
    36481 ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc gggaaccatt
    36541 ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt atgccgacgt
    36601 taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg ttggagtgtc
    36661 gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc tctcgtgcca
    36721 actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc ggcgttttag
    36781 ctatgccgtc aggcccttcg cccagaatat aggcatttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat gctacttaag
    38941 gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt tctttctctg
    39001 ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg ctgacacaaa
    39061 tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt cgatatacta
    39121 tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc cgcagcacag
    39181 acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag gccttcatgt
    39241 tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct atggctgagc
    39301 agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct actctggtcg
    39361 acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg gaccagcaga
    39421 agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc gacactggtg
    39481 atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac tcaactatag
    39541 gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt ttcacacagg
    39601 agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct gagaaggtcc
    39661 agaccctgaa gacatccagc tacaaggacc tctcgtcctc a
//
LOCUS       DAJTOW010000026        38785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTOW010000026 DAJTOW010000000
VERSION     DAJTOW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.026"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO244_001476"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO244_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5663353.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="OO244_001477"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="OO244_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5663354.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="OO244_001478"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="OO244_001478"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5663355.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="OO244_001479"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="OO244_001479"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5663356.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="OO244_001480"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="OO244_001480"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5663357.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="OO244_001481"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="OO244_001481"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5663358.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="OO244_001482"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="OO244_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5663359.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="OO244_001483"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="OO244_001483"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5663360.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="OO244_001484"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="OO244_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5663361.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="OO244_001485"
     CDS             7139..7750
                     /locus_tag="OO244_001485"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5663362.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO244_001486"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO244_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5663363.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO244_001487"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO244_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO244_001488"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO244_001488"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5663364.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="OO244_001489"
     CDS             complement(11944..12303)
                     /locus_tag="OO244_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663365.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILVAIK"
     gene            complement(12300..12806)
                     /locus_tag="OO244_001490"
     CDS             complement(12300..12806)
                     /locus_tag="OO244_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663366.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13515)
                     /locus_tag="OO244_001491"
     CDS             complement(12952..13515)
                     /locus_tag="OO244_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663367.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="OO244_001492"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="OO244_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="OO244_001493"
     CDS             complement(14722..15096)
                     /locus_tag="OO244_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5663368.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO244_001494"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO244_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5663369.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO244_001495"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO244_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5663370.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="OO244_001496"
     CDS             complement(17166..18056)
                     /locus_tag="OO244_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5663371.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OO244_001497"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OO244_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5663372.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OO244_001498"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OO244_001498"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5663373.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OO244_001499"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OO244_001499"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5663374.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OO244_001500"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OO244_001500"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5663375.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OO244_001501"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OO244_001501"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5663376.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OO244_001502"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OO244_001502"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5663377.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OO244_001503"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OO244_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5663378.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OO244_001504"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OO244_001504"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5663379.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OO244_001505"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OO244_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5663380.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKCLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OO244_001506"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OO244_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5663381.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OO244_001507"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OO244_001507"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5663382.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OO244_001508"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OO244_001508"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5663383.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OO244_001509"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OO244_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5663384.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OO244_001510"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OO244_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5663385.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OO244_001511"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OO244_001511"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5663386.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36950)
                     /gene="fcl"
                     /locus_tag="OO244_001512"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35982..36950)
                     /gene="fcl"
                     /locus_tag="OO244_001512"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5663387.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36953..38074)
                     /gene="gmd"
                     /locus_tag="OO244_001513"
     CDS             complement(36953..38074)
                     /gene="gmd"
                     /locus_tag="OO244_001513"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5663388.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38100..38648)
                     /gene="wcaF"
                     /locus_tag="OO244_001514"
     CDS             complement(38100..38648)
                     /gene="wcaF"
                     /locus_tag="OO244_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5663389.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38664..>38785)
                     /locus_tag="OO244_001515"
     CDS             complement(38664..>38785)
                     /locus_tag="OO244_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5663390.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10358 a         9714 c         8726 g         9987 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccaaggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggaccaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtaattacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgaggtatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgacaa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtatctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacaa tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaaaccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 tttgataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcactgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaacac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg tgagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt cctgcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagtac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgatccc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aaccacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcac gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgatggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ccgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacatttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccag gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccgct
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ccctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcggcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 ggcgcattct gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag
    36421 ttactcgggt ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa
    36481 tcgcgtccat actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg
    36541 gcgtaaggct cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc
    36601 tgttttgcca gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg
    36661 ttctgatgcg cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc
    36721 gccggatagg tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg
    36781 tcaatgcgtt cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca
    36841 tcgcgggtac gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca
    36901 gaaccgacca tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact
    36961 ccagcgcgat cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt
    37021 cgaggtcatt agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc
    37081 ccagtttttc gtgcgctttg gtcgggtcac cgagcagcgt ttcaacttct gccggacgga
    37141 agtaacgcgg atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc
    37201 cggtgacgga aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga
    37261 tgcccagctg tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg
    37321 cgataacgaa atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt
    37381 ctttggcgtg gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca
    37441 gcccctgggc gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc
    37501 ggcgcgggga ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc
    37561 ggtagttaac ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt
    37621 agaacggcgt ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg
    37681 aagcctgata gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc
    37741 gcagcgtacc catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa
    37801 cgtggctcat tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg
    37861 tcaggttaga ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt
    37921 gcggatcctg ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa
    37981 taccatgcac ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc
    38041 cggttacacc ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt
    38101 tattcagttt caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt
    38161 gccggaagcg atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg
    38221 acgcctgggg caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc
    38281 gtggcgttaa tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt
    38341 ttttgcgata tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg
    38401 tcatcgccaa cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact
    38461 gacggacgaa taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc
    38521 cgccagcgat acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat
    38581 aattgcactt taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa
    38641 tcttgcattg gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc
    38701 gtacgttggc gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc
    38761 cgttggactt ttttggcgtc agcac
//
LOCUS       DAJTOW010000027        37957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTOW010000027 DAJTOW010000000
VERSION     DAJTOW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.027"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(53..1402)
                     /gene="lysC"
                     /locus_tag="OO244_001516"
                     /pseudo
     CDS             complement(53..1402)
                     /gene="lysC"
                     /locus_tag="OO244_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
     gene            1927..3576
                     /gene="pgi"
                     /locus_tag="OO244_001517"
     CDS             1927..3576
                     /gene="pgi"
                     /locus_tag="OO244_001517"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5663391.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4039..4281
                     /locus_tag="OO244_001518"
     CDS             4039..4281
                     /locus_tag="OO244_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663392.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4395..5033
                     /locus_tag="OO244_001519"
     CDS             4395..5033
                     /locus_tag="OO244_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5663393.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5030..5767
                     /locus_tag="OO244_001520"
     CDS             5030..5767
                     /locus_tag="OO244_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5663394.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5767..7863
                     /locus_tag="OO244_001521"
     CDS             5767..7863
                     /locus_tag="OO244_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5663395.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7910..8188)
                     /locus_tag="OO244_001522"
     CDS             complement(7910..8188)
                     /locus_tag="OO244_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663396.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8189..8332
                     /locus_tag="OO244_001523"
     CDS             8189..8332
                     /locus_tag="OO244_001523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663397.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8458..8868
                     /gene="psiE"
                     /locus_tag="OO244_001524"
     CDS             8458..8868
                     /gene="psiE"
                     /locus_tag="OO244_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5663398.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8912..10387)
                     /gene="xylE"
                     /locus_tag="OO244_001525"
     CDS             complement(8912..10387)
                     /gene="xylE"
                     /locus_tag="OO244_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5663399.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10758..11648)
                     /gene="malG"
                     /locus_tag="OO244_001526"
     CDS             complement(10758..11648)
                     /gene="malG"
                     /locus_tag="OO244_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5663400.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11663..13207)
                     /gene="malF"
                     /locus_tag="OO244_001527"
                     /pseudo
     CDS             complement(11663..13207)
                     /gene="malF"
                     /locus_tag="OO244_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            complement(13361..14551)
                     /gene="malE"
                     /locus_tag="OO244_001528"
     CDS             complement(13361..14551)
                     /gene="malE"
                     /locus_tag="OO244_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5663401.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14916..16031
                     /gene="malK"
                     /locus_tag="OO244_001529"
     CDS             14916..16031
                     /gene="malK"
                     /locus_tag="OO244_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5663402.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16103..17443
                     /gene="lamB"
                     /locus_tag="OO244_001530"
     CDS             16103..17443
                     /gene="lamB"
                     /locus_tag="OO244_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5663403.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17595..18515
                     /gene="malM"
                     /locus_tag="OO244_001531"
     CDS             17595..18515
                     /gene="malM"
                     /locus_tag="OO244_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5663404.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            18744..20324
                     /locus_tag="OO244_001532"
                     /pseudo
     CDS             18744..20324
                     /locus_tag="OO244_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            20547..21044
                     /gene="ubiC"
                     /locus_tag="OO244_001533"
     CDS             20547..21044
                     /gene="ubiC"
                     /locus_tag="OO244_001533"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5663405.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            21057..21929
                     /gene="ubiA"
                     /locus_tag="OO244_001534"
     CDS             21057..21929
                     /gene="ubiA"
                     /locus_tag="OO244_001534"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5663406.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(22084..24507)
                     /gene="plsB"
                     /locus_tag="OO244_001535"
     CDS             complement(22084..24507)
                     /gene="plsB"
                     /locus_tag="OO244_001535"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5663407.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            24678..25046
                     /gene="dgkA"
                     /locus_tag="OO244_001536"
     CDS             24678..25046
                     /gene="dgkA"
                     /locus_tag="OO244_001536"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5663408.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            25156..25764
                     /gene="lexA"
                     /locus_tag="OO244_001537"
     CDS             25156..25764
                     /gene="lexA"
                     /locus_tag="OO244_001537"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5663409.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            25837..27162
                     /gene="dinF"
                     /locus_tag="OO244_001538"
     CDS             25837..27162
                     /gene="dinF"
                     /locus_tag="OO244_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5663410.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            27278..27487
                     /locus_tag="OO244_001539"
     CDS             27278..27487
                     /locus_tag="OO244_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5663411.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(27529..28044)
                     /gene="zur"
                     /locus_tag="OO244_001540"
     CDS             complement(27529..28044)
                     /gene="zur"
                     /locus_tag="OO244_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5663412.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            28362..29347
                     /locus_tag="OO244_001541"
                     /pseudo
     CDS             28362..29347
                     /locus_tag="OO244_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            29710..30747
                     /gene="dusA"
                     /locus_tag="OO244_001542"
     CDS             29710..30747
                     /gene="dusA"
                     /locus_tag="OO244_001542"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5663413.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            30881..31123
                     /gene="pspG"
                     /locus_tag="OO244_001543"
     CDS             30881..31123
                     /gene="pspG"
                     /locus_tag="OO244_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5663414.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(31289..32272)
                     /locus_tag="OO244_001544"
     CDS             complement(31289..32272)
                     /locus_tag="OO244_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5663415.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            32355..33770
                     /gene="dnaB"
                     /locus_tag="OO244_001545"
     CDS             32355..33770
                     /gene="dnaB"
                     /locus_tag="OO244_001545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5663416.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            33823..34902
                     /gene="alr"
                     /locus_tag="OO244_001546"
     CDS             33823..34902
                     /gene="alr"
                     /locus_tag="OO244_001546"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5663417.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            35155..36348
                     /gene="tyrB"
                     /locus_tag="OO244_001547"
     CDS             35155..36348
                     /gene="tyrB"
                     /locus_tag="OO244_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5663418.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(36851..37055)
                     /locus_tag="OO244_001548"
                     /pseudo
     CDS             complement(36851..37055)
                     /locus_tag="OO244_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37457..>37957
                     /gene="aphA"
                     /locus_tag="OO244_001549"
     CDS             37457..>37957
                     /gene="aphA"
                     /locus_tag="OO244_001549"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5663419.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIF"
BASE COUNT         9504 a         9221 c         9704 g         9528 t
ORIGIN      
        1 gtgacaagaa aatcaatacg gcccgaaata tagcttccag gccatacagt atttactcaa
       61 acaaattact atgcagtttt tgcaccacct gctcggcatc ttcgccgggc accaggaagc
      121 acaggttatg gctggatgcg ccgtaacaaa tcatgcgaat gttgaacggt tccagtacgc
      181 cgaatacctc tttgccaacg ccgcaggctt ttgacaggtc attgccaatc aacgcgacca
      241 gcgccagacc ttcttccacc tccacccggc acagtgcgga aagctccatc agcagagatt
      301 acgtcagcaa cgtatcgcca gtggaggttg aaccggtggt atcaagggtt aatgccacgc
      361 tcacttctga cgtggtgatt aagtctaccg aaatattatg ccgcgcgagg atgccgaaaa
      421 cttccgcgag gaagccgcga gaatgcagca tattcaggct gtgcaaagtg agcagagtct
      481 gattgcgacg aagcgccagc gcgcggaaca gcggcggatt ttcagtttta ttgcacacca
      541 gcgtaccacc tgcgcgtggg tctttgctgg agccgacaaa gaccgggata tcgctgcgta
      601 ctgcgggtag caacgttgcc ggatgcagta cttttgcacc aaaagttgcc atctctgccg
      661 cttcggcaaa cgcgatttca tcaatgcgtt ttgctgcgga aactacgcgt ggatcggtgg
      721 tgtagatgcc cgggacgtcg gtccagatat caacacgaga tgcgtgtaaa gcctccgcca
      781 gcaaggctgc cgtataatcg ctgcctccac ggccaagcgt cgttgtacga cctttatttt
      841 cgctaccgat aaatccctgg gtgatcacta agccttcatt gaggcgtggg agcagctgca
      901 gcgcggccag ttccgccagc gcggctacat ctggctctgc acgaccaaat cggtcgttgg
      961 tacgcatcac tttacgtaca tcaaaccact gtgcctgaac atcgcgttcg cgcaggattt
     1021 cgacaaacag cagggtcgac atcagctcgc cgtggctgac cagctcatct gtcagcgccg
     1081 gagacgttgc cagcgccgcc gcttctgcca gaacagtaat gttctccagc agacgttcaa
     1141 tctcttcacg gataacgttc gggtaacgca gacgttccag aatggcaaac tggatgttgc
     1201 gaatagcgtc gagtttttcg aatcgctcgc caggttccag cccttccgct aaagcgacca
     1261 gcagattagt gataccagca gaagccgaga ggactactaa acgcacgttg gcatcagaaa
     1321 gcacaatatc agcgctgcgg ttcatggcgt caaaatcagc tacgctggta ccgccaaatt
     1381 tggagacaac aatttcagac ataactacct cgtgtcaggg gatccatttt cagccttggc
     1441 acaagggaag agcggaagac gggtgggcgc agagcgatac ttcgctacta ttttcaccca
     1501 gaagtgctcc accacttgcg aaacgcccga ctgcgaacgc ttctggtgac aacccagggg
     1561 attcagcccc tgtagccgat gatgaacgtg gccagccgtt caatcacctc ggcgatgcac
     1621 cccctcaggt gttatcacag gactggctcc tccaacaccg ttacttgggc aacgcgcctc
     1681 ttctggcctg cgctagcgca ggtagtacat ttataaataa agggtgagcg gggcggttgt
     1741 caacgacggg gtcatgcgga tttttcatcc actcctggcg gtcagtagtt cagctaataa
     1801 atgcttcact gcgctaaggg tttacactca acattacact aacggcacta aaaccatcac
     1861 atttttctgt gactggcgct acaatcttcc aaagtcacaa ttctcaaaat cagaagagta
     1921 ttgctaatga aaaacatcaa tccaacgcag accgctgcct ggcaggcact acagaaacac
     1981 ttcgatgaaa tgaaagacgt tacgatcgcc gatctttttg ctaaagacgg cgatcgtttt
     2041 tctaagttct ccgcaacctt cgacgatcag atgctggtgg attactccaa aaaccgcatc
     2101 actgaagaga cgctggcgaa attacaggat ctggcgaaag agtgcgatct ggcgggcgcg
     2161 attaagtcga tgttctctgg cgagaagatc aaccgcactg aaaaccgcgc cgtgctgcac
     2221 gtagcgctgc gtaatcgtag caataccccg attctggttg atggcaaaga tgttatgcca
     2281 gaagtcaacg cggtgctgga gaagatgaaa accttctcgg aagcgattat ctccggtgag
     2341 tggaagggtt ataccggcaa agcaatcact gacgtagtga acatcgggat cggcggttct
     2401 gacctcggcc cgtacatggt gaccgaagca ctgcgtccgt acaaaaacca cctgaacatg
     2461 cactttgttt ctaacgtcga tgggactcac atctcggaag tgctgaaaaa agtaaacccg
     2521 gaaaccacgc tgttcctggt agcatctaaa accttcacca ctcaggaaac catgaccaac
     2581 gcccatagcg cgcgtgactg gttcctgaaa gcggcaggtg atgaaaaaca cgttgcgaaa
     2641 cattttgcgg cgctctcaac caatgccaaa gccgttggcg agtttggtat tgatactgcc
     2701 aacatgttcg agttctggga ctgggtcggc ggccgttact ctttgtggtc agcgattggc
     2761 ctgtcgattg ttctctccat cggctttgat aacttcgttg aactgctttc tggcgcacac
     2821 gcaatggaca agcatttctc caccacgcct gccgagaaaa acctgcctgt cctgctggcg
     2881 ctgattggca tctggtacaa caatttcttt ggtgcggaaa ctgaagcgat tctgccgtat
     2941 gaccagtata tgcaccgttt cgcggcgtac ttccagcagg gcaatatgga gtccaacggt
     3001 aagtatgttg accgtaacgg taacgttgtg gattaccaga ctggcccgat tatctggggt
     3061 gaaccaggca ctaacggtca gcacgcgttc taccagctga tccaccaggg aaccaaaatg
     3121 gtaccgtgcg atttcatcgc cccggctatc acccataacc cgctctctga tcaccaccag
     3181 aaactgctgt ctaacttctt cgcccagacc gaagcgctgg cgtttggtaa atcccgcgaa
     3241 gtggttgagc aggaatatcg cgatcagggt aaagatccgg caacgcttga ctacgtggtg
     3301 ccgttcaaag tgttcgaagg taaccgcccg accaactcca tcctgctgcg cgaaatcact
     3361 ccgttcagcc tgggtgcgtt gattgcgctg tatgagcaca aaatctttac tcagggcgtg
     3421 atcctgaaca tcttcacctt cgaccagtgg ggcgtggagc tgggtaaaca actggcaaac
     3481 cgtattctgc cagagctgaa agatgataaa gaaatcagca gccacgatag ctcgaccaat
     3541 ggtctgatta accgctataa agcgtggcgc ggttaatcat catgcgtgcc tgatgccgat
     3601 atgtaggccg gataaggcgt ttacgccgca tccggcaacc ggagcctgat gccaatatcg
     3661 acattaatgc atagtatgag gagttaaata accttaaaaa caatgccgac aataagtcgg
     3721 cattgtttta tcagataaat ccccttgtct gtgatttaac ggaaatcata ccgtgaggtt
     3781 aatcctaaaa tagattttta atcgttgttt atttcggaga atacgcagat taattacttt
     3841 tgttttttat tttaagctta tgatttttat tgttatttaa atataagttg aaacttatat
     3901 ttgatattca ttccaattat cctaaaactc catcgctaat tccccgcgcc gtaattcgca
     3961 tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt
     4021 caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt
     4081 gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcaga gtcggcagca
     4141 acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg
     4201 ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc
     4261 acgaccacca gtacccagta ataaagtatg tatccccaaa ataatttgag tcattgcatc
     4321 tgtggcttga agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc
     4381 tctggagaag agtcgtgaag cgacctgcac tcattcttat ctgcctgcta ttacaggcct
     4441 gttcagccac gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag
     4501 gcgtacagct gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc
     4561 ttaatggcgg gccgcaattg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat
     4621 gggtcactca ggatcaagcc actcttgtca cacaacatgg tcgtctggtg aagactttgc
     4681 ttggcggcga caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg
     4741 cacaaattgt tgatggtgca acatggacgc gcacgatggg ctggaccgag taccagcagg
     4801 tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccgatacc gtcaaggtcg
     4861 gcagcgatga aaccctggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct
     4921 ggcataaccg ctactggatc gacagcgaag ggcaaattcg ccagtcggaa cagtatctcg
     4981 gcgcggatta tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca
     5041 aactattgtc gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt
     5101 caaggtgttc agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat
     5161 cgatctggta gggcaacctc gactggcaaa cagctggtgg cctggtgcgg tgattagcga
     5221 agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga
     5281 acagggcgca gattccagca ccgacgatgc cgccgccatt aacgccttac gccagcaaat
     5341 ccaagcattg aaagtgacag gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt
     5401 tgccgaacgc ggtaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccgcc
     5461 gtccacggtc acgttgttcg ggcttatcag ccatcctggc aatcagccat tcactccagg
     5521 tcgcgacgtg gcgagctatc tctctgacca gagcctgctc agcggtgcgg atcgcagcta
     5581 cgcgtgggtc gtttacccgg acggacgcac gcaaaaagcg ccggtggctt actggaacaa
     5641 gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcag attccgtctg
     5701 gagtgagacg cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc
     5761 ccaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc
     5821 tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga
     5881 ttattacaaa ctcccaccgc gcgtatggca cgggaagggg agttgagtct gaactatcgc
     5941 gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca
     6001 acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc
     6061 gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac
     6121 tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg
     6181 gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgctcgg cctgggctgg
     6241 ggatatctgg gcaccagcgg taatgtaaaa aatccgctct gttcagccag tgataaatat
     6301 tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac
     6361 ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg
     6421 aaactggagt atgaaggtaa taattatcag caggattttg ccgggaagct ggagcaaaaa
     6481 agtaagttta acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc
     6541 tatgaacgtg gcaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg
     6601 cgcccgtcct acatcgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt
     6661 ttgcagcatt cggtggtggc gaatcagtta acgttgctga aatacaacgc cggacttgcc
     6721 gatccgcaga tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat
     6781 cgtgattcgc gcgaagggat catccgtgct aatcggatcg tgatgaacga tctgccggat
     6841 gggatcaaaa cgatccgcat tacggaaaat cgccttaaca tgccgcaagt gacgacggaa
     6901 accgatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgacg
     6961 ctggcgcaca aacgcgtcga gccagtggtt ccgaagtcca ccgagcaggg ctggtatatc
     7021 gacaaatcac gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg
     7081 gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac
     7141 catctactga ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac
     7201 tacaccaatc caccgcagga ctcgcactta ccgcgcgttc gtacccatgt gcgcgagtat
     7261 gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac
     7321 ggcttctacg gtcaggtcta tggaggttat ctcgaaacca tgtttggcgg tgcgggggcc
     7381 gaagtgttgt atcgtccgct ggacagtaac tgggcgtttg gtctggatgc caactacgtt
     7441 aaacagcgcg actggcgtag tgcaaaagat atgatgaaat tcaccgacta cagcgtgaaa
     7501 accggacatc tgaccgccta ctggacgcca tctttcgccc aggacgtgtt ggtgaaagcc
     7561 agcgtcgggc agtatctggc aggggataaa ggcggcacgc tggagatcgc taaacgcttt
     7621 gacagcggcg tggtggtggg tggctatgcc acgatcacca acgtttcgaa agaggagtac
     7681 ggcgaagggg acttcaccaa aggcgtgtat gtctcggtac cgttggatct cttctcgtct
     7741 ggcccgacac gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag
     7801 caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt aaatttccgc
     7861 taagtcatgg gaaaggtgcc agttttcgca ctcactggca ccctcattct taatgacctg
     7921 ttgagccatc aggaacgcag ttcacccccg gtttacgatc tggtgtgtaa aggcgaaaac
     7981 attctgtctt cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca
     8041 tcattgggct ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac
     8101 cagcataagc aggaagaaca gagactgata acatcaaagc agcaaaataa ggcttcattt
     8161 ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg
     8221 cattgctggt ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg
     8281 gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc
     8341 ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga
     8401 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     8461 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     8521 cttggcctgc tgtgcctcgg cctgattttg gttgtcttcc tcggcaaaga aacggtgcat
     8581 ctggctgatg tgctgttcgc gccagaacaa accagcaaat atgagctggt agaaggactg
     8641 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     8701 tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc
     8761 atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     8821 gtgatcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg
     8881 ccgaaatggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     8941 tcgtttccgg ttcccagagc gcttccagct cctcaagggt tttacctttg gtttccggga
     9001 caaatttcca cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga
     9061 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     9121 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttaccacgaa
     9181 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     9241 aggcggcaac atagaacagc atcgacagta gcgccacaat acccgatgcc tgagtgtaaa
     9301 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     9361 gcttacgacc aaatttatcc acagtcataa ttgccagtac ggtaaaggtg aggttgataa
     9421 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     9481 cttccggcgc gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca
     9541 taacgccgat tacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     9601 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     9661 ttttgcgcag gatactttcc gtctgttctt gcttgccgcg tgacatcagc cagcgaggac
     9721 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     9781 acatataacg cctgccgtca gtattcagcc agctggcatc accggaacgg gcaataaaat
     9841 agtttacgca gtaaactaaa agttgcccga aaataatcgc aaactggtta aatgaaacca
     9901 gttttccgcg aatatgagct ggagccagtt ccgcaatata cattggtgaa agcattgagg
     9961 ctaaaccaac gccaataccg ccaataatac gataaataac aaattccggg acataacctg
    10021 ccagataaat aggtactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
    10081 cagaacctac accagaaata aaaaacagga cagcagaaat cttaagtgaa tcacgacggc
    10141 cgaagcggtt actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc
    10201 tggctacgca aaatcctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
    10261 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
    10321 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
    10381 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
    10441 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
    10501 gatcttaatt gtgataatta tcaaaaattg atgaaacaat actattgccg tgactcagag
    10561 cacgaaagag aattatcgta agtgggaaaa caaataacgt aaaaataata agttctatga
    10621 tgggaatatt aaccggcgaa cgattcagat tgcagacgaa agaaaaaaag gcgctccgtg
    10681 gagcgccgaa taatagtcac aagttgggat aacgtaagtt gagggtgcag cggcataaca
    10741 ttggcagaac aacatcttta acctttcaca ccacctgccg tcaggccgtt gaccagccag
    10801 cgttgagcca gcaagaagac gatggtgatc ggtaatgcag acatcacggc agcggcggca
    10861 aagtcacccc acaggtagtt ttgcgggttg aggtattgct gcatccccac ggccagggtg
    10921 tagctgttta cgtcacgcag taacagcgac gcgaccggaa cttcagtaat ggcagcgata
    10981 aacgacagga tgaataccac cgccagaatc ggtactgaca gcggcaacag gacaaggcgg
    11041 aaggcctgcc acggtgtcgc accatccagc gcagccgctt cttccagcga actgtcgatg
    11101 gtttcgaaat agcctttgat ggtccagaca tgcagcgcaa tcccacccag atacgcgaaa
    11161 attacgccgc cgtgagtatt caggccaatg aatggaatgt actcacccag acgatcaaac
    11221 aacgcataca acgcgaccag tgaaagtact gccgggaaca tctggaaaat cagcattcct
    11281 ttcagcagcg tcgctttgcc tggaaagcgc atacgggcga aagcgtaggc gcaggtggtg
    11341 gagagcgcca caatgccaat cgcggaaatc ccagcgacct ttaccgagtt ccacagccac
    11401 agcagtaccg ggaatggcgg tggcgtaatg cgaccatcag cctgttcaac gctaaaacct
    11461 aacgccagtt tccagtgatc ccaggagatt tgctccggga tcaggctacc ggtcgcgaag
    11521 tttccctgac gcagcgagat agcgacgacc atcagcagcg ggaacataat cgccgcgata
    11581 aaaagtagca gtagcaggtg agtaataaac aaacgtgctt tttgcgattt cggttggacc
    11641 attgccattt ttgttatctc ccttaatcaa acttcattcg cgtggctttc aggttcacta
    11701 tcgccagcgc acccaccagc aggaagatca gcgtggcaat tgctgccgcc agaccgaagt
    11761 cctgaccccc gccgccttca aaagcgatac ggtaggtgta gttaacaagc aggtcggtat
    11821 aaccggctgg cgtggtcgtg ccaagacgat ccgggccgcc gttggttaac agctgaatca
    11881 gcacgaagtt gttaaagtta aaggcgaagc tggcgatcat cagcggcgtc agcggtttaa
    11941 tcagcagcgg cagcgtaatc ttaaagaagt tctggaacgg acctgcgcca tccattgctg
    12001 aggcttcata caaatcgtcc ggaatcgctt tcagcaagcc catgcagagg atcatcatgt
    12061 acggataacc cagccaggta ttgacgataa tcagcatcgt gcgagcggtg gtcggatcgc
    12121 tgaaccaggc gggcttcacg ccaaacagcg cgctcaacat catgttgatt tcaccgaagc
    12181 tctggttaaa caatcctttg aaaatcaaga ttgaaatgaa cgatggcacc gcgtagggca
    12241 gaatcagcag gacgcgatag accgctttgc cgcgcaacgc ttcccactgc accagacatg
    12301 ccagaaccat gccgaccgcc accgttaaaa agacagtgat cagcgagaac accacggtcc
    12361 agacgaaaat ggcgaggaac ggtttctgaa tgccttcgtc ggtaaagacg cgggtaaagt
    12421 ttttccagcc ggtggtcacg gtgtaaccgg ggcttagctt ttcatccccc cagttgccgt
    12481 cggcggtaat ggactggtaa aagccaattt ggttattcgg acgatatttc acgccactct
    12541 gattattcgt caacgtgccg tcaccgtcga gtgtgtagag cggctgcgtg ccagaaaact
    12601 ggcgcaggga gctcatcatc actttgttgc catccggcag aatggcggta atgtcactca
    12661 gcgcctgacg attctgggta atcacgcgca gattcgcgcg ttcgccttcg ggctgggcgg
    12721 tcgtttcttt cagttgcagt ttttgctcgc cgccaaattt aaacgcgtcg gagaggtaat
    12781 ttttgccggt ttcgccgtcg ctgagcgcca gttgccactc atcgcccgcc gggtaaagac
    12841 caaagttata ggttttgcct gcttgccagg agcgatctaa caacacttcc tgcgcacgtt
    12901 caaaagtcag ctggttagtg ctgctgtagt tggtgaaggc aatggcgatg gtgcagacca
    12961 gagggaagag gacgaataat cccattccag ccattcccgg gtaaacatag cgccaggcgt
    13021 aggctttacg attggcgaaa atatacagcc ccgctgaact caatatcagc gtggtaatgg
    13081 cgaacaggta ttccccttgt gcgtacatta aaacaacaag gtaacccacc agcaggccga
    13141 gcagacctag cactgaccat ttcagcgcgt cgctttacca ccaatgtttc tttttaatga
    13201 catccatggg gttcttcctc attccaggac ggataaggct ttcacgcctt atccgacaac
    13261 aactgcctga tgcgacactg atgcgtctta tcaggcctac atgcgtttca gttttgtagg
    13321 ccggacaagg cgttcacgcc gcatccggca tttcacagca ttacttggtg atacgagtct
    13381 gcgcgtcttt cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag
    13441 tacgtacggc ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct
    13501 gggcgttttc catggtggcg gcaatacgtg gatctttcgc caactcttcc tcgtaagact
    13561 tcagcgctac ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcatcagtca
    13621 gcagatagtt ttcgaggaac tctttcgcca gctctttgtt cggactggcg gcgttaatac
    13681 cggtgctcag cacgccaacg aacggtttag atggttgacc cttgaaggtc ggcagtaccg
    13741 ttacaccata attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg
    13801 tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat
    13861 tcatgtgttt gtttttaatc aggtcaacca ggaaggtcag acccgctttc gcgccagcgt
    13921 tatccacgcc cacgtcttta atgtcgtact tgccgttttc atacttgaac gcataacccc
    13981 cgtcagcagc aatcagcggc caggtgaagt acggttcttg caggttgaac atcagcgcgc
    14041 tcttaccttt cgctttcagt tctttatcca gcgccgggat ctcttcccag gtttttggcg
    14101 ggttcggcag caggtctttg ttataaatca gcgataacgc ttcaacagcg attgggtaag
    14161 caatcagctt gccgttgtaa cgtacggcat cccatgtaaa cggatacagc ttgtcctgga
    14221 acgctttgtc cggggtgatt tcagccaaca ggccagattg agcgtagcca ccaaagcggt
    14281 cgtgcgccca gaagataatg tcagggccat cgccagttgc cgcaacctga gggaatttct
    14341 cttccagttt atccggatgc tcaacggtga ctttaattcc ggtatctttc tcgaatttct
    14401 taccgacttc agcgagaccg ttatagcctt tatcgccgtt aatccagatt accagtttac
    14461 cttcttcgat tttggcgaga gccgaggcgg aaaacatcat cgtcgttaat gcggataatg
    14521 cgaggatgcg tgcacctgtt tttattttca taatctatgg tccttgttgg tgaagtgctc
    14581 gtgaaaacac ctaaacggac tctagttcct ttatacggca acctctttcc atcctccttg
    14641 cccctacgcc ccaccgttgc tttgtgtgat ctctgttaca gaattggcgg taatgtgggg
    14701 atgcgcacat aaaatcgcca cgatttttgc aagcaacatc acgaaattcc ttacatgacc
    14761 tcggtttagt tcacagaagc cgcgttctca tcctcccgcc tcctccccca taaaaaagcc
    14821 agggggtgga ggatttaagc catctcctga tgacgcatag tcagcccatc atgaatgttg
    14881 ctgtcgatga caggttgtta caaagggaga agggcatggc gagcgtacag ctgcaaaatg
    14941 taacgaaagc ctggggcgag gtcgtggtat cgaaagatat caatctcgat atccatgaag
    15001 gtgaattcgt ggtgtttgtc ggaccgtctg gctgcggtaa atcgacttta ctgcgcatga
    15061 ttgccgggct tgagacgatc accagcggcg acctgttcat cggtgagaaa cggatgaatg
    15121 acactccgcc agcagaacgc ggcgttggta tggtgtttca gtcttacgcg ctctatcccc
    15181 acctgtcagt agcagaaaac atgtcatttg gcctgaaact ggctggcgca aaaaaagagg
    15241 tgattaacca acgcgtcaac caggtggcgg aagtgctgca actggcgcat ttgctggatc
    15301 gcaaaccgaa agcgctctcc ggtggtcagc gtcagcgcgt ggcgattggc cgtacgctgg
    15361 tggccgagcc aagcgtattt ttgctcgatg aaccgctctc caacctcgat gctgcactgc
    15421 gtgtgcaaat gcgtatcgaa atctcccgtc tgcataaacg cctgggccgc acaatgattt
    15481 acgtcaccca cgatcaggtc gaagcgatga cgctggccga caaaatcgtg gtgctggacg
    15541 ccggtcgcgt ggcacaggtt gggaaaccgc tggaactgta ccactatccg gcagaccgtt
    15601 ttgtcgccgg atttatcggt tcgccaaaga tgaacttcct gccggtaaaa gtgaccgcca
    15661 ccgcaatcga tcaagtgcag gtggagctgc cgatgccaaa tcgtcaacaa gtctggctgc
    15721 ctgttgaaag ccgtgatgtc caggttggag ccaatatgtc gctgggtatt cgcccggaac
    15781 atctactgcc gagtgatatc gctgacgtca tccttgaggg tgaagttcag gtcgtcgagc
    15841 aactcggcaa cgaaacccaa atctatatcc agatcccttc cattcgtcaa aacctggtgt
    15901 accgccagaa cgacgtggtg ttggtagaag aaggtgccac attcgctatc ggcctgccgc
    15961 cagagcgttg ccatctgttc cgtgaggatg gcactgcatg tcgtcgactg cataaggagc
    16021 cgggcgttta agcaccccac aaaacacaca aagcctgtca caggtgatgt gaaaaaagaa
    16081 aagcaatgac tcaggagata gaatgatgat tactctgcgc aaacttcctc tggcggttgc
    16141 cgtcgcagcg ggcgtaatgt ctgctcaggc aatggctgtt gatttccacg gctatgcacg
    16201 ttccggtatt ggctggacag gtagcggcgg tgaacaacag tgtttccaga ctaccggtgc
    16261 tcaaagtaaa taccgtcttg gcaacgaatg tgaaacttat gctgaattaa aattgggtca
    16321 ggaagtgtgg aaagagggcg ataagagctt ctatttcgac actaacgtgg cctattccgt
    16381 cgcgcaacag aatgactggg aagctaccga tccggccttc cgtgaagcaa acgtgcaggg
    16441 aaaaaacctg atcgaatggc tgccaggttc caccatctgg gcaggtaagc gcttctacca
    16501 acgtcatgac gttcatatga tcgacttcta ctactgggat atttctggtc ctggtgccgg
    16561 tctggaaaac atcgatgttg gcttcggtaa actctctctg gcagcaaccc gctcctctga
    16621 agctggtggt tcttcctctt ttgccagcaa caatatttat gactatacca acgaaaccgc
    16681 gaacgacgtt ttcgatgtgc gtttagcgca gatggaaatc aacccgggcg gcacattaga
    16741 actgggtgtc gactacggtc gtgccaacct gcgtgataac tatcgtctgg ttgatggcgc
    16801 atcgaaagac ggctggttat tcactgctga acatactcag agtgtcctga agggctttaa
    16861 caagtttgtt gttcagtacg ctactgactc gatgacctcg cagggtaaag gtctgtcgca
    16921 gggttctggc gtcgcgtttg ataacgaaaa atttgcctac aatatcaaca acaacggtca
    16981 catgctgcgt atcctcgacc acggtgcgat ctccatgggc gacaactggg acatgatgta
    17041 cgtgggtatg taccaggata tcaactggga taacgacaac ggcaccaagt ggtggaccgt
    17101 tggtattcgc ccgatgtaca agtggacgcc aatcatgagc accgtgatgg aaatcggcta
    17161 cgacaacgtc gaatcccagc gcaccggcga caagaacaat cagtacaaaa ttaccctcgc
    17221 acaacaatgg caggctggcg acagcatctg gtcacgcccg gctattcgtg tcttcgcaac
    17281 ctacgccaag tgggatgaga aatggggtta cgactacaac ggcgatagca aggttaaccc
    17341 gaactacggc aaagccgttc ctgctgattt caacggcggc agcttcggtc gtggcgacag
    17401 cgacgagtgg accttcggtg cccagatgga aatctggtgg taatagcaaa acctgggccg
    17461 gataaggcgt ttacgccgca tccggcataa aaacaggttg tcattatctg aaaggggcga
    17521 aagcccctct gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt
    17581 ttgaggtgaa aacaatgaaa atgaataaaa gtctcatcgc cctctgttta tcagcagggt
    17641 tactggcaag cgcgcctgga attagccttg ccgatgttaa ctacgtaccg caaaacacca
    17701 gcgacgcgcc agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc
    17761 aatctaaaac ccagaccacc caactggcga ccggcggcca acaactgaac gttcccggca
    17821 tcagtggtcc ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctgacgc
    17881 tgaccagcga agtgaacaaa caaaccagcg tttttgcgcc gaacgtgctg attcttgatc
    17941 agaacatgac cccatcagcc ttcttcccca gcagttattt cacctaccag gaactaggcg
    18001 tgatgagtgc agatcggctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa
    18061 aactttatgt tctggtcttt accacggaaa aagatctcca gcagacgacc caactgctcg
    18121 acccggctaa agcctatgcc aaaggcgtcg gtaactcgat cccggatatt cccgatccgg
    18181 ttgctcgtca taccagcgat ggcttactga aactgaaagt caaaacgaac tccagctcca
    18241 gcgtgttggt aggaccacta tttggttctt ccgctccagc tccggttacg gtaggcaaca
    18301 cggcggcacc agctgtggct gcacctgctc cggtgccggt gaagaaaagc gagccgatgc
    18361 tcaacgacac ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg
    18421 ttgataaggc gttaaaactg cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt
    18481 ccacctttat cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc
    18541 aacaactggt gcgtctcctg gcgcaccttt ttttatgctt ccttcctggg atatgagcga
    18601 ttttttatag tacttcactt cttcttcact aagaatgtcc attatttcaa tgcattatca
    18661 gagattcttt tcctttcgcc ggtagtgtct ggacgctcag gctacttttc caggttattt
    18721 tatttctgta ttacagaggt tttatgatag gtcatatcac aaattctggc ggcaataact
    18781 ctttgatgaa acatgatgtg gtgcaaggaa ataatatagt agatcttgat ttactacgta
    18841 atttcaatgg ggtgacaggt ttaaacagag attactttat ttatatcagt gatatatttt
    18901 taaatataaa acaacggaac gaaaaaaatc atgcaataaa tatgttacgt gaaatctcaa
    18961 ttagtaatga tactataagt gtaaaattct acagaaatga aaaaatagaa tgcgcttgta
    19021 attttctgat ggataaagat gcgcaggggt atatcgacct gtctgatttt gatttaacaa
    19081 attgtcattt taaaggtgac gttatttcta aggtgtcttt tttatcatca aatctacaac
    19141 atgtaacttt cgaatgtaaa gaaattgaga attgcaattt tactaaggca acagttgata
    19201 atgtcatatt taaatgtcga cgtttacaca atgtgatttt tctcaaaacg agtggcgaat
    19261 gtgttgattt tagccaaaat attcttgata cagttgactt ctcgcagagt caacttggtc
    19321 atagtaattt tcgcgaatgt cagattagaa attcaaactt cgataattgt tatctttacg
    19381 cttcgcactt caccagagca gagtttctgt ctgccaaaga aatatcattt attaaatcga
    19441 atttgacagc tgttatgttt gatcatgtgc gaatgtcgac agggaatttt taagattgca
    19501 ttacagaaca attggaatta actattgatt attcagatat atttgggaat gaagatctcg
    19561 atggttatat caataacatt ataaaaatga ttgatacatt gccagataat gcaatgatat
    19621 tgaaatccgt tctggcagta aaactggtga tgcaattaaa aattctcaat attgttaata
    19681 aaaactttat tgagaatatg aagaaaacat ttagccattg tccttatata aaagatccaa
    19741 ttatacgtag ttatatacat tctggggaag atgacaagtt cgataatttt atgcgtcaac
    19801 atcgattcag taaggtgaat ttcgataccc aacagatgat ccattttatt aacagattta
    19861 atatgaataa agggctggtt gataaaaata acaatttttt tatccaactt atcgatcagg
    19921 ccttacgatc aacggatgat atgatcaaag caaatgcctg gtatctttat aaagagtgga
    19981 ttcgtagtga tgatgtttca cctatattta tagaaactga agaaaagtta agaaccttta
    20041 acactaataa attaacacga aacgataata tatttatcct gttctcctct gtcgatgatg
    20101 ggcctgttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata
    20161 ccaattggaa ttccacgtgt atctataaat ccagacataa gatgttgcct attaatctta
    20221 ctcaggaaac acttttcagc tccaaatctc atggtaaata tgcgcttttc ccaattttta
    20281 ctgcgagttg gcgagctact cgtataaaga ataaaggtat ttaagtaaag gaaaacatca
    20341 ccgttcctag catcatgggc ggtgatgccc ctatggttgc cctcgccagc acgggcatcg
    20401 gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt ttttgttgcg
    20461 cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct
    20521 ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc
    20581 tgcgctattt taaagagatc cctgccctgg atccgcaact gctcgactgg ctgttgctgg
    20641 aggattccat gacaaaacgt tttgaacagc agggaaaaac ggtgagtgtg acgatgatcc
    20701 gcgaggggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg ccgaaagagt
    20761 ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc
    20821 gtaccgtcgt tcctgtatca acattaagcg ggccggaact agcgttacaa aaattgggta
    20881 aaacgccgtt aggacgctat ctgttcacat catcgacact aacccgggat tttatcgaga
    20941 taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc
    21001 tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg
    21061 agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc
    21121 caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg
    21181 gcgttcctca gctctggatc atggcggtgt ttgtcgcggg tgtctggctg atgcgcgctg
    21241 ctggttgcgt ggtgaatgat tatgctgatc gcaagtttga tggtcatgtt aagcgcacgg
    21301 cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttgtag
    21361 tgctggtact gatttcgttt ttactggttc tgacgctgaa tacgatgacc attctgttgt
    21421 cgattgccgc tctggcattg gcgtgggtgt acccgtttat gaagcggtat acccatctcc
    21481 cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gccgctgtga
    21541 gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg
    21601 cttacgacac gcagtatgcg atggttgacc gcgatgatga tgtgaagatt ggcattaaat
    21661 ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg cagattggcg
    21721 tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga tattactggt
    21781 caattgtggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc
    21841 gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta ctgtttttag
    21901 gactggcaat gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct
    21961 ttcttctggg ttgcctgatg cgttgcgctt ctcaggccta cccggtgaat cgcaatttat
    22021 tgaatttgca ggtcatggta ggccggataa ggcgttttcg ccgcatccgg caattctctc
    22081 tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa
    22141 ctccgccagc aactgataaa ccttcatcgt ttctgctggt tcggcatcgc cgctatcgct
    22201 gatataccct tcatcacgca gcgtcagcac cagagaactg aacaccgcct tgtcgaagaa
    22261 ctccggcgcg ttgatgccgt gcagcacgga gagacgttgc gcaacggtgc ggctctcttt
    22321 ctccagcgta ccgcggttga tcgacgggtt ggcactcaac aaccagaagg tgatggcata
    22381 acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt agggtgcgag aatgcgccgg
    22441 gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc
    22501 cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt
    22561 cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg
    22621 ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg
    22681 atagtaggtc atcagcaccg cttgctcgcg tggcagaatg atgatgtcgc cgatagtgtc
    22741 tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc
    22801 tgaaggaacg gtagagtccg tggagtaagg tacgttgcgc atcagatcca ggtagcagtt
    22861 gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt
    22921 acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc
    22981 agcaatatta ttgaccgtcg gcgttaacca ggccggacgc accgcttcga tgggatcgat
    23041 agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc
    23101 gaagttgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca gcatctgcgg
    23161 caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat
    23221 gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag
    23281 catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtgcg
    23341 ggaacgaccg ccttccacga agtactcgac ggaataacca cggctgaaca gttcgccgag
    23401 atactcacgg aaaacggtgg aataaagttt attgccttta aaagtacggc gaataaagaa
    23461 cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga tcccggcggc
    23521 gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagtagca ggtagtccat
    23581 gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca gctggcgaac
    23641 gcgctcggcg ttatggacgt tgatgccctg ataaaatcgg ttccaggtga agcccagaat
    23701 gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagcgc
    23761 aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac
    23821 cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcag
    23881 acgtgggcct acggcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag
    23941 tttctgagcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca gcgacactga
    24001 cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acagcgaaaa atttctgtac
    24061 gccgttaagc atacgcagcg gcgggttcac ttcgcctttt tcacgccccg gcgcgcgacc
    24121 aaacatcacc gatactggca ccatctgcac atccagattt gggttgctac ggtgcaaatc
    24181 gagatagtcg tggaaaagct taatagactc ttctttcggc gtgtaatagg tgaatacacg
    24241 cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc
    24301 taacgggtca ggcaaatcat gtgccagaca ctgggcgcgc aacgtcagca aatctgcttt
    24361 cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca gttccggggc
    24421 aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt
    24481 gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa
    24541 tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg
    24601 cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat
    24661 gtaaaaaggt tctttcaatg gccaataata caactggatt cacccgaatt atcaaagctg
    24721 ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg
    24781 aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcaatta
    24841 cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg
    24901 ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcaa
    24961 aagatatggg atccgccgcg gtgctgattg ccattatcgt cgccgtgatt acctggtgca
    25021 tcctgttatg gtcgcatttt ggataaccct tccagatttc gataaatctc tggtttattg
    25081 tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagtata actgtatata
    25141 cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
    25201 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
    25261 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt
    25321 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
    25381 ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca gcatattgaa
    25441 ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc
    25501 agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc agtgcataaa
    25561 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt
    25621 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
    25681 ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggggtt
    25741 attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgct tggcgtcgcg
    25801 gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat
    25861 aaagcactct ggcatctcgc cttacccatg attttctcca atatcaccgt tccgttgctg
    25921 ggactggtcg atacggcggt aattggtcat cttgatagtc cggtttattt gggcggcgtg
    25981 gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt acgcatgagc
    26041 accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg
    26101 ctggtgcaac cgttgctgtt ggcgttgggg gccggggcgt taattgcgct gttgcgtacg
    26161 ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcggtcct ggaacaggcg
    26221 cggcgctttc ttgaaatccg ctggttaagc gctccggcgt cgctggcgaa tctggtatta
    26281 ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc
    26341 aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctgcatat gaacgtgcag
    26401 ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg tctgctaatg
    26461 gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac tgcctggcga
    26521 ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc gctgttgttg
    26581 caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga cattatcgct
    26641 gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgcttga tggttttgcc
    26701 tacgcggttg aagcgcactc cggtcaggca tacggtgcgc gcgacggtag ccagttgctg
    26761 gatgtatggc gggcagcgtg ccgccagtcg gggattgtag cgttactatt ttcggtggtt
    26821 tatttgctgg caggggaaca catcattgcg ttgctgacgt cgttaaccca gattcagcag
    26881 ctggctgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt ctggtgttat
    26941 ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg
    27001 gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg taatcatggt
    27061 ttgtggctgg cattaaccgt ctttctggcg ttgcgcgggc tttctctggc ggctatctgg
    27121 cggcgtcact ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa aattctgaat
    27181 aaattatcct aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac
    27241 gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg
    27301 taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga
    27361 tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg
    27421 ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg
    27481 ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt
    27541 ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac
    27601 ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc
    27661 catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacact cttctttcac
    27721 cgcgccgcag cgatcgcaaa taaacatggc tgacgtatgc gtgggctggt cgaacagatg
    27781 acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa
    27841 atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagtaaatc
    27901 aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag
    27961 gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct gcgctaataa
    28021 ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac
    28081 tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg ttgattttct
    28141 gttttgtagg taaggtgttg tgttgcctta tcgtaccatt atcaacacga taataattaa
    28201 taattcattt ttaaataaat tgcttcaagt taactcgcta tttatttaac tgggtttaaa
    28261 cgcatacctg tagttcagaa attatgagcg atttttgata gtgaccagat ggcaaaagaa
    28321 ttataaggag gacacggtac gacaaaaaag gtagatgaat catgttgaag ataatacccg
    28381 gtgcaactgg atactttaat aaaacactca attcaaatca attcgaaaat aaagatgcaa
    28441 ccaaagataa attggataat agaggttcaa tcaaaggtaa attaaataat atatatggta
    28501 aatctattga ttatgcagcg ctgcgtcatc gggatataat tattgcaaaa atagatttgt
    28561 ttattcaacg aatcacgcat aatttatggc atgcacgtaa aaaaatgtgt ttttaattga
    28621 gcacataaat gatctcaaaa tgtgggtcaa taagtatact gacgattgca ctgatgagga
    28681 tttaaacgat cgtgacttta ttgcatcagt tgttgaccgg gctatttttc atttcgcgat
    28741 taatagtata tgtaatcctg gggataataa agatgcaatg cccattgaac aatgtacttt
    28801 tgatgtagaa actaagaatg gccttccctc cacggttcag ctattttatg aggaatctaa
    28861 ggataatgaa cctttagcga atatacattt tcaagcaata ggttctggtt ttttaacgtt
    28921 tgttaatgcc tgccaggaac atgatgacaa cagcttaaaa ttatttgctt cgctgttaat
    28981 ttcactttca tattctagtg cctacgcaga tttatcagaa acagtgtata ttaatgaaaa
    29041 taatgagagc tacctgaaag ctcagtttga aaaattatat caacgtgata tgaagaagta
    29101 cctgggagag atgaagcgtc tggcagatgg gggagaaatg aattttgatg gctatctgga
    29161 taagatgtca catctggtga atgaaggaac gctcgatcct gatattttaa gcaaaatgcg
    29221 agatgctgca ccacaattaa ttagcttcgc gaagtcgttt gacccaacct caaaggaaga
    29281 gattaaaata cttacagaca cttctaaatt aatttatgac ttgttcgggg ttaaatcgga
    29341 gaaataatat gtgaagttct tcgatagtat ggaaggcatt atataaaagg acccaatatt
    29401 tattgggttc ttttttctct atcaatgcta ttagcaggga gatatatcac cagagtttaa
    29461 tgtgtgattt tttatttatc gtcgaacctg gattgtttat cattggcctt aacaaagtta
    29521 acggctaata agattatttc catcacttcg tgagagcttc atgccttgag aggatctcaa
    29581 ttttcttttg caatgagaca ggcgcttcct gttgttatgg tatagtaccc cgctattgag
    29641 cctcctgaac agtgaagctg aataacataa ccccatgata tatcgataaa ataatctcta
    29701 catttgaaaa tgcacggtaa ttctgaaatg caaaaaatca accaaaccag cgcaatgcct
    29761 gaaaaaactg acgttcactg gagtggtcgg tttagtgttg caccaatgct cgactggacg
    29821 gacagacatt gccgctattt cttgcgtctg ctttcccgca atacgttgct ttataccgaa
    29881 atggtgacca caggggcgat aattcacggt aaaggtgatt atctggcgta cagtgaagaa
    29941 gaacatccgg tagcgttgca actgggcggt agcgatccgg cggcgctggc gcagtgtgca
    30001 aagctggcag aagcgcgcgg atatgatgag atcaacctga atgtcggctg cccgtctgac
    30061 cgggtgcaga acggcatgtt tggtgcgtgt ctgatgggta atgcgcagct ggttgccgac
    30121 tgcgtgaaag cgatgcgcga tgtggtgtcg attccggtga cggtgaaaac gcgtattggc
    30181 atcgacgacc aggacagcta tgaatttctc tgcgatttca tcaataccgt ttccggcaaa
    30241 ggcgagtgtg agatgttcat catccacgca cgtaaagcct ggctttcggg gttaagcccg
    30301 aaagaaaacc gtgaaatccc gccgctcgat tatccgcgtg tgtatcaact gaagcgtgac
    30361 ttcccgcatc tgacaatgtc gattaacggt ggtatcaagt cgctggaaga agctaaagcg
    30421 catttgcaac atatggatgg cgtgatggtc gggcgcgagg cgtatcagaa tccgggtatt
    30481 ctggcggcgg tagaccggga gatctttggt tcctcggata ccgatgccga tccggtggca
    30541 gtagtgcgtg ccatgtatcc gtacattgag catgaactca gccaggggac gtatctcggc
    30601 catattatcc ggcatatgct gggtttgttc cagggtattc ctggcgcgcg gcagtggcgg
    30661 cgttatttaa gtgaaaatgc ccataaagcg ggtgctgaca ttaacgtgct ggaacacgcg
    30721 ctcaaactgg tggcggataa gcgttaactt ttcaccaaaa agtagtcaaa ttcaccacgc
    30781 cctgcgcacc gtcgcggggc gttttgctgt taaatcaata gattattttt ggcatgattc
    30841 ttgtaatgcc agcaagagat ttcatatttg ggagagcatc atgctggaac tactttttgt
    30901 gattggcttt tttgtcatgc tgatggtcac cggcgtttcg ttgctgggca ttatcgccgc
    30961 gctggttgtg gcgacggcca ttatgttcct cggcggtatg ctggcattga tgattaagtt
    31021 gctgccgtgg ttactactgg cgattgcggt ggtgtgggtt attaaggcga ttaaagcacc
    31081 aaaaatgccg aaatatcagc gttatgaccg ctggcgttac taagggattg tgcggatgat
    31141 cacaacctaa ggttttatcc ttagaacaaa ataggaattg ataatcaaat ctgtcactat
    31201 tgcgcctcta acagattcat cgtgctgtac cctacataca gccgaactat aaaaagaaag
    31261 ggcttcccag gtggaagccc tatttctttt atggaatcag caggctggaa ccttgcgtcg
    31321 cccggctttc cagaatctca tgcgcacgct gcgcatcctt cagcggatat ttctgctgct
    31381 cggcgacatc gaccttaatc acaccgctgg caatcaaaga gaacagttca ttactggcct
    31441 cggttaattc ctcccgcgtg gtgatatagc cttgcaggga agggcgtgtc acatacaacg
    31501 agcctttttg attgagaatg cctaagttca caccggtaac cgcacctgat gagttgccaa
    31561 aactgaccat taagccgcgg cgttgcaggc aatccagcga ccgttcccag gtgtctctgc
    31621 ccacggaatc gtacactacg cgcactttct taccgccggt gatctctttc aatcgctcga
    31681 ccagattctc ttcacgatag ttaataacct gccacgcgcc ggcttttagc gcgctctgcg
    31741 ctttttgcgc ggttcctacg gtgccgatga gtttcgcgcc cagggctttt gcccactggc
    31801 aggcaattaa gccaatgccg ccagccgctg cgtggaacag gaactgttca tcgggtttaa
    31861 tttcataggt tttgcgcagc agataataaa ccgttaagcc tttcaggaag gatgccgcag
    31921 cttgctcaaa agaaattgcc gcaggcagaa tcgccgcttt atccgcatta atgttatgca
    31981 cagagctgta agcgcctaac gccgactgcg catagactac atgatcgcca gccttaatat
    32041 gctttacacc actgccgact ttactcacga tgcctgccgc ttcggtacct aatccgctgg
    32101 gtagcgatgg cggcgggtaa aggccgctgc ggatatacgt gtcgataaaa ttgatgccga
    32161 tggctttatt ttcgacctgg atttcattct ccgccggatc ggcaggagtg aactctacgg
    32221 cttgaagtac ttccgggcca ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc
    32281 cagaacgtat cgtcagggtc tgcttcatat ggtaaagttt cgacccattc tttatttcgg
    32341 taactccatt cactatggca ggaaataaac ccttcaacaa acagcaggct gaaccccgcg
    32401 aacgcgatcc acaagttgcc gggctgaaag tgcctccgca ctcgatcgaa gcggagcagt
    32461 cggtgttggg cggtttaatg ctggataacg aacgctggga tgatgtagcc gagcgtgtgg
    32521 tagcagacga tttttacacc cgcccacacc gtcatatctt tactgaaatg gcgcgtttgc
    32581 aggaaagcgg tagtcctatc gatctgatta cccttgcgga atcgttggaa cgccaggggc
    32641 aactcgatag cgtcggtggt tttgcttatc tggcagagct gtcaaaaaat acgccaagtg
    32701 cggcgaacat cagtgcttat gcggacatcg tgcgtgaacg tgccgttgtc cgtgagatga
    32761 tctcggttgc gaatgagatt gctgaagccg gttttgatcc gcaggggcgt accagcgaag
    32821 atctgctgga ccttgctgaa tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag
    32881 acgaagggcc gaagaacatc gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt
    32941 tgtttcagca gccacacgat ggcgttaccg gggtaaacac cggttatgac gatctcaaca
    33001 aaaaaaccgc tggcttgcag ccgtcggatt tgatcatcgt cgccgcgcgt ccgtcgatgg
    33061 gtaaaacaac atttgcgatg aacctcgtcg aaaacgcggc aatgttgcag gataaaccag
    33121 tacttatctt ctcgctggag atgccatcag aacagatcat gatgcgttct ctggcgtcgc
    33181 tgtcgcgcgt tgaccagact aaaatccgta ccgggcagct cgatgatgaa gactgggcgc
    33241 gcatttccgg caccatgggt attttgctcg aaaaacgcaa tatctatatc gatgactctt
    33301 ccggcctgac gccaacggaa gtgcgttccc gcgcacgccg tattgcccgt gaacacggcg
    33361 gcatcgggct tatcatgatc gactacctgc aactgatgcg cgtaccggcg ctttccgata
    33421 accgtacgct ggaaattgca gaaatctccc gctcgctgaa agcactggcg aaagaactga
    33481 acgtgccggt ggtggcgctg tcccagttga accgttctct ggaacaacgt gccgacaaac
    33541 gtccggtcaa ctccgacctg cgtgaatctg gctctatcga gcaggatgcg gacttgatca
    33601 tgtttatcta tcgtgatgag gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa
    33661 ttattatcgg taaacaacgt aacggcccaa tcgggacggt acgcctgacc tttaacggtc
    33721 aatggtcgcg cttcgacaac tatgcggggc cgcagtacga cgacgaataa taattatttt
    33781 atgaattagg taattaaagc aaacaattat caaggaacac aaatgcaagc ggcaactgtt
    33841 gtgattaacc gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcacctgcc
    33901 agtaaaatgg ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg
    33961 cgaacgctcc ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg
    34021 cgtgcggggg gaatcaccaa acctgtactg ttactcgaag gcttttttga tgccagagat
    34081 ctgccgacga tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct
    34141 gcgctggaag aggctagctt ggacgagccg gttaccgtct ggatgaaact cgataccggt
    34201 atgcaccgtc tgggcgtaag gccggaacag gctgaggcgt tttatcatcg cctgacccag
    34261 tgcaaaaacg ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca
    34321 aaatgtggcg caaccgagaa acaactcgct atctttaata ccttttgcga aggcaaacct
    34381 ggtcaacgtt ccattgccgc gtcgggtggc attctgctgt ggccacagtc gcattttgac
    34441 tgggtgcgcc cgggcatcat tctttatggc gtctcgccgc tggaagatcg ctccaccggt
    34501 gccgattttg gctgtcagcc agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag
    34561 cataaagccg gagagcctgt tggttatggt ggaacctggg taagcgaacg tgatacccgt
    34621 cttggcgtag tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg
    34681 ccagtgctgg tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc
    34741 tgcgtagact taggtccaca ggcgcaggac aaagccgggg atccggtcat tttatggggc
    34801 gaaggtttgc ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatt
    34861 acgcgcctga cttcaagggt cgcgatgaaa tacgtggatt aatcgttctg taatatttga
    34921 ttgtctgtgc cggatgcggc gtgaatgcct tatccggcca ataaaatcct aaaaattcaa
    34981 taagttgatg ttctttcatg ctcttataaa ggtcgtgcct ctggcggatg tacgtttatc
    35041 attagtctca ctctgttgct aattgccgtt cgctcctgaa catccactcg atcttcgcct
    35101 tcttccggtt tattgtgttt taaccacctg cccgtaaacc tggagaacca tcgcgtgttt
    35161 caaaaagttg acgcctacgc tggcgacccg attcttacgc ttatggagcg ttttaaagaa
    35221 gaccctcgca gcgacaaagt gaatttaagt atcggtctgt actacaacga agacggaatt
    35281 attccacaat tgaaagccgt ggcggaggcg gaagcgcgcc tgaatgcgca gccccatggc
    35341 gcttcgcttt atttaccgat ggaagggctt aacagctatc gccatgccat tgcgccgctg
    35401 ctgtttggtg cggaccatcc ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt
    35461 ggcggctcag gggcattgaa agtgggcgcg gatttcctga aacgctactt cccggaatca
    35521 ggcgtctggg tcagcgatcc tacttgggaa aaccacgtag caatattcgc cggggctgga
    35581 ttcgaagtga gtacttaccc ctggtatgac gaagtgacta acagcgtgcg ctttaatgac
    35641 ctgttggcga cgctgaaaac attacctgcc cgcagtattg tgttgctgca tccatgttgc
    35701 cacaacccaa cgggtgccga tctcactaat gatcagtggg atgcggtgat tgaaattctc
    35761 aaagcccgcg agcttatccc attcctcgat attgcctatc aaggatttag tgccggtatg
    35821 gaagaggatg cctacgctat tcgcgccatt gccagtgctg gattacccgc tctggtgagc
    35881 aattcgttct cgaaaatttt ctccctttac ggcgagcgcg tcggcggact ttctgttctg
    35941 tgtgaagatg ccgaagccgc tggccgcgta ctggggcaat tgaaagcaac agttcgccgc
    36001 aactactcca gcccgccgaa ttttggtgcg caggtggtgg ctgcagtgct gaatgacgag
    36061 gcattgaaag ccagctggct ggcggaagta gaagagatgc gtactcgcat tctggcaatg
    36121 cgtcaggaat tggtgaaggt attaagcaca gagatgccag aacgcaattt cgattatctg
    36181 cttaatcagc gcggcatgtt cagttatacc ggtttaagtg ccgctcaggt tgaccgacta
    36241 cgtgaagaat ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc cgggttaaat
    36301 acggcaaatg tacaacgtgt ggcaaaggcg tttgctgcgg tgatgtaatg caggaaagca
    36361 ggctggagtt acccagcctg cagtgaaatt aaactgtcgt cgctttcact atttctttgt
    36421 agatgatttt tttgatgccg ttgttctacg tgagagataa taaaacgttg tcagttcttt
    36481 tattgttaag tttatcccaa ttatctggaa ttcctttccc tattttttcg tgtggagtaa
    36541 taatttctgc acatctaacc attattaaat ttaataataa gtatcgggtt tgttacgata
    36601 gaatatttct atatcctcaa gaatatgctt tgccttattg cctgtgactt tctgagattc
    36661 aattatagta tcgttaaatt ctaatgttaa agagaactct tttttccgct ttgtgtaagt
    36721 ttaaccttat attgattacc aatatcaaat aaaaaggtcc tgtaatgaga ataatcacct
    36781 ttaacctcct cggcaaaaca gcaaagcgtg caaatataca taaagtcttt cttacccctg
    36841 attttgttaa ctaaatcaat aaatgcgtga aaaactttac ttgcaataga acttgatact
    36901 tcttctccag gtctacaaaa atcttttgca atttttaact gtgtttctat gtataaaaag
    36961 aaatctttgg cataagagat atctttatta tttagtgcat ctagctcaac aggtaaagat
    37021 gtcgttttgt tgctattcac atatgatata ttcatatcat catctccatc gcgagtaata
    37081 aaattaatca ccattgtagg gtagggggct ggtcaatcag aaatcatctt tataaacttc
    37141 gattgttttt gtaatgctgt atcattaagt tcattaaatc gtacagcaga taaatgttct
    37201 atcaaatttc gctcatttgc cgaggattca tcataataaa cgtaaaatta atgtatcctt
    37261 acatcgagta ataaacattt tttatacaaa aaaagacagg aacgtattta ctgggttaaa
    37321 tataatcatc ctgcttttca tcacaaaaac cgcagataat ccttcctttc cccggcagct
    37381 ggcgttatgg tcagatggtt tttgcaacaa atctcacaat aaaaagtttc aacatactga
    37441 ctatttaggg aaaaatatgc gcaagatcac acaggcaatc agtgccgttt gcttattgtt
    37501 cgctctaaac agttccgctg ttgccctggc ctcatctcct tcaccgctta accctgggac
    37561 taacgttgcc aggcttgctg aacaggcacc aattcattgg gtttcggtcg cacaaattga
    37621 aaatagcctc gcagggcgtc cgccaatggc ggtggggttt gatatcgatg acacggtact
    37681 tttttccagt ccggggttct ggcgcggcaa aaaaaccttc tcgccagaaa gcgaagatta
    37741 tctgaaaaat cctgtgttct gggaaaaaat gaataatggc tgggatgaat tcagcattcc
    37801 aaaagaggtc gctcgccagc tgattgatat gcatgtacgc cgcggtgacg cgatcttctt
    37861 tgtgactggt cgtagcccga cgaaaacaga aacggtttca aaaacgctgg cggataattt
    37921 tcatattcct gccaccaaca tgaatccggt aatcttt
//
LOCUS       DAJTOW010000028        37199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTOW010000028 DAJTOW010000000
VERSION     DAJTOW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.028"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..176)
                     /locus_tag="OO244_001550"
     CDS             complement(<1..176)
                     /locus_tag="OO244_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5663420.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO244_001551"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO244_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5663421.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO244_001552"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO244_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5663422.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO244_001553"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO244_001553"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5663423.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO244_001554"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO244_001554"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5663424.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO244_001555"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO244_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5663425.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO244_001556"
     CDS             complement(5180..5422)
                     /locus_tag="OO244_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5663426.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO244_001557"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO244_001557"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5663427.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO244_001558"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO244_001558"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5663428.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO244_001559"
     CDS             complement(8339..9313)
                     /locus_tag="OO244_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5663429.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO244_001560"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO244_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5663430.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO244_001561"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO244_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5663431.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO244_001562"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO244_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5663432.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO244_001563"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO244_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5663433.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO244_001564"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO244_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5663434.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO244_001565"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO244_001565"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5663435.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO244_001566"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO244_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5663436.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO244_001567"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO244_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5663437.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO244_001568"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO244_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5663438.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPLYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO244_001569"
     CDS             23180..23383
                     /locus_tag="OO244_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5663439.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO244_001570"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO244_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5663440.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO244_001571"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO244_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5663441.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO244_001572"
     CDS             26388..26660
                     /locus_tag="OO244_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5663442.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO244_001573"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO244_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5663443.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO244_001574"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO244_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5663444.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO244_001575"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO244_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5663445.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO244_001576"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO244_001576"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5663446.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO244_001577"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO244_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO244_001578"
     CDS             complement(31907..32305)
                     /locus_tag="OO244_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5663447.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO244_001579"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO244_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5663448.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OO244_001580"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OO244_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5663449.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OO244_001581"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OO244_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5663450.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OO244_001582"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OO244_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5663451.1"
                     /translation="MAVAANKRSVMTLFSGPADIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OO244_001583"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OO244_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5663452.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..>37199)
                     /gene="dcuC"
                     /locus_tag="OO244_001584"
     CDS             complement(36260..>37199)
                     /gene="dcuC"
                     /locus_tag="OO244_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5663453.1"
                     /translation="TLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGM
                     KIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYA
                     ILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQ
                     AFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVI
                     AICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVV
                     TSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8995 a         9295 c         9799 g         9110 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatacgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata aagcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgta tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctgctg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtg
//
LOCUS       DAJTOW010000029        35758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTOW010000029 DAJTOW010000000
VERSION     DAJTOW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.029"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..246
                     /locus_tag="OO244_001585"
     CDS             <1..246
                     /locus_tag="OO244_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5663454.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO244_001586"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO244_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5663455.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO244_001587"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO244_001587"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5663456.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="OO244_001588"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="OO244_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5663457.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="OO244_001589"
     CDS             complement(3257..3541)
                     /locus_tag="OO244_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663458.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO244_001590"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO244_001590"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5663459.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OO244_001591"
     CDS             complement(4442..5650)
                     /locus_tag="OO244_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5663460.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OO244_001592"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OO244_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5663461.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OO244_001593"
     CDS             complement(6445..8130)
                     /locus_tag="OO244_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5663462.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OO244_001594"
     CDS             8400..8777
                     /locus_tag="OO244_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5663463.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO244_001595"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO244_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5663464.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OO244_001596"
     CDS             9224..9511
                     /locus_tag="OO244_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5663465.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="OO244_001597"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="OO244_001597"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5663466.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="OO244_001598"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="OO244_001598"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5663467.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="OO244_001599"
     CDS             11268..11744
                     /locus_tag="OO244_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5663468.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="OO244_001600"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="OO244_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5663469.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="OO244_001601"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="OO244_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5663470.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="OO244_001602"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="OO244_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5663471.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="OO244_001603"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="OO244_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5663472.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="OO244_001604"
     CDS             16301..16789
                     /locus_tag="OO244_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5663473.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="OO244_001605"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="OO244_001605"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5663474.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO244_001606"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO244_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5663475.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO244_001607"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO244_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5663476.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO244_001608"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO244_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5663477.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO244_001609"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO244_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5663478.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO244_001610"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO244_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5663479.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="OO244_001611"
     CDS             complement(22241..22756)
                     /locus_tag="OO244_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5663480.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="OO244_001612"
     CDS             23405..25174
                     /locus_tag="OO244_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5663481.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSTQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="OO244_001613"
     CDS             25385..25708
                     /locus_tag="OO244_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5663482.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /gene="amiD"
                     /locus_tag="OO244_001614"
     CDS             25705..26535
                     /gene="amiD"
                     /locus_tag="OO244_001614"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5663483.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="OO244_001615"
     CDS             complement(26532..27545)
                     /locus_tag="OO244_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5663484.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..29074)
                     /locus_tag="OO244_001616"
     CDS             complement(27644..29074)
                     /locus_tag="OO244_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5663485.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO244_001617"
     CDS             complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO244_001617"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5663486.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="OO244_001618"
     CDS             complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="OO244_001618"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5663487.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="OO244_001619"
     CDS             complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="OO244_001619"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5663488.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="OO244_001620"
                     /gene_synonym="priS"
     CDS             complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="OO244_001620"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5663489.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="OO244_001621"
     CDS             complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="OO244_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5663490.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8912 a         8837 c         9192 g         8817 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atggtaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgtgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatt ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatacacatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcactc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28381 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28441 cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca
    28501 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28561 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28621 ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa
    28681 tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca
    28741 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28801 gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28861 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28921 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    28981 ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29041 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29101 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29161 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    29221 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29281 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29341 aaccgtgcga cgttattttc cagggcatac atcccggcgg cagccagaat cccggactga
    29401 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29461 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29521 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29581 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29641 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29701 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29761 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29821 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29881 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    29941 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30001 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30061 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30121 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata
    30181 attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga
    30241 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag
    30301 gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg
    30361 cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa
    30421 cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    30481 ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30541 ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc
    30601 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30661 ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg
    30721 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30781 tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct
    30841 ttatccagaa acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt
    30901 gatttgatat cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctgga
    30961 ttaatatcaa tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga
    31021 gtaccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg
    31081 attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt
    31141 agggcatgaa caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc
    31201 cccgcgcagc cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc
    31261 agtttgcgta actcttcttc ttccggcatc acgactggct gtggcgcatg ataccagtgc
    31321 gtggttgccc cttctggcgc aggttttaac gccacgtcgc ccggtaatac aacaacggaa
    31381 acgccacggt taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc
    31441 gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt
    31501 tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc
    31561 ggaacgttat tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg
    31621 cacgatccgg cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac
    31681 gccgccactt cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg
    31741 tcgctaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat
    31801 tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat
    31861 gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga
    31921 ggcactaacg gctaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg
    31981 agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg
    32041 gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta
    32101 cagcagccgc aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc
    32161 gcctccagta gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg
    32221 aatttcagac cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa
    32281 cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg
    32341 caggtcatca cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgagg
    32401 cgaccagcga taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta
    32461 cgccactcat cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca
    32521 tcggcctgtg gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg
    32581 cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc
    32641 atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag
    32701 ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg
    32761 gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat
    32821 tgcccggcgc gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc
    32881 gtttcttgcg taatgtgatg aacctgtatc cgccacgggc attgattcgt tggcatcgtc
    32941 atcgacaaac tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca gtggtgatag
    33001 aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg
    33061 cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa
    33121 tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc
    33181 ccagtttctc tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac
    33241 ctgcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat
    33301 atttaccaca ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg
    33361 tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca
    33421 gtttttcacg gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac
    33481 caaagcccac ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg
    33541 cctgggcgat gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa
    33601 cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt
    33661 tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc
    33721 cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag
    33781 gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt
    33841 tgtagagatc tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga
    33901 cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc
    33961 tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat
    34021 cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt
    34081 cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca
    34141 ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg
    34201 taaattctgc tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag
    34261 ccatcgggtt atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct
    34321 cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt
    34381 tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc
    34441 cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat
    34501 cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
    34561 tttctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
    34621 aagttatatt taatatacat gtttaagggt aagacgctta acgcggggat aaaagggatt
    34681 tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg aactaccgcc
    34741 agagaggtat tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa
    34801 accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag
    34861 ggtgaaatcc attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat
    34921 cagcccaggg atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc
    34981 gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc
    35041 gaaaccggag gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat
    35101 taaaccacca attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt
    35161 aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag
    35221 tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact
    35281 cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat
    35341 cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc
    35401 cagccacatc agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata
    35461 atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat
    35521 aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaatagctt ttaacgcagc
    35581 ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc
    35641 agaaaacatg aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac
    35701 tgaaagcgga agggcgagta aagttgcaat taaaaggaaa tgttatgcat cagagggg
//
LOCUS       DAJTOW010000030        35021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTOW010000030 DAJTOW010000000
VERSION     DAJTOW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.030"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..2319
                     /locus_tag="OO244_001622"
     CDS             <1..2319
                     /locus_tag="OO244_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5663491.1"
                     /translation="NDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIHQQDINV
                     LAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRR
                     YYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLL
                     SRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFT
                     KTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAF
                     ELGNNWTADLGRVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDAC
                     TTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYL
                     RQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTP
                     TMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTIN
                     SAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDL
                     LGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDS
                     SQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGL
                     NSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVT
                     YSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2316..3404
                     /locus_tag="OO244_001623"
     CDS             2316..3404
                     /locus_tag="OO244_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5663492.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3492..4790)
                     /gene="exuT"
                     /locus_tag="OO244_001624"
     CDS             complement(3492..4790)
                     /gene="exuT"
                     /locus_tag="OO244_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5663493.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5274..6686
                     /gene="uxaC"
                     /locus_tag="OO244_001625"
     CDS             5274..6686
                     /gene="uxaC"
                     /locus_tag="OO244_001625"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5663494.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMTKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6701..8188
                     /gene="uxaA"
                     /locus_tag="OO244_001626"
     CDS             6701..8188
                     /gene="uxaA"
                     /locus_tag="OO244_001626"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5663495.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8271..8822
                     /locus_tag="OO244_001627"
     CDS             8271..8822
                     /locus_tag="OO244_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5663496.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8827..10071)
                     /gene="sstT"
                     /locus_tag="OO244_001628"
     CDS             complement(8827..10071)
                     /gene="sstT"
                     /locus_tag="OO244_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5663497.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10667..11632)
                     /gene="alx"
                     /locus_tag="OO244_001629"
     CDS             complement(10667..11632)
                     /gene="alx"
                     /locus_tag="OO244_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5663498.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11903..12901)
                     /locus_tag="OO244_001630"
     CDS             complement(11903..12901)
                     /locus_tag="OO244_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5663499.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12980..13672)
                     /locus_tag="OO244_001631"
     CDS             complement(12980..13672)
                     /locus_tag="OO244_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5663500.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13749..14252)
                     /locus_tag="OO244_001632"
     CDS             complement(13749..14252)
                     /locus_tag="OO244_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5663501.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14337..15473
                     /gene="rlmG"
                     /locus_tag="OO244_001633"
     CDS             14337..15473
                     /gene="rlmG"
                     /locus_tag="OO244_001633"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5663502.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15758..16072
                     /gene="higB"
                     /locus_tag="OO244_001634"
     CDS             15758..16072
                     /gene="higB"
                     /locus_tag="OO244_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5663503.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16069..16485
                     /gene="higA"
                     /locus_tag="OO244_001635"
     CDS             16069..16485
                     /gene="higA"
                     /locus_tag="OO244_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5663504.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16530..18548)
                     /gene="fadH"
                     /locus_tag="OO244_001636"
     CDS             complement(16530..18548)
                     /gene="fadH"
                     /locus_tag="OO244_001636"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5663505.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18774..21125)
                     /gene="ygjK"
                     /locus_tag="OO244_001637"
     CDS             complement(18774..21125)
                     /gene="ygjK"
                     /locus_tag="OO244_001637"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5663506.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21142..21561)
                     /locus_tag="OO244_001638"
     CDS             complement(21142..21561)
                     /locus_tag="OO244_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663507.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21539..21892)
                     /locus_tag="OO244_001639"
     CDS             complement(21539..21892)
                     /locus_tag="OO244_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663508.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21893..22213)
                     /locus_tag="OO244_001640"
     CDS             complement(21893..22213)
                     /locus_tag="OO244_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663509.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22347..23780)
                     /locus_tag="OO244_001641"
     CDS             complement(22347..23780)
                     /locus_tag="OO244_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5663510.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23843..24292)
                     /locus_tag="OO244_001642"
     CDS             complement(23843..24292)
                     /locus_tag="OO244_001642"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5663511.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24289..27375)
                     /gene="ebgA"
                     /locus_tag="OO244_001643"
     CDS             complement(24289..27375)
                     /gene="ebgA"
                     /locus_tag="OO244_001643"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5663512.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27558..28541)
                     /gene="ebgR"
                     /locus_tag="OO244_001644"
     CDS             complement(27558..28541)
                     /gene="ebgR"
                     /locus_tag="OO244_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5663513.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28759..29090
                     /locus_tag="OO244_001645"
                     /pseudo
     CDS             28759..29090
                     /locus_tag="OO244_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29132..30511)
                     /gene="ygjG"
                     /locus_tag="OO244_001646"
     CDS             complement(29132..30511)
                     /gene="ygjG"
                     /locus_tag="OO244_001646"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5663514.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30929..32449
                     /gene="aer"
                     /locus_tag="OO244_001647"
                     /pseudo
     CDS             30929..32449
                     /gene="aer"
                     /locus_tag="OO244_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32603..33226)
                     /gene="nfeR"
                     /locus_tag="OO244_001648"
                     /gene_synonym="yqjI"
     CDS             complement(32603..33226)
                     /gene="nfeR"
                     /locus_tag="OO244_001648"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5663515.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33503..34248
                     /gene="nfeF"
                     /locus_tag="OO244_001649"
                     /pseudo
     CDS             33503..34248
                     /gene="nfeF"
                     /locus_tag="OO244_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34302..34377)
                     /locus_tag="OO244_001650"
     tRNA            complement(34302..34377)
                     /locus_tag="OO244_001650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34342..34344),aa:Ile,seq:cat)
     gene            34502..35008
                     /gene="mug"
                     /locus_tag="OO244_001651"
                     /pseudo
     CDS             34502..35008
                     /gene="mug"
                     /locus_tag="OO244_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8564 a         8991 c         9190 g         8276 t
ORIGIN      
        1 aatgatgaag aaagttcggc aacgttgttt attaatccgc aatggaattc agctttcgat
       61 gcgaagtcat tatatttaaa tccagacaaa aatactgtta acgcttttat acatcagcaa
      121 gacatcaatg ttctggcaca ggatgattac caatcgttgt ctattcaggg aaacggtgcg
      181 ctgggaataa cagaaaatag ctatattggt gcacactgga atttcaacgg ttatgatgca
      241 gatgatgtca gtgacagtaa tactgacgtc agcgatctct attatcgtta tgatttttta
      301 cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca cactatttaa tgcacaaggc
      361 gggaacttta cctttaactt tctgccactc ggtgcaatcg atgggatgcg tatcgggtcg
      421 acactgagct atttaaacca ggcgcaaagc cagcaaggaa ccccagtaat ggttctgctt
      481 tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac ttttgggatc gttttatctc
      541 aatagtggtt cgcaatttat tgataccagt tcctttccgc caggcagcta cagcgtggca
      601 ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc ttgtgccgtt taccaaaacc
      661 ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg caggtaaaac tacatcacag
      721 gtttctgatg atgaaagttc agcttatcag ctaggagtac gcctgccatt acatccgcaa
      781 tatgagctct acgcagggct ggcgaatgcc gatgatgtga gtgctttcga gttaggtaat
      841 aactggacgg cagatttagg cagggtgggg aatcttgcaa tcagcgccag cgtgttccgt
      901 aacgatgacg gcggcaaagg tgatatgcaa caggccaact ggagtaatcc gggatggccg
      961 acgttgggct tttatcggac caactctgac ggcgatgctt gtacaaccga cagcagagag
     1021 agctataacg ccttaagctg ttatgaaagt atttccgcga cagtttcact gaattttgtc
     1081 ggctggaata tgatgctggg ttatacctgc acacaaaata acactgatga tagtttgcgt
     1141 tgggataaac agcagagctt tgaaaataac tatcttcgcc agacaacagc gcaaagtatc
     1201 tctgaaactg tacaacttag cgcttcccgc gctattgtga tgcgtgactg gattttgagt
     1261 acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg ataacgacga caacggcttg
     1321 tacttatcgt tttcgttatc tgacacgcca acgatggata gcaataacaa cagccattca
     1381 accaatgtgc ctacggatta tcgttatagc gaacaggatg gcgatcaaac gtcatggcag
     1441 ttatcgcata ccttttataa cgattcattc agccataaag aacttggcgt aaccgtcgga
     1501 ggcctgaaca ccgataccat aaacagcgcg gttaacgggc gttgggatgg gcaatacgga
     1561 aatgtctacg ctaccgtatc tgatagttat gaccgtaaga atcatgatca tctttcggcc
     1621 tttacgggca cttatagctc tacgctggct gtcagtcgct atggcgttaa tttgggagcc
     1681 agtggtacag acgatttgct gggtgcggta ctggtggatg tgaaaggctt ctctgaacag
     1741 gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg caggcagccg aactttgcag
     1801 cttggtcaaa gtgacagtgt gttgttccct tatcctggat ttcagtctgg ttttgttgag
     1861 gttaacgaca gtagccaggg caatcagcag gggacaacaa acatcattaa cggtgcaggg
     1921 aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc gcgaagtgtc tgccagcttt
     1981 aactacaact atatcggtcg tttattactg ccggcagcgg tgaaaaaatt cccgattgtg
     2041 gggctgaaca gcgccatgtt actggtagct gaagatggcg gatttacact tgaaattaac
     2101 ggcagcgaaa aagagctcta tctgctttcc gggcagcaat tccttaagtg tccactgagt
     2161 gttgtaaaga aacgcgccag cattcgttac agcggagatg ttacttgtag tgtggtgact
     2221 tattcacaat taccggagtc gattcaggtt caggcacagt tgaaacagcc taaattacgt
     2281 ggaaacgttc agacggcgca aagggaggtt gcaccatgag aaatcgattg attgcggcga
     2341 tattgggctt gtttggcacg ctcactggcg ttcaggcagc tcctgacgtg accagtgaaa
     2401 ttacgtatga tttggcatct ggtagagcgg attattactt ctggaaggat gaagcgtcgg
     2461 caggaaataa tggatatatg tggtatgaat gttcgtatcc tgacctccaa caaacctgta
     2521 cagctaatgg aaatatatcg acagtacaaa tctatttaac tgaacaacgc agtgggatgc
     2581 gttggccggt aaaactcaaa ggatttaaaa cagccattgt aagtagcgat gaagcgccgc
     2641 taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa ggattctaat agatcttcat
     2701 gtacagaaga tggtcaacat tattatatat acgatacaaa gtttcttacg ctctaccttg
     2761 agcagacaga gatgaagaat ttgccgattg gtggcgtctg gaaggggaaa gttaaattac
     2821 attcgaacag cccggcccag gactatttcg caaatattac cctgaatacg ctcgacccca
     2881 accatattga cgtgttcttc ccggagttcg cccacgccac gccaagggtg cagttagact
     2941 tgcatccaac aggaagcgtt aacggcagca actacgcgca agatctgacc atgttggaca
     3001 tgtgcctgta cgatggtttt aacggtaatg ccatcagtta tgaaatcatg ctcaaagatg
     3061 aagggcgacc agctgcaggg cgcagagacg gttacttctc tatctatcgt cagggaggga
     3121 ccaccaccga cgagggagaa cgcattgatt accgggtcaa aatgtacaac ccggaaaccg
     3181 gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg gaacagcatt aacctgaaac
     3241 gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt gatgtgtgtg ccgacaccct
     3301 tgactctggc agtcgataaa ttcagcgtga tggataaaca ggccgggtac tacatgggca
     3361 aattgtcagt aatctttacg ccttccttgc caaccatcaa ttaaccctgc aaaccggaca
     3421 aacgcttttt tctatataaa cgacgaagcc gcctgataag gcggcttcgt cgttataact
     3481 tacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     3541 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     3601 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     3661 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     3721 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     3781 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     3841 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     3901 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     3961 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4021 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgcaagg
     4081 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4141 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4201 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4261 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4321 gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtag
     4381 cctactgcga tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc
     4441 gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag caacagctag gccaccccag
     4501 ctacctgcca gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa
     4561 ccgattttcg tacccagcac atccagtaca taacctgcta ccggttgcat gaccgtataa
     4621 gcagcagaat aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc
     4681 atcagagttg gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg
     4741 agcgtcacca gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc
     4801 atcgttttgt tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta
     4861 gttacgaccg ggtaaccgaa aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     4921 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     4981 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacctt
     5041 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5101 aggccagtac tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact
     5161 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     5221 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     5281 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     5341 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     5401 aagactatcg ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc
     5461 gcgctatgcg taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg
     5521 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     5581 ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg caaattgctt tctccgtcaa
     5641 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     5701 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     5761 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     5821 gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac ctttaacgac tacatgacga
     5881 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     5941 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     6001 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     6061 ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     6121 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     6181 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     6241 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     6301 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     6361 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     6421 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     6481 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     6541 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     6601 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     6661 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     6721 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     6781 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     6841 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     6901 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     6961 ggatcagtat cgctatcaac ttgattttca ggatctgcct gcgcaagcgg cagatcctga
     7021 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct
     7081 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     7141 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct atggctgctc
     7201 acaactgggc gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc
     7261 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     7321 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     7381 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     7441 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     7501 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     7561 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     7621 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     7681 cttcaaacag tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa
     7741 caaagcgggc ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg
     7801 ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa
     7861 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     7921 ccatatggtg ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt
     7981 gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     8041 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     8101 cgttgagttt gccaacggta agcaaacctg caacgagcgt aacgacttcc gcgaactggc
     8161 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     8221 ttcataatgt gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt
     8281 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
     8341 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
     8401 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
     8461 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
     8521 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
     8581 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
     8641 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga
     8701 ttgagggtag ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga
     8761 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt
     8821 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
     8881 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
     8941 gccgataata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
     9001 attacaggcc agtgggatca gcagcaaaga accccccgcc acgccggatg cgccacaggc
     9061 acacagagaa gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag
     9121 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat
     9181 ggtggctccc agcggaatag aaactgaata ggtatcgcga tccagattca gcttttcaca
     9241 cagcgccata ttcaccggaa tgttcgctgc agagctgcgg gtgaagaagg catacacacc
     9301 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
     9361 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
     9421 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc
     9481 aaaaataccg aacggtgcga agcgaatgac cagtttcacc ataaaggtaa cggcattcga
     9541 catatcattg accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc
     9601 gatcgcccac accagaatcc cgatgtagtt acctttcagc agggcgtcga tggggttgga
     9661 aaccatgctc attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc
     9721 acccgcgcta ctggacaagt gcagggtaga agggaaggca aagctgaaga ctactgcggc
     9781 cagagctgca gagaaggtgc ccagcagata gaggaacaaa ataggacgga tattggtttt
     9841 ctgcccgtgc tggtggttag caatagatgc cattaccagc atcaacacca ggatgggggc
     9901 aacagctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
     9961 ttttgagatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    10021 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    10081 ctttcattgt gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    10141 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    10201 gattttaaag agaggtcatg ttttgcaact taatggttgc atttatttaa cataaacaga
    10261 gggaaggact ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg
    10321 agaacggttg cgctccttta acgtgttatg ccatataaag tcactggcgc agggagcgcg
    10381 cagggggcgg ccaatcgccg ccgccccctg cacccccagg ctctggcgaa caaaatcgcc
    10441 gctgcgcggt tccctcagcc tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc
    10501 ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt cggcgatttt gacgccacca
    10561 acacccgtgc ggtctttttg taaagagcta taagccgatt acataattaa tagctcacct
    10621 ttgtctggaa tgagttatct ctaggaatgg cttaatatac agcgaatcat tctccccgct
    10681 gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc agaatgccaa
    10741 acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc agcatcttga
    10801 taccgataaa caccagaatc accgccaggc catatttgag catcgagaaa cgctctgcta
    10861 cgcccgccag caggaaatac atcgcacgca ggccgaggat tgcaaacagg tttgaggtca
    10921 gcacaatgaa cgggtcagtg gtcacggcga agatagccgg aatgctatcc acggcaaaaa
    10981 tcacgtcgct caattccacc agaatcagta ccagcatcag cggtgtggcg tacagcaatc
    11041 cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc
    11101 gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca
    11161 tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag tcgaactgcg
    11221 aaatcagcca gctgcctgtg aagatcatga tggtacgcag aacaatcgcc ccgagcacgc
    11281 catacaccag cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg aacaacatca
    11341 gccagacaaa gacgttatcg accgccagcg atttctcaat cagataaccc gtgagaaagg
    11401 ccagggcctg tggatcggca acggcgcgac cttcggtttg caccagatac caccagaaag
    11461 cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca gcctgtttca
    11521 tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc agcataatgg
    11581 cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc atagaagttc
    11641 cttacacata aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga
    11701 cctcgccttc cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt
    11761 cgggttgccc ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat
    11821 gaagttactc ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct
    11881 gttacatacc ttttacactg ttttactttg gcaagtatag ttttacgcta tctgccggaa
    11941 aaatcacccc agtttggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg
    12001 ccagccctgg atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt
    12061 agagcatggt attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca
    12121 caaagcagac tttctggcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct
    12181 gaatctcgct tcccaggaca gaatcactga cctttgagtg ctgcaaggtg acgctgaaat
    12241 caccgtaatc catcaccacc acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg
    12301 ttgcctgcac gcttttcggc tcaccaaata acgccaccgc cgacgccagg cagtaaaagc
    12361 cgatatccat aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt
    12421 ccaggtaacg ttgataccgc gaggaatact ggcaatagtt gaaaaagact ttacgcagtt
    12481 tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa
    12541 atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc aatggcggca tccacttccg
    12601 ccagattcga cgccagcggt ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt
    12661 gtgtctggga aaagtgcagg gaattcgggc tggcaatata caccgcgtca atggcatcgc
    12721 tttccgccat cgcttccagc gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt
    12781 gctgagcctg ctcaaggctg cgggaatata cggcggttaa cttgtattta ccgctctcat
    12841 gggcggcctc gacgaactgg cgagtgatcc agttcgtacc aatcacagcg aaacgtatca
    12901 taacgcgtgg atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt
    12961 cgacgtgcgc agatacgcat tattctttcg ccggacagcc atgctcacta aacggcccaa
    13021 tcatgaccga tggcccgaga tatttcggct ggcgatggag gatttttgcg tcaatcaccg
    13081 cgctgacacg agcgagtttc tcccgcagtt gagtataaaa accgtgcttc atattgaggt
    13141 ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt tgccagccag atggtgcgtt
    13201 ggttatgaaa ttcttgcgag ataatggtga tatggttttc cccaaacacc tttttggcac
    13261 gcactaccga atccagcgtt gagaatccgg catagtcgca aaagatcact ttcgccggta
    13321 cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt
    13381 tatcgccact caccagtagc catttcactt ttccggcgtg gtacaacgct gctgcggtat
    13441 caatacgtcg ggtaaagtag cgattacccg gcctcgcccc cagcaataaa cccacgttac
    13501 gcgccgggac ggcgttgaca tcgccccagg tcagctgctt gctggcgttg accataacgc
    13561 gatcggcgat aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag
    13621 tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta
    13681 tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga
    13741 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    13801 aactcaagct ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta
    13861 tgctctttct ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc
    13921 ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgctgccc
    13981 tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg
    14041 tcatacatca ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga
    14101 gtatattgcc agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
    14161 agcacatcgc ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg
    14221 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    14281 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    14341 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    14401 ttaacccgct acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca
    14461 cagaaatccc cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc
    14521 tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg
    14581 agaatttacg cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    14641 actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg
    14701 aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg
    14761 ccaaagcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga
    14821 ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc
    14881 cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc
    14941 ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc
    15001 aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta
    15061 ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc
    15121 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc
    15181 gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg
    15241 tgctctgcaa ccctccgttt caccaacaac atgcgctgac cgataacgtc gcctgggaga
    15301 tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc
    15361 gccatctgga ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga
    15421 cgaataataa atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg
    15481 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttaccccc
    15541 aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc
    15601 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt
    15661 ctacgtttat tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt
    15721 tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc
    15781 attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa
    15841 cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct
    15901 ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg
    15961 tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca
    16021 taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt
    16081 gccgacatct tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt
    16141 cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat
    16201 cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg
    16261 cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat agccgtgatt
    16321 cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa
    16381 tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa
    16441 ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccgaat
    16501 gcgttgcatc cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg
    16561 gcaattgccc gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca
    16621 gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag
    16681 atcaccacat tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg
    16741 tcatcgtcaa tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg
    16801 gtggtgcgat ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg
    16861 cgctggagca tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag
    16921 ccaccagcct gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc
    16981 tggctggtgg attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca
    17041 caaccgatga tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa
    17101 ctcaatacct tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg
    17161 gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc
    17221 agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc
    17281 tctttgccgg ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca
    17341 aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt
    17401 ccagcgccga ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt
    17461 tcgtggcagg cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg
    17521 aggcagccct ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct
    17581 tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg
    17641 ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc
    17701 ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg
    17761 ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc
    17821 gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct
    17881 tcgactaggt cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg
    17941 cgcaccgtac gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac
    18001 tggtcactac gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag
    18061 cccatcactt cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa
    18121 tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg
    18181 ggggcctgca atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc
    18241 aaaatttgca gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg
    18301 tgtgggatct ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctgttaaa
    18361 tctggtgcaa tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa
    18421 aacgcagcca gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc
    18481 atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg
    18541 tagctcataa aattgtccag tatgtaaaat aattgttatg tagtcggatg agttctaatt
    18601 tagccgtcga tggagaaaag ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag
    18661 aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg ttttatccgg cttgggggat
    18721 tgcacatgta gggcggataa ggcgttcacg ccgcatccgc cagccgttgc aaattactgc
    18781 ttacggaaaa aatcgttata caacatatac aaatgagcgg cactccagga gaaatttggt
    18841 gcgccttgct gcgcgcctgt cagcgggttg taattctcct gaattgggcc atcggcggtt
    18901 aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca gggcatcatc acgataaccg
    18961 taacgctcca tccctttcag accaaaccag aactgatcca cccatacgcg cccgcgccag
    19021 tagatatcag cgccaaaagc cggattggtt aacgccgccg ttcccagcgg aacaaaggtg
    19081 ttgaactctt taggatcgag catcaccttc accaccgcgt cggcattggc ctgcgttgcc
    19141 gcaccgttaa acagcggcga ccagccttcc ggccctttac cgcgctcaac aatcggtttg
    19201 cccgcgcagc cgttcgccag tggtttatct tcaatacgta cgtcatagta gaactgggta
    19261 gtcgggtcga acatacaggt gttgatgtag tccgcgagct gctgtgccaa ctggcgatag
    19321 cgtttggcct cttctggctt accgaggatg gttgccatct ccgccagata atggttatcg
    19381 ctgtacatat agctggcctg atccaccgac tcctgcaata gcgagtagcc cagcaacgtt
    19441 ccgtcctgac tgcggttttc ggcgaatttc accgtccagt cgctacgttt gccgccatta
    19501 gcgacatatt tatccaactg ttctttgtcg ataaaaccaa agacggcggc atcatcacgc
    19561 cccgattccc acgacgcagc gacctgtgcc ggaatttcca gactgtcgta ctggcctttc
    19621 tccaccacgc gggcgtagtt gttcagccca gactgcgtct cttctttatc gccttttttc
    19681 accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt tgtcgcgggt cgcgccatat
    19741 tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc accagtcgtg ataggccacc
    19801 agtttcgggt acatctctgc caaccaggct ttatcctggg tgacgttgta tacttccatc
    19861 accgaccagg cggcaaggct gggtttggta ttacgttcgt tccagttgcc gccatcgcca
    19921 ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga caaagcccac atcctgcgga
    19981 cgcacgctgt cgccaggctg gatctgccag gagaagaccg cgcggatatt ctctttggcg
    20041 atatccggat tgaaatgcgc catcgcaaac gcctgcttcc aggtatccca cggccaggtc
    20101 tgattgccgg agaaccagcg tccggtcacc gacggcgtga cggtgttata attcaccgca
    20161 ccgcccggtg agcgccagtt accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc
    20221 tgttccggcg tcgcatccgg attggttaac cctttcttca ggtattcttc ccagcgttgc
    20281 tgcgaggcgg tgagataaaa cgccggacgc gccagaatat cgcggatctg catttgctct
    20341 ttgctaactt cctgagcggt cagcagatgg gaataggtgg tgtagagcgt ggtcgaaccg
    20401 ttgatatgcg ccttactggt aaagcgatta ccgttgattt cagtctgcac cggcagggat
    20461 ttatgcacct gatattccga ttcgccggag gtcagcagat cccaggtggc gcgcactttg
    20521 ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt tgcgctgata gtcagggtat
    20581 tcgccagcaa tggttttatc ggaaagcggt ttcccttctt tcgcttccag tttttccagc
    20641 agttcgccat cccacaccag atccagcggt ttattgctga tgattttagt ttccagtagt
    20701 gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct gcacatcttt tgctgtcagt
    20761 ttttgcacca gcgcgccggg aatactgtat gcctccagcg tgaagttgac tttcttgccg
    20821 tcttgccaga cagtcaggcg gtcgaaattg ctggccataa agttgatgta ttcttccgtc
    20881 agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc cgtctggcaa cagatgacca
    20941 tgccaggctc cgagatcgaa aaacggatta aaacgctggt gatcgtcgta atcgtaatcc
    21001 ttcatgtact gcggcgcacc agtacggtta atcacgtttt tataattgtc ggcgttagcg
    21061 gcatgagcgg aaaaacttat cagcagagcg caggttactg gcgttaaaat agttttgatt
    21121 ttcatgagat tacagtcctt attaccagta gaaatattgc atcaggaaga tttggtcgct
    21181 gggatcgtta gaggcatttt tcatgtagaa gcggctatca aaagccgtgg cgaaacgtcc
    21241 gccgccatat tcgtaccaga taccaaactg catgaaagag gtggtttcat cgtcagcgtg
    21301 gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga taaacgcctt ttaacgcttc
    21361 atacatggcg ctcaccatca tcccgctttc acttcccggt acgtccagcc cgtcggcacg
    21421 tccggtatgg tgccaggcgc gagcggcaaa gttaggggta aacaggcccg tgagatagag
    21481 ttgtcctgtg ccgtcggtaa tttccagacc attcatccag gtgatgcctt cggcaaggtc
    21541 atactggatg taaccgttca tccattgccg gataggtgta tttgtcgtcg tagcgatcgt
    21601 acttgccaaa atgtagccag gctttgcttt cgtcatgatg tccggcaggt gtggcggtga
    21661 cgctataacg aaagttgccg cttaagttct gtaccttcag cgcggtcata atgtcgcggg
    21721 tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc ccaccattgc aggtcatcca
    21781 gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc atcattggta cgataaccgc
    21841 catagaaacg gttaatccca ccttcgaaac caccccagct atggtcaggc acctaggcat
    21901 taccgtcgtg gtcggcctgc acggtccagc cttcgccgta aatcaggccg aaccaggagc
    21961 cgtacttcat ctccaggcca ccttcaatat aggtgccgtc gctgtattta tgtttattct
    22021 caccctctaa atccatatat ccaccaagcc ctaattcgcc gtaaaaacga aatgaaggag
    22081 aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg ttctgccgga cgtgcttctt
    22141 ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag taatgctctg catggtgcag
    22201 tgattaattt cattgtgtta atccatgagc taaagaaaaa gtacagcctt ttatcgttgt
    22261 aattataaaa atataaaggc agaaactatt ttccctctcc tcatccagga agagggaaaa
    22321 tgcctgctca ttttttatta gtctgcttaa tcaacattgc tggctggcgt cgcttcaata
    22381 tggcgctctt cagccgttaa tccctttata tatttgctgt atttccacca cgcaaagccg
    22441 aggaagataa caataccgcc gacgttataa aaaatgatgg tcagaatatt cgcgccagtc
    22501 gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa tcagcattga aacaacgata
    22561 ataccggtgc ggcgggagcc catacggaaa tcgcgtggca ggtgatctaa tttggcgcgt
    22621 aaattcaggt aagccagcat gatgaataac ggcggaagca tggacgctgc ggcggtcata
    22681 ttaataatgg tattcatcag atcctgcacg gtattggaac cgagcatcgg gataatcatc
    22741 agcgggatga cgatcagaaa ctggatccat gctgcgcgcg ccggaacgcc gttttcgtta
    22801 agttcgacgg ttttcttacc aaagatgcct tccgggattt cggagaagaa aattttcacc
    22861 ggcgttgcgg tccacatcag cagggaaccg aacattgcgg taaaggacac cagaccgaca
    22921 aagcgattca tcaacgcttc cggcagacca aaatacgccg ccatgccgtg gaatacctgc
    22981 accgatccgc cggtaaattt caactctttg ctgctgacga agacgttaat cagcaccgag
    23041 gagacggaat acagtacgcc gataaaaatc ccggcgagga tgatcacttt aacgaacgat
    23101 ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg actccgcgcc acctgcggcc
    23161 ataaagatcc aggtggtaac gccgaggaac gcccagttga agttcgggat catcgcgtca
    23221 acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg ccgtacctgc cagtaaaatg
    23281 taggagagcg ttaacagcag catcagcgtt gaagtgacgg aggtaattgg ccccagcatt
    23341 ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca gcaccatact gataacggtg
    23401 gtggcaaccg gcgtcataat atattcgtag ccgaggaagg catacgaagc ataagcaata
    23461 acgcgcggca acagcgaggt gaaaaagaac agatttacga accagtaagt gtaggcagta
    23521 ataaatgccc aacgtccgcc cagcgaactt tttacccacg cgtagacacc ggcttctgag
    23581 tttttattta acgaaacaaa ttctgcgatg atcagacaga agggaataaa ataaaaaatc
    23641 gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct caatattatt attgataacg
    23701 ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca agccgaattt gccgattgta
    23761 ttacgtttgg tatcagacat aacacatctc cgaaaatgtg tagggtagcg gtggcggcaa
    23821 actccttttg ccgccgtagt tgttatttgt tatggaaata accatcttcg atggtgactt
    23881 tgagaaccac ttttttgacc gcgttattgc agataaaccg atacgcttca tggatatcgc
    23941 aaataacgat ttgcccttcg tggacctcta cggtttctcc gcagcctttt aaatattcac
    24001 ggtcagtttc atcacgataa tattccacta cctgtaatgt ctctttcggc gcatattcaa
    24061 ttttttgctg cccttgcagg tagtaatgca cttcaaaata gcgtcgatgc ccggtaaata
    24121 gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa tgaatcaccg atggagtggg
    24181 cgacgccagg ctgaatgttg tcgatatttt caatcgcttc aacgcagcgt tgccacttct
    24241 tgccagaggc gtaaatctgg cggaactgtt ctaagttatc gatgatcctc attgcttatt
    24301 ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac tcatacgacg ccaggctttg
    24361 cgcggtagct tctccgccag aaaccggcag caacgtaaag ccgtagctga agtcacggaa
    24421 ccagacgcgc caggagtcca gcacctcgct gccccaggag ttggagccga ggccaagtag
    24481 ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc agctcgttac agtgctgggc
    24541 agcgtggatg ttttcctggg tatagtgcca ggcgctgaag ttaattgggc gctgcggaac
    24601 caccagcaga ccgttgccgt ggcggttagt cagtgccgtc cagcggacat gctgacggtt
    24661 gccgttgttc tgcgggaagg gatagttctc gaacatggca tcgacggtgc tgcgccagat
    24721 atcgatgatg ttagcctgct ggctgtcggc gtagttttcg cccggtccac gaccgtaata
    24781 cgccacctga tcgtattcgc cgttaatccc catggtgaaa ccgatgcacg gaatgatgtg
    24841 cggatagtcg ccgtaacgct cgccggaaag cgccacgtta acctggccat cggcagcgat
    24901 gcgccagatg taggtacagc gcatcccgaa gtcaaacacc ggcggtgcaa taactgtgcg
    24961 gctgatgatc aacacttcac catcgctctg ttctacggca aagtcgcgca gatgttcctg
    25021 catgatctgc aaatgattcg gctgccacaa cccttcgtac tcctgcttgt ggttgtcgat
    25081 catcggcttg aagaagttga tctttggctc gcgggtcagc agcgactcgc catttacctg
    25141 ccaggatgtc ggtttgccac tcatttttga gaaggtgatc gcgaagttgt agccgcgaac
    25201 ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc gcattatttg gtgcgaaagg
    25261 cactggctgc gcggtgtttt ccttcagcgg gaactgataa gtggcgatag aatgcccggc
    25321 ttcgctgtag cgggtgcggg aatctttggt caccgtaatg ttgaggaacg cttcgcgggc
    25381 gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg ttcggcgcaa cgtcgcgcag
    25441 tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc acctctgcgt gcagggtgta
    25501 gtcatcaagc gtggtaaacc acagtttatt ttcgactttc aactcgccgc gagtcagcgc
    25561 gtggattttt accggcgcga taacctgttt gtactctttc aggcccgatc ccggcgtctg
    25621 atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt cgccgtagtc
    25681 gccgccgaat ttataccaga cattgccgtg gtcgtcctgt gcctggatcc cgtggtcgca
    25741 ccactcccag acataatgac cctgaatgca atcgtgctta tagaagacgt tctggtactc
    25801 cgtcagcccg cccggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt
    25861 cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat
    25921 aatatcgacc acttcagcat cgcgatcttc ttcgtaatga accagtcgcg tgtcatccag
    25981 cgctttcgcc gcatggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag
    26041 cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg atatggcgaa caatgcgctc
    26101 gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc
    26161 aaagccgtgc gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag
    26221 ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg
    26281 cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg
    26341 atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag
    26401 accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt
    26461 gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca
    26521 ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa
    26581 atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt
    26641 cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc
    26701 gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt
    26761 tccgaccaga taaacatcgc ggaagatccc cgccgaccac cacatatcct ggtcttccac
    26821 gtaggtagag tccgcccact gcatcacgcg cacacacaac aggttgtcgc cggttttaac
    26881 catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac ccacatactg
    26941 accgttaacg tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt
    27001 accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct
    27061 ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc
    27121 gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc
    27181 agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgact
    27241 gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga
    27301 atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt
    27361 ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt
    27421 agtttgtgta attgctgggg gtttagtaaa atttttacta aaaagcagca ggtaaatgca
    27481 tttactttaa ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa tgattgccga
    27541 acagtgtgta aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag
    27601 actagcagcg gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga
    27661 cttcccatca tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg
    27721 gtggggatat cgttaacgct gataagcgaa atatcttgtg ggatgttcag gccacgttca
    27781 tgaattgccc gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga
    27841 tagtcttccc gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg
    27901 ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg
    27961 acctcacgaa tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta
    28021 acgccctggt tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc
    28081 accgcatcgt aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac
    28141 gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg
    28201 tttttaatgt ctggtaatcc gttgtgttca taacagttgg tcagttcgat cgccagcttt
    28261 tcgcactgag tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc
    28321 tcctgctggt agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt
    28381 ttacgggcgc tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc
    28441 gtctcttctt tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg
    28501 gatacgccag cttcgattgc gatgtctttt aatgttgcca tgtttacctt ttttactttc
    28561 cctgatgtgc gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata
    28621 aagcgcgttt ctttgacgct gtaacggtaa cacagcggct taggaatgtg atcacgcctg
    28681 cattctccat ttttagccta caggattgag aaaaactttt ctatagttta gtaaaatttt
    28741 actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg attttgcacg tctggaaatg
    28801 cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg ccgacaagct gtacatcgta
    28861 caggttgatg tggggaaaaa acactgcaaa ccgtgaccag cctggtgccg tactacagcg
    28921 aagaggagct gatggggaaa acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc
    28981 gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg
    29041 tgttgttaac gccggagcgg atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga
    29101 tggcgacgtc gtatcgccat ccgatttgat attacgcttc ttcgacactt actcgcatgg
    29161 ccgccagcgc cttacgcgcc gctttgatca ccagttcaca ctgttcaatg gtcagtgtca
    29221 gtggcggttc aatgcggatc gttttggcgt tattgagcgt tccggccacc agtacgcgct
    29281 ggcggaacat ctcgctggca aagttatagc cgatttcgtt atcaacaaac tcaatcgcca
    29341 tcaacatccc tttaccacgc gcttcctgta ccagatcggg atattcccgc gccagttgac
    29401 ggaaaccgtc cagcaacata tcgccttttt gctcagcctg agccggtaag ttctgctcca
    29461 gcaacacatt gatggtcgcc agcgccgccg cacaggccag cgggttgccg ccaaaggtgg
    29521 tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg
    29581 cgccaatcgg catcacgccg ccgccgaggg cttttgccag acacagaatg tccggctgaa
    29641 cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat
    29701 cgaggatcat cagtgcgccg aactcatcgc acagcttacg cacggcggtg agatagcccg
    29761 gcggcggcag aattacgcca ccttcgccct gaatcggttc gaggatcacc gcagccacat
    29821 catcaccggt ttttttgcac tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg
    29881 gcacatgacg gaaccctggc agcagtggca taaacggttt gcggaaggtc gatttcgccg
    29941 tggccgacag cgcgccaagt gatttaccgt ggaacacgcc gctggtggca ataaaagtaa
    30001 atttgccgcg cggcgactga taagccttcg ccagcttcag cgccgcttca acggactcgg
    30061 tgccgctatt acagaagaag ctgtatttca gtttaccggg cgttagcgca gcaagggttt
    30121 tcgccagcat agcccgtaac ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa
    30181 gttgattctg tacggcggaa accacaactg gattacggtg ccccacgttg aaaattccaa
    30241 aacctcccag gcagtcgata aactcctgtc cctgggtgtc gacaagcgta tttaaacctc
    30301 ccgcttgcca ctctacggct ccgtaatccc cgccggcggt aacagatttg cgatactcta
    30361 aaaaccccgg attgacatgc tctttgaagt attcaatcac ctctcggtta agtgctttca
    30421 tctcctcatg atccagcgtt cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta
    30481 aagccgatgc actcgaaggt aacctgttca aaatatgctc cggaggctcg cgtatcacat
    30541 gataccgatt taagtattgc agggattgcg ccactccggc tgcgatcgcg aaaatcggga
    30601 taaacacaaa tgcggttatc atttgtgcaa tatttaatgc tatccgttaa catttgttgt
    30661 tattttgcac ggtttttgtc gggtatggat ttgatgattg ctgcgcaaat gcacgttttc
    30721 agggcaataa ttgcctgatt tttgggcaag ataattaacg ttttcgtata gtaacagagt
    30781 gaaagtattt taaatcaaag aattagattt gtgctttaat cgcaaattgc gatctaaatc
    30841 aaattaatcg gttaaagata accgcagcgg ggccgacata aactctgaca agaagttaac
    30901 aaccatataa cctgcacagg acgcgaacat gtcttctcat ccgtatgtca cccagcaaaa
    30961 taccccgctg gcggacgata ccactctgat gtccactacc gatctgcaaa gctatatcac
    31021 tcatgctaat gacacttttg tgcaggtgag cggctttacc ttgcaagagt tacaagggca
    31081 gccgcacaac atggtgcgtc acccggatat gccaaaagcg gcgtttgcgg atatgtggtt
    31141 caccctgaaa aaaggggagc cctggagcgg catcgtgaaa aatcgccgca aaaatggcga
    31201 ccattattgg gtgcgggcca atgcggtacc gatggtgcgc gagggaaaaa tcagtggcta
    31261 tatgtcgatt cgtacccggg cgacggatga agagattgcg gcggtggagc cgctgtacaa
    31321 agcgttgaac tccggacgta ccagtaagcg tattcataaa ggcctggtgg tgcgtaaagg
    31381 ctggctgggt aaactgcctt cattaccgct tcgctggcgg acgcgtggag tgatgaccct
    31441 gatgtttatc ttgctggcgg ccatgctttg gtttgttgct gccccggtgg tgacgtattt
    31501 cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt gaatggcaga ttgtgcgccc
    31561 gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc ggagagcgta atagtgttga
    31621 gcacctgaat cgcagcgatg agctggggct gacattacgc gcggtagggc agcttggcct
    31681 gatgtgccgt tggctaatta acgatgtctc aagccaggtg tccagtgtca gaaatggcag
    31741 tgagacgctg gcgaaaggca ccgatgaact gaacgaacat acccagcaga cagttgataa
    31801 cgttcagcaa acggtggcga ccatgaacca aatggcggcg tcggtgaaac agaactctgc
    31861 cacggcgtcg gctgccgata aactttctat caccgccagt aatgcggcag tgcagggtgg
    31921 ggaggcgatg accacggtga tcaagacaat ggacgatatc gccgacagta cctagcgcat
    31981 tggcaccatt acttcgctga ttaacgatat tgcgtttcag accaatattc tggccctgaa
    32041 tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa ggttttgcag tggtggcagg
    32101 ggaagtgcgt catttagcca gccgcagcgc taatgctgcc aacgatattc gcaagctgat
    32161 tgatgccagt gctgataagg tgcaatccgg ttcgcagcag gtacacgccg ccggacggac
    32221 gatggaagat attgtggcac aggtgaaaaa cgtcacccag ttgatcgccc agattagcca
    32281 ttcaacgctg gaacaggccg atgggctttc cagcctgacc cgtgcagtgg atgagcttaa
    32341 cctgatcacc cagaaaaatg ccgagctggt ggaagagagt gcgcaggtgt cggcgatggt
    32401 gaaacaccgc gccagccgac tggaagacgc ggtgacggtg ctgcattaat cggtatgccg
    32461 gatccggcgg ttggagcaca atgcctgatg cgatgctggc gcatcttatc aggcctgcgg
    32521 ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg tcgcatccgg caattgcacc
    32581 gcgccactgg cggatgcggc gattaatcca gttgcgtaat atcaaaagcg gcgcggtcga
    32641 tcaccgcaat gatctttttt atttgtgcat cactgatatc gctctggttg acgcgtaaat
    32701 ccagcaccgc tttaaaatta tccagcgccc gcttcatttg cgggttctgg cgcagcgccg
    32761 cgccaacgca acgcgctttg atgcgttctt caatcatctc cacttgttcg cggttttctt
    32821 ccagccactg cgcgccttgt tcggtcagcg caatctgctt cttaccacct tcctcttcgc
    32881 ggatggtaat cagcgactgc tcctgcagaa aatccagcgt cgggtagatg acgcccgggc
    32941 ttggggtgta attcccctgg gttagattct caatcgcttt aatcaattcg taaccgtggc
    33001 tgtcatcgcg cgagagaata tccagaatca ccagacgcaa ttcaccgtga ccaaagaaac
    33061 gttgccgacg accgccgccg cgaccgtggc gaccaccgca acattgaccg tgttcatgct
    33121 ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc tttgcagcag ccctcatggc
    33181 gtggctggcc ttcatgttta caacaccctt cgtgatgatg gctcatatca gcctcctttt
    33241 gcttgattag atatatctaa tttatatctg atgtgagcat tactgcaagt acaaaaatga
    33301 tttttaaacg tggaagcacc tcacatctag gccggataag acgttcacgc cgcatccggc
    33361 agcattgcta tggcactgaa tgccttcaca tacttcagac atatcagttc tacaaatcgc
    33421 ttgcatttat catgattaac aatcattatc atttgcgaat tttatttaga tatatctgat
    33481 taccatcacg aaggcgataa caatgaataa ccccccccgc tacccgcagc gcgttcgcaa
    33541 tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag cgcatcagcg ccggttttca
    33601 gcgcattgtc cttggcggcg aggcgttgga cggttttaca tcgcgcggct ttgacgatca
    33661 cagcaaactc ttctttcctc aacctgacgc tcactttgtg ccgccaacgg taacggaaga
    33721 gagcatcgtc tggccggaag gaccacgccc accgtcgcgt gactatacgc cactgtatga
    33781 cgaactacgc catgaactgg cgatcgattt ctttattcac gacggtgggg tcgccagcgg
    33841 ctgggcgata aacttacggt ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac
    33901 gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa
    33961 acgttgagca aacttgccgt taaaccgcaa gttagtgcgc tggttagcgt gcgggataac
    34021 gcctgtcagg attatctcgc gcaccttgat ggttttaata tcgaatggct ggcacatgat
    34081 gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga ttacttcatc
    34141 tggataaccg gcgaaggtaa agtcgttaag aatttaagcc gccgctttga agcggaacag
    34201 tatgacccac agcgggtacg tgcagcggct tactggcacg caaagtaact gatatataaa
    34261 aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat ctggtggccc ctgctggact
    34321 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aggggccatg
    34381 gtagcggatt ataaagtaac tccgtgccgc aatccagcca ttaccgcgcg cctgctgttt
    34441 ttataaacaa tgtattttca atccgttata cttttcctgg tgatgttaga aaggagtaaa
    34501 tatggttgag gatattttgg ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg
    34561 gctttcatcc gccgggactg gttttccctt tgctcatccg gcaaatcgct tctggaaggt
    34621 gatatatcag gccgggttta ccgaccgtca gttgaagccg caggaggcac agcatctgct
    34681 ggattatcgt tgtggcatca ccaaactggt agaccgtcca acggtgcaag ccaatgaagt
    34741 ttcaaagcag gagctgcacg caggtgggcg taagctgatt gaaaaaattg aagattatca
    34801 gccgcaggcg ttggctattc tgggcaaaca agcatatgaa cagggattca gctagcgcgg
    34861 tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg cagatttggg tgctgccaaa
    34921 tcccagcggt ttaagtcgcg tttcactgga gaaactggtt gaagcgtatc gcgagctgga
    34981 ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg g
//
LOCUS       DAJTOW010000031        34996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTOW010000031 DAJTOW010000000
VERSION     DAJTOW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.031"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..815)
                     /locus_tag="OO244_001652"
     CDS             complement(<1..815)
                     /locus_tag="OO244_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5663516.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO244_001653"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO244_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="OO244_001654"
     CDS             complement(1398..1874)
                     /locus_tag="OO244_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5663517.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO244_001655"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO244_001655"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5663518.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="OO244_001656"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="OO244_001656"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5663519.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="OO244_001657"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="OO244_001657"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5663520.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="OO244_001658"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="OO244_001658"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5663521.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="OO244_001659"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="OO244_001659"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5663522.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="OO244_001660"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="OO244_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5663523.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO244_001661"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO244_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5663524.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="OO244_001662"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="OO244_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5663525.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="OO244_001663"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="OO244_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5663526.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="OO244_001664"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="OO244_001664"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5663527.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="OO244_001665"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="OO244_001665"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5663528.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="OO244_001666"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="OO244_001666"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5663529.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="OO244_001667"
     CDS             13849..14553
                     /locus_tag="OO244_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5663530.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="OO244_001668"
     CDS             14758..15471
                     /locus_tag="OO244_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5663531.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="OO244_001669"
     CDS             complement(15507..16463)
                     /locus_tag="OO244_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5663532.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO244_001670"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO244_001670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5663533.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="OO244_001671"
     CDS             complement(17701..18462)
                     /locus_tag="OO244_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5663534.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="OO244_001672"
     CDS             18595..19005
                     /locus_tag="OO244_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5663535.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="OO244_001673"
     CDS             complement(18967..20073)
                     /locus_tag="OO244_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5663536.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="OO244_001674"
     CDS             complement(20084..21217)
                     /locus_tag="OO244_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5663537.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="OO244_001675"
     CDS             complement(21210..22946)
                     /locus_tag="OO244_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5663538.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCLDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEINISETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO244_001676"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO244_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5663539.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO244_001677"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO244_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5663540.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="OO244_001678"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="OO244_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO244_001679"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO244_001679"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5663541.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRGSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="OO244_001680"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="OO244_001680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5663542.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="OO244_001681"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="OO244_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5663543.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="OO244_001682"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="OO244_001682"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5663544.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="OO244_001683"
     CDS             complement(31428..31688)
                     /locus_tag="OO244_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5663545.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO244_001684"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO244_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5663546.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO244_001685"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO244_001685"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5663547.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34996)
                     /locus_tag="OO244_001686"
                     /pseudo
     CDS             complement(33012..34996)
                     /locus_tag="OO244_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8295 a         8973 c         9454 g         8274 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgctg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt caaggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa ccccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacta cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca gacgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgc
//
LOCUS       DAJTOW010000032        34895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTOW010000032 DAJTOW010000000
VERSION     DAJTOW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.032"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            846..3131
                     /gene="nrdA"
                     /locus_tag="OO244_001687"
     CDS             846..3131
                     /gene="nrdA"
                     /locus_tag="OO244_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5663548.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKNLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3365..4495
                     /gene="nrdB"
                     /locus_tag="OO244_001688"
     CDS             3365..4495
                     /gene="nrdB"
                     /locus_tag="OO244_001688"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5663549.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4495..4749
                     /gene="yfaE"
                     /locus_tag="OO244_001689"
     CDS             4495..4749
                     /gene="yfaE"
                     /locus_tag="OO244_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5663550.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4803..5453)
                     /gene="inaA"
                     /locus_tag="OO244_001690"
     CDS             complement(4803..5453)
                     /gene="inaA"
                     /locus_tag="OO244_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5663551.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFDRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5632..5874
                     /locus_tag="OO244_001691"
                     /pseudo
     CDS             5632..5874
                     /locus_tag="OO244_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5916..6992)
                     /gene="glpQ"
                     /locus_tag="OO244_001692"
     CDS             complement(5916..6992)
                     /gene="glpQ"
                     /locus_tag="OO244_001692"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5663552.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6997..8355)
                     /gene="glpT"
                     /locus_tag="OO244_001693"
     CDS             complement(6997..8355)
                     /gene="glpT"
                     /locus_tag="OO244_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5663553.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8628..10256
                     /gene="glpA"
                     /locus_tag="OO244_001694"
     CDS             8628..10256
                     /gene="glpA"
                     /locus_tag="OO244_001694"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5663554.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10246..11505
                     /gene="glpB"
                     /locus_tag="OO244_001695"
     CDS             10246..11505
                     /gene="glpB"
                     /locus_tag="OO244_001695"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5663555.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11502..12692
                     /gene="glpC"
                     /locus_tag="OO244_001696"
     CDS             11502..12692
                     /gene="glpC"
                     /locus_tag="OO244_001696"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5663556.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12885..13811
                     /locus_tag="OO244_001697"
     CDS             12885..13811
                     /locus_tag="OO244_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5663557.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13852..14322)
                     /locus_tag="OO244_001698"
     CDS             complement(13852..14322)
                     /locus_tag="OO244_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5663558.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14377..15579)
                     /locus_tag="OO244_001699"
     CDS             complement(14377..15579)
                     /locus_tag="OO244_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5663559.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15666..16208)
                     /locus_tag="OO244_001700"
     CDS             complement(15666..16208)
                     /locus_tag="OO244_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663560.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16487..16912
                     /gene="nudI"
                     /locus_tag="OO244_001701"
     CDS             16487..16912
                     /gene="nudI"
                     /locus_tag="OO244_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5663561.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSCGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16951..17553)
                     /locus_tag="OO244_001702"
     CDS             complement(16951..17553)
                     /locus_tag="OO244_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5663562.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17861..19000
                     /gene="arnB"
                     /locus_tag="OO244_001703"
     CDS             17861..19000
                     /gene="arnB"
                     /locus_tag="OO244_001703"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5663563.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19004..19972
                     /gene="arnC"
                     /locus_tag="OO244_001704"
     CDS             19004..19972
                     /gene="arnC"
                     /locus_tag="OO244_001704"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5663564.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19972..21954
                     /gene="arnA"
                     /locus_tag="OO244_001705"
     CDS             19972..21954
                     /gene="arnA"
                     /locus_tag="OO244_001705"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5663565.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYHIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21951..22841
                     /locus_tag="OO244_001706"
     CDS             21951..22841
                     /locus_tag="OO244_001706"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5663566.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22841..24520
                     /gene="arnT"
                     /locus_tag="OO244_001707"
     CDS             22841..24520
                     /gene="arnT"
                     /locus_tag="OO244_001707"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5663567.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLVAGMCSFWL
                     AMQAQTWKGKSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGW
                     LAVISCVLTVLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAG
                     SLPWLGLLPGALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAM
                     LLAHYALLAAKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVF
                     CAWSIFSLWAFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEM
                     TQESLQPSRYILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDE
                     FANWLNQHRQEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24517..24852
                     /gene="arnE"
                     /locus_tag="OO244_001708"
     CDS             24517..24852
                     /gene="arnE"
                     /locus_tag="OO244_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5663568.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24852..25238
                     /gene="arnF"
                     /locus_tag="OO244_001709"
     CDS             24852..25238
                     /gene="arnF"
                     /locus_tag="OO244_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5663569.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25232..25504)
                     /gene="pmrD"
                     /locus_tag="OO244_001710"
     CDS             complement(25232..25504)
                     /gene="pmrD"
                     /locus_tag="OO244_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5663570.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25609..26964)
                     /gene="menE"
                     /locus_tag="OO244_001711"
     CDS             complement(25609..26964)
                     /gene="menE"
                     /locus_tag="OO244_001711"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5663571.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26961..27923)
                     /gene="menC"
                     /locus_tag="OO244_001712"
     CDS             complement(26961..27923)
                     /gene="menC"
                     /locus_tag="OO244_001712"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5663572.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27923..28780)
                     /gene="menB"
                     /locus_tag="OO244_001713"
     CDS             complement(27923..28780)
                     /gene="menB"
                     /locus_tag="OO244_001713"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5663573.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28795..29553)
                     /gene="menH"
                     /locus_tag="OO244_001714"
     CDS             complement(28795..29553)
                     /gene="menH"
                     /locus_tag="OO244_001714"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5663574.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29550..31220)
                     /gene="menD"
                     /locus_tag="OO244_001715"
     CDS             complement(29550..31220)
                     /gene="menD"
                     /locus_tag="OO244_001715"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5663575.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31309..32604)
                     /gene="menF"
                     /locus_tag="OO244_001716"
     CDS             complement(31309..32604)
                     /gene="menF"
                     /locus_tag="OO244_001716"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5663576.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32683..32988)
                     /gene="elaB"
                     /locus_tag="OO244_001717"
     CDS             complement(32683..32988)
                     /gene="elaB"
                     /locus_tag="OO244_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5663577.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33043..33504)
                     /locus_tag="OO244_001718"
     CDS             complement(33043..33504)
                     /locus_tag="OO244_001718"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5663578.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33569..34486
                     /gene="rbn"
                     /locus_tag="OO244_001719"
     CDS             33569..34486
                     /gene="rbn"
                     /locus_tag="OO244_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5663579.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34677..34865
                     /locus_tag="OO244_001720"
                     /pseudo
     CDS             34677..34865
                     /locus_tag="OO244_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8533 a         9338 c         8647 g         8377 t
ORIGIN      
        1 aaggctttgc ctgctggcgt gacaactcgc tttgatatca tatccctggc ataaatcaat
       61 ggccgccgcg acaccgttag cagagaaaag agatgcaatc attgacggga gtagagataa
      121 atactccttg cgtagaaaga taatccgcat attaatctga ctatcatttt atataagacg
      181 gcatatttaa acgcattata caatatgggt tatctttctt ttgtaaattg tccaacccga
      241 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
      301 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      361 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      421 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      481 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      541 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      601 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      661 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      721 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      781 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      841 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      901 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      961 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
     1021 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
     1081 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
     1141 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
     1201 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1261 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctagtacaga
     1321 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1381 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttatgacg
     1441 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1501 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1561 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1621 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1681 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1741 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1801 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1861 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1921 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1981 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     2041 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     2101 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     2161 gcctagaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     2221 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2281 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2341 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2401 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2461 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2521 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2581 cgatcgatac ctataagaaa aatctggatg ccatcgctaa tgagccgctg cattacgact
     2641 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2701 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2761 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2821 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2881 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2941 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     3001 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     3061 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     3121 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     3181 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3241 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3301 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3361 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3421 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3481 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3541 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3601 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3661 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3721 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3781 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3841 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3901 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3961 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cctttgcctt
     4021 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     4081 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     4141 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     4201 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4261 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4321 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4381 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4441 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4501 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4561 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4621 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4681 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4741 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4801 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4861 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4921 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4981 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     5041 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     5101 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     5161 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     5221 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggtc gaacggataa
     5281 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5341 tcgacgcatt gcaccccgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5401 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5461 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5521 aaacgtgtcg tattaatgaa tttcttttgc cgctacaggt gtgacaccgc ttttgccctt
     5581 catttgcagc gcatagattc caccagccac taccactaat agtgcgccca gcagataaaa
     5641 tgtgaattta atcagtcgga tacagcgatg aattttattc gacagggatt aggcatcgcc
     5701 ttacaaccag agttaacgct gaaaagcatt gcaggtgaat tgtgttccgt tcctcacgaa
     5761 ccaaccttct atcgacagat ttcgttgctg actaaagaaa agccggtaga aggcagtcca
     5821 ctgtttttac tacaaatgtg catggaacaa ttagtggcga ttggaaaaat ttgatatgag
     5881 acaggatggc gcatgaacgc catcctgcag taacattact ctttattaag aaattttact
     5941 gccttatcag ggaaatcagt aaacagccca tttacacccg ctttgttata cagagcatca
     6001 tataactgat tcacatcagg agtgtattca ggcagtttat ctgaccgcac ggtataagga
     6061 tgcactacca gtttattctg ctgggcatct tgcaccatgc cagtgagttt gatattaccc
     6121 ggctgcgatg tctcctcaat caacatatgg taatccggac caataccatc tgcatattcc
     6181 gccacctgtt tcatggctcc cggcttaaac atccagtcgt agttgtaatt aacccagctt
     6241 ccatccggct gtttctgctg cgtttcattc cagtcggtat aggcaatcag ctgtaccaga
     6301 ttgagctcca tgcccatttt gggttccagc tcattcttaa tacgcttcag ctcatcagca
     6361 tcaaaacatt gcaaataaac tttatcgtct ttaccggtgt aaccatattt cttcagcact
     6421 tccagcgttt ttgccgcaat atccttccct tcctgatgat ggaaccacgg cgctttgatt
     6481 tctggataga taccgatatt tttcccggta gagtgattta acccctgaac aaattcaatc
     6541 tcttcttcaa aggtgtgcac ccggaagtcg gacttaccca ttgggaaacg ccccggataa
     6601 gtctgtactt ttttaccgtt ttcaatatcg aaaccttcgg taaatttcag cgacttaatt
     6661 tcatccagcg tgaaatctat cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa
     6721 cgatcggcaa catcagtaac acgatcgaga taatggtcat gcagaacaac cagatggtcg
     6781 tctttggtca tcaccaaatc ctgttccaga taatccgctc cctgcgcata cgccatcgct
     6841 tttgctggca gcgtatgctc cggcaaatat ccactggcac cgcgatgggc gatgactatt
     6901 ttttcgttgc tgtccgccgc cattgcactg cttcccatga ctatagtgct catcatgatc
     6961 gccatgctaa ggtttttcag cttcaatttc atgccattag cctccgttgc gtttttgcag
     7021 taattgttca tggcgacgtt tttcgccaat catcacaaca atcaacaaga taaccgccag
     7081 aatgctgccg ccaatcatta ccataaagcc gccatcccag ccgaagaagt ccacggtgta
     7141 gccaacaatc gcgctcgccg ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc
     7201 cgctgccgta cctgccgctt ttttcggtgc cagttccagc gcatgcagac cgatcagcat
     7261 cacaggaccg tagatcagga agccgataac aatcatacaa atcatatcga cggttgggtt
     7321 acctgccggg ttcatccagt aaacgatagt cgcgatggtc accagtgtca taaagaagac
     7381 gccggttgcc ccacggttac cacggaagac tttgtccgac atccagccgc acagcagggt
     7441 gcccggaata cccgcatact catagaagaa gtaggcccag gaggatttat ccagcgcgaa
     7501 atgcttaacc tctttcagat aagtcggtga ccagtcgagg atgccgtaac gcagcagata
     7561 aacgaacacg ttggcgatgg cgatgtacca cagcagtttg ttcggcagca cgtactgcat
     7621 gaagatttgc ttcgcggtca gctcctgttc cgctttttcg ttatagtcgt ccggataatc
     7681 atttttgtac tcttcgatcg gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc
     7741 aaaggcgaat aatgccacca gaatggcgca gaaagcaggc atatagagcg ccgcatgcca
     7801 gtcattgaac caggccatcc ccagcaggaa cagcagcggc ggaataccac caccgacgtt
     7861 gtgcgcacag ttccacactg acacaatgcc gccacgttct ttctgcgacc accagtgcac
     7921 catagtacga ccacacggcg gccaccccat cccctggaac caaccgcaga ggaacaacag
     7981 tacaaacatc accgcaatgc tcgacgtcgc ccatggcaca aagcccataa acaacatcac
     8041 tgccgccgcc agaatcaaac ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac
     8101 cgaacccatg atgaatttcg aaaatccata agcaatcgag atccccgaaa gggcaaaacc
     8161 taaatcaccg cgtgagaatc cctgctcaac cagataaggc atagcaagcg caaagttctt
     8221 acgaaccaaa tagtaagccg catagccaaa gaatatcccc aggaaaattt gccagcgcaa
     8281 tcgacgataa gtcggatcga tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt
     8341 aaaaatactc aacattgata gcctccgtgg cccgtggtct tatttatgat taacagcctg
     8401 attcagtgag agaacctgcc gtttcttgag ttgccgcgat gttaagaaaa cattcataaa
     8461 ttaaatgtga attgccgcac acattattaa ataagattta caaaatgttc aaaataacgc
     8521 atgaaatcac gttttacttt cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg
     8581 tttttactat ggctaaatgg taaaaaacga acttcagagg gataacaatg aaaactcgcg
     8641 actcgcaatc aagtgacgtg attatcattg gcggcggcgc aacgggagcc gggattgccc
     8701 gcgactgtgc cctgcgcggg ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg
     8761 gtgccaccgg gcgtaaccac ggcctgctgc acagcggtgc gcgctatgca gtaaccgatg
     8821 cggaatcggc ccgcgaatgc attagtgaaa accagatcct gaaacgcatt gcacgtcact
     8881 gcgttgaacc aaccaacggc ctgtttatca ccctgccgga agatgacctc tcattccagg
     8941 ccacttttat tcgcgcctgc gaagaagcag ggatcagcgc agaagctata gacccgcagc
     9001 aagcgcgcat tatcgaacct gccgttaacc cggcactgat tggcgcggtg aaagttccgg
     9061 atggcaccgt tgatccattt cgtctgaccg cagcaaacat gctggatgcc aaagaacacg
     9121 gtgccgttat ccttaccgct catgaagtca cggggctgat tcgtgaaggc gcgacggtgt
     9181 gcggtgttcg tgtacgtaac catctcaccg gcgaaactca ggcccttcat gcacctgtcg
     9241 tggttaatgc cgctgggatc tgggggcaac acattgccga atatgccgat ctgcgcattc
     9301 gcatgttccc ggcgaaagga tcgctgctga tcatggatca ccgcattaac cagcatgtga
     9361 tcaaccgctg ccgtaaacct tccgacgccg atattctggt gcctggcgat accatttcgc
     9421 tgattggtac cacctcttta cgtattgatt acaacgagat tgacgataat cgagtgacgg
     9481 cagaagaggt tgatattctg ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa
     9541 cgcgcatttt gcgggcctat tctggcgtgc gcccgctggt tgccagcgat gacgacccga
     9601 gcggacgtaa cgtcagccgt ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg
     9661 acggatttat caccatcacc ggtggcaaac tgatgaccta tcggctgatg gctgaatggg
     9721 ctaccgacgc ggtatgccgc aaactgggca acacgcgccc ctgtacgact gccgatctgg
     9781 cactgcctgg ttcacaagaa cccgctgaag ttaccttgcg taaagtcatc tccctgcctg
     9841 ccccgctgcg cggttctgcg gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg
     9901 aaggccgtct acaccgtagc ctggtatgtg agtgcgaagc ggtaactgcg ggtgaagtgc
     9961 agtacgcggt agaaaattta aacgttaata gcctgctgga tttacgccgt cgtacccgtg
    10021 tggggatggg cacctgccag ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac
    10081 ggtttaacgt cacgacgtcc gcgcaatcta tcgagcaact ttccaccttc cttaacgaac
    10141 gctggaaagg cgtgcaaccc atcgcctggg gagacgcact gcgcgaaagc gaatttaccc
    10201 gctgggttta tcagggactg tgtggtctgg agaaggagca gaaagatgcg ctttgatact
    10261 gtcattatgg gcggcggcct cgccggatta ctctgtggcc tgcaactgca aaaacacggc
    10321 ctgcgctgtg ccattgtcac gcgtggtcaa agcgcgttgc atttctcatc cggttcgctg
    10381 gatttgctga gccatctgcc agatggtcaa cccgtggcag acattcacag tggactggaa
    10441 tctttgcgtc agcaggcacc tgcacatcct tactctcttc tcgggccaca acgcgtgctc
    10501 gatctcgctt gccaggcgca ggcattaatc gctgaaagcg gtgcgcaatt gcagggcagc
    10561 gtagaactgg ctcaccagcg gattacgccg ctcggcactt tgcgtgcaac ctggttaagt
    10621 tcgccagaag ttcccgtctg gccgctaccc gcgaagaaga tatgtgtagt ggggataagc
    10681 gggctgatgg atttccaggc gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg
    10741 gttgaaaccg cagaaataga gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa
    10801 tttcgcgcgg tgaatatcgc ccgtttcctt gataatgaag aaaactggcc gctgttactt
    10861 gatgcgctta ttcctgtcgc caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc
    10921 ctggccgatg acaaactgtg gcgttggttg aatgaaaaac taccttgttc actgatgctt
    10981 ttgccaacgc tgccgccttc cgtgctgggc attcgtctgc aaaaccagtt acagcgccag
    11041 tttgtgcgcc aaggtggagt gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa
    11101 aatggcgtag tgaatgaaat ctggacccgc aatcacgccg atattccgct acgtccacgt
    11161 ttcgcggttc tcgccagcgg cagtttcttt agtggcggac tagtagcgga acgtaacggc
    11221 attcgcgagc cgattctcgg ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat
    11281 aagggcgatt tttttgcgcc gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg
    11341 ctacgcccgt cgcaggcagg gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg
    11401 ggcggatttg atcccatcgc ccagggatgc ggcggcggtg tttgtgccgt cagtgcttta
    11461 catgccgctc aacagattgc ccaacgcgca ggaggccaac aatgaacgac accagcttcg
    11521 aaaactgcat taagtgcacc gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg
    11581 gttatccagg gccaaaacaa gccgggccgg atggcgagcg tctgcgtttg aaagatggcg
    11641 cactgtatga cgaggcgctg aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc
    11701 cgtccgatgt gaagattggc gatattatcc agcgcgcgcg ggcgaaatat gacaccacgc
    11761 gcccgtcgct gcgtaatttt gtgttgagtc ataccgacct gatgggtagc gtttccacgc
    11821 cgttcgcacc aatcgtcaac accgctacct cgctgaaacc ggtgcggcag ctgcttgatg
    11881 cggcgttaaa aatcgatcat cgtcgcacgc taccgaaata ctccttcggc acgttccgtc
    11941 gctggtatcg cagcatagcg gctcagcaag cacaatataa agaccaggtc gctttctttc
    12001 acggctgctt cgttaactac aaccatccgc agttaggtaa agatttaatt aaagtgctca
    12061 acgcaatggg taccggtgta caactgctca gcaaagaaaa atgctgcggc gtaccgctaa
    12121 tcgccaacgg ctttaccgat aaagcacgca aacaggcaat tacgaatgta gagtcgatcc
    12181 gcgaagctgt gggagtaaaa ggcattccgg tgattgccac ctcctcaacc tgtacatttg
    12241 ccctgcgcga cgaatacccg gaagtgctga atgtcgacaa caaaggcttg cgcgatcata
    12301 tcgaactggc aacccgctgg ctgtggcgca agctggacga aggcaaaacg ttaccgctga
    12361 aaccgctgcc gctgaaagtg gtttatcaca ctccgtgcca tatggaaaaa atgggctgga
    12421 cgctctacac cctggagctg ttgcgtaaca tcccggggct tgagttaacg gtgctggatt
    12481 cccagtgctg cggtattgcg ggtacttacg gtttcaaaaa agagaactac cccacctcac
    12541 aagccatcgg cgcaccactg ttccgccaga tagaagaaag cggcgcagat ctggtggtca
    12601 ccgactgcga aacctgtaaa tggcagattg agatgtccac aagtcttcgc tgcgaacatc
    12661 cgattacgct actggcccag gcgctggctt aaactccctt ctgatgcccg gtaagcatgt
    12721 ggttaccggg catttttgcg tacacgattc cgtgcccaat gtatgcgttg caacgcagtg
    12781 aaaattcctc tgaaaacgtc tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat
    12841 aaccgtaatg tcgcgtcatc ataaatatca ggtgacggac aaccatgacc gaatcaacaa
    12901 cctcctcccc gcatgatgcg gtatttaaaa cctttatatt cacacccgaa accgcacggg
    12961 attttctcga aatacattta ccagaaccac tgcgcaagct ttgcaacctg caaaccttac
    13021 gcctggaacc cactagtttt attgaaaaaa gtttacgcgc ttactactcg gatgttttgt
    13081 ggtccgtgga aaccagcgac ggtgacggct atatctactg cgtgattgaa catcaaagct
    13141 ctgcagaaaa gaatatggct tttcggccaa tgcgctatgc cactgccgcc atgcagagtc
    13201 acctagataa aggctatgac agagttccgc tggtggtgcc attgctgttt tatcatggcg
    13261 aaacatcgcc ctacccgtac tcacttaact ggctggatga gtttgacgat ccgcaacttg
    13321 cccggcagtt gtacaccgaa gcttttccgt tggtggatat caccatcgta cctgacgatg
    13381 agatcatgca acatcggcgt atagctctgc tggaactgat tcaaaagcat attcgcgacc
    13441 acgatttaat cggcatggtc gacaggatca ccacgctttt ggttagaggc ttcactaatg
    13501 acagccagct acaaacactg tttaattatc tgctgcaatg cggcgatacc tcccgtttca
    13561 cccgttttat tcaggagatt gccgaacgtt caccactaca aaaggagaga ttaatgacta
    13621 ttgctgaacg gctacggcag gaaggacatc aaattggctg gcaagaaggt aaattagaag
    13681 gtttgcagga aggtatgcat gaacaagcca ttaaaattgc tttgcgcatg ctggaacagg
    13741 gcattgatcg tgaccaagtg ctcgcggcca cccagctaag cgaagccgat ctggcagcga
    13801 ataaccacta attaacacag gcccacagcc gatccccatg ggcctttgat atcaataact
    13861 accttttatg cgtggcccat ttttgcctga tttaaacatc gccagtcgtt gatccagggc
    13921 atcgctgtag agcatcgtgt caacgccaac agcgacaaag ttcgctcccc acgccaggca
    13981 ttgctgcgcc atatcaggag ccacagccag aaaaccagcc gctttacccg cagcacggat
    14041 ccgccgaatg ctggtttcaa taattcgctg cacttccggg tgcccggcgt tatccgggta
    14101 gcccaacgac gcagaaagat ccgctggtcc aataaacacg ccatcaatcc cttcgacgtc
    14161 gaggatctcg tccaggttat ccagtgccgt tttactttcc acctgcacta acagacaaag
    14221 cgaatcgttg acttgcgcca tgtaattctc aatgcgtccc cagcgcgcag cccgtgccac
    14281 actggcccca acaccacgct caccgtaggg agggttggac aaattttgcc ggagggggaa
    14341 tgtgccggtg cagtaaaccg gcacagagtg gcgaaatcag acggataaag tcatggactc
    14401 aacaacctca atccagccat gctcactagc gatgtcctgg ccgtttaacc aacggcgtag
    14461 catattcagt gccatcattg cgcacacttc ctgacggata gccaggctgt agcgtgtagt
    14521 gctgaaacgc acacgcagag cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg
    14581 ctcgttctcg aaacccgaaa cagccagtgc cagcccggca aaatggttgg cccgccgttc
    14641 tgtaatccag tgcgcagttt gcgccagggt ttcttcctgt gaaggaatca cttcacacgc
    14701 cagcaatgga gcacctgcgc gagaaagttg caaagccaat aaaccaccgg tgaattgctc
    14761 gctcaacgtc aggctgaact ggcggttttg caattcgcga ctgatctgcg cgggcagtcc
    14821 ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt
    14881 ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga tcggcattga
    14941 ggagcgatag cccattgtta cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag
    15001 atcgctttcc gaacgaccaa aggtagttaa acgcagacaa accggcggct gtggtaaaga
    15061 aaaacgctcg cgcaggcgcg gcaggatttc gtgctcgacc atcaccttaa attctgacgg
    15121 tacgccggga gtaaagaaca tcaggcaacg attaagctgc acggcaaaac cacaggcggt
    15181 gcctaccggg ttattgataa attcagcact ggcaggcagc tccgcttgtt tacggttgct
    15241 cggtgccatt actcgtccac gttcgtggaa atagcgttcc atctctttga gccaggcttc
    15301 atgcagcacc aggccttcac cttttgctgt cgcagcggcg agtgcgctta aatcatcgct
    15361 ggtcggtccc agcccgccgt taacgatcag cacatcggcg tgctgactac gttcgcgaag
    15421 aatggtgact aagtcatcaa ggttatcccc caccgtattg cggcgagata atggcagccc
    15481 ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt gcccgtataa
    15541 cacttcatcc ccggtggata gcatttccac ttttaacatg gttatctcct gcttttgtta
    15601 tcgaatcact ataacgcaaa gggagataag gcgtagtaag aaactgaatg ccgcgccgga
    15661 agggatcaga aactggcgtt aaccccaaca tacaggccgt cagccacagc gttgtcgcgg
    15721 ttaccgtctt tacccgacag attcaggtag cgataacccg cctcaatact gactggacgc
    15781 ataatggtgt aacgcgcgcc agcattcgct tcctcataac tttgaatacc gctggagagc
    15841 gaatccggag agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc
    15901 aaaccacctc ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtacacg
    15961 ccttttccgc caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg
    16021 cccgcgacgt caccgtcgtc gtcgttatgt gtccagttac cgcttaaagc caggcccgtc
    16081 gattcagtcc caaaaccgac accaatattg gtgtattctt tacccgcctg gccgctgatg
    16141 ctcattgcat tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt
    16201 tttttcatta tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt
    16261 actgcctggc acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta
    16321 aatacttctt aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa
    16381 ataataataa gtgcagttat cattgcaaaa gagattactg tcactttcac atatttctga
    16441 gttaccgtat tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac
    16501 tattgtatgc cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga
    16561 tcgcggcgtt ttccccggtc aatgggcgct ttcgtgtggc ggcgtggagt ctggcgaacg
    16621 aattgaagag gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac
    16681 agaaatcacg ccgtggacct tcagcgatga tattcgcacc aagacatatg cagatggtcg
    16741 caaggaagag atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt
    16801 gaaaataaac gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta
    16861 tgatttaaat gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg
    16921 acagccatca ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata
    16981 aactttgcct ttttccacat gcatgactaa accatccaga taatcaggtt taaatatcgc
    17041 atcacgctta tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt
    17101 tttatcaggc gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg
    17161 aagaagtcgt ttatctaccg tcagcttttt acccgcggaa aaccaggtcg ccgactgaat
    17221 ggtccggacg gtattactgg aataaagatc gaaatcagag atgtcagcgc taaaagcgtt
    17281 gcccagttca cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca
    17341 ttgattggtt gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg
    17401 ctgtgccagt ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc
    17461 ggcatgagta cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt
    17521 tgacttcaat gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa
    17581 ccagacaatt accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata
    17641 ttaaaaatcc tacgatgtcg gcaaaatgcc tcaaattttt gccaaatgca aagcctaaat
    17701 aagaaaaaat ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta
    17761 atattctatc cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca
    17821 aatataagtg gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt
    17881 ttcgcgacca gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg
    17941 ttggatcaca accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg
    18001 aaatcagcat gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc
    18061 gttggaaatt ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct
    18121 caatatgatt tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct
    18181 gatggtcacg cctgaagcga tcgaatcagc cattacgcca cgcactaaag ccatcattcc
    18241 ggtgcattat gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta
    18301 cggcatcgca gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca
    18361 tattggcgca aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc
    18421 tgaaggtggc ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa
    18481 atttcacggt ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc
    18541 tgaagtctta acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct
    18601 gacacagtta gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg cccagcaata
    18661 tcagcaagca ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt
    18721 tcacgcctgg catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga
    18781 tgcgttgatg gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc
    18841 tcacacacaa aaatattatc gcgagcgttt tcccacgctg tcgttaccga ataccgaatg
    18901 gaatagcgaa cgcatctgtt cgttgccgct gttcccggat atgactaccg ccgatgccga
    18961 ccgcgtcatc acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc
    19021 tgttaagaaa gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt
    19081 aatcaggcgc accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat
    19141 tgatgacggc agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa
    19201 cagccatatt gtgtctattt tgattaaccg caattacggg caacattcag cgattatggc
    19261 gggattcagt cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc
    19321 gccagaagaa atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac
    19381 tgtacgccag aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaaccg
    19441 gcttattcag cgcaccactg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta
    19501 tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa cgcagcacct ttatcccgat
    19561 tctggcgaat atcttcgccc gccgtgcaat tgaaattcca gtacatcatg ccgagcgtga
    19621 gtttggtgaa tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac
    19681 ctgccttacc acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg
    19741 aggttttagc attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc
    19801 ggcagaaggc gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat
    19861 cggcatggga ttactcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc
    19921 ccgctatttt gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc
    19981 gtcgtttttg cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt
    20041 tacgaaatta gcgccatttt tacccatacc gataatcccg gtgaaaaagc cttttatggt
    20101 tcggtggctc atctggcggc ggaaagagac attccggttt atgcgccgga taacgttaat
    20161 catccgctgt gggtggaacg cattgcccaa ctgtcaccag aggtgatttt ctctttttat
    20221 tatcgccatc ttatttgcga cgagattttt cagctcgctc ccgcaggtgc atttaatctg
    20281 cacggttcgc tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac
    20341 ggtgaaacgg aaacgggcgt tacattgcac cgaatggtaa aacgtgccga tgctggggcc
    20401 attgtggcgc aactgcgcgt tgccattgcg ccagacgata tcgctattac gctgcatcat
    20461 aaattgtgcc atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt
    20521 aatattctgg aaatcgccca gcgcgaaaac gaagccacct gttttggtcg cagaacgccg
    20581 gatgacagtt tccttgaatg gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc
    20641 gttgccgatc cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg
    20701 tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg
    20761 ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac
    20821 ggcattacta tgcagggctc gcaattagcg cagatgctgg gcctggtgca aggttcacgc
    20881 ttgaatagcc agcctgcctg caccgcccga cgccgtaccc gggtactcat cctcggggtg
    20941 aatggcttta ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt
    21001 tacggtctgg atattggcag cgatgcgata agccgttttc tgaatcatcc acattttcac
    21061 tttgtcgaag gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt
    21121 gatgtcgtct tgccgctggt ggcgatagcc acgccgattg aatatacccg caacccgctg
    21181 cgcgtatttg aactcgattt tgaagagaat ctgcgcatta tccgctactg cgttaagtac
    21241 cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg ggatgtgtag cgataaatac
    21301 ttcgatgagg accattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat
    21361 tcggtgtcaa aacaattact tgatcgggtg atctgggcct atggcgaaaa agagggttta
    21421 cagttcaccc tcttcctccc gtttaactgg atgggaccac gactggataa ccttaatgca
    21481 gcgcgaattg gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca
    21541 ccaattaagc taattgatgg cggaaaacaa aaacgctgct ttactgatat ccgggacggt
    21601 atcgaggcgt tgtaccacat tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc
    21661 aacattggca atcctgagaa cgaagcgagc attgaggaac tgggcgagat gctgctggcg
    21721 agcttcgaaa aacatccgct gcgccatcat ttcccaccgt ttgcgggctt tcgcgttgtc
    21781 gaaagtagct gctactacgg caaaggatat caggacgtag agcatcgtaa accgagcatc
    21841 cgcaatgccc accgctgcct ggactgggag ccgaaaattg atatgcagga aaccatcgac
    21901 gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag
    21961 taggcttacg cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc
    22021 tggaaatctt gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca
    22081 atatgggccg ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct
    22141 caaacgcggc atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta
    22201 aagagattgg tcatgccaat gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg
    22261 gtctgcacgc ctgggatcac catgcctggc aagcccgtag cggtaactgg gatcggcaaa
    22321 caatgatcga cgatattgca cgcggtcttc gcactctgga agagattatc ggtcaaccgg
    22381 taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag
    22441 cgttccattt gcgctacaac agcgattgtc gtggggccat accgttccgt ccactgcttg
    22501 aatcaggaaa ccctggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga
    22561 ttggtcggga tgtgaaagca gaagatttta acggttggtt actcaaccgc atcctgcgag
    22621 ataaaggcac gccggtttat accattcatg cagaagttga aggctgcgct tatcagcata
    22681 attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg
    22741 aactgttatc agagacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg
    22801 aaggctggct gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg tacgttacct
    22861 tatcggcatc ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct
    22921 ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg catccggcga
    22981 ctggattgtg ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg
    23041 gattaacagc attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt
    23101 tatctttgcg accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg
    23161 cgataaacgt ctggctctac tcgccacagt aatttatctc tcattgttta ttgtctatgc
    23221 catcggcact tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg
    23281 cagcttctgg ctggtggcgg gaatgtgcag cttctggctg gcaatgcagg cacagacgtg
    23341 gaaaggcaaa agcgcaggat ttttactgct gggaatcacc tgcggcatgg gggtgatgac
    23401 caaaggtttt ctcgcccttg ccgtgccggt attaagcgtg ctgccatggg tagcgacgca
    23461 aaaacgctgg aaagatctct ttatttacgg ctggctggcg gttatcagtt gcgtactgac
    23521 ggttctcccc tggggactgg cgatagcgca gcgggagcct gacttctggc actatttttt
    23581 ctgggttgag catattcaac gctttgcact agatgatgcc caacatagag ctccgttctg
    23641 gtactacgtg ccggtcatca ttgccggtag cctgccgtgg ctgggattac tccccggtgc
    23701 actgtacaca ggctggaaaa accgcaagca ttccgcaacc gtctatttgt tgagctggac
    23761 gataatgccg ctgctgtttt tctccgtcgc taaaggcaaa ttgcccacct atattctttc
    23821 ttgctttgca cctctggcaa tgctgttggc gcattacgcg ttgctggcag caaaaaataa
    23881 tcctctggcg ctgcggatta atggctggat taacatcgct tttggcgtca ctggcattat
    23941 tgccacattt gtgatctccc catggggacc aatgaacacg ccggtgtggc aaaccttcga
    24001 gagctataaa gtcttttgtg cctggtcgat tttttcgcta tgggcatttt tcggctggta
    24061 caccttaaca aacgtcgaaa agacctggtc tttcgccgcg ctttgcccgc tggggctggc
    24121 gttgctggta ggattttcaa ttcctgacag agtcatggaa ggaaaacatc cgcaattttt
    24181 tgtcgagatg acacaagaat cacttcagcc aagccgctat attcttactg atagcgtcgg
    24241 tgttgccgca ggtctggcat ggagcctgca acgcgatgac atcatcatgt atcgccagac
    24301 aggtgagttg aaatacggcc ttaattatcc ggatgcgaaa gggagatttg tcagcggtga
    24361 tgagttcgca aactggctta atcaacatcg tcaggagggg attattacac tcgtgctttc
    24421 ggttgaccgc gatgaagata tcaacagtct cgccattccg cccgcagatg ccatcgttcg
    24481 tcaggagcgt ctggtgctga ttcagtatcg tcccaaatga tctggctaac attagtcttt
    24541 gccagcttgc ttagcgttgc cgggcagttg tgtcagaaac aggcaacctg ctttgtggcg
    24601 ataaacaaac ggcgcaaaca tatcgtgctg tggctgggac tggcgctggc ttgtcttggt
    24661 cttgccatga tgctctggct gctggtcttg cagaacgtac cggtaggcat tgcctacccg
    24721 atgttaagtc tgaattttgt ctgggtgacg ctggctgcag taaaactgtg gcacgaaccg
    24781 gtatcgccgc gtcactggtg tggggtggcg ttcattattg gtggcattgt gatcctcggg
    24841 agtacggtgt aatgggcctg atatggggat tattcagcgt cattattgcc tcagttgcgc
    24901 aattaagcct gggttttgcg gcaagtcatc tgccgccgat gacgcactta tgggatttta
    24961 tcgcggctct gttagctttt ggcctggatg cccggatact gttactcggg ctgctgggtt
    25021 atctgctttc cgttttttgt tggtataaaa cgctacataa acttgcgcta agcaaagcct
    25081 atgccttgct cagtatgagt tatgtgctgg tatggattgc ttcaatggta cttcccggct
    25141 gggaagggac tttttcgctg aaagcactac tgggagtagc ctgtattatg agcgggttga
    25201 tgctgatttt cctgcccacg acaaaacaac gttactgagt tttccctgcc gctgtacact
    25261 gccgttccca ctcatcgggg ctataactgc ttgccgagag gactttcacc gtatgcagct
    25321 tttcacgatt aatacaataa agcgcattct gcaaaggcga gagtaaatcc cccactttca
    25381 cggcgaagtc actcttcact tcggcgatca tttttatcgc gccgccagca tcgcagagca
    25441 taagtacatg tctgttatct tgtttattgc agcacgattt tttgtgccag ccattccatt
    25501 gcattatcct gtttgctaag agttttcaca tcaatttaca gattaacaga taaactcttt
    25561 atcaacggca atggaatggc agaaatgaga agtgaaacgg agggttaatt attgctgacg
    25621 ttgcacccac tcttttagcg cctgacgtga aattttaata ccgccgtttt tcagctccgg
    25681 cggtaaagtc agccagcgca ccggttgttg aaaacgagcc agcttatctt tcacccattc
    25741 gccaagatca acggtttgct ggtcatactc caccaccgct accggccgat gaccaaactc
    25801 cttgtcggca acggaaacga taaacacctg caaaaccgca ggatgtgcag caataacacg
    25861 ctcgacttct tccggctgaa taccctcacc gccactgaag aacagattgt ctaaacgtcc
    25921 gacaatggtc agcttgccat tatgcatctc accgcgatcg cgcgtagcgt accagccttc
    25981 gtcattaacc agtgaaacca gttgcccgtt acgccagtaa ccttctgcca tactggcagc
    26041 ccgcagccac acttcattat taacgatttt cacttcccga cccggcagcg gcaaaccaac
    26101 gtctgccagg ccgtcggctt ctttcgcaca taccgtggag gcaaactcgg tcagaccata
    26161 gccgcaaaag caacgaatcc cctgctcgcg cgcctgttcc gtcaactcga ccgggatagc
    26221 cgcgccgcca agtaacaccg ctttcaggga aacggaactg cggttaacca gcaaacgcca
    26281 gagttgtgtt ggcaccagtg aagcgtgagt acagcctgcc agcatttgct ccaatggctg
    26341 tttatcacgc accgtcatcc gcgcaccagc gtataaccag cgccacataa ttccctgacc
    26401 ggagacgtga aacagcggta aagagagcaa ccagtcatcg tgatcgccaa acggaatcag
    26461 cgataacaca ccttgcgcac tggcaagatg ggcctgataa gtatggacag cggctttcgg
    26521 caaaccggta gaacccgagg tcaacgtcat tgagcacaga cgcgtcggct gccacgcagc
    26581 ggcatgtacg ccttcaacca gctgaatgtg cagcgacgtt aatgccggaa acgtgttttc
    26641 cccatccggc accagcgcaa attgcagcgt cagattgggc agcaattctt caagcaacgg
    26701 ttgcggcagc tgagggttca cgggcaacac ccgcgccccg cattgcagta acgccagcca
    26761 ggcgagcagc gtttgcggcg tattccacgc ccgcaacatc acgccgctgc cctcaaccac
    26821 cccctgcgcc gcaaagcccg tagctaactc atcgacgcga gcacaaagct cgcgccagtt
    26881 gagttgctcg tcattaagac gtaaggcgat ggcttctccc cgcacttgcc gccagtgacg
    26941 ccacggccag tcagagaaga tcataacaac tgctcctgta catcagcgtc cactaacggc
    27001 agtgggctcc ccggccagcg acgaacctgc tgcgcctgca tcagatccag cgtgtccagt
    27061 cccggaatgg tgtctggcgt taaccaggca gcaatccgcg ccagttgcgt tagacctaag
    27121 ctcgactcaa tggaagaact gatcaccgcc gtcagcccca gcgcatgcgc cgcctgtacc
    27181 tgctcgcgta ctttttccag actgcccgtg agcgtgggtt tgataactac cgcgcgcacg
    27241 ccctcttcag ccacaaaggc aaaatccggc tcgcgcaggc tttcatccca ggcaatggca
    27301 atgccggttt cacgggcaaa cgctcgcgaa tcatcgcggg ttttgcacgg ctcttcgaga
    27361 aacgcgatgc ggtggcgata atccgggtta acatatttgg caaactgctg acctttcagc
    27421 ggtgtccagg cgcgatttgc gtcaagacgc aaatgcagat ccggaattgc ctccagcaac
    27481 agattcacca ccatgccgtc gcgcaccgct tcgtacaatc ccactttgac cttcgccact
    27541 ttctcgcccg gcatatcggc aagtttgagg atcagatcgt ccggatcgcc attacacagc
    27601 ggtgccgcac ggtagttggc tgcttgcggc aacgtatctg tcagttctgc caatgcacag
    27661 cttacgccaa aggccacgga aggcatctgc ggtagctcgc aatcgcctgc cagccagtta
    27721 tttacccagg caagcagcac gctttgcgcc tcttcccagt tttcctggct gaagcccggc
    27781 agtggggaga tctcccccca cccttcgcgc tcgccctcac gcaggcaaac gtacagcccg
    27841 tcgcgggttt ttaaccgcct gtcgcgcaga accacccccg cgtccatggg gatctgccag
    27901 cggtatacct gcgcgctacg cattacggat tccgtttaaa tttgctgaaa tcaggctgac
    27961 gtttctggtt gaaagcgttg cgaccttcct gaccttcttc cgtcatgtag aacagcatgg
    28021 tggcgttgcc cgccagctcc tgcagccccg cctgcccgtc acagtcggcg ttcagcgcag
    28081 ctttcaggca gcgcagcgcc atcgggctgt tttgcagcat ttcgcggcac caacggacgg
    28141 tttctttttc cagatccgcc agcggtacca cggtgttgac aaggcccata tccagcgcct
    28201 gttttgcgtc gtactgacgg cacaggaacc agatttctcg cgcttttttc tgcccgacga
    28261 tacgagccat gtaggaagcg ccccagccgc cgtcgaagga accgactttc gggccagtct
    28321 gaccgaagat ggcattatct gctgcgatag tcaggtcgca catcatgtgc agaacgtgac
    28381 caccgccgat ggagtagcca gccaccatcg cgacaaccgg tttcggacag gtacggatct
    28441 gacgctggaa gtccagcacg ttcaggtgat gtacgccgga atcatcttta tagccgccgt
    28501 aatcaccacg cactttctgg tcaccaccgg agcagaacgc tttatcgcct gcgccagtca
    28561 gaatgatcac gccgatattg tcgtcataac gcgcatctgc cagtgcctgg atcatctctt
    28621 ttaccgtcag aggacggaag gcattgcgca cctgcggacg attaatggtg atttttgcga
    28681 taccgtcggt ggatttttca taacgaatgt cctcgaaacc ttcggagcag tcgtgccatt
    28741 caaccggtgc gtaaggcatt gcttcatcag gataaatcat attgtgtcct ttagtcagaa
    28801 acgcaagatc tgcgccagac ttgcgattac gccagcggga ttttcccgat gcgcgttatg
    28861 tccggcgcga ggaatgacat ggcagtcggc agccagttcc gccgccaggg cgcggaattt
    28921 gctgtcacgt tcaccacata aataataaaa cgcaaatgtg cgggcgctaa ggttagcacg
    28981 taaatcaggc tggacggcga gagaagtcgc ctccagcatg gcggcaaggg ttgcgccatt
    29041 attgttgctg cgcagcgcca ccagctcccg gcgttgatcg tcattgagcg aggcaaaaac
    29101 aggctgttga taccagtcgg caaataccgc cgttaacggt tctgtgcgaa aacgctgcgc
    29161 ccattggcga tcggaacgct gacgttccgc acgttgttca gcattttgca gccccggatg
    29221 cccgccttcg acaataaccc cacaaagccc cgccagcccc tggcaagccg ccatcatcgc
    29281 cacccgtccg ccaagcgagt accccaccag ccagaagtca aggatgttgt aactaaccaa
    29341 ggttttacgc agtaagtcgg tgacatcatc aaatccatcg acgctaatcg ccgccgaacc
    29401 accatgacct aggagatcaa catacaaccg tgagtagtcg gcaaacgcct cgcccacttc
    29461 ttgccattcg tggcaatcgc cggaaaaacc gtgcaaaaac accagccagg gtaaacctag
    29521 ttttccgtgt tttgcctgcg cgtgcaggat cataaatggc ttacctgcgc cagaagttgc
    29581 tggagcgttt gcgcaccatc ggtgtcgtta accaccattt caatcaccgt ggtggttggc
    29641 gtgcgccagg cgtcggcaaa tgccgtttca agttcctgcc agttttgcgg atgatgatat
    29701 ttcagctcga acatcgcggc ggcgtgctca aaatggacgt tttgcggcat cagatagaaa
    29761 cgctcacgct cgctttgcgg cgttggcaac agcgagaaaa tttgcccgcc gttgttgttc
    29821 accacaatta ataccagcgg cgcagaaacc tgacgcaata acgccagcgc gttgagatcg
    29881 taaagcgcgg agagatcgcc cacaatcgcc agcgtcggtt tgccgcttgc ccgctgaacg
    29941 cctgcggcgg tcgaaagcag tccgtcgata ccgctggccc ctcggttgct gtacaccggg
    30001 taacctgccg gaagttgcga aagcgcatca atcagacgta ccaccaggct gttaccaaca
    30061 aacaattgcc cctgttcagg cagatagtcg ctgatgcgat gcgccagttg cgcttcgcca
    30121 aacgcatcac ggcgggcaat aaccgcctgc attgcctgtt ccgccaggcg cgggatttca
    30181 acgcaccagg gctggcgttt ttctgccgga tgcagctcca gccagtcggc aatattggca
    30241 attaagcgac gtccgcgatg gtgtgccgga tcaagtcgcc cttcaatgtc atcaacaatc
    30301 cagtactctt ctggttcaca gcttgcctgc cattgcagga gccgtttccc cgtcaggcta
    30361 cttcccagtt gcaccacaat ttgcgcctgc tgcagctcgc tggtcgcttt ggcattgcct
    30421 aaccaaagat cggcacacgg cagcggctgc ccggtttgtg acagcacatc gccaatcagc
    30481 ggccagccaa gagtttgcgc ccacagggca acttttttgc cctcttccgc actcatgcgc
    30541 ccggcaacca ccacgccgcg cttttgtcgc cagaagaacc agtcgcgctg tttttcactt
    30601 tccagacgag gcgcttcacg cagccacggt ttgtcgtcct gccaccagtc acccagacgc
    30661 tgttgccagc taagcccggt atcgtccatt tcgccataca gcggttcagc aaacgggcag
    30721 ttgatatgga ctccccccgc atgaagcgta ccgagagcgt ggtcgatggt agaaaccagc
    30781 caacgtgcgg ggatatcctg ggtcgggcgc ggcaatgaaa tactgtgcgt ggggtgagag
    30841 gcgaacattc ccggctggcg aattgcctga ttcgcgccgc agtcaattag ctccggcggg
    30901 cgatcggcgg ttaagagaat cagtttttct ccggttaacc cggcttcaat cagtgccgga
    30961 tagagatttg ccaccgccgt gccggaggtc acaatcaccg ccaccggctg cttgctgact
    31021 ttcgccagcc ccagcgccag atgccccaac ccacgctcat cgaaatgggt gtggtgaatg
    31081 aatgcggaat tctccgccgc cgctaacgtt aacggtgtag aacgcgagcc tggggcgata
    31141 cagatgtgtc tgacgccgtg acgcgttaat gcttccagaa tgaccgccgc ccagcgtcgg
    31201 ttaaatgcgc ttactgacat gagtttgtcc ggtatcaata ttgcggctaa gtataaggag
    31261 ctacaaaaat agattattga tatgaatcgg taatgatgcg actcattact attccatttg
    31321 taataaagta cgcagccctg ccgctttgtt gtcgatttcc tgccactctt gctcggggtc
    31381 ggaaccacgg acaatgcccg cgccagcata taatcgcacg acattgccgc taatttttgc
    31441 tgagcgcagg gaaacgcaga attcgctttg ttgtaatgag agatagcccg cagaaccggc
    31501 gtaccattct cgggtgaacg gttcgtgacg ggcgataaac tgtcgcgcca gatcgcgcgg
    31561 taagccagca actgctgccg ttggctgcaa ctgatgtaaa cagatcacat catccgcttt
    31621 gttgagtgaa gtccagatac agcggcgaag atgctgcact ttacgcagac gcagtacctg
    31681 cggcggtaaa acatccagcg tctgggtatc ggcctgtaat cgttggcaga tatcttccac
    31741 caccagcatg ttctcgcgct ggtttttatc atccgccatc agccactctc ctaactgctg
    31801 cgcctgctta tcatcaggat gatttgctac tgttcccgcc agcgcttcag tacgcagcgc
    31861 tttgtcacgc cgccgccata accgttccgg tgaagagcca agaaaagcat tttcgccatc
    31921 aaaggccatg taaaaatggt agcaattcag attcagtcga cgactggcag ccatcatcgc
    31981 cgccgcgttg accggacttg cgaaatgcag gtcagttgcc cgagcgagca ccactttgtc
    32041 gagctcacct tcggcgatgg ttttcgttgc cagttcgatt aattgcgtcc agcccgtttt
    32101 gtccggccag tgttgttctc gcgtggtggt taaatgtaac ccaggcaagg gcttgatact
    32161 caccagtgtg gcgataaatt cttttgcctg aatcgcatcg tgctgaaggg agctttcgct
    32221 gaatagcgtc agccgcagcg tggctttacc gccacagcgt cgccattcca ggcggggtaa
    32281 aagtaaattg ccctgcgacg ggtcaaaagc attcagcccc caaatgcgta agtcagcgtg
    32341 ttccgggtgc tggcgaagaa aacgttgtgc ctggtccaac gacgtaaaac gggtaatcgc
    32401 gcccaggacg gcagcttctt catcaccatt acgttgttgc cagtagaatt gcgggtacgt
    32461 ttgctgactg gccagccagc tcaaggcatc aaaagcgtct ttgagaggga aaggaatatc
    32521 gataacccga atgccgggtg tcgccggaat ctcttgcgac aaatggcgca gtagattttc
    32581 cagcgccgta gtaagtgatt gcacgcggac ctctccctgt taaaagcctc acattatacg
    32641 gggtactaca aaaaaaatgc agtaccccgg tgtagggagg ttttaacggc gtgccagcaa
    32701 cagtcctagt accagcccga cggccgcgcc cacaccaatt ccttgccagg gtttttcgtg
    32761 gacgtagtca tcagcacgat aaacagcctg cttcgcccga tagtaataac tgtctgaagc
    32821 ctggctaacc cgttttttca catcatccag cgctttttct gcatgcgctt tcagctcaac
    32881 atatttctga tcggcgggat cgccagagga gcggagcacc tcttccagtg tttcactaag
    32941 cagcgtcagg tcgtcatcga tacgtgtatc accaaactga ttagacattc tcgttctcca
    33001 tttgcgtaaa acctgtcact aactatagac aagagttgtt gattacgcct gaattacctc
    33061 gcgtgccatc ccaatgtgtg ggataccatc ttcttcatag acctccgtca cagggataaa
    33121 accgaaactc tggtagaagt tttgcagatg cgcctgcgcc ccgaggtaaa caggcttatc
    33181 aggccagtga tgcgtacatg tttcgagtgt tttactcatc agctgctgcc ccactttttc
    33241 accacgcaac gcttcactga caatcacccg acctataacg accggctcaa gatcatcatc
    33301 acttttcaga atcctcgcat acgccaccag ttcatcattt ttccaaccga gaatatggcg
    33361 attatccccc gtcaaatcat caccatcgat atcctgataa gggcaattct gctcgacaac
    33421 aaacaccgcg caacgcagtt gtaataaggc ataaagctgg gaaaccgata attcagagtg
    33481 atgcagatct tgccattcaa tcataatgct ctccttttag atgtatgcct tatactagcc
    33541 ggaatatttt ttgaaacgtg atgaactcat ggaattaatt tttttaggta cttcagccgg
    33601 tgtgccaacc cgcacgcgca atgtcacggc aatattgctt aacctgcaac atccgaccca
    33661 gagcggactt tggttgtttg actgcggtga aggcacccag catcagctac tgcataccgc
    33721 ctttaaccct ggaaaactgg acaagatttt tatcagtcac cttcatggcg atcatctttt
    33781 tggtttaccc ggcttgctgt gcagtcgttc tatgtcaggc attatccaac ccttaacgat
    33841 ttatggtccg caaggtatcc gtgaatttgt ggaaaccgcg ctgcggatta gcggctcatg
    33901 gaccgattat ccgctggaaa ttgtcgaaat tggcgctggc gaaattctcg atgatggcct
    33961 gcgcaaagta accgcttatc cgctggaaca cccactggaa tgttatggct atcgtattga
    34021 agaacatgat aaaccgggtg cattaaatgc ccaggcatta aaagctgctg gcgtgccgcc
    34081 tggcccactg tttcaggaat taaaagcggg caaaacaatc acgctggaag atggaaggca
    34141 gattaacggc gcagattact tagctgctcc agtgccaggt aaagcgctcg ctattttcgg
    34201 cgataccggc ccctgcgatg ccgcacttga cctggctaaa ggtgtcgatg tcatggtgca
    34261 cgaagcgacg ctggatataa ccatggaagc caaagccaat agtcgcggcc atagctctac
    34321 acgccaggct gcgacactag cccgtgaggc tggagtcggc aagctaatca ttacccacgt
    34381 cagctcgcgc tatgatgaca aaggttgtca acacctgtta cgtgaatgca ggtcaatttt
    34441 cccggcgact gaactggcga atgatttcac cgtgtttaac gtttaactct gcttattatt
    34501 aaaaacaggg tgaaacttgc cctgttatag caacccgcgc tacatatatc cacccacaca
    34561 ggcatattga gcgatttttg atatttactc atctccttac gcactatatt cagagcaata
    34621 agttgcctct tttcatcttg attcagcaca acctttgcaa ctaaaaggag cccatgatgg
    34681 ttacagttgt cagcaattat tgtcaattat ctcaaaccca actcagtcag acatttgcag
    34741 aaaaatttac tgtgaccgag gaattactgc agtctttaaa aaaaacagcg ttatccggag
    34801 atgaagaaag tattgagtta ctgcataata ttgcgttagg ttatgatgaa tttggaaaaa
    34861 aagctgggta atgctgccaa cttactgatt tagtg
//
LOCUS       DAJTOW010000033        34887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTOW010000033 DAJTOW010000000
VERSION     DAJTOW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.033"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(54..129)
                     /locus_tag="OO244_001721"
     tRNA            complement(54..129)
                     /locus_tag="OO244_001721"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:His,seq:gtg)
     gene            complement(188..264)
                     /locus_tag="OO244_001722"
     tRNA            complement(188..264)
                     /locus_tag="OO244_001722"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Arg,seq:ccg)
     gene            complement(367..1752)
                     /locus_tag="OO244_001723"
     CDS             complement(367..1752)
                     /locus_tag="OO244_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5663580.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1943..2683)
                     /gene="rffM"
                     /locus_tag="OO244_001724"
     CDS             complement(1943..2683)
                     /gene="rffM"
                     /locus_tag="OO244_001724"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5663581.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2686..4038)
                     /gene="wzyE"
                     /locus_tag="OO244_001725"
     CDS             complement(2686..4038)
                     /gene="wzyE"
                     /locus_tag="OO244_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5663582.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4035..5114)
                     /gene="rffT"
                     /locus_tag="OO244_001726"
                     /gene_synonym="wecF"
     CDS             complement(4035..5114)
                     /gene="rffT"
                     /locus_tag="OO244_001726"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5663583.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5111..6361)
                     /gene="wzxE"
                     /locus_tag="OO244_001727"
     CDS             complement(5111..6361)
                     /gene="wzxE"
                     /locus_tag="OO244_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5663584.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6363..7493)
                     /gene="rffA"
                     /locus_tag="OO244_001728"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6363..7493)
                     /gene="rffA"
                     /locus_tag="OO244_001728"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5663585.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLAQWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7498..8172)
                     /gene="rffC"
                     /locus_tag="OO244_001729"
                     /gene_synonym="wecD"
     CDS             complement(7498..8172)
                     /gene="rffC"
                     /locus_tag="OO244_001729"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5663586.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8150..9031)
                     /gene="rfbA"
                     /locus_tag="OO244_001730"
     CDS             complement(8150..9031)
                     /gene="rfbA"
                     /locus_tag="OO244_001730"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5663587.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9050..10117)
                     /gene="rffG"
                     /locus_tag="OO244_001731"
     CDS             complement(9050..10117)
                     /gene="rffG"
                     /locus_tag="OO244_001731"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5663588.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10117..11379)
                     /gene="wecC"
                     /locus_tag="OO244_001732"
     CDS             complement(10117..11379)
                     /gene="wecC"
                     /locus_tag="OO244_001732"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5663589.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11376..12506)
                     /gene="wecB"
                     /locus_tag="OO244_001733"
     CDS             complement(11376..12506)
                     /gene="wecB"
                     /locus_tag="OO244_001733"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5663590.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12562..13608)
                     /gene="wzzE"
                     /locus_tag="OO244_001734"
     CDS             complement(12562..13608)
                     /gene="wzzE"
                     /locus_tag="OO244_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5663591.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13620..14723)
                     /gene="wecA"
                     /locus_tag="OO244_001735"
     CDS             complement(13620..14723)
                     /gene="wecA"
                     /locus_tag="OO244_001735"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5663592.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14963..16222)
                     /gene="rho"
                     /locus_tag="OO244_001736"
     CDS             complement(14963..16222)
                     /gene="rho"
                     /locus_tag="OO244_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5663593.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16307..16408)
                     /gene="rhoL"
                     /locus_tag="OO244_001737"
     CDS             complement(16307..16408)
                     /gene="rhoL"
                     /locus_tag="OO244_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5663594.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16549..16878)
                     /gene="trxA"
                     /locus_tag="OO244_001738"
     CDS             complement(16549..16878)
                     /gene="trxA"
                     /locus_tag="OO244_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5663595.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            17009..18274
                     /gene="rhlB"
                     /locus_tag="OO244_001739"
     CDS             17009..18274
                     /gene="rhlB"
                     /locus_tag="OO244_001739"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5663596.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18408..19892
                     /gene="gppA"
                     /locus_tag="OO244_001740"
     CDS             18408..19892
                     /gene="gppA"
                     /locus_tag="OO244_001740"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5663597.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19939..21960)
                     /gene="rep"
                     /locus_tag="OO244_001741"
     CDS             complement(19939..21960)
                     /gene="rep"
                     /locus_tag="OO244_001741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5663598.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22047..22328
                     /gene="ppiC"
                     /locus_tag="OO244_001742"
     CDS             22047..22328
                     /gene="ppiC"
                     /locus_tag="OO244_001742"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5663599.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22374..23849)
                     /gene="ilvC"
                     /locus_tag="OO244_001743"
     CDS             complement(22374..23849)
                     /gene="ilvC"
                     /locus_tag="OO244_001743"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5663600.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23999..24889
                     /gene="ilvY"
                     /locus_tag="OO244_001744"
     CDS             23999..24889
                     /gene="ilvY"
                     /locus_tag="OO244_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5663601.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24886..26430)
                     /gene="ilvA"
                     /locus_tag="OO244_001745"
     CDS             complement(24886..26430)
                     /gene="ilvA"
                     /locus_tag="OO244_001745"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5663602.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26433..28283)
                     /gene="ilvD"
                     /locus_tag="OO244_001746"
     CDS             complement(26433..28283)
                     /gene="ilvD"
                     /locus_tag="OO244_001746"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5663603.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28348..29277)
                     /gene="ilvE"
                     /locus_tag="OO244_001747"
     CDS             complement(28348..29277)
                     /gene="ilvE"
                     /locus_tag="OO244_001747"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5663604.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29297..29560)
                     /gene="ilvM"
                     /locus_tag="OO244_001748"
     CDS             complement(29297..29560)
                     /gene="ilvM"
                     /locus_tag="OO244_001748"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5663605.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29557..31203)
                     /locus_tag="OO244_001749"
     CDS             complement(29557..31203)
                     /locus_tag="OO244_001749"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5663606.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31206..31256)
                     /locus_tag="OO244_001750"
     CDS             complement(31206..31256)
                     /locus_tag="OO244_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663607.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31342..31440)
                     /gene="ilvL"
                     /locus_tag="OO244_001751"
     CDS             complement(31342..31440)
                     /gene="ilvL"
                     /locus_tag="OO244_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5663608.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31792..33271
                     /locus_tag="OO244_001752"
                     /pseudo
     CDS             31792..33271
                     /locus_tag="OO244_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="OO244_001753"
     CDS             complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="OO244_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5663609.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33753..34592
                     /gene="hdfR"
                     /locus_tag="OO244_001754"
     CDS             33753..34592
                     /gene="hdfR"
                     /locus_tag="OO244_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5663610.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34688..34763)
                     /locus_tag="OO244_001755"
     tRNA            complement(34688..34763)
                     /locus_tag="OO244_001755"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34728..34730),aa:Trp,seq:cca)
     gene            complement(34772..34848)
                     /locus_tag="OO244_001756"
     tRNA            complement(34772..34848)
                     /locus_tag="OO244_001756"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34812..34814),aa:Asp,seq:gtc)
BASE COUNT         8506 a         9551 c         8808 g         8022 t
ORIGIN      
        1 agctagcgcg tctaccaatt ccgccacctt cgcattgtca caacttctaa taatggggtg
       61 gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta ccaactgagc
      121 tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct caagcgctgg
      181 gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg agggcagcgc
      241 tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat acggacttcc
      301 cacccctctg tctagtccct ttttaaataa attgctcgtt tggttacggt ttgtgcgttt
      361 tgctgcttat tcctccagtt tatgcgcttt accgtggcga ttaagcccaa aaactttata
      421 aatcgccgtc accgccagca taaagatgat gccgacaaac agcgacatac gcgtatcttc
      481 attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt agttggccca
      541 cgggaacagg attgagcgga acggatggct ggcaatcgcc gctttatgtg cacgccgaaa
      601 acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca cgctggcact
      661 gtagacgtag acaaacacac gctgcggatt gggaatgatg tagttcaggc atgagccaat
      721 tagcagaata gcaatagata ctgccacacc cgcaaccggt acgccgtgac gggaaacttt
      781 tgccattgcc gccggtaact gacggttttt cgccagtgcg tagagcatac gtccgcaact
      841 gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt tgataatgcc
      901 cgccgctgcg gtaataccga ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc
      961 aatttcattc cacgggaaga tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag
     1021 gatccgccac agcactttgc ctacggcact gcgcagcgtc acctgcggat tcttcgcttc
     1081 accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa tacacagagc
     1141 ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa
     1201 accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccaggc caatgacaat
     1261 catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt cgccgtacaa
     1321 ccgtaccgcc gccagattcg ccaacgccac cagcgccacc gcgatcaatg cgggtatcca
     1381 ctgcgccatc tccggggacc aaaactggac ataaacgcca atggcggtga tttctgagat
     1441 ccccaccgcc atccacataa accagtaaga ccaggcggtg agatagccaa aaaacgggct
     1501 catataacga tgcgcataaa cggcgaacga accggtaacc ggttcgagga acaacatttc
     1561 gcccattgaa cgcatgatga aaaaaacgaa cagcccggcg atgatatagg ccaacaatac
     1621 ggatggcccg gcccatttca gggtactggc ggcccccata aacaggccga cgccaatggt
     1681 gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta gctctggttt
     1741 gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt
     1801 tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc aaataaagca
     1861 ttaaaaattt gaatgctttg tgtaataaaa aagcagacag gcgacgaagt gaccactccg
     1921 tcgctttaca aagagaggaa aatcagaggt tgccggtgta gtgccagcgt aaataacgca
     1981 gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag agccactcca
     2041 gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag
     2101 tcccgccaac gcccatatac agcgcatctg gatgcaccag acggcagtcg cgcatgaaga
     2161 tctcctgctt tggcgatccc atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc
     2221 gttcaaacag cgcctgacgc tgctcgggtt taaaataacc atcctgactg ccaacgatat
     2281 tcacattcca ttggttgcgc agtttagctt cagtttgcgc cagcacttca ggtttaccgc
     2341 ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc catcagctct tcccagagat
     2401 cggcaccggc aacgcgggaa acctgcgcct gcgggtactt tttacgtact gaacgtacaa
     2461 tgctgatgcc atccgcatat ttaaattcgg cagcgttaat taactccctg acctcggcgt
     2521 tatcttcaat ggtcagcatt ttttcggcat taatggcaac cagcgttccc tgcttaagct
     2581 gcccgtcagc aaacagataa tcgagggcat gctgcatatc acgccaacca atcaactgta
     2641 agccacgcag cgtataggtc ggtgccgtgg tgttgttatt cattgttatc cttcaacctg
     2701 cgtccggagc gatgattttg tacgtttatg aatgagtccg gcgctttcaa aaagccagta
     2761 caacagtttt gcgatcatca gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga
     2821 gacaaacgaa tccagccctt cacgtgccag cacgatcata ttgaagatcg ccccaaagca
     2881 gaaactgtgc aatatcgcag ccttatagcg attagtctcg cggttgccca gctcatacag
     2941 ccagtcgaac catttgatga tcagtccaac cacaatcgcc ccgagtggga tgaacaacgc
     3001 gccgcccatc accaccagtg agcctataag cgtaggcgag atcgccagtc cggagtggtt
     3061 attcagtact tcccaggtaa agtaattggc tgagttcagc accatactcg ggcgccccgg
     3121 ccacagccag gaagggataa agacatagaa atcgcggaca attggagcca ggccctggaa
     3181 gtcgatgttg tcgtagttct gcaacagcaa cgccagattc tcccacggtg aaaaggtgtc
     3241 gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg ctcacattca ttccatagcg
     3301 ttttaacgcc agccagaaca tgccaacaat ccccagcacg cccgccgccg ccagcatcca
     3361 caacgaaatc cagccgcgaa taatgccaat aaacaggaag atagcgaatg cgatgatgat
     3421 attggcgcga gtgccgccga caatcatata agtcagcaag ccaaaggcga ccgtgctgac
     3481 gaggaaaaac agccacgctt tgctgtcctg gcgcagaaag tagaccacca gcatcgccgg
     3541 gatgaaaaag taaaagaagc gttttaacgc cacgccggag acttcactgg aaaagatctg
     3601 actgtaggag ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct
     3661 taccagcgcg atacccatca ggatcaccca cgtaagattg gtctccacgc ggttcatggt
     3721 aaacagcgga cggcgcggta catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac
     3781 atagtaaacc gcgtagaagc agcccgcaga aagcaacacc tgcaacaaga tttctggcgg
     3841 cgcgacacca acatcaaagc gaaataccag cacgctggtc agcgggaagc cgaagaaaaa
     3901 ggtgagcaaa aacagcaatg aaaagaagac attgaagtta aagcgcacac ggcgaaactc
     3961 aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc cagaccacaa acaggccact
     4021 gaattgcagc agactcatgc gacctccccg gcggcaatcg ccaacgcccg ctgccagcct
     4081 tgtagatagt tagggctaaa gaaggcaatg gtgtttttat ccgccgacgc caactggcgc
     4141 tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag taaacagcac tggcaaatgc
     4201 tgttccgtca tatcctgcca gaacggattt tccctgttaa gcacacaagg aatgcccgcc
     4261 tgaatcagta agcaaagcgt accaatgccc tgctggcggg caaaaataaa gtaaccaaga
     4321 tcgcactgac gaagtagcgc cagataggcg tcaaattcca gtttttcgct caaaatttgc
     4381 agattttctt cgctgaataa ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg
     4441 ttattaggcg gatatcccat cggcaccacc acttttaccg tatcgccaaa ttgctgatga
     4501 acggcacgta aagcggcgac atgctcattg ctgcggtcgc cggagttccc caccagaatg
     4561 gtcatttttc cttcacgttg ccgatcgttc gccatcgtat tgagagaagg gtccatccgc
     4621 gtcgggaagt acagcagttc tccccgcacc tttgggtgtg ttttggcaaa aaagctcaaa
     4681 tcgccgcggg tggcaaatac acagccgact cgcttttgcg ccaggcgacg tagtgggtaa
     4741 aaaagcttat atctcaagcc actggaaagc tcatacaggt ctgcccccca gatatgccag
     4801 aaaaactggc tgggcttaat gccaccactc agcagagcca gccacagtgt gggattgaac
     4861 tgaccgtgga aaaaaaatcg ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc
     4921 gccagcgatt ttttcccagg gaaaaattgc acagaaagcg ccggacagct atcacttaag
     4981 ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc ggccagcgcg
     5041 tcattgaaaa accgcaaaac ggttcggtta tggtgaggga tatccgatcc cagtacgtga
     5101 atcagtacag tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa agagaaaaat
     5161 agacgatata agttgccata tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta
     5221 gccagtgggc aaataccatc aataaagtga actggctgac ttccgccaga atataaaacc
     5281 gcagtgacgc tttggcgatc accagataac caaagacata agcgcccact tttaacacat
     5341 cacccactaa ctgccaggca aagagatcgc gcatagcggt aaatttattc gacaacagca
     5401 accagatagc aaaatcacgc agcaaccaga cggtaaaact cgctgccgcc actgccggta
     5461 agacgaattt cagcgattta accacttccc gggtgatatc gcgcttttcc gttagccgcg
     5521 acaacgtggg cagcaaatat acgctgaacg atgccgtaat aaattgcagg taggcatcgg
     5581 aaatactgct caccccttgc cagatcccca cttcatccca gctatactgc gccgccagca
     5641 gtttacgcat catgatgtaa gcaacaggca aggtcaccga cgtaatcaac gccatgagcg
     5701 taaatttgct caactgccct gccagaccgt tatcccagct gggtttcaga tagcttaacg
     5761 ggatgacacc acgtttaatc aacatgatgg cggcaggaat taccaccagc gcgggaatca
     5821 gcgccagacc cagcaacgcc ccttcataac cgcccaaacg gtaactgacg tagtacgcga
     5881 gaacgccaat caagctgccg acaatcagcg ataacgcatt acctgcggca tcgcgaaagc
     5941 ctttcatcag cgccagtaac aggttgcccc aggcgatccc catttgcacc agcgccacta
     6001 aacgcaccag cccctgatag tcggtattac caaacaatcc ctggctgatt ggcgcagctg
     6061 ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag taccatcgct gatgaagtgc
     6121 cgaccacgcg gcgcagctgt tgcggattat catggtactg ggcaacgtat ttggttacac
     6181 cgttaaagat gccagccccg gcaagcacgc cgagcacggt aatcaactgg cggaaatttg
     6241 ccgccagccc aagccccgcc ggaccaaatg acaccgccag caacttaccg accagtaacc
     6301 cggcaccaat cttgaccagt gtactggccg ccgtccacaa ggacgctttt gccaacgaca
     6361 tatcaggaga agtagttcaa caaagtcgca attaccgtac gctgattgac gggcgacagg
     6421 ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt tggtggtgta gcgatcttca
     6481 ccgtggaact caccaaagtg ttcccccgca gggcaaccgt gcagcggaat gtaatgaaac
     6541 accgccatga tttccgcttc tttcagaaag ttaatcaacg cgctccggtc atcaatatcc
     6601 cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc catcgggaat cgacggcagc
     6661 tcgatacgcc cggctttcgc cagaggcgct aacgtatcgt agtagttttg ccactgcgcc
     6721 agacgttgct ggttgatacg atccgctgct tccagttgcg cccacaggta cgcagcttgc
     6781 agatcggaca tcaaatagct ggagccaata tcgcgccagg tatatttatc gacctgacca
     6841 cggaagaact ggctgcggtt agtgcccttt tcacggatga tctcggctcg ttcgattaac
     6901 gctttatcgt taatcagcgt cgcgccgcct tcaccacccg ccgtgtagtt tttggtttca
     6961 tggaagctaa agcagccaat atgaccaatg gttcccagtg cacgcccttt gtaagtggac
     7021 atcacgccct gagcggcatc ttccaccaca aacaaattat gctttttcgc caacgccata
     7081 atggtgtcca tttcgcaggc cacacccgcg taatggaccg gcacgataac gcgcgttttg
     7141 tcggtgatcg ctgcttcaat caacgtttcg tcgatgttca tggtgtccgg gcgaacatcc
     7201 acaaagacga ttttcgcgcc acgcagcaca aaggcattgg cggtggagac aaaggtgtag
     7261 ctcggcatga tcacttcatc gccaggctgg atatcgagca gcagcgccgc catctccagc
     7321 gaagcggtgc aggacggcgt cagtaacact ttggcgctgc caaaacgttg ctccagccac
     7381 tgctggcagc gacgggtaaa accgccatcg ccacacagtt tgccgctacc cattgccgac
     7441 tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt taaatggaat catgtgatca
     7501 cctgtataac cagtacgcgg tgctttctac attcgcacca ctttgtatgt atcgtttaag
     7561 cgcggcggtg ttgcccattt gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc
     7621 ccagtttagc gccgtttgca tcagctcagc acctgcaccg cgtccagcca gcaggccaat
     7681 tcgcgcatct gtcgcattga gttcccgtaa agagacatag ccgcgaatat cgccggacgc
     7741 cgcacgtaga atcagacatt gatgatcaaa ggtgccgcgc acggcatttt caatccactg
     7801 tgcataaaag cgaccgctgg cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc
     7861 aaatgcggcg ctagctaact gacgtaatgc gggaatatcg gtctcttgtg ccacgacagc
     7921 accgctatca ctgacattgt tcacgggtag cgccaaatca acttcacctt ctaccaggga
     7981 gaatcccagc tgttgcaggg catccagttc ccccgtattt gatgccgcaa ttttggcctg
     8041 cacccgtgac catggcgcta aggcgtctga cgtcaggagc ggtgcttcag acgtaatgcg
     8101 cacgatggcg ctgttaacac caaagaaggc gttttcccag gttagtggct caatactggc
     8161 gcggacgggc acgaagtaac tccagcagat attggccgta gccagttttc gctaatgaac
     8221 tggcagcacg cttcacaccc tcgtcatcga gccagccgtt acgccaggcg atctcttcca
     8281 ggcaggcaat cttaaagccc tggcgttttt ctactgtctg cacaaaagtg ctggcctcaa
     8341 tcaggctgtc gtgagtgccg gtatccagcc aggcaaatcc gcgcccgagc agttcaacgg
     8401 tcaggttgcc cgcctcgagg tacatctggt tgatggaggt aatctccagt tcaccgcgct
     8461 ccgacggctt cacctgcttt gcgtactcca cgactttact gtcgtagaaa taaagcccgg
     8521 tcaccgccca gtttgacttc ggctgttttg gcttttcttc aagcgagata gcgcggaaat
     8581 tgtcgtcaaa ttccaccacg ccaaagcgtt ccgggtccat cacctgatag ccaaaaaccg
     8641 tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc tgaccgaaga
     8701 agatgttatc gcccagcacc agacaagaag gttcaccatt aaggaaggtt tcaccgatga
     8761 taaaggcctg cgccagaccg tccgggctgg gctgttcggc atattccagc tggataccga
     8821 actcactacc atcgcccagc aggcgctgga aataaccttt atcttccggc gtagtgatga
     8881 tgagaatttc gcggataccg gccagcatca gcaccgacag cggatagtaa atcattggct
     8941 tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc aatcgggtac
     9001 cggaaccgcc cgccaggata atacccttca tgtttgcctc cgctgaaaat tagcctttca
     9061 gacctaaacg ctcaccctga tagctgccgt cctgcacctg cttccaccag ctttcattag
     9121 ccagatacca ctgaaccgtt ttacgcattc cactttcaaa ggtttcctgc ggcagccagc
     9181 caagttcacg ggcaattttc gaagcatcaa tggcataacg cagatcatgc cccggacggt
     9241 cagcgacaaa ggtgatcagg tcacgatagt acgccacgcc gtgcggcttg ttcgaagcca
     9301 gttcttccag cagctcgcaa atggtttcca caacatcgag attcttacgc tcgttgtgac
     9361 caccaatatt ataggtttca ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg
     9421 cgtgatcttc cacatacagc cagtcacgga tttgctgccc gttgccatat accggcagcg
     9481 gtttacccgc cagcgcgttg aggatcatca gcgggatcag tttttccgga aagtggtaag
     9541 gaccgtagtt attcgagcag ttggtgatca gcgtcggcag accgtaggtc cgcagccagg
     9601 cgcgcaccag atggtcgctg ctggcttttg acgcggaata agggctgctc ggcgcatacg
     9661 gcgtggtttc ggtgaagaaa tcatccgtcg agtgcaggtc accatatact tcgtcggtgg
     9721 agatatgatg aaaacggaac gctgattttt tatcttccgt cagcgtattc cagtaagccc
     9781 gcgccgcttc aagcaatgta taagtcccga caatgttggt ttcaataaac gctgccgggc
     9841 cgtcaataga acggtcaaca tggctttcgg ctgccagatg catgacacag tctggctgat
     9901 gctcagtgaa tacgcgtgcc agttctgccc gatcgcagat atcaactttc tcaaaggcaa
     9961 agcgctcgct ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag gtcagcttat
    10021 cgaccactac caccgcatcg ctcgtttcgt tgatgatata acgcaccagc gccgagccaa
    10081 taaacccggc accacctgtt atcagaattt ttctcatcag cgccagactc ctttggcatc
    10141 gacgacatac tgctgatgga cattgtcgcc attgataact ttgaactgac tatgatcgac
    10201 cagcatcacc agcacgtctg ccgttgccag cgcctcgtca agctgcgcca gagtacaaag
    10261 cccggtcagt tttttcggca actggtggat gttaggctca acaaccagcg tttcgccgct
    10321 gtgccactgg gcgatcagtt cagcgatttc catcgccggg ctttcgcgca ggtcatcaat
    10381 attcggttta aacgccagac caaagcaggc gattttcagt tcactggctc gtttatcggt
    10441 agcagcaagg cagtcggcaa ccgccgcttt cacctgatcg ataacccaga acggtttgtg
    10501 atcgttaact tcgcgcgcgg tacggataag ccgcgcctgc tgggggttct gtgccacgat
    10561 aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca gggccaggct gaagaatatt
    10621 aacgcgaggg tgacgattcg ccaggcgaat cagttcccag acgttaatcc cctgatcggc
    10681 acaaatcagc gacaattcat tagcaaaagc gatattcaca tcgcggaagc tgttttcggt
    10741 gagcttacac atttccgccg tccgcgagtt agtgacgaca cactcacctt cgaggaaaat
    10801 tttgtacaat tcgctggcgc gggccgaaca aaccggcgtc ataccaccaa tcacgcgatc
    10861 gtttttaatc agctcgacca taacctggcc cggtaataca cgttccgggc agtaagcaat
    10921 gttgacgtcc gcctgctcgc ccacctgctg tgggaaagtc agatccgggc gcatctctgc
    10981 taaccattct gccatcttct cggttgaccc caccggcgag gtggattcaa ggatcaccag
    11041 cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc
    11101 tggctcatga tcgcctttaa acggcgtagg tacagcaatc agccaggcat ccgcttcaac
    11161 tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca gtttttacta cgctcgccaa
    11221 atcaggctcg acgatatgga tttcgccacg attgatggta tcaaccgcgt gttggttgat
    11281 atcgacacca atcacctgtt tttgccgtga ggcaaacgct gctgctgtag gcagcccgat
    11341 gtaacccagt ccgataacag aaatggtcgc aaaactcata gtgatatccg attatttttt
    11401 aacgcttcca gaatgcgaga gcatgcctga ccatcaccat acgggttatg ggcgcggctc
    11461 atagcttgat attcgttttc gtcttttaaa agacgcgtca cttcctcgac aattcgctgc
    11521 ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg cttccggacg ctcagtggta
    11581 tcgcgcatca ccagcaccgg tttccccagc gaaggcgctt cttcctgaat gccgcctgag
    11641 tcagtcaaaa tcagccaggc gtggttcatc agccagacaa acggtaaata ctcctgggga
    11701 tcgatcagaa tgacattttt cacatgcccc agaatgcgat tgaccggctc tctgacgttc
    11761 gggttgagat gcaccggata gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc
    11821 agcgcgtggc agatttcttc aaagccacga ccgaaactct cacgcctatg accggtcacc
    11881 agaatcatct ttttatcggg ggcgataaac gggtaatttg ccgccagttc tgaacgcagc
    11941 gtgtcgctgc tcatcacctg gtcacgcacc cataacagtg catcaatgac tgtattaccg
    12001 gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca agttttgccg ggaagtttcg
    12061 gttggagaga agtgatacat cgccagatgc ccggtcaatg tacggttagc ctcttccggc
    12121 cacggcgaat agagagcgcc cgtgcgcaga ccagcctcaa cgtgaccaac aggaatacgc
    12181 tgataaaacg ccgccaggct ggttgccagc gtcgtcgtcg tatcgccgtg aaccagcacg
    12241 acgtctggtt tgaactcggc aagaataggt tttagccctt ccagaatccg acaggttatc
    12301 tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac aatggaaaag
    12361 agtttcagta cctgatcaag catctcccga tgctgcgcag tgacgcaaac tttagcctca
    12421 aaaaaaggat cttttgccaa cgcatgcacc aacggagcca tcttgatggc ttccgggcgc
    12481 gtaccaaata cagtcagtac tttcacatcg attctcttcg aataagcggc gagcgccttt
    12541 gcgctcaccg cagcagtgtt gctatttcga gcaacggcgg gttaatgcga caccagcccc
    12601 gatcagcccc ccgacaatgc cccacataat catcaggaag gcacgacgtg ggctatcgcg
    12661 ttttaccggt tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg gatccagggt
    12721 tggaccaaca ttcagggtgt ttaacatggc tcgattctga tcatagtcga gatcaaaggc
    12781 cggaccgacg gcctgtaaat tttccagtcg agcctggagc attggacgcc caagcaggaa
    12841 catttctgaa tcaggtaatt cctcggcagg cacatctgtc gcactgcgcg aaatattatg
    12901 ctgctcagca attttcagcg cctgctcaat actgttcatc cggcggtcgt agatggcttt
    12961 cgccacctct tcctgacgct tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc
    13021 gcctttcagc tcatcattca gatggctggc tgcacgctgg ctggcaaaag caacatactg
    13081 acgtaacagg ttattagcgt caggcgcggt ttcggcaata agcttcacgc tgtcattgac
    13141 cgcgcgagta aagtctccgg ggataaactg gatgttgtta atcatttcat ccagcaacgc
    13201 cgcatcggct ttgctgttgc ccaccatccg ctgtttgtaa tagtcggttt gcagccagaa
    13261 ctctctgcgg gtatcccacg aggccagttg cataacaaac tctttatagg cttcgtccat
    13321 gaccgatggt tgatcggcag aagccatgtt tgaacggaca tccaggttac gcaaaaattg
    13381 ctgctgcgag taatatcccc ccagcatatt caccgttgga cgatcggtaa tcgccgtcga
    13441 gctccactcc tgacgagcaa aaaaagtata cgccagcgcg attaacgcaa acgccagccc
    13501 catgccaata atccatagct tcccagccca caaggtacga aacaacccac gaatatccag
    13561 ttcattttca gcgtcttcgg ccggtttccc aggcattggt tgtgtcatca catcctcatt
    13621 tatttggtta aattggggct gccaccacga tttctacgca gtctgcgttt tacgcgctta
    13681 ataaagcgag caactttcca ggcacgctta atgcaatatc catagaggaa gaatgctagc
    13741 aaaaagagca ccagcatgac ccactccggg acaaaatgag aatattctgc cagcacgcca
    13801 atggaagcga gcagtgctgc ggcaagggta atcagcacaa acgcctgacg ggaagtaaac
    13861 ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag gagagaatgg gctcatgcct
    13921 ttacgcaggc gacggtacat aatcgccacc atatccatta gcggaatggc gattatccac
    13981 aaagcggtaa ccgggctgat gggatgggtt ttgccctggg tcgtttcgag caggatccag
    14041 ataacggtaa aaccaatcag cgtactgccc gcatcaccca taaagacttt gtagcggcga
    14101 cccaggatac caaggttaag catgatgtat ggcaggatgg cggcgatcat cgcaaagcac
    14161 cagattgcga ggctggtttg cccgtcgaac cacaaaatca taccgattgc tgcaaacgag
    14221 acgcaggaca acccgcccag caagccatca atgccatcaa ccatgttgaa cgcattaatg
    14281 gccgcccaga cggcaaatag cgtcaggaag taaccaaacg gtccgagcac catctcccag
    14341 gagccaaaga tataacccag gctactgaga taaagtttgc cgaacaccat cataacaatg
    14401 ccaacagcgg cctgtatggt ggcacggatt tttacgctga tatcaaaacg atcatccagc
    14461 gcgccaatga aaacaagcac accggcacaa gcgagataga gagatgcatg cggaatatag
    14521 taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga
    14581 ggtatcaatc cctggtgacg tttgcggaag tttggtttat ccactaaacc gacttttttt
    14641 gccaccttac gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact gatgagatca
    14701 gtactcactg tcagtaaatt cacaatgcat gctctcagag aaaattatta gcagaagtat
    14761 aaccacgaag acctttattc agaagggaaa gtccgttccg aacccaccag ccctgtgcaa
    14821 aaatcaacca tttgatatgc aaattgcata acatgccagc aaattccagg aattttccta
    14881 acatcgccag cgcgacatta agattacaga tataaaagca aaacgccacg taaacacgtg
    14941 gcgtttttgg cataagacaa atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg
    15001 tcttggtcat ggccagttta ttaatgagga attccattgc atcgatttcg cccatcgggt
    15061 gaatgatttt gcgcaggatc cacattttct gcagttcttc ctgagtcgtg agcagctctt
    15121 ctttacgggt accggaacgg ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct
    15181 tacgagagag gtgcagttcc atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt
    15241 ccattttaga accggtatcg ataagcgccg tcgcgataat ggtcaggctg ccgccctctt
    15301 ccacgttacg cgccgcacca aagaagcgtt tcggacgatg aagggcattg gcgtccacac
    15361 caccggtcaa cactttacct gacgccggaa caacagtgtt gtaagcgcgc gccagacgag
    15421 tgatggagtc gagcagaatg ataacgtctt tcttgtgctc aaccaggcgt ttggccttct
    15481 cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa
    15541 caacttcacc ttttaccaga cgctgcatct cggttacttc ttccggacgt tcgtcgatca
    15601 gcagaaccat cagcacacaa tccgggtggt tgtaagcaat gctctgagca atgttctgca
    15661 gcagcatagt tttaccggct ttcggcggtg ccacaatcag accacgctga ccacgaccga
    15721 taggtgatgc cagatccagt acgcgagcag ttaaatcttc agtagaaccg ttaccacgtt
    15781 ccatacgcag acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc
    15841 gggcgttttc aggtttgtcg aagttaactt cgttaacttt cagcagcgca aaatagcgtt
    15901 caccttcttt cggcgggcga atcttaccag agatggtatc accagtgcgg aggttgaaac
    15961 ggcggatttg gctaggggaa acgtagatgt catcaggacc ggcgaggtag gagctgtctg
    16021 cggaacggag gaaaccaaat ccatcctgca atatctccag tacgccatca ccaaagatat
    16081 cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc ttacgcatgc
    16141 gagccaggtt ttccagcccc atattttcgc cgagagtgat cagctcagaa accggcgtat
    16201 tctttaattc ggtaagattc atagtggtgt gagttcttaa acttggggta aatctcgaac
    16261 ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt ttacggtcat cgactcatgt
    16321 cttttcgctg cctggtcaca ggagagtacg cagaactgaa acgacaagac ggattgagtg
    16381 acgagccaga aatctgttca cttcgcattt aagataaaac gggaagcgtt ggaaattaca
    16441 agattcaaac ttaataaggt atgtttaata cgaagtcaac actaagttag catgactcac
    16501 gccgggcgtc cagtttttag cgacggggca cccgaacatg aaattccctt acgccaggtt
    16561 agcgtcgagg aactctttca actgaccttt agacagtgcg cccactttgg ttgccgccac
    16621 ttcaccgttt ttgaacagca gcagagtcgg gataccacgg atgccatatt tcggcgcagt
    16681 gccagggttt tgatcgatgt tcagttttgc aacggtcagt ttgccctgat attcgtcagc
    16741 gatttcatcc agaatcgggg cgatcatttt gcacggaccg caccactctg cccagaaatc
    16801 gacgaggatc gccccgtccg ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg
    16861 aataatttta tcgctcatat ataactccac aggaataagc ctggcgtgtt ggtttcgttg
    16921 ttggtgtaac attaaccaac taaaggttga ctttatttca ccggatacgc tttcgtaaag
    16981 caatagtaag ctgatattct accacactat gagcaaaaca catttaacag aacagaagtt
    17041 ttccgacttc gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa
    17101 ctgtacgccc attcaggcac tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg
    17161 gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct
    17221 tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa ccgcgtgcct taattatggc
    17281 accgacgcgt gaacttgccg tgcagatcca cgccgacgcg gaaccgctgg cggaagctac
    17341 cggcctgaag ctgggcctgg cttacggtgg tgatggctat gacaaacagc tgaaagtgct
    17401 ggaaagcggc gttgacattc tgattggcac cacgggtcgt ttaattgact acgccaagca
    17461 gaaccacatt aacctcggtg ccattcaggt agtggtactg gacgaagccg atcgcatgta
    17521 cgatctaggc tttattaaag atatccgctg gctgttccgc cgtatgccgc ctgcaaatca
    17581 gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga
    17641 gcagatgaac aatgccgaat atattgaagt ggaaccggaa cagaaaacgg gccaccgtat
    17701 taaagaagag cttttctacc cttctaacga agaaaaaatg cgtttgctgc aaacgctgat
    17761 cgaagaagag tggccagaca gagcgattat tttcgccaat accaaacacc gttgtgaaga
    17821 gatctggggc cacctggcgg cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc
    17881 gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt ggcgatctgg atattctggt
    17941 tgccaccgac gttgccgcgc gtggtttgca tattccggca gtgacgcacg tctttaacta
    18001 cgatttaccc gatgactgtg aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg
    18061 cgcaagcggt cactctatca gcctggcgtg tgaagagtat gcattgaatt tgcctgctat
    18121 tgagacctat attggtcact caattccggt aagcaaatac aatccggacg cattgatgac
    18181 cgatctgcca aaaccgctgc gcctcacgcg cccgcgcaca ggcaatggtc cgcgtcgtac
    18241 tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc
    18301 aaactgtatc aagatggcaa gagaatgaat cccgatgagc ttactgtagt aagtgattcg
    18361 ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg ggttccacct
    18421 cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg
    18481 aggtggctgg aagcatccag acgctgacgc gaattaaacg caaagtgcgt ctggctgctg
    18541 gcctgaatag cgaaaatgac ctctctaatg aagcaatgga gcgcggttgg caatgtctgc
    18601 gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg
    18661 cgacattacg acttgccgtc aatgcgggtg attttattgc caaagcgcag gaaatcctcg
    18721 gttgtccggt acaggtgatc agcggtgaag aggaagcgcg tctgatttat cagggcgttg
    18781 ctcacaccac tggtggtgcc gatcagcgcc tggtggtgga tataggcggt gccagtactg
    18841 aactggtaac cggcacgggt gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg
    18901 tcacctggct ggaacgctat tttgccgatc gtaatctggg gcaagaaaat tttgatgctg
    18961 cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct
    19021 ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac
    19081 aggggatgga tgaacgcatt accctggaaa agttgcagca attgaaacag cgagccattc
    19141 attgcggtcg gctggaggaa ctggagattg acgggctgac gctggaacgt gcattagtgt
    19201 tcccgagtgg tctggcgatc ctgatcgcca tttttaccga actgaatatt cagtgtatga
    19261 ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg
    19321 agcaggatat tcgcagccgt acactgcgta atattcaacg ccgctttatg atcgatattg
    19381 atcaggcaca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg gaaaacgaat
    19441 ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg
    19501 gcctgagcgt tgacttcaaa caagctccgc aacacgctgc ttatctggtg cgtaacctgg
    19561 atcttcccgg ttttaccccc gcacagaaga aactgctggc gacgctactg ctcaaccaga
    19621 ctaacccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccaccg cgcgtcgcag
    19681 aacaactctg ccgtttactc cgcctggcta tcatttttgc cagccgtcgc cgtgacgatc
    19741 tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag
    19801 gttggctaac ccaacatccg ctgggtaaag agattattgc tcaggaaaac cagtggcaga
    19861 gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgacg
    19921 catcttctcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc
    19981 cagatggctt tgccctttct gcatccgttc ttcggcactg accactttgc gctcctgttc
    20041 ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg ctcggctccg ggcgcaccag
    20101 ttcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt
    20161 aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga tactgctctg
    20221 gtgcggcaaa aacccttctt ccataccgac catgtagaca taaggaaact ccagcccttt
    20281 tgacgcgtgg agagtcatca gttgcacctg atccagctct tcttcactct caccacgctc
    20341 catcatgtcg cgtaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag
    20401 ttcgctgcct tccagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg
    20461 catttcggca gctttcgtgc tgggcgatgt ttcgtacagc caggattcat aatccatgcc
    20521 gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttctgc
    20581 caaccagtga gtgaagcggg tcaatgcttc ataaccacgt ccgctaagcg tctgactcag
    20641 gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc actcacccag
    20701 ctttttcagc gtcgccgggc caatctctcg cttcggcgtg ttaacgatac gcagaaatgc
    20761 gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg
    20821 acgagagaaa aacgacgtac caccagaaat tttgtacggg atgcggtttt gcatcaggaa
    20881 tttttcaaac acccgcgact gatagttacc gcgataaaga atagcgtaat ctttgtactg
    20941 cgttttattg acgaagtgat gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc
    21001 ttcgttattc gcgcttaaaa cttttagctc tgtgccgtaa cccagttcgg agaacagacg
    21061 cttttcaaag acgtgcgggt tattggcgat caggatgttc gccgctttca aaatacgtcc
    21121 ggaagagcgg tagttctgct caagcttaat caccttcagc gccggaaaat cctggctcag
    21181 cagcaccagg ttttgcggac gtgcgccgcg ccaggagtag atcgactggt catcgtcacc
    21241 caccacggta aagcgcgcgc ggctgcccac cagcagtttc accagttcat actggctggt
    21301 gttggtgtcc tgatattcgt ccaccagcag gtagcgaatt ttgttctgcc agcgctcgcg
    21361 gacttcttca ttgcgttgca gaagtagcgt cggcaataaa atcagatcat cgaagtcgag
    21421 cacattacag gctttcaggt gtgcatcata cagcccataa caatgggcaa aaatacggtc
    21481 ccgctcgcca atcgcactgg ctgccgcctg ggacggtgtt ttgagatcgt tcttccagtt
    21541 agagatggtc gaaatcaact gttgcaggag aactttgtca tcttcaatca gcccttcggt
    21601 caactctttg agcaacgcca gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat
    21661 ccccagcgcc gcatactcac gcttgatgat atccagcccc agcgtgtgga aggttgagat
    21721 catcagccca tgcgcctctt tgcgacccag cgtctgtccg acacgctctt tcatctcgcg
    21781 cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac cgcacccgcg
    21841 gatcagatgg gcgattttat tggtgataac acgagtttta ccggaacccg cacccgccag
    21901 caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt ttagacgcat
    21961 aggaaaatcg ctcaatgaaa gtcgggaggg gaaaataaca gcgtggtagt atagccagcc
    22021 tcatccttac cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
    22081 taaaagaaga gaaactggct ctggatcttc tcgtgcagat taagaacggt gccgatttcg
    22141 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
    22201 aattccgcca gggtcagatg gttccggcgt tcgataaagt tgtgttctct tgtccggtac
    22261 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
    22321 gtaactaata gcaaggcctt ctccgggaga aggccttgaa ctctgaatcg ctattaaccc
    22381 gcaacagcaa tacgtttcat atccgtcata tagccgcgca gtttcttacc tacttgctca
    22441 atcgcatggc tgcgaatcgc ttcatttaca tcacgcagct gcgcgttatc taccgcacct
    22501 tccggaatag ctttacccaa atcgcccggt tgcagttctg ccataaacgg tttcagcagc
    22561 ggcacacaag cgtaagagaa cagatagtta ccgtactcgg cagtatcaga gataaccacg
    22621 ttcatttcat acagacgctt acggacgatg gtgttggcaa tcagcggcag ttcgtgcagt
    22681 gattcatagt aagcagattc ttcgatgatg ccggaatcaa ccatggtttc gaacgccagt
    22741 tcaacgcccg ctttcaccat cgcgatcatc agtacgcctt tatcgaagta ctcctgctcg
    22801 ccgattttgc cttcatactg cggcgcggtt tcgaaggcgg ttttgccggt ctcttcacgc
    22861 caggtcagca gtttcttatc atcgttggcc cagtccgcca tcataccgga ggagaattcg
    22921 ccggagatga tgtcgtccat atgtttctgg aacagcggtg ccatgatctc tttcagctgt
    22981 tcagacaacg cgtaagcacg cagtttcgcc gggttagaga gacggtccat catcagtgtg
    23041 ataccgccct gcttcagcgc ttcggtgatg gtttcccaac cgaactgaat tagtttttct
    23101 gcgtatgccg gatcggtgcc ctcttccacc agcttgtcga agcacagcag cgagccagcc
    23161 tgcaacatac cgcacaggat ggtttgctca cccatcaggt cagatttcac ttccgcaacg
    23221 aaggacgatt ccagcacgcc cgcacggtga ccaccggttg ctgccgccca tgctttagcg
    23281 atcgccatgc cttcgccttt cggatcgttt tccgggtgaa cggcaatcag cgtcggtacg
    23341 ccgaatccac gtttgtactc ttcacgcact tcggtgccag ggcatttcgg cgcaaccatg
    23401 actacggtga tgtctttacg gatctgctcg cccacttcga cgatgttgaa accgtgcgag
    23461 taacccagcg ccgcgccgtc tttcatcagt ggctgtacgg tgcgcactac atcagagtgc
    23521 tgcttgtccg gcgtcaggtt aaccaccaga tccgcctgtg ggatcagttc ttcgtaagta
    23581 cccactttaa aaccattttc ggtcgcttta cgccaggacg cgcgcttctc ggcaatcgct
    23641 tctttacgca gagcgtagga gatatcgaga ccagaatcac gcatgttcag accctggttc
    23701 agaccttgtg cgccacagcc gacgatgact acttttttac cctgaaggta gctcgcgcca
    23761 tcggcgaatt catcgcgccc cataaagcga catttgccca gctgtgccag ctgctgacgc
    23821 agattcagtg tattgaagta gttagccatg gtgattcctc gtgatgttgt gcttcttatt
    23881 gttcggttcg cgtttgcgag attgaattca ctatatgaca ggaaatttat tgcggaaatt
    23941 gatatattca caacgtcaca ttgcaatttc tgcaacgtca acattgaggg ctgcgccatt
    24001 ggatttacgc gatctgaaaa ccttcctgca tctggcggaa agccgccatt ttggccgcag
    24061 cgcgcgggcg atgcacgtta gcccgtccac gctctcacgg cagattcagc gcctggaaga
    24121 agatctcggc cagccgctgt ttgtgcgcga taaccgcacg gtgacgctga ctgaagcggg
    24181 cgaagaactg cgcgttttcg cccagcaaac gctgttgcag tatcagcagc tgcgccacac
    24241 catcgaccag caagggccgt tgctctatgg cgaattacat atcttctgct cggtgaccgc
    24301 cgcttacagc catctgccgc cgattctgga tcgcttccgc gcggaacacc cgtcggtgga
    24361 gatcaaactc actaccggtg atgcggcaga tgcaatggaa aaagtcgtca caggtgaagc
    24421 tgatctggcg attgccggga agccagagac tctgcccggc gcggtggcgt tttcgatgtt
    24481 ggagaatctg gcagtggtgt tgattgcacc cgcattgccc tgcccggtgc gtaatcaggt
    24541 ttctgtcgaa aagccggact ggtcaacggt accgtttatc atggccgatc aggggccggt
    24601 gcgccgccgc attgagttgt ggttccgacg caataaaatc agtaacccga tgatttacgc
    24661 cacggttggc gggcatgaag cgatggtatc gatggtggcg ctcggctgtg gcgtggcatt
    24721 gttgccggaa gtggtgctgg aaaacagccc cgaaccggtg cgtaaccgcg tgatgatttt
    24781 agagcgcagc gatgagaaaa cgccgtttga gctgggcgtg tgtgcgcaga aaaagcggct
    24841 acatgagccg ctaattgagg cattctggaa gattttgccg aaccattaac ccgccaaaaa
    24901 gaacctgaac gccgggttat tggtttcgtc gtggcaatcg tagcccagct cattcagccg
    24961 ggtttcgaaa tccggttcat ggtcgccaag ttcgaacgcc gccagtacgc gcccgtagtc
    25021 ggtgccatgg ctgcgatagt ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag
    25081 gaagcgcagc agcgcgcccg gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa
    25141 cggatgtgac ggacgcccgc cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc
    25201 ggagagatcc accacgctgt agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc
    25261 ttcgaggccg cggctcaggc gcacgccgac aaagatgcag gcgtttttgg catcggcaaa
    25321 acggtagttg aactcggtga ccgatcgccc gccaagcagt tggcagaatt tgaggaagct
    25381 gcctttttct tccggaatgg tcaccgccaa caacgcttca cgctgttcgc ccagttcgca
    25441 gcgttccgag acgtagcgca ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc
    25501 cagccgttcg ccgcgaatgt tgtgctgggc gatatatttt ttcattcccg ccagcgccag
    25561 cgcgccagag ggttccgcca ccgcacgcac atcttcgaat aaatccttca tcgccgcaca
    25621 gatcgcatcg ctatcgacgg tgatgatgtc gtcgagatac tcctggcata aacggaaggt
    25681 ttcgtcaccg atgcgtttta ccgcgacacc ttcggcgaac agtcccacgc gtggcagatc
    25741 aaccggatga cccgcatcca gcgctgcttt caggcaggcg gagtcttccg cttctacggc
    25801 gatcactttg atttgcggca tcagttgttt gatcagcacc gctacgcccg ccgccagacc
    25861 gccgccgccg actggcacaa atacgcggtc gagatgggcg tcctgctgga gcagttccag
    25921 cgccagcgtg ccttgcccgg caatcaccat cggatggtcg aacggcggca cccaggtgaa
    25981 cccctgctgc tgtgacagtt cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg
    26041 aagcagcact tcgccgccga atccacgtac cgcatcgact ttgatgtcgg cggtggcggt
    26101 tggcataacg atcagggcct tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc
    26161 gtggttaccc gcagaagcag tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc
    26221 catcatggcg tatgcgccgc gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt
    26281 caccagaatg acgttatcaa gacgcgacga cagtttttcc attttttgta gcggcgtaac
    26341 ctgcgccgcc tcgtaaaccg gcgcgcgcag cactgctctt aaatattcgg caccttccgg
    26401 agcaccggac aggggttgcg agtcagccat tattaacccc ccagtttcga tttatcgcgc
    26461 accgcacctt tgtcggcgct ggttgccagg ctggcgtagg cgcacagggc aaaggaaacc
    26521 tgacgttcac ggtttttcgg cgtccaggct ttgtcacccc gagcttcctg cgcttcacga
    26581 cgcgccgcca gttcggcatc gcttacctgt aactgaatgc cacggttcgg gatgtcgata
    26641 gcgatcaggt caccatcttc aatcagacca atgctgccgc cgcttgccgc ttccggtgag
    26701 acgtggccga tggaaagacc agaggtgccg ccagagaaac gaccgtcggt gatcagcgca
    26761 caggctttac cgagacccat tgatttcagg aagctggttg ggtagagcat ttcctgcatg
    26821 ccagggccgc ctttcgggcc ttcatagcgg atcaccacca catcgcccgc gacaactttg
    26881 ccaccgagga tcgcttctac cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg
    26941 aatttgagga tgctgtcatc gacgcccgcg gttttcacga tgcagccgtt ttcagcaaaa
    27001 ttaccgtaga gcaccgccag gccgccgtct ttgctgtagg cgtgttccag cgagcggata
    27061 cagccgtttg cgcgatcgtc atcgagagaa tcccaacggc aatcctgcga gaatgcctgt
    27121 gtagtacgaa tgcccgcagg gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc
    27181 agcataacgt cgtattgttc cagagtttgc ggcaacgtca ggccaagtac gtttttcaca
    27241 tcacggttca gtaaccccgc gcgatccagt tcaccgagaa taccgataac accaccagca
    27301 cggtgaacat cttccatatg gtatttttgg gtgctcggcg caactttgca taactgcggg
    27361 actttacgcg aaagtttgtc gatatcactc atggtgaagt cgatttccgc ttcctgcgcc
    27421 gccgccagca ggtgaagtac cgtgttggta gagccgccca tcgcgatatc cagcgtcatg
    27481 gcattttcaa acgccgcttt gctagcaata ttgcgcggca gtgcactttc gtcgttttgc
    27541 tcgtagtaac gtttggtcaa ttcaacaatg cgtttaccgg cattgaggaa caactgctta
    27601 cggtccgcgt gggttgccag cagcgagccg ttgcccggct gcgacaggcc cagcgcttcg
    27661 gtcaggcagt tcattgagtt agcggtaaac atcccggagc aggagccgca ggtcggacac
    27721 gcggaacgtt caacctgatc gctctgggag tcagaaactt tcgggtctgc gccctggatc
    27781 atcgcatcaa ccagatcgag tttgattatc tgatcggaca gtttggtttt cccggcctcc
    27841 atcgggccgc cggaaacaaa gatcaccgga atattcaggc gcagggaagc catcagcatc
    27901 cccggggtga ttttgtcgca gttagagatg cagaccatgg cgtcggcgca gtgggcgttg
    27961 accatatact caacggaatc agcgatcagt tcgcgagatg gaagtgaata aagcatcccc
    28021 ccgtggccca tggcaatccc atcatccacc gcaatggtgt tgaactcttt ggcaacgccg
    28081 ccagccgctt caatttgttc ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc
    28141 ggtacaaatt gggtgaacga gttcacaacc gcgataatcg gcttgccaaa atcggcgtcg
    28201 gtcattccgg tggcgcgcca cagcgcacga gcacccgcca tattacgacc atgagtggtg
    28261 gtggcggaac ggtacttagg catactttat ttactcccag tgtctgtctc gtaaatggga
    28321 cggtgcgtgc cgtcccattt tttgtattta ttgattaact tgatctaacc agccccattt
    28381 atcttcggtt tcgccagtga agaggccgaa gaaggcttgc tgaatgcgtt tggtaaccgg
    28441 gccacaacgg ccttcgccaa tctgaatacc gtctacgctg cgcactggcg tgatttctgc
    28501 cgccgtaccg gacataaaga cttcatccgc caagtacagg gattcgcgcg acagcacctg
    28561 ctcacgcact tcaattccca gctctttcgc cagtttgatg atggcatcac gggtaatacc
    28621 cggcagcgcg gaggaggtga acggtggggt gaacagcaca ccatctttca cttcaaacag
    28681 gttttcgcct gcgccttcag agatataacc gttcacatcc agcgcgatac cttcctgata
    28741 accgtggcgg cgcgcttcgc tacccaccag cagggaagag agatagttac caccggcttt
    28801 tgccgcagtt gggatggtgt ttggtgctgc gcggttccag gaggaaacca tcgcatcgat
    28861 cccctgctcc agcgcttctg cgcccagata cgctccccac gggaaagcgg cgataatcac
    28921 atcggttgag tatcccgctg gcgggtttac tcccatgcca acatcaccga cgaagatcag
    28981 cggacggata taggcgctgg tgagattgtt tttgcggatc acgtcacgac aagcttccat
    29041 cagctcatca atgctctgtg aaaccgggaa gcgatagatt ttggcggagt catgcagacg
    29101 ctgcatatgc tcacgatggc ggaatacaac cggtcctttg tgtgagtcgt agcaacggat
    29161 gccttcaaaa accgaggtgc catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc
    29221 ttcccagcga accatctccc cattgaacca aatgtaatca gctttcttcg tggtcatttt
    29281 tatattcctt ttgcgctcag gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg
    29341 caacgtgtgc gacgtccacc agtttattta actgactaaa cagtaagtcg accgaccgtg
    29401 ggctggcaac ggtcaattcg atatttatat tttgtgcatc gctggcggca gccatattca
    29461 ttgagcagac gtggaaacca cgatgacgca ccacgcgtaa aacacgttct aaggtttccg
    29521 gattgaagcg agccgataca ttgacctgat gttgcatcat gataatttct ccaacatttc
    29581 tgaattactg gcacctggcg gcaccagcgg ccagacgttc tcaagttcgt cgattgagac
    29641 atgaagcagg tatggcccat cactgttcag catggtgtcg agtgccgctt caacctggtc
    29701 tttacgggtg atgtgttggc cagggatgcc gaaggcgctg gctaacatga ggaaatcggg
    29761 gttatcggta agggtggttt cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg
    29821 aaccatccct aaccgttggt tatcgagtaa gacgattttc aacggtaact gcttgcgttt
    29881 tacggtgccc agctcttgca cattcatcat gaaagagccg tcaccggaga tacagacaac
    29941 ggtatcgttc ggtcgcgcga cttgtgcgcc aactgccgcc ggtaaaccaa aacccatggt
    30001 acctaagccg ctggaggtga tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc
    30061 ccacatctgg tgctgcccca catctgtggt cacgacgcaa tccgcaggtt tacgatccga
    30121 cagttgtttt aacaacaacg gagcgtagat agcgtcaccg ggatggtcgt aacgccagga
    30181 atgttcatca cgcagctgcg cgcagtgttg ctgccagtca ttgatattta acggctgctg
    30241 taatgctggt aacagagcat ttaaatcacc ttgtaatgcc acatgtgcct gacgcagctt
    30301 gttcatttct gccgggtcga tatccatatg gataacactg gcgtgtggtg cgaaggtgtt
    30361 cagtttgccg gtcacccggt catcaaaacg tgcacccacg gcgatcagca ggtcacactc
    30421 ctgcaccgcg aagtttgccg ctttggttcc gtgcatcccc agcatgccca gatagtacgg
    30481 ataatctgct tctactgcgc ccagcccttt cagcgtacag gtggcaggca tttttgtggc
    30541 agcgagaaat tcacgcaaag ccggaactgc ctgcgccatc cccacgccac cgccaacgta
    30601 cagcatcggt ttttgcgctt ttgccagcat ctggcgcgct tgctcgactt cggcatgtgg
    30661 gaaagtcact tcgttttcaa cggtggtgaa ccacggttcc agatcgccgc tggctaactg
    30721 gatatctttt gggatatcga ccagaaccgg accaggacga cctgagctgg caacgtcgaa
    30781 tgcttcagcc atgatgcgcg gcaactcttc cagagactgc accagaaagc tgtgcttggt
    30841 acaggctaac gacaatccca ggacatccac ttcctgaaat gcgtcggtgc cgataaacgg
    30901 tgcggacacc tgaccagtga tggcaacgac agggatggaa tctaacagtg cgtccgcaag
    30961 cccggttatc aggttggttg cgcccggacc agacgtggcg atacatacgc cagttttacc
    31021 ggtagcacgg gcataaccga tagccgccat tgccgcaccc tgctcatgtc ggcacagcaa
    31081 gtgctccacg ccgccgtcat acaatgcatc gtaaaccggc ataattgcgc cacccggata
    31141 accgaaaacg gtgttcacac cctgtgcccg caacgcatgt accacccact gtgcgccatt
    31201 catagttagt tccccgtcct gaatcttgag aaacagaatt ttgtgctgtt attcattgtc
    31261 tgctcctcgg ttatgttttt aaggtcaaaa aaacccccgg acctttcggt gcgggggtct
    31321 tagttcgtta aggcttgatc tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg
    31381 gataataatc accaccacgc taatcacgac caggctaatc actcgtagaa gggctgtcat
    31441 tttgtctttt cttgcatctt gttcgaagga atgcctaaag agttaccata gattttgcga
    31501 atagcacaag atatttttgg gcctattttt atgatggctt taataatttc aatagaaatt
    31561 gttgtttttt aaaaagaaaa tagtgagtga aaaaattcaa tttcagtata aatgttgttc
    31621 ccttcctcgt agttcatcct ttgtccgctg catttttgcg agcatcattc caccgccaga
    31681 acaactcaac ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc
    31741 gggctacagc agcgatatct tatgcacata atggtgactc aaggagggtt tatgtcactg
    31801 tcaattgttc atacccgcgc agccctggaa gtaaatgcgc ccccaatcac tgttgaggta
    31861 catatcagta aaggtctacc cggcttaacg atggtgggct taacagaaac aacggtgaaa
    31921 gaagctcgcg atcgcgtgcg cagcgccatt atcaatagcg gatatgaata tccggcgaaa
    31981 aaaatcacca tcaaccttgc tccagccgat ctgccaaaag aagggggacg atatgattta
    32041 cctatcgcca ttgcgttgct ggcggcctca gaacagctta cagccaataa gttagatgaa
    32101 tatgaattag tcggagaact agcgcttaca ggcgctctgc gtggcgttcc cggtgcaatc
    32161 tccagtgcaa ctgaagctat taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa
    32221 gatgaagtgg ggctaattaa cggtgaagga tgcctgatag ccgatcatct gcaggctgtc
    32281 tgtgcgtttc tggaaggtaa gaacactctc gaacgcccga aaccaactga tgcagtatcc
    32341 cgggcgctac aacatgatct cagtgatgtt gtcggtcagg agcaaggaaa acgaggactg
    32401 gaaattaccg ccgctggtgg gcacaacctt ttactgattg ggccgccggg aacaggtaaa
    32461 acaatgctcg ccagccgtat taatggcctg ttgccagatt taagcaatga agaggcactg
    32521 gagagcgctg cgatattaag tctggtaaat gctgaatcag tacaaaaaca atggcggcag
    32581 cgcccgttcc gctcacctca tcacagtgcg tcgttaactg cgatggtggg cggtggtgca
    32641 attccagggc caggtgaaat ttcgttggcg cataacggcg tgctttttct tgatgagcta
    32701 cctgaatttg aacggcgtac actggatgcc ttgcgagagc cgattgaatc cgggcagatc
    32761 catctttcac gcacacgagc aaaaataacc tatccagccc gtttccagct tgttgcggcg
    32821 atgaatccca gccctaccgg acattatcag ggaaaccata accgctgcac gccagaacag
    32881 acattacgtt atctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    32941 agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca gagagcgcca
    33001 atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac gccaattctg
    33061 caaacttgag agcgaagatg cgatgtggtt ggaagaaacg ctgatccatc tggggttatc
    33121 gattcgtgcc tggcagcggt tactgaaagt tgcacgaacc attgctgata ttgatcagtc
    33181 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    33241 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    33301 cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    33361 gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    33421 tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg
    33481 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    33541 cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt ttattgtcga
    33601 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    33661 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    33721 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    33781 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    33841 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    33901 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    33961 cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataacg
    34021 agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag tggttgggac
    34081 gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    34141 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    34201 cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta gcgctttata
    34261 ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag tgggggccag
    34321 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca
    34381 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    34441 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    34501 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt
    34561 tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag
    34621 aaggatttac ttcggagagg attatttcag ataaaaaaaa tccttagctt tcgctaagga
    34681 tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg agaccggtgc
    34741 tctaccaatt gaactacgcc cctaattagg gtggcggaac ggacgggact cgaacccgcg
    34801 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
    34861 tacaaccacc ggttttacga ccggctt
//
LOCUS       DAJTOW010000034        34350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTOW010000034 DAJTOW010000000
VERSION     DAJTOW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.034"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..332)
                     /locus_tag="OO244_001757"
     CDS             complement(<1..332)
                     /locus_tag="OO244_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663611.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO244_001758"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO244_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5663612.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO244_001759"
     CDS             complement(1693..2073)
                     /locus_tag="OO244_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5663613.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO244_001760"
     CDS             complement(2087..2503)
                     /locus_tag="OO244_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663614.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO244_001761"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO244_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5663615.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO244_001762"
     CDS             complement(3504..3971)
                     /locus_tag="OO244_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5663616.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO244_001763"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO244_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5663617.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO244_001764"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO244_001764"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5663618.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO244_001765"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO244_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5663619.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO244_001766"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO244_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5663620.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO244_001767"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO244_001767"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5663621.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO244_001768"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO244_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5663622.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO244_001769"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO244_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5663623.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO244_001770"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO244_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5663624.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO244_001771"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO244_001771"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5663625.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO244_001772"
     CDS             complement(14626..15441)
                     /locus_tag="OO244_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5663626.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO244_001773"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO244_001773"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5663627.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO244_001774"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO244_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5663628.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMALEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO244_001775"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO244_001775"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5663629.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDVYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO244_001776"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO244_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5663630.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKHQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO244_001777"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO244_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5663631.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO244_001778"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO244_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5663632.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO244_001779"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO244_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5663633.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO244_001780"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO244_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5663634.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO244_001781"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO244_001781"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5663635.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO244_001782"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO244_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5663636.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO244_001783"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO244_001783"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5663637.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO244_001784"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO244_001784"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5663638.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO244_001785"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO244_001785"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5663639.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO244_001786"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO244_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5663640.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO244_001787"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO244_001787"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5663641.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO244_001788"
     CDS             31575..32189
                     /locus_tag="OO244_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5663642.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO244_001789"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO244_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5663643.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLTSGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO244_001790"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO244_001790"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5663644.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8080 a         8677 c         9276 g         8317 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac agtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 tggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgt atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagca ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgac ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagcagtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag
//
LOCUS       DAJTOW010000035        33525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTOW010000035 DAJTOW010000000
VERSION     DAJTOW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.035"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            65..1342
                     /gene="waaA"
                     /locus_tag="OO244_001791"
     CDS             65..1342
                     /gene="waaA"
                     /locus_tag="OO244_001791"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5663645.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1350..1829
                     /gene="coaD"
                     /locus_tag="OO244_001792"
     CDS             1350..1829
                     /gene="coaD"
                     /locus_tag="OO244_001792"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5663646.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1868..2677)
                     /gene="mutM"
                     /locus_tag="OO244_001793"
     CDS             complement(1868..2677)
                     /gene="mutM"
                     /locus_tag="OO244_001793"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5663647.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2775..2942)
                     /gene="rpmG"
                     /locus_tag="OO244_001794"
     CDS             complement(2775..2942)
                     /gene="rpmG"
                     /locus_tag="OO244_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5663648.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2963..3199)
                     /gene="rpmB"
                     /locus_tag="OO244_001795"
     CDS             complement(2963..3199)
                     /gene="rpmB"
                     /locus_tag="OO244_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5663649.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3416..4084)
                     /gene="radC"
                     /locus_tag="OO244_001796"
     CDS             complement(3416..4084)
                     /gene="radC"
                     /locus_tag="OO244_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5663650.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4256..5476
                     /gene="coaBC"
                     /locus_tag="OO244_001797"
     CDS             4256..5476
                     /gene="coaBC"
                     /locus_tag="OO244_001797"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5663651.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5454..5912
                     /gene="dut"
                     /locus_tag="OO244_001798"
     CDS             5454..5912
                     /gene="dut"
                     /locus_tag="OO244_001798"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5663652.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6019..6615
                     /gene="slmA"
                     /locus_tag="OO244_001799"
     CDS             6019..6615
                     /gene="slmA"
                     /locus_tag="OO244_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5663653.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6652..7293)
                     /gene="pyrE"
                     /locus_tag="OO244_001800"
     CDS             complement(6652..7293)
                     /gene="pyrE"
                     /locus_tag="OO244_001800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5663654.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7359..8075)
                     /gene="rph"
                     /locus_tag="OO244_001801"
     CDS             complement(7359..8075)
                     /gene="rph"
                     /locus_tag="OO244_001801"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5663655.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8202..9065
                     /locus_tag="OO244_001802"
     CDS             8202..9065
                     /locus_tag="OO244_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5663656.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9286..10110
                     /gene="dinD"
                     /locus_tag="OO244_001803"
     CDS             9286..10110
                     /gene="dinD"
                     /locus_tag="OO244_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5663657.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10401..11018
                     /locus_tag="OO244_001804"
     CDS             10401..11018
                     /locus_tag="OO244_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5663658.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11015..12697)
                     /gene="ligB"
                     /locus_tag="OO244_001805"
     CDS             complement(11015..12697)
                     /gene="ligB"
                     /locus_tag="OO244_001805"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5663659.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12955..13578
                     /gene="gmk"
                     /locus_tag="OO244_001806"
     CDS             12955..13578
                     /gene="gmk"
                     /locus_tag="OO244_001806"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5663660.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13633..13908
                     /gene="rpoZ"
                     /locus_tag="OO244_001807"
     CDS             13633..13908
                     /gene="rpoZ"
                     /locus_tag="OO244_001807"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5663661.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13927..16035
                     /gene="spoT"
                     /locus_tag="OO244_001808"
     CDS             13927..16035
                     /gene="spoT"
                     /locus_tag="OO244_001808"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5663662.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16042..16731
                     /gene="trmH"
                     /locus_tag="OO244_001809"
     CDS             16042..16731
                     /gene="trmH"
                     /locus_tag="OO244_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5663663.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16737..18818
                     /gene="recG"
                     /locus_tag="OO244_001810"
     CDS             16737..18818
                     /gene="recG"
                     /locus_tag="OO244_001810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5663664.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18852..20057)
                     /gene="gltS"
                     /locus_tag="OO244_001811"
     CDS             complement(18852..20057)
                     /gene="gltS"
                     /locus_tag="OO244_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5663665.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20337..21728
                     /gene="xanP"
                     /locus_tag="OO244_001812"
     CDS             20337..21728
                     /gene="xanP"
                     /locus_tag="OO244_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5663666.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21849..23558
                     /locus_tag="OO244_001813"
     CDS             21849..23558
                     /locus_tag="OO244_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5663667.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23611..25929)
                     /gene="yicI"
                     /locus_tag="OO244_001814"
     CDS             complement(23611..25929)
                     /gene="yicI"
                     /locus_tag="OO244_001814"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5663668.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25939..27322)
                     /locus_tag="OO244_001815"
                     /pseudo
     CDS             complement(25939..27322)
                     /locus_tag="OO244_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27615..27709
                     /locus_tag="OO244_001816"
     tRNA            27615..27709
                     /locus_tag="OO244_001816"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27649..27651,aa:Sec,seq:tca)
     gene            27981..29192
                     /locus_tag="OO244_001817"
     CDS             27981..29192
                     /locus_tag="OO244_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5663669.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29604..30647
                     /locus_tag="OO244_001818"
     CDS             29604..30647
                     /locus_tag="OO244_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5663670.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31162..31590
                     /locus_tag="OO244_001819"
                     /pseudo
     CDS             31162..31590
                     /locus_tag="OO244_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31620..32771)
                     /locus_tag="OO244_001820"
                     /pseudo
     CDS             complement(31620..32771)
                     /locus_tag="OO244_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32836..33484
                     /locus_tag="OO244_001821"
                     /pseudo
     CDS             32836..33484
                     /locus_tag="OO244_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8395 a         8814 c         8260 g         8056 t
ORIGIN      
        1 gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa gtcagctatt
       61 tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc tgatctggat
      121 acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg gtgaacgtta
      181 cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg tctccgtcgg
      241 tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc ctgatttacc
      301 gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg ctttcgggaa
      361 ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc gtttcctgaa
      421 taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta acctgattgc
      481 ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct ctgcccgctc
      541 ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc gtattacgct
      601 gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg caaaaaataa
      661 ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc agttggctgc
      721 taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga ttgccaccag
      781 cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt tacagcaatt
      841 cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg atgcgattaa
      901 ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag tcccctccac
      961 cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt acggcattgc
     1021 cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc cgctggaagc
     1081 tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta aagacatttg
     1141 cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta cgcttgcaaa
     1201 agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc gtcatgccgt
     1261 tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc tggaacctta
     1321 cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat ccgggtactt
     1381 tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag atgttcgatc
     1441 acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc ctggaagagc
     1501 gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg gtcgggttta
     1561 gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt cgtggcctgc
     1621 gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc cacttaatgc
     1681 cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc tcttcatcgt
     1741 tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg gagaatgtcc
     1801 atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc catccggctc
     1861 gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca tgtttagtcg
     1921 ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa acctgcaatt
     1981 cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc gttgtaccac
     2041 cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat tcacactctg
     2101 ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac agtgattcgc
     2161 tggcatagat attccctacc cctaccacca gcttgttatc catcagccac ggtttaatcg
     2221 ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg tcgctaagcg
     2281 gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc cacagccagg
     2341 caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc accaaatcca
     2401 catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg ccagacatcc
     2461 ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta gcccgccgct
     2521 gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc ggccagcgca
     2581 agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc ggttctatgc
     2641 cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga atgaacagat
     2701 gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg tttttcgtta
     2761 catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg acgaacaact
     2821 ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt cgtagtggta
     2881 tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat acctttagcc
     2941 atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt tcagccagaa
     3001 ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg cgcagggtga
     3061 caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc aggaaacggc
     3121 gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc ttgccagtaa
     3181 cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag cttcgtatgg
     3241 ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt gaactctcga
     3301 ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata cgctgactca
     3361 gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata gcgggttaaa
     3421 tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata tggtcgagca
     3481 cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt ttatccgctt
     3541 tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc gaggcgttta
     3601 tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt agcgtgccgg
     3661 aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc accataaaga
     3721 tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc tccgggctga
     3781 gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt tcagcaatcc
     3841 cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat tgttcatatt
     3901 cagaggttaa caagccataa agagagccga aattctccag catctctttt gccagggtta
     3961 atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg acatccgtta
     4021 aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt gcattgtttt
     4081 tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt aaatcgacgg
     4141 ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg cacgacaaca
     4201 aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa tcatcatgag
     4261 cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct ataaaacccc
     4321 tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca tgaccgaagc
     4381 ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc ccgtttccga
     4441 cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg gtaaatgggc
     4501 tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg ctgccggaat
     4561 ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag ccgtgctccc
     4621 cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag aggtgcttgc
     4681 ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg gtgatatcgg
     4741 tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc atttttcgcc
     4801 cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc gtgaaccgct
     4861 cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg ctatcgccgc
     4921 cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt cactaccgac
     4981 gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag ccgccgtgaa
     5041 tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg attatcgcgc
     5101 agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg aattaacaat
     5161 aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag accatcgacc
     5221 ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc ggcaaaaacg
     5281 tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa ctcaaggatt
     5341 taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag tcttaccgct
     5401 tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc gttatgatga
     5461 aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg ctcccgactt
     5521 atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac gccgtagaac
     5581 tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt gccgatcctt
     5641 cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt atcgtgcttg
     5701 gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt tccgtgtgga
     5761 accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag atgatttttg
     5821 ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc gaccgcggtg
     5881 aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata acgtcataac
     5941 atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa gtgcttattt
     6001 tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg tcgcgaggaa
     6061 atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg tatcacgacg
     6121 gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca cttccccagt
     6181 aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat tactcgcatc
     6241 aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat tgtgttgctg
     6301 cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg tcatgcgcta
     6361 atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg tattgaagcg
     6421 cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta caccaccgat
     6481 gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc acgttttgtc
     6541 cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc gctaattgcc
     6601 gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc tttaaacgcc
     6661 aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg gcttctcttc
     6721 cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt tgtaatcacg
     6781 ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat cgagcgaaat
     6841 caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact cgcgaatcgc
     6901 cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg cgctaccaac
     6961 cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt acggcaggtc
     7021 caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga tccctttgta
     7081 agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt cagcgtaaaa
     7141 acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga agaaataggg
     7201 gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct tgctaagcgc
     7261 aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct tacttttcta
     7321 cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag cgccgccttc
     7381 tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt gagtagctct
     7441 tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg cccgtcttcg
     7501 gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc cagatcgcaa
     7561 attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt catcgggttg
     7621 gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc cacgcaggca
     7681 cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca gtccagcgta
     7741 atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg ggcgatcaga
     7801 cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg agcgttacgg
     7861 gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg gccctgacct
     7921 ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt ggtatcgcca
     7981 aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag ggtaacggga
     8041 cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc ttgaaacaaa
     8101 tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag gcatcgatgg
     8161 ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc agtatgaccg
     8221 cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa atgcgctcgg
     8281 taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt agccttgaac
     8341 ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa tgtaccctgc
     8401 gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa ctggctaaac
     8461 agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa atcaacccgg
     8521 ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc gacgcgattg
     8581 ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc gcgcgcgaaa
     8641 ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc accgccgaag
     8701 tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag cgtctggtcg
     8761 cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag gagttggttc
     8821 tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg catgttaaag
     8881 agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac tttatgatgc
     8941 aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc gaagtgacaa
     9001 actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt cagaatattg
     9061 agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc actcaaacag
     9121 agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc cagattttgc
     9181 gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat ataaatacag
     9241 ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa cgaacatcat
     9301 caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg gtcagcaaga
     9361 caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt attaacgcgc
     9421 gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga ggagatcctc
     9481 gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt actctcccgc
     9541 tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat tgcggcaggg
     9601 cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga agcatttaag
     9661 caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga acataacaaa
     9721 caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt cgccatattt
     9781 cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat ccaccaactg
     9841 aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga actggcggct
     9901 aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt gaattcaaaa
     9961 cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac cattcaggaa
    10021 cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa gcagttggaa
    10081 aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt tgttgattga
    10141 gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa acattgcacc
    10201 tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca gacaagaagt
    10261 cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa gttatcaatt
    10321 tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac cctgccagac
    10381 agatttttag ggagagaacc atgctgttac acattttgta tttggttggc attactgccg
    10441 aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt ggcgtaatta
    10501 ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact
    10561 atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac
    10621 tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg
    10681 cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg
    10741 gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc gttttgcgcg
    10801 atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc ggtgtctcat
    10861 ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga
    10921 ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt cttaagctgg
    10981 gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct
    11041 gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct
    11101 gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc gtgctaaata
    11161 aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt agcggtattc
    11221 ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac tgatgccata
    11281 actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata
    11341 aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc caaccggcct
    11401 caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag cgtgaaatga
    11461 actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag cggttttctg
    11521 gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac
    11581 cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc
    11641 gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga
    11701 gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt
    11761 cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc tggcccggta
    11821 gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg gtgacgaagg
    11881 gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca tttttaaccg
    11941 cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg
    12001 tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt
    12061 cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca
    12121 tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg
    12181 ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc tgcgtccagt
    12241 cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa
    12301 ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca
    12361 tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg gcaaccggat
    12421 gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc acaaagcagc
    12481 gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt
    12541 ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt
    12601 cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc
    12661 acaagatact tattaatatc gccatccata ctttcatctt cacctccgct gtagtcaggc
    12721 tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt ctgcgaggac
    12781 gcttcctgag tatcgtcttt attgcagtaa atcacaggca aatgcggaaa cagctacgca
    12841 aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatga ccgcgccgtg
    12901 tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct
    12961 caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag cctgattcag
    13021 gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc
    13081 caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt
    13141 aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat
    13201 ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat
    13261 atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt
    13321 attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc
    13381 gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa
    13441 tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt
    13501 attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc
    13561 aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg
    13621 agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt
    13681 tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat
    13741 ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa
    13801 ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagctggaa
    13861 gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg
    13921 tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac
    13981 caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca
    14041 cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat tctggccgag
    14101 atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagatact
    14161 cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag
    14221 ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac
    14281 tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc
    14341 gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc
    14401 gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt
    14461 aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc
    14521 aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa aatcctctcg
    14581 gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg tcgcgagaag
    14641 catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg
    14701 gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg
    14761 caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca
    14821 aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt
    14881 gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt tgccgcgcac
    14941 tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg
    15001 atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc
    15061 gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc
    15121 gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt
    15181 catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc
    15241 agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg
    15301 aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag
    15361 cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt
    15421 aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca
    15481 acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg
    15541 aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg
    15601 cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg ccctattcct
    15661 ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc
    15721 tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat
    15781 aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc
    15841 gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat
    15901 acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc tcgtgaccgt
    15961 gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc
    16021 cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc
    16081 gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt
    16141 tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct
    16201 ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg
    16261 aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg catgcagatt
    16321 ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta cactcgcccg
    16381 acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg
    16441 gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc
    16501 tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt
    16561 gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg
    16621 gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc
    16681 gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga
    16741 aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta
    16801 gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc
    16861 tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg
    16921 ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga
    16981 cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg
    17041 caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg
    17101 gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc
    17161 cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca
    17221 cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac
    17281 tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc
    17341 accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac
    17401 gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg
    17461 gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa aataaactcc
    17521 tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc
    17581 atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta
    17641 aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcac
    17701 tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc aactggtttg
    17761 caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt
    17821 ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca cacgccatct
    17881 tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa cagcatcgtt
    17941 ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc ttccatccgc
    18001 atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg
    18061 atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg actacggtcg
    18121 ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc tgcataactg
    18181 aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc
    18241 aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc
    18301 tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag
    18361 gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg
    18421 ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc
    18481 gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac aaaacgccgc
    18541 tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga
    18601 ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg
    18661 gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc
    18721 agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc ttgatagaac
    18781 gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg agaagaccgc
    18841 cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat caccagcgca
    18901 ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc catgtgcgac
    18961 gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt tgcaccgagg
    19021 ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc catcatgcgc
    19081 caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag aatcgccagc
    19141 atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc catcgccagg
    19201 aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac gcggtaaaag
    19261 cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca gacgaaggtc
    19321 ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac cgtcaggcag
    19381 atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg tcccacatcc
    19441 ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg cgtggtggag
    19501 tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac cagaccgaac
    19561 gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc aatgaacaat
    19621 ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc ggccaacagc
    19681 cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc attttgcatc
    19741 accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc ggcacgcaaa
    19801 ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa cggatcgcgc
    19861 agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag tagcgccagc
    19921 gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa aaaggagacg
    19981 gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag cgttgctaaa
    20041 gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat actcgtcata
    20101 cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg ctttcctggg
    20161 tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa ataccaaaag
    20221 ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg gcaaacgttt
    20281 gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg ttttcgatgt
    20341 ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa aacagcgaac
    20401 tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc gcctgtcagc
    20461 atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc caggcgctgg
    20521 gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc tccggtgtgg
    20581 catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg tctattcagg
    20641 gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg aaaaccggtg
    20701 gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg gcaagttgca
    20761 ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg ccgctggttt
    20821 ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg acctccattg
    20881 gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat ctgctgctgg
    20941 caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct tacttacgcg
    21001 tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt atgggcatgt
    21061 tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg ccgctctatt
    21121 acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt atgatcactt
    21181 cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag ccagtgtccg
    21241 gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg aactcgtttg
    21301 tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac ggagtgatcc
    21361 agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg ttgatcgtgc
    21421 tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg gttctgggcg
    21481 gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc gtttctcgtg
    21541 agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt ggtctgggcg
    21601 tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac ctgctctcct
    21661 ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc ccaccagaaa
    21721 aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct ctttcaccat
    21781 tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat ttcacgggat
    21841 ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct ctgttagtgg
    21901 tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat atcagcgcat
    21961 gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac cgtttttccg
    22021 cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag cccgcgcccc
    22081 tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa ccacgccatg
    22141 tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc gcgccgctac
    22201 cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg aatagcgaat
    22261 ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca gaagccggta
    22321 aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg ctcaatgatg
    22381 ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt acgctgacta
    22441 acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt aacgccgacg
    22501 gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc gaaaaatcgc
    22561 taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt cgcttggaag
    22621 tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat ctcagcttgc
    22681 gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg tcgatgaacg
    22741 ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac gcggaatttt
    22801 ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt atggtcagaa
    22861 cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg gcaattgccg
    22921 ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg acacccggtt
    22981 ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc attgatatcg
    23041 accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac ctgacgctgg
    23101 ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc gccgccgcga
    23161 cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac agcagcacgg
    23221 tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc accgcaagtg
    23281 tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt gtgccggtga
    23341 atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc aatattcagc
    23401 ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt agcgggcaat
    23461 tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg ggcgtcgttt
    23521 ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg ccgggtacga
    23581 ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg cattcccttg
    23641 aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca gaccatttac
    23701 tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac cagtcacagt
    23761 aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt cagcagcagg
    23821 cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg ccgtgccttc
    23881 gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag tgttatcacg
    23941 cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac taccgtcgag
    24001 ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact gcacatcgcc
    24061 cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt gacggtcaag
    24121 gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca tcggcgtacc
    24181 ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc atttcagttg
    24241 cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca cacgataaga
    24301 tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc agcgtttgta
    24361 aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga agccaaaacc
    24421 tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt agttagcgta
    24481 acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg agcgggcaaa
    24541 caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca gttcgttgta
    24601 gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc actgaacatc
    24661 aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg cgaccagacc
    24721 tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat aaatcgccag
    24781 acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga gtaaatagcc
    24841 tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt taatccagac
    24901 gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt cagggaaagt
    24961 cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac agtcaaagtg
    25021 gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt ttaccgtcgc
    25081 ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc acgggggcgg
    25141 cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg gcgtcgggcc
    25201 gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt tggagacttt
    25261 ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc cataaccacg
    25321 gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc cgccgtcccg
    25381 gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct ctcccagacc
    25441 gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc gttgattatt
    25501 cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt cgccgttgcg
    25561 cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt tgccactttt
    25621 aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca aaatattgag
    25681 cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca caccgacaat
    25741 accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct gccaggtacg
    25801 ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct gttcaacctc
    25861 gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt ttccatcgct
    25921 aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg
    25981 gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg tgagtgagta
    26041 gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa tcgtgaatag
    26101 cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat catatccgcc
    26161 ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt tgagcacaaa
    26221 cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact cgccgtagtc
    26281 gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca acacaccaat
    26341 cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga acatcgcgag
    26401 gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac atttccagtc
    26461 ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag tggtgagaaa
    26521 agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca tcgccccacc
    26581 gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc gccactggtc
    26641 gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg gtgcttcaac
    26701 gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca cggaaaggac
    26761 cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat taaccagtgg
    26821 catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac gccaggattg
    26881 cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc agtaagggat
    26941 attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca tagatcattt
    27001 tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc ccgaatggca
    27061 gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt cgatcggcca
    27121 acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa aacatggttc
    27181 cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata tataacatta
    27241 cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt ttctctttaa
    27301 cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc tgagaccgat
    27361 aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt tattaattta
    27421 tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca ttgtaaaata
    27481 tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt aggcggaaaa
    27541 aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc gcctccatgt
    27601 agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc aaatccagtt
    27661 ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag tttacctctt
    27721 ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt atttatttgt
    27781 atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac tttagggggt
    27841 gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata cataacaacg
    27901 gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt tagttactgg
    27961 cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat ggcattaacg
    28021 atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct gatggcggcg
    28081 gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag taccgctttg
    28141 ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta gccgatgctc
    28201 gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca ggggaggcaa
    28261 aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag gctattgctc
    28321 gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat gaaatcattg
    28381 atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc gaaatcaaac
    28441 caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg gagaaaatgc
    28501 gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact ggccgggcag
    28561 actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa gtacattttc
    28621 cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt tataccggaa
    28681 gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga acacaagaac
    28741 tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag atcccggcag
    28801 aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc attatgctat
    28861 ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt agaaatgatc
    28921 cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt ggatataagg
    28981 gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat gaacaaggct
    29041 ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc atcaggggta
    29101 cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg tacgctgatt
    29161 acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc tgatgttttc
    29221 ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt attatattat
    29281 ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga catttttaaa
    29341 ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa ggaatagttg
    29401 attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg agattcacat
    29461 aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca atattcattc
    29521 ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag actatatcac
    29581 cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg cacgataaca
    29641 agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag tttgaagaag
    29701 tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac aaactccgac
    29761 gccttgctca acgggagccg gactttattg atatacacgc tcatctggca tatgcatttc
    29821 tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg gcggcaggta
    29881 atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc gaaaaccggc
    29941 cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag cgccatcagg
    30001 atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat cagggggctc
    30061 gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc ttcagtgtac
    30121 tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc ctgcattttc
    30181 tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc acaaacactt
    30241 acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg tggcatgact
    30301 tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca ttatggggac
    30361 aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc gtgctgcatg
    30421 aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat ttcgaaatat
    30481 gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag acactttctg
    30541 aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc tggatattgc
    30601 ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata ttgcattagt
    30661 gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc cttccccagg
    30721 tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg ttgtaaatta
    30781 ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta tcgtcaaaca
    30841 cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc ccctcctctg
    30901 gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa acagtaaaaa
    30961 attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg cttgtgttct
    31021 gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg catccaactt
    31081 ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat caatggattt
    31141 cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc atactggcgc
    31201 aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga cgcccaaaat
    31261 ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt caataaattt
    31321 tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc aaaagtttat
    31381 ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat tgttacaccg
    31441 atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc agcgacctcc
    31501 attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg tgagattggg
    31561 cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg gacacggccc
    31621 taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc actgtgacga
    31681 cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat tatttcccgg
    31741 ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa gctttccaca
    31801 caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt gtgtcgcttc
    31861 agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg aacaatgacg
    31921 ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc cagttgtgct
    31981 gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc gatgactact
    32041 gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc ccacttctgg
    32101 tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg gcaggccatg
    32161 tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc cctgacgacg
    32221 caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa ggcggggagc
    32281 accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc aggtgagccg
    32341 aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc agccgctgcg
    32401 ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat gattttcgat
    32461 aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg gaggatggac
    32521 agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc ggccagttcg
    32581 ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc ataaagctga
    32641 gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttt agcgagtttc
    32701 agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct gattgatact
    32761 ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt gtccactatt
    32821 ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat atcatctgta
    32881 agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta taactggaat
    32941 gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc accaaagata
    33001 actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac caaagagttt
    33061 atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta catatcaata
    33121 tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc aattattttc
    33181 tccttctcag ttttattttt cataatcgga aatcaattat caaagattga aaaattatcc
    33241 aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa atggttcaaa
    33301 aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg gtacaatcag
    33361 tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt taggtcttgg
    33421 ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa ataataaaaa
    33481 gtaagtgtac tctgactgcg tatgtatcta cttgaaccgc cccgg
//
LOCUS       DAJTOW010000036        32721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTOW010000036 DAJTOW010000000
VERSION     DAJTOW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.036"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(2..94)
                     /locus_tag="OO244_001822"
     tRNA            complement(2..94)
                     /locus_tag="OO244_001822"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO244_001823"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO244_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5663671.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO244_001824"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO244_001824"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5663672.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO244_001825"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO244_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5663673.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO244_001826"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO244_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5663674.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO244_001827"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO244_001827"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5663675.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO244_001828"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO244_001828"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5663676.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO244_001829"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO244_001829"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO244_001830"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO244_001830"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5663677.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO244_001831"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO244_001831"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5663678.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO244_001832"
                     /pseudo
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO244_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO244_001833"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO244_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5663679.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO244_001834"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO244_001834"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5663680.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO244_001835"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO244_001835"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5663681.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO244_001836"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO244_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5663682.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO244_001837"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO244_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5663683.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO244_001838"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO244_001838"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5663684.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO244_001839"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO244_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5663685.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO244_001840"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO244_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5663686.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO244_001841"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO244_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5663687.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO244_001842"
     CDS             complement(20900..21019)
                     /locus_tag="OO244_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5663688.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO244_001843"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO244_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5663689.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO244_001844"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO244_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5663690.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTSSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25475)
                     /gene="hycC"
                     /locus_tag="OO244_001845"
     CDS             complement(23682..25475)
                     /gene="hycC"
                     /locus_tag="OO244_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5663691.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGS
                     VWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSN
                     SGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVS
                     VVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMA
                     ICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVP
                     GWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25512..26123)
                     /gene="hycB"
                     /locus_tag="OO244_001846"
     CDS             complement(25512..26123)
                     /gene="hycB"
                     /locus_tag="OO244_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5663692.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26248..26709)
                     /gene="hycA"
                     /locus_tag="OO244_001847"
     CDS             complement(26248..26709)
                     /gene="hycA"
                     /locus_tag="OO244_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5663693.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26921..27271
                     /gene="hypA"
                     /locus_tag="OO244_001848"
     CDS             26921..27271
                     /gene="hypA"
                     /locus_tag="OO244_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5663694.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27275..28147
                     /gene="hypB"
                     /locus_tag="OO244_001849"
     CDS             27275..28147
                     /gene="hypB"
                     /locus_tag="OO244_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5663695.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28138..28410
                     /gene="hypC"
                     /locus_tag="OO244_001850"
     CDS             28138..28410
                     /gene="hypC"
                     /locus_tag="OO244_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5663696.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28410..29531
                     /gene="hypD"
                     /locus_tag="OO244_001851"
     CDS             28410..29531
                     /gene="hypD"
                     /locus_tag="OO244_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5663697.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29528..30538
                     /gene="hypE"
                     /locus_tag="OO244_001852"
     CDS             29528..30538
                     /gene="hypE"
                     /locus_tag="OO244_001852"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5663698.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30612..32690
                     /gene="flhA"
                     /locus_tag="OO244_001853"
     CDS             30612..32690
                     /gene="flhA"
                     /locus_tag="OO244_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5663699.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
BASE COUNT         7716 a         9113 c         8536 g         7356 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc taggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctactggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag caggataatg ccgatatttt ccagggtgtg gtaagccagc
    24661 aggcgctgga tgttgtgttc catcaacgca tacagaccac cgacgaacgc ggtgatcatg
    24721 ccgagcacca gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg
    24781 gttaacatgc ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc
    24841 gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca gcggaataat cccagccagc
    24901 aggccaaagc caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata
    24961 cgcatatcca gcaggcacag atccagcgtg ccataacgct gccacagcag ccagcaagca
    25021 atcaccagca gcagagtgcc aagacgcctc agcgcaaacc acagtttgcc ctctttgctg
    25081 ttgctggtga ggaacaccgc acacaaggcc atgacttcgg ccattaccac gaacatgccg
    25141 aggttgctgg cgatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg
    25201 ttgcacttca cctgcgcgtg gcgatgccag tcaatgttat agaggctgac aaacagaccg
    25261 caaaggccga gggtaatcag ccaaatcgcg ttaagcgggg agatttgcac atcgtagctt
    25321 accagcgaca gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa gcccgcggct
    25381 gccgtataca gactaccaac cgcgccgcca attccggcta tccagccact tagcgctttt
    25441 tgaaaagaga aatgccagaa cagcggcggc gacaaaccat gccacgccgc tattgatcag
    25501 ggaaattgcg ctcatttagc ctctccactt tgagcctgct gaaacaaggt gagatcgcca
    25561 aagtccgtgt taaaggtcag ctcacgctta cgtttgctga cgcgggcgat atcggtgtta
    25621 tccaccagat gcagggcttt agtcgggcac atccgcacac aggccggacc ttgttcatca
    25681 aagctacaaa ggtcacattt gacggcgatc gcgcgaatac ctggcaccca gtcaagcaat
    25741 gtgctgacac gcgccggagc aggcggtgcc ggtggcgctt tcggggtatt ggcgtttgcc
    25801 ggaatatcca gcggacggct gccggaaaat tcaattgcgc caaacgggca ggcgatgccg
    25861 cacagtttgc agcttacgca cagactttca ttcaactgca cggccccatc aacgcgggtg
    25921 atggcgttaa ccgggcagac caccgcgcag ggtgcatctt cacagtggtg acagagctgc
    25981 ggcgcagatt ctttttcatt gagcatcact ctcaggcgcg gcattgattg caggccgtgc
    26041 tggcgatgcg tctctgaaca ggcggcctca caggtgtggc agccgataca gagcgtggag
    26101 tcggcaatta caaaacgatt caccaggcat tcctcaggtg attgtcattt ttgacgaaaa
    26161 catgccgatg aaatgtcatt ttcgacactc atcgacacgc ccatccccaa acaggcgtaa
    26221 cgcctgcaaa acgggcaaag cctcagctca tgctgccggg ctttgtccct ttaccagttg
    26281 gcttaaattc accggcacat tgttttcaat ggcggtgtat aagctgtcgt aaacgccagc
    26341 gattttttcg agatagtgtt ccagaacctg ttcgcgatcg cggatgttga ccttgccatc
    26401 aatcatgccg tcaatgctca gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt
    26461 tgtttcgacg taatactcga tggtgtggct gttaaagcca tccgccaggg tgacgtaaat
    26521 acgaaaatgt tcaaaaagga cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg
    26581 atgcaggcgc gctttcgata acgtgatccc ctgaaccagc gaattgcagt agatgtgcca
    26641 ctggtcctgt aggcgacgat gccgctgtgc gatgtaatcg gctttctcgc ttatttccca
    26701 aatagtcatt gtcaggttac tcgtttaaca gagatgccag ctttaagcat tttttgtgcc
    26761 aacttttaat ttattgttat taaagagatt tttaagctaa agatgaattt cgtcgccgtg
    26821 tcgacgtgtc atttcgacat catcgacatt attcaccgca gggataatca acactggcgc
    26881 aattattgct tgtagctggc aatagttaat gggaggcgat atgcacgaaa taaccctctg
    26941 ccaacgggca ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac
    27001 tggggtctgg ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc ttgccttttg
    27061 ttttgatctg gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca
    27121 agaggccgaa tgctggtgtg aaacatgcca acagtatgtg acgctactga cccagcgcgt
    27181 ccgccgctgt ccacagtgtc atggtgacat gctacagatt gtggcagacg acggtttaca
    27241 gattcggcgg atagaaatag accaggagtg agcgatgtgt acaacatgcg gttgcggtga
    27301 aggcaacctg tatatcgagg gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc
    27361 atttgccccg gcggcacgcc cgcagatgaa aatcaccggc attaaagcgc ctgaatttac
    27421 ccccagccag actgaagaag gcgacctgca ttacggtcat ggcgaagcgg gcactcacac
    27481 accgggcatg agccagcgtc ggatgctgga agtcgaaatt gacgtgctgg acaaaaacaa
    27541 ccgtctggct gaacgcaacc gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct
    27601 ggtttccagc cctggttccg gtaaaaccac cctgctgacg gaaaccttaa tgcgcctgaa
    27661 agacagcgtt ccgtgcgcgg ttattgaagg cgaccagcaa accgtgaacg atgccgcacg
    27721 cattcgcgct accggtacac cagcgattca ggtgaacacc ggtaaaggct gccatcttga
    27781 cgcacagatg attgccgacg ccgcaccgcg tctgccactg gacgataacg gtattctgtt
    27841 tatcgaaaac gttggcaacc tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa
    27901 agtagcggtg ctttccgtta ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt
    27961 tgccgccgcc tcgctgatgc tgctcaacaa agttgacctg ttgccgtatc tcaactttga
    28021 cgttgagaag tgcatcgcct gcgcccgcga agtcaatcca gaaattgaaa tcatccttat
    28081 ttccgccacc agcggcgaag ggatggacca gtggctgaac tggctggaga cacagcgatg
    28141 tgcataggcg ttcccggcca gatccgcacc attgacggca accaggcgaa agtcgacgtc
    28201 tgcggcattc agcgcgatgt cgatttaacg ttagtcggca gctgcgatga aaacggtcag
    28261 ccgcgcgtgg gccagtgggt actggttcac gttggctttg ccatgagcgt aattaatgaa
    28321 gccgaagcac gcgacactct cgacgcctta caaaacatgt ttgacgttga gccggatgtc
    28381 ggcgcgctgt tgtatggcga ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg
    28441 aacaggtgat gcagttaatt gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg
    28501 ccgaacgccc tctgcggatt atggaagtgt gtggcggtca tacccacgcc atttttaaat
    28561 tcggcctcga ccagttactg cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg
    28621 tgtgcgtact gccgatgggt agaatcgaca cctgcgtgga gattgccagc catccggaag
    28681 tcatcttctg tacctttggc gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc
    28741 aggcaaaagc acgcggtgcc gatgtgcgca tcgtttactc gccgatggat gcgttgaaac
    28801 tggcgcagga gaatccaacc cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca
    28861 tgccgaccac cgccatcact ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact
    28921 tcttctgcca gcacattacg cttatcccga cgctgcgcag tttgctggaa cagccggata
    28981 acggtatcga cgcgttcctc gcgccgggcc acgttagtat ggttatcggc actgatgcct
    29041 ataattttat cgccagcgat tttcatcgtc cgctggtggt cgctggtttc gaaccccttg
    29101 atctactgca aggcgtggtc atgctggtgg agcagaaaat agcggcccac agcaaggtag
    29161 agaatcagta tcgtcgggtg gtaccggatg ccggtaacct gctggcgcaa caggcgattg
    29221 ccgatgtgtt ctgtgtcaac ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt
    29281 ctggcgtgca cctgacgccg gattatcaac gattcgatgc cgaagcacat ttccgcccgg
    29341 caccgcagca ggtctgcgat gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat
    29401 gtaagccgca tcaatgcccg ctgtttggta acacctgtaa tcctcaaacc gcatttggtg
    29461 cactgatggt ctcctccgaa ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga
    29521 gtgaagcgtg aataatatcc aactcgccca cggcagcggc ggccaggcga tgcagcaatt
    29581 aatcaacagc ctgtttatgg aagcctttgc caacccgtgg ctggcagagc aggaagatca
    29641 ggcacgtctt gatctggcgc agctggtagc ggaaggcgac cgtctggcgt tctccaccga
    29701 cagttacgtc attgacccgc tgttctttcc tggcggtaat atcggcaagc tggcgatttg
    29761 cggcaccgcg aatgacgttg cgggcagtgg cgctattccg cgctatctct cctgtggctt
    29821 tatcctcgaa gaaggattgc cgatggagac actgaaagcc gtagtgacca gcatggcaga
    29881 aaccgcccgc acggcaggca ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg
    29941 cgcggcagat aaactgttta tcaacaccgc tggcatgggc gcaattccgg cgaatattca
    30001 ctggggcgca cagacgctaa ccgcaggcga tgtattgctg gttagcggta cactcggcga
    30061 ccacggggcg actatcctta acctgcgtga gcagctgggg ctggatggcg aactggtcag
    30121 cgactgcgcg gtgctgacgc cgcttattca gacgctgcgt gacattcccg gcgtgaaagc
    30181 gctgcgtgat gccacccgtg gtggtgtaaa cgcggtggtt catgagttcg cggcagcctg
    30241 cggttgtggt attgaaattt ctgaatcagc gctgccggtt aaacctgccg tgcgcggcgt
    30301 ttgcgaattg ctgggactgg acgccctgaa ctttgccaac gaaggcaaac tggtgatcgc
    30361 cgttgaacgc aacgcggcag agcaagtgct ggcagcgtta cattcccatc cactggggaa
    30421 agacgcggcg ctgattggtg aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta
    30481 tggcgtgaaa cgaaccctcg atttaccaca cgccgaaccg cttccgcgta tatgctaata
    30541 aaattctaaa tctcctatag ttagtcaatg accttttgca ccgctttgcg gtgctttcct
    30601 ggaagaacaa aatgtcatat acaccgatga gtgatctcgg acagcaaggg ttgttcgaca
    30661 tcactcggac actattgcag cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac
    30721 tggtaaagcg ttctgcgctc gccgacaacg cggctattgt gttgtggcaa gcgcagactc
    30781 aacgtgcgtc ttattacgcg tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa
    30841 ctgttctggc acacggtccg gtacgcagca ttttgtcgcg ccctgatacg ctgcattgca
    30901 gttacgaaga attttgtgaa acctggccgc agctggccgc aggtgggcta taccccaaat
    30961 ttggtcacta ttgcctgatg ccactggcgg cgggagggca tatttttggt ggctgtgaat
    31021 ttattcgtta tgacgagcgt ccctggagcg aaaaagagtt caatcgtctg caaacattta
    31081 cgcagatcgt ttctgtcgtc accgaacaaa tccagagccg cgtcgtcaac aatgtcgact
    31141 atgagttgtt atgccgggag cgcgataact tccgcatcct ggttgccatt accaacgcgg
    31201 tgctttcccg cctggatatg gacgaactgg tcagcgaagt cgccaaagaa atccattact
    31261 atttcgacat tgacgatatc agcattgtct tacgcagcca ccgtaaaaac aaactcaaca
    31321 tctactccac tcactatctt gataaacaac atcccgccca cgaacagagc gaagtcgatg
    31381 aagccggaac cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg atcaatctct
    31441 acgagcggga cgatttagcc ccctatgaac gcatgttgtt cgacacctgg ggcaaccaga
    31501 ttcaaacctt gtgcctgtta ccgctgatgt ctggcgacac catgctgggc gtgctgaaac
    31561 tggcgcaatg cgaagagaaa gtgtttacca ctaccaatct gaatttgctg cgccagattg
    31621 ccgaacgtgt ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc catcgtctga
    31681 aagaacggct ggttgatgaa aacctcgccc tgaccgagca gctcaacaat gttgatagtg
    31741 aatttggcga gattattggc cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa
    31801 tggtggcgca aagtgacagt accgtgctga tcctcggtga aactggctcg ggtaaagagc
    31861 tgattgcccg tgcgatccat aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga
    31921 actgcgcggc gatgcctgcc ggattgctgg aaagcgatct gtttggtcat gagcgtgggg
    31981 cttttaccgg tgccaacgcc cagcgcattg gtcgttttga actggcggat aaaagctctc
    32041 tgttcctcga cgaagtgggc gatatgccac tggagttaca gccgaagttg ctgcgtgtat
    32101 tgcaggaaca ggagtttgaa cgtctcggca gcaacaaaat cattcagacg gacgtacgtt
    32161 taattgccgc gactaaccgc gatctgaaaa aaatggtcgc cgaccgagag ttccgtagcg
    32221 atctctatta ccgcctgaac gtattcccga ttcacctgcc gccactacgc gagcgtccgg
    32281 aagatattcc gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca
    32341 atatcgacag cattcctgcc gagacgctgc gcaccttgag caacatggag tggccgggta
    32401 acgtacgcga actggaaaac gtcattgagc gcgcagtatt gctaacacgc ggcaacgtgc
    32461 tgcagctgtc attgccagat attgctttac cggagcctga aacgccgcct gccgcaacgg
    32521 ttgtcgccca ggagggcgaa gatgaatatc agttgattgt gcgcgtgctg aaagaaacca
    32581 acagcgtggt tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc
    32641 tgctgtcacg gatgaagcgg ctgggaattg ataaatcggc attgatttaa ctgcaaattg
    32701 ccggacagat ctgcctgtcc g
//
LOCUS       DAJTOW010000037        32248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTOW010000037 DAJTOW010000000
VERSION     DAJTOW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.037"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            86..229
                     /locus_tag="OO244_001854"
     CDS             86..229
                     /locus_tag="OO244_001854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663700.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(448..1986)
                     /gene="aldB"
                     /locus_tag="OO244_001855"
     CDS             complement(448..1986)
                     /gene="aldB"
                     /locus_tag="OO244_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5663701.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2151..3302)
                     /gene="yiaY"
                     /locus_tag="OO244_001856"
     CDS             complement(2151..3302)
                     /gene="yiaY"
                     /locus_tag="OO244_001856"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5663702.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3492..5336)
                     /gene="selB"
                     /locus_tag="OO244_001857"
     CDS             complement(3492..5336)
                     /gene="selB"
                     /locus_tag="OO244_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5663703.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5333..6724)
                     /gene="selA"
                     /locus_tag="OO244_001858"
     CDS             complement(5333..6724)
                     /gene="selA"
                     /locus_tag="OO244_001858"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5663704.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6822..7430)
                     /locus_tag="OO244_001859"
     CDS             complement(6822..7430)
                     /locus_tag="OO244_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5663705.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7501..8586)
                     /locus_tag="OO244_001860"
     CDS             complement(7501..8586)
                     /locus_tag="OO244_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5663706.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8589..8951)
                     /locus_tag="OO244_001861"
     CDS             complement(8589..8951)
                     /locus_tag="OO244_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5663707.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9488..11401
                     /gene="mtlA"
                     /locus_tag="OO244_001862"
     CDS             9488..11401
                     /gene="mtlA"
                     /locus_tag="OO244_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5663708.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11496..12644
                     /gene="mtlD"
                     /locus_tag="OO244_001863"
     CDS             11496..12644
                     /gene="mtlD"
                     /locus_tag="OO244_001863"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5663709.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12644..13231
                     /gene="mtlR"
                     /locus_tag="OO244_001864"
     CDS             12644..13231
                     /gene="mtlR"
                     /locus_tag="OO244_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5663710.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13243..13452)
                     /locus_tag="OO244_001865"
     CDS             complement(13243..13452)
                     /locus_tag="OO244_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663711.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13736..14098
                     /locus_tag="OO244_001866"
     CDS             13736..14098
                     /locus_tag="OO244_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5663712.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            14642..15325
                     /locus_tag="OO244_001867"
     CDS             14642..15325
                     /locus_tag="OO244_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5663713.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            15369..20031
                     /locus_tag="OO244_001868"
                     /pseudo
     CDS             15369..20031
                     /locus_tag="OO244_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            20399..22054
                     /gene="lldP"
                     /locus_tag="OO244_001869"
     CDS             20399..22054
                     /gene="lldP"
                     /locus_tag="OO244_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5663714.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            22054..22830
                     /gene="lldR"
                     /locus_tag="OO244_001870"
     CDS             22054..22830
                     /gene="lldR"
                     /locus_tag="OO244_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5663715.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            22827..24017
                     /gene="lldD"
                     /locus_tag="OO244_001871"
     CDS             22827..24017
                     /gene="lldD"
                     /locus_tag="OO244_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5663716.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            24213..24686
                     /gene="trmL"
                     /locus_tag="OO244_001872"
     CDS             24213..24686
                     /gene="trmL"
                     /locus_tag="OO244_001872"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5663717.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(24739..25560)
                     /gene="cysE"
                     /locus_tag="OO244_001873"
     CDS             complement(24739..25560)
                     /gene="cysE"
                     /locus_tag="OO244_001873"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5663718.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(25640..26659)
                     /gene="gpsA"
                     /locus_tag="OO244_001874"
     CDS             complement(25640..26659)
                     /gene="gpsA"
                     /locus_tag="OO244_001874"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5663719.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(26659..27126)
                     /gene="secB"
                     /locus_tag="OO244_001875"
     CDS             complement(26659..27126)
                     /gene="secB"
                     /locus_tag="OO244_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5663720.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(27189..27440)
                     /gene="grxC"
                     /locus_tag="OO244_001876"
     CDS             complement(27189..27440)
                     /gene="grxC"
                     /locus_tag="OO244_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5663721.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(27582..28013)
                     /locus_tag="OO244_001877"
     CDS             complement(27582..28013)
                     /locus_tag="OO244_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5663722.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            28258..29802
                     /gene="gpmM"
                     /locus_tag="OO244_001878"
                     /gene_synonym="pgmI"
     CDS             28258..29802
                     /gene="gpmM"
                     /locus_tag="OO244_001878"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5663723.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            29812..31095
                     /gene="envC"
                     /locus_tag="OO244_001879"
     CDS             29812..31095
                     /gene="envC"
                     /locus_tag="OO244_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5663724.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            31099..32058
                     /locus_tag="OO244_001880"
     CDS             31099..32058
                     /locus_tag="OO244_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5663725.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(32045..32248)
                     /locus_tag="OO244_001881"
                     /pseudo
     CDS             complement(32045..32248)
                     /locus_tag="OO244_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         7982 a         8469 c         8264 g         7533 t
ORIGIN      
        1 ttatatactt tctgaaatgt gctgtctggt ttttcaacag gacgaggttt atcatattga
       61 aatagtggaa ctattggcca aactaatgaa taactccagt aaaacatcca cagtacagat
      121 taagcgtatt aaaccttcaa ttatctaccg tttattgctg attggcctcg gatcaccaat
      181 ggtgatttac ggcctggttc gcccgctcac catcgaaacg cgagattaaa ctcctgacaa
      241 aacacgcccc agaaacgcac ttctgttggc gtagtctggg ttattgcgca cgtaggttta
      301 aaacgtaaat tgtaacaacg tgcgctttgt ttatgccgga tgcggcgtgt acgccttatc
      361 cggcctacgg tcaggttccc gtaggcatga taagacgcgt aagcgtcgca tcaggcaatg
      421 aatacccaat gcgaccagct tcttatatca gaacagcccc aacggtttat ccgagtagct
      481 caccagcagg cacttggttt gctggtaatg ctccagcatc atcttgtggg tttcgcgacc
      541 gatacctgat tgtttgtagc caccaaacgc agcatgtgcc gggtaagcgt gataacagtt
      601 ggtccacacg cgcccagcct gtatgccgcg ccccatctta taggccagat taccgttgcg
      661 gctccagacg cccgcgccca ggccatattg cgtatcgttc gccagctcca gcgcttcttc
      721 catcgttttg aaggtggtca ccgccagcac cgggccaaaa atctcctcct ggaacacccg
      781 catattgttc tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt tcagttcacc
      841 ttccagcagc ttgcgccgcc cgcctgtgag cacgtcagcg ccctcttttt taccgatatc
      901 aatgtagttg aggatggttt ccagttgccc gtgagaaacc tgcgcgccca tttgcgtcac
      961 gctgtcgagc gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc gttccataaa
     1021 gcgttcgtag attgactcct gcactaaggc acgactcgga caggtgcaaa cttcgccctg
     1081 gttaaaggca aacagtgcaa agccttccag cgctttatcg aaaaaggcat cttcttcatc
     1141 catcacatca gcaaagaaga tatttggcga cttaccgccc aactccagcg tcaccggaat
     1201 aatgttttgc gttgcgtatt gcataatttg ttggcccact tccgttgagc cggtaaacgc
     1261 cactttggcg atgcgtttcg aggtcgccag atattcgcca atttccccac ctgcgccatt
     1321 gaccacgttc accacgcccg gcggcagtaa atcaccgaca atttccatta gcagcagtac
     1381 agaaagcggg gtaagacgtg cgggtttcag caccacacag ttgcccgccg ccagcgcggg
     1441 agccattttc cagctcgcca tcagcagcgg gaagttccac gggataatct gccccaccac
     1501 gcctaacggt tcatggaaat gataggccac ggtttcgcta tcaacttcac tgatcccacc
     1561 ttcctgcgcc cgaatacacg aggcgaaata gcggaaatgg tcaatcgcca gcggtacatc
     1621 cgcagcactg gtttcgcgaa tgggtttgcc gttatcccag gtttcagctg tcgctaacag
     1681 ctcgaggttt tgttccattc gatcggcaat cttaaacaga atcgccgcgc gatcctgcac
     1741 cgaggtgtgc gcccatttat ctttcacttt gtgcgcagca tccagcgcca gatcgatgtc
     1801 tcgtttgccc gaagacgcca cttcgcacag cagctgcccg gtcaccggcg tcagattctg
     1861 gtaatactca ccatcggcag gggctaccca ttcaccgcca ataaagttgt cgtagcgggc
     1921 ttttaacttg atggggaaac catactcgcc gggcttaatc tgtgctgaag ggggattatt
     1981 ggtcatgatc gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac tggcgaaatc
     2041 ttcgccagtc ggtaacagct ttacgacagc tatcacgaat ttacgggcaa ggattacaga
     2101 gatagttgaa aaagcggctg acaatttgcc agccgttgtt ggaatgatga ttacattgcg
     2161 gcgcgataaa tcaccacaat ttcttcgtga gttgcctgga tcgggttagt aaagccacag
     2221 gcatctttca gggcattagt cgccagaacc gcgaaatctt cttctttcac gttcaggtcg
     2281 cgtaggcctg ccgggatatc cactttcttc gccagttcac ggatggcgtt aatgcaggct
     2341 tcagcacctt ccgcgtcgtt tttacctgtc acgttcacgc ccattgcagc ggcacagtca
     2401 cgcagacgtg cagcggcgac tttgctgttg aatacctgaa catgcggcag cagaacggcg
     2461 ttacatacac cgtgtggcag gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca
     2521 taacccagag aagcattatt gaacgccata ccggcaagga actgggcata agccattgct
     2581 tcacgcgctt tcgcattact gccatcttca acggctaacg gcaggttttc ggcaatcatg
     2641 gtcacggctt tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga aacatacgct
     2701 tcgatagcgt gcgttaaggc atccataccc gttgcggcgg tcagtgactt cggcatacca
     2761 atcatcagag aggagtcatt gacagaaagc agcggagtca cgtgcttatc aacaatcgcc
     2821 attttgttat gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc agaggccgta
     2881 cccgccgtgg tattgatggc gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct
     2941 tcgtaatcgc gaatatcgcc gccattggct gccaccagcg caataccttt tgcgcagtcg
     3001 tgtggagaac caccgcctaa ggagatcacg ctatcgcaat tattttcttt aagtaatttc
     3061 aaacctgcgg cgacgttttc cgtggtgggg ttaggttggg tgccatcata aataacgcta
     3121 aaaatattgc gttcttccag tgctttttgc acatcacccg ccatacctaa tttcgttaac
     3181 atattgtcag tgacaattaa ggtacgggta aatccataat ctgccatcat attcattgca
     3241 tcagtcaatg aatcagcgcc gatgacattc acagaaggaa taaagaaagt tgaagatgcc
     3301 ataacacact cctgaaagtg taaaaaaggg tgggcagctt actgaggatt ttcattcgaa
     3361 aacatgatca ttattattaa ttatcaaaat taatctaata aaaagcacat tgtattcatt
     3421 aaacaatgtg ctttatttaa aatcataaga aatttgtatt aatttatttt aaaaattaat
     3481 taatcatttc cttatttttc cggaaataat aatgcgtcgc gcaataaatg atcatttcca
     3541 cgacgacgcg taaagccaat gcggtcaaaa tattccagaa tctgaattgc cagctttcgg
     3601 cctacgccta agcgatcgcg gaaatccgcc gcgcaggttg aaccacactc ctgatcgaga
     3661 tcgcggatca tattggcaaa ttcgacaatc cgatcgttac ggtaataacg atctttgacg
     3721 atcgcggtaa ttattccttg ctgcgccgcc tggcgtagcg tcaggcgcat tgcctgctcg
     3781 tcggtacccg tctcttttgc cagatcacgc acccaccacg gctcgtcacc aaacagcggc
     3841 tctgcttttt gccagatggc ctgctgctct tcgctgaagc ccgctttgtg atccggcaga
     3901 tgcagccaac cgtgatgact gaggatgtcg ccgctttcgc gcatcttttc aatcagcaac
     3961 agtactagcg cttcatcttc cattggcaac gccatacgtc gcagacgttc gcgcccaggc
     4021 ccaggttcat cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat tttccgttgc
     4081 cagcgagcgg caaccggcgc attcaacaag ctataaccag cctgaatata gccaggctgt
     4141 tgcagcagtt cgcgcatccc ttcgccgttg agctggcgcg cccaagcgaa atcaggaaga
     4201 ttaaccgcgc cgcgttgcag gtgaacggct aacgcttcgc tatcattctg tgccctggcg
     4261 agggaagcca gccattgcag atactctggt ttacgtttac cgcgacgcgg cgggttaagc
     4321 atcacgacgc gcgctccggc cagcgtgttg cgggcagaga tatcgcgcaa taccaggcgg
     4381 tcgttatctg ccagccataa cggggtgtcg aagaccagtt cagcaaggtt atcttccagc
     4441 agtgaaacgc gtcccgtgac gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg
     4501 gtcagcggtg catgggtttg aagctcgaca atcacccgtg tgaacggctc tggcggcaca
     4561 tcggcaagca gccagtcgcc acggttaatc tgctcttttt ccgcatcacc cgcgatgtca
     4621 agcgcgatac gctgcccggc attggcggtt tctgttggct ggttttgcgc atgcagcgca
     4681 cgtacacgca tcggtttatt tacaccagtc agccagagtg aatcgcctac cttcacttcc
     4741 ccgcttaacg ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa tgcgcggtca
     4801 atcgcgaggc ggaaactatg ttggctggcg tgctcgcgtt ccggcaactg aagcagatgc
     4861 tcgcgcaggg catcaattcc ccgaccttcg gttgctgcgg tgataaacag ttttgcctca
     4921 gcaaaaccgt attcccgcag aacctccttt acctggcgtt caacctcatc aacacgcgct
     4981 tcgtccacgc gatcggcttt ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc
     5041 aaaatcgcca gatgctcacg ggtctgtgcc atcacgccgt catcgcacgc caccaccaac
     5101 agcgcgtgat cgataccacc aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg
     5161 ggaacgtcga taaaaccagg cacgcgacca tccggctgcg gccagtaggc atagccgaga
     5221 tcgatggtca tgccgcgctt tttttcttcc ggcagacggt cagcatttac gccagtaatc
     5281 gcctgcaata aggttgtttt gccgtggtca acgtgtccgg cagtcgcaat aatcatttca
     5341 acaacatctc caaaaaccgt tgctcatctt caaggcagcg taaatccagc cacaatcgtc
     5401 cgtcataaat acgaccaatc accggcactg gcaattcacg ccagcgggcg gctaatgact
     5461 caaggtggct accgcgtcca tcatggggtg taaacgttaa tgccgcgctc ggcaggcgat
     5521 caaccggcag cgaaccactg ccaatctgcg aaagacatgg cataacctgt accgcaaact
     5581 ccgcgccgta atgtgcgaca aggggagcct gtaaacgttg tgcctggatt tgaatgacct
     5641 ctgcgctgcg ggtaagcagg cgcagggtcg gtaatttttc actcagagct tcagggtgta
     5701 aataaagacg caacgtggct tccagcgccg cgagggtcat tttatccgcg cgtaatgcac
     5761 gcttcagcgg gtggctttgc aggcgggcga tcatctcttt tttaccaacg ataatcccgg
     5821 cctgcggccc gcctaacaac ttgtcgccgg agaagctcac cagactgacg cccgccgcaa
     5881 tcaactcctg cggcattggc tctttcggca aaccgtactg gctaagatcg accagcgagc
     5941 cactgcctaa atcagtcacc actggaacat ccagctcttt gccgagcgcc accagttcag
     6001 cttcgtctat cgctttggtg aacccctgaa tgctgtagtt actggtatgt actttcatca
     6061 acagtgcggt attttcattc accgcctgac gataatcatt cgcgtgcgtg cggttggtgg
     6121 tccctacttc gtgtagggtg cagcctgcct gacgcataac atcgggaata cgaaacgcgc
     6181 cgccaatctc caccagttcg ccgcgagata ccaccacctc tttcccgctg gcagtggccg
     6241 ccaacatcaa taacaccgcc gccgcattgt tattgacgat acaggcatct tccgcccccg
     6301 taatacggca cagcagctgc gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca
     6361 gatcgtactc gagggtcact ggcgaacgca tagcctgcgc aacggcttcc accgcggctt
     6421 ccgcctgtaa agctcgccca aggttggtat gcagcacggt tcccgtcagg ttgatcaccg
     6481 gacgcagcgc gctctgcgcc tctttcgtca accgggcatc gacttcttgc gcccagtttt
     6541 cacaccacgc aggcagcgtc tggctgccac gaatcacttc tcgcgcttcg tcgagcatct
     6601 gacgcaacaa ttccaccacg cgggtgtgac cataagtatc acgcaaagaa aggaaggagc
     6661 tatcgcgcaa taagcgatca atagccggaa gttgactata gagggaacgc gtttcggttg
     6721 tcataggaaa cctggctgat caaggccctc tcacacggag aagggcgttt aacataacca
     6781 cggattgtaa cgtgagatgg gtcaggagga catatcgcgc atcaagcctt tggcggttcg
     6841 gtgcgagcaa aactttcgcg gctaaacagg ttttcgacca gtttgactaa gtgtgggcgg
     6901 tcgacacacc aacctggcgc aacgcggcgg aaattgagat atccgacagc acaggcaatg
     6961 gcgatagtcg ccagattgac cgtatcggtt ttgagtgtgc catcgaccag atatccttcc
     7021 agcacatcca gactgcggtt gattttctcc cgctggcgta acaattcatc ttcagactgc
     7081 tgcgccgccg gacgcgcctg ttcacgcacc gataccagcc cggcatccat aatgccatcc
     7141 gccagcgcct caattttgcg cacccgcaac gactccagcg gatcgcgcgg caacatcgcc
     7201 ggagcgacat tcattaactc aatatattca gcgatgatcg gcgaatcaaa ccagcattcg
     7261 ccctcttcgg tcaccagcac cggcactttc cctaacgggt taaattgcgc cacgccgtta
     7321 tccgcgttat agggcagttc attaataaat tcgaaagtta tgcccttttc taacaacaga
     7381 atagaaagtt tgcgtacaaa cgggctggtg tagctaccga cgagtttcat gccgagtcct
     7441 ttgtgcgagg aaaaatatca gtatggctca ggctcacgca aaacacaggt gatataagtc
     7501 tcaatgatcc aaataaagat aatgcatcca actggtcatt cttagcagca ctttccgcat
     7561 caccgaaaca tggctgaaat ggtcggagta aaccgccacc tgggcgtaga tgccgtcggg
     7621 taaggcatcg atatcatcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc
     7681 gggaacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac
     7741 aggtaaaata ctagtcaatt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac
     7801 ttccgcatca tcgccaggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat
     7861 ttgccgtttt tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta
     7921 tgtacctggg cggatcagta cctgagtgac gtagccattg cttggtgcgc gaatgacagt
     7981 ctgctcaaga ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg
     8041 ctcgccgttg accatactgt cgagctgact ctggatctgc gcctgctccg ccaccgagcc
     8101 tttcactaac gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga
     8161 gaacggattc accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg
     8221 gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag
     8281 cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg
     8341 gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt
     8401 gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc
     8461 cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac
     8521 cagaaacacg cctcccagcg ccgccgtcgc cagcgccacg taagttaaaa caatcaatag
     8581 atccataatt agatctccgg gaacgtggaa tagtcagtct ttttctcggc ggcgagttga
     8641 tgctccagcc gggaaattcg gtcagaaagt gcggcgattt ccggttcagt cgctttctcc
     8701 tgcgacgcaa catgtgactg cataccccag ccacgctccg gttgatagag cgtcgcccag
     8761 atccacagaa acggccagat aacatgcaga gtaaacaggc tcacccagcc cgccgtatga
     8821 atagcgtcgg catgggggtg attgcgcttt ttggcaatca gataagggat gtcatgtatc
     8881 gcgatgattc cataaaaaat caccagaaag acgaagatca gcactcccaa cgcgaaatag
     8941 tttagaaaca tatctgcctc ggattcacgt ttatcagtgt tgttttttgg ctggcagcca
     9001 gaagggagtc aggctgatat tttgacaata atccgggttc gcgattctcg ccataacacc
     9061 aaagaataat ttttagaggt gatgagttgc ttagttacat aacgattgta tgacgaaggc
     9121 ataacatgct gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt gattcagatc
     9181 acaaagattc aacaaaccat caaaacaaaa atgtgacatt actcacattt aaatgccatt
     9241 tttagcgaaa atcaccgcct tgtttctttt ttatacaggc gttttgtgat gagcgtcacg
     9301 tcaattacct ctctaccccc tatatttatg tgactgatat cacacaaaag cccgtcgact
     9361 ggacagttaa ccgattcagt gccagatttc gcagtatcta caaggtccgg ctacctctgc
     9421 cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca taagaagggg
     9481 tgtttttatg tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa
     9541 catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg cgttatttat
     9601 tccaacaggg tggttaccga acgagacgct ggcgaagctg gtcgggccga tgatcactta
     9661 tctcctaccg ctgctgatcg gttataccgg tggtaagctg gtaggcggca aacgtggtgg
     9721 cgtagtcggt gccattacca ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct
     9781 cggttctatg attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg accgctgggt
     9841 agacggtaag atcaaatccg gttttgagat gctggtgaat aacttctccg caggcatcat
     9901 cgggatgatc ctcgctattc tggcattcct cggcattggc ccgattgttg aagccctgtc
     9961 caaaatgctg gctgcgggcg ttaacttcat ggttgtccat gacatgctgc cgctggcgtc
    10021 tatctttgtt gaaccggcga aaatcctgtt cctcaacaac gccattaacc acggtatctt
    10081 ctcgccgctg ggtattcagc agtcccatga actgggtaaa tcaatcttct tcctgattga
    10141 agctaaccca ggtccaggta tgggcgtgct gctggcgtac atgttctttg gtcgtggtag
    10201 cgctaaacag tctgcgggcg gtgcggcaat catccacttc ctgggtggta tccacgaaat
    10261 ctacttcccg tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat
    10321 gactggcgtg ttcacgctga ctatcctggg cggtggtctg gtttctccgg catctccggg
    10381 ttctatcctt gctgtactgg cgatgacacc aaaaggtgct tatttcgcta acatcgcggg
    10441 tgtgtgtgcg gcgatggctg tctccttcgt tgtctctgct attttgctga aaaccagcaa
    10501 agtgaaagaa gaagatgata ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga
    10561 gtctaaaggc acatctccgc tatctgcggg cgatgtgact aacgacctga gccacgtacg
    10621 taaaatcatc gttgcctgtg acgccggtat gggttcaagt gcgatgggcg caggcgtgct
    10681 gcgtaagaaa attcaggatg caggtctgtc gcagatttcg gtcactaaca gcgcgatcaa
    10741 caacctgccg ccagatgtgg acctcgtcat cactcaccgt gacctgaccg aacgcgctat
    10801 gcgccaggtt ccgcaggcac agcatatttc gctgaccaac ttcctcgaca gcggcctgta
    10861 caccagccta accgaacgtc tcgttgctgc ccagcgccat actgaaaacg aagtgaaagt
    10921 gaaagacagt ctgaaagaca gctttgacga ttccagtgct aacctgttca aactgggcgc
    10981 ggagaacatc ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg
    11041 cgagcagctg gtgaaaggcg gttacgttga gccggaatac gttcaggcga tgctggatcg
    11101 tgaaaaactg accccgactt atctgggtga gtctatcgcg gtgccacacg gtacggttga
    11161 agcgaaagat cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg
    11221 cttcggtgaa gaagaagatg acattgcccg tctggtgatt ggtattgcag cccgtaacaa
    11281 cgagcacatt caggttatca ccagcctgac caatgcactg gatgatgagt ccgtcatcga
    11341 gcgtctggca cacaccacca gcgtggatga agtgctggaa ctgctggcag gtcgtaagta
    11401 atccaatcct accctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc
    11461 cctctcgggt aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag
    11521 gtaatatcgg tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt
    11581 ttgccgatgt caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc
    11641 atgtggtcgg tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca
    11701 ttggtgatga tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc
    11761 cggttgtgct ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac
    11821 aaggtaatga atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggcaccacgc
    11881 agctgaaagg tcatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagagc
    11941 acgttggctt tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta
    12001 acgatccgct ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt
    12061 tcaaaggcgc actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg
    12121 tcgaacgtaa actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac
    12181 tggccggtca tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa
    12241 aaggtgcgat ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca
    12301 aacatgcggc gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg
    12361 atgtagagcg cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca
    12421 agccactgct cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg
    12481 ctggtgcaat gcacttccgc agtgaagatg atccgcaggc tcaggaactg gcagcactga
    12541 tcgctgacaa aggtccgcag gcggcgctgg cacagatttc cggtcttgat gccaacagcg
    12601 aggttgtatc cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca
    12661 ggacaccctg cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca
    12721 ggccgttgaa aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct
    12781 gatcaccgcc gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg
    12841 caaagacgat tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct
    12901 gggcgatctt tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga
    12961 atacgaagat gcggaactgc tgatggcatt gcgtgaagag ctaaatcacg acggcaacga
    13021 gtacgccttt accgacgacg aaatccttgg accctttggt gaactgcact gcgtggcggc
    13081 gttaccaccg ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca
    13141 gcgctatcaa caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccaa
    13201 aatcagctta aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg
    13261 cttcggccga taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc
    13321 atcgggtatt ccggacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa
    13381 cgcctggctt gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc
    13441 taatttgccc atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt
    13501 ttagtttagg ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca
    13561 tccgctgctg tttattatct ttttctatag atttcttaat taatcaacga attgcgtagt
    13621 ttttgagcat gttaatgatt acgttgcaga actattacaa tgtcatcagt tgtgaacaaa
    13681 gcacctggtc gcgcatacta ggggctataa atttatcttt atcagaagcc atcacatgaa
    13741 agaagtcgaa aaaaacgaaa tcaagcgtct cagcgatcgc ctggacgcca tccgccacca
    13801 gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg aaaaagagaa
    13861 agccacgctg gaagcagaaa ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa
    13921 agaagcacag aagctgatga agatgccgtt ccagcgcgca attaccaaaa aagagcaggc
    13981 tgatatgggc aagctgaaga aaagtgttcg cggactggtg gttgtgcacc caatgaccgc
    14041 gctggggcgc gaaatgggcc tggaagagat gactggattt tctaagacta cgttttaagc
    14101 ccctcagacg actggttcga tcgtatcaac ccgcatctgg cttgccttag ctggtcaatg
    14161 cggggactct cctgtctact cgcctaatta ccaaacaatt cactcccttt acttcgattt
    14221 accgctggtt gatattctga aaatgcattt ttaccgaaat agccatctat aaacaataaa
    14281 aatatataga agatatcatt tgttaattaa caagcattta cgtctctttt tttcggattt
    14341 aattgtgaaa ttttaatcat catattcaca aaatcaatta tctgtgaaat ttttcccaga
    14401 catggtttct ttcatatttg acatttatta ataatcatca ttagaatcca cgaaaaataa
    14461 tatattcata caaacacaga catcttttga aacgaatgat acatttttat gatgaatgct
    14521 taaaacaata tttaatcgac acacatcact gtcattaatg taaagcaaca tagctcacca
    14581 ataatacaat ggacaactct accggcattg agcgctggtc ggaggaaata aggattttta
    14641 catgaacttg aagaagattt ttttcagtgc tgtcactgtt tcagttctct gcgcattaac
    14701 gggatgtgac tacattgaag aaggcaagcc agaaagtagt ttactgaaac aacaagaaga
    14761 acataataat aaaattgtgc tacttgaaaa acaacaagca cagctaaaga gtcaactcga
    14821 aactattcaa aaacaacaaa ctggcataat aaacagtaca aagactttaa ctcacgtaat
    14881 taaatccgtt aaagaccaac aaaatacttt tatttttacc gagtttaacc cagcaaaaac
    14941 gaagtatttc atcctgaaca acggttcggt ggctttagcg ggtcgagtgt tatccattga
    15001 cgccacagag aatggtagtg ttattcatat ctcactggtc aacttattaa gtacacctat
    15061 ctcaaacatt ggttttaatg cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt
    15121 tgcccggtgg caacaattac ttttcaacac atcaatgaaa tccacattga aattattacc
    15181 aggtcaatgg caagacatta atttgaccct gaagggtgta tcgcccaata acctgggata
    15241 tctgaagtta gccatcaaca tggaaaatat tcagttcgac aatcttccct ctgctgaaaa
    15301 tcggcagaaa agaagcaaaa aataaatgca gttagtacgc ttataacaag gaatagcgag
    15361 catcattaat gaacaaaata tttaaagtta tctggaaccc tgcgacaggg aattatactg
    15421 ttaccagcga aacggcaaaa agccgtggca agaaatctgg gcgcagtaag ctgttaattt
    15481 ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc attggcgaat gccgggaatg
    15541 acaacggtca gggtgttgat tacggtagtg gatcagctgg cgacggctgg gttgctatag
    15601 gcaaaggggc gaaagcaaat acttttatga acaccagtgg ttccagtact gctgtgggtt
    15661 atgacgctat agctgaaggc caatatagct ctgccatcgg gtcaaaaacc catgcgattg
    15721 gtggtgcatc aatggccttt ggggttagtg caatatcaga aggcgataga agtatagcac
    15781 tgggtgcctc ttcgtattca ttgggccaat actcaatggc cctcggccgt tattcaaaag
    15841 cattgggtaa attgtctatt gctatggggg actcttccaa agcggaagga gcaaacgcca
    15901 ttgccctggg gcgtagcagt gtagctagcg gtactgacag cctcgcattt ggcagacaat
    15961 cacttgccag cgcagcgaac gctattgcga taggtgctga gaccgaagcc gctgaaaatg
    16021 caactgctat tggcaataat gcgaaggcaa aagggactaa tagcatggca atggggttcg
    16081 gaagccttgc cgataaagtc aatactatcg cattaggaaa tggcagccag gctctggcag
    16141 ataatgcaat cgccataggc cagggcaaca aagctgatgg cgtggatgcc atcgctctgg
    16201 gtaatggtag ccagtcgaga ggcttaaaca ccattgcctt aggcacagcc agtaatgcaa
    16261 ctggtgataa gagtcttgcg cttggtagta atagcagtgc caacggtatt aactctgtcg
    16321 cgctgggcgc agattccatt gcggatttag acaataccgt ctctgtcggc aatagttcat
    16381 taaaacgcaa gatcgttaat gtgaaaaatg gcgcgatcaa gtctgacagt tacgatgcca
    16441 ttaatggttc acagctttat gccattagcg actcggtagc aaaaaaggct tggaggaggg
    16501 gctgcagtag atgttgatga cggtactgtt acagcaccaa cctacaattt aaaaaatggt
    16561 agcaaaaata acgtaggggc tgcgctcgct gtacttgatg aaaacaccct gcaatgggac
    16621 caaaccaaag gcaaatacag cgctgctcat ggtactagta gcccaactgc cagcgtaatc
    16681 accgatgttg cggatggcac gatttcagcc tccagtaagg atgcggttaa cggttcccaa
    16741 ctgaaagcta ccaatgacga tgtcgaagcc aacaccacca atatcaccaa cctgacggat
    16801 tccgttggtg accttcaggc tgatgccctg ctctggaacg aaactaaaaa ggcattcagt
    16861 gcagctcacg gccaggatac caccagcaaa atcaccaacg ttaaagatgc cgacctgacg
    16921 gctgacagca ctgatgctgt taacggctct cagctgaaaa ccaccaacga tgctgtggcg
    16981 acgaatacca ccaatatcgc caataacact tccaatattg ccactaacac caccaacatc
    17041 tctaacctga ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac
    17101 aatggtgtat tcacggcagc tcatggcacc gagaccacca gcaaaataac caacgttaaa
    17161 gatggcgacc tgacgactgg cagcaccgat gccgttaacg gctctcagct gaaaaccacc
    17221 aacgatgccg tggcgacgaa taccaccaat atcgccacta acaccaccaa catctctaat
    17281 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
    17341 gtcttcactg cagctcatgg caacaatacc gccagcaaaa tcaccaatat cctggacggc
    17401 acagtcactg caaccagttc cgatgccatt aacggtagcc agctttatga cttaagcagc
    17461 aatatcgcca cctacttcgg cggcaatgct tctgtgaata ctgacggtgt gtttaccggt
    17521 ccaacctaca aaatcggtga aacaaattat tataacgtcg gcgatgcact ggctgcgatt
    17581 aactcctcat ttagcacgtc tctcggcgat gctctgcttt gggatgccac cgcaggtaaa
    17641 ttcagtgcca aacacggtac taatggtgac gcaagcgtga tcactgatgt cgcagatggt
    17701 gaaatttcag actccagttc tgacgcagta aacggctcac aactccacgg cgtgagcagt
    17761 tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg cagacggcac catcactgcg
    17821 ccgacgtaca ccattgctaa tgctgattac gataatgtcg gtgatgccct gaatgctatc
    17881 gataccactc ctgacgacgc tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt
    17941 agcgccgctc acggaaaaga taaaactgcc agtgtaatca ctaacgtcgc taacggtgca
    18001 atctctgctg ccagcagcga cgcgattaac ggctcacaac tctataccac caataagtac
    18061 atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg acggcaccat caccgcaccg
    18121 acttacacca ttgcgaacgc cgagtacaac aacgtcggtg acgccctgga tgcgcttgat
    18181 gataacgccc tgctgtggga taagactgcc aatggcggtg ctggagccta caatgccagc
    18241 catgacggta aagccagcat catcactaat gtcgctaatg gcagtattag tgaggacagt
    18301 accgatgcag tgaacggttc tcagttgaat gcgacgaata tgatgattga gcagaacacc
    18361 caaattatca atcagctcgc tggtaacacc gacgcaacct atatcgaaga aaatggcgct
    18421 ggtatcaact acgttcgtac taacgacaac gatttagcct ttaacgatgc aagcgcctct
    18481 ggtgtcggcg ctacagctgt aggttataac gctgtcgcgt ctggtgccag cagcgtagcc
    18541 attggtcaga acagcagcag caccgttgat accggtattg cgctgggtag cagctccgtt
    18601 tccagccgtg tgattgccaa aggttctcgt gacactagcg taacggaaaa tggcgtggtg
    18661 attggttatg acaccactga cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt
    18721 aaataccgcc aaatcatcaa cgtagctgat ggttcagaag cccatgacgc cgttacggtt
    18781 cgccagttgc agaacgctat tggagcggtc gccactacgc caaccaagta ctttcacgcc
    18841 aactcaacgg cagaagactc actggccgtt ggtgaagact cgctggcaat gggtgcgaaa
    18901 actgtcgtta atggtaatgc aggtattggt atcggtttga acactctggt tctggctgat
    18961 gcgatcaacg gcattgctat cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca
    19021 atgggtaacg gttctcagac cacccgtggt gcacagactg gctacaccgc ctacaacatg
    19081 gacgcaccgc agaactctgt aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag
    19141 atcacaaacg tcgcagctgg ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa
    19201 gtcactgatg agcgcgtagc gcaaaatacc cagagcatta ctaacctgaa caatcaggtc
    19261 actaatctgg atactcgcgt tactaatatc gaaaacggta ttggcgacat tgtcaccacc
    19321 ggtagcacca agtacttcaa gaccaacacc gatggcgtag atgccaacgc ccagggtaaa
    19381 gatagcgttg ctattggttc tggttccatt gctgccgctg acaacagcgt cgcactgggt
    19441 accggttccg ttgcagagga agaaaataca atctctgtag gttcttctac taaccaacgc
    19501 cgtatcacca acgtagctgc cagtgttaat gccaccgatg cggttaacgt gtcgcagctg
    19561 aaatcttctg aagcaggcgg agtgcgctac gacaccaaag ctgatggttc tatcgactat
    19621 agcaatatca ccctcggtgg tggcaacggc agtacgactc gtatcagcaa cgtctccgct
    19681 ggcgtcaaca acaacgacgc ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg
    19741 aagcaataca ccgatcagcg gatggttgag atggataaca aactgtctaa aactgaaagc
    19801 aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg gtctgccgca ggcttataca
    19861 ccgggtgcca gcatggcttc tattggtggc ggtacttaca acggtgaatc ggcagttgct
    19921 ttaggtgtat cgatggtgag cgccaatggt cgttgggtct acaaattaca aggtagtacc
    19981 aatagccagg gtgaatactc cgccgcactc ggtgccggta ttcagtggta atgatccatt
    20041 aagttagtgt gactaagggc aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag
    20101 ataaggcgct ccgggtcaac aatctaaggg caattctctg atgaggattg cccttttctt
    20161 taccagacat ctccccccac aagaattggc cctaccaatt cttcgcttat ctgacctctg
    20221 gttcacaatt ccccaattaa aactcacatc aatattgcca atacataaca tttagttaac
    20281 cattcattgt cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg
    20341 acaaggagat gagcaacaga ctcattacac gatgtgcgtg gactctagga gacctgcaat
    20401 gaatctctgg caacaaaact acgatcccgc cgggaatatc tggctttcca gtctgatagc
    20461 atcgcttccc atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt
    20521 cgccgcctca tggacggtag caatcgccct tgccgtggct ttgctgttct ataaaatgcc
    20581 ggtcgctaac gcgctggcct cggtggttta tggcttcttc tatggtttgt ggcccatcgc
    20641 gtggatcatt attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga
    20701 catcattcgc tcgtctattc tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt
    20761 cggtttctgt ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat
    20821 taccgccgca ttgctggtcg gcctgggttt taaaccgctg tacgccgccg gactgtgcct
    20881 gattgttaac accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg
    20941 acaagtaaca ggcatcgaca gctttgagat tggccagatg gtggggcgtc agctaccgtt
    21001 tatgaccatt atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa
    21061 agagacgtgg cctgcggtcg tggttgcagg cggctcgttt gccatcgctc agtaccttag
    21121 ctctaacttc attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg
    21181 cctgacgctg ttcctcaaac gctggcaacc agttcgcgta ttccgcttcg gtgacttggg
    21241 ggcgtcacag gttgatatga cgctggccca caccggttac accgcgggtc aggtgttacg
    21301 tgcctggaca ccgttcctgt tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt
    21361 taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc
    21421 gtacctcaat aaactggttg cccgtatgcc accagtggtc agcgaggcta cagcctatgc
    21481 cgccgtgttt aagtttgact ggttctctgc caccggcacc gccattctgt ttgctgcact
    21541 gctctcgatt gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac
    21601 gctgaaagaa ctggctctac ccatctactc catcggcatg gtgctggcgt tcgcctttat
    21661 ctcgaactat tccggactgt catcaacact ggcactggca ctggcgcaca ccggtcatgc
    21721 attcaccttc ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac
    21781 ctcatctaac gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc
    21841 tgatctgttg ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc
    21901 gcaatctatc gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt
    21961 ccgctttact gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga tcaccacgct
    22021 tcaggcttat gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca
    22081 gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg
    22141 aagttgcccg ctgagcgcca actggcgatg caactcggcg tatcacgtaa ttcactacgc
    22201 gaggcgctgg caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg
    22261 tttattcgct ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg
    22321 ctgatggccg atgatccgga ttacagtttc gatattctgg aagcccgcta cgccattgaa
    22381 gccagcaccg catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag
    22441 ctttgctttg aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt
    22501 tttcatctgg cgattgccga agcctcacat aacatcgtgc tgctgcaaac catgcgcggt
    22561 ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca
    22621 ccggtttttt cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgctggt
    22681 gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta gttttgttca caccaccatg
    22741 aaacgattcg atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat
    22801 aatgagcatt cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg
    22861 cagcgcaacg cattctgccg ccgttcctgt tccactatat ggatggtggt gcatattctg
    22921 aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc
    22981 tgaaaaacat gtccgactta agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc
    23041 cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg
    23101 cagccaaagc ggcggacgcg catggcattc cgtttactct ctcgacggtt tccgtttgcc
    23161 cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc
    23221 gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc
    23281 tggttttcac cgtggatatg ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta
    23341 tgagcggccc aaatgcggca atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg
    23401 cgtgggatgt gggcctgaac ggtcgtccac atgatttagg taatatctca gcttatctcg
    23461 gcaaaccgac cggactggaa gattacatcg gctggctggg gaataacttc gatccgtcca
    23521 tctcatggaa agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag
    23581 ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg
    23641 tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcgttgcccg
    23701 ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga attcgtaacg
    23761 ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt
    23821 tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg
    23881 aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcagt gaaattacgc
    23941 aagattcgct ggtgcagggg ctgggtaaag agttgcctac ggcactggct ccgatggcga
    24001 aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa ccctctccct cagggagagg
    24061 ggaccgttcg gcgctgtatg tactccctca ctctgaaacg acaccgcact ctttttttct
    24121 ccctcgcccc tccggggagg gccggggtga ggggaaaagg ccgcacggtt caccaatttt
    24181 ctgctatcct gcccccgcac taagggggca gtatgctaaa catcgtactt tacgaaccag
    24241 aaattccgcc aaatactggc aacatcatcc gtctttgcgc taataccggc tttcgtctac
    24301 atatcatcga accgatggga tttgcctggg acgacaagcg cctgcgccgc gcggggctgg
    24361 actatcacga gtttaccgcc gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag
    24421 aaaatcccca gcgcctgttt gccctcacca cgaaaggtac gcctgctcac agcgccgtaa
    24481 gctatcagga tggcgattat ctgatgttcg gcccggaaac acgcggcctg ccagcgagca
    24541 ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca
    24601 gcatgaatct gtccaatgcg gtgtcggtag tggtgtatga agcctggcgg cagctgggat
    24661 atccgggagc gttgttgaga gattagttac tgtatgccgg atagatgatt acatcgcatc
    24721 cggcacgatc acaggacatt agatcccatc cccatactca aatgtatggt taataccgtt
    24781 gaaatgctgg tccatatcca ttgatggctt atcgctgtct ggtttaccga caatacgagc
    24841 cggaacgcca gcggcggttg tatgcggcgg caccggttgc agcaccacgg aacctgcgcc
    24901 aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat
    24961 cacaccttca cgaattttcg ggtgacggtc accaccagat ttacccgtac cgccaagcgt
    25021 cacagattgc agaatcgata cgtcgttttc aattaccgcc gtttcaccca caacgatgcc
    25081 cgtggcgtgg tcgagcataa tgccacggcc aatttttgct gccgggtgaa tatcgacctg
    25141 gaacgtcaca gaaacctggt tttgcagaaa gattgccaat gcgcgacgcc cctgattcca
    25201 caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg
    25261 ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc
    25321 agaggcgatc atttccgggt cagcggcgta ggcttcttca accacttcac ggatagcaat
    25381 agcaggcata attggcgatg acagcttgtt cgccagcatg tagctcagtg cactgccaag
    25441 gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc agcattggct cacagtccgc
    25501 cagcgttctg gcttcggctt taatattgtt ccagacaatt tccagttctt cacacgacat
    25561 tgcttactcc acacgatgag ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg
    25621 taacaaaggt tccctggggt tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata
    25681 atgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa tacttgataa atttcctcgg
    25741 ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc gtattgcggt
    25801 agccttccac cacctgacca atcttctcct gcgcgctttg tacatccatg ccctgaccga
    25861 gcatcatacc aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc accagatcgc
    25921 ccagccccgc catgcccata aaggtggcag gatcggcacc cagcgccgca ccaagacgcg
    25981 acatttcagc cagcccacgg gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac
    26041 cgtcagacat ccccgcgcca atggcaatga cgtttttcac cgcgccgcca agctgcacgc
    26101 caatgaaatc cgggttgctg taaacgcgga aacttttgcc gcagtgcagc agctgctgga
    26161 gatcatcggc aaaggtctga tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg
    26221 ccagttcttt cgcaaacgtt gggccagaga taaccgccag cggaatttga tcgcctaagg
    26281 cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc ttccagccct ttggtcgccc
    26341 acaccagacg cgcatcagga cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga
    26401 catggctggg tacgacgacg agaatattac gactggctgc cagcgcagtg gcgagatcgc
    26461 tttcaagatg gagcgtatcg ggaaaaggca catcggggag aaacgcggcg ttacagcggt
    26521 cgcgttcaag cgttgcgata tgttcagggt catggcccca gaggacaacc tcgtggccat
    26581 ttcttgccag ggtaatggca agagcggtgc cgtacgagcc ggcaccgatc acagtcattg
    26641 aagcattacg ttggttcatc aggcatcctg atgttcttca gtaccttcgc cagcctgctg
    26701 ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa
    26761 tgtaccgcgg gataccatgc tggtgatgca ctcacgagca tacgggaaca gaatgttcgg
    26821 gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg atacccgcga tggagaaaat
    26881 accgccctgc tgaacttcac acaggaacgc ggtttcttcg cccaaggagg ccgttacggt
    26941 aacacgcagt accacttcgt atacgtcatc tgccagttgg gaagatgccg tatccagatc
    27001 aagtttaact tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa
    27061 agagatatcc ttggtataaa tacgttggat ctggaaagtc atttcagtgt tgttttgttc
    27121 tgacatgtgt agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt aataccaacg
    27181 ttcacacgtt atttcagcag gggatccagt ccaccacgtg catccaatgc atacaagtca
    27241 tcacagccgc caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg
    27301 cgtttgatca tctcttcacg cttggcggca ttgccatcga tcggcaactc ctggaaactc
    27361 acgcccttgc tgctcagcag tgcttttgca cgatggcaat acgggcaggt ttctttggta
    27421 tagatttcaa cattggccat aatttagctc ctgttttttt accctgaaat ttcatgttgc
    27481 agggcggctg caaaatggag tgtccccaga agcttataac aataagtcgc tgggggccta
    27541 attgcagcta acggcctgca tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga
    27601 ggcaagtttt cgccagccca gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa
    27661 ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc gtctaccacg
    27721 ataaccgatt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt gatttcgctc
    27781 ggcaacagat tgatagaacc tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa
    27841 tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg agtaatcacc
    27901 ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag taacgcgatc
    27961 caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg catggggggt
    28021 aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc
    28081 aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc
    28141 aaatctggca tcggcttgcc gcgagcggtc gtatattttg atcttcagag gctattttat
    28201 cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg
    28261 tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta tcgtgaagaa
    28321 cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact gtgggccaat
    28381 cgtccgcata ccctgatcga cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg
    28441 ggtaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg
    28501 actcgtctgg acgttgaaat caaagatcgc gctttctttg ctaatccagt gctggctggc
    28561 gcggtacata aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct gctctccgca
    28621 ggcggcgtac acagccacga agatcacatc atggcgatgg tagaactggc agctgaacgc
    28681 ggcgcagaaa aaatctacct gcacgcattc cttgacggtc gcgacacccc gccgcgcagt
    28741 gctgaatcct cgctgaaaaa atttgaagag aaatttgctg cgctgggtaa aggccgcgta
    28801 gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga tcgcgttgaa
    28861 aaagcctatg acctgctgac tctggcgcag ggtgagttcc aggccgatac tgccgttgct
    28921 ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt
    28981 cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg atgcgctgat tttcatgaac
    29041 ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg tgaacgctga tttcgatggc
    29101 ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac
    29161 atcaaaactg cggttgctta cccacccgct tccctggtta acaccttcgg cgagtggatg
    29221 gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact
    29281 ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac
    29341 tcaccgaaag tggctaccta cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa
    29401 aaactggttg cggccatcaa gagcggcaaa tacgacacca ttatctgtaa ctatccgaac
    29461 ggcgacatgg taggtcacac cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg
    29521 gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca actgctgatc
    29581 accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca ggcacacacg
    29641 gcacacacca acctgccagt tccgctgatt tacgttggtg ataagaacgt gaaagcggtt
    29701 gcaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg tatggaaatc
    29761 ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc catgagggga
    29821 aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat caggcccatc
    29881 atctacgcca gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat
    29941 gagcgtgacc aactcaaatc tattcaggcc gatatcgccg caaaagagcg cgcggtacgc
    30001 caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga agaagcgatc
    30061 tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact caataaacag
    30121 attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc
    30181 agcctcgccg cacaactgga tgccgcattc cgtcagggcg agcataccgg tattcagcta
    30241 attctcagcg gtgaagaaag ccagcgtgga cagcgtttac aggcttattt cggctatctc
    30301 aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg
    30361 cagcgtgctg aactggaaga gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc
    30421 gcccaacagg cgaaactgac tcaggctctg aacgagcgta aaaagacgct ggcggggctg
    30481 gaatcttcca tccagcaagg tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt
    30541 ctgcgtaaca gcattgcccg tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg
    30601 cgtgaggccc aggcggttcg cgagcgccag aaagaagcga cgcgcaaagg caccacctac
    30661 aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt
    30721 ggtcaggcat tctggccggt tcgcgggccg acgctgcatc gctatggcga gcagctacag
    30781 ggtgaattac gctggaaagg gatggttatc ggtgcctctg aaggtactga agttaaagca
    30841 attgccgacg gtcgagtgat tctggctgac tggctgcaag gttacggtct ggtggtggtg
    30901 gttgagcacg gtaaaggcga catgagtctt tacggctata atcagagcgc actggtgagc
    30961 gttggttcgc aggttcgcgc gggccagcca attgcactgg tgggcagcag tggcggtcag
    31021 ggtcggcctt cactctattt cgaaattcgc cgccagggtc aggcggtcaa tccacagccg
    31081 tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg
    31141 ttggcgctct cctcccccgt acttgctggc aaacttgcca tcgtcattga tgattttggg
    31201 tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta
    31261 ttacccgatt caccgcacgc cagagagatg gcgaccaaag cgcataacag cggtcacgaa
    31321 gtgttgattc atctcccgat ggcaccgttg agtaaacagc cgctggagaa aaatacgcta
    31381 cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt caataacgtg
    31441 ccctatgccg tggggatcaa caaccacatg ggcagcaaga tgacctctaa cctgtttggt
    31501 atgcagaaag tgatgcaggc gctggagcgt tacaatcttt acttcctcga cagcgtaacc
    31561 atcggtaata cccaggcgat gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg
    31621 aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca
    31681 attgatctgg cgcgtcgcaa cggttcaacc attgccattg ggcatcctca cccttcaacg
    31741 gtacgcgtgc tacaacagat ggtttataac ctgccgccag acattacgct ggtgaaagcc
    31801 agcagcttgc tgaatgaacc gcaggttgat acttctacac cgccgaaaaa cgctgtgcct
    31861 gatgcaccgc gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa
    31921 cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg
    31981 atcgtttact tccagcatca gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt
    32041 aacgctagcg caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcac
    32101 gccattgcca taagcggaaa gaccacagta acgcctggta accgaccttc acgctgcgca
    32161 cattggtaat caggcgctta tacatacccg gaagtaaata tctcggcaat catccgttgg
    32221 cgggtaagaa tatccggctc tttgcgca
//
LOCUS       DAJTOW010000038        31189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTOW010000038 DAJTOW010000000
VERSION     DAJTOW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.038"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            393..2051
                     /locus_tag="OO244_001882"
     CDS             393..2051
                     /locus_tag="OO244_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5663726.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="OO244_001883"
     CDS             complement(2048..3004)
                     /locus_tag="OO244_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5663727.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="OO244_001884"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="OO244_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5663728.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="OO244_001885"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="OO244_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5663729.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO244_001886"
     CDS             complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO244_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5663730.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6833..7153
                     /gene="clpS"
                     /locus_tag="OO244_001887"
     CDS             6833..7153
                     /gene="clpS"
                     /locus_tag="OO244_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5663731.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7184..9460
                     /gene="clpA"
                     /locus_tag="OO244_001888"
     CDS             7184..9460
                     /gene="clpA"
                     /locus_tag="OO244_001888"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5663732.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9863..9950)
                     /locus_tag="OO244_001889"
     tRNA            complement(9863..9950)
                     /locus_tag="OO244_001889"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9914..9916),aa:Ser,seq:gga)
     gene            complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO244_001890"
     CDS             complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO244_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5663733.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO244_001891"
     CDS             complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO244_001891"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5663734.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO244_001892"
     CDS             complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO244_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5663735.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO244_001893"
     CDS             complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO244_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5663736.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO244_001894"
     CDS             complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO244_001894"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5663737.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="OO244_001895"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="OO244_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5663738.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21230
                     /gene="ftsK"
                     /locus_tag="OO244_001896"
     CDS             17202..21230
                     /gene="ftsK"
                     /locus_tag="OO244_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5663739.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21385..21996
                     /gene="lolA"
                     /locus_tag="OO244_001897"
     CDS             21385..21996
                     /gene="lolA"
                     /locus_tag="OO244_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5663740.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22007..23350
                     /gene="rarA"
                     /locus_tag="OO244_001898"
                     /gene_synonym="mgsA"
     CDS             22007..23350
                     /gene="rarA"
                     /locus_tag="OO244_001898"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5663741.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23441..24733
                     /gene="serS"
                     /locus_tag="OO244_001899"
     CDS             23441..24733
                     /gene="serS"
                     /locus_tag="OO244_001899"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5663742.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24973..27416
                     /gene="dmsA"
                     /locus_tag="OO244_001900"
                     /pseudo
     CDS             24973..27416
                     /gene="dmsA"
                     /locus_tag="OO244_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27427..28044
                     /gene="dmsB"
                     /locus_tag="OO244_001901"
     CDS             27427..28044
                     /gene="dmsB"
                     /locus_tag="OO244_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5663743.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28046..28837
                     /locus_tag="OO244_001902"
     CDS             28046..28837
                     /locus_tag="OO244_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5663744.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28873..29499)
                     /locus_tag="OO244_001903"
     CDS             complement(28873..29499)
                     /locus_tag="OO244_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5663745.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29814..30962
                     /locus_tag="OO244_001904"
     CDS             29814..30962
                     /locus_tag="OO244_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5663746.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7691 a         7647 c         8374 g         7477 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ttaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc
     6241 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
     6301 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
     6361 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
     6421 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
     6481 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
     6541 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
     6601 atacaattta gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta
     6661 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
     6721 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
     6781 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
     6841 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
     6901 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
     6961 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
     7021 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
     7081 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
     7141 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
     7201 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
     7261 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
     7321 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
     7381 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
     7441 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc
     7501 aaacgttctg gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
     7561 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
     7621 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
     7681 ggaacgtatg gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga
     7741 cccactaatt ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg
     7801 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg
     7861 tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta
     7921 ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg
     7981 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
     8041 gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct
     8101 aatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
     8161 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
     8221 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
     8281 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt
     8341 gaaatacatt aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg
     8401 cgctcgcgca cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
     8461 tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg
     8521 cgataccctg aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc
     8581 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
     8641 accggtcggt tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt
     8701 acagctttcg aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga
     8761 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg
     8821 cggtttgctg accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat
     8881 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
     8941 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa
     9001 cgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac
     9061 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
     9121 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
     9181 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
     9241 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
     9301 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
     9361 ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt
     9421 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg
     9481 gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa
     9541 aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga
     9601 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9661 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9721 tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac
     9781 ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9841 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
     9901 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
     9961 gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
    10021 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat
    10081 ctacatcgag acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata
    10141 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
    10201 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
    10261 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
    10321 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    10381 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    10441 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    10501 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    10561 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    10621 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    10681 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc
    10741 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    10801 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    10861 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    10921 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    10981 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg
    11041 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    11101 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    11161 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    11221 cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca
    11281 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    11341 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    11401 accaagcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    11461 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct
    11521 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    11581 gatgggtgac cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct
    11641 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    11701 catgtaacag cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    11761 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc
    11821 gacgcaagat ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    11881 cacgcagcgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    11941 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    12001 ggtcccatgc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    12061 gaatcgctat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    12121 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    12181 gagtttgggt atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta
    12241 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    12301 caaacgcggc taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc
    12361 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca
    12421 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    12481 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    12541 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca
    12601 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    12661 gtaacataac gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    12721 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat
    12781 gatcgagcgt atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg
    12841 ccagtccggc aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca
    12901 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    12961 tgattgcggc accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    13021 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    13081 tggcgagcag cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt
    13141 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    13201 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    13261 tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg
    13321 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    13381 cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta
    13441 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    13501 cgccaacagg tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    13561 tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat
    13621 cccgcaattt tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc
    13681 atgattctgg tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg
    13741 agagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca
    13801 acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    13861 ccggcgacgt gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt
    13921 ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    13981 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga
    14041 aaaactctgg cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt
    14101 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    14161 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt
    14221 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    14281 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    14341 agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg
    14401 ccataaacaa cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag
    14461 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    14521 gatagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc
    14581 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    14641 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    14701 agaccaccca tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg
    14761 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    14821 gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt
    14881 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca
    14941 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa
    15001 aaataaaggc gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta
    15061 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    15121 gccagtaccg gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc
    15181 aaagacgcca ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat
    15241 gtagccgttt tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat
    15301 agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    15361 cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt
    15421 gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    15481 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    15541 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    15601 gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga
    15661 gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta
    15721 ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg
    15781 atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa
    15841 cggaccggtc agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag
    15901 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta
    15961 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    16021 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttac
    16081 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    16141 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    16201 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    16261 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccacagca gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc
    19621 cgcaacaacc ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac
    19681 agccgcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
    19741 acaaaccgac gacgccgctg ccttctctgg atttgctgac accgccgccg agcgaagtgg
    19801 agccggtaga tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg
    19861 atttccgtat taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg
    19921 aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg
    19981 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    20041 taggtctgga gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata
    20101 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    20161 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    20221 gttccggtaa atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc
    20281 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    20341 gcattccgca cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc
    20401 gctggtgtgt taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta
    20461 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    20521 acccgtactg gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac
    20581 catacattgt cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    20641 tggaagagct gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac
    20701 tggcaactca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    20761 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    20821 gcgcggaatc actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc
    20881 cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact
    20941 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag
    21001 gtggtgcggg tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc
    21061 agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg
    21121 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    21181 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt
    21241 gccggataag gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt
    21301 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    21361 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca
    21421 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat
    21481 aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    21541 gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg
    21601 acacaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    21661 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    21721 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    21781 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    21841 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    21901 tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    21961 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    22021 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    22081 agcacagtat atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    22141 cgaagccggg catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    22201 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac
    22261 ctctggcgtg aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg
    22321 tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc
    22381 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    22441 gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct
    22501 gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta
    22561 tggtggtcag gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa
    22621 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta
    22681 tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag
    22741 cgcccgcttt gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc
    22801 ggtacgtggt agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg
    22861 tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa
    22921 tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    22981 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa
    23041 aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga
    23101 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    23161 cgggcaggaa tatcgttacg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    23221 cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    23281 caagattggt gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    23341 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    23401 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    23461 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    23521 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    23581 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    23641 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    23701 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc
    23761 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg
    23821 taccccgcgt gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc
    23881 tggcctcgac tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg
    23941 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    24001 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    24061 gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc
    24121 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg
    24181 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    24241 ccgttctgaa gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    24301 cgacaaagtt gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga
    24361 gatgactggt catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat
    24421 cctttgcact ggcgacatgg gctttggcgc ctgcaaaact tacgacctgg aagtatggat
    24481 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    24541 acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    24601 cctgaacggt tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    24661 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    24721 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt
    24781 gtctcccttt gataccgaac aataattact cctcacttac acgtaatact actttcgagt
    24841 gaaaatctac ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga
    24901 gctgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt
    24961 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc
    25021 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
    25081 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
    25141 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
    25201 gtcgtggacg gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg
    25261 gcctacacta ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc
    25321 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    25381 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    25441 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    25501 cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt
    25561 atcttaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct
    25621 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc
    25681 tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    25741 aacaggcacg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    25801 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg
    25861 ttaacggtct ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata
    25921 aatattgcgt tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt
    25981 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    26041 cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    26101 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    26161 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga
    26221 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    26281 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    26341 aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    26401 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    26461 atgaaatcct ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    26521 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    26581 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    26641 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    26701 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    26761 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    26821 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    26881 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    26941 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    27001 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    27061 ttacaggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    27121 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc
    27181 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    27241 tgacgccacg aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg
    27301 atgcaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    27361 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    27421 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    27481 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    27541 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    27601 tttgcctact atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc
    27661 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    27721 tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    27781 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    27841 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    27901 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    27961 ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat
    28021 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    28081 cttcacggtc ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct
    28141 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    28201 ggtactgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    28261 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    28321 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    28381 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    28441 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    28501 gatgggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg
    28561 cgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc
    28621 cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt
    28681 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    28741 atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    28801 tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc
    28861 cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata
    28921 atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg
    28981 gtgcagctcg caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg
    29041 atcccacgcc gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa
    29101 gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac
    29161 cacaccggca ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc
    29221 gttatcccag gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc
    29281 tttcagttct agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt
    29341 tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc
    29401 gggttcgata tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc
    29461 agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc
    29521 agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat
    29581 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    29641 atcaataaaa acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt
    29701 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    29761 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    29821 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg
    29881 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg
    29941 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    30001 atgtcattaa acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg
    30061 ctggctgtgc cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg
    30121 tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg
    30181 ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa
    30241 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt
    30301 gggttacagg tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc
    30361 agcaggctga aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc
    30421 ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga
    30481 tgcctctgta cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg
    30541 tactggtcag tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg
    30601 gtcgactgct ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc
    30661 ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat
    30721 atccggtggc gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga
    30781 accaagcctt actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta
    30841 tgctaatgca gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta
    30901 tctatttgct gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt
    30961 aaatgaattc aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta
    31021 tctgacttgc ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa
    31081 ttttgcattt cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga
    31141 tgccccttta attttggcga aggatttgtc tatggctggg aatgttcag
//
LOCUS       DAJTOW010000039        30452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTOW010000039 DAJTOW010000000
VERSION     DAJTOW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.039"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..369)
                     /locus_tag="OO244_001905"
                     /pseudo
     CDS             complement(1..369)
                     /locus_tag="OO244_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(344..1144)
                     /gene="otsB"
                     /locus_tag="OO244_001906"
     CDS             complement(344..1144)
                     /gene="otsB"
                     /locus_tag="OO244_001906"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5663747.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1311..2297)
                     /gene="araH"
                     /locus_tag="OO244_001907"
     CDS             complement(1311..2297)
                     /gene="araH"
                     /locus_tag="OO244_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5663748.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2312..3826)
                     /gene="araG"
                     /locus_tag="OO244_001908"
     CDS             complement(2312..3826)
                     /gene="araG"
                     /locus_tag="OO244_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5663749.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3896..4885)
                     /gene="araF"
                     /locus_tag="OO244_001909"
                     /pseudo
     CDS             complement(3896..4885)
                     /gene="araF"
                     /locus_tag="OO244_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5682..6185
                     /locus_tag="OO244_001910"
     CDS             5682..6185
                     /locus_tag="OO244_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5663750.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6265..6516)
                     /locus_tag="OO244_001911"
     CDS             complement(6265..6516)
                     /locus_tag="OO244_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5663751.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6631..6717)
                     /gene="azuC"
                     /locus_tag="OO244_001912"
     CDS             complement(6631..6717)
                     /gene="azuC"
                     /locus_tag="OO244_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5663752.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            6980..7303
                     /locus_tag="OO244_001913"
     CDS             6980..7303
                     /locus_tag="OO244_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5663753.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7474..7971
                     /gene="ftnA"
                     /locus_tag="OO244_001914"
     CDS             7474..7971
                     /gene="ftnA"
                     /locus_tag="OO244_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5663754.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8008..8247)
                     /locus_tag="OO244_001915"
     CDS             complement(8008..8247)
                     /locus_tag="OO244_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5663755.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8437..9648
                     /gene="tyrP"
                     /locus_tag="OO244_001916"
     CDS             8437..9648
                     /gene="tyrP"
                     /locus_tag="OO244_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5663756.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMMASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9710..10375)
                     /gene="yecA"
                     /locus_tag="OO244_001917"
     CDS             complement(9710..10375)
                     /gene="yecA"
                     /locus_tag="OO244_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5663757.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10571..10657)
                     /locus_tag="OO244_001918"
     tRNA            complement(10571..10657)
                     /locus_tag="OO244_001918"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10621..10623),aa:Leu,seq:taa)
     gene            complement(10670..10743)
                     /locus_tag="OO244_001919"
     tRNA            complement(10670..10743)
                     /locus_tag="OO244_001919"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10709..10711),aa:Cys,seq:gca)
     gene            complement(10798..10873)
                     /locus_tag="OO244_001920"
     tRNA            complement(10798..10873)
                     /locus_tag="OO244_001920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10838..10840),aa:Gly,seq:gcc)
     gene            complement(11025..11573)
                     /gene="pgsA"
                     /locus_tag="OO244_001921"
     CDS             complement(11025..11573)
                     /gene="pgsA"
                     /locus_tag="OO244_001921"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5663758.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11630..13462)
                     /gene="uvrC"
                     /locus_tag="OO244_001922"
     CDS             complement(11630..13462)
                     /gene="uvrC"
                     /locus_tag="OO244_001922"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5663759.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13459..14115)
                     /gene="uvrY"
                     /locus_tag="OO244_001923"
     CDS             complement(13459..14115)
                     /gene="uvrY"
                     /locus_tag="OO244_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5663760.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14411..14587
                     /locus_tag="OO244_001924"
     CDS             14411..14587
                     /locus_tag="OO244_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663761.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14574..14798
                     /locus_tag="OO244_001925"
     CDS             14574..14798
                     /locus_tag="OO244_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5663762.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14831..15451)
                     /gene="sdiA"
                     /locus_tag="OO244_001926"
                     /pseudo
     CDS             complement(14831..15451)
                     /gene="sdiA"
                     /locus_tag="OO244_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15681..16433)
                     /gene="yecC"
                     /locus_tag="OO244_001927"
     CDS             complement(15681..16433)
                     /gene="yecC"
                     /locus_tag="OO244_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5663763.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16430..17098)
                     /gene="tcyL"
                     /locus_tag="OO244_001928"
     CDS             complement(16430..17098)
                     /gene="tcyL"
                     /locus_tag="OO244_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5663764.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17113..18099)
                     /gene="dcyD"
                     /locus_tag="OO244_001929"
     CDS             complement(17113..18099)
                     /gene="dcyD"
                     /locus_tag="OO244_001929"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5663765.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18204..19004)
                     /gene="tcyJ"
                     /locus_tag="OO244_001930"
     CDS             complement(18204..19004)
                     /gene="tcyJ"
                     /locus_tag="OO244_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5663766.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19092..19643)
                     /gene="fliZ"
                     /locus_tag="OO244_001931"
     CDS             complement(19092..19643)
                     /gene="fliZ"
                     /locus_tag="OO244_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5663767.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19689..20408)
                     /gene="fliA"
                     /locus_tag="OO244_001932"
     CDS             complement(19689..20408)
                     /gene="fliA"
                     /locus_tag="OO244_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5663768.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(20573..22225)
                     /gene="fliC"
                     /locus_tag="OO244_001933"
     CDS             complement(20573..22225)
                     /gene="fliC"
                     /locus_tag="OO244_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5663769.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22491..23897
                     /gene="fliD"
                     /locus_tag="OO244_001934"
     CDS             22491..23897
                     /gene="fliD"
                     /locus_tag="OO244_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5663770.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23922..24332
                     /gene="fliS"
                     /locus_tag="OO244_001935"
     CDS             23922..24332
                     /gene="fliS"
                     /locus_tag="OO244_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5663771.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24332..24697
                     /gene="fliT"
                     /locus_tag="OO244_001936"
     CDS             24332..24697
                     /gene="fliT"
                     /locus_tag="OO244_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5663772.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24775..26262
                     /gene="amyA"
                     /locus_tag="OO244_001937"
     CDS             24775..26262
                     /gene="amyA"
                     /locus_tag="OO244_001937"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5663773.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26296..26709)
                     /locus_tag="OO244_001938"
     CDS             complement(26296..26709)
                     /locus_tag="OO244_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5663774.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26896..28101
                     /gene="yedE"
                     /locus_tag="OO244_001939"
     CDS             26896..28101
                     /gene="yedE"
                     /locus_tag="OO244_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5663775.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28098..28331
                     /gene="yedF"
                     /locus_tag="OO244_001940"
     CDS             28098..28331
                     /gene="yedF"
                     /locus_tag="OO244_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5663776.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28438..29108
                     /locus_tag="OO244_001941"
                     /pseudo
     CDS             28438..29108
                     /locus_tag="OO244_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29148..30356)
                     /locus_tag="OO244_001942"
                     /pseudo
     CDS             complement(29148..30356)
                     /locus_tag="OO244_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7542 a         7864 c         7256 g         7790 t
ORIGIN      
        1 taatgtcatc gtcttgcaac agcggcagta atttatctgc cagcaacgca tttacgcgta
       61 gatagccgtc ccaggcagga cgctgaaatt gcaccagatc gagccgataa tgaaaagcgg
      121 gccagagaac ggcattggag aattggttgt agtattcgtc aaggtcctgt tcgctgaggt
      181 taaaagaggc ccacgtaatg ttaccttttt tagcggctga tcctcattcc ctgtttcacc
      241 actccagcca aaccacagtc cgcctgcggc tttcagtgcc cccagtatgc caacggcaag
      301 gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc cggttagata ctacgactaa
      361 acgactcata gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg gcggttatca
      421 tttcaagcca gctccagaca tccggcacac ccgccagtcg ccatgatgcc tgagttgcac
      481 ctgtgccaat ttttactgac attccgccca gtcggttaac gactgcgaag ccagattcat
      541 cggttaaatc atcgcccaga aatacgggcg ttcgcccgat aaaaggagct tcctgcataa
      601 aggctgcaat cgcttcacct ttactggtac ctctcggttt gatctcgaca acacactttc
      661 cctgctgtaa cgccatttgt ggccagatct gagtaatacg ttgcgctaat gtcattaatg
      721 cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa agccatccct ttcgcctcca
      781 gctccgcgcc gggatactga gcgatgaccg tatgcagttg cacgctaata tcacgcgcaa
      841 tcgcatccgg cagatgaacg atatgtgttt taccattgat gtcacggcgc tccgccccat
      901 gcacgcccgc taacgggaag cgataaggtt ttgccagtgc gtcaagctcc accattgagc
      961 gccctgatat caatgccaat gcaccatcac ttgcggttgc cagtagctgt agtccttgca
     1021 gaatattgtc aggcacgacg acctgatcgg gatgcggttt gatttccgcc agcgttccat
     1081 caagatcaaa aaaccaggca tatttcgcgg atagttcagg ggtttcggtt aacggttctg
     1141 tcacccggct ctcctccttc tcttttctta ttgtgttgcg cagaccgcgc tttacagaca
     1201 tgtaagtata gacagtgtga cggaggtcgc catttgcaac gaagaacagc caaccgttgt
     1261 ggcggctggc tgtggtggga aaaaacgtta agttgttgtg gaaaaaagca tcagacagtg
     1321 cgcttcgctt tttgcttgta acggtcgaag atcaccgctg ccagcaggat taagccgcga
     1381 accacgtact gcgcgaaagg agaaatatta agcaggttca tggcgttttc cacggtgcct
     1441 aaaattaaga tacccgccac cacatatgag atttttccga tgccaccttt cagagaaacg
     1501 ccacctaaaa cgcaggcgga gataacaata agctcataac caatcgacgt cattggctgc
     1561 ccactagtca tacgtgaagc cagaataatt ccggctatcg cagataccag gccagagaga
     1621 acaaagataa taattttggt gcgaacaacc ggtacacccg ccagacgcgc agcctcttcg
     1681 ttcccgccaa tcgccagggt attacgacca aaggtggttt tattcagcag caaaccaaag
     1741 ataatcagac acgcgacggt gagccagatt ggcgcaggca gaccgaacca gttagcgtaa
     1801 ccaagggcaa agaagctttc atcttcgata ccgaccgctt taccgtctga aatgatgtac
     1861 gccagacctc gaacaatctg catcgttgcc agtgtcgtga tcagagcatt tattttcagt
     1921 ttggcgataa caaagccatt gaccaggcca cagagaacgc ccagcaacaa acccgctgcc
     1981 acgccaatcc acaggctttc agtcaggttg ataaccaccg ccgtggtgac acccgcacag
     2041 gcaattacgg aggcgacaga aaggtcaaag tcaccggaag caaggcagaa caacatgcca
     2101 cacgccacca tccccgacat ggaaattgcc aggcccaacc ctttcatatt aatgaaggtg
     2161 gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca ccgcaaacac caccagcatg
     2221 ccgtactgat cccagatacg cccgaagctg aatgacgact taggtgcgcc agaccccgat
     2281 gtagaaacag aagacatcat actctcctta ctcaggcaac tgcctggctg actttaggca
     2341 tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg taacaattca ccggcgattt
     2401 cgccttcccg catcaccaca atccggtcgg caacgccgag gacttcaggt aagtcgctgg
     2461 aggcaaacag caccgccacg ccctgcgccg ccagcgcata aattacgtta tatatttcgt
     2521 gcttagcgcc aacatcaatg ccgcgcgtag gttcatccag caaaatgacc ttcatctctt
     2581 ccgataacca gcggcccaga atggcttttt gctgatttcc gcctgagaga ttcatgatca
     2641 gttgctccgc gcccggcgtt ttgatgttga gcgaacgaat gtgctgatcg gcattgtttt
     2701 cttcccaact gttgttgatt acacaaccgc cgagcacatg tttgcgtctg gcactgatgt
     2761 tgatattgtc gcgaacggag tgcacgggaa taatgccttc cgctttgcga gcttccgggc
     2821 agagcatcat gcctgcggca atggcgtggc tcggtttacg aatatcgatc ggctgttggt
     2881 cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa caagcctttc attaattcgc
     2941 tacgccccgc ccctaccaga ccaaacagcc caacaatttc accactgcga accgccagac
     3001 ttattggcgt acgcacgcct agtgctttca cagcatcaag acgcaggcgc tcctcgccat
     3061 aactacgagg ttgccagccg tagatatcgc caatgtcgcg cccgaccatc gcctgcacca
     3121 gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt gacataacgt ccatctttaa
     3181 agacagtgat ggcatcgctg agggcaaata tttcttccat acggtgagaa acgtataaga
     3241 tcacccgccc ctcttttcgc agttcacgaa taacgcggaa aagattgccg atttcgcggg
     3301 cagaaaggga gctggttggc tcatcaaagg cgataatttt agcgttacgc gccagcgctt
     3361 tggcgatttc aaccatctgc cactgaccaa tggcgagata tttcagcggc gtgtccgggt
     3421 caatatccat accaagatgt ttaagttgta aacccgcctc ataattcagc aatgagcgat
     3481 tcacaatgcc gcctttatgc ggcagctggc cgagatagat gttttccgcg acggtcattt
     3541 ccggcacgag atgcagttcc tggtaaataa tcgccacacc cgcgttaagt gctgcggtcg
     3601 tgtcggaaaa ggacatttcc tgcccattaa tcactacaga acccgtggtt ggcgcatagt
     3661 tgccgctgag gatttttaag agagttgatt ttcctgcgcc attttcaccc atcaacgcat
     3721 gaacctgacc ggcatagcag tcaaaactaa tatccgtcag cgccttaacg ccgggaaacg
     3781 ttttaccgat gccgcgaaat gagagatacg gggtagactg ttgcataacg tctccgtgaa
     3841 tcactggtcg ttcacacccc ctctgcatca tgcagagggg aatttttccg gcaaattact
     3901 taccgcctaa accttttttc tccagttctt ctttaaagtt gtcacgcgtg atcagtacca
     3961 cgtctgtaac ttcggtaaat tttggcgttt caacgtcttt tgctacccag ttgtaaagca
     4021 tttcgctgga tttatagcca tgtacgtccg ggcttggcag cagggaaccg tagaagccgg
     4081 ttgcctgtgc tttagacagt tcgcttactg catccacacc gttaatacca ataccgatga
     4141 tatcggctgc tttaaagccc tgaccttcgg ttgcacgtac gccgccaagt acggtgctgt
     4201 cgttcatacc gacgatcagc caatgtttaa cttccggatg ttgaaccagc attgagttgg
     4261 cagcgtcaaa tgcccccggg atgtcgttag atttggtagg tacctgataa atttgttttt
     4321 ccgggaatcc tgcagctttc agtgcgtcca tagaacccgt tgtacggcgg cgggcggtat
     4381 ccagttcgtt ggcggtaatc gccatcactg cgctttcttt gacatcccag ccacgtttct
     4441 gcatctcttt atacagttcc tagccctgac gttcgccaat tttagtcgcc gccatcatca
     4501 ccagaggaac ggtatccatt ggcttacctt tggcgttaac aaactggtca tccactgcaa
     4561 tgactttcat atcgtagcca cgcgctttcg cgacgatggc agagccgagt ttcgggtccg
     4621 gagtacaaat aacgaaacct tttgcgccac tggctgccag gctgtcgatc gcgttcaatg
     4681 ttttttcgcc atccggcacg gcaatcttaa taacctcaaa ccctaaatcc ttcccggctt
     4741 tatcggcaaa cttccattcg gtctggaacc acggctcttc cggttgcttc accagaaaac
     4801 cgagcttcag gttctccgcc atagcggatt gtgacataac ggctgccaga ccaatggctg
     4861 ccagggcttt agtaaatttg tgcatggttc tctccagctt tagtgtcgtt ttgtgtaggg
     4921 caaaaacgaa tgacattcgt taaattaatc ggaaaacaaa gcattacctt ttatctaaaa
     4981 gataagtgac cgtgttgaca tagttttagc gagaaattaa ttctccatag gagagcaata
     5041 tcacatcgca gaattacagt gagaacgtgc ataaatttag cgggaaaaga cataaggaaa
     5101 agccaatttg tcagacaaat tgttgaatgc acagcagatt aatccataag attagcctgg
     5161 aaatccttgt tgtcttcggt acccatgcgg gatgtcttct ttttaaccag tcaataggcc
     5221 gcattacctg gcgttgagtt tttgaaatgg tgtaataacc acaactcaaa gatgtggaaa
     5281 atgcgcgtca ttcatttcgc cattaattat cactgtgctc attaattaac agaacacgta
     5341 taatgagagc catctcgcaa aaatgaaaaa acgttttata aaatcatcac ttcatcatga
     5401 attcaaattc atcgattaat atcaacaaga tacaaaaagc actatcatta aaattcattg
     5461 catttacatt gatttcacca atgaaatgta aaaatatata aacttgatga tttaagcatt
     5521 ttcttataac cgttcagacg ttattcttat ttcagatcat cgtcagaatt gactccacga
     5581 tcacatttcg gaccggcaga aaggaattat tctgcaaaca gtaattatgg tgttttgatt
     5641 tatcttgcac ctctccactt ctggatataa ggatattagg tatggcaacc gctggaatgc
     5701 ttctcaaact caactctcaa atgaaccgcg agttttacgc atccaatctc taccttcacc
     5761 tgagtaactg gtgttctgaa cagagtctga acggcaccgc cactttcctt cgcgcccagg
     5821 cacagagtaa tgtgacccaa atgatgcgca tgtttaactt tatgaagagt gtcggcgcta
     5881 cccccatcgt taaagccatt gatgttccag gtgaaaaact gaactctctg gaagaactgt
     5941 tccaaaaaac gatggaagaa tacgagcaac gttcaagcac tctggcgcag ttagccgatg
     6001 aagcgaaaga actgaatgat gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac
     6061 agcagcatga tggcctgttg ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg
     6121 cgggtatgtg ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca caccagctgc
     6181 attgatcata catcggcgct aatacattgc gccgatgaag tttttgagaa accgctgcct
     6241 catctgtttg aagcagcggt ttttttaatg ggattcaccc tgtggggtaa acttgagttc
     6301 aatcagcgcg atggcttttt ggattgcccg catggtgacc gggtctgcgg cagcgggatg
     6361 atgagtaaag tcgatattct tcagctgact ggacattttt tcacgaactt caacgggcgc
     6421 gattacatcg agaacatcca gaatttgttt gataaccaac tggcaagcta ccacatcaga
     6481 aaccagttcc tgatcggcat tcagcggctg ggacatcgta aactcctgat agcattttga
     6541 aagccgttat agtagcggct tcacatcttc agcgatagtc acatccaccg tcatcaggac
     6601 acaaaaaaac ctgccggagc aggttttttg ttatcggaac atattgcctg gcggtacgtc
     6661 tttgaacgtc ttgcaatagt tattgaacat acttttcagg attttgcgca gtttcatcgc
     6721 ggcactccga ccatttgtta tacaggtgtt attgtctttg cgtttataat atgacaacca
     6781 tcacaaaaat caatctttat gtgatacaaa tcacataaat acccctttaa tgttataaaa
     6841 atgataatca aaaaacagcc cccctatttc tgacacctac agatggcaag aaatagcgcc
     6901 tgccaggcgt cttttccggc cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc
     6961 ttactcttct ggctggacca tgagacttct gattctgact ctttcattaa taacgctggc
     7021 ggggtgtacg gttactcgcc aggcccatgt gagcgaagtt gacgcggcaa caggtattgt
     7081 acggttggtt tatgaccagg cttttttgca gcatgcccat accgatcgtt atgtcagtcg
     7141 cgggatcgcc gatcgcgcat gccagcagga aggctatacc cacgcgatcc cctttggtca
     7201 gccagtaggc aactgtagcc tttttgcggg ttccttatgt ttgaatacgg aattcacttt
     7261 gtcatatcag tgccatcatt ccgccttccc tgtctttctc taaatatttt aatcagcgag
     7321 gggatcttcg ctgattaaag aaatagcggt aatgcgtttt attcccattc gtatttttaa
     7381 taattgaagt ttatatttta ccttttgcaa ataataaaat aacaaattat agtgacgcca
     7441 cagcaacaaa tataaccttt gtggagcact atcatgctga aaccagaaat gattgaaaaa
     7501 cttaatgagc agatgaacct ggaactgtac tcttcactgc tttatcagca aatgagcgcc
     7561 tggtgcagct atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa
     7621 gagatgacgc atatgcagcg tctgtttgat tacctgactg ataccggcaa tttaccgcgt
     7681 attaataccg ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt attccaggaa
     7741 acctataaac acgaacaatt aatcacccag aaaattaacg aactggctca tgctgcaatg
     7801 accaatcagg actacccaac atttaatttc ctgcagtggt atgtttctga gcagcatgaa
     7861 gaagagaaac tgttcaaatc gattattgat aaattaagcc tggcaggcaa aagcggcgaa
     7921 ggtctgtatt ttatcgacaa agaactctct accctcgaca cacaaaacta atgctctccg
     7981 gcggcaggtt tctgccgccg tttcaaatca gtgacggcaa atcttactct gatcggtcga
     8041 aaaaccttct tctgaaggta taaatttgcc ttttgctgcc agaaacgcta ccagctctcc
     8101 cgctgtcacc ccttctgccg agcacgtgtg aaaacgtgcc tgttcaccaa aacgcgcttt
     8161 aatcgcagct tccagactgg catgcgtata ttgctcgcct gattcaatca tcatatttaa
     8221 cacttcatga ccgtgaatag agtccatcgt tcctcctcaa aaaaagccta gcgtagcgat
     8281 taccgcttat gaagactttg cgccagcgca ggactgaatg cttttattgt acatttatat
     8341 ttacacaata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag
     8401 atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt
     8461 gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg
     8521 gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc gttgatgtgc
     8581 tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac cggtctgggc
     8641 acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg
     8701 ttcttaatgt atgctctgac tgcagcatac atcagcggtg ccggtgaatt gttggcctcc
     8761 agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact
     8821 tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt taaccgtttt
     8881 ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt
     8941 cacaaagtga atcttttaac cctgccgttg caacaggggc tggcactgtc tgcaatcccg
     9001 gtgattttta cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat
     9061 ggcaacattc gtaagctacg ctgggtgttt ataatcggta gtgccatccc cctggtggca
     9121 tatattttct ggcaggtggc gacgcttggc agcattgatt caacaacctt tatgggattg
     9181 ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat gatggcctct
     9241 ccgcatgttg agctggcggt gcatttattt gctgatttag ccctcgccac gtcatttctc
     9301 ggcgttgcgt taggcttatt tgattatctg gctgatttgt ttcagcgttc aaataccgtt
     9361 ggtggacgat tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt tgcactgttt
     9421 tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca
     9481 ttgattatcc cttcactatt gacctggcaa agcagaaagc acaatcctca ggcgggttac
     9541 cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt
     9601 ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc
     9661 aaattcctta ttgggtgcca gaattaacgc tgacacccaa tgtgacctct taatgcaggc
     9721 agcactgctt aaatttctta ccactgccgc acggacaagg atcgttacgc cccggtttct
     9781 cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtatgcat
     9841 gtaaatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca
     9901 ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg
     9961 gttttaaaga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa
    10021 agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg
    10081 gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag
    10141 ccaggttcat aaagcgcgtc atctctttct ctgacgccca acgcggcaca tagtcagccc
    10201 caccccacac ggcaaccagc cactgttctg gttcaatctc ttgcggagaa ctcaacaccg
    10261 ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg ttgtatttgg
    10321 tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct
    10381 tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata
    10441 ctttttgagt ggctttgccg gtgattaaaa attaaggagg gtgtaacgac aagttgcagg
    10501 cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attactttga ttttattctt
    10561 atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt
    10621 ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt
    10681 tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc
    10741 gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg
    10801 agcgggaaac gagactcgaa ctcgagacct cgaccttggc aaggtcgtgc tctaccaact
    10861 gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca
    10921 atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat
    10981 ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa
    11041 tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca
    11101 aagaaaagtg caataccgac gtactcaacc caaatgttcg gacgccacag cagccaggcc
    11161 aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacggc cacgctactg
    11221 cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc
    11281 atcgttgccg ccggtaatgt cacccaccag ctgtgataat gctcggttac cagcaccatg
    11341 gcgatagcca cgagaacttt atctgccaca gggtcaagga aagcaccaaa ccgggtactc
    11401 tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa
    11461 atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat
    11521 gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg
    11581 ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca
    11641 acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct
    11701 cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac
    11761 gttttggccc gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccgtt
    11821 ttttacggtg cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc tggataacat
    11881 gcagcgcggg tgaatctggc ggcaaactaa atccctcacc ttccggctca aagaacagcg
    11941 tttccagtcc agccttacga tctgctcctt tggcaacgcc aagtagcagc ggatgatttt
    12001 tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc
    12061 cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc
    12121 gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat
    12181 agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg
    12241 tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt
    12301 tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc
    12361 tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc
    12421 gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca
    12481 gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc
    12541 tgccttgtaa atagaactga cctacgaagg tttctaccac ctcgctcagt tccgtaccgc
    12601 caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata
    12661 cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcaagg tcgtcgccgg
    12721 tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac
    12781 atgccgcttc ttcaaactcc agattctggc tggcagtttc catacggcta atgagttgcg
    12841 taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt
    12901 attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct atctggtatt
    12961 gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa
    13021 tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat
    13081 attcaccttt ggcatgcttt gcaccacgat gcatcgccag acgcgggtgg gtatcgccac
    13141 tcaggaagat aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat
    13201 agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtatgc gtaaccgtta
    13261 catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctgc
    13321 ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac
    13381 caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt aaaaacgctt
    13441 ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg
    13501 aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata
    13561 gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt
    13621 ctggccctta gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg
    13681 gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga tatcagaagc
    13741 aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct gcggagccgc
    13801 gcctttgctg aggtagccag cagcacctgc ctgcatgact ttcgctggta aagggttttc
    13861 tgtatggacc gtaagcatga tgattttaac atcagctgtg gaacgcgcga ttttacgcgt
    13921 cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt
    13981 tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc tctccgacga cttttatacc
    14041 cttaatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag
    14101 tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca
    14161 ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt
    14221 tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc
    14281 ttaccggatc gtttcacagt aagccctacg atgaaatgtg acaaaaattg actttattca
    14341 gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca
    14401 aaaaacattc atgataaaaa tatttattgg tcattatatt aacgtatttt atagcactgc
    14461 cgatatcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc
    14521 cgccttaaca tttttcacca caaattttca ttgcaacatg acgaggaaaa taaatgagta
    14581 cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga
    14641 aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt
    14701 taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca
    14761 aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgagagcgtg
    14821 cctttcagcc ggttattgac aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg
    14881 tccatttcag aatttctttt tcgcgcttgc tgaaattcat ctctggcgtc atcactattt
    14941 catcatttaa acgcatcaga gccatcagac tttcgcgcac cagtaactgc atttttaatt
    15001 gcagttcatc actaagaatg ggtatttcgc gcgtgctgca acgggaaaag gacaaaaagc
    15061 ccagcgcccg atttggcagc attaaatact gagtgacacc gcggcgtaaa ccatgtgcgt
    15121 gcgcggcttc ccataatggc tgtgcttcgc tgaataagtc atcattccac attaaatggc
    15181 cctgactaaa gttttcaggg ttcagcacag gatcaattgc gagaaaattt tttgcctgat
    15241 aataactaac ccacgactca gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg
    15301 gtaccgggtg gcgaacacat aacgaatagt aatcgtactc cagctgctga gcctgaagct
    15361 ctatttcatg gtaggcctct tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc
    15421 gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg caacgcccct gagagtgagt
    15481 atcatttata atgataattc aaactatcac ataaataaga tttatatata atttatatta
    15541 ttcaggcaat gaattacttt tgcaagccat cgcattctct tatgttatta atgagttatg
    15601 ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg
    15661 tcggggctga ttttttctta ttattgcagc agaagcttct cgaggaactg gcgggtgcga
    15721 ggctgctggg gatcggcaaa taacgctttt gctgccccct gctcgactat ccgcccctgg
    15781 tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg tgtcacaatc
    15841 accatcgtgc gcttttcctg cgccagctgg cggatggtgt tcaggacttc gcccaccagc
    15901 tctggatcca gcgccgacgt tggctcgtca aacaaaatca cctcaggacg cattgccagt
    15961 gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca aacgacgtgg atagctggtt
    16021 tctttaccgg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct
    16081 ttcggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac cgtacgatgc
    16141 ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc
    16201 gatttttgct gacttaatga acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt
    16261 cccgcttccg gttgttccag cagatttatg ctacgtagca atgtggtttt gccggaacca
    16321 ctcggaccga taattgccac cacttcgcca ggctttacct cgaggtcgat accgtgcagc
    16381 accgtctgac cgtggaattt tttcaccagg ttcttaactt caatggcact cattttggtt
    16441 ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat aacacagtcg
    16501 ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg
    16561 aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg
    16621 tatcttttac cagactgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct
    16681 gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat accaatactg gcagcggctt
    16741 cccactgacc tttatcaata gaagaaatgg cggcgcgcag cgtttcggcg gcataggcgg
    16801 cagtgttcag cgacagacca atcattgctg acggaatggg atctaattcg ataccaaact
    16861 gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag cggcgtacca cgaaaaatag
    16921 agatataaaa acgcgccagc cagcgcacgg gccagattgg cgacaggcgc atcagcgcga
    16981 gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat actgagttgc agggtatacc
    17041 cagccccttt caacaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga
    17101 gcgttcccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc cgccggtatg
    17161 aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt caatcagccc
    17221 cgccatcgct tttccggtat acacaggatc aagcagaatg ccttccagac gcgccagcaa
    17281 tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc
    17341 ccagagtaaa atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttgtag
    17401 gttaaccact ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc caatcagttc
    17461 gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc cggcactgcc
    17521 cgatgcgact accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag
    17581 cgcactctcc acataaccta gcgcgccaag agcattagaa ccgccaaccg gaatgacata
    17641 cgggcgaaag ccttgtgctt cgactcgcgt cgccagttct tccagttggg cattgggatc
    17701 ggtcagtgcg tcgcacattt caatctgggt attgaacaga tccagcaaca aacgattgcc
    17761 gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg ccacgcagtg
    17821 cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca tggttagact ggatcgcgcc
    17881 ggcagtaatc agcgtatcgg caccttcgcg cagggcatct gccgcgagaa attccagctt
    17941 acgtaattta ttgccgccca ttgccatggg ggtgacctca tcccgtttga tgaaaatttc
    18001 ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa
    18061 ctccaaccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg
    18121 ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc
    18181 gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa
    18241 agagcttgca gagtgccgtc tttttgcatt tccgcaattg catcattcac cgctttcagc
    18301 aggtcttcat ttcctttacg cagcgccacg ccagactcct gacgggagaa tgcttcaccg
    18361 gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag acgatcaacg
    18421 aggatcgcat cgatacgccc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag
    18481 gtacgcacat cgacaccctg aacattctgc cgcagccact cttcatagtt ggtgcccaga
    18541 ccgacaccca cttttttgcc tttcagatca gcagccgttt taatggtgcc ttcgttacct
    18601 tttttcacca gcgcctgaat accggaaatg gtgtaagggg tagagaaatc gtattttttc
    18661 ttgcgctcgt cagaaatggt gacctgatta atcaccacat caatgcgttt agagtccagc
    18721 gacgccagca taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt
    18781 gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa
    18841 ctgaacggcg gataagtgcc ttccagccct accagcagcg tgccgctctc tttgacttta
    18901 ttaagcagac cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgccacggcc
    18961 atcacaccca tcaatgcctg acgtcccaga tgtgctaatt tcatattcac cccgaatgtt
    19021 gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat
    19081 cttattctta tttaatatat atcagaagca ggcaggctgg aggatttctg cacaagtccg
    19141 gtacaggttt ggcgctgata gtgttgatac ttccgtaatg cgatgcggta attgttggtg
    19201 ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg
    19261 agggaaatat tttgctcatg tagatgatta cccaaacggc gcagacgaac gacatattca
    19321 cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa atttcttaaa gccgataata
    19381 tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca caatgccgcc
    19441 catgattgtt gttccgtttg ccagcctttt tcatcaagca gcgtgtccag gcgggaaatc
    19501 tcgattttat tcactatttt gccgtcgcga actaaggtaa tgcgatcgag taatttacgg
    19561 caatgcgcgc aatgggtctg gctgtgttta aagtctttaa gatagcggct taatggccgt
    19621 cttttcaggt gctgcaccat cattaagaac tcctggtagt caaagttaaa gtgcggcatt
    19681 tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac
    19741 tgactgaccc gcgattcccc gacctccagc accgcgccaa tctctttgag attcagctct
    19801 tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatggcttcc
    19861 atcacccgct ggcgcagatt actgtccagc agttgttgta gcgggttttc tcgctgatga
    19921 tcatcagtaa ccagttcgat gctatcgccg tgctcttcac gccactcatc gtaggagaag
    19981 agctggctgt tattggtgtc gagcagcatt tgacgataat cggcaatatc gatccctaaa
    20041 cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag ttgccctatt
    20101 gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga
    20161 agttcatcca gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa tgccgttcct
    20221 tgtagggcgt catagcgttc gacagcatta agtaacccaa tgccgcccgc ctgtagcaga
    20281 tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc
    20341 agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt
    20401 gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca
    20461 gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt tacgcaaaaa
    20521 gccgaatgta aaaaaccccg ccggtggcgg ggtttgagag gcattcagac gattaaccct
    20581 gcagcagaga cagaacctgc tgcggtacct ggttggcttt tgccagcacg gagttaccgg
    20641 cctgctggat aatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct
    20701 gaatacggga ctgcgcttca gacaggttgg tagtggtgtt gttcaggttg gtgactgcgg
    20761 aatccaaacg gttttgcacc gcacccaggg aggagcggaa tttgtcgatg gagctgatgg
    20821 cttcatccag agctttcagg ggatcggctg tcgtagctgc ttttgtttca gcatctgtcg
    20881 tcaatttacc atcagcatct ttataaactg ccttaccgct gccgttagtc actgaaccat
    20941 catcctgtag gtaaaaatct acagtatcag atgtggtcaa cgcctcgttg ttatcaacat
    21001 aaaccgaatc aacacctttt gttacagcgc cagttgtgcc gttgacgcta taacctgcag
    21061 tagtgaaatc aacattgttt cccgaggttg atacagcttt atcgaaagct gcctgatcaa
    21121 cttttgcacc tgttactgaa taagtaatag tgtccggcgt agtactatta ccggtaaagg
    21181 agatagaggt accgccaatg tcaatggttg tcgctttggt attattggct gcaagtgtgg
    21241 acagactagc ctcagtcaaa gaagcaccag agccgttttt cgttaagcgc ccagttgtat
    21301 caatgtagag tttatctcca ttgcttgccg taattttacc atcactgctg atctgaacat
    21361 cctgtgcaga accatcgata gtaatagtgc ccttagcggt atcaccaact cccggggtca
    21421 aatatttctg aacatcggca gctgaagccg ttgtggtatc aaaagagtat gacttacttg
    21481 cactgtcata cttgtaattc gttgcactag ccgccgttcc gaagccgtta ttaatggttg
    21541 cctgaacagt atcaccatca ctaactccag ccagcaaatc agacgcttta gcagcatcgt
    21601 aacccgcact cacagtatat ttgttgccga ccactgttgc attagcagct accaagtcag
    21661 ctttagatgc agcagtatta gccacagccc cgccaccatt cacattaaac ccattcagcc
    21721 ccagcgtatc agagtcaatt ttcttcagat caatagtgat agtctggccg tcattcgcac
    21781 caacctgaat tttcatcgaa ccgtcttttg ccagtacgtt cacgccgttg aactgggtct
    21841 gaccggatac gcggtcaatt tcgtcgagac gggatttgat ttcgtcctga atggagtcca
    21901 gatccgaatc agagttagtc ccggtagaag cctgaaccgt cagctcacgg atacgctgta
    21961 agttgttgtt gatttcggac agcgcgcctt cagtggtctg tgcaacagaa ataccgtcgt
    22021 tggcgttacg tgcagcctga gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg
    22081 cctgacccgc ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga
    22141 tagaactcga cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc
    22201 tgttggtatt aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg
    22261 ttttgggttt ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac
    22321 aacctttaga atttttttca caaacagcca ttttttgtta gtcgacgaaa tactcttttc
    22381 tctgcccctt attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcagg
    22441 ccgataaccc cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta
    22501 tttcatcgct gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg
    22561 ccgcgcaaaa agcgacgcta acccctattt caaatcagca atcgtcgttt accgctaaac
    22621 ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat
    22681 tgtctaaagc cgatcttttt tccgccacca gcaccaccag cagcaccacc gcgttcagtg
    22741 ccaccaccgc gggtaacgcc atcgccggga aatacaccat cagcgtcacc catctggcgc
    22801 aggcgcaaac cctgaccacg cgcaccacca gagatgatac gaaaacggcg atcgccacca
    22861 gcgacagcaa actcaccatt caacaaggcg acgacaaaga tccgattacc attgatatca
    22921 gcgcggctaa ctcatcgtta agcgggatcc gtgatgccat caacaacgca aaagcgggcg
    22981 taagtgcgag catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg
    23041 acaccggcct tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt
    23101 ttatgggcta tgacgccagt gccagcagca acggtatgga ggtctcggtc gccgcccaga
    23161 atgcgcagct gacggtcaac aacgtcgcca tcgagaacag cagcaacact atcagcgacg
    23221 cgctggaaaa catcactctg aacctgaacg atgtcaccac gggcaaccag acgctaacca
    23281 tcactcagga cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctataact
    23341 cgctaataga taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata
    23401 gccagagttc tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc
    23461 agttgaagtc gatgctgagt aataccgtca gttcttccag ctataaaacg ctggcgcaga
    23521 ttggtatcac gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg
    23581 ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa
    23641 ccggcatcac aaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta
    23701 ttaaagccgc taccgatggc gttagtaaaa ccctgaataa gttaactaaa gactacaacg
    23761 ccgccagcga tcgcattgat gcgcaggtcg cgcgctacaa agaacaattt acccaactgg
    23821 acgttttaat gacctcgtta aacagcacca gcagctactt aacgcagcag ttcgaaaaca
    23881 acagtaattc caagtaatca ttattcatcg ggagacaggt catgtacgcg gcaaaaggca
    23941 cccaggccta tgcacaaatt ggcgtcgaaa gcaccgtaat gagcgccagc caacagcagt
    24001 tggtcaccat gctatttgat ggagtgctga gcgcactggt tagagcgcga ctgtttatgc
    24061 aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga
    24121 acggactgcg ggtgagtctt gatgaagaga gcaaagacga attaacccaa aacttaattg
    24181 ctctttatag ctatatggtc aggcgtttgc tgcaagccaa tttacgcaac gatgtctccg
    24241 cagtcgaaga ggtggaagca ttaatgcgta atattgccga tgcctggaaa gagtctttac
    24301 tctccccttc ttcgattcag gacccagtct gatgaacaat gcaccgcatt tgtatttcgc
    24361 ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg
    24421 ggacgaactc atcgctagcg aaatggcgta tgtgaatgcg gtgcaggaga tcgcacacct
    24481 gactgaagag gttgccccgt ccactacgat gcaggagcag ctccgcccga tgctgcacct
    24541 gattctcgac aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc
    24601 gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg
    24661 cggctttgtg ctggctccgc aggataacct cttttgaatc ttaatgagcc gatggctcgc
    24721 gaataatccg attacggcta cgcttctaat attccccttg aatggagtcg aagaatgcgt
    24781 aatcccacgc tgttacaatg ttttcactgg tattacccgg aaggcggtaa gctctggcct
    24841 gaactggccg agcgcgcaga cggttttaat gatattggta tcaatatggt ctggttgccg
    24901 cccgcctata aaggcgcatc gggcggctat tcggtcggct acgactccta tgatttattt
    24961 gatctaggtg agtttgatca gaaaggcagc atccctacta aatatggtga taaagcacaa
    25021 ctgctggccg ccattgacgc cctgaaacgt aatgacattg cggtgctgct ggacgtggta
    25081 gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg tgtaaatgct
    25141 gatgaccgta cgcaaattga cgaagaaatc attgagtgtg aaggctggac gcgttacacc
    25201 ttccccgctc gtgccgggca atactcgcag tttatctggg atttcaaatg ttttagcggt
    25261 atcgaccata tcgaacaccc tgatgaagat ggcattttta aaattgttaa cgactatacc
    25321 ggagaaggct ggaacgatca ggttgatgat gaactcggta atttcgatta tctgatgggt
    25381 gagaatatcg attttcgcaa tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg
    25441 gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg cggtcaaaca tattccggca
    25501 tggttttata aagagtggat cgaacacgta caggaagttg cgccaaagcc gctgtttatt
    25561 gtggctgaat gctggtcgca tgaagtcgat aaactgcaaa cgtatattga tcaggtggaa
    25621 ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat tccatgaagc atcgcgcatg
    25681 ggtcgcgact acgacatgac gcagatcttc actgggacgc tggtggaagc cgatcctttt
    25741 cacgccgtaa ccctcgttgc caaccacgac acccaaccgt tgcaagccct cgaagcaccg
    25801 gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt tgttgcggga aaatggcgtt
    25861 ccttcggtgt tctatccgga cctctacggt gcacattacg aagatgtcgg tggtgacggg
    25921 caaacctatc cgatagatat gccaataatc gaacagcttg atgagttaat tctcgcccgt
    25981 cagcgtttcg cccacggtgt acagacatta tttttcgacc atccgaactg cattgccttt
    26041 agccgcagtg gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa cggagatgat
    26101 ggcgaaaaaa ccattcatct gggagagaat tacggcaata aaacctggcg tgattttctg
    26161 gggaaccggc aagagagcgt agtgacagac gaaaacggcg aagcgacctt cttttgcaac
    26221 ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt aaatccatcc ccggcggcaa
    26281 gccggggaga tttcattacg gcagtggcgt cggtaacggc gctttatcca gcgcggcggc
    26341 acactctgcc gtcgggcgat ccacacgctc catcgtcata ccatcgtact caatggtgtt
    26401 accgtcacgc tcaacctcgt acagctcacg ttttacggta acgttagtga gatcatcaga
    26461 catcaatgtc aacttacccg gcacggcaat cccccgctgc cactgacggc aatccaaggt
    26521 atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc ggactgacca gcgcacgttg
    26581 cggccctttg gtctgccagt agcccgccag ccagtccggc gcgggggttt tcacgacatt
    26641 gttatagttt tcgacttcgg cacagcccgc cagcaacatg agcgcgcctg caattgcgag
    26701 ttttttcatc attcgtcctg catgagaaga aaagaggatt gtggcattaa agctctgatg
    26761 tcgccagcct ttccaggttc agagagaaat ttgatctaac tattattttt gtgaattatt
    26821 accccgaaaa taaatttgca tggtgtatga ttcgcggctc ttgtttcaaa ccttctgagt
    26881 tcagaggcta cattcatgtc atggcagcaa ttcaaacacg cctggttgat taaattctgg
    26941 gcacccatcc ctgcggtcat cgcggcgggt attctctcca cttactattt tggcattact
    27001 ggcacctttt gggctgtcac gggtgaattt acccgttggg gcggtcagct cctgcagctg
    27061 tttggcgtcc atgctgaaga gtggggttat tttaaaatta tccatctgga aggatcgcca
    27121 ttaacccgca tcgacggcat gatgatcctc ggtatgtttg gcggctgctt tgctgctgcg
    27181 ctgtgggcca acaatgtcaa actacgcatg ccacgcagcc gtatccgcat tatgcaggcc
    27241 atcattggcg gcattatcgc cggttttggc gcgcgtctgg caatgggctg taacctggcg
    27301 gcgttcttta ccggcattcc tcagttctcg ctgcatgcct ggttctttgc catcgccact
    27361 gccattggtt catggtttgg cgcgcgcttt accctgctgc ccatcttccg tattcccgtg
    27421 aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt
    27481 cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg gctgggcgct gctcacggcg
    27541 atgaaccaac caaaactggg gctggcaatg ctgtttggcg tcggctttgg tttactgatt
    27601 gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata tgtggatcac cggacgtacc
    27661 catatggcga aagcaatcat tatcggtatg gcggtgagtg ccatcgggat cttcagttac
    27721 gtacagttag gcgttgaacc taaaatcatg tgggcgggac caaatgcggt aattggtggt
    27781 ttactttttg gttttggcat cgtgctggca ggcggctgcg aaaccggctg gatgtaccgc
    27841 gcggtagaag gccaggtgca ctactggtgg gtcggtctgg gcaacgtgat cggctcaacg
    27901 attctggcgt attactggga tgatttcgct ccggcgctgg ccaccgactg ggacaaaatc
    27961 aacctgctga aaacttttgg tccgatggga ggcctgctgg tgacatattt gctgctgttt
    28021 gctgcgctga tgttgattat cggctgggaa aaacgcttct tccgccgtga ggcaccgcag
    28081 actgctaagg agatcgcatg aaaaatatcg ttcctgatta ccgccttgat atggtgggtg
    28141 aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat gccgcaatta aaaaaaggtg
    28201 agatcctgga agtggtgagc gactgtccgc agtcgatcaa taatattcca ctggatgcgc
    28261 gtaatcacgg ctacacggtg ctggatattc agcaagacgg gccgaccatt cgttatttaa
    28321 ttcagaagta atccctcatt cccgctgtgg cccacagcgg gaacatcgtt acctctgaca
    28381 catagatttt acccgccccc tctcctattc ttttcagtat gatctcagga ggttgctatg
    28441 tgtggacgct ttgcccaatc ccaaacgcgt gaggattacc ttgcgcttct cgcggaagat
    28501 attgaacgcg atattcccta cgatcccgaa cccattggca gatacaatgt cgcgccggga
    28561 accaaagttc ttctgctcag tgaacgtgat gaacaccttc atctggatcc ggttttctgg
    28621 ggatatgctc ccggatggtg ggataaaccg ccgctgatta acgcccgcgt agaaactgcc
    28681 tcaaccagtc gcatgtttaa accgctctgg caacatggtc gggcaatctg ttttgccgat
    28741 ggctggtttg agtggaaaaa agaaggcgac aaaaaaagcc ttattttatc tatcgcgctg
    28801 acggacaacc tgtttttatg gccgcgatag gtagcatacc gtttgagcgt ggtgacgaag
    28861 ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg tctggtagat attcatgacc
    28921 gccgcccgct ggttctgtcg ccagaagctg cacgggaatg gatgcggcaa gacattggtg
    28981 gtaaagaagc ctcagaaatc gctaccagaa gctgtgtgcc agcaaaccag ttcacctggc
    29041 accctgtgtc gcgcacggtg ggtaatgtca aaaaccaggg cgcggagtta attcaacctg
    29101 tttgttgaca tcctccacgc cctgaatgac gaggacccct gctacgttca ggctgtcgcc
    29161 tgaatcattt cggtgggttc ctgcttcaac gagcggcctg actgcaccat ctctccacag
    29221 gcaagaacgg cgtgccccgc cgctaaaatg ttacgagctc catttacatc ggcgttcgct
    29281 gtatatccac atacctggca tctgaattta ctttgtgaca ggcgattttc tttcgctgta
    29341 tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg gaacagcaag cacctgaccg
    29401 ccacgccaga gctgcttgta ctcaagctgg cggcgcattt cataccagcc ctgatccagt
    29461 atcgaacggt ttaaacctga ttttgcccgg acattgcgcc ccggctggct gaccgtaccc
    29521 gctgctgact ttgacatgtt gctgaccttc aaatcctcaa tgactatcat tacgtggttt
    29581 ttgctgacgg tcgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa
    29641 tgcagtcgct gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg
    29701 cttaactgtc gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc
    29761 tcaaagactg tgccatctga cagcgaggca agtttagcca cgccagcatc cagcccgacc
    29821 attgatgctg aagggtgaac cggagttgat acttcacttt ctgcctgaat actgatgtac
    29881 cacttaccgc aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggctg
    29941 ttccggtagc gcatccagcc aagttttggc agaaaaatac ggctgttttc ctgagcgagc
    30001 ttaacgccct gcggatagcg gaatgcatca ttctgtcacc gctttttgaa tcaggggaac
    30061 gccgagcgct tctggaaaaa gtttttgcag gcctgctcag ggtctttcag tgactgttgc
    30121 aatggctgtg acggagcatc tttaatccat tgcgtttcag tggcattttt ccactcaacc
    30181 aatcaggaag ccattttcgc gtaagggatg tctttgttac cggccttatg attctcattc
    30241 tgacgtgcca gagtacgact gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
    30301 cgcgcctgtt gaccaccggg tcttaaccgg aatttgaata cctgtagtcg tttcacaggg
    30361 caaattatac tttggtctgt gaaaaaaact gatatccgac gcggaaggca ctgtgttttc
    30421 ctgatgcatg ctcaccgggt ctttgttacc aa
//
LOCUS       DAJTOW010000040        30297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTOW010000040 DAJTOW010000000
VERSION     DAJTOW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.040"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO244_001943"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO244_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO244_001944"
     CDS             complement(1023..2045)
                     /locus_tag="OO244_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5663777.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO244_001945"
     CDS             complement(2059..3561)
                     /locus_tag="OO244_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5663778.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO244_001946"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO244_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO244_001947"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO244_001947"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5663779.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO244_001948"
     CDS             complement(5877..6218)
                     /locus_tag="OO244_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5663780.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO244_001949"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO244_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5663781.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQGPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO244_001950"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO244_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5663782.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO244_001951"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO244_001951"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5663783.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO244_001952"
     CDS             12897..14240
                     /locus_tag="OO244_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5663784.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO244_001953"
     CDS             complement(14302..14508)
                     /locus_tag="OO244_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5663785.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO244_001954"
     CDS             14833..15387
                     /locus_tag="OO244_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5663786.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO244_001955"
     CDS             complement(15450..16388)
                     /locus_tag="OO244_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663787.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO244_001956"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO244_001956"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5663788.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO244_001957"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO244_001957"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5663789.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO244_001958"
     CDS             complement(19599..19979)
                     /locus_tag="OO244_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5663790.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO244_001959"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO244_001959"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5663791.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO244_001960"
     CDS             21037..22002
                     /locus_tag="OO244_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5663792.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO244_001961"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO244_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5663793.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO244_001962"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO244_001962"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5663794.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO244_001963"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO244_001963"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5663795.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO244_001964"
     CDS             25376..25921
                     /locus_tag="OO244_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5663796.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO244_001965"
     CDS             complement(25905..26552)
                     /locus_tag="OO244_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5663797.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO244_001966"
     CDS             26764..27555
                     /locus_tag="OO244_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5663798.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..28389
                     /locus_tag="OO244_001967"
     CDS             27565..28389
                     /locus_tag="OO244_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5663799.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINGTVANSRW"
     gene            28426..29130
                     /locus_tag="OO244_001968"
     CDS             28426..29130
                     /locus_tag="OO244_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCR5663800.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            29338..29564
                     /locus_tag="OO244_001969"
                     /pseudo
     CDS             29338..29564
                     /locus_tag="OO244_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(29566..>30297)
                     /locus_tag="OO244_001970"
     CDS             complement(29566..>30297)
                     /locus_tag="OO244_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5663801.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHKHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
BASE COUNT         7467 a         8005 c         7485 g         7340 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgtactgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc gagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggcct tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acggcactgt tgcaaatagt
    28381 cggtggtgat aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa
    28441 ggccggcatt ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc
    28501 atcagttacc gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc
    28561 acgatttacc gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac
    28621 tggcgtaacc cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat
    28681 ggccgctggg cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat
    28741 ctctcctccc gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaac
    28801 gtgaagaagt ggcagatccc gcgattcatc aacacggata aagcgcccgc ctatggtcgc
    28861 gcgcttgctc tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt
    28921 aagtaccgga acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc
    28981 acgctgggat ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg
    29041 cgtgcactac gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg
    29101 cgcctggtaa gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa ggctgcctca
    29161 tcgctaactt tgcaacagtg ccatgcaggg ttgtgagatg aattcaaacc ggctggcagc
    29221 gccagtgata ttcgaagatt cgtccggttg ttacccagta tgcattaaaa acccagacat
    29281 tatggacaat ataacggctt ctacaacaag gaagtgcctg tttaccatcc gggtgtaatg
    29341 actcaccttt gtgggctttt gcccgtcgcg ctcttaacgt tgaagacatt ctctgcctgc
    29401 cgattcagga ctgcgacaaa agccatatct ggctactggt caaggatgac cagcgtctgg
    29461 agtaggtgat aagccaaata gataagctgg aatatgtggt gaaagtgcaa cgtaatcagt
    29521 ccaacccgac gatgtttaac aagattgtgg tgttttttca gtaagtcatt cgtcttctga
    29581 atcaggtgta atgatcttta cagaaccatt ctggcagtgc gtaacataat ctgccggtaa
    29641 cggacgatcg ctaatcagaa cgtcaaatgc agagatcggt gcgaggctgg cagaggccac
    29701 ttcatcgaac aacgcgtggc gggcgagcaa aattttccgt agtccacggt tcatcacttt
    29761 acgcttagtg gcaagatctt cagggttaaa ccagctgacg ccaaagtgct tatgcacacc
    29821 gctggcggaa ataaatattt ttcgcggatt gagagagtct aatggcgaag agttactggc
    29881 atcgtaaaaa gcatcacttc tggcacgata cgtaccacca caaagtattg ctgttacatt
    29941 aggcttttca ttcaacgcaa caaagacgcg atgtgagtaa cagatgccgg tgaaggtgat
    30001 tgcatccggg atcatgctta taacgagtgg tatctcctgg ccattatcaa agaagatcag
    30061 atcattttca ttaaccattc cggcagccag aattgcaata ggtaagtcat cacgatgatt
    30121 ttttggaacg tcatggatta ctggcatgga tggcgcgggt ttattcacca ttacaatata
    30181 gccacccagt agggtcagtg gcagaggttc atcttcctga tggagatcgc gacgaatagt
    30241 cattacagaa acttccagca ttcgcgctgc gtctttcaaa tggattctgt cggtttt
//
LOCUS       DAJTOW010000041        30096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTOW010000041 DAJTOW010000000
VERSION     DAJTOW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.041"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO244_001971"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO244_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5663802.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO244_001972"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO244_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5663803.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO244_001973"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO244_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5663804.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO244_001974"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO244_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5663805.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO244_001975"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO244_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5663806.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO244_001976"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO244_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5663807.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO244_001977"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO244_001977"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5663808.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO244_001978"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO244_001978"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5663809.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTILPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO244_001979"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO244_001979"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5663810.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO244_001980"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO244_001980"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5663811.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO244_001981"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO244_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5663812.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO244_001982"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO244_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5663813.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO244_001983"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO244_001983"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5663814.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO244_001984"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO244_001984"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5663815.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO244_001985"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO244_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5663816.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO244_001986"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO244_001986"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5663817.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO244_001987"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO244_001987"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5663818.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATIFAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO244_001988"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO244_001988"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5663819.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO244_001989"
     CDS             21622..22212
                     /locus_tag="OO244_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5663820.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO244_001990"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO244_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5663821.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO244_001991"
     CDS             22684..23379
                     /locus_tag="OO244_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5663822.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO244_001992"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO244_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5663823.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO244_001993"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO244_001993"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5663824.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO244_001994"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO244_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5663825.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALENSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO244_001995"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO244_001995"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5663826.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO244_001996"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO244_001996"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5663827.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7059 a         7840 c         8274 g         6923 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gattactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct agatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgacca ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatat ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagatcagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctgga gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    30061 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAJTOW010000042        29661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTOW010000042 DAJTOW010000000
VERSION     DAJTOW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.042"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            6..800
                     /locus_tag="OO244_001997"
     CDS             6..800
                     /locus_tag="OO244_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5663828.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            822..2231
                     /locus_tag="OO244_001998"
     CDS             822..2231
                     /locus_tag="OO244_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5663829.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2209..2988
                     /locus_tag="OO244_001999"
     CDS             2209..2988
                     /locus_tag="OO244_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5663830.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2985..3845
                     /locus_tag="OO244_002000"
     CDS             2985..3845
                     /locus_tag="OO244_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5663831.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3993..4568)
                     /locus_tag="OO244_002001"
                     /pseudo
     CDS             complement(3993..4568)
                     /locus_tag="OO244_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4585..4845)
                     /locus_tag="OO244_002002"
     CDS             complement(4585..4845)
                     /locus_tag="OO244_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5663832.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4836..6107)
                     /locus_tag="OO244_002003"
     CDS             complement(4836..6107)
                     /locus_tag="OO244_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5663833.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6185..6547)
                     /gene="queD"
                     /locus_tag="OO244_002004"
     CDS             complement(6185..6547)
                     /gene="queD"
                     /locus_tag="OO244_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5663834.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6866..8665
                     /gene="cysJ"
                     /locus_tag="OO244_002005"
     CDS             6866..8665
                     /gene="cysJ"
                     /locus_tag="OO244_002005"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5663835.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8665..10377
                     /gene="cysI"
                     /locus_tag="OO244_002006"
     CDS             8665..10377
                     /gene="cysI"
                     /locus_tag="OO244_002006"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5663836.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10451..11185
                     /gene="cysH"
                     /locus_tag="OO244_002007"
     CDS             10451..11185
                     /gene="cysH"
                     /locus_tag="OO244_002007"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5663837.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11449..11601
                     /locus_tag="OO244_002008"
     CDS             11449..11601
                     /locus_tag="OO244_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5663838.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11795..12886
                     /locus_tag="OO244_002009"
                     /pseudo
     CDS             11795..12886
                     /locus_tag="OO244_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13032..14069)
                     /gene="iap"
                     /locus_tag="OO244_002010"
     CDS             complement(13032..14069)
                     /gene="iap"
                     /locus_tag="OO244_002010"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5663839.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14321..15229
                     /gene="cysD"
                     /locus_tag="OO244_002011"
     CDS             14321..15229
                     /gene="cysD"
                     /locus_tag="OO244_002011"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5663840.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15231..16658
                     /gene="cysN"
                     /locus_tag="OO244_002012"
     CDS             15231..16658
                     /gene="cysN"
                     /locus_tag="OO244_002012"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5663841.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16658..17263
                     /gene="cysC"
                     /locus_tag="OO244_002013"
     CDS             16658..17263
                     /gene="cysC"
                     /locus_tag="OO244_002013"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5663842.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLELLRQNDIIRS"
     gene            17313..17636
                     /locus_tag="OO244_002014"
     CDS             17313..17636
                     /locus_tag="OO244_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5663843.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17830..18141
                     /gene="ftsB"
                     /locus_tag="OO244_002015"
     CDS             17830..18141
                     /gene="ftsB"
                     /locus_tag="OO244_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5663844.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18160..18870
                     /gene="ispD"
                     /locus_tag="OO244_002016"
     CDS             18160..18870
                     /gene="ispD"
                     /locus_tag="OO244_002016"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5663845.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDLRADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18870..19349
                     /gene="ispF"
                     /locus_tag="OO244_002017"
     CDS             18870..19349
                     /gene="ispF"
                     /locus_tag="OO244_002017"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5663846.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19346..20395
                     /gene="truD"
                     /locus_tag="OO244_002018"
     CDS             19346..20395
                     /gene="truD"
                     /locus_tag="OO244_002018"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5663847.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20376..21137
                     /gene="surE"
                     /locus_tag="OO244_002019"
     CDS             20376..21137
                     /gene="surE"
                     /locus_tag="OO244_002019"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5663848.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21131..21757
                     /gene="pcm"
                     /locus_tag="OO244_002020"
     CDS             21131..21757
                     /gene="pcm"
                     /locus_tag="OO244_002020"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5663849.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21897..23036
                     /gene="nlpD"
                     /locus_tag="OO244_002021"
     CDS             21897..23036
                     /gene="nlpD"
                     /locus_tag="OO244_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5663850.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAAVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23099..24091
                     /gene="rpoS"
                     /locus_tag="OO244_002022"
     CDS             23099..24091
                     /gene="rpoS"
                     /locus_tag="OO244_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5663851.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24211..24618)
                     /locus_tag="OO244_002023"
     CDS             complement(24211..24618)
                     /locus_tag="OO244_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5663852.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24765..25358
                     /locus_tag="OO244_002024"
     CDS             24765..25358
                     /locus_tag="OO244_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5663853.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25358..26305
                     /locus_tag="OO244_002025"
                     /pseudo
     CDS             25358..26305
                     /locus_tag="OO244_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26578..27141)
                     /locus_tag="OO244_002026"
     CDS             complement(26578..27141)
                     /locus_tag="OO244_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663854.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27306..27557
                     /locus_tag="OO244_002027"
     CDS             27306..27557
                     /locus_tag="OO244_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5663855.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27559..29655
                     /locus_tag="OO244_002028"
     CDS             27559..29655
                     /locus_tag="OO244_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5663856.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7245 a         7358 c         8030 g         7028 t
ORIGIN      
        1 taacagtgaa aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg
       61 aagacatgaa aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt
      121 cgatcgctcg tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg
      181 gaaaatgtgt gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg
      241 aagggattgc ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga
      301 ttgaaacctg gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc
      361 agatcctcaa aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca
      421 tccacgaaat ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg
      481 acatcaccat gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact
      541 tcgtctatga ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca
      601 atccgctcaa caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc
      661 accatggcat tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg
      721 cgttgctgga agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta
      781 tctatccgat tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt
      841 tcggcttctc aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca
      901 cttcaccggt gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc
      961 tcactttcgg cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta
     1021 ttcaacttac gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg
     1081 cgctccttgg tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc
     1141 ggcaaaaaat cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt
     1201 ttgccaccac gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag
     1261 gcgattactc agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta
     1321 ttttgctggg cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg
     1381 gacatcactt tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc
     1441 tgcccgcgtt gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac
     1501 tgtgccaggg gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg
     1561 ttttactggg cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt
     1621 cgcgttactg gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat
     1681 ggtttgtgat ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc
     1741 tgactgccag cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc
     1801 tgacgcacct gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa
     1861 cgctggtagt aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc
     1921 tcttcagcac caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt
     1981 ttcctactga cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg
     2041 cggcggtaag tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct
     2101 tattgctcct agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag
     2161 aaactaaagc cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg
     2221 ttagcgttta aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg
     2281 gctcagggta atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa
     2341 caggccgctg ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc
     2401 gcgttgagca tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg
     2461 tttgaggaag ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc
     2521 gctcgccata ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc
     2581 caaagcagcg aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg
     2641 ccctgcttca cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag
     2701 cgtactgaac atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt
     2761 cagtgcggtg aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa
     2821 gcagaaatta tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg
     2881 gcaagagcag agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa
     2941 gctcaaaagc tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg
     3001 tgacaataaa tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg
     3061 gttgtacgct ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg
     3121 atgcgatggt ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa
     3181 cgtttggcga tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc
     3241 aggtgacttc gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt
     3301 taacggcgac gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg
     3361 gagcagcgaa aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg
     3421 cgcttcccga ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag
     3481 ctgaggccag acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg
     3541 ttatgctggc agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg
     3601 gtggcgttga tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt
     3661 gcattgttgt tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt
     3721 ttatggtggc gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg
     3781 tggttgatga ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc
     3841 aataacgtgc tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat
     3901 aagacgcgtt aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat
     3961 caaaacaaat tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag
     4021 actccggtat tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct
     4081 tcttcatcgc ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag
     4141 cccagcactt tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca
     4201 acttacttat caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag
     4261 aaaaaaactt ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc
     4321 gcctcggtca ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc
     4381 aaatcaacat gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg
     4441 atggtgcaga tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt
     4501 tgcaggctgg cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag
     4561 aggggcataa attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg
     4621 cggatagcgc cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc
     4681 caggcagccg cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac
     4741 cggacaggcg ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat
     4801 gtgcggcgca tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata
     4861 acatcgcccg ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc
     4921 agcggtggaa caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac
     4981 gcaggccacg cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt
     5041 tgtagaacat cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt
     5101 tgcggctcgc ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc
     5161 agcgccatat ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg
     5221 cccaccagca gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct
     5281 ggcaccagat gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc
     5341 ggatgcgctt taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa
     5401 gagagcgggc aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg
     5461 ccgggtaagt catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc
     5521 agatgaaaac gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac
     5581 gccatcgctt cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg
     5641 gcaccttctg ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca
     5701 cagactctgc cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca
     5761 ccttcttttt cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag
     5821 gattcaccgc cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa
     5881 aggctttcgt gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt
     5941 tccgcgagtg catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca
     6001 gcgcgttcga gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg
     6061 cctgcaatac cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga
     6121 tatccagaac acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact
     6181 cttatcattc gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac
     6241 ttaacagcgg cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg
     6301 ttggattttc cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg
     6361 gcttaaacgc cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt
     6421 ccccggtaat ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc
     6481 cttccgggac atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg
     6541 tggacatcat aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt
     6601 ttttcgcatt atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg
     6661 ctaaattcat ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct
     6721 aaaacaggtt agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt
     6781 caccccgtta accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc
     6841 tttgcttact ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc
     6901 gttgaacccg gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca
     6961 gcttgcctgg gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc
     7021 agtgacgcca gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac
     7081 cggcaatgcg cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa
     7141 cgttaagctg gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct
     7201 catcgtagtg acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca
     7261 taagttcctg ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag
     7321 cctcggcgat agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct
     7381 ggcggaactg gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc
     7441 tgctgccagc gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc
     7501 ggcaccttcg caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag
     7561 caaagacgcg ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc
     7621 tgaaaaagac gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc
     7681 gggtgacgcg ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga
     7741 actgctgtgg ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa
     7801 cgaagcgcta cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta
     7861 cgccacgctt acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca
     7921 gcattacgcc gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga
     7981 tgccgaagcg ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc
     8041 ctcgcaggcg gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt
     8101 ggaaggccgc gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga
     8161 gggcgaagtc cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga
     8221 aaccccggtg attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca
     8281 gcaacgcgcc gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt
     8341 tacggaagac ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac
     8401 gcggatcgat ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact
     8461 gcgcgaacag ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg
     8521 cggcgacgct aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga
     8581 atttggtggc atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg
     8641 ccgttatcag cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa
     8701 ggaaaactga cagacgccga gcgcatgaag cttgaaagca attacctgcg cggcaccatt
     8761 gcggaagatt taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc
     8821 cgcttccacg gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag
     8881 aagctggagc cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc
     8941 accaaacagt ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt
     9001 cgcctgacca accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg
     9061 gtgcaccaga tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac
     9121 cgtaacgtac tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag
     9181 tgggcgaaga agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg
     9241 ctcgatcagg aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg
     9301 ccgcgtaagt tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc
     9361 aacgacatga acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg
     9421 gtgggcggtg ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt
     9481 gagtttggct atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact
     9541 cagcgtgact ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag
     9601 cgcgtggggg ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa
     9661 ccgatccgcc cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt
     9721 gatgataact ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg
     9781 cgtccgctga aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt
     9841 acggcgaacc agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag
     9901 aaaattgcca aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg
     9961 gcctgcgtgt cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg
    10021 tcttttatcg acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc
    10081 gtgatgcgtg taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc
    10141 ctggtgggta aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca
    10201 cgtatcccac ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat
    10261 gaactgatag ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg
    10321 gtgcgtgcgg gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca
    10381 tcagcccggt cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag
    10441 tgaggaatct atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg
    10501 cattctggcg ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt
    10561 tgcctgggcg ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca
    10621 ggcggcggtg agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac
    10681 cgacaccggc tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact
    10741 caagctcaac ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta
    10801 cggcaaactg tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt
    10861 cgaaccgatg aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg
    10921 gcgtgaacaa tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt
    10981 taaagtgctg ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca
    11041 tggcctgaaa tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac
    11101 aacccgtaaa tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag
    11161 ggaatgtgga ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa
    11221 tgatgggggc gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg
    11281 gaaaattaac aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg
    11341 cgaggttcca gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc
    11401 gaacgtagca attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa
    11461 tatgcccttg tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta
    11521 ggtgactcgt tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc
    11581 gcttacgaat cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga
    11641 tggtttggtc tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc
    11701 tatttattat tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta
    11761 ttggctgata tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact
    11821 gaaggataaa aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa
    11881 agagggtatc gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg
    11941 ctatttaatg gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat
    12001 ttctgacaag gaacaggcag ctcaattttt cgcctggttg ctgtgctggc acgatattgg
    12061 aaaatttgcc cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg
    12121 tgccagaaaa aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca
    12181 ttatttaagt gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag
    12241 gcgagtgata gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg
    12301 tatggatgaa ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct
    12361 tgaaatcaag ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg
    12421 cgttgaactt ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg
    12481 gacagggtcg tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta
    12541 ctggcaaaag gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga
    12601 aaccgcgcct ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt
    12661 acagcaaaag gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga
    12721 agatgtgacc ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc
    12781 tgcgggtaac gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat
    12841 gtacgatcga ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc
    12901 agcggagata aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg
    12961 atgcgatgat gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat
    13021 tttcaaaccc atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata
    13081 acgtcacggc agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta
    13141 tccagtctta cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc
    13201 tgataaccat ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg
    13261 cccgctttgt cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta
    13321 ttcaaacccg gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg
    13381 tcgcgcgtta atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta
    13441 tcgccaacaa ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca
    13501 ctcatccgct tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc
    13561 acaaatcgaa taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg
    13621 acacctaaac ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca
    13681 tctgcgtcag catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc
    13741 tgcggcgctt tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc
    13801 ttgcgattat cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga
    13861 taacccattt gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt
    13921 ccggtcatgc gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg
    13981 ccaggtttag gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca
    14041 gcggtacggt ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt
    14101 taccgccata gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc
    14161 gaaatccggt gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag
    14221 gaactactca ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca
    14281 tctgatggca agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac
    14341 tcacctgcgg caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt
    14401 ctcaaatccg gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc
    14461 gcgcaaggcg ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg
    14521 gaaattccgc gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct
    14581 gctggtgcat aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag
    14641 cgcgaaacat accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg
    14701 ttttgatgcc gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg
    14761 aatctactct ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct
    14821 gtggcacaac tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc
    14881 taactggacc gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc
    14941 gctgtatctc gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga
    15001 tgacaaccgt atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg
    15061 tacgctgggc tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga
    15121 aatcatcgaa gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg
    15181 cgaccaggcg ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg
    15241 tacttgcaca acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac
    15301 ataaaagcct gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga
    15361 ttggtcgtct gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata
    15421 acgacagtaa gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg
    15481 gcctgcaagc tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta
    15541 ccgagaagcg taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata
    15601 tggcgactgg cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg
    15661 tgctcgatca aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg
    15721 tcgtggcgat caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc
    15781 gtgaagatta tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc
    15841 cgctctccgc actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca
    15901 gcggtctgac actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc
    15961 agccaatgcg cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg
    16021 ccggaatgct ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg
    16081 gtgtggaatc aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg
    16141 ccggagaagc gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc
    16201 tgctggcggc agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga
    16261 tggcggaaca gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga
    16321 cgcgtgctcg tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg
    16381 aagttgaaaa cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac
    16441 tggtgttaga tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc
    16501 tgagcaatgt gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg
    16561 cgtcatctga attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc
    16621 cgcactgggg cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt
    16681 cgtctggcat agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg
    16741 tgtagtgctg tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct
    16801 ggaggaggcg ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg
    16861 ccacggatta tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg
    16921 cgtcggtgaa gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat
    16981 ctcgccacac cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat
    17041 tgaagtgttt gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata
    17101 taagaaagcg cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc
    17161 gcctgaatcg gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca
    17221 attattagaa ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc
    17281 atgcccggtt cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa
    17341 caaataacga cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg
    17401 tcggttttat ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca
    17461 cgctgttctt ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag
    17521 tggtggggat tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca
    17581 ccctggtgac agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg
    17641 tactttcttg tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg
    17701 cacgccctgg gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt
    17761 atccccaaca gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca
    17821 gggggcagga tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat
    17881 tcgctgtggt tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg
    17941 gcactgcaag ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt
    18001 gacgatctca atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg
    18061 accaggccgg gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct
    18121 gcggggcaaa acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat
    18181 gtttgcgccg tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag
    18241 caatatctct caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg
    18301 catccccggg tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa
    18361 cttcctctgg cgaatcatcc gcaaatcacc gttgtagatg gcggtgattt gcgtgccgat
    18421 tccgtgctgg caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc
    18481 gctcgtcctt gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc
    18541 cgcacgggag ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc
    18601 aaaaatgcca ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa
    18661 tttttccctc gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc
    18721 attaccgacg aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc
    18781 cgcgcggata acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc
    18841 acccgaacca tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg
    18901 cctttggcgg tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat
    18961 tgctggcgca ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg
    19021 cggcggcgct gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg
    19081 ccgacagccg cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc
    19141 ttggcaacgt cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac
    19201 aaatgcgcgt gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag
    19261 ccactactac ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg
    19321 tggcgctact cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg
    19381 taaaccgcaa ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga
    19441 tttgggcttt gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg
    19501 ctgcaatacc cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga
    19561 agtcagcttc gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg
    19621 cgtgccgggc aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct
    19681 ggagtatgcg cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac
    19741 cctggttctg cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg
    19801 cgtaaaaggt gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt
    19861 gcagggcgcg ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag
    19921 tttttggttg tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa
    19981 aaaagcagac gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag
    20041 ctggtttgta gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga
    20101 gctgatgata accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct
    20161 ggcattcgaa caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga
    20221 aaaagttgaa gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg
    20281 gtgggatgac gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag
    20341 cgttgtcagg gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg
    20401 acggggtaca tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg
    20461 ttcaggtggt cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat
    20521 cctccctgcg cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga
    20581 ccgattgtgt ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt
    20641 ccggaattaa cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg
    20701 ccgcgatgga aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata
    20761 aacattacga cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc
    20821 cgctgcgcac cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca
    20881 aaggtattcg cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc
    20941 aagatccgcg cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg
    21001 gtccggggac cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg
    21061 tggatttaac tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag
    21121 ttggcacgca atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg
    21181 tattcaggat gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga
    21241 tgaagcgttt gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac
    21301 aatttcgcag ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc
    21361 gcgggtgctg gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt
    21421 ccagcatgtt tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct
    21481 gaaaaatctt gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca
    21541 ggcacgtgcg ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc
    21601 gctaatgacg cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca
    21661 gtatttgaaa cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt
    21721 gcgctttgtc cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt
    21781 ttcctggtta ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg
    21841 gtgccttgca cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga
    21901 gcgcgggaag cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat
    21961 ggctggcagg ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca
    22021 atgcgcctgc aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga
    22081 cgtctacagc gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc
    22141 aacaaccgca aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg
    22201 gacgcatcgt ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta
    22261 cctataccgt gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt
    22321 tccgtgacct tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga
    22381 ccttgcaggt gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg
    22441 ccgacgcagc agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg
    22501 cgtcgcaacc gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt
    22561 tgccgaacaa caagccaact gcgaccacgg tcacagcggc tgtaacggta ccaacagcaa
    22621 gcacaaccga gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct
    22681 ggccgactga gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga
    22741 ttgatatcgc aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt
    22801 atgctggtaa cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt
    22861 acctgagtgc ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg
    22921 cggggcaaaa aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg
    22981 aaattcgtta caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg
    23041 gcggaaccag gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat
    23101 gagtcagaat acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg
    23161 agttgaggtt tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc
    23221 cgaagaggaa ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta
    23281 ccttggtgag attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg
    23341 tcgcgcactg cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct
    23401 ggtggtaaaa attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga
    23461 agagggcaac ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg
    23521 cttctcaaca tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca
    23581 aacccgtact attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac
    23641 cgcacgtgag ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca
    23701 actggataag ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc
    23761 ggtagacacc ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga
    23821 aaaagagaac ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa
    23881 atggctgttc gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct
    23941 ggggtatgaa gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg
    24001 tgttcgccag attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg
    24061 gctgaatatc gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca
    24121 agcgaggctg gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac
    24181 acgtcagcgt cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg
    24241 cataaacagc tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc
    24301 accaataggt atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg
    24361 tcgccgcttg tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc
    24421 caacatttct gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc
    24481 aataccaggt ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg
    24541 taactcgtgc tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc
    24601 tttatttcgt aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt
    24661 ttagtgacga agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta
    24721 tgttaataat aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg
    24781 ggatgacagg ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga
    24841 tgccgaatgt cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg
    24901 aaacgcctta cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg
    24961 atcaggcggc gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt
    25021 gcagtatgaa aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg
    25081 cggcggacgt cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc
    25141 ttagcaccat ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc
    25201 cgccgatgcc tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg
    25261 tagcccgcgt gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat
    25321 tgccgcaggc ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag
    25381 ctttttacag gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc
    25441 tgagccggat ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc
    25501 gctgtggttt gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg
    25561 ttcctggcag aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca
    25621 gattgatgag tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa
    25681 tccagcctgg gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc
    25741 gctgtttcgc ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc
    25801 ccgcgatccg ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga
    25861 agtgaagggc aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca
    25921 tctgcataaa gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga
    25981 tccgatcatc acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat
    26041 ggcaggcgcg ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga
    26101 tgtgccgtgg ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat
    26161 cgaagggccg ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt
    26221 gcgcatcgat aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat
    26281 gccgtggacc gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag
    26341 attaaaaaag acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata
    26401 aagcgagtcc aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag
    26461 gttcttacaa tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg
    26521 atgggtgcaa gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta
    26581 tgcgctggct gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta
    26641 aatctctcta accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac
    26701 tccaactaga gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc
    26761 tccttttctc ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa
    26821 gaacattaga atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc
    26881 ttttagttca acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc
    26941 tgctatcttt tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata
    27001 attttttaaa gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta
    27061 tcgttctact aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga
    27121 accttggggt gcttttctca ttcttggttc ctttctatct aattcattga taaataataa
    27181 aattaaaaaa taaaccataa aaacaccaaa gaacccttta cagggggtct ttggtatctt
    27241 aatctttatc catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag
    27301 gaaagatgag acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg
    27361 cgctcaaaga taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc
    27421 aggattcact gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg
    27481 acgctatagg tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta
    27541 tcaggaaggc gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca
    27601 ggaatgccag ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt
    27661 ggtgacgtta gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg
    27721 cctgaaataa cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag
    27781 aacaatgttt ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag
    27841 gcaatcgaag catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc
    27901 gaaaaccagc gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg
    27961 gaaagcgccg gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt
    28021 aaaggtgagc tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc
    28081 gctaatcgta ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg
    28141 cagaacgccg ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg
    28201 gaagaaatca gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc
    28261 atgtcagaag cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg
    28321 tacatgctgg aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg
    28381 gaagtggtga aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc
    28441 acccgccgcg actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt
    28501 atgaagctga aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt
    28561 gtgatcgatg gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg
    28621 ttcgccgttg ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat
    28681 tactcggata acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt
    28741 ctgccacgcc tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc
    28801 attattgaac gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat
    28861 tttggcaagg gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg
    28921 tttaacgccc tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag
    28981 ctgccatcgt gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac
    29041 cgcccgcatt ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc
    29101 agaaaaaaac gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg
    29161 cgcgacatgt tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg
    29221 aacaatattt actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt
    29281 tatgacattc atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt
    29341 gaggcgatat ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa
    29401 caacagcagc gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa
    29461 gatgaaggta ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat
    29521 cgcccggttg gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa
    29581 gaatttgaac gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat
    29641 gatccgctgg cctgaataaa a
//
LOCUS       DAJTOW010000043        29468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTOW010000043 DAJTOW010000000
VERSION     DAJTOW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.043"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            71..352
                     /locus_tag="OO244_002029"
     CDS             71..352
                     /locus_tag="OO244_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5663857.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="OO244_002030"
                     /pseudo
     CDS             318..1395
                     /locus_tag="OO244_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1788..2129)
                     /locus_tag="OO244_002031"
     CDS             complement(1788..2129)
                     /locus_tag="OO244_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5663858.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="OO244_002032"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="OO244_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="OO244_002033"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="OO244_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5663859.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /gene="holE"
                     /locus_tag="OO244_002034"
     CDS             3531..3761
                     /gene="holE"
                     /locus_tag="OO244_002034"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5663860.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /locus_tag="OO244_002035"
                     /pseudo
     CDS             3863..4519
                     /locus_tag="OO244_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="OO244_002036"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="OO244_002036"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5663861.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="OO244_002037"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="OO244_002037"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5663862.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQIEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="OO244_002038"
     CDS             complement(7472..8131)
                     /locus_tag="OO244_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5663863.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /locus_tag="OO244_002039"
     CDS             complement(8458..8814)
                     /locus_tag="OO244_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5663864.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO244_002040"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO244_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="OO244_002041"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="OO244_002041"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5663865.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="OO244_002042"
                     /gene_synonym="eda"
     CDS             complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="OO244_002042"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5663866.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11217..13028)
                     /gene="edd"
                     /locus_tag="OO244_002043"
     CDS             complement(11217..13028)
                     /gene="edd"
                     /locus_tag="OO244_002043"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5663867.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="OO244_002044"
     CDS             complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="OO244_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5663868.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15076..15945
                     /gene="hexR"
                     /locus_tag="OO244_002045"
                     /gene_synonym="yebK"
     CDS             15076..15945
                     /gene="hexR"
                     /locus_tag="OO244_002045"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5663869.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16073..17515
                     /gene="pyk"
                     /locus_tag="OO244_002046"
     CDS             16073..17515
                     /gene="pyk"
                     /locus_tag="OO244_002046"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5663870.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="OO244_002047"
                     /gene_synonym="msbB"
     CDS             complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="OO244_002047"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5663871.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="OO244_002048"
     CDS             complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="OO244_002048"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5663872.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="OO244_002049"
     CDS             complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="OO244_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5663873.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21086..21841
                     /gene="znuC"
                     /locus_tag="OO244_002050"
     CDS             21086..21841
                     /gene="znuC"
                     /locus_tag="OO244_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5663874.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21838..22623
                     /gene="znuB"
                     /locus_tag="OO244_002051"
     CDS             21838..22623
                     /gene="znuB"
                     /locus_tag="OO244_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5663875.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="OO244_002052"
     CDS             complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="OO244_002052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5663876.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="OO244_002053"
     CDS             complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="OO244_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5663877.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="OO244_002054"
     CDS             complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="OO244_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5663878.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..25276
                     /locus_tag="OO244_002055"
     CDS             24674..25276
                     /locus_tag="OO244_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5663879.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="OO244_002056"
     CDS             complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="OO244_002056"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5663880.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25834..26574)
                     /locus_tag="OO244_002057"
     CDS             complement(25834..26574)
                     /locus_tag="OO244_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5663881.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="OO244_002058"
     CDS             complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="OO244_002058"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5663882.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="OO244_002059"
     CDS             complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="OO244_002059"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5663883.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29255..>29468
                     /locus_tag="OO244_002060"
     CDS             29255..>29468
                     /locus_tag="OO244_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5663884.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7228 a         7571 c         7235 g         7434 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tattttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctag cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgctgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tatttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caagcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgagatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc
    10501 tacaaaaatg cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca
    10561 gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga
    10621 accagccagg aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta
    10681 gccggagaaa taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc
    10741 tgcagggctt tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg
    10801 tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc
    10861 ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca
    10921 cccgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc
    10981 gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga
    11041 gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt
    11101 tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca
    11161 cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa
    11221 aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat
    11281 aattcacgtc ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca
    11341 gccagttccg cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg
    11401 atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg
    11461 atagctgacg gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt
    11521 ttgaaacacc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt
    11581 ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc
    11641 gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc
    11701 acctggttct caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt
    11761 aacacttttg tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg
    11821 tcgagtgatt tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca
    11881 agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct
    11941 gctttgagca gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata
    12001 tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag
    12061 aagtcatccc agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg
    12121 tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca
    12181 tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga
    12241 cgcgcagcgg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag
    12301 cctggcaact gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag
    12361 aaagtacatg ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg
    12421 tccactttac cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg
    12481 cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc
    12541 atcgtcagac ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg
    12601 ttatgggaca gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat
    12661 tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc
    12721 tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa
    12781 ggctggtgcg cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac
    12841 atgcttttca aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta
    12901 ccgcatgcca actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga
    12961 taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt
    13021 ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca
    13081 ttcagaggat ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag
    13141 tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt
    13201 gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga
    13261 tattactcaa actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg
    13321 ggtccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca
    13381 gtaatggagt ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc
    13441 tgaataccac gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga
    13501 ttaaaggttt ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca
    13561 aggccaggaa ctttattcag tacctggata tccacgcctt catcaggttg cagacggata
    13621 gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt
    13681 ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag
    13741 aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct
    13801 gtattgctgc ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg
    13861 gcgaagcccg cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg
    13921 cggcgcaggg acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca
    13981 gatggcggag acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca
    14041 cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc
    14101 acggtaatct caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta
    14161 gcaaaacgca gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg
    14221 taaacctggc actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc
    14281 agcgacgtcc ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc
    14341 tcgccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata
    14401 gtgatacgat ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg
    14461 acatcgagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg
    14521 gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc
    14581 cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc
    14641 agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg
    14701 accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta
    14761 acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta
    14821 cgagcttgcg aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa
    14881 gcgcggcaaa actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc
    14941 atgttatgaa aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg
    15001 cgtaatatct gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt
    15061 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
    15121 aaatcagagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
    15181 agtattgctg ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
    15241 cgcagcatgg acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg
    15301 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg
    15361 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
    15421 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
    15481 ttaggctctt ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
    15541 ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac
    15601 ggagacgtgg tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg
    15661 cagctggcac gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc
    15721 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
    15781 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
    15841 ttgcgacgcg gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
    15901 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg
    15961 tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat
    16021 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa
    16081 aaggcttcgc agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa
    16141 tcttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc
    16201 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
    16261 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
    16321 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
    16381 tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc
    16441 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg
    16501 catgaaagtg ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa
    16561 caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa
    16621 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga
    16681 tctgaactat gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
    16741 ggttgaacgt gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc
    16801 tgacgtggta atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt
    16861 cggcattcag aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc
    16921 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
    16981 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc
    17041 tgggcagtat ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa
    17101 aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga
    17161 agctattgcc atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat
    17221 taccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc
    17281 aattttcgcc atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt
    17341 tacgccggtg ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa
    17401 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga
    17461 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
    17521 ttgccggatg cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt
    17581 tcgaatattc tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg
    17641 cttttttatt tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg
    17701 cgagttttca gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc
    17761 tcgacttctt cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc
    17821 atcggtgggc gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc
    17881 ggtacaacgc gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta
    17941 taggtggcaa agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa
    18001 taatatcccc agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc
    18061 gcatgcagac gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg
    18121 ttgccctgat tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca
    18181 ggaatatcga cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc
    18241 atctcttcga tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg
    18301 cgtattgcca actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca
    18361 acaatcgctt cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac
    18421 gcacgacggc gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg
    18481 ggatcacgga actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat
    18541 gctccccagt agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta
    18601 ttttttttcg tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag
    18661 tgtagcggca caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc
    18721 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca
    18781 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
    18841 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc
    18901 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca
    18961 gaatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
    19021 aataacctgc tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca
    19081 ctggcgtacc ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca
    19141 ccggattagt tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga
    19201 atcgcaagaa tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag
    19261 cgcggattgc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
    19321 gctcacgttt accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt
    19381 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
    19441 tggttaaacc ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca
    19501 gattgttaac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
    19561 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
    19621 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
    19681 cggcagcagc aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
    19741 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
    19801 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
    19861 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
    19921 gcgtggcatc acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
    19981 tgagcgaccc caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
    20041 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
    20101 actggttggc taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg
    20161 tccccagggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
    20221 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
    20281 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
    20341 caagcggtgt cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat
    20401 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt
    20461 gtgcctcaaa atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca
    20521 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
    20581 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat
    20641 catcatcgcc gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg
    20701 ttacctgctt cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg
    20761 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
    20821 aataatcatg ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag
    20881 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
    20941 cctgtgttgc acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat
    21001 gtaacataat gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca
    21061 tgactactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
    21121 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat
    21181 tttgacttta cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg
    21241 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
    21301 accgcagaag ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt
    21361 acgccctggt acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
    21421 cctgattaac gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc
    21481 gcgagcattg ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga
    21541 tgtaaatggc caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg
    21601 tggcgtttta atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct
    21661 gtgcctgaat caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
    21721 gtttatttca atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
    21781 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
    21841 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
    21901 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
    21961 tcattacttg gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
    22021 gccgttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
    22081 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt
    22141 agtctgatgt ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca
    22201 gtgacgccag aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg
    22261 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
    22321 ggtgtgaaat tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
    22381 gtagcgatga aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct
    22441 actgcgcgtc gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg
    22501 atggtggcag taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg
    22561 tcggtggtgc tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc
    22621 taatctatcg ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
    22681 cgctatctct ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg
    22741 tcggatgcgg cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag
    22801 tgattccacg cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc
    22861 tgaatcaaat aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc
    22921 gccaggttat ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat
    22981 ttgcggtcca tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca
    23041 gcgatatctg ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc
    23101 agcagacggt tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg
    23161 tcatcactca tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc
    23221 ggcacctgat aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc
    23281 agcgaacctg cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg
    23341 gagcgtgccg ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg
    23401 tacagcactt cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca
    23461 tgcggttcaa ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca
    23521 gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta
    23581 cccaaccccg gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct
    23641 gctttgatga aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt
    23701 aatttagggc gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc
    23761 agacggtctg cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta
    23821 atgtttcact gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg
    23881 gtttatagcc tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg
    23941 ggctagcagg agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac
    24001 ctttaaatcg gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg
    24061 gcagtttcac cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg
    24121 acattccgga gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact
    24181 ctttgaacaa cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac
    24241 gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac
    24301 aggtcatcgg catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg
    24361 gttgtttttc aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga
    24421 atcaaagata atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga
    24481 aaacttgcga ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt
    24541 atattggaag caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat
    24601 acattgcgac tcctgaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    24901 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    24961 ggttccctcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca
    25021 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    25081 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    25141 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    25201 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact
    25261 gaccgaggcg gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc
    25321 atctgcatcg cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg
    25381 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    25441 cttttttcgg cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    25501 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    25561 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    25621 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    25681 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg
    25741 cggatgacgc cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc
    25801 acgcgtctcc gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga
    25861 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    25921 acgcatcagt ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    25981 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    26041 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc
    26101 acctgcttcc agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac
    26161 gccttttttg ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt
    26221 gctgaatgcg tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    26281 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    26341 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    26401 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    26461 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    26521 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    26581 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    26641 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    26701 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    26761 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    26821 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt
    26881 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    26941 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    27001 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc
    27061 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    27121 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    27181 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    27241 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    27301 ggatattgtc ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    27361 cgtgcggcgg agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt
    27421 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    27481 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    27541 cattttccgg tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat
    27601 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    27661 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca
    27721 gtgcgcccat cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    27781 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
    27841 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    27901 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    27961 gcgatgcgcc acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgccggcc
    28021 ccgcaaatac tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg
    28081 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    28141 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    28201 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    28261 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    28321 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    28381 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    28441 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    28501 gcaccaggct ggtgatttta gcgcgggttt tcaggcgctg cgccatttcc gggcgacgca
    28561 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    28621 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
    28681 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
    28741 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    28801 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    28861 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    28921 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    28981 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    29041 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    29101 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
    29161 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    29221 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
    29281 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    29341 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    29401 gcccgtgttt ctggtgcgag ttggctggtc tgccgattac gccgaagcat taaaacagcc
    29461 ggttgatg
//
LOCUS       DAJTOW010000044        29042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTOW010000044 DAJTOW010000000
VERSION     DAJTOW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.044"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO244_002061"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO244_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5663885.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO244_002062"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO244_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5663886.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO244_002063"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO244_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5663887.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO244_002064"
     CDS             4843..5082
                     /locus_tag="OO244_002064"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663888.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO244_002065"
     CDS             5092..5262
                     /locus_tag="OO244_002065"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5663889.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO244_002066"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO244_002066"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5663890.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO244_002067"
     CDS             6724..6990
                     /locus_tag="OO244_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5663891.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO244_002068"
     CDS             7084..7773
                     /locus_tag="OO244_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5663892.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO244_002069"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO244_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5663893.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO244_002070"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO244_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5663894.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO244_002071"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO244_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5663895.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO244_002072"
     CDS             complement(10475..12193)
                     /locus_tag="OO244_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5663896.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO244_002073"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO244_002073"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5663897.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO244_002074"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO244_002074"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5663898.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO244_002075"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO244_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5663899.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO244_002076"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO244_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5663900.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO244_002077"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO244_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO244_002078"
     CDS             18432..18746
                     /locus_tag="OO244_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5663901.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO244_002079"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO244_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5663902.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO244_002080"
     CDS             20570..21862
                     /locus_tag="OO244_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5663903.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNAYSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO244_002081"
     CDS             complement(22016..23665)
                     /locus_tag="OO244_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5663904.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO244_002082"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO244_002082"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5663905.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25870..26334)
                     /gene="soxR"
                     /locus_tag="OO244_002083"
     CDS             complement(25870..26334)
                     /gene="soxR"
                     /locus_tag="OO244_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5663906.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26420..26743
                     /gene="soxS"
                     /locus_tag="OO244_002084"
     CDS             26420..26743
                     /gene="soxS"
                     /locus_tag="OO244_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5663907.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26746..28323)
                     /gene="pdeC"
                     /locus_tag="OO244_002085"
                     /pseudo
     CDS             complement(26746..28323)
                     /gene="pdeC"
                     /locus_tag="OO244_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28752..29033
                     /locus_tag="OO244_002086"
     CDS             28752..29033
                     /locus_tag="OO244_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5663908.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7289 a         7777 c         7346 g         6630 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct agagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcaccag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtgggg
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tagcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctag
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactaatgtt
    19561 tggtacggcg ggcttgccac acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catactccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt
    25381 tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga
    25441 tgtgcaagat tgcagcgtta tcgagccgta taccgttctg gattgcgata aaaaatcgtc
    25501 tgagatgaca aaaatattgc ggctaaaaag tatactttta cactggcgaa gaaacgtcgg
    25561 tgacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg
    25621 tgttgacgtc gggggaaacc ctcctgtgtt ccagcgggat agagagaaag acaaagaccg
    25681 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt gtttgcggat aattccaaaa
    25741 gagctcgatc agtcccctcg ccccttcggg gagagggtta gggtgagggg aacaggcagc
    25801 accagtgcaa atatttaccc tcaccccggc cccatagtta acgatgagtc agcttgtcct
    25861 tgtggcgctt tagttttgtt catcttccag caagcgtgcg ccggtacctt cttctcctaa
    25921 gcggtcgccc gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca
    25981 tccgtccagt tcgtcacgca gcgccactaa ggtatgaatg cgccgatcca actcttctcg
    26041 ccattgggac gaaagctgtt tccactcttt cgcacttaac gtatgccctt ctggcaacac
    26101 gccaaacgct tcaccaatgg tcgccagcgg aatgccaata cgctgagcga ttttgataat
    26161 tgcgacatat cgcaacacat cacgtttata tcgccgctga ttgccgctgt tacggatact
    26221 agtaatcaac cctttgcttt catagaaatg cagcgccgat accgccacac cgctgcgttt
    26281 cgccacttcg ccaggggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg
    26341 ctttacctca agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac
    26401 actgaaaaga ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat
    26461 tgacgtgcat attgaccagc cgcttaacat tgatgtcgtc gcaaaaaaat caggctattc
    26521 aaagtggtac ttgcaacgga tgttccgcac ggtgacgcat cagacgcttg gcgattacat
    26581 tcgccaacgc cgcctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt
    26641 tgatatcgca atggacctgg gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg
    26701 gcagtttgat cgcactccca gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt
    26761 taactcccgc gcgggtaatt gctccatcca ttgcataaac acctgcggcg gcatcgcctt
    26821 cgcaaaaaac catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgctc
    26881 ctcagtttcg acgccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat
    26941 gtgttccgca atcaaatgac tggttttgtg ggtggtcagc gtttcaacaa acgatttatc
    27001 gattttcaaa atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc
    27061 aaaatcatca attgccactt cgtaaccagc ctggcggaaa gccagaatta atcggcgtca
    27121 ttttttcgac atcaagaaat gcatgctcag tcacttcaaa tttaatttgc tgcggacgca
    27181 ccgcgtattg ctctgttttc tgattgattc gcgctatcaa ccgtgacgta tgaaaatcgg
    27241 aggccgacag gttaatagaa acatagcgat cggcatgtgt tgccaggtac gcgcccagat
    27301 cgcggaagac attatcaata acataatcag ttatctgttc tatcatcccc tccttttctg
    27361 ccagcggaat aaactctgcc ggattcatta tttgcccctg ctcaccaggc caacgtaaca
    27421 acgcttcagc gccgatacat ttttctgttt tgatatcgat tattggctgg taataaagac
    27481 aaagttgatg tttttcgagg gcgcgttgca atttacgttt gggagataaa tagttttgtc
    27541 gaatacgtag ccagagtaat agcagaacaa gactccccaa aataccggcg ggcaacgcaa
    27601 aaataagatg attataaaaa tgggttataa gacgttgata tgaagtcgca acaatggctg
    27661 caattgggcg ttttgtcaaa taaattgtcg catataaata gccatttctt tgtacggtta
    27721 aatccttcaa atgaatcagc ggagaaaacg ttgctgccga ggcctctttg cttaacgaga
    27781 aaaaggtttt cgtcaccgta tcatacacac cccattgcaa tgtcgggtca tcagacatca
    27841 cttcactcca gaagagaggg ttgataaccg ccacataatt tccccgctgc atataggtca
    27901 ttttatagcc agaaaaaaaa ggcgtatcgc ggtaataata gatagaaacg ttaggttcac
    27961 gcttataatc ggccggtgca atcgtatagc cgtttacagg cgctatcagc gatgagcata
    28021 aaaaatggtt atcacgaatt cattaatata aagatagcca cgaataatat tcaacattcg
    28081 cttttgatgg gctggagtgc aaacttgccc ctgatagcgt tcagctgcat cgctcactaa
    28141 atctgcctgg cgaatgacca gctcggattt atccagtgcc agttgagcaa aggttcggag
    28201 ctgattattc acttctgatt ttgcccaaag gaaggcaatc catagcgaaa gaatgatggg
    28261 aaagagaact aaaaagataa tgcccggcaa cgccagtaat tggtgtcgtg cacgatgact
    28321 cattgctatc aatttcctgc ctgtttatac ccggccattg caaggtgaaa ccctggctac
    28381 cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt gaaattgata
    28441 cctttttaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta attttctaca
    28501 ggaatacaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca cataatttgt
    28561 acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga tatagttcac
    28621 aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca
    28681 gagtgtacca cgcctcccgt gaacaacgcc gcgctgtcca gggcatcggc tcttttcaga
    28741 ggaattgttt gatggctacc ctcaccactg gcgtggttct tcttcgctgg caacttctta
    28801 gtgccgtaat gatgtttctg gccagcacac tcaacatccg ttttcgtcgg tctgattatg
    28861 tcgggcttgc ggtgatcagc agcggtctgg gcgttgtttc cgcctgttgg ttcgcaatgg
    28921 ggttgcttgg catcacaatg gcggacatca ccgctatctg gcacaacatc gagtcggtga
    28981 tgatagaaga gatgaatcag acaccgccac aatggccaat gattttgacc tgatacgtag
    29041 aa
//
LOCUS       DAJTOW010000045        28915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTOW010000045 DAJTOW010000000
VERSION     DAJTOW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.045"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO244_002087"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO244_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5663909.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO244_002088"
     CDS             complement(2138..2584)
                     /locus_tag="OO244_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5663910.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO244_002089"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO244_002089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5663911.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO244_002090"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO244_002090"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5663912.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5371..6009)
                     /locus_tag="OO244_002091"
     CDS             complement(5371..6009)
                     /locus_tag="OO244_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5663913.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQ"
     gene            6182..6595
                     /locus_tag="OO244_002092"
     CDS             6182..6595
                     /locus_tag="OO244_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5663914.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO244_002093"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO244_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5663915.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO244_002094"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO244_002094"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5663916.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO244_002095"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO244_002095"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5663917.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO244_002096"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO244_002096"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5663918.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO244_002097"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO244_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5663919.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO244_002098"
     tRNA            complement(9861..9948)
                     /locus_tag="OO244_002098"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO244_002099"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO244_002099"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5663920.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO244_002100"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO244_002100"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5663921.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELITVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO244_002101"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO244_002101"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5663922.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO244_002102"
     CDS             14005..14592
                     /locus_tag="OO244_002102"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5663923.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO244_002103"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO244_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5663924.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO244_002104"
     CDS             14984..15841
                     /locus_tag="OO244_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5663925.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO244_002105"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO244_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5663926.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO244_002106"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO244_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5663927.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO244_002107"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO244_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5663928.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO244_002108"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO244_002108"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5663929.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO244_002109"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO244_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO244_002110"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO244_002110"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5663930.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO244_002111"
     CDS             complement(22884..24023)
                     /locus_tag="OO244_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5663931.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO244_002112"
     CDS             complement(24069..26165)
                     /locus_tag="OO244_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5663932.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFYRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO244_002113"
     CDS             complement(26165..26911)
                     /locus_tag="OO244_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5663933.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO244_002114"
     CDS             complement(26908..27552)
                     /locus_tag="OO244_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5663934.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO244_002115"
     CDS             complement(27659..27964)
                     /locus_tag="OO244_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663935.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO244_002116"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO244_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5663936.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7320 c         7601 g         6990 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ctattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatcaccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagatg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gtaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggcaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DAJTOW010000046        28811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTOW010000046 DAJTOW010000000
VERSION     DAJTOW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.046"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..601
                     /locus_tag="OO244_002117"
                     /pseudo
     CDS             1..601
                     /locus_tag="OO244_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            598..984
                     /locus_tag="OO244_002118"
     CDS             598..984
                     /locus_tag="OO244_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663937.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1009..3222
                     /gene="tssI"
                     /locus_tag="OO244_002119"
     CDS             1009..3222
                     /gene="tssI"
                     /locus_tag="OO244_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5663938.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHEGRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3245..3675
                     /locus_tag="OO244_002120"
                     /pseudo
     CDS             3245..3675
                     /locus_tag="OO244_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3712..4119
                     /locus_tag="OO244_002121"
     CDS             3712..4119
                     /locus_tag="OO244_002121"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5663939.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4116..6593
                     /locus_tag="OO244_002122"
     CDS             4116..6593
                     /locus_tag="OO244_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5663940.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDRSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDEQGLAVSRTDARGGEVALAHDARGQLRRYTDCSGCATGYE
                     YDEDGNLTAVTDAEGKTVRISYNRQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGS
                     VQSWQYTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQ
                     RPDGLIRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNR
                     LDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTD
                     ITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADN
                     RLSWQRSLPGGSQNPQQAVLPRRRTTA"
     gene            6590..7579
                     /locus_tag="OO244_002123"
     CDS             6590..7579
                     /locus_tag="OO244_002123"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663941.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7679..7882
                     /locus_tag="OO244_002124"
     CDS             7679..7882
                     /locus_tag="OO244_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663942.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7900..8220
                     /locus_tag="OO244_002125"
     CDS             7900..8220
                     /locus_tag="OO244_002125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5663943.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8286..8518)
                     /locus_tag="OO244_002126"
                     /pseudo
     CDS             complement(8286..8518)
                     /locus_tag="OO244_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8628..9398)
                     /gene="yafV"
                     /locus_tag="OO244_002127"
     CDS             complement(8628..9398)
                     /gene="yafV"
                     /locus_tag="OO244_002127"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5663944.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9579..10025
                     /gene="ivy"
                     /locus_tag="OO244_002128"
     CDS             9579..10025
                     /gene="ivy"
                     /locus_tag="OO244_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5663945.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(10068..12512)
                     /gene="fadE"
                     /locus_tag="OO244_002129"
     CDS             complement(10068..12512)
                     /gene="fadE"
                     /locus_tag="OO244_002129"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5663946.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12752..13330
                     /gene="lpcA"
                     /locus_tag="OO244_002130"
     CDS             12752..13330
                     /gene="lpcA"
                     /locus_tag="OO244_002130"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5663947.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13536..14303
                     /locus_tag="OO244_002131"
     CDS             13536..14303
                     /locus_tag="OO244_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5663948.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14274..15014)
                     /locus_tag="OO244_002132"
     CDS             complement(14274..15014)
                     /locus_tag="OO244_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5663949.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15398..15919
                     /locus_tag="OO244_002133"
                     /pseudo
     CDS             15398..15919
                     /locus_tag="OO244_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            16095..16592
                     /gene="rayT"
                     /locus_tag="OO244_002134"
     CDS             16095..16592
                     /gene="rayT"
                     /locus_tag="OO244_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5663950.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16809..18521)
                     /locus_tag="OO244_002135"
                     /pseudo
     CDS             complement(16809..18521)
                     /locus_tag="OO244_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18493..19278
                     /gene="lafU"
                     /locus_tag="OO244_002136"
     CDS             18493..19278
                     /gene="lafU"
                     /locus_tag="OO244_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5663951.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19349..20404
                     /gene="dinB"
                     /locus_tag="OO244_002137"
     CDS             19349..20404
                     /gene="dinB"
                     /locus_tag="OO244_002137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5663952.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20456..20749
                     /gene="yafN"
                     /locus_tag="OO244_002138"
     CDS             20456..20749
                     /gene="yafN"
                     /locus_tag="OO244_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5663953.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20752..21150
                     /gene="yafO"
                     /locus_tag="OO244_002139"
     CDS             20752..21150
                     /gene="yafO"
                     /locus_tag="OO244_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5663954.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21160..21612
                     /locus_tag="OO244_002140"
     CDS             21160..21612
                     /locus_tag="OO244_002140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5663955.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21858..22901
                     /locus_tag="OO244_002141"
     CDS             21858..22901
                     /locus_tag="OO244_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5663956.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            22963..23577
                     /locus_tag="OO244_002142"
                     /pseudo
     CDS             22963..23577
                     /locus_tag="OO244_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23634..25091)
                     /gene="pepD"
                     /locus_tag="OO244_002143"
     CDS             complement(23634..25091)
                     /gene="pepD"
                     /locus_tag="OO244_002143"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5663957.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25353..25811
                     /gene="gpt"
                     /locus_tag="OO244_002144"
     CDS             25353..25811
                     /gene="gpt"
                     /locus_tag="OO244_002144"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5663958.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25903..27147
                     /gene="frsA"
                     /locus_tag="OO244_002145"
     CDS             25903..27147
                     /gene="frsA"
                     /locus_tag="OO244_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5663959.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27205..27606
                     /gene="crl"
                     /locus_tag="OO244_002146"
     CDS             27205..27606
                     /gene="crl"
                     /locus_tag="OO244_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5663960.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27645..28700)
                     /gene="phoE"
                     /locus_tag="OO244_002147"
     CDS             complement(27645..28700)
                     /gene="phoE"
                     /locus_tag="OO244_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5663961.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7100 a         7170 c         7670 g         6871 t
ORIGIN      
        1 gtatttatta aatcaaaata ctggttcgat tacggctgtg ataagatgga agtatgagta
       61 gtataaggat aataccgtta cagatgactg gactatatgg gaaaaagcta tttttatgcg
      121 agcgatatat ttatctgttc agcaggcatg gaatgggaaa ataacttatt ctgtttccgg
      181 tgaatctgag ttcgctaaaa aatttcaggg taaagctttg ccttttgatg taagaataat
      241 ttctgcatct cagaatgaag actggttggt tattgcaaca aaggttttgc ctggtgctga
      301 tttaagaaca tatgttgatt ttaaaaacag tacggttcat gttgattcag cgggcctgga
      361 aaaagtagcg aaatgtatta actgtaataa tacgttgcaa gtaaatatac ctcatgaagc
      421 cggacatgta cttggttact tagatgatga ttatgattct tcgtctccgt atgttggaga
      481 tattagtggg ttgatgaatg tgggaatgga gcttcgggaa cgatacctta aaaacgcaac
      541 tataacgttg aatatcataa tgcctgaaac taaatttact ttattgaatg taaccaaatg
      601 aaatttttct gtattatagt gattttcctt tcaataaatt acatagctat agtgaatgtt
      661 ttcaagtctt ctgcagttgc tcaatccacc aaatattatg aagaaaatga tatacatgat
      721 ggaagtaatg acatcttttt tatgaagaaa tatgggccgc ctgctcatga gttcgtgagg
      781 ccacgatata aatttgttgg tgagtattat atcggcttga atcaaactta tcgcataaca
      841 gatcctgaat ttcaagatgt tcctattaag gaaatgttct ggaatttgca gggggatttg
      901 aatctgacat gttggtttca ttataaagac gggcaatgga gagtgattac ttatattttt
      961 tggccaccag gggctagatt ttaatccggt attaaaggag caaatatcat gagttttgta
     1021 tctacgggta ataaaccagt tggaaacggt ggacccgtca tcaccacacc accaatagct
     1081 ggcgaaagtg gcggtatgag cacaggttca gctgtaaccg atgtatctgg tgccgccgag
     1141 gagatggcag aacaggctgc ggcggacctg tttggtgcgc tgccggaacc gtcaggtctg
     1201 gttaaagccg cagtagctgc tgcgcaggct gccgctgcag cagcagggat ctcggatatg
     1261 gctggtgcag ttcaggatgc agcggcgtcg cttgcagccg gtgctccggg ggcgcataac
     1321 gtcacagtaa gtggtagtgc ggtgccgccg cagatgcttt tgtttgcagg catgaatggt
     1381 tcggaagggc ttgggaatct gttttcttac acagtacagc ttaaaacgct ggacgccctg
     1441 aacctggggt acgtttcccc cgctgctaac ctgccgcttc agctcatggt gggcaaagac
     1501 ctgtgcgtca gtatcgaact ggacggcggc ggaaaacggt atatcagcgg gctggtgacg
     1561 gcggcgaggg tggtcggcca tgagggccgt tcggtaacgt atgaattacg cattgagcca
     1621 tggctgaagc tgctgaccca taccagcgac tacaaagcgt tccagaacaa aaccgtggtg
     1681 gatattctcg atgaagtact ggatgaatat tcctttccgg tggaaaagcg gctggtggaa
     1741 aattatccac cccgcgcctg gcaggtgcag tatggtgaaa ccgacttcga tttcatccag
     1801 cgcctgatgc aggaatgggg gatttactgg tggtttgagc acagcgagaa cagccacacg
     1861 ctggtgctgg tggatgccat caatggtcat aaagcgtgcc ctgactcgcc gctggtggag
     1921 tggcaccagg aagggctgaa actggataag gaatttatcc acaccattac ggccagtgag
     1981 aggctgcgta ctggaaagtg ggtgatggac gattttgatt ttatgaagcc tcgttcgctg
     2041 cttaaaagta ccgtggccag tccgcgtgat accggtcatg ctgaatacga gcattacgaa
     2101 tggcctggtg attacttcac tacgggcgaa ggcgagatgc tgacgcgcat tcgtatggaa
     2161 gcgcagcgca gcccaggcag ccgggcacat ggggccggtc atatccgtac cctcatgacc
     2221 gggtacacct ttacgttgat gaatcacccg acggcagaaa ttaatcagga atatctgctg
     2281 gtacagacca ctctgttttt acgggataat gcgcagcaca gcggtcagga tcagcatttt
     2341 acctatgtca ccacgtttga actgcacccc accagcgaag tttaccgccc acaacgaacc
     2401 ctgagcaaac cccacaccaa aggcccgcag agcgccatcg tcaccggccc ggcggggcag
     2461 gaaatctgga cggataaata tggccgggtg aaggtgcagt ttggctggga ccgttacgga
     2521 aagaatgacg aaaacagttc ctgctggatc cgcgtcagct atccgtgggc gggtaaagga
     2581 ttcggcatga tccagatccc gcgtattggc caggaagtac tggtggattt caaaaacggc
     2641 gatccggatt tgcccatcat cgtggggcgc acctataacc aggacaccat gccgccgtgg
     2701 gggctgccgg gcatggcgtc gcagagtggg atattcagcc actcattgca gggagggccg
     2761 actaacggaa atatgctgcg cttcgatgat aaaacgggtg cggaagaggt gaagttccat
     2821 gcggaaaaag acctcaatac cacggtaaaa aacaatgaaa cccacacggt catggtcgat
     2881 cgcataaaaa ccattgtcaa aaatgaaacc atcagcgtgg gagaagaccg taatgccacc
     2941 atcacaaaaa atgatggtct ttccgtcaaa ctggctcaaa ccatcaatgt cggaacgaca
     3001 tatcgtctgg atgttggcga tcaatttacc cttcgttgcg gcaatgcagc gcttgtatta
     3061 cacaaggatg gctcgattga gttttgcgga aaacaactgt tactgcacac cagcgatgtt
     3121 atgcaactca ttggcagtgg cattgatatg aatccggatg gcggcacagc catgactgcg
     3181 gatgatattg ccccgttacc tacccccgac gtttctgaat aaaaaatata aaaggaattt
     3241 ttctatgacc agcctttacc gtattcagga gggctgcttt gccctgcctg aaacttttct
     3301 tgatcgcacc gtcaatatct tcgtcccatc gggcaatgaa agggcaactc ccagtctgaa
     3361 tattttccgc gatacgctca ggccagatga aaatctgacc acctatattg accgccagat
     3421 agccttgatg aaaaaaaaaa cctaggccag caccgggtac tttcgcgggg aacggcactg
     3481 gcgggaactg gcgaagctgc catgacaggt gaacagattc tcgcaactca tcgttctgga
     3541 aaaacagaag tgtaccagcg tcaggccggg tttatcaccg ggccagcaaa ggtactaatg
     3601 ttgacgctga ccactcagcg tccttttgat gatcacaccg accagctatg gactgcatgg
     3661 ttgactagtt tttagcctgc taaaagctaa aaaaaacagg gaggttttgt catgtatgaa
     3721 gccgcacggg tggatgatcc catctaccac accagcgccc ttgccgggtt tttgataggg
     3781 gccatcatag gtatcgccat tattgccgtc gccagctttg ctttttttac ctgcggcttt
     3841 ttggccgggc tgattttgag ttttctggct gatcagatag cttccggagt cctgcaactg
     3901 ggtgaggcta ttgggcgctc gatacattcc acagtgggaa aaattctgac cggttctgat
     3961 aatgtcagca ccaacagtcg cccggccgcc cgggccgtac tgagtacggt gatatgcgaa
     4021 gaccattccc cggaaatccg cattgcccaa ggttccggca acatctacat caacagccag
     4081 ctgcccgtaa ggatgaccac actgagtgtg atgcagtgat tgaggacggc tcccccaatg
     4141 tctttctcgg cggcggtacg cagacggtac tggaaatcag cccggaaatc cctgactggc
     4201 tgcgtcaggt ggtggatgtg ctgtatgtgg tcgccggaat gcttggcggg ctggctggcg
     4261 cctggcgtca ggcggcaaag atggggtcga aatttggtac aaaatgtgcg gcaaaattta
     4321 ttggtggcga aatggtcggg atggccatct ctgacacagt catgggattg ttcagcaatc
     4381 cggtagatgt caccactggg caaaaaattt tgctgccgga gacggatttt accctgccag
     4441 gacgcctgcc tgtcacctgt tcgcgctttt acgccagcca cctggaaact gaaggattgc
     4501 tgggcagagg atggcggctg aactgggaga taaacctgcg tgaggatgaa acatatatca
     4561 ccttcatcgg tgtacagggt cgggaactga gttatccgaa agagatgctg ataccgggcc
     4621 accagatatt cgacccggaa gagcagtttt acctgagccg tctgcatgac ggatgctatg
     4681 tgctgcatta caccgaccgc agctattacg tgtttgatga atttgatgac cacggcgtcg
     4741 cgccgttact ttttatggag acgccgtacc ggcagcgtat tgctttcggg cgtgagaacg
     4801 gcaggctggt gcgggtggcc tccagcagcg gacaccatct gttattgcac cgcacgatga
     4861 cgcaagcagg ggaacgcctg tcgcatattg aacttttgaa gggcgggcgt cccggtaacc
     4921 tggtggaata tcgttacgac gataacgggc aactgactgg cgtggtgaac cgggctggtg
     4981 tgacggtgcg ccagtttgcc tatgaaaatg gcctgatgac agagcatcgc aacgcgacgg
     5041 gctttacctg tacctaccac tgggaagaga ttgaaggttt cccgcgcgtg gtggaacaca
     5101 cgaccagcga cggcgaggat taccggttcc actatgattt tgctggcggg cagacggtgg
     5161 taaccggcag gccggagcag aagtggcagt ggtggtttga tgaagagacg tatgtgactg
     5221 cgcaccggac gccgggcggc ggaatgtacc gcttcacgta caacgaaaac cattttccgg
     5281 tagcggtgga actccccggc gaacgcaggg taacgctgga atacgacacc ctgagccggg
     5341 tggtgaaaga gacggacccg gcaggccggg tgacacagac gcagtggaac ggcagttttg
     5401 ctgaaatcac gcgccgggcg ctggatgacg accacgtatg gaaggcggat tacaacgaac
     5461 acggccaggt gatacgggag acggacccgg aagggcgcgt cacgcggtac gggtatgatg
     5521 aacaggggct ggcggtcagc cgcacggatg cgcggggcgg cgaggttgcg ctggcgcacg
     5581 atgcgcgcgg tcaactgagg cgttacaccg actgttcagg gtgcgccacg ggttatgaat
     5641 acgatgaaga cggcaacctg acggcggtga cggacgcgga ggggaaaacg gtacgcatca
     5701 gctataaccg tcaggggctg ccggagacgg tctgccaccc cggtaaacag caggaccgtt
     5761 acacctggaa cgcgctgggg ttactgagca gccaccgtcg catcacgggc agtgtgcagt
     5821 cctggcagta tacgcagcgc gggatgctgg cgcgccatac ggatgaagag aaacgggaga
     5881 cgcgctggca gtacaccccg gaagggctgg ttgccagtct gagcaacggc aacggggctc
     5941 agtaccggtt cagctacgac ggcgacggac ggctgacggg agaacagcgc ccggacggcc
     6001 tgataaggat gtttgcgctg aatgcggacg ggtttccggt catcatcccg acgcagggca
     6061 cagagggcgg ggtgcgcaat gaacagcagg agcgtgatgc gctgggccgc ctgctgcgca
     6121 gtgacacgca gcacagtaca cggaccttca gctacaaccg tcttgaccag ataacggaag
     6181 tgacgctcac ccccacggaa gaaggggaac ggctgcacca catgcaggcg gacacggtgc
     6241 gttttgcgta tgaccgcagc gggtggctga cggcagagca cagcgtgcac ggcagtatca
     6301 aataccggcg cgatgcgctg ggcaatccca ccgatatcac gttgccggac gggcagcatc
     6361 tcagtcatct ttactacggc agcgggcatc tgctccagac ggcactggac ggtatcacgg
     6421 tcagtgaata tgagcgtgac agcctgcacc gtcaggtgat acgcacgcag gggaaactgg
     6481 cgacgttcag cggctacaac gccgacaacc ggttaagctg gcagcgcagc ctgccaggtg
     6541 gcagccagaa tccacagcag gcggtactcc cccggcgcag aacgacagcg tgacggcgcg
     6601 ggactatatc tggaacgcag acggtgaagt cggcggcatc aatgacaaac tgcgcggttg
     6661 cctggtgttc agctatgacc gcagcggctg gctgaccagc cgcacggggc agatgtatga
     6721 ccatgaccat tattattacg acaaagcggg caacctgctg acggatgagt atcagggagc
     6781 ggtgatggat aaccgcctgc cgggttacgg gcgtgaccgc taccgctata acgaatgggg
     6841 cgaactgaca gagcgcaggg accagcagct ggagtggaac gcgcagggcc agttaacgcg
     6901 ggtcatcagc agcaacagcg agacgcggta tcagtacgat gcgctgggca ggcgcatcag
     6961 caaggcgacc agcaatctcc ataccgaccg gggagaacga agccggacaa cgtttgtgtg
     7021 ggaagggttc cggctgttac aggagacaac gtggcaggga aagcggacat acctgtacga
     7081 tgcggaacag ccgtacacgc cggtggctgc catcacgggg cgcggagaga gccagaaaat
     7141 ctggtattac cacacggacc tgacgggcac ggtacacgag gtaaccgcgc cggacgggac
     7201 gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac cggggcgaca tcagcaacag
     7261 cggggcgtac tttgagcagc cgcagcggct gccggggcag tactttgacg aagaaacagg
     7321 gctgcattac aatctgttca ggtattatgc gccggagtgc gggaggttcg taagccagga
     7381 cccgatagga ttaaacggtg ggttgaactt atatgcttat gcgcctaatc cgttaggttg
     7441 gatcgatccg ctaggactga attctttagg aatagattca aataatatat ttagaggaga
     7501 tggtaactat aaaggtggcg gaattggaca gccaaagagt ggcacaatat cagcacagga
     7561 cattattgat catctctaag aaaaaggtga tgggcgttta acatctttta gtactaaaaa
     7621 aacatctagt tccaactctc gtggtgcaga ttttttctca aaaaaccatg tcttcaaagt
     7681 ggcagtttct gatctcgatg atttagttaa aaataatcag atcactgttt taacacaaga
     7741 aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa attagaaaag aacttggtaa
     7801 tattcttcag aatatggcta ggaataatga ggtgttagtt catgggcaaa ttcttggaaa
     7861 taaagttagc cgatgtaatt agttagtgga gaggaagttg tggacgaaat tagaataaga
     7921 attgctggta atacggctgc gccttgtttc catgtgctaa aaaataaggg atataaagta
     7981 acaatggaaa ctcatgcatt atctgacgat tgtttagatt gtgtatatga ttggtatgcg
     8041 gaaaaaaatg gacaattttt tagtgcaacg agtccagaag agttacttgg attagttgca
     8101 atgtgggaag ttcgtggtaa tgactggaga gctaatttga atgagataaa tacttatcaa
     8161 aaagccatag aagaatcccc gttttttgat aaaaacggca atgaaataga gtctgactaa
     8221 attttattga tatagcaacg ataataaccg gaaatttctt ccagatcttt ccggcttttt
     8281 attatttaac tttcgttcag cacactaaac atcccgtctc gcatcccctt aactggctgt
     8341 ttaacctgat aaagttccct gcgcagttcc tgtacttcgt tgttatccca agcaggatca
     8401 aacatccctc cgagatcttc acggtaacgc ccgtgccgat ctcaaatccc gcttttctca
     8461 gccatttggc gctgattttg atcaccggcg tgtcgctgcg gttgggcaga taacccacgg
     8521 tacagcgacg ctgtgttttg gaaatggtga cttctggcgt aatattgtgt ttaagttagg
     8581 ggctttcgta atctagccca gcccccggcc ctaaacacaa cccccactca ccacaaccta
     8641 aactcatccg catcccgcca tgccggaaac ttttctctat attcccgcaa tgccaccatc
     8701 gacagctccg catcaatacg cgttgcctta tgcgcgtcgg cggtagcgat aatctcgcct
     8761 tgcggattaa tcacccggct gtcaccgcga taatggcagc cgttgccatc gctgccgacg
     8821 cgattgcatc ccgccacata cgcctgattc tcaatcgcgc gggccgtcag caatgcctgc
     8881 cagtgcagag agcgcggagc tggccagttg gcgacgtaca gggcgaggtc ataatcgttg
     8941 agattgcgcg accacactgg aaaacgtaag tcgtagcaca ccagcggcaa aatacgccag
     9001 ccgcgccatt ccacaatcac tcgcgcattg cccgctttat aatgcagatg ctcatctgcc
     9061 atgcggaaca gatgacgctt atcataaaaa tgtaccgtgc cgcccggctc aaccagcaaa
     9121 aagcggttaa ccgaaccaga ctccgtttgt aatgcaacac tgcctgcaat cagcgcattg
     9181 cactgctgcg ccttagctgt catccagttc actacgtcat cttgtgcaag cgacgaagct
     9241 gccgcttcca tggcaaagcc gctggtaaac atctccggta gaacgatcac atcgcgcccg
     9301 gtaatacctt ccagttgacg atcaaaatgg cgcaggttgg caggaccatc cacccacacc
     9361 aacggttgct gcaaaagcgt aatcttcaaa ctaggcacgg tgtacaactc ctttatgcga
     9421 agggttttat aactttaaca ccttatcagg cagttgcctt agcgcagaat aaattgataa
     9481 caaatgctga tattggaaat atctgatttg caaattatcg tgttatcgcc aggctttagg
     9541 aggttaataa catgggcagg ataagctcgg gaggaatgat gtttaaggca ataacgacag
     9601 tcgccgcact ggtcatcgcc accagtgcaa tggcgcagga tgatttaacc attagcagcc
     9661 ttgcaaaggg cgaaaccacc aaagctgcgt ttaatcagat ggtgcaaggg cataagctac
     9721 ctgcatgggt gatgaaaggc ggtacttata ctcccgcaca aaccgtaacg ttgggagatg
     9781 agacgtatca ggtgatgagc gcgtgcaaac cgcatgactg tggctcgcaa cgtatcgctg
     9841 tgatgtggtc cgagaaatct aatcagatga cggggctgtt ctcggctatt gatgagaaaa
     9901 cgtcgcaaga gaaactcacc tggctgaatg tgaacgatgc gctttcgatt gatggtaaaa
     9961 cggtgctgtt cgcggcgttg accggcagcc tggaaaacca tccggatggc tttaatttta
    10021 aataattagc ggataaagaa acggagcctt tcggctccgt tattcaatta cgcggcttca
    10081 actttccgca ctttctccgg caactttacc ggcttcgtcg ccagctcttc cggatcaaag
    10141 tcatcaacgt taatactgcg cagacggctt tcttccgctt tcaccagaat agcggcttca
    10201 tctttatcaa tcagcccctt cgtcagcgcg ttgtgcgcca gttcatccag acgggtaaat
    10261 ggcagatttt tacccagctc tttacagatc cgctgatgaa tcgggtcggc ggcaatcaca
    10321 tccaccaacg cctcttccag taaaccaacc gggttatgct cgcttggcgt caggtactga
    10381 ccgcgaccaa tacgtgaacg agtggcgttc ggcacttgta aaatcttcgc cattttatga
    10441 tccagcttgt cagacggtgc cagataatga cgtccggtcg ggaagatcac cacattcagc
    10501 agcccggcaa ccacgcggtt cgggaagttt tgcagtaaat tgtccattgc ctgttctgcc
    10561 tgatacagcg catcctgtac gccccagtgc accagcggca gatcggcttc attacggcct
    10621 tcgtcgtcat aacgcttcag aacggcagag gcgaggtaaa gctggcttaa aatatccccc
    10681 agacgtgccg agatacgctc acgacgtttc aggctgccgc ccagcagtgc catcgagaca
    10741 tcagaaagca gggcgaggtt ggcgctcagg cggttcaggt gctgatagta gcgtttagtg
    10801 gcatcgccgg ttggcgtgct gctggttaaa ccgcgcgtca ggcccagcca gaagctgcga
    10861 actttgttgc taccgacgtg accgatatgt ttgaacaaca gtttatcgaa cgcgttgacg
    10921 tcattgttct tcgccgcttc catctcttcc agcacgtacg gatggcaacg aatcgctcct
    10981 tgtccgaaga tcatcatgct gcgggtcaga atgttagccc cttcaacggt gatggcaatc
    11041 ggtgcgccct ggtaagcacg cgccaggaag ttgctttgcc cgagcataat gcctttaccg
    11101 ccggtaatat ccatcgcatc aataatcgac tgctgcccgc ggtgggtaca gtgatactta
    11161 acgatagccg acagcacggc aggtttttcg ccgagcataa tgccgtaggt aatcagcgat
    11221 gccgcagcat ccattacata agcgttcccg gcaatacgcg ccagcggctc ttcaatccct
    11281 tccatcttac cgatagagat tttgaactga cggcgaatgt gagaatacgc gcccgttgcc
    11341 agcgctaccg atttcacgcc gccggttgag ttggaaggca gggtgatgcc gcggcctacc
    11401 gagaggcact ccaccagcat ccgccagcct tgcccggcca ttttcggccc gccgatgatg
    11461 taatcgatcg gcacgaagac atctttaccg cgcgtcggtc cgttctggaa cggtacgttc
    11521 agcgggaagt ggcgacgacc aatttccacg cccggcgtgg tggttgggat cagcgcacag
    11581 gtaatgccta aatcttctgc accgccgagt aatttttccg ggtcggagag tttaaacgcc
    11641 agcccaagca cggtcgcaat cggtgccagc gtaatgtagc gtttgttcca ggtcagacgc
    11701 atccccagca cctgctggcc ctgccattcg cccatgcaga caatcccggt gtccggaatc
    11761 gcgcccgcat cggaacccgc ttccgggctg gtcagtgcaa agcaggggat ctcctgacca
    11821 cgcgccagac gcggcagata gtgatctttc tgctcgtcag tgccgtaatg ttgcaacagt
    11881 tcgcccgggc ctaatgagtt tggcacgccg acggtaatcg ccaggatccc gctcacgcca
    11941 gagagttttt gcagcacacg agactgggca taagctgaga actccagtcc gccgtactct
    12001 tttttgataa tcatcgcgaa gaagcgatgc tctttaaggt acgcccacaa ctccggcggc
    12061 agatccgcca gctcatgagt aatctggaaa tcattcgcca tccggcaggc ttcttctacc
    12121 gggccgtcga gaaacgcttg ctcttcagcg gtcaggcgcg gctgcggata gttatgcagc
    12181 tttttccagt ccggcttgcc ctggaacaag tcgccctccc accaggtggt gcccgcatca
    12241 atcgcttctt tctcagtgcg cgacatcggc ggcatcacct tacggaaacc gcgaaatacc
    12301 ggcgcggaaa tcatcgactt acgcataggc gcaaagttaa atggcacgag gataatggtc
    12361 agaggcacca gtacccacgc cgaccacaga ccagcaacgc cgagggcggc tgtccaggcg
    12421 agcaaaatca gactgctgat aaataagctc acgcggtgat agaacaacgc gccgagcagg
    12481 acaaccgtag cgagaatact caaaatcatc ataacgaaaa gccccttact tgtaggaggt
    12541 ctgaccactt gtgatgatat ggttgtagtg gatgtaaaaa catttagcaa tgtgtttaca
    12601 atataattac aacaaagctc acattgttgc tgtttttatc cgcacttcag gtcaaaaagt
    12661 cctggtcata gcacctgccc gtacttctcg cttttggcgg tatccggtac actgcatttt
    12721 gtctattaca tttatgctga aggatatcct catgtaccag gatcttattc gtaacgaact
    12781 gaacgaagcg gcggaaacgc tggctaactt tttaaaagat gacgccaata ttcacgccat
    12841 tcagcgcgcg gcggtcctgt tagcagacag ctttaaagcc ggtggcaaag tgctttcctg
    12901 cggcaacggc ggttcccatt gcgacgctat gcactttgcc gaagagttga ccggtcgcta
    12961 ccgtgaaaac cgtccgggct acccggcgat tgctatttct gacgttagtc atatttcctg
    13021 cgtcggtaat gatttcggtt tcaatgatat tttctcccgc tacgttgaag cggtaggtcg
    13081 cgaaggcgat gtactgctgg ggatctccac ctccggtaac tctgcaaacg tgatcaaagc
    13141 gatcgcagcg gcgcgtgaga aggggatgaa agtgatcacc ctgaccggta aagacggcgg
    13201 taaaatggct ggcacggcgg atatcgaaat tcgcgttccg cactttggtt atgccgaccg
    13261 cattcaggag attcacatta aagtgatcca tatcctgatc cagttaattg aaaaagagat
    13321 ggttaagtaa gtctggcgta ggccggataa ggcgtttacg ccgcatccgg cacttgtgcg
    13381 ctgatgcctg atgcgatgct gacgcgtctt atcatgccta caaatctgta cccgaaccgt
    13441 aggccgaata attcgtttac gccgcatccg acctgaaaat tcttaaatca atcttcgccg
    13501 gggtccatgc gctcccgctg ttgtggaggt tacccatgtg cgaattgctc gggatgagcg
    13561 ccaacgtccc taccgatatc tgctttagtt tcaccggact tgtacagcgt ggtggtggaa
    13621 ccgggccaca taaagatggc tggggcatta ccttttacga aggtaaaggc tgtcgcacat
    13681 ttaaagatcc acaacccagc tttaattccc ccatcgccaa acttgtccag gactacccga
    13741 taaaatcctg ttcggtggtg gctcatattc gccaggctaa tcggggcgag gtggcgctgg
    13801 aaaatactca cccatttacc cgcgagttat gggggcgtaa ctggacttat gcccataacg
    13861 gacaactgac gggctacaaa tcactggaaa ccggcaactt ccgcccggtc ggtgaaaccg
    13921 acagcgaaaa agccttttgc tggctgctgc ataaattaac gcagcgttac ccgcgcacgc
    13981 cgggcaacat ggcggcagtg tttaaatata tcgcctcact ggcggatgaa ctgcgggcga
    14041 agggcgtttt caacatgctg ctttcggacg ggcgctatgt aatggcgtat tgctcgacta
    14101 atttacactg gatcacccgc cgcgcgccgt ttggcgtggc aacgttgctg gatcaggatg
    14161 tggaaatcga cttcagctcg cagaccacac cgaatgatgt ggtcacggtg attgcgacac
    14221 agccgctgac gggcaatgaa acctggcaaa aaattatgcc aggcgaatgg cgcttatttt
    14281 gcctcgggga gcgtgtagtt tgatgccagt tgtggctgca ccacttcgtg gcttaacggc
    14341 ttgctgacca cgtaacggcc attggccaca gaaacggtcg gtggcttacg ggtttgctca
    14401 aagtagtcgt agcccggctt cagctgctcc cagaagtcct taaagttgga atatttatgg
    14461 cgcttcatat tggcgtcggt catgcggaat gggtaaatac tcacctgcac gctcggctga
    14521 ccaaacacca gcgcaccagt aacgaactgg aagatctcat caataccctg attggtcatt
    14581 gcgtagcagc cgatggatac acaatcgccg tggatcatca ggtatttccc ttcataacca
    14641 tgcgcacggt cataagcatt ggggaatcca atattaatcg ctttgtagta acggctgtct
    14701 ggttttaact gattacgctg gacgctataa aacccttccg ggcttttgaa atcgccctga
    14761 cgctgttttg gccctaagcc gccggaatat ttacagattt tataactgtc gagcagttga
    14821 tattgctcgc ccattttgac gtaaagatcg agcgtacgtt cttccttgaa gatctggatg
    14881 tagacagggg atcccatcaa ctgctgctta tactctttgc tcactggcgt ggtcgagcta
    14941 ctgctgccca gcaaaccggc aaacgaaacg cacgggatca acaacatcgc aagaattaat
    15001 gcgattttac gcatactgct tattccttga taaaacggtt acacacgcca ggacggcaaa
    15061 atggatccca aatcggaata gtctggattt ggaaggctca cattatcacc aaaagagttt
    15121 tacgcaagcc tgtcggcgct gggtttacaa atttagcaca ggggcagttt tcaatagtcc
    15181 gaaaagcgca tgtatcattt atttcctaat attatgaaat ctcaatagta atatgcaaat
    15241 aagcaattaa taatcccgtt tactcctccg gtcttttaaa attctcacct tcattcagcc
    15301 ggtttatgtc tttcatgtct tcgttcttgc tcggcagatt tttgcatcca ggcgtgtttt
    15361 cgttatgcgt tttgctccct ttactcgcca gcgccaccac aaccgggagc agataaaaca
    15421 gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac agacgctggc aggcgctggc
    15481 ggaaaacaac cactttttaa gcagcgatca cctgcataac atcaccgaag tggcgattca
    15541 ccgcctggag cagcagcttg gcaagcctta cgtctggggc ggcacgcggc ccgatcaagg
    15601 cttcgattgt agtgggttag ttttttatgc ctacaacaag atccttgagg cgaaactccc
    15661 gcgcacggcc aatgagatgt accactaccg tcgggcaacg attgtggcga ataacgacct
    15721 gcgccgggga gatttgctat ttttccatat ccacagccgc gagattgccg accatatggg
    15781 ggtgtatctg ggcgacggtc agtttattga gtcactgcgc accggggaaa ccattcgggt
    15841 aagccggtta gccgaacctt tctggcagga ccatttttta ggcgcgcgca ggattttgac
    15901 ggaagagatg attttgtagg ccggataagg cgtttacgcc gcatccggcg gttgtgcacg
    15961 gatgcctgat gcgacgctga cgcgtcttat catgcctaca aatctgtgcg tttttgcgag
    16021 ccgctttccc gatataacac agcgtcattg tcggcctttc cttttcctct taccgttgag
    16081 aaaaaggagt cgctatgtcg gaatatcgtc gttattacat caagggggga acatggtttt
    16141 tcacggtaaa tttacgcaat cgtcgaagcc atcttctgac cacccagttc cagacgctcc
    16201 gtaacgccat tattaacgtt aagcgagaca gaccttttga aattaacgcc tgggtcgttt
    16261 tgccggagca tatgcattgc atatggacat tacccgaaag cgatgatgat ttttcctctc
    16321 gctggcggga gattaaaaag cagttcaccc atgcttgcgg attgaaaaat atctggcaac
    16381 cgcgtttttg ggagcatgcc attcgcaata caaaagatta tcgccatcat gttgattata
    16441 tttatataaa tccagagaaa catggctggg taaagcaggt gagtgattgg ccattctcaa
    16501 cgttccatcg cgatgtcgcg cgagggttat atcccatcga ttgggcggga gacataacgg
    16561 atttaagtgc cggggagcgt atcattttat aactgtgcgc tggtgccgga tgcgcttacg
    16621 cttatccggc ctacaaatcg tgctttaacg gtaggccgga taaggcgttc acgccgcatc
    16681 cggcagttat gcgcagatgc ctgatgcgac gctggcgcgt cttatcatgc ctaaaaactt
    16741 gtgcctgaac ggtaggccgg ataaggcgtt tacgccgcat ccggcaattg tgcaccgatg
    16801 ccggattctt acatcaacgc ccccataatc ttcaactcca gctcatccgg cacttcgtta
    16861 tacgacagca catgcagccc cggcgcaaac aaccttgcat aacgcggcag caaagggcgt
    16921 agctgcggcg gcaccagcag caccggatct ttccccgccg ctttcatctg ctctttcacc
    16981 tgtggcatcg tgctctggaa ctggttgagc atattcgggt caaccggcac gctgtcgagc
    17041 atcactttgc cggcctgttg cgcctgattc actacgttgg tcagcagatt ttccagctca
    17101 ttattcagcg tatacaccgt cagctcctgc ttgcgaacga acggatgagt aatgctgcgc
    17161 cgcagcgcca ggcgcacatc ggccgccagc agaatatgat ctttagtcac cgtgctactg
    17221 gcgaccagca cggtggcgat ggtgacgata tcgcgcaggg aaacgccttc ggtcagcagc
    17281 gcccggtaca ctttcagcaa ttggctgtaa ttgagcgccg cgcttaaatc ctccgccagg
    17341 cgcggtgcca tcgacgacaa acggttatgc aactgcgtga tgtcatcata gttaaacaaa
    17401 tcaggaatat agctgcgcac aatcttgttc acatgggtgg caatcacact ggcgctgtcg
    17461 atcacctgat accccatatt cagagccttc gctttctgcg caggctggat ccaggtgacc
    17521 ggtatcccgt acgccgggtc gttccccagc acgccgtcaa tctcgccgta ggtttcgctg
    17581 gagggcagcg ccatcagttt atccgccgga atatccgctt catcagcctc aatgccgttg
    17641 atgaaaatag cgtactggct gggcttgagg cggaagtttt cccgaatgcg gatctccggc
    17701 agcagcacgc cgttaccgtc ggaaatcacc tgccgaacgc cgcgaatccg ctgggtgagc
    17761 gggttgccct gcgctttgtc caccagcgcc accagcttgt agccgaggct taagctgatg
    17821 ggctcgatca gcggaatagt ttcccaactg acctgttgtt cgctggtttc agtgatggtg
    17881 cgggtcagcg tttcgaggct tttctcttcc gcctccgccg cctgcgggcg tttgctcatc
    17941 cgccagccgg taaagccaag cagggcgctg aacagcaaaa acggcagatg cggcatcccc
    18001 ggcaccaccg ccagcacgaa catgatcccg gtggcggtgt agagcaccga cgggcttgcc
    18061 agcaactggt ggcgcacgtc atgggcgata tcgccgttgt cgctgacgcg ggtgacgata
    18121 atcgccgccg cggtggagag cagcagggaa gggatctgcg ccaccaggcc gtcgccgatg
    18181 gtcatcagca cgtattgctg gaaggcggcg tcggcgctca ggttgtattt gaagatgccg
    18241 atacagacgc cgccgctcag gttgatcgcc agaatcatca tcccggcgat ggcgtctccg
    18301 cgtacaaact tcgatgcccc gtccatcgcg ccgtagaaat cggcctcgct ggcaacatct
    18361 ttacgccggg tttgcgcctg cgcctggttg atcaatccgg cgttaagatc ggcgtcaatc
    18421 gccatctgtt tgccgggcat cgcgtccagg gtaaagcggg cagaaacctc ggaaatacgc
    18481 tcggcctctt tggtgacgac gataaagttg attgtcaaca gcgtcagtaa atccgagcgt
    18541 gaaagcatta tcgccgcgct gcacgggcag tcgattttta acggtggtgg gctgtcgccg
    18601 ttaaataaaa tcagcccgtc tcatccgcca aaacctgcca ccgtggcggt gccggaagag
    18661 acggagaaaa aagcgcgtga tgtgaatgag aaaacggcgc tgctgaagaa gaaatccgcc
    18721 accgagcttg gcgagttggc aaccagtatc aacaccattg cccgtgatgc gcatatggaa
    18781 gcgaatctgg agatggagat tgttccccag ggattacgcg tgctgattaa agacgaccag
    18841 aatcgcaata tgttcgaacg cggcagcgcg cagattatgc cgttctttaa aactttgctg
    18901 gtggagctcg cgccagtgtt cgactcgctc tataacaaaa ttattattac cgggcatacc
    18961 gatgcgatgg cctacaaaaa caatatctac aacaactgga acctttcggg cgatcgcgcg
    19021 ctttcagctc gtcgggtgct ggaagaggcc ggaatgccgg aagataaagt gatgcaggta
    19081 agcgcaatgg cggaccagat gctgctggac gcgaagaatc cgcaaagcgc gggcaatcgg
    19141 cgtattgaga ttatggtgct gaccaaaagt gcgtccgata cgctgtatca atactttggt
    19201 cagcatgggg ataaagtggt gcagccgctg gtgcaaaagc tggataagca gcaggtgctt
    19261 tcgcagcgaa tgcgctaaat gctgaatctt tacgcatttc tcaaaacctg aaatcactgt
    19321 atactttacc agtgttgaga ggtgagcaat gcgtaaaatc attcatgtgg atatggactg
    19381 ctttttcgca gcggtggaga tgcgcgacaa tcccgctctg cgcgatatcc ctattgctat
    19441 tggcggcagc cgcgaacgtc ggggggtgat cagcaccgcc aattatcccg cgcgtaaatt
    19501 tggcgtacgt agcgctatgc ctactgggat ggcgctcaaa ttatgcccac atctcacctt
    19561 gcttccgggg cgctttgacg cctacaaaga agcctcaaat catatccgtg aaatcttctc
    19621 gcgctacacc tcgcgcattg aaccgttgtc actggatgag gcttatctcg atgtcaccga
    19681 tagcgtccat tgccacggtt ctgcgaccct catcgcccag gaaatccgcc agacgatctt
    19741 caacgagctg caactgacgg catctgcggg cgtggcacca gtaaagtttc tcgccaaaat
    19801 cgcctccgac atgaataaac ccaacggtca gtttgtgatt acgccggcag aagttccggc
    19861 atttttacaa accttaccac tggaaaaaat ccccggcgtc ggcaaagtct cggcggcaaa
    19921 actggaagcg atggggctgc ggacctgcgg tgatgtacaa aagtgtgatc tggtgattct
    19981 gcttaaacgc tttggcaaat ttggccgcat tttgtgggag cgtagtcagg ggattgacga
    20041 gcgcgacgtt aacagcgaac ggttacgaaa atccgtcggc gtggaacgca cgatggcgga
    20101 agatattcat cactggtctg aatgtgaagc gattatcgag cggctgtatc cggaacttga
    20161 acgccgtctg gcaaaggtaa aacctgattt actgattgct cgccaggggg tgaaattaaa
    20221 gttcgacgat tttcagcaga ccacccagga gcacgtctgg ccgcggctga ataaagctga
    20281 cttaatcgcc accgcgcgta aaacctggga tgaacgccgc ggcgggcgcg gtgtgcgtct
    20341 ggtggggctg catgtgacgt tgcttgaccc gcaaatggaa agacaactgg tgctgggatt
    20401 atgatgtata ctattatgta tattctggtg tgcattatta tgagggtatc actgtatgca
    20461 tcgaattctc gctgaaaaat cggtcaatat cactgagtta cgtaaaaacc cagctaaata
    20521 ctttattgat caaccagttg cggttctttc taataatcgc cccgcaggat atctcttaag
    20581 tgccagcgca ttcgaagcgt taatggacat gcttgctgaa caagaggaga aaaagcccat
    20641 aaaggcgcgc tttcgtccaa gtgctgcaag attagaggaa attacacgcc gcgctaaaca
    20701 atatcttaat gatatgacgg atgatgattt caatgacttt aaggaataag gatgcgggta
    20761 ttcaaaacaa aacttattcg cctgcaactt acagcagagg aacttgatgc gttaacggcg
    20821 gattttattt cctataagcg tgacggtgtt ttgccagata tatttggtcg cgatgcactc
    20881 tacgacgact cgtttacctg gccattaatc aaatttgagc gagttgctca tattcatctg
    20941 gcaaatgtga ataatccatt tccgccacag ttgcgccaat tcagcagaac gaatgacgaa
    21001 gcgcatttgg tatattgtca gggtgcgttt gatgagcaag catggttgct cattgccatt
    21061 ctgaagcctg aacctcataa actggctcga gataacaacc aaatgcataa aattggaaaa
    21121 atggcagaag cgtttcgcat gcgtttttga atttatatta tgaataacat acaaataaga
    21181 gattatcagc ctggcgattt tcagcaacta tgcgctattt tcattagagc ggttacgatg
    21241 accgtcagtc agcattattc actacaacaa attgccgcct gggcgcagat tgacgaatct
    21301 cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagttattaa tgcaaaactg
    21361 gtcggcttta ttacctgcgt tgaacactat atcgatatgt tatttgttga ccctgaatac
    21421 acccgccgtg gggttgccag cgctttgtta aaacctttta ttaagtctga atccgaactt
    21481 acggtggacg caagcataac cgcaaaaccc ttttttgaac gttatggttt tcagacagtt
    21541 aagcagcagc gcgttgaatg ccggggagcg tggtttatta atttttatat gcgttataaa
    21601 ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg cctagtatgt gcgcgttttc
    21661 ttattccaca aactgcaagg aggtaaatca tgacaaatcc tttatcaatg actctttgca
    21721 gacctttcca ggattaattc tttttttctt gccctggatt cgtctgccat ttcctgattt
    21781 tttaattgtt actctcaata attcaagtcg cagcacatgc aacttgaagt atgacgagtc
    21841 tatttacgga atggtatatg ggcaattata ttcgtccctt atccgatgcg gtatttacca
    21901 tcgcatctga tgacctgtgg atcgagagtt tagcgatcca acaattacac accacggcaa
    21961 atttacccaa catgcagcgc gtagtgggga tgccagattt acaccccgga cgcggctacc
    22021 cgattggcgc agcgttcttc tccgcaggcc gtttttaccc agcactggtc ggcaatgata
    22081 tcggctgtgg catggcgcta tggcaaacag atattctcgc tcgcaaatac aacgccgata
    22141 agtttgaaaa gcgattatca gctttggatg acgttgctga agaaagctgg ctggaggaaa
    22201 acctgccgtc agcatttgca cagcatccgt ggcgcagctc gcttggctct atcggtggtg
    22261 gcaatcattt cgctgaactg caacaagttg atcaaatcat caacgctgaa ctgtttgcac
    22321 tggcaggtct ggatgcgcag catctgcaac tgctggttca tagcggctcg agggggctag
    22381 ggcagtctat tttacagcgg catattgcct cgttttcgca tcatggtttg cctgaaggca
    22441 gtgacgacgc gctggcgttt gcgcgtttta atcgccacct gatcgctttg cgcataatgc
    22501 aacaggttaa ggccaccgga agtccggttc tggatgtggc gcataacttt gttagcgcgt
    22561 gtcgaatcgg tgatcaacaa ggggtcttac atcgtaaagg tgccacgcct gatgactgtg
    22621 gtctggtggt tattcccggt tcacgcggtg attactccag gctggttcag cccgtcagga
    22681 gtgaggaaac attgcattcg ctggcgcatg gggctgggcg taaatggggg cgcaccgagt
    22741 gtaaagggcg tctggcagcg aaatacacag cgacgcaact ctcgcgtact gaacttggca
    22801 gtcgggtaat ttgtcgcgat aaacaactca tcttcgaaga agcgccacaa gcctataaat
    22861 cggctgaaag tgtggtgcaa tgtctgggca ggccggatta attattcctg tcgcgcggct
    22921 gcgtccggtg ctaacgctca aaaacagtgg agggaaaaaa ggatgatctt gctataactc
    22981 tcctcagcgc aggggccgga agaatgttgt ctcgcagtga aaaaggcgct ggacaggcta
    23041 attaaagaag ctgcccgaca agacgtcgcg gtaacggtgc tggaaacaga aacaggccgc
    23101 tactctgaca cgctgcgttc ggcgctgatt tctctggatg gcgacaacgc atgggcgtta
    23161 agcgaaagct ggtgtggcac tattcagtgg atttgcccga gcccgtatcg cccacatcac
    23221 gggcgtaaaa actggtttct gggcattggg cgttttaccg ctgatgagca ggagcaatcc
    23281 gatgcgatcc ggtatgagac gctgcgttcg tcggggccgg gcggtcaaca tgtcaataaa
    23341 accgactcgg cggtacgcgc cacgcatttg gcatccggta ttagcgtgaa ggttcagtca
    23401 gagcgcagcc agcatgctaa caaacggctg gcgcgattgc tgattgcctg gaagctggat
    23461 cagcagcaat aggaaaatag cgcggtgctg aaatcacagc ggcgaatgtt ccatcaccag
    23521 attgaacgtg gcaacccgcg acgaacgttt acagggatgg cttttatcga aggataatga
    23581 aggatgaaac tgccggaagg cgattaaacg ccatccggca gcaaatcaaa taattacttc
    23641 gccggaattt ctttcagcag ttcagtcagc agtgtccagt aatgacctac gctttcgatg
    23701 tgaacttgct catccggaga gtgtggaccg gtgatagttg gcccgataga aaccatgtcc
    23761 atttccggat acggtttttt gaacagacca cattccaggc ccgcgtggat aatctggatg
    23821 ttcggcgtct tgttgaacag gcgctgatag gtttcacgta ccagatgcat caccggagaa
    23881 ttagcgtccg gctgccagcc aggatatgcg cctttcgctt cggttttcgc gccagccagt
    23941 ttacccagcg aatccagcat gctcaccacg tagtctttac cgctgtcgat tagtgaacgg
    24001 atcaggcagt gaatttctac gttattgtca gtcatggtca ccacaccgac gttcagggag
    24061 gtttcgacca caccttttgc cacgtcggaa ttgcggatca caccgttcgg ggtggcgttc
    24121 agcagacgaa taaaggtatc gcgagatttc gcaatcaggg cagctttatc gttcgctaca
    24181 gagtccagca acaaggccag atttttctct ttctctgcca gctcgttttt caggatctcc
    24241 tgataggtat tcaccagaga tttcagtgcg tcgactttat cagctgcgac agcaatggtc
    24301 gcaaaggctt cacgcgggat ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc
    24361 aggtccagtt cttctgcatg acccgccagg aagcgcacca gcagtttgtt ggcattaccc
    24421 aggccaacgt ggatttcacc gccggagtga ccgcctttca gaccttttaa ggttaacttg
    24481 aaggtttcaa aaccagctgg aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg
    24541 ataccccccg cacaacccat gtagatttca ccttcttctt cggagtcggt gttaatcaga
    24601 atatcagcct gcaaccagtt gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg
    24661 gtcatggtca gcagcacttc cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc
    24721 agtgcagagg ccataccaat gccgttatcc gcacccagcg tggtgccgcg cgctttaacc
    24781 cattcgccat caatataagg ctggatagga tctttcgtga agtcatgcac ggtgtcgtta
    24841 tttttctgcg gcaccatatc gaggtgggcc tgtaagacga ccggtttacg attttccata
    24901 cctgcggtag caggttgacg aatcaggata ttacctacct gatcgcgttc gacatggaaa
    24961 cctttctctt ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg ataggacggg
    25021 tgaggaatag aacagatttt ggcaaaaata tcccacagcg gctgtggaga taattgagac
    25081 agttcagaca cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg
    25141 gttagcagaa aatgttgtca acacaagaca ggcttgcgag atatgtattg agaataccac
    25201 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
    25261 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
    25321 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
    25381 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
    25441 aaggcattat tgccgtaagc cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac
    25501 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
    25561 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
    25621 tggtggatac cggtggtact gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg
    25681 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
    25741 cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
    25801 ccggtcgcta atcttttcaa cgcctggcat tgccgggcgt tgttcttttt aacttcaggc
    25861 aggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
    25921 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
    25981 tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac catccctcac
    26041 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc
    26101 ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac gtaccgacga tgatttatac
    26161 gataccgtga ttggctaccg tggtggcaac tggatttatg agtgggccac ccaggcgatg
    26221 gtgtggcaac aaaaagcctg tacggaagaa gatccgcaac tcagtggtcg acactggctg
    26281 catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga tgacctggcc
    26341 gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc
    26401 acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat
    26461 atgccgaaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct ggatgcgatg
    26521 cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg
    26581 actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca ggactccagc
    26641 ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc
    26701 gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc ataccttgaa
    26761 tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct gttgagtgat
    26821 tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag tcgtttgggg
    26881 atgcatgatg cttccgacga agcgttacgc gtggagctga atcgctattc attaaaagtg
    26941 caaggattgc tggggcgtcg ctgcccaacg ccaatgttat caggctactg gaagaacgat
    27001 ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga cggtaaatta
    27061 ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc
    27121 ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca
    27181 gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa gagcagattg
    27241 atcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga agataatcga
    27301 ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgaa
    27361 ttctggggct ggtggatgga gctggaagcg caggaatccc gttttaccta cagctaccag
    27421 tttggtctgt tcgataaagc aggcgactgg aagagtgttc cggtaaaaga tactgaagtg
    27481 gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga actgctgacg
    27541 acgctgaatc tgaagctgga accggcgaat gattttcgtg atgaaccggt gaagttaacg
    27601 gcgtgagtga aatgtgccgg atgcatcaca tccggcaata ttcattaaaa ctgatacgtc
    27661 atcccaaccg cgacagtatc atcattatta atattcagct tattatcgct atccagttgg
    27721 ttgattttat aatcaacaaa cgctgacata tttttgttga aataatacgt agcaccgacg
    27781 tcgatataat tgaccagatc ttcatcaccg atgccctcaa tatctttccc tttcgataag
    27841 acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac cgcttcaaag
    27901 ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt ttcagaatag
    27961 aaagttgcca gataaatatt attggcatcg tatttcagac ctgttgccca tgcttctgca
    28021 cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatctga gttggtatag
    28081 gccccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt gccgaagcca
    28141 tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg cagggttaag
    28201 ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag accgctggcg
    28261 cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc caccaaattc cgggaacata
    28321 tcggtccagg cttccacgtc atacaacgcg cccaggttac gaccataatc gaaagaaccc
    28381 aaatctttat atttcaaccc ggcaaaagcg agacgcgttt tttgctgtgc agtatcactc
    28441 tccgctttat ttccggcaaa ctccgcttcc caccgaccat aaccagtcag ttgatcgtta
    28501 atttgagttt cacctttaaa accaaaacgg atataactct ggtcgccatc tttactggcg
    28561 ttatcactca tataatgcat ggctttaact ttgccataga catccagttt attaccgtct
    28621 ttattatata tttctgcagc ctgtacagat gcagatgcca caatgcccat caccactaat
    28681 gccagagtgc tctttttcat tttcattcct gattttaatt aacgcgcgaa tattcagcgg
    28741 gagagccccg ttgaaaacag gaaagttttt aacctgagat tgttaaagat atattacaga
    28801 ttaatgatat t
//
LOCUS       DAJTOW010000047        28368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTOW010000047 DAJTOW010000000
VERSION     DAJTOW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.047"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            563..1333
                     /locus_tag="OO244_002148"
     CDS             563..1333
                     /locus_tag="OO244_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5663962.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="OO244_002149"
     CDS             1324..1797
                     /locus_tag="OO244_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5663963.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="OO244_002150"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="OO244_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5663964.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="OO244_002151"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="OO244_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5663965.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGKELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="OO244_002152"
     CDS             3232..3726
                     /locus_tag="OO244_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5663966.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3980..6271
                     /gene="opgB"
                     /locus_tag="OO244_002153"
     CDS             3980..6271
                     /gene="opgB"
                     /locus_tag="OO244_002153"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5663967.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6409..7431)
                     /gene="lgoD"
                     /locus_tag="OO244_002154"
                     /pseudo
     CDS             complement(6409..7431)
                     /gene="lgoD"
                     /locus_tag="OO244_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7570..8484
                     /locus_tag="OO244_002155"
     CDS             7570..8484
                     /locus_tag="OO244_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5663968.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="OO244_002156"
     CDS             8699..10060
                     /locus_tag="OO244_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5663969.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO244_002157"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO244_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5663970.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO244_002158"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO244_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5663971.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="OO244_002159"
     CDS             12610..13899
                     /locus_tag="OO244_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5663972.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="OO244_002160"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="OO244_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5663973.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="OO244_002161"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="OO244_002161"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5663974.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="OO244_002162"
     CDS             16225..16605
                     /locus_tag="OO244_002162"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5663975.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..17476
                     /gene="hpaH"
                     /locus_tag="OO244_002163"
     CDS             16673..17476
                     /gene="hpaH"
                     /locus_tag="OO244_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5663976.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKDHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17487..18290
                     /gene="hpaI"
                     /locus_tag="OO244_002164"
     CDS             17487..18290
                     /gene="hpaI"
                     /locus_tag="OO244_002164"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5663977.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18312..19667
                     /gene="hpaX"
                     /locus_tag="OO244_002165"
     CDS             18312..19667
                     /gene="hpaX"
                     /locus_tag="OO244_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5663978.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19677..20567
                     /gene="hpaA"
                     /locus_tag="OO244_002166"
     CDS             19677..20567
                     /gene="hpaA"
                     /locus_tag="OO244_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5663979.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20594..21531
                     /locus_tag="OO244_002167"
                     /pseudo
     CDS             20594..21531
                     /locus_tag="OO244_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21758..23316
                     /gene="hpaB"
                     /locus_tag="OO244_002168"
                     /pseudo
     CDS             21758..23316
                     /gene="hpaB"
                     /locus_tag="OO244_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23334..23846
                     /locus_tag="OO244_002169"
     CDS             23334..23846
                     /locus_tag="OO244_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5663980.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24239..26389
                     /gene="btsT"
                     /locus_tag="OO244_002170"
     CDS             24239..26389
                     /gene="btsT"
                     /locus_tag="OO244_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5663981.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26439..26642
                     /locus_tag="OO244_002171"
     CDS             26439..26642
                     /locus_tag="OO244_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5663982.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26653..27609
                     /gene="yjiA"
                     /locus_tag="OO244_002172"
     CDS             26653..27609
                     /gene="yjiA"
                     /locus_tag="OO244_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5663983.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27678..>28368)
                     /locus_tag="OO244_002173"
     CDS             complement(27678..>28368)
                     /locus_tag="OO244_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5663984.1"
                     /translation="NLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGW
                     LKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQV
                     NLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKI
                     GNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMRHEK
                     MKTQLKKLWLV"
BASE COUNT         6937 a         7422 c         7119 g         6890 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtaaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc
     3781 cttttccctg ctcctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa
     3841 agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca
     3901 atatggcgac gccattacat tgtgcttctc aaaaagctta aacgttggct aaacggcccg
     3961 gtttagtgag gatcgcccat tgtcagaact actctctttc gccctttttc tcgcctctgt
     4021 gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cgttaacggt
     4081 gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga
     4141 tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag
     4201 caaatacatc ctgccgggta tcggcattgt gctggggctg acggcggtgt tcggtgcgct
     4261 gggctggatc ctgcgccgtc gtcgtcatca tccgcaccat tttggttaca gcctgctggc
     4321 gctcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact
     4381 ggtgaaatcc cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc
     4441 gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg
     4501 gacctatttt gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa
     4561 tgaaggcctg gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg
     4621 catggtggct tctcagtgcg gcataccgct gttcgccccc tttgaaggca acgcctccgc
     4681 ctctgtctcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg
     4741 ttatcagaac tatttcgtgc agggcgcaaa tctgcgtttt gccggtaaag atgtgttcct
     4801 gaaatcgcac ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtagccga
     4861 cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa
     4921 aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga
     4981 tacccatcac ccggatggtt ttatctcgcg cacctgtaac cgcaaaaaat atgattttga
     5041 cggtaagccg aatcagtcat tcagcgcggt aagttgcagc caggagaata tcgcgacgtt
     5101 tatcaacaaa atcaaagcgt caccgtggtt taaagatact gtcatcgtcg tctcttctga
     5161 ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct
     5221 gttttttgtc attcgtggcg acaagccgca gcaaaagacg ctggcagtga agcgtaacac
     5281 gatggataac ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg
     5341 tcgtagcagt ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac
     5401 attggcgtgg aagccagata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt
     5461 caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct
     5521 gctgttgcgg gtttcagaca aacgcgtgga accgctgccg gaaagcgaat attcagcacc
     5581 gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgacagttg
     5641 ctacaagatg gcacaactct gggctccgga actggcactt tccaccgact ggtgtgtctc
     5701 gcaggggcag cttggcggtc agcaaattgt tcagcatgtt gacaaaacaa catggaagag
     5761 caagacggca tttaaagata cggtcatcga catggcgcgt tacaaaggca atgtcgatac
     5821 gctgaagatt gttgataacg atattcgcta caaagccgac agtttcatct ttaacgtcgc
     5881 cggtgcgcca gaagaggtga aacagtttag cgggatttcc cgtccggagt cgtggggccg
     5941 ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa
     6001 gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc
     6061 ggtacgcgta ggcaatgaag aacaaactct tgtgctgggc aatgaagtga ccaccaccac
     6121 actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt
     6181 ctcaaccaac gaagggaata tcctcggaca ctcgccgcgt aagctcggga tcggcatggt
     6241 ggaaattaaa gtggtagaac gtgaagggta attcctgact gatgtgtagg tcgcataggg
     6301 tgcgacctac acgccttctc cggcttacaa cacattatta agaaacaaaa aaaacagcgc
     6361 ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat
     6421 tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt
     6481 ggcgaacgga tagcgatggg ttaacatcat gtcggcagtg attttccctt ccgccatcag
     6541 tcgaccgact ttagcgaaat cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc
     6601 ttttttatgg aattccggat cggagaactg caactcacct ttaaacagac cgacaaatac
     6661 caccgtgccg ccgtgacgaa tcagattcac ggtattattc atcgcatgtt gattacctgt
     6721 cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa
     6781 atcttcggct gacgggtcca gcacgggcaa ctccagacgc gttgccacat gttcacggcg
     6841 cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc
     6901 gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc
     6961 gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgcggccg cctgcgggtc
     7021 aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc
     7081 atcctgatgc acgccaatga ctgaaatttt ttcacagcag ttggtacgcc cgcttttaca
     7141 cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac catttttaag
     7201 atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacccg
     7261 tggataacta aaaaatggtt gatttccacc ctaggcatga atatcggtac cgcaaatccc
     7321 gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt
     7381 ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct
     7441 atccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat
     7501 ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag
     7561 gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat
     7621 gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg
     7681 tacaacatca gccgcactac agtgcgtcat attctcagcc aattacgcga atgcggcgtc
     7741 ttaacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc
     7801 gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg
     7861 atgataaacc agcgtcagtt acgccctagg gaaacttttt ctgaactgca actggctcgg
     7921 gccgcgggtg tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat
     7981 cttattcaaa gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc
     8041 gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac
     8101 ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctagaacg tcatcggcta
     8161 ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac
     8221 tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgctgt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gaaccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt gggttgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tcttccccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggcgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccgaccg cctgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aagtcgcacg ctgaaagacc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcaccctccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
    17461 ctgccgcttt gtttaaggag agaacgatgg aaaacagttt taaagcggcg ctgaaagcag
    17521 gccgtccgca gattggatta tggctggggc tgagcagcag ctacagcgcg gagttactgg
    17581 ccggaccagg attcgactgg ttattgatcg acggtgagca cgcgccgaat aacgtgcaaa
    17641 ccgtgctcac ccaactacag gcgattgcgc cctaccccag ccagccagtg gtacgcccat
    17701 cgtggaacga tccggtgcaa atcaaacaac tgctggacgt aggcacacaa accttactgg
    17761 tgccgatggt acaaaacgcc gacgaagccc gtaaagcggt acgcgccacc cgttatcccc
    17821 ccgccggtat tcgcggtgtg ggcagtgcgc tggctcgcgc ctcgcgctgg aatcgcattc
    17881 ctgattacct gcaaaaagcc aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg
    17941 aggcaatgaa gaacttaccg cagattctgg acgtggaagg ggtcgacggc gtgtttatcg
    18001 gcccggcgga tctgagcgcc gatatgggtt atgccggtaa tccgcagcac ccggaagtac
    18061 aggccgccat tgagcaggcg atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc
    18121 tgatcgccaa tgagcaactg gcaaaacgct atctggaact gggcgcgctg tttgtcgccg
    18181 tcggcgttga caccaccctg ctcgcccgcg ccgccgaagc gctggcagca cggtttggta
    18241 atagttcttc tatttcgatg gcgaagcaaa acaacaactc tgtctactaa acgtacgatg
    18301 tggagcgcat catgacgacc tctaccctgc aatataaaga taataaaact gttgaaattg
    18361 aacatcgtgt cattaataaa ttatttcgtc gcctaatcat ttttcttttt attctgtttg
    18421 ttttttcgtt tcttgatcgc atcaatatag gatttgccgg actgacaatg ggcaaggatc
    18481 ttggcctgac gtccacaatg tttggcctcg ccgcaacact cttttatgtc acgtacgtca
    18541 tgtgcgggat ccccagtaat atgatgttag cgaaaattgg cgctcgtcga tggatcgctg
    18601 ggatcatggt ggtctgggga attgcttcca cctgcacaat gtttgcaaca agccccgaaa
    18661 cactctatct actgcgtatg ctggttggta ttgctgaggc gggtttttta cctggtattt
    18721 tggtttatct gacctggtgg ttcccggcat atcatcgggc cagagccaat gccttattta
    18781 tgatagcgat gccagtcact atgatgctgg gattgatcct ttcaggctac attctggcct
    18841 tggatggact gtggaatcta aaaggctggc aatggctctt tttgctggaa ggtctgccat
    18901 cggtcattct tggtgtcgtc acctggtttt atctcaacga cacaccagac caggcgacct
    18961 ggcttgatga aaacgaaaaa caggctttaa aaacgatgat tctccgtgaa caagagagtg
    19021 catttgccca tgcatctgtt ccacgctcaa ccttacgtga agtcctgacc ccagccatca
    19081 tgctatatac acttgcttat ttttgcctga ctaatactct gagtgcgatt aatatctgga
    19141 cgccgcaaat cctacagagt ttcaatactg gcagtagcaa catcatgatt ggtttgctgg
    19201 cggccatccc ccaattctgc acaatttttg gcatgatctg gtggagtcgt cgttccgacc
    19261 gtcttaaaga acgcaaaaag catacgattc ttccctacct gtttgccgct gccggatggt
    19321 tactcgcttc tgcgacagat cacagcctca ttcaactgag cagcatcatt atggcctcaa
    19381 tgggatcatt taccgcgatg actatctttt ggacaacacc ggatcaagtt ataagcttgc
    19441 aatcgcgtgc cgttgcgatg gctgtcatca atgctgtcgg caatgtaggt tcagcagtca
    19501 gtccattact tatcggtatt ttgaaagatg caaccggcag ttttagctca ggtttgtggt
    19561 ttgtagcagg attgctggta ctgggcgcgt tagtcatgac ccgaatccct atggggagtc
    19621 gggaacactc tctttcatct gaacttatcg caccaaaacc gcaataagga gtcatgatgt
    19681 ccagtgaagc tatctttgaa catggttatg aaaatattga tttggtccgg gaatatgatg
    19741 cccgctacgc tggtgaagat gtgcattatg aaacatttgc ccggttagcg aatttctttg
    19801 gacgcgatat gcgccctcac tggcacgata gatattttca actccattat ctggtcactg
    19861 gacgcattac tttacaactg gatgagcact tctattctgt gcgcgcaccg ctttttgtac
    19921 taacaccacc atcggtgcca catactttct ttactgatcc tgatagcgat gggcatgttc
    19981 tgaccgtacg tcaggagtta atttggcccc tggtagaaaa gctctggcct ggtagtggag
    20041 atgccgcaat gctacaaggt atctgtctgt cactggaggg aatggaacat acactggcgg
    20101 cattaaatca ttattggccg ataatatccc tggagtttca acaaaataag caaggacggg
    20161 aaattctatt gcaatcactg gcgcaaagta tttttacaat cctactgcgt gaagcaccac
    20221 caaatgatat ttcaacatgc agcgtgcgcg gcgagatgtg tttattccag aagtttaatc
    20281 ggctgattga cgaaaattat cgccaacatt taatggtccc tgaatatgca acaatgctcg
    20341 gtatgagtga atcacggctt accgagctgt gccgacgttt tgccaatcaa tcgccgaaga
    20401 gattgatttt cgaacgagtg agccgggaag ccagacggct tctgctctat agcgagcaaa
    20461 gcatcaacca gatagccctt gatcttggct acaaagatcc cgcctatttc gcccgttttt
    20521 tcaaccgaat gatgggttgc tcaccaacac aattccgtgg tcgctgacca tttatcaacc
    20581 tgagagactc gacatgaaaa tatctttacg ctcactattt gtggcgtgcc tgattgcgcc
    20641 agtggcattc acctacgcct ccgaaccgca ataaatgaaa attgtactac ttggaacgaa
    20701 aggtggccct tctttattaa aagctgaccg tttaccgatg gcaacggcac ttattattgg
    20761 caataaaacc tggttaattg atgctggtta cggggccagc ttacaactgg taaaaaataa
    20821 tattccactg cgccagattg ataatatttt actcacccat ttacacagtg atcatattct
    20881 ggactatcca tcagtcttaa tgaatgcatg ggctagcggt ctgcaggaca aaactattca
    20941 tgtattcggt ccgccaggca cccgagccat gaccgctgcc agttggcagg tcttcgatcg
    21001 ggacatcaca ctgcgtatgg aagaagaagg aaaacccgat cctcgcaaac tcgttacagc
    21061 tcaagatgtt ggtgaaggcg tagtttataa agatgatacc gtgaccgtga ctgcattaaa
    21121 ggtccctcat tctcctttcc ctgacggtga ggcttttgct tatcgcttcg acacacaagg
    21181 caagcgaatt gtcttctcag gagatacctc gtactttccc ccactggcaa ctttctcaga
    21241 aggcgctgat atacttgtgc atgaggccgt acatgagccc tccgtcgcta agctggcaga
    21301 cagtattggc aatggtaaaa cactggcaaa agccattgct tctcaccata cgactattga
    21361 ggatgtaggc aaaattgcca atgaagcaaa agtgaaaaaa ctcatcctta gccacctggt
    21421 accaatcacc gttccggatg aaacatggaa gcaagaagca cagaaaacta ttcaggccct
    21481 atcgtcgttg gtcatgaaaa tatgacgatt accattccat tcgccccata aaaaattccg
    21541 tccccgttag cccttgttag cggggaatat tatgatttgc cttactgtaa gttgatcacg
    21601 atcgcagttt actatttaac tccaccttgc tgtcgaaaag taccaacgtt cgcctgaaac
    21661 gtctcttccc gacaaccgcg ttcaacgcct caattaaata acaaaaacaa cacacaaaat
    21721 taacaatcgt attccgatta atactgtaga ggtcgacatg aaaccagaag atttccgcgc
    21781 cagtacccaa cgtcctttca ccggggaaga gtatctgaaa agcctgcagg atggtcgcga
    21841 gatctatatc tatggcgagc gagtgaaaga cgtcaccact catccggcat ttcgtaatgc
    21901 ggtagcgtct gttgcccaac tgtacgacgc gctgcacaaa ccggagatgc aggactctct
    21961 gtgttggaac accgacaccg gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa
    22021 aagtgccgac gacctgcgcc agcaacgcga cgccatcgct gaatggtcac gcctgagcta
    22081 tggctggatg ggccgtaccc cagactacaa agccgctttc ggttgcgcac tgggcgcgaa
    22141 tccgggcttt tacggtcagt tcgagcagaa cgcccgtaac tggtataccc gtattcagga
    22201 aactggcctc tactttaacc acgcgattgt taacccaccg atcgtcattt gccgaccgat
    22261 aaagtgaaag acgtttacat caagctggaa aaagagactg acgccgggat tatcgtcagc
    22321 ggtgcgaaag tggttgccac caactcggcg ctgactcact acaacatgat tggcttcggc
    22381 tcggcacaag tgatgggcga aaacccggac ttcgcactga tgttcgttgc gccaatggat
    22441 gccgatggcg tgaaattaat ctcccgcgcc tcttatgaga tggtcgcggg tgctaccggc
    22501 tcgccatacg actacccgct ctccagccgc ttcgatgaga acgatgcgat tctggtgatg
    22561 gataacgtac tgatcccatg ggaaaacgtg ctgatctacc gcgattttga tcgctgccgt
    22621 cgctggacga tggaaggcgg tttcgcccgt atgtatccgc tgcaagcctg tgtgcgcctg
    22681 gcagtgaaac tcgacttcat tacggcactg ctgaaaaaat cactcgaatg taccggcacc
    22741 ctggagttcc gtggtgtgca ggccgatctc ggtgaagtgg tggcgtggcg caacaccttc
    22801 tgggcattga gtgactcgat gtgttcagaa gcgacgccgt gggtcaacgg ggcttattta
    22861 ccggatcatg ccgcactgca aacctatcgc gtactggcac caatggccta cgcgaagatc
    22921 aaaaacatta tagaacgcaa cgttaccagt ggcctgatct acctcccttc cagtgcccgt
    22981 gacctgaaca atccgcagat cgaccagtat ctggcgaagt atgtgcgcgg ttcgaacggt
    23041 atggatcacg tccagcgcat caagatcctc aaactgatgt gggacgccat tggcagcgaa
    23101 tttggtggtc gtcacgagct gtatgaaatc aactactccg gtagccagga tgagattcgc
    23161 ctgcaatgtc tgcgccaggc acaaagctcc ggcaatatgg acaagatgat ggcgatggtt
    23221 gatcgctgcc tgtcggaata cgaccagaac ggctggactg tgccgcacct gcacaacaac
    23281 gacgatatca acatgctgga taagctgctg aaataacgca gcaggaggtt aagatgcaat
    23341 tagatgaaca acgcctgcgc tttcatgacg cgatggccag cctgtcggca gcggtaaata
    23401 ttatcaccac cgagggcgac gccggacaat gcgggattac ggcaacggct gtctgctcgg
    23461 tcacggatac accaccgtcg ctgatggtgt gcattaacgc caacagtgcg atgaacccgg
    23521 tttttcaggg caacggcaag ttgtgcgtca acgtcctcaa ccatgagcag gaactgatgg
    23581 cgcgccactt cgcgggcatg acaggcatgg cgatggaaga gcgttttagc ctctcatgct
    23641 ggcaaaaagg tccgctggcg cagccggtgc taaaaggttc gctggccagt cttgaaggag
    23701 agatccgcga tgtgcaggcg attggcacac atctggtgta tctggtggag attaaaaaca
    23761 tcatcctcag tgcagaaggt cacggactta tttactttaa acgccgtttc catccggtga
    23821 tgctggaaat ggaagctgcg atttaagctc cagccccttc acgcattcgt cgaaggggca
    23881 tttttattgt gatctcaatc acatatctac ccgatgaata attcttgccc aaccgctcac
    23941 cgccattcct gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg
    24001 cgcacgtatc tctggcaaca tcactgcagg atagcggtca atttacctcc tcaaacgcaa
    24061 cgcaaaccta gaacggcttc ggcctatgat taacaaatta ctctatggat tttggtttgc
    24121 aggaaggcag caagtgagtg aatccccggg agcttacaaa agtaagtgtc agaggtgaac
    24181 gaacgcagct gccgcacctg taagccaaaa gacgacgagt aaaatgccag gttttactat
    24241 ggatacgaaa aaactattca agcacatacc ctgggtgatt ctcggaatca tcggtgcatt
    24301 ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt
    24361 cgcctctgtg tcggtgtatc tggtggcgta tcgctactac agtctgtaca tcgcccagaa
    24421 ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa
    24481 ctacgttccg accaaccgtt acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc
    24541 tggtccgctg gtgggaccgg ttctcgccgc gcagatgggc tacctgcctg gcacgctgtg
    24601 gctgctggcg ggggtagtgc tggccggtgc ggtacaggac tttatggtgc tgtttatctc
    24661 ctctcgccgt aacggcgcat ctctcggtga gatgatcaaa gaagagatgg ggccagtacc
    24721 ggggactatc gcgctgtttg gctgtttctt aatcatgatc atcatcctcg ccgttctggc
    24781 gctgattgtg gttaaagccc tggccgaaag tccgtggggt gtcttcaccg tttgctcaac
    24841 cgtaccgatt gcgctgttta tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg
    24901 tgaagtctct gttattggta tcgtgctgct ggttgcctct atctacttcg gtggcgtgat
    24961 tgctcacgat ccgtactggg gtccggcact gacctttaaa gacaccacca ttaccttcgc
    25021 gctgattggc tatgcgtttg tttccgcact gctgccggtg tggctgatcc tcgcaccgcg
    25081 cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt
    25141 ggtgctgaac ccggaactga aaatgcctgc gatgacccag tacattgacg gtactggccc
    25201 gctgtggaaa ggcgctctgt tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc
    25261 tggcttccac gcgctgatct cttccggtac gacgccgaaa ctgctggcta acgaaaccga
    25321 cgcgcgtttc attggctacg gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct
    25381 ggttgcagcg tccatcatcg aaccgggtct ttacttcgcg atgaacaccc cgcctgctgg
    25441 ccttggcatc accatgccta acctgcatga aatgggtggc gagaacgcgc cgatcatcat
    25501 ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt
    25561 gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa
    25621 ccgtgcaggt ggtgcgccga cgctggcggt aggtatcgct cacgtgttcc acaaagtgct
    25681 gccgatggct gacatgggct tctggtatca cttcggtatt ctgttcgaag ccctgttcat
    25741 cctgaccgcg ctggatgcgg gtacccgttc tggccgcttt atgctgcaag acctgctggg
    25801 taacttcatc ccgttcctga aaaaaaccga ctctctggtt gccggtatca tcggtactgc
    25861 gggctgtgtg ggtctgtggg gctacctgct gtatcagggc gtggtcgatc cgctgggcgg
    25921 cgttaagagc ctgtggccgc tgttcggtat ctccaaccag atgctggcag ccgtagcgct
    25981 ggtactgggc accgttgtgc tgattaagat gaagcgcacc caatacatct gggtaactgt
    26041 tgttccggct gtatggctgc ttatctgcac cacctgggcg ctgggtctga aactgttcag
    26101 caccaacccg cagatggaag gcttcttcta catggcaagc cagtacaaag agaagattgc
    26161 taacggtact gacctgacgg cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa
    26221 ctacaccaac gcaggcctga gtattctgtt cctgattgtg gtgtacagca tcatcttcta
    26281 cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc
    26341 gtacgttcca atcccggaag gcggcgtgaa gatctcttcg caccactaac cgtgtttagc
    26401 cccgcttcgg cggggctttg ttcttatcag agtgaactat gtttggtaac ttaggacagg
    26461 caaaaaaata tctcggccag gcggcgaaga tgttgattgg tattccagac tacgacaact
    26521 acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct
    26581 tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt
    26641 aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc
    26701 aaaactaccc tgctgcgcca tattcttaac gaacaacacg gctacaagat tgccgtgatt
    26761 gaaaacgaat tcggcgaagt ctccgttgat gaccaactga ttggcgaccg cgccacgcag
    26821 atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaggacgca
    26881 ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgatcg tctggtgatt
    26941 gaatgcaccg gcatggccga tcccggcccg attattcaga cctttttctc ccatgagatt
    27001 ttatgccagc tctacctgct ggacggcgtg attgcgctgg tggatgcggt acatgccgat
    27061 gagcagatga accagttcac catcgcccag tcccaggtgg gctacgccga ccgtattctg
    27121 ctgactaaaa ccgacgtcgc aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc
    27181 aacgcccgcg caccggtcta caccgtcacc cacggcgaca tcgacctggg cctgctgttc
    27241 aacaccaacg gttttatgct ggaagagaac gtcgtcagca ccaaaccgcg tttccacttt
    27301 atcgcggata aacaaaacga tatttcgtca attgtggtgg aactggatta cccggtagat
    27361 atcagcgaag tttcccgcgt gatggaaaac ctgctgctgg aatcggcgga taaactgctg
    27421 cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctgtt ccagggcgtc
    27481 cagcgcctct acagcgccga ctgggacagg ccgtggggcg atgaaaaacc gcatagcaca
    27541 atggtgttta tcgggattca gttgccggag gaagagattc gggcggcgtt tgcgggattg
    27601 aggaagtaag ttcgggaaag atcaactttt tgagagttcc accgcaacat tattacgagt
    27661 ggaactcttt agatatgtca gaccaaccaa agcttcttca attgcgtttt cattttttca
    27721 tgtctcattt tcagttcagc caatccccat tctgaagaat tacaaacatc ttttgttaaa
    27781 taaaaagagc ttttactatt aagtttctca taaatagatt tctttttgat aaaatcatag
    27841 tgttgtgttt cagaattttt ggaaccactt atgagtgtta aattacctat tttatgcagc
    27901 cagtagacat gctcttgagg ttggaatcta ttaatccagt attcattaac taatgcttga
    27961 ggaagaatat gctcaattgt gattctcccc gtataggttt taatcacgct ctcatcttgt
    28021 tgctccatat ctaagcgaag caaaattgct tttattaagt tcacttgatt cggacgaggc
    28081 tcatataaat cttcatccag agcagctata aaacctgaat tatctgcatg ttggtttatc
    28141 tgatttatca cagaatcaaa aggcatatca ttatttattg ctaccagagc agaataacaa
    28201 accatttctc tttggctttt aatttgtttt tttaaccacc catgcatata taccttctca
    28261 aaggcggtaa taaattgaga aaaatcatct aaattaaaat cttctgtacg agccattcga
    28321 ttcatgaatg ccataaccgg aggaatccac tcatctaccc ctaaatta
//
LOCUS       DAJTOW010000048        28090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTOW010000048 DAJTOW010000000
VERSION     DAJTOW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.048"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO244_002174"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO244_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5663985.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="OO244_002175"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="OO244_002175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5663986.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="OO244_002176"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="OO244_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5663987.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="OO244_002177"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="OO244_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5663988.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="OO244_002178"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="OO244_002178"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5663989.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="OO244_002179"
     CDS             6428..7057
                     /locus_tag="OO244_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5663990.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO244_002180"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO244_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5663991.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLYPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="OO244_002181"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="OO244_002181"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5663992.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMGSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="OO244_002182"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="OO244_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5663993.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="OO244_002183"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="OO244_002183"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5663994.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="OO244_002184"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="OO244_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5663995.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="OO244_002185"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="OO244_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5663996.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="OO244_002186"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="OO244_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5663997.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="OO244_002187"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="OO244_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5663998.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="OO244_002188"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="OO244_002188"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5663999.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO244_002189"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO244_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5664000.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO244_002190"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO244_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5664001.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="OO244_002191"
     CDS             complement(21748..22626)
                     /locus_tag="OO244_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5664002.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     ARSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO244_002192"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO244_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5664003.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO244_002193"
     CDS             25252..25587
                     /locus_tag="OO244_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5664004.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO244_002194"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO244_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5664005.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO244_002195"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO244_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5664006.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..>28090
                     /gene="phnD"
                     /locus_tag="OO244_002196"
     CDS             27134..>28090
                     /gene="phnD"
                     /locus_tag="OO244_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5664007.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDD"
BASE COUNT         7225 a         7333 c         6841 g         6691 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcactc tcaagccgtt aattcttaac accggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggtacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aacccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccacgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gttgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gcgctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 cctggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgcctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac
//
LOCUS       DAJTOW010000049        27556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTOW010000049 DAJTOW010000000
VERSION     DAJTOW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.049"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO244_002197"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO244_002197"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5664008.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO244_002198"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO244_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5664009.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO244_002199"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO244_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5664010.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO244_002200"
     CDS             3807..3995
                     /locus_tag="OO244_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5664011.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO244_002201"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO244_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5664012.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO244_002202"
     CDS             4756..5115
                     /locus_tag="OO244_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5664013.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO244_002203"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO244_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO244_002204"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO244_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5664014.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO244_002205"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO244_002205"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5664015.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10895..11071
                     /locus_tag="OO244_002206"
                     /pseudo
     CDS             10895..11071
                     /locus_tag="OO244_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11399..13147
                     /gene="bcsC"
                     /locus_tag="OO244_002207"
                     /pseudo
     CDS             11399..13147
                     /gene="bcsC"
                     /locus_tag="OO244_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13229..15217
                     /gene="hmsP"
                     /locus_tag="OO244_002208"
     CDS             13229..15217
                     /gene="hmsP"
                     /locus_tag="OO244_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5664016.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPYAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15402..16688
                     /gene="dctA"
                     /locus_tag="OO244_002209"
     CDS             15402..16688
                     /gene="dctA"
                     /locus_tag="OO244_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5664017.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            16909..18405
                     /locus_tag="OO244_002210"
     CDS             16909..18405
                     /locus_tag="OO244_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5664018.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OO244_002211"
     CDS             complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OO244_002211"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5664019.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19662..20429
                     /gene="pdeH"
                     /locus_tag="OO244_002212"
     CDS             19662..20429
                     /gene="pdeH"
                     /locus_tag="OO244_002212"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5664020.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20499..22559
                     /locus_tag="OO244_002213"
     CDS             20499..22559
                     /locus_tag="OO244_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5664021.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22741..24063)
                     /locus_tag="OO244_002214"
     CDS             complement(22741..24063)
                     /locus_tag="OO244_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5664022.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OO244_002215"
     CDS             complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OO244_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5664023.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25536..26435)
                     /locus_tag="OO244_002216"
     CDS             complement(25536..26435)
                     /locus_tag="OO244_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5664024.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26955..>27556
                     /locus_tag="OO244_002217"
     CDS             26955..>27556
                     /locus_tag="OO244_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5664025.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6563 a         6954 c         7555 g         6484 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac
    11221 gttaatgcgc aatctggcgc agcagaagcc gaacaacccg gagcaggttt acgcttacgg
    11281 gctgtacctc tcaggtcaca accaggacag agcggcgctg gcgcatatca acagcctgcc
    11341 gcgcgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt
    11401 gctggaaacc gctaatcgcc tgcgagaaag cggcaaagag gctgaagcgg aagcgatgct
    11461 gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg cgtaacaacg
    11521 acgtgattac accgccgccc gcactgcata tcagaatgtc ctgacgcggg agccaactaa
    11581 cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc
    11641 acgtagccag ctggcgaaac tgcctgccac cgataacacc tcgctgaaca ctcagcggcg
    11701 cgtggcgctg gcacaggcgc agcttggaga taccgcagcg gcgcagcaga cgtttaataa
    11761 gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tactgcgcga
    11821 cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa cctacaaaga
    11881 cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca cctttacccg
    11941 actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca gcgatgcggc
    12001 ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact ggggttcgag
    12061 cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc
    12121 gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt
    12181 ctccactaat gccgatggca aatgggatga caactggggc acctgtacat tacaggactg
    12241 tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga
    12301 cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg
    12361 cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg
    12421 gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg
    12481 gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg
    12541 tgaagtaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga
    12601 agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa
    12661 tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg
    12721 ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc
    12781 ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt ctggctcgtg
    12841 gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga
    12901 ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg gcttcggcta
    12961 cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg gcacggcaat
    13021 tgatatccag caggtgaaag attacgcacc cagccatttc ctgctctacg tacgttattc
    13081 cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac cttacgccga
    13141 ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat actcgggcgg
    13201 caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa caatcaagca
    13261 gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca ccgttttgct
    13321 gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa gcattgctcg
    13381 ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg aagcggaagc
    13441 tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa
    13501 ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg taatggttac
    13561 tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc
    13621 aaatccgcag ccaatagcct atctggtgct acaggcggat tccttccgta tgtataagtt
    13681 cgtgatgagc actctctcaa cgttagtgac catttactta cttttgtcgc ttatcctgac
    13741 cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca atattgctcg
    13801 cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat taccgcgtct
    13861 gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc agcaattgct
    13921 gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc
    13981 gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc
    14041 gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca
    14101 acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt cgccacgtat
    14161 gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg tacaggaacc
    14221 gtggcacgca atcactttgg gtcagcaagt actcactatc atgagcgagc gcctgccgat
    14281 tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct acggcgatct
    14341 caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc gccataaagg
    14401 caagaatcag attcagttct ttgatccgca gcagatggaa gcagcccaga agcggttgac
    14461 ggaagagagc gatatcctta atgcactgga aaatcatcag tttgcaattt ggttacagcc
    14521 acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc gtatccagca
    14581 accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt gctgtgggct
    14641 gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg cagcctggca
    14701 agagcgcggc attatgctgc ccttgtcggt aaacctctct gcgctgcaac tgatgcaccc
    14761 gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc cgggaacact
    14821 gattctggaa gtgacagaaa gccgacgtat tgacgaccct tatgctgcgg tggcaatcct
    14881 ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca tgggctacgc
    14941 agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga aaatcgacaa
    15001 aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta tcatgctggc
    15061 gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac aacgagactg
    15121 gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgcc cactccctat
    15181 tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg attacaaaac
    15241 tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt gtttcaatta
    15301 tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc aggtaccccc
    15361 cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc tctctgttta
    15421 aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct
    15481 atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga
    15541 tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca
    15601 tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca
    15661 tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg
    15721 tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc
    15781 agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca
    15841 gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc
    15901 tgggcagcaa aggccaactg atttttaacg tgatcgaaag tttctcgcag gtcatcttcg
    15961 gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gctcggggca atggcgttta
    16021 ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct
    16081 acattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt
    16141 tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta ctggggactt
    16201 catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta
    16261 aatcggtggt ggggctggtc atcccgacag gctactcgtt taaccttgat ggcacatcga
    16321 tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg
    16381 tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa
    16441 cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag
    16501 cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctaacta
    16561 acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc
    16621 acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat
    16681 cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata
    16741 attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg
    16801 tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc
    16861 gacaacgggc agttttttta ttaccaggat agttgatcag gggttcacat gcagggcata
    16921 aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca
    16981 gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg
    17041 caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat
    17101 accggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt
    17161 gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg gcagcagggg
    17221 atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat
    17281 ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta tctggcaaat
    17341 gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca aagtcaggac
    17401 atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg tctgaaaggg
    17461 tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaaa
    17521 attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact ggtggtggga
    17581 aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc
    17641 aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt
    17701 attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag
    17761 ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgcgaggcg
    17821 ctgttctggc atgttcagca agcgttaagc gccagtgaca gcaaagacat cggtcttgga
    17881 tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac
    17941 gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa
    18001 ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa
    18061 ctgtttgccg cctatgcacg ggctgatacc gatattctga tgggccagcg gatgcgttcg
    18121 ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg gcaggatttc
    18181 cttaacagcc tgactgtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat
    18241 atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag
    18301 gcggcctggg atcaaatcat ggccccatct accgccgctg cgaccacctc tgtcgccacg
    18361 gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg
    18421 agttagcaca accatcaggt atttatggat gagctggata gaggggtaac actttatcca
    18481 gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat cgcgccgcga
    18541 tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg
    18601 cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg
    18661 atcacttttt ctttcggcag tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg
    18721 gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg
    18781 gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc
    18841 gtcaggaagg cgatatccgt gcattccagc atttgctggt acacctgctg tgtctcttct
    18901 ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg
    18961 cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg
    19021 ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac
    19081 tgctcgctcg ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt
    19141 tcgccggtgc tgtcggtttc aatgtagtaa aggcccggaa gacggttttc catccgttgg
    19201 gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg
    19261 tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg
    19321 atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc
    19381 tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat agtcgtgaac
    19441 ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta
    19501 aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag
    19561 tgaggaaatt taccccggcc aggcggtaaa gttctgccct tacgcgccga taatctttgt
    19621 cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc
    19681 agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc
    19741 agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg
    19801 tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc
    19861 gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag cagattgatt
    19921 tgctggcgca aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg
    19981 atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc
    20041 ttccctggct gcgtttcgaa ctggtggagc atatccgtct gccgaaagat tcaacctttg
    20101 cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt
    20161 tctctgcact aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga
    20221 tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat ctaatgaatc
    20281 gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc
    20341 agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatggaaa
    20401 cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgccggg ctggactatc
    20461 tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa
    20521 ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa
    20581 cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc
    20641 gtccgttcgc tatccgtggc aatctgggcg tggtgtggga gcggcaaaaa caagaaactg
    20701 gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt cttggtaatc
    20761 caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca acgctggcac
    20821 cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa aagcccgacg
    20881 cgcgcctgat tcgcctctct gaaaagaaca ataactggac gtttaatctt gccaacgatg
    20941 acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat aatattcttt
    21001 tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg
    21061 ttgatccgtt aggcaagccg ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg
    21121 ataaagaaaa ggtgggcgat tacatttttg gcctgaaggc gcagggacgt tataacggtg
    21181 aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc gaagggacgc
    21241 cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt gatggcgtcg
    21301 tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct ggcgattcac
    21361 tgggtgatct ctatgaattg acgggcgttc tgctgcccga taccccgccg tttgaaacgg
    21421 atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt
    21481 ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc accggaaagc
    21541 cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac
    21601 cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga
    21661 agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg
    21721 ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca
    21781 gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc
    21841 tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt catctggaag
    21901 gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag
    21961 aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg
    22021 aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga
    22081 aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg
    22141 tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg
    22201 cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg
    22261 agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcggaacag ctggatttga
    22321 ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg ctgtatgtgc
    22381 gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt gcccgtggtg
    22441 ctgttgctgc ggcactggca acgctggtaa caccggcggc ggcgttactg gcactgatct
    22501 caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat
    22561 gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt ggcaaagtgt
    22621 gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt
    22681 caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt ttcacactta
    22741 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    22801 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    22861 gatatacggc gcaacggaag ccccgagaat cgacgatacg ttgtaagaga acgatgctcc
    22921 ggtgtaacgc acttctgtcg gaaacagctc tggtaacagc gcacccatcg gcccgaaggt
    22981 cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    23041 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac
    23101 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    23161 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    23221 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    23281 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    23341 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    23401 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    23461 tggcacacgc cagccccagc tcataaactg ctcatcggtc agtagccagg aaagcagcaa
    23521 gaaagtgccg ttggcaaaga agaagccaat cggtgcgcct agctgtggga aggagccata
    23581 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc cgccccattc
    23641 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat
    23701 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    23761 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    23821 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    23881 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    23941 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    24001 attacgcgag ttgatcggcg tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg
    24061 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    24121 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    24181 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    24241 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggag
    24301 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    24361 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    24421 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    24481 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    24541 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    24601 aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat cacgatttta
    24661 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    24721 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    24781 atcgccaggc caatcaatcg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    24841 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc
    24901 agcgcaatca acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    24961 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    25021 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    25081 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga
    25141 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    25201 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    25261 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgcg
    25321 cgaataaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    25381 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt aatcggctcg
    25441 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    25501 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    25561 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    25621 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    25681 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    25741 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    25801 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    25861 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    25921 agatagtgcg gactggcaca gttgaccatg gttagtttgc cgaggggacg ggcgataacg
    25981 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    26041 accggacggt tatggctact cagttccagc tcaattcccg gatgctgata gagaaattcc
    26101 gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat gcgtaattta
    26161 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    26221 acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta
    26281 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    26341 cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    26401 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    26461 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    26521 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    26581 atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct ttaaagtgaa
    26641 tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt tattttggct
    26701 tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    26761 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    26821 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaacgcc aaatggcgaa
    26881 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    26941 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    27001 taaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggtc agtcaggcag
    27061 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    27121 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta
    27181 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt atccatttta
    27241 atgtgctggc cattgtgcct cgcgattcaa ccgtcgagac atttgcgttg gcggtaaata
    27301 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    27361 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    27421 gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    27481 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    27541 taaatatctc ctcctg
//
LOCUS       DAJTOW010000050        27535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTOW010000050 DAJTOW010000000
VERSION     DAJTOW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.050"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO244_002218"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO244_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5664026.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO244_002219"
     CDS             complement(1614..1937)
                     /locus_tag="OO244_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5664027.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO244_002220"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO244_002220"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5664028.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO244_002221"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO244_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5664029.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYLYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO244_002222"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO244_002222"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5664030.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO244_002223"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO244_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5664031.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO244_002224"
     CDS             7537..8574
                     /locus_tag="OO244_002224"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5664032.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGVPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO244_002225"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO244_002225"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5664033.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO244_002226"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO244_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5664034.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO244_002227"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO244_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5664035.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO244_002228"
     CDS             10745..11626
                     /locus_tag="OO244_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5664036.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO244_002229"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO244_002229"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5664037.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO244_002230"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO244_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5664038.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO244_002231"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO244_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO244_002232"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO244_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5664039.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO244_002233"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO244_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5664040.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO244_002234"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO244_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5664041.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO244_002235"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO244_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5664042.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO244_002236"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO244_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5664043.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO244_002237"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO244_002237"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5664044.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO244_002238"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO244_002238"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5664045.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO244_002239"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO244_002239"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5664046.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO244_002240"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO244_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5664047.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO244_002241"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO244_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5664048.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO244_002242"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO244_002242"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5664049.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO244_002243"
     CDS             24893..25273
                     /locus_tag="OO244_002243"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5664050.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO244_002244"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO244_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5664051.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO244_002245"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO244_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5664052.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27535
                     /locus_tag="OO244_002246"
                     /pseudo
     CDS             26442..27535
                     /locus_tag="OO244_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6793 a         6851 c         7222 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagctg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gaggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag gggtaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg aacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgta
//
LOCUS       DAJTOW010000051        26563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTOW010000051 DAJTOW010000000
VERSION     DAJTOW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.051"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            347..640
                     /locus_tag="OO244_002247"
     CDS             347..640
                     /locus_tag="OO244_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5664053.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="OO244_002248"
     CDS             712..1371
                     /locus_tag="OO244_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5664054.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1463..1909
                     /locus_tag="OO244_002249"
     CDS             1463..1909
                     /locus_tag="OO244_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5664055.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="OO244_002250"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="OO244_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /locus_tag="OO244_002251"
     CDS             3389..3808
                     /locus_tag="OO244_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5664056.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /gene="umuC"
                     /locus_tag="OO244_002252"
     CDS             3808..5076
                     /gene="umuC"
                     /locus_tag="OO244_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5664057.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQCTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="OO244_002253"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="OO244_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5664058.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="OO244_002254"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="OO244_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5664059.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="OO244_002255"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="OO244_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5664060.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="OO244_002256"
     CDS             complement(8331..9863)
                     /locus_tag="OO244_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5664061.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /gene="dadA"
                     /locus_tag="OO244_002257"
     CDS             10193..11491
                     /gene="dadA"
                     /locus_tag="OO244_002257"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5664062.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="OO244_002258"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="OO244_002258"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5664063.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14153)
                     /locus_tag="OO244_002259"
                     /pseudo
     CDS             complement(12739..14153)
                     /locus_tag="OO244_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14764..16500)
                     /locus_tag="OO244_002260"
     CDS             complement(14764..16500)
                     /locus_tag="OO244_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5664064.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16595..17509)
                     /gene="ldcA"
                     /locus_tag="OO244_002261"
     CDS             complement(16595..17509)
                     /gene="ldcA"
                     /locus_tag="OO244_002261"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5664065.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17609..18220
                     /gene="emtA"
                     /locus_tag="OO244_002262"
     CDS             17609..18220
                     /gene="emtA"
                     /locus_tag="OO244_002262"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5664066.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18222..18956)
                     /locus_tag="OO244_002263"
     CDS             complement(18222..18956)
                     /locus_tag="OO244_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5664067.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19157..19411
                     /locus_tag="OO244_002264"
     CDS             19157..19411
                     /locus_tag="OO244_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5664068.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19461..20921)
                     /locus_tag="OO244_002265"
     CDS             complement(19461..20921)
                     /locus_tag="OO244_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5664069.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20858..21415)
                     /locus_tag="OO244_002266"
     CDS             complement(20858..21415)
                     /locus_tag="OO244_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5664070.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21441..22295)
                     /locus_tag="OO244_002267"
     CDS             complement(21441..22295)
                     /locus_tag="OO244_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5664071.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22295..23104)
                     /locus_tag="OO244_002268"
     CDS             complement(22295..23104)
                     /locus_tag="OO244_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5664072.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23114..23872)
                     /locus_tag="OO244_002269"
     CDS             complement(23114..23872)
                     /locus_tag="OO244_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5664073.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23869..24849)
                     /locus_tag="OO244_002270"
     CDS             complement(23869..24849)
                     /locus_tag="OO244_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5664074.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24849..25871)
                     /locus_tag="OO244_002271"
     CDS             complement(24849..25871)
                     /locus_tag="OO244_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5664075.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26221..>26563
                     /locus_tag="OO244_002272"
     CDS             26221..>26563
                     /locus_tag="OO244_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664076.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6676 a         6468 c         6854 g         6565 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcag cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcttgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct gcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagacc actgtatata
     3361 aaaacagtat aacttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagtgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaatgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg cagctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca acaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa ccccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agcggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgtccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttcatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg agtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agtccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggtg ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcact
    12541 ggcgttacgc gtcccggttg tgacagtgta acttgttgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attgttgact cggagtcgac
    12781 ttcagataaa tcacgcttca cccttgatgc agattcgggt tcatgttcag attgttgatt
    12841 aacaggctgc gagtcctgtt gtctttccag actcaacagg cccaactgtt gatccaggag
    12901 ctccaggtcc tcctgtacct tcacgcgctc caggagttcc gggtcatcca ggaactccag
    12961 atccataagg tcctccagct cctcgagtcc ttccaggtca acctagcctt ccttgtgcaa
    13021 aaagtccaaa aggtccttca ggccctgctg ttttttcagg ccctcgacgg ctttgagcag
    13081 cgtcggtaaa ttgtctttgt cccacgggtt accattacag tcaaacacct tcaggctctg
    13141 gggctgcggc acctgtaccg ttttaaactt gttttctcca cagtagtaaa ttattagttt
    13201 gggcggcagc cgtggtgaag agatcaaatc gttactagaa cagtcaaata ctttcaggcg
    13261 agtcaagttc tccagcatct catcaggtat tgagttaata ttgttgccgg cacaggatag
    13321 tttatacagg tactggggca tcgacggcgg taaaaattgc aagaagtttt cagaacagtt
    13381 aatcactctc aggttagtcg acagctcagg cagccagata atactgtttt catagcactt
    13441 cagtaccatc agttttgagg gctgcacctt cggtaacaag atcaagacgt tatctgtaca
    13501 gtcaagcacc agcagccact ttggcatcac cttcggtaac gcggtcaaca cgtttttact
    13561 gcagtaaaat tcaatcagat gtggaggcaa attctcaggg agtgaagtca atcccaggtt
    13621 gctaagattg agcatactcc gtcttgtaat gagacattcc ttcattctgc gcaatgcttc
    13681 tgcccggttt tcaatccggc ttgctggcag gttttcttcc aggatatttt ccttgatttc
    13741 ttccagactt tctttcaggt cttcttgggg aatttctgac tgtttgtttt gctggttttc
    13801 ttccaggatt tcgtacaggt ttttttgcag gttttcttgc aaaccatcct gctgtatagc
    13861 ccattcgcac catttggcga ccatcatgaa ctcgttatgt tgctgattca gacgagtccc
    13921 caacgtgatg atggtattgt tgtccacctg tatgttcact ttgcccgcga tactgaggtc
    13981 aatgatctgc cagggggcat caccccagct gaatttttgc gggctgagat acttcatgtt
    14041 ctgcaaaatt tgattcttga gaagagccgc cctggcgggc gtcgtacgcc tgggttaacg
    14101 gaagatgggg gaatggcggg ggcgaatggg cagatgtctt taaagctagc cataatgcta
    14161 cgtttccttg aaataaatat cctctggcag cgccggagac gtgaatgtgg tcaccgcaaa
    14221 ggagccacag gacgtcattt gcgctgatat cactactcat cgttgacttt tttcctggtc
    14281 agaaatgccg tgtcgggtct ggtacatgct gtattacggc ctaggtagct ctgacgtatt
    14341 aaagaaaata cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag gaataactat
    14401 aagaaatcgc tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt gtgggctatg
    14461 tcagtgcggc aggttgcgtg ggggaatatg ttcaggcgat gtcaggcagg ggcccctgat
    14521 ggttgaccgg taatgcgggg ctggttttta ttacctgtta cagtcggaaa aagcccttac
    14581 gctgactttc ttgagccggt gttttgagtt cattagccag agcctgcaat ggtttgtcat
    14641 ccataattta accttcttct gatgttggga tgagcatatc aaaatcagga aatgacaagt
    14701 ttattacagg ctcaggctgc gttcagtaaa gcatcgcatc cggcgataga tgctgcatta
    14761 aatttaagat tcagcttcct cttcagccac ccgaacgcca atcttcaaca cttcattatc
    14821 ttctttctcg gccaccgtcc agatcatccc ggcaaactct acctggtcac cgacaaccgg
    14881 tgccgcgcct agcaactgct ggacaatttc acccagcgtt tgctgtttat cacgatattc
    14941 tcgcccgtct tccaggccat atatcagcgc aacatcagca tatttggcgc tggcttcgag
    15001 aatgaagtca ccaaagaagc gttgatccag cgcgaccggt ggcgactggc tgaacagttt
    15061 accgagcgcc gggagatcgc gttcccgacc aattacacac aatacatcgc cttcgcgcag
    15121 tcgggtgctg ccggtaggat gaagcaactg gttatcacga aacagggctg caatacgcgt
    15181 ctcttttggc atatgcaaat cgcgcagtgc cgcacctaca caccatttat cagcactcaa
    15241 ttgataaaca aactgctccc acggattttc cggatgaata tccaggccaa cgcgtgacac
    15301 cggacgtccg acgggcggaa ccaccacttt ggcttttttc gccgcccacg agagtgatgt
    15361 tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg acattaaaga acagtcgcgc
    15421 attctccagc cccgccatca tcgggaacac ggctaggatg attggcaccg cgccgcgtaa
    15481 tcccacccag ctgataaaca cgcgctcacg cagattgaaa ccgcggaaag ggagcaatcc
    15541 agcaaatacc gaaaggggac gggcgaagaa tatcatccat gccgacaaaa tgagcgccgg
    15601 aatggcaata ggcagcagat cgcttgggtt aaccaatagc cccagcacca ggaacatggc
    15661 gatttgcgcc agccaggcga ggccgtcgaa attttgcagg atgccgtagc ggttgcgaat
    15721 cggccgatta cccagcagaa aaccgcacag ataaacagcc agaataccgc tgccttccag
    15781 cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat gccagcaatg gatataatcc
    15841 ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa taaccaccgc caagaccgat
    15901 gacaatcccg aggccaaatt gctgcagaat atcgaccacg aacatccaac tgacactgct
    15961 ttcatgctgc tggatcattg caatcagggt aatcgtcaga aagaccgcca ttggatcatt
    16021 actgccggat tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct taccacccag
    16081 cagagaaaag accgctgcag catcggtgga gccgacgata gcgccgatta ataagccttc
    16141 aatcaaatca agattaaaca gccacgccgc catcatgccg gttaaaccag aggtgataag
    16201 tacgcccagc gtcgccagcg acagcgccgg tcctaacgcc acacgaaagg aactggcctg
    16261 agtgcgcatc ccgccgtcga gcaaaataat cgccagcgcc aggttactta ccatgtaggc
    16321 gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg actcccgcca gcatgccgat
    16381 cgccagaaaa ataaccagaa taggaatgcc aagacgggaa gaaaatgaac taagtaatat
    16441 actgctggtg acaaggatgg atcctaagat gaaaaggcta attattgttg tggcatccaa
    16501 tggtcggtta ctcctgatta cgctgtctct tatataaacc ctaccatatt agcggcagag
    16561 acagcgtttt acttagccct gaagcgtgat ttttttacat tttaagaaca ggatgaccgc
    16621 taatcgtcaa ctgagcgcct tcccgggtgt tattcagaat ggcatgtgcg cccaacggca
    16681 gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt aatgagtgga atcgacaggc
    16741 gggaacgcaa aaacgcgtac actgactcca ggttgtaacc tgcgtcataa tcattgggcg
    16801 tgctgccgct aaagctaccg agaataatcg ccttctgacg tggcaaaatt cccgcatgat
    16861 agagctgcaa cagcatacgt tcgacgcgga aagggtgcac gttaatatct tccagcacca
    16921 gaataccgtt ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc agcatcgcaa
    16981 gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg gccttcccct tgccattcaa
    17041 tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag gcgttcagtt
    17101 catccgcacc aaagtttgct accagcatgg ggccgctaaa ggtgatgaca ttgccttgcg
    17161 ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagt aacgggtcat
    17221 gttgttggcg agccaccagt gcctgccagt caatatctgc cagtaaacga ctggcaccgt
    17281 aaccgccgcg tacagccagc acgatggtgt tgggggtagt cagtctggca agggaattga
    17341 gatcttccag acgctccgtt tccgtacccg caaaacgctc acaacgacgg gcaataacct
    17401 cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg gataccacgc agcgcggcgt
    17461 gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa cagagacatg gcaattcctt
    17521 gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt cacctcaacg
    17581 gcgattccag gctataagga tagaagaagt gaaattgaga tggtttgcct ttttgattgt
    17641 gttattagcg ggttgttcat caaagcatga ctatacgaac ccgccgtgga acgcgaaagt
    17701 tccggtgcaa cgtgcgatgc agtggatgcc aataagccag aaagccggtg cagcctgggg
    17761 cgtcgatcca caattgatca cggcgattat cgctatcgaa tcgggtggta atccgaacgc
    17821 ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct ccggacgtga
    17881 tgtttatcgc cgtatgggct ggagtggtga gccgacgacc agcgagctga aaaatccaga
    17941 gcgtaatatt tcaatggggg cggcttacct gaatattctg gaaaccggcc cgctggcagg
    18001 cattgaagat ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta acggggcagg
    18061 tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa tcaacgattt
    18121 ggatgctgac gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc aggctccgcg
    18181 ctatatctac aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg cactttgtcc
    18241 gctttttccc gggcttctcg ctcgagagag aaaataatcc gctgtaattg ccgctccacc
    18301 gtcgggctga cgttaagaaa acggaagctc agacggggag tggtgatggt ttcattcttg
    18361 ccatcaatca ctttgcgctc gctggtggag attaactggg cgtcaaagtg aaaaacaccc
    18421 cattgcccca tgttgacttc aatttgagcg aagcgcatac cttcttgtaa ttcggcaggc
    18481 tttgctgttt ccagtaatgc gcccatgccg cctaacgaca aatcatacaa gcggaaacgt
    18541 aacgtactgt tatccgccag tttggtctgg caaaaataag gcggatggag tggggcggag
    18601 atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac ggtaataaat
    18661 gccggaagct gcaagtattc actctgctgt agttgttcaa cagtaaactc gactttcgca
    18721 ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat gttgtcttcg
    18781 gcttgactgc cgaaatccag caccagttta tccggggtta ttgccagtat tttgctgatc
    18841 agctgcccgc cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa atcgcgtaac
    18901 acgcccagga cggctaacgg attttgtttc aggaactgct catggtaatg actcacgcgg
    18961 aaaaactcct gactgacaaa ctgtctttgg tttatcggtc atagttaaca aaacttaata
    19021 caaatgcgtg aatatttttt acatgttgtt cttaaatcag ccgacaaacg cccagcatcg
    19081 atcccgccct tgcctatact tagagcgttg acgtaagcat ttcttgcgac cgattcatcg
    19141 aaacgagggc ctgaacatgg gaattattgc ctggattatt tttggcttga tagccggcat
    19201 tatcggcaag ctaatcatgc cggggcgtga tggtggtgga tttttcctga cctgtattct
    19261 cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc atgtttggca ttgacggctc
    19321 catcagcggt tttaatttgc acagcttcct ggtggcggtg gtgggcgcta tcctggtgct
    19381 gggcgtattc cgcctcctgc gaagagaata agattttcat aaggcggata gcgatacaga
    19441 tgccgctatc cgctttcaca tcagaacgta tactcgacac ctgcccagaa attgcgacct
    19501 tcttcaataa acccttcact gtaggcatat tgggtatcaa acaggttatt gaccgacgca
    19561 ttgacgctga agccatgacc taaggtgtaa tcggctcgaa tgtgggtcac cgcaaaaccg
    19621 gcggcttttt gtgaaccgtc actgttgctg tagctggagg aacgcgcctc ttccgacagc
    19681 gttacgctta acggctccca tggtttgagt gtcatccatg cggtcattgt ctgcgttgga
    19741 agatcggtta tcttgccgat gtctttgcgt ttggcgtcag cgtggatcag agcgtagctc
    19801 aatcctacat ccagtatatt gctgattttc cccttaatac cggcatccag accgctgtaa
    19861 tccaccgtgc cgctgttctg attttgaatg gtatcggcat tgatattgtg cgagaggatg
    19921 gcatcactca cccggttata gtaaacgctg acctcaaatc cccagtcgcg cgtgaaggca
    19981 ccattccagg ttaaatccac cccgcgagcg cgttccggct tgagctgcgg gttaacaatc
    20041 gctatctggt tatacgcagg tttggatgtg gtatagcgtt ctttcagcgt cggaaagcgt
    20101 gtgcggtcat agtacgaaag cgccagagtg tcttcattgg caaagtggta tttccccatc
    20161 acctgccagt taaaagctga ctgattgttg tcgtcataat gggtgatgct gccatctttc
    20221 tcgtgttttt tcgcttctac gctatcgcgc cagtcatagc tgattcccgc cacgacatcg
    20281 acattatcag cagcagccca ttgatattca ctggcgagcg accaggtacg atcttcatag
    20341 cgatcgtaag cggcgtgcgg cgcacctttt tcccggtgta cgtcatcttt ccagttaacg
    20401 gcaaacgaca gcagatcgtt ttcacgcaca tcggctgcca gttgtaatcc ggcaccgtcg
    20461 ctgtaatcgg aataatggct gtagctgcct tttttatttt tcaaatcagc cagcgagttg
    20521 tacatcatca gcgtattttc aaaggtgtcg cgatacagcc gacttttcag ggtaaaacgg
    20581 tcgtttagtt gggtcgctcc ctgataataa aaactttctt tgtcatactc tggccactgc
    20641 cagtagcgtg atttttgacc acaatttccg ctgtatggcg ggttatcttt ttcaccatcc
    20701 tgcttaatgt aagtcagcgt gtattcatcg ttttcgcgcg gtgtaaaacc gagcttcaca
    20761 atgccgcgtt tatcatcagc cgatgaatta atcatcttgc cgtgtttgcc tgcaatatca
    20821 ttatttacac catgcggcag gccgagaaaa tcctgcttta gctggctacc gctgacttgc
    20881 aaatacccaa gatcgctgct ggcggcaaat gaagcatgca tatcgtaggc attgtcctga
    20941 ctacggctcc atccctggcg atatcccaga cttgcttctt cagggtggtg atattaatgg
    21001 ctccgcccat ctgattaggc ccatgaagca gcgacgaata ccctttggaa acttcgactg
    21061 cccccagatt gttggtcaga atccgcgcca gatcgaggtt gccgtcatag gggacataaa
    21121 tgggcacacc gtcgaaatag accggcacct gccgactatc aaagccacga acttttacct
    21181 gttcttcgtt gcggctgcct gacttttgca gcaccacgcc ggggacgaca cttaatgcct
    21241 gagcgacatt ctgtttatcc aaggccttca tggtgggttg atcgagaacg gtggtcgtcg
    21301 tcgatgatac cggactggac catacggtca gcgtgtcgct ctcctgagcc gctacggcct
    21361 gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt ctcatagaat
    21421 aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac tttaatgtca
    21481 gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca gtcgaggtaa
    21541 tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg aggagccagc
    21601 gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc gagctgaagt
    21661 acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc ttcaacaaca
    21721 attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca gccctgattg
    21781 tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca tccggcacga
    21841 tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt gcccggaatt
    21901 gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa tgccagcatt
    21961 tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac tgccttccat
    22021 ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc cgcgttcgct
    22081 tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa catcttacac
    22141 gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat atagccaggc
    22201 tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc ttccagcagt
    22261 aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc ggtacgctat
    22321 cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca tcttgttgtt
    22381 gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga ctccagctga
    22441 cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt ccacggataa
    22501 aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag atatagttgg
    22561 gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg aacgaggtgc
    22621 tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg ctcattgcat
    22681 ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag cgtggcaggt
    22741 cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc ttcagtgcgg
    22801 cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca taaaccgtag
    22861 tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata tcgaataagg
    22921 tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg tccgttggcg
    22981 aggcgcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc gttttttctg
    23041 tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata tcatcaatga
    23101 gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg tcggcagaaa
    23161 cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag ccttgtgtta
    23221 ctcgtccatc tggctctact tggataatgc tgtcggcaat ggcgttggca tggagcgtat
    23281 ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc gtgtccagaa
    23341 gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac aacaagcgcg
    23401 gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg ctaagcgtat
    23461 tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc gtggctttca
    23521 ggcgttcttg cttaccggga acggaaaaag cgcctatggt gggggccagg cccatcaaca
    23581 ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc caggcaatag
    23641 cctgtgcgcg ctggcgatgg ctgagtgtct gcacagagag gctatccagt aggatctcgc
    23701 cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg ccgcaaccat
    23761 tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg tcgcgtagca
    23821 ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc actggcgacc
    23881 tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca gaatgccaag
    23941 tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga gcagaatagc
    24001 gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca acaagcgggc
    24061 aatgtgcgga acgaccagac cgatccaacc aattatgccc gctattgcta ccgtgctggc
    24121 ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt tcagtcccag
    24181 cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga gaaaaaacag
    24241 gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta aggtcacggt
    24301 ggacagtccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag gatcggccag
    24361 cgttttaatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc ctaccagcac
    24421 cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga cgcccgcgac
    24481 caccatcagc ccgccgcaaa atgcgtacag ctggatgtaa acaatgggta atccccacag
    24541 gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat ccggtgaaac
    24601 taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg ctgcgcccag
    24661 taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt gttcctgcac
    24721 agactcattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga gatgataagc
    24781 cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac atccgtttac
    24841 gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga acaatgccag
    24901 aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc gggatccagc
    24961 caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc atcgagccag
    25021 ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt cacgccctgc
    25081 cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag aataatatcc
    25141 ggctgccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg atcggctatt
    25201 tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga accctgtaag
    25261 cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc ggcgggtgag
    25321 gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg ttctgcggcg
    25381 cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga ttgcgccagt
    25441 tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg tgcctgggag
    25501 atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa cgccataagc
    25561 ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg taactggcga
    25621 atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga aaaaccgacc
    25681 attttttctg gcgcgacagc cagcaagagc aaatcggcgg gcgcgcctgc gctgacgatg
    25741 cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac tgtttgtgaa
    25801 aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa cgtccgtctg
    25861 gttatgggca tggtgaacat cctgaaaagt aaaatcgata tatactttta tatatatcga
    25921 taaaaatcat gttcaatatc aaagtttagc tattgaaaac atgaagatgt gacgacttcc
    25981 gcaagcctgt tgaccctctc tatcattaaa aatgtattta tcaggtggct aatattattt
    26041 aaatcattta aggctaaatt atttaaatat gaatgacatt attgtttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat gataacattc tcc
//
LOCUS       DAJTOW010000052        24999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTOW010000052 DAJTOW010000000
VERSION     DAJTOW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.052"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(40..447)
                     /locus_tag="OO244_002273"
     CDS             complement(40..447)
                     /locus_tag="OO244_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5664077.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(444..1007)
                     /locus_tag="OO244_002274"
     CDS             complement(444..1007)
                     /locus_tag="OO244_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5664078.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(998..1468)
                     /locus_tag="OO244_002275"
                     /pseudo
     CDS             complement(998..1468)
                     /locus_tag="OO244_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(1652..2446)
                     /gene="thyA"
                     /locus_tag="OO244_002276"
     CDS             complement(1652..2446)
                     /gene="thyA"
                     /locus_tag="OO244_002276"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5664079.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(2453..3328)
                     /gene="lgt"
                     /locus_tag="OO244_002277"
     CDS             complement(2453..3328)
                     /gene="lgt"
                     /locus_tag="OO244_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5664080.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(3479..5725)
                     /gene="ptsP"
                     /locus_tag="OO244_002278"
     CDS             complement(3479..5725)
                     /gene="ptsP"
                     /locus_tag="OO244_002278"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5664081.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(5738..6268)
                     /gene="rppH"
                     /locus_tag="OO244_002279"
     CDS             complement(5738..6268)
                     /gene="rppH"
                     /locus_tag="OO244_002279"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5664082.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            6953..7642
                     /gene="mutH"
                     /locus_tag="OO244_002280"
     CDS             6953..7642
                     /gene="mutH"
                     /locus_tag="OO244_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5664083.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            7711..8424
                     /locus_tag="OO244_002281"
     CDS             7711..8424
                     /locus_tag="OO244_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5664084.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            8562..8780
                     /gene="ygdR"
                     /locus_tag="OO244_002282"
     CDS             8562..8780
                     /gene="ygdR"
                     /locus_tag="OO244_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5664085.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8888..9928
                     /locus_tag="OO244_002283"
     CDS             8888..9928
                     /locus_tag="OO244_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5664086.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9961..11151)
                     /gene="lplT"
                     /locus_tag="OO244_002284"
     CDS             complement(9961..11151)
                     /gene="lplT"
                     /locus_tag="OO244_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5664087.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(11144..13303)
                     /gene="aas"
                     /locus_tag="OO244_002285"
     CDS             complement(11144..13303)
                     /gene="aas"
                     /locus_tag="OO244_002285"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5664088.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            13891..14922
                     /gene="galR"
                     /locus_tag="OO244_002286"
     CDS             13891..14922
                     /gene="galR"
                     /locus_tag="OO244_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5664089.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(14929..16191)
                     /gene="lysA"
                     /locus_tag="OO244_002287"
     CDS             complement(14929..16191)
                     /gene="lysA"
                     /locus_tag="OO244_002287"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5664090.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            16313..17248
                     /gene="lysR"
                     /locus_tag="OO244_002288"
     CDS             16313..17248
                     /gene="lysR"
                     /locus_tag="OO244_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5664091.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(17235..17927)
                     /gene="ygeA"
                     /locus_tag="OO244_002289"
     CDS             complement(17235..17927)
                     /gene="ygeA"
                     /locus_tag="OO244_002289"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5664092.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(18056..19474)
                     /gene="araE"
                     /locus_tag="OO244_002290"
     CDS             complement(18056..19474)
                     /gene="araE"
                     /locus_tag="OO244_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5664093.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(19789..20550)
                     /gene="kduD"
                     /locus_tag="OO244_002291"
     CDS             complement(19789..20550)
                     /gene="kduD"
                     /locus_tag="OO244_002291"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5664094.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(20580..21416)
                     /gene="kduI"
                     /locus_tag="OO244_002292"
     CDS             complement(20580..21416)
                     /gene="kduI"
                     /locus_tag="OO244_002292"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5664095.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(21703..22884)
                     /locus_tag="OO244_002293"
     CDS             complement(21703..22884)
                     /locus_tag="OO244_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5664096.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            23139..24368
                     /locus_tag="OO244_002294"
                     /pseudo
     CDS             23139..24368
                     /locus_tag="OO244_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         6442 a         6623 c         5929 g         6005 t
ORIGIN      
        1 caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct gacataatgc
       61 cccctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc
      121 aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaatca ataaggcttc
      181 attatctgcc agtaaacgca aacaaacctg ggcatcggtt ccagcgtact gcgagcactg
      241 aactgctggc tgcgtctgcc aggagtgcat ttttccccac gccagcgccg actgaacgat
      301 ggcctggcgg cgcaatgact gactttccat gctcacgcga gaggcaaaac tgcgatcctg
      361 ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga ccagagccag
      421 tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata gctggcatcc
      481 accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa caccggcgag
      541 aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc atccggatta
      601 gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt ttccagcaca
      661 tgctccttca gacgaaatcc aatctggtcg gactctttta ccggttcgcg atcccagata
      721 ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac aatttccagc
      781 ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt cgcgacggta
      841 aatacccgca gccagatttc atcttccagt gccagcttac gagtgctcgt taaactttca
      901 cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact gatcgccata
      961 gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct tctgtttctc
     1021 cttgctgata aagccggagt cttccccacg gcgaaaccac cagccaccac tcgcccgttg
     1081 agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag aaagcaagcg
     1141 aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac
     1201 tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac gtcccctccc
     1261 tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt aaatagagca
     1321 aatagtcccg cgcctagctg gcggtttgcc aaagccgttg cgactgctgc cagtattgcc
     1381 agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc agcgtttcaa
     1441 tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag aaaaaaagac
     1501 gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt tgcaggacgt
     1561 tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca
     1621 ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct ttaacgcccg
     1681 gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag atggattcgg
     1741 gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat tgcagatgag
     1801 tttgatccat atggttgctg tacaggtgcg tatcgccacc ggtccagaca aaatcaccca
     1861 cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg gcaatgttga
     1921 acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag agtttgccgt
     1981 ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta tccagttcgc
     2041 ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg gtcgtttttc agctggttca
     2101 gtaccgtagt aatctggtca atatgacgac catctggcgt tggccaggcg cgccactgtt
     2161 taccatacac tggcccgagg tcgccatttt catcggccca ttcgtcccag atggtgacat
     2221 tgttttcgtg tagataagca atgttagtat cgccctgcag gaaccacagc agttcgtgga
     2281 tgatggaacg caggtggcaa cgtttggttg tcaccagcgg gaatccatcc tgcaggttaa
     2341 aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg tttttctgtg
     2401 tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa
     2461 cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc gcgacaatca
     2521 tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg gtaaactgcg
     2581 cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca
     2641 agcctgagac agctcccatt gggcgcggtt tacgaatata caggttgagg ataataaaca
     2701 gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg gtggcgcggc agcacaccgt
     2761 aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata tcttctgtac
     2821 gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc cacaattcac
     2881 cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt ggtgcgataa
     2941 aatcagagac ctggaagaag gaacgtttag tacggcgggc gaagataatc atcaccacga
     3001 taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg aacagataca
     3061 gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata cgtcccccga
     3121 ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg gtccagccac
     3181 tgcccggacg attcgcccga cgtgttgcca gccacattgc aaaaatgaaa cccaccagat
     3241 acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc ggatcaaact
     3301 ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa
     3361 agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata
     3421 gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat gatccgcgct
     3481 ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct gcaacctgat
     3541 gacgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt tctgcttcgg
     3601 caaaatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg ttcatagaca
     3661 gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc gccatttcac
     3721 cgcacaaacg aagatcgata ccatgtattt ccgcttcccg ggcgatcatc gccagagctc
     3781 gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac ccgggtattg ttgcgatcaa
     3841 cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg acccgctttg
     3901 ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg atacgtggtt
     3961 tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca atcaggcggc
     4021 gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa cagaatattc aggttgcccg
     4081 tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc ggctgatcga
     4141 gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc ggcatgtaag
     4201 gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta tcattgaaca
     4261 tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa ccactttgca
     4321 gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacagctg cccagttttt
     4381 cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca ccgcttttta
     4441 actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc tcttcactaa
     4501 ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat tcaccgcgat
     4561 agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc gcgcccatca
     4621 cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct gcgccatctc
     4681 gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc gctgacagtt
     4741 catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa
     4801 gatgaaacag caatcgctga cccagcgcac gtaaatcacc agcccgctct ttgagatagt
     4861 tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt accgcccact
     4921 ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa
     4981 gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg gcggcaaagc
     5041 gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc agtcgttcgc
     5101 gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa ggtaacgtgg
     5161 catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg cggattcgcg
     5221 tctggcgata ttgcccaaac agggcagtca actgcgactg agaaagaata gctgccatct
     5281 gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc aactctcgtt
     5341 gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg cctaaaaacg
     5401 cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc ttttgcgcat
     5461 ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct tcatcaaacg
     5521 cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc aggtagtaac
     5581 aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc gcaagacaga
     5641 tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct accttttcga
     5701 ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta cgtcgataag
     5761 cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca ctcgcgaact
     5821 ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg accggatacc
     5881 agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc atattgattt
     5941 ctgcatcgcc gcttaccagc tgcaagagaa accatttttg tttttggccg atacaaaccg
     6001 gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg
     6061 aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat tcacggtaca
     6121 tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc caggagtgct
     6181 gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg atacctacgt
     6241 ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct ttatatacga
     6301 atgattgttt catactccag gaagacggta aaccactctc tgcagggcat tacacactaa
     6361 taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg ggtggatagc
     6421 cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt actacgggaa
     6481 aacccggagc attctgaata agcccgatat agacatttta aaatattcat tattagtcaa
     6541 tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc
     6601 atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat ccacagaatg tgccactaag
     6661 ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatgaaaac
     6721 agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct
     6781 gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac tcgattaccg
     6841 gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa aagttacact
     6901 gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg acatgtccca
     6961 acctcgccca ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca
     7021 actttctggt tatacattgg gagaactggc ggcacttgcc gggctggtta cgccagagaa
     7081 tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc
     7141 agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt
     7201 ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgcccggt taacgggtaa
     7261 tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg tactgtggat
     7321 accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct
     7381 gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga
     7441 tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggaat atttacagat
     7501 aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg
     7561 gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cactactggc
     7621 ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta
     7681 ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac cgatcctaac
     7741 gcttggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt
     7801 attttccttt ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt
     7861 ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat ctcttgggtt
     7921 acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttctgc ccgtgatttg
     7981 attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc
     8041 attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg
     8101 cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg
     8161 tcagatcacc tgtttattat gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc
     8221 gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc
     8281 tctttcctga ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg
     8341 aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacttgatt
     8401 cgcaacaaaa agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac
     8461 tccttttacc ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata
     8521 aacagactat tatcataggt aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa
     8581 taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg
     8641 cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg
     8701 ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt
     8761 cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc
     8821 tttccctttt cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga
     8881 aacggctatg caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct
     8941 tggcacgatg acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta
     9001 tgccgtcgct cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg
     9061 ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag
     9121 ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg
     9181 catccgcccg gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct
     9241 caagcgccta cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccgac
     9301 caactgcttc ggcaaactcg gttatagctg gacggattct gcgcctgcgg tttcgctgct
     9361 ggatacgctg gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt
     9421 gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct
     9481 gccgcgtatt gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg
     9541 tctggcagaa gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt
     9601 cggcacgctg accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct
     9661 ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt
     9721 tgatatcgcc agacgtcatg gccttgatcc tgctcagatg gcgctcgcgt ttgtacgccg
     9781 tcaaccgttt gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa
     9841 catcgaaagt ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtaca
     9901 tcaggtttat acttatcctg caccataaag attaacgccg gttaaaaccg gcgttaaata
     9961 ttaatgacgg cgctgccaga tccacagcgc cgttattgcc agcgcaaata gcgcaccgaa
    10021 gccaatgcca atgggcacga ccgggatgcc taccattacc gccagcgagt aaatgcccag
    10081 catcaacaac atggcgctgt tttcgccaag gttttgtact gcaatcgcat tccccgcccc
    10141 gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc
    10201 cagcacgcca atcagcatca acaaggcata ggctggcagc agctcgtgtt gcagggagaa
    10261 aatcggtacc accacgccaa tcaaaatccc ggctggcata cagcgtgaca cggtttccag
    10321 cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt
    10381 gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc acagcaccaa
    10441 caggaaacgc agcgtgacac ccgctcccca gaataaactg gtgcccacca gcgaaaaacg
    10501 cgtttcacca ttgcaccata gcgaggtgca ggcattcagg aaactgcggg tcatgttgat
    10561 gagattccag gactgccccg gacgcgccgc cagtttggga atgtagatat tggcaacgac
    10621 cgcaccaccg taggccagtg cgcatgcggc cagagcgacc aggacatgcc agtcagccag
    10681 cacaccaccg gctacggaac cgagtaaaat cgccgctatg gcagaagctt ccattaaacc
    10741 gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    10801 tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa
    10861 gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt
    10921 ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa agaatgtaag cacctacaaa
    10981 caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag
    11041 agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    11101 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    11161 ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    11221 gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc
    11281 gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    11341 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    11401 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    11461 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag
    11521 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    11581 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    11641 gtccgccctc ttcgatacca gggaccgaca acaggcgtgc atccattcct ggtagaatac
    11701 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt
    11761 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg
    11821 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg
    11881 ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac
    11941 tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca
    12001 cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    12061 gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    12121 cgacgttcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg ccttcagaac
    12181 cggaggtaaa gaggatcagc gcctcttctt ccggctgctg ttttacctgc gccaggcgcg
    12241 gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat
    12301 cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt
    12361 tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg
    12421 tcagcccttt taccccggca gtgtagttca tcattgcggg aatgcggcga cgcgcgatgg
    12481 ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc
    12541 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    12601 gataggagtc tggggtaaag ttgacgtctt cgacacattt cttcccggct ccgaagcggt
    12661 acattgcact cagtaaagat tcgtacagag tttcacgcgg gcgcaccgcc atacgcgctt
    12721 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    12781 catccggcat cgccacctgc gttggaggca aaatatgcag agtaatttgc gggaacaagc
    12841 gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    12901 caggaataac cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg tagattttca
    12961 tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    13021 ccaccagacg taccagatgt ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt
    13081 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    13141 acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat
    13201 taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc
    13261 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    13321 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    13381 attaggtgcg aaaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg
    13441 aagagcgtac cgaataatct caccaatcaa tacctctggg atcttgattg tggtctgcac
    13501 gacgactctt cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta
    13561 aattgtcggt tactgttaca gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag
    13621 gctggtgtaa ttcatgtgct caacacgaag ttgacttcac ctatcaatac ctctgggatc
    13681 accactttag caacctgaag ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa
    13741 agtgtaacca aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat
    13801 tcccacgatg aaaacacgcc accccttgaa ccaacgggcg ttttccgtaa cactgaaaca
    13861 atgtaagcgt ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg
    13921 gcaggcgttt cagtcgccac cgtttcccgc gtcattaata attcacccaa agccagcgaa
    13981 gcttcccggc tggctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac
    14041 gcccgtgcgc tggcgcagca gaccactgaa acggtcggtc tgatcgttgg tgatgtttcc
    14101 gatccgtttt tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca caccggtaat
    14161 ttcttattga ttggcaacgg ttaccacaac gaacaaaaag agcgtcaggc tattgagcaa
    14221 ctgatccgcc atcgctgtgc tgcgttggtc gtccatgcca aaatgatccc agatgccgat
    14281 ttagcctcat taatgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc
    14341 tttgaaaacc gttgtattgc tcttgacgat cgttacggtg cctggctggc aacgcgtcat
    14401 ttaattcagc aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac
    14461 gccgaagatc gtctgcaagg gtattacgat gcccttgctg aaagtggtat tccggccaat
    14521 gatcggctgg tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag
    14581 cttttgggac gaggaagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg
    14641 ggcgcgatgg gcgttctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt
    14701 ggctttgatg atgtgctggt gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac
    14761 ccaatcgtaa cgatggcgac ccaggctgcc gaactggctt tggcactggc ggataatcgc
    14821 cctctcccgg aaattactaa tgtatttagt ccgacgctgg tacgtcgtca ttcagtgtca
    14881 actccgtcgc tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc
    14941 cagcgccagt aattcttcga tggtctggcg acggcgaatc aaccgcgcct gaccattatc
    15001 aaacagaact tctggtaaca gcggacggct attgtagttg gatgacattg atgcgccata
    15061 tgcccctgta tcatgcagta ccagataatc acctgccttc acttccggca aggcgcgggt
    15121 ttcaacattt cccccttcct gctgggtaaa gacatcgccc gattcacata acggcccggc
    15181 gacgacggtt tccaccgttg gcgcgtgttc cagagaacga ccatcagctg ccagggcact
    15241 gatatggtgg taactaccgt acattgccgg gcgcatcaga tcgttgaacc cggcatcaac
    15301 cagcacaaag tggcggctac ccatttgttt gacgctccgc acctgagtaa ttaatacgcc
    15361 agactgcgct accaggaagc gacccggttc aatttccagt ttcacagggt ggcccaaatg
    15421 gcgagcgatt tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc
    15481 ctcttcaccc tgttgataag gaatagaaag cccaccgccc gcagaaatag cctgtaaatc
    15541 ctgaccgaat tcgataacct gacgcaccat agcaccacac acctgctcca gatgggcata
    15601 atcaacgcca gaaccaatgt gcatgtgaat gccgactagc tgcagatgat gacgttgtat
    15661 cacgtccagt gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc
    15721 ggtattggtt ttttggctat gtccgtgacc aaaccccgga ttaacgcgca gccatacccg
    15781 atgccctggc gaaacctggc ccagttggtc gagcgtatca acagaacccg cattcaccgg
    15841 aatttgcaat tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac
    15901 aatatcatcg gggtgcgttt gcggattgta acccgccgcc aacgcacgct ctatttcgcc
    15961 taacgagacg gaatccactt tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt
    16021 ggaacaggct ttctgtgcaa agcgcaccac ttcaaactgt ttcagcgctg caatctgccg
    16081 acgaataatt tgcgcatcgt agacccacac cgggcagcca aattcagcgg gcaaacgcag
    16141 cagattttcg gcggtgagat cggtatcggt gctgaacagt gaatgtggca taacaaactc
    16201 cagatgagtg cttttttatg attacgccac atcataaaaa gaataaaaaa tatcgattta
    16261 tgtcgagtct atgcaaaaat gatatggatt accggattgc gagagagcgc taatggccgc
    16321 cgttaactta cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga
    16381 ggcagcacac ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga
    16441 gaaggtgatc gggctgaaat tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca
    16501 aggactgcgt ctgtttgaag aagtgcaacg atcctggtac ggactggatc gcattgtcag
    16561 cgccgcagaa agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt
    16621 cttttcgcaa tcttttttac cgcagctcct gcaacccttt ctggcacgtt atcccgatgt
    16681 cagcttaaat atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg
    16741 tcatgattta ggactcactg agacactcca tacgcctacg ggaacagaac gtaccgaatt
    16801 actctcttta gatgaagtgt gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt
    16861 attaacgccg gatgattttc agggtgagaa ttacatcagc ctttcccgta ctgacagcta
    16921 tcgccagttg ctggatcagc tatttactga acatcaggtt aaacgacgca tgatcgtaga
    16981 aacccacagc gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggta tttcggtggt
    17041 taacccgctc accgcactag attatgcggc aagcggttta gtggtgcggc ggttcagtat
    17101 tgcggttccg ttcaccgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt
    17161 tcaggcgttt agtgggcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc
    17221 tattttgtcg tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgcg
    17281 gtatcaaaca caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag
    17341 ccaaaaatga cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa
    17401 gcgcgtgacg cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt
    17461 ttcgcccgtt catccgcttc aggaataaga caattgatgg aaaattgttc cgtcagccgc
    17521 ccgcgataaa aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt
    17581 ccggccccgg taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa
    17641 cgtgactcaa tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct
    17701 gcgcccgccc gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat
    17761 tccccgcgac gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcatttgc
    17821 gcagagtgaa gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga
    17881 atggtggatt cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga
    17941 tgtgtgttac cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc
    18001 ccaatctcta ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga
    18061 cgccgatatt tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga
    18121 catttttggt ttccggaatg agccagaaag taatacccac aaacgcaatg ttcagcgcag
    18181 tgtagagcca gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggccgcgc
    18241 cgataatcat attcgacacc cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc
    18301 atttcagcgg ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat
    18361 aaccggcaat acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg
    18421 taccgttatc aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc
    18481 tgaaaccaat tttcagagcc ggtttacgac ctgctttatc taccgtaaag accgcaatga
    18541 aggtcgcgaa cataaaggtc agtcccacga ccagagtcgc aatcatctgt tgttctgtgg
    18601 tcgtaaagcc cgccattttg aagatacgcg gcgcgtagta catgatgatg ttcataccgg
    18661 taaactgctg catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt
    18721 tgatcttaaa cagtgcccaa ccgccctgtt ttaacttcag gctttcacga atttcgttga
    18781 gttcttctcg cgctttttcc gacgtatcgc gcagcatacg caacacttct tccgcctcaa
    18841 tatgacgccc cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa
    18901 tcagcagaac tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac
    18961 tgaacgctgt atcggataaa aacgccagca cgatgccgag tgtgaccatc aactggtaca
    19021 tactgatcat cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg
    19081 tgtaagacgc gatcccgaca gcaatgccca gcaccacacg agcggcgatt aacatctcta
    19141 cgctggtcgc aaaagcggac cctatagaac cgagtacaaa caggatggcc cccgccatca
    19201 ggctgtattt acgccccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg
    19261 cgccgagcat catgctacta accacccatt cctgcaaacg actggtcagc acaaagtgat
    19321 cggtaatgaa cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg
    19381 cgaccgcagc agctaccgaa acaaacatat tcatacgccg cgtatcacgc aaagaacgtg
    19441 gcgttaaagc agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac
    19501 attgtgaaaa aatacgtgaa caattcacac aggtgtcagg tcggaaacag cataaatatg
    19561 gattaaattg ctgcgacatg ccgttatgtg atggatattc caattttaaa attaagttga
    19621 attattgaga ttattattaa cctcctaatt ttaaagcaga taaaattcat aaagttcatt
    19681 aattgataat tgatatggat tatttcataa ccatgatatg gattatgatg atctagagat
    19741 ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga ggaatgaatt aacgcgccag
    19801 ccaaccgcca tccacagcaa tggtataacc atttacataa tctgaagcgc tggaggcaag
    19861 aaacactacc ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcgatc
    19921 gagaatttcc gcgctacgtt gttcatctgc ccgcagttgt tgggtattgt tggtcgccat
    19981 gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcgt ttgccatcaa
    20041 tcgcgtcaca cccatcacgc cgctttttga tgcggtataa gaaggcacac ggatcccgcc
    20101 ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa
    20161 gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc
    20221 gtcccagtct ttttcgctga actcgagagc gtcttcgcgg cgaatcaatc cggcgttatt
    20281 caccaggata tcaatatgac caaattcagc taccgcgcga tccagcagtc caggaatgcc
    20341 atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg
    20401 ctcgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc
    20461 cagccccaac gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt
    20521 accttggaga gaaaatgcac ttaaaatcat aacaacacct tattcgttta tgcccacaac
    20581 tagcgcaaat ctttaacgcc cacatggtcc atatcattaa agacctggtt ttcaccgacc
    20641 atgccccaga taaaggtata agctttggtt ccgacgccgg aatggatcga ccagctcggg
    20701 gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc
    20761 atgtggaaaa cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc
    20821 tcgtgggtgt gacacggcat ggtgttccac aagttacctg gagccagctc cgtcagcccc
    20881 atactcaatt gacaggtttc cagtacatcc gggacaaaat atttgttaat cgtgcgacgg
    20941 ttactggtga ggttatcgcc taacgtgact ggagatactt cgtccggtgt gacttttttg
    21001 gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa acttcgccgg agtggcggta
    21061 tcaatactgg caaagacaac ttcttttgca cctttaccaa catacagggc gtcgcggtga
    21121 ccgatttcat agcattgacc atcgacagta atcgtaccgg caccgccaat attgataaca
    21181 cctaactcgc gacgttcaag gaaatagctt acgccgagtt gtttaccaac ttccccgcca
    21241 acggaaaccg ttttagttat cggcataatg ccgccaacaa taattcggtc aatgtggctg
    21301 taaaccatgg tgtactcatc ggcgacaaat accttttcaa ccaaaaattc attgcgcagc
    21361 ccttgggtat ccagcgtttt tgcgtgcgca ccgtggatgc tctgtcttac gtccacatca
    21421 acctccgata atcagtgaag cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt
    21481 ctctataaag ggatgatatg cccatgggct agggttttca attaaaaata aaacaatgtt
    21541 ttatttttaa tttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat
    21601 ggaaaaatga gggagcccag aaaatgtatg tcgacttgcc agccatcatt attcaacaga
    21661 ttaatgacgg cagatattta tcggtaagga ggaaatgcag agctactcgt cacgttcaat
    21721 ggtcaatgcc acgccctggc ccccgccgat acaaagcgtt gccagccctt tgcgggcatt
    21781 acgtttcacc atttcatgaa ccagagaaac caggattcgg caaccggaag cgcctatcgg
    21841 gtgaccgagt gcgatcgcgc caccattgac attgacccga cgctcatccc actcaagcat
    21901 cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc
    21961 agccaactgc cagcctacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc
    22021 catcaatgcc ggatctacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa
    22081 attcaacgct cgtgctttgg cttcgctcat catcattacc gcagctgcgc catcgtttat
    22141 ggatgatgca ttccccgcgg tcacagaacc gagactatca aatgaaggat ttaaacgggc
    22201 taaaccttct gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg
    22261 accgttactt tggatcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc
    22321 cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc
    22381 atattcgcga gccagatttt cggcggtgac accaatatga taatcattga aggcatccca
    22441 caacccatca tgcaccagac tgtcaaccaa ctggctattg ccaagctgtt caccggtgcg
    22501 gctatcagtc agaacatgtg gtgcgcggct catgttttcc tggccaccgg cgatgacaat
    22561 atcagcctcg ccacactgta tcgcctgagt agccagatgc agtgctttaa gaccggaacc
    22621 gcaaacgtca ttaatcgtga ttgcagaaac gctgttagga agaccacctt taatagccga
    22681 ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa ttacttcatc
    22741 caccgcatat gcaggaacgc cggtacgttc tattaacgct ttcacgacca gactaccaag
    22801 ttccacggcg gaatgacccg ctaacgcacc acgaaagcag ccgataggtg tccgtaacgc
    22861 cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat
    22921 gattgttaat tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat
    22981 atgcctgagc agtagtcaga gtcataactg gcacgtaagg tttgcaacca ctaacccacc
    23041 aatagatggg tagatagggc gttaatctcc catacttaac ttggtttatg gtaaattgcc
    23101 ctccattttg tttaatttgt agatgatacg ttcagataat gtctaatatt tggtctaaag
    23161 aagaaactct gtgaagtttc gcgctctatg gcacagccgt tgatgcaggc acgctattcc
    23221 ttcctattca gttaggttcg gcaggggctg tggtcctgtt tattactgct ctggtcgcct
    23281 ggcctttaac atattggcca cataaagcct tatgccagtt catcctctca tcaaaaacat
    23341 cagcaggtga agggataacg ggcgcggtaa cacactacta tggcaagaag attggtaatc
    23401 tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa
    23461 ttaccaactc acttacggaa cagctggcaa agcatatggt tattgatctt cgcatccgta
    23521 tgttggtgag tctgggtgtt gtattaattc tgaatctcat ttttctgatg ggacgtcatg
    23581 ccactattcg ggtaatggga tttttggtat tcccattgat tgcctatttc ttatttcttt
    23641 ccatttacct tgtcggtagt tggcaacctg atctattaac aacccaggta gagttcaatc
    23701 agaataccct tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata
    23761 cgcccattat ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg
    23821 ataaatgcaa aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct
    23881 ttgtctttag ctgcctgctt tctattccat cttcgtatat tgaagcggct aaagaagaag
    23941 gggtcaccat tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ttgtcaattt
    24001 ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg
    24061 ttattgaagg tgccacagag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa
    24121 gtcgtgcatt taaccacgca ctatcaatta tgttggtatc gctgattacc ttcattgttt
    24181 gttgcattaa cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca
    24241 tgatactttt catcatgcct acgctgtcaa cgtatctcat cccggcgctt aaaccctggc
    24301 gttccatcgg aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct
    24361 ttagctaata tccgttgtgg atggttcaaa attgctacaa ttttgaacca ttccattttt
    24421 cataatattc atttgttatt attccgccct atcacttctc tcgtcatatc ctagaattat
    24481 cagttagtta aacacacaaa gagatctgaa catcttaaat aagactattt aagatgcata
    24541 acttagattc gcaagatatc ttctggtcat tcaaaaacaa tttgcgatat agatattctc
    24601 ataccatgag atagttatct ggaccacgtt cgaccatgca agattaaatc agtgaaacat
    24661 aatcatattt aataacgaga aatgcatttt taaatatctt aatcagaatt atagcaaata
    24721 cagattaatc gcaatttttc ccagcaaaat tacaaaatca aggtgtattg acatcatcaa
    24781 tcaccaatga aattatgcaa ttatatacgg atagggaggt tcttaacatg gggctttgta
    24841 gtcgttataa aagtcttaca tgcaatagtt gctcaatgca ttgccaaata atgccagaag
    24901 agtcaccgcg tttgcagtac tgcgcaaact cgtgttttga tcttacccag caatagtgga
    24961 cacgcggcta agtgagtaaa ctctcagtca gaggtgact
//
LOCUS       DAJTOW010000053        24965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTOW010000053 DAJTOW010000000
VERSION     DAJTOW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.053"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..465
                     /locus_tag="OO244_002295"
                     /pseudo
     CDS             1..465
                     /locus_tag="OO244_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            793..1875
                     /locus_tag="OO244_002296"
     CDS             793..1875
                     /locus_tag="OO244_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5664097.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2364..3162)
                     /locus_tag="OO244_002297"
                     /pseudo
     CDS             complement(2364..3162)
                     /locus_tag="OO244_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3172..3723)
                     /gene="ybcL"
                     /locus_tag="OO244_002298"
     CDS             complement(3172..3723)
                     /gene="ybcL"
                     /locus_tag="OO244_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5664098.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3892..4224)
                     /gene="emrE"
                     /locus_tag="OO244_002299"
     CDS             complement(3892..4224)
                     /gene="emrE"
                     /locus_tag="OO244_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5664099.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4569..4883)
                     /gene="fliE"
                     /locus_tag="OO244_002300"
     CDS             complement(4569..4883)
                     /gene="fliE"
                     /locus_tag="OO244_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5664100.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5097..6756
                     /gene="fliF"
                     /locus_tag="OO244_002301"
                     /pseudo
     CDS             5097..6756
                     /gene="fliF"
                     /locus_tag="OO244_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6749..7744
                     /gene="fliG"
                     /locus_tag="OO244_002302"
     CDS             6749..7744
                     /gene="fliG"
                     /locus_tag="OO244_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5664101.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7737..8423
                     /gene="fliH"
                     /locus_tag="OO244_002303"
     CDS             7737..8423
                     /gene="fliH"
                     /locus_tag="OO244_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5664102.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8423..9796
                     /gene="fliI"
                     /locus_tag="OO244_002304"
     CDS             8423..9796
                     /gene="fliI"
                     /locus_tag="OO244_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5664103.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRIGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9818..10159
                     /gene="fliJ"
                     /locus_tag="OO244_002305"
                     /pseudo
     CDS             9818..10159
                     /gene="fliJ"
                     /locus_tag="OO244_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10156..11283
                     /gene="fliK"
                     /locus_tag="OO244_002306"
     CDS             10156..11283
                     /gene="fliK"
                     /locus_tag="OO244_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5664104.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11388..11852
                     /gene="fliL"
                     /locus_tag="OO244_002307"
     CDS             11388..11852
                     /gene="fliL"
                     /locus_tag="OO244_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5664105.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11857..12861
                     /gene="fliM"
                     /locus_tag="OO244_002308"
     CDS             11857..12861
                     /gene="fliM"
                     /locus_tag="OO244_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5664106.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNNIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12858..13271
                     /gene="fliN"
                     /locus_tag="OO244_002309"
     CDS             12858..13271
                     /gene="fliN"
                     /locus_tag="OO244_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5664107.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13274..13639
                     /gene="fliO"
                     /locus_tag="OO244_002310"
     CDS             13274..13639
                     /gene="fliO"
                     /locus_tag="OO244_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5664108.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13639..14376
                     /gene="fliP"
                     /locus_tag="OO244_002311"
     CDS             13639..14376
                     /gene="fliP"
                     /locus_tag="OO244_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5664109.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            14386..14655
                     /gene="fliQ"
                     /locus_tag="OO244_002312"
     CDS             14386..14655
                     /gene="fliQ"
                     /locus_tag="OO244_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5664110.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14664..15449
                     /gene="fliR"
                     /locus_tag="OO244_002313"
     CDS             14664..15449
                     /gene="fliR"
                     /locus_tag="OO244_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5664111.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15734..16357
                     /gene="rcsA"
                     /locus_tag="OO244_002314"
     CDS             15734..16357
                     /gene="rcsA"
                     /locus_tag="OO244_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5664112.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16401..16589)
                     /gene="dsrB"
                     /locus_tag="OO244_002315"
     CDS             complement(16401..16589)
                     /gene="dsrB"
                     /locus_tag="OO244_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5664113.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16752..16979
                     /gene="yodD"
                     /locus_tag="OO244_002316"
     CDS             16752..16979
                     /gene="yodD"
                     /locus_tag="OO244_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5664114.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17277..18092
                     /locus_tag="OO244_002317"
                     /pseudo
     CDS             17277..18092
                     /locus_tag="OO244_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18089..19783)
                     /gene="yedQ"
                     /locus_tag="OO244_002318"
                     /pseudo
     CDS             complement(18089..19783)
                     /gene="yedQ"
                     /locus_tag="OO244_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19954..20136)
                     /locus_tag="OO244_002319"
     CDS             complement(19954..20136)
                     /locus_tag="OO244_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5664115.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20215..21132)
                     /locus_tag="OO244_002320"
     CDS             complement(20215..21132)
                     /locus_tag="OO244_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5664116.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21305..22225
                     /gene="yedA"
                     /locus_tag="OO244_002321"
     CDS             21305..22225
                     /gene="yedA"
                     /locus_tag="OO244_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5664117.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22214..22684)
                     /gene="vsr"
                     /locus_tag="OO244_002322"
     CDS             complement(22214..22684)
                     /gene="vsr"
                     /locus_tag="OO244_002322"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5664118.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22665..24083)
                     /gene="dcm"
                     /locus_tag="OO244_002323"
     CDS             complement(22665..24083)
                     /gene="dcm"
                     /locus_tag="OO244_002323"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5664119.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24150..24690)
                     /locus_tag="OO244_002324"
                     /pseudo
     CDS             complement(24150..24690)
                     /locus_tag="OO244_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24730..>24965)
                     /locus_tag="OO244_002325"
     CDS             complement(24730..>24965)
                     /locus_tag="OO244_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664120.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6307 a         6406 c         6154 g         6098 t
ORIGIN      
        1 tgttaattaa ctatcccccc aaactggcga tatccagtct ggtaaacaaa cagcctcaaa
       61 ggcgtatcgg gtaggttact gcgacgggat cgaccagata ttgctgccag gtattaccgc
      121 aaagatgttt tgtggcgtcc cggctatttt gccagtagct gcggaggtgc gccaatatcc
      181 gtcatccgcc aatacattga acaacagcaa acacctggtc agatgaaaaa acgagcctta
      241 tatccccgcc ctgaaggacg ggcttttacg gtgcactgga taagcctgga gattatctac
      301 acctgcatgg acatcattgt actatgcccc gtacatgctc catcacatcc tcaacatcaa
      361 agtattagag ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa
      421 aaaatagaac taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac
      481 attaataaat ctaaataaat atcatgcaaa taatttccat ggttattttt aggctattta
      541 aattaaaaaa ataaattatg agtcaatcat aaataacaac ctttaatata atttgactac
      601 caaatttata ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata
      661 aaaataggga tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc
      721 tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata
      781 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg
      841 atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg
      901 tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca
      961 acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc
     1021 ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat
     1081 aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc
     1141 cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt
     1201 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc
     1261 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag
     1321 ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact
     1381 acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat
     1441 gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg
     1501 gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc
     1561 cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta
     1621 gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
     1681 ggaaaagact tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca
     1741 acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat
     1801 aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg
     1861 gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac
     1921 atataagtgg cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata
     1981 aattaccctt tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca
     2041 attaacgtat caattagatt taataacaac gatatccgta cctaacctga tatctaatgt
     2101 cataagcatc cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca
     2161 catagcccaa acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag
     2221 gaagtgagta tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga
     2281 ttgttcaggc tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc
     2341 tgttgctcct taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt
     2401 atattaaaac gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc
     2461 ctggcaatct cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa
     2521 cctcacgtcg tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa
     2581 tgtggaaaca gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttctagg
     2641 ttcggatagc attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag
     2701 taaacaatta acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg
     2761 aatttgcgtt gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa
     2821 ctcggggttc tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata
     2881 aaatttttga aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag
     2941 acctgcttct ggataattca caatataaaa ttcatatcca cagggaaata atattatctg
     3001 attattatca acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat
     3061 acgacataaa gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg
     3121 tatttcttca atctttaggt tagacttatc tcttgccagc atactcttac cctattttat
     3181 ctcataaact ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac
     3241 taacgctccg ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt
     3301 aaactggtaa tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata
     3361 accaaaatca tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc
     3421 tgcatcagtg ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca
     3481 tccactgcct gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc
     3541 aggagcacca gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga
     3601 aaatacatga gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa
     3661 tgctgcggcc tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt
     3721 cataaaaacc tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta
     3781 tgcataaagg cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt
     3841 agccataata tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg
     3901 tgcacttcgt gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat
     3961 gatagctggc aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac
     4021 gataccgact cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg
     4081 agctaataac cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg
     4141 tgtaaacccc tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc
     4201 accaagataa atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt
     4261 actacagttt gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct
     4321 ggcgtggcaa gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa
     4381 tgagttcact cttcctggga gcaaagcgaa gacaaaacat gttatcgcat ttacggatcg
     4441 tggatggcag aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt
     4501 gccccatcca tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt
     4561 tacacaacct acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc
     4621 acctgaatcc ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt
     4681 agcgccacgc cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt
     4741 gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc
     4801 ggcagtgatt cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct
     4861 tcaatcccct gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac
     4921 cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat
     4981 agaaaaaacg aaagcacaaa taatgggagc gtcaattttt cgagtgtgct gacccgagag
     5041 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgaag tgcgcgatga
     5101 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
     5161 aaattccatt gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
     5221 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
     5281 caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg
     5341 ctattgaggt tccggcagat aaagttcacg aactgcgcct ggcgcaacaa ggtttgccca
     5401 aaggcggcgc ggtcggtttc gaactgctcg atcaggaaaa gtttggtatc agccagttca
     5461 gcgaacaggt gaattatcag cgggcgctgg aaggcgagct ttctcgtacc atcgaaacta
     5521 tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc gaaaccgtct ttattcgtcc
     5581 gtgaacaaaa atccccttct gcatcggtga cggtaaatct gttacccggc cgcgcactcg
     5641 atgaagggca aattagcgct attgtacatc tggtttccag cgcggttgct ggtctgccgc
     5701 ctggaaacgt cacgctggtg gatcagggcg ggcatctgtt aacccagtcc aataccagcg
     5761 ggcgcgatct taatgacgct cagttgaaat atgccagcga tgtcgaaggc cgtattcagc
     5821 agcgtattga agcgatcctg tcacctattg ttggtaacgg taatattcac gcctaggtca
     5881 cggcgcaact ggacttcgcc agtaaagaac aaacggaaga acagtatcgc cctaacggtg
     5941 atgaatctca tgcggcgctt cgttcacgcc agcttaatga gagcgagcaa agcggttccg
     6001 gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc ggcacctgcg aataacgcgc
     6061 caatcagcac gcctccgaca aatcaaaata accgccagca gcaggcgagc accaccagca
     6121 atagtgggcc gcgtagcaca cagcggaatg aaaccagtaa ctacgaagtc gatcgcacca
     6181 ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct gtcagtcgcg gtagtggtga
     6241 attacaaaac cttgccagac ggtaaaccat tgcctctcag caacgaacag atgaagcaaa
     6301 ttgaagctct gacccgcgag gcgatgggct tttctgaaaa acgcggtgat tcgctcaatg
     6361 ttgttaactc gccgttcaat agcagtgacg aaagcggcgg agcactgccg ttttggcaac
     6421 agcaagtgtt tatcgatcag ttgctggctg ccggtcgctg gttgctggtt gctggtactg
     6481 ctggtggcgt ggttgctgtg gcggaaagcg gtacgcccgc agctaacacg tcgcgccgag
     6541 gcggtgaaga ctgtgcagca acaggcgcag gcccgcgagg aagtggaaga tgcggtggaa
     6601 gtccgtctga gcaaagacga acaactccaa caacggcgcg ctaaccaacg actgggggca
     6661 gaagtcatga gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg
     6721 gtcattcgcc agtggataaa taacgatcat gagtaacctg acaggcaccg ataaaagcgt
     6781 catcctgctg atgaccattg gcgaagaccg ggcggcagag gtgttcaagc acctctccca
     6841 gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca
     6901 gctaaccgat gtgctggcgg agtttgagca agaagctgaa cagtttgctg cactgaatat
     6961 caacgccaac gattatctgc gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc
     7021 cagcctgctg gaagatattc tcgaaacccg cgataccgcc agcggtattg aaacgctcaa
     7081 ctttatggag ccgcagagcg ccgccgatct gattcgcgat gagcatccgc aaattatcgc
     7141 caccattctg gtgcatctga ggcgcgccca ggccgccgat attctggcgt tgttcgatga
     7201 acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc
     7261 gctggccgaa ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag
     7321 caaaatgggc ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa cgcagcagga
     7381 agaagccgtt attaccgccg tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga
     7441 cgagatgttc ctgttcgaga atctggtgga tgtcgatgat cgcagcattc agcgtctgtt
     7501 gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa ggggccgagc agccactgcg
     7561 cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc
     7621 caagcgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa cagaaagcga ttctgctgat
     7681 tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc agcggcgagg atacctatgt
     7741 ctgataatct gccgtggaaa acctggacgc cggacgatct cgcgccacca ccggcagagt
     7801 ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga ggccgaaccc agccttgagc
     7861 agcaactggc gcaactgcaa atgcaggccc acgagcaggg ttatcaggcg gggattgccg
     7921 aaggtcgcca gcaaggtcac gaacagggct atcaggaagg actggcccaa gggctggaac
     7981 aaggtctggc agaggcgaag gctcaacaag cgccaattca tgcccggatg cagcaactgg
     8041 tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt gatagcttcg cgcctgatgc
     8101 agatggcgct ggaggcagca cgtcaggtga tcggtcagac accaacggtg gataactcgg
     8161 cactgatcaa acagatccaa cagttgttgc agcaagaacc gttattcagc gggaaaccac
     8221 agctgcgcgt acacccggat gatctgcaac gtgtggatga tatgctcggc gcaactttaa
     8281 gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca ccctggcgga tgtaaagtct
     8341 ccgccgatga aggcgatctc gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc
     8401 tggcagcacc aggagtggtg taatgaccac gcgcctgact cgctggctaa ccacgctgga
     8461 taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc gattaacccg
     8521 cgctaccggg ctggtgctgg aagccaccgg attacaattg ccgctcggcg caacctgtgt
     8581 cattgagcgc cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa
     8641 cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa ggtgtcctgc ccggcgcgcg
     8701 tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc ggcaagcagt tgccgctcgg
     8761 tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa ccgctcgatg gcctgccctt
     8821 ccccgatacg acggaaaccg gtgcgctgat taccccgcca tttaacccgt tgcaacgtac
     8881 accgattgaa catgtgctgg acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg
     8941 acgcgggcag cgtatagggc tgtttgccgg gtcgggcgtt ggtaaaagtg tgctgctggg
     9001 gatgatggca cgctacaccc gcgccgatgt cattgtcgtg ggtttgattg gtgaacgtgg
     9061 gcgcgaagta aaagatttta ttgagaatat cctcggtgcc gaaggacgtg cacgctcagt
     9121 ggtgattgcc gctccggcgg atgtttctcc gctcctgcga atgcaaggtg ccgcctatgc
     9181 cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga ttatggactc
     9241 cctcacccgc tacgcgatgg cccagcgtga aattgcgctg gcgattggcg aaccacctgc
     9301 cactaaaggt tatccaccgt cggtgtttgc caaattaccg gcactggtcg agcgtgccgg
     9361 taatggcatt agcggcggcg gctcgattac cgcgttttat accgtgctca ccgaaggcga
     9421 tgaccagcag gaccccattg ctgactccgc gcgggcaatc ctcgacggtc acattgtgct
     9481 gtctcgccga ctggcggaag ccgggcacta tccggctatc gatattgaag cgtcgatcag
     9541 tcgcgcaatg acggcgttga tcagtgagca acattacgcg cgagtgcgca ccttcaaaca
     9601 gctgttgtcg agttttcagc gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg
     9661 cagcgatccg atgctcgata aagccatcgc cctgtggccg cagctggagg gctatttgca
     9721 acaaggcatt tttgaacgcg cggactggga agcgtctctc caggggctgg agcgtatttt
     9781 cccgacagtg tcataaccca ggagataacg gcagatggcg gaagaacagc tcaaaatgtt
     9841 gatcgattat cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac
     9901 cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca
     9961 gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg
    10021 agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc
    10081 actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc
    10141 catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc
    10201 acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa
    10261 gcattagcag gcgagacaac aactgacaaa gcggcccccc agttgctggt agcaacagat
    10321 aagcccacga caaaaggcga gccgctggtc agcgatattc ttgccgatgc gcaacaagcg
    10381 gatttactga tccctgtgga tgaaacactt cctgtcatca acgacgaaca atccacatca
    10441 acaccgttaa ccaccgctca gacgatgacg ttggctgcgg tggctgacaa aaatacgaca
    10501 aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg caagtctgag cgcccttttt
    10561 gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcaccgtc aaccgtgtta
    10621 ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac tacagcacag
    10681 cctgatgacg cccccggcac accagctcag ccattaacac cgctggtagc agaagcccag
    10741 agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg ctgacgccag cccgctaatc
    10801 actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg
    10861 ggttctcacg aatggcaaca atcattgagc cagcatattt cgctgttcac ccgccagggg
    10921 caacaaagtg cagagttgcg tctgcaccca caggatttag gtgaagtgca aatttccctc
    10981 aaagtggatg ataaccaggc gcagatccag atgatttcac cgcatcagca cgtacgcgcc
    11041 gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta
    11101 gggcaaagca acattagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa
    11161 cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg gggaagacga cgatacgctt
    11221 ccggttcccg tctctttaca agggcgtgta acaggtaaca gcggcgttga tattttcgcc
    11281 taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc
    11341 aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg
    11401 cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg
    11461 cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca
    11521 aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca
    11581 atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag
    11641 caacccgctc gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt
    11701 tttcacgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg
    11761 agattaaaac cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg
    11821 acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa
    11881 gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt
    11941 gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc
    12001 gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg
    12061 ctgttcaacc tgctgcgccg tagcccggac attaccgtcg gggccatccg cattcagccg
    12121 taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa
    12181 ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat
    12241 aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat
    12301 actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc
    12361 tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt
    12421 accaatatca ccacctcgcc gaacaacatt gtggttaaca cgccgttcca tgtggagatt
    12481 ggcaacctga ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg
    12541 gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat
    12601 aacctggtgc gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc
    12661 tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa
    12721 cccgatcgca tcatcgccca tgttgacggc gtcccggttc tgaccagtca gtacggtact
    12781 ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg
    12841 aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa
    12901 tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg
    12961 ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg
    13021 acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca
    13081 tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg
    13141 aaccgctgga tattctgatc aacggttatt taatcgccca gggcgaagtg gtggttgttg
    13201 ccgataaata tggcgtgcgg atcaccgata tcattacccc gtccgagcgg atgcgccgcc
    13261 tgagccgtta gtgatgaata accatgctac tgtgcaatct tccgcgccgg tttctgctga
    13321 gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc
    13381 ctggctggta aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat
    13441 tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc
    13501 acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc
    13561 agcaccaacg gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa
    13621 gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg caccggtcct tctctggctg
    13681 attacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct acctggcggt
    13741 ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt
    13801 ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt tggtttattg
    13861 cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg gctggcactg
    13921 tttttgacct tttttattat gtcgccagtg atcgacaaaa tttatgtcga tgcgtaccag
    13981 ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc gcagccgctg
    14041 cgtgagttta tgctgcgtca gacccgcgag gcagatttag ggttgtttgc cagactggcg
    14101 aataccggcc cgttgcaggg gcctgaagct gtgccgatgc gcattttgct cccggcctac
    14161 gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat cccttttttg
    14221 attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggttcccccc
    14281 gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg
    14341 ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg
    14401 gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg cactggcagc cccgctgttg
    14461 ctggtagcgc tggtcacggg ccttatcatc agtattttgc aggcagccac gcagattaac
    14521 gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat cattattgcc
    14581 ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg
    14641 tatatcatcg ggtagcccgt acaatgatgc aggagacaag cgatcaatgg ctttcctggt
    14701 taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc gcgccaattc
    14761 tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg atcacgttcg
    14821 ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc
    14881 tggccgtaca gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg
    14941 ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacat
    15001 ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc gcgcatcatg gatatgctgg
    15061 cattactgct gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata
    15121 cctttcacac cctgccgatt ggtagcgaac cgttgaacag caatgcgttt ctggcaccca
    15181 ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc
    15241 tgttgctgac cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca
    15301 tttttgttat tggatttcca ttaactctga ctgtcggcat ctctttaatg gcggcattaa
    15361 tgccgttaat tgcgcctttt tgcgaacatt tattcagtga aatttttaat ttgctggctg
    15421 atattattag tgaattacca ttaatataat tccgtaacgt ttatcatgct accctaagga
    15481 ttatccgaaa aataatatct gcgaacatct tccaggatac tcctgtagcg aaatatttgt
    15541 tttaagctca ctcacatatc gcaacattta ctttaagaca attccaggca aattatacaa
    15601 cactttacgg gatagtaagt ccgcctgaaa aattgcgaga gtggcgcatt aggtgaccca
    15661 tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga
    15721 gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt
    15781 aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt
    15841 tgatgacctt gccatagctt gtgattcaca gcgtccttca gtggtgttta ttaatgagga
    15901 ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag cacatcatta atcaacatcc
    15961 caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt
    16021 ggtcagaaaa aatttattga tcagttctaa atcgattaaa ccggaatctc tcgacgatat
    16081 ccttggcgat attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt
    16141 atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat
    16201 tcaaatctcc gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat
    16261 taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga
    16321 taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc
    16381 accagtcagg ctgaataaga ttactctgct ttctcaacaa agataccgtc ctgatgccct
    16441 gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgcaaccag gtacattgtg
    16501 ccttcactaa actcctcaac tgccagtacc actccaggac gacgcggacc gccatccgtt
    16561 ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta
    16621 gaacaatttc gtttttctgg cagcgccaag cgcgcgcgag tgctgatttt ctcgacggtc
    16681 tatacttaag agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc
    16741 gaggaaagat gatgaaaacc gcaaaagtgt acagcgatac cgcaaaacgt gaggtcagcg
    16801 tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca
    16861 gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat
    16921 tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa
    16981 tgcacaataa aaaaatcccg accctgaggg tgtcgggatg aaacttgctt aagcaagaag
    17041 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ctgcaatttt
    17101 tttcgctgac aagaaaatat tcgcgctatg aatgattaac gctacttatg aatagaaatg
    17161 tgacgcgcgt caccccttgg tggctctggt gtcaacggga tcgctcccga aaaaatcatc
    17221 tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcgaccatgt
    17281 tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc
    17341 atagttatga ctggcaaccg gcagccccct ggctcagccg tttacacgaa gccaatattc
    17401 ccgtcattct ctgtagcagt aaaactttag cggaaatgct gtacttgcaa aaaatgttgg
    17461 ggctacaagg cttaccgctg attgcagaga atggcgcagt gatccagctt gctgaacaat
    17521 ggcaggatat agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatctgcc
    17581 aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg
    17641 acgatgcaac cattgccgaa tggacgggat taagccgtag ctaggcggcg ctgacgcagc
    17701 tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta
    17761 tcgctcgtct gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtac
    17821 tggatgcctc tgccggaaaa gatcaggctg ccaactggat tatcgcgacc tatcaacaat
    17881 tgtcaggcag acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcctttac
    17941 tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtacatctgc
    18001 atgatgagga tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg
    18061 aagggctgga ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattgc
    18121 gcccggcctg ttttgcgagg taaagccgac ggtcggccaa tgactggagt tgttcaaaat
    18181 cataatcacc ggtttcctcg ctgctactta cccccagcga ggcactgatg cgtatcgttg
    18241 tactcttggc gatcagcatc tctttttcat ttaacttcag gcgaatacgt tctgcgacct
    18301 ccgcagcctg cgtcagattc gcgcctggca gaatcacaca aaactcctca ccaccgaccc
    18361 gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa
    18421 cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga
    18481 cctgaatgac agaaaaagga tgttggtgcg tctgacacaa tttagcgagc ggacgggctt
    18541 tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact
    18601 gcaacgagct ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga
    18661 ggagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga
    18721 aatcgccgcg cacgccttcg cttagcgtat ggacgcgcgc cagcacaccg tcgaaatggt
    18781 ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt
    18841 ccattgcctg cgccagcaag gccagttcac gaggatcaaa aatattccct gttggatggt
    18901 caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga
    18961 ggtttttatc gatggcgttt cttaaaaatt gctgcatagt acgcacggga atactcatcc
    19021 ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt
    19081 cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt
    19141 ctcgttgcga atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta
    19201 cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccagcacgc gagacataca
    19261 tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca
    19321 gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaaa ttcccctcgc
    19381 tgactaatgc atccgatacg ccattgactg gcagggtgcg ccgtcggttg agttcgattt
    19441 gccaggcgtg ctcgtcgcga tgctgttcga actcggttac ggcattacgc agagaggtga
    19501 aatccagtgg cgccacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga
    19561 cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctagc
    19621 tggagatata ggcatcttcc agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa
    19681 gcaataccac gataaagcag agattaacga catgaccggg ccccaggcgg cgtgcgagtt
    19741 ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc
    19801 tgctttttta tgattctggc ataacccggc aatagcgtac cgcaaaacag cgataatttt
    19861 ataatggtgg catgataaga cgcgactatc attcattgca ggcattggga caccaggccg
    19921 gatgtgatcc ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc
    19981 ctcccccgga acaagatcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata
    20041 ccaacgacac tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc
    20101 tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa
    20161 acttgttcac cttagttgaa gatggcgaat tttgcataat agtcagtgcg agtattaatg
    20221 tgcctgaccg cgcattttcg ctactgcttt cactcccagc accacgatgc cgccgatgat
    20281 aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg
    20341 cccggcgata tgttcaatag cgtgatgcag cggcgcaata ccatgtacca caatcccgcc
    20401 gccgacgagg aacatcgcca gcgtgccgac aatcgataat gctttcatca gccaaggcgc
    20461 gataatcaat aaccctttac ctaatgcctg catcagcgcg ctagattttt ccgccagcca
    20521 ataccctagg tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac
    20581 taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgcct ctgccacaat
    20641 ccccagcgtg atggcgacga tttccgcaga caaaataaaa tcggtacgaa tcgccccttt
    20701 aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg
    20761 gctctgcgct ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac
    20821 cccttcaaag cagagaaacg cgccaccaat catcaacagt ggcgtaatcg cccacgggat
    20881 aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt
    20941 cgccacgccc cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc
    21001 attgagcgat aaatcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat
    21061 cacagagata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc
    21121 ggccaaaagc attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg
    21181 cagctctgat acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct
    21241 ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc cgccgtgagg
    21301 gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat
    21361 ttggggctca acctattttg tcattcggat tggcgtggga agctggcctc cgttaatgat
    21421 ggcgggcgtt cgattccagg cagccggtat tttattgctg gcatttttgc tactgcgcgg
    21481 acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct
    21541 ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc
    21601 cgccgtagtg attgcaaccg tgcccctctt taccctgtgc ttcagtcgcc tgtttggcat
    21661 taaaacgcgc aaactggaat gggtgggtat tgccattggg cttgccggaa tcatcatgct
    21721 caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc
    21781 gattagctgg gcgtttggct cagtttatgg ctcgcgcatt accttacctg tagggatgat
    21841 ggcgggtgcg attgagatgc tggcggcagg cgtggtgtta atgatcgcat cgatgattgc
    21901 gggtgaaaaa ctgacggcgc tcccttccct gtcaggcttc cttgcggtcg gctatctggc
    21961 gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgta atgtcagtcc
    22021 ggctctcgcc accagctacg cctacgttaa cccggtggtc gcggtcttgc tgggtacggg
    22081 actgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc
    22141 ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt
    22201 tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac
    22261 cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgtc agcttctcgc
    22321 gcctacgtaa cgcacactcc cagacaatca gtacgcgcca gccgagctcc tgcaagcgac
    22381 tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcagttc
    22441 gagtcgcagg cactttaaac agatagcagt gatgatgatg ccagaagcag ccgtgggtaa
    22501 atatcacgca gcgatattca tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct
    22561 gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc gaggcgcttc tcaatcgccg
    22621 tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga
    22681 ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt
    22741 tttgccaccg cggcaaagac cggcacgacc accgagttgc cgaactggcg ataggcctga
    22801 gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc
    22861 gcgcattcac gaggcgttaa ccgacgtgga cgatgttgct gattcagcgg atcgtcaaag
    22921 tctttctcac ccgtggccat atcccaaccg cgatcgatta aaatttccgc gccatctttg
    22981 taataacgag cagacagcgt acgggtgacg ctttgcggat tgttcggata aaccattcca
    23041 taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac
    23101 agcaccggcg tcaggatata tttcgcctca accatcgggt ccagcagctg cgccagcgtc
    23161 actcgctgtg cagggaaaca ttcgctgata tcacgcagag taaaatcggc tttcagattc
    23221 agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag gaaatgttta
    23281 ccatcgatga ttttcggatc gtccggcccg ttatcttctg catcggcaac gtcatagccc
    23341 agttcgtcca gcgtctgcat gatgatgcgg aacgtttttc ccttatcgtg actcttcagg
    23401 tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca
    23461 tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc
    23521 tttttcgata cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg
    23581 tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga
    23641 tgactgaggg tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa
    23701 tggttggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt
    23761 ccaccaatcg attcaaaacc acgacggatg ccgccaattc cggcaaatag atcgataaag
    23821 cgaaacgcat aatgcggatg atgttccggt ggtttgggta acaacgtttg cagatgggca
    23881 aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt
    23941 gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcat tttaacatca
    24001 taaatttgca gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg
    24061 gttactgata tattttcctg catagattta accggccatc tgagatggcc gacaggttaa
    24121 cataattact gccgtttagg gccacgcgct cagccagcgg gtgaaaacgc atctatcacc
    24181 ttgtgatcga taccttcatt ctcccccgcc agttcggcac cgagcttcgc catcaaacag
    24241 cgccaagctc aatgcccctg aaacggcaaa tacgcgcgcc aggccaatcg cccccagggc
    24301 ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc
    24361 gctgaaactg tgagcggcaa tggcatgaca aacggcctca attttctccg ccggaaattg
    24421 cacgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg
    24481 ccgttgcgga tgatttttcg ccaggctcac gatatcgtga aaataacagg cggttaaaat
    24541 caccagcata tcgacatcgt catctgccgc cagtttctgt gccgtagccc agacacggcg
    24601 aaaatgacac aggtcatgcg ccgcgtcctg atgttgatga tggtttttca accagttctc
    24661 gaactatgcc tgccagtgtt gtaactccat acgcgctccg ttgccaaaga gtctcaacct
    24721 tagcagtttt tattcatagc gtctgcaacg tgcgggacga ggtcgggtgt accatgcaat
    24781 gccgcctaac aaggcaccaa ccagggccat aaacagaaat ccgaccaatg ctcctaacca
    24841 cttccccgcg gtagatccta aatcactttc aaatcccgac acgggggcat cggggatccg
    24901 gctcatcacc cagatgtatc gcgccattgc ccagacgaaa tagccacacc atgtatagaa
    24961 agccc
//
LOCUS       DAJTOW010000054        24730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTOW010000054 DAJTOW010000000
VERSION     DAJTOW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.054"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..1272)
                     /gene="yeaG"
                     /locus_tag="OO244_002326"
     CDS             complement(<1..1272)
                     /gene="yeaG"
                     /locus_tag="OO244_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5664121.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFA"
     gene            1708..2454
                     /gene="mipA"
                     /locus_tag="OO244_002327"
     CDS             1708..2454
                     /gene="mipA"
                     /locus_tag="OO244_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5664122.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2544..3305
                     /locus_tag="OO244_002328"
     CDS             2544..3305
                     /locus_tag="OO244_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5664123.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3356..4240)
                     /locus_tag="OO244_002329"
     CDS             complement(3356..4240)
                     /locus_tag="OO244_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5664124.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4324..5319)
                     /gene="gapA"
                     /locus_tag="OO244_002330"
     CDS             complement(4324..5319)
                     /gene="gapA"
                     /locus_tag="OO244_002330"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5664125.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5661..6074
                     /gene="msrB"
                     /locus_tag="OO244_002331"
     CDS             5661..6074
                     /gene="msrB"
                     /locus_tag="OO244_002331"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5664126.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6116..6388
                     /locus_tag="OO244_002332"
     CDS             6116..6388
                     /locus_tag="OO244_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5664127.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6758..7834
                     /locus_tag="OO244_002333"
     CDS             6758..7834
                     /locus_tag="OO244_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5664128.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFYSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7861..9240
                     /locus_tag="OO244_002334"
     CDS             7861..9240
                     /locus_tag="OO244_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664129.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9257..10302
                     /locus_tag="OO244_002335"
                     /pseudo
     CDS             9257..10302
                     /locus_tag="OO244_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10323..11159
                     /locus_tag="OO244_002336"
     CDS             10323..11159
                     /locus_tag="OO244_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5664130.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11164..12111
                     /locus_tag="OO244_002337"
     CDS             11164..12111
                     /locus_tag="OO244_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5664131.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12121..13101
                     /gene="ydjG"
                     /locus_tag="OO244_002338"
     CDS             12121..13101
                     /gene="ydjG"
                     /locus_tag="OO244_002338"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5664132.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13238..13996
                     /locus_tag="OO244_002339"
     CDS             13238..13996
                     /locus_tag="OO244_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5664133.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14114..15472
                     /locus_tag="OO244_002340"
     CDS             14114..15472
                     /locus_tag="OO244_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664134.1"
                     /translation="MEQYDQIGARLDRLPLVRFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15565..16206)
                     /gene="pncA"
                     /locus_tag="OO244_002341"
     CDS             complement(15565..16206)
                     /gene="pncA"
                     /locus_tag="OO244_002341"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5664135.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16217..17233)
                     /gene="ansA"
                     /locus_tag="OO244_002342"
     CDS             complement(16217..17233)
                     /gene="ansA"
                     /locus_tag="OO244_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5664136.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17400..19256)
                     /gene="sppA"
                     /locus_tag="OO244_002343"
     CDS             complement(17400..19256)
                     /gene="sppA"
                     /locus_tag="OO244_002343"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5664137.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19417..19968
                     /locus_tag="OO244_002344"
     CDS             19417..19968
                     /locus_tag="OO244_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5664138.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20085..21128
                     /gene="selD"
                     /locus_tag="OO244_002345"
     CDS             20085..21128
                     /gene="selD"
                     /locus_tag="OO244_002345"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5664139.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21133..23094
                     /gene="topB"
                     /locus_tag="OO244_002346"
     CDS             21133..23094
                     /gene="topB"
                     /locus_tag="OO244_002346"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5664140.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSVG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23221..24261
                     /locus_tag="OO244_002347"
     CDS             23221..24261
                     /locus_tag="OO244_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5664141.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24378..>24730)
                     /locus_tag="OO244_002348"
     CDS             complement(24378..>24730)
                     /locus_tag="OO244_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5664142.1"
                     /translation="AHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAG
                     GVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAG
                     FVKVADAMLAQGVI"
BASE COUNT         6292 a         5711 c         6388 g         6339 t
ORIGIN      
        1 cgcaaaacgc gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc
       61 ctgatacgac ttggctttcg gatcggtgtc tttcagactt tcgccatcat aaacccgcat
      121 ctttgaataa atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga
      181 cagtgtttcg agcgtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa
      241 tttctcgtag attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta
      301 aacacggtcg aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactt
      361 gttcgagtgg gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt
      421 gtagttacct tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa
      481 catctcaacg aattccatga tcccctgatt ggcgcggcac agcgcaccgg aatagccgta
      541 ggcgtccggg tcattctgcg cgtagtgttc gagtttacga atatcgactt tcccaaccag
      601 cgcggagatg tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg
      661 cagaattgac ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg
      721 caggcgtttt gccgcccatg gcgacatgat ggtgccgaga taacggcgag gaatgccata
      781 ctctttttcc agaatctgag catcttcctg cggattgaaa agacagaacg gatgatcgtt
      841 gaccgggcta cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga
      901 tttcagtcgc tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat
      961 ttgtttcttc tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat
     1021 cgcgtcttcc atgccgtaaa actcttcaaa tgccggataa cgtgcaatga cccggttaga
     1081 aaagagtcga gaaagtctgg ggtcctgggc tgtatcgacc atgacaggct caccgatagc
     1141 catcaatagc cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag
     1201 aaactcctgc agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc
     1261 gaatatattc atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat
     1321 gaatagtaga ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa
     1381 ctctcatgcc attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta
     1441 agcgtagatg gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca
     1501 acaactcatc cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa
     1561 atccatttgg ctgtaagcgc ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa
     1621 aaatgaaaac gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct
     1681 aaacatttgt ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg
     1741 cttatcgcaa cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc
     1801 gtaggtgtcg ttgagcaccc atataaagat tacgataccg atgtttaccc agtaccggta
     1861 atcaactatg aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg
     1921 aatgacgcaa cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagcg
     1981 aaagacagtg gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct
     2041 ggtctgtctt atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat
     2101 accctggata acagcaacgg cattgtctgg gatatggcct ggttgtatcg ttacaccaac
     2161 ggtggcctga ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa
     2221 tactattatg gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg ctataacccg
     2281 aatgacagct ggagccctta ccttgagctg agcgccagct acaacttcct cggcgactgg
     2341 agtgtttacg gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatg
     2401 gtggataaat cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc
     2461 atctttccag ggcattttgt gcataaaaac agggcgtcac gccctgtttt gcattacggt
     2521 ggtgcaatac ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct
     2581 cactgccagc catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa
     2641 cagaagttgc tgcactacgc gcaggcattg aactcggttt aaccctcatt gataccgccg
     2701 aaatgtatgc cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag
     2761 agaaggtctt tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa
     2821 atgcatgcga agccagttta cgccgtctca atactgacta tctcgatctc tacttattac
     2881 actggtctgg cagtttcgct tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc
     2941 agggaaaaat ccgtcgctgg ggcgtttcta accttggtta tgccgatatg caggaactct
     3001 ggcagctgcc ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac
     3061 gagggattga gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt
     3121 acagtccatt agcccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca
     3181 acgaaattgc acatgtccaa caacatgcag ctgcgcttga ggtcgaactt tcgtcagcgg
     3241 aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg ctggatatgg
     3301 tgtgatgttg atacgtgccg gatgcatcgc acccggcaca caacattaac gcaaattaac
     3361 gtttggcaac gcgaatggat tgcgccagat gtgcaggttt ctctttggtg actttttgcg
     3421 tttctgaagc gtaagccgtt tctacacaaa caaaggtctt gtagccatcg tccggcatgt
     3481 cgcccatgct aactgaaagc gccggtcccg ggttccagcc gacaacgttc agatgatgct
     3541 ggtggcctac ggcgataata cgattcagcg cttcatcatt aatcacgctg caatcttgcg
     3601 gattcagata cacgcgatcg gtacggtcag ggaaggtctg aataccgtcg gtcagtacat
     3661 cttctttcgc gtcattcact ttatcaatga agcgatcgcc cagcccactg acgcttacct
     3721 tagcgatatc acccacgtta aagtaggtat gcagggcaga ggtggtttca aattcgccat
     3781 gtgattcaag atcgatttca caggttttac ccacgcggaa atgcgctaac agcgtaaagt
     3841 cgtgcggcca gaattttttc gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc
     3901 catcagcatc ttcatgatgt gatttcagcg tccacggcag gttgcgggca aaaccgtgcg
     3961 caggcagacc ttgttgtgcc gccggaccaa accacggcca gcaaaccggt acgccaccgc
     4021 gaatagcgac gccatttttg aacggtgtgt tgttgctcaa ccacagaact tcttcttcac
     4081 ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg
     4141 gatgatcgac cacaatgagg tccagttcat ccagtttacg acgggagagg acaggggaga
     4201 tttgttcgat gaccggaagg gcaaaaattt tcttaatcat gacgcagtcc tttaacttca
     4261 ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc
     4321 aacttatttg gagatgtgag cgatcaggtc cagaactttg ttggagtaac cggtttcgtt
     4381 gtcgtaccag gataccagtt tcacgaagtt gtcgttcaga gcgataccag ctttagcatc
     4441 gaacacggaa gtgcaaactt cgccgttgaa atcggtagat actacgtcat cttcggtgta
     4501 gcccagaacg cctttcattt cgccttcagc agcagcttta acggcagctt tgatctgctc
     4561 gtaagttgca gctttttcca gacgaacggt caggtcaact acggatacgt tcggggtcgg
     4621 aacgcggaac gccataccag tcagtttgcc attcagttct ggcagtactt tacctacagc
     4681 tttagcagca ccggtagagg acgggatgat gttctgggaa gcgccgcggc cgccgcgcca
     4741 gtctttgtga gacgggccat caacggtttt ctgagtagcg gtagtagcgt gaacggtggt
     4801 catcagacct tcgatgatgc cgaagttatc gttgataact ttagccagcg gagccaggca
     4861 gttggtggtg caggaagcgt tggaaacgat gtcctggcca gcatatttgt cgaagttagc
     4921 gcctttaacg aacatcggag tgttgtcttt agacggacca gtcataacca ctttcttcgc
     4981 accagcggtg atgtgtttac gagcagtttc gtcagtcagg aacagaccag ttgcttcagc
     5041 gacaacgtca acacccactt cgtcccattt caggttagcc ggatcacgtt cagcggtaac
     5101 acggattttt ttaccgttaa cgatcagatg accgtctttc acttcaacgg taccgtcgaa
     5161 acggccgtga gtggagtcat atttcagcat gtatgccatg taatcagcgt ctaacaggtc
     5221 gttgattgca acgatctcga tgtcagaacg tttctgagca gcacggaaaa caatgcgacc
     5281 gatacggcca aaaccgttga tacctacttt gatagtcata tattccacca gctatttgtt
     5341 agtgaataaa aggttgcctg taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt
     5401 gtcaatcatt gcgacaaatc aatcctgtgc ctaagcatta cgcgactgac tcgcctcact
     5461 tttcctttgg gcttgagacc acatggggtc ggcgccccga attttaaagg gcaattacga
     5521 taaaaatgtg attttcatca cgatttaggt gcagccattt cgtcgagatc aagtttagga
     5581 caaaattttg ccagttggtg ttaatgtttt gttagaatca gtcacgtaat gtgagcacga
     5641 ttaaagtgag atgtgagtaa atggcgaata aaccttcggc agaagaactg aaaaaaaatt
     5701 tgtccgagat gcagttttac gtgacgcaga atcatgggac agaaccgcca tttacgggtc
     5761 gtttactgca taacaagcgt gacggcgtat atcactgttt gatctgcgat gccccgctgt
     5821 ttcattccga aaccaagtat gattccggct gtggctggcc cagtttctac gaaccggtga
     5881 gtgaagaatc cattcgttat atcaaagact tgtcacatgg aatgcagcgc atagaaattc
     5941 gttgcggtaa ctgtgatgcc catctggggc atgtctttcc cgacgggccg caaccaacag
     6001 gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac tgatggcgaa aacggcgaag
     6061 aaatcaacgg ttgaaaaaac gattcagcaa cttattccac aggagcggaa aaattatgaa
     6121 tcttgatgac attatcaaca gcatgacgcc tgaggtatac cagcgtttgt cgaccgccgt
     6181 tgaactgggg aaatggcctg atggcgttgc gttgaccgag gaacaaaaag agaattgcct
     6241 gcaactggtg atgctgtggc aagcccgcca taataccgaa gcacagcata tgacaattga
     6301 cacaaatggt cagatggtca tgaagagcaa acagcagtta aaagaagatt ttggtatctc
     6361 agcaaagcct attgccatgt ttaagtaagt ggttcatccc ctttttgccg cggctatgaa
     6421 ggggatagcc aacaatgacc ccacgactcg tctcaattat ctcgacccga cctggctaag
     6481 tcccagtccg atctcttgca gtcgcgttca cgcctgaatg acttctcctg aacttaagtc
     6541 tcagacctat ttggccggta atccctctcg aatttgctct ttttgcgtcc cgccttccac
     6601 caacctgtaa cagacccagc cattttctgc gtaattgtga tttgagttgt aaaatgcagg
     6661 tcattatctg tgttgtgcag atttaatatt gacctcaaaa atcatgtggg taagtatgat
     6721 ggccatctaa gcaaaacaaa acacagagga tgacgcaatg aaagcactgg ctcggtttgg
     6781 caaggccttt ggcggctaca agatgattca tgtcccacaa cccatttgtg gcccggaaga
     6841 cgtagtgact gaaattaaag ccgcggcaat ctgcggcgca gacatgaagc actacaatgt
     6901 cgatagcggt tctgatgagt tttactctat ccgcggccat gagttcgcag gttgtattgc
     6961 gcaggttggt gaaaaagtca aagactggaa agtggggcaa cgcgtcgtgt cggataacag
     7021 cggtcacgtt tgcggcgttt gtccggcctg tgaacagggt gattttctgt gttgtacaga
     7081 aaaagtaaac cttggtctgg ataacaatac ctggggcggt ggtttttcca aatattgtct
     7141 ggttcctggt gaaattctca aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt
     7201 tgattatgag gacgcagccg tacttgaccc tatctgtaat gcctataaat ccatcgcgca
     7261 gcaatcgaaa ttccttcctg gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct
     7321 gttttccgta caaatggcgc ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca
     7381 agaagatgtg gcggtccgct tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa
     7441 tggttctacc gaagatgtgg tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg
     7501 actggtgatt gaatgctccg gtgccaatat cgcactgaaa caagccatcg aaatgctccg
     7561 cccgaatggg gaagtggtac gcgttggaat gggcttcaaa cctcttgatt tctcgattaa
     7621 tgacattacc gcctggaata aaagcatcat tgggcatatg gcctatgact ccacctcatg
     7681 gcgtaacgct atccggctat tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca
     7741 tcgtatcggc ctgtcgcaat ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat
     7801 caaagtgatc atgacttacg actttgatga ataactactc tcctgaagga acgttatcgc
     7861 atggaacaga taacaaaacc gcattgtggt gcacggctgg atcgcttacc ggattgccgc
     7921 tggcattcgt caatgtttgc catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc
     7981 gttggtggct tgatcctcgc gcagctgaaa gcgttgggct ggacagataa ttccactaca
     8041 gccacattct cagcaatcac gaccgccgga atgtttctcg gtgcgctggt gggcggcatc
     8101 attggcgaca aaaccggtcg cagaaatgcg ttcatcctct atgaggccat tcatattgcc
     8161 tcgatggttg tcggtgcttt ctcaccgaat atggattttc ttattgcctg ccgttttgtg
     8221 atggacgttg ggctgggagc tttactggtt acactgtttg ctggtttcac cgaatatatg
     8281 cccggtagaa atcgcggaac gtggtccagt cgggtttcct ttattggcaa ctggtcatat
     8341 ccgctctgct cactgattgc gatgggactc acgccgctga ttagtgcaga gtggaactgg
     8401 cgggtacaac tgcttattcc tgcaatattg tcgcttatcg ctacggcgct ggcttggcgc
     8461 tactttcctg aatccccgcg ctggctggaa tcgcgcggac ggtatcagga agccgagaaa
     8521 gtcatgcgga gtatagaaga aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg
     8581 gttattgctg atgacggtaa agcgccacaa gcggtgccgt attcagcctt actgacagga
     8641 gtattactga aacgcgtgat attaggctct tgtgtgctga ttaccatgaa cgttgtgcag
     8701 tacacactaa ttaactggtt gccaacaata ttcatgaccc aggggattaa tttaaaagac
     8761 tcgattgttt taaataccat gagtatgttt ggcgcgccat ttggtatttt tattgccatg
     8821 ctggtgatgg ataaaattcc gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg
     8881 gtgctcggat atatctattc actgcaaacc agtatgttgc tcataacgct gattggtttc
     8941 ttcctgatta ctttcgtcta tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa
     9001 atctggccga cagaggccaa actccgtggc tccggtctgg cgaatgcggt agggcgaatc
     9061 agtggtattg ccgcacctta tgccgttgca gtgctgctca gtagttatgg tgtaacggga
     9121 gtctttattc ttctgggggc tgtttcaaat attgtcgcaa ttgctatcgc caccattgga
     9181 attgaaacca aaggtgtctc cgttgaaagt ttaagtattg atgcagtagc caataaataa
     9241 tacgggtgga ttcaaaatga aaaattcaaa agcaatattg caggtgccgg gcacaatgaa
     9301 aattatttca gcagaaatac cagtgcctaa agaagatgaa gttttgatta aagtagaata
     9361 tgtcggtatt tgtggttcag atgtacatgg ttttgaatca ggcccgttta ttccgcctaa
     9421 agacccaaat caagaaattg gcctgggtca tgaatgcgcc gggacggttg tggctgtggg
     9481 aagccgcgtg cgcaaattta aaccggggga tcgggtaaat atcgaacctg gcgttccttg
     9541 cggtcactgt cgttactgtc tggaaggcaa atataacatc tgcccggacg ttgattttat
     9601 ggcgacacaa cccaactacc gcggcgcatt aacgcactat ctgtgtcatc cggagagctt
     9661 tacttacaaa ctgcccgaca atatggacac gatggaaggg gcgctggtgg agcctgccgc
     9721 agtcgggatg catgccgcga tgctggcaga tgttaaaccg ggtaagaaga taattattct
     9781 gggagcaggt tgtattggtt tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga
     9841 aattgccgtc gttgatgtac tggaaaaacg tctggcaata ggggaacagc ctggtgcgac
     9901 agtggttatt aatggcgcaa aagaggacac tattgcacgc tgtcagcaat ttaccgaaga
     9961 catgggcgca gatattgttt tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc
    10021 tttaatctgg taatgcgcgg cggtaaaatt atgattgttg gtactgtacc cggcgattcg
    10081 gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc agacggtatt ccgctatgcc
    10141 aatcgttatc cggtcacgat tgaagctatt tcttcagggc gattcgatgt gaaatcgatg
    10201 gtgacgcata tttacgatta tcgggatgta caacaggcat ttgaagagtc agttaacaac
    10261 aaacgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    10321 acatgctcgc agatatcaga tattgggaaa acgatgctac aaataaatat tatgcaattg
    10381 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    10441 cgaaatcccc ggttattatt tcttttagta caggttttgt cggtaacacc tcatttgaag
    10501 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    10561 gggatcatgg tcggagtatt gagattattc ataacgcctg gactcatggt atgaattcat
    10621 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    10681 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    10741 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    10801 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    10861 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    10921 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    10981 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtag
    11041 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    11101 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    11161 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    11221 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    11281 accgggggcg atgcaataaa tgaagcaaca attatttctc gtctggggca tcgtaccgca
    11341 ttaatgagcc gcattggtaa agatgcctca gggcaattta ttctcgacca ttgtcgcaaa
    11401 gagaatattg atattcaaag cctgaaacag gacgtcagca tagatacatc tattaacgtt
    11461 ggactggtga cggaggatgg cgagcggacg tttgtcacga accgtaacgg cagtttgtgg
    11521 aaattaaata ttgacgatgt tgattttgcg cggtttcctc aggcgaaatt attatcactg
    11581 gccagtattt tcaacagtcc tctattggat ggtaaagcgc taacagaaat ttttacgcaa
    11641 gcgaaagccc ggcagatgat catttgtgcc gatatgatca aaccgcggtt gaatgaaacg
    11701 ctggatgata tttgcgaagc attgagttat gtcgattatc tgtttccaaa ttttgccgag
    11761 gaaaaattac tcacagggaa agagacactg gatgaaattg ctgactgctt tcttgcgtgc
    11821 ggcgtaaaaa cggtggtgat taaaacgggt aaagacggct gctttatcaa gcgtggtgac
    11881 atgacgatga aggtgccggc ggtcgcagga ataaccgcca tcgacaccat tggcgcgggc
    11941 gataactttg cttcaggttt tattgcggca ctgttagaag gcaaaaatct gcgtgaatgc
    12001 gcacgctttg ccaatgcaac ggcggctatc tcggttctaa gcgtcggtgc caccaccggc
    12061 gtaaaaaaca gaaagctggt cgaacaattg ctggaagaat acgaaggata atgaaggcaa
    12121 atgaaaaaga tacctttagg cacgacggat ataacgcttt cgcgaatggg gttggggaca
    12181 tgggccattg gcggcggtcc tgcatggaat ggcgatctcg atcggcaaat atgtattgat
    12241 acgattcttg aagcccatcg ttgtggcatt aatctgattg atactgcgct aggatataac
    12301 tttggcaata gtgaagttat cgtcggtcag gcgttaaaaa aactgccccg tgaacaggtt
    12361 gtagtagaaa ccaaatgcgg cattgtctgg gaacgaaaag gaagtttatt caacaaagtt
    12421 ggcgatcggc agttgtataa aaacctttcc ccggaatcta tccgcgaaga ggtagaagcc
    12481 agcttgcaac gtctgggtat tgattacatc gatatctata tgacgcactg gcagtcggtg
    12541 ccgccatttt ttacgccgat agctgaaact gtcgcagtgc ttaatgagtt aaaggctgaa
    12601 gggaaaattc gcgctatagg cgctgctaac gtcgatgctg accatatccg cgagtatctg
    12661 caatatggtg aactggatat tattcaggcg aaatacagta ttctcgaccg ggcaatggaa
    12721 aacgaactgc tgccgctatg tcgtgataat ggcattgtgg ttcaggttta ttccccgcta
    12781 gagcagggat tgttgaccgg caccatcact cgtgattacg ttccgggcgg cgctcgggca
    12841 aataaagtct ggttccagcg tgaaaacatg ctgaaagtga ttgatatgct tgaacagtgg
    12901 cagccacttt gtgctcgtta tcagtgcaca attcccactc tggcactggc gtggatatta
    12961 aaacagagtg atttaatctc cattcttagt ggggctactg caccggaaca ggtacgcgaa
    13021 aatgtcgcgg cactgaatat caacttatcg gatgcagacg caacattgat gagggaaatg
    13081 gcagaggccc tggagcgtta aatactcaac acgattcacc tctgtttctg tgaaaactgt
    13141 gatgaaaatc tgtgattcaa ataggttatt ttgaaagtaa acatcggtta tgcgataatc
    13201 gcgctaatgt gatgtaaaaa gatacagggg tgtaatcgtg gcggcaaaag acaggattca
    13261 ggcaattaag caaatggttg ccaacgataa aaaggtgaca gtctcaaatt tgagtgggat
    13321 ttttcaggta accgaagaaa ccattcgccg cgatcttgag aagctggaag atgaaggctt
    13381 tttgaccaga acctatggtg gtgctgtttt aaatacagcg gtgttgacgg agaatatcca
    13441 tttttataag cgcgcttcat cgttttatga agagaagctg cttattgcac gcaaggcact
    13501 accctttatc gacaataaaa ccaccatggc agccgattcc agtagtaccg taatggaatt
    13561 gctcaaattg ttacaggacc gtagtgacct gacgttgcta accaactcgg cggaagcgat
    13621 tcatgttctg gctcagtcag aaattaaagt cgtttcaacg ggtggggaac taaacaaaaa
    13681 tacactttcc ctgcaaggaa gaattactaa agagatcatc agccgctacc atgtcgatat
    13741 catggtaatg agttgtaaag gtcttgatat taacagcggc gcgctggact ctaatgaagc
    13801 ggaagctgaa atcaaaaaga caatgatccg tcaggcgaca gaagttgcgt tattggttga
    13861 tcactctaag tttgatcgta aagcttttgt ccagttagct gactttagtc atattaatta
    13921 tataataact gataaatcac cgggtgcaga gtggatagca ttttgcaaag acaataatat
    13981 ccagctggtg tggtaattta agcacacgtt gtcaatatta tttgttcctg cgactggaaa
    14041 taaaatgatg ataaagatgc ggcaagtgaa tttgccgcaa ttgctacctt ttatagcaaa
    14101 tccgaggcac ccaatggaac aatatgatca aattggcgca agactggacc gcttgccttt
    14161 ggtccggttt cattatcgta tatttggtat aataagcttt agtctgttat taacggggtt
    14221 tttgagttac tcaggtaatg tcgtcttagc aaagctggta agcaatggat ggtcaaataa
    14281 tttcctctat gccgccttta cctcggcatt aatgtttggt tatttcatcg gctcacttac
    14341 tggtgggttt attggtgact actttgggcg gcgcagggcg tttcgcataa atcttctcat
    14401 cgtcggtatt gctgcaacag gggccgcttt tgtccctgat atgtactggc tcattttctt
    14461 tcgcttcctg atgggaacag gaatgggggc gctgattatg gttggctatg cctcatttac
    14521 ggagtttatc cccgcgacgg tgcgtggaaa atggtccgcg cggctctcat ttgttggtaa
    14581 ctggtcgccc atgctgtctg cggcgatagg cgtggtggtt atcgcttttt ttagttggcg
    14641 aataatgttt cttctgggtg gtattggcat actgttagcc tggtttctct caggtaaata
    14701 ctttatcgag tcgccacgat ggctggaagg gaaagggcaa atcgccggtg cagaaagcca
    14761 acttcgtgaa gtagagcagc aaattgaaag ggagaagagt attcgtttac cccagcttac
    14821 tttgaaccag agcaacagca aggttaaggt aatcaagggt accttcaggc tcctgtttaa
    14881 aggggaaatg ttacgacgta cattagttgc gataactgtt ttaattgcaa tgaacatttc
    14941 gctttatacc attaccgtat ggataccgac catatttgtt aactccggca ttgatgtcga
    15001 taaatcaata ttaatgaccg ctgttattat gattggtgct ccggtaggaa tatttattgc
    15061 ggcattaatt attgatcatt ttcctcgtcg attatttggc tccgccttac ttattattat
    15121 tgccgtgtta ggctatatct attcaagtca gactacagag tgggcgattt taatctatgg
    15181 tctggtgatg atcttctttt tatacatgta tgtttgcttc gcgtcggcgg tttatatccc
    15241 ggagctttgg ccaacgcatt tacgcctgcg cggttcgggt ttcgttaatg ccgtcggacg
    15301 gatcgtcgca gttttcacgc cctatggcgt tgcggcatta ttaacacatt atgggtcgat
    15361 cacggtgttt atggtgcttg gtgtcatgtt agtgctctgt gcgctggttc tctccatttt
    15421 tggcatcgaa acgcggaagg tgtcgctgga agagatttct gaggtgaatt aaatctttcg
    15481 ttgatggcat caattgccag atgtaccact gacgtgtttt atttggccta caatgccagt
    15541 acagaattgt aggccagcgt aaaattaccc ctgcgtctct tcccagtctg ccagcgtata
    15601 tagcgttgcc ccagctgctg acatctccat aaacgcgtgt gcactgtcct ggggctggat
    15661 attcacgcca cgacagccat cggtaatcac gtttacctta taacctaact gtaacgcgtc
    15721 cagcacggta aacttcacgc aatagtcagt agccaggccc ataacgatca attcatcgat
    15781 ttcatgatcg cgcaaccagt catcaagcgc ggttttctgc cgacgtccgt tatcaaaaaa
    15841 ggcactgtaa ctgtcaacta aaggattttc gcctttatgg aacaccgctg cgatctcttt
    15901 ttgattcagt aacggatgta attgtgcgcc ttcactgttc tgcacacagt gttcaggcca
    15961 gaaagtttgc cgcaggccgc cgagttggcc tggcgtataa ggctctacgc cgtgctggct
    16021 ggcaaagctg ccgtgattcg ccggatgcca gtcctggctg gcgataaccg cttctccgcg
    16081 cgactggcac cagtcaataa ggcggttagc aacctccacc gtactgtcac cttccggtac
    16141 ggcgagcgcg ccaccagcac agaaatcatt ttgtaaatcg accagtaaca gggcgcgatg
    16201 gggcattgca gtctccttaa tcatccggcg tcagttcgcc gcgcaggttt tggctcatgg
    16261 ccttgcgaat ggtttcagta tccagttcct ggctcagcag gtaatgcagt ttggttagcg
    16321 tggcttctac agtcatatct gcaccgtcaa taacgccggc atgggcgagg gcgttaccgg
    16381 tggcataacc actcatattc actttaccgg acatacattg tgtcaggttg accaccacaa
    16441 taccgcgatc gctggcttct tgtaattcct gcaggaaggc tttgttttgt ggcgcattac
    16501 ccacgccata ggagcgcaga atcaatgctt tcaccggttg gcgcagaaaa ttgcgcacga
    16561 cgtcagcaga aatccctgga taaatcgtca ccacgccaat tggttgtggc gtgattggat
    16621 gaacgattaa ttccccttca ccgtgcgggg cgggtggcgt attcaaacga cgaatatgga
    16681 taccagcttc cagtaacgga ggaaggtttg gagaggcaaa cgcatcaaaa ccatcggcat
    16741 gggctttggt agtgcggttg ccgcgataca atcggttatt gaaaaagagc gttacttcgt
    16801 tgatcggata attcgccgca acgtacaacg cattcagcag attaatttgt ccgtcagagc
    16861 gtaactcagc cagcgggatt tgtgaccctg tcacaatgac cggtttaccg agattctcga
    16921 gcatgaacga cagcgcagag gcggtatacg ccatcgtgtc ggtaccgtgt agaatgacaa
    16981 aaccatcata gtcgtcatag tgcgctttaa tatcttcagc aatatgctgc cagtcttccg
    17041 gcgtcatatc agaagaatcc atcaacggcg tatattcatg aatggtgaaa tctggcatct
    17101 ccgggcgatg gaattccggc atcagcgcca gttggcgttg tagatgacct gacaccggaa
    17161 tataaccctg ctcggaacgc tgcatcccga tggtcccgcc cgtgtaggcg acgtaaattg
    17221 atttcttttg catgatattg atatgaatga tatcgaaaga gcaaaagtat atacgtgagg
    17281 caggaggagc gcacctgata ctatgcggat aaggaaatat ggggcgagac agcgggaaga
    17341 ggattcgtcg aggtaaaaaa taatgtggcc gacgatctgt cggccactca gtacaagact
    17401 taacgcacgt tggcgcaggt caggcaaaac gcataacggt tttgtgggtc gttaaacgcg
    17461 gccagcttgt cgctttcact tttaacggta gaggctaccg aggcaagcgg tgcaggtaac
    17521 atggcctgga acgcatctgg caacattgcc cggacagaac cagacatgtt gtccatcact
    17581 ttgtcgaaga aggtcggttc atcaacgtag tattccagat gccactgttt cacttttgcg
    17641 agctctgctg ctttggcaac cgcatcatcg aaatccccga gactatcgac cagcccgtta
    17701 gcttttgcat cctgaccggt ccagacgtgg ccctgggcga ttttatcaat ttgctccggc
    17761 gtcgaatgac gcgcatcagc aaccagcgtg ataaagcgtt tatagccatt ctcaatgctt
    17821 aattgcatca tctgctgcgc ttccggcggc agtgccctgg tgatagaaac atccgccagc
    17881 ggtgaagttg agacaccatc agtatgaaca ccaatcgaat ccagactatt ttctacggtg
    17941 gtgatcacgc cgaagatacc gatagaaccg gtcagggtgc tggggttagc cacaatgtaa
    18001 ttagctggcg tggaaatcca gtaaccacca gatgccgcca tgccgcccat cgatacaacc
    18061 acagacttac ccgctgcccg ggctgctgcc agttcagcgc gaatcacttc agacgcggta
    18121 acgctgccgc ctgggctatt aacacgcagg acaatcgctt tcactttcgg gtcaaggcga
    18181 gcgtcgcgga tttgtgccgc agtggtatca ccgccaacat tcccctgagt ttcctcgcca
    18241 tccataattg cgccattagc gaagacgaca ccgatgctgt caccggtatc tgccggcgtt
    18301 ttcaatgcgt aatcgtaata actgatggcg cgataatttt tatcagtctt actccagccg
    18361 aactctttgg tcagagcttt ttcgatttcg gcactcgatg ccagtgcatc gaccagcttg
    18421 ttttccagtg catatttcgc ggtatcgcca ccggttttgg ttaaaccctc aagcaaccct
    18481 tgcgcgccag ggaatacctg ctgagcaggg atctgccggt tagcggcaac agtattcaga
    18541 tagttttgcc acagctcacc aatccagcgg ctgtcagctt cgcgggctgc cggtgacata
    18601 tcatcacgaa taaacggttc aacggcagat ttatacgtac ccacgcggaa cacatgggtg
    18661 gaaactttca gcttatccag caacgatttg tagtacagac cgttggtagc aaaaccgtgc
    18721 agatcaacca cgccttgcgg tgacagccaa attttattgg cgaaactggc gagataatat
    18781 tgcccctggc tgtagttctc gccaacggca taaaccggtt tcccgctgtc acgaaactct
    18841 ttcagggctt tgccgatgta ctgcatagac ggctggtcgc cgcctgcgaa gtttttcaga
    18901 tccatcacga taccggtgat attgcggtcg tccttcgcct ggcgaatggt gttgacgata
    18961 tcaaacagtg agttttcctg cagacgatcg gaactggcac caagcagctg gcggcttaat
    19021 ttactaaacc gctgagaact gtctggttta tcgacgatca caccagaaat gtccagcagc
    19081 agtgcgccac gactggccgt ttctttcgaa tcaccaccac tgacctgcat ccagatcccc
    19141 acaccaacca gtacgaggaa aataaagaac aggttaagta ccatttcacg gacgaaattc
    19201 agcagacgcc acgtccattt aaaaaatccg gcaataaatc gccaaagggt tcgcatgtat
    19261 tctcccaact taaggtcaca cctgtcacag gcgcaataga tgtggttatc gtaatgaccc
    19321 aaccggcact tgtcagcagg aattgcggac tgtgctgtaa caaaatccac tctcgtgtta
    19381 attttgtgaa taaatatcac gacaggagtt aatcaaatgg atgcactcga actattgatc
    19441 aatcgccgta gcgcctcccg cctggctgaa cccgcgccaa cgggtgaaca actgcaaaac
    19501 atcctgcgtg cgggtatgcg tgcgccggac cataagtcca tgcaaccgtg gcattttttt
    19561 gtgattgaag gggaagggtg cgagcgtttc agcgccgtac tggaacaggg ggcgattgct
    19621 gccggtagtg atgataaagc tatcgacaaa gcccgtaatg cgccgttccg cgcaccgctc
    19681 atcatcacgg tggtggcgaa atgcaaagaa aatcataaag tcccgcgatg ggaacaggaa
    19741 atgtctgccg gatgcgcggt catggcgatg caaatggcag ccgttgccca gggatttggc
    19801 ggcatctggc gcagtggcgc attaactgaa agtccggtag tgcgtgaagc attcggttgc
    19861 cgtgagcagg ataaaattgt cggttttctc tacctcggca cgccgcagtt gaaagcatct
    19921 acgtcgatta acgtcccgga cccgacgccg tttgtaactt atttctgata attgtcgcga
    19981 aactgtctgg attgtgagca aaggcgcagg aattcagaca ctctcactta tcacttcacg
    20041 gaatgagggt taccataccc ggattgcatt gacaggagat gtccatgagc gagaactcga
    20101 ttcgtttgac ccaatacagc cacggagctg gttgcggctg taaaatttcc ccaaaagtgt
    20161 tggaaaccat cctgcacagt gagcaggcga agtttgttga tccgaatttg cttgtgggta
    20221 atgaaacccg cgacgatgcg gcggtgtacg atctgggcaa tggcaccagc gttatcagta
    20281 ccaccgactt ctttatgccg atcgttgata atcctttcga ttttggccgc attgcggcga
    20341 ctaacgccat cagcgatatc ttcgcgatgg ggggcaaacc gattatggcg attgcgatcc
    20401 tcggctggcc gattaacaaa ctttccccgg aaattgcccg cgaagtaacc gaaggtggac
    20461 gctatgcatg ccgtcaggcg ggtattgcgc tggctggcgg tcactccatc gatgcgccgg
    20521 agccgatttt tggtctggcg gtaacgggga tcgtaccgac cgagcgggtg aagaaaaaca
    20581 gtaccgcaca agccggatgc aaactgttcc tgacgaaacc gctggggatc ggcgttctta
    20641 ccacggctga gaaaaaatca ctgttgaaac cagaacatca gggactggcg acggaagtga
    20701 tgtgccggat gaacatcgca ggcgcgtcct ttgccaacat cgaaggcgta aaagcgatga
    20761 ccgacgttac gggctttggt ctgctgggcc acttgagcga aatgtgtcag ggggctggtg
    20821 tgcaggcacg cgtcgactat gaagcgatcc cgaaactccc cggtgttgaa gagtacatta
    20881 agttgggcgc agtacctggc ggcactgaac gtaactttgc cagctacggt catctgatgg
    20941 gtgaaatgcc gcgtgaagtg cgcgatctgc tgtgcgatcc gcaaacttct ggcggtttgc
    21001 tgctggcggt catgccggaa gcagaaaatg aggttaaaac tacagccgcc gagtttggca
    21061 ttgaactgac ggcgattggc gaactggtgc cagcgcgcgg cggtcgtgcc atggttgaga
    21121 tccgttaatt taatgcggtt gtttattgcc gaaaaaccga gtctggcgcg cgccattgct
    21181 gatgtcctgc ccaaaccgca ccggaaaggc gatggcttta tcgagtgcgg taatggtcag
    21241 gtggtgactt ggtgtatcgg tcacctgctt gagcaggcgc agccagacgc ctacgacagc
    21301 cgctatgcgc gctggaatct tgcggatttg ccgattgtcc cggaaaagtg gcaattacag
    21361 ccacgaccct ccgtgaccaa acaacttaac gtcatcaaac gcttcctgca tgaagccagc
    21421 gaaatcgttc acgccgggga cccggatcgt gaagggcaat tgctggtgga tgaagtgctg
    21481 gactatctgc aactggcacc ggaaaagcgc cagcaggtgc agcgttgctt gataaacgac
    21541 ctgaacccgc aggcggttga gcgggcgatc gaccgtcttc gttccaacag tgagtttgtg
    21601 ccattgtgcg tttctgcgct ggcgcgagcg cgtgccgact ggctgtacgg catcaatatg
    21661 acccgtgcgt ataccattct cggtcgcaat gccggttatc agggcgtact ttccgtggga
    21721 cgcgtgcaga cacccgtgct cgggctggtg gtgcgccgcg atgaagagat tgaaaacttc
    21781 gtggcgaaag acttctttga agtcaaagca catatcgtga cacctgccga tgagcggttt
    21841 accgctatct ggcaaccgag cgaagcgtgt gaaccgtacc aggatgaaga agggcgcttg
    21901 ttacatcgtc cactggcgga gcatgtggtt aaccgcatta gtggtcaacc ggctattgtc
    21961 accagctata acgataaacg ggaatcagaa tccgcgccgc tgccgttttc gctttcggcg
    22021 ttgcagattg aagcggcaaa acgcttcggt ctgagcgcgc agaacgtgct tgatatctgc
    22081 cagaagctgt acgaaacgca caagctaatc acttatccgc gttccgattg tcgctatttg
    22141 ccagaagaac attttgccgg acgccacgcg gtgatgaatg ccatcagcgt tcatgcaccg
    22201 gatctgttgc cgcagccagt ggtagatcca gatatacgca accgctgttg ggatgacaaa
    22261 aaggtcgatg cgcaccacgc catcatcccg accgcacgga gttctgcgat caacctgacg
    22321 gagaacgaag cgaaggtcta taacctgatt gcccgtcaat acctgatgca gttctgcccg
    22381 gatgcggtgt tccgcaagtg tgttatcgaa ctggacattg ccaaaggcaa atttgtcgct
    22441 aaagcccgtt ttcttgctga agcaggctgg cgcacgctgt taggcagcaa agagcgcgat
    22501 gaagaaaacg acggcacgcc attgcctgtg gtggcgaaag gcgatgagtt gctgtgtgaa
    22561 aaaggtgaag tggtagagcg gcaaacccag ccgccgcgcc attttaccga tgcaacactg
    22621 ctttcggcga tgaccgggat cgcgcgcttt gtgcaggaca aagatctgaa aaaggtcctt
    22681 cgtgcgaccg atggtctggg gacagaagca acccgtgccg ggattattga actgctgttc
    22741 aagcgtggtt ttctgaccaa aaaagggcgc tatatccact ccaccgacgc cggaaaagcg
    22801 ttattccatt cgctgccaga aatggcgacg cgaccggaca tgaccgcgca ctgggaatcg
    22861 gtgctgacgc aaatcagcga aaagcagtgt cgctatcagg actttatgca gccgctggtg
    22921 gggacgctat atcagcttat tgatcaagcc aaacgtacgc cggtgcggca gtttcgcggc
    22981 attgtggctc cgggcagtgt tggcagtgct gataagaaaa aggctgcacc gcgtaaacgt
    23041 agtgcgaaaa aaagtccgcc agcagatgaa gccggaagcg gggcgatagc gtaagcgagc
    23101 gaatctttcg tgctattcga gtcatattct gaaatatcca gcggatcaag aaaattcgtt
    23161 ggatattttt ttgcatggat aaaattatcg cctctaaagt atgtaataac agggaatgtg
    23221 atgaaaaaag ttttattgca aaaccatcct gggagcgaga agtattcttt taatggctgg
    23281 ggaatattta atagtaattt tgaacgggtg attaaagaaa ataaggccat gctgctttgt
    23341 aagtgggggt tttatttaac atgtgttgtc gctgtaatgt ttgtattcgc agcgataaca
    23401 tccaacggtt tgaatgaaag aggcctgatt accgcgggat gctcttttct ttatctatta
    23461 attatgatgg ggcttattgt tcgggccggt tttaaagcaa aaaaagaaca actgcattat
    23521 tatcaggcta aaggtattga gccgctcagt atcgagaagt tacaggcgct acaattgatc
    23581 gcaccttatc gattctatca taagcaatgg tctgaaacgc tagagttctg gccgcgaaag
    23641 cctgaacctg gcaaagatac cttccaatat catgtgcttc cttttgactc gatcgatatc
    23701 atcagtaaaa gacgcgagtc tttagagaat caatggggta tcgaagatag cgaaagttat
    23761 tgtgccttaa tggagcattt tctttctggc gaccatggag ccaatacctt taaagcaaac
    23821 atggaggaag ccccagagca ggttatcgcc ttgttgaata aatttgctgt ttttccctca
    23881 gactatatct ctgattgcgc taataatcgc tccggtaaat cctcggcgaa gctaatatgg
    23941 gcggcggaat tatcatggat gatctcgata tcaagcacag cttttcaaaa cgggacaatt
    24001 gaagaagaac tggcctggca ttatataatg cttgcttctc gaaaggcgca cgagttgttc
    24061 gaaagcgaag aagattatca aaaaaatagt ctaatgggat ttctttactg gcatatctgc
    24121 tgctatcgca gaaagttaac ggatgctgaa cttgaagcgt gttatcgtta cgacaagcag
    24181 ttttgggagc actacagtaa gaaatgccgt tggcccataa gaaatgttcc ctggggagca
    24241 tcatccgtta aatactcata aacgcctgaa attttgccgg gggcgctgcg cttgaccggc
    24301 ctacattaga gcgtaactgc aatgaattgt gcccatctgt aggcctgata agcgtagcgc
    24361 catcaggcat ttacaactta aatcacaccc tgcgccagca tcgcatcggc aaccttcaca
    24421 aaaccggcaa tgttcgcgcc ctgcacgtag ttggtttgct caccttcacc accatgctca
    24481 acacaggcat ggtggatatc cagcatgatg tgatgcaaac gtgcgtcaac tttctcggct
    24541 ttccagccca ggcgcgcagc gttttgtgcc atttccaggc ccgacgttgc tacaccgcca
    24601 gcattagccg ctttacccgg tgcaaacagt acgcctgcct gctggaacag ttcagtcgct
    24661 tcgatggtgg tcggcatatt tgccccttca gcgacggctt taacgccatt ggcgataagc
    24721 tgatgcgcgg
//
LOCUS       DAJTOW010000055        24183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTOW010000055 DAJTOW010000000
VERSION     DAJTOW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.055"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(22..594)
                     /locus_tag="OO244_002349"
     CDS             complement(22..594)
                     /locus_tag="OO244_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5664143.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO244_002350"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO244_002350"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5664144.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO244_002351"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO244_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5664145.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO244_002352"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO244_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5664146.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO244_002353"
     CDS             complement(3556..5337)
                     /locus_tag="OO244_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5664147.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO244_002354"
     CDS             complement(5330..7102)
                     /locus_tag="OO244_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5664148.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO244_002355"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO244_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5664149.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO244_002356"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO244_002356"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5664150.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO244_002357"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO244_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO244_002358"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO244_002358"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5664151.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO244_002359"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO244_002359"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5664152.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO244_002360"
     CDS             complement(11658..12029)
                     /locus_tag="OO244_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5664153.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO244_002361"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO244_002361"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5664154.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO244_002362"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO244_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5664155.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO244_002363"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO244_002363"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5664156.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO244_002364"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO244_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5664157.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO244_002365"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO244_002365"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5664158.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO244_002366"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO244_002366"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5664159.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO244_002367"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO244_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5664160.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO244_002368"
     CDS             21800..22378
                     /locus_tag="OO244_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5664161.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO244_002369"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO244_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5664162.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6663 c         5828 g         6026 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gataaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataatg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctgggc gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgcccg ccaagactcc ttctgcactc cccttttgtt
    24181 ata
//
LOCUS       DAJTOW010000056        24079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTOW010000056 DAJTOW010000000
VERSION     DAJTOW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.056"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..168
                     /gene="yjbD"
                     /locus_tag="OO244_002370"
     CDS             <1..168
                     /gene="yjbD"
                     /locus_tag="OO244_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5664163.1"
                     /translation="NSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFS
                     WSANTSTRGRR"
     gene            complement(301..1173)
                     /gene="rluF"
                     /locus_tag="OO244_002371"
     CDS             complement(301..1173)
                     /gene="rluF"
                     /locus_tag="OO244_002371"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5664164.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1521..2468
                     /locus_tag="OO244_002372"
     CDS             1521..2468
                     /locus_tag="OO244_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5664165.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2538..3344
                     /locus_tag="OO244_002373"
     CDS             2538..3344
                     /locus_tag="OO244_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5664166.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3354..3761
                     /locus_tag="OO244_002374"
     CDS             3354..3761
                     /locus_tag="OO244_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5664167.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3761..4255
                     /locus_tag="OO244_002375"
     CDS             3761..4255
                     /locus_tag="OO244_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5664168.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNGEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4321..5118
                     /locus_tag="OO244_002376"
     CDS             4321..5118
                     /locus_tag="OO244_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5664169.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5129..5917
                     /locus_tag="OO244_002377"
     CDS             5129..5917
                     /locus_tag="OO244_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5664170.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5969..7198
                     /gene="sorE"
                     /locus_tag="OO244_002378"
     CDS             5969..7198
                     /gene="sorE"
                     /locus_tag="OO244_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5664171.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7580..8269
                     /gene="pepE"
                     /locus_tag="OO244_002379"
     CDS             7580..8269
                     /gene="pepE"
                     /locus_tag="OO244_002379"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5664172.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8360..9991)
                     /locus_tag="OO244_002380"
     CDS             complement(8360..9991)
                     /locus_tag="OO244_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5664173.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10211..13894)
                     /gene="metH"
                     /locus_tag="OO244_002381"
     CDS             complement(10211..13894)
                     /gene="metH"
                     /locus_tag="OO244_002381"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5664174.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14094..14918
                     /gene="iclR"
                     /locus_tag="OO244_002382"
     CDS             14094..14918
                     /gene="iclR"
                     /locus_tag="OO244_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5664175.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15235..17419
                     /gene="espL4"
                     /locus_tag="OO244_002383"
                     /pseudo
     CDS             15235..17419
                     /gene="espL4"
                     /locus_tag="OO244_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17388..19125)
                     /gene="aceK"
                     /locus_tag="OO244_002384"
                     /pseudo
     CDS             complement(17388..19125)
                     /gene="aceK"
                     /locus_tag="OO244_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19309..20613)
                     /gene="aceA"
                     /locus_tag="OO244_002385"
     CDS             complement(19309..20613)
                     /gene="aceA"
                     /locus_tag="OO244_002385"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5664176.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20643..22244)
                     /gene="aceB"
                     /locus_tag="OO244_002386"
                     /pseudo
     CDS             complement(20643..22244)
                     /gene="aceB"
                     /locus_tag="OO244_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22513..23442)
                     /gene="metA"
                     /locus_tag="OO244_002387"
     CDS             complement(22513..23442)
                     /gene="metA"
                     /locus_tag="OO244_002387"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5664177.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23599..24042
                     /locus_tag="OO244_002388"
     CDS             23599..24042
                     /locus_tag="OO244_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5664178.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5941 a         6143 c         6105 g         5890 t
ORIGIN      
        1 aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag
       61 gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa
      121 gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac
      181 gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc
      241 gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa
      301 tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg
      361 accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt
      421 cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag
      481 gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct
      541 gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat
      601 gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa acggcgcttc
      661 ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc
      721 acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc
      781 gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc
      841 ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc
      901 gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag
      961 ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac
     1021 aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt
     1081 gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta
     1141 tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata
     1201 ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct
     1261 gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat
     1321 aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga
     1381 aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa atcgctttga
     1441 aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg
     1501 tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg
     1561 cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt
     1621 accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg gtcaccatcg
     1681 ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg
     1741 gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga
     1801 tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg
     1861 gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat
     1921 cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct
     1981 atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg
     2041 cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc
     2101 actttaaaac catctctgcc tactgggata atctggatat cgccctggtg ggaattggct
     2161 caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg
     2221 acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat attcacggcg
     2281 caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat aaattaaagc
     2341 aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga attattggtg
     2401 cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct gaactattac
     2461 tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc cctattatct
     2521 aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca
     2581 ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcga
     2641 atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc
     2701 agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta gcggaaatta
     2761 tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc
     2821 tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa
     2881 aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg gcgcgacaga
     2941 tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg ctggaaggct
     3001 cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc acgcgctcct
     3061 ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc gggattctgg
     3121 aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg cgcaatatca
     3181 ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa
     3241 gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc agctacatca
     3301 ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca
     3361 acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg
     3421 tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac gccggagaca
     3481 ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg
     3541 tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg cgcaatcctc
     3601 gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagg
     3661 acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg
     3721 ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta cgctcgcccg
     3781 cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag tggctaacgc
     3841 ccagcgcatt attatctgca atggcgaagt ttataacgat gaagtgcgcc ggacattatt
     3901 gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc
     3961 ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca
     4021 ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata ttggcggcat
     4081 ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat
     4141 taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg tcgtagcgtc
     4201 agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat
     4261 agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag gtgacaaatc
     4321 atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg
     4381 ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt
     4441 ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga attgatagct
     4501 ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc
     4561 tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg
     4621 cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac cgtagtgttc
     4681 cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga tattctgcat
     4741 gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat tgtctcactg
     4801 ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt tgtgacccga
     4861 ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg
     4921 atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc
     4981 gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag
     5041 ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt
     5101 gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct
     5161 ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca
     5221 cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc cattaaaaga
     5281 ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc cagccgtatg
     5341 tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca aagtcggtct
     5401 gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc gcccgattac
     5461 cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc tgctgttctt
     5521 ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc tcggctttcg
     5581 caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac tcaccgaagg
     5641 cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga cgtcaatcaa
     5701 cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca ccatgaccgt
     5761 gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga cgctactaat
     5821 ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc tgtttggctt
     5881 agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg cacgactgcc
     5941 gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc tttatggtaa
     6001 acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg aaatcctcgc
     6061 aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc tgggtgaaaa
     6121 ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc acgaattttg
     6181 cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg gtcagcgtta
     6241 tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct actccttccc
     6301 gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg aacaatattg
     6361 cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac cgctttcctg
     6421 cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata accacacgat
     6481 ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa tgggactgtt
     6541 ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca ttaccgatac
     6601 cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc aaacactgat
     6661 tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga gtggcggtca
     6721 cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc tcgcctcttc
     6781 cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata aacatttcag
     6841 cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg gcacgtcagg
     6901 cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa aagtgcaggc
     6961 cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca cgcttgaatt
     7021 gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc cgctgacgtc
     7081 actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc aggggatctg
     7141 gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc atgattaatc
     7201 gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg accggatgtg
     7261 cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc agcgccttaa
     7321 ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga atgaagcctt
     7381 attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag gccaggtaag
     7441 cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg caatacgtga
     7501 catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct tccgtgattt
     7561 cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa
     7621 agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc
     7681 ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag agaaaacggc
     7741 tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt
     7801 tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt tccagttgct
     7861 gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta aacgtggcgc
     7921 gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa
     7981 cgatatgccg attgtcgatc cgcaaggttt cgatgctcta aatctgttcc cgttgcaaat
     8041 caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg
     8101 tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc cggaaggtaa
     8161 ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca cttatgtgtt
     8221 taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag tgaattgccg
     8281 gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc gtcttatcag
     8341 gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc ggctgttcca
     8401 gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct cccagtaagc
     8461 ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg cgatcgacgt
     8521 gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga cgcaaacgac
     8581 gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa ttactgagca
     8641 attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc cgccgtgctg
     8701 ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag gcctgttcaa
     8761 ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc gccaactctt
     8821 cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg atatcatctg
     8881 ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta ttcagccctt
     8941 ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag
     9001 caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc tgggtatcca
     9061 gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca
     9121 gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt
     9181 ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca
     9241 ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg
     9301 cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca atcaccagac
     9361 agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc agcagtacag
     9421 cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca tccagcagaa
     9481 tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta
     9541 cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata ccgacgcggc
     9601 ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa
     9661 gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga
     9721 catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacga
     9781 acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta acgccgatcc
     9841 ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta cgcaaacgcg
     9901 cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc agcagggcga
     9961 cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat cgttatatgt
    10021 tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc tttactgtgc
    10081 cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta taagtgtttc
    10141 atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca
    10201 gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacatcgg
    10261 agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga atttgcgcca
    10321 ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat gcaccaggcc
    10381 acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc agcagctccc
    10441 agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc ggacggatgc
    10501 cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc
    10561 agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg
    10621 caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca
    10681 gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag
    10741 aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct
    10801 gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca cggtagattt
    10861 caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg
    10921 ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct
    10981 caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc gaccaggtca
    11041 taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct tccacttcct
    11101 gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat gcgaaatcgt
    11161 tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc ccgtgctgaa
    11221 tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc tgagtatcgg
    11281 aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac accgtagggc
    11341 cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc gcgccgccaa
    11401 tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt
    11461 ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact tctttggcga
    11521 tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac
    11581 attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct ttcacggtgg
    11641 cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata aacggttcga
    11701 gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc aggaacattt
    11761 tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg ccttcaatca
    11821 cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg ataaactcgg
    11881 ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag cgccactccg
    11941 cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg gcaagctcca
    12001 gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc gcgtcgcgca
    12061 gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc cccatatcca
    12121 tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc accggatcgt
    12181 tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg tgcggcagtt
    12241 cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa
    12301 taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa
    12361 cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgagtat
    12421 cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag cgacgcaaca
    12481 atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc
    12541 ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag gagtcgatca
    12601 taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg
    12661 ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc gcgccgtttt
    12721 ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg atcaggcgct
    12781 tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac aggctatcgt
    12841 cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt tccggcagtt
    12901 tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc ggcgtggtcc
    12961 cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt atctgttttg
    13021 ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg gcgttcgggt
    13081 gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg cgcagttcat
    13141 cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc aatgagttgt
    13201 aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg
    13261 agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaagaccg
    13321 ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcaccacctt
    13381 ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa gtgatgttgc
    13441 ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt gggccaagaa
    13501 caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca gcacaggcgc
    13561 gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg
    13621 ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa
    13681 agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc aggtcgttgt
    13741 tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca
    13801 gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata cgttcattta
    13861 actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg cttaacccag
    13921 atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa aagcgaccac
    13981 cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt
    14041 agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct gtcatggtcg
    14101 cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac
    14161 aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca
    14221 gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg acccaccgcc
    14281 tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg ggacactggg
    14341 caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag
    14401 caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg gtcaatatgg
    14461 cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacccacc
    14521 tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta
    14581 aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt
    14641 tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa gatctcgccc
    14701 aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg ctacgttgcc
    14761 ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct atttccggac
    14821 cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg attaaagcag
    14881 cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg ggcagaggca
    14941 atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa tcgcattgac
    15001 gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca ttatttttaa
    15061 acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga aacttttacg
    15121 gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat attaaatttt
    15181 ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat ttaaatgatt
    15241 actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca gacaaatgaa
    15301 caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc tggcaaggag
    15361 ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt tcgtcatatc
    15421 tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc tgataagaaa
    15481 gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat tgctacaata
    15541 tttgaagaat acctaatgga aattttacct caacgctcag acactcttga taaacaagaa
    15601 ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact taatggtcaa
    15661 tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct gcacaatata
    15721 ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac tcttcagctg
    15781 aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg ttgatttcca
    15841 gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt atgaaagcat
    15901 attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta ctggtggata
    15961 accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt acccattacg
    16021 tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg agcgaaagca
    16081 aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat agcctcctga
    16141 aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca cttccgaaag
    16201 aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta tcaccagcaa
    16261 cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg atgattcagc
    16321 tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat agtattaacc
    16381 tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag actattttca
    16441 attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc atgcatattc
    16501 tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca cgtaaggata
    16561 aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca catcatttag
    16621 ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc catgttttat
    16681 accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc gcgttacctt
    16741 tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg aaggcaaagg
    16801 atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc gatatcgtga
    16861 aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca gcttccgata
    16921 ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt atggccatgc
    16981 agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact ctttttaata
    17041 cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata
    17101 tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga cggtttatct
    17161 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
    17221 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg
    17281 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
    17341 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
    17401 tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc
    17461 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
    17521 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
    17581 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
    17641 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
    17701 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
    17761 gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
    17821 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg
    17881 attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc
    17941 ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca caaagttttc
    18001 aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca gttgatagca
    18061 ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt
    18121 gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac gaatacccgg
    18181 cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc
    18241 ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg
    18301 cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata
    18361 agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat
    18421 aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg
    18481 cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac
    18541 ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat
    18601 gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac
    18661 gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcta gcgggcgggg
    18721 aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag
    18781 cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa
    18841 gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc
    18901 gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg
    18961 atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc
    19021 gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc
    19081 ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc gccctcatca
    19141 ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt
    19201 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc
    19261 ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt agaactgcga
    19321 ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt
    19381 cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg tatagccatc
    19441 tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca taccttcgcc
    19501 ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga tacctgctag
    19561 ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc tggcaatagt
    19621 tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag ccagcagttt
    19681 gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca gatccggcgt
    19741 ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac ggctgatcgc
    19801 ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc cggtaataaa
    19861 ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag catcggtacg
    19921 tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg cgaccagttt
    19981 ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc atttcttcac
    20041 tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca tcgctttcat
    20101 cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa cgatcggcag
    20161 gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt ggatctgatc
    20221 ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt ttgccggata
    20281 gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta cctgccatcc
    20341 cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac cgccagtcag
    20401 tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac gccacatttt
    20461 cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta atttcaccac
    20521 atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc actctttctg
    20581 taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag ttatgtggtg
    20641 gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc atcggaagtg
    20701 gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa acgttcttcg
    20761 cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa caaggctttg
    20821 gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca gatcgaggta
    20881 cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac gcagccgttg
    20941 ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg cataccttct
    21001 tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt
    21061 tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa taccgccatt
    21121 gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt attggcttcc
    21181 agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc atctttgctc
    21241 ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca ggttttaatc
    21301 agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg tctgtctggc
    21361 aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta atcccagcga
    21421 ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc catctggaac
    21481 acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag
    21541 cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta ggactgggtt
    21601 ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata gttgtggaag
    21661 aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca ggtgacatgt
    21721 ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt gggcttgagc
    21781 tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc atcgcgcagg
    21841 ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc gaaatcggcc
    21901 ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc taccgggcca
    21961 gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt ccaatcagca
    22021 tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc aatatcttgc
    22081 tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc
    22141 tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc gccatacggc
    22201 cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc
    22261 atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt cgttcagcac
    22321 aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta attaaaaata
    22381 cttgataact tattgataac aatatgaata aaatttattc acttgctgag gtgcgtttcg
    22441 gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac
    22501 tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg gcgtgatctg
    22561 atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac gccagctcgc
    22621 tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat ccgggtctag
    22681 tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat attcgggatg
    22741 gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat ccccttcttc
    22801 cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg gaaagtcagc
    22861 atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg
    22921 atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat
    22981 gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg tcgaggtgac
    23041 gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga catcattaaa
    23101 ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct gttcctgaat
    23161 atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc gcgattcacg
    23221 ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa gcaggcgcag
    23281 aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa ccttaagtgg
    23341 acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt cttcacgcaa
    23401 gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat tacctcacta
    23461 catacgctta tacgtttaga catccagata gctgaagata accagaaaat ttgccaatgt
    23521 cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag aatataagaa
    23581 tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga ggaaggggag
    23641 gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt atcagcagag
    23701 tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc gttgtgggtc
    23761 gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca gcatatggat
    23821 gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct ggtggagcat
    23881 gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga gcaggcggtt
    23941 gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga cgatttgggg
    24001 aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc tgccgtgcct
    24061 gcccaagctg gagaagacg
//
LOCUS       DAJTOW010000057        24026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTOW010000057 DAJTOW010000000
VERSION     DAJTOW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.057"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            642..2570
                     /gene="thrS"
                     /locus_tag="OO244_002389"
     CDS             642..2570
                     /gene="thrS"
                     /locus_tag="OO244_002389"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5664179.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2574..3116
                     /gene="infC"
                     /locus_tag="OO244_002390"
     CDS             2574..3116
                     /gene="infC"
                     /locus_tag="OO244_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5664180.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3213..3410
                     /gene="rpmI"
                     /locus_tag="OO244_002391"
     CDS             3213..3410
                     /gene="rpmI"
                     /locus_tag="OO244_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5664181.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3463..3819
                     /gene="rplT"
                     /locus_tag="OO244_002392"
     CDS             3463..3819
                     /gene="rplT"
                     /locus_tag="OO244_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5664182.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3936..4060
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3942..3986
                     /gene="pheM"
                     /locus_tag="OO244_002393"
     CDS             3942..3986
                     /gene="pheM"
                     /locus_tag="OO244_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5664183.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4269..5252
                     /gene="pheS"
                     /locus_tag="OO244_002394"
     CDS             4269..5252
                     /gene="pheS"
                     /locus_tag="OO244_002394"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5664184.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5267..7654
                     /gene="pheT"
                     /locus_tag="OO244_002395"
     CDS             5267..7654
                     /gene="pheT"
                     /locus_tag="OO244_002395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5664185.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7659..7958
                     /gene="ihfA"
                     /locus_tag="OO244_002396"
     CDS             7659..7958
                     /gene="ihfA"
                     /locus_tag="OO244_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5664186.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8059..9039
                     /gene="btuC"
                     /locus_tag="OO244_002397"
     CDS             8059..9039
                     /gene="btuC"
                     /locus_tag="OO244_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5664187.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9102..9653
                     /gene="btuE"
                     /locus_tag="OO244_002398"
     CDS             9102..9653
                     /gene="btuE"
                     /locus_tag="OO244_002398"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5664188.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9653..10402
                     /gene="btuD"
                     /locus_tag="OO244_002399"
     CDS             9653..10402
                     /gene="btuD"
                     /locus_tag="OO244_002399"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5664189.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10480..10944
                     /locus_tag="OO244_002400"
     CDS             10480..10944
                     /locus_tag="OO244_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5664190.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11191..11904
                     /gene="ydiV"
                     /locus_tag="OO244_002401"
     CDS             11191..11904
                     /gene="ydiV"
                     /locus_tag="OO244_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5664191.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11968..13404
                     /locus_tag="OO244_002402"
     CDS             11968..13404
                     /locus_tag="OO244_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5664192.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13408..13599)
                     /gene="hemP"
                     /locus_tag="OO244_002403"
     CDS             complement(13408..13599)
                     /gene="hemP"
                     /locus_tag="OO244_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5664193.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13731..14777)
                     /gene="aroH"
                     /locus_tag="OO244_002404"
     CDS             complement(13731..14777)
                     /gene="aroH"
                     /locus_tag="OO244_002404"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5664194.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14934..15767)
                     /gene="ppsR"
                     /locus_tag="OO244_002405"
     CDS             complement(14934..15767)
                     /gene="ppsR"
                     /locus_tag="OO244_002405"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5664195.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIHPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16099..18477
                     /gene="ppsA"
                     /locus_tag="OO244_002406"
     CDS             16099..18477
                     /gene="ppsA"
                     /locus_tag="OO244_002406"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5664196.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18534..20180)
                     /gene="fadK"
                     /locus_tag="OO244_002407"
     CDS             complement(18534..20180)
                     /gene="fadK"
                     /locus_tag="OO244_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5664197.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20237..20530)
                     /locus_tag="OO244_002408"
     CDS             complement(20237..20530)
                     /locus_tag="OO244_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5664198.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20527..21816)
                     /locus_tag="OO244_002409"
     CDS             complement(20527..21816)
                     /locus_tag="OO244_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5664199.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21872..22810)
                     /locus_tag="OO244_002410"
     CDS             complement(21872..22810)
                     /locus_tag="OO244_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5664200.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22830..23594)
                     /locus_tag="OO244_002411"
     CDS             complement(22830..23594)
                     /locus_tag="OO244_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5664201.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5926 a         5842 c         6326 g         5932 t
ORIGIN      
        1 tgagctggcg cgcaaattta ttaataggct ctgatgaagg tgttggtatg tcgctatcga
       61 tgtgcaatct atgaggagac ttataatgag ttctgataat gatatcgttt tgactcataa
      121 tctattcgcc ttatatatat ctggaggttt tgaggatgat gattaattat tatcgccaga
      181 acgaaatgtg cacgaaaaat tgttataaga attgtgtcac ttttatcatt gtgatatgac
      241 cacttcgtca ttaatctcat gagcgtttaa aatactaccg ccaaatcacg caacgaactt
      301 tgctggattc agtttatgct gctgtaaatc cgctcgcagt aaacctttca gacgcaccgt
      361 gatgttatca gtggttcttt aagcgttttg ctggtgtact cactacaaac gaattgcgaa
      421 tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa catcgttcaa
      481 ccgggtggaa ggtgagaggt taatgtcttt catttgtgta cctgattgcg aaccaattta
      541 gcatttgttg gctagatggt ttcgcaatga actgttaata aacaaatttt tctttgtatg
      601 tgatctttcg tgtgggtcac cactgcaaat aaggatataa aatgcctgtt ataactcttc
      661 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
      721 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
      781 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
      841 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
      901 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
      961 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1021 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1081 ctttcgccaa ccgtggggag agctacaaag tttccattct tgacgaaaac atcgcccatg
     1141 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1201 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1261 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1321 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1381 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1441 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1501 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1561 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     1621 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     1681 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     1741 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     1801 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     1861 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     1921 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     1981 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2041 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2101 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2161 cggtaatgat tcaccgcgca attctggggt cgatggagcg tttcatcggt atcctgaccg
     2221 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2281 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2341 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2401 tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2461 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2521 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     2581 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     2641 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     2701 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     2761 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     2821 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctagtacag
     2881 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     2941 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3001 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtagtcgaa tccttcccaa
     3061 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3121 ttcaagtagc actatctgtg gagcctttgg gcttcacagg cattgttcgc ctttgtttcg
     3181 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3241 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3301 gtcacattct gactaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3361 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3421 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3481 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3541 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     3601 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     3661 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     3721 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     3781 accactctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     3841 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     3901 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     3961 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4021 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4081 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag tgcggagaaa
     4141 ccggagcgta cttaagtacg tgaggatttc gagcactgcc cgggaccaaa atggcaagta
     4201 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4261 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4321 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4381 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4441 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4501 tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac gattgatgtc tctctgccag
     4561 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     4621 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     4681 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     4741 tctggtttga cgctacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     4801 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgtgtttat cgtaacgact
     4861 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     4921 tcagctttac caacctgaaa ggcacgctgc acgacttcct gcgtaacttc tttgaggaag
     4981 atttgcagat tcgcttccgt ccttcctact tcccgtttac cgagccttct gcagaagttg
     5041 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ctgcgggatg gtgcatccga
     5101 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5161 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5221 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5281 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatacgctgg cgaatcaaat
     5341 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5401 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5461 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgcggtgcgc caaactgccg
     5521 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     5581 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tttctgagct
     5641 gggaatttct gacgatcaca gtggcattat cgaactgcct gcggatgcgc cgattggcac
     5701 cgacatccgc gaatacctga aactcgatga taacaccatc gaaatcagcg tgacaccaaa
     5761 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     5821 gccgctggtt gaaccggaaa tcgttccggt tggtgcgact atcgacgaca cgttgccgat
     5881 tacagtcgaa gcgccggaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     5941 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6001 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6061 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6121 aacgctggtg ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6181 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6241 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6301 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6361 tccggtactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6421 tggtgaggct ggcccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6481 catcactcta cgtcgtagca aactggatcg cctgataggc catcatattg ctgatgagca
     6541 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgaatggca
     6601 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg tcgaagaagt
     6661 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     6721 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     6781 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     6841 tccaggcgtt gaagccttac tgctggcaag cccgatctct gttgaaatgt cagcaatgcg
     6901 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     6961 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7021 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7081 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgaccttg aatccgttct
     7141 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7201 tccggggcaa tccgcagcga tttatctgaa aggtgaacgt attggttttg ttggagttgt
     7261 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7321 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7381 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7441 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7501 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7561 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     7621 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     7681 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     7741 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     7801 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     7861 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     7921 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     7981 ctatgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8041 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8101 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8161 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8221 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8281 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8341 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8401 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8461 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8521 atctgcagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     8581 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     8641 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     8701 gcacttggcg agatctcggc gcggcaactg ggtttaccca tgtggttctg gcgcaatgtg
     8761 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     8821 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     8881 ttacttcccg gctgcgcgct ggcaggggcg agcgcattgc tgctggccga tattgtagcg
     8941 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9001 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9061 ttaaaagcta agatttttac tgaccacacc taggagaaac gatgcaagat tccattctga
     9121 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9181 tgctgttaat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9241 agaatattca aaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9301 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9361 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga cgccatccgc
     9421 tgtatcaaaa attgattgcc gcagcgccga ccacagtcgc gccggaagag agcggattct
     9481 atgcccgtat ggtcagcaaa ggccgtgcac cgctgtaccc ggatgatatt ttatggaatt
     9541 ttgaaaaatt cctggttggc agggacggaa aagtcatcca gcgtttttcc ccggatatga
     9601 cgccggaaga tcccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
     9661 tgtgatgcag ttacaggatg ttgcggaatc tacccgcctg gggccgcttt caggcgaggt
     9721 tcgggctggg gaaatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
     9781 ggcgcgaatg gccggaatga ccagcggtaa gggaagcatt cagttcgcgg ggcaaccact
     9841 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
     9901 gccgccgttt gcaatgccgg tctggcacta cctgacactg catcagcacg ataaaacgcg
     9961 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggtcgtag
    10021 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgttgca
    10081 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10141 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgcgctgt gtcagcaagg
    10201 actggcgatt gtgatgagca gtcacgatct caaccacacg ttgcgtcatg cgcatcgggc
    10261 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10321 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10381 aatgctgatt tcgaccatct gatttcatta gtatcccgaa aatcgggtaa ttacgacgct
    10441 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10501 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10561 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    10621 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    10681 gtgacgatgc gcgatcgttt cgatttgcat ctgccccgtg aaaccaaaca acaagcctct
    10741 attggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    10801 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    10861 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    10921 ttctggcagg cgagacgaat ctagtatgtc gaaaaattga cggcgatgat acatcgccgt
    10981 caatttactc ctgaatacag tagtattttg cagacaaaaa ttccgtaaat ggattattcg
    11041 tttataatca ggataatcta aaaaaaggcc ccctgaaaaa gtggttaaca tggaacaaat
    11101 gaaagttact ccttctgtaa ttgttatgtc ttatggcctc atccctttta gccggatact
    11161 gaaaaacatc cttcgagagg gacggttacc atgaagattt ttttggagaa tctttatcat
    11221 tctgattgct atttcctgcc gataagagat aaccagcaac tccttgtcgg cgttgagtta
    11281 attacccatt tctccagtga agatggcacg gttcgtatcc cgacgagtcg ggttatagcg
    11341 caacttaccg aggagcaaca ttggcaatta ttttcagagc agttggaatt actaaaatca
    11401 tgccaacact tttttattca acataaactc tttgcctggc ttaatttaac gccacaagtg
    11461 gctacattat tattagacag ggataattac gcaggcgaac tattaaaata tccgtttatt
    11521 gagctgttga ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa tcgcgatctt
    11581 ttgtccttat cgcaaatgta tccccttgtg ttaggcaatt tgggagcagg taatagcaca
    11641 atgaaagcgg tttttgatgg tcttttcacc cgcgtaatgc tggagaaagg ttttattcag
    11701 caacaaatca cgcatcgctc cttcgaaccg tttattcgtg cgatccaggc gcaaatttcc
    11761 ccttgttgca attgcatcat cgcggggggc attgatacgc cagaaatatt ggcacagatt
    11821 acccccttcg atttccatgc tctccaggga tgcctgtggc ctgccgttcc tataaatcag
    11881 ataacgacgt tggttcagcg ataaccctcc tgtttgctgg tgtttaagac gagagtaacc
    11941 gtctacacta tcaaacagat aggatctatg accctgtctt ttattacccg ctggcgcgat
    12001 gaattgccag aaacctatac agcactttcc cctacgcctt taaataatgc ccggctgatt
    12061 tggcataata ccgaactcgc taacacgctg agtattccat cgtcgctgtt taaaaatgcc
    12121 gcaggtgtct ggggcggcga aaccctactg cctggtatgt caccactggc acaggtttac
    12181 agtggtcatc agttcgtcgt ctgggcgggc caactgggtg atgggcgcgg cattttactt
    12241 ggcgaacaac tgcttgctga tggcactaca atggactggc atctgaaagg tgctggcctg
    12301 acgccttatt cgcgaatggg tgatggacgg gcggttttac gttcgacgat acgagaaagt
    12361 ctcgccagtg aggcgatgca ttatctgggc attccgacga cccgcgcgtt aagtatcgtc
    12421 accagcgatt cgccagtgta tcgggaaacg gtggagccag gcgcgatgct gatgcgtgtg
    12481 gcaccaagtc atctgcgctt tggtcatttc gaacattttt actatcgccg cgagccggaa
    12541 aaggttcgtc agttggctga ctttgccatt cgtcattact ggtcacatct tgaagacgat
    12601 gaggacaaat accgtctctg gtttaacgat gttgtcgcac gtaccgcgtc gttaattgcc
    12661 caatggcaga cggtcggctt tgcacatggg gtgatgaata ccgacaacat gtcgctgctg
    12721 ggactgacgc ttgattacgg gccgtttggt tttcttgatg attacgaacc cggttttatt
    12781 tgtaatcact cggatcatca agggcgttat agctttgata atcaacctgc tgtcgcgttg
    12841 tggaatttac agcgtctggc gcagacattg tcaccatttg ttgccgtaga tgccctgaat
    12901 gaggccctgg acagctatca gcaggttttg ttgacgcatt atggacaacg gatgcggcag
    12961 aaactgggct tcatgacgga gcaaaaagag gataacgcgc tactgaatga attattcagt
    13021 ctgatggcgc gagagcgcag cgattatacc cgcacattcc gcatgctgag tctgaccgag
    13081 cagcacagcg cggcgtcacc gctacgtgag gagtttattg atcgtgcggc atttgatgac
    13141 tggtttgccc gttatcgggg gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag
    13201 caactgatgc aaagcgttaa cccagctctg gtattgcgca actggttggc gcaacgggcg
    13261 attgaagcgg cagaaaaggg tgatatgatg gaattgcacc gcctgcatga ggcgttgcga
    13321 aatcctttca gcgacagaga tgatgactat gtcagtcgtc cacctgactg gggtaaacgg
    13381 ctggaagtca gctgctcgag ttaaaggcta cttagttaac agcagcttgc cagcctgagt
    13441 tttacggagc agatattctt gcccgtcatg atcgataatc agtttgccat ccggacctaa
    13501 cagtgtctgg ctggtaattc gccggatggg ctgcggggaa gcggtcttgt gattggcatc
    13561 cgtttcaggc gtgagttttc tgctatccgt ataacgcata aaaattaata ataattgata
    13621 taacaatgat tcctattatc aatattatgg caggccagag caagcagtaa tgtgttgaga
    13681 agacaacaag ttgcgcagaa atgcccatcc catcaggaat gggcacgcat tcagaagcgg
    13741 gtatctaccg caaaggcgag tttttcgacc aggcgttcgg tatcttccca gcccagacac
    13801 gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct
    13861 tcgcgcagga aactttccgc cataattcca gcaatctccg tagagccatt gcggatttgc
    13921 tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca gttaccgtgg
    13981 ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg
    14041 gcgatatcat cggcatgata attcggtttt ttgccgccac gcataataat gtggccatac
    14101 gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga gaggaacata
    14161 tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc atcggtacca
    14221 tttttaaaac ctaccggaca ggagagtgct gaagccattt cgcggtggat ctgactttcg
    14281 gtagtacgag cgccaatcgc gccccaactg attaaatcag caataaactg accggttacc
    14341 atatcgagga actcagtcgc ggtagggaca cccagctcat ttacctgtaa aagtaatttg
    14401 cgcgccagct ccagaccgtg atttacccga tagctgccgt ttaaatccgg atcggagatt
    14461 agccctttcc agccgacaac agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc
    14521 agccgtgact ggtactggtt gcgcagcgac tgcagacggg tggcgtactc cattgcagcg
    14581 gtgagatcgt ggatcgagca ggggccaata atgaccaaca gtcgcttatc ttcaccattc
    14641 agtatttttt caattctgcg gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg
    14701 ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc agtacggagt
    14761 tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca ggactaccgg
    14821 aaagtgatgc gcatcacgat aaaccaatcg cagctgattt caagtgcgag gtgtgtcaca
    14881 cctcgccggg tggttagcat gataacaaaa aaacaaacca atgcactagt tctctagtac
    14941 attcggcgac taaggcccat gatatcgagg atcttggtgg caatctcttc taccgaataa
    15001 ttggtactgt taatccacgg gatctgattt ttacggtaca aggcttccac ttccgcgact
    15061 tccatcctgc actgacgaag cgaggcatag cgactgttct cccgacgttc ctcgcgaatc
    15121 gccgccagac gttccgggtg gatagtcagg ccgaacaatt tatgctgaag cggtttgagc
    15181 gacgcgggta gcaccagatt atccatatcg tcggcaataa aggggtagtt tgccgcgcgg
    15241 ataccaaatt gcattgccag atacagactg gtgggggttt taccacagcg agaaacaccg
    15301 agcaggatca cctgagcctg atccagattg cgcaacgaaa tgccgtcatc gtgggcgagg
    15361 gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg gttaaggcca
    15421 tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag cggggcaacc
    15481 agcgcctgaa cgatatcctg gcaaaagcct tcactttgca agatgatggc gcgaatctcc
    15541 ggcaacacga tggagtagaa gaccagcggg cgcacgcctg tctggtgata aattgcgtca
    15601 atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag cgtgatgctg
    15661 ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc agttattgcc
    15721 gtaccatcag aaatataaaa aacgtggcga tcaacagcat tatccatttt gttcttcccg
    15781 tgaagcagac ataattctat gaaagcataa attaaaagcg cacagaagcg taggacgtaa
    15841 tgtctggttt ataaaatgaa ccttcatttt attttttata aaaacagcat ttcattttta
    15901 tggtttcgtt tatgccgatg gtttatgtgg aaattgtcga atagagcagg tttgcgcaac
    15961 gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca ccgttttttt
    16021 catccggtta aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac cgttcattta
    16081 tcacaaaagg attgttcgat gtccaacaat ggctcgtcac cgctggtgct ttggtataac
    16141 caactcggca tgaatgatgt agacagggtt gggggcaaaa atgcctccct gggtgaaatg
    16201 attactaatc tttccggaat gggtgtttcc gttccgaatg gtttcgccac aaccgccgac
    16261 gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc gcatttatga actgctggat
    16321 aaaacggata ttgacgatgt tactcagctt gcgaaagcgg gcgcgcaaat ccgccagtgg
    16381 attatcgaca ctcccttcca gcctgagctg gaaaacgcca tacgcgaagc ctatgcacag
    16441 ctttccgccg atgacgaaaa cgcctctttt gcggtacgct cctccgccac cgcagaagac
    16501 atgccggacg cttcctttgc cggtcagcag gaaaccttcc tcaacgttca gggttttgac
    16561 gccgttctcg tggcagtgaa acatgtattt gcttctctgt ttaacgatcg cgccatctct
    16621 tatcgtgtgc accagggtta cgatcaccgt ggcgtggcgc tctccgccgg tgttcagcgg
    16681 atggtgcgct ccgacctcgc atcttctggc gtgatgttct ccattgatac cgaatccggc
    16741 tttgaccagg tggtgtttat cacttccgca tggggccttg gggaaatggt cgtgcagggt
    16801 gcggttaacc cggatgagtt ttacgtgcat aaaccgacac tggcggcgaa tcgcccggct
    16861 atcgtgcgcc gcaccatggg gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag
    16921 cacggcaagc aggttaaaat cgaagacgta ccgcaggaac agcgtgacat cttctcgctg
    16981 accaacgaag aagtgcagga actggcaaaa caggccgtac aaattgagaa acactacggt
    17041 cgcccaatgg atattgagtg ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag
    17101 gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac gctgcattca
    17161 cagggtaaga ttatcgccga aggccgtgct atcggtcatc gcatcggcgc gggtccggtg
    17221 aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac ctggcgacgt gctggttact
    17281 gacatgaccg acccggactg ggaaccgatc atgaagaaag catctgccat cgtcaccaac
    17341 cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg aactgggcat tccggcggta
    17401 gtgggctgtg gagatgcaac agaacggatg aaagacggtg agaacgtcac tgtttcttgt
    17461 gccgaaggtg ataccggtta cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc
    17521 agcgtagaaa cgatgccgga tctgccgttg aaagtgatga tgaacgtcgg taacccggac
    17581 cgtgctttcg acttcgcctg cctgccgagc gaaggcgtgg gccttgcgcg tctggaattt
    17641 atcatcaacc gtatgattgg cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa
    17701 ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt
    17761 tacgttggtc gtctgactga agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc
    17821 gtcattgtcc gtctctctga ttttaaatcg aacgaatatg ccaacctggt cggtggtgag
    17881 cgttacgagc cagatgaaga gaacccgatg ctcggcttcc gtggcgcggg ccgctatgtt
    17941 tccgacagct tccgcgactg cttcgcactg gagtgtgaag cagtgaaacg tgtgcgcaac
    18001 gacatggggc tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg
    18061 aaagcggtgg ttgaagaact ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa
    18121 atcatcatga tgtgtgaaat cccgtccaac gccttgctgg ccgagcagtt cctcgaatat
    18181 ttcgacggct tctcaattgg ctcaaacgat atgacgcagc tggcgctcgg tctggaccgt
    18241 gactccggcg tggtgtctga attgttcgat gagcgcaacg atgcggtgaa agcactgctg
    18301 tcgatggcta tccgtgccgc gaagaaacag ggcaaatatg tcgggatttg cggtcagggt
    18361 ccgtccgacc acgaagactt tgccgcatgg ttgatggaag aggggatcga tagcctgtct
    18421 ctgaacccgg acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat
    18481 aaatccccgg cggcgttcag tcgccggggt tatgtgatcc ccgaagatga aacttattca
    18541 atctcttcac agacatcctg cgttaatcgc cgtataatat cttttcttaa caaaaacttt
    18601 tgtattttat ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat
    18661 ttatattttg cgacccgttt acggctaaaa aaagccgcca tctcttccag cgataatgaa
    18721 tgatgcggcg ctttcagcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc
    18781 attgcaacca cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca
    18841 cggctgctaa tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt
    18901 atatagccag cctcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc
    18961 agcgcacggg cggttaattc aggttcatca aaatacccca taaacacatt ggggccacgc
    19021 gatgcttctt caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta
    19081 atctctacac ctgcggcagc gtaaccatcg gtatgcataa agcgcgacaa agaattatcg
    19141 agattcacca ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg
    19201 ctgcgctgct ggcattcgcg cgccactttt ttggggattg tggtaccgcc gcaaagaaag
    19261 aagcgcagcg ctgaaaggtc tgcgggttgt ttctctagta aattcaaaag atcatagaca
    19321 aacggcgttg cgcctagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca
    19381 tcaggagtga aaatatctaa caacacgctg cgagcgccaa ttaagaatgg tgccgttacg
    19441 ccatgcagaa agcccgttgc gtgaccaagt ggcgcaggca tcataaagac atccagccag
    19501 gtcagattca gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc
    19561 atcacgccct ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg
    19621 ccgtgggtcg ttatcgccgt ggtcagtggg atattgtcgg cgataatctg acttaatgag
    19681 agggaagagg tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc
    19741 tgattttgca gcggcaggat taaatctacc ggacgcgttt gtttaaacaa cgtcggtgca
    19801 aagaacattt ttgcctgaca cttattgagc acccacacca gttctgcttc ccgccaggaa
    19861 ggcaacagcg gcacggaaac cgcaccgatt ttcaggcagg caagatagat aacggtaaat
    19921 tcacaccagc caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac
    19981 atccagtttg ccagacagct cgcggcgtga tcgagcgcgc tataggtgta cgatgcacca
    20041 tgattatcga ccacggcaat tttgtctggc atcgcacgag cggtctgctg ccagtaatcg
    20101 gccagtgaag catcgcccca tagcccttgc tgacgatacg ccgcacgacg ttgttcgtta
    20161 aacgttaatg tcactttcat tttggactct cgaaacagaa catcaggccc gagttgcggg
    20221 cctgtgggat gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt
    20281 gccactgttc gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac
    20341 cggcgggatc aaaatgaatg tttcctacgt catcctgctt ataaagtccg gcagggcagg
    20401 ctttcattaa tttacggaat tcattgatat cgggattttc cgtcagaatg atatgcgggt
    20461 ggccctcatc aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt
    20521 tctggctcat agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat
    20581 tttcttcgcg tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt
    20641 gaacatctcg ttcatgatgt cggctaccat tcgtgggtat tggctaaaca ggcgcgggtt
    20701 ttccatcagc gccgggatct tgcgaaaatg ctgcatatca cgcatgacgc agctttgttc
    20761 cagctcacgt ttgtattgcg ccagactgtt ggcggagaaa tccgcgcgtt ctttggcggc
    20821 gatcaccgtt gtggcggcag cctgagccga tgcaatggct aaatccatgc cgcggaccgt
    20881 aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc cctcgttaac
    20941 cagttgcggc accattgcca gaccgccttc cggcaccata tgcgcggaat attcaagcag
    21001 tttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg
    21061 cggcacgctt ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaaggatat
    21121 ggaatccttg ttggtataga gaaatccccc gcccatcagg ccgtcagaag gggaaccggc
    21181 aaacagccag gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg
    21241 tgtgaggcca ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac
    21301 cattcccagc gagcggccaa gcatcgagtt aacgccatca gccagaatca ccacattcgc
    21361 ttcgagaata tcatccccag cctgcacgcc agtgaccttg tttccttcac gaaccagcgc
    21421 gtcgacgcga acgcctggga taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca
    21481 cgggtccaga cgattacgca atacggtata agatgcgtgt tgaggaacat ctggttgctc
    21541 gcggtgaaaa tcgagggtaa cggcgctttc ttcggttaag aaggagattt tctcgcgtgt
    21601 gaccttgcgt tctaccggcg ctgatactgc aaagcctgga atgattgctt caagtgtgtg
    21661 ggcataaaga cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac
    21721 caggacatcc agcccggctc gtgccatgac cagtgcggca acgctaccag caacgcccgc
    21781 accgaccaca atggcatcaa atttgtcatc cgacatgcgt tatgctccct tcctgctgcg
    21841 tttacagcgg gagatgtggt tgaaagggga attagcggct caactggcta atcagggcag
    21901 ggacgacttt gtagatatcg cccaccagac cgtagtcggc atagttgaag attggcgcat
    21961 ttttatcttt attgatggcg acaatcactt ttacgccgtt gccgccaacc atatgttgga
    22021 tctgcccgga gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac
    22081 cgatataacg ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga
    22141 cttcagcatt cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca
    22201 gaccacgacc gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt
    22261 tcgcacggcg ttcgcgacag agaatttcat gacgcggagc aacataaggt accgtttctg
    22321 tcgggcactg atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa
    22381 tgatcgccag cgggctgttg atcttttcct gagcgaacgc taacccgcca tacatccggt
    22441 gttcggcgca aatgtgacca tcgacaatat ccaccgccgt ggcatcgttc accagcgccg
    22501 cattcagttg cacacttaac cgtgctgcca gagctttacc acgtttcgtc gcggccaaca
    22561 gcaacatagc ggggtgttta tctttcagca gggcagcaat gctttcggcg taattttcag
    22621 tgcgttgcag cgcgtcgttt tgctcaagaa cataaataca ttttggacca taaggcataa
    22681 ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac ctgttggccc cattgctgag
    22741 cgccgccaaa cagttcagca taacgttcag gattatcgct aaagacccag acgctgttta
    22801 attgactcat cgtttcttct ccgtaggctt cagttcaggg ctttcttcag atgttccgcc
    22861 agctcggcaa tggcctccgg cgaatcgtta tcgagaatga tgtgcttacg ttctgtttgc
    22921 ggcggtacgc gaatgccgac cagttcagca agtggcgcgc tctggctcca gtcaatatca
    22981 cttgcctgcc actgatttac cggtttttta cccgcgccga gaatggcttt catcgaagga
    23041 atgcgtggca cgttaatatc ggaggtgacg cagagcacgg ctggaacaga gagttcaata
    23101 acttcaacat catcttcaag cgtgcgttca atcaccagtg tattgccctg acgctgaata
    23161 gcactcactg cattaatcac cggaagttgc agaatttctc cgaccagcaa gccaacctgc
    23221 tgggcataaa ggtcgccgga accttcacca aagatcagta aatcgaagcc gatcttttca
    23281 attgctgccg ccagcgcttt tgcggtatcg agaggcagtg catgttcaag ttgcgcatcc
    23341 tgcaccaaat acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc
    23401 tgcaacaatg agccgccaac ggtcagcgcg gctatctcat cgtcatctgt tgcgagctgg
    23461 cttgcagctt caatggcatt gagatcgaac tggctgattt tggcgtcggc attgtcgaaa
    23521 ttcagggtgt attctggagt gacaacaatg tcctgttctt caggcaccag cttaaagcag
    23581 gttattattt tcatgatttc tcctgtgagt cgcatacgga accatctccg atggaaggta
    23641 aaaatggcct gaaaatagtg tgcgatagcg gtgcgggggg gggtgacgga gcaagctgtt
    23701 ttccttgcca acaatcttgc aaaaaaaatg cgattgctaa aacaaaagtg caatttgcgg
    23761 caatatagtt attccatgac cgtacaatct tgcattcagt gtaaataatc ttgtttagct
    23821 ctcgctaaac gcggttgtat aaccgcaata cacgccgtat tttatccggc atatattgtt
    23881 tttgcttgct catggaatag ggaggttgtc atgtatcaac gctgttttga taatgccagt
    23941 gaaacgccac ggctttcacg ttttgcattt agcgatgatc caaaatggga gtctggacat
    24001 cacgttcatg acaatgaaac cgagct
//
LOCUS       DAJTOW010000058        23365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTOW010000058 DAJTOW010000000
VERSION     DAJTOW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.058"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            126..995
                     /locus_tag="OO244_002412"
     CDS             126..995
                     /locus_tag="OO244_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5664202.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="OO244_002413"
     CDS             complement(1198..1551)
                     /locus_tag="OO244_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5664203.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO244_002414"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO244_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5664204.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="OO244_002415"
     CDS             complement(3379..3672)
                     /locus_tag="OO244_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5664205.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="OO244_002416"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="OO244_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5664206.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO244_002417"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO244_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5664207.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="OO244_002418"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="OO244_002418"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5664208.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="OO244_002419"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="OO244_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5664209.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="OO244_002420"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="OO244_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5664210.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="OO244_002421"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="OO244_002421"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5664211.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDGLNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="OO244_002422"
     CDS             11052..11627
                     /locus_tag="OO244_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5664212.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="OO244_002423"
     tRNA            11734..11809
                     /locus_tag="OO244_002423"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
     gene            12007..13272
                     /locus_tag="OO244_002424"
     CDS             12007..13272
                     /locus_tag="OO244_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5664213.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLILEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEDKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIENAMWTIPAEREPLPGVKYSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLVFTGAHDPHKPISENTVNKALRVMGYDT
                     TLDICGHGFRTMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLNANRERFISPFEYAKINNPLKQ"
     gene            complement(13594..15093)
                     /locus_tag="OO244_002425"
     CDS             complement(13594..15093)
                     /locus_tag="OO244_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5664214.1"
                     /translation="MNIRKLFYTDNISRILLFLFFPGMCVITTAACGYTEKNATGNVL
                     LLFLLLILAHRNTLTSITALLFLFCCALYAPVGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGQRNAMALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSRHGADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPEPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDADHTVFLKKGSFASRKTDDMLLLQETERALADTSTPKVIFL
                     HMMGSHPNPCDRLHSWQNNYLEQYSRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARNFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERYVQEDSLKPQPLILPDHR"
     gene            complement(15245..15985)
                     /locus_tag="OO244_002426"
     CDS             complement(15245..15985)
                     /locus_tag="OO244_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HCR5664215.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSFGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            16271..16968
                     /locus_tag="OO244_002427"
                     /pseudo
     CDS             16271..16968
                     /locus_tag="OO244_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(17075..17419)
                     /locus_tag="OO244_002428"
                     /pseudo
     CDS             complement(17075..17419)
                     /locus_tag="OO244_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
     gene            complement(17424..17705)
                     /locus_tag="OO244_002429"
                     /pseudo
     CDS             complement(17424..17705)
                     /locus_tag="OO244_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(17836..18186)
                     /locus_tag="OO244_002430"
     CDS             complement(17836..18186)
                     /locus_tag="OO244_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5664216.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(18389..19402)
                     /gene="intI1"
                     /locus_tag="OO244_002431"
     CDS             complement(18389..19402)
                     /gene="intI1"
                     /locus_tag="OO244_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5664217.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            19559..20032
                     /locus_tag="OO244_002432"
     CDS             19559..20032
                     /locus_tag="OO244_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA15"
                     /protein_id="HCR5664218.1"
                     /translation="MKLSLMAAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSSDENVLVFPSIDEALNHLKTITDHVIVSGGGEI
                     YKSLIDKVDTLHISTIDIEPEGDVYFPEIPSSFRPVFSQDFVSNINYSYQIWQKG"
     gene            20233..20580
                     /locus_tag="OO244_002433"
     CDS             20233..20580
                     /locus_tag="OO244_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCR5664219.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            20574..21413
                     /gene="sul1"
                     /locus_tag="OO244_002434"
     CDS             20574..21413
                     /gene="sul1"
                     /locus_tag="OO244_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCR5664220.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            21541..22041
                     /locus_tag="OO244_002435"
     CDS             21541..22041
                     /locus_tag="OO244_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5664221.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            22362..22430
                     /locus_tag="OO244_002436"
                     /pseudo
     CDS             22362..22430
                     /locus_tag="OO244_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            22548..23312
                     /locus_tag="OO244_002437"
     CDS             22548..23312
                     /locus_tag="OO244_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HCR5664222.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT         5582 a         6152 c         5986 g         5645 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatgg gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcagaacgcc acaatttaag cgttctgcct
    11881 acaaatcaat tctaccgaac tcaaccagat tccccccaca tcaaccccat tgtgtgggta
    11941 taattgcggg tataccccag ttcgacagaa tttgtaccct ctttaacgca ttcagaaggc
    12001 tgtactatgg cactgactga cgcaaaaatc cgggctgcaa agcccactga caaggcttat
    12061 aaactcactg acggggctgg catgtttctg ctggttcatc ctaatggttc ccgttactgg
    12121 cgtctccgtt atcgtattct gggtaaggag aagactctgg cacttggtgt gtatccagaa
    12181 gtttctctct ccgaagctcg tacaaaacgg gatgaggccc gaaaactgat tttggagggg
    12241 attgaccctt gcgaacagaa aagagctaaa aaagtagtcc ctgatttaca gctctctttt
    12301 gaacatattg cacgacgctg gcatgccagt aataaacaat gggcgcaatc acacagcgat
    12361 aaagtactca aaagcctcga gacacacgtt ttccccttta tcggcaacag ggatatcacc
    12421 acactcaata ccccggacct gcttatccct gttcgtgctg cagaagataa acagatttat
    12481 gaaatcgcca gtcgtctgca gcaaaggata tctgctgtaa tgcgttatgc cgtacagtct
    12541 ggcatcatca gatataatcc tgctctggat atggctggcg cattgaccac tgtaaaacgc
    12601 cagcatcgcc ccgctcttga tctttcacgt cttcctgaac ttttgtcgcg tattgacagt
    12661 tataaggggc agcctgtcac ccggcttgcc gttatgctga atctgctggt gtttattcgt
    12721 tccagtgaac tcagatatgc ccgctggtca gaaattgata ttgaaaatgc catgtggacc
    12781 attcctgccg aacgcgagcc tctgccaggt gtgaaatact cacaccgggg ttccaagatg
    12841 cgaacaccac atcttgtgcc actcagcaag caggctgtag ccatactgac agaacttcag
    12901 acatgggctg gtgaaaatgg tctggtcttt acgggagcac atgacccgca taaaccaatc
    12961 agtgaaaaca ccgtaaataa agccctcagg gtgatggggt atgacacaac cctggatatc
    13021 tgtgggcatg gattcaggac gatggcatgt agcgcgttaa tagaatcagg attgtggtcc
    13081 cgtgatgcag tggaacgtca gatgagtcat caggagcgta atggtgtacg tgctgcttac
    13141 attcataaag cagaacatct ggaagaacgc cgactgatgc tacagtggtg ggccgatttt
    13201 ctgaatgcaa acagagaaag atttatcagt ccatttgaat atgcaaagat taataatccg
    13261 ttaaaacagt aatcatcccg ggcaaatgcc cgggaactac tccaggacta ttttctttgt
    13321 taaaaaaaga caaacggtat gaactgatgt atttactgtt tacctgattg caccctacag
    13381 gggattatca gaatccacgg ttattcatta tttatatatc ttcaataaaa aggaacctca
    13441 aaaaaatccc gcacccacag ggtgatgcgg gataactgac gtaaggaatg ctgccgggat
    13501 ttggtggtcc cttgtgctga tatgaataca ggttctattc tatacagaat ccttttcagt
    13561 acaagagact gtattacagt ttgccatata tgattacctg tggtccggaa gaataagtgg
    13621 ctgcggtttc agactgtctt cctgtacata cctctctccg ttaaaaacca taacgggttc
    13681 attatcttcg tctgtcagcg gattgaatgg cggtatattt tcggtacgaa taccggtcag
    13741 ccactgaaaa atgcctgcga aattacgggc actgattttc ctgctgacgg actgatgaga
    13801 cgtgatgtca ctggcggtaa taatcagggg aacgctgtag cccccctgca catgaccatc
    13861 atgatgaaca ggattagcac tgtcgctgac cgacagccca tggtcagaaa agtaaagcat
    13921 tgcaaagtga cgggaatgcc ggcgaaggat accatcaagc tgaccaagaa agttatccag
    13981 tttactgatg ctggcaagat aacaggcaat ttttcgggaa tactgctcca ggtaattatt
    14041 ctgccaggag tgaagccgat cacacgggtt cggatgagac cccatcatgt gcaggaaaat
    14101 caccttcgga gtggatgtat ctgccagcgc acgttctgtt tcctgtaaca acaacatgtc
    14161 atccgtttta cgggaagcaa agctgccttt cttgaggaaa acagtatggt ccgcatcaga
    14221 agcaataaca gagatgcgtg tgtcatgctc tcccagtttt ccctgattgg atatccacca
    14281 tgtgctgtat cctgcttttg ctgccagcgc caccacgttg ttgccggaat cagggttctg
    14341 ctcatagtca taaatcagtg tccggctcag ggaaggcacg gtgctggctg ctgccgatgt
    14401 ataaccgtca ataaataaac cgggagccgt attcagccat ggtgtggttg gctcgggata
    14461 accatatacc gacatataat ccctgcggac actctcacca gtaacaataa caatcgtgtc
    14521 atataatggt gctcccggca ggattttcca gctgtcagca ccgtgccgac tcagttgttc
    14581 ataacgctgc atttcacgca atgtgtcagt tgtccccaca actgttcctt taaccatccg
    14641 taacggccag ctgtttacag agcataatac gaataacaac agagccatcg cgttacgttg
    14701 accacggtgg tgtgtccgcc agaaaataac catgaacacc agaatcgcgg cactgaccag
    14761 aaaatgataa acaggaatca tcccggtaaa ctccgcagcc tcatccgccg tggtctgcaa
    14821 caacgcgaca ataaaactgt tgttgatttt tccgtacgtc ataccgacag gtgcatacag
    14881 tgcacaacag aacagaaata acagagctgt aatggatgta agggtatttc tgtgtgcaag
    14941 aatcagaagg agaaacagca gcaacacatt cccggtggca ttcttctcag tgtatccgca
    15001 tgcggcagtg gttattacac acatgcccgg gaagaataaa aacaataaaa tccgggatat
    15061 attgtccgta taaaacagct ttctgatatt catcggagta tatcgacaac attattgtga
    15121 agagaataaa aatcagaagt ctcctgtgaa acagataaca gacaaccctg cagtataatt
    15181 actgcaggga gtcttcgttt aattacagaa gatgggtaat tattatcttt caggagcagt
    15241 tttgtcagaa gttataagtc acaccaagca tgatgtcatg acttttaaca tcaacttctg
    15301 atttatattt atcgccccac tcgtccttgt aactcacact gctgtcacct gcatcaagat
    15361 agcgatagct gaggtccaga gcgatatccg gggttacgtc atagcggaca cccgcgccaa
    15421 agctccatgc gaagttgtca gcagagcctg aacgtgacaa cgattcgcga ccactgcttc
    15481 cgtacccata atcccaggta ctgataccgg ttgttttctg gtgaattctg gcgtagccaa
    15541 tccctgcaga tacccatggt gtgaatgcac tgtcattccg gaagtcatag tacgcattca
    15601 gcatcagtgt gttgactgac acctcattct tcaggtcatc acgccagtaa ccgcctgacc
    15661 agctgtcttt atctacgtta tacttcgaat cagcttttcc acgagcgtaa aactccagtt
    15721 ccgtacgaac cggaatactg aactgcgggt aaaaatcata accggccgcg ataccgccac
    15781 tgaataccgt atcatggcca tcaccgcctt tatattttga tgtttcctct ccatcacccg
    15841 acaggaaacg ctggtctgca agtgacataa cagaagcacc tgctttaccg gtcacataaa
    15901 agcctgtttt gctctcatca gccagagcct gggtcgaaaa catgcctgcc atggcaagcg
    15961 ctgaaaccgc aataacctta ttcatttcaa tcatctccgc tatcactttc cccgaacgat
    16021 aaatggggtt gctcattcta aaaccatgca aaagataggg taaaaggaat gttttcgatc
    16081 gaatcgatcg ataaaatcga tcgtgcccga taaagttttc ttatcgtacc cataaaaatg
    16141 gtaagggctg gtttaagagg taatcgcagc ggcatggaag tattaagacc aaaacagcac
    16201 gagataaagc ggctgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
    16261 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
    16321 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa
    16381 aacatggcaa ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat
    16441 tgatatggcc atgaatggcg ttggatgccg ggcaaccgcc cgcattatgg gcgttggcct
    16501 caacacgatt ttccgccatt taaaaaactc aggccgcagt cggtaacctc gcgcatacag
    16561 ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggata cgtcggggct
    16621 aaatcgcgcc agcgctggct gttttacgcg tatgacaggc tccggaagac ggttgttgcg
    16681 cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc
    16741 tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga
    16801 aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg
    16861 aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg
    16921 catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt
    16981 accccccgga cgagtgggaa tccatggtag ggatttttac cgaaatggaa gaacactatt
    17041 agggggcacc tcagaaaacg gaaaataaag cacgctaagg catagctgac cttgccaggc
    17101 ctgcttcgcc ctgtagtgac gcgatcaacg ggcaggaaac attccccttt cgtgcatggc
    17161 aggcgcacac gagttcagac agcacggttt ccatgcgcgc caagtcggcc atcttctcgc
    17221 gcacgtcctt gagcttgtgt tcggccaggc tgctggcctc ctcgcagtgg gtgccatcgt
    17281 cgagccgcaa cagctcggca atctcgtcca gactgaaccc cagccgctgt gccgatttca
    17341 cgaatttcac ccgaaccacg tccgcctccc catagcggcg gatgctgccg taaggcttgt
    17401 ccggttcccg caacaggccc ggtcaaatgc tccttaagga actcgatgcg tacgccgcgc
    17461 tgggtgaggc cctgcaccag gcggcgcagg tcgtcgaggt tgcgcgccag acgatccatg
    17521 ctgtgcacca cgaccgtgtc gccttcgcgc acgaaggcga gcagccgttc cagttcgggc
    17581 cgccgtgtgt ccttgcccga cgccttgtcg gtgaacactt tatccacctg gatctgttcg
    17641 agctgccgtt ctgggttctg gtcgaagctg ctgacgcgga cgtaaccgat gcgctgaccg
    17701 tgcaaggtat cctcctgagg gaaatgtgtc aggaagaaat ctatgaccct tgacggcgta
    17761 tgtcaatcaa ttcggaaggc aactctattc tgacgattta gcgccggatg gtctgacgcc
    17821 aagttagggt atgcctcaac ctgacggcgg cgaatcacaa gcgtccggtt tgacgctggt
    17881 ttcggtcgca gtgctggccc gcgcctggaa gcgctgatag cactccagcc cgcagaaatg
    17941 ctccacgtac tcggcccctt ccggcgtgaa ggcggcatcg agcgggattt ccttgcagca
    18001 cacgcagcaa ctggtgctcg gttcattggc gttcatggtg gtgttcctcc attggttgac
    18061 gaagccgacg aaggccgccg ccggcatcgg cctggcgaac aggaaaccct gcaccgtgtc
    18121 gcaacccgcc tgccgcaacc acgcaaggca gtcgggtgtt tccacaccct cggctaccac
    18181 ctccattgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    18241 agcggagggg ttggatccat caggcaacga cgggctgctg ccggccatca gcggacgcag
    18301 ggaggacttt ccgcaaccgg ccgttcgatg cggcaccgat ggccttcgcg caggggtagt
    18361 gaatccgcca ggattgactt gcgctgccct acctctcact agtgaggggc ggcagcgcat
    18421 caagcggtga gcgcactccg gcaccgccaa ctttcagcac atgcgtgtaa atcatcgtcg
    18481 tagagacgtc ggaatggccg agcagatcct gcacggttcg aatgtcgtaa ccgctgcgga
    18541 gcaaggccgt cgcgaacgag tggcggaggg tgtgcggtgt ggcgggcttc gtgatgcctg
    18601 cttgttctac ggcacgtttg aaggcgcgct gaaaggtctg gtcatacatg tgatggcgac
    18661 gcacgacacc gctccgtgga tcggtcgaat gcgtgtgctg cgcaaaaacc cagaaccacg
    18721 gccaggaatg cccggcgcgc ggatacttcc gctcaagggc gtcgggaagc gcaacgccgc
    18781 tgcggccctc ggcctggtcc ttcagccacc atgcccgtgc acgcgacagc tgctcgcgca
    18841 ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc ttgccctccc
    18901 gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc aaaccctcac
    18961 tgatccgcat gcccgttcca tacagaagct gggcgaacaa acgatgctcg ccttccagaa
    19021 aaccgaggat gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc
    19081 gaggtcttcc gatctcctga agccagggca gatccgtgca cagcaccttg ccgtagaaga
    19141 acagcaaggc cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca
    19201 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt
    19261 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aaactgtaat
    19321 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
    19381 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag tctatgcctc
    19441 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
    19501 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccctaag gaagtatcgt
    19561 gaaactatca ctaatggcag caatttcgaa gaatggagtt atcggaaatg gcccagatat
    19621 tccatggagt gccaaagggg aacaattact cttcaaagcg attacctata atcagtggct
    19681 tttggtaggc cgaaagactt tcgagtcaat gggggcttta cccaaccgaa aatatgccgt
    19741 tgtaactcgt tcaagcttca cttccagtga tgagaatgta ttggtatttc catctatcga
    19801 tgaagcgcta aatcatctga agacgataac ggatcatgtg attgtgtctg gtggtggtga
    19861 aatatacaaa agcctgatcg ataaagttga tactttacat atttcaacaa tcgacattga
    19921 gccagaaggt gatgtctatt ttccagaaat ccccagtagt tttaggccag tttttagcca
    19981 agacttcgtg tctaacataa attatagtta ccaaatctgg caaaagggtt aacaagtggc
    20041 agcaactgac cgccaaaagt gtcacttgtt ttgccaaaaa gccggcaaaa caagcgccaa
    20101 ttttgtcggc agctgtgcca ggcgttagat gcactaagca cataattgct cacagccaaa
    20161 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
    20221 gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc
    20281 gcaacatccg cattaaaatc tagcgagggc tttactaagc ttgccccttc cgccgttgtc
    20341 ataatcggtt atggcatcgc attttatttt ctttctctgg ttctgaaatc catccctgtc
    20401 ggtgttgctt atgcagtctg gtcgggactc ggcgtcgtca taattacagc cattgcctgg
    20461 ttgcttcatg ggcaaaagct tgatgcgtgg ggctttgtag gtatggggct cataattgct
    20521 gcctttttgc tcgcccgatc cccatcgtgg aagtcgctgc ggaggccgac gccatggtga
    20581 cggtgttcgg cattctgaat ctcaccgagg actccttctt cgatgagagc cggcggctag
    20641 accccgccgg cgctgtcacc gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg
    20701 atgtcggacc ggccgccagc catccggacg cgaggcctgt atcgccggcc gatgagatca
    20761 gacgtattgc gccgctctta gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca
    20821 gcttccaacc ggaaacccag cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata
    20881 tccaaggatt tcctgaccct gcgctctatc ccgatattgc tgaggcggac tgcaggctgg
    20941 tggttatgca ctcagcgcag cgggatggca tcgccacccg caccggtcac cttcgacccg
    21001 aagacgcgct cgacgagatt gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga
    21061 gcggggtcgc tgccgaccgg ctcatcctcg atccggggat gggatttttc ttgagccccg
    21121 caccggaaac atcgctgcac gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc
    21181 ttccgctatt ggtctcggtg tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg
    21241 taaaggatct gggtccagcg agccttgcgg cggaacttca cgcgatcggc aatggcgctg
    21301 actacgtccg cacccacgcg cctggagatc tgcgaagcgc aatcaccttc tcggaaaccc
    21361 tcgcgaaatt tcgcagtcgc gacgccagag accgagggtt agatcatgcc tagcattcac
    21421 cttccggccg cccgctagcg gaccctggtc aggttccgcg aaggtgggcg cagacatgct
    21481 gggctcgtca ggatcaaact gcactatgag gcggcggttc ataccgcgcc aggggagcga
    21541 atggacagcg aggagcctcc gaacgttcgg gtcgcctgct cgggtgatat cgacgaggtt
    21601 gtgcggctga tgcacgacgc tgcggcgtgg atgtccgcca agggaacgcc cgcctgggac
    21661 gtcgcgcgga tcgaccggac attcgcggag accttcgtcc tgagatccga gctcctagtc
    21721 gcgagttgca gcgacggcat cgtcggctgt tgcaccttgt cggccgagga tcccgagttc
    21781 tggcccgacg ccctcaaggg ggaggccgca tatctgcaca agctcgcggt gcgacggaca
    21841 catgcgggcc ggggtgtcag ctccgcgctg atcgaggctt gccgccatgc cgcgcgaacg
    21901 caggggtgcg ccaagctgcg gctcgactgc cacccgaacc tgcgtggcct atacgagcgg
    21961 ctcggattca cccacgtcga cactttcaat cccggctggg atccaacctt catcgcagaa
    22021 cgcctagaac tcgaaatcta acgtccgttc gggcatcgag gtccatgtcg gggtgggacg
    22081 ggcccgtggc ttcaagatca cttgcagtcc gaccgcgatg tcttggttgc gcgagaggtt
    22141 gtcgatatcc tccacttcca tcatcaaccc tggataatgc cgccgccgtc atcgccgccg
    22201 acgcccgtgc cgggcttttc gggcctgtca ggcttgctcg gccttcagcc tgcctgggcg
    22261 agatctccgg cggacggatt aacggcggag cttcgccgcc tttcgtgcgt gtgaaggccg
    22321 aagatagttc tctcaaaaac atccgtttat gagagatacc aaatgtcatt ttcagaagac
    22381 gactgcacca gttgattggg cgtaatggct gttgtgcagc cagctcctga cagttcaata
    22441 tcagaagtga tctgcaccaa tctcgactat gctcaatact cgtgtgggct ctgttgcaaa
    22501 aatcgtgaag cttgagcatg cttggcggag attggacgga cggaacgatg acggatttca
    22561 agtggcgcca tttccagggt gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc
    22621 cgatcagcta tcgcgacctt gaggaaatgc tggcggaacg cggcatttcg gtcgaccata
    22681 cgacgatcta tcgctgggtc cagtgctacg ccccggagat ggagaagcgg ctgcgctggt
    22741 tctggcggcg tggctttgat ccgagctggc gcctggatga aacctacgtc aaggtgcggg
    22801 gcaagtggac ctacctgtac cgggcagtcg acaagcgggg cgacacgatc gatttctacc
    22861 tgtcgccgac ccgcagcgcc aaggcagcga agcggttcct gggcaaggcc ctgcgaggcc
    22921 tgaagcactg ggaaaagcct gccacgctca ataccgacaa agcgccgagc tatggtgcag
    22981 cgatcaccga attgaagcgc gaaggaaagc tggaccggga gacggcccac cggcaggtga
    23041 agtatctcaa taacgtgatc gaggccgatc acggaaagct caagatactg atcaagccgg
    23101 tgcgcggttt caaatcgatc cccacggcct atgccacgat caagggattc gaagtcatgc
    23161 gagccctgcg caaaggacag gctcgcccct ggtgcctgca gcccggcatc aggggcgagg
    23221 tgcgccttgt ggagagagct tttggcattg ggccctcggc gctgacggag gccatgggca
    23281 tgctcaacca ccatttcgca gcagccgcct gatcggcgca gagcgacagc ctacctctga
    23341 ctgccgccaa tctttgcaac agagc
//
LOCUS       DAJTOW010000059        22953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTOW010000059 DAJTOW010000000
VERSION     DAJTOW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.059"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="OO244_002438"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="OO244_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5664223.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1210..1872
                     /locus_tag="OO244_002439"
     CDS             1210..1872
                     /locus_tag="OO244_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5664224.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="OO244_002440"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="OO244_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5664225.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2760
                     /locus_tag="OO244_002441"
                     /pseudo
     CDS             2406..2760
                     /locus_tag="OO244_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2798..3103
                     /locus_tag="OO244_002442"
     CDS             2798..3103
                     /locus_tag="OO244_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5664226.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3106..3510
                     /locus_tag="OO244_002443"
     CDS             3106..3510
                     /locus_tag="OO244_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5664227.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3500..3799
                     /locus_tag="OO244_002444"
     CDS             3500..3799
                     /locus_tag="OO244_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5664228.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4281
                     /locus_tag="OO244_002445"
     CDS             3985..4281
                     /locus_tag="OO244_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5664229.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4351..5337
                     /gene="yqjG"
                     /locus_tag="OO244_002446"
     CDS             4351..5337
                     /gene="yqjG"
                     /locus_tag="OO244_002446"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5664230.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5630..5998
                     /locus_tag="OO244_002447"
     CDS             5630..5998
                     /locus_tag="OO244_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5664231.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="OO244_002448"
                     /pseudo
     CDS             complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="OO244_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            7206..7907
                     /locus_tag="OO244_002449"
     CDS             7206..7907
                     /locus_tag="OO244_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5664232.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7930..8094
                     /locus_tag="OO244_002450"
     CDS             7930..8094
                     /locus_tag="OO244_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664233.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8306..9616)
                     /locus_tag="OO244_002451"
     CDS             complement(8306..9616)
                     /locus_tag="OO244_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5664234.1"
                     /translation="MFDSTLHPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9644..10975)
                     /locus_tag="OO244_002452"
     CDS             complement(9644..10975)
                     /locus_tag="OO244_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5664235.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="OO244_002453"
     CDS             complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="OO244_002453"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5664236.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12686..13075)
                     /locus_tag="OO244_002454"
     CDS             complement(12686..13075)
                     /locus_tag="OO244_002454"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5664237.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="OO244_002455"
     CDS             complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="OO244_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5664238.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDFLSAIKYARVKPIRDENGLAVGFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="OO244_002456"
     CDS             complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="OO244_002456"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5664239.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="OO244_002457"
     CDS             complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="OO244_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5664240.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="OO244_002458"
     CDS             complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="OO244_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5664241.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLSDGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="OO244_002459"
     CDS             complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="OO244_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5664242.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20346..20564
                     /gene="tdcR"
                     /locus_tag="OO244_002460"
     CDS             20346..20564
                     /gene="tdcR"
                     /locus_tag="OO244_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5664243.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            20820..21360
                     /locus_tag="OO244_002461"
                     /pseudo
     CDS             20820..21360
                     /locus_tag="OO244_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21382..21729
                     /locus_tag="OO244_002462"
     CDS             21382..21729
                     /locus_tag="OO244_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664244.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21898..22548
                     /locus_tag="OO244_002463"
     CDS             21898..22548
                     /locus_tag="OO244_002463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664245.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6194 a         5787 c         5289 g         5683 t
ORIGIN      
        1 caatcatcaa atttatatat gaccaatggc atacccatga ataaagtggt ataataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgct gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa
      181 actgcctgcc gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gtcatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      421 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccagcgtag
      601 ccataacccg tactggaaaa aactacacga acacattgat tcccgtaccg ttgataactg
      661 gtgtgatgac cacgatcaaa ttctcaaggc gttaattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcatc tggaaaacac caaaatcatg ttatttaacg aaaccagcga
      781 cgatttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      841 tacggccacc agcggcagca aatgaaattc ctgtccgaca ggcgctgcaa tgcgcctgtt
      901 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 cccctgcaat cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgctttt gaatgctttc ttatctcacg
     1081 ttttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaatg gcttgcttcc ggcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggttgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcatt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gttcttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa
     2761 ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga
     2821 acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc
     2881 tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa
     2941 acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa cccgtgtcgc
     3001 ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg gaattggcgc
     3061 tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat
     3121 cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg
     3181 gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagct
     3241 aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt
     3301 atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct gaatgcgatg
     3361 attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg gacgttacgt
     3421 aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa cgatcggcaa
     3481 ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact
     3541 gcttagccag atccagcaac aacggctgga tctttccgcc agtcgtcgtg aatggctgga
     3601 ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct cctgggcgct
     3661 ggttggcagt agagtgatgg cgatctggac gattcgccat cctaatatgc tggtccgctg
     3721 ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga ccctcaagca
     3781 gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta tccatcaaat
     3841 tttttgaata tttttgacag ttttccttgc taacaatcat cattcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatagc gcgcccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
     4021 cacattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgct gacggcattt
     4141 ttatttcaca gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca
     4201 atttctggcg gattcctgct gctggcaatt accggtccgg gcgcgtatag catcggccgc
     4261 ctgctgaata aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc
     4321 tcctcgtttt tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat
     4381 gacacctggt acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt
     4441 cgtaactggc tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca
     4501 gagaaagatc gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg
     4561 atcatgcgca aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg
     4621 atgctggaaa acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc
     4681 tatcaacatg aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga
     4741 gttactgttc ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca
     4801 gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac
     4861 tacccaccag ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt
     4921 aacaacggcg tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg
     4981 gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg
     5041 accggcaacc agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat
     5101 ccagtgtatg tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg
     5161 tatggcttcc tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat
     5221 cacatccgta atcattactt ccgcagccat aagaccatca accctacggg gattatttca
     5281 attggtccgt ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa
     5341 aaggcacctt acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc
     5401 ggcgtgaacg ccttatcaaa cctacaaagc gtttatattc aatatgttac aatgaccgcg
     5461 taggccagat aagcgtagcg catctggcga ttttgcgctt gccatcagtc ttagcgcgcc
     5521 ttcacggcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     5581 cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta
     5641 tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     5701 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     5761 aggctggcaa cgcgctggtg gcgaaggcat cctgacgata atctacggta ttctggtgtt
     5821 tttaccgtgg tgggcggttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     5881 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     5941 ggcgggtacg ccaggcgaaa accgctttgg accagatcca aaactggaac aagaataatg
     6001 attaagcacc ttgtaaaagg tgcttcaaat tgttgacaaa gtgcgctttg tttatgccgg
     6061 atgcggcgta aacgccttat ccagcctaca aattgccaaa attcaatata ttgcagtgac
     6121 tgcgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcagc agtcagaagc
     6181 accttgtgaa aggtgcttaa tagattattt tccagcgaac aactttggaa tttcccgcag
     6241 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
     6301 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
     6361 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctaacgtt tatcttcaat
     6421 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
     6481 acgagcggta tccgctaccg caattccacg atatttcaca cgcgtcactt cagataacgg
     6541 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
     6601 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat
     6661 atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg tgatgattgc
     6721 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     6781 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gagcttccgc
     6841 atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     6901 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     6961 ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc
     7021 tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     7081 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     7141 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     7201 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     7261 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     7321 ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct
     7381 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     7441 aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc cagccgggtg
     7501 tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg cagctttggc
     7561 tggatacctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt aatatggaca
     7621 agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta cgccaacaag
     7681 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     7741 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     7801 aagcggcgct aacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     7861 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     7921 gagatgatga tgagtaaaaa atcgaccaaa aagcgccagc cagcgaagcc cgtggtggcg
     7981 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     8041 gctatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     8101 gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa acacaacaac atcaacaaat
     8161 taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     8221 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     8281 cggcacaata ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     8341 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     8401 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     8461 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     8521 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag
     8581 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     8641 ttgcggcaca aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     8701 gagaaagcat cagcgcacgc gccagtcgtt catcatccgc tccgaagtgt tctgctacaa
     8761 ccaccacagg cattattgca gtgatcccct ggttacccga gccggagtta ctcatagccg
     8821 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     8881 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     8941 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     9001 cgagaataaa gcggatcgcc gcaaatggga cttcattgac gaacttcagg atctcagcca
     9061 gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc gccctctgcc acacacgcct
     9121 ggtgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     9181 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     9241 cattgcaagg ttcctggatc ttaacagaga ctttccccgc cgccagcagt gctttggcat
     9301 cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     9361 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     9421 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     9481 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     9541 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggatgta
     9601 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     9661 acagagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     9721 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     9781 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     9841 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     9901 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     9961 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
    10021 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
    10081 aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
    10141 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
    10201 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
    10261 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
    10321 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
    10381 tcgacacgcc cagcgcagcg actaccagca gcttggtcag caccatgtcg gtcgaaattt
    10441 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
    10501 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
    10561 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
    10621 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
    10681 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
    10741 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
    10801 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
    10861 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
    10921 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
    10981 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
    11041 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
    11101 aatcttcaca atcaagactt taaaataacg gagatggata agaattttct actaaattaa
    11161 tcacaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
    11221 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
    11281 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
    11341 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
    11401 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
    11461 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
    11521 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgctgccat tgaacaggcc
    11581 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
    11641 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
    11701 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
    11761 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
    11821 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga
    11881 cgacgcagcg ctacggcacg acacggcaca ttgagcggac caggcagcac gccttcagtg
    11941 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
    12001 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
    12061 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
    12121 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
    12181 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
    12241 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
    12301 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
    12361 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
    12421 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca
    12481 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
    12541 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
    12601 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
    12661 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
    12721 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
    12781 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
    12841 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
    12901 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
    12961 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
    13021 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
    13081 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
    13141 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
    13201 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
    13261 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
    13321 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
    13381 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
    13441 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
    13501 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
    13561 atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
    13621 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
    13681 tattcaccgt cgatttcaaa gcccaccgcc aggccgtttt cgtcacggat tggtttcacg
    13741 cgggcatatt tgatggcaga caggaagtcc gtcgccaccg acaggcccgc gatgccgcat
    13801 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
    13861 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
    13921 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
    13981 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
    14041 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
    14101 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
    14161 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
    14221 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
    14281 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
    14341 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
    14401 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
    14461 tcctgtgcct gctgctcatt gaggacgcca gctttaaagt cgcgctcaat gtagatatcc
    14521 aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
    14581 agataagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg gcgagagata
    14641 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
    14701 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
    14761 agatcggcaa attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg
    14821 cggcgatagt caccgataat gcgcccacga ccatagccat ctggtaaacc ggtcagcacg
    14881 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
    14941 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
    15001 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
    15061 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
    15121 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
    15181 atacggatgc cttccattac cttttcccac aattccgtgg tggcaggcgt cgcttcggcg
    15241 agaaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
    15301 atttcgcttt tccagtccgt acctttaaag ccaagccatg cgtcagcgta cagcttatcg
    15361 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
    15421 gcaaattctg cgggcgcgtt aactttacct aaatgaatgg catccaaagc aatcattttt
    15481 tcttcgttag tcggaataac gacgcaaatg acatgcgcat tttcactgga aacaattcgc
    15541 tcgccaaaag agttagaacg attattcatt tctgtatcaa tctctacgcc taatacagcc
    15601 aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat
    15661 ataattccat ccagccgacg taatgaagct gcatgtccgg caatatgacg ggcaattcgg
    15721 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    15781 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    15841 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    15901 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    15961 gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc
    16021 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    16081 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    16141 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    16201 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    16261 tttgcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    16321 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    16381 aaggccacgc tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    16441 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    16501 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacaatc gctggcatcg
    16561 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    16621 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    16681 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    16741 agacggcgct ttacggatag catacatcgg caacaggcac agcagggatg cgataatcgg
    16801 tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    16861 ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca ctttggtttt
    16921 gtcaccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    16981 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    17041 tgtaatcgcg aacgttgttt tcgtaccggt catggacgca aagtggttag ccagataaga
    17101 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gagtaaacag
    17161 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    17221 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    17281 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    17341 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    17401 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    17461 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    17521 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    17581 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    17641 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    17701 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    17761 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    17821 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    17881 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    17941 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    18001 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    18061 ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa
    18121 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    18181 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    18241 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg acagggtgcc
    18301 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    18361 ttctgactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    18421 accagcaatt aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc
    18481 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    18541 tgggataaaa atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    18601 gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    18661 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    18721 cgaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc
    18781 atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    18841 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg
    18901 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc
    18961 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    19021 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    19081 aaaacaaaca actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa
    19141 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    19201 cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    19261 ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    19321 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    19381 tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc
    19441 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    19501 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    19561 tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    19621 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    19681 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    19741 accaccgctt cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    19801 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    19861 ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg
    19921 gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact
    19981 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    20041 gttaattacc tcattgacgg catgaagtgt atcaaaatga aataaacagg atatgtgcga
    20101 ccactcacaa attaactttc attactttcc aaggtatcgt tattacaatt aaacatcttt
    20161 aaatatcaac aagttaaagc ataaaaatca gcataaaccc tgatttttat caaaaaaaaa
    20221 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    20281 aggggctgag accatatttc aagcaattac cggataatta ctaggcaaaa tttgagttaa
    20341 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    20401 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    20461 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    20521 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    20581 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg gtcgccgcga
    20641 taaggtcaga cgaagtcaac aagggggatt ttcactgtgc tttacatcag cgtaggacac
    20701 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgtaag
    20761 tgtcgtgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    20821 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    20881 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaattg
    20941 tctccaccaa tgacgttaat aaaatttaca gtataagtaa tgaactccgg agagtattat
    21001 ctgcaattac tgcattgaat ttctatcaag gcgatgttcc ttctgtcatg attccgaatc
    21061 caaccggaaa atatgagtcc attcatcata gatatttcta caggagaaca tgatgattat
    21121 atcatacaaa cattagatgt aggcactttt gcaccttttg gtgaacaatg tacttgctca
    21181 gccgtcaata aaaaagagct ggaatgtatt aaggagacga tttctaagta ttgcgcaaag
    21241 ttcacccaaa aagaagccat cttaacaccc cctaggcact ttaataaaac ctgcattact
    21301 tcagattgtt ggcaaattct ctttttttca cccgatcatt ttaataatga tttttattga
    21361 ttatttataa aggataaaat aatgttccct gtttcatcaa ttggtaatga tattagtagt
    21421 gatttagtcc gtcgaaaaat gaatgacctt cctgaaagcc ccataggaaa taacctcgaa
    21481 gcactagcac caggtataga aaaactaaaa cagacctcta ttcaaatggt cactttactt
    21541 aacgcgttac aaccaagtgg aaaatgcatt atcactggtg attttcaaaa agaattagcg
    21601 tacttacaaa atgtaattct ttataatgac tcgtctcttc gtctggattt tttaggttat
    21661 aacacccaaa ttattcaacg atcggacaat acctgcgaac ttaccattaa tgaaccgtta
    21721 aaaaactagg aaatatctac aggcaatatc aatgttaatt gcccattaaa agatatttac
    21781 aatgaaatca ggaggttgaa cgtaatcttt agttgtggga ctggagatat agttgatcta
    21841 tcctctctgg acttacgtaa tgtcgattta gattattatg atttcacaga taaacatatg
    21901 gctaatacta ttctaaatca ttttaaattg gattcaacaa attttactaa tgccaacatg
    21961 tttcaggtta attttgttag ttcaacacaa aacgccacaa tctcctggga ttatttacta
    22021 aaaataacgc ctgttttaat aagcattagc gatatgtatt ctgaagaaaa aatcaagttt
    22081 gtcgaaagtt gtttaaatga gcttggagac attaccgaag aacaattaaa aattatgaga
    22141 tttgcaatta taaaatctat accaagggca actcttacag ataaattaga aaatgaatta
    22201 acaaaagaaa tatataaaag ctcatcgaaa atcaacaatt gcttgaacag aattaaatta
    22261 ccagagatga aagaattctc atcaaaaaaa acatatgatt acatcgatac aatcattgaa
    22321 gattatgaaa atatcaaaga aaatgcttat ctggtcgtcc cccaaattaa ttatactatg
    22381 gatttaaaca tagaagactc tagctcagaa gagttaatac cctcgagaaa gacgaaaatt
    22441 ctccggacaa tggctttgag gtcggggaat ataacacata tgaagcatat aactcagaga
    22501 atcaatattt taccagagag gactatacgt ataattacga ccttttaaat gcaatataga
    22561 cttaataact tcttacaaag tctattatta ataccgtaga tattcatctt accctgtgca
    22621 ccaaaagcaa aaacttatcg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga
    22681 tgcgctacgc ttatcaggcc tacgccatct ctgcaatata ttgaatttgc gtgcttttgt
    22741 aggcaggata aggcgttcac gcagcatccg gcatgaataa agcgcactca acaatctgaa
    22801 acccgccgga acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt
    22861 tgggtatagt gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc
    22921 gaagcgggtt tttacgtata tcaggtgaaa ctg
//
LOCUS       DAJTOW010000060        22415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTOW010000060 DAJTOW010000000
VERSION     DAJTOW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.060"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO244_002464"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO244_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5664246.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="OO244_002465"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="OO244_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5664247.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="OO244_002466"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="OO244_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5664248.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO244_002467"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO244_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5664249.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="OO244_002468"
     CDS             3251..5011
                     /locus_tag="OO244_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5664250.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="OO244_002469"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="OO244_002469"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5664251.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO244_002470"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO244_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5664252.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO244_002471"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO244_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5664253.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO244_002472"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO244_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5664254.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO244_002473"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO244_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5664255.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="OO244_002474"
     CDS             complement(9679..11586)
                     /locus_tag="OO244_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5664256.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO244_002475"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO244_002475"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5664257.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="OO244_002476"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="OO244_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5664258.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO244_002477"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO244_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5664259.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO244_002478"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO244_002478"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5664260.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17630)
                     /locus_tag="OO244_002479"
     CDS             complement(16893..17630)
                     /locus_tag="OO244_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5664261.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            complement(17596..18111)
                     /locus_tag="OO244_002480"
     CDS             complement(17596..18111)
                     /locus_tag="OO244_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5664262.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18119..18661)
                     /locus_tag="OO244_002481"
     CDS             complement(18119..18661)
                     /locus_tag="OO244_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5664263.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18759..19742)
                     /locus_tag="OO244_002482"
     CDS             complement(18759..19742)
                     /locus_tag="OO244_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5664264.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19733..22333)
                     /locus_tag="OO244_002483"
     CDS             complement(19733..22333)
                     /locus_tag="OO244_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5664265.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
BASE COUNT         5558 a         5875 c         5261 g         5721 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gcggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agctaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgtgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgccccaa tctggtggtt tccggcgcgt cagcctggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaac acaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccggcc
    17401 caacgcgttg ggcgattgat tttgctgtta ataagccaag attcctgact ggtattagtc
    17461 agtaaaatag atatcgattc tctgtctgcc ggaaagataa atcgcgttcc gccgaccacc
    17521 actcctgcct gcaattcacc cgccaccagc agcgataaaa cggcgaaaat caagagcgct
    17581 aatctgttcc acgtattagt cataatgaag tacaaatgtc gcgctggcat tcactttacc
    17641 cgactgcaca tcgctattga caggtcgcaa ttgcgcataa aaagttaatg gcaccttacc
    17701 gctatgtagg ctgtagtctt tactcgcctg gttaagcgga atcagtatct tcgcatcatc
    17761 cagaatcgca attgccagcc cactggctgc atccgtacca ctttcaatgg acagcatttc
    17821 gctctgcact ccgtcggggg ttcctgaaaa ggtgacatca accgtgctga catctgttcc
    17881 gcaatcctgc aaattaatga tgaaagattt ctccgggcca taattcccat gaaaaaaacg
    17941 tgtcagcgag acatcgccta actgcacatt gatttgcttg ctatcctgtg ccagcacgca
    18001 tgtattacgg cgaaacgatc cgctaatgtt gaccgtgcca tcatgggcaa atagctcaca
    18061 gccccccgtt aatcccagta agaacagaat ccagattatc cgtttcaaca tcactccttc
    18121 actggtaaac cagatcaaaa tagagaaccg ccgtcgcatt accgcccgtt gctcccgcct
    18181 ttgtggactt ataacgaagt tgatatttga gtgtgttatc cccggctttt aagggcgtaa
    18241 gaggctggac ctgattgagc gggatttctt gctgactttg cgcatcaaga atttgcacgg
    18301 caatccccgt tgccatacca ccgcttccgc ctgtgtcagt tagtttgagc agtgtcggcg
    18361 caagggtgtc agcctcgccg ctaaaaagga cgtccgcgcc agtaatacca gcggcacaac
    18421 cggtgatatc aatattcaaa tcagcagcag tgcttacggt attcgcggcc tgaaaatcac
    18481 tgatattaaa atcaccaata tgaatatttt gtaatgcact tttggtggcg acatcacagc
    18541 cctgatcgac cacattccca taaatatgca tactgacttg ttcaggccca gccatcccga
    18601 catggatgtt aagaaggcta aagaacaaaa taacgcactg atatatcgtt tttttcttca
    18661 tacgttcacg cgttaatcaa aatttattct caccgttgcc gtggcttcaa atcgcccggt
    18721 ttctggtggt acgcctgtcg tgctgactgg atagacattc attcgcggat actgtctgca
    18781 tcatcaggaa tagagagcgg tagtgtccca ccagcagggg aaataatgtt gtgctggcta
    18841 tcggtcacca ccacgcccac gccaggacgt gacgtcttaa tcgcctgggg atagctgggg
    18901 tctgaggttg ccgtcagact cagattgacg ttaactaacc ctgcgtttcc cgtgttacac
    18961 ctgaccggaa ttgcaagttc tgccgttctg gccccaagag gcttatgccc tacaacacga
    19021 aagttagccg caaatacgtc gcccagatca acctcgattg tttctccggc attgacggaa
    19081 catgagccaa gtgaattgat cgtaccagac aggtaataca ctaaaaccgg tgtaccagta
    19141 gtcgtgcagg aatcacctgc gggtatgttg tagcattcaa ataatcgcgc cagcgccata
    19201 ggaggaatga cgaccgaacc aacgaacggc tgcaatattt tcagggaaaa gctaccgcta
    19261 ttaccgctag ccccctggcc acctaaacgt tgaccgccgg gatcgcaaat atgccatcgt
    19321 gtatctgccc ctgtgcccac gttttcgaaa ggaaccgcag taggagaact gcctttaacc
    19381 tgcaataaaa ctttggcggc aagatagtca ttaattttgt accagttgtc gcctaaatag
    19441 actaacggtg aatccgcggc gaatgcccat cggccacttc tgacattgtc tttatcgcaa
    19501 tcacaggaaa cataataatc attcgattcg ttccactgat aaaatttgtc tttaactact
    19561 ccagccgtat tttcttcttc atccagagtt ttaacgccaa aattgacgcc aatatacgga
    19621 ttcccctgag tggtatgaca ctgcccgata taagcagcag tttgagcgca ccatagcatt
    19681 aagacggcgg caaaaaatag tcgtagtaat aacacgcatt tttctccaaa gattatctgc
    19741 atatggcatc cattcgcgta atcgcagagt taatttgttg ttcggtaagc tggtaactga
    19801 tatgacaaat ttgatcggga gcttcgcccc agcgtacagt tatcgctccc tgagcgggca
    19861 aaccggacat atagagcgag ccatcatcat caataatgcc gctattagtc tgtatgccgt
    19921 ccagcgtcgc tatcgcgcca aaacgcagcg gtataccctg ctttgatgtt ttcattaata
    19981 cccttgctcc cgcatgggca ttgaactccg ccagaaccaa cgcccctttg gtgggaacca
    20041 cgttgactac tgcattttca agatccacgt tacgttttaa cgatgccgca tcaagtgcga
    20101 ttcgattttc ccgataagaa gtggcatagg ggacaacggt atatccgcgc cagtcagttt
    20161 tcagcccggt gctgttcgcc acacgagtat tctctgcgcc tggtgctgca atcagcacgt
    20221 tagtttcccc cagcgattgc cccagggtaa tgccctgtga atgggcaact aaactgcctg
    20281 aaagagcgta gttcagttgt tgttgactgc cgttatcgct gtagttgtag cccactcggg
    20341 catctgcaaa cgcccctttg tagtccatgc tggcgctacc attagccgtt ttcccctcgc
    20401 tgttatatcc ctgttgcacg ctgtagctca ggttaccgtc atcgagtagt gtctccgtta
    20461 aacccaggtt ttgtgtaatg tgcccttcgt tatcgatact ggtgttagag gtcatccagg
    20521 cgtaattttt tttcctggta tagctatcat tacttctggg gagcaacaga ttgagcggca
    20581 gggaaaaatt tagtgcaaac acctgatcag catcaggttg accacgggac ttactgtagt
    20641 tccaggaaac ccccagcgag agatctttaa tttgcgtgtt gtagccaaac tgtaatagcc
    20701 gatcctgttg atcggtatgc cagtaagttt gctggctacc acttaaataa aacgaaccgt
    20761 actcgcctaa ttgctgggag atattgacct gcagtttgcc acgtttggtg taaaacaaat
    20821 tgtaataacg cgaccacatc ggcgtatctt catcatcacc gtcattaaat tcgtagccat
    20881 ccatatgttt atacatggtg tcggaaaggg tgtagaaacc cgacgtcgaa tagcgatagc
    20941 ccatcaattg aaaattagtg cccaattggt tgagcgtttt ggcatacaga aaacgatatg
    21001 actgaccttt atgttcgctt tggtctgcca gcgtactctt cgcctgggtc gcatcgaacg
    21061 aaattgctcc gaaatcgcca aggttaaatc ccagaccaaa catggcggca cggtaatatt
    21121 cagcatattg tccaccacca taccatgtcg ttccccacgg tccgccccac tgcaacgtgg
    21181 tctgggcaaa tttgctctcc tgctgttcat tactattggt tctgtatttc gccagtgtca
    21241 ccgcgtattt gattcgcccc tgacgctgga gtaatggcac gctggaaaac ggtacgctgt
    21301 agctattgac gctgccgtcc gcttctttga tttcaactaa caaatcaccg ctcgacgacg
    21361 tggaatagag atcactaatt tcaaaagcac caggcgatac ataagtttgg taaatggtgt
    21421 aaccattttg cttaatcgtt atctgggcat tgcttttggc gataccacgc accacaggcg
    21481 caaacccttt caggctgtct ggcagcatgt tgtcatctga cgccagctgc acaccacgaa
    21541 agctgacact atcgaaaaaa tcgcctgcag tataatcatc acctacggtc agttcgcctt
    21601 ttagtggaat aaccgcccgc tgcaaatagc tgctgagatt cttccattct gcggtttggc
    21661 cactactgcg actccatgtt gaattgttgc gtaatctcca tgggcctaaa ttaacgccac
    21721 tgtttaaatt cagaaaatag ctgtcgccag aatcactgtc tgcgctgcta tgaatactgt
    21781 tagccccgct aaacgaatat cccagtaata gcgcgttgat cccttcatcc caacgttcag
    21841 gggggacata accacgggca ttacttttga tggcgatttg cggtactgaa atttccagac
    21901 ggagtttatt cacattaaac gttacgctgg catccggaat aatctccgcc aatggcacac
    21961 acacttgttt gtccagttgt ttaaaagccg gaaacgcatc agtattaaca cccattgcat
    22021 ccaggctttc agtcgtcaaa caggcggcaa tccccttctc tggcgttatc tcaacaaaat
    22081 tgacgttgcg cgtatctaca attgtctgat tcagaacaat atcgacacga taaacccctg
    22141 gcgttatttt ttgtcctttt tcgaaacgag acaaatcggc caccattgag ggatcatctt
    22201 tgaaaaaagc aggatcgaac caggtttcag cttgtgcatt gaacgctgcc gacacaaaca
    22261 ccaccagccc accaataaac gatggcactc caccagagct taacaggctg tgttgtcgat
    22321 gagttctata catctcgctc tcttaactga agacggctta cgatttacag ttgagttgcg
    22381 gcgtttccgc cccgtagtca ttgatggttc gatac
//
LOCUS       DAJTOW010000061        22281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTOW010000061 DAJTOW010000000
VERSION     DAJTOW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.061"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..1028)
                     /locus_tag="OO244_002484"
     CDS             complement(<1..1028)
                     /locus_tag="OO244_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664266.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OO244_002485"
     CDS             complement(1130..2110)
                     /locus_tag="OO244_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5664267.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OO244_002486"
     CDS             complement(2118..2828)
                     /locus_tag="OO244_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5664268.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO244_002487"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO244_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5664269.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OO244_002488"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OO244_002488"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5664270.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OO244_002489"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OO244_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5664271.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OO244_002490"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OO244_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5664272.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OO244_002491"
     CDS             9405..9515
                     /locus_tag="OO244_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5664273.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO244_002492"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO244_002492"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5664274.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSLVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO244_002493"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO244_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5664275.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OO244_002494"
     CDS             12397..13029
                     /locus_tag="OO244_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5664276.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO244_002495"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO244_002495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5664277.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OO244_002496"
     CDS             16561..17493
                     /locus_tag="OO244_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5664278.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OO244_002497"
     CDS             complement(17534..18964)
                     /locus_tag="OO244_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5664279.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO244_002498"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO244_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5664280.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO244_002499"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO244_002499"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5664281.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OO244_002500"
     CDS             complement(20825..21094)
                     /locus_tag="OO244_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5664282.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OO244_002501"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OO244_002501"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5664283.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OO244_002502"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OO244_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5664284.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5514 a         5584 c         5333 g         5850 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcaccgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacgt ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc accagtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagacca gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJTOW010000062        21860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTOW010000062 DAJTOW010000000
VERSION     DAJTOW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.062"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..86)
                     /locus_tag="OO244_002503"
     CDS             complement(<1..86)
                     /locus_tag="OO244_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5664285.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACR"
     gene            94..594
                     /gene="tnpA"
                     /locus_tag="OO244_002504"
     CDS             94..594
                     /gene="tnpA"
                     /locus_tag="OO244_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5664286.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(633..980)
                     /gene="grxD"
                     /locus_tag="OO244_002505"
     CDS             complement(633..980)
                     /gene="grxD"
                     /locus_tag="OO244_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5664287.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1315..2142
                     /gene="mepH"
                     /locus_tag="OO244_002506"
     CDS             1315..2142
                     /gene="mepH"
                     /locus_tag="OO244_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5664288.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2270..2851
                     /gene="sodB"
                     /locus_tag="OO244_002507"
     CDS             2270..2851
                     /gene="sodB"
                     /locus_tag="OO244_002507"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5664289.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWAMVNWEFVAKNLAA"
     gene            complement(2997..4166)
                     /locus_tag="OO244_002508"
     CDS             complement(2997..4166)
                     /locus_tag="OO244_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664290.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4332..4421)
                     /gene="ynhF"
                     /locus_tag="OO244_002509"
     CDS             complement(4332..4421)
                     /gene="ynhF"
                     /locus_tag="OO244_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5664291.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4720..5745
                     /gene="purR"
                     /locus_tag="OO244_002510"
     CDS             4720..5745
                     /gene="purR"
                     /locus_tag="OO244_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5664292.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5742..6674)
                     /locus_tag="OO244_002511"
     CDS             complement(5742..6674)
                     /locus_tag="OO244_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5664293.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6787..7998
                     /locus_tag="OO244_002512"
     CDS             6787..7998
                     /locus_tag="OO244_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5664294.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8289..9437
                     /gene="cfa"
                     /locus_tag="OO244_002513"
     CDS             8289..9437
                     /gene="cfa"
                     /locus_tag="OO244_002513"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5664295.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9477..10118)
                     /gene="ribE"
                     /locus_tag="OO244_002514"
     CDS             complement(9477..10118)
                     /gene="ribE"
                     /locus_tag="OO244_002514"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5664296.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10333..11706
                     /gene="mdtK"
                     /locus_tag="OO244_002515"
     CDS             10333..11706
                     /gene="mdtK"
                     /locus_tag="OO244_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5664297.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11747..13003)
                     /locus_tag="OO244_002516"
     CDS             complement(11747..13003)
                     /locus_tag="OO244_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5664298.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGARQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13311..13387
                     /locus_tag="OO244_002517"
     tRNA            13311..13387
                     /locus_tag="OO244_002517"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13345..13347,aa:Val,seq:gac)
     gene            13392..13468
                     /locus_tag="OO244_002518"
     tRNA            13392..13468
                     /locus_tag="OO244_002518"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13426..13428,aa:Val,seq:gac)
     gene            13576..13881
                     /locus_tag="OO244_002519"
     CDS             13576..13881
                     /locus_tag="OO244_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5664299.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14007..15429
                     /locus_tag="OO244_002520"
                     /pseudo
     CDS             14007..15429
                     /locus_tag="OO244_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15441..16253)
                     /locus_tag="OO244_002521"
     CDS             complement(15441..16253)
                     /locus_tag="OO244_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664300.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16257..17042)
                     /locus_tag="OO244_002522"
     CDS             complement(16257..17042)
                     /locus_tag="OO244_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5664301.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17039..17707)
                     /locus_tag="OO244_002523"
     CDS             complement(17039..17707)
                     /locus_tag="OO244_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5664302.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17771..18409)
                     /locus_tag="OO244_002524"
     CDS             complement(17771..18409)
                     /locus_tag="OO244_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5664303.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18422..20523)
                     /locus_tag="OO244_002525"
                     /pseudo
     CDS             complement(18422..20523)
                     /locus_tag="OO244_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20544..21170)
                     /locus_tag="OO244_002526"
     CDS             complement(20544..21170)
                     /locus_tag="OO244_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5664304.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21626..21835)
                     /gene="fumD"
                     /locus_tag="OO244_002527"
     CDS             complement(21626..21835)
                     /gene="fumD"
                     /locus_tag="OO244_002527"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5664305.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5467 a         5662 c         5072 g         5659 t
ORIGIN      
        1 cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg tcttaactgg
       61 aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt gaaaaaagaa
      121 accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc
      181 accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg cacttacttt
      241 tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga accagatcac
      301 gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc
      361 aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt caggtattac
      421 tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg tgcgccaata
      481 tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc
      541 ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga ctgacggatg
      601 cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc ttcagacttg
      661 tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata catttcgatc
      721 acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa ggtcggccag
      781 ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc aacataggca
      841 aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc gcagctcggc
      901 agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg gcgttggatt
      961 ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca gtaatctgac
     1021 attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt tgctatttta
     1081 tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc gcggcaaact
     1141 ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc gccaacgaaa
     1201 cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta gaatcatcaa
     1261 gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg ctcagtggcg
     1321 cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct gcctttaacg
     1381 cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca tatcaccaaa
     1441 aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc cagccagaaa
     1501 acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc ttctgttaaa
     1561 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
     1621 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
     1681 gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc gaaggtgcaa
     1741 aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
     1801 ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
     1861 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
     1921 ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg tactcaggga
     1981 cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
     2041 cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat
     2101 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
     2161 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     2221 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     2281 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     2341 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
     2401 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     2461 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     2521 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     2581 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     2641 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     2701 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     2761 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcga tggtgaactg
     2821 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     2881 tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     2941 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     3001 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     3061 gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag cccagcggaa
     3121 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     3181 actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat ctgtaacgga
     3241 ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc gccgaactca
     3301 ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa aaagcctttc
     3361 aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc gatagagaat
     3421 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     3481 tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc cagtaccgtc
     3541 gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt tttcacttca
     3601 ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     3661 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     3721 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     3781 ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa accaaaaaat
     3841 gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata atccggcgcg
     3901 atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     3961 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac gccaactgca
     4021 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     4081 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc
     4141 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
     4201 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
     4261 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
     4321 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa accgaggacg
     4381 ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
     4441 ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
     4501 atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc caaaattcca
     4561 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
     4621 ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga
     4681 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
     4741 gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa aacacgtttc
     4801 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
     4861 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
     4921 agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa ttgcttccag
     4981 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
     5041 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
     5101 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
     5161 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac
     5221 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg
     5281 ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg
     5341 atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga atccggttat
     5401 cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
     5461 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg cgtcccgcag
     5521 gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac gccggcgctg
     5581 accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat
     5641 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg tttgattgaa
     5701 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
     5761 gtctcttccg gctcacgcag ccactcctta ttcagcgtct cactatcgct gagatactca
     5821 agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
     5881 tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag ccacggtttg
     5941 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
     6001 tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat acgtttaggt
     6061 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
     6121 aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact ccagcttagc
     6181 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatt
     6241 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
     6301 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
     6361 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
     6421 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
     6481 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggtacc
     6541 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac acgatgcagc
     6601 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
     6661 tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa tattaccgtt
     6721 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
     6781 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
     6841 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg
     6901 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
     6961 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
     7021 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc cacgctgctg
     7081 gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg gcaagcatta
     7141 gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat catgccgctg
     7201 gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt ccatttttcc
     7261 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
     7321 tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac ctttaccgac
     7381 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg ttcagccagt
     7441 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
     7501 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
     7561 cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
     7621 gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa
     7681 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc
     7741 gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt gcagaacact
     7801 ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat tagtatcagc
     7861 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt
     7921 tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc cgaagtcgct
     7981 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
     8041 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
     8101 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg
     8161 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
     8221 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
     8281 gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac aactggtacc
     8341 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
     8401 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc tctttggggt
     8461 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
     8521 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
     8581 ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaagg
     8641 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
     8701 cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
     8761 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
     8821 ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc ggcgtcacca
     8881 tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat gtcaccattt
     8941 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
     9001 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
     9061 aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
     9121 atccctggat taataaatat atttttccga acggttgcct gccctctgta cgccagattg
     9181 ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg
     9241 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
     9301 actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc gcaggtgctt
     9361 tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt gaaaacggcc
     9421 ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag ttcagatcag
     9481 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
     9541 tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc aagttttttc
     9601 ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa acgcgttggc
     9661 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata
     9721 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
     9781 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
     9841 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
     9901 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
     9961 tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc caggccgtcc
    10021 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
    10081 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
    10141 taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg aaggggtaag
    10201 aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
    10261 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
    10321 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
    10381 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
    10441 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
    10501 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
    10561 ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
    10621 tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat ccgctacatg
    10681 gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg
    10741 ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa
    10801 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc ggtgaactat
    10861 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
    10921 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
    10981 tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc ggttatgaaa
    11041 cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt gacactgttt
    11101 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
    11161 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    11221 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    11281 accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac ggtttcactg
    11341 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    11401 ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg
    11461 attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac agcttactgg
    11521 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    11581 gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc cattatgatg
    11641 atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca acgagcgtcc
    11701 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    11761 gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac agagtttgcc
    11821 gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc acattgatga
    11881 agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    11941 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    12001 tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta ctgccgtcga
    12061 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct gcgccataac
    12121 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    12181 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    12241 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc ccccccacat
    12301 tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    12361 gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga ttattcatgg
    12421 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    12481 taatctcaca attgctggct tctccacttg gatacaccaa caattcacca ccttcatcta
    12541 ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt
    12601 tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc
    12661 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    12721 gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt
    12781 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg
    12841 ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg ccagcttcaa
    12901 caatcaactc gccttcagta agttgcctcg cgccaaggac ctcgccggtc ttaacaattt
    12961 gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    13021 catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta tccacgacag
    13081 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    13141 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    13201 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    13261 acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag
    13321 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    13381 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    13441 ggtggttcga gtccacttgg acgcaccaga ttttcttaat ctggtctttt cctttttccc
    13501 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    13561 agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt
    13621 ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca ggaacctggt
    13681 tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctacctt
    13741 ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg actgaaaaat
    13801 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact tagtcaaatc
    13861 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    13921 tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc
    13981 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    14041 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    14101 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    14161 tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg tatctcgaat
    14221 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    14281 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    14341 gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    14401 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    14461 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
    14521 caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag gtcgatgcgt
    14581 gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg gcagtggcta
    14641 ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac cgcgataacg
    14701 caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc gaagccgatt
    14761 tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca ttaaatgctg
    14821 agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata gtagaggaaa
    14881 tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg aatgtcgatt
    14941 cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac tgggcagaaa
    15001 aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc tggcgttatg
    15061 tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat gaacacgacc
    15121 ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg gcaatccctt
    15181 cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat ccctgatgtt
    15241 ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc gttcggcgcg
    15301 ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg tgcagaaatt
    15361 ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg gcgtctttcg
    15421 tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt aagacgatag
    15481 cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg aactctttcg
    15541 ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga gtcgaccacg
    15601 cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt gtcgccgctg
    15661 ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc attactggcg
    15721 acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt cgcaatccga
    15781 ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac ccgtgcgcgt
    15841 tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac gctataaccc
    15901 atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc ctcacaacag
    15961 ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat ctctccgggg
    16021 tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc atccgccagc
    16081 cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc ttcctcaatc
    16141 agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag gaaccagcga
    16201 tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat cattcgttag
    16261 tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg ccccgtggtg
    16321 caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc aaaatgcgcc
    16381 tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg atagagacac
    16441 aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac ataggcgacc
    16501 tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc ttccccctgc
    16561 ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca cccctgacga
    16621 cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa gccgagccag
    16681 catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt aaccgcagtt
    16741 gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac aaagagtaac
    16801 gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt ctcaccatga
    16861 tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc cagcaccaac
    16921 cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa ttcgggagtg
    16981 aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg cgacgggttc
    17041 atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc ggttttccgg
    17101 caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga atttctgggc
    17161 tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa atgggcggaa
    17221 agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact ttagtgacag
    17281 ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag ccgatgcact
    17341 gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc gtcgggcata
    17401 cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag tgatactgcg
    17461 tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt ccctgagcgg
    17521 ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg caccgtgact
    17581 cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct aacagagaag
    17641 acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg cgtcgagtga
    17701 aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc catgacatct
    17761 ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat aagccataag
    17821 ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc gatgactcca
    17881 cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg gcttcaatct
    17941 gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa ggacgaggat
    18001 acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc tgctccacaa
    18061 cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa tagtaatcat
    18121 cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta atgctttcca
    18181 gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac acggtgcgcg
    18241 ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt tcagcaggtt
    18301 ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc cgcttcgcct
    18361 cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc ttacccattc
    18421 cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc agccccagac
    18481 gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt tctttgtaga
    18541 acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct tcagtaaaga
    18601 ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt tcattacgca
    18661 tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag atacgctctg
    18721 cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg aagaatttgg
    18781 cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg gtcattggcc
    18841 agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc gaccactttg
    18901 catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg taagcatctt
    18961 cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag ccggaaccaa
    19021 taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta acaatggaac
    19081 cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc gaccagagtt
    19141 tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg atggcatatg
    19201 aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag taaaaatctt
    19261 taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca tactcttcag
    19321 ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg cgatgcaact
    19381 gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc aggttaccaa
    19441 tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag atggtggtat
    19501 ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac tctttcaccc
    19561 gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg ctcattttta
    19621 ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta cgaaagccga
    19681 ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct tcagccgcgc
    19741 cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgaatac tctgcccacg
    19801 attgtggcgt acttggcacg acatggttgt tattcgcacc aataagttca gtcatcatgt
    19861 aatcattgag acgcttcatc tcctgacgat cggcaatgtt gacccctttc gtcccttcaa
    19921 ccgcaatcgc tttcaggttt ttcgaaccca ttattgcgcc agttcccgca ccgccgctgt
    19981 ggttacggct attcaacatg ccagagagag gaataaggtt ttccccagcc tgaccaatag
    20041 ccgccacaca ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt gcgcgcgtcc
    20101 cttttcccca taagaaatcg gctttttcca ggctaacttt gtcatcttta atcttcagcc
    20161 ataccggtga tttcgccttc ccttcgataa taatgacgtc gtatccagcg aatttcattt
    20221 gcgctgcaaa aaagccaccc atatgggcat cgcgactaaa tttcctttgg taaaagtaga
    20281 aagtgaggtg atatttacgc gagaactaca gggggcacca gatccagtta atgggccggt
    20341 agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctagcggta cttcgtcata
    20401 cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac tggaatcttc
    20461 gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc aaccgttagc
    20521 catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat aatttttaac
    20581 gcccctgttg ggcaggcgtt tgcgcattca ccgcataaca cacatttcga ggatttttta
    20641 ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc gctacagcca
    20701 atacaacgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt aatcgcacca
    20761 atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag
    20821 ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa gaaataattg
    20881 cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa gttggtacaa
    20941 gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag cacccctttg
    21001 ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca acccaaaagt
    21061 gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat acgcaagaat
    21121 tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat ttatttcgca
    21181 tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat tcaactaata
    21241 cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg tatgagaaaa
    21301 gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt gattattctg
    21361 agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat aagcgaactg
    21421 atatttatta tcatttgaaa taaatttaac ttaagcgaca atcatttgtg acgttcattg
    21481 cagaaaataa aaataacatt atcgctataa attaaaatac ataccgaaat tcgtataatt
    21541 tcagcagttg ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg
    21601 aagcttaccg cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg
    21661 cctgcttttc cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaatacgc
    21721 ctgcgataac aatatgcttt ggttcctgcc ccagatcgcg catttccagc acaactttac
    21781 cgacaactct gcacatttct cggtacagtt catcctcttt tgtccgattt cccattcttt
    21841 atacctattt atcatgccaa
//
LOCUS       DAJTOW010000063        21507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTOW010000063 DAJTOW010000000
VERSION     DAJTOW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.063"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO244_002528"
     CDS             complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO244_002528"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5664306.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO244_002529"
     CDS             complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO244_002529"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5664307.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3125..3424)
                     /locus_tag="OO244_002530"
     CDS             complement(3125..3424)
                     /locus_tag="OO244_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5664308.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3599..4594
                     /gene="wecH"
                     /locus_tag="OO244_002531"
     CDS             3599..4594
                     /gene="wecH"
                     /locus_tag="OO244_002531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5664309.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4636..5073)
                     /locus_tag="OO244_002532"
     CDS             complement(4636..5073)
                     /locus_tag="OO244_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5664310.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5119..5460)
                     /locus_tag="OO244_002533"
     CDS             complement(5119..5460)
                     /locus_tag="OO244_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664311.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO244_002534"
     CDS             complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO244_002534"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5664312.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO244_002535"
                     /pseudo
     CDS             complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO244_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8843..9835
                     /gene="xylF"
                     /locus_tag="OO244_002536"
     CDS             8843..9835
                     /gene="xylF"
                     /locus_tag="OO244_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5664313.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9913..11454
                     /gene="xylG"
                     /locus_tag="OO244_002537"
     CDS             9913..11454
                     /gene="xylG"
                     /locus_tag="OO244_002537"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5664314.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11432..12613
                     /gene="xylH"
                     /locus_tag="OO244_002538"
     CDS             11432..12613
                     /gene="xylH"
                     /locus_tag="OO244_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5664315.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRCIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12691..13221
                     /locus_tag="OO244_002539"
     CDS             12691..13221
                     /locus_tag="OO244_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5664316.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13333..13869
                     /locus_tag="OO244_002540"
     CDS             13333..13869
                     /locus_tag="OO244_002540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5664317.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13977..14801)
                     /locus_tag="OO244_002541"
     CDS             complement(13977..14801)
                     /locus_tag="OO244_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5664318.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15121..17151
                     /gene="malS"
                     /locus_tag="OO244_002542"
     CDS             15121..17151
                     /gene="malS"
                     /locus_tag="OO244_002542"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5664319.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAIINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17329..18582
                     /gene="avtA"
                     /locus_tag="OO244_002543"
     CDS             17329..18582
                     /gene="avtA"
                     /locus_tag="OO244_002543"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5664320.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18733..19206)
                     /locus_tag="OO244_002544"
     CDS             complement(18733..19206)
                     /locus_tag="OO244_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5664321.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHREMGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19308..20156)
                     /gene="yiaJ"
                     /locus_tag="OO244_002545"
     CDS             complement(19308..20156)
                     /gene="yiaJ"
                     /locus_tag="OO244_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5664322.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20356..21354
                     /gene="yiaK"
                     /locus_tag="OO244_002546"
     CDS             20356..21354
                     /gene="yiaK"
                     /locus_tag="OO244_002546"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5664323.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     DVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21366..>21507
                     /locus_tag="OO244_002547"
     CDS             21366..>21507
                     /locus_tag="OO244_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5664324.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5406 a         5489 c         5277 g         5335 t
ORIGIN      
        1 aaagcctgcc agatggcagg ctttttaata acggcgttat tactgcaaca gcgaaatatc
       61 cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg gtcagacggt tgagacgcaa
      121 ttctttgtca tcaaccatga ccatcacttt atcgaagaaa gcatcaaccg gctcacgcag
      181 ctctgccagt tcgaccagcg catcctggta acgaccttct gcaaagtacg gctccagctt
      241 gtcacgtagc accacaacct gcatcgccag tttaatttct tccggctctt tcagggtaga
      301 ggcattcacg cgatcgctca gcacttcgtc agatttcgcc aggatgttag atacacgttt
      361 gttcgccgcc gccagtgctg cagctgcttc cagggtacgg aagtgcgata ccgctttcat
      421 gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc agtaccgcct ggatggtgtc
      481 aacggtataa ccttcgtcct gataccaggc gcggaagcga ccgagcataa agtcgataac
      541 atcatcaact acgttggcat tagtcagctt atcgccatac agacgcaccg cttcttcggt
      601 cagcgtttgc agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc
      661 ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat
      721 acccgccaga gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg
      781 caggtcatca ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc
      841 cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc
      901 gaagaccatg ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt
      961 aacgtcagcg ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt
     1021 gtcacgcagc gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc
     1081 ttccagacgt tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg
     1141 aacgactttc tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac
     1201 gaagataaag ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc
     1261 acctttcatg gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt
     1321 tgcggtcaga acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct
     1381 taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc
     1441 tttacgttct tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg
     1501 atcggcgttg tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg
     1561 atcggactga atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac
     1621 ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat
     1681 cggcagtttc gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc
     1741 cttcacatgg gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg
     1801 gtcaacggta ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc
     1861 agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc
     1921 ttccgccagg ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt
     1981 gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc
     2041 caggctgcgc agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt
     2101 ctcagacata gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg
     2161 ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg
     2221 ctgacgctcg gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc
     2281 tttcagaata cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg
     2341 cgcttctttc tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa
     2401 gttgtaagtg gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc
     2461 cagcgggccg tcgctccaga ctaggtcgta aacgctgtct acgccctgaa tgtacatggc
     2521 cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac
     2581 ctgctggaag taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag
     2641 tccccaggcc ccaagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat
     2701 agtcgggtcc atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg
     2761 cgatggctta atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc
     2821 gtagcgacca tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg
     2881 ccccagcgcg cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa
     2941 tggttgaaca atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc
     3001 ctggaaggtc ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta
     3061 aaggaaggga tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca
     3121 gggcttaacg catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact
     3181 gctctttacg gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg
     3241 tgacgtcgat tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg
     3301 cttccatatt caacgaacgc agaggatcaa cccttacccg ttgtgctttc tgcacgggtg
     3361 acggtgtaga acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca
     3421 tcattaaatt accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg
     3481 atatcttacg aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca
     3541 gtgcgccaac caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat
     3601 gcagcccaaa atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat
     3661 gattcacacc actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga
     3721 tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc
     3781 cggctatctc ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt
     3841 atgtctgatt ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa
     3901 tatggagtta gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt
     3961 cttcgcgatt gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg
     4021 aaaaatgttg ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc
     4081 gcagaaaatt gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt
     4141 ttactacatt ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa
     4201 agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg gtttttatta tctctcgcgg
     4261 gacattatat gaattgcagt ggcgcggaaa ttttgccgat acctggtatc tttactgtgg
     4321 gccgatggtt tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac
     4381 gcgtaccatt cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca
     4441 cgccttgatt atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact
     4501 ggatattatt tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt
     4561 acaacgaatc gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga
     4621 ccgggcttac gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg
     4681 gtttattccg gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa
     4741 gaaagccagt ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac
     4801 catcagtaga gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt
     4861 ggtcggtatg ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag
     4921 tcctaacacc agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca
     4981 tagccctaac aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa
     5041 ggccggtgaa taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt
     5101 gtaagagctt tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac
     5161 aaaaaaggcg atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc
     5221 gataaacaag agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt
     5281 atccagttgc ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat
     5341 taacacgcca agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac
     5401 cagataaacc agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat
     5461 aacgatctcc atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa
     5521 aatgccatta acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag
     5581 atatatagat gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa
     5641 tggcagaagt tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata
     5701 acgctgcgca tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag
     5761 cgatttctct ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac
     5821 atcccccccc gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca
     5881 gtactcacta cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc
     5941 atgcacgaca tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc
     6001 cagttcattg gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt
     6061 attgtgtggt gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc
     6121 atcagcctgt tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc
     6181 ggcccaatcc agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg
     6241 tagcgcatgg caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac
     6301 agcaaaatag acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc
     6361 cacaccaact gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat
     6421 accccacgct ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa
     6481 tgcgggcatc tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg
     6541 ctttgcgaca tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc
     6601 cgtcatacgc agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat
     6661 ctccggctca tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag
     6721 gttgccggta atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc
     6781 acagcgcccg tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa
     6841 ggttgctcca tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg
     6901 atcgcccaga gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc
     6961 cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc
     7021 accctgctcg ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata
     7081 catatcgatc gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac
     7141 tgcacagtta gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc
     7201 tggcgaccac tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct
     7261 gccagtgaca tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa
     7321 cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc
     7381 gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta
     7441 ctttgacgac gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg
     7501 agaatttcat acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc
     7561 cagcccagtt gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg
     7621 gcggtggcta tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg
     7681 ttcagtttaa tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca
     7741 tcgtaatcat attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc
     7801 tagaagccga ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca
     7861 cgctcctgac gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg
     7921 acatagtttt caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc
     7981 gcccagctga agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt
     8041 gtaaagcagt tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc
     8101 aggacatcaa ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg
     8161 gaaacatcca catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat
     8221 gcgacatctg ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga
     8281 ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag
     8341 gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag
     8401 tgacggaatg ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg
     8461 tcaaaatagg cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg
     8521 tcgtaatatt gggcactccc tttcaattgc tcaattatgt tatttcacac tgctattgag
     8581 ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc
     8641 ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa
     8701 tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt
     8761 ttagttgcta aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc
     8821 taactacaga aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc
     8881 acttctgctt accaacgttg cggcacacgc caaagaagtc aaaataggta tggcgattga
     8941 tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc
     9001 tctcggtgcg aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca
     9061 gattgaaaac atgattaacc ggggtgtcga tgttcttgtc attattccgt ataacggtca
     9121 ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt attaaagtat tagcttacga
     9181 ccgtatgatt aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg
     9241 tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa ggtaattact tcctgatggg
     9301 cggctcgccg gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa
     9361 accttacgtt gattctggaa aaattaaagt cgttggtgac caatgggttg atggctggtt
     9421 accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga
     9481 tgctgtagtt gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca
     9541 aggtttatca gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg
     9601 tattgctgcc ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac
     9661 tgccgcagaa attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact
     9721 gaataatggc ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa
     9781 caacatcaaa gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt
     9841 acgccccggc gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta
     9901 ggtggagtcg ttatgcctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt
     9961 gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt
    10021 ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat
    10081 ggctcctacg aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc
    10141 gataccgaac gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg
    10201 accgtgctgg aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat
    10261 gacctgatga cgctacgcta tcagaagctg ctcgcacagg tcagtttatc catttcacct
    10321 gatacccgcg ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca
    10381 cttaataaac aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag
    10441 gaaacgtcgg ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt
    10501 tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc
    10561 gacggacagc acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc
    10621 atgatggtcg agcgagagtt aaccgcgatt taccctaatg aaccacatac caccggagat
    10681 gaaatattac gtattgaaca tctgacggca tggcatccgg tcaatcgtca tattaaacga
    10741 gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt
    10801 ggtgccggac gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa
    10861 ggaaaaattt atattgatgg caaacaggta gatattcgta actgtcagca agccatcgcc
    10921 caggggattg cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg
    10981 gttggtaaaa atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt
    11041 gatgacgcgg cagagcaaaa atgtattctg gaatcaattc agcaactcaa agttaaaacg
    11101 tcgtcccccg accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc
    11161 gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg atgaacctac caggggtatt
    11221 gatattggcg cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt
    11281 gccgttattg tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg
    11341 gtgatgcatg aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag
    11401 gtgatggaag ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa
    11461 attggccgta ccgacatccg gtggcttttc tgggctgaaa tcactgaatt tgcaggtctt
    11521 cgtgatgatt gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc
    11581 ctacttaagc gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct
    11641 cgcggtagga atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat
    11701 gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc
    11761 acttaccatc attgtgacgt tggttctggg actgcttctc ggtgcctgga acggatggtg
    11821 ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg
    11881 cggcatactc attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc
    11941 acaaattggg caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt
    12001 aatggctttt gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca
    12061 gtctccggcc tctaccgcag tagtcggtcg ccaagcttta accgctatca tcgtattagg
    12121 cgcaatctgg ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt
    12181 gctgttactc ggcggaatgt ttatggcaac gcggacggca tttggacgat gcatttatgc
    12241 catcggcggc aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact
    12301 tgccgtgttc gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg
    12361 acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg
    12421 cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg
    12481 ggcatttatc atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg
    12541 gcagtatatc gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa
    12601 acgccgttct tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga
    12661 gtttttgcat gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta
    12721 ctgttcaatg ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta
    12781 caggcatcac aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat
    12841 aaaatcaagg actggttagg agatgtcgtc attgccgact tcgacgacaa acagatcgag
    12901 caagcgctgg ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca
    12961 gaaagttacc cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca
    13021 tttttgcatt taaaagagaa aggcgttaac cgctttgctt tttatggtct tccggaatca
    13081 agcggcaaac gttgggccac tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa
    13141 aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg
    13201 caaaatcggc tggcagactg actacaaacg ctgccaccgc aaaccgggat tattgccgtt
    13261 actgacgccc gagcgcggca tattctgcaa gtatgtgaac atctacatat tcccgtaccg
    13321 gaaaaattat gcgtgattgg catcgataac gaagaactga cccgctatct gtcgcgtgtc
    13381 gccctttctt cggtcgctca gggcgcgcgg caaatgggct atcaggcggc aaaactgttg
    13441 catcgattat tagataaaga agaaatgccg ctacagcgaa ttttggtccc accagttcgc
    13501 gtcattgaac ggcgctcaac agattatcgc tcgctgaccg atcccgccgt tattcaggcc
    13561 atgcattaca ttcgtaatca cgcctgtaaa gggattaaag tggatcaggt actggatgcg
    13621 gtcgggatct cgcgctccaa tcttgagaag cgttttaaag aagaggtggg tgaaaccatc
    13681 catgcaatga ttcatgccga gaagctggag aaagcgcgca gtctgctgat ttcaaccacc
    13741 ttgtcgatca atgagatatc gcaaatgtgc ggttatccat cgctgcaata tttctactct
    13801 gtttttaaaa aagcatatga cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc
    13861 atgttgtagc gatgatgaga attgtcggcg tcacatcagg gaactcagca cagccacctg
    13921 atgcgagcaa aagccttatc cgaccgacaa atcagataag gctcgaggaa atgaaattac
    13981 atatgcgccg cgattaaccg ttggttatcc tggtacattg cgaacaggta gttgttataa
    14041 ctcttccctt tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc
    14101 tgatccgctt tacgcagctg tgcacgcgat ttacggaacg aagagtaagc cgggtgcgta
    14161 ttcaggttag tgacataggc gctcaccgat tctttgacag aactaaactg tgagtaccct
    14221 ttcactttac ccggcgcatt ggtacaacgt cctttcatgc atttcatgcc gaacagattg
    14281 ttattgttgc gcgccagctt cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc
    14341 accatactgg tagggataat gtctacgcgt tcaagcaagg tattccacgg gatttttcgc
    14401 gtattaccag accacttcac cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca
    14461 gcaggtgccc attggccctg atactgtttt gaaatcagcc agttacgttc tgcagtaatg
    14521 gctgcatttt ggctggtaat gtaaggcatt acggtccgga gaaacgcctt tttccttggt
    14581 gttccggaag ggtattttcg caaatcagga agtgaactac tctttgcact attgcgagaa
    14641 tactcttgtt tactgcttac ctgtttatta ctagctttag ttaagtggga cttttgactc
    14701 gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt gagtaacata
    14761 agaatcatcg ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt
    14821 taatcattcc aacaccttat atttttcaca aatttgagag ttgaatctca aatcatatca
    14881 aaaatagctg tcaagagcac cccaaggaat agtccaaatc tgaaactatg tcacgagtta
    14941 acgattcaga ttggcgctaa atcgcagaaa atgtgggggt tatcgcaaaa ttcagccgtt
    15001 ttttgcgcgc gatcgctcac ccttgcttct catcctgtgg acttaccgct cagggatgag
    15061 ttttgtctgg cttatcgctg gcaaactgtc tgaaatcgca gcaataagga ctcatccacc
    15121 atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg
    15181 acttctccgg ggttccctgc ctttagcgaa cagggaacgg gaacatttgt cagccacgcg
    15241 cagttgccca aaggtacgcg tccactcacg ctaaattttg accaacagtg ctggcagcct
    15301 gcagatgcga taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct
    15361 caatggcgat tgttcaggga cggcaaatat acgctgcaaa tagacacccg ctccggtacg
    15421 ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa
    15481 tgcccgaaat gggatggatt accgctcacg ctggatgtca gcgccacttt cccggaagga
    15541 gccgccgtac gggattatta cagccaacaa attgcgatag tgaagaacgg tcaaataacg
    15601 ttacaacccg ctgctaccag caacggttta ctcctgctgg aacgggcaga aactgacaca
    15661 tccgcccctt tcgactggca taacgccacg gtttactttg tgctgacaga tcgtttcgaa
    15721 aacggcgatc ccagtaatga ccagagttac ggacgtcata aagacggtat ggcggaaatt
    15781 ggcacttttc acggcggcga tttacgcggc ctgaccaaca aactggatta cctccagcag
    15841 ttgggcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg ctgggtcggc
    15901 ggtggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg
    15961 acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca
    16021 catcagcgcg gtattcgtat tctctttgat gtcgtgatga accacaccag ctatgccacg
    16081 ctggcggata tgcaggagta tcagtttggc gcgttatatc tttctggtga cgaagtgaaa
    16141 aaaacgctgg gtgaacgctg gagcgactgg aaacctgccg ccggacaaac ctggcatagc
    16201 tttaacgatt acattaattt cagcgacaaa acaggctggg ataaatggtg gggaaaaaac
    16261 tggatcagaa cggatatcgg cgattacgac aatcctggat tcgacgatct cactatgtcg
    16321 ctggcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc
    16381 tataaaaaca aaacggatac tcacgctaaa gtcatcgaag gctttacacc tcgcgattac
    16441 ttaacccact ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat
    16501 accgccaaac atgttgagtt gcccgcctgg cagcaactga aaaccgaagc cagctccgcg
    16561 cttcgcgaat ggaaaaaaac taaccccgac aaagcattag atgacaaacc tttctggatg
    16621 accggtgaag cctggggcca cggcgtgatg caaagtgact actatcgcca cggcttcgat
    16681 gcgataatca atttcgatta tcaggagcag gcggcgaaag cagtcgattg tctggcgcag
    16741 atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac
    16801 ctctcgtcgc atgatacccg tctgttccgt gaagggggcg acaaagcagc agagttatta
    16861 ctattagcgc caggcgcggt acaaatcttt tatggcgatg aatcctcgcg tccgttcggt
    16921 cctacaggtt ctgatccgct gcaaggtaca cgttcggata tgaactggca ggatgttagc
    16981 ggtaaatctg ccgccaacgt cgcgcactgg cagaaaatca gccagttccg cgcccgccat
    17041 cccgcaattg gcgcgggcaa acaaacgaca ctttcgctga agcagggcta cggctttgtt
    17101 cgtgagcatg gcgacgataa agtgctggtc atctgggctg ggcaacagtg acttttccgg
    17161 cttcccgttc gtcagtacct cgggaagccg ccaaccagga taaaatgtca gccctaatca
    17221 gcgttgcggg ataaagcacc gctcactctt caacagaccg atttgcaccc cagcaaatgt
    17281 agcgttattg ttaccttcct tgctacagag ttcgacagat atcccgctat gacattctcc
    17341 ctttttggtg acaaatttac ccgccactcc ggcattacgc tgctgatgga agatctgaac
    17401 gacggtttac gcacgcctgg cgcgattatg ctcggcggcg gtaatccggc gcagatcccg
    17461 gaaatgcagg actacttcca gacgctactg accgacatgc tggaaagtgg caaagcgact
    17521 gatgcactgt gtaactacga cggtccacag gggaaaacgg agctactcac actgcttgcc
    17581 ggaatgctgc gcgagaagtt gggttgggat atcgaaccac agaatattgc actaacaaac
    17641 ggcagccaga gcgcgttttt ctacttattt aacctgtttg ccggacgccg tgccgatggt
    17701 cgggtcaaaa aagtgctgtt cccgctggca ccggaataca ttggctacgc tgacgccgga
    17761 ctggaagaag atctgtttgt ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag
    17821 tttaaatacc acgtcgattt tgagcatctg catattggcg aagaaaccgg gatgatttgc
    17881 gtctcccggc cgacgaatcc aacaggtaat gtgattactg acgaagagtt gctgaagctt
    17941 gacgcgctgg cgaatcaaca cggcattccg ctggtgattg ataacgctta tggcgtcccg
    18001 ttcccgggta tcatcttcag tgaagggcgc ccgctatgga atccgaatat cgtactgtgc
    18061 atgagtcttt ccaagctggg gctgcctggc tcccgctgcg gcattatcat cgccaatgaa
    18121 aaaatcatca ctgccatcac caatatgaac ggcattatca gcctggcacc tggcggtatt
    18181 ggtccggcga tgatgtgtga aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca
    18241 gtcatcaaac cgttttacta ccagcgtgtt caggaaacta tcgccatcat tcgccgctat
    18301 ttaccggaaa atcgctgcct gattcataaa ccggaaggag ccattttcct ctggctatgg
    18361 tttaaggatt tgcccattac gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg
    18421 ctgatggtgc cggggcacaa cttcttcccg ggactggata aaccgtggcc gcatacgcat
    18481 caatgtatgc gcatgaacta cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt
    18541 ctggcggaag agatagaaag agcctgggct gaaagtcact aaaaattgcc tgatgcgcta
    18601 cgcttatcag gcctacatga tttctgcaat atattgaatt tggaagaatt tgtaggccgg
    18661 ataaggcgtt tacgccgcat ctggcataaa caaagcgcac tattccggcg ttgagaaacg
    18721 ccggaaaacg tttcaaacag gctgctgctg tagccgatgc cgctgcactt tctcgacatc
    18781 catgcactgc aacgcctttg tcgggcaggc ttcaacacag gccggcccca tctcccgatg
    18841 ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc
    18901 accaaacggg caagccagca tacagctttt acagccaatg caacgtgttt gttcaacgaa
    18961 aatatgccca tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc acggtgcatc
    19021 ttcacactga tgacaggcta ctggcgtggt ccagctgtgg tctttaatga cacgaatacg
    19081 ggaaataaac tcgtctggtg acaacgcagc gcaatcctga ttctcctgat gcgacactgc
    19141 gcaagccact tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa taataaaccg
    19201 gttcatgatc attcctttgc actaaaacag cagagtgcca gagagcgcgt ccctactgct
    19261 ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg
    19321 cccaggtcat ccctgacagt aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc
    19381 agtggtttca ggagattttt ctctcccacc tgtttcagac gtgatgtcga aagcgaaatc
    19441 gacacggcgt acggcacccg cccatgaata tcaaacaccg gaacagcaat acaggagacg
    19501 ccgagttcgt tttcttctct gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg
    19561 tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta acggctggat ctcatgctga
    19621 tggttttccc agtatgactt cacgtagtcc gggtgaccaa acgccatgta gatcttgccc
    19681 attgcggaac agtagagcgg catatgctgg ccaatatagg cacgggttcg cagcatcccg
    19741 gttgtgggtt ccagcttata aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta
    19801 atggtttcac cagtggcgat gttcagtgcc tcaagatgcg gagcggcgat atgaatgata
    19861 ttcagcgaag acagcgcctt ctgcccgacg gcaataaatt tggtagtcag gcggtaactc
    19921 cctgcggcgg gcgcggtggt cacatagccg caggactgta atccctgcaa taagcgatgg
    19981 acggtactct tatttaaacc agccagctcc gaaagatgcg ccaacggaca accgtttgga
    20041 tagttgctca aaatctcaat cagcatcaac ccgcgaaaca gactctggct tccggctgga
    20101 cgctcttttt cctgcgccat ctcgttctct ttttttccca tcacttcttt ccccattttg
    20161 tcgcgtcctg atggtagcgc aaagtgtgcc gtagttcacg atctcgacag ataatttata
    20221 accacttgat ttttatgtct tttgaaattc atcaatcaga ttgctttgtt aaaaagtgat
    20281 cgatatattt gaaatctagt tccgcatatt gaaattttaa gccaaaaaag cgatcaaaaa
    20341 acaaggaagc ctaggatgaa agtgacattt gagcagttaa aagcagcctt taatcgggtc
    20401 ttaatttcac gcggcgttga cagcgaaacg gctgacgcct gtgcagagat gttcgcccgc
    20461 accaccgaat ccggcgttta ttctcacgac gttaatcgtt tccctcgttt cattcaacaa
    20521 ctggaaaacg gcgatatcat tcctgatgcc caacccaaac gtataaccag cctcggcgca
    20581 attgaacagt gggatgccca gcgttcgatc ggcaacctga cggcgaaaaa gatgatggat
    20641 cgcgccattg aactggctgc cgatcacggt attggtctgg tggcacttcg taatgccaac
    20701 cactggatgc gcggcggcag ctacggctgg caggcggcgg aaaaaggcta tattggcatt
    20761 tgctggacca actccatcgc cgtaatgccg ccgtggggcg caaaagagtg tcgcatcggc
    20821 actaacccgc tgatcgtcgc cattccttcc acgccgatca ccatggtcga tatgtcgatg
    20881 tcgatgttct cttacggcat gttagaagtt aaccgcctgg caggccgtca gctcccggtc
    20941 gatggtggct ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaagaat
    21001 cgccgcattt tgccgatggg ctactggaaa ggttctggca tgtcgattgt gctggatatg
    21061 atcgctactc tcctttccga cggcgcatcg gttgccgaag tcacccagga caacagcgac
    21121 gaatacggca tttcacaaat ttttattgcc attgaagtgg acaagcttat cgacggtccc
    21181 acccgcgatg ccaagctgca acgtatcatg gattacgtta ctaccgctga acgcgctgac
    21241 gaaaaccagg ccatccgctt acccggccac gaatttacta ccctgctggc cgaaaaccgc
    21301 cgtaacggca tcactgttga tgacagcgtg tgggccaaaa tccaggcgtt atgaggagat
    21361 aagtcatgat ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc gccgccattg
    21421 aaaaggcgct cgattttctg cgcgctacca acttcaacgt cctggagccg ggcgttgtcg
    21481 aaatcgacgg caagaatatt tatgcgc
//
LOCUS       DAJTOW010000064        21469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTOW010000064 DAJTOW010000000
VERSION     DAJTOW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.064"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO244_002548"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO244_002548"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5664325.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO244_002549"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO244_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5664326.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO244_002550"
     CDS             complement(2649..3200)
                     /locus_tag="OO244_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5664327.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO244_002551"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO244_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5664328.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO244_002552"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO244_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO244_002553"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO244_002553"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5664329.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO244_002554"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO244_002554"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5664330.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO244_002555"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO244_002555"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5664331.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO244_002556"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO244_002556"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5664332.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO244_002557"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO244_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5664333.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO244_002558"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO244_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5664334.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO244_002559"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO244_002559"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5664335.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO244_002560"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO244_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5664336.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO244_002561"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO244_002561"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5664337.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO244_002562"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO244_002562"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5664338.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO244_002563"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO244_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5664339.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO244_002564"
     CDS             complement(18170..18565)
                     /locus_tag="OO244_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5664340.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO244_002565"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO244_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5664341.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO244_002566"
     CDS             19481..20074
                     /locus_tag="OO244_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5664342.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO244_002567"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO244_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5664343.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21469
                     /locus_tag="OO244_002568"
     CDS             20794..>21469
                     /locus_tag="OO244_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5664344.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKG"
BASE COUNT         5181 a         5318 c         5658 g         5312 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 tgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttaca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac atgttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggtt
//
LOCUS       DAJTOW010000065        21245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTOW010000065 DAJTOW010000000
VERSION     DAJTOW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.065"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..793
                     /locus_tag="OO244_002569"
     CDS             <1..793
                     /locus_tag="OO244_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5664345.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="OO244_002570"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="OO244_002570"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5664346.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OO244_002571"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OO244_002571"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5664347.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OO244_002572"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OO244_002572"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5664348.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="OO244_002573"
     CDS             complement(4991..7225)
                     /locus_tag="OO244_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5664349.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OO244_002574"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OO244_002574"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5664350.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="OO244_002575"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="OO244_002575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5664351.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OO244_002576"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OO244_002576"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5664352.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="OO244_002577"
     CDS             complement(12980..14320)
                     /locus_tag="OO244_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5664353.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OO244_002578"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OO244_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5664354.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="OO244_002579"
     CDS             complement(16108..16557)
                     /locus_tag="OO244_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5664355.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OO244_002580"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OO244_002580"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5664356.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="OO244_002581"
     CDS             complement(17357..17686)
                     /locus_tag="OO244_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5664357.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OO244_002582"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OO244_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5664358.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="OO244_002583"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="OO244_002583"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5664359.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="OO244_002584"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="OO244_002584"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5664360.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5664 c         5073 g         5282 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgccttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaa
//
LOCUS       DAJTOW010000066        21167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTOW010000066 DAJTOW010000000
VERSION     DAJTOW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.066"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..923
                     /locus_tag="OO244_002585"
                     /pseudo
     CDS             1..923
                     /locus_tag="OO244_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1025..1177
                     /gene="hokA"
                     /locus_tag="OO244_002586"
     CDS             1025..1177
                     /gene="hokA"
                     /locus_tag="OO244_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5664361.1"
                     /translation="MPHKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1364..1576)
                     /gene="cspA"
                     /locus_tag="OO244_002587"
     CDS             complement(1364..1576)
                     /gene="cspA"
                     /locus_tag="OO244_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5664362.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1857..2147)
                     /locus_tag="OO244_002588"
                     /pseudo
     CDS             complement(1857..2147)
                     /locus_tag="OO244_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2581..3291
                     /locus_tag="OO244_002589"
     CDS             2581..3291
                     /locus_tag="OO244_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5664363.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3341..4315)
                     /gene="ghrB"
                     /locus_tag="OO244_002590"
     CDS             complement(3341..4315)
                     /gene="ghrB"
                     /locus_tag="OO244_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5664364.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4419..5069)
                     /locus_tag="OO244_002591"
     CDS             complement(4419..5069)
                     /locus_tag="OO244_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5664365.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5232..7565
                     /gene="bisC"
                     /locus_tag="OO244_002592"
     CDS             5232..7565
                     /gene="bisC"
                     /locus_tag="OO244_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5664366.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7534..7974)
                     /locus_tag="OO244_002593"
     CDS             complement(7534..7974)
                     /locus_tag="OO244_002593"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5664367.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7971..8534)
                     /gene="tag"
                     /locus_tag="OO244_002594"
     CDS             complement(7971..8534)
                     /gene="tag"
                     /locus_tag="OO244_002594"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5664368.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8692..9390
                     /locus_tag="OO244_002595"
     CDS             8692..9390
                     /locus_tag="OO244_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5664369.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9619..10827
                     /locus_tag="OO244_002596"
     CDS             9619..10827
                     /locus_tag="OO244_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664370.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            11149..12840
                     /gene="eptB"
                     /locus_tag="OO244_002597"
     CDS             11149..12840
                     /gene="eptB"
                     /locus_tag="OO244_002597"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5664371.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12932..13008
                     /locus_tag="OO244_002598"
     tRNA            12932..13008
                     /locus_tag="OO244_002598"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12966..12968,aa:Pro,seq:cgg)
     gene            13752..15359
                     /gene="dppA"
                     /locus_tag="OO244_002599"
     CDS             13752..15359
                     /gene="dppA"
                     /locus_tag="OO244_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5664372.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15667..16686
                     /gene="dppB"
                     /locus_tag="OO244_002600"
     CDS             15667..16686
                     /gene="dppB"
                     /locus_tag="OO244_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5664373.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16696..17598
                     /gene="dppC"
                     /locus_tag="OO244_002601"
     CDS             16696..17598
                     /gene="dppC"
                     /locus_tag="OO244_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5664374.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17609..18592
                     /gene="dppD"
                     /locus_tag="OO244_002602"
     CDS             17609..18592
                     /gene="dppD"
                     /locus_tag="OO244_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5664375.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18589..19593
                     /gene="dppF"
                     /locus_tag="OO244_002603"
     CDS             18589..19593
                     /gene="dppF"
                     /locus_tag="OO244_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5664376.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19623..20894)
                     /locus_tag="OO244_002604"
     CDS             complement(19623..20894)
                     /locus_tag="OO244_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5664377.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5140 a         5195 c         5522 g         5310 t
ORIGIN      
        1 caagttcgat ttcttgaaac gcggcttatg tatctaaaaa agctgaaagc cttagctcat
       61 cccacgaaaa agtgaaagta ctcaacgtgc taaggcagtt ttatcctctt gatgagcttc
      121 tcagggctgc ggagataccg cgcagtacgt tttattacca tctaaaggct ctcagcaagc
      181 ctgacaagta tgcggacgtt aaaaagcgta ttggtgagat ttatcacgag aatagaggcc
      241 gatacggata ccgtagggta acgctgtctc ttcatcgaga agggaaacag attaaccata
      301 aagctgttca gcgcctgatg ggaaccctct cacttaaagc agcgattaag gtcaagcgat
      361 accgctctta cagaggagag gtagggcaaa ccgcccctaa tgttctccaa agagatttca
      421 aggctacgcg gccaaacgag aagtgggtta ccgatgttac tgaatttgca gtcaatgggc
      481 gcaagctgta tttgtctcca gtaatagatc tcttcaacaa cgaagttatt tcttacagcc
      541 tttcggaaag accagtgatg aacatggttg agaatatgct cgatcaggca ttcaaaaagc
      601 ttaatcctca cgagcatcct gttctgcact ctgaccaggg atggcagtat cgtatgagaa
      661 gatatcaaaa tatccttaaa gaacatggta ttaaacaaag catgtccaga aaaggcaatt
      721 gtctggataa tgctgtggtg gagtgtttct ttggaacctt aaagtcggag tgtttttatc
      781 ttgatgagtt cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact
      841 ataacagcag aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc
      901 agacctatat gcctcgtgta taactgtcca actttttggg gtcagtacaa agtgaggcta
      961 ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac acagggggct
     1021 gggaatgccg cataaatata gattactttc tttaatagtg atttgtttca cgcttttatt
     1081 tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg agagttatga
     1141 gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg gaaattattc
     1201 ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt cacaaaaata
     1261 ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa aaaaatcccc
     1321 gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg ctggttacgt
     1381 taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt
     1441 ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca tctttagagc
     1501 catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt acgataccag
     1561 tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc ggcgatatgg
     1621 cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta tcaacgataa
     1681 ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa accgttgatg
     1741 tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt atgattaatc
     1801 ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat aaaagtctat
     1861 ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa ttttagttcg
     1921 gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac tgatacgccc
     1981 aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg ctcaatttcg
     2041 gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt aaaaacaatc
     2101 tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga aaactcctca
     2161 aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg ggaaaagcag
     2221 acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc tgtcatttag
     2281 cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat cactcctgtt
     2341 ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc tacaaaaaag
     2401 gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt cattttctcc
     2461 atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc tggactatcc
     2521 ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa
     2581 atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta
     2641 gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc
     2701 ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa
     2761 aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc tcagcaggtg
     2821 aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta
     2881 ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg
     2941 ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa
     3001 cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt aacgacgcca
     3061 gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca actggtgatg
     3121 gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa tggcatccag
     3181 ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt accggcacaa
     3241 catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata aagagtaaaa
     3301 gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg acgtgaggat
     3361 tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc acggcacagg
     3421 cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact gcgacgacgt
     3481 tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag acatccagcc
     3541 cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg
     3601 ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat tgttctgcgc
     3661 caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa tctgactctt
     3721 gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct tctttatggt
     3781 ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt tgtgccagcg
     3841 ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt
     3901 accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct gctacctcca
     3961 caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg gtttctgtta
     4021 atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg acatcaaaat
     4081 tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt tccagcaatg
     4141 cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa attgctgcat
     4201 tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca
     4261 ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc ttcatgcttc
     4321 tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga aaactgaaga
     4381 atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct taaggtaatt
     4441 tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat tgggttagcc
     4501 gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat caacgcgctg
     4561 gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg
     4621 tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac cattgccacg
     4681 ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc gaaggtcaca
     4741 ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac accagtgccg
     4801 cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac gccgccgccc
     4861 agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa
     4921 gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat agcagatttg
     4981 cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc cagagcgcca
     5041 ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt aataaccttt
     5101 ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg aagattattc
     5161 ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct aacctttcag
     5221 gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc gcccactggg
     5281 ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc gcgttagcca
     5341 caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc aatacgcggg
     5401 tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg caaggcattc
     5461 gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt attcaccaac
     5521 aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt tcctacggct
     5581 ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat atggcgctgg
     5641 caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag gcgatcatgc
     5701 cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg ctggtgctgg
     5761 aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg aaaattgcgt
     5821 ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac agtgggaaaa
     5881 agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc gataaaatgg
     5941 agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc gcccatacgc
     6001 tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg
     6061 tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc gaatgggcgg
     6121 cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa
     6181 ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt gagcaaaaac
     6241 actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc ggcggcggtt
     6301 ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct gcggtgctct
     6361 cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc cctgttgccc
     6421 gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt atgaaccgac
     6481 atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact catcatcagg
     6541 ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc tctgaatgct
     6601 tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc tcttttgagc
     6661 gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg atgaagcaag
     6721 tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag ttgagtgaac
     6781 gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg caatggctgg
     6841 aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat
     6901 ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc
     6961 ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta aaaaccgcca
     7021 gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg gattgccctg
     7081 ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca gaacagttgc
     7141 aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac agttctctgc
     7201 gcgaattgta cgcggtagca aaccgtgagc ctgtcactat tcatcctgac gatgctcagg
     7261 cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg cagatccttg
     7321 ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac gaaggggcat
     7381 ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga
     7441 ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg acgctggcat
     7501 ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca ccggccagct
     7561 cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc
     7621 ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca gcatcagatg
     7681 gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc tgacggcctt
     7741 cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa aaccgagaag
     7801 cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat cccgcaccag
     7861 cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg ttgtactttc
     7921 cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa tcatggttta
     7981 tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca tgcctgcata
     8041 aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag tgccttagat
     8101 agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc ttgtgtcacc
     8161 tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc gttctgttcc
     8221 atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg atggcggata
     8281 atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc gaccttcacc
     8341 ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac
     8401 gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt actgtcagtt
     8461 tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg actcacccag
     8521 ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc agccgctttt
     8581 cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc gatatccttc
     8641 atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata
     8701 aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat cagtgccttt
     8761 tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca acccggacat
     8821 tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga tattttgtcg
     8881 caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc ggaagaaact
     8941 cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata tgaacaggtt
     9001 acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat gtataacgag
     9061 tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt gagtagctta
     9121 ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat cagctataac
     9181 cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat gacggcgaca
     9241 aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct caatcaaaat
     9301 attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag cgactatctg
     9361 tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa ccattgaata
     9421 acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg tgaataattg
     9481 tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat ttcatgccgt
     9541 ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt tcctttttat
     9601 tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg gctgacactc
     9661 atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag cctgtttaat
     9721 ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc tttcggcttg
     9781 ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga acgttttggc
     9841 gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt cctgaccgcg
     9901 cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg tctggcggat
     9961 ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt
    10021 ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt caaatttatc
    10081 gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt
    10141 gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa acaggaagtg
    10201 aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg
    10261 cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct gtacgtgatt
    10321 ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc cgcagccaat
    10381 gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg cattctgtct
    10441 gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct ggtgggtatg
    10501 gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg
    10561 gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga gttcttcggc
    10621 ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat cggtagcatt
    10681 tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta cgtgattttc
    10741 gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga gcagaaaatg
    10801 ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc ttcagcttcc
    10861 gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaaag ggccagttaa
    10921 ctggcccttt ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta
    10981 aatttgtgct tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat
    11041 tcgcgcgtgt agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa
    11101 aagccgctaa gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc
    11161 aaatcgatta cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg
    11221 aatggcgcgg ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa
    11281 ggcgtttctg ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt
    11341 cttctatcgc tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt
    11401 tccgcaggtg ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc
    11461 gcttccgtca tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt
    11521 ctctggttaa tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc
    11581 tacaccttgc tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggtggtcgtc
    11641 gtactggcgg gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa
    11701 gtggagaggg cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat
    11761 ctgccatcaa actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct
    11821 tccgataata attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac
    11881 gttgatgata cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt
    11941 attttcggct atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg
    12001 ttccgtggtt actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt
    12061 caggggggcg cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt
    12121 ctgaagcagt taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac
    12181 agcaacacga tggcagacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat
    12241 cgtggcaagc cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc
    12301 aacccggatg gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc
    12361 cagcgttatc cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc
    12421 tgtaccaaag cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt
    12481 atctccagcg tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac
    12541 cacggtgagt caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg
    12601 ccggagcagt tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg
    12661 gccaatgcgc aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt
    12721 cacgtagagc tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga
    12781 attaacgaaa acaacaactg gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa
    12841 gcggcctgct gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg
    12901 cgtatctgcg cagtaagatg cgccccgcat tcggtgattg gcgcagcctg gtagcgcact
    12961 tcgttcggga cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc
    13021 ctgctcaacg agcaggtttt tttgcatttg tagctcatga ggatgagaac ctccgggggc
    13081 agggaggctc gactcgacgt aggcctgata agacgcgcca gcgtcgcatc aggcaacggc
    13141 accgcactta ttgtcggatg cggcgcgacc gccttattcg accaacacac cacacatttt
    13201 cctgccaact ttgcgcccca tatctcacta tccattaacg gatttgtgac agaaaccatt
    13261 gtcttttttt atatttgctt aaatcttttt tgcgttactt atggctggcg ctggcatttt
    13321 ccgctgtgcg ttttagcgtt catggtatta atcgctcaga tatccacctt ttcctcagat
    13381 ttttttcatt aaatgcataa aacttaatca ccaaatgttg ctaaatatta agctgattgt
    13441 tctggttatg ttggagtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt
    13501 aatctttgtt tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt
    13561 cacattctgt tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta
    13621 cactgatgtt aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa
    13681 caaaaaaggt caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt
    13741 ggagcagaat aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc
    13801 tggtggctat gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag
    13861 gatctccgga agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt
    13921 ccgtcccgct ttataaccgc ctggttgaat ttaaaatcgg caccaccgaa gtgatcccgg
    13981 gcctcgctga aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta
    14041 aaggtgtgaa gtggcacgac aataaagaat tcaaaccgac gcgcgaaatg aacgccgatg
    14101 acgtggtgtt ctcgttcgat cgtcagaaaa acgcgcaaaa cccgtaccat aaagtttctg
    14161 gcggcagcta cgaatacttc gaaggtatgg gcttgccgga gctgatcagc gaagtgaaaa
    14221 aggtggacga taacaccgtt cagtttgtgc tgactcgacc ggaagcgccg ttcctcgctg
    14281 acctggcaat ggacttcgcc tctattctgt caaaagaata tgctgacgcg atgatgaaag
    14341 ccggtacacc ggaaaaactg gacctcaacc caatcggaac cggtccgttc cagttacagc
    14401 agtaccaaaa agattcccgt atccgctaca aagcgtttga tggctactgg ggcaccaaac
    14461 cgcagatcga tacgctggtc ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat
    14521 tgcagaagaa tgaatgccag gtgatgccgt acccgaaccc ggcagacatc gctcgcatga
    14581 agcaggataa atccatcaac ctgatggaaa tgccggggct gaacgtcggc tacctctcgt
    14641 ataacgtgca gaaaaaaccg ctggatgacg tgaaagttcg ccaagcacta acctacgcgg
    14701 tgaacaaaga cgcgatcatc aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc
    14761 tgatcccgcc aaccatgtgg ggctataacg acgacgttca ggattacacc tacgatcctg
    14821 aaaaagcgaa agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt
    14881 gggcgatgcc ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc
    14941 aggcagactg ggcgaaagtg ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt
    15001 acctcaagcg tgcgaaagat ggcgaacatc agacggtaat gatgggctgg actggcgata
    15061 acggggaccc ggataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt
    15121 ctaactactc aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta
    15181 ccgacgacca taacaaacgc gttgaactgt acaagcaagc gcaggtggtg atgcacgatc
    15241 aggctccggc actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtca
    15301 aaggctatgt ggttgatcca ttaggcaaac atcacttcga aaacgtctct atcgaataat
    15361 taaaagccat acaagactga tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg
    15421 cctacgaaaa ttctgcaata tattgaattt gcacgatttt gtaggccgga taaggcgtta
    15481 acgccgcatc cggcataaac aaagcgcact ttgtcaacaa tctgtatacc cggtggcgct
    15541 gtgcctctgc gtgcagcgcc atccggcagc aatacttcat tctctgtccg acaagggcgg
    15601 gaattgattt gtgagcaata cagacacgca gttccaggct gcgagtcact atagagaatc
    15661 cgggttatgt tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt
    15721 attacccttc tcacatttgc ctttgtccac atgatcccgg gcgatccggt gatgatcatg
    15781 gcaggcgaac gtgggatctc ccctgagcgt cacgtgcagc tgctggctga actcggctta
    15841 gataaaccga tgtggcagca gtatctccat tacatttggg gcgtaatgca cggcgatcta
    15901 ggcatttcaa tgaaaagccg aattccagta tgggaagagt tcgtgccgcg ctttcaggcc
    15961 acgctggaac ttggcgtctg cgcgatgatt tttgctacgg cagtcggtat tccggttggt
    16021 gtgctggctg cggttaaacg cggttccatt ttcgatcaca ctgcagttgg cctggcgctg
    16081 accggctact cgatgccgat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg
    16141 cactggaacc tgacgcccgt ctccggtcgc gtgagcgata tggtgttcct cgatgactcc
    16201 aatccgttaa ccggttttat gctaatcgac accgccatct ggggtgaaga cggcaacttt
    16261 atcgatgccg tcgcccatat gatcttgccc gccattgtgc tgggtactat tccgctagcg
    16321 gtcattgtgc gtatgacacg ctcctcgatg ctggaagtgc tgggcgagga ttacatccgc
    16381 accgcgcgcg ccaaagggct aacccgcatg cgggtgatta tcgtccatgc gctgcgtaac
    16441 gcgatgctgc cggtagtgac cgttatcggc ctccaggtgg gaacattgct ggcgggggcg
    16501 attctgaccg aaaccatctt ctcgtggccc ggtctggggc gctggttgat tgacgcactg
    16561 caacgccgcg actatccggt agtgcagggc ggcgtattgc tggtggcgac gatgattatc
    16621 ctcgtcaact tgctggtcga tctgctgtac ggcgtggtga acccgcgtat tcgtcataag
    16681 aagtaagggg acatcatgtc acaggttact gaaaataaag tgattagcgc accggtgccg
    16741 atgaccccgt tacaggagtt ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg
    16801 ctggtttacg tcgccatcgt gctgttcatc gcgatctttg ccaactggat tgcgccttat
    16861 aacccggcgg atcagttccg cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc
    16921 agtatggcac acttgctggg caccgatgac gtcggtcgtg atgtgctgtc gcggctgatg
    16981 tacggcgcgc gtttatcgct gctggttggc tgtctggtgg tggtgttgtc gctgattatg
    17041 ggcgttattc tcggcctgat cgccggttac ttcggcggcc tggttgataa cattatcatg
    17101 cgcgtggtcg atatcatgct ggcgctgcca agtctgctgt tggcactggt gctggtggcg
    17161 attttcggcc cgtcgattgg taacgccgcg ctggcgctga ccttcgttgc cttgccgcac
    17221 tatgtgcgct taacccgcgc cgccgtgctg gtggaggtga atcgcgatta cgtcaccgcc
    17281 tctcgcgtgg caggtgccgg ggcgatgcgc cagatgttta ttaacatctt cccgaactgc
    17341 cttgcgccgc tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct
    17401 gctcttggtt tcctcggcat gggggcacag ccgccaacgc ctgagtgggg caccatgctc
    17461 tccgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc tggtctggcg
    17521 atcctgttga cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat
    17581 cccaaactaa agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt
    17641 gcatttcggc gacgaaagcg caccgttccg cgccgtagac cgcatcagct acagcgtaaa
    17701 acagggcgaa gtggtcggga ttgtgggtga gtccggctcc ggtaagtcgg tcagttcact
    17761 ggcgattatg gggctgattg attatccggg ccgcgtaatg gcagaaaaac tggagtttaa
    17821 cggccaggat ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg gtgccgaagt
    17881 ggcgatgatc ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca
    17941 gattatggaa gcgattaaag tgcatcaggg cggcaacaaa agtacccgcc gtcagcgggc
    18001 gattgacctg ctgaatcagg tcggtattcc cgatccggct tcgcgtctgg atgtttaccc
    18061 gcatcagctt tccggcggca tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg
    18121 gccaaaactg ctgattgccg atgaaccgac caccgcgctg gacgtgacca ttcaggcgca
    18181 aatcatcgaa ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac
    18241 ccatgacctg gcgctggtgg cggaagctgc gcataaaatc attgtgatgt atgcaggtca
    18301 ggtagtagaa acgggcgatg cacacgccat cttccatgcg ccgcgtcacc cgtatactca
    18361 ggcattgctg cgtgcgctgc cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc
    18421 aggtgttgtt cccggcaagt atgaccgccc gaacggctgc ctgcttaacc cgcgctgccc
    18481 ctatgccact gacagatgtc gcgctgaaga accggcgctg aatatgctcg ctgatgggcg
    18541 tcagtccaaa tgccattacc cacttgatga tgccgggagg cccacactat gagtacgcaa
    18601 gaggccacct cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg
    18661 aagaaaggca tgttcgtgcc ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac
    18721 cttgaacgtg gcaaaacgct ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc
    18781 ggtcggttgc tgacgatgat cgaaacgccc accggcggcg agctgtatta tcaggggcag
    18841 gatctgctca agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc
    18901 ttccagaatc cctacggttc gctgaatccg cgtaagaaag ttgggcaaat tctcgaagag
    18961 ccgctgctta ttaacaccag cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg
    19021 atggcaaaag tcggcctgaa aaccgagcac tatgaccgct atccgcacat gttttccggc
    19081 ggacaacgtc agcgtatcgc tattgcccgt ggtctgatgc tcgacccgga tgtggtgatt
    19141 gccgatgagc cggtttctgc gctggacgtc tcagtgcgtg cgcaggtgct gaatctgatg
    19201 atggatttgc agcaggagtt ggggctttct tatgtcttta tctcccacga cctgtcggtt
    19261 gtggagcaca ttgctgatga agtgatggtg atgtacctag gccgctgcgt ggagaaagga
    19321 acgaaggacc aaatcttcaa taacccgcgc catccgtaca cccaggcgct gctctccgcg
    19381 acgccacgcc tgaacccgga cgatcgtcgt gaacgcatca aactcaccgg tgaactgcca
    19441 agcccgctga atcctccgcc gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc
    19501 ccctgcaccc agttgcagcc gcagctaaaa gactacggag gtcaactggt agcttgtttt
    19561 gctgttgatc aggatgaaaa tccgcagcgt taactcacaa aaccggggcc atgcgctccg
    19621 gtttactcaa tcagcttcaa cagtggcgac aagcatagca aaatgccgta aagcagaatc
    19681 agccaggctt taaatccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaac
    19741 ggaatgaggc acgacacaat gccatataac gggcttccca actgaaagaa caccagcact
    19801 gatacacgaa acgaaaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga
    19861 gtcagcatgc gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata
    19921 atgcctttaa tagcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca
    19981 aagatattca gcaccgtaga ggtgatatga atgatatgtc cagggatcac ctgcgcggcc
    20041 agtgccagtg ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag
    20101 aaggtaaacg aaaaggcaaa aaacaggatc accgcgatga gcgtgatata actaattcgg
    20161 tgggtacgca gcgcgagccg ggttgccagt actttatctg cttcccgttt acgataggca
    20221 atattcattg ggttaagtac ctgaataaat accgcagaaa agaagcaaaa tggaatggta
    20281 agcaggacat cgcggaaaaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc
    20341 cagtgcggga tcatcgcaaa accgaacacg acaataatcc ctactttgac caccaccatt
    20401 ggcccagaaa tcttaaacag taatcgttca ccaccagacg caatcgctat cagtacggcg
    20461 aaaatagcga ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt
    20521 ttcaggtacg aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata
    20581 atcatcaaaa agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg
    20641 ataatatcgg tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc
    20701 tgcactaccc aggtgacagg ataagcaatg atcgctgcgg taataaatac ccaaattccc
    20761 ttcaagccaa tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat
    20821 aataaaaccc agccgaagtc gtagcgtgta aacggcaatt tctgattgtg tttcgatagt
    20881 gtgttgtgct gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc
    20941 cagtttctct tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa
    21001 cagattcact ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc
    21061 agtctggagt cagcccggat atgaactgca actattgcaa ataaagaaat taagctggaa
    21121 ggcatcgcgc aatgaggaag attgccgggt gctgacagta aaaagca
//
LOCUS       DAJTOW010000067        20813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTOW010000067 DAJTOW010000000
VERSION     DAJTOW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.067"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            42..623
                     /locus_tag="OO244_002605"
                     /pseudo
     CDS             42..623
                     /locus_tag="OO244_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            641..1279
                     /locus_tag="OO244_002606"
     CDS             641..1279
                     /locus_tag="OO244_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5664378.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDDDKPFFM"
     gene            1570..2298
                     /gene="mpaA"
                     /locus_tag="OO244_002607"
     CDS             1570..2298
                     /gene="mpaA"
                     /locus_tag="OO244_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5664379.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2273..3238)
                     /gene="ycjG"
                     /locus_tag="OO244_002608"
     CDS             complement(2273..3238)
                     /gene="ycjG"
                     /locus_tag="OO244_002608"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5664380.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMVQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3357..3863
                     /gene="tpx"
                     /locus_tag="OO244_002609"
     CDS             3357..3863
                     /gene="tpx"
                     /locus_tag="OO244_002609"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5664381.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3907..5448)
                     /gene="tyrR"
                     /locus_tag="OO244_002610"
     CDS             complement(3907..5448)
                     /gene="tyrR"
                     /locus_tag="OO244_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5664382.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5596..6657)
                     /locus_tag="OO244_002611"
     CDS             complement(5596..6657)
                     /locus_tag="OO244_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5664383.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6654..8051)
                     /locus_tag="OO244_002612"
     CDS             complement(6654..8051)
                     /locus_tag="OO244_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5664384.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFHRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8207..9205
                     /locus_tag="OO244_002613"
     CDS             8207..9205
                     /locus_tag="OO244_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5664385.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9316..10188)
                     /gene="ompG"
                     /locus_tag="OO244_002614"
                     /pseudo
     CDS             complement(9316..10188)
                     /gene="ompG"
                     /locus_tag="OO244_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10261..11342)
                     /locus_tag="OO244_002615"
                     /pseudo
     CDS             complement(10261..11342)
                     /locus_tag="OO244_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11356..12015)
                     /gene="pgmB"
                     /locus_tag="OO244_002616"
     CDS             complement(11356..12015)
                     /gene="pgmB"
                     /locus_tag="OO244_002616"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5664386.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(12012..14279)
                     /gene="ycjT"
                     /locus_tag="OO244_002617"
     CDS             complement(12012..14279)
                     /gene="ycjT"
                     /locus_tag="OO244_002617"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5664387.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14276..14899)
                     /locus_tag="OO244_002618"
     CDS             complement(14276..14899)
                     /locus_tag="OO244_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5664388.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14896..15318)
                     /locus_tag="OO244_002619"
     CDS             complement(14896..15318)
                     /locus_tag="OO244_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5664389.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15340..16115)
                     /locus_tag="OO244_002620"
                     /pseudo
     CDS             complement(15340..16115)
                     /locus_tag="OO244_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16133..17185)
                     /locus_tag="OO244_002621"
     CDS             complement(16133..17185)
                     /locus_tag="OO244_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5664390.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17216..18058)
                     /locus_tag="OO244_002622"
     CDS             complement(17216..18058)
                     /locus_tag="OO244_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5664391.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18045..18926)
                     /locus_tag="OO244_002623"
     CDS             complement(18045..18926)
                     /locus_tag="OO244_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5664392.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18947..20230)
                     /locus_tag="OO244_002624"
                     /pseudo
     CDS             complement(18947..20230)
                     /locus_tag="OO244_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20244..>20813)
                     /gene="ycjM"
                     /locus_tag="OO244_002625"
     CDS             complement(20244..>20813)
                     /gene="ycjM"
                     /locus_tag="OO244_002625"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5664393.1"
                     /translation="KNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAILLS
                     FPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVYHE
                     LSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVNIT
                     DLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5084 a         5673 c         5063 g         4993 t
ORIGIN      
        1 ctggctaaca ccccattata aggggtacga ataaagtaac gggtggctat gccattaatg
       61 ccgcgctgac agaaaaacga attaaagctg tagtgagtat tacgggtgtt aatatcgggc
      121 gtctttttcg ggaaggtttc agcaattatg atccgattgg cgtattgaat gcaatggcgt
      181 cacaacgagc aaaagaggct cgtggtggag aattacagat caacgaatta cttcccgcca
      241 gcctggatgc agcaaaagcc catggactga ccgaacgcga tgtttatgaa gcaactgact
      301 actataaaac accacgcggg caacaaccgg gaggtgcgac aaaaatgttg ttctcacatg
      361 cacagaaaac gctggcctgg gatgccttcg catttactga agtgttactg acccagccgg
      421 tgatggtagt agttggtgaa aaagtcggtg ccttcggtgc ttatcgtgat ggactggaag
      481 tctatggccg ggcgatggtt tcgcaggatc gtcagttagt ttcgttaccc gatttctcac
      541 attatgaact atatgacaaa ccggaagcag ttcaggaggc gctggcaaaa gttattcctt
      601 ttttcaacac ccatctggga taagtttcat gagaaatatt atgaaaaatg tactgattct
      661 tggtgctggc ggtcagatag cccgccatgt gattaatcaa ctggcagata aacaaactat
      721 taaacaaact ttatttgccc gtcagccagc aaaaatacac aaaccctatc caactaatag
      781 ccagattatt atgggggatg tactgaatca tgcagcactg aagcaggcca tgcagggaca
      841 ggatattgtg tatgccaacc ttacggggga agatttagat attcaggcaa attcagtgat
      901 cgctgcgatg aaagcctgtg atgttaaacg tctgatcttt gttctttccc tcggtattta
      961 tgatgaagtt ccaggaaaat ttgtcgaatg gaataatgca gttattggtg aacccctaaa
     1021 accattccgc agggctgcgg atgcaattga ggcttctgga ctggaatata caattcttcg
     1081 ccctgcctgg ctgactgatg aagatattat tgattatgag cttacctctc gcaatgaacc
     1141 attcaaaggt acgatcgtat cacgtaagag tgtcgcagca ttaattacag atatcatcga
     1201 taaacctgag aaacacattg gtgagaatat aggcatcaat caacccggga ctgatgacga
     1261 taagccattc ttcatgtaaa tcaattatat agataccatc gtactgtact atgtgcagta
     1321 cgatttatta aacttattaa ggtgaactga tcaatcagat gcgattaaca tgtgtagaac
     1381 cagtaagaat aaaatacgcc atgtatataa cattacgaca attctaatat ctgcacatca
     1441 atgaaaatgc aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca tagcgttgaa
     1501 gaaaaggatt tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca
     1561 gagacgatca tgaccgtaac ccgctcacgc gccgaacgcg gcgcatttcc gcccggaaca
     1621 gaacattacg gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc
     1681 agccgcgaaa gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc
     1741 acgctctcct gtgctctgcg gactttgaca ccttctttgc gccgccatca tgtggtgctg
     1801 tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca atgctaatgg tgtggattta
     1861 aacagaaact ttccggcggc gaactggaag gaaggtgaaa cggtttatcg ctggaacagc
     1921 gccgctgaag aacgtgatgt cgtattactg acaggtgaca aacctggctc cgaacctgaa
     1981 acccaggcgc tgtgccagct tatacaccgc attcaacctg cctgggtggt ctcattccac
     2041 gatccactgg cttgtattga agatcccaga cacagcgaat taggcgaatg gctggcccag
     2101 tcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt
     2161 tggtgtgcag atctcaactt gcattgcatc accgcagagt ttccgcccat ctcctccgac
     2221 gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat
     2281 gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc
     2341 cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag
     2401 aggtacacaa catgcagccc agcatcagac tgaaaccttg tgcacgcgct tcagtcgcca
     2461 gcgccagcgc ttccgtcaga ccaccggttt tatcgagctt aatgttaacc atctcatagc
     2521 gccctttcag cgccttcaga ttgctacgag tatgacaact ttcatcagca caaatcggca
     2581 acggatgaat aaaattctcc agcgccgcat cgtcctgcgc aggaagcggt tgttcaagca
     2641 tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg
     2701 attcatttgc atcaacgatc agcgtggcat cgggcacagc ggtgcgaatt gccaccatcc
     2761 gctcactgat aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga
     2821 gtgttgatgc actattggcc atctgatcag gcgtaccgat gacaaccgtc tgtgcagtga
     2881 taactgtctc gggaagcgtt atgccgatca aatcagccag cgattgctgc tgttttcgcg
     2941 ccgcgagatc ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt
     3001 tttgcaactc ctcccgtgtc agccctttct ctaattgcgg cacgacgctc ataatttgca
     3061 ccattaccga ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt
     3121 taataccctc ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccggg
     3181 caatcacaaa cggggtatgt aagggccagg cttcctcaaa taccttaacg attctcatct
     3241 ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt
     3301 gacagttagc ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt
     3361 cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg
     3421 gtagcaaagc gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc
     3481 agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg
     3541 ccgcatcagt acgtaagttt aaccaactgg caactgagat cgacaacacc gttgtgctgt
     3601 gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca
     3661 acgttatcac cctctccact ttccgtaacg ctgaatttct gcaagcctac ggtgtggcaa
     3721 ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt gacgaaaatg
     3781 acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag
     3841 ctctggctgt actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat
     3901 tcgcgcttac tcttcgttct tcttctgact cagaccatat tcccgcaact tattggcaat
     3961 cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata
     4021 aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg aaccttccat
     4081 cgcatcttcg cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa tatcctgtgg
     4141 acgcagctca taaccgtcca gttgtgtcag tgcgcgatag atagcgttct ttaactgccg
     4201 cacatttccc ggccacgcat aacgtgtaag tacagtattc aggtcagcgg ccagtttcgg
     4261 acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta acggcataat
     4321 gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata
     4381 gagatcttca cggaacacgc ctttttgcac cagttcgacc agattcttct gcgtagcgca
     4441 aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga aagtgccatc
     4501 attaagaaaa cgcagtaatt tcgcctgcat ccgtggtgac atttccccta tttcatccaa
     4561 cagcaccgaa ccaccgttcg cctgctcaaa gaaccctttc ttcccttccg gagcatgacc
     4621 aaacagttca ctctcgaccg catcttctgg tatagacgcg caattcagcg ccaggtaagg
     4681 tttgcccgct ctggggcttg cctgatggca ggcgtaggca aaaagatctt tacctgtacc
     4741 tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg cctgttcgac
     4801 aacgtgcttc attttcgggc tgacggcgac aatttggctg aaggcgctga cgtcctgggc
     4861 ggcgacattt tgcaactggc ggcccatacg aatcgttgat cgcaacatca ccaccgcacc
     4921 ggtcaggacg tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa
     4981 attctgccca ttaataacga catgctcgtt atgcgaatct tgcggttcgc tttccagcca
     5041 acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta tggttgcgca ggcgatccaa
     5101 tttttgccca aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc
     5161 gacagagagc acaggttcag gcaacgcctc cagtaacgcg ctcaacgcca gatgctcacg
     5221 ttcggaaggc atccacggga cagtacgcac atcggtaaca cctgcaatac ggcgtatttc
     5281 ggccatcaga ctgctgaaac tctcaaactc cagttcagca aaattgaggt agattcgccc
     5341 aatgggatca atctcaatac cgcgtaaatc aatgcctctt agcacgagta gatcgagtaa
     5401 ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac
     5461 ctgaaaaaag aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg
     5521 tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca
     5581 gcacccgata ttgcattatt tttcgctggg cgttttgcct ttttgcagag tttctttcac
     5641 ctgaccgata agctgacgac ggaaatcccc aaggcgaggt ttgtcatcgt caatccacgg
     5701 cagcgggcgg caaagctcca tagctttaat cccgagtcgt accgtcagaa gtccagcacc
     5761 aattccctga gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca tccagtccat
     5821 ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg atattcagca ataccagctt
     5881 aaacagacgc aaacggctgt aataccccag ttcaatgcca tacagcgtgg caatgcgatt
     5941 aattaaacgc agattgcgcc aggcgataaa cgccatatcg accaacgcca gcgggctgac
     6001 cgcaatcatc aacgttgatt ccgccgccga acggctgatt tcgcgccgcg cctgggcatc
     6061 taaaactggc tggaccaaat gcgcatacag gctgaccact tcacgatcgt tctgcgtttc
     6121 atggattgag gcataccagc gttgcagcgc cggatgcgac tgatcaatac ccgcctgctg
     6181 cgccagtttt tcgcaaaatg cgcgaccttt gcccgtgccg tggctatgca ataaatcacg
     6241 cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac
     6301 cacagaacct acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgctac
     6361 ccagtcctgg gtttgccagg cattcattgt ccactgtata ccctgcccga caacgcttgc
     6421 gccaaacaga gccagcccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa
     6481 cgctgcgtcc attaccgctt cgacttgccc ctcttcttcc tgcgcttcgt cgagcgtggc
     6541 cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt
     6601 ctgttcgacc tccagcggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc
     6661 aatttatctc ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg cagcggtttg
     6721 tcgacatcca tcacctgcgg gcgaaacgct tcaaactgga acccttgctt gtcccagaac
     6781 gcctgaccgg gcaaacgcgc aggaacttca ccggggtaaa cggtgagagg cgcaccatcg
     6841 ctgagccggt taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat aatgccgctg
     6901 gtggtcgcct gcaccgaggc cagccccagg caatccatac taatcctttc aaacgccgca
     6961 ttttgccagg catcctgaat cagttgttgc agtaatgaaa ccatattggc gtgctgatcg
     7021 accgttacat ggtccgcttt catcgcggca aacagcagtt tgtcgataac cggcgagaat
     7081 aagcggtgaa acagcgtgcg ctgcccgtag tggaaacttt gcatcagctg cgtcagtgcc
     7141 agacgcatat cattaaatgc ctgtggccca ctgttgagag gttgcaggca atccaccagc
     7201 acaatctggc ggtcaaagcg cagaaaatga ttcttataga accccttcac cactttctcg
     7261 cagtaataat taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc ggcctgcgcc
     7321 agtttggact cgtcccaggc atcgacatcc ggccacggga agaattgcag cgcgggcgca
     7381 cctgccatat ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcag cccctgctgt
     7441 ttacagtggt ggagataatc ggtccacacg gcggcaatgt ctgccagacg gttttcgtcg
     7501 gcaggcgcta gcgggtcaag tccttcgcac atcattcgcc atttcgccga ccattcgccg
     7561 cgctgaccat tgagtaagcc cgtcatctgg cgcgaccagc ttaagtaatc ctgcgccagc
     7621 atcggcaggt cgagcaacca ttcgccggga taatccacaa tttccagata cagcgtggag
     7681 gtatccttaa agtggcgcag cagcgaatcg ttcgatttaa aacgtaacgc caggcgaatt
     7741 tcactgacgc cgcgcgtagg cgtcggccag gcgggaggat caccatataa ctgcgccagt
     7801 ccttcatcat aggtgaagcg cggaatgcca aagtcacgct ggggaatgcg ttttacgccc
     7861 agcaagcgct cttcacgcac cgcgcttaac agcggcaaac gtgctccggc atgaatattg
     7921 agcaactgat taaccatcgc ggtgataaac gctgttttgc cgctgcggct aagtccggtt
     7981 acagcgaggc gcagatgtct gtcgacaccc cgattcacca gcgcattgag ttcattttta
     8041 agtcgcttca ttgctgtcct gtgctgctct gaagaccaga aaaaaaatca ttcgtaagga
     8101 tatgaatgtg ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa
     8161 ctccataaga tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat
     8221 ttatgatatt gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa
     8281 gcaaactaat atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca
     8341 gtatcagcca aacaagctgg cccgcgcgct gacctcttcg ggttttgatg ccattatggt
     8401 gatttctacc cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca
     8461 tgccatcacc gccaaagcgg aagaagaagg tttcgacgtg atattacaaa cgtcgcacaa
     8521 tccggcagaa gatctgcaaa aatgcgaaag caaaattaaa cagaaaatga ttaaaggcat
     8581 tattatgctc agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat
     8641 tccggtggtg gtaattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac
     8701 cgataatttt ggtgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa
     8761 tattgcctgc ctgcatgctc cgcttgatgt ccatgtttcg gttgatcgcg ttaatggcta
     8821 taagcaaagt ctgtccgcgc ataatattgc agtgcgtgat aaatggattg ttgacggcgg
     8881 ttatacccat gaaacagcac tgcaagcagc acggcaatta ttaagtcagt caccgttgcc
     8941 tgaggcagtg tttgccactg acagcctgaa attaatgagc atttatcgtg cggcggcaga
     9001 gaaaaatatt gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc
     9061 gtttatttta acgcctgcac caggcggcat cgatgttccg acgcaggagt taggtcaaca
     9121 aagctgcgag ttattattcc gcttaatttc gggaaaaccg tcaccacaga atattaccgt
     9181 tgccacgcat atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg
     9241 ctggcgcatc ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg
     9301 caatttagcc cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc
     9361 gctgtcgcct tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc
     9421 gttctggaaa tcataaccgt aaaataaacc cacacggtta aaatcatggc cttcacgttc
     9481 aatatcatcc tgccagtccc agttactcta gcgatccagc ccaatgcgcg tatacggcgt
     9541 caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga atttcttgtg tggaaaactc
     9601 accgttattg cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa
     9661 agcaaccgtt tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc
     9721 gtaaccggtc gtgttcaaat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg
     9781 taaatcatca gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc
     9841 cgtgtctttg cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag
     9901 gccgaaactg aaatcatcgc tttcgaggaa ctgataatgc acctccagct ccgggcgatc
     9961 aaacctcgtt ccacgtttac ccgcactata atctaccggc ccttcctgat aataggccag
    10021 agcaattctc cacggcccgt tggcggcatt aaaatagact gaaggctccg ccagcccatc
    10081 catatcttcg ccataaccct cgacgttttc tatttcgtac atcgcaccga tattaaagtg
    10141 ccagtcgctc ttttcctcgg cctgtgcgca ggccattccc gcacacatca ccagtgcggt
    10201 acagggtaat aacttttttt gttccttgta ttatcctggg ggaatgcctt ccccctgtat
    10261 ttagcgaatt gctatttccg tttctgcatc aaagaaatga catttcgtca tatcaaaatg
    10321 aatagtgata ttttctcctg catgataatc atttaacgca ccagcacgga ccactaactc
    10381 gtgcccccaa ccgtggtgta gagcataaat tccgcaccgg ttaattctgc cacgctaatt
    10441 ttggcggaaa tgttattttc ctcttgcgcg tccggatgaa tatcttccgg gcgtattccc
    10501 atcacgatgg gcttatgcaa actttcctgt gttttcagaa ccgctaattt ctcttcgaga
    10561 atggttaatt taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc gcgaataaaa
    10621 ttcatcgctg gtgatccaat aaatccggca acaaacatat tcgcgggttg gttataaacg
    10681 gttttcggcg cacctacttg ctgaacaatc ccgtctttca taatcacaat ccgcgccgcc
    10741 atggtcatcg cttcggtctg atcgtgggtc acgtagatca tggtggtgtt cagtttctga
    10801 tgcagcttgc tgatctctgc acgcatttgt acgcgcagct tggcatcaag gttagagagc
    10861 ggttcatcca ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag cgccacacgc
    10921 tggcgttgcc cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg caggccgaga
    10981 atttgcgccg cccagttcac ccgctcatca atcacctctt tggcgatttt ttgcatcttc
    11041 aggccaaacg ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta gttctggaac
    11101 accatcgcga tattgcgtgc tttggctgga acgtcattca ttcgtttgcc gtcgatcaac
    11161 agatcgccgc cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt cgatttaccg
    11221 cagcccgacg ggccgacaaa tacgatgaac tctttatcgg caatttccag gttgaagtcc
    11281 ttcaccacat gcacctggtt atcgtagatt ttttgaatat gttgtaacga aagctgagcc
    11341 atgttgattc ctttgctata cgttttgcca gaaggccgat aaccgcgacc aggtcaggga
    11401 gtccgttgag ggcaacagta attgcgcccc ggttaagccc gcaccgatcc ccaccgagcg
    11461 cataccgctg gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc cgatacatgc
    11521 ctgcggcggc acgcccagcc cggcacaggc agcgagaaag atttccggat ccggtttcga
    11581 gtttttaagt tgggaagcat ccgcgcagaa ggtgaaaaac tcacgcagct ccagcgccgc
    11641 taaaatcgtc ggcgcattca gggagacgga agccagccct accggaatct gctgtgcacg
    11701 gagctctgcc agcaaattgc gaataccggg cagcacagca ttaaccgtca gctcgcgcag
    11761 tgagtggacg taaagcagat ttttgcgata cgccagttgc gcccgctcct gcgggttaaa
    11821 gtcgccctct ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc ggctgatccc
    11881 ttttagagat tcgttaaact gcgcatcaat actgatgcca atttcagcgg caatctgctg
    11941 ccacgcctgg aaatgcagat gcgcggtatc ggtgattaca ccatccagat cgaaaattac
    12001 cccttgcagt ttcattcatc ctcctgatgc gtggtagcgg tcccattgaa gggcaaaata
    12061 aaatcaccca aacagaaaac agattcttca gccacggata taagctgacc gtccaaacgc
    12121 agtgaaacgg gcgcagaagt tcgaatcgca atacgctgcg cgtcgagagt gacctgtaat
    12181 tcgcagccct gccagaacag agggaaagac aactgttgcc actgctcaag taacgccgga
    12241 tttagatgca attcaccgtc acgcacgctc accccggcaa aaccctgaat cgcccccagc
    12301 cagatagcgc cagttgcggc agcatggata ccatcatcac aactatgcgg atcagcacca
    12361 agatcgattt cagtcccctc gcgccagaac tgataacttt gggtcagcag gccacagcgt
    12421 gcagcaacaa tgccgtggat tgctttactt aatgacgagt cgtgaatagt gcgcggttca
    12481 taaaattgca gattggcaag acacgatgcc gatgagaact gctctggcag catgtaattg
    12541 agcatcacca catcagcttg tttgaggatc tgcatctcgt tcacttctgc gcgtgaataa
    12601 tccagcagaa tggtttgctt ccccgccgcc gctttgtatt tcgccagatt aatcgccggc
    12661 ttagtcataa acgaatcatc ctgcggcaaa acaccgtcgg gctgaatttc tggcagccgc
    12721 agttctttaa ggaacatttc ggcacgatga ataaacgcgt cgtcactaca gccaaactgg
    12781 cgggcaatac tcagcgcctg ctggacgttg taatacacca tatagctggt gaaggcgtta
    12841 ttattgacat gttcggtata ttcgtctggc ccaataacat catgaatttc cagacggtcg
    12901 ttaaccctca ccgcgcggct aatccagaac tttgcagttt ccagaagtag cgccatgcct
    12961 tcatgcgcaa tgaaactttc atcccccgtg gtctgccagt attgaataac cgcccaggcg
    13021 atatcggcca ccagatgatg ttccgcctgc gccgaggcca ctttttgccg cagcccggtg
    13081 cgaatgttaa tggcggcaaa ttccggcgtc tcttcttcgc cgctgcgcgc gctttcccac
    13141 ggaaatagcg cgccctgcca gccgttgcgt cgcgctttct cctgcgcgcc tggcaagttg
    13201 tgccagcgat aacgcagtaa acttcgggca accgtcggat cgctaaacag atggaacggc
    13261 agcaaaaata cttctgtatc ccagaaaacg tggcctttgt agccttcgcc ggttaagcct
    13321 tttgccgcaa tactgctgcg ctcgccgtga gccggcgtca tgatgcgcag atgataaagc
    13381 gcataatcta acgcttgctg atcgtgcgca tcgccgccat ttaccgtgat acggcgtttc
    13441 tgccaccatt gacgccagtt ttctgttgat gctgcaagat gttggtcgta actctgttgc
    13501 gcgcacattt caagctggcg aagcgacacg ctgccccact cgtctaaagc agcttgcctg
    13561 tcatcccgcc agtcgatcca caccagtttt tgcaacgtca ctgtctcgcc tgcatgaagc
    13621 tgcgtactgg tatgttgcag taaacggcgc tctttggcgg tatagcattg ctgcacatca
    13681 ccgctcacct ggcagcaaca actgatggcc acatcactgc gcccatcctg ggtggtgtag
    13741 atcccctgca tcaaatgctg accaaacacc cgcacctggg tttcgtcgag atgctggcga
    13801 ccgtggttgg tttgcgtggc gtcaatgcct gttgaaatca gcactgacgc gtcggcgtcc
    13861 agtggcataa tagtgatttc cagcgcaatg agcggcagtt ggtctgccga aacaaagcga
    13921 cggctggcga tggtgtaacc tgcgccgttg ctggtacgcc agacaacgct acggcgtaat
    13981 tctccactgg caaagtcaag ttcacgctgc caggcttcgc gggataacga gaaaacctca
    14041 ccatttatgg caatctccat ccccaagata tcaggcagat tcaccagttc gttgatttca
    14101 ccttttcccg cccgatgata cagccccgcc agatacatcc ctcgcgtctg gcgcgtgtaa
    14161 tcttcttcat ggctggcgcg aagcccaaga tagccgttcc cctgcgccat cagcgatgca
    14221 tacttgttca gggtatgctg gctgaaatgg ggttctgata acgttactgg cctggtcata
    14281 attccacacg cgtccctgtt tctgcggatt gatataacgc cgccaccagt tgctggatga
    14341 tgtacccctg ctcggcgtcg gctatcatca cgggcttgcc ctgtacgtga ttgataaagg
    14401 cttccatgct gcgcaaatgg cggttgtcgt ctgccatttc ccgttgcatc agcgtcatta
    14461 attcaccgtt gttatcggtg tagatatgtg ctggaaacag cgtcgcaccg gctttatcac
    14521 cacaaaagct gacgttcata atcaactgtt cgcggatgtt gagtgcaaat gacgtttcca
    14581 gccacagaat gccgccgtta tgaaattcaa tggtgccaaa cagcgaatct tcgacgctgt
    14641 aagttgccgg atcccactcg ccaaattgac cacagctctt ttgcgtgccg atcttttgaa
    14701 aactatgcgc attcacgctt ttcaccgccg gaaagcccag cacatacatc gcagcatcca
    14761 gcatatgaat gccgatgtcg atcagcaggc caccaccctg cagttcttta ttggtaaaga
    14821 caccccagcc gggaacgccg cagcgacgca gggcgcgggc ggtggtaacg taaatctctc
    14881 ccaaaacgcc gttggtcacc tgttcacgca gctgttgcgt atcgagcgca aacgatggtg
    14941 aaagtcgtag gccagcacct tgcccagttt gcgcgcggta tcgcacattt cccacgcctg
    15001 ttctgtcgtc atggcgggcg gtttttcgca catcacatgg cagcctgctt ccagtgccat
    15061 cagggtatgt tcgtaatgaa aacggttagg tgagccgacg ctaaccacat caggtttcac
    15121 cgccagcagc atggcctgcg gatcgtccca cacggatgca ttcccgtatt tttctgccag
    15181 cgcctgcgcc tgggaaaggc ggctgtcaca gacagccacc agttccagat cgttgcgggt
    15241 gcagtagtac gaagcatgaa ccttatccgc cacctggccc gcgcctatta tcgcgacccg
    15301 cagcggagag cttgtcattg cacttttcac aaagacctct tagcaggtac gcaaccaggc
    15361 caacgaatca cggtacgcct gggcaggatc ttctgcccgg atacgccctt catacaccac
    15421 ataaccctga tagttatcgg cgcgcagctg ttcaaacagc gcgtggaaat cgagggtgcc
    15481 actgcccggc tggtaacgat ggttatcggc aatatgcaca tgaccgagca agtcacggtt
    15541 gtcgtgcagc gcctgcgcca ggttatcttc ttcgatgttc atgtgataga aatcgccgat
    15601 aatctgcaca tgtttaagat tgttttcgac gatgtaacga cgagcatcgg cgagggtgtt
    15661 gatcatatga tcctgatagc ggtttaacgg ttcgagatac accacggttc cggtacgcgc
    15721 ggcgacctgt tccagtacgc gcagggaatc actcaccagt ttgcggtcgc cgtccaggct
    15781 acgcggcgag gtcatcggcg gtaagcggaa ggtaaacatg ccccacgctg ccggaacccc
    15841 cccacttctg ccagcgcctc gagaatgcgt tcgatctgct ttaagccatt aagacgacgc
    15901 tcttcgataa agtcaccaat ccagccgtca tagccgccgc aggcggtggt cactggtaaa
    15961 ccggtttctt tgatcgccgc tttgacttct tcgatgttgt tgaccagcag tttgccgtca
    16021 atctcaaaac catcgaagcc catctctttg atataacgaa atctctcgcg gatattttcc
    16081 ggaaagaatg cctgattctg tgtgccgatt ttcatttttt cattcctttg aattaaaacg
    16141 taacgcccat tttgatgctc tgttccggat gctgatcgac atactgcatg tagctttccg
    16201 ggctgttggc aaaggtcact accgggtcga ttaaatcttc gcaattgaga taaccgttca
    16261 tcagcagttc ccagcaggtt tcttcaatac gcttacggct ccagcgcgga taatccgggt
    16321 tcggttcgct gcacgcgcgg gagaaaacaa ttttggcgtt attgaaatgc gcttcgcgtc
    16381 cgaggttaaa accttcagca aatggtttgg caaacgcgac ataggagatg gtgccgccgt
    16441 aggccaggcc gcgcagcgcc gattgcagcg cgtcggcgta accgctggtt tcgataatca
    16501 catcagcccc ctgcttgccg gtcagcgttt tgatctcttt gcctacgtca gtgccaatgg
    16561 ggttaaggca gaaatcagca ccgtggcgac gggcaatatc acagcgatgg gcgataggat
    16621 cgacgccaat taccaccgat gcgccagcgc gtttcgccag ttggatggca atttgaccga
    16681 tcgcgccaag ccctaccacc acgacaaaat cccctacgcg tacgttggca tcgcgcacgc
    16741 cgctcatggc aaactgcgcc gggtcgtaac agacggcgtt tttccaggag ctgccttgcg
    16801 gcattttgcg cagcttgtag ttattcactg cgttaatgat gaccgtctcg gagagcgggc
    16861 cgtagccgca tacgctgtcg cccaccgcgt agtctgtaac gtcgctgccg cactcgataa
    16921 tgtcgccaac caccatgttg ccaagctgga atttgccaaa ctcaatgccg cgcggcgcat
    16981 ctgccggacg cggggtgaac atctgccact cgccgttaaa gtcttcatcg ataaacgggc
    17041 tggcggcgcg gaagtcgacc acttccgttc cgtgtttcgg tgcgccgaaa cgcacgcgga
    17101 tcttcacttc attggctaaa atcgcccgat cttcatactc aaccagcgca gcaacacgcg
    17161 gtgctgtggc tactaacttt ttcatgtaaa actccttgta aagaaactgg ccgctttagc
    17221 ccttcacgcc accggcggtc aaaccactct taataaaacg ttccaaaagc gcatacataa
    17281 tcaccaccgg caatgcggtc accagcgagg ccgccatcat ccgtccccag atgtaatcag
    17341 gcgtactgaa cagcgcgttc aggcccactg gtaaggtgaa attgctggcg ctggagagga
    17401 aaatcgacgc aaacagatag tcgttccacg ccaccataaa gcagtagacg aacaccgata
    17461 tcagcccgga catcgccagg ggcacggtga tacggaagat gatttgcaga cggttgaggc
    17521 catccatcat cgccgcttct tcgatctcat cagggatggt gtcgaagtaa cttttcagca
    17581 taaacacggc ggtgggcagg gtttgcgtca ccatggtaat gatcagcgcc atctcggtgt
    17641 cgtaaatacc aagcgcggtg atgattttga acagaggcac caccagcaaa atgccagaga
    17701 acatatacac cgtgtaaaag ctggcgttga tggtcatccg gcctttaaag cgcaggcggg
    17761 aaagcgcgta agctccgaga atgccgagaa ataccgccac cacggaggaa accaccgaca
    17821 ccaccatgct gttacggaag tagtcgacaa acgggaaaat catcgggtta aaaatgtcga
    17881 cgtaatgctc cagcgtccac tgttgcggca gcagcgtagg atgcagcgat atcgcctctt
    17941 tcgcgctttt gaacgaggtc atcagcatca caaaaaacgg aaacagcgtg atgatgagaa
    18001 acagcgccag cccgcagtaa aaaccgatac gactgagcgt gcgtttattt gttgccattg
    18061 aggttcaccc gtttcctggt cagcagaatg acagcgaaaa taatgatgaa gagcaccacc
    18121 gagatcgccg ccgctttccc taagtcatta aaggcaaagg cggttttgta gagatagaca
    18181 ccaagaatat cgaccttggt tgtcagcaga taaacatcag cgaacatgta gaacatccag
    18241 atggtgcgca gggtcaccac cgtcgccagg accggcataa ttgcgggcag cgtgacgatg
    18301 cgaaaccgct gccaggcatt agcgccatcc atctctgccg cttcgtacag cgatttgtcg
    18361 atggtctgca aaatcgccag aaacgagata aaggcatacg ggaagtagcg ccagatggcg
    18421 aacagcacca ccagcacaaa gctactgccc ggattatcaa accacagcgg tgcctgctca
    18481 tagagatgca gaagatcgac gccgaggtag ttcacaatgc cgtagccgtt gttgaacatg
    18541 tatttccagg caaacaccag cgaaatggac ggcgttacgt aggagaggat caccagcgaa
    18601 cgcgcggttt tgcgcaggcg gaactcgcgg ttgaaaaaca tcgccacggc cagcccgagc
    18661 acggtgctgc ccgccaccac cagcgccgta taccagaccg tcatccacag cgagtgccag
    18721 aagccgggat cggagagaat gcgcacatag ttactcaccc caacaaacgt tgactcgatg
    18781 ttgggattga gcggcagacg taaaaaactg atttcgatat tcgacaccat cggccacgcc
    18841 accagaccgc ccagcagtaa taagctgggc gcgagaagca gcagcgcaaa cggcatatcg
    18901 gaacgacctg aaaaaagcct gttcattgcg gattcctgtc ctgggtttag tgctgttcga
    18961 tcagttcatc cagctttttc tggctcgctt gcagcgtgcc agggagatcg gctttaccca
    19021 cggtgacgtt atgcaccatg gagctcacca caccagaacc cgtcacatcg cccatgcggg
    19081 taaagttttt atcccctact gcgccaaaga cctgaatatt tggcagttca ccgattagct
    19141 gattcggtag ttcacccagc gccttaataa cgtcgttgtc tttccaggtg gcggtagtga
    19201 caaccgcttt attcaccggc agcgcagcac ctggcgacat catcacccag tcggcaatgt
    19261 tgtccgcctg ctccataaag gtgacaaatt tttctgctgc ttcggtcttt tgtccggcgg
    19321 taatggtcag cgaggtcaac atgccgtaga ccgcagagtt tttctcggtt ggcaccacga
    19381 aaccgacgtt tttcgggtcg ccttctttaa tcacagccgg aaggatatag gtggagtaaa
    19441 tcgccatcgg cgcggtgccg ttcataaagg cgtctttcac ttccatgatg tcgttagaac
    19501 ccggcatggt gttggcagca aggtcgcggt aataggtcag cgcctgcatc atctctggtg
    19561 tatcaagggt gattttgcct tcggcgttaa agacgttggc ctagttggat aacgcaaact
    19621 gggagaagga ttgttccgtc agcacgcttt ctgccgtagg cagcgcaata ccgtattttt
    19681 tattcgccgg gtcattcagt ttctgtgcaa cgtccagcag ctgttgccag tttttcggct
    19741 cctccagccc cgcttttgcc agcacatctt tgcgatacca gatgccgcca atccaggcgc
    19801 tgacaggaac accggtccat gcgctaccat cttcggtacg cacaatacgc agtacgccat
    19861 cgtaaaacgc gccttcacca acgttgctga tgaccgtggc aaccgctttg cgatcgataa
    19921 gctgctcttt atccataact ttggcgtagt catggctggt ttcgatcact tccggcagcg
    19981 agccgctacg tgaaagggtg atgactttgg tgttatgagc gtcttcttcc acgggcactt
    20041 gtttaacggt gatacctggg ttttcttttt caaaacgggc aatcaatttg ctgataactg
    20101 cctgacgctc ctgctccacc gaagaatgca taaattcgat ggatacatgg gttttatttt
    20161 cttctttaca gcctgaaatc agggcgcagg aaaccagtgc tgataacagc acgatttttg
    20221 atttaatcat gagatgttcc tttttattta atccacataa cctgccaggg gcgcagcgtt
    20281 atttcattac ccaatatatc aacttcacta atcagatccc gaccatgcag atcagtaata
    20341 tttacatgct gaatattttt actgacatta aacaatccag tcagacaatt accatcagcg
    20401 ttacttcttt gaatacgcat tacggatgaa ttaaccgtat cgatggtaaa attattatcc
    20461 ggatgaaact cgttatggct gcgacgaagt gtaattaaac gcgacaactc atgatatacc
    20521 gcatgcctta atgtagcttc atcgttcagt tctcgtgtta tctcttcact gtgatatttt
    20581 ttacggttaa tcgcacggtt atatccgagt ttttcgacac ctgcgtaatc attacgcgag
    20641 ccaagaatac tttgaatata tatcgcgggt acaccgggga aacttaacaa aatcgcatgg
    20701 gcaaggataa acctggtgca acgttcttca tcgctactct cgcggcggct taacgcattc
    20761 atataggtga cattcatctc atagggactg cgcgtgccgt cgggattatt ttt
//
LOCUS       DAJTOW010000068        20698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTOW010000068 DAJTOW010000000
VERSION     DAJTOW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.068"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            81..350
                     /locus_tag="OO244_002626"
     CDS             81..350
                     /locus_tag="OO244_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5664394.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OO244_002627"
     CDS             344..709
                     /locus_tag="OO244_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5664395.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO244_002628"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO244_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5664396.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OO244_002629"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OO244_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5664397.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OO244_002630"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OO244_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5664398.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OO244_002631"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OO244_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5664399.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO244_002632"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO244_002632"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5664400.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO244_002633"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO244_002633"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5664401.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="OO244_002634"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="OO244_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO244_002635"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO244_002635"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5664402.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO244_002636"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO244_002636"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5664403.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="OO244_002637"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="OO244_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5664404.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="OO244_002638"
     CDS             complement(16437..17195)
                     /locus_tag="OO244_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5664405.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="OO244_002639"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="OO244_002639"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5664406.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="OO244_002640"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="OO244_002640"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5664407.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="OO244_002641"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="OO244_002641"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5664408.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="OO244_002642"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="OO244_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4892 a         5695 c         5215 g         4896 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgcat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccaga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgccag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatatccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggaa attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttg
//
LOCUS       DAJTOW010000069        20613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTOW010000069 DAJTOW010000000
VERSION     DAJTOW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.069"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            97..396
                     /locus_tag="OO244_002643"
     CDS             97..396
                     /locus_tag="OO244_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5664409.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="OO244_002644"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="OO244_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5664410.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO244_002645"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO244_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5664411.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="OO244_002646"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="OO244_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5664412.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="OO244_002647"
     CDS             complement(2535..2879)
                     /locus_tag="OO244_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5664413.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHSTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="OO244_002648"
     CDS             3031..3261
                     /locus_tag="OO244_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664414.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="OO244_002649"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="OO244_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5664415.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="OO244_002650"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="OO244_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5664416.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="OO244_002651"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="OO244_002651"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5664417.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="OO244_002652"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="OO244_002652"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5664418.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="OO244_002653"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="OO244_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5664419.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="OO244_002654"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="OO244_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5664420.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="OO244_002655"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="OO244_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5664421.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="OO244_002656"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="OO244_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO244_002657"
     CDS             12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO244_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5664422.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="OO244_002658"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="OO244_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5664423.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="OO244_002659"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="OO244_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5664424.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="OO244_002660"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="OO244_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="OO244_002661"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="OO244_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5664425.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO244_002662"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO244_002662"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5664426.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO244_002663"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO244_002663"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5664427.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19828)
                     /locus_tag="OO244_002664"
     CDS             complement(19400..19828)
                     /locus_tag="OO244_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5664428.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO244_002665"
     CDS             complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO244_002665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5664429.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4973 a         5276 c         5147 g         5217 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcacga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatatta gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tgaatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtggttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaaggaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgactat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaacccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    19621 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    19681 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    19741 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    19801 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    19861 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg
    19921 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    19981 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    20041 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact
    20101 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    20161 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    20221 tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta
    20281 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc
    20341 caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    20401 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    20461 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    20521 gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    20581 tagccgagta ctctatccag ctgagctacg gat
//
LOCUS       DAJTOW010000070        20542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTOW010000070 DAJTOW010000000
VERSION     DAJTOW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.070"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO244_002666"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO244_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5664430.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO244_002667"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO244_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5664431.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO244_002668"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO244_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5664432.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO244_002669"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO244_002669"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5664433.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO244_002670"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO244_002670"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5664434.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO244_002671"
     CDS             4449..4814
                     /locus_tag="OO244_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5664435.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO244_002672"
     CDS             4964..5482
                     /locus_tag="OO244_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5664436.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO244_002673"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO244_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO244_002674"
     CDS             6713..7195
                     /locus_tag="OO244_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5664437.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO244_002675"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO244_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5664438.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO244_002676"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO244_002676"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5664439.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO244_002677"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO244_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5664440.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO244_002678"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO244_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5664441.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO244_002679"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO244_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5664442.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO244_002680"
     CDS             10939..11730
                     /locus_tag="OO244_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5664443.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO244_002681"
     CDS             11751..13037
                     /locus_tag="OO244_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5664444.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO244_002682"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO244_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5664445.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO244_002683"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO244_002683"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5664446.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO244_002684"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO244_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5664447.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO244_002685"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO244_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5664448.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO244_002686"
     CDS             complement(16985..17275)
                     /locus_tag="OO244_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5664449.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO244_002687"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO244_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5664450.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO244_002688"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO244_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5664451.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO244_002689"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO244_002689"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO244_002690"
     CDS             19137..20378
                     /locus_tag="OO244_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5664452.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGTKGMKLVG"
BASE COUNT         5308 a         5365 c         4817 g         5052 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgcgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgcagaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gacaaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgagtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ct
//
LOCUS       DAJTOW010000071        20494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTOW010000071 DAJTOW010000000
VERSION     DAJTOW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.071"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO244_002691"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO244_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5664453.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO244_002692"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO244_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5664454.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO244_002693"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO244_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5664455.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO244_002694"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO244_002694"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5664456.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OO244_002695"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OO244_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5664457.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OO244_002696"
     CDS             complement(8218..9111)
                     /locus_tag="OO244_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5664458.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO244_002697"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO244_002697"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5664459.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO244_002698"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO244_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5664460.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OO244_002699"
     CDS             complement(10242..11474)
                     /locus_tag="OO244_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5664461.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKTRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO244_002700"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO244_002700"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5664462.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO244_002701"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO244_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5664463.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="OO244_002702"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="OO244_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO244_002703"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO244_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5664464.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO244_002704"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO244_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5664465.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO244_002705"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO244_002705"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5664466.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..>20494)
                     /gene="ycaL"
                     /locus_tag="OO244_002706"
     CDS             complement(19829..>20494)
                     /gene="ycaL"
                     /locus_tag="OO244_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5664467.1"
                     /translation="SGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRL
                     AKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHE
                     LGHVALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKE
                     SEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPSTERAQHIRDRIA
                     SGK"
BASE COUNT         4909 a         5681 c         4992 g         4912 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 gttttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cgga
//
LOCUS       DAJTOW010000072        20357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTOW010000072 DAJTOW010000000
VERSION     DAJTOW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.072"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..447)
                     /locus_tag="OO244_002707"
     CDS             complement(<1..447)
                     /locus_tag="OO244_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5664468.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO244_002708"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO244_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5664469.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO244_002709"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO244_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5664470.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO244_002710"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO244_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5664471.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="OO244_002711"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="OO244_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5664472.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="OO244_002712"
     CDS             complement(5217..6815)
                     /locus_tag="OO244_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5664473.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO244_002713"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO244_002713"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5664474.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="OO244_002714"
     CDS             complement(8494..9072)
                     /locus_tag="OO244_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5664475.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO244_002715"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO244_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5664476.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="OO244_002716"
     CDS             10511..10690
                     /locus_tag="OO244_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5664477.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10810..11169)
                     /locus_tag="OO244_002717"
     CDS             complement(10810..11169)
                     /locus_tag="OO244_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5664478.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11531..11875)
                     /locus_tag="OO244_002718"
     CDS             complement(11531..11875)
                     /locus_tag="OO244_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5664479.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12007..13917
                     /locus_tag="OO244_002719"
     CDS             12007..13917
                     /locus_tag="OO244_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5664480.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13975..14670
                     /gene="tsaB"
                     /locus_tag="OO244_002720"
     CDS             13975..14670
                     /gene="tsaB"
                     /locus_tag="OO244_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5664481.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14710..15291
                     /gene="yeaY"
                     /locus_tag="OO244_002721"
     CDS             14710..15291
                     /gene="yeaY"
                     /locus_tag="OO244_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5664482.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15497..17182
                     /gene="fadD"
                     /locus_tag="OO244_002722"
     CDS             15497..17182
                     /gene="fadD"
                     /locus_tag="OO244_002722"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5664483.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17264..18379
                     /gene="rnd"
                     /locus_tag="OO244_002723"
     CDS             17264..18379
                     /gene="rnd"
                     /locus_tag="OO244_002723"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5664484.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18433..19398)
                     /gene="yeaX"
                     /locus_tag="OO244_002724"
     CDS             complement(18433..19398)
                     /gene="yeaX"
                     /locus_tag="OO244_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5664485.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19454..20071)
                     /gene="yeaW"
                     /locus_tag="OO244_002725"
                     /pseudo
     CDS             complement(19454..20071)
                     /gene="yeaW"
                     /locus_tag="OO244_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         5021 a         5091 c         5276 g         4969 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacgacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat
      901 gaagaacagc agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgcgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tatcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatctt tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg ttcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat
    10801 aaatttaact cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg
    10861 atttttaatc acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc
    10921 cgtgaaccca gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc
    10981 gggtactccc agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga
    11041 acttggtgtt ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac
    11101 agcaggcata ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt
    11161 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
    11221 caggtgtttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
    11281 gtcagcccgc acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc
    11341 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat
    11401 tgaaattata aatataaata atgaattagc agaagcatca acagtaatat cttattctct
    11461 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
    11521 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
    11581 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
    11641 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
    11701 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
    11761 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
    11821 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
    11881 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc
    11941 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
    12001 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt
    12061 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
    12121 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
    12181 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
    12241 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
    12301 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
    12361 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
    12421 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
    12481 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
    12541 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
    12601 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa
    12661 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
    12721 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
    12781 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
    12841 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
    12901 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt
    12961 tatgatgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
    13021 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga ttaatcagcc gggctacagc
    13081 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
    13141 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
    13201 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
    13261 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
    13321 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
    13381 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
    13441 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
    13501 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
    13561 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac
    13621 aaattgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
    13681 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
    13741 cagggggtag gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
    13801 tatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
    13861 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
    13921 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
    13981 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
    14041 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
    14101 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
    14161 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg
    14221 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
    14281 tggcgcaaaa acggcgcaac acgcgtgctg tcagccattg atgcgcgaat gggcgaagtt
    14341 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
    14401 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg
    14461 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
    14521 gatggcgaag tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt
    14581 gccgagggta aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc
    14641 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
    14701 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
    14761 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag taccacgccg
    14821 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
    14881 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
    14941 accgtaccgc tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt
    15001 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
    15061 gtcgggccaa tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg
    15121 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
    15181 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
    15241 ggctggtata atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt
    15301 tttaaagaat gcagaaacag cggctggtcc gctgtttctg cattcttacg gtaaagataa
    15361 aaataaatag tgacgcgctt cgcaaccttt tcgttgggta attatcaagc tggtatgatg
    15421 agttaatatt atgttaacgg cgtgtatatc atttggggtt gcgatgacga cgaacacgca
    15481 ttttagaggt gaagaattga agaaggtttg gcttaaccgt tatcccgcgg acgttccgac
    15541 ggagatcaac cctgaccgtt atcaatctct ggtagatatg tttgagcagt cggtcgcgcg
    15601 ctacgccgat cagcctgcgt ttgtgaatat gggggaggtg atgaccttcc gcaagctgga
    15661 agaacgcagt cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga agaaaggcga
    15721 tcgcgttgcg ttgatgatgc ctaacttatt gcaatatccg gtggcgctat ttggcatttt
    15781 gcgtgccggg ataatcgtcg taaacgttaa cccgttgtat accccgcgtg agcttgagca
    15841 tcagcttaac gatagcggcg catcggcgat tgttatcgtg tctaactttg cccacactct
    15901 ggaaaaagtg gttgataaaa ccgccgttca gcacgtaatt ctgacccgta tgggcgatca
    15961 gctatctacg gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca agcgcttggt
    16021 gccgaaatac catctgccag atgccatttc atttcgtagc gcactgcata acggctaccg
    16081 gatgcagtac gtcaaacccg aactggtgcc ggaagattta gccttcctgc aatacaccgg
    16141 cggcaccaca ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc tggcgaacct
    16201 ggaacaggtc aacgccacct atgggccgtt gctgcatcca ggtaaagaac tggtagtaac
    16261 ggcactgccg ctgtatcaca tttttgccct gaccattaac tgcctgctgt ttatcgaact
    16321 gggtgggcag aacctgctta tcactaaccc gcgtgatatt ccagggttgg taaaagagtt
    16381 agcgaaatat ccgtttaccg ctatcactgg cgttaacacc ttgttcaatg cgttgctgaa
    16441 caataaagag ttccagcagc tggatttctc cagtctgcat ctttccgcag gcggtgggat
    16501 gcctgttcag caagtggtgg cagaacgctg ggtgaaactg accggacagt atctgctgga
    16561 aggttatggc ctgaccgagt gcgcgccgct ggtgagcgtt aacccgtatg atattgatta
    16621 tcatagcggt agcatcggtt tgccggtgcc atcgacggaa gccaaactgg tggatgacga
    16681 cgacaacgaa gtaccaccgg gtcaaccggg tgagctttgt gtcaaaggac cgcaggtgat
    16741 gctgggttac tggcagcgtc cggatgctac agatgagatc atcaaaaatg gctggttaca
    16801 caccggcgac atcgcggtga tggatgaaga aggattcctg cgcattgtcg atcgtaaaaa
    16861 agacatgatt ttggtttccg gttttaacgt ctatcccaac gagattgaag atgtcgtcat
    16921 gcagcatcct ggcgtacagg aagtcgcggc tgttggcgta ccttccggct ccagtggtga
    16981 agcggtgaaa atcttcgtag tgaaaaaaga tccatcgctt accgaagagt cactggtgac
    17041 tttttgccgc cgtcagctca cgggatacaa agtaccgaag ctggtggagt ttcgtgatga
    17101 gttaccgaaa tctaacgtcg gaaaaatttt gcgacgagaa ttacgtgacg aagcgcgcgg
    17161 caaagtggac aataaagcct gagcgttaag tcagtcgtca gacgccggtt aatccggcgt
    17221 tttttttgac gcccactaaa gagaaaacaa tttgaattac caaatgatta ccacggacga
    17281 tgcgctggct tctttgtgtg aagccgtccg tgtctttccg gcgatagccc tggatactga
    17341 atttgttcgt acgcgcactt attacccgca gctggggttg attcaacttt tcgatggcga
    17401 gcatctggcg ctaattgatc ctctcgggat caccgactgg tcaccgctga aagcgatcct
    17461 gcgcgatccg tccatcacaa aatttctcca tgcaggcagt gaagatctgg aagtgttcct
    17521 caatgtcttt ggcgaattac cacaaccctt gattgacacg caaatccttg ctgccttctg
    17581 cggacgcccg atgtcatggg gtttcgcttc catggtggaa gagtattccg gcgttacgct
    17641 ggacaagagt gaatcgcgca ccgactggct ggccagaccg ctgaccgaac gtcagtgtga
    17701 atacgcagcg gcggatgtct ggtatctgtt accgatcacc gccaagctta tggtagaaac
    17761 ggaggcctcc ggctggctac ctgcggcgct ggatgaatgc cgcctgatgc aaatgcgtcg
    17821 tcaggaagtc gttgcgccgg aagatgcctg gcgtgatatc accaatgcct ggcagttacg
    17881 cacacgccaa ctggcctgtc tgcaactgtt agccgactgg cgactgcgca aggcgcgaga
    17941 gcgtgatctg gcggtgaact ttgtcgtgcg tgaagagcat ttgtggtcgg tcgcgcgtta
    18001 tatgccggga agtttaggcg aactggacag cctgggttta tccggtagcg aaatccgctt
    18061 tcacggtaaa acgctgctgg cgctggtgga aaaagcgcag acattgccgg aagatgcctt
    18121 accgcagccg atgcttaacc tgatggacat gcctggttat cgtaaagcgt ttaaagcgat
    18181 taagtcgctg attactgacg tgagcgaaac gcataagatc agcgccgaat tgctggcatc
    18241 gcgtcggcaa atcaaccaac tgctgaactg gcactggaaa ctgaaaccgc agaacaattt
    18301 gccggagctg atttccggct ggcgtggtga gctgatggcg gaagcattac acaatttatt
    18361 gcaggaatat ccgcagtaaa atcttccgaa gccggactgg gcgcgctcag cgccacatcc
    18421 ggctttggca aactacaaat ccaacaccag gcgtttaccc ttcgcacgcg aacaacagat
    18481 caacatactt tgctggctgg cacgctcttc atcgctaaaa tactgatccc gatggtcagc
    18541 ttcaccttcc agtattgccg tttcgcaggt tccgcatacc ccttcacgac ataaacattc
    18601 cactttcgcg gctttattat tttcaataac ctgcaaaata gtcatctctt ccggcaccac
    18661 aaactctttt ccggaacggg caagcaccag ggtaaatgca tcgccggttt tgtcttcgat
    18721 agcaaattgc tcaaagtgca gtgtatcggc ggcgatgtcc agacgcgcag cttcacttct
    18781 taccgcttca attagcgcct cggggccaca ggtataaacg tgtgtgccag gttcgatatc
    18841 cgccaataat cgcgctaatt ccagtcgtgt tccggttgat gaatgatgca aatggacttt
    18901 ctcagcctgc ggatgctgga ctagctcagc acgaaatgcg caactttctg gatttcgcga
    18961 gcagtaatgt agctgccagt cgatgtcgct gtgttgcaac tctgccatgt gcgacaggaa
    19021 aggggtgata ccaataccgc ccgcgataaa cagatgcttt ctggctgagg gaattagcgc
    19081 aaacaggtta ttaggcgttg agatcgttaa ccgatcgccc acttttacct gctgatgcaa
    19141 aaagcgggaa ccgccgcgcg agttttcttc cagtcgaacg gcaatctgat aactagaggt
    19201 gttatgcggt gaactcagta gtgaatacgc attgctgtac tggttatcac catcgctcat
    19261 ctgcactagg atgtgacttc ctccggtgaa cgcaggcagc ggtttgccat cggttgccac
    19321 cagcgtgaag cgtttcacct gttcggtaag gggttcaacc tggctcacct gtacttcaaa
    19381 catttgatag tctgacattg ctgtactcca tcaaaaatca gcccagcggc ggaaaatacc
    19441 gccgccgatg tcattagtcc ttaaacacct gcgccagcag attatggaaa tgggcgatac
    19501 catgttcgga aatgccacta ccgctactgt cggccatgat gcgcccctga ccacgatagc
    19561 cacgcgattt cagccctttc tgtacgcttt caaccagacg taaatcttcc ggatggaaca
    19621 catcgcgata ccactcaatc agcgattttt gctcgtcggt taactcttca ttggtgaagt
    19681 aaatatcgta gttttgcagg gtagtttcag aatccaccgg gaattcataa atgaccgtca
    19741 tcatcccttt gatcggggtg acgttcagca tcgtgcacgg ccacagccag aaaccgtgga
    19801 atgccgcatc cgtaccctct tcaaatttaa acgactgttc ggacggtttg gcgaaaccgt
    19861 attgcagcgt ccagttaccg tgcatggtgt gccagtaacg atcaacctgt acggagtcgg
    19921 agaaacctag atgcgccgga ccacagtgat agcactcgag atagttatcg acaatgttct
    19981 tccagttggc aggcgtgcgg gtggtaaagc gggccgctag tttcagattg tggacttccg
    20041 ggcaggcttc cagcactttc gcgcccagac cggtgtattg attcacttga agtacgaaaa
    20101 aaaaccggga ggacattgga ttattcgggt tctgatggga ttagatttgg tggggcttgc
    20161 aagcctgtag cgcaaatttt agtcgttaat caatgaaacg cgaaagatag taaaaaattg
    20221 cttttgtttc attgaaaata cgaaaaacaa aaacactgca aatcatttca ataacagctt
    20281 caaaaaacgt tcaaaaccga taacaaccag gctgtaacca aatgactcat atcacaaatc
    20341 agctcatgcc gtttagg
//
LOCUS       DAJTOW010000073        20277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTOW010000073 DAJTOW010000000
VERSION     DAJTOW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.073"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO244_002726"
     CDS             complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO244_002726"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5664486.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO244_002727"
     CDS             complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO244_002727"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5664487.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1718..2989)
                     /locus_tag="OO244_002728"
     CDS             complement(1718..2989)
                     /locus_tag="OO244_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664488.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFDERKFSTRLNTIKHRG"
     gene            complement(3172..4377)
                     /locus_tag="OO244_002729"
     CDS             complement(3172..4377)
                     /locus_tag="OO244_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664489.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4477..4833)
                     /locus_tag="OO244_002730"
     CDS             complement(4477..4833)
                     /locus_tag="OO244_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5664490.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4969..5076)
                     /gene="rprA"
                     /locus_tag="OO244_002731"
     ncRNA           complement(4969..5076)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO244_002731"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5262..6374)
                     /gene="ydiK"
                     /locus_tag="OO244_002732"
     CDS             complement(5262..6374)
                     /gene="ydiK"
                     /locus_tag="OO244_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5664491.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6763..9819
                     /locus_tag="OO244_002733"
     CDS             6763..9819
                     /locus_tag="OO244_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5664492.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9816..10226
                     /gene="menI"
                     /locus_tag="OO244_002734"
     CDS             9816..10226
                     /gene="menI"
                     /locus_tag="OO244_002734"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5664493.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10326..10514
                     /locus_tag="OO244_002735"
     CDS             10326..10514
                     /locus_tag="OO244_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5664494.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11062..11430
                     /gene="sufA"
                     /locus_tag="OO244_002736"
     CDS             11062..11430
                     /gene="sufA"
                     /locus_tag="OO244_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5664495.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11439..12926
                     /gene="sufB"
                     /locus_tag="OO244_002737"
     CDS             11439..12926
                     /gene="sufB"
                     /locus_tag="OO244_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5664496.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12936..13682
                     /gene="sufC"
                     /locus_tag="OO244_002738"
     CDS             12936..13682
                     /gene="sufC"
                     /locus_tag="OO244_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5664497.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13657..14928
                     /gene="sufD"
                     /locus_tag="OO244_002739"
     CDS             13657..14928
                     /gene="sufD"
                     /locus_tag="OO244_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5664498.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14925..16145
                     /gene="sufS"
                     /locus_tag="OO244_002740"
     CDS             14925..16145
                     /gene="sufS"
                     /locus_tag="OO244_002740"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5664499.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16158..16574
                     /gene="sufE"
                     /locus_tag="OO244_002741"
     CDS             16158..16574
                     /gene="sufE"
                     /locus_tag="OO244_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5664500.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16723..17727
                     /gene="ldtE"
                     /locus_tag="OO244_002742"
     CDS             16723..17727
                     /gene="ldtE"
                     /locus_tag="OO244_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5664501.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17790..18026)
                     /gene="lpp"
                     /locus_tag="OO244_002743"
     CDS             complement(17790..18026)
                     /gene="lpp"
                     /locus_tag="OO244_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5664502.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18337..19749)
                     /gene="pykF"
                     /locus_tag="OO244_002744"
     CDS             complement(18337..19749)
                     /gene="pykF"
                     /locus_tag="OO244_002744"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5664503.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5310 a         4857 c         5175 g         4935 t
ORIGIN      
        1 tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc ttatgcctga
       61 tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg cgcagaggct
      121 ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag acgagaaatt
      181 acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg ctcctgcatc
      241 tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc
      301 ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat ttcttcggct
      361 tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg cgcgtgggcg
      421 taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc gatcatatca
      481 accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt ggaaatcgcc
      541 tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc tggcatggtt
      601 tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc ggcaaagtgg
      661 tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc ttcggatttc
      721 acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc cgcaccaatg
      781 acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt
      841 caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt gccagtccat
      901 aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat cgttttgcaa
      961 ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac gcattcagtg
     1021 accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt ctcaaggggt
     1081 ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc ttcagcaaag
     1141 gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc gttaacccgc
     1201 tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag tttaatttct
     1261 tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac
     1321 agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg aatatggccc
     1381 gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt gttgatggca
     1441 cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag ttgtttgttc
     1501 ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat tgctccggga
     1561 aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc tttttctaag
     1621 gctttattct gcatttcagg cgataaactg tggcggatag gataagccat caatccaatc
     1681 aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt
     1741 ttaaccgggt gctaaatttg cgttcgtcaa accgcacatc attctcagga ataataaaaa
     1801 cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa
     1861 aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa
     1921 taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt
     1981 tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga
     2041 aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg
     2101 cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc cggaccattt
     2161 ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga
     2221 tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc
     2281 acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac
     2341 cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct acgctggcgt
     2401 ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt acaaacagaa
     2461 tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata tagctcacca
     2521 gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg accgccgaac
     2581 cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc agcgctgagt
     2641 tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg caagcgggaa
     2701 taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc cgacgaccaa
     2761 acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa ccgataccgg
     2821 aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga gatgtcatat
     2881 tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa atacaaagaa
     2941 cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt gttctccgac
     3001 ggtttacata attactatac tgaagatgat tttccctggg tacatggcat aaagtttatg
     3061 tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt agttagcgca
     3121 gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc attacccacc
     3181 cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat
     3241 aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg caatacttct
     3301 ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc ccgtactgta
     3361 atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta acgtcaggcc
     3421 aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata ttatcaccac
     3481 ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa aggtgtacag
     3541 catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca
     3601 aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt atgacatgcc
     3661 tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg ttgccattga
     3721 gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat gttccgtaga
     3781 gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga acgtacaacg
     3841 gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg accaaccgaa
     3901 ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt gtccgctgga
     3961 aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag cttccatcaa
     4021 actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac
     4081 ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca tatagcagca
     4141 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
     4201 caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac ctgcggcatt
     4261 agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
     4321 atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg
     4381 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta
     4441 ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct ccagatcgtg
     4501 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
     4561 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
     4621 gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac gcatagccaa
     4681 cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact gacgccagct
     4741 ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
     4801 atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
     4861 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
     4921 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
     4981 ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact caggggattt
     5041 ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg catacggact
     5101 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
     5161 ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa tatcaattag
     5221 cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cggtttctcg
     5281 atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg gacttcttcc
     5341 acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat aaacagaccg
     5401 atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat cagcggtaaa
     5461 tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc aaccacaccg
     5521 ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag ccagataatc
     5581 gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat cattaacacc
     5641 gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
     5701 tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg tgccgccagc
     5761 agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg aatgccttgt
     5821 gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag cgcacaatgc
     5881 accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata
     5941 taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt gtgccagcct
     6001 gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
     6061 atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
     6121 agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac cagaacggcg
     6181 agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
     6241 accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca cagacatgcc
     6301 acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc cctgggctga
     6361 cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
     6421 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggccgatt
     6481 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
     6541 gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt cgttcacccc
     6601 agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct gcaactcgaa
     6661 atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct gaaagaatag
     6721 tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc acagatttcc
     6781 ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga gcaacaaggt
     6841 tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac cgacaacagt
     6901 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg
     6961 atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc ccgcggcggc
     7021 ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat gtcccgccat
     7081 atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
     7141 ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc gccggaactt
     7201 tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc cggtcaggga
     7261 tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt gttgttgggg
     7321 ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct gggtaaatcc
     7381 aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
     7441 ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga tctgcgtcat
     7501 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
     7561 acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa agtgcgccgt
     7621 ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc gtttatggtt
     7681 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
     7741 gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa agagatgctc
     7801 ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga gcgagttaat
     7861 gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt gattggctgg
     7921 caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa aaaagccgtt
     7981 ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga ggatacctgc
     8041 gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct cgacagccac
     8101 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggca
     8161 ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga tgacgtggtg
     8221 gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg ttttcgcgct
     8281 gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg
     8341 gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga aggtctcgat
     8401 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca gatccccatt
     8461 gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac
     8521 tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg gattcattca
     8581 ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga tcgcggcgtt
     8641 gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
     8701 gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc acacgaagtc
     8761 aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg ccccatcaaa
     8821 attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg ttatttacgc
     8881 ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat ggcacgcgcg
     8941 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc ggaaaaacat
     9001 atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca aatggtgggg
     9061 catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga gcagaaagcg
     9121 cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca agtggtggcg
     9181 gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc attttcgcca
     9241 aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag
     9301 acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg cgtcgatccg
     9361 gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga acgtggcgag
     9421 tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca tccagtggct
     9481 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac cgctttgccg
     9541 ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc
     9601 agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccatgaaaat
     9661 tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc gcgtaaccgc
     9721 tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg cacgggggta
     9781 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg aaaatcaccc
     9841 tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat attcgctttg
     9901 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg acaaagcagc
     9961 ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc ggttccgttg
    10021 ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc aatgctaatc
    10081 acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg catctcggtt
    10141 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
    10201 cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt
    10261 ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
    10321 atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta cgccgtgacc
    10381 agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa cttgaatttg
    10441 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt gcctggcgtc
    10501 tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct ggattaacct
    10561 ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat tgttcaaaaa
    10621 taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt attcttatcg
    10681 ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg ggtagaaacc
    10741 cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg cactttttta
    10801 cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca gccttaatgc
    10861 tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt gcctgttaac
    10921 ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta cggtaaagcc
    10981 cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta tacgctgaaa
    11041 gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc acaggatttc
    11101 gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga gctggtggca
    11161 aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg cgcgggcttt
    11221 ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt tgaacacgac
    11281 ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac ggaagtcgat
    11341 ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc ccagaatgaa
    11401 tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat actgaagcaa
    11461 ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga ttcttcaccc
    11521 agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg
    11581 cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat cgcgcatggc
    11641 tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat tatcaggatt
    11701 acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg tctgaacctg
    11761 gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg
    11821 agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc attttcgact
    11881 ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt attttctgtt
    11941 cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc ggcaccgtgg
    12001 tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct gacggtacgt
    12061 ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat tttcgcatta
    12121 acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa gacagctacg
    12181 tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg cacgcggcag
    12241 tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta caaaactggt
    12301 tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg
    12361 aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg attacgtgga
    12421 aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac tcagtggcgc
    12481 tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc ggtaaaaaca
    12541 ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac agttatcgcg
    12601 gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag tgcgactcaa
    12661 tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca
    12721 gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa ctgttttact
    12781 gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac ggtttctgca
    12841 aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca
    12901 tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa gatttacacg
    12961 tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt cccggcgaag
    13021 ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca acgcttgccg
    13081 ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa gatttgcttg
    13141 cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag tatccggtgg
    13201 agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca gtgcgcagct
    13261 atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa gagaaaatcg
    13321 ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt ttttccggcg
    13381 gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag ttatgcattc
    13441 ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc gatggcgtga
    13501 actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa cgcattctcg
    13561 actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg aaatccggcg
    13621 atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc gaacagcagt
    13681 aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg ctcaccgcaa
    13741 gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa acatgaaaac
    13801 tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat tgcgggagat
    13861 atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg gctggtgttt
    13921 gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg atatgaagtg
    13981 agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt gtttctgcat
    14041 ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg
    14101 gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga ggtgaacact
    14161 gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt gatcgaacat
    14221 tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat caatgtcgca
    14281 gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag tcaccacttt
    14341 gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag tttcctgctg
    14401 ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa cagcacgctg
    14461 cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg tacctggctg
    14521 gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt cagcgacaaa
    14581 ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa aacggatggt
    14641 cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac aaaaccgcag
    14701 ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg gcgtattgat
    14761 gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg
    14821 atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg gcttaaacag
    14881 caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact ttttccgtcg
    14941 acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt
    15001 atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc gaggccgagt
    15061 tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc gcccaggcga
    15121 ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg
    15181 aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc aatagctggg
    15241 gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag caccacgcta
    15301 acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt gtgatccctc
    15361 tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat gagaaaactc
    15421 gcctgttggc aattactcat gtctccaacg tgcttggcac agaaaatcca ctggcggaaa
    15481 tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct caggcggtga
    15541 tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc tccgggcata
    15601 aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg ttgcaggaga
    15661 tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt gaaggcacta
    15721 cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg ggcatcattg
    15781 gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata gccgagtatg
    15841 aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat ctcactctct
    15901 atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac cacgcctatg
    15961 atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat cactgcgcta
    16021 tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg gccatgtata
    16081 acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac cgtctgctgg
    16141 gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg cgtaattttt
    16201 tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc cagcgtctgc
    16261 cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc cagagtcagg
    16321 tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc gacagcgatg
    16381 cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag atgacgccgc
    16441 aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc
    16501 tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc gccaaagccg
    16561 ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt gccggatggt
    16621 caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata agcaccatcc
    16681 gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg cgcgtctttg
    16741 cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc ggttgattat
    16801 ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa
    16861 ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat gttgatcctt
    16921 gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac tattccttca
    16981 caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc agagctgcgc
    17041 ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg tattggattg
    17101 caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc taacccaacc
    17161 tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa attaccgcca
    17221 gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct cgcgcatggt
    17281 aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt gcgcgtcagt
    17341 tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag cgtgcggacg
    17401 ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc taacgggatg
    17461 cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt tcagacaatg
    17521 ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt agatcagaag
    17581 ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag cagtggagca
    17641 actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga accagagcaa
    17701 gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat ggcgcacaat
    17761 gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc catgttgtcc
    17821 agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg
    17881 ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc agaagacagc
    17941 tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag gattaccgcg
    18001 cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt aatctccatg
    18061 tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg atgggaaggc
    18121 acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga taaaaaaatt
    18181 gagagaaaat aggatgcttc catcggattc atcttaaata aagtgagatt atatagtgaa
    18241 atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg
    18301 gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt
    18361 gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt cacctttgtg
    18421 tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt
    18481 gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt
    18541 ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc
    18601 agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt cagcagtttc
    18661 aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat tgttgaactc
    18721 gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc
    18781 ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt cagtaccgtc
    18841 gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat
    18901 ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat
    18961 catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg
    19021 cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg
    19081 gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg
    19141 gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttt
    19201 ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga
    19261 aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca
    19321 gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag
    19381 tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc
    19441 gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga
    19501 aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag
    19561 gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc
    19621 gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag
    19681 cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt
    19741 ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg
    19801 cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag
    19861 gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgtc
    19921 tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg
    19981 cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat
    20041 acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc
    20101 aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag gataagtgaa
    20161 agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat aatttatcgc
    20221 gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat atgctga
//
LOCUS       DAJTOW010000074        19748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTOW010000074 DAJTOW010000000
VERSION     DAJTOW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.074"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            270..1367
                     /gene="mltA"
                     /locus_tag="OO244_002745"
     CDS             270..1367
                     /gene="mltA"
                     /locus_tag="OO244_002745"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5664504.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1605..2411
                     /gene="tcdA"
                     /locus_tag="OO244_002746"
     CDS             1605..2411
                     /gene="tcdA"
                     /locus_tag="OO244_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5664505.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2462..2905)
                     /gene="csdE"
                     /locus_tag="OO244_002747"
     CDS             complement(2462..2905)
                     /gene="csdE"
                     /locus_tag="OO244_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5664506.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2905..4110)
                     /gene="csdA"
                     /locus_tag="OO244_002748"
     CDS             complement(2905..4110)
                     /gene="csdA"
                     /locus_tag="OO244_002748"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5664507.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4302..4529
                     /locus_tag="OO244_002749"
     CDS             4302..4529
                     /locus_tag="OO244_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5664508.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4880..5797
                     /gene="gcvA"
                     /locus_tag="OO244_002750"
     CDS             4880..5797
                     /gene="gcvA"
                     /locus_tag="OO244_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5664509.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5816..6211
                     /locus_tag="OO244_002751"
     CDS             5816..6211
                     /locus_tag="OO244_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5664510.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6204..7304
                     /gene="rlmM"
                     /locus_tag="OO244_002752"
     CDS             6204..7304
                     /gene="rlmM"
                     /locus_tag="OO244_002752"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5664511.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7348..8079)
                     /gene="fucR"
                     /locus_tag="OO244_002753"
     CDS             complement(7348..8079)
                     /gene="fucR"
                     /locus_tag="OO244_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5664512.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8137..8559)
                     /gene="fucU"
                     /locus_tag="OO244_002754"
     CDS             complement(8137..8559)
                     /gene="fucU"
                     /locus_tag="OO244_002754"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5664513.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8561..9980)
                     /gene="fucK"
                     /locus_tag="OO244_002755"
                     /pseudo
     CDS             complement(8561..9980)
                     /gene="fucK"
                     /locus_tag="OO244_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10089..11864)
                     /gene="fucI"
                     /locus_tag="OO244_002756"
     CDS             complement(10089..11864)
                     /gene="fucI"
                     /locus_tag="OO244_002756"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5664514.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11897..13213)
                     /gene="fucP"
                     /locus_tag="OO244_002757"
     CDS             complement(11897..13213)
                     /gene="fucP"
                     /locus_tag="OO244_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5664515.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFITVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13760..14407
                     /gene="fucA"
                     /locus_tag="OO244_002758"
     CDS             13760..14407
                     /gene="fucA"
                     /locus_tag="OO244_002758"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5664516.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14435..15583
                     /gene="fucO"
                     /locus_tag="OO244_002759"
     CDS             14435..15583
                     /gene="fucO"
                     /locus_tag="OO244_002759"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5664517.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15636..16391)
                     /gene="xni"
                     /locus_tag="OO244_002760"
     CDS             complement(15636..16391)
                     /gene="xni"
                     /locus_tag="OO244_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5664518.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16502..17869)
                     /gene="sdaB"
                     /locus_tag="OO244_002761"
     CDS             complement(16502..17869)
                     /gene="sdaB"
                     /locus_tag="OO244_002761"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5664519.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17927..19216)
                     /gene="sdaC"
                     /locus_tag="OO244_002762"
     CDS             complement(17927..19216)
                     /gene="sdaC"
                     /locus_tag="OO244_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5664520.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4871 a         4840 c         5210 g         4827 t
ORIGIN      
        1 atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc
       61 gtccgtggat gcgcactata ctccgctcgc gttttcatgc aacctttttt ttaccccgat
      121 cacggattta gcatgatttt gaacaaaaac aaaccactaa agcctatttt ttgtgcaaaa
      181 tttgcgtcag ggagaggctg ctcatttcat taaacgcggc ggtttgttat cttcgttgcg
      241 ccttattttt taacctgaag aagagaacaa tgaaaggacg ttgggtaaag taccttctta
      301 tgggcacggt tgtggcaatg cttgccgcct gctcttccaa accaaccgat cgcggacagc
      361 aatataaaga cgggaaattt acccagcctt tctctctggt gaaccagcca gatgccgttg
      421 gcgcgccgat taacgccggt gattttgccg agcaaattaa ccatatccgt aattcgtcac
      481 cgcgtctgta tggcaaccag agtaatgttt ataacgcggt gcaagagtgg ctgcgcgcag
      541 gcggtgatac ccgcaatatg cgccagttcg gcattgatgc ctggcagatg gaaggtgccg
      601 acaactatgg taacgtgcag tttacaggct attacacgcc ggtaattcag gcgcgccata
      661 cccgccaggg cgagttccag tatcctattt accgtatgcc gccaaaacgt ggtcgtctgc
      721 cgtctcgtgc ggagatctac gccggggcat tgggcgataa atacatcctc gcttacagca
      781 actctctgat ggataacttc attatggatg tgcagggtag tgggtatatc gactttggtg
      841 atggcagtcc gcttaacttt ttcagctatg cagggaaaaa cggtcatgcc tatcgcagca
      901 ttggtaaggt gctgatcgac cgtggcgaag tgaaaaaaga agatatgtcg atgcaggcga
      961 ttcgtcattg gggcgaaaca cacagtgaag ccgaggttcg cgagctgctg gaacagaacc
     1021 cgtctttcgt cttctttaaa ccgcaatctt ttgcaccggt gaaaggggca agtgcggtgc
     1081 cgctggttgg ccgtgcttct gttgcctccg atcgttcaat tattccgcca ggtactacct
     1141 tgctggcaga agtgccgctg ctggataata acggcaaatt taacggtcag tacgaattgc
     1201 gtctgatggt ggcgttggat gtcgggggcg caatcaaagg ccaacacttc gatatctatc
     1261 aagggatcgg gccggaagcc ggacaccgcg caggttggta caaccactat ggacgggttt
     1321 gggtgctgaa aacggctccg ggcgcaggta acgtctttag cggctgatgt gatattctgt
     1381 atgccgtttt tacggatatg acaggggagg ggtaacctca catcctgatt attgactaag
     1441 tgcgcgttgt tcataccgga tgcggcgtga acgccttatc cggcctacaa atcatgcaaa
     1501 tccaaaatat tgcaggagtt atgtaggcct gataagcgta gcgcatcagg catttatgcg
     1561 tttagggtga gggataacct cgcccttttt aattctgagg tgctatgtct gtggtaatta
     1621 gtgatgcatg gcgtcagcgt tttggtggca cagcgcgtct gtatggtgaa aaagcgttgc
     1681 aactgtttgc tgacgcgcat atttgtgtgg ttggtatcgg tggtgtcggt tcctgggcgg
     1741 cggaagcgct ggcgcgcacg gggattggcg caatcacgct tatcgatatg gatgatgtgt
     1801 gcgtcaccaa taccaatcgg caaattcacg ctctgcgcga taacgtcggg ctggcaaaag
     1861 cggaagtcat ggcggagcgc attcgccaga ttaacccgga gtgccgtgta acggtggtgg
     1921 atgatttcgt gacgccggat aacgtagcgc aatatatgag tgccggttat tcgtacgtga
     1981 ttgatgccat tgatagtgta cggcccaaag cggcgctgat tgcttattgt cggcgcaata
     2041 aaatcccgct ggtcacgacc ggtggcgcgg gtgggcagat tgacccgacg cagattcagg
     2101 ttaccgatct ggcgaaaacg attcaggatc cgctggcggc gaagttgcgc gagcgcctga
     2161 aaagcgattt tggcgtagtg aaaaacagta aaggtaagct cggcgtggat tgcgtgtttt
     2221 ctactgaagc gctggtgtac ccgcagtcag acggtacggt gtgtgcgatg aaagccacgg
     2281 cagaagggcc gaagcggatg gattgtgcat ctggatttgg cgcggcaacg atggtgaccg
     2341 ccacctttgg gtttgttgcg gtttctcatg cgctgaagaa gatgatggcg aaagcggcgc
     2401 gtcagggtta aacaatcagg tcggatgaga cgcgaacgtc ttatccgacc cggttctgtg
     2461 cttaaacctg cttcgtcgca gcgataatcg cctcgcttaa cgcatttaac ccctggctgc
     2521 gtgaggcgct aagctgcgca cgtaatccca gctcatcaaa caatgccagc ggcgactgtg
     2581 cctgtaattc ggcggcggtt ttcccctcaa cggcagtcaa caacaccgcc aacaggccgc
     2641 gcacaatgcg cccttcgctg ttgccaaaga agtgcatttt gccgttttca gccacggtat
     2701 atcccagcca gacgcggttt tcgcatccgg caatctcttt agcctgcgct tttaactcgt
     2761 ctggcaaagc cggaagctgt ttccccagca tgatcaactg gcgatattta tcttcccatt
     2821 gcgacaacgg tgcgaaggta ttgcgtaacg tttctgcggt tacggttgtg ccgaacggat
     2881 gtccggcgaa ttgcgggttt gtcattaatc caccaataat tccagcgcgc ggtcaacggc
     2941 attcaccagc gcatccacat cactctttgt attatatggc gcaaatgagg cgcgcagtgt
     3001 gccggttacg cctaattctg ccagtagcgg ctgagcgcaa tgttgcccgg cccgcaaggc
     3061 aataccgtac tccgccagta gcgtcaccat atcgctgtga tgaacgccag caaaatcaaa
     3121 ggccagcagg ctggaatcct ggcagcggaa tgaacgaaag cctggacgtt tcgccagcgc
     3181 ttcttctgca agcgttgcta agctacggct ccagctttcg gcctggttga tatcgtaatc
     3241 tgccagccat tccagcgccg cgcttaatcc tatgacgcca gcgacatttg gcgttcctgc
     3301 ttccagtttc cacggcgcag attgagtggt gaaaccgtca aaactcactt cgtgaatcat
     3361 tttgccgcca cccagccagg gcgacatcgc ttccagcagt tctgatttgc catacagcac
     3421 gccgataccc gtcgggccat acagtttgtg accagagaaa gcatagaaat cgatatccag
     3481 ttgctgaaca tccgcgggga aatgcaccgt cccctgagca ccatcaacca tcaccaccat
     3541 cccggctgaa tgagcaaagg taatcgctcg cgccagatcc gggcaaccgc cagtcacgtt
     3601 cgacatctga cccaacgcca gaatccgact acggggagta atcagctctg gcaacaaatc
     3661 gacatccggc agtcgctgcg cattaagcgg caatttcacc actttggctc tagtttgttg
     3721 ggcgaccatc agccagggga cgaggttagc gtggtgttct gccacgctga caataatctc
     3781 atcgcccggt tgcagacgcg gacgcgcata gcattgtgcc accatgttga tggattcagt
     3841 ggtgccgcgc gtccagacga tagttttatc atccggtgca ttcagtaatt gcgccacttt
     3901 ctctcgtgca gcttcataac gcgcggtcag gcgttgggct tcggcaaact ggctgcgatg
     3961 gacgtttccg gcgctcaggc tgtaaaactg tcgagtggct tcaaccacgg cttcaggttt
     4021 aagcgcggtc gcggcgctgt cgagatagac gcccgcatcc tgtagtgcgg gaaactgggc
     4081 gcgaaactgc gcgggattaa aaacgttcat ggtactcctc ggcttgatgc accgatcgtg
     4141 acgcaaaatt cactttgata caaggagagt gctaaccatc agaattatct ggatatcctg
     4201 gcgaagccgc gtaagtaaca tatgctgaat agtgttaggc gttaatttta gcctgttaat
     4261 caatactatt aagatttatt aatcgtttta aggagaagat gatgaaaaag actgccgcaa
     4321 ttatttctgc ctgtatgctg acttttgccc tgagcgcctg ttccggttcg aactatgtga
     4381 tgcacaccaa tgacggacgt accatcgtct ctgacggcaa accacagact gataacgata
     4441 tcggtatgat ttcgtataaa gacgctaatg gcaacaaaca gcagatcaac cgtactgacg
     4501 tgaaagagat ggtcgaactg gaacagtaat tcgcgatcgc aaggtaaaaa aaagcaccgc
     4561 aattaggcgg tgctacatta atcactatgg acagacaggg taaatgtaca ggaagtgaaa
     4621 aaaggtagct ttgctaccat ggtctgaatc gcagaccaat tgcaaacaca acaacacaac
     4681 atcacaaccg taagccaaaa gttcaccaga atacgcattc cgataaaact tttcgttccg
     4741 gctcaggaag tgccgccact ataggtattt gctggtagaa gctcaacgga caatttataa
     4801 tggctcagat taaaaaaact aataggttac acagtgtgat ctaattgtta aattcattta
     4861 acatcaaagt ttaaaagcca tgtctaaacg attaccaccg ctaaatgcct tacgagtttt
     4921 tgatgccgca gcacgccatt taagttttac tcgcgcagca gaagagcttt ttgtgaccca
     4981 ggccgcagta agtcatcaaa tcaagtctct tgaggatttt ttggggctaa aactgttccg
     5041 ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa agctatttcc tcgatatcaa
     5101 agagatattt tcgcaattaa ccgaagcgac gcgtaaactc caggcccgta gcgccaaggg
     5161 ggcgttgacg gtcagtttac tccccagttt cgccattcat tggttggttc cgcgactttc
     5221 cagctttaat tcagcttatc cgggaattga cgttcgaatc caggcggttg atcgtcagga
     5281 agataagctg gcggatgatg ttgatgtggc gatattttat ggtcggggca actggccggg
     5341 gctacgggtg gaaaaactgt acgccgaata tttattgccg gtgtgttcgc cgctactgct
     5401 gacaggcgaa aaacccttga agaccccgga agatctggct aaacatacgt tattacatga
     5461 tgcgtcacgc cgtgactggc agacatatac ccgacagttg gggtttaatc atatcaacgt
     5521 tcagcaaggg ccaattttta gtcatagcgc catggtgctg caagcggcta ttcacgggca
     5581 gggagtggcg ctggcaaata acgtgatggc gcaatctgaa atcgaggccg gacgtcttgt
     5641 ttgcccgttt aatgatgttc tggtcagtaa aaatgctttt tatctggttt gtcatgacag
     5701 ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg atcctggcga aagccgctgc
     5761 tgaacaagaa aaattccgct ttcgttatga acaataattt acgtagggta cgaccatgac
     5821 cagccgtttt atgctgattt tcgccgccat tagcggcttc atttttgtgg cactgggcgc
     5881 ttttggtgcg catgtgttaa gtaaaaccat gggggccgtt gagatgggct ggatccaaac
     5941 cggtctcgaa taccaggcgt ttcatacgct ggcgatctta ggtctggcgg tggcaatgca
     6001 gcgtcgcatc agtatctggt tttactggag tagcgttttc ctcgcgttag gcacggtgct
     6061 gttcagcggc agcctttatt gcctggcgct gtcccatctg cgtttgtggg cgtttgtcac
     6121 accggttggc ggcgtgagct tccttgcggg ctgggcgtta atgttagttg gtgctatccg
     6181 tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt gctgtgccgt ccgggctttg
     6241 aaaaagagtg cgccgcagaa attaccgata aagccggcca gcgggaaatt ttcggttttg
     6301 cccgcgtgaa agagaatgcg ggttatgtca tttatgaatg ttatcaacct gatgatggcg
     6361 ataagttaat ccgtgagctg ccgttcagtt cattaatttt tgcccgccag tggtttgtgg
     6421 tgggggaact cctgcagcat ttgccgccag aagatcgtat tacccccatt gtcggcatgt
     6481 tacagggtgt agtagagaag ggcggtgaac tgcgtgttga agttgccgat accaacgaaa
     6541 gcaaagagtt gctgaaattc tgccgtaagt tcactgttcc gctacgcgct gccttgcgcg
     6601 atgctggggt gctggcgaac tatgaaacgc cgaagcgtcc ggttgtgcat gtattcttta
     6661 ttgcaccagg atgctgctat accggttact catacagcaa caataattcg ccgttctata
     6721 tgggcattcc gcgcctgaaa tttccggcag aggcgccgag tcgttccacg ctcaaactgg
     6781 aagaggcatt tcatgtgttt attcctgcgg atgagtggga tgaacgcctg gcgaacggga
     6841 tgtgggcggt ggatttaggc gcttgccctg gcggctggac ctaccaactg gtgaagcgca
     6901 acatgtgggt ttattccgtc gacaacggcc cgatggcgca aagtctgatg gataccgggc
     6961 aggtgacgtg gctgcgggaa gacggtttca aattccgtcc gacacgcagc aatatctcct
     7021 ggatggtatg cgatatggtt gaaaaaccgg ctaaagttgc ggcattgatg gcgcagtggc
     7081 tggttaatgg ctggtgccgt gaaaccattt tcaatctcaa actgccgatg aaaaaacgct
     7141 acgaagaagt atcacacaat ctggcgtata ttcaggcaca gcttgatgaa catggcataa
     7201 atgctcagat tcaggcgcgg cagttgtatc acgatcgcga agaagtgacg gtgcacgtcc
     7261 gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg ataacaggca aggccggggc
     7321 gtcgtctccg gcctgctacc ctttccgtca ggctgttacc aaagaagttg caacctggcg
     7381 ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg atgcgggctt cccctgaacg
     7441 attaaattta cttttatcaa tcaataacaa cgattgcgcg gcacgtttta atagcatcga
     7501 tttgtaatca gcgttgatcg cattggagtc ccacagtgcg ccgctgctat cgatcccttc
     7561 acaagaaaaa ataaacagat cgatttccag cgatttaagt tgggaaatca gcgagggatt
     7621 gacgtaacag ccatatttgc gctcaagtgt gccgccggaa ctgatcagtt gaatgcgttc
     7681 gcgtttaccg agttcatggc aaatcggatg gctattggtg aagacctgaa tgttgatgtc
     7741 aggcaactgg cgtgccagat accagcaagt tgaactggcg tctaaggcta tcaccatccc
     7801 ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct ttatgcgcat aatggctttt
     7861 cagcctgatg tgaaagggat cgccactgtc ttgattttga cggtggatat atttagcgcg
     7921 tccatgattg cgcagaattt taccctgcgt ctgtaattca ttgagatcgc gacgaatggt
     7981 ttctttactg acctttagct gttcagagag agcttccgtg gtcaggctgg tatggttcag
     8041 cagcaggtcg actatcgctt gctggcgtgc cgctttcata tttttcaccc catcattcga
     8101 taaccgcccg cgtcatgcgg gcgtaccggt atgagattac ggtgttaccc ctttttttaa
     8161 aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg atcgcaaagg ctttttgcgc
     8221 ccgttcataa aacgcaaaac gattgatgcg gatgatgtca ggacacgggg cttgtagtga
     8281 aagcgcatta cggtaacgtc gttctacttc aggatcgaga gtgtcaccct ctaccgccgc
     8341 catcatcacc agcggcgggg cataactgtc cagctcaaat aacgggataa tcgcctggag
     8401 caagtcgctc accaacaggc catcagcgcg gatcacctgc ggttccatcg aatgggcggg
     8461 aaagtgagca tcggaaaaaa taatttcatc tccatgtccc atctctgcca gcactttcaa
     8521 tagttcggga gaaattaacg gcgaaattgt tttcagcatt tcacacttcc tctataaatt
     8581 caggttcagt ttgcgggtag aaataacggt actgataatg aatctgtgca cgggcttctt
     8641 ccgggctgtt aaattcccct acgccatacc aaccgaacag cgcagctcct gcgacggtcg
     8701 tttcggcatc gtcgagaact tttaccggaa tatcaagcat attggcttta atctgattcc
     8761 acaatgtgtt gcgacttcct ccaccgacta acaataattc ataggcttta aagtgcccga
     8821 ttttttccag catctgtaga ttgcgctgta attgcgcagt taacccttcc agcgccgcgc
     8881 gatagaaatg cccccgcgtg gtattaagcg tcactccttg ccagccagcg ttctgacacg
     8941 acaataaatc acactgcatt ttcacaccat ccgcgccagg tgcgatcaga cgagcttctt
     9001 caatcaacat ttgccagggt gtttcagccg tccagaacag ttttctcacc cattccagca
     9061 cgccggatgc cagccattgc atacctgggt tatacaacca tgcctggcta tctagttcgc
     9121 aggtggaacc ggcgtactga cttaacagcg aagtatcaac ctaggcgctg cgaaccatta
     9181 aaatttccca tgtaccggag gagagcacgg gttcattttg ttcggcacca gcgccaaaaa
     9241 gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat gccaacgggt aagccgagca
     9301 ttgctgcggc gttgttctgt agcgtaccaa tctgttcacc cgcttccacc agacgaggga
     9361 agagtcggcg tggaataccg gtggcttgta aaatttgcgg actgaaatcg cgttgctgga
     9421 tatccagcat ctggctggtt ccggccatcg tgatatcagt agtgaattcg ctggttaaac
     9481 ggtggttaat cagcgacgaa atgaagagcc aggcgtgcgc gcgttccagc agttgtggat
     9541 gattttcttt caaccacacc aacttatata acgtattgaa actaaaggct ccgacgccag
     9601 aaatagcctg caaccgctgt gcggagatta accgttcaat attgtccata accgctgctg
     9661 ttcgcggaca tttccagcta atgatcggat agagcagatt gccttgctta tctaccagag
     9721 cgccatccac actaaaggtg gtgacggcga taccgcggat gtggcattca gtcagttcac
     9781 tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat ggcagtctaa agaccactgg
     9841 tgccaggtgt tgttttccat cgcgatatcg ctggcattag gcgttgaggc gcgggcaaca
     9901 atttttccct gccgattaac cgcgatggcc ctgacattgg tcgcgccaca gtcgagtacc
     9961 aggataactt cttgtttcat aatcgctcct gcaatatagc cggataacat tgtttatccg
    10021 gctaaccact cttgcttgtt caggcccggt agccggagcg accgggcatc acatcagggt
    10081 gtaatgtatt aacgcttgta caacggaccg tagttctggc aagcgcggta atcctggcct
    10141 tcaatatcca tgccgtgcgc agcccaggca gaaggacgat acactttggt ctcttcaacg
    10201 ttgtgcatac ataccgggat acgcagcatg gaggcaaggg tgataaagtc tgcgccaacg
    10261 tggccgatgg tcagaactcc gtggttggca ccccagttcg ccattaccga atacacatcc
    10321 gtaaacggcc ctttaccagt gaggcgcggt gcaaaccagg tggttggcca ggttgagttg
    10381 gtgcgtttgt tgaggatgtc atgcacatcc ttcggcaatt ccacgctcca gccttccgcg
    10441 atttgcagta ccggtcccag gcctttgatg atgttgacac gagtcatggt gaacgggacg
    10501 ccgccttcgg taaggaagcg ggaagagtaa ccgccgccac ggaagtattc gtggatcgcc
    10561 gggcaccatt cggtagcggc gaggcaagcg tcagcctctt gctgagagat ttcccagtgc
    10621 ggcttcatcg tcgggttacc ttcgctgtcg cgttgtttac aggaaccgtc cagcgcagca
    10681 gaacctgagt tgatcaaatg gatgatgccg tgttctgcca gtccgtccag tttatgcccc
    10741 gttacacgct caattgcttc tggtgaccag taggtacgca catcggcaaa tacctgagcg
    10801 gtgccggtga gctggtgacc cattagcatt gccacgccgt taagactgtc gttttcggtc
    10861 gccacgacaa agggttcgcg cacgccattc cagtcaaatg aactgttgag gatcgcttcg
    10921 gcggtgtcac cattgggata ttgatcggtc cagtgacgtt gcccctggaa gcccgcagcg
    10981 atggcgttgt agccaagtga ttcttccacg cgaccaatat cggccagttt gctgttgcct
    11041 tgcatcatgt cgcggataca catcgccatc agtaaacttt cgcgcagaac tgcgcggctt
    11101 tgctcggcat tgcgttgata ctgtttgtta ttttcatctt cgccatagcg gaagttttta
    11161 tcagcccagg ccagtgccat ttccagttcg gcttcgtcgt aaatcttctg atcgatacgg
    11221 cgacgcagtt cggtcatatc caccgcctgg actttcattc ccagccagga ttcaaagaag
    11281 ttgtgatcaa caatagaacc ggcgataccc atcgaaacgc cgcccagcga cagatagctt
    11341 ttacctttca tgctggcgac ggccaaaccg gcgcgggcaa agcgcagcag tttttcttca
    11401 acatcggcag gaatcgatgt gtcatcggca tcctgaacgt catgaccgta aatggagaat
    11461 gccgggatgc ctttctggct gtgagctgcc agagccgctg ccaggtaaac agcgccgggg
    11521 cgttcagtgc cgttaaagcc ccaaatggct ttcgggcggg ttggatccat gtcgatggtt
    11581 tcactgccat agcaccagca aggcgttacc gtaatggtga ggcctacatt ctggctgctg
    11641 aatttttctt cgcaagcagc ggcttcagcc atacccgcga tacaggtatc ggaaatgaca
    11701 cactcgacgg cagctccgca ggcatggcgc agtttctcgg tcagcagtgc ggccgtagct
    11761 ttcgccatat tcattgtttg ttcttcaagc gactcacgaa cacccatgcg acgaccgtca
    11821 ataaccgggc ggataccaat tttcggtaag ctgatttttt tcattacaga ttcctcactt
    11881 tattcggaaa atatgttcag ttagttgccg tttgagaacg gaaacgggca aagataaaga
    11941 tgaccgcgaa gcagagtgcg gggatcagtt cagcagtggg gatgttgccc gccgcgtcac
    12001 tgacaaaacc catgaccgga gtgacaatac cgccgccaat aatggtcata acgatgaagg
    12061 acgaaccgta tttggtgtcc tggccgagat tcttaatgcc cagcgagaag attgttgggt
    12121 actgaatcga cataaaggcg ctgcataaag tcagggctat taagcccaca tgaccgccag
    12181 cgaaggctga gatcaggcac agtgccatag cgattaatgc gtaggcggcc aggactttgt
    12241 gtggtgcgaa gcgactgatg agccaggtac cggtgaaacg accaataaag aagcacacca
    12301 tggttccggt taaatagtta gcggcaaagc ctgcagtcat acctggaatt tcttctacag
    12361 cgtagcgaat caaatagctc cagcaggccg tttgtgcgcc gacatagcag aattgcgcta
    12421 ataccgccca gcgccagtga cgaatacgcg ccagacgaga aagcgatgcg gagaacgatc
    12481 cttgtttggc gtcactgtga ttatcactct gcaatgccgg gaatttcgtc agcatgatca
    12541 gcagggcgac cagtaacacg atagccacga tgatcatata aggtgtctgt accgataata
    12601 ccaggctgtg tttatacgca ctcaattgct ctggagacat tttatcgaga acgtcttgcg
    12661 attgatgtgg cacgttagac aaaataagac tttgcccaaa gacaaccgcg ataattgcgc
    12721 caaacgagtt aaatgtttgc gcaagattta agcggaagtg actactgctt tccggcccta
    12781 ataccgtaat aaaagggttt gcggcagttt ccagacaacc taatccggct gcaataataa
    12841 ataggccaac taaaaataag gtgtagttca ttatttctgc agcgggccag aataatgcag
    12901 cgcccaaggc atataaaaat aatccggtaa taatccctgc tttataactg agttttttca
    12961 tcaatatccc agcagggatt gggataatga aataaccaaa gtaaaaggcc gattggatca
    13021 ggccagcctg gaaatttgtc agcgtaaaag cctgctggaa ttgaggtaat aaaatgtcgt
    13081 taaggttatt ggctaccgcc caaagaaaaa acagtgagca cagcagcgcg aatggaataa
    13141 tgtaacttct gctttgccct gcatctttat ctaccgcacg gtaactctgc gtttgtattg
    13201 atgtgtttcc catagcatcc tcttaggttc agaagcttaa gcacgtaatc ggttatgttt
    13261 tcaaaagtgc aaaaccagcg agttcgggct tcatatcggt gaatatttgc ctgtctgaaa
    13321 tgaactcatg gattcatttt caaataaaaa acgctctgta atatgacggc ggtcacactt
    13381 attagctgga aggtgacttt atgtgactac catcacttta ataatcgaaa tttcatgccc
    13441 gtttcagaaa ataaatcata aaaaacacaa accagatgac cgtattaatt gtttgtgccg
    13501 aaaacgggca tttctggcga ggggaaatgg aaaaatgacc gttttgataa atatttttaa
    13561 gagcggtcat ttttaacggg attataaaaa gagaacttgc agttatttac tgtgattagt
    13621 tgagctaggt cacaaaatat aagcttgtga atttctttct tgttaatcag gaagaagatg
    13681 gtgggtaatt aaacggctaa ttcaatagtg tgaaaggaac aacattattg ccctgttttg
    13741 aatcagagag aggtagctaa tggaacgaaa taaacttgct cgtcagatta ttgacacttg
    13801 cctggaaatg acccgcctgg gactgaacca ggggacagcg gggaacgtca gtgtacgtta
    13861 tcaggatggg atgctgatta cgcctacagg cattccatat gaaaaactga cggagtcgca
    13921 tattgtcttt attgatggca acggtaaaca tgaggaagga aagctcccct caagcgaatg
    13981 gcgtttccat atggcagcct atcaaagcag accggatgcc aacgcggttg ttcacaatca
    14041 tgccgttcat tgcacggcag tttccattct taaccgctcg atccccgcta ttcactacat
    14101 gattgcggcg gctggcggta attctattcc ttgcgcgcct tatgcgacct ttggaacacg
    14161 cgaactttct gaacatgttg cgctggctct caaaaatcgt aaggcaactt tgttacaaca
    14221 tcatgggctt atcgcttgtg aggtgaatct ggaaaaagcg ttatggctgg cgcatgaagt
    14281 tgaagtgctg gcgcaacttt acctgacgac cctggcgatt acggacccgg tgccagtgct
    14341 gagcgatgaa gagattgccg tagtgctgga gaaattcaaa acctatgggt tacggattga
    14401 agagtaattt cgtaaagcaa caaggagaag gatgatggct aacagaatga ttctgaacga
    14461 aacggcatgg tttggtcggg gtgctgttgg ggctttaacc gatgaggtga aacgccgtgg
    14521 ttatcagaag gcgctgatcg tcaccgataa aacgctggtg caatgcggcg tggtggcgaa
    14581 agtgaccgat aagatggatg ctgcagggct ggcatgggcg atttacgacg gcgtagtgcc
    14641 caacccaaca attactgtcg tcaaagaagg gctcggtgta ttccagaata gcggcgcgga
    14701 ttacctgatc gctattggtg gtggttctcc acaggatact tgtaaagcaa ttggcattat
    14761 cagcaacaac ccggagtttg ccgatgtgcg tagcctggaa gggctttccc cgaccaataa
    14821 acccagtgta ccgattctgg caatccccac cacagcaggt actgcggcag aagtgaccat
    14881 taactacgtg atcactgacg aagaaaaacg gcgcaagttt gtttgcgttg atccgcatga
    14941 tatcccgcag gtggcgttta ttgatgctga catgatggat ggtatgcctc cggcgctgaa
    15001 agccgcgacg ggtgttgatg cgctcactca tgctattgag gggtatatta cccgtggcgc
    15061 gtgggcacta accgatgcac tgcacattaa agcgattgaa atcattgctg gggcgctgcg
    15121 aggatcggtt gctggtgata aggatgccgg agaagaaatg gcgctcgggc agtatgttgc
    15181 gggtatgggc ttctcgaatg ttgggttagg gttggtgcat ggtatggcgc atccactggg
    15241 cgcgttttat aacactccac acggtgttgc gaacgccatc ctgttaccgc atgtcatgcg
    15301 ctataacgct gactttaccg gtgagaagta ccgcgatatc gcgcgcgtta tgggcgtgaa
    15361 agtggaaggt atgagcctgg aaggggcgcg taatgccgct gttgaagcgg tgtttgccct
    15421 caaccgtgat gtcggtattc cgccacattt gcgtgatgtt ggtgtacgca aggaagacat
    15481 ttcggcactg gctcaggcgg cactggatga tgtttgtacc ggtggcaacc cgcgtgaagc
    15541 aacgcttgag gatattgtag agctttacca taccgcctgg taaatgcgct gatgtgataa
    15601 tgccggatac gatttgcggc gtatccggct cgccgttacc gtaccaaccg caattgctgt
    15661 aaattgccgt cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca cagaaacgcc
    15721 atctctttat gggtttctaa ttttttgcgc cacttttcgg caaccgcatc cagattctca
    15781 tatatccctt ccagactctg aaactcgacc agcagctgcg tggcgctttt tggtccgatt
    15841 cccgcaacac ccggtacctt tgaactgctg atccccgcaa gtccccagta atcgggcaac
    15901 tgctgcggtt gaacgccaaa ttctttatcg ataaatggcg catccagcca acgtttctgg
    15961 aagtaatcac gaatacgtaa tgtcggtgaa agtaactgac agtagccttt atctgtcgaa
    16021 acaatcgttg cctgatgccc ggcctgtgtc actttgaccg ccagtgtggc ggctaagtca
    16081 tcggcttcgt tgccgctggc tgaccagcac gggacgccgc gttgctcaaa ggcggcgcgt
    16141 aatgcaggca tctcgtcgtg caactcttcc ggcattggcg gtcgatccgc tttgtaatct
    16201 ggtaaacgct gatgacgcca gccgctactg cggttttcat catcaaaaac ggcgaccgcg
    16261 tgggttggct ggctgtgcat aatgagctga tcgagcgcat gctggcaggt ttcgacacag
    16321 ggcgaaccct gaacggcatg aatgcgacgg ataagattca gtgcatcgac aataagcaaa
    16381 tgaacagcca cgataacccc cttaaagatc aggcgataag ggtaacattc gatgccggaa
    16441 caggctatgg ctggttgaga aaacaggaag aggcttcgca aaacgaggcc tctgggagag
    16501 attaatcgca ggcaacgatc ttcatcgcca ggccgccgcg agaggtttcg cggtacttgg
    16561 cgttcatgtc tttacctgtt tcgtacatgg tttcgataac tttatcgagg cagacgcgcg
    16621 gttcgctggt acggcgcagt gccatacgcg cggcgttcac cgctttcact gctgcaatgg
    16681 cgttacgctc aatgcacggt acctgaacct gccccgcgac cgggtcacaa gtcagaccga
    16741 ggttgtgctc catcgcgatt tccgctgcga tgcaaacctg cgccgggctt gcgcctaaca
    16801 gttctgccag accagctgcc gccatagagc acgccacgcc aacttcaccc tggcaaccca
    16861 cttcggcacc agaaatcgac gcgttcatct tataaagaga accaatggcg ctggctacca
    16921 gcaggtaacg agccagtgag ttagcgttca cttcgcggat aaacttatcg tagtacgcca
    16981 gcacagccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg cgaccgccag
    17041 cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca accgccatcg
    17101 ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca gcacggcgtg
    17161 gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca ataccgccac
    17221 gcatgacttc ccagacgttc gccaggtgct gttccagctc ttctttactg tgcagcgcca
    17281 gctcgttttt catcatcagg ccagagagtg acagcccggt ttcctgacaa tgtttttgca
    17341 gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctatcc tgctggccaa
    17401 aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctgg ctgtaaacga
    17461 ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa aggttgtctg
    17521 catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg tttgccagca
    17581 tcaggcgacc atgagtattc acatcctgaa taaaaccggg gatggaatcg atatccacgg
    17641 tatccggcag gttacccgcc aggcccataa taatggcgat atctgtgtgg tggcctttac
    17701 cagtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct ttaagcaggt
    17761 tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca acggtatgag
    17821 aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata ggaaatacat
    17881 cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag ctgaacagag
    17941 agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg ttgctgatgt
    18001 gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc atcaggaaca
    18061 ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg ctcgggttca
    18121 gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg ttcagcttgt
    18181 tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg ttgaagcctt
    18241 cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc gcgataatcg
    18301 gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac agaatcgaga
    18361 tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtacg cagctgaaga
    18421 cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc ttcgagcatt
    18481 tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa gagatgatcg
    18541 gagagtggtt gaacgagaac accattaccg gaattgccag ccacagggtc atccacagac
    18601 cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg ccgttccact
    18661 gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat accagaatac
    18721 tcatcgcttt aacgatcatc tgctcaccaa agcgaacgat ggtcatcata cccacgatca
    18781 ggatcagcga cagaatcgca cgcggcggtg gcgtcatacc cagctggtga gacatgaagc
    18841 tttcaacggt attggtgatt gccacgctat aaaccagcag gatcgggtag atagcgaaga
    18901 agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct acaacctcgg
    18961 tgatgtcttc gcccgggttt ttaccagaca gtacgaagcg agtcaggccg cggtgagcaa
    19021 aaaacgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata ccgccaacac
    19081 cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa aggcccagca
    19141 tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca atcgtgctgg
    19201 tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct tttagccagt
    19261 tcagcccggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga tggcttaatc
    19321 atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta tctatcagtg
    19381 atcttgatcc caaatacaat aatctgcttg gaagatagat tatcccaacg ctttactctg
    19441 taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga catctgactt
    19501 cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt tagttaatca
    19561 tggcgataat ttctttttca tagcgataaa atacggttta tgaaaatagg gaaacgtttg
    19621 tgttgcaaca taaattgtat gacatattaa ctgtgatgat atttgtcgcg tgagggtggt
    19681 aagaaacatt catataacaa atgaaatgag ttgatgagta agtgaccaaa aacgagaaaa
    19741 cccggcaa
//
LOCUS       DAJTOW010000075        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTOW010000075 DAJTOW010000000
VERSION     DAJTOW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.075"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO244_002763"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO244_002763"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5664521.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO244_002764"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO244_002764"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5664522.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO244_002765"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO244_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5664523.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO244_002766"
     CDS             6038..6658
                     /locus_tag="OO244_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5664524.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO244_002767"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO244_002767"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5664525.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO244_002768"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO244_002768"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5664526.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKIFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO244_002769"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO244_002769"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5664527.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO244_002770"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO244_002770"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5664528.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO244_002771"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO244_002771"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO244_002772"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO244_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO244_002773"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO244_002773"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5664529.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO244_002774"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO244_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5664530.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO244_002775"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO244_002775"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5664531.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO244_002776"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO244_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5664532.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO244_002777"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO244_002777"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5664533.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO244_002778"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO244_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5664534.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4680 a         5505 c         5033 g         4480 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaagattttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctgggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 agcccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcatcgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aatcgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTOW010000076        19308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTOW010000076 DAJTOW010000000
VERSION     DAJTOW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.076"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO244_002779"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO244_002779"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5664535.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO244_002780"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO244_002780"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5664536.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO244_002781"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO244_002781"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5664537.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO244_002782"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO244_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5664538.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO244_002783"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO244_002783"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5664539.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO244_002784"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO244_002784"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5664540.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO244_002785"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO244_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5664541.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO244_002786"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO244_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5664542.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO244_002787"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO244_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5664543.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO244_002788"
     CDS             11296..11511
                     /locus_tag="OO244_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5664544.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO244_002789"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO244_002789"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5664545.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO244_002790"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO244_002790"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5664546.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO244_002791"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO244_002791"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5664547.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO244_002792"
     CDS             complement(16247..16786)
                     /locus_tag="OO244_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5664548.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO244_002793"
     CDS             complement(16802..17320)
                     /locus_tag="OO244_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5664549.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO244_002794"
     CDS             complement(17631..17822)
                     /locus_tag="OO244_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5664550.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO244_002795"
     CDS             17840..17992
                     /locus_tag="OO244_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664551.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..>19308
                     /gene="pdeF"
                     /locus_tag="OO244_002796"
     CDS             18174..>19308
                     /gene="pdeF"
                     /locus_tag="OO244_002796"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5664552.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4546 a         4700 c         5249 g         4813 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgattggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctacca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaat
//
LOCUS       DAJTOW010000077        19284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTOW010000077 DAJTOW010000000
VERSION     DAJTOW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.077"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            2..1498
                     /gene="ravA"
                     /locus_tag="OO244_002797"
     CDS             2..1498
                     /gene="ravA"
                     /locus_tag="OO244_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5664553.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1492..2943
                     /gene="viaA"
                     /locus_tag="OO244_002798"
     CDS             1492..2943
                     /gene="viaA"
                     /locus_tag="OO244_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5664554.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVMISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2948..3940)
                     /gene="asnA"
                     /locus_tag="OO244_002799"
     CDS             complement(2948..3940)
                     /gene="asnA"
                     /locus_tag="OO244_002799"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5664555.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4092..4550
                     /gene="asnC"
                     /locus_tag="OO244_002800"
     CDS             4092..4550
                     /gene="asnC"
                     /locus_tag="OO244_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5664556.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4640..5083
                     /gene="mioC"
                     /locus_tag="OO244_002801"
     CDS             4640..5083
                     /gene="mioC"
                     /locus_tag="OO244_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5664557.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5462..7351
                     /gene="mnmG"
                     /locus_tag="OO244_002802"
     CDS             5462..7351
                     /gene="mnmG"
                     /locus_tag="OO244_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5664558.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7415..8038
                     /gene="rsmG"
                     /locus_tag="OO244_002803"
     CDS             7415..8038
                     /gene="rsmG"
                     /locus_tag="OO244_002803"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5664559.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8655..9035
                     /gene="atpI"
                     /locus_tag="OO244_002804"
     CDS             8655..9035
                     /gene="atpI"
                     /locus_tag="OO244_002804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5664560.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9044..9859
                     /gene="atpB"
                     /locus_tag="OO244_002805"
     CDS             9044..9859
                     /gene="atpB"
                     /locus_tag="OO244_002805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5664561.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNTYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9906..10145
                     /gene="atpE"
                     /locus_tag="OO244_002806"
     CDS             9906..10145
                     /gene="atpE"
                     /locus_tag="OO244_002806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5664562.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10207..10677
                     /gene="atpF"
                     /locus_tag="OO244_002807"
     CDS             10207..10677
                     /gene="atpF"
                     /locus_tag="OO244_002807"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5664563.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10692..11225
                     /gene="atpH"
                     /locus_tag="OO244_002808"
     CDS             10692..11225
                     /gene="atpH"
                     /locus_tag="OO244_002808"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5664564.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11238..12779
                     /gene="atpA"
                     /locus_tag="OO244_002809"
     CDS             11238..12779
                     /gene="atpA"
                     /locus_tag="OO244_002809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5664565.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12830..13693
                     /gene="atpG"
                     /locus_tag="OO244_002810"
     CDS             12830..13693
                     /gene="atpG"
                     /locus_tag="OO244_002810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5664566.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13720..15102
                     /gene="atpD"
                     /locus_tag="OO244_002811"
     CDS             13720..15102
                     /gene="atpD"
                     /locus_tag="OO244_002811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5664567.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15123..15542
                     /gene="atpC"
                     /locus_tag="OO244_002812"
     CDS             15123..15542
                     /gene="atpC"
                     /locus_tag="OO244_002812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5664568.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15893..17263
                     /gene="glmU"
                     /locus_tag="OO244_002813"
     CDS             15893..17263
                     /gene="glmU"
                     /locus_tag="OO244_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5664569.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17425..19254
                     /gene="glmS"
                     /locus_tag="OO244_002814"
     CDS             17425..19254
                     /gene="glmS"
                     /locus_tag="OO244_002814"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5664570.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEVASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4738 a         4832 c         5107 g         4607 t
ORIGIN      
        1 tatggctcac cctcatttat tagcggaaag aatttcccgc ctgagcagtt cgttggaaaa
       61 ggggctttat gaacgtagcc acgccatccg cttgtgttta ttagcggcat taagtggtga
      121 aagtgtgttc ctccttggcc cgccaggtat tgccaaaagt ttgatcgccc ggcgcttaaa
      181 attcgccttt cagaatgccc gcgcgtttga atatctgatg acccgcttct ccacgccgga
      241 agaagttttt ggtccccttt ctattcaggc gctaaaagat gaagggcgct atgaacgttt
      301 aaccagcggt tacctgccgg aagcagaaat cgtctttctg gatgagatct ggaaagcggg
      361 cccggcaatt cttaatacct tgctcaccgc cattaacgag cgccagttcc gcaacggtgc
      421 acacgtagaa aaaatcccga tgcgtctact ggtggcggcc tccaacgagc tgccggaagc
      481 agacagcagt ctggaagcgt tatatgaccg catgctgatt cgtctatggt tagataaagt
      541 gcaagataaa gcgaatttcc gctccatgct gaccagtcaa caggatgaaa acgacaatcc
      601 ggttcctgat gccttgcagg tcacagatga agaatatgaa cgctggcaga aagagattgg
      661 tgaaattacg ctgcccgatc atgtatttga gctgattttt attctgcgcc agcaactgga
      721 taaattaccg gatgcgcctt atgtctcgga tcgtcgctgg aaaaaagcga tccgcttatt
      781 gcaggccagc gcctttttta gcggtcgcag tgctgttgcc ccggttgatc tcattttgct
      841 gaaagattgc ctgtggtatg acgcgcaaag cctgaatttg atacaacaac aaattgatgt
      901 attgatgacc ggtcatgcct ggcaacagca agggatgttg acccgcctgg gcgcgattgt
      961 gcaacgtcac ctgcaactcc agcagcaaca aagcgataaa acagccttaa cgttaattcg
     1021 tctgggcggc attttcagcc gtcgtcagca gtatcaactt cctgttaacg ttactgcttc
     1081 cactctgact ctgctgctgc aaaaaccgtt aaaactgcat gatatggaag tggttcatat
     1141 ctcctttgag cgtagcgcgc tggaacagtg gctgagtaaa ggtggtgaaa ttcgcggcaa
     1201 actgaacggt atcggctttg cccagaaact gaatctggaa gttgatagca cccaacatct
     1261 tgttgtacgc gatgtaagtt tacaaggcag tacgctggca cttcccggtt catcggctga
     1321 aggtctgcca ggtgaaataa aacaacaact ggaagagctt gaaagcgact ggcgtaagca
     1381 acacgcttta ttcagcgaac agcaaaaatg tctgtttatc cctggcgact ggttaggtcg
     1441 cattgaagac agcctacagg atgtcggtgc acagattcgt caggcacaac aatgctaacg
     1501 ctggatacgc ttaatgtgat gctggccgtc agcgaagagg gattgatcga agagatgatc
     1561 atcgcactgc tggcctcacc gcagctggcg gtcttctttg aaaaattccc acgactgaag
     1621 gcggcaatca ctgatgatgt tccccgctgg cgtgaggcgc tgcgcagtcg gctgaaagat
     1681 gcccgagtcc cgccggaact caccgaagag gtgatgtgct atcagcaaag ccagctcctc
     1741 tccacgccgc agtttattgt gcagctacca cagatcctgg acttactgca tcgtctgaat
     1801 tccccatggg cagaacaagc ccggcagttg gttgatgcta acagcacaat aacttcagcg
     1861 ttacacacgc tttttctcca gcgttggcgt ttaagtctga tcgtgcaagc aacgacgtta
     1921 aatcaacagc tattagaaga agaacgcgaa caactgttaa gtgaagttca ggaacgcatg
     1981 acgctgagcg gacaacttga accgattctc gcagataaca ataccgcagc tggtcgtctg
     2041 tgggatatga gcgccggtca acttaaacgt ggcgactatc agtttattgt gaaatacggt
     2101 gaatttctta acgaacagcc ggaactgaaa cgcctggcag agcagctggg gcgttctcgg
     2161 gaagccaaat caataccgcg caacgatgcg cagatggaaa ccttccgcac catggtgcgc
     2221 gaaccggcga cggttcctga gcaggttgat ggtctgcaac aaagcgatga tattttacgt
     2281 ctcctgccgc cagaactggc gacactaggg ataacggaac tggagtatga gttttaccgt
     2341 cggctggtgg aaaaacagtt gctcacctat cgcctgcacg gtgagtcgtg gcgtgaaaaa
     2401 gtgttcgaac gtccggtggt acataaagat tacgatgaac agccgcgcgg gccgtttatt
     2461 gtctgtgtgg atacttccgg ctcaatgggc ggctttaatg aacagtgtgc gaaagcgttc
     2521 tgcctggctt tgatgcgcat tgctctcgct gaaaaccggc gctgttatat tatgctattt
     2581 tccaccgaga tcgtccgtta tgagctttca ggcccacaag gcatcgaaca agcaatccgt
     2641 tttttaagcc agcagtttcg tggcggcacc gatcttgcca gttgttttcg cgccattatg
     2701 gaacgcttgc aaagcaggga atggtttgat gccgatgcgg tgatgatttc tgattttatc
     2761 gctcagcggt tgcctgacga cgtgacgagt aaagtgaaag agctgcagcg ggtacatcag
     2821 catcgctttc atgccgtggc gatgtcggca cacggcaaac ccggcatcat gcgcattttc
     2881 gatcatatct ggcgctttga taccgggatg cgaagccgcc tgctcagacg ctggcggcga
     2941 taaattatta cagcagagaa gggacgctct cgcgaacagc agctggccat actccacact
     3001 gaacctggcc gatatgcggc agttgcagca gcagcatagt caaacgagac tggccgatac
     3061 cgccgccgat ggtctgcggc atttcaccgc gcagcagcgc ctgatgccat tccagctgca
     3121 gacgatcttc atcaccggtc agcgccagct gatgcttcag cgtgtcggca tccacgcgga
     3181 tccccatgga agaaagctca aacgcatctt ccagtaccgg gttccacacc agaatatcgc
     3241 cgttcaggcc cgcatggccc agctctgacg gggtgctcca gtcatcataa tccggtgcgc
     3301 gcacgtcgtg acgatgacca tcactcaact tgccgccaat cccgacgagg aataccgcgc
     3361 caagatcttt cgcaatcgcc cgctcacgcc ctttggcatc gagatccgga taacgagaca
     3421 gtaactcctg gctgtgtacg aagtgaatct gatccggcag gaacggtgcc aggccaaact
     3481 cttcgttaac cgcagcttcg gttgctttaa ttcccgccca gatcgcctct accgtgcttt
     3541 tcagagtcga gagttgacgc tcaccgtcgc ccattacgcg ttcccagtcc cactggtcaa
     3601 catagaccga gtgcaacgga gaaagacggt cttcatcggg gcgaagggct ttcatgtgcg
     3661 tgtacagccc ttcgcccgcg ctgaagtcgt gttgccctaa ggtctgacgt ttccacttcg
     3721 ccagtgaatg aaccacttcg aactgggcat caggcagagc tttcactttt acctgcaccg
     3781 ctttttcaca gcccgacaag ttatcctgcg tgccatcccc cacacggcta agaatcggcg
     3841 cctggacttc gatcagcccc agacgttctt ccagttggcg agaaaagtga gatttcacga
     3901 agctaatttg gcgttgtttg gcaatgtaag cggttttcat tttttatact cctgcgtcct
     3961 gttgcttatg attaagcaac aaaaaccgac acacatgcaa taatcattca ataaaaagcc
     4021 taacacactt ttgattcatt aaaaaagacg gctaaaatag aatgaatcat caatctgcat
     4081 aagaaaatcc tatggaaaat tatctgatcg acaatctgga ccgtggcatc ctggaagcat
     4141 taatgggcaa tgcgcgcacc gcttacgccg aactggcgaa acaatttggc gtcagtccgg
     4201 ggacgattca cgttcgagta gagaaaatga agcaggcggg gatcattacc ggggcgcgta
     4261 ttgatgtcag cccgaagcag ctcggttatg acgtaggctg ctttatcggc attatattaa
     4321 agagcgccaa agactaccct tccgcgctgg caaagctgga aagccttgat gaagtcactg
     4381 aagcctatta cacaaccggc cactacagca tctttataaa agttatgtgc cgttcgatcg
     4441 acgctctcca gcatgtactt atcaacaaga tccaaacaat tgatgaaatt cagtccaccg
     4501 agacattgat cgtcctgcaa aacccgatca tgcgtaccat caagccctga tcggcttttt
     4561 taatcccata cttttccaca ggtagatccc aacgcgttca cagcgtacaa tacgccactc
     4621 ttaataaagg tggcggttta tggcagatat cactcttatc agcggcagca ccctcggcgg
     4681 tgccgaatat gtagcagaac acctggctga aaagctggaa gaggtgggtt ttaccaccga
     4741 aacgctgcac ggtccgctgt tggaagattt atctgcctcg gggatctggc tggttatcag
     4801 ctccacccac ggtgccggag atattccgga caacctttct cctttctatg aagcattgca
     4861 ggaacagaag cccgatcttt ctgcggtccg ctttggcgca atcggtattg gcagtcgtga
     4921 atatgacacc ttttgtgggg ctatcgataa actcgaggca gaactcaaaa attccggtgc
     4981 aaaacagaca ggcgaaacac tgaagatcaa cattcttgat cacgacattc cggaagatcc
     5041 ggcagaagaa tggctgggat cgtggattaa tttactcaaa taagtataca gttcgtgcga
     5101 tctactgtgg ataactctgt caggaagctt ggatcaaccg gtagctatcc aaagaacaac
     5161 cgttgttcag tttttgagtt gtgtataacc cctcattctg atcccagctt atacggtcca
     5221 ggatcaccga tcattcacag ttaatgatcc tttccaggtt gttgatctta aaaatcggat
     5281 ccttgttatc cacagggcag tgcgatccta ataagagatc acaatagaac agatctctaa
     5341 ataaatagat cttcttttta atacccagga tcccaggtct ttctcaagcc gacaaagttg
     5401 agtagaatcc acggcccggg cttcaatcca ttttcatacc gcgttatgcg aggcaatcac
     5461 catgttttat ccggatcctt ttgacgtcat catcattggc gggggtcatg caggcaccga
     5521 ggccgcgatg gctgcggcgc gtatgggtca acagactctg cttttgacac acaatatcga
     5581 cactctgggg cagatgagct gcaacccggc gatcggcggt attgggaagg gacatctggt
     5641 aaaagaagtg gatgcactcg gcggtctgat ggcgaaagcg atcgatcaag cgggtatcca
     5701 gtttaggata ctaaacgcaa gcaaaggacc ggcggttcgc gctacccgag ctcaggcgga
     5761 tcgtgtgcgc taccgtcagg cggtacgtac ggcgctggag aaccaaccga acctgatgat
     5821 cttccagcag gcggttgaag atcttattgt cgaaaacgat cgcgtggtcg gtgctgttac
     5881 ccaaatggga ctgaagttcc gtgccaaagc tgtggtgctc accgttggga cgttcctcga
     5941 cggcaaaatt catatcggtc tggataatta cagcggtggc cgtgctggcg atccgccatc
     6001 cattccgctc tctcgccgtt tgcgtgaact gccgctgcgc gttggtcgtc tgaaaaccgg
     6061 tactccgccg cgtattgatg ctcgaaccat cgactttagc gtgctggcgc aacagcatgg
     6121 cgataaccca atgccggtat tctcgtttat gggcaatgcg tccctgcatc cccagcaggt
     6181 gccgtgttat atcactcata ccaacgagaa aacccatgat gtgatccgca gtaacctcga
     6241 tcgtagccca atgtatgcag gggtgatcga aggtgtcggc ccacgctact gcccgtcgat
     6301 cgaagacaaa gtcatgcgct tcgcagacag aaatcagcat cagatcttcc ttgaaccgga
     6361 aggactgacc tctaatgaaa tttatccgaa cggtatctcc accagcctgc cgttcgatgt
     6421 gcagatgcaa atcgtccgct ctatgcaggg gatggaaaac gcgaagatcg tgcgtccggg
     6481 ttatgccatt gagtatgact tcttcgatcc tcgcgacctg aaaccgacgc tggagagcaa
     6541 gtttatccag gggctgttct ttgctggtca gattaacggc actaccggtt acgaagaagc
     6601 cgctgcgcaa ggtttgctgg ccggtcttaa cgctgcccgt ctgtctgctg acaaagaagg
     6661 ttgggctccg gcacgttctc aggcgtatct cggcgtactg gttgatgacc tgtgcacttt
     6721 aggaaccaaa gaaccgtatc gtatgtttac ttcgcgcgca gaatatcgtc tgatgctgcg
     6781 cgaagataat gcggatctgc gtttgactga aatcggtcgt gaactgggcc tggtggatga
     6841 cgaacgttgg gcgcgcttta acgagaaact tgagaatatc gagcgtgagc gtcagcgtct
     6901 gaaatcgacc tgggtaaccc cgtcggcgga agctgcagcc gaagtgaatg ctcacctgac
     6961 tgcgccgctt tcccgtgaag ccagtggtga agatctgctg cgtcgtccgg aaatgactta
     7021 tgaaaaatta accacgctga cgccgtttgc ccctgcgttg acagacgaac aggcggcgga
     7081 acaggttgag attcaggtta aatacgaagg ttatatcgcg cgccagcaag atgagatcga
     7141 aaagcagctg cgtaacgaga acaccctgct acccgcgaca ctggattacc gccagatatc
     7201 cggtctttct aacgaagtga tcgccaaact taacgatcac aaaccggcct ctatcggcca
     7261 ggcttcgcgt atttctggcg tcacgcctgc ggccatctcc attctgctgg tgtggctgaa
     7321 aaaacagggt atgctgcgtc gtagcgcata acgcattaaa aatgcctggt aagcacccgc
     7381 ttaccaggca acgcatcaag aacaggtaat caccgtgctc aacaaactct ccttactgct
     7441 gaaagacgca ggtatttcgc ttactgatca ccagaaaaac cagcttattg cctacgtgaa
     7501 tatgctgcat aaatggaaca aagcgtacaa cctgacttcg gtccgcgatc ctaatgagat
     7561 gctggtacgc catattctcg atagcattgt ggtggcaccg tatctgcaag gtgaacggtt
     7621 tatcgatgtc ggcaccggac caggactgcc aggcattcca ctctctatcg tgcgtcctga
     7681 agcccatttc actctgttgg atagccttgg taaacgcgtg cgtttccttc gtcaggtgca
     7741 acatgagctt aaactggaga atattgaacc agtacagagc agggtagaag agtttccttc
     7801 agagccgcca tttgatggcg taattagccg cgcttttgcc tctctgaacg atatggtgag
     7861 ctggtgccac catcttcctg gtgagcaagg ccgtttctac gcgctgaaag ggcaaatgcc
     7921 ggaagatgaa atcgctttgt tgcccgaaga atatcaggtc gaatcagtgg ttaaacttca
     7981 ggttccagcc ctggatggcg aacgtcatct ggtggtgatt aaagcaaata aaatttaatt
     8041 tttatcaaaa aaatcataaa aaattgaccg gttagactgt taacaacaac caggttttct
     8101 actgatataa ctggttacat ttaacgccac gttcactctt ttgcatcaac aagataacgt
     8161 ggcttttttt ggtaagcaga aaataagtca ttagtgaaaa tatcagtctg ctaaaaatcg
     8221 gcgctaagaa ccatcattgg ctgttaaaac attattaaaa atgtcaatgg gtggtttttg
     8281 ttgtgtaaat gttatttatt aaaacagtat ctgtttttag actgaaatat cataaacttg
     8341 taaaggcatc atttgccaag taaataaata tgctgtgcgc gaacatgcgc aatatgtgat
     8401 ctgaagcacg ctttatcacc agtgtttacg cgttatttac agtttttcat gatcgaacag
     8461 gggtagcgga aaagtcgcaa ttgtatgcac tggaaaaata tttaaacatt tattcacctt
     8521 ttggctactt attgtttgaa atcacggggg cgcaccgtat aatttgaccg ctttttgatg
     8581 cttgactcta agccttaaag aaagttttat acgacacgcg gcatacctcg aagggagcag
     8641 gagtgaaaaa cgtgatgtct gtgtcgctcg tgagtcgaaa cgttgctcgg aagcttctgc
     8701 tcgttcagtt actggtggtg atagcaagtg gattgctgtt cagcctcaaa gaccccttct
     8761 ggggcgtctc tgcaataagc gggggcctgg cagtctttct gcctaacgtt ttgtttatga
     8821 tatttgcctg gcgtcaccag gcgcatacac cagcgaaagg ccgggtggct tggacattcg
     8881 catttggcga agctttcaaa gttctggcga tgttggtgtt actggtggtg gcgttggcgg
     8941 ttttaaaggc ggtattcttg ccgctgatcg ttacgtgggt tttggtgctg gtggttcaga
     9001 tactggcacc ggctgtaatt aacaacaaag ggtaaaaggc atcatggctt cagaaaatat
     9061 gacgccgcag gattacatag gacaccacct gaataacctt cagctggacc tgcgtacatt
     9121 ctcgctggtg gatccacaaa accccccagc caccttctgg acaatcaata ttgactccat
     9181 gttcttctcg gtggtgctgg gtctgttgtt cctggtttta ttccgtagcg tagccaaaaa
     9241 ggcgaccagc ggtgtgccag gtaagtttca gaccgcgatt gagctggtga tcggctttgt
     9301 taatggtagc gtgaaagaca tgtaccatgg caaaagcaag ctgattgctc cgctggccct
     9361 gacgatcttc gtctgggtat tcctgatgaa tctgatggat ttactgccta tcgacttgct
     9421 gccgtacatt gctgaacatg tactgggtct gcctgcactg cgtgtggttc cgtctgcgga
     9481 cgtgaacgta acgctgtcta tggcactggg cgtatttatc ctgattctgt tctacaacat
     9541 caaaatgaaa ggcatcggcg gcttcacgaa agagttgacg ctgcagccgt tcaatcactg
     9601 ggcgttcatt cctgtcaact taatccttga aggggtaagc ctgctgtcca aaccagtttc
     9661 actcggtttg cgactgttcg gtaacacgta tgccggtgag ctgattttca ttctgattgc
     9721 tggtctgttg ccgtggtggt cacagtggat cctgaatgtg ccgtgggcca ttttccacat
     9781 cctgatcatt acgctgcaag ccttcatctt catggttctg acgatcgtct atctgtcgat
     9841 ggcgtctgaa gaacattaat ttaccaacac tactacgttt taactgaaac aaactggaga
     9901 ctgtcatgga aaacctgaat atggatctgc tgtacatggc tgccgctgtg atgatgggtc
     9961 tggcggcaat cggtgctgcg atcggtatcg gcatcctcgg gggtaaattc ctggaaggcg
    10021 cagcgcgtca acctgatctg attcctctgc tgcgtactca gttctttatc gttatgggtc
    10081 tggtggatgc tatcccgatg atcgctgtag gtctgggtct gtacgtgatg ttcgctgtcg
    10141 cgtagtaagc gttgctttta tttaaagagc aatatcagaa cgttaactaa atagaggcat
    10201 tgtgctgtga atcttaacgc aacaatcctc ggccaggcca tcgcgtttgt cctgttcgtt
    10261 ctgttctgca tgaagtacgt atggccgcca ttaatggcag ccatcgaaaa acgtcaaaaa
    10321 gaaattgctg acggccttgc ttccgcagaa cgagcacata aggaccttga ccttgcaaag
    10381 gccagcgcga ccgaccagct gaaaaaagcg aaagcggaag cccaggtaat catcgagcag
    10441 gcgaacaaac gccgctcgca gattctggac gaagcgaaag ctgaggcaga acaggaacgt
    10501 actaaaatcg tggcccaggc gcaggcagaa attgaagccg agcgtaaacg tgcccgtgaa
    10561 gagctgcgta agcaagttgc tatcctggct gttgctggcg ccgagaagat catcgaacgt
    10621 tccgtggatg aagctgctaa cagcgacatc gtggataaac ttgtcgctga actgtaagga
    10681 gggaggggct gatgtctgaa tttattacgg tagctcgccc ctacgccaaa gcagcttttg
    10741 actttgccgt cgaacaccaa agtgtagaac gctggcagga catgctgacg tttgccgccg
    10801 aggtaaccaa aaacgaacaa atggcagagc ttctctctgg cgcgcttgcg ccagaaacgc
    10861 tcgccgagtc gtttatcgca gtttgtggtg agcaactgga cgaaaacggt cagaacctga
    10921 ttcgggtaat ggctgaaaat ggtcgtctta acgcgctccc ggatgttctg gagcagttta
    10981 ttcaccttcg tgccgtgagt gaggctaccg ctgaggtaga cgtcatttcc gctgccgcac
    11041 tgagtgaaca acagctcgcg aaaatttctg ctgcgatgga aaaacgtctg tcacgcaaag
    11101 ttaagctgaa ttgcaaaatc gataagtctg taatggctgg cgttatcatc cgatcgggtg
    11161 atatggtcat tgatggcagc gtacgcggtc gtcttgagcg ccttgcagac gtcttgcagt
    11221 cttaagggga ctggagcatg caactgaatt ccaccgaaat cagcgaactg atcaagcagc
    11281 gcattgctca gttcaatgtt gtgagtgaag ctcacaacga aggtactatt gtttctgtaa
    11341 gtgacggtgt tatccgcatt cacggcctgg ccgattgtat gcagggtgaa atgatctccc
    11401 tgccgggtaa ccgttacgct atcgcactga acctcgagcg cgactctgta ggtgcggttg
    11461 ttatgggtcc gtacgctgac cttgccgaag gcatgaaagt taagtgtact ggccgtatcc
    11521 tggaagttcc ggttggccgt ggcctgctgg gccgtgtggt taacactctg ggtgcaccaa
    11581 tcgacggtaa aggtccgctg gatcacgacg gcttctctgc tgtagaagca atcgctccgg
    11641 gcgttatcga acgtcagtcc gtagatcagc cggtacagac cggttataaa gccgttgact
    11701 ccatgatccc aatcggtcgt ggtcagcgtg aattgatcat cggtgaccgt cagacaggta
    11761 aaaccgcact ggctatcgat gccatcatca accagcgcga ttccggtatc aaatgtatct
    11821 atgtcgctat cggccagaaa gcgtccacca tttctaacgt ggtacgtaaa ctggaagagc
    11881 acggcgcact ggctaacacc atcgttgtgg tagcaaccgc gtctgaatcc gctgcactgc
    11941 aatacctggc accgtatgcc ggttgcgcaa tgggcgaata cttccgtgac cgcggtgaag
    12001 atgcgctgat catttacgat gacctgtcta aacaggctgt tgcttaccgt cagatctccc
    12061 tgctgctccg tcgtccgcca ggacgtgaag cattcccggg cgacgttttc tacctccact
    12121 ctcgtctgct ggagcgtgct gcacgtgtta acgccgaata cgttgaagcc ttcaccaaag
    12181 gtgaagtgaa agggaaaacc ggttctctga ccgcactgcc gattatcgaa actcaggcgg
    12241 gtgacgtttc tgcgttcgtt ccgaccaacg taatctccat taccgatggt cagatcttcc
    12301 tggaaaccaa cctgttcaac gccggtattc gtcctgcggt taacccgggt atttccgtat
    12361 cccgtgttgg tggtgcagca cagaccaaga tcatgaaaaa actgtccggt ggtatccgta
    12421 ccgctctggc acagtatcgt gaactggcag cgttctctca gtttgcatcc gaccttgacg
    12481 atgcaacacg taagcagctt gaccacggtc agaaagtgac cgaactgctg aaacagaaac
    12541 agtatgcgcc gatgtccgtt gcgcagcagt ctctggttct gttcgcagca gaacgtggtt
    12601 acctggcgga tgttgaactg tcgaaaattg gcagcttcga agccgctctg ctggcttacg
    12661 tcgaccgtga tcacgctccg ttgatgcaag agatcaacca gaccggtggc tacaacgacg
    12721 aaatcgaagg caagctgaaa ggcatcctcg attccttcaa agcaacccaa tcctggtaac
    12781 gtctggcggc ttgccttagg gcaggccgca aggcattgag gagaagctca tggccggcgc
    12841 aaaagagata cgtagtaaga tcgcaagcgt ccagaacacg caaaagatca ctaaagcgat
    12901 ggagatggtc gccgcttcca aaatgcgtaa atcgcaggat cgcatggcgg ccagccgtcc
    12961 ttatgcagaa accatgcgca aagtgattgg tcaccttgca cacggtaatc tggaatataa
    13021 gcacccttac ctggaagacc gcgacgttaa acgcgtgggc tacctggtgg tgtcgaccga
    13081 ccgtggtttg tgcggtggtt tgaacattaa cctgttcaaa aaactgctgg cggaaatgaa
    13141 gacctggacc gacaaaggcg ttcaatgcga cctcgcaatg atcggctcga aaggcgtgtc
    13201 gttcttcaac tccgtgggcg gcaatgttgt tgcccaggtc accggcatgg gggataaccc
    13261 ttccctgtcc gaactgatcg gtccggtaaa agtgatgttg caggcctacg acgaaggccg
    13321 tctggacaag ctttacattg tcagcaacaa atttattaac accatgtctc aggttccgac
    13381 catcagccag ctgctgccgt taccggcatc agatgatgat gatctgaaac ataaatcctg
    13441 ggattacctg tacgaacccg atccgaaggc gttgctggat accctgctgc gtcgttatgt
    13501 cgaatctcag gtttatcagg gcgtggttga aaacctggcc agcgagcagg ccgcccgtat
    13561 ggtggcgatg aaagccgcga ccgacaatgg cggcagcctg attaaagagc tgcagttggt
    13621 atacaacaaa gctcgtcagg ccagcattac tcaggaactc accgagatcg tctcgggggc
    13681 cgccgcggtt taaacaggtt atttcgtaga ggatttaaga tggctactgg aaagattgtc
    13741 caggtaatcg gcgccgtagt tgacgtcgaa ttccctcagg atgccgtacc gcgcgtgtac
    13801 gatgctcttg aggtgcaaaa tggtaatgag cgtctggtgc tggaagttca gcagcagctc
    13861 ggcggcggta tcgtgcgtac catcgcaatg ggttcctccg acggtctgcg tcgcggtctg
    13921 gatgtaaaag acctcgaaca cccgatcgaa gtcccggtag gtaaagcgac tctgggccgt
    13981 atcatgaacg tactgggtga accggtcgac atgaaaggcg agatcggtga agaagagcgt
    14041 tgggcgattc accgcgcagc accttcctac gaagagctgt caaactctca ggaactgctg
    14101 gaaaccggta tcaaagttat cgacctgatg tgtccgttcg ctaagggcgg taaagttggt
    14161 ctgttcggtg gtgcgggtgt aggtaaaacc gtaaacatga tggagctcat tcgtaacatc
    14221 gcgatcgagc actccggtta ctctgtgttt gcgggcgtag gtgaacgtac tcgtgagggt
    14281 aacgacttct accacgaaat gaccgactcc aacgttatcg acaaagtatc cctggtgtat
    14341 ggccagatga acgagccgcc gggaaaccgt ctgcgcgttg ctctgaccgg tctgaccatg
    14401 gctgagaaat tccgtgacga aggtcgtgac gttctgctgt tcgttgacaa catctatcgt
    14461 tacaccctgg ccggtacgga agtatccgca ctgctgggcc gtatgccttc agcggtaggt
    14521 tatcagccga ccctggcgga agagatgggc gttctgcagg aacgtatcac ctccaccaaa
    14581 actggttcta tcacctccgt acaggcggta tacgtacctg cggatgactt gactgacccg
    14641 tctccggcaa ccacctttgc gcaccttgac gcaaccgtgg tactgagccg tcagatcgcg
    14701 tctctgggta tctacccggc cgttgacccg ctggactcca ccagccgtca gctggacccg
    14761 ctggtggttg gtcaggaaca ctacgacacc gcgcgtggcg ttcagtccat cctgcaacgt
    14821 tatcaggaac tgaaagacat catcgccatc ttgggtatgg atgaactgtc tgaagaagac
    14881 aaactggtgg tagcgcgtgc tcgtaagatc cagcgcttcc tgtcccagcc gttcttcgtg
    14941 gcagaagtat tcaccggttc tccgggtaaa tacgtctccc tgaaagacac catccgtggc
    15001 tttaaaggca tcatggaagg cgaatacgat cacctgccgg agcaggcgtt ctacatggtc
    15061 ggttccatcg aagaagctgt ggaaaaagcc aaaaaacttt aacgccttaa tcggagggtg
    15121 atatggcaat gacttaccac ctggacgtcg tcagcgcaga gcaacaaatg ttctctggtc
    15181 tggtcgagaa aatccaggta acgggtagcg aaggtgaact ggggatctac cctggccacg
    15241 caccgctgct caccgccatt aagcctggta tgattcgcat cgtgaaacag cacggtcacg
    15301 aagagtttat ctatctgtct ggcggcattc ttgaagtgca gcctggcaac gtgaccgttc
    15361 tggccgacac cgcaattcgc ggccaggatc tcgacgaagc gcgagccatg gaagcgaaac
    15421 gtaaggctga agagcacatt agcagctctc acggcgacgt agattacgct caggcgtctg
    15481 cggaactggc caaagcgatc gcgcagctgc gcgttatcga gttgaccaaa aaagcgatgt
    15541 aacaccggct tgaaaagcac aaaagccagt ctggaaacag gctggctttt tttacgcgtt
    15601 gacccgtcct gaagagcgtt cacatagatc ctgctgatat aaaacacccc tgttttcccg
    15661 tttattcatt gatcgaaata agagcaaaaa catccacctg acgcttaaat gaaggtactg
    15721 ccttaatttt ctgcagataa aaggcgtgac gatggtcgaa aatggcactt tcgtcagcgg
    15781 ggataatccg ttattgaaca atttatcctc tgtccatttc acgatgaaaa aaatgtagtt
    15841 ttttcaaggt gaagcggttt aaattcgttc tcaaattaca gtcaggacgc gtatgttgaa
    15901 taatgctatg agcgtagtga tccttgccgc aggcaaaggc acgcgcatgt attccgatct
    15961 tccgaaagtg ctgcataccc ttgccgggaa agcgatggtt cagcatgtca ttgatgctgc
    16021 gaatgaatta ggcgcagcgc acgttcacct ggtgtacggt cacggcggcg atctgcttaa
    16081 acaggcgctg aaagacgaca accttaactg ggtgcttcag gcagagcagc tgggtacggg
    16141 tcatgcaatg cagcaggccg cacctttctt tgccgatgat gaagacattt taatgctcta
    16201 cggcgacgtg ccgctgatct ctgtcgaaac actccagcgc ctgcgtgatg ctaaacccca
    16261 gggtggcatt ggtctgctga cggtgaaact ggatgatccg accggttatg gacgtatcac
    16321 ccgtgaaaat ggcaaagtta ccggcattgt tgagcacaaa gatgccaccg acgagcagcg
    16381 tcagattcag gagatcaaca ccggcattct gattgccaac ggcgcagata tgaaacgctg
    16441 gctggcgaag ctgaccaaca ataatgctca gggcgaatac tacatcaccg acattattgc
    16501 gctggcgtat caggaagggc gtgaaatcgt cgccgttcat ccgcaacgtt taagcgaagt
    16561 agaaggtgtg aataaccgcc tgcaactctc ccgtctggag cgcgtttacc agtccgaaca
    16621 ggctgaaaaa ctgctgttag caggcgttat gctgcgcgat ccggcgcgtt ttgatctgcg
    16681 cggtacgctt actcacgggc gcgatgttga aattgatact aacgttatca tcgagggcaa
    16741 cgtgactctc ggtcatcgcg tgaaaatcgg caccggttgc gtgattaaaa acagcgtgat
    16801 tggcgatgat tgcgaaatca gtccgtatac cgttgtggaa gacgcgaatc tggcagcggc
    16861 ctgtactatt ggcccgtttg cccgtctgcg tcctggtgct gagttgctgg aaggtgcaca
    16921 cgttggtaac ttcgttgaga tgaaaaaagc gcgtctgggt aaaggcacga aagccgggca
    16981 tctgacttac ctgggcgatg cggaaattgg cgataacgtt aacatcggcg cgggaaccat
    17041 tacctgcaac tacgatggtg cgaataaatt taagaccatt atcggcgacg atgtgtttgt
    17101 cggttccgac actcagctgg tggccccggt aacagtaggc aaaggcgcga ccattgctgc
    17161 gggtacaact gtgacgcgta atgtcggcga aaatgcatta gctatcagcc gtgtgccgca
    17221 gactcagaaa gaaggctggc gtcgtccggt aaagaaaaag taattctggc cgggtaatcc
    17281 ggtcatctgg gatgaggaga taacataatc tccctcccac aagcagtaac tataaaaata
    17341 accccactct ctacaaggct cggggcgccc gaaaaaacgg gcatacaggt tgaccgacaa
    17401 cgatataaat cggaatcaaa aactatgtgt ggaattgttg gcgcgatcgc gcaacgtgat
    17461 gtagcagaaa tccttcttga aggtttacgt cgtctggaat accgcggata tgactctgcc
    17521 ggtctggccg ttgttgatgc ggaaggtcat atgactcgcc tgcgtcgcct cggtaaagtc
    17581 cagatgctgg ctcaggcagc ggaagaacat cctctgcatg gcggcaccgg tattgctcac
    17641 actcgctggg cgacccacgg tgaaccttca gaagctaacg cgcatccgca tgtttctgaa
    17701 cacattgtgg tggtgcataa tggcatcatc gaaaaccatg aaccgctgcg tgaagagtta
    17761 aaagcgcgtg gctatacctt cgtttctgaa accgacaccg aagtgattgc ccatctggtg
    17821 aactgggagc tgaaacaagg cgggactctg cgtgaggctg ttctgcgtgc tatcccgcag
    17881 ctgcgcggtg cgtacggtac agtgatcatg gactcccgtc acccggatac cctgctagcg
    17941 gcacgttctg gtagtccgct ggtgattggc ctgggtatgg gcgaaaactt tatcgcttct
    18001 gaccagctgg cgctgttgcc ggtgacccgt cgctttatct tccttgaaga gggcgatatt
    18061 gcggaaatca ctcgccgttc ggtaaacatc ttcgataaaa ctggcgcgga agtaaaacgt
    18121 caggatatcg aatccaatct gcaatatgac gcgggcgata aaggtattta ccgtcactac
    18181 atgcagaaag agatctacga acagccgaac gcgataaaaa acacccttac cgggcgcatc
    18241 agccacggtc aggttgattt aagcgagctg ggaccgaacg ccgacgaact gctgtcgaag
    18301 gttgagcata ttcagatcct cgcctgtggt acttcttata actccggtat ggtttcccgc
    18361 tactggtttg aatcgctggc aggtattccg tgcgacgtcg aagtcgcctc tgaattccgc
    18421 tatcgtaaat ctgccgtgcg tcgtaacagc ctgatgatca ccttgtcaca gtctggcgaa
    18481 accgcggata ccctggcggg cctgcgtctg tcgaaagagc tgggttacct tggttcgctg
    18541 gcaatctgta acgttccggg ttcttctctg gtgcgcgaat ccgatctggc gctgatgacc
    18601 aacgcgggta cggaaatcgg cgtggcgtcc actaaagcat tcaccactca gttaactgtg
    18661 ctgttgatgc tggtggcgaa gctgtctcgc ctgaaaggtc tggatgcctc cattgaacat
    18721 gacattgtgc atggtctgca ggcgctgccg agccgtattg agcagatgct gtctcaggat
    18781 aaacgcattg aagctctggc agaagatttc tctgacaaac atcacgcgct gttcctgggc
    18841 cgtggcgatc agtacccaat cgcgctggaa ggcgcattga agctgaaaga gatctcttac
    18901 attcacgctg aagcctacgc tgcaggcgag ctgaaacacg gcccgctggc gctgattgac
    18961 gcggatatgc cggttatcgt cgttgcaccg aacaacgaac tgttggaaaa actgaaatcc
    19021 aacattgaag aagttcgcgc gcgtggcggt caattgtatg tcttcgccga tcaggatgcg
    19081 ggttttgtaa gtagcgataa catgcacatc atcgagatgc cgcatgtgga agaggtgatt
    19141 gcaccgatct tctacaccgt tccgctgcag ctgctggctt accatgtcgc gctgatcaaa
    19201 ggcaccgacg ttgaccagcc gcgtaacctg gcaaaatcgg ttacggttga gtaatcacta
    19261 tcatagataa aaaagggctg ccga
//
LOCUS       DAJTOW010000078        17357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTOW010000078 DAJTOW010000000
VERSION     DAJTOW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.078"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(31..762)
                     /gene="rlmB"
                     /locus_tag="OO244_002815"
     CDS             complement(31..762)
                     /gene="rlmB"
                     /locus_tag="OO244_002815"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5664571.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(942..3365)
                     /gene="rnr"
                     /locus_tag="OO244_002816"
     CDS             complement(942..3365)
                     /gene="rnr"
                     /locus_tag="OO244_002816"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5664572.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREVRIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATKAK
                     RAAKKKVAE"
     gene            complement(3404..3829)
                     /gene="nsrR"
                     /locus_tag="OO244_002817"
     CDS             complement(3404..3829)
                     /gene="nsrR"
                     /locus_tag="OO244_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5664573.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4034..5332)
                     /gene="purA"
                     /locus_tag="OO244_002818"
     CDS             complement(4034..5332)
                     /gene="purA"
                     /locus_tag="OO244_002818"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5664574.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5436..5633)
                     /locus_tag="OO244_002819"
     CDS             complement(5436..5633)
                     /locus_tag="OO244_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5664575.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5715..6719)
                     /gene="hflC"
                     /locus_tag="OO244_002820"
     CDS             complement(5715..6719)
                     /gene="hflC"
                     /locus_tag="OO244_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5664576.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6722..7981)
                     /gene="hflK"
                     /locus_tag="OO244_002821"
     CDS             complement(6722..7981)
                     /gene="hflK"
                     /locus_tag="OO244_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5664577.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8067..9347)
                     /gene="hflX"
                     /locus_tag="OO244_002822"
     CDS             complement(8067..9347)
                     /gene="hflX"
                     /locus_tag="OO244_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5664578.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9423..9731)
                     /gene="hfq"
                     /locus_tag="OO244_002823"
     CDS             complement(9423..9731)
                     /gene="hfq"
                     /locus_tag="OO244_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5664579.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9817..10767)
                     /gene="miaA"
                     /locus_tag="OO244_002824"
     CDS             complement(9817..10767)
                     /gene="miaA"
                     /locus_tag="OO244_002824"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5664580.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10760..12607)
                     /gene="mutL"
                     /locus_tag="OO244_002825"
     CDS             complement(10760..12607)
                     /gene="mutL"
                     /locus_tag="OO244_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5664581.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIQLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12617..13954)
                     /gene="amiB"
                     /locus_tag="OO244_002826"
     CDS             complement(12617..13954)
                     /gene="amiB"
                     /locus_tag="OO244_002826"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5664582.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13973..14434)
                     /gene="tsaE"
                     /locus_tag="OO244_002827"
     CDS             complement(13973..14434)
                     /gene="tsaE"
                     /locus_tag="OO244_002827"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5664583.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14406..15953)
                     /gene="nnr"
                     /locus_tag="OO244_002828"
     CDS             complement(14406..15953)
                     /gene="nnr"
                     /locus_tag="OO244_002828"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5664584.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15952..17091
                     /gene="queG"
                     /locus_tag="OO244_002829"
     CDS             15952..17091
                     /gene="queG"
                     /locus_tag="OO244_002829"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5664585.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17074..17127)
                     /locus_tag="OO244_002830"
     CDS             complement(17074..17127)
                     /locus_tag="OO244_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664586.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3937 a         4949 c         4361 g         4110 t
ORIGIN      
        1 atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg cttcaaataa
       61 gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct
      121 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
      181 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
      241 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt
      301 cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt
      361 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg cgtccgcact
      421 acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca gcaggaacgg
      481 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt
      541 cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg
      601 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
      661 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
      721 cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
      781 cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt
      841 gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt
      901 ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc
      961 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt
     1021 ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag
     1081 gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc gccttttttc
     1141 gctttctcgc gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta
     1201 aagtcgattt tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc
     1261 aggcgataag tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga
     1321 tagtagtcat tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga
     1381 cggacgaaga agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc
     1441 tggtcgagca tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca
     1501 cgacgttccg ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg
     1561 tagccgccgg tttcagtggt gttaccctga tgcccctgct ctttcgccag cagatattta
     1621 atggcgcggt gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag
     1681 gactgcaatg ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc
     1741 gagcgtagca gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc
     1801 agttccgcgt agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc
     1861 gccagcactg aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac
     1921 agtgccggtt ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg
     1981 cactcttcaa ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg
     2041 cgttcagcgt tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct
     2101 tcacgggctt tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc
     2161 ggggcgtact gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag
     2221 gtcagacgcg cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct
     2281 ttcgacgaaa ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa
     2341 cacaggccgt tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc
     2401 gacgtgccac ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg
     2461 acgtcggcaa tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact
     2521 gcatcgtcaa agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa
     2581 tcgacacggc ccgcttttgc ttcttccggc acttcttctt tcagccccgc aacctgttgc
     2641 tcaacagcct gcggccagat atacggaatt tcatgggtac gcagagcgat atcaaccgcc
     2701 atgccggtgc ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg
     2761 cgagtcggac gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc
     2821 tgatcgggcg ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg
     2881 acgcccgctt cggtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg
     2941 acaatacgca cttcacgacg acctttacgg tcagcaccca gcggctgcgc cagcacctga
     3001 tcgccatgaa tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct
     3061 tcaacccgca gaaagccgta gccatcacgg tgaccaataa cggtaccttt caccaggtcg
     3121 aggcgttccg gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc
     3181 atcgcgcgca ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct
     3241 accgccagct catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat
     3301 tcccgactag ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt
     3361 gacatctcgg ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata
     3421 atttataaag cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg
     3481 taagaaaact ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt
     3541 ggcaaaactc actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac
     3601 caatacgtat cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt ccacgaacag
     3661 cagtcacgta gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga
     3721 cgccgtagac gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca
     3781 tgtagatcag cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa
     3841 aagggaaatc gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc
     3901 taatttattt cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt
     3961 aatggcttaa ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc
     4021 aggcgtacca gaattacgcg tcgaacgggt cgcgcagaat catggtttca gtacgatccg
     4081 gaccggtaga gatgatatcg atcggcacac cggtcagctc ttcaatacgc ttgatgtagt
     4141 tcagcgccgc ctgcggcagg ccgctacgat ctttcacacc gaaagtggat tcagaccagc
     4201 ccggcatggt ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg
     4261 tagtcacttc gcgaccatcc ggcatacggt aagccacgca gagtttaacc tctttcaggc
     4321 catccagaac gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac
     4381 gacgaacggc aacggtgtcc agccagccgg tacgacgacg acgacccgta gttgcaccga
     4441 attcgttacc ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gccgggaaag
     4501 gacctgcacc tacacgagtt gagtaagctt tgaggatacc cagaacgtaa tcaacataac
     4561 gcgggcccag gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt
     4621 acggataagt accgtggtcg atatccagca gcgtaccctg cgcaccttcg aacatgacga
     4681 aatcgccacg ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca
     4741 ggatgtcggc aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt
     4801 tgtagtagtt aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga
     4861 aggtttcttt gtcgaaaagg tcgccaacac gcagaccgcg acgtgccact ttatcttcat
     4921 aagcaggccc gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac
     4981 gcgcgttatc cagagcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca
     5041 gacgctcacg aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg
     5101 ccggagacag cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg
     5161 atggaataag atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt
     5221 gaccgccctg gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac
     5281 ctttaccttc gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa
     5341 atcaccgttt gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg
     5401 cactcaaaaa tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg
     5461 tagtagacca caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg
     5521 ggcaaattgg tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct
     5581 aaaccttcca gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat
     5641 ccttatagaa aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta
     5701 ccgcagtcgt tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg
     5761 ctatccgggc tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc
     5821 aggctacgga tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg
     5881 gctgcttcgg catcgccttc accacgcata atgcggccct gacgctcagc ttctgccagc
     5941 gttctggtca cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt
     6001 gaacggtgac gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca
     6061 gacacttcgg tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc
     6121 gccgccatac tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc
     6181 tctgccgctt cggcaatggc gttatctgct gccggggtag taacttcatc ttctgtaccc
     6241 gcagaaccgg agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg
     6301 acgatatctt tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt
     6361 ttcaacagca cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg
     6421 aaatcgctga tgcgccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc
     6481 acaaagcggt cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt
     6541 tcaatgaacg gtatcttgaa atgcagacca ggctcataaa ccaaaggttt gttgtcatcg
     6601 tcacgcagta ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag
     6661 acagacatgt aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc
     6721 gttattcccc ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa
     6781 tatcgccctg actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg
     6841 gcggcagacg cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac
     6901 ctttcagcat ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca
     6961 ctttgcgggt gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa
     7021 tttccggcgc ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag
     7081 cttccaggat ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac
     7141 cgttcgcacg cggctgaact tcgttggtat acgcttctgc ttcacgaatg tattgctgtt
     7201 cgttttcacg cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag
     7261 cagcctggaa gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt
     7321 ccagttcgcg ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca
     7381 tggtgtattt accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat
     7441 ccgggctggt cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca
     7501 tctcaacgcg cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac
     7561 gcacggcttc cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac
     7621 ccggctcaac cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt
     7681 taatggtata gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa
     7741 cgacacgacc gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg
     7801 tgcctttacc gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta
     7861 aatcaggtgg cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag
     7921 gtttgctgct tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca
     7981 tgtttgtgct ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat
     8041 gactccacgc ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt
     8101 ttacagaggc gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc
     8161 tcctccatcc actctttttc tattgcctga agctgataaa aacgactcct cagacgccct
     8221 tcctgcggtg gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa
     8281 gcctgaaaaa gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt
     8341 ttgttctctt cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc
     8401 atcaccagca gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct
     8461 tcgatgtttt cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct
     8521 tgccgcgtct cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg caggtggcga
     8581 ataaacccta cggtatctgc aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc
     8641 aacgtcgggt cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg
     8701 cgattgaaaa gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg
     8761 tcggctttga tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgc
     8821 gactgtatct gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca
     8881 cccggaccac gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc
     8941 aggcgcgtag ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta
     9001 cgcgcacgtt gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg
     9061 cacaaacgct ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc
     9121 gaagcaccca tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa
     9181 tactttgggt gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg
     9241 gaagagacca gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag
     9301 atgtgtacca gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc
     9361 taaatagatc agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa
     9421 ccttattcgg tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga
     9481 tggtagttac tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagat
     9541 gggacaacag tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg
     9601 atcacgaact gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa
     9661 atagaaactg gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc
     9721 cccttagcca ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg
     9781 aaaaaattgc gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac
     9841 ctgtaatact tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc
     9901 ccaaccacgc agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg
     9961 ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc
    10021 cacgcaacga atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac
    10081 ttctgcttca aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag
    10141 cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc
    10201 tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg
    10261 ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg
    10321 aagttgacga tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg
    10381 cacttccggg tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa
    10441 catcgtaccg cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc
    10501 cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag
    10561 caatcggtgc ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat
    10621 ccctttgtaa ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag
    10681 ctcaatggct aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg
    10741 caggctcgcc ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa
    10801 cagattgtaa cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt
    10861 ccgccagcag ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc
    10921 gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta
    10981 tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga
    11041 tggtcacatg ctgtgcatct gactggaaat caatacccaa ttccgctagg gcagactgcg
    11101 ctttttctaa tgccgatttt tcttcgccag aaacttttag ccgcaactga atcagcagcg
    11161 gctgggcgca aacgggcgct tcacccggcg tcaattgtgc ctgacgcagc caacgttctg
    11221 ccactggcaa ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat
    11281 ggacgatagt cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg
    11341 gttccggcgc ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt
    11401 caccttgctg tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc
    11461 ctgaggcggg cgcaggagag tagcgcggag ctaccggctc acgagctgcc ggttctgcaa
    11521 agtgattgcg ccccgccgcc acgcggtttt ccgggatggc acgcggtgcg ggttgtggtt
    11581 catcgtccag cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat
    11641 agataaaatc atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta
    11701 cgttgacgtc cacctgatgc gggtcgatct ccagatacaa cacaaatgcc ggttgctgat
    11761 cggcccccag tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca
    11821 tcatgcgacc attcacgtag caatactgaa tttccgccag tgcgggcgta gtgtgatttg
    11881 gatcggccac ccagccgcgc agggtgagat cgccgtgttg ccattcaatc gccagcgctt
    11941 gttcgagaaa agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg
    12001 gcactgcgcg gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga
    12061 aacgcgccag cgcaatgcgg cggatgattt catcaatatg gctaaattcg gttttctcgg
    12121 tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg
    12181 ttcccaccgg atgcgctgcc ggtttaaccg tcacgtccat gtcgcgtcct tcggcatagg
    12241 cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac
    12301 tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca
    12361 gagaggcgat tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga
    12421 taccgcagcc gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat
    12481 cgatacgcgt cgcacctgca tcgaggctgt tttccactag ttctttgact accgacgcag
    12541 gtcgctcgac cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa
    12601 ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag
    12661 tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaag taattgcgca
    12721 ggcctttgta aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt
    12781 cttcgctgtt gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgagc
    12841 gcagaacgcc aaggctggcg tgttctggtc gacgtttatg tatttcgcca atgcgttgca
    12901 actgactgat catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta
    12961 aatccagcac cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg
    13021 ccccacccag cagctccgac agtttctcgt gctgctccag ccagctggcc atttcactgt
    13081 tggcgcgacg gttagagagc acccatacgg aagcgccagt cgcactacgg ttcggtgcgg
    13141 catcagcgtg aatcgacacg aggaaattgg cgttttgctt atgtgccaca tcactgcgcc
    13201 ccatcaccga gataaagtaa tccccgtcac gggttaaaac gcctttaaac atcggatcgt
    13261 cattgagcaa agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac
    13321 caccggggcc gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa
    13381 ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga
    13441 taacacccgt agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc
    13501 gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg
    13561 gtggcggcac atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct
    13621 tcaccgcttc ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat
    13681 ctttaggcgt tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca
    13741 gtccctgaat catgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc
    13801 taaacgcata atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt
    13861 tagaaacctg aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg
    13921 tcgctaccaa ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg
    13981 ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc
    14041 cttgtgcctg ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt
    14101 gttgtggcca ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa
    14161 actccagctc ctcgggatcg gcaaggcggt acaaatcaaa gtgatagacc attaagttgt
    14221 cgagcgtata gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca
    14281 gagcctgtaa aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat
    14341 agattacggt tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct
    14401 gctcatcagg gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta
    14461 acaatacgct gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc
    14521 gccgccagta cgtcagctgc cgcaccgtgc gcgacacagc ctgcacaggc tgcatcatac
    14581 ggcgacagtt tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg
    14641 ccgctcgcca tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg
    14701 accacggttc cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg
    14761 gcgcaatgta agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc
    14821 tcgctaggat gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg
    14881 ttcaatgcat cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct
    14941 tttttccccc actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc
    15001 aggctttcgg taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc
    15061 agcagcggcg caatgttctc actgcgggtc agtactcgga ctaaaccagc accagcacgt
    15121 agcgccgctt cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatc
    15181 accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa
    15241 agttgttctg ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc
    15301 cccagtgagt caaaatgcag ttgtcctgta acatcccgcg cttttccagt gagcaagcct
    15361 agtttcagcg caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg
    15421 ccggtttcag ccagcaggcc ggaagggata tcaaccgcca caatcggcgc aggatgggaa
    15481 ttagcgtggt cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtaccg
    15541 agcagcgcat caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc
    15601 tcgccacccg cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg
    15661 tcgctctcct gggccaacaa cgtgacctca atgccgaccg ctttggccag tcgcgcgacc
    15721 acgtagccat cgccgccgtt attaccatga ccgcacagca ccagccagtg gcgggcgtca
    15781 ggatacgccg aacgacacac ctggaatgcg gcctcgccag cgcgaagcat cagctcatag
    15841 agtgtgagcc ccagcacatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac
    15901 cagacggtgt gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag
    15961 cccctcgatc tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt
    16021 cagcaggtag gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg
    16081 ctggacaaac aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct
    16141 cgcccccatg agttattacc cggtacgctg cgtgtaatca gcgtgcggat gaattacctt
    16201 cctgctaacg ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt
    16261 tatgcgctgg gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag
    16321 atgattcagc aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt
    16381 ctcgagcgcc cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc
    16441 ctcaatcgcg aggccggttc gttcttcttt ttaggcgaat tgctggtcga tattccgctg
    16501 cccgtggatc aaccagtcga ggaaggatgc ggcaaatgcg tggcctgtat gacgatttgc
    16561 ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc
    16621 accatcgaac ttgaaggggc gctcccggaa gagttgcgac cgttaatggg aaaccgtatt
    16681 tacggttgcg atgactgcca gcttatctgc ccgtggaatc gctattcgca actcactaca
    16741 gaagacgatt tcagcccgcg taagccgcta cacgcaccgg aactcattga gttatttgcc
    16801 tggagcgaag agaagttttt aaaagtcacg gaaggatcgg cgattcgccg tataggtcac
    16861 ctgcgctggc tgcgtaatat cgccgtggca ttaggcaatg ccccttggga tgaaacgatt
    16921 ttgacagcgc tggaaagtcg taaaggtgag cacccacttc tcgatgagca catagcgtgg
    16981 gcgattgcgc agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa
    17041 aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac
    17101 agcttgtgaa taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat
    17161 cgactcgaca aaacggaatc ttgattttta acttatttat caataggtta aaaaaatacc
    17221 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
    17281 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtagat
    17341 attttattga gagaaga
//
LOCUS       DAJTOW010000079        17334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTOW010000079 DAJTOW010000000
VERSION     DAJTOW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.079"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..427)
                     /gene="chbR"
                     /locus_tag="OO244_002831"
     CDS             complement(<1..427)
                     /gene="chbR"
                     /locus_tag="OO244_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5664587.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETG"
     gene            complement(435..785)
                     /gene="chbA"
                     /locus_tag="OO244_002832"
     CDS             complement(435..785)
                     /gene="chbA"
                     /locus_tag="OO244_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5664588.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(844..2193)
                     /gene="chbC"
                     /locus_tag="OO244_002833"
     CDS             complement(844..2193)
                     /gene="chbC"
                     /locus_tag="OO244_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5664589.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2278..2598)
                     /gene="chbB"
                     /locus_tag="OO244_002834"
     CDS             complement(2278..2598)
                     /gene="chbB"
                     /locus_tag="OO244_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5664590.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AVAN"
     gene            complement(2897..3235)
                     /gene="osmE"
                     /locus_tag="OO244_002835"
     CDS             complement(2897..3235)
                     /gene="osmE"
                     /locus_tag="OO244_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5664591.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3437..4264
                     /gene="nadE"
                     /locus_tag="OO244_002836"
     CDS             3437..4264
                     /gene="nadE"
                     /locus_tag="OO244_002836"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5664592.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PNRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4494..5381
                     /gene="cho"
                     /locus_tag="OO244_002837"
     CDS             4494..5381
                     /gene="cho"
                     /locus_tag="OO244_002837"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5664593.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5341..5916)
                     /gene="ves"
                     /locus_tag="OO244_002838"
     CDS             complement(5341..5916)
                     /gene="ves"
                     /locus_tag="OO244_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5664594.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6119..6604)
                     /gene="spy"
                     /locus_tag="OO244_002839"
     CDS             complement(6119..6604)
                     /gene="spy"
                     /locus_tag="OO244_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5664595.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6934..7902)
                     /gene="astE"
                     /locus_tag="OO244_002840"
     CDS             complement(6934..7902)
                     /gene="astE"
                     /locus_tag="OO244_002840"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5664596.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7895..9228)
                     /gene="astB"
                     /locus_tag="OO244_002841"
                     /pseudo
     CDS             complement(7895..9228)
                     /gene="astB"
                     /locus_tag="OO244_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9225..10703)
                     /gene="astD"
                     /locus_tag="OO244_002842"
     CDS             complement(9225..10703)
                     /gene="astD"
                     /locus_tag="OO244_002842"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5664597.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10700..11734)
                     /gene="astA"
                     /locus_tag="OO244_002843"
     CDS             complement(10700..11734)
                     /gene="astA"
                     /locus_tag="OO244_002843"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5664598.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11731..12951)
                     /gene="astC"
                     /locus_tag="OO244_002844"
                     /pseudo
     CDS             complement(11731..12951)
                     /gene="astC"
                     /locus_tag="OO244_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13397..14203
                     /gene="xthA"
                     /locus_tag="OO244_002845"
     CDS             13397..14203
                     /gene="xthA"
                     /locus_tag="OO244_002845"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5664599.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14370..15080
                     /locus_tag="OO244_002846"
     CDS             14370..15080
                     /locus_tag="OO244_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5664600.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15085..15762
                     /gene="ydjY"
                     /locus_tag="OO244_002847"
     CDS             15085..15762
                     /gene="ydjY"
                     /locus_tag="OO244_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5664601.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15777..16484
                     /locus_tag="OO244_002848"
     CDS             15777..16484
                     /locus_tag="OO244_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5664602.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16484..17032
                     /locus_tag="OO244_002849"
     CDS             16484..17032
                     /locus_tag="OO244_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5664603.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17042..>17334
                     /locus_tag="OO244_002850"
     CDS             17042..>17334
                     /locus_tag="OO244_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5664604.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLL"
BASE COUNT         4280 a         4608 c         4253 g         4193 t
ORIGIN      
        1 gccctgtttc gcggaaattt aatgaagaaa tcactgtttc cacataggtg agaaaagcgt
       61 tattggtccg gtatacctgc gaagcaacaa agcaataagg caacaacggc aggtaatgct
      121 gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg cgtggcacca aactcataaa
      181 aactttgatg gtgcgaacct aacggaataa aaacaaaatc gccccgttcc agtaacacgc
      241 gcttaccgtt aatctcctgg aaataacgcc cggttaatac cagagtaaat tcataatagt
      301 cgtgctgatg cagtccgctg atactctcag ttttgttata gataaacaca tggaagtttt
      361 tgccattaaa caactgctgt tctcgggcag tggcaatttc cggcgcgtta atcactggct
      421 gcatcatcga ctccttatgc cttcagtttt tcatgaagct caattaattc agtaatcagt
      481 tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt ggaccagcac cagactcact
      541 ttcatcttac cttcgcccgc atcgccttca atcagtttcg tctgtaccag atgcgcttca
      601 ttcaatgcca tccgtgactg atccatcatg gctttcgctg cggcaaaatc gccctgcttc
      661 gcctgtttca atgcggcata cgccaggcta cgcgcttgtc cagagttgat gataagcccc
      721 atcaccactt cttccagctc ttcagcttct gtttgcgtat caggaatatt atcgagatcc
      781 atcatacatc gttcctctct cttcttaccg gcaagattac ccgtaccggc atcgattaaa
      841 atttcagggg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
      901 gttagccacc acaacaaagg gcagataaat taacgttgcg atgccaaggt tgaagagtgc
      961 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     1021 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     1081 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     1141 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     1201 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     1261 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     1321 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     1381 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     1441 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     1501 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     1561 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     1621 aattaatgcc gagaaagaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     1681 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accagtccaa taataatccc
     1741 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     1801 atatggggtg acggtcataa atgctgcaac ggataacaac ccagccgcca gcgcatcgac
     1861 tttacgctct tctgccagcg ccatgccaat aaagaacggt gccattaaag acattattcc
     1921 taatgttccg ttatatacgt tgccgccaat acctttcaga ccattaagtg tttcaatggt
     1981 tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgac ccctccccaa agcttagaaa
     2041 aacgttgtta attaatacaa acatggcccc cgcaagggtt aacggcatta agcgaataaa
     2101 gccatttttg attgcattaa cgtgtggctg ctttcctatt ttaactgcaa aagggaggag
     2161 taccttttca agcgatgcaa taacattact catagaaaaa tacccttaaa aaccgcaatt
     2221 taaatattac ggtattgatt tatgaaataa ctctttgacg ggaaaattta aaataaatta
     2281 atttgctacg gcttttttaa tcgctgcaac cgcagcctta agcacgccta aaccatcgac
     2341 tttgccataa agcagcgagt caattacttc aaccggtttg ttgggtaaca aacgctggat
     2401 ttcgggcaac atataagcaa tctgcggccc taataacacg acatcggcat tctgaccttt
     2461 ttcaccagcc agtgtttccg gaaatgcttc aataatgacc ggaacttcat atttttctgc
     2521 ctgtgcgcgc atttttgata ccagtaaaga gatagacatg cccgcagaac aaaacagata
     2581 aatgtgtttc ttttccataa aactgccctc atcgacgatt atctgtcagc cagacactcc
     2641 gcaagccttg acctgcttcc atgctctggg taacttgcga aaccaacatg actttttttg
     2701 tggctgaaag gagtatacgg tatagacctg agcaatagta tttctttgac cttataaatt
     2761 gtttctcctt gacctgtggt tatgaccctc ttcacatttc gggcaaataa ttcgcgaaga
     2821 taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc ggtttccgtt gttactcaag
     2881 ctttcagacg aattgattac ttcgcagcct gtggatcagt gtcgtattca gcacaggtct
     2941 gataaccgga gttgatgaca tgtccggtat catctaacgc gacaaagtag gtttctgctt
     3001 taccatcacg ttgacccagg atgtaggtct ggcaagtacc gcgagcatgg atcatgctca
     3061 cttcagacga aggtttaccc gcaatttgtg caacctgcgc ccggctcatg ccttttttga
     3121 cgtctttcac cacaggctgt acaaactggt ctttggtacg atcataagct gtacaacccg
     3181 ccagcatggt taataccgcc gctgcactca gaattcctgc catattcttg ttcatattcc
     3241 gtcctcttgt ttatcagcgt gttagataag cctggaatac attgggcgct ttttcaagcc
     3301 cgtgaacgaa acggctccgc tttcagagga ttcctgtatg acgttttaac caccattcag
     3361 cccgctgtcg cttgtcgttt cagtagcaac gggttagctt taaggaagtt ttgtcttttc
     3421 tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg gcgcaaaacc
     3481 gcagattaat gctgaagagg aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca
     3541 aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg actccacgct
     3601 tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc caggaaactc gcaacgaatc
     3661 actgcaattt attgccgtac gcctgcccta tggtgttcag gctgacgaac aagattgcca
     3721 ggatgccatt gcctttattc aaccgaatcg cgtattaacc gttaatatca agggcgcggt
     3781 attggctagc gagcaggcat tgcgggaagc aggcattgaa ctgagcgatt ttgtccgtgg
     3841 caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta tgaccagcgg
     3901 tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca ctaaatatgg
     3961 tgacggcggt acggatatta atccgctgta tcgtctcaac aaacgccagg gtaaacagtt
     4021 actgacggca ttaggttgcc cggaacacct ttataagaaa gccccaactg ccgatcttga
     4081 agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtgacctatg acaatatcga
     4141 cgactatctg gaagggaaaa acctacctga acaggtcgcc agaacaatag agaactggta
     4201 tctgaaaacc gaacataaac gccgtccgcc aattaacgtt ttcgatgatt tctggaaaaa
     4261 gtaataattt gcacctattg gattgtgcga aaaagagtaa tttgttcacg ccggatgcgg
     4321 cgtgaacgcc ttattctacc tacaaaacta tgcaaattca atacattgca ggagtcgaat
     4381 aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc gcctcttttt
     4441 taatcgcctc ccgcctgtta cactggatag ataaccagca ttcggagtca acagtggtac
     4501 ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa tatcccgaac
     4561 atttacattc attccttaat gacttaccca cccgaccagg ggtgtatctg tttcatggcg
     4621 aaagtgacac catgccgctc tatatcggca aaagcgttaa tatccgcagt cgggtgcttt
     4681 ctcatttacg caccccggat gaagccgcca tgctacggca atcccggcga attagctgga
     4741 tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc aaagaacaac
     4801 agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgtgccctg caattaaatg
     4861 aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc cccaacctgt
     4921 ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc gctgatgaac
     4981 aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt gcatgttttc
     5041 gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat gaggaacatg
     5101 cgctacgctt gcgccagtct ctggagcgtt tgcgagtggt gtgttggcct tggcaaggtg
     5161 cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt caaaactggc
     5221 tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg acacccgccg
     5281 ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt aactatgaaa
     5341 ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga acaacagttt
     5401 accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcaca cgccctggtc
     5461 ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta atcacaaaca ccaccccgcc
     5521 gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct tacagacatc
     5581 caggcgcgtc ataatattga aatccatcga catctgccct gccgtcagtt tcgcttttac
     5641 cacctggtcc gccgtaaagg caaaaggctg taacggtttt aaggtatggt taaagcggtc
     5701 tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt ccatgccggg
     5761 aaacaaagaa aactccccat tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc
     5821 acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag
     5881 attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc cgttcttttc
     5941 attgttatcg ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt tacgttagtg
     6001 gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg atcctgtcca
     6061 ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaagatt
     6121 attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa
     6181 aattagcatt aaattgcttt ttctgttccg gcgtcaggat gttgtaaatt ttgttctggg
     6241 tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg atctgcgctt
     6301 cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg cggcgttctt
     6361 ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgtgg atctgctgtt
     6421 tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga ccgaacttgc
     6481 ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat
     6541 gggccaggtt agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac
     6601 gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg
     6661 ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg
     6721 aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt
     6781 gatgagagaa tattacaaca cgatgatttt gcagagatta tgaagaacta taccggatga
     6841 ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact
     6901 taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc
     6961 cgcgcgtaat cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt
     7021 tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa
     7081 attcagcgtg tcacttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga
     7141 aaccacccga taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag
     7201 cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa
     7261 cgctttgcca agttccaacg tacaggccag cgcgccaaaa tgtctgacgc tgaaatgggt
     7321 aaacgtacca ccaggctcct gatggaacac cagcgcctcc agccccgccg cacccagcca
     7381 cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg
     7441 caaggagcca cgaattgcgg tatgtagatc aaggtgccaa cgcacagatt ctttgccctg
     7501 gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc
     7561 agcaaatagc tgccaacgac cgccaaacat tcggttcata tcgctatggc aataacgttt
     7621 cccttgcttc agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat
     7681 ctcgccgtga gatatcgcgc caagcaacgc gtccagcatt tccacaggtg ccgtctcatt
     7741 accgtggatc cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc
     7801 cagcacacca tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac
     7861 cggtttttta cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct
     7921 ggaacggata aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc
     7981 cttcgcgcag caattgcggg tcggccagat cggcagcagt aagacgatcg cggtagtaac
     8041 gatccaccca gtcattgagc gcattaaaca aggtatcgtt catcatcacc gccggattca
     8101 ccgcccggcg ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc
     8161 cattcgccat gctttcacgt aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg
     8221 caaggagttc attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca
     8281 ccaacatcat ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca
     8341 ccgcatccga cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc
     8401 gcgtgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg
     8461 gttactcacg gcaatcacgt cattatgaaa aacgctctgg tcgataacgt ccgggttttg
     8521 ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc
     8581 ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc
     8641 gtagacaaaa agtagcatac ccggttcacc gtaatgaccg ccgagacgat tgtggtttgc
     8701 cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc
     8761 ttcgtcctta aaaatcgctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg
     8821 aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga
     8881 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggaactga cgctggaaag
     8941 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     9001 caactgacgc agcaccggaa taaacggacg ctcgtgcggc ggaatcacgg cctgggggaa
     9061 tcccgcatcg gcaagggctt tcattttcag taagccctgt tttgccgcca gtcgcgggtt
     9121 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc ccgcgtaatg
     9181 atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca
     9241 tcggaaaaat ccagcccggg gttaagcgtg gcgggcaatg ttaacgagtc cgactccagg
     9301 ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg
     9361 gaagcaccaa tgccgccgaa tggggcggta ctggcagcac cagtaagcgg tttgttccag
     9421 ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg ctcgggggaa
     9481 accagaccgc aaaagagtcc gaagcgagtg ttgttcgcca tccgaatcgc ctcatcaaaa
     9541 gtatcataac gccagacgcg caaaaacggc ccgaacacct cttcatccgg cacaccgcca
     9601 acgcccgtca tttcaatgat ccctggtgtc agcaacgatg tccctgcttg taataagcgc
     9661 ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc
     9721 tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg
     9781 cccggcgtta atcgctggct gacggcaacc agactagcaa gaaacgcatc gccctgcgct
     9841 ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc
     9901 gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga
     9961 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc
    10021 agctggtagc cggtattggc gctaccggta aacagcaaac cgtcgagatc ctccagcgca
    10081 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag
    10141 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
    10201 ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga
    10261 ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc
    10321 gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt
    10381 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcggctta
    10441 cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca
    10501 aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc
    10561 gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat
    10621 aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag
    10681 tcaccgttaa tccataaagt catgctgttt tctcctctgc gcacaggcgc accagacgaa
    10741 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
    10801 cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt tcattggcga
    10861 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac
    10921 ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga
    10981 tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg
    11041 tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga
    11101 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
    11161 cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
    11221 agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc
    11281 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
    11341 gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa
    11401 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc
    11461 cgacgcgata gttgtaccag ggatcgttca gcccaaccgc cacctcaata gcgcaaatcc
    11521 ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg
    11581 gcagttcacc ttgccaggtt ttgattgccc tttcgatacg cgccgaaagc gtggcttcat
    11641 tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
    11701 catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaagtgttc
    11761 gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc
    11821 aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc atcacgcctg ctttcgccgc
    11881 ttccagagag atctgtttcg cttgcccggc gtaatcggca ttcagtacac agccaatcaa
    11941 caaacctaag ccgcgaactt cactgaacaa accatagcgg tgattaatag tattaagacg
    12001 ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag
    12061 ctccagcact ttgcctgcta ccgccgaggc cagcggatta ccgccatagg tggtgccatg
    12121 agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc aacaacgcac cgaccgggaa
    12181 accgccgccc agcgctttgg cggtggttaa cagatcgggc gtcacgccgt agtgcatata
    12241 ggcatacagc tcaccagtgc gcccgacgcc ggtttgtacc tcatcaaaaa tcagcaaggc
    12301 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac
    12361 accgccttcg ccctagatgg gctcgacaat caccgcacag gtggcgtcgt caatcagcgc
    12421 gctggcagag ttaatatcgt tatatgcagc atgacgaata tccgccggca gtggcgcaaa
    12481 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    12541 cgcatttttg aacgccacga tgccactctt atggctgccg tagcggtcgt gagcgaattt
    12601 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    12661 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    12721 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg
    12781 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    12841 ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta
    12901 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    12961 tacagtaaat aattcgttat ttgtatgtta acaataagta atgtttgcgc tgtaaatgta
    13021 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    13081 atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc
    13141 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    13201 agaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc
    13261 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    13321 cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga
    13381 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    13441 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    13501 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    13561 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc
    13621 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga
    13681 aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag
    13741 ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta
    13801 cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag
    13861 ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg
    13921 taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga gctggggggt
    13981 ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta
    14041 ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca
    14101 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    14161 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg
    14221 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt
    14281 gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta
    14341 ttatcaagtt tgatatagga aactccacga tgaacgctga gcgtaacttt ctgtttgcct
    14401 gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca
    14461 ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt tatagcctct
    14521 atattctgtt attcatcaat gcccccctct ttctgttacc aggaagcata ctggtgatcg
    14581 ctggaggaat agtttttggc ccgctcttag ggacacaact ctcattaatt gccgcaacgc
    14641 tggcctcctc gtgctcattc ctgctggcgc gctggctggg acgtgattta ctgctgaaat
    14701 acgtgggcca tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg
    14761 attttcttat tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt
    14821 acggattcac caccatcgcc ttctggcctt atacccttat ttcggcactc acgaccctgc
    14881 ccggtattgt tatttatacc gtgatggcaa gcgatctcgc caatgaaggc attacgctgc
    14941 gctttatttt acaactctgt ctggcgggcc tggcgctgtt tattctcatc cagctcgcaa
    15001 aactctacgc ccgacacaaa catgtggatc tgtctgcttc gcgccgcagc ccacttactc
    15061 acccaaaaaa tgaaggatag aacgatgtcg caacattatt cagtgtcatg gaaaaaagga
    15121 ctggctgcac tctgcttact ggctgttgcc gggcttagcg gctgcgatca aaaagagaat
    15181 gcggcggcaa aagtggaata cgacggactt tcgaacagcc aaccgctgcg cgtcgatgcc
    15241 aataaccata cggtaaccat gctggtgcaa attaatggtc gtttcctcac cgacgacact
    15301 cgtcacggta ttgtgtttaa agatggctcc aacggacata aatcgctgtt tatggcttat
    15361 gcgcccccga aagcatttta tgaagccctg aaagaggcag gtggtacgcc gggcgaaaac
    15421 atgacgatgg ataataaaga aacgactcat gtcacaggca gcaaactgga tatttcggtc
    15481 aactggcaag gggcggcaaa agcgtattcc ttcgatgaag tgattgttga cagtaatggc
    15541 aagaaactgg acatgcgctt tggcggtaat ttaacggcag cagaagagaa gaaaacgggt
    15601 tgcctggtgt gtctggatag ctgcccggtc ggcatcgtca gcaatgcaac atacacttat
    15661 ggtgcggtgg aaaaacacgg cgaagtgaaa ttcaaaggca atgcctcagt tctcccggcg
    15721 gataacacgc tggcaacggt tacctttaaa atcaccgaat aaagccaaga taaaggatga
    15781 tgaagataca atcgcgtaaa atctggtact accgtataac cctcatcatc ctgttgttcg
    15841 ctatgctgct cgcatgggcg ctgcttcccg gcgtccatga gtttatcaat cgcagcgttg
    15901 cggcgtttgc cgccgtggac caacagggta tagaacgctt tattcagtct tacggcgcac
    15961 tggcggcagt tgtctcgttc ttgttgatga ttttacaggc cattgctgca ccgctacctg
    16021 cgtttttgat cacctttgcc aatgcgtcgc tgtttggcgc gttctggggc ggcttgctgt
    16081 cgtggaccag ttcgatggcc ggcgcggcgc tgtgcttttt tatcgccaga gtgatgggcc
    16141 gcgaagtggt ggaaaaatta accggcaaaa ccgtgcttga cagtatggac ggctttttca
    16201 ctcgctacgg caaacacacc atcctggtct gtcggttatt gccttttgtc cctttcgatc
    16261 caatcagcta tgctgccggt ctgacttcaa tacgttttcg ctcgtttttt atcgccaccg
    16321 ggcttggtca attaccggcg actattgttt attcctgggc aggcagcatg ttaacaggcg
    16381 gtactttctg gtttgtcacc gaactgttta ttctgtttgc cctgaccgtg gtgattttta
    16441 tggcgaagaa aatatggctt gagcgccaga agaggaatga ctgatgggtt taccgccgct
    16501 tagcaaaatt cctttcattt tacgtccgca ggcgtggctg catcgtcgcc attacggcga
    16561 ggtgctaagc ccgattcgct ggtgggggcg gatcccgttt atcttttatt tggtgtcaat
    16621 gtttgttggg tggctggagc gcaaacgctc accgctcgat ccggtagtgc gatcgcttgt
    16681 cagcgcgcgc attgcgcaaa tgtgcctgtg tgagttttgc gtggatatca ccagtatgaa
    16741 agtcgccgag cgtaccggca gcaccgataa actgctggca gtggctgact ggcggcaaag
    16801 cccgctcttt agcgatgaag aacggctggc gctggagtac gccgaagccg caagcgtcac
    16861 accgccaaca gtcgatgatg ccctgcgtac ccgactggct gcgcattttg acgctcaggc
    16921 gctcaccgaa ctgacggcat tgatcggcct gcaaaacctg tcggctcgtt ttaattctgc
    16981 catggacatt cccgctcagg ggctgtgccg tattccggaa aaacgttctt aaggagggaa
    17041 gatgcgccat tgtgggtggt tgctgggatt gttatcgctg ttttctttgg caacacatgc
    17101 cagtgactgg caggaaatta aaaatgaggc caaagggcaa accgtctggt ttaacgcctg
    17161 gggcggcgat accgcaatta accgctatct cgactgggtt agcggcgaga tgaaaaccca
    17221 ttacgctata aacctgaaga ttgttcgtct cgcggatgcc gcagacgcgg tgaagcgcat
    17281 tcagaccgaa gctgctgccg gtcgtaaaac gggcggctcg gtggatctgc tctg
//
LOCUS       DAJTOW010000080        17018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTOW010000080 DAJTOW010000000
VERSION     DAJTOW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.080"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..438
                     /locus_tag="OO244_002851"
     CDS             <1..438
                     /locus_tag="OO244_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5664605.1"
                     /translation="PNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIVDGFHRH
                     EIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQL
                     GWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(439..1599)
                     /gene="ybdL"
                     /locus_tag="OO244_002852"
     CDS             complement(439..1599)
                     /gene="ybdL"
                     /locus_tag="OO244_002852"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5664606.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1708..2796
                     /gene="hcxA"
                     /locus_tag="OO244_002853"
     CDS             1708..2796
                     /gene="hcxA"
                     /locus_tag="OO244_002853"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5664607.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2806..3003)
                     /locus_tag="OO244_002854"
     CDS             complement(2806..3003)
                     /locus_tag="OO244_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5664608.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3185..5290)
                     /gene="cstA"
                     /locus_tag="OO244_002855"
     CDS             complement(3185..5290)
                     /gene="cstA"
                     /locus_tag="OO244_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5664609.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5471..5884)
                     /gene="entH"
                     /locus_tag="OO244_002856"
     CDS             complement(5471..5884)
                     /gene="entH"
                     /locus_tag="OO244_002856"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5664610.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5887..6633)
                     /gene="entA"
                     /locus_tag="OO244_002857"
     CDS             complement(5887..6633)
                     /gene="entA"
                     /locus_tag="OO244_002857"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5664611.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6633..7490)
                     /gene="entB"
                     /locus_tag="OO244_002858"
     CDS             complement(6633..7490)
                     /gene="entB"
                     /locus_tag="OO244_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5664612.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7504..9114)
                     /gene="entE"
                     /locus_tag="OO244_002859"
     CDS             complement(7504..9114)
                     /gene="entE"
                     /locus_tag="OO244_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5664613.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9124..10299)
                     /gene="entC"
                     /locus_tag="OO244_002860"
                     /pseudo
     CDS             complement(9124..10299)
                     /gene="entC"
                     /locus_tag="OO244_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10591..11547
                     /gene="fepB"
                     /locus_tag="OO244_002861"
     CDS             10591..11547
                     /gene="fepB"
                     /locus_tag="OO244_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5664614.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11551..12783)
                     /gene="entS"
                     /locus_tag="OO244_002862"
     CDS             complement(11551..12783)
                     /gene="entS"
                     /locus_tag="OO244_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5664615.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIDAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12894..13898
                     /gene="fepD"
                     /locus_tag="OO244_002863"
     CDS             12894..13898
                     /gene="fepD"
                     /locus_tag="OO244_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5664616.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13895..14887
                     /gene="fepG"
                     /locus_tag="OO244_002864"
     CDS             13895..14887
                     /gene="fepG"
                     /locus_tag="OO244_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5664617.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14884..15699
                     /gene="fepC"
                     /locus_tag="OO244_002865"
     CDS             14884..15699
                     /gene="fepC"
                     /locus_tag="OO244_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5664618.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15696..16829)
                     /gene="fepE"
                     /locus_tag="OO244_002866"
                     /pseudo
     CDS             complement(15696..16829)
                     /gene="fepE"
                     /locus_tag="OO244_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3969 a         4853 c         4430 g         3766 t
ORIGIN      
        1 ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga aatagatggt
       61 tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat cgtggatggt
      121 tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt aaaaggttat
      181 ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat
      241 aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg tgaacttagc
      301 cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg
      361 ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca atattcccgc
      421 gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca gcaacgtcga
      481 ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac
      541 cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa actcaacatc
      601 atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt cacacggtaa
      661 aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct ggcgataaaa
      721 gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa gcgccagctg
      781 tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt cggcgctgat
      841 gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg tcttgccaaa
      901 tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac tggcatggcc
      961 ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc
     1021 ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg cactggggtt
     1081 atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg caaattcctg
     1141 ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgc ttcactattc ccccagaaag
     1201 cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt catcgccatt
     1261 gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg ttacggtgat
     1321 atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag caatcgcctc
     1381 gcgcaaagcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg caacgtggta
     1441 cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt gcgacaggtt
     1501 aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg tgccaagttg
     1561 tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac ctgacgaagt
     1621 tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg tttagatgtc
     1681 taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc
     1741 ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt tttcactgat
     1801 gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc ggcgcaaacc
     1861 aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg tggtcattgc
     1921 agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag cgtggtgatt
     1981 ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg tctcggtctg
     2041 ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc gctctccgtc
     2101 tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc caattttatg
     2161 gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct ggcggggatc
     2221 ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg
     2281 ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt cttgttaaac
     2341 agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc attttgcgat
     2401 gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga tcgttttacg
     2461 cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca aaccgagaag
     2521 tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt gctgggtcag
     2581 gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc gactacgctg
     2641 gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat tgcccacacc
     2701 ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga tacgttgcgt
     2761 gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc agcagcgcgc
     2821 gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct cgtaggtcat
     2881 cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt aatcaggcat
     2941 accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa
     3001 catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt gaacgcttta
     3061 tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc ggggagaggg
     3121 ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc ccctcactct
     3181 gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc ggcatcggct
     3241 catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt gccgtcttaa
     3301 tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac ccggcatcca
     3361 gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac ggaatattgc
     3421 cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact ttcgcatccg
     3481 ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag gccgttggta
     3541 ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg gcacagagca
     3601 tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac agagtgttaa
     3661 taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc agtacgcaca
     3721 gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca ggagacacca
     3781 cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct accgccgtca
     3841 gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca tccatcatac
     3901 cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc gcaccgcccg
     3961 cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc gtatctggtg
     4021 taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca tccgccgtcc
     4081 ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc ggatcgatga
     4141 tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc ccaccgtagc
     4201 cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg gaagagatca
     4261 gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac gggaacaggt
     4321 taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc atggtcagcg
     4381 tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt ttcaggaagg
     4441 tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc gccacaaaac
     4501 cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac ggtgcccagg
     4561 tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac accaggccaa
     4621 tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt cccataaaaa
     4681 tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga gtcagggctt
     4741 tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag caggctacca
     4801 gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc agcgagcgac
     4861 cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg gcgagaacca
     4921 ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc agcaccggcc
     4981 ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc actttcttgt
     5041 ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata cgcgtcgggt
     5101 caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg atcagataga
     5161 tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga tttaaagcaa
     5221 tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg tatttccctg
     5281 atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt tgtttctata
     5341 acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa accaatattt
     5401 aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat ctcatttaat
     5461 tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa cgccgcccct
     5521 gttcatcgaa aacgacgatt tcccaactct gattttgccg cccaagatgc agcggctggc
     5581 agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta agttccgtgc
     5641 ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc agcgtttcag
     5701 ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta cgggtatcaa
     5761 ccggcatttc ggcttccagc acatcatctc ccagacgggt atacacaatt cccagatgcg
     5821 ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga
     5881 tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta gggtgatatg
     5941 gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat
     6001 tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc
     6061 ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc caggcgaaac
     6121 cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg ccaggctttt
     6181 cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt gtgcggcgtc
     6241 ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca tggtttgctg
     6301 gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt cctctttact
     6361 gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca cgtccagtcg
     6421 ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt cggcaacatc
     6481 cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa aacctgtaac
     6541 tttcgctccc gcctcaacaa acgccagcgc cgtggcgtag ccgatacctt tacctgcgcc
     6601 ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga gagtagcttc
     6661 caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc accatgcact
     6721 ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg
     6781 ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac ctcacgcagc
     6841 gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac cacccggcca
     6901 gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa atcagccagc
     6961 gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc ggtggtcata
     7021 cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact ctctttgagc
     7081 atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg
     7141 tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg ggtcaggccc
     7201 ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt cggctgggcg
     7261 gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg
     7321 atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata gtcctgcata
     7381 tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt attctgcgga
     7441 atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat cgtgttctct
     7501 ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat cgactttccc
     7561 gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt taaattcggc
     7621 aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct cttttaccac
     7681 cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca gtgcggcata
     7741 gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga tcttctcgcc
     7801 gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct ctggatcaat
     7861 agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg cattgtgctg
     7921 cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc gtccaacttc
     7981 cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt ccggacacat
     8041 tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg tgtagttcac
     8101 cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag cgggaatacg
     8161 cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca gcgaggcaag
     8221 ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg ccggcggcac
     8281 cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg tggcgctggg
     8341 atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc ctggcgaact
     8401 catagcgtag ttatgagccg ccgggatcgc gcacaggtag cgcgtctgtt gtgtgaactg
     8461 acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc gcgggatcag
     8521 tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat cagcaggtga
     8581 tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt tatgctcacc
     8641 gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa atgtgttgag
     8701 gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg ccggttcaat
     8761 ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca gcaccggcgc
     8821 gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt tacccagttg
     8881 taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg cgaggttatc
     8941 cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa ccgcgatgct
     9001 gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc agtagccttt
     9061 ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc tcatcctcgc
     9121 tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca
     9181 ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac gcacctgatt
     9241 ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt cgctgtcaca
     9301 ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag caataacctg
     9361 ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca gacaggccag
     9421 agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga gatgccacag
     9481 cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac tgcgttcgcg
     9541 cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt tttctgacgc
     9601 cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac gcgcggaacc
     9661 ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt ccgggctggc
     9721 ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa ccgggttttg
     9781 cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg tgatatcaat
     9841 caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg cggcgcgggc
     9901 acccatctgt tcaaacgtgg tttgttcagg aattgcctgg cgttccacca cgttcagcga
     9961 ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga acgactgcta
    10021 ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg ccccgaccat
    10081 caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga gtttttgctg
    10141 gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg cgaaacatcc
    10201 tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg caagtgttgc
    10261 catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca caaaatgata
    10321 aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag caaacgcgca
    10381 ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg ttgtggttac
    10441 aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg tcctgcgccg
    10501 ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt cattacgccc
    10561 ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta ccgcaacgcc
    10621 cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc cgactggccg
    10681 cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca gcgtattgtt
    10741 tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt gatcgccagc
    10801 ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg ccagtggagc
    10861 aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag cgccgaagcc
    10921 gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga ttcggcgctg
    10981 gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta cgacgacaaa
    11041 agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa acaagcggca
    11101 gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat caaattaccg
    11161 ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa tctctggacg
    11221 ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc gaaattgccc
    11281 gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca gcttggtggg
    11341 gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg tgatcagaaa
    11401 gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt acaaaacaag
    11461 caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc catgcaagtg
    11521 ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca cctgcggcgg
    11581 cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc cgataatcaa
    11641 caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc ccaaaccacc
    11701 cagcagcgcc gcgtctatgg catcgcccgt cacgttctgc gccgtccaca aaccgttaat
    11761 ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca gcaacgaact
    11821 aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc acatcggcat
    11881 caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca tcaataaccc
    11941 tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg cgccgagcgg
    12001 gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt cagccagcgc
    12061 cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca gcgggctggc
    12121 aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg gctgcggtgg
    12181 cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc ccgccgccgc
    12241 cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa tcatgggcga
    12301 aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca ttaagttttc
    12361 acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg atgcgaaaaa
    12421 accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca gcagtgcatt
    12481 aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa tcactttttt
    12541 gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca tcgcgccgcc
    12601 ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct ggatctgcac
    12661 cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag cgaggaatac
    12721 tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg attgtttatt
    12781 cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc acatgttaac
    12841 atatccaaat aagatcgata acgataatta atttcattat catggaagtt cgtatgtctg
    12901 gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta ctgattatcg
    12961 cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta gtgctggagg
    13021 ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg cggctgccgc
    13081 gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg ttaatgcaaa
    13141 ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc ggagccagct
    13201 ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa caactggcga
    13261 tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc agtcagggtg
    13321 gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg gcggtgctgg
    13381 aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa ctgcgtttct
    13441 ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg ctgatcccgg
    13501 ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt ttgagcctcg
    13561 gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg attggtctgc
    13621 tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt gcctttatcg
    13681 gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc tggtcgctgc
    13741 ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc gggcgggtga
    13801 ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca ccggtgctga
    13861 tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc gccgattact
    13921 catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg gattacgcag
    13981 cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg atgcgccgcg
    14041 cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg cgctgttgat
    14101 tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta acccgctcgg
    14161 cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg tggcgatggt
    14221 gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg gcattatcac
    14281 ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc ggttgattat
    14341 tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt tgctgaaagc
    14401 gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca acggcctgac
    14461 gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg ccgccacctt
    14521 gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg cgctgggcgt
    14581 cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta ccgctgcggc
    14641 aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca ttgcccgacg
    14701 cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag cgctgttact
    14761 gctggcggcc gatctctgcg cccaacaact gtttatgccg tatcaacttc cggttggcgt
    14821 cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg agtctcgcaa
    14881 aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg atatggcaaa
    14941 tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac ggcaattatc
    15001 ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct gatgacgcct
    15061 gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag taaagaagtt
    15121 gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat caccgtgcag
    15181 gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg gcgcaaagag
    15241 gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct ggcagatcaa
    15301 agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat ggtgctggcc
    15361 caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat cagtcatcag
    15421 attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac cctggcggcg
    15481 gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc attgcgggaa
    15541 gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact gattgagcgc
    15601 atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc gcttgtggtg
    15661 ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc tgccatcatg
    15721 gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca agccaccatc
    15781 ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc ttttttcact
    15841 ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt gacatttgct
    15901 tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag ttccgcaacg
    15961 tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc gagggaaata
    16021 gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt tttaattcct
    16081 gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc aagttgattt
    16141 ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt gatctccagt
    16201 ttattacgga cgttttctat cgactctttc accaccaacg cagagatata atcgatatac
    16261 ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag cgtccaggat
    16321 gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc tttcattttt
    16381 tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc ctcttttaat
    16441 tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac agactgaaac
    16501 ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag atcaagaacg
    16561 cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc gggagtaata
    16621 acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc tgcgcaggca
    16681 aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc gattagatta
    16741 agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa atgagcgtca
    16801 cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag tccaataggg
    16861 gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc tgaagtttgt
    16921 aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca tcaaacctat
    16981 aaatcaaaca aataaaaaac ccataattat tttctgta
//
LOCUS       DAJTOW010000081        16940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTOW010000081 DAJTOW010000000
VERSION     DAJTOW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.081"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..185
                     /locus_tag="OO244_002867"
     CDS             <1..185
                     /locus_tag="OO244_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5664619.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="OO244_002868"
     CDS             complement(210..401)
                     /locus_tag="OO244_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5664620.1"
                     /translation="MFALVLFVCYLDDGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="OO244_002869"
     CDS             complement(506..742)
                     /locus_tag="OO244_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5664621.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO244_002870"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO244_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO244_002871"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO244_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO244_002872"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO244_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5664622.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHILAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="OO244_002873"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="OO244_002873"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5664623.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="OO244_002874"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="OO244_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5664624.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="OO244_002875"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="OO244_002875"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5664625.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="OO244_002876"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="OO244_002876"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5664626.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="OO244_002877"
     CDS             complement(10860..12233)
                     /locus_tag="OO244_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664627.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALIGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="OO244_002878"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="OO244_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5664628.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="OO244_002879"
     CDS             complement(13344..13583)
                     /locus_tag="OO244_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664629.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="OO244_002880"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="OO244_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5664630.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="OO244_002881"
     CDS             13960..14247
                     /locus_tag="OO244_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5664631.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14917..15060
                     /locus_tag="OO244_002882"
     CDS             14917..15060
                     /locus_tag="OO244_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5664632.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15073..15282
                     /gene="cspE"
                     /locus_tag="OO244_002883"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15073..15282
                     /gene="cspE"
                     /locus_tag="OO244_002883"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5664633.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15448..16257
                     /gene="rlmA"
                     /locus_tag="OO244_002884"
     CDS             15448..16257
                     /gene="rlmA"
                     /locus_tag="OO244_002884"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5664634.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="OO244_002885"
     CDS             complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="OO244_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5664635.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4454 a         4108 c         4299 g         4079 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgtcatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgccaggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcat ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccaatcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 attatttatt agcgaaacgt ttctctcttg atttttttgc tcactcatca atttttctta
    14521 ttttaaattt acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
    14581 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc agaaaatgtg
    14641 atattgcacg cactatgtaa taacttctcc ctctggcctg gaacaactga acttattgaa
    14701 ctatgttaga aactacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
    14761 atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
    14821 ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat
    14881 aaaagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
    14941 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
    15001 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
    15061 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
    15121 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
    15181 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
    15241 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
    15301 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
    15361 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
    15421 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
    15481 ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac atcagtttga tatggcgaaa
    15541 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
    15601 gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
    15661 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
    15721 attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc
    15781 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
    15841 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
    15901 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
    15961 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
    16021 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
    16081 cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
    16141 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
    16201 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
    16261 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
    16321 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
    16381 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgaaggaaa
    16441 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
    16501 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
    16561 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
    16621 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
    16681 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
    16741 ggggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
    16801 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
    16861 cccccagcaa acatgcatac atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg
    16921 catagcgatt gatgtgcata
//
LOCUS       DAJTOW010000082        16807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTOW010000082 DAJTOW010000000
VERSION     DAJTOW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.082"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..1027
                     /gene="mdtA"
                     /locus_tag="OO244_002886"
     CDS             <1..1027
                     /gene="mdtA"
                     /locus_tag="OO244_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5664636.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
     gene            1027..4149
                     /gene="mdtB"
                     /locus_tag="OO244_002887"
                     /pseudo
     CDS             1027..4149
                     /gene="mdtB"
                     /locus_tag="OO244_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            4150..7215
                     /gene="mdtC"
                     /locus_tag="OO244_002888"
     CDS             4150..7215
                     /gene="mdtC"
                     /locus_tag="OO244_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5664637.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMLVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7216..8631
                     /locus_tag="OO244_002889"
     CDS             7216..8631
                     /locus_tag="OO244_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664638.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8628..10031
                     /gene="baeS"
                     /locus_tag="OO244_002890"
     CDS             8628..10031
                     /gene="baeS"
                     /locus_tag="OO244_002890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5664639.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10028..10750
                     /gene="baeR"
                     /locus_tag="OO244_002891"
     CDS             10028..10750
                     /gene="baeR"
                     /locus_tag="OO244_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5664640.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10941..11273
                     /locus_tag="OO244_002892"
     CDS             10941..11273
                     /locus_tag="OO244_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5664641.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11420..12781
                     /gene="yegQ"
                     /locus_tag="OO244_002893"
     CDS             11420..12781
                     /gene="yegQ"
                     /locus_tag="OO244_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5664642.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKF
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13123..13440)
                     /locus_tag="OO244_002894"
     CDS             complement(13123..13440)
                     /locus_tag="OO244_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664643.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13846..14745
                     /gene="yegS"
                     /locus_tag="OO244_002895"
     CDS             13846..14745
                     /gene="yegS"
                     /locus_tag="OO244_002895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5664644.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14827..15600)
                     /locus_tag="OO244_002896"
     CDS             complement(14827..15600)
                     /locus_tag="OO244_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5664645.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15706..16746)
                     /gene="gatD"
                     /locus_tag="OO244_002897"
     CDS             complement(15706..16746)
                     /gene="gatD"
                     /locus_tag="OO244_002897"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5664646.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         3877 a         4488 c         4414 g         4028 t
ORIGIN      
        1 ttacctcacc gggcttggca ccattaccgc cgctaatact gttacggtgc gcagccgcgt
       61 ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag caggcgattt
      121 actggcagaa attgacccca gccagttcaa agttgcatta gcacaaaccc agggccaact
      181 ggcaaaagat aaagccacgc ttgctaacgc ccgccgtgat ctggcgcgtt atcagcaact
      241 ggcaaaaacc aatctcgtct cccgtcagga gctggatgcc caacaggcgc tggtcagtga
      301 aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga
      361 ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt ctcaagcaag ttgatgttgg
      421 taaccaaatc tccagtggtg ataccaccgg aattgtggtg atcacccaga cgcatcctat
      481 cgatttagtc tttaccctgc cggaaagcga tatcgctact gtagtgcagg cgcaaaaagc
      541 cggaaaaccg ctgatggtag aagcctggga tcgcaccaat tcgaagaaat taagtgaagg
      601 cacgctgtta agcctcgata accaaatcga tgccacaacc ggtacgatta aagtgaaagc
      661 acgctttaat aatcaggatg atgcgctgtt tcccaatcag tttgttaacg cgcgcatgtt
      721 agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc gccctgcaaa tgggcaatga
      781 aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc tggtgacgcc
      841 gggcattcag gacagtcaga aagtggtgat ccgtgcaggt atttctgcgg gcgatcgcgt
      901 ggtgacagac ggcattgatc gcctgaccga aggggcgaaa gtggaagtgg tggaagccca
      961 gagcgccacc actccggaag agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg
     1021 ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct gtttattatg
     1081 cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac tcgccgggat tatcggttat
     1141 cgcgccctgc ccgtttcggc gctgccggaa gtggactatc cgaccattca ggtggtcacg
     1201 ctctacccag gtgccagccc ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc
     1261 cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca
     1321 gttatcactt tgcagttcca gctaacatta ccgctcaatg tcgccgagca ggaagtgcag
     1381 gccgcgatta acgctgcgac caacttgttg ccgagcgatc tgcctaaccc gccggtttac
     1441 agcaaagtga acccggcaga tccgccgatc atgacgctcg ccgtcacctc aaccgccatg
     1501 ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt
     1561 tccggcgtcg gcctggtgac gctttccggc ggtcagcgtc cggctgttcg cgtcaaactt
     1621 aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtgcgcac cgccattacc
     1681 ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc cttcccgtgc ggtcacgctt
     1741 tccgcgaacg accagatgca atccgccgaa gagtatcgcc agctaatcat cgcctaccag
     1801 aacggcgcgc caattcgtct gggcgatgtc gcaactgtag agcaaggtgc agaaaacagc
     1861 tggctcggcg cgtgggcgaa caaagaacag gccattgtga tgaatgttca gcgccagccc
     1921 ggtgctaaca ttatctccac cgccgacagc attcggcaga tgctgccaca gctcactgag
     1981 agtctgccga aatcggtgaa ggtgacagtg ctttccgatc gcaccaccaa tatccgcgca
     2041 tccgtcgatg atactcagtt tgaattgatg atggctatcg cgctggtagt catgattatc
     2101 tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgctgt accgctgtcg
     2161 ttaatcggca ctttcgcggt tatggtgttt ctcgattttt caatcaataa cctgacactg
     2221 atggctttaa ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac
     2281 atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa gggcgcaggt
     2341 gaaatcggct ttaccattat ctcgctgacc ttctcactga ttgcggtgtt gatcccactg
     2401 ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac cctggcggta
     2461 gcgattttga tctcagcggt ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg
     2521 ctcagccagg agtcgttgcg taaacagaac cgcttctccc gtgcctcgga aaaaatgttc
     2581 gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg
     2641 accttaagcg tggcactcag cacgctgctg cttagcgtgc tgctgtgggt gttcattccg
     2701 aaaggtttct tcccggtaca ggacaacggc attattcagg gcactttgca ggcaccgcaa
     2761 tccagctcct ttgccaatat ggcccagcga caacgccagg tcgcggacgt gattttgcag
     2821 gatccggcag tgcaaagcct gacctcattt gttggcgttg atggcactaa cccgtcgctg
     2881 aatagtgcac gtttacaaat caacctcaaa ccgttggatg aacgtgatga tcgggtgcaa
     2941 aaagtcatcg cccgtctgca aacggcggta gataaagtgc cgggcgtcga tctcttcctg
     3001 caaccaacgc aggacctgac catcgatact caggtcagcc gcacccagta ccagtttacc
     3061 ttgcaggcca cgtcactgga tgcgctcagt acctgggtgc cacagttgat ggaaaaactc
     3121 cagcaactgc cacagctttc tgatgtctcc agcgactggc aggacaaagg gctggtggcg
     3181 tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga tcagcatggc ggacgtcgat
     3241 aacgccctgt acaacgcgtt tggtcagcga ctgatttcca ctatttatac tcaggctaac
     3301 cagtatcgcg tggtgctgga gcacaatacc gaaaataccc caggcctcgc ggcgctggat
     3361 accattcgcc tgaccagcag cgacggtggc gtggtgccgt taagctcaat tgccaaaatt
     3421 gagcagcgtt ttgcgccgct ttccatcaac catctggatc agttcccggt aacaaccatc
     3481 tcctttaacg tgccggataa ctattcgctg ggcgatgcgg tgcaggcgat tatggacacc
     3541 gaaaagacgc tgaatctgcc ggtggatatc accacgcaat tctagggcag caccctcgcc
     3601 ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat
     3661 atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct ctcgacgcta
     3721 cccaccgcag gggtcggcgc gctgctggca ttaatgattg ccggtagcga actggatgtg
     3781 attgcgatta tcggcattat tttgctgatc ggtattgtga agaagaacgc catcatgatg
     3841 atcgacttcg cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac
     3901 caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ccctggcggc tctgcttggc
     3961 gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc
     4021 ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc tgttcaccac gccggtgatt
     4081 tatctgctgt tcgaccgcct ggcattgtgg accaaaagtc gctttgctcg ccatgaagag
     4141 gaggcgtaag tgaagttttt tgccctcttc atttaccgcc cggtggcgac gattttactg
     4201 tcggtcgcca ttaccctgtg cggcatactg ggtttccgta tgctgccggt cgccccgctg
     4261 ccgcaggtcg attttccggt gattatggtc agcgcctcgc tgcccggtgc gtcgccggag
     4321 acaatggcgt cttccgttgc cacgccgctg gagcgctcac ttgggcgcat tgccggggtt
     4381 agtgaaatga cctccagcag ttcgctcggc agcacgcgca ttattttgca gtttgatttt
     4441 gaccgggata tcaacggcgc agcgcgtgat gtgcaggcgg cgatcaacgc tgcacaaagt
     4501 ttgctgccca gcgggatgcc cagccgcccg acctatcgca aagcgaaccc gtcggatgcg
     4561 ccaattatga tcctcacgct gacatccgat acttattcgc agggtgaact gtacgatttc
     4621 gcctcgacgc agctggctcc gacgatttcg caaatcgacg gtgtcggtga tgtcgatgtc
     4681 ggtggcagct cgctgcccgc cgtgcgcgtc gggctgaccc cgcaggcgct gtttaatcag
     4741 ggcgtgtcgc tggacgacgt acgcactgcc atcagcaatg ccaacgtgcg taaaccgcag
     4801 ggggcgctgg aagatggcac tcaccgctgg cagatccaga ccaacgatga actgaaaacc
     4861 gccgctgaat atcagccgct gattattcac tacaacaacg gcggcgcggt tcgtctgggc
     4921 gatgtggcga cggtgactga ctcagtgcag gatgtgcgca acgccgggat gaccaacgcc
     4981 aaaccggcta ttttactgat gatccgcaaa ctgccggaag ccaatattat ccagacggta
     5041 gacagcatcc gggcaaaatt accagagttg caggagacca ttccggcggc gattgatctg
     5101 caaattgccc aggatcgctc ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg
     5161 ctgattatct cggtggcgct ggtgattctg gtggtctttt tattcctgcg atcgggtcgc
     5221 gccactatta ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg
     5281 tacctgtgcg gattcagtct caataatctt tcgctgatgg cgctcaccat tgctactggt
     5341 ttcgtggtgg atgacgccat tgtggtgctg gaaaacattg cccgtcatct ggaagcgggg
     5401 atgaaaccac tacaggccgc gctgcaaggt actcgcgaag tcggctttac ggtgctgtcg
     5461 atgagtctgt cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg attgccgggc
     5521 cgactgttac gcgagtttgc cgtgacgctt tctgtcgcca ttggtatttc gttgctggtt
     5581 tccctgacgc taacgccaat gatgtgcggc tggatgctaa aagccagcaa gccgcgtgag
     5641 caaaagcgac tgcgtggttt tggccgcatg ttggtagccc tgcaacaagg ctacggcaag
     5701 tcgctgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct tggcaccatt
     5761 gcgctgaata tctcgatccc gaaaaccttc ttcccggagc aggacacggg cgtactgatg
     5821 ggtgggatac aggcggatca gagtatttcg tttcaggcga tgcgcggcaa attgcaggat
     5881 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt taccggcggt
     5941 tcacgagtta acagtgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
     6001 acggcgcagc aaattatcga ccgtctgcgc gtgaagctgg cgaaagaacc gggggcgaat
     6061 ctgttcctga tggcggtaca agatattcgc gttggtgggc gtcagtcgaa cgccagctac
     6121 cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc taaaatccgc
     6181 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
     6241 gcggagatga atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtacag
     6301 gccgccaaca gtctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
     6361 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt
     6421 gcgctggaaa aaatgttagt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc
     6481 gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc
     6541 tcgaccatct cgttcaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
     6601 gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc gcggcagttt tgccggcacg
     6661 gcgcaggttt tccaggagac gatgaactcg caggtgatcc tgattatcgc cgccatcgcc
     6721 acggtgtata tcgtgctggg tatcctttac gagagttacg tacatccgct gacgattctc
     6781 tccaccctgc cctcggcggg cgttggagcg ctgttggcgc tggagctgtt caatgccccg
     6841 ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc
     6901 attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa
     6961 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgaccac cctggcggcg
     7021 ctgtttggcg cgctgccgct ggtattgtcg ggcggtgacg gctcggagct gcggcaaccc
     7081 ctggagatca ccattgtcgg cggtctggtg atgagccagc tcctgacgct gtataccacg
     7141 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
     7201 acggtaaccg agtaaatgac agatcttccc gacagcaccc gctggcaatt atggattgtg
     7261 gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca
     7321 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
     7381 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
     7441 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
     7501 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg tgcgatgatg
     7561 gtgccggtcg gcaggttgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
     7621 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
     7681 ctgctggtag aatacgcgtc gtggcactgg atctttttga tcaacattcc ggtggggatt
     7741 atcggtgcga tcgccacatt gatgttaatg ccgaactaca ccatgcagac gcggcgcttt
     7801 gatctttccg gctttttatt gctggcggtt ggcatggcgg tattaaccct ggcgctggac
     7861 ggcagtaaag gtacaggttt atcgccgctg gcgattgctg gcctggtcgc agttggcgtg
     7921 gtggcactgg tgctttatct gctgcacgcc agaaataaca accgcgccct gttcagtctg
     7981 aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
     8041 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg
     8101 ccgtttcatg ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga
     8161 attgtggtac aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg
     8221 ggtctgtcgc tggtcaccct gttgtttatg actaccgctc tgttgggctg gtactacgtt
     8281 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cccgtttctc ctccatgaac
     8341 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggaaacag cctgctgtcg
     8401 atgattatgc aattgtcgat gagtatcggc gtcaccatcg ccgggctgtt gctggggctt
     8461 tttggttcgc agcatatcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
     8521 tggcttagca tggcgtttat catcgccctt ccggcgttca tctttgccag agtgccgaac
     8581 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
     8641 gacccggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga
     8701 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaaac
     8761 acggcaatga acagcgatta cagctgttaa gtgatgcgct tggcgagcag tatgcgcagc
     8821 atggtaactg gcgcttcctg cgcaacaacg atcgctttgt ctttcagatc ctgcgttcat
     8881 ttgaacacga taattcggaa gataaacccg gcccgggtat gccgccgcac ggctggcgta
     8941 cccagttctg ggtggtcgat caaaacaaca aagtactggt tggtccgcga gcgccgattc
     9001 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagtgggc gcggtgattg
     9061 cctcccctgt tgagcggctg acccgcaata ctgatatcaa tttcgataaa caacagcggc
     9121 aaaccagctg gctgatcgtc gccctggcaa cgttactcgc ggcgctcgcc acctttctgc
     9181 tggcgcgcgg tttgctggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg
     9241 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc
     9301 aagacttcaa ccagctcgcc agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta
     9361 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
     9421 ccattcagga tggcgtgcgc aaattcacgc cggagacggt ggcgtcttta caggcggagg
     9481 tcggtacact gaccaaactg gttgacgatc ttcatcagtt gtcgatgtct gatgaaggcg
     9541 ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg
     9601 catttcgcga acgattcgcc agccgtggcc tgaaactgca attttccctg ccagacagta
     9661 tcaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta ctggaaaaca
     9721 gcctgcgcta cactgacagc ggcggcagcc tgaaaatctc tgccgagcag cacgacaaaa
     9781 cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat
     9841 tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg cgccagcggc ggttccgggc
     9901 tggggctggc gatttgcctg aacattgttg aagcccataa tggccgcatt attgccgccc
     9961 attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga
    10021 gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga
    10081 tgaaccgaag ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac
    10141 gcttatcagc cacggcgatc aggtactggc gtatgtgcgc cagacaccac cggatctgat
    10201 cctgttggat ctgatgctcc ctggcaccga tggcctgaca ttatgccggg aaattcgtcg
    10261 tttttccgat gttccgatcg tgatggtgac ggcaaaaatc gaagagatcg accgcctgct
    10321 ggggctggag attggcgccg atgactacat ctgcaagccg tacagcccac gggaagtggt
    10381 agcgcgtgtc aaaaccattt tgcgccgttg taaaccgcaa cgcgaacttc agcaacagga
    10441 tgccgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat
    10501 gcttgacctg acgcctgcgg aatttcgtct gcttaaaacg ctctctcacg aaccaggaaa
    10561 agtgttctcc cgcgagcaat tgctcaatca tctttatgac gactaccgcg tagtaaccga
    10621 ccgcaccatt gacagccaca ttaaaaatct gcgccgcaag ctggaatctc tcgacgccga
    10681 acagtcattt atccgcgccg tttacggcgt tggttaccgc tgggaagccg acgcctgccg
    10741 catcgtttag ttttagcgac attactttgt tagccggaga tggcgttcag acccgatccg
    10801 gcaacattat tccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc
    10861 agaaaatcgc tcatacttta atcagtaaac agcaccttta gatgctgttt tcgataagca
    10921 atttcaatca aggagtcatt atggctggtt ggtttgaact cagtaagagc agtgataatc
    10981 agttccggtt tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata
    11041 cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag
    11101 agcgctatga gaaaaaaacg gcaagcaacg gcaaattcta tttcaatctg aaagccgcta
    11161 atcatcaaat tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa
    11221 ttgcctccgt taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat
    11281 gccgggcgtg ggttgttggc gtccggcgct ttaccagatc ccccctacag cgctacaatg
    11341 cccgccctta aagtgggggc ctcccctaac cgcttcatca ggtgaagcgg atctgacctg
    11401 tcatcagaac gagagaatta tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa
    11461 aaatatgcgt tacgctttcg cttatggcgc agatgctgtt tatgcgggcc agccgcgtta
    11521 ctccctacgt gtgcgcaaca acgaattcaa ccacgaaaat cttcagctcg gcatcaatga
    11581 agcccacgcg ctggggaaaa agttttatgt cgtggtcaac attgcaccgc acaacgccaa
    11641 gctgaaaacc tttatccgtg acctgaaacc ggtggtggaa atggggccgg atgcgctgat
    11701 tatgtccgat ccagggctga ttatgctggt gcgtgagcac ttccctgaaa tgccgatcca
    11761 cctctcggtg caggctaacg ccgtgaactg ggcgacggtg aaattctggc agcaaatggg
    11821 actgacccgc gtgatcctct ctcgcgagct gtcgctggaa gagattgaag agatccataa
    11881 tcaggtgccg gatatggaga tcgagatctt cgttcacggc gcactgtgca tggcctactc
    11941 cggtcgctgc ctgctctctg gctatatcaa caagcgcgac ccgaaccagg gcacctgcac
    12001 caacgcctgc cgctgggagt acaacgtcca ggaagggaaa gaagatgacg ttggcaacat
    12061 cgtacacaag ttcgagccga ttccggtgca aaatgttgag ccgacgctgg gtatcggcgc
    12121 gccaaccgac aaagtgttta tgatcgaaga agcccagcgt ccgggcgagt atatgaccgc
    12181 gtttgaagat gagcacggca cttacatcat gaactcgaaa gatctgcgcg ccatcgccca
    12241 tgtagaacgc ctgaccaaaa tgggcgtgca ttcgctgaaa atcgaaggtc gtaccaaatc
    12301 tttctactat tgcgcacgca ccgcacaggt ttaccgcaaa gctatcgatg acgccgctgc
    12361 gggaaaaccg ttcgatacca gcctgctgga aactctggaa ggtctggcgc atcgtggcta
    12421 taccgaaggt ttcctgcgtc gtcatactca cgacgattat cagaactacg aatacggtta
    12481 ttcagtttct gaccgccagc agtttgttgg tgagtttacc ggtgagcgca agggggacct
    12541 cgcagcggta gcggtgaaaa ataaattctc cgttggcgac agtcttgagc tgatgacgcc
    12601 gcaaggcaac attaacttta cccttgagca catggaaaac gccaaaggcg aagctatgcc
    12661 ggtcgcacca ggcgatggtt atactgtgtg gctcccggtc ccgcaggatc ttgagctaaa
    12721 ttacgcgctg ctaatgcgta atttctccgg ggaaaccacg cgtaaccccc acggtaagtg
    12781 attaatttcg attatttttc ccggatggaa aattcttaga aaccgatcac atacagctgc
    12841 atttattaag gttatcatcc gtttcgctga aaaacataat ccataaaatg ctagctgtac
    12901 caggaaccac ctccttaacc tgtgtaatct cccttacacg ggcttatttt ttgtacataa
    12961 gctgtaaaga aggattgatc cctgataacc gtcttccatt gaccacatcg atagaatccc
    13021 ccttcatagc acaatgcctt tcacgtaacg gtatcgtgct cgcccaggtt ccggctacgc
    13081 acagccagaa cgcgcatgtt tgacgcttac aaaaaaatat tctcactctc cacatttgaa
    13141 tgtcagacga gcgacgccat gtaatcctgc accttctgtc ttcaggtcaa ctatctgcat
    13201 ttttttgccc tgagtaacac agaaatgggc tgcatcattt tttactatat tttctgcacc
    13261 agatattctg cccctggcta aagaagcttc ggcttcggtg tagtattggt tatcgagttt
    13321 acgctgaata ttacttttat atgcaagacc aaatttaccg atacttgttt catcattatg
    13381 cacagcacaa ccagatataa gaaaaatact aattaatgat atagcagcta tctttttcat
    13441 ctcaccttcc cccattaaat accaacgaca ctctctagtg tttaaatata ataatggcat
    13501 gattattata attgaatagg attataataa atgttctgta caacatttcc tacataagta
    13561 ggaattacgg acattgaggc ccttcagggt aactccatgg gggctttaat atattatatt
    13621 gaagatgcca ctgtttagtt gaatattagg tatatgctct tttttgaaat ttatcggtgg
    13681 cagccgttag tattcgctgt ccccattgca agctcctggt ggtaaccact gaatcctcca
    13741 tacttgaact gactttttat cctccgactt tcatcctgtt ctgactccac cttttgtttt
    13801 ctgctctaca ctatctacag accaatcata aaggcacata cgatcatggc agaatttccc
    13861 gccagcttac tgattcttaa tggtaaaagt actgacaatc tacccttgcg cgaagcaatt
    13921 atgctgttgc gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggtgat
    13981 gccgcacgat ttgtagagga ggcccggaag ttgggcgtcg caacggtgat tgccggtggt
    14041 ggcgatggca ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatgacata
    14101 cccgcgctgg gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt
    14161 cctgaggcac tggataaggc gctgaaactg gcaattgccg gtaatgccat tgcgatagat
    14221 atggcgcagg tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg
    14281 cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc
    14341 attcatggct taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa
    14401 aactttcact ggcaaggtga cgccctggtc attggtattg gtaacgggcg tcaggccggt
    14461 ggcggtcaac aattgtgccc gaacgcgtta attaacgatg gcttgctgca actgcgcatt
    14521 tttaccggcg atgaaattct tccggctctc gtatcaacct taaaatctga cgaagataac
    14581 ccgaatatta tcgaaggcgc ttcgtcgtgg tttgatatac aagccccaca cgaaatcact
    14641 tttaatcttg atggcgaacc gttgagtggg caaaacttcc atattgaaat acttccggcg
    14701 gcgttgcgtt gtcgattacc accagattgt ccattattgc gttaatcaaa ctattctctc
    14761 ttgaaccagg gagaatccgt aatgccggat gcggtgtaaa caccttatcc ggcatacaga
    14821 acaatactac accgtaatta attttacccg tgtcttctgc aatgccaatt tatccacctc
    14881 tgacacagca tcatcggtaa caataatgtc aattttttcg attggcagta cctgattaaa
    14941 accgcgacga ttaaatttcg atgaatcgag aacggcaatc actttatttg cggcggtgac
    15001 catcgccccg ctaatcgaat aaccttcatt aaaggtcgta ataccattaa ccgcatcaat
    15061 accatcagca ccgacaaaca ttaaatcggc attaatatcc tgcaatgaac gctcggcaat
    15121 ggaaccatgc atcgagcgcg ttttatggcg gaccgtgcca ccacagacga ccagagtaat
    15181 gtctttattt tcggaaaggg caaacgctgc cgggagactg ttggtaatca ccgtgatatt
    15241 tttagcggtc attaatcctt cggcgataag cattgtggta cttccgctgt cgagaataac
    15301 ggtcatccct tcatggatca ttttgactgc cgcctgcgct attctgtttt ttggcgcgct
    15361 ggcgagctga aatcgctctt taaacccgac ttcctgggtc tcggtttcac tattaccgga
    15421 cattattttc gccgcaccgc catgaaagcg cgtaaccacg cctttttgtt cgagaaagcg
    15481 caaatcggca cggattgtcg cttccgaggc agcaaatact cccgccagat cctgaacaag
    15541 cacggttccc tgttcattca ctaattggat gatcttattc cttcgctcga atgaattcat
    15601 cgtcatttac ccatattcaa ttgtggctag tgtaaacgaa gggggaccat tggtgaatga
    15661 tctgttttgc acaataccgg gccagcatca cgctggcccg cggtttcatg gaatgagcaa
    15721 cactttgccc tgcatggcat tacgagcgat gtcacgcacc gcctgggtga agctttcaaa
    15781 gcttccacgg tgagcgatta atggctccag gcttaactta cgttctgtca gcaaccggct
    15841 cgccgtttcc cactcctgcc ccggccaagg gctggaatag ttcatccaac tgccgataac
    15901 cgtcagctct ttacgcaata ttttgccaaa cgttgccgat gttaaatgca gatcctgatg
    15961 caacgtgccc accagcgcca gttgggcatg aggaccggca atctctaccg ccagttcgac
    16021 agtttgcggt acgccagccg tctcgaggat aagctgatta aagcgcagtt cgcgtaaaac
    16081 gctctgcatt tgcggcgcgc tcatttcgct actgttaaat gtttgcatcg caccgaaaga
    16141 ttttgccagt gccagttttt ctgagctaat gtcgatcgcc gtcacactct ttgctcccag
    16201 cgcgacagcg cactgaatag ccagcaggcc aatggttccg gcaccaataa taataacgtt
    16261 tttattctca caaccttgcg ctaaatgaaa agcatgcagg ccaacggtaa tcggctcaat
    16321 aaaagcccca tcctcaatag gcatatccgt gggtagagca aagacatttt ttcgcttaac
    16381 gacaatatat tcagcaaatc caccatcacg ccgcgagcca ataaaatcat attttgcgca
    16441 ctgggaataa aaccctttca aacactctgg acaagtaaaa cagggtaata acggcacaca
    16501 ggcaaccgca tcgccagggt gtaaatcatc aacaccggat cccaccgcat caatatagcc
    16561 gctaaattca tggcctaacg ttattggata ataatgtgca ccatttttaa atatcctggg
    16621 taaatcggaa ccacataagc ccgagctggc aatttttacc cgcacctcat cctgatgttt
    16681 aatttcagga atgacgcttt ttgcaacgcg cacgatacca tcagtatcat tcactactga
    16741 tttcataaaa actcctgatt gttggtaatg actccaactt actgatagtg ttttatgttc
    16801 agataat
//
LOCUS       DAJTOW010000083        16427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTOW010000083 DAJTOW010000000
VERSION     DAJTOW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.083"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..574
                     /gene="ybhA"
                     /locus_tag="OO244_002898"
     CDS             <1..574
                     /gene="ybhA"
                     /locus_tag="OO244_002898"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5664647.1"
                     /translation="DPMPVNKALQLIEMLNEHHIHGLMYVDDAMVYEHPTGHVIRTSN
                     WAQTLPPEQRPTFTQVASLAETTQQVNAVWKFALTHDDLPQLQHFGKHVEHELGLECE
                     WSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENVVAFGDNFNDISMLEAAGTGVAMGN
                     ADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(575..1633)
                     /gene="modC"
                     /locus_tag="OO244_002899"
     CDS             complement(575..1633)
                     /gene="modC"
                     /locus_tag="OO244_002899"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5664648.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1636..2325)
                     /gene="modB"
                     /locus_tag="OO244_002900"
     CDS             complement(1636..2325)
                     /gene="modB"
                     /locus_tag="OO244_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5664649.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2325..3098)
                     /gene="modA"
                     /locus_tag="OO244_002901"
     CDS             complement(2325..3098)
                     /gene="modA"
                     /locus_tag="OO244_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5664650.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3265..3414)
                     /gene="acrZ"
                     /locus_tag="OO244_002902"
     CDS             complement(3265..3414)
                     /gene="acrZ"
                     /locus_tag="OO244_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5664651.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3543..4331
                     /gene="modE"
                     /locus_tag="OO244_002903"
     CDS             3543..4331
                     /gene="modE"
                     /locus_tag="OO244_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5664652.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4399..5871
                     /gene="modF"
                     /locus_tag="OO244_002904"
     CDS             4399..5871
                     /gene="modF"
                     /locus_tag="OO244_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5664653.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6132..7148
                     /gene="galE"
                     /locus_tag="OO244_002905"
     CDS             6132..7148
                     /gene="galE"
                     /locus_tag="OO244_002905"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5664654.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7158..8204
                     /gene="galT"
                     /locus_tag="OO244_002906"
     CDS             7158..8204
                     /gene="galT"
                     /locus_tag="OO244_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5664655.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8208..9356
                     /gene="galK"
                     /locus_tag="OO244_002907"
     CDS             8208..9356
                     /gene="galK"
                     /locus_tag="OO244_002907"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5664656.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9350..10390
                     /gene="galM"
                     /locus_tag="OO244_002908"
     CDS             9350..10390
                     /gene="galM"
                     /locus_tag="OO244_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5664657.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10593..11345
                     /gene="gpmA"
                     /locus_tag="OO244_002909"
     CDS             10593..11345
                     /gene="gpmA"
                     /locus_tag="OO244_002909"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5664658.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11512..12564)
                     /gene="aroG"
                     /locus_tag="OO244_002910"
     CDS             complement(11512..12564)
                     /gene="aroG"
                     /locus_tag="OO244_002910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5664659.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            12880..13260
                     /locus_tag="OO244_002911"
     CDS             12880..13260
                     /locus_tag="OO244_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5664660.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13374..14315
                     /gene="zitB"
                     /locus_tag="OO244_002912"
     CDS             13374..14315
                     /gene="zitB"
                     /locus_tag="OO244_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5664661.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(14312..15031)
                     /gene="pnuC"
                     /locus_tag="OO244_002913"
     CDS             complement(14312..15031)
                     /gene="pnuC"
                     /locus_tag="OO244_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5664662.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15069..16112)
                     /gene="nadA"
                     /locus_tag="OO244_002914"
     CDS             complement(15069..16112)
                     /gene="nadA"
                     /locus_tag="OO244_002914"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5664663.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4140 a         4327 c         4099 g         3861 t
ORIGIN      
        1 ggacccaatg cccgttaata aagccctgca actcattgag atgctgaatg aacaccacat
       61 tcacggtctg atgtatgtcg atgatgcaat ggtctatgag cacccgaccg ggcatgtcat
      121 tcgcacgtct aactgggcgc aaaccctgcc gccggaacag cgtccgactt tcacacaagt
      181 cgcttctctg gctgaaacga cgcaacaagt taacgccgta tggaagttcg ccctgacgca
      241 cgatgacctg ccgcaattgc agcattttgg taagcatgtc gaacatgaac tgggactgga
      301 gtgtgaatgg tcctggcacg atcaggttga tattgcacac ggcggcaaca gcaaaggtaa
      361 acgtttgacg aaatgggttg aggcgcaagg ttggtcgatg gaaaacgtcg tggcattcgg
      421 cgataacttt aatgatatca gtatgctgga agccgctggt acaggcgtgg cgatgggcaa
      481 cgccgatgac gcggtaaaag cgcgcgccaa cattgtgatt ggtgataaca ccaccgacag
      541 cattgcccag ttcatttata gccacctgat ttaatcaggc ggttatcgac acacttttaa
      601 tttgcgcgta cagccacagg ccaggtttga tcgccagttc atccctggcc cacgggctga
      661 tacgcgccca cagcgtttta ccgccgactt ccagctccac ttccacctgg ccgttgtcgt
      721 cataactatt aacaactttt gcccgcaata cgttacgaat gctggtttgc tgcggcggtt
      781 gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg tagcgcagct tgcagcggtt
      841 cgcccagctt attgacccac aaatgctgat cgcccagcgc cagcgcggtc atcgcgtaat
      901 gcggatgatg ctcaagcacc gtcactttca gaatgctact ttgttgctct ttcggcagcc
      961 acggattcat cacgctactg ccccacactt cctccagcgc gccaaaggct ttcacctgac
     1021 cgttttccag taccatcact ctgtctgcca gatggaggat ctcatccagc gaatggctga
     1081 catacaacat cggaatgttg atttcccgtg tcagccgttg cagataaggc aacagttcgc
     1141 gtttacgcgg aatatccagt gacgccagcg gttcatccag caacagcaat tccggtgctg
     1201 tcagcaaagc ccgaccaatc gccacgcgct gtttttcgcc tccggacagg ctgcctggta
     1261 aacggtcaag caacggttca atgcctaaaa gcgccaccag cttatcgaac tgatcgacca
     1321 tacttttcga catgccgtag cgcagattgc cacgcacttt gtaatgcggg aacaaccgcg
     1381 catcctgaaa aacatagcca acacgacgct tttccggcgt caggcagata cctttttcgg
     1441 catcatttag tacccgccca ttgaggacaa tccgcccttt ttgcgggcgc gtcagtccac
     1501 tgatggcgtt aatcagcgaa gtttttccgg caccggagac gccaaagata gcagtgatgc
     1561 cattggcggg cagcgtttca ttaatggtca ggcaatggtt gcccaacgtc tgggaaaaat
     1621 tcagctccag catgattagc gccccgcccg ttcacggctg attctggcca gccattctga
     1681 aatcaacagg gagatcatcg ccagcgcaat agaaataatg cacagtctcg ccgcgccact
     1741 ttccccgccg ggggtctgga tcagggtata catggcagaa ggaatggttc gcgtttcgct
     1801 aggaatgttc gacacaaagg tgatggttgc accaaactca ccgagagaac gagcaaaagc
     1861 cagtaccgta ccaacaataa ttcccggtaa ggtcagcggt aacgtgatag taaagaaaac
     1921 gcgccagcgc ccggcaccta gtgttcttgc ggcctgttcc agtttgacat caaccccttc
     1981 cagcgccaga cgaattgccc gcaccatcag cggaaacgac atgacggcgg cagcgagaac
     2041 cgcgccgcgc cagctaaagg cgaaggtaat accaaaccag tcatacagac gttcaccgat
     2101 aaatccgcgc cgtcccatcg aaactaataa taagtaaccg acgaccacgg gcggtaacac
     2161 cagcggtaga tgcagtacgc tgtcgagcag agctttgccc ggaaacgtgc aacgcaccag
     2221 taaccaggca aaaaagatcc caaacggcag gctaaacagc acagccaggg aagaaacttt
     2281 caggcttaat aaaactgcct gccattctgg atcggtcagt atcattactt ggttgtaaat
     2341 ccgtaacgtt taaagatttc cgctgcctgc ggtcccttca gataatcata aaatgctttc
     2401 actgtcgcat tgttatgccc ttccacaaca gcaaccggat attccacttt tttatgtgaa
     2461 tcttccggga aggtggcaac cacttttacc cctttgctgg caactgcgtc agaaccgtag
     2521 acaatgccca gaggcgcttc gttacgttcg accagcgcca gcgccccacg aacatcttcc
     2581 gctggggcca gtttcggaga gagcgtatcc catgcgccca gtttttgcag tgcttctttt
     2641 gcataaatgc cagcgggaac atgttccgga tcgccaaccg ccaggcgacc gccattcagc
     2701 agcgaagtcc agttagtttt gctgtcgatg gtgaaatctt tctgctcgct ggctttcggt
     2761 gctacaacga ccaggctatt gccgagcagt gtctgacgcg tagcagtatc gatcgctttt
     2821 ttatcaaccg cataatccat ccatttctga tcggcagaaa taaacagatc cgcaggggca
     2881 cccgcttcaa tctgacgggc gagagtagat gacgaagcga aagaagaaac cacatccacg
     2941 cctttctctt ttttatactg cgtagcaatg tcctgcattg cgttagtcag tgatgcggcg
     3001 gcgaacaccg tgattttccc ttcatctgcc agtgcattgc cagcaactgc gaaagagagt
     3061 gccgccccgg caaacaagtt caaccattta cgagccattg gtaacccctt aatgtaacgt
     3121 tatataggcg acaatataac gactaactcg gaattttccc agcagttatt gctaaccttt
     3181 gtgaggtaga taagaaaaaa tatcggcagg aaaagcagga agttgagaaa aagaaaatgc
     3241 ccgactaagc gggcactcag ggaatcaatg attttgtccg ggctggtctt ttttaccaac
     3301 accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga tgatggccat
     3361 cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta acatggtcaa
     3421 ctccagatat agtcatgaga ctattctaac cgctaagcac agaaaagcac tccccttttg
     3481 tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agacttttgc caggacattg
     3541 ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc gccgacccgc
     3601 gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag ggagcgaaag
     3661 atgccggtat cagctataaa agcgcctggg atgccattaa cgagatgaat cagttaagtg
     3721 agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg cggaggcgca gtactgaccc
     3781 gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag caaaaagcct
     3841 ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc gcgatctcac
     3901 gtttttcact gcaaaccagc gcccgtaacc agtggttcgg taccatcacc gcccgcgatc
     3961 atgatgacgt tcaacagcat gttgatgtct tactggctga cggaaaaaca cgcctgaaag
     4021 tcgcaattac cgcacaaagc ggcgcacgtc tggggctgga tgaaggcaaa gaagtgttga
     4081 tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgca caaaacgccg
     4141 acaaccaatt accgggtatt attagtcata ttgagcgcgg cgcagagcag tgcgaagtat
     4201 taatggcgct acccgacggg caaacactgt gcgccacagt gccggtaaat gaagcgactt
     4261 ctcttcagca agggcagaat gtcacggcct actttaatgc cgacagcgtg attatcgcca
     4321 cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca gtaatcgctg
     4381 cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt tcgtcttagc
     4441 gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag ttgggcgttt
     4501 gtcggttcga atggaagtgg aaagtcggcg ctggcccgcg cgctggcggg ggaacttccg
     4561 cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc cttcgagcaa
     4621 ttgcaaaagc tcgtcagcga cgaatggcaa cggaataaca ccgatatgct cggccctagc
     4681 gaagattaca ccggacgcac tacggctgag attattcagg atgaagtaaa ggatgcaccg
     4741 cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg acgctttaaa
     4801 tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat gtcggagcct
     4861 gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg tcagcagctg
     4921 gctgagcgac tcgcctcgtt acatcagtcc ggtattactt tggtactggt gctcaatcgc
     4981 ttcgataaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg tacgttagcg
     5041 gaaactggcg ctaaagagga actgctccag caagcactcg tcgcgcaact tgcgcatagt
     5101 gaacagcttg aaggtgtgca actgccggag ccggatgaac cttcagcacg tcacgcctta
     5161 cccgccaacg aatcgcgcat tgtgctgaac aatggcgtgg tttcttataa tgatcgcccc
     5221 attcttaata accttagctg gcaggtgaat ccaggcgaac actggcaaat tgtcgggcca
     5281 aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc gcaaggttac
     5341 agcaacgatt tgacgctttt cggccgacgt cgcggcagca gcgaaaccat ctgggacatc
     5401 aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt cagcactacc
     5461 gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca ggtcgtttcg
     5521 gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga taaacacacg
     5581 gctgacgctc cgttccatag tctttcctgg ggacagcagc gtttggcgct gatcgtccgc
     5641 gcactggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg gcttgatccg
     5701 ctcaatcgcc aacttatccg ccgttttgtt gatgtgctga ttagcgaagg tgaaacgcaa
     5761 ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca tcgccttgag
     5821 ttcgtgccgg acggtggact ctatcgctat gtgctgacaa aaatatattg agtcggtagt
     5881 gctgaccttg ccggaggcgg cctaagcgca ctctccggcc aacggttcga cgcatgcagg
     5941 catgaaaccg cttctttttt cagataaaaa gcgcaatcat tcataaaccc tctgttttat
     6001 aatcacttaa tcgcgcataa aaaacggcta aattcttgtg taaacgattc cactaattta
     6061 ttccatgtca cacttttcgc atctttgtta tgctatggtt atttcatacc ataagcctaa
     6121 tggagcgaat tatgagagtt ctggttaccg gtggtagcgg ttacattgga agtcatacct
     6181 gcgtgcaatt actgcaaaac ggtcatgatg tcatcattct tgataacctc tgtaacagta
     6241 agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa acatccaacg tttgttgaag
     6301 gcgatattcg taacgaagcg ttgatgaccg agatcctgca cgatcacgct atcgacaccg
     6361 tgatccactt cgccgggctg aaagccgtcg ccgaatcggt acaaaaaccg ctggaatatt
     6421 acgacaacaa tgtcaacggc actctgcgcc tgattagcgc catgcgcgcc gctaacgtca
     6481 aaaactttat ttttagctcc tccgccaccg tttatggcga tcagccccaa attccatacg
     6541 ttgaaagctt cccgaccggc acaccgcaaa gcccttatgg caaaagcaaa ttgatggtgg
     6601 aacagatcct caccgacctg caaaaagccc agccggactg gagcattgcc ctgctgcgct
     6661 acttcaaccc ggttggcgcg catccgtcgg gcgatatggg cgaagatccg caaggcattc
     6721 cgaataacct gatgccatac atcgcccaag ttgctgtagg ccgtcgcgac tcgctggcga
     6781 tttttggtaa cgattatccg accgaagatg gtactggcgt acgcgattac atccacgtaa
     6841 tggatctggc ggacggtcac gtcgtggcga tggaaaaact ggcgaacaag ccaggcgtac
     6901 acatctacaa cctcggtgct ggcgtaggca gcagcgtgct ggacgtggtt aatgccttca
     6961 gcaaagcctg cggcaaaccg gttaactatc attttgcacc gcgtcgcgaa ggcgaccttc
     7021 cggcctactg ggcggacgcc agcaaagccg accgtgaact gaactggcgc gtaacgcgca
     7081 cactcgatga aatggcgcag gacacctggc actggcagtc acgccatcca cagggatatc
     7141 ccgattaagg aacgaccatg acgcaattta atcccgttga tcatccacat cgccgctaca
     7201 acccgctcac cgggcaatgg attctggttt caccgcaccg cgctaagcgc ccctggcagg
     7261 gggcgcagga aacgccagcc aaacaggtgt tacctgcgca cgatccagat tgcttcctct
     7321 gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga ttacaccggg acttacgttt
     7381 tcactaatga ctttgcggcc ttgatgtctg acacgccaga tgcgccagaa agcaacgatc
     7441 ctttgatgcg ttgccagagc gcgcgcggta ccagccgggt gatctgcttt tcaccggatc
     7501 acagtaaaac gttgccagaa ctgagcgttg cggcattgac ggaaatcgtc aaaacctggc
     7561 aggagcaaac cgcagagctg ggaaaaacgt acccgtgggt gcaggtcttt gaaaacaaag
     7621 gtgcggcgat gggctgctct aacccgcatc cgcacggaca gatttgggca aatagcttcc
     7681 tgcctaacga agctgagtgc gaagaccgcc tgcaaaaaga atattttgcc gaacagaaat
     7741 caccaatgct ggtggattat gttcagcgcg agctggcaga cggtagccgt accgttgtcg
     7801 aaaccgaaca ctggttagcc gttgtgcctt actgggctgc ctggccgttc gaaacgctac
     7861 tgctgcccaa agcccacgtt ttgcggatca ccgatttgac cgacgcccag cgcagcgatt
     7921 tggcgctggc gttgaaaaag ctgaccagtc gttatgacaa cctcttccag tgctccttcc
     7981 cctactctat gggctggcac ggcgcaccat ttaatggcga agagaatcaa cactggcagc
     8041 tgcacgcgca cttttatccg cctctgctgc gctccgccac cgtacgtaaa tttatggttg
     8101 gttatgaaat gctggcagag acccagcgag acctgaccgc agaacaggca gcagagcgtt
     8161 tgcgcgcagt cagcgatatc cattttcgcg aatccggagt gtaagaaatg agtctgaaag
     8221 aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac accattcagg
     8281 cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt ttcgttctgc
     8341 cctgcgcgat tgattatcaa accgtgatca gctgtgcacc acgcgatgac cgtaaagttc
     8401 gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcaat gcgcccattg
     8461 tcgcgcatga aaactatcaa tgggcgaact acgttcgtgg cgtggtgaaa catctgcaac
     8521 tgcgtaacaa cagcttcggc ggtgtggaca tggtgatcag cggcaatgtg ccgcagggtg
     8581 ccgggttaag ttcttccgct tcactggaag tcgcggtcgg aaccgtattg cagcagcttt
     8641 atcatctgcc gctggacggc gcacaaatcg cgcttaacgg tcaggaagca gaaaaccagt
     8701 ttgttggctg taactgcggg atcatggatc agctaatttc cgcactcggc aagaaagatc
     8761 atgccttgct gattgactgc cgctcactgg ggactaaagc agtttccatg ccgaaaggtg
     8821 tggctgtcgt catcatcaac agtaacttca aacgtaccct ggttggcagc gaatacaaca
     8881 cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca gcagccagct ctgcgcgatg
     8941 tcaccattga agagttcaac gctgttgcac atgagctgga cccaatcgtg gcgaaacgcg
     9001 tgcgtcatat cctgactgaa aacgcccgca ccgttgaagc tgccagcgcg ctggagcaag
     9061 gcgacctgaa acgtatgggc gagttgatgg ccgagtctca tgcctctatg cgcgatgatt
     9121 tcgaaatcac cgtgccgcaa attgacactc tggtagaaat cgtcaaagct gtgattggcg
     9181 acaaaggtgg cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc gcgctgatcc
     9241 cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa gcaaaaacag
     9301 gtattaaaga gactttttac gtttgtaaac catcacaagg agcaggacag tgctgaacga
     9361 aactcccgca ctggcacccg atggtcagcc gtaccgactg ttaactttgc gtaacaacgc
     9421 agggatggta gtcacgctga tggactgggg tgcgacttta ctttccgccc gtattccgct
     9481 ttccgatggc agcgtccgcg aggcgctgct cggctgtgcc agtccggaat gctatcagga
     9541 tcaggctgcg tttctggggg cctctattgg tcgttatgcc aaccgcatcg ccaatagtcg
     9601 ctataccttt gacggtgaaa ccgtgacgct ttcgccaagc cagggcgtta accagctgca
     9661 cggcggcccg gaagggttcg acaaacgtcg ctggcagatt gtgaaccaga acgatcgtca
     9721 ggtgctgttt gccctgagtt cagatgatgg cgatcagggc ttcccgggta atctcggcgc
     9781 gacggtgcaa tatcgtctga ccgacgataa ccgtatctcc attacttatc gcgccacagt
     9841 tgataaacct tgcccggtga atatgactaa tcacgtctat ttcaatcttg acggcgagca
     9901 gtctgacgtg cgcaatcaca agttgcagat tctggcggac gaatatcttc cggttgatga
     9961 aggcggcatt ccgcacgacg gcttgaaatc tgtcgccgga acgtcttttg atttccgcag
    10021 cgccaaaatc atcgccagtg agtttcttgc cgacgacgat cagcgcaaag tgaaaggtta
    10081 cgatcacgca ttcttgttac aggccaaagg cgatggcaag aaagtggcgg cgcatgtctg
    10141 gtcagcagat gaaaaattgc agctgaaggt ctacaccacc gctccggctc ttcaattcta
    10201 ctccggcaac ttcctcggcg gcacaccgtc gcggggaacc gaaccttacg ccgactggca
    10261 agggctggcg ttggaaagcg agtttctgcc ggacagcccg aaccaccctg aatggccgca
    10321 accggactgc ttcctgcgtc ctggcgaaga gtattccagc ctgacggaat atcagtttat
    10381 tgctgagtaa tgttaagccc tcgtcatgag ggctttatct tcatattgtt caaatcacca
    10441 gcaaacaccg acatatttgc aactcaatat tcacaacaac cttacactgc gccactattt
    10501 tcgctatggt tatgcgtaag cattgctgtt gcttcgtcac ggcaatataa tgagaattat
    10561 tatcattaaa agatgatttg aggagtaagc atatggctgt aactaagctg gttctggttc
    10621 gtcatggcga aagtcagtgg aacaaagaaa accgtttcac cggttggtac gacgtggatc
    10681 tgtctgagaa aggcgtaagc gaagcaaaag cagcaggtaa gctgctgaaa gaggaaggtt
    10741 acagctttga ctttgcttac acttctgtgc tgaaacgcgc tatccatacc ctgtggaatg
    10801 tgctggacga actggatcag gcatggctgc ccgttgagaa atcctggaaa ctgaacgaac
    10861 gtcactacgg tgcgttgcag ggtctgaaca aagcggaaac tgctgaaaag tatggcgacg
    10921 agcaggtgaa acagtggcgt cgtggttttg cagtgactcc gccggaactg actaaagatg
    10981 atgagcgtta tccgggtcac gatccgcgtt acgcgaaact gagcgagaaa gaattgccgc
    11041 tgacggaaag cctggcgctg accattgacc gcgtgatccc ttactggaat gaaaccattc
    11101 tgccgcgtat gaagagcggt gagcgcgtaa tcatcgccgc acacggtaac tccctgcgtg
    11161 cgctggtgaa atatcttgat aacatgagcg aagaagagat tcttgagctg aatatcccga
    11221 ctggcgtgcc gctggtgtat gagttcgacg agaatttcaa accgctgaaa cgctattatc
    11281 tgggtaatgc tgacgagatc gcggcgaaag cagcagccgt tgcgaaccag ggtaaagcga
    11341 agtaaacgtc attcgtttaa aaaatgagaa agccgactgc aagtgagtcg gcttttttgt
    11401 ttgtcgttgc taacaatgga gcacattgcc tgatgcgacg ctgcgcgtct tatcaggcct
    11461 gtggtgattc atcggatacg ccactctgac ggcgcatccg acaattaaac cttacccgcg
    11521 acgcgctttt actgcattca ccagttgacg taacagagca tcggtatctt cccagccaat
    11581 gcaggcatcg gtgatgctct taccgtaggc cagcggctcc ccgctatcga ggctctgatt
    11641 gccttccacc agatggcttt ccaccatcac gccaataatg gccttttcgc caccggcaat
    11701 ctgctggcaa acgtcagcac aaacatccat ctgctttttg aattgtttgg acgaattagc
    11761 atggctgaaa tcgatcatca cctgagctgg cagacctgct ttgttcagcc cttctttcac
    11821 ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa tgatatggca
    11881 atcgccgtta ccactggtat tcacaatcgc cgaatgcccc catttagtta cggacaggaa
    11941 gcagtgcggc gcaccggcgg cattaatggc atcgatagcc actttaatcg taccgtctgt
    12001 gccattttta aaacctaccg gacaagaaag accagacgcc agttcgcggt gcacctgcga
    12061 ttccgtggta cgtgcaccaa ttgcgcccca gctcatcagg tcagcgagat actgaggagt
    12121 gatcatatcg agaaactcac ccgccgctgg cagaccgctg tcgttaatat caagcagcaa
    12181 tttacgggct atacgcagac cgtcgttgat ctggaagctg ttatccatat gcggatcgtt
    12241 aatcaaccct ttccagccca ccgtggtacg cggcttttca aaatagacgc gcattacgat
    12301 ttccagctca tctttcagct cttcacgcag cgccagcaag cgagtggcat actctttagc
    12361 cgcgacagga tcatgaattg agcacgggcc aatcaccacc aacaggcgat catcattacc
    12421 tttcaggatc ttatggatcg cttttcgggc atgggcaacc gtattcgcgg cattttcagt
    12481 tgcggggaat ttttccagca atgcgacagg aggaagtaac tctttgattt ctttgatgcg
    12541 taaatcgtcg ttctgataat tcatgtctgt tccagtgttg ccatacttat cttagtgaat
    12601 gcaatacttc caatctatat cttccgtcag aatgtgtaaa cggggtttta cactatgaac
    12661 ggattaatcc tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg agatctctcc
    12721 tacaatttct aactgtaact cctttgtttg ttaattattt caagattctc tgctgcgttt
    12781 cataaactgg ctgaaatctt aaaccaatgc cttatattca cctgcaaatg cactgttgga
    12841 agaggttatc cgacataacg accataacag gagcatccta tgaaaatgac aaaactggcc
    12901 acattatttc tgactgccac tctaagcctt gccagcggtg ccgcactggc tgccgatagc
    12961 ggagcgcaaa ctaataacgg ccaggcaaac gccgcagctg atgcgggcca ggtagcccct
    13021 gacgcccgtg aaaatgtcgc gccaaataac gtcgacaata acggggtaaa taccggttct
    13081 ggcggcacaa tgctgcattc ggatggttct tcaatgaaca atgacggaat gaccaaagat
    13141 gaagagcaca agaacaccat gtgcaaagat ggtcgatgtc cggatatcaa taaaaaggta
    13201 caaaccggtg atggcatcaa taatgatgtc gataccaaaa ccgacggtac cacgcagtaa
    13261 ccctgcaaaa tagccaataa tgggagagcg gagtctctcc cttttaattt ttcacttcta
    13321 aaagcagtat gatgtcataa agttaagtga gaataattaa gggatgactc gtcatggcgc
    13381 actcacactc acacacgtcc tcacacctgc cagaagataa taatgctcgt cgcttgttgt
    13441 atgctttcgg cgtgactgcc gggtttatgc tggtagaagt cgttggtggt tttctttctg
    13501 gttctctggc attgctggcc gatgcgggtc atatgttgac cgatactgcc gccctgcttt
    13561 tcgccctact cgccgtgcaa ttttcacgtc gtcctcccac cattcgccac actttcggtt
    13621 ggctaagact gaccacgctc gcggcgtttg tgaatgccat cgccctcgtg gtaattacca
    13681 ttttgattgt ctgggaggcg atagaacgct tccgcacgcc gcgtccggtc gagggaggca
    13741 tgatgatggc aattgccgtg gctgggttgc tggcaaatat actttctttc tggttacttc
    13801 atcacggcag cgaagagaaa aacctcaacg tacgagcagc ggcactgcat gtgctcgggg
    13861 acctgctggg ttcggttggg gctattattg ccgcgttgat tattatctgg actggctgga
    13921 cacctgctga ccccattctc tcgatactgg tgtcgcttct ggttctgcgt agcgcgtggc
    13981 gactgttgaa agatagcgtg aatgaattac ttgaaggtgc accggtatcg ctggatatcg
    14041 ctgaactgaa gcgtcgtatg tgccgggaaa tcccggaagt ccgcaatgtg caccatgtac
    14101 atgtatggat ggtaggcgag aagccggtga tgacgttgca tgtgcaggtg atcccgccac
    14161 acgatcacga tgccttgttg gatcagatcc aacactacct gatggatcac tatcagattg
    14221 agcacaccac cattcagatg gaatatcaac cttgtcatag gccagactgc cacctgaatg
    14281 aaggggtgtc tggccattca catcatcacc attaatggga cagcgcgcgt gagcctcttt
    14341 cacgtgcgct gttgatccac atccggctgc cgttgagcgc aataaaggtc aggatgatgt
    14401 actccagaga catggcgtaa acgccctgaa gtgcaaaaat aacgacgcta atcacgttaa
    14461 taatcaccca caacagccag ttttccacat acttacgcgt catcagaatc attgccacga
    14521 tagataacac catcatgcat gaatcccaga acgggaaagc gtccggttgc agttcaggca
    14581 tcacaacctg taatcctaat gcttgcatga tcatgactgc cacgcgggtc aaaaatgcaa
    14641 acaccggatt gataaatacc gtcatcagac caatcgaaac aacgcaaacc gccaaccagc
    14701 tgagtgcctt cggcaatggc aaccagcgaa ttttcaactc cgcctcgttc tgactggttt
    14761 gtcgcgacca cgcataccag ccgtaaatat tcgcggcaaa gaaaaacacc tgtaatagca
    14821 ggctggcata cagctgaatc tgaaagaaaa taatgccaaa caaggtaacg ttgatcaggc
    14881 caaagaagta gttgctgatc ttctccagac tggcaaggcc aatacacagc aacccggcga
    14941 tcgtgcctac cgcttcgatc catgagagat cataaccgcc cgcccctatt ggtatatgta
    15001 ccaggatatt ctgcacacta aaaaaatcca tctttccccc aaagcgtaac gttacgcctt
    15061 attattcgtt atccacgtag tgtagccgca aaatccagca tacgattgag cggcaccagc
    15121 gccctctctc gcagcctctc atcgacatga acctcgtgat tacttccttc ctgttctaat
    15181 gcctctgcga tagcctgaag gtcattcatg gccatccacg gacaatgcgc gcagctgcgg
    15241 caggttgcgc cctcacccgc ggttggtgct tccagtaact ctttatctgg caccgcctgc
    15301 tgcattttgt agaaaatacc ccgatcggtt gccacaataa gcctctgatg aggcaatgct
    15361 tttgcagcag cgatcagttg actggtggaa ccgaccgcat ccgccatatc gacaatagct
    15421 tgtggtgatt ctggatgcac cagtatggca gcatccgggt attcttcttg caagcgggtt
    15481 aacgcctgag tcttaaattc atcatgcaca atgcaggcac cctgccagca taaaatgtct
    15541 ccacccgtct gtttttgcac gtaacgcccc agatgtttgt cgggtgccca gatgattttt
    15601 tcacccaaac tatcaagatg atcaataagt tcgacggcaa tgcttgaagt taccacccaa
    15661 tctgcgcgcg cttttaccgc agcagaagtg ttggcgtaga cgacgacagt acgatcggga
    15721 tgggcatcgt aaaatgcgtt aaattcttca acagggcagc cgagatccag tgaacactca
    15781 acctgaagtg tcggcatcag aattgttttt tccggactga gaattttggc ggtttctccc
    15841 ataaatctca ccccagcgac taacaaagta gaagcgggat gctttgcacc gaagcgcgcc
    15901 atttccagag aatcagaaat acagccaccg gtttcttctg ccagttgttg aatttcggga
    15961 tcggtatagt agtgggcaac catcaccgca ttacgttctt ttagcagacg ttttatcttc
    16021 tcgcggtaat acgctttttc atcaatgctt aacggcgtcg gcttcggggg gaaaggataa
    16081 atcgccgtgt ctggatcaaa cattacgctc atcttaccat ctcgttttac aggcttaacg
    16141 ttaaaaccga cattagcgta acattcgctg ttatcggtgt aatttgtttt atatgctaaa
    16201 caagatagtc gaatggtggg gaaaagtcac tcgaattacg cctaattcca ccgacgtaga
    16261 gttgcttgca cagtaagctc tgacgatgtc atcaggttta caaagtgaaa ttgaggatgc
    16321 ctgaatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg tgcaggattc
    16381 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagc
//
LOCUS       DAJTOW010000084        16385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTOW010000084 DAJTOW010000000
VERSION     DAJTOW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.084"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO244_002915"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO244_002915"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5664664.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO244_002916"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO244_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5664665.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="OO244_002917"
     CDS             2341..2556
                     /locus_tag="OO244_002917"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664666.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTMVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="OO244_002918"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="OO244_002918"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5664667.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /gene="galP"
                     /locus_tag="OO244_002919"
     CDS             4451..5845
                     /gene="galP"
                     /locus_tag="OO244_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5664668.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="OO244_002920"
     CDS             5922..6419
                     /locus_tag="OO244_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5664669.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="OO244_002921"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="OO244_002921"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5664670.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="OO244_002922"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="OO244_002922"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5664671.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="OO244_002923"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="OO244_002923"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5664672.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9104..9667
                     /locus_tag="OO244_002924"
     CDS             9104..9667
                     /locus_tag="OO244_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5664673.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="OO244_002925"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="OO244_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5664674.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="OO244_002926"
     CDS             complement(10259..11239)
                     /locus_tag="OO244_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5664675.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="OO244_002927"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="OO244_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5664676.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="OO244_002928"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="OO244_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5664677.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="OO244_002929"
     CDS             12542..12832
                     /locus_tag="OO244_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5664678.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="OO244_002930"
     CDS             12840..13433
                     /locus_tag="OO244_002930"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5664679.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="OO244_002931"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="OO244_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5664680.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="OO244_002932"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="OO244_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..>16385
                     /locus_tag="OO244_002933"
     CDS             15884..>16385
                     /locus_tag="OO244_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5664681.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4218 c         4124 g         3985 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctacgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgagtcttc
     2401 accagaacgg tgagacaggt taagcagcag acaatggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccaaggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg ccgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggac tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cggcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ctgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcccttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taactatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact tgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagccta gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggtgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgagca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cttgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tatacaatgg tgctgttatt acgcatctgg atgcagtgtg
    12061 ctcattgtga tttttacacc cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgcc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtgtg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgactatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg tacaaatcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tggcgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggg ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 taaccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgccttg tgttttatta atcaaagcat tcaacaactg aaacaacctg cgcaggaggc
    16381 cagtt
//
LOCUS       DAJTOW010000085        16382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTOW010000085 DAJTOW010000000
VERSION     DAJTOW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.085"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..836
                     /locus_tag="OO244_002934"
     CDS             <1..836
                     /locus_tag="OO244_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5664682.1"
                     /translation="KRFNIFISIPCDWGDDVTTPRRQIYMSNSTTFDDQGFASNNTGL
                     SGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQTGASVS
                     GGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTPYREN
                     HLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPLTFGY
                     EVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESRNYIC
                     Q"
     gene            852..1885
                     /locus_tag="OO244_002935"
                     /pseudo
     CDS             852..1885
                     /locus_tag="OO244_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(1967..2305)
                     /gene="rcnB"
                     /locus_tag="OO244_002936"
     CDS             complement(1967..2305)
                     /gene="rcnB"
                     /locus_tag="OO244_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5664683.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(2524..3354)
                     /gene="rcnA"
                     /locus_tag="OO244_002937"
                     /pseudo
     CDS             complement(2524..3354)
                     /gene="rcnA"
                     /locus_tag="OO244_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            3476..3748
                     /gene="rcnR"
                     /locus_tag="OO244_002938"
     CDS             3476..3748
                     /gene="rcnR"
                     /locus_tag="OO244_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5664684.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      3840..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3971..4759
                     /gene="thiM"
                     /locus_tag="OO244_002939"
     CDS             3971..4759
                     /gene="thiM"
                     /locus_tag="OO244_002939"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5664685.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            4756..5556
                     /gene="thiD"
                     /locus_tag="OO244_002940"
     CDS             4756..5556
                     /gene="thiD"
                     /locus_tag="OO244_002940"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5664686.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            5621..6442
                     /locus_tag="OO244_002941"
     CDS             5621..6442
                     /locus_tag="OO244_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5664687.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            6490..7236
                     /locus_tag="OO244_002942"
     CDS             6490..7236
                     /locus_tag="OO244_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5664688.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(7210..8175)
                     /locus_tag="OO244_002943"
     CDS             complement(7210..8175)
                     /locus_tag="OO244_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5664689.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8172..9175)
                     /locus_tag="OO244_002944"
                     /pseudo
     CDS             complement(8172..9175)
                     /locus_tag="OO244_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(9172..10449)
                     /locus_tag="OO244_002945"
     CDS             complement(9172..10449)
                     /locus_tag="OO244_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664690.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10706..11758
                     /gene="fbaB"
                     /locus_tag="OO244_002946"
     CDS             10706..11758
                     /gene="fbaB"
                     /locus_tag="OO244_002946"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5664691.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12066..12920
                     /gene="gatY"
                     /locus_tag="OO244_002947"
     CDS             12066..12920
                     /gene="gatY"
                     /locus_tag="OO244_002947"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5664692.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            12949..14211
                     /gene="gatZ"
                     /locus_tag="OO244_002948"
     CDS             12949..14211
                     /gene="gatZ"
                     /locus_tag="OO244_002948"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5664693.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            14221..14673
                     /gene="gatA"
                     /locus_tag="OO244_002949"
     CDS             14221..14673
                     /gene="gatA"
                     /locus_tag="OO244_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5664694.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            14704..14988
                     /gene="gatB"
                     /locus_tag="OO244_002950"
     CDS             14704..14988
                     /gene="gatB"
                     /locus_tag="OO244_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5664695.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            14992..16347
                     /locus_tag="OO244_002951"
     CDS             14992..16347
                     /locus_tag="OO244_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5664696.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4241 a         3913 c         4188 g         4040 t
ORIGIN      
        1 agaaacgttt taatattttt atatcaattc cctgtgattg gggtgatgac gttacgacgc
       61 ctcgtcggca aatatatatg tctaactcaa cgacgtttga tgatcagggt tttgcctcaa
      121 ataatacggg attatcagga acagtaggga gtcgggatca gttcaattat ggtgtcaacc
      181 tgagtcatca acatcaggga aatgaaacga cagctggggc gaatttgacc tggaacgcgc
      241 cggttgcgac agtgaatggc agttatagtc agtcgagtac ttatcgacag actggagcca
      301 gtgtttcagg gggcattgtc gcctggtcgg gtggcgttaa tctggcgaac cgtctttccg
      361 aaacgtttgc tgtgatgaat gcgccaggaa ttaaagatgc ttatgtcaat gggcaaaaat
      421 atcgcacaac aaaccgtaat ggagtggtgg tatacgacgg aatgacacct tatcgggaaa
      481 atcacctgat gctggatgtg tcgcaaagcg atagcgaagc agaattacgt ggcaaccgga
      541 aaattgccgc cccttatcgc ggcgcggttg tactggttaa ttttgatacc gatcagcgca
      601 agccctggtt tataaaagcg ttaagagcgg atggtcaacc attaacgttt ggttatgaag
      661 tcaatgatat ccatggtcat aatattggcg ttgtcggcca gggaagccag ttatttattc
      721 gcaccaatga aataccgcca tcggttaatg tagcaattga taagcaacaa ggactttcat
      781 gcacaatcac cttcggtaaa gagattgatg aaagtagaaa ttatatttgc cagtaatagt
      841 caggtggttc tatggaaatt cgcataatgc tatttatatt aatgatgatg gttatgcctg
      901 tgagctatgc ggcatgttat agtgagttat ctgttcagca caatttggtt gttcagggga
      961 attttgcact tactcaaaca caaatagcga catatgagca taattttaat gattcgtcat
     1021 gcgtgagtac aaatactatc acccctatga gcccatcgga tattattgtt ggactttata
     1081 acgataccat taaattaaat ttacattttg aatggaccaa taaaaacaac atcacgttgt
     1141 caaataatca gagcagtttc accagtggtt attcggtaac ggtgacacct gcggccagta
     1201 atgcaaaagt gaatatttct gcggggggcg gtggttcagt gatgattaat ggtgttgcga
     1261 cattatccag tgcttcatca tcgacacgcg ggagtgccgc agtacaattt ctactgtgtt
     1321 tattaggtgg caagtcatgg ggtgcatgtg taaatagcta cagaaatgca ttggcacaaa
     1381 atgcaggtgt ctattccttc aatctgacat tgtcatacaa cccgataacc acaacctgca
     1441 aacaggacga tttattaatt actttagaca gtattcccgt tttacaatta ccagccacag
     1501 gtaacaaagc aacaataaat agtaaaaaaa gggatattat tctgcgttgt aaaaatttat
     1561 taggtcaaca aaatcaaaca tcacggaaaa tgcaggtgta tttatcaagt tctgacttgt
     1621 taaccaacag caacacaata ctgaaaggtg cggaagataa tgcgtaggat ttattcttga
     1681 aagtaatggt tcgccagtca cacttttaaa tatcactaac agcagtaaag gatacacaaa
     1741 tttaaaagaa attgcggcaa agtcaaaact tacagataca acggtttcaa ttccgataac
     1801 agccagttac tacgtctacg atacaaacaa agttaaatct ggcgcactgg aggcaaccgc
     1861 attaatcaac gtgaaatacg actaataaca agcaaaacga gcaagtggct aataataaaa
     1921 aataacaagg tgtctgcatt tccccctcat gatgaggggg ctttttttag cgatgataaa
     1981 aaatctcacc gtcgtaggct ttaatgattt taccgtcggt gtcgctgatc aacacgtacg
     2041 cgccacccat ataggtccag tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc
     2101 actgcttaat gttgtactca tcggtgcggt acatctctgg gacggtatca cctggtttga
     2161 aatgcttaaa gtcagcaatg aaactactta actcatactt agaaattccc gaggtattgg
     2221 tcgaacccgc ggtggctggt gcggcccagg cggcactggt aaccagcaaa agcacaccca
     2281 gcaacatctt atttttaatg gtcataaact ctccactttg gggatctgta atatcagtcg
     2341 catatctttc ctgtcataca atataactga caagcgatat tttgttagtg atcccatgat
     2401 ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc cttagcgagc agagagtcag
     2461 cggaaatagc tgatttaaga atcatcctgg gctgaagata acatgcaagc ctaaaggatt
     2521 gagttatcgc attatgccca tgaagccatg tacgcccata tacacaccga ctaagccaat
     2581 caacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt
     2641 tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag
     2701 gccaatgctg aaactgacga ccagtgttgc gcccagtgtc agggctttca actgaatgca
     2761 tatcaacagc acggtaattg ctgccgggca ggggataaag ccaccggtta agccaaataa
     2821 caaaatttgc cagttgatga cctctctacc atcaaagcgt cgtttaatgt cattggcatg
     2881 ggctcgtgca tgggcatcct gatactcgcc atgttcgtga tggtgatgat gtccttggtc
     2941 gtggtgatgt tcgtgatcgt gatgatggtg atgatgctca taatcatgcc cgtgcatatt
     3001 ctccagccag ttgcgttcgc cgcgctaggt acgccagaac atccagaacg cggtgccaat
     3061 aatgatcact gcggaaatca gctggagcca cggttctgct gattgagcag taaagcgctt
     3121 actgatcacc atcccgccaa aggcaatcaa ccagaccacc gcggtatgcg aaatggttgc
     3181 tgccagtccg agcatcaccg cctgtttaat ggtgcctttg atggcgatga taaacgccgc
     3241 catcatcgtt tttgagtgcc ctggttccag gccatgaagc gcaccaagta agatggcgct
     3301 ggggatgaag aaccaggcgt ttccttgctg aagaagagtt gtaaattcgg tcatgataat
     3361 aattcttagt attaattcgg caatctgatt ctactccccc ccagtacctg atactacccc
     3421 cccagtagat taatagtgct atgatttttc atattcttgt taaccaggtg ttgccatgtc
     3481 tcatacaatc cgtgataaac agaaactgaa agcgcgtgcc agtaagattc agggccaggt
     3541 cgtggcgctc aagaaaatgc tcgacgagcc gcacgaatgc gctgcagttt tacaacagat
     3601 tgctgctatc cgtggagcgg taaacggtct gatgcgggaa gtgattaaag gtcatctgac
     3661 ggaacacatc gttcaccagg gggatgagct aaaacgtgaa gaagatctgg atgtcgttct
     3721 gaaggtgctg gattcatata tcaaataatt tattaacgcg attgtaaaac tgccgttttt
     3781 cctcgtttac aacgggtgcg ctggacatta ccatcctcct ctgcgattta tcatcgcaac
     3841 caaacgactc ggggtgccct tctgcgtgaa ggctgagaaa tacccgtatc acctgatctg
     3901 gataatgcca gcgtagggaa gtcacggacc accaggtcat tgcttcttca cgttatggca
     3961 ggagcaaact atgcaagtcg acctgctgag ttcagcgcaa tctgcgcacg cgttacacct
     4021 ttttcaccaa cattcccctc ttgtgcactg catgaccaat gatgtggtgc aaacctttac
     4081 cgccaatacc ttgctggcgc tcggtgcatc gccagcgatg gttatcgaaa ccgaagaggc
     4141 cagtcagttt gcggctatcg ccagtgcctt gttgattaac gttggcacac tgacgcagcc
     4201 acgcgcacag gcgatgcgtg ctgccgttga gcaagcaaaa agctctcaaa cacgctggac
     4261 gcttgatcca gtagcggttg gtgcgctcga ttatcgccgc catttttgtc atgaactttt
     4321 atcttttaaa ccggcagcga tacgtggtaa tgcttcggaa atcatggcat tagctggcat
     4381 tgctaatggc ggacggggag tggataccac tgacgccgca gctaacgcga tacccgctgc
     4441 acaaacactg gcacgggaaa ctggcgcaat cgtcgtggtc actggcgagg tggattatgt
     4501 taccgatgga catcgtatca ttggtattca cggtggcgat ccgttaatga ccaaagtggt
     4561 aggaactggc tgtgcattat cggcggttgt cgctgcctgc tgtgcgttac caggcgatac
     4621 gctggaaaat gtcgcatctg cctgtcactg gatgaaacaa gccggagaac gcgcagtcgc
     4681 cagaagcgag gggccaggca gttttgttcc acatttcctt gatgcgctct ggcaattgac
     4741 gcaggaggtg caggcatgaa acgaattaac gctctgacga ttgccggtac tgatccgagt
     4801 ggtggtgcgg ggattcaggc cgatcttaaa accttctcgg cacttggcgc ttatggttgc
     4861 tcagttatta ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt
     4921 gagcctgatt ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg aatcgatacc
     4981 actaaaatcg gtatgttggc ggaaaccgat attgttgaag cggtggcaga acggttgcaa
     5041 cgttatcaga tccaaaacgt ggtactcgac accgttatgc tggcaaaaag cggcgacccg
     5101 ctgctttcac cttcggcggt tgctacgctg cgcagtcgat tattgccaca ggtttcatta
     5161 ataacgccaa acttgcccga agctgccgcc ttgctcgacg cgccacacgc gcgcaccgaa
     5221 caggaaatgc tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg
     5281 aaaggtggtc atctggatga tgagcaaagc ccggactggc tgtttacccg cgagggtgaa
     5341 caacggttta ccgcaccgcg cattatgacc aaaaacaccc acggcactgg ttgtacactc
     5401 tctgcggcgt tggctgcact acgcccgcgc catacaaact gggctgacac cgtacaggag
     5461 gcaaaaatct ggctttcatc ggcgttagcc caggccgaca cgctggaagt tggtcacggt
     5521 attggtccgg ttcaccactt ccacgcctgg tggtgagcac cgtactatag ctgctttggt
     5581 tattgtcagc ctttggcact gacattcagg gatgaaacca atgcagctaa gaattaccag
     5641 tcgaaaaaaa ctcaccgctc tattatgcgc tcttgggcta atttccatcg ttgcaattta
     5701 tccgcgtcag acggtgaatt ttttctattc gacagcagtt cagattacag actacatcca
     5761 cttctacggt tatcgcccgg ttaaatcttt cgctattcgc attcctgcca gttacaccat
     5821 tcacggatta gatgtttcgc gctggcagga gcgtatcgac tggcagcggg tggcaaaaat
     5881 gcgcgacaac gatatccgct tacagtttgc ttttattaag gctacggaag gcgaaaagct
     5941 ggtggacccc tatttttcgc gtaactggca attaagccgc gaaaatggcc tgctgcgcgg
     6001 ggcgtatcat tatttctccc cgtcggtatc cgcttcagtt caggcgcgat tatttctgca
     6061 aacggtggat ttctcacaag gcgatttccc tgccgtgctg gacgtagaag aacggggaaa
     6121 attatcggca aaagaattac gcaagcgggt aagtcagtgg ctaaaaatgg tcgaaaaacg
     6181 cacgggaaaa aagccgatta tttactcagg agccgttttt tatcacacca atctggcggg
     6241 ctatttcaat gaatatccgt ggtgggtggc tcactattat caacgtcgtc cggacaatga
     6301 cggcatggcc tggcgcttct ggcagcattc cgaccgtgga caggtagatg gcatcaatgg
     6361 tccggtggat tttaatgtgt ttaatggcac ggggatgagt tgcaggcatt cgttgatggg
     6421 attaaagaaa cgccttaaat aagaagcagt gatatactcc caccagactc cctggcagga
     6481 aaaagacaaa tggaacaagc gcatacccag ctgattgccc aactgaacga gcgtatttta
     6541 gcggcggata acacgccgct ttatattaag tttgccgaaa cggtaaaaaa tgccgtgcgc
     6601 agcggggtgc tggagcatgg caatattttg cccggtgagc gtgatttaag tcagttaacc
     6661 ggcgtttcgc gcattacggt gcgcaaggcg atgcaggcgc tggaagaaga gggcgtggtg
     6721 acgcgttcgc gcggttacgg cacgcagatc aacaacatct tcgaatactc gttgaaagaa
     6781 gcgagaggtt tttctcagca ggtggtattg cgcgggaaaa agcccgatac gctatgggtt
     6841 aataagcgtg tcgtgaaatg tcccgaagaa gttgcgcagc agctggcggt cgaagcggga
     6901 agtgatgtct ttttgcttaa gcgtattcgc tatgtcgatg aagaagcggt atcgattgag
     6961 gaatcgtggg ttccggcgca tttaattcat gatgttgatg ccatcggaat ttctctttat
     7021 gactatttcc gcagccagca catttaccca caacgtacgc gttcccgcgt tagcgcccgg
     7081 atgccggatg ccgagtttca gtcacatatt cagttagata gcaaaatacc ggtgctggtg
     7141 atcaagcaag tggcgcttga ccaacagcaa cggcctattg agtacagcat tagccactgt
     7201 cgcagcgatc tatacgtttt tgtgtgcgag gagtagttcc tcgcgcgttg gcgcacaatc
     7261 accgccccga tgcccgacaa cccacgacgc cactgcattg cccagtaata cggcatccgc
     7321 cagtggcaga ccagaggcca gcccggcaag tacgccacct gcatgactgt cgcccgcccc
     7381 aatggtgtct acaacttgcg ttggaaatgc cggaatgcag ccagaagcgt cgttgctgaa
     7441 ataccatgcg ccttctttat cgagacgaac gatcaacggc gcggcaaatt tctcctgcca
     7501 ttgcttgcca agtgttgtta tctctgcgga taaagcaaaa cgttcggcgg caatctcagc
     7561 ctcttgacga ttgagtgaca ctaaaggtcg acaggccatg atccgcgcca gtaatgcgtc
     7621 cgggatatcg ccaatacgtg ggcaaaaatc gataaacggc gtcacgtctt gcagcttttc
     7681 cagccattcc actaacaatt cgccgcaggg cgaggccagt tgataaccgg aaaaatagag
     7741 caggttgcca ggcgcaacgg ttaatcgtgc caaccattgg cgattccatt gattttccac
     7801 accgctgaat gacataaaag tgcgttcgcc atccggctca accagtgcca gacaccagcc
     7861 gttatcgctt tcggcgttat cgatcaggct gattaacccc tctttcgcca tccggttgcg
     7921 aataatctcc gcccacacgc cctgaccgag cggcaaggca ttacccgctt cgatgccgag
     7981 gcgctttaac gccacggcaa tattcagtgc gcagccgcca acgttaacgc tctgctgttt
     8041 cagttcgata tcgcacccac gccaggggag ggcataagcg tcggcgatca cgtcaatgac
     8101 cgccgcgccg acgaccatca ccggctgacg cgtggttaat tcaggcagca gtgtgtgtaa
     8161 tcgagcgccg ctcataccgc ctcccgttgt tgacgatatt tcgccagcgc tgtggcatag
     8221 cggttgaaat caagctgatt taccgcatcc agctccgcct ttaacgcagg atcgacagcg
     8281 ttaatgccat gcaacgcgcc gcaaatcgcc gtcgccatag cacctatggt gtctgtgtcg
     8341 ccgccaagat tggcgcacag gacggcgcag cggtttgggt cggtttgtgc cagttcaact
     8401 agcgcaatgg cgcacggaac ggactcaata gtgctggtgc ctgcgccaac gacctggtaa
     8461 agctgttcgc tggcggattc ggtgccgtcg gcattacgca caattttcag cgcaatctcc
     8521 agacgtgccg ccagtgaggc gctgaacgtg gtggtgcgtt tttgctgtgc atgtcgcgca
     8581 attgaaggca atgaatcaac aatcgctgac cagcgttctc cgtcaatggc acgagaaatc
     8641 gcccatgcga tgactaccgc gcctgcaacc gccagatcgg atttatgggt cgggctggac
     8701 gccagcgcca catcatcaat aaaggaatca acatcacgcg ctggaagcaa acaacctaat
     8761 ggcgagacgc gcatcgctgc gccgttggtt acgccgttgt tttccagttc agcaacgggt
     8821 ttaccgtcgc gaatggcgtt aagcgcaatc ttcgaggtcg gacctagtac gtttttgtta
     8881 aaggcgtcga aacgcaacgc ccagtcgaga atattacgcc caatcagatt cggatcgatc
     8941 ttgccttcac attccagtaa cgcatccgcc agacacaggc catcgaggta tcgtcggtga
     9001 attcggcgcg gttaaaataa caggccgcgt tattctcctt tggtccaggc agaaaacggt
     9061 caatccagcc aaagtgtgct ttaacgcggc tgcgtggcca aagctcggag ggcatcccca
     9121 tcgcatcccc taacgcctgc ccataaagag caccgagaat acgttctgtt ttcatttaac
     9181 ttccccttgt gtcaacgcaa tatctcgatc atcgaccttg atagccgtaa tttcgttgtc
     9241 ggattcgcgg aaaaaaatca tgaacagcac ggcgataatg gcaatcatca ccgcgccgaa
     9301 agtccacatc ccggaccagt tgaaagtcag tccgtttacc ggttcctgat aagcgaacat
     9361 cttttccatc atcacaccgc caagacgata gccgagcaaa ctgccgaagc cctggcagca
     9421 gagcgtgatc agcccctgcg cagcggtacg catatgcacg ggggcttttt tatcgacata
     9481 gatgtaagcg gtaacgtagt aaaaatcgta acttacgccg tgaagcaaaa taccgaggaa
     9541 cagtaacgcg taggtgaaat attcatccgc actaccgtaa ataaagaagc catagcagat
     9601 cgcagcggtg accagaccaa gcaataatac ctttttgata ccaaagcgtt tagtgaaaaa
     9661 cggcaatgcc agcataaaga agatttcaga gaactggccg agcgtcatcc agccggtggc
     9721 gtttttcatg ccaacttcgg tcagataacc gttggcaaag atgtaataga acgccagtgg
     9781 catcgcaaac aggaatgaac agaagaaaaa gacgaggaag tttttatcgc gcagcaggat
     9841 cagcgcatcc aggccgagca tgactttaat gtccattttg ccggtgcttt ttggcggcgt
     9901 gtcgggcagg aaaaacgcaa acacaccgag cagagcagaa cttccggcgg taatcagcag
     9961 cgggatgtta gtcggtgaga tatcggcata ccccagcatt tgcggcaaga aaccacatgc
    10021 cagaccggag gcaatccagc cgatagtgcc catcacacga atgcgtggga aatcacgctc
    10081 cacatccggc acgttggcaa aagcgatgct gttagtcagc gcaatggtcg gcatataggt
    10141 tagcgagtag gccagcagta acggaaagaa cccggcaaaa gtggtctgtt gcgcagcgaa
    10201 atacatcagc acagcaccag cgaacatcaa taccgccagc actttctgcg ccgagaaaaa
    10261 gcggtcagtg atggagccaa ccagaattgg cgacaggatc gccgcaatgg ctgtgcaggc
    10321 atacgaccag ccaatttctc cggcactaaa accgctttta cttaaccaca accacaatgg
    10381 cacaaaccac gcgccccaga taaaccattc aacaaacatc atgaacgaca gctttgctgt
    10441 tgttttcatc ttttaattcc ttcatgacag gtaatgtaag gtacgcgatg acaataccac
    10501 ataataatgc cttttgaata cctttgcggt gaaaacttga ccgcgctaac attttttctg
    10561 atgaatcgag ccaacagaaa acgctgaaaa aacatccaaa agatggaaaa actcgcccgt
    10621 aacgactagg attatgattg cccgcaaaag gcgggccagg acaatccata gccgatatcc
    10681 aatcggaatt tacgggagca tagtaatgac agatattgcg cagttgcttg gcaaagacgc
    10741 cgacaacctt ttacagcacc gttgtatgac tattccttct gaccagcttt atctccccgg
    10801 acatgactac gtagaccgcg tgatgattga caataatcgc ccgccagcgg tgttacgtaa
    10861 tatgcagacg ttgtacaaca ccgggcgtct ggctggcaca ggatatcttt ctattctgcc
    10921 ggttgaccag ggcgttgagc actctgccgg agcttcattt gctgctaacc cgctctactt
    10981 tgacccgaaa aacattgttg aactggcgat cgaagcgggc tgtaactgtg tggcatcaac
    11041 ttacggcgtg ttggtgtcgg tatcgcggcg ctatgcgcat cgcattccat ttctcgtcaa
    11101 acttaatcac aacgagacgc taagttaccc gaatacctac gatcaaacgc tgtatgccag
    11161 cgtggagcag gccttcaaca tgggcgcggt ggcggttggt gcgactatct attttggctc
    11221 ggaagagtca cgtcgccaga ttgaagaaat ttctgcggcc tttgaacgcg cgcacgagct
    11281 gggcatggtg acagtgctgt gggcctattt gcgaaactcc gcctttaaga aagatggcgt
    11341 tgattaccat gtttccgccg acctgactgg tcaggcgaac catctggcgg caaccatagg
    11401 tgcagatatc gtcaaacaaa aaatggcgga aaataacggc ggctataaag cgattaatta
    11461 cggttatacc gacgatcgcg tgtacagcaa gttaaccagc gaaaacccga ttgatctggt
    11521 gcgttatcag ttagctaact gctatatggg ccgggctggg ttgataaact ccggcggtgc
    11581 tgcaggcggt gaaactgacc tcagcgatgc agtgcgtact gcggttatca acaaacgcgc
    11641 aggcggaatg gggctgattc ttggacgtaa ggcgttcaag aaatcgatgg ctgacggcgt
    11701 gaaactgatt aatgccgtgc aggacgttta tctcgatagc aaaattacta ttgcctgata
    11761 cgcctcatac gaacagcacc accccgtagg acggataaga cgtttacact gcatccggca
    11821 acctatgcct gatgcgacgc ggaagcgtct tatcatgcct acatagcact gccacgtatg
    11881 tttacaccgc atccggcata aaaacacgcg cactttgcta cggcttccct atcgggaggc
    11941 cgtttttttg cctttcactc ctcgaacaat tttcatattg ttgtttttgt gatcattatc
    12001 tcgacattta aaagcaaata atttcattat attttgaaat cgaaagcaaa cgacaagata
    12061 tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg
    12121 cggttccggc attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg
    12181 ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta
    12241 cagaaaatct gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa
    12301 ttcatctcga ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc
    12361 gctcagtcat gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag
    12421 aggtggtgga tttttgccat cgctttgatg tcagcgtcga ggcggagctg gggcaacttg
    12481 gcggccagga agatgatgtg caagtcaatg aagtcgatgc gttgtacacc aaccccgcac
    12541 aggcgcgtga atttgccgag gcaaccggga ttgattccct ggcggtcgcc atcggcacgg
    12601 ctcacggtat gtatgccagc gcaccggtgc ttgatttttc tcgactggag aacattcgcc
    12661 agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg attatcgact aaggatattc
    12721 agcaaaccat caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct
    12781 tcttgcagtc gttaaaaaat tacctgaccg agcaccccga agcgaccgat ccccgggatt
    12841 atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg
    12901 gctgcgaggg cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta
    12961 attgcccggc ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg
    13021 ttggttatcg aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa
    13081 gcaacgtcaa accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt
    13141 cgcgaatttg tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc
    13201 ggcggcgatc atctgggacc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa
    13261 aaatccgtcg agctggtaaa agcatatgtt cgtgctggct tcagtaaaat tcatcttgat
    13321 gcgtcaatgt cctgcgcggg ggataccata ccgttagcac cagaaacggt tgcggaacga
    13381 gctgctgtgc tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg
    13441 agctatgtca ttggcactga agttccggtt ccgggcggtg aggccagcgc cattcagtca
    13501 gtacacatca cccatgttga agatgccgcc aatactttac gtacgcatca aaaggccttt
    13561 attgcccgtg ggctgacaga ggcgttaaca cgtgtgattg ccatcgtggt gcagccgggt
    13621 gtggaatttg atcacagcaa tattatccat tatcagccgc aggaagcgca ggcgctggcg
    13681 caatggatag aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg
    13741 acggcttatt gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta
    13801 acctttgctt tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc
    13861 cccgaaaatc gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa
    13921 tactggaaaa aatattatcg cacgggtttt aacgattcat tactggatat tcgttacagc
    13981 ctgtcggatc gtattcgtta ttactggccg catagtcgga ttaaaaatag cgttgaaacg
    14041 atgatggtga atctgcaagg cgtggacatc ccactgggca tgattagtca gtatcttccc
    14101 aaacaatttg aacgcattca gtccggggaa ttatcagcaa tgccgcatca gcttattatg
    14161 aataaaattt atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat
    14221 atgactaacc tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc
    14281 tatatcggta atgagatgct cgcgaaaggt gtggttcatg atacctggcc acaggcatta
    14341 attgccagag aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata
    14401 ccgcattgtg aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat
    14461 aaagttcatt ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt ggttattgcg
    14521 ttgattgtgg aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta
    14581 caacagcccg atatcgtcga gacactaatt actcttcctg aaactcagtt aaaggaatac
    14641 ttcacaaagt atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta
    14701 tttatgaaac gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg
    14761 gcggaagaaa ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt
    14821 cgggttaatg aaatagaaac ctatatggat ggtgtgcatt tgatatgtac cactgccagg
    14881 gtggatcgta gttttggcga tattccgtta gttcacggca tgccttttgt ttctggtgtc
    14941 ggtatcgaag cattacaaaa taaaattctg actatcttac agggatgacc tatgttttca
    15001 gaagtcatgc gttatattct cgacctcggc cctacagtga tgctgccggt tgtcatcatt
    15061 attttttcta aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc
    15121 gggattggct ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg
    15181 gcggcgaaag cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg
    15241 ccgggctctt caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg
    15301 atattggtta acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc
    15361 tggaatatct ggcatatgac cttcaccggc gcgttgctgc acctggcaac cggttcatgg
    15421 atgataggga tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg
    15481 tttgcccgcg atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg
    15541 tcggcgtata tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc
    15601 gttaaccgaa ttaaatttag cgccgacgat attcagcgca aatttggccc ttttggcgaa
    15661 cctgtcaccg tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc
    15721 aaaggtgtat tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacggatg
    15781 attaaaccca tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag
    15841 gcgaagttcg gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat
    15901 acggcggtgg tatcggcaag cctgattttt attccgctca ccattttaat tgctgtttgt
    15961 gttccgggta atcaggtact gccgtttggc gatcttgcca ctatcggctt cttcgtggcg
    16021 atggcggtcg cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt
    16081 atgagcatca ccctgtggat cgcgacgcaa actattggtt tgcacaccca accggcggct
    16141 aatgctgggg cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc
    16201 attacctggt tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc
    16261 ggcgcaattt atctgaccgg tattttcatg acctggcgta gagcgcgtgg ttttactaaa
    16321 caagagaaag ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac
    16381 aa
//
LOCUS       DAJTOW010000086        16358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTOW010000086 DAJTOW010000000
VERSION     DAJTOW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.086"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..73)
                     /locus_tag="OO244_002952"
     CDS             complement(<1..73)
                     /locus_tag="OO244_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5664697.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO244_002953"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO244_002953"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5664698.1"
                     /translation="MKSGRFIGVMSGTSLDGVDIVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO244_002954"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO244_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5664699.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO244_002955"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO244_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5664700.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO244_002956"
     CDS             2703..2939
                     /locus_tag="OO244_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5664701.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO244_002957"
     CDS             2942..3799
                     /locus_tag="OO244_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5664702.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO244_002958"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO244_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO244_002959"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO244_002959"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5664703.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO244_002960"
     CDS             complement(6414..7310)
                     /locus_tag="OO244_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5664704.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO244_002961"
     CDS             complement(7359..7598)
                     /locus_tag="OO244_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5664705.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO244_002962"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO244_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5664706.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO244_002963"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO244_002963"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5664707.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO244_002964"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO244_002964"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5664708.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO244_002965"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO244_002965"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5664709.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO244_002966"
     CDS             10765..12879
                     /locus_tag="OO244_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5664710.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYAIDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DGIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO244_002967"
     CDS             12889..14181
                     /locus_tag="OO244_002967"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664711.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO244_002968"
     CDS             15131..15223
                     /locus_tag="OO244_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5664712.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>16358)
                     /locus_tag="OO244_002969"
     CDS             complement(15267..>16358)
                     /locus_tag="OO244_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5664713.1"
                     /translation="NENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSL
                     KDLERAYKNFFRKRAAFPRFKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSR
                     QVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFE
                     PVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTV
                     SKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQ
                     LWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3728 a         4320 c         4288 g         4022 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aatatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgcca tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccgcaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacggcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatcggg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    15661 ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt cgttgtgact
    15721 ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt
    15781 ttctgcttct gccagttgtt gctgaatttg accttccggc ttaactgtcg ctgaagtctc
    15841 gccagcgtct tctggttttt ttgaaaactg tttacaggct caaagactgt gccatctgac
    15901 agcgtggcga gtttagccac gccagcatcc agcccgatca ttgatgctga agggtgaacc
    15961 ggagttgata cttcactttc tgtctgaata ctgatgtacc acgtaccgca ggactggctg
    16021 acagtgacat ttttcacaac tcccgtgacc tgacggctat tccggtagcg catccagcca
    16081 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgtcctg cgggtagcgg
    16141 aatgcagcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ccggaagaag
    16201 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga gggcgaatct
    16261 ttaagccatt gcgtttcagt ggcgtttttc cactcaacca gccaggaagc cattttcccg
    16321 taagggatgt atttattacc ggcctcatga ttctcatt
//
LOCUS       DAJTOW010000087        15937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTOW010000087 DAJTOW010000000
VERSION     DAJTOW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.087"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            450..1508
                     /gene="citC"
                     /locus_tag="OO244_002970"
     CDS             450..1508
                     /gene="citC"
                     /locus_tag="OO244_002970"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5664714.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1523..1819
                     /gene="citD"
                     /locus_tag="OO244_002971"
     CDS             1523..1819
                     /gene="citD"
                     /locus_tag="OO244_002971"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5664715.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALQARASGIPALPWEDCQ"
     gene            1816..2724
                     /gene="citE"
                     /locus_tag="OO244_002972"
     CDS             1816..2724
                     /gene="citE"
                     /locus_tag="OO244_002972"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5664716.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGAHAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2735..4267
                     /gene="citF"
                     /locus_tag="OO244_002973"
     CDS             2735..4267
                     /gene="citF"
                     /locus_tag="OO244_002973"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5664717.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4271..4822
                     /gene="citX"
                     /locus_tag="OO244_002974"
     CDS             4271..4822
                     /gene="citX"
                     /locus_tag="OO244_002974"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5664718.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4797..5675
                     /gene="citG"
                     /locus_tag="OO244_002975"
     CDS             4797..5675
                     /gene="citG"
                     /locus_tag="OO244_002975"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5664719.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5726..7188
                     /gene="citT"
                     /locus_tag="OO244_002976"
                     /pseudo
     CDS             5726..7188
                     /gene="citT"
                     /locus_tag="OO244_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7302..8108
                     /gene="rna"
                     /locus_tag="OO244_002977"
     CDS             7302..8108
                     /gene="rna"
                     /locus_tag="OO244_002977"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5664720.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8337..8747
                     /gene="rnk"
                     /locus_tag="OO244_002978"
     CDS             8337..8747
                     /gene="rnk"
                     /locus_tag="OO244_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5664721.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8978..10216)
                     /locus_tag="OO244_002979"
     CDS             complement(8978..10216)
                     /locus_tag="OO244_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5664722.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10437..10865
                     /gene="uspG"
                     /locus_tag="OO244_002980"
     CDS             10437..10865
                     /gene="uspG"
                     /locus_tag="OO244_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5664723.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10986..12551)
                     /gene="ahpF"
                     /locus_tag="OO244_002981"
     CDS             complement(10986..12551)
                     /gene="ahpF"
                     /locus_tag="OO244_002981"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5664724.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12796..13359)
                     /gene="ahpC"
                     /locus_tag="OO244_002982"
     CDS             complement(12796..13359)
                     /gene="ahpC"
                     /locus_tag="OO244_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5664725.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13731..14477
                     /gene="dsbG"
                     /locus_tag="OO244_002983"
     CDS             13731..14477
                     /gene="dsbG"
                     /locus_tag="OO244_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5664726.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14687..15589
                     /locus_tag="OO244_002984"
     CDS             14687..15589
                     /locus_tag="OO244_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5664727.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15736..15937
                     /locus_tag="OO244_002985"
                     /pseudo
     CDS             15736..15937
                     /locus_tag="OO244_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3958 a         4120 c         3855 g         4004 t
ORIGIN      
        1 aaaagatacg cagcggaaat gccagtcttt ggaaaaatgc aaactgttta ttctcgttaa
       61 gctgcaacat tgttttcctt gcgggttgtc ccgagcctct ttgacggatg taattaaaat
      121 gttgaatcaa aatatataga tattaattat gatcgtggca aattttattc attaaatcaa
      181 ttaaatcact taaacctgcg agtcatttcg taattacatt aattaaaaaa cttaaaacca
      241 ttaaataaat aaaaaaccac taactctatg tgaaataaat caaaatttca cgccgaaata
      301 ctccttagga tgtatagcga aaggagaaaa agatatacct cgatcacccc cctttctccc
      361 aagtaaaaat aaaaggttat cagtttgcaa cattgaacaa cattcgttgc aaatcgataa
      421 caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc acccgcgtaa
      481 aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa aatgatttga
      541 gcgttgacac cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt
      601 gcggtggaat tgccggaaat attattaaat gcgttgctat cagtgaatcc gttcgcggtg
      661 aaggactggc gctgacatta gccactgaac tgataaacct cgcctatgag cggcacagca
      721 cgcatctgtt tatttatacc aaaaccgaat acgaggcgct gttccgccag tgcggttttt
      781 ccacgctgac ctgcgtaccc ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga
      841 aacgctatgc cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt ggctgcattg
      901 tgatgaacgc caatcccttt acgaatggtc accgttatct gattcaacaa gctgcggcac
      961 agtgcgactg gttgcatctg tttttagtca aagaagattc ttcacgcttc ccctatgaag
     1021 accggctgga tctggtgtta aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg
     1081 gctcagaata catcatctcc cgcgctacgt tcccttgcta cttcattaaa gaacagagcg
     1141 tcattaacca ttgttacacc gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg
     1201 cgcttggtgt aactcaccgc tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt
     1261 acaaccagga tatgcgctac tggctggaaa cgccgactat ctccgcagcg cccatcgaac
     1321 tggttgaaat tgagcggctg cgttaccagg agatgccgat atccgcttcc cgggtacgtc
     1381 aactgctggc gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc
     1441 attatttgca gaacctgctt gagcactccc gccaggacgc ggcagctcgt caaaagaccc
     1501 ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag gcacccttga
     1561 gtctggggat gtgatgatac gcatcgcccc actcgatacg caggatatcg acctgcaaat
     1621 caatagcagc gttgagaaac agtttggcga tgcaattcgc accaccattc tggacgttct
     1681 cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat gacaaaggcg cactggactg
     1741 cattttacat gcacgactgg aagccctgca ggcacgcgcc agcggtatcc cggctctgcc
     1801 atgggaggat tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc
     1861 agcatgttgt ttgtgcctgg tgcccatgcc gcgatggtca gcaactcctt catctacccg
     1921 gctgatgccc tgatgtttga cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc
     1981 cgccgcatgg tttatcacgc gctgcaacat ccgctgtatc gcgatattga aaccattgtg
     2041 cgtgtcaacg cgctggattc cgaatggggt gttaacgacc tggaagccgt cgttcgcggt
     2101 ggtgcggacg ttgtgcgtct gccgaaaacc gataccgctc aggatgttct ggatattgaa
     2161 aaagagatcc tgcgtataga aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg
     2221 gcggcgattg aatctccgct gggcattacc cgcgcagtgg aaatcgctca cgcttccgag
     2281 cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc
     2341 tccccggaag gaactgaact gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct
     2401 gcgggtattc aggcgttcga taccgtctat tccgacgcta acaacgaagc cggattcctg
     2461 caagaagccg cccacatcaa acagctgggc tttgacggca aatcgctgat caacccgcgt
     2521 cagattgatc tgctgcacaa cctctacgca ccgacccaga aagaagtgga tcacgcccac
     2581 cgcgtcgtag aagccgctga agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac
     2641 ggcaagatgg tggacggtcc ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa
     2701 ctttccggca tccgcgaaga ataaggcaat caaaatgacg cagaaaattg aacaatctca
     2761 acgacaagaa cgggtagcgg cctggaatcg tcgcactgaa tgcgatcttg ccgctttcca
     2821 gaactcacca aagcaaacct accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa
     2881 cctggaagaa gcgattcgtc gctcaggttt acaggacggc atgacggttt ccttccatca
     2941 cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg
     3001 ctttaaaaac ctgaccctgg cgtccagctc cctgagtgat tgccatgcgc cgctggtaga
     3061 acacattcgc cagggcgtgg ttacccgcat ttatacctct ggcctacgcg gtccactggc
     3121 ggaagagatc tcccgtggtc tgctggcgga accggtgcag atccactctc acggcggtcg
     3181 tgtgcatctg gtacagagcg gcgaactgaa tatcgacgtg gctttcctcg gcgtcccgtc
     3241 ctgtgatgaa ttcggtaatg ccaacggcta caccggtaaa gcctgctgcg gctccctcgg
     3301 ctatgcaatg gttgatgccg acaacgcaaa acaggtcgtg atgcttaccg aagaactgct
     3361 gccttatccg cataatccgg caagcattga gcaagatcag gttgatttga tcgtcaaagt
     3421 taatcgcgtt ggcgatgctg caaaaatcgg cgctggtact acccgtatga ccactaaccc
     3481 gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa
     3541 agaaggtttc tccatgcaaa ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct
     3601 ggaagacaaa atgcgtagcc gcgatattcg cgccgacttc gccctcggcg gtattaccgc
     3661 gacgatggtt gatctgcacg aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt
     3721 tgacagccat gctgcgcaat cgctggcccg taaccccaat cacatcgaaa tcagcgccaa
     3781 ccagtacgct aactggggtt cgaaaggcgc atcggttgat cgtctcgacg tggtggtact
     3841 gagcgcgctg gaaattgaca ggcagttcaa cgttaacgtg ctgaccggtt ctgacggcgt
     3901 actgcgtggc gcttccggtg gtcactgcga taccgcgatt gcctctgcgc tttccatcat
     3961 cgtcgcgcca ctggtacgcg gtcgtattcc gactctggtg gataacgtgc tgacctgcat
     4021 caccccgggc tccagtgtcg atattctggt cacagaccac ggtatcgcag ttaacccggc
     4081 acgtccggaa ctagcagaac gtctgcagga agcgggcatt aaagtggttt ccattgagtg
     4141 gctgcgcgaa cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg
     4201 cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa
     4261 ggaataagcc atgcacctgc ttcctgaact cgccagccac catgcggtgt caattcccga
     4321 gctgctcgtc agccgggatg aaaggcaagc acggcaacac gcctggctca agcaccatcc
     4381 tgttccactg gtctccttta ccgtggttgc gcctgggccg attaaagaca gcgaggtcac
     4441 acgccgaatt tttaatcatc gcgtgacagc cttgcgtgcc ttagccacaa agcagggctg
     4501 gcaaattcag gagcaggctg cactggtttc cgccagcggg ccggagggca tgttgagcat
     4561 tgccgccccg gctcgcgacc tcaagctcgc caccattgag cttgaacata gtcatcctct
     4621 cgggcggtta tgggatatcg atgtcctgac gcccgaaggc gaaattctct cccgccgcga
     4681 ctattcactg ccgcctcgcc gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg
     4741 tggaaaaacc catcaactga ccgatttact caaccgcatg gaggcactgc tgaacgatgt
     4801 cgatgcctgc aacgtcaact aaaaccacaa agcttgcgac gtcattaatc gatgagtacg
     4861 ccctgctggg ctggcgcgcc atgctgactg aagtcaatct gtcaccgaaa ccaggcctcg
     4921 tggatcgcat taactgcggt gcgcacaaag atatggcgct ggaagatttc caccgcagcg
     4981 cgctggcgat tcagggctgg ttaccccgtt tcattgaatt tggtgcctgt agtgcagaaa
     5041 tggcaccgga agcggtactc cacggattac gcccaattgg tatggcttgc gaaggtgata
     5101 tgttccgcgc cactgcgggc gtaaacacgc ataaaggcag cattttttct ttagggctgc
     5161 tatgtgcggc aattggccgt ttgcttcagc tcaaccagcc ggttacgcca acaaccgttt
     5221 gttctacggc ggcaagtttc tgccgtggcc tgaccgatcg cgaactgcgt accaataatt
     5281 cacgactgac ggcaggtcaa cggttgtacc agcagcttgg ccttaccggc gcacgcggtg
     5341 aagccgaagc aggttatcca ctggtgatca accacgcctt gccgcattac ctcactctgc
     5401 tggatcaggg gttagatcct gaactggcat tgctcgatac cttgctccta ctgatggcta
     5461 ccaacggcga taccaacgtt gcatcgcgcg gtggcgaggg gggcctgcgc tggctacagc
     5521 gcgaggcgca aacattattg caaaaagggg gcattcgaac ccccaccgat ctcgattatc
     5581 tccggcagtt cgacagggag tgtatcgaac gaaatctcag tccaggcggc agtgccgacc
     5641 tgctgatcct tacctgtttt ttagcacaga tttaattatt taagcacttg ataaatttgg
     5701 aaatattaat tttcggagaa cccgtatgtc tttagcaaaa gataatatat ggaaactatt
     5761 ggccccactg gtggtgatgg gtgtcatgtt tcttatccct gtccccgacg gtatgccgcc
     5821 gcaggcatgg cattacttcg ctgtgtttgt ggcaatgatt gtcggcatga tcctcgagcc
     5881 aattccggca acagcgatca gttttattgc ggttactatt tgcgttattg gcagtaatta
     5941 cctgctcttt gatgccaaag aattagctga cccagcgttt aatgcgcaaa aacaggcgct
     6001 gaaatggggc ctggctggtt tttccagcac cacggtatgg ctggtatttg gcgcatttat
     6061 ttttgcatta gggtatgaag tttccgggtt aggtcgtcgc attgcccttt tcctggtgaa
     6121 attcatggac aaacgcacgc tgacgttggg ttatgcgatt gtcattatcg acattctgct
     6181 ggcaccgttt acaccgtcca acaccgcgcg taccgggggt acggtttttc cggtcattaa
     6241 aaacctgccg ccgttgttta aatcattccc gaacgatccg tccgcgcgtc gtattggcgg
     6301 ctatttgatg tggatgatgg tcattagtac cagtctgagt tcgtccatgt ttgtcaccgg
     6361 tgcggaccaa acgtgctggg tctggagttc gtcagcaaaa ttgccggtat ccagattagc
     6421 tggttgcagt ggttcctctg cttcctgccg gttggggtta tcttgcttat cattgcgccg
     6481 tggctttcct acgtgctgta caaaccggaa atcacacaca gtgaagaagt ggcaacctgg
     6541 gcgggtgatg aactaaaaac catgggtgcg ctgacacgca gagagtggac gctgattggc
     6601 cttgtattgc tcagcttagg tttgtgggta tttggcagtg aagtcattaa tgctactgcg
     6661 gttggtctgc tggcagtttc gctaatgctg gctctgcacg ttgtgccgtg gaaagacatt
     6721 acccgctata acagcgcatg gaacacgctg gtcaacctgg caactctggt tgtgatggct
     6781 aacggcctga ctcgttctgg ttttattgac tggttcgccg gtaccatgag tacgcacctg
     6841 gaaggattct caccaaacgc aacggtgatt gtactggttc tggtgttcta ctttgcacac
     6901 tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc tgccggttat tctggccgtc
     6961 ggtaaaggta ttccgggcgt accaatggaa caactgtgta tcctgctggt gctgtctatc
     7021 ggtatcatgg gctgtctgac gccgtatgca accggtcctg gggtgattat ttacggctgt
     7081 ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa tcttcggggt gatttacatc
     7141 tctatgttgc tgttggttgg ctggccgatt ctcgccatgt ggaactaata cgttcgccaa
     7201 acgctgaata aatcatcacg cccgccaggt aagcccacct ggcgggcttt tttatgattt
     7261 aatagatagt attcagtgta tgtatgagtt ccacacccat tatgaaagca ttctgccgta
     7321 acgccgcgtt gctcgcggtt tctctgcttc ccttatcttc tgccaacgcc gtagcgttgc
     7381 aggcaaaaca gtatggcgat tttgatcgct atgtcctggc cctctcctgg caaaccggat
     7441 tttgccagag tcaacacgat cgaaatcgta acgaacgaga tgaatgtcgc ctgcaaaccg
     7501 aaacgaccaa caaaactgat tttctgaccg tacatggtct gtggccagga ttgcctaaat
     7561 cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg cttcggttgc gctactcgcc
     7621 caatcccgaa tctaccagaa gcacgcgcca gccgaatgtg ttcatcgccg gaaaccgggt
     7681 tatcactgga aacggccgct aaactaagtg aagtcatgcc aggagctggc ggacgttcct
     7741 gcctggaacg ctacgaatat gccaaacacg gtgcctgctt tggttttgat ccggacgcat
     7801 acttcggtac gatggtacgc ctgaatcaag aaattaaaga gagcgaagcc ggaaaattcc
     7861 ttgcggataa ttacggtaaa acagtgagcc gccgtgactt tgacgccgcc tttgccaaaa
     7921 gctggggaaa agagaacgtg aaagcagtta agctaacgtg ccagggtaac cctgcgtatt
     7981 tgactgaaat tcagatctcg atcaaagctg acgccatcaa cgctccgctt tctgcaaact
     8041 cattcttgcc acaacctcac ccaggtaact gtggcaaaac ctttgtgatt gataaagcgg
     8101 gttattaatc ctctgattta caaaacacgc taattcgtct tccataacca tgccgattaa
     8161 gaattataca taatgcgctg cattgaggaa ttccctgaat gtgacgcaaa tcacttcaaa
     8221 gagtgagcaa cagtcagtat catcatgtga gttaaaccct cgccgcctga cggtgagggt
     8281 tttcttttgg gtatgtttat ctgcgacact cgcagtaccg acgacatgga gtaaaaatgt
     8341 ccagaccaac tatcatcatt aacgacctgg atgccgaacg catcgatatt ctgctggagc
     8401 aacccgccta tgctggtttg ccaatcgccg acgcgttaaa cgcagagttg gatcgcgccc
     8461 aaatgtgttc gccagaagag atgccacacg acgtggtgac aatgaacagc cgggttaaat
     8521 tccgcaatct tagcgatggc gaagtgcgtg tgcgcacgct ggtgtatccg gcaaaaatga
     8581 ccgatagcaa tactcagctt tccgttatgg ctccggtagg tgccgcactg ctggggctgc
     8641 gcgttggcga ttccattcac tgggaacttc cgggcggcgt tgcaacccac cttgaagtgc
     8701 tggaactcga ataccagcca gaagctgctg gcgactacct gctttaatcc catctgcaca
     8761 agcgataact cgctttgccc agaggatgca ctcgcatcct ctttacgcaa aatcagcaat
     8821 ttttcctggt tctcactgtt cacttttgct gcaaaattat tccgtttcgt ttgtgggaat
     8881 aaccatggca gaagcatttt acatcctgat aggatttctg attatggcgg cgattatcgt
     8941 catggctgtc ctttacctcg aaaaccactc ctgacgatca tattgttccc cccggcatcg
     9001 cattcaccag acctgaaacc gccttctgcg ccgcctctgc gctttgtgca ccgggtacca
     9061 gaatcacctt acggcactcc tcttcacgtt tttcgaaaat ctcatatccc cgggcggcct
     9121 cttcaaacgg catatagtgg gtaacaattt cttctggttt cagtaatcct ttctcaatta
     9181 acggtaataa ttctcccagc catgcgtgaa cgtgggtctg tcccatttta aacgtcaacc
     9241 ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa tccagcgtag acgcccggta
     9301 cgctaacaat gccgccacgc ctgaccgccg caatacactg gcgcaacgct ttaccgctgc
     9361 tgccctccag tttcaggtta gtcagcaccg tttccgtggt gctgcctttc gcttcaaaac
     9421 cgacggcgtc tattactgca tccacgcccc ggtgacctgc cgtttgttca ataattgact
     9481 gtgccggatc gctatcttca tcgaaattaa tcgggatcgc gccgtagcgg tcagcggcga
     9541 aacgcaagcg gtagggatga tgatcaacaa caaaaatctg ttccgcaccg agcaaccgtg
     9601 cacaggcgat tgtcaacaat cccacaggac cagcaccata gactgcaacg cttgaacctt
     9661 gttggatctg cacatttttt gctgcctgcc atgccgttgg cagaatatca gaaaggaaaa
     9721 gggctttatc atctgaaagc aaaggcggta ctttaaacgg ccccacattc cctttcggga
     9781 cgcggacata ttccgcctgc ccgccaggaa cgccaccata caggtgacta taaccaaaca
     9841 atgccgcggg agcaggtatc tgttttttat tgagcgcagc gcctttaccc gcattggtat
     9901 tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca gtcgccacaa gcaatgacga
     9961 acggaattac cacccggtcg cctttttgca aattttttac gtccttcccg gtttcaacga
    10021 cttcccccat aaattcatga ccaaaaatat cgccatgttt aacctggggt attttgcctc
    10081 gataaagatg gaggtcagag ccacagatcg ccgttaccgt aatacgcaga ataatatcat
    10141 ctgcctgttc aatgcccgga tcgggaacat tttctacctg aacgtgatgt gggccgtgat
    10201 aagtcaatgc tttcatgcaa cctccagaga cagttcaaaa aagagtcagt ctttaagggt
    10261 agctatcaca gcgggaaaac gggcggtacc gcaggttttg ggagaattcc tagtgttcag
    10321 tgagaatata ttaatcacag aaaccgttcg taaggaaaaa gaaccagaca aatatcaggg
    10381 ataaatatta taatcatgga gaatgatttg ttcatgatta acagggagaa agggttatgt
    10441 ataaggcaat cattatgcca gttgatgtat ttgaaatgga attgagcgac aaagctgttc
    10501 gccacgctga attcctcgcc caggatgacg gagttattca tctacttcac gtactacccg
    10561 ggtcagccag cctgagcctg caccgttttg ccgctgatgt gcgtcgtttt gaagagcatc
    10621 tgcaacatga agcagaagaa cgtctgcaaa cgatggtcag ccacttcacc atcgatcctt
    10681 cccgcattaa acaacatgtc cgttttggta gcgtgcggga tgaagtcaat gagttggcag
    10741 aagaactggg ggctgatgtt gtagttatcg gttctcgcaa cccatcgatt tcgacccatc
    10801 tgttaggttc taacgcctcg agcgtgatcc gccacgccaa tctgcctgta ctggtcgttc
    10861 gctaacagat aaaagtattt atgccgccct gcctggaaca tcccggcagg gctttttaat
    10921 gccataaaaa agccgccagg tttgaccccg gcggctaagc aattgcaggt gaatcttact
    10981 tcttcttatg cagttttggt gcgaatcagg tagtcaaaag cactcagaga ggctttggca
    11041 ccttcgccag tggcgatgat gatctgcttg tacggaaccg tcgtacagtc acccgctgcg
    11101 aacacgcctt tgacgttggt ttcacatttc gcatcaatga taatctcgcc catgcggtta
    11161 cgttcgactg cgccttccag ccagttggtg ttcggcagca gaccaatctg gacaaaaata
    11221 ccggccagtt cgatgttgtg aatattgccg ctgacacgat ctcgatattc cagaccaacg
    11281 actttgctgc cgtcgccttt cacttccgtg gtttgcgcat tcagaataat gtcgacgttt
    11341 ttcaggctgc gcagtttgtc ctgcagaacc tggtcggctt tcatttctgg cgcaaattcc
    11401 agcagcgtta cgtgctcaac gatacccgcc aggtcgattg ccgcttccac gccggagtta
    11461 ccgccgccga taaccgctac gcgtttgcct ttaaacagcg ggccgtcgca gtgcgggcag
    11521 taggtcacgc ctttagtgcg atactgatct tcgcccggaa cgttcatgtt gcgccatttt
    11581 gcacccgtcg ccacgataat gctgcgtgct ttcagtaccg cgccagaagc tgtttcaatc
    11641 tggtgtagac caccttcaac cgctgccggg atcagtttgc tggcgctctg gctgtcgatc
    11701 acatcaacgt cgtattcatc aacgtgaact ttcagtgcgc ctgccaactt ctgcccttca
    11761 gtcttcggta cagaaatgta gttttcgata tcaacggtat cgaggatctg accaccaaaa
    11821 cgttcgccca tcagaccggt acggatgcct ttacgtgcgg agtaaattgc tgccgctgca
    11881 cccgccgggc cggaaccaac gattaatacg tcataagcat cacgcttgtt cagctcttct
    11941 gccgcacgat tttccgcgcc agtatcaatt ttggcaacga tttcagtcaa cgtcatgcgg
    12001 ccctgaccaa actctttccc gtttacgaac actgccggaa cgcccatcac gttgcgatcg
    12061 gtgatttcgt tctggaaggt gccgccgtca attgcagtgt gcttgatgcg cgggttcaat
    12121 acgctcatca ggttcagcgc ctgcaccacg tccgggcagt tatggcaaga gagcgagtaa
    12181 taggtttcga attcaaaatc accgtcaata tggcgaatct gctccagcag agactgcgct
    12241 tctttcgacg gatgaccacc ggtccacagc aacgccagta ccagcgaggt gaactcgtgg
    12301 cccagcgggg agcctgcaaa acgtggtccc tggttggaac ctgggttggt gatcaggaaa
    12361 gacggcttac gcactggcaa gctgttatct tctttaaagg tgactttgtc tgacagttct
    12421 gcgatttcag ccaacagttc cttgatttct gccgatttag cgctgtcatc cagcgtggca
    12481 attaactcaa caggcttggt caatttctca aggtaagcct tgagttgagt tttcatattt
    12541 gtgtcgagca tgtttatctc ctgggcttta aacatcatca tgcaagcggc ctctgcggct
    12601 acctggatgc agcttgcatc atggtgcggg gaagaaaatc gggtgccgca gcacccgaag
    12661 aattaggtga atttccgaag tatttcgcgt cagagcaagg cgactagcct gtgaatcccc
    12721 gggagcttac acaagtaagt gaccggggtg agcgggcgac gccaacgccg ctatggcgtg
    12781 aaagacgaag gaaatttaga ttttaccaac caggtccaga gacggagcca gagttgcttc
    12841 accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt actgagctgc
    12901 tttgatttta cgcagcagat cagacgcgtc acggccaatg ccttcagcgg taacttcgat
    12961 tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc
    13021 ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata
    13081 tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc
    13141 ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac
    13201 gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct
    13261 ccagcggcct tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg
    13321 gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc
    13381 gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc
    13441 gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc
    13501 gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta
    13561 tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa cgacatggat
    13621 tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga
    13681 taggttatgc cttttactcg acttttgcac tgactgaaaa gaacaaatta atgttaaaaa
    13741 agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt ccttccccag
    13801 taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac gttcgatgcc cccggaggaa
    13861 tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg actcccgatg
    13921 gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg agtaacacac
    13981 ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg atggaacaat
    14041 cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtc ttctccgatc
    14101 cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta gattctggca
    14161 aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag
    14221 cggcaattct tgcctccaaa gatcccgcaa aaacctggca acaatatgaa gcctctggtt
    14281 gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga gcaaatgaaa gtgttaagtg
    14341 acaatgagaa actgatggac gatctggggg caaatgtcac gccggctatc tattacatga
    14401 gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg cttaatatca
    14461 ttatgggaaa taaataaaag gtaattcatg gatggttgaa ctatatccct gcttttcaac
    14521 catcctaaaa aagcatgaat ttctcgctct accctaagtg gctgtgctat tttagaaaaa
    14581 cttaatcctg agttaagcaa aaaaacataa tcaataaaaa tacatacgca aaaaacattg
    14641 attaagtgaa tataccatgg aagaaaaata taaaccggag tagtgtatgg ccaatctcta
    14701 cgacttgaaa aagttcgact taaatcttct tgtcatattt gagtgtattt accagcattt
    14761 aagcattagc aaagctgctg aatcactata tatcacccca tcagccgtga gccagtcgtt
    14821 acaacgctta cgtgcgcaat ttaatgaccc tttattcatt cgctcaggaa aaggtatcgc
    14881 gccgacgaca acagggttga atctgcatca ccatcttgag aaaaacctca aaggccttga
    14941 acaaacaatt aatatagtca ataagtccga actaaagaaa aactttatta tctacggccc
    15001 tcagctcatt tcttgctcta ataatagtat gctgatccgc tgcctacggc aagatgcctc
    15061 tgtcgagatt gagtgccatg atatcctcat gtcagcagag aatgctgaag aactgttggt
    15121 tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg atcagccgtt ccgtcatttg
    15181 catgccattg catacaattc gcaatactct aatttgtagt aataagcatc cgcgaattac
    15241 tgacaatagc acttatgaac aaattatggc agaagagttc acccaattaa tagccaaatc
    15301 agcaggagtt gatgatattc aaatggaaat agacgagaag tttatgaatc gcaaaatttc
    15361 atttcgtggt tcctctttgc tgacaataat taatagcatt gcagttacag atttactggg
    15421 aatagtcccc tatgaacttt ataactacca ccgtgacttt cttaatttaa aagagataaa
    15481 actagaacat ccgttacctt caataaagct ttacatttcc tataataaat cttctttaaa
    15541 taatttggtg ttctcccgat ttattgaccg tctaaatgag agtttttgat atataactat
    15601 agacagctac tataactttc atctatttat tcacagcgcg aatgaaaaat aaaacactct
    15661 gctttacagt gtttacttta taattaacag tcttatctca ctgttcaccc tgtaacgttc
    15721 agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat tctggaagct
    15781 gcacgagagc gtattaccta gacactcaac accctacccc gcgtttgtgt ctcattttct
    15841 ggcggcaaag actctggttt aatgctgcat ctgacagccg aacttgcccg acaaatgggc
    15901 aaaaaaatct gcgttttgtt tatcaactgg gaggcgc
//
LOCUS       DAJTOW010000088        15527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTOW010000088 DAJTOW010000000
VERSION     DAJTOW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.088"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO244_002986"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO244_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5664728.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO244_002987"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO244_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5664729.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO244_002988"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO244_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO244_002989"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO244_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5664730.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO244_002990"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO244_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5664731.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO244_002991"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO244_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5664732.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO244_002992"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO244_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5664733.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO244_002993"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO244_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5664734.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO244_002994"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO244_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5664735.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO244_002995"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO244_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5664736.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO244_002996"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO244_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5664737.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO244_002997"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO244_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5664738.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO244_002998"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO244_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5664739.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO244_002999"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO244_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5664740.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO244_003000"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO244_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5664741.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO244_003001"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO244_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5664742.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO244_003002"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO244_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5664743.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO244_003003"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO244_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5664744.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO244_003004"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO244_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5664745.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO244_003005"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO244_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5664746.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO244_003006"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO244_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5664747.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO244_003007"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO244_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5664748.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO244_003008"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO244_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5664749.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO244_003009"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO244_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5664750.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO244_003010"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO244_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5664751.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO244_003011"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO244_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5664752.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO244_003012"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO244_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5664753.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO244_003013"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO244_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5664754.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO244_003014"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO244_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5664755.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGMLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO244_003015"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO244_003015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5664756.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO244_003016"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO244_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5664757.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4008 a         3644 c         4129 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggta tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tcttttt
//
LOCUS       DAJTOW010000089        15327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTOW010000089 DAJTOW010000000
VERSION     DAJTOW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.089"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..1111)
                     /locus_tag="OO244_003017"
     CDS             complement(<1..1111)
                     /locus_tag="OO244_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5664758.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQ"
     gene            complement(1310..1394)
                     /locus_tag="OO244_003018"
     tRNA            complement(1310..1394)
                     /locus_tag="OO244_003018"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1358..1360),aa:Leu,seq:caa)
     gene            1590..2609
                     /gene="ahr"
                     /locus_tag="OO244_003019"
     CDS             1590..2609
                     /gene="ahr"
                     /locus_tag="OO244_003019"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5664759.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2738..4240
                     /locus_tag="OO244_003020"
     CDS             2738..4240
                     /locus_tag="OO244_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5664760.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4359..5441)
                     /gene="lptG"
                     /locus_tag="OO244_003021"
     CDS             complement(4359..5441)
                     /gene="lptG"
                     /locus_tag="OO244_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5664761.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5441..6541)
                     /gene="lptF"
                     /locus_tag="OO244_003022"
     CDS             complement(5441..6541)
                     /gene="lptF"
                     /locus_tag="OO244_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5664762.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6808..8319
                     /gene="pepA"
                     /locus_tag="OO244_003023"
     CDS             6808..8319
                     /gene="pepA"
                     /locus_tag="OO244_003023"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5664763.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8453..8896
                     /gene="holC"
                     /locus_tag="OO244_003024"
     CDS             8453..8896
                     /gene="holC"
                     /locus_tag="OO244_003024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5664764.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8896..11751
                     /gene="valS"
                     /locus_tag="OO244_003025"
     CDS             8896..11751
                     /gene="valS"
                     /locus_tag="OO244_003025"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5664765.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11805..13001)
                     /locus_tag="OO244_003026"
     CDS             complement(11805..13001)
                     /locus_tag="OO244_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5664766.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13194..13697
                     /locus_tag="OO244_003027"
     CDS             13194..13697
                     /locus_tag="OO244_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5664767.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13743..14159)
                     /gene="rraB"
                     /locus_tag="OO244_003028"
     CDS             complement(13743..14159)
                     /gene="rraB"
                     /locus_tag="OO244_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5664768.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14321..>15327
                     /gene="argF"
                     /locus_tag="OO244_003029"
     CDS             14321..>15327
                     /gene="argF"
                     /locus_tag="OO244_003029"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5664769.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     L"
BASE COUNT         3951 a         3911 c         3955 g         3510 t
ORIGIN      
        1 cctggtgact catctgccgc tctaccgcat ccctcgacca taatcccgac tcaatcaacg
       61 aactacaagc catcgtcctg aagccatgcc cacaaacctc gaccttcgtg tcatacccca
      121 ttacgcgcaa agccttgttc accgtattct cactcatcgg cttacgtgga tcgtgatcgc
      181 ccacaaaaat cagctctcga tttccgctca tgcttttgat cttttccaaa atacttaggg
      241 cctgccgcga caaaggtaca aggtgtggtg tccgcatctt cgagccacgc tgagaatgct
      301 tgactccttc cagttgctca cgctcacctg ggattgtcca catagccgtt tcaaaatcta
      361 tttctgacca gcgggcaaaa cgcagctcgc tcgaacggat gaaaaccaat aaagtgagtt
      421 caaccgccag tcgagttaac ggtctgccag aatagtgatc tatgcgatga agtaattcag
      481 gtatacggtt cagttcaaga gctgcacggt gctgtctttt cgccgtagca accgcacctg
      541 caatctcttg cgcggggttg tagtcgatta agccgctctg aacggcaaag cgcataatcg
      601 cggtagtacg ctgctgtaaa cgggcggcaa cttcgagccg cccggatgac tcgactgctt
      661 taatgggtac aagaagatcc cgcgtcttca gttccgcaat gttccgctta ccgatagcag
      721 taaagagatt atcctccaga cttttcaaaa cacgagcgct atgcgatgcc gaccacttct
      781 gattgctggc gtgccagtct ctggcgacta cttcaaacgt tatcgcctct tgttcctgtt
      841 ctaccttaac agctttcttg ttttcactgg gatcgacacc attagctaaa agcttacgag
      901 cctcatcccg gcgtgccctg gcatccgcca acgacacttc ggggtatttc cccaatgcca
      961 gcatcttctc tttgccgcca aagcgataac gtagccgcca gtatttggag ccgttagggt
     1021 gaaccagcaa aaccatgcct tcaccgtcgg tcagcttata ggcttttgct tcaggcttag
     1081 ccgaacgaac cttcacatca ctcagagcca tgatgagtat cctttcaagg gttctgtgtg
     1141 ggtacaaaca ttatcgaacc gggaaatacc cgcagttgta cccgcatcag taagttgatg
     1201 tagattgaat caggttgact taggttgagt gaaaaagcga ggaaagcctt gcggatactg
     1261 gattttaggc acaaaaaaag acgtccgttg acgtctattg atgttccgat ggtgccgaag
     1321 gccggactcg aaccggcacg tatttctacg gttgattttg aatcaactgc gtctaccgat
     1381 ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg
     1441 ccaccatgca agcgccagct tgcacccacc tcgctaagtg ctgaaatttt cagcattaca
     1501 ctcagcgata tgtaaccttt gtcacactcc aggcaccccg ccctgccatg ctctacactt
     1561 cccaaacaac accagagaag gccccaaaaa tgtcgatgat aaaaagctat gccgcaaaag
     1621 aagcgggcgg cgaactggaa gtttatgagt acgatcccgg tgagctgaag ccacaagatg
     1681 ttgaagtgca ggtggattac tgcgggattt gtcattccga tctgtcgatg atcgataacg
     1741 aatggggatt ttcacaatat ccgctggttg ccgggcatga ggtgattggg cgcgtagtgg
     1801 cactcgggag cgccgcgcag gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct
     1861 ggacggcgcg tagctgtggt cactgcgacg cctgtattag cggtaatcag atcaactgcg
     1921 agcaaggtgc ggtgccgaca attatgaatc gcggtggctt tgccgagaag ttgcgtgcgg
     1981 actggcaatg ggtgattcca ctgccagaaa atattgatat cgagtccgcc gggccgctgt
     2041 tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca ccatatcact gctaccagcc
     2101 gcgttggggt aattggtatt ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa
     2161 tgggatgcga ggtgacggcc tttagttcta atccggcgaa agagcaggaa gtactggcga
     2221 tgggtgccga taaagtggtg aatagccgcg atccgcaggc actgaaagcc ctgtcggggc
     2281 agtttgatct cattatcaat actgtgaacg tcagcctcga ctggcagcct tattttgagg
     2341 cgctgaccta tggcggtaat ttccatacgg tcggtgcggt tctcacgccg ctgtctgttc
     2401 cggcctttac gttaattgcg ggcgaccgca gcatctctgg ttctgctacc ggcacgcctt
     2461 atgagctgcg aaagctgatg cgctttgccg cccgcagcaa ggttgcgccg acaaccgaac
     2521 tgttcccgat gtcgaaaatt aacgacgcca ttcagcatgt acgcgatggt aaagcgcgct
     2581 accgagtagt cctgaaagcc gacttctgat ccctttgccg ggtgctgtcg cccggcactt
     2641 aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag tgcacagccg
     2701 ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc tgttaattgc
     2761 ccgcacgccg gacacagaac tgtttttact gccgggaatg gctaaccgtc acgggctgat
     2821 tactggcgca acggggacgg gtaaaaccgt tacgctgcaa aaactggcag agtcattgtc
     2881 ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg gtatcgcgca
     2941 ggcaggaacg gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg tcaatgactg
     3001 gcaaccgcat gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccacacggt
     3061 gcgggcgacg gtttcggatc tggggccgct gttgctggcg cgactgttga atctcaacga
     3121 tgtgcaatct ggcgtgctga atattatctt ccgcattgcg gacgatcagg gattgttgct
     3181 gctcgacttt aaagatttgc gggcgattac ccagtacatc ggcgataacg ccaaatcctt
     3241 ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc gcggattact
     3301 gtcgctggaa cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga
     3361 ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg ccgagaaact
     3421 ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag agttgtatga
     3481 acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaggc
     3541 acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag
     3601 gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc
     3661 ggataatgtg ttcggccagc tcggtaatcg tgttcagcac gctttgcggg cttttacgcc
     3721 aaaagatcag aaagcagtga agacagcggc gcaaaccatg cgggccaatc cggcatttga
     3781 taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt ttctcgatgc
     3841 aaaaggaagt ccttctgtgg tggaacgggc gatggtgatc gcaccttgtt cgcgaatggg
     3901 gccggtgacg gaagatgagc gtaatggcct gattaatcac tccccggtgt atggcaaata
     3961 tgaagatgag gtggaccgcg agtccgccta tgagatgctg caaaaaggct ttcaggccag
     4021 taccgagcag caaaataatc cccccgtgaa agggaaagag gtggcggtgg atgacggtat
     4081 tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg ggaagaaaga
     4141 tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc agattgtacg
     4201 cgggatgttg gggagtttgc tgggggggag aaaaagataa gtcagacgtg atctgtgccc
     4261 ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt cgaactcgta tttgactttg
     4321 tcaacaataa taaagggagc tttcgctccc ttgttagatt acgattttct cattaacagc
     4381 cacaggctga ttaagaagaa gctggcactt ggcaacagtg cgccgatgat cggcgggatg
     4441 ccgtaaacca acgtcagcgg gccgaagatc tggtccagta cgtagaagac aaaaccgaag
     4501 ctgatacctg tgaccacacg cacgcccatc ggtacgctac gcagtgggcc aaagataaac
     4561 gacagcgcca tcagcatcat caccgccacg gatagcggct ggaagatttt gctccacatg
     4621 ttgagctgat aacgtccggc atcctgaccg ctcgacttca gatacttcac gtagttgtgc
     4681 aaaccactga tggagagtgc atccgggtcc agcgccacca cgcccagttt gtctggcgtg
     4741 aggtcggttt tccaggtgcc gctcaccgtc tgcgaaccgg taatctgttt cggattggtc
     4801 agatcagatt catcaacctg cgacagacgc cagactttat gttccgggtc aaacttcgcg
     4861 gtagcggcat aacgtacgga ttgcagacga cgattctcgt taaaggcata aatactgatg
     4921 ccacctaaca cctcgtcacc tttaacccgc tcaatgtaga cgaagttgtt gccatctttc
     4981 gcccataaac cttgctgggt agagagcaac gagccgccgt acatcgcctg cgcacggtag
     5041 ttacgcgcca tctgttcgcc ctgcggcgcg acccattcgc caatcgccat cgtcagcaag
     5101 accagtggaa tggcggtttt catcaccgac agcgctacct gcatacgggt aaaaccagaa
     5161 gcctgcatca ccaccagttc gctgcgctgc gccagcatcc caagaccaag caacgcccca
     5221 agaagagccg ccatcgggaa gaagatctgc acatctttcg gcacgctcag caaggtatac
     5281 attcctgcgc ctaacgcgtc gtaactcccc tgcccggctt ttttcagctg atcgacaaat
     5341 ttgataatgc ccgacagcga caccagcatg aacagcgtca tcatgatggt ggtgaaaata
     5401 gttttaccga tatagcggtc aagtacgcca aaaggttgca tcacaccgct cctttacgcg
     5461 aaaaactggc gcgcaggcgg cggaccggca cggtgtccca aaggttgaga acaatcgcca
     5521 aagccagata aatcaggtta acggtccaca tccacagtgt cgggtccagc ttacctttac
     5581 cgccgttcga tttcagggag gtctggatca ggaagaaaag tagatacagc agcatggctg
     5641 gcagcatcga cagtacgcgt ccctgacgcg ggttaaccac gctcaacggt acaaccataa
     5701 gtgccatcat aaacacggtg aataccaacg tgatacgcca gttcagttct gcgcgagcac
     5761 gatcggtgtc agtgttccac aatgtgcgca tgtccatctg gtcggtatcg ttcgggtcga
     5821 gcgccaccgc ctggtgacca atgatcgcct gataatcctg gaaatccgta atgcggaaat
     5881 cacgtaacag tgcagtgcct tcgaagcgcg ttccctggtt gagagtgacg acctgggagc
     5941 cgtcgcgcag ctgggttaaa tgtccggaat cggccaccac cacagaagga cgtgcattac
     6001 cttttggtcg aatttgtgcg aggaacacat ctttgaaatc gctgccgtca acgctttcga
     6061 tgaacagcac cgagctgcca ttagtcgctt gctggaattg cccttgcgcc agcgccgcca
     6121 tgccagggtt cgctttcgct tctgctaaca cttcatcctg atgacgcgat gaccacggtc
     6181 ccgcccacat cacgttaacc gccgcgacga ttgccgtgaa taccgcaagg atcattgccg
     6241 ctttcaccag aaccgctttg ctcaagccgc aggcatgcat taccgtaatt tcactttcgg
     6301 tatacagttt gcccagcgtc atcagcagcc cgaggaacag gcttaatggc aggataagct
     6361 gcgccatttc cggcacgccc aacccgagaa gggagagcac cagattcgcc ggaatatcgc
     6421 cgtcaaccgc tgcgccgagg atcctcacta acttttgaca gaagaagatc aaaagcaaga
     6481 tgaagagtat cgccagctgg cttttgagcg tctcccgcac cagatatctt atgattatca
     6541 ctttaaatac gcccgtaaaa actcgtcttt tgcaggattt tagcttgttt catggcttaa
     6601 acgtcattta ttctcttgag tcgtcgaaat cgtcgctaag ataattatac tcaacggatt
     6661 cacctctcag attttgttct gacgtgccaa tgccgtaata acgttaagat taacacgaag
     6721 tcatcgcaac agcggacatg agttacgaaa gcttgcaatt ctatctgtag ccaccgccgt
     6781 tgtctttaag attcaggagc gtagtgcatg gagtttagtg taaaaagcgg tagcccggag
     6841 aaacagcgga gtgcctgcat cgtcgtgggc gtcttcgaac cacgtcgcct ttctccgatt
     6901 gcagaacagc tcgataaaat cagcgatggg tacatcagcg ccctgctacg ccggggcgaa
     6961 ctggaaggaa aaccggggca gacattgttg ctgcaccatg ttccgaatgt actttccgag
     7021 cgaattctcc ttattggttg cggcaaagaa cgtgagctgg atgagcgtca gtacaagcag
     7081 gttattcaga aaaccattaa tacgctgaat gatactggct cgatggaagc ggtctgcttt
     7141 ctgactgaac tgcacgttaa aggccgtaac aactactgga aagttcgtca ggctgtcgag
     7201 acagcaaaag agacgctcta cagtttcgat cagttgaaaa cgaataagag cgaaccgcgt
     7261 cgtccgctgc gtaaaatggt gttcaacgtg ccgacccgcc gtgaactgac cagcggtgag
     7321 cgcgcgatcc agcacggtca ggcgattgcc gccgggataa aagcagcgaa agatctcggt
     7381 aatatgccgc cgaatatctg taacgccgct tatctcgcct ctcaggcgcg ccagctggct
     7441 gacagctaca gcaagaatgt catcacccgc gttatcggcg aacagcagat gaaagagctg
     7501 ggaatgcact cttatctggc ggtcggtcag ggttcacaaa acgaatcgct gatgtcggtg
     7561 attgagtaca aaggcaacgc gtcggaagat gcacgcccaa tcgtgctggt gggtaaaggt
     7621 ttaaccttcg actccggcgg tatctccatc aagccttcag aaggcatgga tgagatgaag
     7681 tacgatatgt gcggcgcggc ggcagtttac ggcgtgatgc gtatggtcgc ggagctgcaa
     7741 ctgccgatta acgttattgg cgtgctggca ggctgtgaaa acatgcctgg cgggcgtgcc
     7801 tatcgtccgg gcgatgtgtt aaccaccatg tccggtcaaa ccgttgaagt gctgaacacc
     7861 gatgccgaag gccgcctggt actgtgcgac gtgttaactt acgttgaacg ttttgagccg
     7921 gaagcagtga ttgacgtggc gacgctgacc ggtgcctgcg tgatcgcgct gggtcatcat
     7981 attactggtc tgatggcgaa ccataatccg ctggcccatg aactgattgc cgcgtctgaa
     8041 caatccggtg accgcgcatg gcgcttaccg ctgggtgacg agtatcagga acagctggag
     8101 tccaattttg ccgatatggc gaacattggc ggtcgtcctg gtggggcgat taccgcaggt
     8161 tgcttcctgt cacgctttac ccgtaagtat aactgggcgc acctggatat tgcaggaacc
     8221 gcctggcgtt ctggtaaagc aaaaggcgca accggtcgtc cggtagcgtt gctggcacag
     8281 ttcctgttaa accgcgctgg gtttaacggc gaagagtaag tttgcgtttt accctcaccc
     8341 taaccctctc cctaagggag agggaaccga tcgagcgcaa tagaaattca ttgctcatct
     8401 ttcagggtga ggaatgcagc aatatttaaa tccaccacaa gaagccccat atatgaaaaa
     8461 cgcaacgttc taccttctgg acaatgacac caccgtcgat ggcttaagcg ccgttgagca
     8521 actggtgtgt gaaattgccg cagaacgttg gcgcagcggt aagcgcgtac tcatcgcctg
     8581 tgaagatgaa aagcaggctt accggctgga tgaagccctg tgggcgcgtc cggcagaaag
     8641 ctttgttccg cataatttag cgggagaagg accgcgcggc agtgcgccgg tggagatcgc
     8701 ctggccgcaa aaacgcagca gcagcccgcg cgatatattg attagtctgc gaacaagctt
     8761 tgcagatttt gccaccgctt tcacagaagt ggtagacttc gttccttatg aagattctct
     8821 gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg gctggtttca acctgaatac
     8881 ggcaacctgg aaataatgga aaagacatac aacccacaag atatcgaaca gccgctttac
     8941 gagcactggg aaaagcaggg ctactttaag cctaatggcg atgaaagcca ggaaagtttc
     9001 tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc atatgggtca cgccttccag
     9061 caaaccatca tggataccat gatccgctat cagcgcatgc agggcaaaaa caccctgtgg
     9121 caggtcggta ctgaccacgc cgggatcgct acccagatgg tcgttgagcg caagattgcc
     9181 gcagaagaag gtaaaacccg tcacgactac ggccgcgaag ctttcatcga caaaatctgg
     9241 gaatggaaag cggaatctgg cggcaccatt acccgtcaga tgcgccgtct cggcaactcc
     9301 gtcgactggg agcgtgaacg cttcaccatg gacgaaggcc tgtccaatgc ggtgaaagaa
     9361 gttttcgttc gtctgtataa agaagacctg atttaccgtg gcaagcgcct ggtaaactgg
     9421 gacccgaaac tgcgtaccgc tatttctgac ctggaagtgg aaaaccgcga atcgaaaggt
     9481 tcgatgtggc acatccgcta tccgctggct gacggcgcga aaaccgcaga cggtaaagat
     9541 tatctggtgg ttgcgaccac tcgcccggaa accctgctgg gcgataccgg tgtagctgtt
     9601 aacccggaag atccgcgtta caaagatctg attggcaaat atgtcattct gccgctggtt
     9661 aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca tggaaaaagg caccggctgc
     9721 gtgaagatca ccccggcgca cgactttaac gactatgaag tcggtaaacg tcacgccctg
     9781 ccgatgatca acatcctgac ctttgacggt gatatccgtg aaagcgccca ggtgttcgat
     9841 accaaaggta acgaatctga cgtttattcc agcgagatcc cggcagagtt ccagaaactc
     9901 gagcgttttg ctgcacgtaa agcagtcgtt gctgcggttg atgcactcgg cctgctggaa
     9961 gaaattaaac cgcacgacct gaccgttcct tacggcgacc gtggcggcgt ggttatcgaa
    10021 ccgatgctga ccgaccagtg gtacgtgcgt gccgatgtac tggcgaaacc ggcagttgaa
    10081 gcggttgaga acggcgacat tcagttcgta ccgaagcagt acgaaaacat gtacttctcc
    10141 tggatgcgcg atattcagga ctggtgtatc tctcgtcagc tgtggtgggg tcaccgtatc
    10201 ccggcatggt atgacgaagc gggtaatgtt tatgttggcc gcaacgaaga agaagtgcgc
    10261 aaggaaaata acctcggtgc caacgttgcc ctgcgtcagg acgaagacgt tctcgacacc
    10321 tggttctctt ctgcactgtg gaccttctct acccttggct ggccggaaaa taccgacgcc
    10381 ctgcgtcagt tccacccaac cagcgtgatg gtatctggtt tcgacatcat cttcttctgg
    10441 attgcccgca tgatcatgat gaccatgcac ttcatcaaag atgaaaatgg caaaccgcag
    10501 gtgccgttcc acactgttta catgaccggt ctgattcgtg acgacgaagg tcagaagatg
    10561 tccaaatcca agggtaacgt tatcgacccg ctggatatgg ttgacggtat ttcgctgcca
    10621 gaactgctgg aaaaacgcac cggcaatatg atgcagccgc agctggcgga caaaatccgt
    10681 aagcgcaccg agaaacagtt cccgaacggc attgagccgc acggcaccga tgccctgcgc
    10741 ttcaccctgg cggcgctggc gtctaccggt cgtgacatca actgggatat gaagcgtctg
    10801 gaaggctacc gtaacttctg taacaagctg tggaacgcca gccgctttgt gctgatgaac
    10861 acagaaggtc aggattgcgg cttcaacggc ggcgaaatga cgctatcgct ggcggaccgc
    10921 tggattctgg cggagttcaa ccagactatc aaagcgtacc gcgaagcgct ggacagcttc
    10981 cgcttcgata tcgccgcagg cattttgtat gagttcacct ggaaccagtt ctgcgactgg
    11041 tatctggagc tgaccaagcc ggtaatgaac ggtggaacag aagccgaact gcgcggtact
    11101 cgccatacgc tggtgactgt actggaaggt ctgctgcgcc tcgcgcatcc gatcattccg
    11161 ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt gcggtatcac tgccgacacc
    11221 atcatgctgc agccgttccc gcagtatgat gcatctcagg ttgatgaagc cgcactggcc
    11281 gacaccgagt ggctgaaaca ggcgatcgtt gctgtacgta acatccgtgc agaaatgaac
    11341 atcgcgccgg gcaaaccgct ggagctgctg ctgcgtggtt gcagcgcgga tgcagaacgt
    11401 cgcgtaaatg aaaaccgtgg cttcctgcaa acgctggcgc gtctggaaag catcaccgtg
    11461 ctgcctgccg atgacaaagg tccggtttcc gttaccaaga tcatcgacgg cgcagagctg
    11521 ctgatcccga tggctggcct catcaacaaa gaagatgagc tggcgcgcct ggcgaaagaa
    11581 gtggcgaaga tcgaaggtga aatcagccgc atcgagaaca agctggcaaa tgaaggcttt
    11641 gtcgcccgcg caccggaagc ggttatcgcg aaagagcgtg agaagctgga aggttatgca
    11701 gaagcgaaag ccaagctgat tgaacagcag gctgttatcg ccgcactgta atcaacacag
    11761 tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag aaatggtaaa
    11821 gaaggcataa ctccccgaga aatactggct aagaagcctt tatctggttg ttcttcttta
    11881 ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc ctgccagtca
    11941 atcatccata ttttcagcag tgggtaagcc agaccacccg taatcccgga tatcaccacc
    12001 aacgcacaca tgcgataaag cataccgtgg tacgttaaag ttgagcgaaa acgaatacgc
    12061 ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt taaataactg
    12121 gtgctaacag caatcccaaa ataataaatt aactgcgcga ttatcatttt acgttgcatt
    12181 tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact atcatactca
    12241 tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa taaagctaaa
    12301 aatgcgagga tggcatattt tatacaatac gcagtatcta atttcacctt gaaacgatgt
    12361 ataccgaaac tggtattgct ccagagaaaa ctcattacca ggctatataa agtaccatta
    12421 aaaataccaa tggaaacaat accaactatt gccatcagaa ccacagatat aataacacta
    12481 cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaatg
    12541 gccattaaaa tcggcaagaa aaaggttacc caccagaacc ctttcaaaga gcaattaaaa
    12601 ctaaatctta cgccgttgag actggtcatt aaggcctgat aacgcagtcc ttttgctgcc
    12661 ataaatataa gcaaaaccaa cagtaacaaa gtcaaaacac agccaacaag cggcatatct
    12721 gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa
    12781 acattcccac cggtaattcc ataagaaaat cgttgtccgt taacttccat attagcataa
    12841 agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt tataatcgtt
    12901 aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaaa
    12961 gaatgggacg gaacatccat ttcattaata acttgagcca taaaaatccc ctgaattaaa
    13021 aaatccattg atataattta tagatacaga cagtaaaatg ttctgccacg ttacaatact
    13081 cttatcagta ataggctggc aaaaacttta ttgctttata agacgcttgc gcacgtaaga
    13141 agagagtggt attaaacaaa tgtataatta ctttattttt attgggttaa actatgaata
    13201 acattgcgcc gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg
    13261 ccagcgtcat ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg
    13321 ttgccgatcc gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt
    13381 gggtggttga atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta
    13441 gtgaagcgga catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag
    13501 ggctagcgaa aaaactggcc ttaatggcga tggagcaggc acgagagatg ggcttcaaac
    13561 gctgctatct ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg
    13621 gctttgagca tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga
    13681 tgctacgcga actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt
    13741 aattagtggc gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg
    13801 ccgttaggat cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc
    13861 agcgtcatca gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg
    13921 aggatgtcgc agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc
    13981 acttcgtaac cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct
    14041 gcggaaagat ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat
    14101 tcttcaataa tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg
    14161 gctcattcct cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc
    14221 cacctttgtg acaaagattt atgctttaga cttgcaaatg aataataata cacataaatt
    14281 gagttttaat tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa
    14341 gcatttcctg aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc
    14401 cgcgaagctg aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa
    14461 catcgcgctc atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc
    14521 atatgaccag ggggctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa
    14581 agagtcgatt aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg
    14641 cggctatggt caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa
    14701 cggcctgacc aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga
    14761 gcatttaccc ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa
    14821 caacatgggc aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt
    14881 cgcgccacag gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca
    14941 acaaaatggc gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga
    15001 ctttatctat accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg
    15061 cattgcatta ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc
    15121 ggaggtcaaa ttcctccatt gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa
    15181 aaaaatggcg gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga
    15241 atctgccgcc agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt
    15301 gatggtcgcg acgctcagta aattaaa
//
LOCUS       DAJTOW010000090        15036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTOW010000090 DAJTOW010000000
VERSION     DAJTOW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.090"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..176)
                     /locus_tag="OO244_003030"
     CDS             complement(<1..176)
                     /locus_tag="OO244_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664770.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OO244_003031"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OO244_003031"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5664771.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OO244_003032"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OO244_003032"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5664772.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="OO244_003033"
     CDS             complement(2012..2884)
                     /locus_tag="OO244_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5664773.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OO244_003034"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OO244_003034"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5664774.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="OO244_003035"
     CDS             complement(3576..4361)
                     /locus_tag="OO244_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5664775.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OO244_003036"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OO244_003036"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5664776.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="OO244_003037"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="OO244_003037"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5664777.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLCGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="OO244_003038"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="OO244_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5664778.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="OO244_003039"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="OO244_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5664779.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGDTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="OO244_003040"
     CDS             8653..9555
                     /locus_tag="OO244_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5664780.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="OO244_003041"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="OO244_003041"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5664781.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVYQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYHADSEWAALFASLNSI"
     gene            11836..13196
                     /locus_tag="OO244_003042"
                     /pseudo
     CDS             11836..13196
                     /locus_tag="OO244_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13239..14642
                     /locus_tag="OO244_003043"
     CDS             13239..14642
                     /locus_tag="OO244_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5664782.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14710..>15036
                     /gene="ompL"
                     /locus_tag="OO244_003044"
     CDS             14710..>15036
                     /gene="ompL"
                     /locus_tag="OO244_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5664783.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3690 a         3870 c         3744 g         3732 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatc tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaag tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 atcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcgc tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctctgcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcgc ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gagatacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaaccaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtaccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc acgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcccaacgaa cgcgcctcac tggcggcatg
    12301 gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg gtgggattcg tgccagttat
    12361 gaatcttatc gaaggtaatc agaaacttgg ctatatcttc gccgccacgc tgttttcact
    12421 gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg aaagagcgtt atgtcgaaac
    12481 ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa tctttccgcg caattgccgg
    12541 taaccgccca ctgttcattc tgtgtattgc caacctctgc accttagggg cgtttaacgt
    12601 caagctcgcc attcaggtct attacaccca gtacgtactt aacgatccca tcctgttgtc
    12661 gtatatggga tttttcagca tgggctgtat tttcatcggc gtgttcctga tgcctggcgc
    12721 agtcagacgt tttggtaaga agaaggtcta tatcggcggc ctgctgattt gggtgctggg
    12781 cgatctgctc aactatttct tcggcggcgg ctcggtcagc tttgtggcgt tctcctgcct
    12841 ggcgttcttc ggctcagcgc ctgaactggg cgctggtttc cgacaccgtc gagtacggcg
    12901 agtggcgcac cggcgtgcgt tcggaaggca cggtctacac cggcttcacc ttctttcgca
    12961 aagtgtctca ggcgctggct ggtttcttcc ccggctggat gctgacgcaa attggctatg
    13021 tgccgaacgt cgcacaggct gaccacacca ttgaagggtt acgccagttg atcttcatct
    13081 acccaagcgc actggcggta gtcaccattg tggcgatggg ttgcttctac agcctgaacg
    13141 agaagatgta tgtccgcatt gtggaagaga tagaagcccg taaacgcacg gcgtaataat
    13201 aataaacgac gccctgcggg gcgttataag gagtgattat gtctgaccat aatccactga
    13261 cattaaaact gaatctgcgg gaaaaaatcg cctatggtat gggcgacgtc ggttcgaatt
    13321 taatgctctg catcggtact ctgtatctcc tcaaacttta taccgatgaa ttagggatgc
    13381 ctgcttacta tggcgggatt atctttctgg tcgcgaagtt ttttaccgcg tttaccgata
    13441 tgctcaccgg atttttactc gactcgcgga aaaatattgg gcctaaaggc aaatttcgtc
    13501 cctttatttt atatgcagca gttccggcgg cgttaattgc aacgctccag tttatcgcca
    13561 ccaccttttg tttgccggtt aaaacgacga ttgccaccac gctgtttatg atgtttggac
    13621 tttcatacag tctgatgaac tgctcgtatg gtgcgatgat cccggcaatt accaaaaacc
    13681 cgaatgagcg cgcgcagctc gcggcttacc gccagggtgg tgcaaccata gggctattga
    13741 tttgtaccgt ggcgtttatt ccgttgcagt cgcttttttc tgactcaacc gtcggttatg
    13801 cctgtgcggc acttatgttc tccattggcg gctttatttt tatgatgctg tgctacagag
    13861 gcgtcaaaga gcattatgtg gacacagcgc caaccggaca taaagccagt attctcaaat
    13921 ctttttgcgc gatatttcgt aatccaccat tgctggtttt atgcattgct aacctgtgta
    13981 ctctggcggc atttaatatc aaactggcga ttcaggtcta ttacacccag tatgtgctga
    14041 atgatattaa tttattgtcg tggatgggat tcttcagtat gggatgcatc ctcgtcggcg
    14101 tattactggt gccagtaact gtaaaatgtt ttggtaaaaa acaggtttat ttagctggca
    14161 tggtgctatg ggcggtgggt gatatactga attatttctg gggaagtaac tctttcactt
    14221 tcgtcatgtt ctcttgtgtc gccttttttg gcacggcctt tgtaaacagc ctgaactggg
    14281 cactggtacc agataccgta gattacggtg aatggaaaac cggtattcgt gccgaaggtt
    14341 ctgtttatac cggttatacc ttctttcgta aaatttctgc cgcacttgct ggcttcttgc
    14401 caggcattat gctgacgcaa attggttatg ttcccaacat cgcgcaaagc gatgcgacat
    14461 tacagggttt gcgtcagctc atttttatct ggccttgtgc actggcaatt attgctgcat
    14521 tgacgatggg attcttttac acactcaatg aaaaacggtt tgcattgatt attgaggaaa
    14581 tcaaccaacg caaaaataaa gagatggcga cagaaaaaaa aacggcttcc gtaacgttat
    14641 aaatgtcaat agccgctatt tccatcctgg tgggtggcgg cctccctacg tttaaaaaat
    14701 ggacttatta tgaaaaagat taatgcaata attttattat cttctctgac ctctgcctcg
    14761 gtatttgctg gcgcatacgt cgaaaacagg gaggcatata atcttgcctc cgatcagggc
    14821 gaggttatgc tacgtgtggg ttataacttc gatatgggcg cgggtattat gttaaccaat
    14881 acctacacct ttcagcgaga agatgaacta aaacatggct ataatgaaat tgaaggttgg
    14941 tatcctttat tcaaaccaac cgataaatta accatccagc ccggtggctt aattaatgat
    15001 aagagtatca gttcgggtgg tgcagtgtat ctggac
//
LOCUS       DAJTOW010000091        15008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTOW010000091 DAJTOW010000000
VERSION     DAJTOW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.091"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..251
                     /locus_tag="OO244_003045"
     CDS             <1..251
                     /locus_tag="OO244_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5664784.1"
                     /translation="IVTALSGIQRTSMNSLASRNQYQQLWRHGWQQTQLRAISPPANW
                     QVNRMQTSQAGCVSISVTLVSPGGREGEMTRLHCPNRQ"
     gene            264..3632
                     /gene="recC"
                     /locus_tag="OO244_003046"
     CDS             264..3632
                     /gene="recC"
                     /locus_tag="OO244_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5664785.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            3808..6696
                     /gene="ptrA"
                     /locus_tag="OO244_003047"
     CDS             3808..6696
                     /gene="ptrA"
                     /locus_tag="OO244_003047"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5664786.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            6689..10231
                     /gene="recB"
                     /locus_tag="OO244_003048"
     CDS             6689..10231
                     /gene="recB"
                     /locus_tag="OO244_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5664787.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            10231..12057
                     /gene="recD"
                     /locus_tag="OO244_003049"
     CDS             10231..12057
                     /gene="recD"
                     /locus_tag="OO244_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5664788.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RIWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12119..13450)
                     /gene="argA"
                     /locus_tag="OO244_003050"
     CDS             complement(12119..13450)
                     /gene="argA"
                     /locus_tag="OO244_003050"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5664789.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            13682..14935
                     /gene="amiC"
                     /locus_tag="OO244_003051"
     CDS             13682..14935
                     /gene="amiC"
                     /locus_tag="OO244_003051"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5664790.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         3554 a         3788 c         4182 g         3484 t
ORIGIN      
        1 tgattgtcac tgcgttatcg ggtatccagc gaacatcaat gaacagtctc gccagcagaa
       61 accagtacca acagctctgg cggcatggtt ggcagcaaac gcaactgcgc gcgatttcgc
      121 cacctgccaa ctggcaggtc aaccgaatgc agacatcgca ggcgggatgt gtcagcatca
      181 gcgttacgct agtttcaccc gggggcagag aaggcgagat gacccgcctg cattgcccga
      241 atcgtcagta gtcaggagcc gctatgttaa gggtctacca ttccaatcgt ctggacgtgc
      301 tggaagcgtt gatggagttt attgtcgaac gcgaacggct ggacgatcct ttcgaaccag
      361 agatgattct ggtgcaaagt accggtatgg cgcagtggct gcaaatgacc ctgtcgcaaa
      421 agtttggtat tgcggcaaac attgattttc cgctgccagc gagctttatc tgggatatgt
      481 tcgtccgggt gttaccggaa atccccaaag agagcgcctt taacaaacag agcatgagct
      541 ggaaactgat gactctgctg ccgcaattgc tggagcgcga agactttacc ctgttgcggc
      601 attatctgac tgacgatagc gacaagcgaa aactgttcca gctttcctca aaagcggcgg
      661 acctgtttga ccagtatctg gtctatcgtc cggactggct ggcacagtgg gaaacaggac
      721 atttggttga agggctggga gaagcacagg cctggcaagc cccgttgtgg aaggcgctgg
      781 tggaatatac ccatgaactc ggacaaccgc gctggcaccg cgcgaatctc tatcagcgct
      841 ttatcgaaac gctggagtcc gcgacgacct gcccgccggg gttaccttcg cgcgtcttta
      901 tatgcggtat ttccgcgtta ccgcctgttt atctccaggc gctccaggcg ctgggtaaac
      961 atattgaaat ccatctcctg tttaccaacc cctgccgtta ttactggggc gatattaaag
     1021 atcctgctta tctggcgaaa ctgctgactc gtcagcgtcg acacagtttt gaagatcgcg
     1081 aattgccgct atttcgcgat agcgaaaatg ccggacagct ctttaacagc gatggtgaac
     1141 aggatgtcgg caactcgctg ctggcctcat gggggaagct tgggcgcgac tacatttatc
     1201 tcctttctga cctggagagc agccaggagc tggacgcttt tgtcgatgtg acgccagata
     1261 acctgctgca taacattcag tctgacattc tggaactgga aaaccgcgcc gttgcgggtg
     1321 tgaacatcga agagttttcc cgtagcgata acaaacgccc gcttgatcca ctggatagca
     1381 gtatcacatt ccacgtttgc catagcccgc agcgtgaagt tgaagtttta cacgatcgct
     1441 tgctggcgat gctggaggaa gccccgacac ttactccgcg cgacatcatc gtgatggtgg
     1501 ctgatatcga cagctacagt ccgtttattc aggcagtgtt tggtagcgca cctgcggatc
     1561 gttacctgcc ttacgccatt tccgaccgtc gtgcgcggca gtcacatccg gtactggaag
     1621 cgtttatcag cctgttatca ctgcctgaca gtcgctttgt gtcagaggat gtgctggcgc
     1681 tgctggatgt gccggtgctg gcggcgcggt ttgacatcac cgaagaaggg ctgcgttatt
     1741 tacgccagtg ggtcaacgaa tccggcattc gttgggggat agatgacgac aacgttcgcg
     1801 agctggaact ccccgccacc ggacaacaca cctggcgatt tggcctgacg cgtatgttgc
     1861 tgggctacgc gatggagagc gcgcagggcg agtggcaatc ggttctacct tatgatgaat
     1921 cgagcggctt aattgcagaa ctggtggggc atctggcttc actgctaatg cagctaaaca
     1981 tctggcgtcg cgggctggca caggagcgtc cgctagaaga gtggttgccg gtttgtcgcg
     2041 atatgctcaa cgccttcttc ctgccggatg cggaaaccga agcggcgatg acgctgatcg
     2101 aacaacaatg gcaggcgatt atcgccgaag gtttaggtgc gcagtatggc gacgcggtgc
     2161 cgctgtcact attgcgtgat gaactggcac agcgtctgga tcaagaacgt atcagccagc
     2221 gttttctcgc cggaccggtt aacatttgta ctctgatgcc aatgcgttca attccgttca
     2281 aagtggtttg cctgctggga atgaacgacg gcgtttatcc acgtcagctt gcgccattgg
     2341 gctttgacct gatgagccag aaaccgaagc gtggcgaccg tagccgtcgc gatgacgacc
     2401 gctacctgtt cctggaagcg ttaatttccg cgcagcaaaa actctatatc agctatatcg
     2461 ggcgttccat tcaggataac agtgaacgtt tcccgtcggt actggtgcag gaactgatcg
     2521 actacatcgg gcaaagtcat tatctaccgg gcgatgaagc gctcaactgt gatgaaagcg
     2581 aggcaagggt aaaagcgcat cttacttgcc tccatacccg gatgccgttt gatccgcaaa
     2641 actaccagcc aggcgaacga caaagctatg ctcgtgaatg gctacctgcg gccagccagg
     2701 ctggtaaagc acattctgaa tttgttcagc cgctgccgtt taccttaccg gaaaccgtgc
     2761 cgctggaaac gctacaacga ttctgggcac atccggtgcg ggcgttcttc cagatgcgtt
     2821 tgcaggtgaa cttccatact gaagacagcg aaatcccaga caccgagcca tttattctgg
     2881 aaggacttag ccgttatcaa atcaatcagc agttattgaa tgcactggtt gagcaggatg
     2941 atgccgaacg cttgttccgc cgcttccggg cggcagggga tttaccgtat ggcgcttttg
     3001 gtgaaatttt ctgggaaaca cagtgccagg agatgcagca gcttgccgac agagtcattg
     3061 cctgtcgcca gccggggcag agtatggaaa ttgatctcgc ctgcaacggt gtgcagataa
     3121 ctggctggtt gccgcaggtg cagccggatg gcctgttgcg ctggcgtccc tctttattaa
     3181 gtgtggcgca gggaatgcaa ctttggctgg aacaccttgt ctactgtgcc agcggtggta
     3241 atggcgaaag tcgccttttt ctacgcaaag acggtgagtg gcgttttccg ccgcttgcag
     3301 ccgaacaggc tttgcattac ctctcacaac tgattgaggg gtatcgtgaa ggaatgtccg
     3361 cgccattgct ggtgttacct gaaagtggcg gcgcgtggct aaaaacctgt tatgacgcgc
     3421 aaaacgatgc catgctggat gacgattcca cgttgcaaaa agcccgtacg aaattccttc
     3481 aggcttacga aggcaacatg atggtgcgtg gcgaaggtga tgatatctgg tatcaacggc
     3541 tctggcggca attaacacca gagacaatgg aggccatcgt tgaacagtcg caacgtttcc
     3601 tgttaccgct gtttcgcttt aatcagtcat gagtgttgta taaaaattac gcaatctatc
     3661 tgcttacttt atgatgcgca ccagtcacgg actgatggtt atataaacat aggctgactc
     3721 gtgcagcaca agattaaatt ctggcagatg atttgcgtta acctgttgaa tctgtccaga
     3781 aaataaagtt gattatgagg tccgtgaatg ccccgcagca cctggttcaa agcattattg
     3841 ttgtttgttg ccctctgggc accattaagt caggcagaaa cgggatggca gccgattcag
     3901 gaaaccatcc gtaaaagtga taaagataac cgccagtatc aggctatacg tctggataac
     3961 ggtatggtgg tcttgctggt ttctgatccg caggcagtta aatcgctctc ggcgctggta
     4021 gtgcccgttg ggtcgctgga agatcccgag gcgtaccagg ggctggcaca ttaccttgaa
     4081 catatgagtc tgatggggtc gaaaaagtac ccgcaggctg acagtctggc cgaatatctc
     4141 aaaatgcacg gcggtagtca caatgccagc actgcgccgt atcgcacggc tttctatctg
     4201 gaagttgaga acgacgcctt gcccggtgcg gtagaccgcc tggccgatgc tattgcagaa
     4261 cccttgctcg acaagaaata cgccgaacgt gagcgtaatg cagtgaacgc tgaattaacc
     4321 atggcgcgta cgcgtgacgg gatgcgcatg gcacaggtca gcgcagaaac cattaacccg
     4381 gcacaccccg gttcaaagtt ttctggtggt aacctcgaaa ctttaagcga caaacctggt
     4441 aatccggtgc agcaggcgct gaaagatttc cacgagaagt actattccgc caatttgatg
     4501 aaggcggtta tttacagtaa taaaccgctg ccggagttgg caaaaatggc ggcggacacc
     4561 tttggtcgcg tgccgaacaa agagagcaaa aaaccggaaa tcaccgtgcc ggtagtcacc
     4621 gacgcgcaaa agggcattat cattcattac gtccctgcgc tgccgcgtaa agtgttgcgc
     4681 gttgagtttc gcatcgataa caactcagcg aagttccgta gtaaaaccga tgaattgatt
     4741 acctatctga ttggcaatcg cagcccaggt acactttctg actggctgca aaagcaggga
     4801 ttagttgagg gcattagcgc caactccgat cctatcgtca acggcaacag cggcgtatta
     4861 gcgatctctg cgtctttaac tgataaaggc ctggctaatc gcgatcaggt tgtggcggca
     4921 atttttagct atctcaatct gttacgtgaa aaaggcattg ataaacaata cttcgatgaa
     4981 ctggcgaatg tgctggatat cgacttccgt tatccgtcga tcacccgtga tatggattac
     5041 gtcgaatggc tggcagatac catgattcgc gttcctgttg agcatacgct ggatgcagtc
     5101 aatattgccg atcggtacga tgctaaagca gtaaaggaac gtctggcgat gatgacgccg
     5161 cagaatgcgc gtatctggta tatcagcccg aaagagccgc acaacaaaac ggcttacttt
     5221 gtcgatgcgc cgtatcaggt cgataaaatc agcgcacaaa ctttcgccga ctggcagaaa
     5281 aaagccgccg acattgcgct ctctttgcca gagcttaacc cttatattcc tgatgatttc
     5341 tcgctgatta agtcagagaa gaaatacgac catccagagc tgattgttga tgagtcgaat
     5401 ctgcgcgtgg tgtatgcgcc aagccgttat tttgccagcg agcccaaagc tgatgtcagc
     5461 ctgattttgc gtaatccgaa agccatggac agcgcccgca atcaggtgat gtttgcgctc
     5521 aatgattatc tcgcagggct ggcgcttgat cagttaagca accaggcgtc ggttggtggc
     5581 ataagttttt ccaccaacgc taacaacggc cttatgatta atgctaatgg ttacacccag
     5641 cgtctgccgc agctgttcca ggcattgctc gaggggtact ttagctatac cgctacggaa
     5701 gagcagcttg agcaggcgaa gtcctggtat aaccagatga tggattccgc agaaaagggt
     5761 aaagcgtttg agcaggcgat tatgcccgcg cagatgctct cgcaagtgcc gtacttctcg
     5821 cgagatgaac ggcgtaaaat tttgccctcc attacgttga aagaggtgct ggcctatcgc
     5881 gacgccttaa aatcaggggc tcgaccagag tttatggtta tcggcaacat gaccgaggcc
     5941 caggcaacaa cgctggcacg cgatgtgcaa aaacagttgg gcgctgatgg ttcagagtgg
     6001 tgtcgaaaca aagatgtagt ggtcgataaa aaacaatccg tcatctttga aaaagccggt
     6061 aacagcaccg actccgcact ggcagcggta tttgtaccga ctggctacga tgaatacacc
     6121 agctcagcct atagctctct gttggggcag atcgtacagc cgtggttcta caatcagttg
     6181 cgtaccgaag aacaattggg ctatgccgtg tttgcgtttc caatgagcgt ggggcgtcag
     6241 tggggcatgg gcttcctttt gcaaagcaat gataaacagc cttcattctt gtgggagcgt
     6301 tacaaggcgt ttttcccaac cgcagaggca aaattgcgag cgatgaagcc agatgagttt
     6361 gcgcaaatcc agcaggcggt aattacccag atgctgcagg caccgcaaac gctcggcgaa
     6421 gaagcatcga agttaagtaa agatttcgat cgcggcaata tgcgcttcga ttcgcgtgat
     6481 aaaatcgtgg cccagataaa actgctgacg ccgcaaaaac ttgctgattt cttccatcag
     6541 gcggtggtcg agccgcaagg catggctatt ctgtcgcaga tttccggcag ccagaacggg
     6601 aaagccgaat atgtgcatcc tgaaggctgg aaagtgtggg agaacgtcag cgcgttgcag
     6661 caaacaatgc ccctgatgag tgaaaagaat gagtgatgtc gccgagacac tagatccttt
     6721 gcgcttgccc ttacagggcg agcgcctgat tgaagcctct gccggcacag gcaaaacctt
     6781 tacgattgcg gcgctctatt tgcgcctgtt acttggacta ggcggttccg ccgcctttcc
     6841 ccgcccgctg atcgttgaag aactgctggt ggtcaccttt accgaggctg ccacggcaga
     6901 attgcgcggt cgtatccgta gcaatatcca cgagttgcgc atcgcctgtc tgcgtgaaac
     6961 caccgacaat ccactgtacg aacgcctgct ggaagagatc gacgataaag cgcaagccgc
     7021 gcagtggttg ttgttagccg aacggcagat ggatgaagcg gcagtcttta ctattcacgg
     7081 cttttgccag cgcatgctca acctgaatgc ctttgaatcc ggcatgctgt ttgagcagca
     7141 gctgattgaa gatgagtctc tgctacgcta ccaggcctgc gccgatttct ggcgtcgcca
     7201 ctgctacccg ctgccgcgtg aaatagccca ggtcgtcttt gaaacctgga aagggccgca
     7261 ggcgttgctg cgcgatatta atcgttatct gcaaggcgaa ccgccggtta tcaaagcacc
     7321 gccgcccgat gatgaaacgc tggcttcccg ccacgcgcaa attgtggcgc gtattgatac
     7381 ggtaaaacag cagtggcgcg acgcagtggg tgaactggat gcgctgatcg aatcttctgg
     7441 tattgatcga cgcaagttta accgtagcaa tcaggctaaa tggatcgaaa agatcagcgc
     7501 ctgggcagaa gaagagacaa acagttatca gttgccggag tcgctggaaa aattctccca
     7561 gcgtttctta gaagatcgca cgaaggccgg gggggaaacc ccgcgacatc cactgtttga
     7621 ggcgatcgat caactgcttg cagaaccatt gtcgatccgc gatctgttga tcacccgcgc
     7681 attggctgag atccgcgaaa cagtagcgcg tgaaaaacgc cgccgtggcg aattgggttt
     7741 tgatgacatg ttaagtcggc tcgattccgc gctgcgtagc gaaagcggcg aggtgttggc
     7801 agcggcgatc cgtacgcgat tcccggtggc aatgatcgat gaatttcagg ataccgaccc
     7861 ccagcagtac cgaatttttc gccgtatctg gcaccatcag ccggaaaccg cattgttgct
     7921 gattggcgac ccgaagcagg ccatatatgc attccggggt gcggatatct tcacttatat
     7981 gaaggcgcgt agtgaagttc atgcccacta cactttagac accaactggc gctccgcacc
     8041 tggaatggtg aacagtgtga acaagctttt cagccagact gatgacacgt tcatgtttcg
     8101 cgaaataccg tttattccag tgaaatcagc cgggaaaaat caggcgttac gttttgtatt
     8161 taaaggtgaa acgcagcctg cgatgaaaat gtggctgatg gaaggcgaaa gctgcggcgt
     8221 tggcgactat caaagtacca tggcgcaggt atgtgctgcg caaatccgcg actggctaca
     8281 agccggacag cggggcgaag cgttgctgat gaacggcgac gacgcgcgtc cggtgcgtgc
     8341 ttcggacatc agcgtgctgg tgcgcagccg ccaggaggcc gcccaggtgc gcgatgcctt
     8401 aacgttgctg gaaatccctt ccgtttacct ttcgaaccgc gacagtgttt ttgaaactct
     8461 ggaagcgcag gaaatgcttt ggttgttgca ggcggtgatg acgcccgaac gtgagaacac
     8521 cctgcgcagt gcgctggcaa cgtcaatgat ggggctgaat gcgctggata ttgaaacgct
     8581 gaataatgac gaacatgcgt gggatgcggt agtcgaagag ttcgatggtt atcggcaaat
     8641 ctggcgcaaa cgtggcgtta tgccgatgct gcgggcgctg atgtcggcgc gtaacattgc
     8701 agaaaacttg ctggcaacgg caggcggtga gcggcgtctt accgatatct tgcatatcag
     8761 cgaactgctt caagaagccg gaacgcagct ggaaagtgaa catgcgctgg tacgctggtt
     8821 atcgcaacat atcctcgagc cagacagtaa tgcctccagc caacaaatgc gcctcgaaag
     8881 tgataaacat ctggtgcaga ttgtcacgat ccataaatcg aaagggctgg aatatccgct
     8941 ggtctggttg ccatttatta ccaatttccg cgtccaggat caggcgtttt atcacgatcg
     9001 ccactcgttt gaggcagttt tggatcttaa tgctgcgcca gaaagcgtcg acctcgcgga
     9061 ggtcgaacgt ctggcggaag atctgcgttt gctttacgtg gcgctgacgc gttcggtttg
     9121 gcattgcagt ctcggcgttg caccgctggt gcgccgtcgt ggcgataaaa aaggtgacac
     9181 cgacgtccac caaagtgcgc tcgggcgttt gctgcaaaaa ggggaaccgc aagatgcggc
     9241 agggcttcgc acctgtattg aagcgttatg cgatgatgat attgcctggc aaacggcaca
     9301 aactggtgat aaccagccct ggcaggttaa tgatgcttta actgcagaac tgaatgcgag
     9361 gacactacaa cgattgcccg gcgataactg gcgtgtcacc agctactccg gcttgcagca
     9421 gcgtggtcac ggtatcgctc aggatctgat gccacggctg gatgttgatg ccgcaggcgt
     9481 ggtcagcgtc gttgaagaac cgacgttaac accgcatcag ttcccgcgcg gtgcgtcacc
     9541 ggggacattc ttgcacagtt tgtttgaaga cctcgatttt acccagccgg ttgacccgaa
     9601 ctgggtacag gaaaaactgg aactcggcgg ctttgaatcg cagtgggaac cggtgttgac
     9661 cgagtggatc acggctgtcc tccaggcacc tctcaatgaa acgggtgtta gcctgagtca
     9721 gctttccgac cgcgataaac aggtggagat ggagttttac ctgccgatta gcgaaccgct
     9781 catcgccagc cagcttgatg cactaattcg ccagtttgac ccgctatccg ctggctgccc
     9841 gccgctggag ttcatgcagg tacgcggcat gttaaaaggc tttatcgacc tggtattccg
     9901 ccacgaaggg cgttattacc tgctcgacta taaatccaac tggctgggtg aagacagttc
     9961 ggcttacacc caacaggcta tggcagcagc gatgcaggca caccgctatg atctgcaata
    10021 tcagctttat accctggcgc tgcatcgtta tctgcgccat cgcattgctg attacgacta
    10081 tgatcttcac tttggcggcg ttatctatct gttcctgcgt ggcgttgata aagaacatcc
    10141 gcaacaaggg atttacacaa cccgacccaa cgccgggttg attgccctga tggatgagat
    10201 gtttgccggt atgaccctgg aggaggcgta atgaaattgc aaaagcaatt actggaagct
    10261 gtggagcaca aacagctacg cccgctggat gtgcaatttg ccctgaccgt ggcgggagat
    10321 gaacatcctg ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg agagggacac
    10381 gtctgtttgc cgctttcacg actggaaaat aacgaggcgt cgaatccgct gttggcgacc
    10441 tgtgtcagtg aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg
    10501 gtcagcaggg gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat
    10561 cgcatgtggt gtaacgagcg gacggtagcg cgcttcttca atgaggtgaa tcataccatt
    10621 gaggttgatg aagctctact ggcgcaaacc ctggacaaac tttttccagt aagcgatgaa
    10681 attaactggc aaaaagttgc agcggcagtg gcgctgacgc ggcggatctc ggtgatttcc
    10741 ggcggcccag gcaccggtaa aacgaccacc gtggcgaagt tgctggcagc gttaattcaa
    10801 atggccgacg gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg
    10861 cgcttaaccg aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag
    10921 aaacgcattc cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc
    10981 cagcgtttac gtcatcatgc cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa
    11041 gcgtcaatga tcgatctgcc tatgatgtcg agactgatcg acgccttgcc agatcatgcg
    11101 cgagtgatct ttctcggcga tcgtgatcaa ctggcctcgg ttgaggctgg ggctgtgctg
    11161 ggcgatatct gcgcttatgc caacgcgggc tttaccgccg agcgtgccag gcagctaagc
    11221 cgcctgacgg ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac
    11281 agtctctgcc tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta
    11341 gctgcggcga tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt
    11401 actgatatcg aaaaacgact tttacagagc ggcgaagatt atattgcgat gcttgaggaa
    11461 gctcttgcgg gttacggacg ttatctggat ctgctgcaag cgcgtgccga gccggattta
    11521 atcattcagg cgttcaatga gtaccagctt ttgtgcgccc tgcgggaagg gccgtttggc
    11581 gtggctggac tgaatgagcg aattgaacag tttatgcaac agaagcgcaa aattcatcgt
    11641 catccgcact ctcgttggta cgaaggccga ccggtgatga ttgcccgtaa tgacagcgcg
    11701 cttgggttgt ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcatc
    11761 tggtttgcga tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac
    11821 gaaactacgt gggcgatgac ggtacataaa tcgcagggat cggagttcga ccatgcggcg
    11881 ttgattttac cgagccaacg cacgccggta gtaacgcgag agctggtata caccgcggtg
    11941 acccgcgcgc gtcgtcgtct gtcgctgtat gccgatgagc gtatattaag tgcggcaatc
    12001 gccactcgta ctgagcggcg cagtggtctg gcggcgttgt ttagttcgcg ggaataaacg
    12061 taattgccgg atgcgacgca cgagtgttac gcctgccgca tccggtgatt ttcatcgctt
    12121 accctaaatc cgccatcaac actttggatt tacgctggta gttgtacaac tgctttttgc
    12181 tctcgggcag taaatcaata tccaccgggg taaatccacg ttcctggaac cagtgaatac
    12241 tgcgcgtggt cagcacaaac aatttgctta agccgctctg cttcgcctga gcggcaatgc
    12301 gttccagcag aacctcgccc cgtgatgaac tgcggtaatc cgggtgaact gccacacagg
    12361 ccatttcccc aatcttctct tccgggaacg gatagagcgc ggcgcaggca atagtcgtgt
    12421 tatcgcgctg aataatggtg aatttgtcga tttccatctc cagctgctcg cgagaacggc
    12481 gcaccagaat accttgctgc tccagcgggc gaatcaactc cagaataccg ccaatatcgt
    12541 tgattgttgc gcgacgaatc tgctcggcgc tttccatcac aatctgcgta ccgataccgt
    12601 cgcgtgagaa caactcttgc aacagcgcgc catcttcctg ataactgatt aaatgacagc
    12661 ggcgcacgcc gctgcggcag gctttcactg cgccacgcaa aaagcgcacc gtaccggagt
    12721 tgtaatcgcc tttctcttcc tgggcttcta cccgtgcttg cgcttcgtta gggaaaagtt
    12781 cggagacaat atcaccgtcg tcattagtaa cgccctggga agagcaaaaa ccaatcatct
    12841 tttcagcttt cagtttgatg gctagttgag tggcaatctc ttccgaggtc agattaaagc
    12901 tctcgccagt gactgaaaca gcgaccggcc ccattagcac tattgcaccg ctttccagtt
    12961 gacgatggag cgcgtcttca tcaatccgcc ggatacgccc gctatggcag taatccacgc
    13021 cgtcatcgac gcccagcggc tgggcaataa taaaattgcc actaacgacg ttgatatgcg
    13081 cgccctgcag cggcgtgtta ttgagactca ttgacaggcg agcagtaata tccagttgca
    13141 atgttcccgc agcctgcttc accagttcca gtgttttggc gtcggtcaca cgtatattct
    13201 tgtgatacag cggttcgtgg tgatgcgcag ccagatttgc gtcgatctgc ggacgtgcgc
    13261 catagaccac caccagacgg atgccgaggc tgtgcaacaa cccgatatca ttaacgatac
    13321 tggagaaatt ctcatgctca atggcttcac cgccgagcat gatgacaaac gtttttcccc
    13381 ggtgggtatt gatataggga accgaatggc ggaatccctc gaccagctcg gttttacgtt
    13441 cctttaccac ggcacacccc tttgcatgat tatttgaaat tattgtattt ttattctgtt
    13501 ttttcgcagg gtgcaagtgt aaattttatg cgagagcgac ttttttatca gtaatgccgt
    13561 gaatataaaa agaatgttta ccgtttatta gatgacagat tatgcgtctt tcgctaaagt
    13621 ttccggtcaa attagtcgtt tacttgttac acagcttaga tttcttttgc tcgggagagg
    13681 catgtcagga tccaacactg caatcagccg tcgtcgttta ctgcaaggcg cgggcgctat
    13741 gtggctattg agcgtaagtc aggtcagcct ggctgcggtt agccaggtcg tggcggtgcg
    13801 cgtctggcct gcgtccagct acacccgcgt gacggtagaa tcaaatcgtc agctgaaata
    13861 taagcagttc gcgttgagta accctgaacg cgtggtggtg gatatcgaag atgtaaacct
    13921 gaactcggta ctcaagggga tggcggcaca aattcgtgca gacgacccgt tcatcaagtc
    13981 ggcgcgcgtc gggcaatttg acccgcaaac cgtacgcatg gtttttgaat taaagcaaaa
    14041 cgtaaaaccg cagctgtttg cccttgcgcc ggtcgccggg tttaaagagc gtctggtgat
    14101 ggacctctat ccggccaatg cacaggatat gcaggacccg ctgctggcgc tgctggagga
    14161 ttacaacaaa ggcgacctcg aaaagcaggt gccgccagcg caaagtggtc cacaaccggg
    14221 taaagcaggg cgcgatcgtc cgattgtcat tatgcttgac cctggccacg gtggcgaaga
    14281 ctccggtgcg gtggggaaat acaaaacgcg cgaaaaagat gtggtattgc aaatagctcg
    14341 ccgtttgcgc tctctgatcg aggaagaggg caatatgaag gtgtacatga cgcgcaatga
    14401 agacatcttc attccgttgc aagtgcgcgt agcaaaagcc cagaaacagc gtgctgacct
    14461 gtttgtctct atccatgccg acgcctttac cagtcgtcag ccgagcggtt cctcggtgtt
    14521 tgcgctctca accaaaggcg caaccagtac tgcggcaaaa tatctggcac aaacccagaa
    14581 cgcctcggac ttgattggtg gcgtgagcaa aagcggtgac cgctatgtcg accacaccat
    14641 gttcgatatg gtgcagtcgc tgactattgc tgacagcctt aagtttggta aagcggtact
    14701 gaataagctc ggaaaaatca acaagctgca taaaaatcaa gttgaacagg ccgggtttgc
    14761 cgtactaaag gcaccagata ttccctccat tctggtcgaa acggcgttta tcagtaacgt
    14821 tgaggaagag cgtaaactga aaacggcgac tttccagcag gaagttgcgg agtctattct
    14881 tgcggggatt aaagcgtatt ttgccgatgg ggcaacgctg gcgagaaggg gatgataaaa
    14941 aggcgctgaa tggcgctttt tttatgggag atcagaaaca aaaaaacacc cgttagggtg
    15001 ttcaataa
//
LOCUS       DAJTOW010000092        14844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTOW010000092 DAJTOW010000000
VERSION     DAJTOW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.092"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(114..380)
                     /locus_tag="OO244_003052"
     CDS             complement(114..380)
                     /locus_tag="OO244_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5664791.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="OO244_003053"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="OO244_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5664792.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4545..5674
                     /gene="ompN"
                     /locus_tag="OO244_003054"
                     /pseudo
     CDS             4545..5674
                     /gene="ompN"
                     /locus_tag="OO244_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5815..6249
                     /gene="uspF"
                     /locus_tag="OO244_003055"
     CDS             5815..6249
                     /gene="uspF"
                     /locus_tag="OO244_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5664793.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6425..7360
                     /gene="ttcA"
                     /locus_tag="OO244_003056"
     CDS             6425..7360
                     /gene="ttcA"
                     /locus_tag="OO244_003056"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5664794.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO244_003057"
     CDS             complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO244_003057"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5664795.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO244_003058"
     CDS             complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO244_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5664796.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10572..11804
                     /gene="dgcM"
                     /locus_tag="OO244_003059"
                     /pseudo
     CDS             10572..11804
                     /gene="dgcM"
                     /locus_tag="OO244_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO244_003060"
                     /pseudo
     CDS             complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO244_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12642..14096)
                     /locus_tag="OO244_003061"
     CDS             complement(12642..14096)
                     /locus_tag="OO244_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5664797.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14093..14488)
                     /locus_tag="OO244_003062"
                     /pseudo
     CDS             complement(14093..14488)
                     /locus_tag="OO244_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            14601..14789
                     /locus_tag="OO244_003063"
     CDS             14601..14789
                     /locus_tag="OO244_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664798.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT         3937 a         3763 c         3507 g         3637 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaacttct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     4321 cgcagaataa cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga
     4381 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     4441 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     4501 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     4561 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     4621 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     4681 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     4741 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     4801 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     4861 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     4921 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     4981 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     5041 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     5101 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
     5161 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     5221 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     5281 aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc
     5341 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
     5401 ccagtttgat ttttgtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
     5461 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
     5521 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
     5581 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
     5641 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
     5701 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
     5761 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
     5821 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
     5881 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
     5941 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
     6001 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
     6061 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
     6121 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
     6181 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
     6241 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
     6301 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
     6361 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     6421 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     6481 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6541 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6601 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6661 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6721 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6781 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6841 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     6901 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6961 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
     7021 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     7081 acaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     7141 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     7201 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     7261 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     7321 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
     7381 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatctggc
     7441 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     7501 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7561 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7621 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7681 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7741 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7801 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7861 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7921 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
     7981 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
     8041 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
     8101 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
     8161 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
     8221 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
     8281 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     8341 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     8401 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     8461 acgacccggc gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc
     8521 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     8581 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     8641 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     8701 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     8761 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
     8821 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     8881 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     8941 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     9001 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     9061 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     9121 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     9181 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     9241 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     9301 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     9361 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     9421 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     9481 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     9541 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     9601 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     9661 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     9721 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     9781 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     9841 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
     9901 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     9961 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    10021 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    10081 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    10141 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    10201 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    10261 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccactgt
    10321 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    10381 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    10441 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    10501 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    10561 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    10621 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    10681 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    10741 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    10801 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    10861 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    10921 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    10981 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    11041 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    11101 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    11161 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg
    11221 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    11281 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    11341 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    11401 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    11461 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    11521 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    11581 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    11641 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    11701 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    11761 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    11821 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    11881 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    11941 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    12001 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    12061 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    12121 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    12181 acaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    12241 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    12301 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    12361 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    12421 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    12481 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    12541 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    12601 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    12661 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    12721 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    12781 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    12841 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    12901 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    12961 aatgttacga tgggggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac
    13021 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    13081 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    13141 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    13201 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    13261 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    13321 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    13381 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    13441 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    13501 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    13561 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    13621 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    13681 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc
    13741 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    13801 atgatctgaa tattccatgc aagtccacac gcgatactca ttaaagtaaa tattcccgcg
    13861 gatagagtaa aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata
    13921 acaataatct cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg
    13981 gacagaccac cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca
    14041 ataaataatc ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct
    14101 catcaacact gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt
    14161 cgtcaaatgc aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggcat
    14221 tttctatcgg aatcaagctg aaactggctc ctgtcgctgg ggaaaggctt tcaatgtgac
    14281 cgtgaaacac ttcccctttg agaatatcag cacggatctc agccttcatt cctggcttca
    14341 ttcccgctat ttggttttct ttataatttg catcaaccca caactcactg actggtacta
    14401 gtgaaactaa cgatgtgccg ccttccaccc aactgcctgt atgggcgctg cggtttgcga
    14461 caatgccatc aatcggcgca cgaaccacgg cagcctgacg gctgcaaatt gaaaaccgcc
    14521 cctgttcgtc ctgttgttgc cgcacgctcc gcagaatatg tatgctctga ggacctgttt
    14581 tattaaggac tcatcctctt atgttgaccc gcaaaagtat agataccgtt ctgctctctg
    14641 ttggtgctga gaagctctca caacgtgaat gggactggat gaagatgctc aaacccatgg
    14701 acccaccacc tgcgatggtc gcggcctcca tcctggagcg ccgtggcgac acagcagccc
    14761 ttacccggct acaggatacc gggggttaat ccgtcctcat gttgtccgct tttactcccc
    14821 gcccgctgaa acgtctgttc acgg
//
LOCUS       DAJTOW010000093        14704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTOW010000093 DAJTOW010000000
VERSION     DAJTOW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.093"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO244_003064"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO244_003064"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5664799.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO244_003065"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO244_003065"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5664800.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO244_003066"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO244_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5664801.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO244_003067"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO244_003067"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5664802.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO244_003068"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO244_003068"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5664803.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO244_003069"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO244_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5664804.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO244_003070"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO244_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5664805.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSLYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO244_003071"
     CDS             complement(8064..9206)
                     /locus_tag="OO244_003071"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5664806.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO244_003072"
     CDS             complement(9215..10228)
                     /locus_tag="OO244_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5664807.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO244_003073"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO244_003073"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5664808.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO244_003074"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO244_003074"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5664809.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO244_003075"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO244_003075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5664810.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO244_003076"
     CDS             complement(12826..13950)
                     /locus_tag="OO244_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5664811.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..>14704)
                     /gene="rfaQ"
                     /locus_tag="OO244_003077"
     CDS             complement(13947..>14704)
                     /gene="rfaQ"
                     /locus_tag="OO244_003077"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5664812.1"
                     /translation="ILVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNL
                     SVLTPLGLDSLVKQTTMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAK
                     FSAVIDALHARGYEVVLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALID
                     HAQLFIGVDSAPAHIAAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQ
                     RDRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL"
BASE COUNT         4176 a         3321 c         3026 g         4181 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcactat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 gttcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atag
//
LOCUS       DAJTOW010000094        14567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTOW010000094 DAJTOW010000000
VERSION     DAJTOW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.094"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..572
                     /locus_tag="OO244_003078"
     CDS             <1..572
                     /locus_tag="OO244_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5664813.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(634..1356)
                     /locus_tag="OO244_003079"
     CDS             complement(634..1356)
                     /locus_tag="OO244_003079"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5664814.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1358..2533)
                     /locus_tag="OO244_003080"
     CDS             complement(1358..2533)
                     /locus_tag="OO244_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5664815.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(2560..4176)
                     /gene="bglH"
                     /locus_tag="OO244_003081"
     CDS             complement(2560..4176)
                     /gene="bglH"
                     /locus_tag="OO244_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5664816.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4262..5656)
                     /gene="bglB"
                     /locus_tag="OO244_003082"
     CDS             complement(4262..5656)
                     /gene="bglB"
                     /locus_tag="OO244_003082"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5664817.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5675..7552)
                     /gene="bglF"
                     /locus_tag="OO244_003083"
     CDS             complement(5675..7552)
                     /gene="bglF"
                     /locus_tag="OO244_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5664818.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGTALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7686..8507)
                     /gene="bglG"
                     /locus_tag="OO244_003084"
     CDS             complement(7686..8507)
                     /gene="bglG"
                     /locus_tag="OO244_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5664819.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8793..9518)
                     /gene="phoU"
                     /locus_tag="OO244_003085"
     CDS             complement(8793..9518)
                     /gene="phoU"
                     /locus_tag="OO244_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5664820.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9533..10306)
                     /gene="pstB"
                     /locus_tag="OO244_003086"
     CDS             complement(9533..10306)
                     /gene="pstB"
                     /locus_tag="OO244_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5664821.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10398..11288)
                     /gene="pstA"
                     /locus_tag="OO244_003087"
     CDS             complement(10398..11288)
                     /gene="pstA"
                     /locus_tag="OO244_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5664822.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11288..12247)
                     /gene="pstC"
                     /locus_tag="OO244_003088"
     CDS             complement(11288..12247)
                     /gene="pstC"
                     /locus_tag="OO244_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5664823.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12334..13374)
                     /gene="pstS"
                     /locus_tag="OO244_003089"
     CDS             complement(12334..13374)
                     /gene="pstS"
                     /locus_tag="OO244_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5664824.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13622..>14567)
                     /locus_tag="OO244_003090"
     CDS             complement(13622..>14567)
                     /locus_tag="OO244_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5664825.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3753 a         3717 c         3320 g         3777 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag
      601 cgtaattcat acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc
      661 ggctttatca gcgattacca tcagcgaagg atgcagctgc aacacggaag ctggaacatc
      721 ttcggtaacc ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac
      781 aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc
      841 aggcactaac gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc
      901 ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca
      961 aaaatgacca tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag
     1021 tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat
     1081 ccccgcaggg gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt
     1141 gccgcgaaag ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc
     1201 tttaaccagg gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc
     1261 caggttaaca cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct
     1321 catttcctgg taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct
     1381 gtacgaaggc tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt
     1441 cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct
     1501 tgccggttac gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa
     1561 tagctgccgg atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa
     1621 caccactgaa tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg
     1681 cctggtaccc gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacac
     1741 taaaacgctt gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag
     1801 cattcagcgg ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc
     1861 ccttcgcatc ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag
     1921 ccagcaggtt atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccggtat
     1981 caccaaaacc gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtatcctg
     2041 gcggggtcca gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg
     2101 cgatcaaatc accatgcgca acagaacgcg tatccagata actgcctggc accagaatca
     2161 tactggagtt aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta
     2221 caccatcaac attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg
     2281 accagactcc tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca
     2341 ccactacaga gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg
     2401 cattgacctg agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat
     2461 ccgctgctat ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa
     2521 tttttatatt catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg
     2581 ggcaccgaca gcaaactggg cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt
     2641 ttccagggct ttgatatacg tggcgtagaa acgaatttcc agacgcgagg tcaacatact
     2701 ggtattgact ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt
     2761 actattcgca tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata
     2821 ttggtcccaa atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc
     2881 gcctgtttcg tagctataaa tattgttacc gaaggagtaa acaatcgcgt tagccacaat
     2941 aaaatgatcg ccgatatagg cttcgccctg cgaggtcaga cgaactgctg ttccgccggt
     3001 gtgatcacca taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc
     3061 aaagttcctg gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt
     3121 atcaaatttc tgcgttaaag atgtgccgaa catccaggta tctttccagt cataataccc
     3181 gttattatcc tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact
     3241 caccattaag gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatg
     3301 ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac
     3361 cagcgcgata tcaattttac ccggaccgac tttccagttt tccagaccta cacccgctgc
     3421 agcatcagta cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg
     3481 tttacccacc cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta
     3541 catatcggag aactgcataa agttctcgcc accggcatta tcgccaaacc agccggtaga
     3601 gtactgctga ccaacgttac catctatcat cacaacggca tcaacccgtt tgccgttttc
     3661 gttgtagaca cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg
     3721 gcccagagaa ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga
     3781 acggtaatag ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt
     3841 cagagtcatg gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac
     3901 cagtacagca tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg
     3961 atcattcgta cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc
     4021 tttatacttt ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg
     4081 ttgttccacc gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa
     4141 taagatggca gaggtaataa gattttgtct aaacataata tccctttatg gtgcaaagaa
     4201 agaattaacg tatcgcatcc agactgttct gaatgcgacg ataattaagg tgctttattg
     4261 gttatttttt taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt
     4321 tcttacgcgt tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc
     4381 cgtagcgctt agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg
     4441 tgtaccccat aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt
     4501 ttaaataggc aattcgataa tcgtcctgta tcgaaccatc cgcttcaacg ctgtctttta
     4561 cgcctaatcc attctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta
     4621 acagaacccg taatccaacc ggatcaattt gccaccccca ctctgaactt ttcagatgcg
     4681 gattggggat catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg
     4741 aaacacaacc agtcatgtaa taactaaaag agatgaaatc tacagtatgt tttaaatctt
     4801 ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat
     4861 agccgggata ttggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt
     4921 ccatggcctg caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa
     4981 gcatattgcc gattttcgct tcggggatca ggctatgata ggctttaact gcccgcgcac
     5041 tggcaaccag ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca
     5101 gccccacgcc cgtgaatggc gcgtgtaacg acatattgat ttcattaaac gtcagccata
     5161 acgctacttt atgttggtag cgggtaaaga ccgtgcgggc gtaatgctcg aagtgatcga
     5221 tgaccgcccg attagcccaa ccgccgtagt ttttcaccag cccatatagc atttcgtaat
     5281 gggataacgt taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt
     5341 aaaacgctaa ccccgcttca ttcggttcgg cttcgtcgcc ctgagggaaa attcgcgccc
     5401 aggcaatgga aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg
     5461 ggtaacggta ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg
     5521 gttccatttt tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat
     5581 cttccagcca ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt
     5641 ctggaaatgc tttcataatt aactcctttt atcgttagcg aatgatggat aacagcggtt
     5701 cacctgcgct tatctgcgcc gtgccgtggg gtaatacgtc tgtaaaatca tcgctattac
     5761 tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag
     5821 aaatcagccg atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt
     5881 tgccgtccag ttttacggtg tcgataccga catgaatcag gatctccaca ccatcatctg
     5941 actcaatgcc aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag
     6001 aacgcacttc accaaccgag ggcagaatgg cgataccttt acccaacagg ccactggcaa
     6061 acgtggtatc agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac
     6121 cgccctgctc aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg
     6181 gctgacgttt actggtgatg aaatgaagca tcaacgtacc aacaaatgcg caacctatgg
     6241 caatgacacc gccaataacg ctggcccaga cggtgaaatc aatccccgtt gacgggatgg
     6301 tctgcatgaa ggtgaaaata cttggcaaac caaaggagta aactttcgtt tgcgcgtagc
     6361 caatgatggt ggcccccaat gctccactga tacaggcgat aacaaagggg tacttacgcg
     6421 gcaggttgac gccatatacc gctggttcgg tgataccaaa cagacccgtc aacgccgctg
     6481 atcccgccac cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgtcc
     6541 cgacctgcgc cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg
     6601 tgaagttatt gatacacagc ggcaccaggc ccctgtgcag tccgaacatg acgaagattt
     6661 gccagaagcc gcccattacc gcgcccgcaa atgcaggaac cgcctgataa agccagagat
     6721 aaccggcggc aatcatttcg cttatccagg ttgatagctg ccccaccagc agaaaggtga
     6781 cgggtgtgat aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta
     6841 accacgcatt aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa
     6901 taaccgatga cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccggcg
     6961 catccgcttt ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccac
     7021 caatcaccat ggcagtaaat ggattaccgc cgaagcgttt gcccgcggtg tatcccagga
     7081 ttatcgggaa gaacccaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac
     7141 tttgttcggt cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg
     7201 ttgccgccat cagaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt
     7261 ttagcagatt acctttctca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg
     7321 ccacgctgtt aaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact
     7381 gaccaccgct ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg
     7441 ctttgctttc atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa
     7501 tgttatctgc gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc
     7561 ctctaccgct ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat
     7621 caagcaattt ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata
     7681 atatttcagt gttctttgcg cacgcgctct atatttatgg ctaaaaacat aatctctgcg
     7741 ggtgaaattt tacgttgata ctgcaaacca ataaaaatgg cgatccgttc cgcacattgc
     7801 catgcttgcg ggtaattttg ttttactgct tgttgtaatg attcatcact atcgttaatt
     7861 gaagcatgtt caagaatacg ccaggataaa aacttcagat gtgtaaccag tcgctgataa
     7921 ctcaagcttt cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg
     7981 cgcattaact gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc
     8041 agatgcatgg caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca
     8101 ataatggtta acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc
     8161 cacagcaaca cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag
     8221 atataaatac tgtcctgtaa ttttcccaag cgctcctgcg ctaaagagat aagacgatca
     8281 caggttgcca tcacctcaag aggaatatga cttaagagtt cgcttaatcg cccgttcagt
     8341 tcatgactgc tcaaggcata ctctttttct attccacttg agttaattct ttcgccagcg
     8401 cgtttttgaa agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata
     8461 accaccacaa cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt
     8521 tttataaaca aaaaaaaccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc
     8581 gaatgcagta acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga
     8641 aattaattta attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg
     8701 ctcgcagtta ttaactttgt gtaattttag gaatttataa agttatatat aacaaatccc
     8761 aataattaag ttattgggat ttgtctggtg aattatttgt cgctatcttt tcccgccagc
     8821 agtttatcca gctcatcacc gcctacatga cggaaatcct gacccttcac gtagtagaag
     8881 ataaactcac aaatattctg gcagcggtcg ccgatacgtt cgattgaacg cgcacagaac
     8941 agcgcggtaa gtacgctcgg aatagtgcgc gaatcttcca tcatgtaggt catcagttga
     9001 cgaacaatac cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct
     9061 tcgtcaatgt ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga
     9121 cggcccagcg actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc
     9181 gcagtacggc agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta
     9241 ctgatcacca taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc
     9301 acgcaggctt catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg
     9361 cgcttcgcca gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc
     9421 tccaccatgc cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac
     9481 tggccggaaa tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt
     9541 aacgaccggt gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg
     9601 tgttgctgaa ctcaatcaat tcgcccaggt acataaatgc cgtgtggtcg gaacaacgcg
     9661 cagcctgctg catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga
     9721 tcagctcttc aatacgcccg gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca
     9781 gcagcacttc cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac
     9841 cagagagaga gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt
     9901 tggtcaatgc ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac
     9961 gaacgccaaa ggcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca
    10021 ccatgcccac tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc
    10081 catcaagcag aatttcacct tccgcacgct gctccgggta cagttcaaac attttgttga
    10141 aggtacgcag cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gtaacctggt
    10201 ttttggcgat atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca
    10261 aattacgaac ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct
    10321 catcgtacct gatgcttttc gctaatcagg cttaccgtag ggccgggtaa ggcgttcacg
    10381 ccgcatccgg aaaagtgtta accgtgttta ttcttcgcaa aaacaacgcg cgccagaatg
    10441 ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag ttgttgccat
    10501 tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc gatcggctgc
    10561 atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg cgcggtttca
    10621 ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac cgacgctttc
    10681 agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc cgcttcacgc
    10741 aggctgtccg gcaccagttt cagcatgttc tcagtggtgc ggataacgat cggcacctgc
    10801 aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg tgccaccacg
    10861 atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat gtcgttaatg
    10921 aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata aatccccgcc
    10981 ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc gctacccgcc
    11041 agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc agtgaacagc
    11101 gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca gatcagccag
    11161 aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt tttgaggcgg
    11221 cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg catttcaacc
    11281 atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac ttcgatgcgg
    11341 cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt gcggcaacgt
    11401 gcagaccgga ttccgcttcc gcaaattcgt tcgccagcgc agaggtgata ctattgcccg
    11461 gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag gtcaccgcca
    11521 tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca ccatttttgg
    11581 tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata ccgtaggccg
    11641 actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc gcaatgtacg
    11701 gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca aatgcggggc
    11761 cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc ggctcctgaa
    11821 agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa acgatacttg
    11881 gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag ccaggcgcaa
    11941 gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc agcgcgataa
    12001 acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg ttcggtgcat
    12061 cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc caggaggaga
    12121 tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc gccagtttta
    12181 ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagcg ggcttggttg
    12241 cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa tgccggatgc
    12301 ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac cgctactgtc
    12361 tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg gcaggctggc
    12421 gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga agaatttcag
    12481 cacctctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga acgtggtaga
    12541 ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga aggttttgct
    12601 ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa ccggtttacc
    12661 atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag catattcaac
    12721 ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt tacctttacc
    12781 gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt tccactcttc
    12841 gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat ctgcgcggcg
    12901 tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg cgatggcttc
    12961 atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac catccagcac
    13021 cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc caatcacggt
    13081 cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg cgtcagaggc
    13141 accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac cgataccctg
    13201 gtagttaact ttattaccgg tttctttctg gtaagtgtca gcccatttgg catacaccgg
    13261 cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag agaaagcact
    13321 catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt tcataatgtc
    13381 tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata ggacaaacag
    13441 gtgacagtta tatataagga atatgacatt tttatgacag aaaggaatta ccaaataact
    13501 tactgatttt aaatgtgaag atgaaaatcc cacatgccat ctttttgtca tgatgcattt
    13561 aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt tgtcagacct
    13621 attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc ggggctgggc
    13681 gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat ttttcaccat
    13741 acactgttga tttatccatg tctgttggca actcaccacc gttcactttg atttccttat
    13801 tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc attgaattgt
    13861 ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc agtatcccca
    13921 ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc gcacttccaa
    13981 caacagatga aaaatctgca gctggtattt ctttaaaatc aaaacaaact ggctttaaat
    14041 tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca tcagccatcg
    14101 gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc actatctttg
    14161 tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat actatcgctt
    14221 tagccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt gtattaccag
    14281 acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca agaatatcta
    14341 tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta gagtgaaccc
    14401 cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg aattctccta
    14461 cgttaccagt atcacaatga cagcgtccaa gatatgagga tccattagtt acagtggtgt
    14521 taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatccc
//
LOCUS       DAJTOW010000095        14465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTOW010000095 DAJTOW010000000
VERSION     DAJTOW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.095"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(337..582)
                     /locus_tag="OO244_003091"
     CDS             complement(337..582)
                     /locus_tag="OO244_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664826.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OO244_003092"
     tRNA            complement(1227..1302)
                     /locus_tag="OO244_003092"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OO244_003093"
     CDS             complement(1408..2115)
                     /locus_tag="OO244_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5664827.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OO244_003094"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OO244_003094"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5664828.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO244_003095"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO244_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5664829.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO244_003096"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO244_003096"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5664830.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OO244_003097"
     CDS             complement(7299..7574)
                     /locus_tag="OO244_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5664831.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO244_003098"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO244_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5664832.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OO244_003099"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OO244_003099"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5664833.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OO244_003100"
     CDS             9534..9860
                     /locus_tag="OO244_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5664834.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OO244_003101"
     CDS             10044..10763
                     /locus_tag="OO244_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5664835.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OO244_003102"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OO244_003102"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5664836.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13064
                     /locus_tag="OO244_003103"
     CDS             12102..13064
                     /locus_tag="OO244_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5664837.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13165..>14465)
                     /locus_tag="OO244_003104"
     CDS             complement(13165..>14465)
                     /locus_tag="OO244_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5664838.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3792 a         3471 c         3718 g         3484 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    13861 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atcca
//
LOCUS       DAJTOW010000096        14314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTOW010000096 DAJTOW010000000
VERSION     DAJTOW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.096"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..3047
                     /locus_tag="OO244_003105"
                     /pseudo
     CDS             1..3047
                     /locus_tag="OO244_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3057..6703
                     /locus_tag="OO244_003106"
                     /pseudo
     CDS             3057..6703
                     /locus_tag="OO244_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6764..7171
                     /locus_tag="OO244_003107"
     CDS             6764..7171
                     /locus_tag="OO244_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664839.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7713..8801
                     /locus_tag="OO244_003108"
     CDS             7713..8801
                     /locus_tag="OO244_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5664840.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8812..11091
                     /locus_tag="OO244_003109"
                     /pseudo
     CDS             8812..11091
                     /locus_tag="OO244_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11084..12220
                     /locus_tag="OO244_003110"
     CDS             11084..12220
                     /locus_tag="OO244_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5664841.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12217..14220
                     /locus_tag="OO244_003111"
     CDS             12217..14220
                     /locus_tag="OO244_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664842.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3550 a         3755 c         3648 g         3361 t
ORIGIN      
        1 ttatgcgaat aatgtcatta ttattctgcc gtctggcatt gcacctgact acttggagca
       61 atattccaga tttgaattac gcctacgtaa acatttatca ctggcggaag agtctctctg
      121 gcaaaaatgt gcacaaaaac ttattgccgc aattccacat attccagaat ggcggcaacc
      181 attaattgct ttgttattac ccgaaaaacc agaaattgca catgaaattg cccagcatct
      241 actggggcaa aaaaaattac cctcgcttga gtggttaaaa atagtggcga ctgatgagca
      301 cattcttgcc tcattagaaa aatatcacga gccatatgcc atttttgatg attactattg
      361 tggtgcgata tggtcagcca ccgtattaca ggagcaaggt gttgcagccc tgccccgatt
      421 tgctccctat gccgcaagtg actactgcgc cgatgtgttg cgtcatatca atcatccgtt
      481 cgcattaaca ctgcttatac gtgtagccgg gcatactaaa cgctgtcacg atcgaatgac
      541 gaaagcctgc gctgcgttcc cacacgcagc actggcagca ctggcggaac ttctagtgca
      601 aaaagaagag aatagttggc gcattatgct aatgacgatg tttatctcgc aaccagcact
      661 ggcagaacag gtcattccct ggctttcgac acccgcagtt gcagtgctga aatcatgcca
      721 gcaccaactg acacagccct caaaccatgc cagcgccgat ctactgccag ccatagtagt
      781 ctccccacca tggatttcga aaaagaaaaa atcgccgatt ccggtgctgg atttagcgcc
      841 attaggcatc gagccaatct gttatctgac agaagaaatc agtaatcaac ttttggcgaa
      901 atatatctgg tattcaaaac acatcacggt tagccatgaa gaaagtacta ccaacctgtt
      961 ggcaaggatg ggttttcaac gacggatcgc tggtacatat attaaagctc ccgaagcggt
     1021 agttgaggca tggctaaatg aagattattc aaccttacta agtgaattta aggtgtttca
     1081 ttcacctacc gggcattatt ggcagttggg gattttgaca acattgccgc tggaggctgg
     1141 agaaagcagt aaaagcatgg aatgccctta ctctatctcc acataccgat accgaatacg
     1201 ccatgttaca ttttggactc aaaggtttac ctggtttagt taactcaatt acacgctatc
     1261 cacaggaagc cttgcccatc acgaattact tcgcagcgag tgagctggct cctgccgtcg
     1321 cccgtgcctt caacaaactc aaaacgctac gcgaaaacgc ccgcacctgg ctgttgaaat
     1381 acccggaaca tgcccttacc ggcctgctgc ctgcggcgct cggcaaagcc ggtgaagcac
     1441 aggataacgc ccgcgctgcc ttgcgtatgc ttaccgaaaa cggtcatcag ccattactgc
     1501 aagaaatcgc ccggcgttat aaccagtcgg aagtaacaga cgcggtgaac gctctgcttg
     1561 cgctcgatcc cttagataat cacccgacaa aaatccccac tcttccgacc ttttatcagc
     1621 catcgatctg gacgcgcccg gtattaaaag caaacgccca atcactgcca gataacgccc
     1681 tcctccacct cggcgaaatg ctccgcttcc ctcaggaaga ggctctgtat ccgggattat
     1741 tgcaggtgaa agacgcctgt accgctgact ctctggctga atttgcctgg gatctgttta
     1801 ccgcctggca gaccgctggc gcgccgtcga aagagagttg ggcgttcact gcgttaggcg
     1861 ttctcggtaa cgatgacacc gcccgcaaac tgacgccatt aatacgcgcc tggcctggtg
     1921 aatcccagca taaacgcgcc accgttgggt tggatattct cgccgctatc ggtagtgata
     1981 tcgccctgat gcagcttaac ggcattgctc agaaactgaa attcaaagca ttacaggagc
     2041 gggcaaaaga aaagattgcc gacattgccg agagccgcga actcacggta gcggagcttg
     2101 aagatcggtt agcaccggat ctcggtctgg atgataacgg ttcgttgctg ctggattttg
     2161 gcccacgcca gttcaccgtc agctttgatg aaaccttaaa accgtttgtg cgtgatgttt
     2221 ccggcagccg cctgaaagac ctgccaaaac caaacaaaag cgattatgag tcacaggcga
     2281 acgatgctgt taaccgctac aaattgctga aaaaagatgc gcgtaccgtc gcggcccagc
     2341 aggtagcaag gcttgaatcc gccatgtgcc tgcgtcgccg ctggtcgccg gaaaacttcc
     2401 aactcttcct ggttgtgcat ccgctggttc gccacttaac ccgccgtctg atttggggcg
     2461 tttatagcgc cgacaaccag ctactggcct gtttccgggt agcggaagat aacagctaca
     2521 gcaccgctga cgatgatctt ttcaccctgc cggaaggcga tatctctatc ggcattcctc
     2581 acgttctgga aatatcaccg acggatgctg tcgcctttgg tcagcttttt gccgactacg
     2641 aactgctacc accgttccgt cagcttgacc gtaacagtta cgccctgaca gaagccgagc
     2701 gcaacgccag cgaactgacc cgctgggcag gcagaaaatg cccgagcggc cgggtcatgg
     2761 ggctggcaaa taaaggctgg ataaagggcg aaccacagga tggaggctgg atgatcaaac
     2821 ctctcggtcg ctggtcgtta atcatggaaa tcgacgaagg ttttgcggtg ggtatatcac
     2881 cagccgaact tagcgctgag cagctcttaa gcaagctgtg gctatgggaa ggcaaagcgg
     2941 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     3001 tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     3061 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     3121 aaacaccact cagtcatcgc aattggccgg gcgaaccagt gcctgttatc actgaaagca
     3181 tcattcagac actgggtgat gaattgctac aaaaagctaa gaagaaaaaa aacattgtct
     3241 ggcgttatga aaatttttca ccggagtggc agtccgccat tacgcaggcc atcaacttga
     3301 tcggcgaaca caaaccctca gtcccggccc gcacaatggc ggcactagcc tttatcgcgc
     3361 aaaatgacaa ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga
     3421 ctgaggtggt gattgcacgc cagtttattg cacggtgtta tgagagtgat cctctggtag
     3481 tcacattgca gtatcagaac gaggactatg gctatggtta tcgctcagaa acctataacg
     3541 aattcgatct ccgactgcgt aaacacctct ctctggcaga ggaaagctgc tggcagcgtt
     3601 gcgccgacaa actcattgcc gcactgccag gaataaccaa agttcgccgc ccttttattg
     3661 cgctgatcct ccccgaaaaa ccagaaatcg ccaatgagtt ggtaagcctt gaatgcccgc
     3721 ggactcattt tcattctaag gagtggttaa aagttgttgc tactgacccc aaagcggtga
     3781 gaaaactcga acgctactgg agccaggata tatttagcga tcgagaagcc agctacatgt
     3841 cgcatgaaaa ccacttcggc tacgcggcct gcgctgccct tttgcgcgaa caaggactgg
     3901 cagtcgttcc gcgcctcgcg atatatgccc ataaagaaga ttgcggcagt ctgctggtac
     3961 aaattaacca tccgcaagtc atccgcacct tgctgttagt tgctgataaa aacaaaccca
     4021 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca tgcgacactc gccgcactgg
     4081 cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc atcgaagaca
     4141 aaaagctgcc tgcacagcaa aaagctcgag atgaatactg gcgtacgctg ttacaaacgc
     4201 tgatgacatc gcagccacaa ctggcagcag aagtgatgcc gtggttaagt actcaagccc
     4261 gggcagtgct gaatagttat ttatcggcac cgcccaaacc agttattgat agcaccgata
     4321 acagccaaat gcctgaaatc ctcgtttcac caccatggcg tgggagaaag aaaaagactt
     4381 ccatacgtca cttttcgctg cctgatctta cgcttgcacc gcaaacgtac aggtcgccga
     4441 ataaacactg gacgccggaa cagcaaactg caattgacca ttttagcgcc atgtcttttg
     4501 acgaacgtct tgctacgaga ggagcggata tcttcttgcg ggaattaggt tttgacgttc
     4561 atagcctgga atttaataag cgtcttctta atggacagtt ggatgccttt gatgctcagc
     4621 aactgcaaga atacacatca ataggactat tgcgtactta tcacgcgcat caggccaaat
     4681 ctaaacagta ccgggaagac gccaccaatg cattactggc acaagatagc attgcgttga
     4741 tgggagggtg gcgaactttt cagcacggat tttactatgg taatgaacag cgttggggga
     4801 tttggaatct gtaccttatc gcgcagatgc cgcgcgaaat ggcggtatcg tgctggcaac
     4861 aaattgtcga ggctgatttt cattacaccg gggtggaata tttgttaagt gtgctgggta
     4921 ccgatgcttt gcctggatta aacaccgcct ttgcccgaca tccaaaagag atatttccgc
     4981 tgctgattca tttcggcgca acagaactgg ccctgcccat tgcccgcgtc tggcaccttt
     5041 ttgcgggcca gcgtaatctg gctcgccagt ggattttaca atggccggaa catacggcta
     5101 ctgcacttat ccctcttgtc ttcaccaaat ctagcgataa aagcgaagct gcattacttg
     5161 ccctgcgttt actttacgaa catgggcatg gcgaatggct acaaactgta gccaatcgtt
     5221 ggcagcgtac agatctatgg cctgccctgg aacaattact taaacagagt ccaatagaga
     5281 tttacccggc acgcattcca aaagcccctg atttctggca tccgcaaatg tggtccaggc
     5341 cgcgccttat cactaataat caacctgtta ccgatgacgc tctggaaatt atcggcgaaa
     5401 tgctgcgctt tacccagggg ggacgttttt atagcgggct ggaacaactg aaaacgttct
     5461 gccagccaca aacgctggca gcttttgcct gggatctctt cactgcgtgg caacaagctg
     5521 gtgcccccgc aaaagacaac tgggcatttc tggcgttaag tctctttggt gacgaaagca
     5581 cggcacggga tctaacgaca catatcctcg cctggccaca agaaggcaaa tctgcccgtg
     5641 cggtcagtgg catgaacatc cttaccctga tgaataatga tatggcgctg atgcagctgc
     5701 atcatatatc gcaacgggcg aaatcccgcc ccttacgtga taacgcggcg gaatttcttc
     5761 aggtagtcgc agaaaatcgc gggctaagcc aggaagagtt agcggacaga ttagtcccaa
     5821 cactgggcct taatgatccg caggcgttga gttttgattt tggtccccgg cagtttaccg
     5881 ttcgcttcga tgaaaacctc aacccggtta tctttgatca gcaaaacgtt cgccagaaaa
     5941 gtgttccccg gttgcgcgcc gatgacgatc aactgaaagc gcccgaggca ctggcccgac
     6001 taaaagggtt aaaaaaagat gctactcagg tgagcaaaaa cctgctcccg cgtcttgaag
     6061 ctgccctacg taccacccga cgctggtcgc tggcagattt tcattctctg tttgttaatc
     6121 atccctttac ccgtctggtt acccagcgat taatatgggg ggttatccgg caaatgaacc
     6181 gcgtcgttta ctcaacgcct ttcgtgtggc cgcagagggg gagttctgca atgcgcaaga
     6241 tgagccaatt gacctgcctg cggacgctct gattggcatt gcccacccgt tagaaatgac
     6301 agcagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta tgccgccttt
     6361 tcgccagttg tcgcgccgca cggtgctgct cacacctgac gagtcaacca gtaacagcct
     6421 gactcgctgg gaaggtaaat ccgctaccgt tgggcaactt atgggaatgc gatacaaagg
     6481 ctgggagtca ggttatgagg acgcatttgt ctatgacctg ggtgagtacc ggctggtcct
     6541 taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa tgagcttccg
     6601 ttctcttcga gtgtaccgtg acaataaatc cgtcactttt gccgaacttg atgtgtttga
     6661 tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaacaaag cgggcagtga
     6721 tgcgactgcc cgttatctac acgacttacc agcggggaaa gcgatgatcg tgcaaaaaga
     6781 gctggttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt ccttcagact
     6841 tgagatccat aaatgctctg aattatttac aggttccgtc taccgactgg aacggttccg
     6901 gctacgtcca acatttcatc aacgtgatcg agaggatgtt gacccgctaa taaatgatgc
     6961 gttgatttat ataagagatg agtttattga tgagcaaaaa ttacgaggcg aatcacctga
     7021 acctgtaata gtaattttta attgtaaact acagaatata ttcaaccaac aaatagaatt
     7081 atatactcta gataattcaa gtcgcagcac atgcaacttg aagtatgacg agtatagctt
     7141 tattaaaggg gggaacggta ttccccctta gatgcgtttc aatttgcccg ttacttcaac
     7201 aaatcaatca accgtaacag agccactaat agctccagta cagcaatgag cacggttaac
     7261 ttttgtgtca gcgtgtccat ccgctctcct tattaaggag cacaacacca gcaccttacc
     7321 cttacactgc ctgtcgatac tggtttacgt ttgttgagtc gccatgttgc actccggatc
     7381 agagcactac ctgcggcacc acccgtccgg tagccaggac ttgaaatagg ttcgatccgg
     7441 cgcaacttcc caacagtcag gattataaat ctcattatct taattgcaac actgtccaga
     7501 ggtggttata attcgttcgt tggaacacga tgttgcacag gcagtggtgt taggcctgaa
     7561 aatagtaaat aaaagaacct gcctcaccag cagttttttt tatttactgt gatctgcttt
     7621 ccagatattt ttcgctcaaa caactaatgc gccaaacatt tattgcgcgt aaaatatcgt
     7681 ttatttcatt aatacatttc agggatgaat atatgtcacc gcagaataat catctgcagc
     7741 gtccgcctgc tgctgtgtta tacgccgatg aactggcaaa attaaaacaa aatgataacg
     7801 caccttgccc gcccggttgg cagttaagtt tgcctgcggc ccgtgctttt atccttggcg
     7861 atagcgcgca aaatattagc cgtaaagtgg tgattagtcc ctccgctgtc gaacgtatgt
     7921 tagtcactct tgccaccgga cgcggtttga tgttggtggg ggaacctggc accgcaaaat
     7981 ctctcctgtc tgaattactg gcaacctcta ttagcggcga tgccgggtta accatccagg
     8041 gcggcgcatc tactaccgaa gatcaaatca agtacggctg gaactacgcc ctgcttatca
     8101 atcatggtcc atcaacggaa gcgctggtcc ctgccccgct ttaccaggga atgcgcgacg
     8161 gcaaaatcgt ccgttttgaa gagataacgc gtacaccgct ggaagtgcaa gactgtctgt
     8221 taggtatgct ttctgacagg gtgatgacgg tgccggaact cactggtgaa gccagtcagc
     8281 tctatgcgcg agaaggcttt aatattattg ccactgccaa tacccgcgat cgcggcgtca
     8341 atgaaatgag tgctgcgcta aagcgtcgct tcgattttga aactgtcttt ccgattatgg
     8401 atttcgccca ggagctggag cttgtcgcca gtgcttcggc gcgtttgctg gcccatagcg
     8461 gtattccgca taaagtgcct gacgcggtac tggagttact ggtccgcacc ttccgcgatc
     8521 tgcgtgccaa cggcgaaaag aaaacgtcaa tggatacact aacagcgatt atgtccaccg
     8581 ctgaagccgt gaacgtggcc cacgccgtag gcgtccgcgc ctggttttta gcgaatcgcg
     8641 caggtgagcc agccgacctg gtggactgca ttgccggaac catcgtcaaa gataacgaag
     8701 aagatcgcgc tcgtctgcgc cgttacttcg aacagcgtgt cgccacacat aaagaagctc
     8761 actggcaggc ttattatcaa gcccgccacc gcctgccgtg aggaaagatg catgagcgag
     8821 ccgttaattg tcggcatccg gcatcatagt ccggcctgcg cccggctggt gaaatcgtta
     8881 atcgaaagcc agcggccacg atacgtgttg attgaaggcc ctgctgattt taatgaccgg
     8941 gtagacgaac tgtttttagc ccaccagctt ccggtagcta tttacagtta ttgccagtat
     9001 caggacggtg cagcccccgg gcgtggtgcc tggacgccat ttgctgaatt ttcgccggag
     9061 tggcaggcgc tacaagccga tcgtcgtatt caggcacaaa cttacttcat cgatttgcct
     9121 tgctgggcgc agagtgaaga agaggacgat tcgcctgata cgcaagagga aagccagacc
     9181 ttgctgctgc gtgccacccg catggataac agcgacaccc tgtgggatca cttgttcgaa
     9241 gatgaaagcc agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg
     9301 ggcgactccc ccggcgatgc tctaaatcgt cagcgcgaag cctttatggc ccgctggatt
     9361 ggatgggcga tgcagcaaaa taatggcgac gtgttagttg tctgcggtgg ctggcacgct
     9421 ccggcactgg caaaaatgtg gcgcgaatgt ccgcaggtaa ttaacacgcc agaattgccg
     9481 tcgctggcag atgccgttac aggttgttat ctcaccccct acagtgaaaa gcgccttgat
     9541 gtgctggcag gatacctttc aggaatgcct gccccggtct ggcaaaactg gtgctggcag
     9601 tggggcttac agcaggcggg agaacaactg ctaaaaacgg ttctcacccg tttgcgccag
     9661 cacaagttgc ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcactg
     9721 gcacagttgc gcggtcatac actaccgtta cgcactgact ggctggatgc catagcagga
     9781 tcgctgatta aagaagccct gaatgcgccg ttgccgtgga gctatcgcgg cgttattcat
     9841 cccgataccg atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt
     9901 aaacttgccc cttctacgcc acaaccgcct ctgccaaaag atgtcacttg cgaactggaa
     9961 cgtaccgcaa tctctcttcc ggcggagctt accttaaatc gctttaaccc caatggacta
    10021 gcgcaaagtc aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag
    10081 cagggaagca cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg
    10141 cttagccagc atgcggcatt gattgaggcc gcctgctttg gtgccacact cctggaagcc
    10201 gcacgccata aattagaagc cgatatgctg gacgcgggtg gaatcggcag cattaccaca
    10261 tgtcttagcc aagcggcgtc agcgggtctg gcgtccttca gtcaacaatt actggagcaa
    10321 ctcacattat taatcgccca ggaaaatcaa tttgccgaaa tgggccaagc gctggaagtg
    10381 ctatatgcct tatggcggct ggatgaaatt agcggtatgc aaggcgcgca gatattacaa
    10441 acgacgttat gcgcggctat cgatcgcacg ctgtggctgt gtgaatccaa cggcagaccg
    10501 gatgaaaagg agtttcacgc tcacttgcat agctggcaag cgttgtgtca tatcctgcgc
    10561 gatctacata gcggtgtgca actccccggc gtttcgcttt ctgcggcggt agccttgctg
    10621 gagcgacaca gtcaggcgat tcatgtcctg gcactggatc gcggcgcgac tcttggtgcg
    10681 ctgatgcgtc tggaacatcc caacgctagt gccgaagcgg cgctgacgat gctggcgcag
    10741 ttatccccgg cacaatccgg cgaggcgctg cacggtttgc tggcattagc ccgtcatcaa
    10801 ctggcctgtc agccggcatt tatcgccggt ttcagcagtc atttaaatca actaagtgat
    10861 gccgatttta ccaatgccct gcccgattta cgcgcggcaa tggcctagct accgccacga
    10921 gaacgcggga cgctggcgca tcaggtgctt gagcattatc aactggcgca acttcccgtt
    10981 tcggcactgc aaatgccgtt gcattgtcca ccgcaagcca ttgcacatca tcaacaactc
    11041 gaacagcagg cactggcatc gctgcaacac tggggagttt tccatgtctg aactgaacga
    11101 tcttctgacc acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac
    11161 gacgctttgt gggctggatg acaacgcccg gcagatagat cacgcgctgg agtggctgta
    11221 tgggcgcgat cctgaacggc tccagcgtgg tgaacgctct agtggattag gtggctcaaa
    11281 tctcaccact cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga
    11341 gcggctggaa agcgatgccg tactgcgcta cggcattgaa gatgtggtga caaatctcga
    11401 cgtgctggaa cgtatgcagc cttccgaaag cctgctacgc gccgttttgc acaccaaaca
    11461 tctgatgaac cccgaagtac tggctgccgc ccgcaggatt gtgcgccagg ttgtggaaga
    11521 aattatggcg cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgtcg
    11581 tcgccgctca tttattccac tggcgcgaaa ctttgatttc aaaagtactc tgcgcgccaa
    11641 cctgcaacac tggcacccgc aacacggcaa gttgtatatc gaattccccc gctttaacag
    11701 ccgcattaaa cgccaaagcg agcaatggca actggtctta ctggttgatc aaagcggatc
    11761 gatggtcgat tcggtgatcc actctgcggt gatggcggcc tgtttatggc agttacccgg
    11821 cattcgtacc catctggtgg cgtttgacac aagcgtcgtt gatctcacgg cagacgttgc
    11881 cgatccggta gagttattaa tgaaagtgca attaggcggc ggaaccaata tcgccagtgc
    11941 catggagtat ggtcggcaac ttattgaaca acctgcaaaa agcgtcatta tcctcgtgag
    12001 cgatttttat gaagggggtt catcatcatt gctgacgcac caagtgaaaa agtgtgtcca
    12061 gagcggcatc aaagtgctgg gactggcagc gctcgatagc acagcaacac cttgctatga
    12121 ccgcgatacg gcccaggcgc tggttaatgt cggcgcacaa atagccgcca tgacaccggg
    12181 cgagctggca tcatggcttg cggagaatct tcagtcatga attcactacg tccggaatta
    12241 ttagaactga caccgcaggc actgacggcg ttaagcaatg ccggttttgt taagcgcagc
    12301 cttaaggaac tggaaaatgg caacgtcccg gaaatcagcc atgagaacag cgctttaatc
    12361 gccaccttca gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct
    12421 cagtgcacct atggggccag cggcatgtgc cgtcatcgcg tgatgctggt gttaagttat
    12481 caacgacttt gtgctaccgc ccagcctacg ggaaaagaag aggaatggga tccggcaatc
    12541 tggctggaag aactggctac ccttcccgat gccacccgca aacgcgcaca ggcgctggtc
    12601 gctaaaagca tcaccattga gttgttctgt gcgccgggtg aaattccctc tgcccgctta
    12661 ccgatgagcg atgtgcgttt ttattcccgc agcagtattc gtttcgcccg ttgtgattgt
    12721 attgaaggca cactttgcga acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa
    12781 gcgcagcaag cggaattgac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca
    12841 tctgacgatc cgtttgccag cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta
    12901 agccaggcat tatggctgag cggcatcagc cagccgctta ttcactacga ggccgctttc
    12961 agccgcgcgc agcaagcagc ggaacgctgc agctggcgat gggtgagtga atcgctacgg
    13021 caactgcgcg ccagcgttga tgccttccac gcccgcgcca gccactatca tgccggagaa
    13081 tgcttacgtc agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg
    13141 agcgacagtg ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt
    13201 gccggagaag caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag
    13261 gatattgagc attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg
    13321 cacctttcgc gcagttggcc gcgaagtgaa caggaagact caccggcagc tacgcgtcgg
    13381 ctgtttagtt ttcaggctgg cgcactggcg ggtgggcaaa ttgtttcaca agcagcaaaa
    13441 cgcagtgccg atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtaccg
    13501 ctgtcgcctg atgcctggca aatgttgagc gcgccgttac gccagccggg cattgtggct
    13561 ttgcgggaat atttacgcca gcgtcccccc gcctgcatac ggcctcttaa tcaggtcgat
    13621 aacttattta ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag
    13681 acgctggatg cgcaggtaat cagcggcgaa ggtgaagata atctgctgac gttatcactg
    13741 ccagcctcag ccagcgcgcc ttatgccatt gaacgcatgg cggcgctttt gcaacaaaca
    13801 gacgaccccg tgtgtctggt ttctggcttt gtcagttttg ttgatgggca attgacactg
    13861 gaaccacagg taatgatgac aaagactcgt gcctgggcgc tggacgcaga aactgcgcct
    13921 gtggtggtat cactaccttc tgccagcgtt ctgcctgtgc cgtctactgc tcatcagttg
    13981 ctgatgcgct gccaggcgtt acttattcaa ctgctccata acggctggcg ctatcaggaa
    14041 cagagcgcta ttaatcaggc agagttgctg gcgaatgacc tcaccgcggt gggtttttat
    14101 cggctggcac atgtgttggc acaatttcgt aatacagaaa gcgaggcacg ggtagaagca
    14161 atgaataacg gtgttttgct ttgcgaacaa ttattcccca tgcttcagca acaaggatga
    14221 aatagtgctt tttactaaga gttctactcc agttccggac tgctcacgcc acggtattag
    14281 gcatatccta tatagcccct ggtgagagtc acca
//
LOCUS       DAJTOW010000097        13964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTOW010000097 DAJTOW010000000
VERSION     DAJTOW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.097"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO244_003112"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO244_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5664843.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO244_003113"
     CDS             2219..2767
                     /locus_tag="OO244_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5664844.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO244_003114"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO244_003114"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5664845.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO244_003115"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO244_003115"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5664846.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO244_003116"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO244_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5664847.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO244_003117"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO244_003117"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5664848.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO244_003118"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO244_003118"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5664849.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO244_003119"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO244_003119"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5664850.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO244_003120"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO244_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5664851.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO244_003121"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO244_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5664852.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3562 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgcagca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gtgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagtg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTOW010000098        13797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTOW010000098 DAJTOW010000000
VERSION     DAJTOW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.098"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO244_003122"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO244_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5664853.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO244_003123"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO244_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5664854.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO244_003124"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO244_003124"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5664855.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO244_003125"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO244_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO244_003126"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO244_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5664856.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO244_003127"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO244_003127"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5664857.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO244_003128"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO244_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5664858.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO244_003129"
     CDS             7489..7638
                     /locus_tag="OO244_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664859.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO244_003130"
     CDS             7839..9422
                     /locus_tag="OO244_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5664860.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO244_003131"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO244_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5664861.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO244_003132"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO244_003132"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5664862.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO244_003133"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO244_003133"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5664863.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..>13797
                     /locus_tag="OO244_003134"
     CDS             13381..>13797
                     /locus_tag="OO244_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5664864.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNLLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3191 a         3640 c         3495 g         3471 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgag
    13681 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataacct cttgcaaaat
    13741 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttgggg
//
LOCUS       DAJTOW010000099        13776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTOW010000099 DAJTOW010000000
VERSION     DAJTOW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.099"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            166..780
                     /locus_tag="OO244_003135"
     CDS             166..780
                     /locus_tag="OO244_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCR5664865.1"
                     /translation="MMAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSK
                     EREKAEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPC
                     ISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRI
                     GQEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            777..983
                     /locus_tag="OO244_003136"
     CDS             777..983
                     /locus_tag="OO244_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HCR5664866.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            961..1632
                     /locus_tag="OO244_003137"
     CDS             961..1632
                     /locus_tag="OO244_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR5664867.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTQKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSECGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGAWA"
     gene            1623..2141
                     /locus_tag="OO244_003138"
     CDS             1623..2141
                     /locus_tag="OO244_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5664868.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKLM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKNLDMQLAI"
     gene            2738..3061
                     /locus_tag="OO244_003139"
     CDS             2738..3061
                     /locus_tag="OO244_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5664869.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            3045..3521
                     /locus_tag="OO244_003140"
     CDS             3045..3521
                     /locus_tag="OO244_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5664870.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGMVREIEV"
     gene            3518..3955
                     /locus_tag="OO244_003141"
     CDS             3518..3955
                     /locus_tag="OO244_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5664871.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVSYLQEYIRTQCLK"
     gene            4561..4803
                     /locus_tag="OO244_003142"
     CDS             4561..4803
                     /locus_tag="OO244_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR5664872.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            4883..5371
                     /locus_tag="OO244_003143"
     CDS             4883..5371
                     /locus_tag="OO244_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5664873.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            5349..6845
                     /locus_tag="OO244_003144"
     CDS             5349..6845
                     /locus_tag="OO244_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5664874.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPDDGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVINAMDFGWDHPQAHIQLW
                     WDKDEDVIYLSRVWKAKQKKATEAWSAVKAWSKNTPTAWPHDGHQHEKGGGAQLKEQY
                     ADAGFDMLPDHATWPDGGNAVEPGIAEIRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDILSAVRYGYMMRRFAIQMRDIKDPKEIDYSSYNIPCGVG"
     gene            6845..9046
                     /locus_tag="OO244_003145"
     CDS             6845..9046
                     /locus_tag="OO244_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5664875.1"
                     /translation="MADDRKMADWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGA
                     QWEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEA
                     LAEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISL
                     LPVYDPATCVFFDQNSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLFRDDTGTQ
                     YDWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELI
                     GERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAM
                     DAQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLK
                     NKNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVD
                     SIFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTG
                     EVVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDL
                     VSSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPEPAMV
                     AAQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIRE
                     ALKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQKQPQQ"
     gene            9137..10030
                     /locus_tag="OO244_003146"
     CDS             9137..10030
                     /locus_tag="OO244_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5664876.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            10049..11302
                     /locus_tag="OO244_003147"
     CDS             10049..11302
                     /locus_tag="OO244_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5664877.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSSKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGTATSLTLAAEPAHKVEAKDANDMPVDNRQGTITVSASGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYVNVDANAANGAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            11344..11532
                     /locus_tag="OO244_003148"
     CDS             11344..11532
                     /locus_tag="OO244_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664878.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKHKVAKAEEDGENEG"
     gene            11513..11974
                     /locus_tag="OO244_003149"
     CDS             11513..11974
                     /locus_tag="OO244_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HCR5664879.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            11984..13402
                     /locus_tag="OO244_003150"
     CDS             11984..13402
                     /locus_tag="OO244_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5664880.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQSAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEDNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            13402..>13776
                     /locus_tag="OO244_003151"
     CDS             13402..>13776
                     /locus_tag="OO244_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664881.1"
                     /translation="MADSNLNTPVIVQATRLDTSILPRNIFSQSYLLYVIAQGADVGN
                     VANKANEAGQGAYDAQVRNDEQDVILVDHEIRLASAEAKIQDHETRITNAEAAIVGLD
                     SRLTTAENDIDYLTDEVVSIQNT"
BASE COUNT         3835 a         3252 c         3601 g         3088 t
ORIGIN      
        1 tgatcaggag ctggttatta ccggagacct ggcattcaga acatataagg ccagcatgga
       61 aatgaaggat ttccctaaat gtggttctga aagagaagcc gcgttaaagc tggcagagtg
      121 gatgcagaga atggctgctg caattgagaa ttactggagc gaaccataat ggctaaacca
      181 gcgcgaagac gatgtaaaaa cgatgaatgt cgggaatggt ttcaccctgc attcgctaat
      241 cagtggtggt gctctccaga gtgtggaaca aagatagcac tcgaacgacg aagcaaagaa
      301 cgcgaaaaag cggaaaaagc agcagagaag aaactacgac gagaggagca gaaacagaaa
      361 gataaactga agattcgaaa actcgcctta aagccccgca gttactggat taaacaagcc
      421 caacaagccg taaacgcctt catcagagaa agagaccgcg acttaccatg tatctcgtgc
      481 ggaacgctca cgtctgctca gtgggatgcc ggacattacc ggacaactgc tgcggcacct
      541 caactccgat ttgatgaacg caatattcac aagcaatgcg tggtgtgcaa ccagcataaa
      601 agcggaaatc tcgttccgta tcgcgtcgaa ctgattaacc ggatcgggca ggaagcagta
      661 gacgaaatcg aatcaaacca taaccgccat cgctggactg tcgaagagtg caaggcgatc
      721 aaggcagagt accaacagaa actcaaagac ctgcgaaata gcagaagtga ggccgcatga
      781 cgttctcagt aaaaaccatt ccagacatgc tcgttgaagc atacggaaac cagacagaag
      841 tagcacgcag actgaaatgt agtcgcggta cggtcagaaa atacgttgat gataaagacg
      901 ggaaaatgca cgccatcgtc aacgacgttc tcatggttca tcgcggatgg agtgaaagag
      961 atgcgctatt acgaaaaaat tgatggcagc aaataccgaa atatttgggt agttggcgat
     1021 ctgcacggat gctacacgaa cctgatgaaa aaactggaga cgattggatt cgacacccaa
     1081 aaagacctgc ttatctcggt tggcgatttg gtcgatcgtg gtacagagaa cgtcgaatgc
     1141 ctggaattaa tcacattccc ctggttcaga gctgtacgtg gaaaccatga gcaaatgatg
     1201 attgatggct tatcagagtg tggaaacgtt aatcactggc tgcttaatgg cggtggctgg
     1261 ttcttcaatc tcgattacga caaagagatt ctggctaaag ctcttgccca taaagcagat
     1321 gaacttccgt taatcattga actggtgagc aaaggtaaaa aatatgttat ctgccacgcc
     1381 gattatccct gtgacgaata cgagtttgga aagccagttg atcatcagca ggtaatctgg
     1441 aaccgcgaac gaatcagcaa ctcacaagac gggatcgtga aagaaatcaa aggcgcggac
     1501 acgttcatct ttggtcatac gccagcagtg aaaccactca agtttgccaa ccagatgtat
     1561 atcgataccg gcgcagtgtt ctgcggaaac ctcacattga ttcaggtaca gggagaaggc
     1621 gcatgggcat aagagaacta aaccttacca aagagcagca cgagtggctg aatggctggc
     1681 ttgaactgtg gggcgcatgg gtttattcag gtcgtctgga aaagcgcatg agcagcgtaa
     1741 tagcgaagct catggagagc gtagagccgg gaagagttat gacaaggcca atgtgcaatg
     1801 atgatgatgg aatgttgatt tctcaggtcg tcgattccgt catgtacatt gacaaaaaag
     1861 cctttggcat cctcctcagc tactacgctc atggttcatc taagcgagca attgcatcct
     1921 actatcacgc gactgcaaag ccacgcaaga tgtgtggacg tggtggcgag ggatggagaa
     1981 aaccttcact ggcaacctgt agaaacgaaa ttgacgacat cctgaaagcg tcattatttg
     2041 ttttgtacca accaatgcaa aatgctttca aaatgcgtaa acgtgttgag aaagttaagc
     2101 atgttgctgt taaaaacctt gacatgcaat tagccattta gccataataa gagggtaagc
     2161 tgccgttagt gactcttaag ttgcaacggt ggcttttttg tttgcacaac aggtaagagc
     2221 attgaacccg cagacctcgc ggaattggtg aaaggtgccg cgcagtgctc ttatcgttgt
     2281 ggtgaatacg caggctgatg cgttaatcag gtgaacgaga cacccgccgg tccgtgatat
     2341 ggcacaccgt gccggtcata tctgccgcgg ttaggtttac gaggatttcg taaagctggt
     2401 ctagggtgaa gccgtgaaag cggaggaagt aaaacgaggc gtcggtacac gcctatcgtc
     2461 attaagtcgg agttcagcac cgaccgccac aacccaaact gagccgtagc cactggctgt
     2521 cctgaattca tcagtgatag ttatgctgcg gccttctaca catgaccttc gtgaaagcgg
     2581 gtggaaggag gttgcgctaa caacctcctg ccgttttgcc cgtgcatatc ggtcacgaac
     2641 aaatctgatt actaaacaca gtagcctgga tttgttctat cagtaatcga ccttattcct
     2701 aattaaatag agcaaatccc cttattgggg gtaagacatg aagatgccag aaaaacatga
     2761 cctgttagcc gccattctcg cggcaaagga acaaggcatc ggggcaatcc ttgcgtttgc
     2821 aatggcgtac cttcgcggca gatataatgg cggtgcgttt acaaaaacag taatcgacgc
     2881 aacgatgtgc gccattatcg cctggttcat tcgtgacctt ctcgacttcg ccggactaag
     2941 tagcaatctc gcttatataa cgagcgtgtt tatcggctac atcggtactg actcgattgg
     3001 ttcgcttatc aaacgcttcg ctgctaaaaa agccggagta gaagatggtg gaaatcaata
     3061 atcaacgtaa ggcgttcctc gatatgctgg cgtggtcaga aggaactgat aacgggcgtc
     3121 agaaaaccag aaatcatggt tatgacgtca ttgtaggcgg agagctattc actgattact
     3181 ccgatcaccc tcgcaaactt gtcacgctaa accccaaact caaatcaaca gcggccggac
     3241 gttaccagct tctttcccgt tggtgggatg cctatcgtaa gcagcttggc ctgaaagact
     3301 tctctccgaa aagccaggac gctgttgcac tgcagcagat taaggagcgt ggcgctttac
     3361 cgatgattga tcgcggtgat atccgtcagg caatcgaccg ttgcagtaat atctgggctt
     3421 cactgccggg cgctggttat ggtcagttcg agcataaggc tgacagcctg attgcaaaat
     3481 tcaaagaagc aggcggaatg gtcagagaga ttgaggtatg agcagagtca ccgcgattat
     3541 ctccgctctg gttatctgca tcatcgtctg cctgtcgtgg gcggtcaatc attaccgtga
     3601 taacgccatc gcctataaag agcagcgcga taaggccaca tccacaatcg ctgacatgca
     3661 gaagcgtcaa cgtgatgtag cagaacttga cgccagatac acaaaggagc ttgctgatgc
     3721 taacgcgact atcgaaagtc tccgtgctga tgtttctgct ggtcgtaagc gcctgcaagt
     3781 cgccgccacc tgtgcaaagt caacgaccgg agccagcggc atgggcgatg gagaaagccc
     3841 aagacttaca gcagatgctg aactcaatta ttaccgtctc cgaagtggaa tcgacaggat
     3901 aaccgcgcag gttagctacc tgcaggaata catcaggacg caatgcctga aatgatcggg
     3961 cgatgaaaac caaaaaaaca ggagcaatac atgactaagc tttatcaccg catctcaact
     4021 tttctctctg ggtgctgggc gtttatcacg tctatttcgt tcgccatctt tagtttcggt
     4081 agcacagcat gctcgcttag tcggggtatg tggcgtgcta tttcagcact agcgccgaaa
     4141 tttttacctg aaaaggctgt ttggcgaatt gtagagcgaa tgtgtagtga gagcgttcgc
     4201 gagaagatta acgtatttgg acgtcatcct cgaaatacag gcgcattgtg cagttcgtta
     4261 ctgtagtcat tacaaagccc atcaatgggt gggcttgata atgaaaccgg aatttattct
     4321 gggcaaccag ttacggcagt accgcgaaac aacccaagcc agtaagtggg gaaataacac
     4381 tggcagccac tgaaagatga acctccagcc ttatggcaaa aaagattctt tgtggtggcg
     4441 gactgatgga aagacatcgg ttattgcaga gaccattcaa tgagtggtct cgacaatggc
     4501 ttatacccta cacgggataa cttaactgat atccctttta acggataaac ggagccaaca
     4561 atggcagaga ttattcccat gactgaagaa cagaaattcc agttagagat ttacaaactg
     4621 gtcatgaacc agaacgcagc tgcagaggaa gcatttcagt tcattggtac tgacgagctg
     4681 aagcttgagc tattcaaaat tcacttccag tcaggcggcg ctaattcaga tatcacgacc
     4741 cgcactatcg aagcggtgcg taaatcgaag gaagcgttag acctgttcac caccggagca
     4801 taaacatggc aactcaaggt ttcgacaacc catccaaatt ccgcgatgaa tgggataagc
     4861 aagcagaagg gaaataatca atatggcagc accaaagggc aaccgatttt gggaggcccg
     4921 cagtagtcat gggcgaaatc ctaaattcga atcgcctgag gcgctgtggg ctgcttgttg
     4981 tgaatacttc gagtgggctg atgataaccc gctatgggag ggtaaggtat tttcatatca
     5041 gggagaaata attaaggcta atgtccctaa gatgcgagcc atgactattt caggattgtg
     5101 caccttcctt gatatcacca ggcaaacatg gggaaccttc cggtcaatgg aaggtttttc
     5161 tgacgtcaca tcacgagcgg aagacatcat ctacgaccag aaattctctg gcgctgccgc
     5221 tgaccttctc aacgctaaca tcatcgcccg tgatttgggc ctcaaagagc agtcgcaagt
     5281 tgaagacgtg acacctgata agggagatcg cgataagcga cgctctcgta tcaaggagct
     5341 attcaaccgt ggaactggac gcgattcttg ataacctgag cgacgaagag caaatcgaat
     5401 tgctcgagct actcgaagaa gaagagaact accggaacac acacctgcta tatgaattta
     5461 cgccatacag caaacagcgt gagttcatcg acgccgggca tgactatcca gagcgatgtt
     5521 ttatggctgg taaccagctt ggtaagtcat ttactggtgc tgctgaagtc gcgtttcacc
     5581 ttacagggcg ttatccggga acaaaaggct atccggatga tggtaaatat ggcggggagt
     5641 ggaaaggtaa gcgtttctat gagcctgttg tcttctggat tggcggcgag acaaacgaga
     5701 cggtaaccaa aacgactcaa cgcatcctgt gcggtcgtat cgaagagaat gacgagccag
     5761 gctacggttc cataccgaaa gaagacatca ttagctggaa gaagtctcct ttctttccga
     5821 atcttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa gatggcattt
     5881 caatctgcta cttcaaacca tactcgcaag gccgtgctcg ctggcagggt gacacaatcc
     5941 acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt cttacccgta
     6001 ccaacaaata cggtcaattc tcaattctga cgtttacccc gctgatgggg atgtctgacg
     6061 ttgttaccaa gttcctgaag aatcccagca agtcgcagaa agtggtcaac atgaccatct
     6121 acgacgctga gcactacacc gacgagcaga aagagcaaat catcgcatcc tatcccgagc
     6181 atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga atcttccaga
     6241 taccggaaga gactattaag tgtcagccgt tcgagtgtcc tgatcacttc tacgtcatca
     6301 atgcaatgga cttcggatgg gatcacccac aggcacacat ccagctttgg tgggataaag
     6361 acgaggacgt gatttatctt tctcgcgtct ggaaggccaa acagaagaag gcgacagagg
     6421 catggagtgc tgttaaagca tggagcaaaa acacccctac ggcttggcct catgacgggc
     6481 atcagcacga aaagggaggc ggcgctcagc tcaaggaaca atacgccgac gctgggttcg
     6541 atatgttgcc agatcatgca acatggcctg atggaggtaa tgcggtcgaa cccgggatag
     6601 cagagatacg cgacatgatg ctcgacggtc gtttcaaggt atttaacacc tgcgagccat
     6661 tctttgaaga gtttcgcctg tatcaccgcg atgagaacgg gaagatcgtc aagctaaatg
     6721 acgacatcct ttctgctgtt cgctatggct acatgatgag gcgttttgca atacagatgc
     6781 gagacatcaa agatcctaaa gagattgatt actcaagcta caacatacct tgcggagttg
     6841 gatgatggct gatgatagaa agatggctga ctggcatcgc aaggtgctgt gcaactttga
     6901 taatgcctgg tcagcaacgc aggatatgcg tgagcagatt attgaggctc aacgtttcgt
     6961 ccgggtgtcc ggcgcacagt gggaaggcag cacaaacgct ggttactcat ttgatgaagg
     7021 caggtttgag cattacccgc gttttgaact gaataagatt gcccgtgaat gtgatcgcat
     7081 cattggcgag tatcgacaga atcgcatcag cgttaaattc aggccgaagg acgacaaggc
     7141 atcggaagcg ttagccgaaa agatgaacgg caaattccgc gctgactatc aggaaacatc
     7201 cggtggcgaa gcgtgtgata acgcatttga tgatgccgta acgggcggat tcggttgttt
     7261 ccgcatgtgt gccgattacg aagatgaaat ggatccgagt aacgagcagc gccgcatcag
     7321 ccttctcccg gtttacgacc cagcgacatg cgtcttcttc gatcagaaca gcaagcaata
     7381 tgaccgctct gatgctatgt gggctatgga aatgttctcc atgacgccta aagcgttcga
     7441 ggctgaatac cctgattcca tcgcggcaag cctttttcgt gatgacactg gcactcaata
     7501 tgactggtca acgccagatg ccatctatgt tggacgctac tacgaagttc gcatagagaa
     7561 ggtgaagctc acagcatggc gtaaccctgt cagcggagaa acggcaatct atgatgaaga
     7621 gcaaatcaaa gatattgtcg acgagctgac cgatggtgca ttcgaactga ttggtgagcg
     7681 gacagtgaag aaacgccgag tttattgcgg tcttctgtct ggcgctgaat ggctggaaga
     7741 accgaagcgt attccgggcg aacatattcc tctcatcccg gtatatgggc gtcgttcatt
     7801 tgttgataat caggagcgaa tcgaaggcca cgcagcaaaa gcgatggatg cacagcgtct
     7861 tgagaacctg atggtttcca tgattgcaga taacgccact caggctggcg gtgatggcat
     7921 tcctgtagtt gatgttgaca tgattcctgg tcctctcgcc actcattggg cggagcgcaa
     7981 caaaaagcgc ccggcgttcc tgccgatggt cagtctgaaa aacaaaaacg gagatattac
     8041 tgcgcaggct caggtcagca gttatacgcc tccgacacaa atgcctccag ctcttgccgg
     8101 gctattgcag tacaccggaa cggctattca gcaaattaca ggtgcgtcgc agcttgagaa
     8161 catgccgagc aacgtcgcca ccgataccgt tgatagcatc tttaaccgga tggatacgca
     8221 gtcctatatc tacatggaca acatggctaa atccatgcgc cgtgctggcg tcgtgtggct
     8281 ttctatggct cgtgaagtct atggcagcga tacgccaatg cgcatcgtta atgaggatgg
     8341 cagcgatgac gtggcgctga tgactggtga agtggttgac cgtcagacag ggcaggttat
     8401 cgcgcttaac gacctttcgc agggtaacta tgaagtgact gtcgatgtcg gtcagtcgtt
     8461 cgctactcgc cgtgatgcaa cggttaagtc gttactttcc atgctggcac ttatcccgcc
     8521 aggaacgccg aagcatgacc ttgtatcgtc gatgattctc gacaatatgg acggcgaagg
     8581 gatggacgac cttaaagaat acaaccgcaa tcagttgctt ctgtctggcg ttatcaagcc
     8641 gagaacgcct gaagaacagc agatggttga acaggcgaaa caacaacagg ccagtcagcc
     8701 agaaccggct atggttgcag cgcaaggtca gcttcttgct ggtcaggctg aattgcagaa
     8761 agctcagaac gaacaggcag ccattcaggt taaagcattc caggcacaga ctgatgctca
     8821 ggttgcagcg gcaaatgttg tgaaaatcct cgcatctgcc gatagccagc agaaatctga
     8881 tatccgcgag gctctgaaac tgctcggaca gttccagcaa cagcaaggag acaatgcccg
     8941 tgctgatgca gagcttgtcc tgaaaagtca ggcacagggc catgcgcagc gcatggacat
     9001 cagcagcatc ctgcaaaaat caactcagaa acaaccacag cagtaattaa cccataacgt
     9061 gcaatggctg tctttatgag gcctggcaac ctattgcctt ccgatgggct gaacatcgag
     9121 taaacagggg taacaaatgg accagatggc agaaaacaca ccagaagttg aaatcgaaac
     9181 cgacgcgtca gagcagattc ctgatgatgt cgaactggct gaagaagtcg aaacagaaga
     9241 tggcagtgag tcctccggca atgatgcaga ggaagctact gaaactgatg acgacgaatc
     9301 agaacaggaa ttctactttg gtgacgaaaa gctggattcg ccaaccagcg aagatggcgc
     9361 agagcatgga ctggtaaaac acctgcgcaa gacgattaaa gagaaagacc gcgagctgaa
     9421 agagctgatg cgtcagtctc agaaacccgt cgagcagcag ccggtaatca ctcaaccacc
     9481 gcgaatgcca aaactggatg atgaggacat cgggttcgat gaagaaatct accagcaacg
     9541 catggctaag tgggcagagg ataacggcaa gtaccagcaa caggagatgg ctcgcaagca
     9601 gaaggagcag gagcttcagg ctgcctatca agagcgatta tccaaatatc agcaacgtgt
     9661 taaggctctc aaagttcctg gctatcagga agctgagcag gccgtactcg aggaaatccc
     9721 catcgagaca caaaacgcga tcctgtttga gtcagagaag ccggaaatcg ttgttctggc
     9781 actcggtcgc aacgctgaac tgcgcaagca actggcagaa gctaccaacc ccgtagcaat
     9841 tggtcgtctg ctggaacgta tcgaatcgaa ggccagaatc atgccaaaag caaaaaccac
     9901 ggcagccaca accccgacag ttaaggggag caacggcgca gtaatcaata acctcgacaa
     9961 actgaaagcc aaggcgctgg aaactggtga ctggacgccg tatttcgccg ctaaaaaggc
    10021 aaaaaaataa cctatcggag cattaagcat ggctaaccaa ttagcaaaag accttgaaat
    10081 catgttcgaa aactacgttg aaggctttga ggccgcctgc gtagtttccc gtaacgctaa
    10141 aaaattccgt cccggtgata cagcaatgca gcgagcaggt gatgttctgt atcgtccgca
    10201 gcattaccac atgaacattg aggaaggcct cgacctcagc agcaaaacgc caacagcact
    10261 ggttcagcgc cttgttcctt ctgtgttcaa ggagccgaaa aacattctgt acactctgga
    10321 tgcgcgtgaa atgcgtgacc cggaacataa aactgaagct ggtcgcgccg caggtatgcg
    10381 ccttgctgca cagattgact ctgacctgat ttccatggtc acgcagcgtg ctactaacgt
    10441 gatcacaatg gctgactcaa ccactggttc acagggccgt gatttgtgga actgtgcggc
    10501 aggtattgat gccaccatga cggcgattgg tgtaccacag ggtatcaacc gccgctcttt
    10561 ctggaacccc ttcaactaca aagaccttgc tggcgagctt ggtcaccgtg cctatgctca
    10621 gggcgcaacc ctgacagcat acgaaaaagc gcagatccct ccggttgcgt ccttcgatag
    10681 ctacaagacc gatatttctg gtcgtgttcc gaagggtaca gcaacttccc tgacgctggc
    10741 ggctgaacct gcgcacaagg ttgaagcgaa agatgccaac gatatgccag tggataaccg
    10801 acaggggact attacggtat ctgcatctgg gttgcaggtt ggcgatgcgt tcaccattgc
    10861 tggcgtgaat tctgtacacc agatcaccaa agacaccacc gggcagccgc aggtattccg
    10921 cgttctggca gtaagcggaa cgacagtaac tatctccccg aaaattctgc cgcctgacaa
    10981 cgcagatgtc gccagccgtc catatgtaaa cgttgatgct aacgcggcaa atggtgcagc
    11041 aattaccatt ctcaacaaga atgccgcacc ggctaacctg ttctgggctg atggttctgt
    11101 tgagctgatg tacggcaaac tggcgttccc gactggtcag ggtccacagg taatgacagc
    11161 aaccaccgag cagggcgcta cgctgatcat gtcttacgcc ttcgaccaca tcaaaggcgt
    11221 aaccactgct cgtttcacca ctctgtacgg ttgctctgta cttgttcctg aatatacggg
    11281 catcgttatt gccgggcagt aattttggtg gggcttcggc cccattttta ttgggagaag
    11341 acaatggcac gaacaatgct ctataagcct ggcaacatga tcacctgtgg tcagtttgct
    11401 gtcgattaca tcattgttga tgacgaagaa gttaaatctc acctgaaaaa aggctgggta
    11461 aaaactcctg aagaaaccgc aacgaagcat aaagtggcta aggcggaaga agatggcgaa
    11521 aacgaagggt gatctcgttc ttaaggcttt acgaaaagcc gggctgtatt ccaatgccac
    11581 gttgacagat gctgaccctc aggcaattga agatgccatt aatgacctcg aagacatgat
    11641 ggcagcatgg caggctaaag gtatcgagct tgggtatcag tttgctgata cagaaaacgg
    11701 catcatgccg ttaccggacg atgattcagg tatccctgca tgggcaaatg atggcgtcgc
    11761 tttgaaactc gctgtgcaag tgtgcatgga taacgtcatt cagccgtcag acgctctcct
    11821 taccgctgct gacagtgcat atcagacaat ctgcatcgct ttaaccaaaa taccaccact
    11881 tgagcggcga aatgacatgc ctcgcggtag tggtaacaaa agcgcgttta cgtggaatcg
    11941 gttttacatc gagaaagatg atccgagtac gtgaggtgaa taaatgccga ttcagcaact
    12001 tccgcttatg aaaggtgtcg gcaaagactt tcgaaacgcc gactatatcg actatctgcc
    12061 agtgaatatg ctggctacac ccaaagaaat cctgaacagc agcggatatc ttcgctcatt
    12121 cccgggcatt gccaaacgtt ctgatgtgaa tggtgtatct cgcggtgtcg agtacaacat
    12181 ggcgcagagt gctgtttatc gcgtgtgtgg tgggaagctc tacaaaggcg aaagcgaagt
    12241 cggtgacgtc gctggaagtg gtcgcgtatc aatggcgcat ggtcggacat ctcaggctgt
    12301 aggcgttaat ggtcaactgg tcgagtatcg ctatgatggc acggttaaaa ccgtctcaaa
    12361 ctggcctaca gacagcggat tcacacagta tgagttaggc tcagtccgcg atattacgcg
    12421 cttgcgtggg cgttatgcgt ggtcaaaaga cggtactgat tcatggttta tcactgacct
    12481 tgaagacgaa tcacatcctg accgttacag cgcacaatat cgtgccgagt ctcagcctga
    12541 cggcatcatc ggcataggaa catggcgaga cttcatcgtc tgctttggtt catcgacgat
    12601 tgaatatttc tccctgactg gggcaaccac cgttggtgcc gctttgtatg tcgcacagcc
    12661 atcgctgatg gtgcagaaag gtattgccgg gacttactgc aaaacaccat tcgctgattc
    12721 ttatgcgttc atcagcaatc cggcaacagg tgcgccgtct gtgtatatca tcggctccgg
    12781 tcaggtatca ccaatcgcca gcgcgagcat tgagaaaatc cttcgttcct acactgctga
    12841 tgaactggct gatggtgtga tggaatcgct gcgatttgat gctcatgagt tgctgattat
    12901 ccatcttccg cgccatgtcc tcgtatacga cgcatcttca agcgccaatg gtccacaatg
    12961 gtgtgtactg aaaacaggcc tgtatgacga tgtgtaccgc gctatcgact tcatttacga
    13021 agacaatcag ataacgtgcg gcgataagct ggaatcggtt accggcaaat tgcagttcga
    13081 tatcagcagc cagtacgaca agcaacagga acacctgctg tttactccgt tgttcaaagc
    13141 ggataacgcc agagttttcg accttgaagt tgaatcgtca actggagttg cgcagtatgc
    13201 tgaccgcctt tttctctctg caaccactga cggcataaat tacgggcgtg agcagatgat
    13261 tgagcagaat gaaccgttcg tttacgacaa acgcgttttg tggaagcgtg tcgggcgaat
    13321 caggaaaaat gtcggcttca aattgcgcgt tatcacgaag tcacctgtca ctctgtctgg
    13381 ctgccagata aggatcgagt aatggcggat tcgaatctca atacacctgt tattgttcag
    13441 gcgacgcggc tcgatacatc aatccttcca cgcaatattt tctcgcagtc atatctgctt
    13501 tacgttattg cacagggggc tgatgttggt aatgtggcga acaaggccaa cgaagcaggg
    13561 cagggtgctt atgatgcaca agtcaggaac gatgagcagg atgtgattct ggtcgatcac
    13621 gaaattcgac tggcatcggc tgaagcgaag atacaggacc acgaaacaag gatcactaac
    13681 gcagaagcgg cgatagtcgg ccttgattct cgattaacga cagcagaaaa cgatattgat
    13741 tatctgacgg atgaagttgt ctccattcaa aacacg
//
LOCUS       DAJTOW010000100        13555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTOW010000100 DAJTOW010000000
VERSION     DAJTOW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.100"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(319..582)
                     /gene="rpsT"
                     /locus_tag="OO244_003152"
     CDS             complement(319..582)
                     /gene="rpsT"
                     /locus_tag="OO244_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5664882.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            685..903
                     /locus_tag="OO244_003153"
     CDS             685..903
                     /locus_tag="OO244_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5664883.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            911..1852
                     /gene="ribF"
                     /locus_tag="OO244_003154"
     CDS             911..1852
                     /gene="ribF"
                     /locus_tag="OO244_003154"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5664884.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1895..4711
                     /gene="ileS"
                     /locus_tag="OO244_003155"
     CDS             1895..4711
                     /gene="ileS"
                     /locus_tag="OO244_003155"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5664885.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4711..5205
                     /gene="lspA"
                     /locus_tag="OO244_003156"
     CDS             4711..5205
                     /gene="lspA"
                     /locus_tag="OO244_003156"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5664886.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5292..5741
                     /gene="fkpB"
                     /locus_tag="OO244_003157"
     CDS             5292..5741
                     /gene="fkpB"
                     /locus_tag="OO244_003157"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5664887.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5743..6693
                     /gene="ispH"
                     /locus_tag="OO244_003158"
                     /gene_synonym="lytB"
     CDS             5743..6693
                     /gene="ispH"
                     /locus_tag="OO244_003158"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5664888.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6759..7673
                     /gene="rihC"
                     /locus_tag="OO244_003159"
     CDS             6759..7673
                     /gene="rihC"
                     /locus_tag="OO244_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5664889.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7840..8661
                     /gene="dapB"
                     /locus_tag="OO244_003160"
     CDS             7840..8661
                     /gene="dapB"
                     /locus_tag="OO244_003160"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5664890.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            9117..10265
                     /gene="carA"
                     /locus_tag="OO244_003161"
     CDS             9117..10265
                     /gene="carA"
                     /locus_tag="OO244_003161"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5664891.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            10283..13504
                     /gene="carB"
                     /locus_tag="OO244_003162"
     CDS             10283..13504
                     /gene="carB"
                     /locus_tag="OO244_003162"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5664892.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGMSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3166 a         3400 c         3803 g         3186 t
ORIGIN      
        1 ggagatattt atgaatggtt aaagtatata ggaatttaat tcaaaaatag agtggtctta
       61 agtttaatac ctcaatgtgt atcacaatat ccatattctt tgtgggggag tctggagatt
      121 gagtagatat tcttgttcag aatgtatcag ccgatggttc tacgattctt aagccacgaa
      181 gagttcagat agtacaacgg catgtctctt ttgactatct ggcaaccggc agtgtgttct
      241 ctcacgcatc acaaaagcag caggcataaa aaaacccgct tgcgcgggct ttttcacaaa
      301 gcttcagcaa attggcgatt aagccagttt gttgatctgt gcagtcaggt tagccttatg
      361 acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca cgatcggttg
      421 catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac
      481 tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac gagccttttc
      541 agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac tcccaaatgt
      601 gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct tttgtcaatg
      661 gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga tggcgcagga
      721 ttctaccagc ttgcggggtg tgaatacagc ttttccgcga taaaaattgc agcaggcggt
      781 cagtttcttc ccgtgatttg ctccatggca atgaaaagcc acttctttct gatttcggta
      841 ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt ttcactgttt
      901 tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc cgcaagaagg
      961 gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca
     1021 gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc tttttgaacc
     1081 tcaaccactg gaactgtttg ctaccgataa agccccggca agactgaccc ggctgcggga
     1141 aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc gtttcgacag
     1201 gcgttttgcg gcgttaaccg cgcaaaattt catcagcgat cttttggtga agcatttgcg
     1261 cgtaaaattt cttgccgtag gtgatgattt ccgctttggc gctggtcgtg aaggcgattt
     1321 cttgttatta cagaaagctg gcatggaata cggcttcgat atcaccagta cgcaaacttt
     1381 ttgcgaaggt ggtgtgcgta tcagcagcac cgccgtgcgt caggcgctgg ctgatgacaa
     1441 tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc gtgtagtcca
     1501 cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc tacgccgtca
     1561 ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg aaaagccgtt
     1621 acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc agcagctgga
     1681 agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag tagtgctgcg
     1741 taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag cgcagattgc
     1801 gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt aagcctgtta
     1861 tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat caaccctgaa
     1921 tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac cgggaatgct
     1981 ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa aaggcaaaaa
     2041 aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata ttggtcactc
     2101 ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg gttatgactc
     2161 gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag tagagcaaga
     2221 atacggtaag ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt gccgcgaata
     2281 cgcggcgacc caggttgacg gtcaacgcaa agactttatc cgtctgggcg tgctgggcga
     2341 ctggtcgcac ccgtatctga ctatggactt caaaactgaa gccaacatca tccgcgcgct
     2401 gggcaaaatc atcggcaatg gtcacctgca caaaggcgcg aagccggtgc actggtgcgt
     2461 agactgccgt tctgcactgg cagaagcgga agttgagtat tacgacaaaa cttctccgtc
     2521 cattgacgtc gctttccagg cggtcgatca ggatgcgctg aaagcgaaat ttggcgtaag
     2581 caacgttaac ggcccaatct cgctggtgat ctggaccact acgccgtgga ctctgcctgc
     2641 gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga tcgacggtca
     2701 ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg gcgtgaccga
     2761 ttacaccatt ctcggcacgg taaaaggtgc agagcttgag ctgctgcgct ttacccatcc
     2821 gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctgg atgccggtac
     2881 cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg gtcagaaata
     2941 cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg gcacttatcc
     3001 gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc tgctgcagga
     3061 aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct gctggcgtca
     3121 caaaacgccg atcatcttcc gcgcgacacc gcagtggttc gtcagcatgg atcagaaagg
     3181 tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggctcccgg actggggcca
     3241 ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc gtcagcgcac
     3301 ctggggcgta ccgatgtcac tgttcgtgca caaagacacg gaagagctgc atccgcgtac
     3361 cctcgaactg atggaagaag tggctaaacg cgttgaagtt gacggcatcc aggcgtggtg
     3421 ggatctcgat gcgaaagaga tcctcggcga cgaagctgac cagtatgtga aagtgccgga
     3481 tactctcgac gtatggtttg actccggatc tacccactct tctgttgttg acgtgcgtcc
     3541 ggaatttgcc ggtcacgcag cggacatgta tctggaaggt tctgaccagc accgtggttg
     3601 gttcatgtct tccctaatga tctccaccgc gatgaaaggc aaagcgccgt atcgtcaggt
     3661 actgacccac ggctttaccg tggatggtca gggtcgcaag atgtctaaat ccatcggcaa
     3721 caccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc gtctgtgggt
     3781 ggcatcaacc gactacaccg gtgaaatggc cgtttctgac gagatcctga aacgtgctgc
     3841 cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc tgaatggttt
     3901 tgatccagca aaagatatgg tgaaaccgga agagatggtg gtactggatc gctgggccgt
     3961 aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat acgatttcca
     4021 cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct tctacctcga
     4081 catcatcaaa gaccgtcagt acaccgccaa agcggatagc gtggcgcgtc gtagctgcca
     4141 gactgcgctg tatcacatcg ctgaagcgct ggtgcgctgg atggcaccaa tcctctcctt
     4201 caccgctgat gaagtgtggg gctatctgcc gggcgaacgt gaaaaatacg tcttcaccgg
     4261 tgagtggtac gaaggcctgt ttggtctggc agacagtgaa gcaatgaacg atgcgttctg
     4321 ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag cgcgtgccga
     4381 caagaaagtg ggtggctcgc tggaagcggc agtaaccttg tatgcagaac cggaactggc
     4441 ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct ccggcgctac
     4501 cgttgcagac tataacgacg cacctgctga tgctcagcag agcgaagtac tcaaagggct
     4561 gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc actacaccca
     4621 ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca gcaacgtcgc
     4681 cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc aacagggcta
     4741 cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa atacctgatc
     4801 ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa tctgcattat
     4861 gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg gcagcgttgg
     4921 ttctttgccg gtattgcgat tggtattagc gtgctcctgg cagtgatgat gtatcgctcg
     4981 aaggccacgc agaagctaaa caatatcgct tacgcgctga ttattggcgg cgcgctgggc
     5041 aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt ctacgtcggc
     5101 gactggcact tcgccacctt caaccttgcc gatactgcca tttgtgtcgg tgcggcactg
     5161 atcgtgctgg aagggttttt accttccaaa gcgaaaaaac aataataaat cctgccggat
     5221 gcggcctaca gattgctacg aaatcgtagg ccgcatccgg caaaaatcct caaaaataag
     5281 agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg cacttcacgc
     5341 taaaactcga cgatggcacc accgccgagt ctactcgcaa caacggtaaa ccggcgctgt
     5401 tccgcctggg cgatgcttct ctttctgaag ggctggagca acacctgctg gggctgaaag
     5461 tgggcgacaa aaccaccttc tcgctggagc cagatgcggc gtttggcgtg ccgtcaccgg
     5521 acctgattca gtacttctcc cgccgtgaat ttatggatgc aggcgagcca gaaattggcg
     5581 caatcatgct ttttaccgca atggatggca gtgagatgcc tggtgtgatc cgcgaaatta
     5641 acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc gttcattttg
     5701 atattgaagt gctggaaatc gatccggcac tggaggcgta acatgcagat cctgttggcc
     5761 aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga aaacgcgctg
     5821 gccatttacg gcgcaccgat atatgtccgt cacgaagtgg tgcataaccg ctacgtggtc
     5881 gatagcttgc gcgagcgtgg ggctatcttt attgagcaga ttagcgaagt gccggacggc
     5941 gcgatcctga ttttctccgc acacggtgtt tctcaggcgg ttcgtaacga agcgaaaagc
     6001 cgcgatttga cggtgtttga tgccacctgt ccgctggtga ccaaagtgca tatggaagtt
     6061 gcccgcgcca gtcgccgtgg cgaagaatct attctcatcg gccacgccgg gcacccggaa
     6121 gtggaaggga cgatggggca gtacagcaac cccgaagggg gaatgtatct ggtcgaatcg
     6181 ccagacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt tatgacccaa
     6241 accacgctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa acgcttcccg
     6301 aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg tcaggaagcg
     6361 gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa aaactcctcc
     6421 aactccaacc gtctggcgga gctggcccag cgtatgggca aacacgcgtt tttgattgac
     6481 gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg cgtgactgcg
     6541 ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg cacgtttgca gcagttgggt
     6601 ggtggtgaag ccattccgct ggaaggccgt gaagaaaata ttgttttcga agtgccgaaa
     6661 gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag ttatgcgaaa
     6721 atgccggtct tgttaccggc attttttatg aagaaaacat gcgtttacct atcttcctcg
     6781 atactgaccc cggcattgac gatgccgtcg ccattgccgc cgcgattttt gcccccgaac
     6841 tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa actacccgca
     6901 atgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccag ggagccgctg
     6961 tgccgctggt acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg ggaatggctg
     7021 gctacgactt tgttgagcac aaccgaaagc cactcgggat accggcgttt ctggcgattc
     7081 gggatgccct gatgcgtgca ccagagcccg ttaccctggt ggccatcggc ccgttaacca
     7141 atattgcgct gttactttta caatgcccgg aatgtaaacc gtatattcgt cgtctggtga
     7201 tcatgggcgg ttctgctggt cgcggcaact gtacaccaaa tgccgagttt aatatcgctg
     7261 ccgatccaga agcggctgcc tgtgtcttcc gtagcggcat cgaaattgtc atgtgcggtc
     7321 tggatgtcac caatcaggca atattaaccc ctgactatct cgctacttta ccggagttga
     7381 atcgcaccgg aaaaatgctc cacgccctgt ttagccacta ccgtagcggc agtatgcaaa
     7441 gcggtctgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg gacttattca
     7501 ccctcaagcc gtgttttgtc gcggtagaaa ctcaaggcga atttacctca ggcacgacgg
     7561 tggttgatat cgacggttgc ctgggcaaac cagccaatgt acaggtggca ttggatctga
     7621 atgtaaaagg cttccaacag tgggtggctg aggtgctggc tctggtgccg taacctgtca
     7681 catgttatcg gcatgcagtc attcatcgac tcatgccttt cactgatatc cctccctgtt
     7741 tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc gctggttact
     7801 ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc aaacatccgc
     7861 gttgccatcg cgggagccgg ggggcgtatg ggccgccagt tgattcaggc ggcactggca
     7921 ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt actgggcagc
     7981 gacgccggtg agctggctgg tgcagggaaa acaggcgtta ccgtgcaaag cagtcttgat
     8041 gcgataaaag atgattttga tgtgtttatc gattttaccc gtccggaagg tacgctgaac
     8101 catctcgctt tttgtcggca gcatggcaaa gggatggtga tcggcactac ggggtttgac
     8161 gaagccggta aacaggcaat tcgtgacgcc gccgccgata ttgcgattgt ctttgccgcc
     8221 aattttagcg ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc caaagtgatg
     8281 ggtgactaca ccgatatcga aattattgaa gcacatcata gacataaagt tgatgcgccg
     8341 tcaggcaccg cactggcaat gggagaggcg atcgcccacg cccttgataa agatctgaaa
     8401 gattgcgcgg tctacagtcg tgaaggccat accggtgaac gtgtgccagg taccattggt
     8461 tttgctaccg tgcgtgcagg tgacatcgtt ggcgaacata ccgcgatgtt tgccgatatt
     8521 ggcgagcgtc tggagatcac ccataaggcg tccagccgca tgacatttgc taacggtgcg
     8581 gtaagatcgg ctttgtggtt gagtggtaag gaaagtggtc tttttgatat gcgagatgtg
     8641 cttgatctca ataatttgta accacaaaat gtttattatg atgcaaaagt aacacattta
     8701 atttattaat tataaagggc tttaattttt agccctttta tttttggtgt tatgctttta
     8761 aattttctat aactgccaaa aattacatgt ttcgtcttct atttttgttg ttttaatgta
     8821 aattttgacc atttggtcca ctttttcctg ctcgttttta tttcatgcaa tcttcttgct
     8881 gcgcaagcgt tttccagaac aggttagatg atgtttttgt cgcttaatgc ctgtaaaaca
     8941 tgcatgagcc acaaaataat ataaaaaatc ctgccattaa gttgactttt agcgcccata
     9001 tctccagaat gccgccgttt gccagaaatt cgtcggtaag cagatttgca ttgatttacg
     9061 tcatcactgt gaattaatat gcaaataaag tgagtgagta ttctctggag ggtgttttga
     9121 ttaagtcagc gctattggtt ctggaagacg gaacccagtt tcacggtcgg gccatagggg
     9181 caacaggttc ggcggttggg gaagtcgttt tcaatacttc aatgaccggt tatcaagaaa
     9241 tcctcactga tccttcctat tctcgtcaaa tcgttactct tacttatccc catattggca
     9301 atgtcggcac taatgacgcc gatgaagaat cttctcaggt acatgcacaa ggtctggtga
     9361 ttcgcgacct gccgctgatt gccagcaact tccgtaatac cgaagacctc tcttcttacc
     9421 tgaagcgcca taacatcgtg gcgattgccg atatcgatac ccgtaagctg acgcgtttac
     9481 tgcgcgagaa aggcgcacag aatggctgca ttatcgcagg cgataacccg gatgcggcgc
     9541 tggcgttaga aaaagcccgc gcgttcccag gtctgaacgg catggatctg gcaaaagaag
     9601 tgaccaccgc agaagcctat agctggacac aagggagctg gacgttgacc ggcggcctgc
     9661 cagaagcgaa aaaagaagac gagctgccgt tccacgtcgt ggcttatgat tttggtgcca
     9721 agcgcaacat cctgcggatg ctggtggata gaggctgtcg cctgaccatc gttccggcgc
     9781 aaacttctgc ggaagatgtg ctgaaaatga atccagacgg catcttcctc tccaacggtc
     9841 ctggcgaccc ggccccgtgc gattacgcca ttaccgccat ccagaagttc ctcgaaaccg
     9901 atattccggt attcggcatc tgtctcggtc atcagctgct ggcgctggcg agcggtgcga
     9961 agactgtcaa aatgaaattt ggtcaccacg gcggcaacca tccggttaaa gatgtggaga
    10021 aaaacgtggt gatgatcacc gcccagaacc acggttttgc ggtggatgaa gcaacattac
    10081 ctgcaaacct gcgtgtcacg cataaatccc tgttcgacgg tacgttacag ggcattcatc
    10141 gcatcgataa gccggcgttc agcttccagg gtcaccctga agccagccct ggtccacacg
    10201 acgccgcgcc gttgttcgac cactttatca agttaattga gcagtaccgt aaaaccgcta
    10261 agtaatcagg agtaaaagag ccatgccaaa acgtacagat ataaaaagta tcctgattct
    10321 gggtgcgggc ccgattgtta tcggtcaggc gtgtgagttt gactactctg gcgcgcaagc
    10381 atgtaaagcc ctgcgcgaag agggttaccg cgtcattctg gtgaactcca acccggcgac
    10441 catcatgacc gacccggaaa tggccgatgc gacctacatc gagccgattc actgggaagt
    10501 ggtacgcaag attattgaaa aagagcgccc ggacgcggtg ctgccaacca tgggcggtca
    10561 gacggcgctg aactgcgcgc tggagctgga acgtcagggc gtgttggaag agttcggtgt
    10621 caccatgatt ggtgccactg ccgatgcgat tgataaagca gaagaccgcc gtcgtttcga
    10681 cgtagcgatg aagaaaattg gtctggaaac cgcgcgttcc ggtatcgcac acacgatgga
    10741 agaagcgctg gctgttgccg ctgacgtggg cttcccgtgc attattcgcc catcctttac
    10801 catgggcggt agcggcggcg gtatcgctta taaccgtgaa gagtttgaag aaatttgcgc
    10861 ccgcggtctg gatctctctc cgaccaaaga gttgctgatt gatgagtcgc tgatcggctg
    10921 gaaagagtac gagatggaag tggtgcgtga taaaaacgac aactgcatca tcgtctgctc
    10981 tatcgaaaac tttgatgcga tgggcatcca caccggtgac tccatcactg tcgcgccagc
    11041 ccaaacgctg accgacaaag aatatcaaat catgcgtaac gcctcgatgg cggtgctgcg
    11101 tgaaatcggc gttgaaaccg gtggttccaa tgtccagttt gcggtgaacc cgaaaaacgg
    11161 tcgcctgatt gttatcgaaa tgaacccacg cgtgtcccgt tcttcggcgc tggcgtcgaa
    11221 agcgaccggt ttcccgattg ctaaagtggc ggcgaaactg gcggtgggtt acaccctaga
    11281 cgaactgatg aacgacatca ctggcggacg tactccggcc tccttcgagc cgtccatcga
    11341 ctatgtggtt actaaaattc ctcgcttcaa cttcgaaaaa ttcgccggtg ctaacgaccg
    11401 tctgaccact cagatgaaat cggttggcga agtgatggcg attggtcgca cgcagcagga
    11461 atccctgcaa aaagcgctgc gcggcctgga agtgggcgca actggcttcg acccgaaagt
    11521 gagcctggat gacccggaag cgttaaccaa aatccgtcgc gaactgaaag acgcaggcgc
    11581 agagcgtatc tggtacatcg ccgatgcttt ccgcgcgggc atgtctgtgg acggcgtctt
    11641 caacctgact aacattgacc gctggttcct ggtacagatt gaagaactgg tgcgtctgga
    11701 agagaaagtg gcggaagtgg gcatcactgg cctgaacgct gaattcctgc gccagctgaa
    11761 acgcaaaggc tttgccgatg cgcgcttggc aaaactggcg ggcgtacgcg aagcggaaat
    11821 ccgtaagctg cgtgaccaat atgacctgca cccggtctac aagcgcgtgg atacctgtgc
    11881 ggcagagttt gccaccgaca ccgcttacat gtactccact tatgaagaag agtgcgaagc
    11941 gaatccgtct accgaccgtg aaaaaatcat ggtgcttggc ggcggtccaa accgtatcgg
    12001 tcagggtatc gaattcgact actgctgcgt acacgcctcg ctggcgctgc gtgaagacgg
    12061 ttacgaaacc attatggtta actgtaaccc ggaaaccgtc tctaccgact acgacacttc
    12121 cgatcgcctc tacttcgagc cggtaactct ggaagatgtg ctggaaatcg tgcgtattga
    12181 gaagccgaaa ggcgttatcg ttcagtacgg cggtcagacc ccgctgaaac tggcgcgtgc
    12241 actggaagcc gctggcgtac cggttatcgg caccagcccg gatgctatcg accgtgcgga
    12301 agaccgtgaa cgcttccagc atgcggttga ccgtctgaaa ctgaaacaac cggcgaacgc
    12361 caccgttact gctattgaaa tggcagttga gaaggcaaaa gagattggct acccgctggt
    12421 ggtgcgtccg tcttacgttc tcggcggtcg ggcgatggaa atcgtctatg acgaagctga
    12481 cctgcgtcgc tacttccaga cggcggttag cgtgtctaac gatgcgccag tgttgctgga
    12541 tcatttcctt gatgacgcag tagaagttga cgtggatgcc atctgcgacg gcgaaatggt
    12601 gctgattggc ggcatcatgg agcacatcga gcaggcgggt gtgcactccg gtgactccgc
    12661 atgttcgctg ccagcgtaca ccttaagtca ggaaattcag gatgtgatgc gccagcaggt
    12721 gcagaaactg gccttcgaat tgcaggtgcg cggtctgatg aacgtgcagt ttgcggtgaa
    12781 aaacaacgaa gtttacctga ttgaagtaaa cccgcgtgcg gcgcgtactg ttccgttcgt
    12841 ctccaaagcc accggcgtac cgctggcaaa agtggcggcg cgtgtgatgg ctggcaaatc
    12901 gctggctgag cagggcgtaa ccaaagaagt tattccgccg tactactcgg tgaaagaagt
    12961 ggtgctgccg ttcaataaat tcccaggcgt tgacccgctg ttagggccag aaatgcgctc
    13021 taccggggaa gtcatgggcg tgggccgcac cttcgctgaa gcgttcgcca aagcgcagct
    13081 gggcagcaac tccaccatga agaaacacgg tcgtgcgctg ctttccgtgc gcgaaggcga
    13141 taaagaacgc gtggtggacc tggcggcaaa actgctgaaa cagggcttcg agctggatgc
    13201 gacccacggc acggcgattg tgctgggcga agcgggtatc aatccgcgtc tggtaaacaa
    13261 ggtgcatgaa ggccgtccgc acattcagga ccgtatcaag aatggcgaat atacctacat
    13321 catcaacacc acctcaggcc gtcgtgcgat tgaagactcc cgcgtgatcc gtcgcagtgc
    13381 gctgcaatat aaagtgcatt atgacaccac cctgaacggt ggtttcgcta ccgcgatggc
    13441 gctgaatgcc gatgcgactg aaaaagtaat ttcggtgcag gaaatgcacg cacagatcaa
    13501 ataatagcgt gtcgtggcgg atatttttca tccgctaatt tgatcgaata actaa
//
LOCUS       DAJTOW010000101        12908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTOW010000101 DAJTOW010000000
VERSION     DAJTOW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.101"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO244_003163"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO244_003163"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5664893.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO244_003164"
                     /pseudo
     CDS             complement(1806..3224)
                     /locus_tag="OO244_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3378..3722)
                     /locus_tag="OO244_003165"
     CDS             complement(3378..3722)
                     /locus_tag="OO244_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5664894.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO244_003166"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO244_003166"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5664895.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO244_003167"
     CDS             complement(4712..5647)
                     /locus_tag="OO244_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5664896.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO244_003168"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO244_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5664897.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO244_003169"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO244_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5664898.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO244_003170"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO244_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5664899.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO244_003171"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO244_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5664900.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO244_003172"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO244_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5664901.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO244_003173"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO244_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5664902.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO244_003174"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO244_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5664903.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO244_003175"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO244_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5664904.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO244_003176"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO244_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5664905.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3299 c         2951 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 tcaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaact agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAJTOW010000102        12888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTOW010000102 DAJTOW010000000
VERSION     DAJTOW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.102"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..893)
                     /locus_tag="OO244_003177"
     CDS             complement(<1..893)
                     /locus_tag="OO244_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5664906.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKADVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="OO244_003178"
     CDS             1134..1934
                     /locus_tag="OO244_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5664907.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO244_003179"
     CDS             2012..2782
                     /locus_tag="OO244_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5664908.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO244_003180"
     CDS             complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO244_003180"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5664909.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO244_003181"
     CDS             complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO244_003181"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5664910.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5037..5759
                     /locus_tag="OO244_003182"
     CDS             5037..5759
                     /locus_tag="OO244_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5664911.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO244_003183"
     CDS             complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO244_003183"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5664912.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO244_003184"
     CDS             6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO244_003184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5664913.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7152..7228
                     /locus_tag="OO244_003185"
     tRNA            7152..7228
                     /locus_tag="OO244_003185"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7186..7188,aa:Asp,seq:gtc)
     gene            complement(7359..8405)
                     /locus_tag="OO244_003186"
     CDS             complement(7359..8405)
                     /locus_tag="OO244_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5664914.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO244_003187"
                     /pseudo
     CDS             complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO244_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO244_003188"
     CDS             complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO244_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5664915.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO244_003189"
     CDS             complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO244_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5664916.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11365..12195)
                     /locus_tag="OO244_003190"
                     /pseudo
     CDS             complement(11365..12195)
                     /locus_tag="OO244_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12182..12888)
                     /gene="tagK"
                     /locus_tag="OO244_003191"
                     /pseudo
     CDS             complement(12182..12888)
                     /gene="tagK"
                     /locus_tag="OO244_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         2955 a         3354 c         3435 g         3144 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccgctttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc
     3061 gcgcagtttg ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct
     3121 tgaaagcgtg tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc
     3181 aaccagcgtc gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc
     3241 tgcatgcttc tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt
     3301 cacgccagcg ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt
     3361 cgccatttca accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt
     3421 tggcagacgt acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt
     3481 aggcacgtac tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc
     3541 acgcgctttg ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc
     3601 tacggtcaga agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc
     3661 ggcagttgtt gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc
     3721 ataattgcgc cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt
     3781 tgcgtgagga tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt
     3841 acgtttttta acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc
     3901 gtggagatag ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc
     3961 cggaattccc atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat
     4021 agacgtccca tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc
     4081 agctgcagaa aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag
     4141 caggacagag gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa
     4201 gacgaccgat cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct
     4261 ttctttgacc ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta
     4321 attccattaa ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt
     4381 ttcagaatta cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa
     4441 taatcattta tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac
     4501 gtatattcat gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt
     4561 gattgataca tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc
     4621 gtgtcgccgc aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc
     4681 ggtgtggtaa ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact
     4741 acctgtgttg ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac
     4801 ttttccacca gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg
     4861 gcctccggtt gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga
     4921 tcgacaatca ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag
     4981 gcgggaatac tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga
     5041 aaccggcaag agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg
     5101 gagagcttta tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg
     5161 gttttcatct gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg
     5221 tttctcatca agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac
     5281 ttcatcttcc ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt
     5341 ggtgcaccga tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct
     5401 ggctggtgat tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg
     5461 tattgcgcaa aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact
     5521 ggctctcttt gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga
     5581 acaaacacgg aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta
     5641 ttgttgcccg gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca
     5701 agccacgaat tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa
     5761 cttcgacttg gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt
     5821 catcacagcg ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt
     5881 gatgctgccc caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt
     5941 tgtctgctgt tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc
     6001 ggacatactg gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg
     6061 cgacaatagc ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa
     6121 aggttttctc gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac
     6181 ccagacacga accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg
     6241 taattgaatc gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa
     6301 ttacacgcca gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact
     6361 atgaaggcca caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg
     6421 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
     6481 tacatggtat tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
     6541 agtttatgga ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag
     6601 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct
     6661 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc
     6721 tcgatgcgct atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
     6781 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
     6841 tggcttttgc gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca
     6901 ttgtacgtca ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac
     6961 atgaagcccg tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa
     7021 tacatgtaaa aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa
     7081 gatgagaaaa atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg
     7141 gaacacaacg cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc
     7201 gcgggttcga gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg
     7261 ggctttcgtc gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt
     7321 aaaaaatggc cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc
     7381 tccagcagtt ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt
     7441 tctgcctggc gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga
     7501 cggatgacac gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg
     7561 ccggtcatcg gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc
     7621 atctgttcag tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg
     7681 atctccggcg tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg
     7741 gagaagtccg gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg
     7801 gtctctgctg cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc
     7861 agttgctggc gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa
     7921 cgctccacat cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga
     7981 atatccgcca ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc
     8041 gcagcatctt cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg
     8101 tacgtggagc acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc
     8161 tgctggatac ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc
     8221 agacgatggg cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa
     8281 tcgccatatt gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga
     8341 tcgtactcca gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt
     8401 tccatttaat tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgcgggactc
     8461 acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct
     8521 ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg
     8581 tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag
     8641 ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc
     8701 gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc cccacagccc gaggctgtac
     8761 agactgatgt gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag
     8821 gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc
     8881 gccggaacgg tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc
     8941 cagggggggg cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc
     9001 tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg
     9061 tattaatgga aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac
     9121 tgatgccgga aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga
     9181 ccagtagtcc ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc
     9241 tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc
     9301 gcaacgcctg gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata
     9361 actggcggat caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg
     9421 gcgggcggat aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc
     9481 ggccattgcg ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga
     9541 tgtggcgcag attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt
     9601 ccgagcccgt atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg
     9661 gctcccggcg cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt
     9721 agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga
     9781 ccgagaacac ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg
     9841 tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac
     9901 agaacaggct gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt
     9961 tcagcaggat aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc
    10021 ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat
    10081 gaccgtgaaa cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt
    10141 acataacggg ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag
    10201 ccaggggcgc gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct
    10261 cgccgcagag ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac
    10321 caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc
    10381 ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg
    10441 gccacagcgt tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa
    10501 tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt
    10561 ccgtatcggg atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc
    10621 tgaccacttc cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg
    10681 tgcgggcggt caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat
    10741 ccgttccctg catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc
    10801 ccgtttcccg cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg
    10861 gtctctctct gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta
    10921 cccggaatgc agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg
    10981 tgttatggcc actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt
    11041 tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact
    11101 tgtgctcata cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg
    11161 ccgcgtacgg ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa
    11221 atgacaaatc ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt
    11281 cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat
    11341 tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca
    11401 ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag
    11461 cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag
    11521 ggagtcggca gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc
    11581 gccattgttc agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg
    11641 cggcgatttc agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat
    11701 cagttcgagt accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc
    11761 attccactgc ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc
    11821 ttcctgcgct tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc
    11881 gggcggcggg agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat
    11941 ctcacagcgg atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg
    12001 ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg
    12061 aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat
    12121 ctgaatgccc tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc
    12181 gttattgttt gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt
    12241 caatgccaat gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg
    12301 tttccggggc gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag
    12361 gccagatagt ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta
    12421 acccaccagt gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat
    12481 ccagatcctg cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat
    12541 cttgttcggg agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc
    12601 ctttttccgc accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat
    12661 tttgtgatgt cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt
    12721 ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct caggttcgga gatgatggat
    12781 tgccatgtac aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac
    12841 gcatacggtg atgaggttcc acaatctggt cgtttaccgt acagcaat
//
LOCUS       DAJTOW010000103        12674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTOW010000103 DAJTOW010000000
VERSION     DAJTOW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.103"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..446)
                     /locus_tag="OO244_003192"
     CDS             complement(<1..446)
                     /locus_tag="OO244_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR5664917.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLES"
     gene            complement(706..1326)
                     /gene="sodA"
                     /locus_tag="OO244_003193"
     CDS             complement(706..1326)
                     /gene="sodA"
                     /locus_tag="OO244_003193"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5664918.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1611..2645
                     /gene="rhaT"
                     /locus_tag="OO244_003194"
                     /pseudo
     CDS             1611..2645
                     /gene="rhaT"
                     /locus_tag="OO244_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2642..3508)
                     /gene="rhaR"
                     /locus_tag="OO244_003195"
     CDS             complement(2642..3508)
                     /gene="rhaR"
                     /locus_tag="OO244_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5664919.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3564..4400)
                     /gene="rhaS"
                     /locus_tag="OO244_003196"
     CDS             complement(3564..4400)
                     /gene="rhaS"
                     /locus_tag="OO244_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5664920.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4688..6157
                     /gene="rhaB"
                     /locus_tag="OO244_003197"
     CDS             4688..6157
                     /gene="rhaB"
                     /locus_tag="OO244_003197"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5664921.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6154..7413
                     /gene="rhaA"
                     /locus_tag="OO244_003198"
     CDS             6154..7413
                     /gene="rhaA"
                     /locus_tag="OO244_003198"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5664922.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            7655..8479
                     /gene="rhaD"
                     /locus_tag="OO244_003199"
                     /pseudo
     CDS             7655..8479
                     /gene="rhaD"
                     /locus_tag="OO244_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8489..8803
                     /gene="rhaM"
                     /locus_tag="OO244_003200"
     CDS             8489..8803
                     /gene="rhaM"
                     /locus_tag="OO244_003200"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5664923.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9104..9529
                     /locus_tag="OO244_003201"
     CDS             9104..9529
                     /locus_tag="OO244_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5664924.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSGEED
                     QVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9540..10991
                     /locus_tag="OO244_003202"
     CDS             9540..10991
                     /locus_tag="OO244_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5664925.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            10981..12051
                     /locus_tag="OO244_003203"
     CDS             10981..12051
                     /locus_tag="OO244_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5664926.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALREKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12051..>12674
                     /locus_tag="OO244_003204"
     CDS             12051..>12674
                     /locus_tag="OO244_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5664927.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3002 a         3298 c         3350 g         3024 t
ORIGIN      
        1 gactccaacg acatcaacac aaatgcccca gcttcttctt tcgtaccgta ctgctgcatg
       61 atggaagcgt aaagtccgcc gttggtcata tccatcgccg ccaccagcgc cagcgttgaa
      121 agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc
      181 gcaaccaccc acgcgacggc aattttagtt accaccagcg taccggattt acgcagtacc
      241 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
      301 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
      361 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
      421 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
      481 tgcgtaattc ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat
      541 caataaaagt gatcaaaatc acatcaattg aaacgctgtt ttatttttat aggaccattg
      601 tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
      661 gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt ttttcgccgc
      721 aaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttga tgtagtccgg
      781 acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccagaatcgg
      841 gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt tagcagtaga
      901 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
      961 agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat
     1021 agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc agaacaggct
     1081 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
     1141 gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca ggctttccag
     1201 cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
     1261 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
     1321 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg
     1381 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg
     1441 gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta atgccgtgat
     1501 tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca cgatttttac
     1561 actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag atgagtaacg
     1621 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
     1681 ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc ggtgggattg
     1741 tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat ttctgggcgt
     1801 attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
     1861 ggatcggtaa tatcaactat ggcctgacca tgcgttatct cggcatgtcg atgggaattg
     1921 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
     1981 atttcgatgt gctgattaac accgaaggcg gacgcatgac gttactcggc gttctggtgg
     2041 cgctgattgg cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc aagatgggca
     2101 ttaaagccga agagttcaat ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt
     2161 tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg
     2221 cacttggcgt cgatccactg tatgttgctc tgccaagcta tgttgtcatc atgggcggcg
     2281 gcgcgatcat taacctcggt ttctgtttta ttcgtctggc aaaagtgaag gatttgtcgc
     2341 taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta ctctcggcac
     2401 tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctagggccac gcccgcattc
     2461 cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta ttgtgcggcg
     2521 gtatcgtcgg gctggtgctg aaagagtgga acaatacagg acgccgtccg gtaacggtgt
     2581 tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc ggcatggcga
     2641 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
     2701 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
     2761 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgccgcagat
     2821 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
     2881 acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc
     2941 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt
     3001 aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc atttcgttag
     3061 caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg
     3121 ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa
     3181 tcgctccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca
     3241 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaata taaaagagat
     3301 cgccacgggt aatgcgataa ggacgatcgt tgagtacatg cagaccatta ccgcgccaga
     3361 caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa gacatcttgc ggataacggt
     3421 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
     3481 gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta
     3541 caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg gcgaatatca
     3601 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
     3661 ccacagcgat aggcgatgtc agtgacgctg gcctcgctgt ggcgtagtag atgtcgggct
     3721 ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca gtcccgtttg ctgcttaagc
     3781 tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc ccaattcacc
     3841 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
     3901 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaagatc
     3961 tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat ctgtgcaacc
     4021 agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg atactgccca
     4081 tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
     4141 cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg ccgatcatga
     4201 tcgcgtacga aacagaccgt gccaccggtg atggtatagg gctgcccatt aaacacatga
     4261 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
     4321 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt tacaagacgg aaaaaaatcc
     4381 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
     4441 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
     4501 tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca
     4561 aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
     4621 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
     4681 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
     4741 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt
     4801 taacaatggg ctgcatagcc aaaacggtta tgtcacctgg gatgtggata gcctggaaag
     4861 tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg atagcattgg
     4921 gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc gtgtgggcct
     4981 gcccgttgct tatcgcgata gccgcaccaa tggcctgatg ccgcaggcac aacagcaact
     5041 cggcaaacgc gatatttatc agcgtagcgg catccagttt ctacccttca atacgcttta
     5101 tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt ccacacattg ctcacgctct
     5161 gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg aatataccaa
     5221 tgccaccacc acacaactgg tcaatatcaa tagcgacgac tgggacgagt cgctactggc
     5281 gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcattgg
     5341 gcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca gccatgatac
     5401 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc tctcttctgg
     5461 cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca cggcgctggc
     5521 agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
     5581 gggcttatgg ctgcttcagc gagtgcttca ggaacggcaa atcaacgatc tcccggcgct
     5641 tatcgccgcg acacaggcac ttccggcctg ccgcttcatc atcaatccca atgacgatcg
     5701 ctttattaat cctgacgaga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
     5761 accgatcccg ggaagtgatg ctgaactggc gcgctgtatt ttcgacagtc tggcgctgct
     5821 gtatgccgat gtgttgcatg agctggcgca gctgcgcggt gaagatttct cgcaactgca
     5881 tattgtcggc ggcggctgcc agaacacgct gctcaaccag ctatgcgccg atgcctgcgg
     5941 tattcgggtg atcgccgggc ctgttgaagc ctcaacgctc ggcaatatcg gcatcgagtt
     6001 aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca gcaccaccgc
     6061 gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcagat
     6121 tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg
     6181 gagctggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa
     6241 cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa
     6301 aacccggaag gttcgctgac cggggggatt caggccacag gcaattatcc gggcaaagcg
     6361 cgtaatgcca gtgagctacg tgccgatctg gaacaggcta tgcggctgat tccggggccg
     6421 aaacggctta atttacatgc catctatctg gaatcagata cgccagtctc gcgcgaccag
     6481 atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
     6541 gattttaacc cctcctgctt ttcgcatccg ctaagcgccg atggctttac gctctcgcat
     6601 cccgacgaca gcattcgcca gttctggatt gatcactgca aagccagccg tcgcgtttcg
     6661 gcctattttg gcgagcaact cggcacacca tcggtgatga acatctggat cccggatggt
     6721 atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc agcactggat
     6781 gaggtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga gagcaaattg
     6841 tttggcattg gcgcagagag ctacacggtt ggctccaatg agttttacat ggggtatgcc
     6901 accagccgcc agactgcgct gtgcctggac gccgggcact tccacccgac tgaagtgatt
     6961 tccgacaaga tttccgccgc catgctgtat gtaccgcagt tgctgctgca cgtcagccgt
     7021 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcaattgcc
     7081 agtgagattg tgcgtcacga tctgtttgac cgggtgcata tcggccttga cttcttcgat
     7141 gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa aaaagccctg
     7201 ctgcgtgcgt tgctggaacc taccgctgag ctgcgcaagc tggaagcggc gggcgattac
     7261 actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgcggcaggc ggtctgggaa
     7321 atgtattgcc aacatcacga tacgccagca ggtagcgaat ggctggagag cgtgcgggct
     7381 tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc acgagcgcct
     7441 tatccggcct acgggtcgaa tgtccatgcg ggctgtcacc ctcaccccgg ccctctccct
     7501 gaaagggcga gggagaaaaa catagcctcg tgcggcaacg gagctgaaca ccgctccgga
     7561 cagtcccctc gcccctacgg ggagagggtt agggtgaggg gaacaagccg caccatgtct
     7621 aaacaaggca acctaattta caggaacaca gaacatgcaa aacattaccc agtcctggtt
     7681 tgtccaggga atgatcaaag ccaccaccga tgcctagctg aaaggctggg acgagcgcaa
     7741 cggcggcaac ctgacgctgc gcctggatga cgccgatatc gcgctttatc acgacaactt
     7801 ccacccacaa ccgcgctata ttccgctcag ccaacccatg cctttactgg caaatacgcc
     7861 gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagctcgatc ctgcagctaa
     7921 cttaggcgtc gtaaaagtcg acagcgatgg cgcgggctac catattctct gggggttaac
     7981 caacgaagcc gttccaactt ctgaacttcc ggctcacttt ctctcccact gcgagcgcat
     8041 taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca acctgatcgc
     8101 cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt gggaaggcag
     8161 caccgaatgt ctggtagtat tcccggatgg cgttggcatt ctgccgtgga tggtgcccgg
     8221 cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc tggtgttgtg
     8281 gcccttccac ggtgtgttcg gttccggacc gacgctggat gaagcctttg gtttaatcga
     8341 caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg gcatgaaaca
     8401 gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta caccactagc
     8461 cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca tgcaggtaaa
     8521 ccccgacgcc cacgaagagt atcagcgtcg gcataatccc atctggccgg aactggaagc
     8581 agtgttgaaa tctcacggtg tgcataacta cgccatttac ctcgacaaag cgcgtaatct
     8641 gctgtttgcc atggtagaga ttgaatctga agaacggtgg aatgcggtag ccagtactga
     8701 tgtttgccaa cgttggtgga aatatatgac cgatgttatg cccgctaacc cagataacag
     8761 cccggtgagt agcgagctgc aagaagtgtt ttacctgccg taattgccca atgtgccgga
     8821 gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa ttaaacatat tacatctccc
     8881 ggtaagttat atttccctga tacattgtga gtaaatcaca aaaataatga ataacccatt
     8941 aatgattcat gtggtttatt taaataacca ttatgtgcat tactccgcaa atctgacctt
     9001 tcactctgtc tcaaattgtc attgtttacc gacatatcgc ctctcatcac ttaatcgctc
     9061 caccattaca attgattaaa atatatttta tggagtccga ttaatggcag ctcttactgc
     9121 aagctgtatt gacctgaata ttcagggcaa tggcgcttat tccgttctga agcagttggc
     9181 gacaatagcg ttacaaaacg gttttatcac cgactcacac cagttcctgc aaaccctgct
     9241 gctgcgcgaa aaaatgcact ctactggatt tcacggtaaa agcgcctgcg ttaaacaacc
     9301 gttcgtatta ttcgcccgca aagcgcaggc tattgactgg aaagccagcg atggcgaaga
     9361 cgtcaattgc tggatctgcc tcggcgtgcc gcaaagcggc gaagaggatc aggtcaaaat
     9421 catcggcaca ctgtgtcgca aaattattca ccaggaattt attcatcaac tgcaacaggg
     9481 cgataccgac caggtgctgg ccttgttaaa tcaaaccctc agctcataag gaagtggcga
     9541 tggagtcatc cttacgtatt gtcgcgatca ccaactgccc cgccgggatc gctcacacct
     9601 acatggtggc ggaagccctg gaacagaaag cccgttctct cggccatacc ataaaagtgg
     9661 aaactcaagg gtccagtggc gttgaaaacc gcttatccag cgaagagatt gccgctgccg
     9721 attacgtcat tcttgctacc gggcgtggcc tgagcggtga tgatcgcgcg cggtttgccg
     9781 ggaagaaagt ttatgagatt gccatctccc tggcgttgaa aaatatcgac cagattttca
     9841 gcgaattacc gacaaactcg cagctttttg ccgcagatag cggcgtgaag cttggcaaac
     9901 aggaagtgca aagcggcagc gtgatgagcc acctgatggc gggcgtctct gcggcgctgc
     9961 cgtttgtcat cggcggtggt atcctggtgg cgcttgccaa catgctggtg cagttcggtt
    10021 tgccgtatac cgatatgtcg aaaggcgcgc cgtcgtttac ctgggtagtt gaatccatcg
    10081 gttaccttgg cttcaccttt atgatcccca tcatgggcgc ttatattgcc tcgtcgattg
    10141 ccgataaacc ggctttcgct ccggcgtttc tggtttgcta tctggcgaac gacaaagcgc
    10201 tgctcggcac ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg ctggcgattg
    10261 gctatttcgt cttctggttc cgcaaagtgc gccttggcaa agcattacaa ccgctgctcg
    10321 gttcaatgct gatcccgttc gttactctgc tggttttcgg cgtgctcact tactacgtta
    10381 tcgggccggt gatgtccgac ctcatgggcg ggctgctcca cttcctgaat accattcctc
    10441 catcaatgaa gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg
    10501 gccccatcaa taaaaccgcc tggttcttct gcttctcact gctggaaaaa cacatttatg
    10561 actggtacgc catcgtcggc gttgtcgcgt tgatgccgcc cgttgcggcg ggtcttgcga
    10621 cgttcattgc gcccaaactg tttacccgac aggaaaaaga ggctgccagt agtgccattg
    10681 tggtcggtgc cacagtcgca accgaaccgg ctatacctta cgctctggca gctccgttgc
    10741 cgatgattac cgccaatacg ctggcgggcg gcattaccgg cgtgctggtg atcgctttcg
    10801 gaatcaaacg tctggcacct ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc
    10861 cggtaggttc gttttatctg gtgctggcga ttggcctcgc gctgaacatt tcattcatta
    10921 tcgtcctgaa aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc
    10981 atgaacattg agttactgca acagttgtgc gaagccagcg ccgttagcgg cgatgaacag
    11041 gaagttcgcg acatcctgat aaacacgctg gaaccttgcg ttaatgaaat cacctttgat
    11101 ggtctgggca gctttgttgc ccgtaaggga aataaaggtc caaaagttgc cgttgtcggg
    11161 catatggatg aagtcggctt tatggtcacc cacatcgacg agagcggttt tctgcgtttc
    11221 accaccattg gtggctggtg gaatcagtcg atgctcaacc atcgggtaac catacgcaca
    11281 cacaagggag tgaaaatccc tggtgtgatt ggtttcgtcg cgcctcatgc gttaagggaa
    11341 aagcaaaggc aacagccgct gtcatttgat gagatgttca ttgatattgg cgcaaacagt
    11401 cgcgatgagg tggaaaaacg cggtgtggcg atgggcgatt ttattagccc ggaagccaat
    11461 tttgcctgct ggggcgaaga taaagtggtc ggcaaggcgc tggataaccg catcggctgc
    11521 gcaatgatgg ctgaactatt gcagacggtg aataatcccg aaattacgct gtatggcgtc
    11581 ggcagcgtgg aagaagaagt tgggctacgc ggggcacaaa cctcggcgga acacattaaa
    11641 ccggatgtag tgatcgtgct ggataccgct gtcgcaggcg atgttccggg cattgataac
    11701 attaaatacc cgctgaaact gggccagggg ccggggctga tgctgtttga caagcgctac
    11761 ttccccaacc agaaactggt agcagcgtta aaaaactgtg ccgcacataa cgatttaccg
    11821 ctgcaatttt ccaccatgaa aaccggagcg acggatggcg ggcgctacaa cgtgatgggc
    11881 ggcgggcgtc cggttgttgc gctgtgcctg ccaacccgct atctgcacgc taacagcggc
    11941 atgatttcaa aagccgatta cgatgctctg ctcacgttga tacggggttt tctgacgacc
    12001 ttaaccgcgg agaaagtcaa cgcgtttagc cagttccgtc aggtggatta atgctaaacg
    12061 aacgccagtt aaagattgtc gatctgctgg agcaacagcc gcgcacgcct ggtgagctgg
    12121 cgcaacagac tggcgtttca ggcaggacca tcctgcgtga tattgactat ctcaacttta
    12181 cccttaacgg caaagcccgc attttcgcca gtggcagtgc gggctatcag ctggaaatct
    12241 tcgagcgccg cagctttttt cagttgctgc aaaagcacga taacgacgat cggctgctgg
    12301 cgctgttatt actgaatact ttcactcccc gtgcgcaact cgcctcggcg cttaatttgc
    12361 cagaaacgtg ggtagcagag cgtctgcccc agttaaaaca gcgttatgaa cgcacttgtt
    12421 gcctggccag ccgccctggt ttgggccatt tcattgatga gacagaagag aaactcgtta
    12481 tcttgctggc gaacttgctg cgcaaagatc cgtttttaat tccgctggcg ggcataacac
    12541 gagacaacct tcagcattta tccacggcct gcgacaacca acaccgctgg ccgctcatac
    12601 agggtgatta tctctccagc ctgattctgg cgatttacgc cctgcgtaat caactgaccg
    12661 atgagtggcc gcaa
//
LOCUS       DAJTOW010000104        12652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTOW010000104 DAJTOW010000000
VERSION     DAJTOW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.104"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            118..1815
                     /gene="maeA"
                     /locus_tag="OO244_003205"
     CDS             118..1815
                     /gene="maeA"
                     /locus_tag="OO244_003205"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5664928.1"
                     /translation="MEPKTKKQRSLYISYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIQVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1949..2959
                     /gene="adhP"
                     /locus_tag="OO244_003206"
     CDS             1949..2959
                     /gene="adhP"
                     /locus_tag="OO244_003206"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5664929.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3105..3389
                     /locus_tag="OO244_003207"
     CDS             3105..3389
                     /locus_tag="OO244_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5664930.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO244_003208"
     CDS             complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO244_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5664931.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO244_003209"
     CDS             complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO244_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5664932.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO244_003210"
     CDS             complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO244_003210"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7620..7622),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5664933.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8439..9320
                     /gene="yddG"
                     /locus_tag="OO244_003211"
     CDS             8439..9320
                     /gene="yddG"
                     /locus_tag="OO244_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5664934.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9575..10675
                     /gene="ompC"
                     /locus_tag="OO244_003212"
     CDS             9575..10675
                     /gene="ompC"
                     /locus_tag="OO244_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5664935.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10839..11324
                     /locus_tag="OO244_003213"
     CDS             10839..11324
                     /locus_tag="OO244_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5664936.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERTIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11434..12423
                     /locus_tag="OO244_003214"
     CDS             11434..12423
                     /locus_tag="OO244_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5664937.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3336 a         3126 c         2938 g         3252 t
ORIGIN      
        1 accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac gccctggcgg
       61 taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg
      121 gaaccaaaaa caaaaaaaca gcgttcgctt tatatctctt acgctggccc tgtactgctg
      181 gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac
      241 ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg
      301 atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag
      361 gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct
      421 gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt
      481 tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac
      541 gtgccgaacc ataatattca agtgattgtg gtgactgacg gtgaacgtat tctggggctt
      601 ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc
      661 tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac
      721 caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgat
      781 gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg
      841 ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc
      901 aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca
      961 ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt
     1021 ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa
     1081 ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg
     1141 acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac
     1201 ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt gcgcaatgta
     1261 aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc
     1321 cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtct
     1381 cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc
     1441 actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat cgcccagtgt
     1501 aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg cgcgtcacgt
     1561 atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc
     1621 ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca
     1681 attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa aacgtctgct
     1741 gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc
     1801 cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt ccctctccct
     1861 ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat
     1921 gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga aggatcatca
     1981 tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat
     2041 ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa
     2101 aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt
     2161 cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag gatgcggtca
     2221 ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata
     2281 cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg cggtaaaagt
     2341 gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg tcaccaccta
     2401 caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg
     2461 cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc
     2521 cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag atttagcgat
     2581 taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc
     2641 tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc
     2701 aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg
     2761 tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac
     2821 cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt
     2881 agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt
     2941 gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt cgcagcatcg
     3001 cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg aacaataatg
     3061 atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa atggccaatc
     3121 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     3181 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     3241 cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa
     3301 tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggttgtgt
     3361 cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg ccatttgaaa
     3421 ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcacttc
     3481 aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc atccgggatg
     3541 aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct
     3601 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
     3661 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
     3721 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
     3781 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
     3841 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
     3901 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg
     3961 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
     4021 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
     4081 tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
     4141 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
     4201 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
     4261 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
     4321 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
     4381 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
     4441 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
     4501 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc
     4561 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
     4621 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
     4681 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
     4741 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
     4801 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
     4861 cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat caccgtccag
     4921 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
     4981 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
     5041 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
     5101 gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg ttcgccccct
     5161 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
     5221 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
     5281 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
     5341 cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac agtgacacga
     5401 tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac aaactgttcc
     5461 ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
     5521 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt
     5581 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
     5641 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
     5701 aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt acccggtgcg
     5761 gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
     5821 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
     5881 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
     5941 gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt gtagatccag
     6001 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
     6061 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata gccgttgttc
     6121 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
     6181 atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag
     6241 atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag
     6301 tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc
     6361 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
     6421 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
     6481 agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac cgggttaaag
     6541 ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata cttgatgacg
     6601 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
     6661 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
     6721 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
     6781 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
     6841 tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
     6901 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
     6961 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
     7021 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca
     7081 acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga
     7141 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
     7201 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
     7261 aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
     7321 ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc cgtaccggaa
     7381 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
     7441 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
     7501 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
     7561 tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     7621 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgggtc
     7681 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
     7741 tttaccgtta cgccctgctc gttcttttca ataaagttag catcacggtc agctttcatc
     7801 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
     7861 ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc cagcaaaccg
     7921 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
     7981 taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa tagcccgcaa
     8041 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
     8101 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
     8161 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
     8221 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
     8281 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
     8341 tagcggtaga ataacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
     8401 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
     8461 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
     8521 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
     8581 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
     8641 ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg tatgcggcga
     8701 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
     8761 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
     8821 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
     8881 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
     8941 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
     9001 tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag
     9061 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
     9121 ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag
     9181 gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
     9241 tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc
     9301 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
     9361 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
     9421 gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc
     9481 attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
     9541 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
     9601 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
     9661 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
     9721 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
     9781 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
     9841 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
     9901 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
     9961 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
    10021 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
    10081 tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt atggtgctga
    10141 cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
    10201 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc
    10261 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
    10321 tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg
    10381 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
    10441 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
    10501 cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
    10561 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
    10621 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
    10681 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
    10741 aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat
    10801 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
    10861 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
    10921 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
    10981 gtgaagaaag aacaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
    11041 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
    11101 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
    11161 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac
    11221 tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa aatcagcatt
    11281 cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat
    11341 aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa
    11401 gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat
    11461 catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa
    11521 aacactacaa aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac
    11581 attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat
    11641 gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat
    11701 gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac
    11761 ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat
    11821 agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc
    11881 agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata
    11941 gataggcttt catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa
    12001 ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt
    12061 gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa actaacaagt
    12121 ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct
    12181 gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac
    12241 cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga
    12301 ctggacaata ataaaataac ccacattgat actaataata catccgatat tggaacttta
    12361 ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat
    12421 tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact
    12481 ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat
    12541 aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat acacacttac
    12601 aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJTOW010000105        12359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTOW010000105 DAJTOW010000000
VERSION     DAJTOW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.105"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO244_003215"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO244_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5664938.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO244_003216"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO244_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5664939.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO244_003217"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO244_003217"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5664940.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO244_003218"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO244_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5664941.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO244_003219"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO244_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5664942.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO244_003220"
     CDS             4025..4615
                     /locus_tag="OO244_003220"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5664943.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO244_003221"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO244_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5664944.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO244_003222"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO244_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5664945.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO244_003223"
     CDS             complement(6551..6658)
                     /locus_tag="OO244_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5664946.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO244_003224"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO244_003224"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5664947.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO244_003225"
     CDS             7648..7875
                     /locus_tag="OO244_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5664948.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO244_003226"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO244_003226"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5664949.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO244_003227"
     CDS             complement(9447..10703)
                     /locus_tag="OO244_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5664950.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO244_003228"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO244_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5664951.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO244_003229"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO244_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5664952.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12359)
                     /gene="torD"
                     /locus_tag="OO244_003230"
     CDS             complement(12282..>12359)
                     /gene="torD"
                     /locus_tag="OO244_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5664953.1"
                     /translation="YDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         2959 a         3141 c         3116 g         3143 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccacaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttat cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcata
//
LOCUS       DAJTOW010000106        12219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTOW010000106 DAJTOW010000000
VERSION     DAJTOW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.106"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..492)
                     /locus_tag="OO244_003231"
     CDS             complement(<1..492)
                     /locus_tag="OO244_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5664954.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO244_003232"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO244_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5664955.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO244_003233"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO244_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5664956.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO244_003234"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO244_003234"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5664957.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO244_003235"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO244_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5664958.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO244_003236"
     CDS             3646..4155
                     /locus_tag="OO244_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5664959.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO244_003237"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO244_003237"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5664960.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO244_003238"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO244_003238"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5664961.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO244_003239"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO244_003239"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5664962.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO244_003240"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO244_003240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5664963.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO244_003241"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO244_003241"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5664964.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO244_003242"
     CDS             complement(8797..9690)
                     /locus_tag="OO244_003242"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5664965.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO244_003243"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO244_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO244_003244"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO244_003244"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5664966.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINRISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="OO244_003245"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="OO244_003245"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5664967.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2967 a         3160 c         3116 g         2976 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctccattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgccg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacagg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJTOW010000107        12171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTOW010000107 DAJTOW010000000
VERSION     DAJTOW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.107"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            508..591
                     /locus_tag="OO244_003246"
     CDS             508..591
                     /locus_tag="OO244_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5664968.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(645..1997)
                     /gene="gorA"
                     /locus_tag="OO244_003247"
     CDS             complement(645..1997)
                     /gene="gorA"
                     /locus_tag="OO244_003247"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5664969.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2069..2911)
                     /gene="rlmJ"
                     /locus_tag="OO244_003248"
     CDS             complement(2069..2911)
                     /gene="rlmJ"
                     /locus_tag="OO244_003248"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5664970.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQVVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3114..5156
                     /gene="prlC"
                     /locus_tag="OO244_003249"
     CDS             3114..5156
                     /gene="prlC"
                     /locus_tag="OO244_003249"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5664971.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5164..5916
                     /gene="rsmJ"
                     /locus_tag="OO244_003250"
     CDS             5164..5916
                     /gene="rsmJ"
                     /locus_tag="OO244_003250"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5664972.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5965..7434)
                     /gene="dtpB"
                     /locus_tag="OO244_003251"
                     /pseudo
     CDS             complement(5965..7434)
                     /gene="dtpB"
                     /locus_tag="OO244_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7752..8186)
                     /gene="uspA"
                     /locus_tag="OO244_003252"
     CDS             complement(7752..8186)
                     /gene="uspA"
                     /locus_tag="OO244_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5664973.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8580..8915
                     /gene="uspB"
                     /locus_tag="OO244_003253"
     CDS             8580..8915
                     /gene="uspB"
                     /locus_tag="OO244_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5664974.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8986..10485)
                     /gene="pitA"
                     /locus_tag="OO244_003254"
     CDS             complement(8986..10485)
                     /gene="pitA"
                     /locus_tag="OO244_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5664975.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10717..11916
                     /locus_tag="OO244_003255"
     CDS             10717..11916
                     /locus_tag="OO244_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5664976.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         2991 a         3098 c         3249 g         2833 t
ORIGIN      
        1 gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt cagctggaga
       61 agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat cgggtcgctg
      121 ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc ggttttttta
      181 ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc taacgcatca
      241 atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc taattacact
      301 gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc
      361 aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagatt
      421 gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat
      481 tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg
      541 ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa
      601 cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg catggtcacg
      661 aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt tttggttgcg
      721 cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa gccaatgccg
      781 tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg gcacggctgg
      841 cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga
      901 tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga
      961 tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta
     1021 aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca
     1081 cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata tttatcgacg
     1141 acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg
     1201 gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc
     1261 agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggta
     1321 tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg
     1381 aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta
     1441 atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc
     1501 aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc tggaatatcc
     1561 gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg
     1621 ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta
     1681 tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg
     1741 gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata atcagggccg
     1801 tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag
     1861 ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca tttctggccg
     1921 tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa
     1981 tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag
     2041 acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt acggtggcgt
     2101 gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta ttcatctgct
     2161 gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat
     2221 cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca
     2281 ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac aatgcgtaag
     2341 tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccacc tgatagtcgg
     2401 ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt
     2461 tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg tctttctgaa
     2521 atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt
     2581 gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc aactggccgc
     2641 tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc aaatcgtcct
     2701 gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctac
     2761 ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct
     2821 ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc
     2881 cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatt
     2941 ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc
     3001 cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt
     3061 atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga
     3121 atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg
     3181 ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc
     3241 aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgttt
     3301 tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc
     3361 gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg gtagggcaac
     3421 atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac gccacgctga
     3481 acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc tctggcatag
     3541 gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt tctgaactgg
     3601 gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg
     3661 acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg
     3721 aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac ctgccggtaa
     3781 tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac agcacccgcg
     3841 cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg gaagagatcc
     3901 tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc tttaaatccc
     3961 ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc gatctggcaa
     4021 aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc gctaaagccg
     4081 aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacagaaac
     4141 agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa aacaaagcgg
     4201 ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag
     4261 atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa aataacgaac
     4321 tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga
     4381 tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt
     4441 atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc actcacgacg
     4501 aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg
     4561 aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc
     4621 agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactatg
     4681 aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc
     4741 aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc cgccttcatg
     4801 ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac
     4861 tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc agccatattt
     4921 tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta ctggcggcag
     4981 atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc
     5041 tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc
     5101 gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat
     5161 tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg
     5221 gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg
     5281 gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt
     5341 gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg
     5401 aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg
     5461 gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat
     5521 ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc
     5581 ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac ggcgctaacc
     5641 gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa
     5701 agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat
     5761 gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc
     5821 ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt gactaaaggg
     5881 catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt agcgataacg
     5941 ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag
     6001 ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc
     6061 aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc
     6121 ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat
     6181 cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag
     6241 ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga taaaccatgg
     6301 cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg tcaaaaagcc
     6361 cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt ctttgccttt
     6421 gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg
     6481 gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt
     6541 gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac
     6601 cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt
     6661 gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca gcaccagatt
     6721 ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca cgctgccaag
     6781 taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag aaccaatgtc
     6841 tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgccccgca
     6901 caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca gcgataacgc
     6961 tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgcgg
     7021 atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt
     7081 accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc
     7141 ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc gtttggtccc
     7201 caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa
     7261 agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac
     7321 cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat
     7381 catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat taatatttat
     7441 taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt
     7501 tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac tattacaaca
     7561 gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa ttttgtattg
     7621 gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta cgctgcatca
     7681 ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg
     7741 tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc aacgtgaacg
     7801 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     7861 caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg gcctaggtcg
     7921 ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag ctcggtcagc
     7981 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc
     8041 ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtag
     8101 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgact
     8161 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8221 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     8281 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     8341 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atattatatt
     8401 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
     8461 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
     8521 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
     8581 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
     8641 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
     8701 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
     8761 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
     8821 gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt ggtctggtgg
     8881 tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa taacgtataa
     8941 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     9001 ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa acacccaggc
     9061 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
     9121 cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat aactcgccag
     9181 gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     9241 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
     9301 acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag
     9361 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac caggcatctt
     9421 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
     9481 atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt
     9541 gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc
     9601 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg caggatgctg
     9661 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
     9721 ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca acataaccag
     9781 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
     9841 gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
     9901 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
     9961 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    10021 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    10081 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    10141 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    10201 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    10261 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    10321 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    10381 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    10441 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    10501 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    10561 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
    10621 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    10681 acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt
    10741 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt aggacgccgg
    10801 gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc tggcggtggg
    10861 cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat
    10921 ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct ggtcaataaa
    10981 cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
    11041 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt cagattgcgt
    11101 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct gaacggcatg
    11161 actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc
    11221 gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
    11281 gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
    11341 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg tgagaactta
    11401 ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag ctactggcaa
    11461 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    11521 gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt tcatctaccg
    11581 aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag
    11641 ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
    11701 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
    11761 ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg
    11821 atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    11881 gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc cgtcagccgt
    11941 ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt ctggtgttaa
    12001 ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat agtgtattta
    12061 ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa ggaaattatt
    12121 tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc c
//
LOCUS       DAJTOW010000108        12052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTOW010000108 DAJTOW010000000
VERSION     DAJTOW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.108"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO244_003256"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO244_003256"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO244_003257"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO244_003257"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5664977.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO244_003258"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO244_003258"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5664978.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO244_003259"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO244_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO244_003260"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO244_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5664979.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO244_003261"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO244_003261"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5664980.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO244_003262"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO244_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5664981.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO244_003263"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO244_003263"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5664982.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO244_003264"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO244_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5664983.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO244_003265"
     CDS             9553..10332
                     /locus_tag="OO244_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5664984.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO244_003266"
     CDS             10322..11131
                     /locus_tag="OO244_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5664985.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO244_003267"
     CDS             11218..11652
                     /locus_tag="OO244_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5664986.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12052
                     /locus_tag="OO244_003268"
     CDS             11649..>12052
                     /locus_tag="OO244_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5664987.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKG"
BASE COUNT         2877 a         3245 c         3076 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaacccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttacct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa gg
//
LOCUS       DAJTOW010000109        12042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTOW010000109 DAJTOW010000000
VERSION     DAJTOW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.109"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(45..806)
                     /gene="envY"
                     /locus_tag="OO244_003269"
     CDS             complement(45..806)
                     /gene="envY"
                     /locus_tag="OO244_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5664988.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(989..1879)
                     /locus_tag="OO244_003270"
     CDS             complement(989..1879)
                     /locus_tag="OO244_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5664989.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(1880..4848)
                     /gene="nfrA"
                     /locus_tag="OO244_003271"
                     /pseudo
     CDS             complement(1880..4848)
                     /gene="nfrA"
                     /locus_tag="OO244_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(4835..7072)
                     /gene="nrfB"
                     /locus_tag="OO244_003272"
     CDS             complement(4835..7072)
                     /gene="nrfB"
                     /locus_tag="OO244_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5664990.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7222..8664)
                     /gene="cusS"
                     /locus_tag="OO244_003273"
     CDS             complement(7222..8664)
                     /gene="cusS"
                     /locus_tag="OO244_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5664991.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(8654..9337)
                     /gene="cusR"
                     /locus_tag="OO244_003274"
     CDS             complement(8654..9337)
                     /gene="cusR"
                     /locus_tag="OO244_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5664992.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9494..10876
                     /gene="cusC"
                     /locus_tag="OO244_003275"
     CDS             9494..10876
                     /gene="cusC"
                     /locus_tag="OO244_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5664993.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            10900..11232
                     /gene="cusF"
                     /locus_tag="OO244_003276"
     CDS             10900..11232
                     /gene="cusF"
                     /locus_tag="OO244_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5664994.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            11248..11967
                     /locus_tag="OO244_003277"
                     /pseudo
     CDS             11248..11967
                     /locus_tag="OO244_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2891 a         3087 c         3151 g         2913 t
ORIGIN      
        1 ttcttcttca tttatgcccg tcttatccgt ttccgctttg cccttcacca catcactttt
       61 tgtcgctgtt tggcgagata attcaacggt gtcaggctgt aaaacgcctt aaaaacagag
      121 ataaagtacg acgtgctgct atagccacat aattgcgcca cctgagtgat atttttgtta
      181 tccatcaata acatctgtac ggcgtaacgc atacgacact ctgtgacaat ctggctatag
      241 ctggtatttt cgttttttaa tttctttttg agcaggctgg ggcttaaaca tagcgaactg
      301 gcgacaattc gcagattcca gtaatgctga atatcgcttt gaataatgcg gcagacgctg
      361 tcgcggacgc tgctgcgtaa gatatacatc agtagggcaa taaaccgcga ttgctcaaga
      421 aagttagaca atacggtaaa aagtaatgcg cgcgtcaact ccgcttcgca gggtttgctg
      481 gattgctgct tgctgtggtt ggtggctagc cggaacactt ccggtgtacg gctatgacaa
      541 gcgataaccg gggttgccag ccgcgaccag ggctttacac aggttaattt cttatttaaa
      601 aacaacagat aatcgttgat gatgttacgg ttgatgtggg tgattaatac gtgatttaac
      661 gttgagagtt caatgacgtt attgttgcac gcgaaggcgg ccaggtagtt tttcggaagg
      721 gtaaacgtag catggttatt gacgcttacc tctacctctt tttcggtcag gatcaccacg
      781 caaggttcac tgctgctcaa ttgcatttcg cactcctcag atatcagaaa ctccgctcaa
      841 aggatctatg cttcctgcat gagtgatcgg cccgttcgcc gataacgatc ttctttcttt
      901 agcatgcttt ttagcaatta atcttgatgg aattctgacg agagcgaaag aggtaagcca
      961 ggtcgtaccc gacttacctg gaggggattt aatactcgag aatgccgtgc gcgacgggca
     1021 aatagcgcag agagaaatag agcatgtctt taccgcaaga ggaacgtttc gctaacagcg
     1081 aggcaatttc tgcgtccggt ttcggtttcg ctgtaaaggt tttgcctttg ccggcgacaa
     1141 acagttcata aacaatgccg cttgctgggg cggagctttg gcaatcggcg ctggcctgta
     1201 gataacgttc acgctgttca gcggtaagtt tatccacgcc gctgccatcc tgtacccaga
     1261 cattaactcc ggttgcttta acttgttcca gcagttggcg atagccatcg ggcgacatgt
     1321 ttccggcgaa aaaactactg atataaaccg gttttgctga aacatcgcta atcagccgct
     1381 gcgcgttgtt taaccatgtt agcaaaggtt gacgggcggc ttcgctgcgc cagttcaggt
     1441 cgtcaatttc cgcgctgatg taccagccat ccggcgttat gccaggcgcg gcgctccata
     1501 atctggcttg ctggagatcg gcagccagca ggcgattaag atagctttcc agcgctgcag
     1561 acgactgttt ctggtgcata aaaaattccg gatcggcgtt cagcccgaca atcagcttca
     1621 ggccagtctg ttgcgcagct gcggcacgct taaacaataa cgtgcgctgt tctggctggg
     1681 taaatgcatc gccgtaacgg gtccattgca aaaccagggt atcgaagcct tgaaaacgta
     1741 actgactcat cagcccctgc cactgggtat cggtaacctg actatctcgg ttttgtggtt
     1801 gccagataat acctttcatc gcaaaggaaa aagggctgac caaaagcagt gtcagcaata
     1861 cgaaaatgaa cttacgcatt taccagtgca ctccaatggt gagaaacgcg ttgttgcgct
     1921 ctccgttacg ttgattaatc gccttaaagg tatgttgata ctcgacgccg agactgactt
     1981 tgtgcggcca ggcgtcgtag tgcgtctcgc cggtccaaat attccagcgg accccgacac
     2041 cgccaagctg cgcgccctga gtgcctttat cacgatagcc gttgtcctga acgtgagcgt
     2101 aaggctcaat agtctgtccg ttagctacct tctgatgcca gctgacgcga taatctgccg
     2161 tccacgcctg aatatcctgg cggatatatt gcgccgcatc gaggtacagg ttttgggcaa
     2221 accagcctga accgttcggg tgccattcgt cgctgtattt gccgccatta aagaatgagg
     2281 cgctggcgcg cagcatggta tcggatgcgc cattttgccc gttcagcggc aactgctgtt
     2341 cgacggcgag gaaaaagatc tgatcgcgca gcggcttcca gcgcagaccg gtgccggaca
     2401 tcggattttt caccggcatc atcaccccgt tttctccggt atcggcaaag acgcggctat
     2461 aaactgagag caggtcgcct tccagcagca tattgcgtcc gatgcggtac tcggcttcca
     2521 gttgtccgta gctacgatag cttttcccag gcgctgcacc gccgacatta ttgttagcgg
     2581 tactcattgc gccggaacgc aagccgatgg aagaatcgaa actgaacgtc cagcggcgac
     2641 cgacctcctc atgcaaacgg cggaaattga agcgttgctg attttgctct ggggtcagtg
     2701 gggttatgcc tgattatcaa tgtcatcaat cacgagccgg gcgtagtgct gcgtcgcagg
     2761 catgtcatcc agacgctggt tcacgtaggc cagttgtcgg atcagtgccg gatcgtccgg
     2821 aagcccttta tgcgcctgtt cgagcatttc ccgcgactgt gcgatatcac cgctatccca
     2881 caaggcgtaa ccaagcgctg cctgggtgtt gctattattt ggttccagtt ccagcgcggc
     2941 gcgcaaatca ctcaccgcgg ccggaacatt atgacgttgg cgataaattg tcgcccgcgc
     3001 aacgtaagag ttggcagaag gggcaatatt gattgagcgc gtgagatcgt tcagtgcgag
     3061 ttctggctga gctgggatat aacgtcgcgc atgcagccac cagtattgag cattgtttcc
     3121 cagctcacgt tgttctgcct gttgaagcca gcgatcgcga gctgcgccat ttcccgctgc
     3181 ctgagcggtg ttggcggcgg cgagcagatc atcattgctc atgtcgtgaa gactgatttt
     3241 ctgccaggcc gccagtgcgg tggcgtagtc ctcaacctga tacgcctgat aggctaccgc
     3301 acgatgttgc caggcgctcg gttgtcgttg ttcggcctga agccatgcat acaacgccac
     3361 accgggtagc gtgtcccgat aacactttgc cagacggttc caggcggcag catcgtagga
     3421 aggcgacata tcgcccagca agcgaactat tgccgggcaa ttatctgcaa tacccggcaa
     3481 ctgactttgc cactgacgtt gctccgccag cggtaagggt ttcgataaaa tcgccacctt
     3541 cgccggcgtt gctaggtaag gatgactttc cagcagagac gccagtcgcg ccattaaagt
     3601 ctggctgaca cgcgcatcgc cctggaaagg atagcgttgc agcaataaat cggcagcttc
     3661 gcgtgactgc tcgttctgca tcagttgcca ggtcagttga tccaggcggg gaagatttgt
     3721 cggttcttgc tgatacagca atcgtgccag acgcagagat tcagccttgt tacgggtcgc
     3781 cacgctgaca gcataacgct cctcaagcat ttcattggcg gggagggtgg cgagcagttt
     3841 ttgcgctgcg tcgtactgac cttcttttaa cagcaccggt agcgtcgcgc caacaacata
     3901 ctggcggtta tcggcaaact gcaccgtata attcgccaat gcctgaacgg ggttggcgct
     3961 gtatttagat aacagataga gccaactttt ctcttgtgcg cccgtggtaa acagcggctt
     4021 attttcaatg agataatgct ggaggcgtgc tttttcgcca cgataagcca gcgcggtcgc
     4081 gtaagtaata tatgactgag gatcggtgaa gatcccctgt gattgcagtg ccaggatccg
     4141 atcgtccagc tgcccggcaa gaagcacgtc aaaccactga cggcgttctg ccgcgcttaa
     4201 tgtgttctgc tggcgtgctt cattgtatag cgtatctgcc tgggatcatt gtttcaggta
     4261 gattgcccgt tgcagcagat cggttcgcag cgtttttcct tccggcgatg cagcaaacgt
     4321 cgcatcgttc agttgcgctc tggcgacagg taattgtgcc agccgcaggg cattctgccc
     4381 gacttcactg cgacaacgca gggtcggcgc agcgtcgccc gctttttgct gggcaagcag
     4441 ttcttcaaca gtcgtaacgc ttttcacttc aactggaata gccgccagac tgcgctcaag
     4501 tcgggcatct cctgggtgac gtttcagttg atcctcaagc aacagccgcg cccggtcatc
     4561 atgaccaaaa tgacgatagg cttccgcaag gtataaagtc agcggaatat tatccggcac
     4621 ctgttggtgt atatattcaa attcgcggat ggcggttgct tcgtcgttat ttttctgtgc
     4681 cttcagcgcc ttatcgagac ggggataaat aacaaaatgg cgataatcgc tcagccccag
     4741 ctcttctgcg ctggtgccga tattgtctgc gagtgcgctg gtactcaata aagacgtcag
     4801 cagtaaacca gaccatccga tgacgcgatt aaggttattc tccttcattt tcggactcca
     4861 gttgcgcaac ctgttctgtg tttaaacctg ctttgagtaa tagtgattgc atcgaaactt
     4921 gtaattcgcg ttgaattgtc aggacgcgat ccaacgtttc ctggctgata acgccttcgg
     4981 tgaccaaaaa cttgccgagc ggcagagaac tgcgttcatg gcgcaataac aacacgttaa
     5041 ttgctgaacg attaatatga ccgagcgtgg tcagtatttc ggcgaacagg aactgatgcg
     5101 gcacatattg ccgccagatt tcaccggtct gctgttccgt gagccactga tgctgaaccg
     5161 cattgtacaa cattgcccgc ggatcgtgac cgcgtcggcg tgcataccag tggcgtaacc
     5221 ctgtgacaat ttgtccccgc agaacaatga cgtaacgcac tttgcgtccg actttacgcg
     5281 tcagggccgc cagcgaaacc gggtcaatac catcttcact gccgacaatt aactcatcat
     5341 tttccagacg cagcggcagt actgcataat gcagcgctac ggaggctggc atttcggcaa
     5401 tcagcgagga cgggatctgc caggcatcga tggattccca cgccacgccg ttttgctctg
     5461 ccagcgcctg tgccagctgc tcggcgctaa tcagcccctg catcagcatt gaaccgccca
     5521 ggcgtagacc ttcgacgcga ttacgcagtg ctgtatcgag ttgttcttca gtgatgacct
     5581 gattttccag cagaatttga cctaacgggc gcaacgagcg ggtatcgcca gtgacgctgg
     5641 ggaagtcatg cgttgtttta tcccacgcga cgcgacgcgg atcgccgtgc tgaagaacct
     5701 gtttcagcac gcgccagtta gccataaagt taatcaggtt gccccagaaa agacgcagga
     5761 cggaaagcag gccctgcgtg aggccgtagt agccagtgac gaaaatcact cgctgcacga
     5821 tgcggttaac catcaagcca aagtttagcc acagcagggt cattaaccat gcgctgccgc
     5881 taaaaataga aaggaaatgc caggcatcgg gccacaaact ttcatacgcc agcaacagca
     5941 aaagctggat catcaccagc atcgcgagga agctgacaaa gttactgatt gcccctttgc
     6001 ggtcgcgcca gagaaagtag ttcagcgtca ggctggaggt ccatttatgg gttttaaagc
     6061 cctggaaaac aatgccgatg atccagcgag atttttgtcg aaccgcagtc gaaaaggtat
     6121 cggggaaata ttcgcgcacg cagatcatgt ttgacgtccg tgcgtgctgt aaaaatttac
     6181 gctgctcgcg ttctttggct tcgtccacca ccggaaaacg gacaaaaatt tccgtcatac
     6241 ctttttcttt caggcggaag ccaatgtcgt aatcttcagt aagactctgc acgtcgaaag
     6301 caataccgtt accgtcagct aacagtgcgg tcacggcgcg gcggctgaaa caggtgccga
     6361 cgcctgcgct gggcacttgt ccggcgaggg cttcacgcac cggaacatct ttgccatgca
     6421 gttctgaaaa ctcatcaatg taagtcatgc tggtgaagtg cgtccattcg cgttcaaacg
     6481 gatacactgg gatctgaatc agatctttac gctcgaccag atagttgaac agacgcaatt
     6541 ccatcggtga aatcacatct tcggcgtcat gcagaataaa accagcaaaa gcgaaattgg
     6601 cgctacgttc aaactgggtg atggcgtcca gcacgttgtt caggcagtcg gctttgctgg
     6661 tgggaccagg acgcgcgcag actaccttat gcacgttcgg aaagcgagcg cacacttcgt
     6721 caacatcacg ttgagtatcg gggtcgttgg ggtaggtgcc aacaaagata tgatagtttt
     6781 cgtagtcgag cgtggtcgcc gccagctcgg ccatattgcc gatgacgccc gtttcattcc
     6841 acgccggaac cataatcgct aacggttttt catctggttt atacagttcg cggtaactca
     6901 ttcgcgggta gcggcgataa acactcaact tgcgtttaat gcggcgtacc cagtagacga
     6961 catcaataaa aaaatcgtcc agcccgctga tgaacatgat gaccgctaac gttatcgcga
     7021 ttacttttaa gccgtagagc caggtagcaa aaacatcaag aagccagtcc acacaaaaac
     7081 cttacattaa cgctggttat gtttagggtg gcgtatatta aggtttttta tgaattgtga
     7141 cagcttttta ccattaatat gtatgactat tgcggcacgt tatttttaca ctggttataa
     7201 aagttgccgt ttgctgaata attaagcggg taatgtgata acaaaccttg tcccccgcgc
     7261 atctgacgtt accgcaaccg tgcctttatg cgcgacaaca atcgatttca ctatcgccag
     7321 cccaataccg ctaccttcac cttttcgctg gcgggagggg tcaacgcgat agaaacggtc
     7381 aaacaatcgc ggtaagtgct cgggcgcaat gggcgtaccg gggttttcga cggaaacttg
     7441 cacctggtga tcgaccgtct gacagcgtac tacaattgtc tctctggttg gcgtataacg
     7501 aagggcatta gaaagcaggt tgcttagcgc ccgacgcagc atcaacggat cgcccgcaac
     7561 ctgacgtttg tcgccaacaa accgcaactc cacgccgcga tcttccgcta gcgcctcgaa
     7621 aaaatcgaac actttgccga cttcatccgc caggttgagc attttctttt cggggattag
     7681 ctggttgtta tcggcctgag cgagaaacag catatcgctg accattttcg ccattcgcgt
     7741 cagctcttcg agattagagt agagcacatc ttccagctcc ttctggctgc gcgactggct
     7801 gagggcgatt tccgtttgcg taatgagatt agtaattggt gtgcgaattt cgtgggcgat
     7861 atccgctgag aaattggact ggcgagtaaa gacatcctca atacgctcga tcatatggtt
     7921 gaacgacagt accagctgtt ccagctcaat cggcacggtc tgcgggtcaa ggcgaacgtc
     7981 gagatctttc gaggtgatat tctggatttg acggctgacg ctgcggatcg gcgcgtgacc
     8041 tttatgtacc gccaacagca cgataaagac gatcaggatg ctgattaccg atgcggtcat
     8101 aataagttta ttcatcaaat catttatgta atgaagatga aaatcgatcg acagcgcgat
     8161 gtagagcgta taaatcggtt tgccgtccac caacgggcca accggcaagt taatcatccg
     8221 ccagttgctg tgttccatat gcccgtgacc gtggcctggc atcataatcg tcgggccgga
     8281 aaggagatac acctcgccac cccgcgcgtc tttatcgggt atggcatcac gtgcaaactc
     8341 gcggatatca ggcgcaccgg gggagtgata caccgttttg ccgtggctat ctgccaggga
     8401 aatcaacacg ttggaataac cactgacgat atcttccagc gtcattaagc gtcgggcttg
     8461 cgtttcgtca gggtgattta gtacccgttc aagggtggcg ctaatctctt ttaaatcatt
     8521 aatgtcctgc tcggcaaaat gcacttttac tgagtggatt atgatccatg caaaggcgaa
     8581 aaacgccgcg atggtggcca ggctgataaa aaaggtcagg cgggttgcca gcgaaaacgg
     8641 gcgctgaaat ggcttactga ccatccggca cctcaagcat gtaacccacg ccgcgcacgg
     8701 tttgaatcag cttcggctca aagtcgttgt cgattttgcc gcgtagcagc ttcaccgcca
     8761 catcaatggc gttagtgtcg ctgtcgaaat tcatgtccca tacctgcgag gcgataagcg
     8821 agcggggcag cacttcgccc tgatggcgga gaaaaaactc cagcagagta aattctttac
     8881 tggtcagagt gatgcgcgtg ccgctgcggg tgactttgcg gctgacgaga tcgaccatca
     8941 gatcggcaac ctgaaactga ctttcgataa tcaccgccgc cccgcgccgc aggagagtgc
     9001 gcacccgcgc cagcagttca gcaaaagcga acggtttcac caggtagtca tctgccccca
     9061 actccagccc cttgacgcga tgttcaatgg tgccaagcgc ggtaagcaac agaatcggca
     9121 tccctttatt ggcggagcgc aacatgcgca cgatatccca gccgttcacg tctggcagca
     9181 taatatcgag gattatcagg tcataatcac cggtcatcgc cagatggtag ccattcagcc
     9241 cgttgtcggc caaatcgacc acaaaaccgg cttcggttaa ccctttggtc aagtattctc
     9301 cggttttctt ttcatcttcg acaatcaaca gtttcatatt tcctccgcat gttgcccggg
     9361 caattctaga gtagcgggat cagatggcaa tcgcttattg gcaaaatgac aattttgtca
     9421 ttttcctgtc accggaaaat cagagcctgg cgagtaaagt tggcgacata aaatcaccag
     9481 aaattatgag cctatgtctc cttgtaaact tctgccattt tgtgtggccc ttgcgctaac
     9541 tggttgttca ctggcaccgg attatcagcg tccggtaatg cccgtaccgc agcagttctc
     9601 actcagccag aacggcctgg ttaacgcagc ggataactac cagaacgtgg gctggcgcac
     9661 cttttttgtt gataatcagg tgaagacgct gattagcgag gcgctggtga ataaccggga
     9721 tttgcgcatg gcgacgctga aagtgcagga ggcacgggcg caatatcgtc tgaccgatgc
     9781 cgaccgctac ccacagctca atggcgaggg cagcggcagc tggagcggca atcttaaagg
     9841 cgattcagcc acgacgcggg agttctcgac tggccttaac gccagctttg atctcgattt
     9901 tttcggtcgc ttaaagaaca tgagcgaagc cgagcgccaa aattatttag ccactgagga
     9961 agcccagcgc gcggtgcata ttttgctggt ttctaatgtc gcgcaaagct atttcaatca
    10021 gcagctggcg tatgcgcaat tgcaaatagc cgaagaaacg ctgcgtaatt atcagcagtc
    10081 atatgcgttt gtcgaaaaac aactgttgac cggtagcagc aatgttctgg cgctggaaca
    10141 ggctcgcggg gtgatagaaa gtacccgcag tgacatcgct aaacgtcagg gggaactggc
    10201 gcaggcgaat aatgcattgc aactgttatt gggaagctac ggcaagctac cgcaagcgca
    10261 gacagtaaac agcggcagcc tgcaaagcgt taaattaccg gcgggcttgt cgtcgcaaat
    10321 cttattgcag cgcccggata ttatggaagc tgaacacgcg ttaatggcgg ctaatgctaa
    10381 tattggtgct gcgcgtgcgg catttttccc gtctatcagc ctgaccagcg gaatatcaac
    10441 cgccagcagc gatctgtcgt cattatttaa cgctagcagc gggatgtgga attttattcc
    10501 taaaattgag atccccattt ttaatgccgg acgcaaccag gccaatctgg atatcgccga
    10561 aattcgccag cagcagtcgg tggtgaatta tgaacagaaa atccagaacg cctttaaaga
    10621 ggtggcagat gcgctggcat tacgtcaaag cctggacgat caaatcagcg cccagcagcg
    10681 ttatctggcg tcgctgcaaa ttaccctgca acgggcgcgg gcattatatc agcacggcgc
    10741 ggtaagttat ctggaagtgc tggatgccga gcgttcttta tttgcaaccc gacaaacttt
    10801 acttgacctg aattatgccc gtcaggttaa cgaaatttat ttgtataccg ccttaggggg
    10861 cggttggcag caataatttt taactccagg agagaataaa tgaaaaaagc actgcaagtc
    10921 gcaatgttca gtctgtttac cgttattggc tttaatgccc aggctaacga acatcatcat
    10981 gaaaccatga gcgaagcaca accacaggtc attagtgcca ccggtgtggt aaaaggtgtt
    11041 gatctggaaa gcaaaaaaat caccatccat cacgatccga ttgctgccgt caactggccg
    11101 gagatgacca tgcgctttac catcaccccg cagacgaaaa tgagtgaaat taaaaccggc
    11161 gacaaagtgg cgtttaattt tgtccagcag ggcaaccttt ctttattaca ggatattaaa
    11221 gtcagccagt aacccaggtt taatgagatg aaaaaaatcg cgcttattat cggcagcatg
    11281 atcgcgggcg gtattatttc agcggcaggt tttacctggt ttgaccaggc ggaaccgcac
    11341 gcagaaaaaa tgccgaccgc agaacgtaaa atcttattct ggtacgaccc gatgtatccc
    11401 aatacgcggt tcgataaacc aggtaaatcg ccgtttatgg atatggatct ggtgccgaaa
    11461 tatgccgatg aagagagttc tgcgtctggt gtgcgcattg acccgactca gacgcagaat
    11521 ctgggggtga aaacggctac cgtcacgcgc gggccgctga cttttgccca gagtttcccg
    11581 acgaatgtca gttacaacga gtatcagtat gccattgtgc aggctcgcgc tgccgggttt
    11641 atcgacaagg tgtatccgct taccgtgggc gataaagtac aaaagggcac accgcttctc
    11701 gacctgacca ttcccgactg ggtggaagcg cagagtgagt atttactgtt gcgcgaaacc
    11761 ggcggtacgg cgacccagac tgaaggcatt cttgagcggc tgcgactggc gggaatgccg
    11821 gaggcggata ttcgccacct gatcgccacg caaaaaatcc agactcgctt tacgctcaaa
    11881 gcgcccattg atggcgtgat caccgcgttt gatctgcgcg cgggaatgaa tatcgccaaa
    11941 gataacgtgg tcgcgaaaga ggaaggtagg ttgcttatct gtgtctgtca ttgtctagca
    12001 ttgtccactg tagttaatta actgtctgtt ataactcgaa aa
//
LOCUS       DAJTOW010000110        11925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTOW010000110 DAJTOW010000000
VERSION     DAJTOW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.110"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..2342)
                     /locus_tag="OO244_003278"
     CDS             complement(<1..2342)
                     /locus_tag="OO244_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5664995.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPSETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO244_003279"
     CDS             complement(2276..2899)
                     /locus_tag="OO244_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5664996.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO244_003280"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO244_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO244_003281"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO244_003281"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5664997.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO244_003282"
     CDS             7507..8229
                     /locus_tag="OO244_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5664998.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO244_003283"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO244_003283"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5664999.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OO244_003284"
     CDS             complement(10301..11896)
                     /locus_tag="OO244_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5665000.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2945 g         2916 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgctt ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcgg
//
LOCUS       DAJTOW010000111        11840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTOW010000111 DAJTOW010000000
VERSION     DAJTOW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.111"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(186..830)
                     /gene="cheZ"
                     /locus_tag="OO244_003285"
     CDS             complement(186..830)
                     /gene="cheZ"
                     /locus_tag="OO244_003285"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5665001.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(841..1230)
                     /gene="cheY"
                     /locus_tag="OO244_003286"
     CDS             complement(841..1230)
                     /gene="cheY"
                     /locus_tag="OO244_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5665002.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1245..2294)
                     /gene="cheB"
                     /locus_tag="OO244_003287"
     CDS             complement(1245..2294)
                     /gene="cheB"
                     /locus_tag="OO244_003287"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5665003.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2297..3157)
                     /gene="cheR"
                     /locus_tag="OO244_003288"
     CDS             complement(2297..3157)
                     /gene="cheR"
                     /locus_tag="OO244_003288"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5665004.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3176..4777)
                     /gene="tap"
                     /locus_tag="OO244_003289"
     CDS             complement(3176..4777)
                     /gene="tap"
                     /locus_tag="OO244_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5665005.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4823..6484)
                     /gene="tar"
                     /locus_tag="OO244_003290"
     CDS             complement(4823..6484)
                     /gene="tar"
                     /locus_tag="OO244_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5665006.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6629..7132)
                     /gene="cheW"
                     /locus_tag="OO244_003291"
     CDS             complement(6629..7132)
                     /gene="cheW"
                     /locus_tag="OO244_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5665007.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7153..9117)
                     /gene="cheA"
                     /locus_tag="OO244_003292"
     CDS             complement(7153..9117)
                     /gene="cheA"
                     /locus_tag="OO244_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5665008.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9122..10048)
                     /gene="motB"
                     /locus_tag="OO244_003293"
     CDS             complement(9122..10048)
                     /gene="motB"
                     /locus_tag="OO244_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5665009.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10045..10932)
                     /gene="motA"
                     /locus_tag="OO244_003294"
     CDS             complement(10045..10932)
                     /gene="motA"
                     /locus_tag="OO244_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5665010.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11059..11637)
                     /gene="flhC"
                     /locus_tag="OO244_003295"
     CDS             complement(11059..11637)
                     /gene="flhC"
                     /locus_tag="OO244_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5665011.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11640..>11840)
                     /locus_tag="OO244_003296"
     CDS             complement(11640..>11840)
                     /locus_tag="OO244_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5665012.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2697 a         3329 c         2882 g         2932 t
ORIGIN      
        1 agtcatcgtc attttctcgt tcacaattta atgcattgat aaatttactg gcgcaacaat
       61 cacgcttcgc tgatctaaaa ttatcaaact acaaataacc atcctccacg tactccctga
      121 acgatacccg ccaccctgct atcggcttgg catcatcgcg caggccgaag ttcaggcaat
      181 acaaatcaaa atccaagact atccaacaaa tcgtccacct gatcctgact ggctaccacg
      241 ccggctttgc tggtatcgac ctgaggtcca ttaagcaaac tctggttttc acgttttgga
      301 cgcgactcct gttccgggat gttttccaac agcaccatca gcaactggcg ttcgatctcc
      361 tgaatgacat ccatcatccg cttaataacc tgcccggtga gatcctgaaa atcctgcgcc
      421 atcatgattt ccagcagttg cgcgttagta aagctggtat gcgcgggtac atctgccaga
      481 aattgtcgtg tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg atcggcaaac
      541 cagtcatccc aacgttgggt taacgctttt gctgatttct ccatttgatc ctgatgcggt
      601 tgtgacgcct caacactgtt cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca
      661 tagtacaaac gatcgcgcgc atcggggatg gcttccgccg cttcggcaat ggcctgaacc
      721 agccccagtt cccgcaaact gtcgcgcagc atatgcgtca ggctgccgat gcgcgcaatg
      781 atatcgccag ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc
      841 tcacatgccc agtttctcaa agattttgtt gagtttttcc tccagcgtcg cggcggtaaa
      901 tggcttcacc acatagccac tggcccccgc ttgcgccgca gcaatgatgt tctctttctt
      961 cgcttctgca gtcaccatta acactggcaa tgccgacatc gcgccatccg cacgaattgt
     1021 tttcagcaat tccaggccat ccatattggg catgttccag tcggagataa caaatccata
     1081 accgcctgcc tgcaacttat tgagagcgtc gacaccatct tccgcttcct caacattatt
     1141 gaatcccagc tctttcagca ggttacgcac tatgcgtcgc atggtggaaa agtcatccac
     1201 aaccaaaaat ttaagttctt tatccgccat ttcacactcc tgatttaaat acgtatcgcc
     1261 tgtccggcac taattgttgc caacatttgc tggcttacct ggctaagatc gaccacttcg
     1321 cagacaccac ccatattgat ggcctcgcgc ggcatgccga acaccacgca acttgcttcg
     1381 ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca acattcccgc cgcgccgtcg
     1441 ttgcccatac cggtcaggat caccccaacc gcattacgcc ccgcctgttt ggcgacagaa
     1501 tggaacaaca catctaccga aggccgatga cggttaaccg ccgggccgtc gtgaattttg
     1561 atttgataat ttgcgccact acgcgccagt tccatatgcc gatcgcccgg cgcaatatag
     1621 gcatgccccg gcaagacacg ttctccgtct tcggcttctt taaccccgat ctggcaaagc
     1681 ttattaagtc tgtcggcaaa agagcgggtg aaaccgggcg gcatatgttg ggtaattaac
     1741 aatgccgggc tggaaagcgg caacggttgc agtacgtgac gaattgcctc agttccaccc
     1801 gttgaagcac caatcgcaat cagtttttca gaactcaaca acggccctgc cttcagcgtt
     1861 gtcggtgctg acaatggctt atgtgctgca aggctcgcct ttgctgccgt acgcaccttt
     1921 tcagcaatca tttcgctata cgccagcatt ccttcgcgaa tacccagttg cggtttggtg
     1981 acaaaatcta ttgcccccag ctccagcgcg cgcagcgtga cttctgatcc tttgccggtc
     2041 aaggaagaaa ccatcacaac gggcattgga cgcaaacgca ttaatttttc gaggaaatcc
     2101 agtccgtcca tccgcggcat ttcaacatcc agcgtcagca catcgggatt gaatttctta
     2161 atcaagtcac gcgcaaccag cggatcaggc gcggtcgcca ccatttccat gtcgctatgg
     2221 ctgttgatga tttctgtcat gatctggcgc atcagtgccg aatcatcgac agataacacc
     2281 ctgattttgc tcatcgttaa tccttactta gcgcatacac cgtctgacca cgcagcgtga
     2341 agcggcgctc gaggtggcta aagttttcag agtgacccgc aaacagcaat ccgtcgggtt
     2401 taaggagcgg aacaaagcgg cgcaaaatct cctgctgggt agtttgatcg aagtagatca
     2461 tgacgttacg acagaagatc gcatcaaacg gccccggcac ggtgtactgt ttcgccagta
     2521 aattcagcgg ggcaaaatca acatagttcg ccagctcctg acgcacgcgt accagccctt
     2581 catgcggtcc cgtccctcgc atgaaatacc gttgcagttg ctgcggcgtc aggtttttca
     2641 actcttcatg gcgatagata ccgcttctgg ctttttccag cacttcggtg tcgatatcac
     2701 tggcaaacac tttccagcgt ccgggcacgg tgcccaatgt gtcagccaga gtcatcgcaa
     2761 tgctgtacgg ctcttcgccg gtcgaagccg ccgcgctcca tacgcgatcc tcgccagaac
     2821 ggcgacgtgc gtgatccgcg agcagaggga aatgatgagc ctcacggaaa aatgccgtca
     2881 gattcgtggt cagcgaattg ataaacgcct gccactcacc gctgtgctga ttagattcca
     2941 gcaagttcag ataatgaccg aaatccgtca gtcccagaga acgcaaacga cgaaccagtc
     3001 ggttgtaaac catgtcgcgt ttatggtcag ccagaacgat cccggctcgt tgatagatca
     3061 attgacttat ccgccgaaaa tgcgcgtcgg aaagcgccag gcgctcggtc atctgtaaca
     3121 ataaagacgt ttgcccacag ggcagagatg aagtcatagc gccttctcaa tcacttcagg
     3181 ataccactgg cgcaatttgt aactgcgccg actcatgtcg tgctacttca tgttcttcaa
     3241 gggtaaatac cgccacacgc gacgaaagat ggtcggcctg attcgccagt tgttccgttg
     3301 ccaccgccgc ttcttctacc agcgaggagt tctgctgagt cacctgatcc atctggctga
     3361 cagcctgtgc aacctgctca atcccccgcc gttgttcttc cgacgccgaa gcaatttctc
     3421 ccataatgtc gttcacgcgg gtcaccgaac tgacaatatc ggtcatggtc gcggcggcgt
     3481 tattcaccag tttcgaaccc tgctggacac gattgactga ctcttcgatc agccctttga
     3541 tctcttttgc tgcctgcgcg ctacggctgg caagattgcg cacttcacct gccactaccg
     3601 caaaaccacg cccctgctct ccggcgcgag ccgcttccac tgccgcattc agggccagaa
     3661 tattggtctg gaaagcaatt ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc
     3721 tggtggcgat ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg
     3781 ccgttgtcgc ggcatttttt gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc
     3841 ctaccgtggc ggttaattgc tccatactgg cggccgtttg tgccagcgat gccgcctgct
     3901 gttcggtacg acttgagaga tcgttattgc ctgcgacaat ctccgcgatc ccaatgtgca
     3961 tctcatggct tcccttacgc acatcactca ccgtcccacg caaagcctgc tgcatggtct
     4021 tcaggctggc aaaaatggcg gtgatctcat tacgaccata taccgcaatc ggacgcgcca
     4081 gattacccgc agcaatgctg tcaaaatggc taccgataat ggccagtggt tgaacaatca
     4141 tcttgcgcgt ccaccacagc gcactgctga tgtagatcgc cgcaacaata atcatgctga
     4201 taaacaccag cgccgaaatc tgatagttac gctggctttg cgcactggcg gcttccagca
     4261 catggttgat ctccagctgc caggcctcaa agttaacgtc aaacgcattc tgtgatccct
     4321 gcaccggcgc agtgaggaaa tccgaaagtt gattactttc aagccaggtc gcctgatgtt
     4381 cgagatcgtt gtgccagcgg gcaaaacttt tctccgtctc tttttgcaat gccctgacgt
     4441 gctcgttgcc cgcagtcatc gccataaaac ttttaaacag cgtggtggat tgcgtcagac
     4501 tggcgcgcgc cgtcgtcatc aacgttttaa tgtcatccgc cggatagcta agcgctgtca
     4561 gagtgcctgc tttgttcagc gcggtactgg cctgtaacat taccgcccga gtttgcgcta
     4621 atgccgcacg ttgctgattg ctctgctcgc cgtgattcag tcgttgcaaa tcgtcgcgaa
     4681 atgcccaaaa agacatgccg ttactgccaa tctgcaagat cccgcagaga atcaaaatta
     4741 aaaacagcgt ggtcgaaatt cgaatacgat taaacatcaa cgctccccat caggcggcaa
     4801 tgaccgcgtt agtaaaaact cgtcaaaatg tttcccagtt tggatcttgt tcagtaattc
     4861 gcagtcgtgg ctgtgccggt ggttgctcac tggcaggacg ggatggtgtt tgcggtttat
     4921 tggtgagtgg gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa cgactcgcct
     4981 gttcttccag cgcagctgcg gcggcagctg attcctgcac cagcgatgcg ttctgttgcg
     5041 tgacgcgatc catttccgaa accgccaatg cgacttgatc gatgccacgg ctctgttcat
     5101 ccgatgccga tgcaatctcg cccataatgt cagtcacgcg agtgacagca ttgacgatat
     5161 tgttcattgt ttccccggcg ctttcgacca gcaccgaacc ggtatcaatg cgcgagacgg
     5221 agtcttcaat gagggctttg atctcttttg ccgccagggc gctgcgactg gcaagattac
     5281 gcacttcacc cgccaccacg gcaaaaccac ggccctgttc acccgcacgc gcggcttcaa
     5341 ccgcggcatt cagcgcgagg atattagtct ggaaggcaat accgtcgata acgctgataa
     5401 tgtcggcaat tttcttcgaa ctatcggcga tctcatgcat cgttttcact acgccatcca
     5461 ccactttgcc gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc gaggcctggc
     5521 gggcgttatc ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg gcggcagttt
     5581 cttccagcgc ggatgcctgc tgttcagtac gggaggaaag atcggtgttg cccgccgcaa
     5641 tttcacgggt accggcatag atggcatctg aaccttcgcg gacatgagtg acggtgtcag
     5701 tcaaagagcg ttgcatatgt gaaacgctct gcgccaggtc acccatttca ctgcgcccgt
     5761 caatggtcag ggtattcgcc aggttaccac cggcgatttc gcaaatgtga gcaataattt
     5821 ttgccagcgg agtaagcaac atacggcgaa tgccgtacca cgccaccagc agaatcaata
     5881 ccaccaccag cgcgataacc gccagttgcc actgggcaaa tcggtaatca tctgcgttgt
     5941 cagtgacgat atcgcgatac agtttttcac tgctgagggc gtactgagca aacgcttcgc
     6001 ccattgcatt ttgcattccc tgggttggct gagcgaaata agctccagta ttgccataat
     6061 caagataatc aatcagttca gttaacgctg tgtaatagtt tttatatttt tcatcaaaat
     6121 tacggctggt agcgaccatt tcaggtaacg gtgccatgct tttgaatttt ttataatgcg
     6181 tcgcggcctg ggccaatgtt ttcctggcgc tatcgagcaa ttcaactttg gcgttacttt
     6241 gttgattgga ggaatccatc atcatccgca ccgctgaacg actcaggtta atgcgcgttt
     6301 gcagcattaa atcccaggtt gacgtcagct cgccctgctg ttcccgtaat tgattggaaa
     6361 ccacaaagct cttctggcta tggtgaaggg aagaaaaaaa caaactgccg gaaataagct
     6421 gtaacagtgc gaataccccc agcaccatta ccaacagcgt gactacgcgg atacggttaa
     6481 tcataaggca ccttcctgaa aacaagttga tctcgttatc ggcaagaagg ggggaaactt
     6541 tattgctgat gccacccgcc gcgaaattga aataaaaaac ccgatgcgca gatcatcggg
     6601 ttcatttcaa ttgaggaaat cgggagaatt acgccacttc tgacgccgcg ctatctaaca
     6661 gcgccatctc ttcgctgttc agcagttttt cgatgttcac cagaatcaac atacggtcgc
     6721 ccagtgcgcc cagtccagtg agatattctg ttgaaagcgt cacggcaaac tctggtgccg
     6781 gacgaatttg ctccgccgtc aatgaaagca cgtctgaaac gccgtcaacc acgatgccga
     6841 ccacccgctg tccgagattc aggacgataa ctaccgtgtt gtcgttatag tccacatcca
     6901 cctggctgaa cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc agattcgtga
     6961 cgcctttgat aaacgctggc gtgttcgcaa tccgtgttac ctgatcgtag ccacggatct
     7021 cctgcacttt caggatatca ataccgtact cttcatcacc aagggtaaat accagaaatt
     7081 cctggcctga cggctcgctg gccagctttg ttacattcgt cataccggtc atattgttac
     7141 ctttttactc attcaggcgg cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa
     7201 ggcggagaca tcaacaatca gtgccacgct gccgtcgcca agaatggtcg cagcagaaat
     7261 gccggggact ttgcgatagt tactttcaag gtttttaacc acaacctggt gttgaccaat
     7321 taattgatcc accagcaagg cgtagcggcg accgccactt tgtaagatca ccacaattcc
     7381 ctgggtggct tcggttttcg cgcccgcgac gttgaacact ttccacagtt cgacgatggg
     7441 cagatattca ccccgcactt ccagcacccg ctcgccgccg gccagtggat ggagatcggc
     7501 ttcacggggt tgcagtgatt ccataacagc attcagcggc agaatgaaaa cttcatccgc
     7561 aacgcgtacg gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa tgcggatcgt
     7621 agtgccagta ccctgcttcg actggatttc gacatgaccg cccatctcct ggatattacg
     7681 tttaacgacg tccatgccga cgccgcgccc ggagacgtcg gtgacctgct ctgccgtgga
     7741 gaagcctggt gcaaatatca gcattgcaac ttcgtcgtcg ctcatgtttt cgctgacagt
     7801 caaaccttgc gaggccgctt ttgccagaat tcgctcacgg tttagccccg ccccatcgtc
     7861 ggtcacttca atgcaaatgt tgccgccctg atgttcggca gacagaatta aatttccgac
     7921 gctgttttta cctgcggcga gccgtttttc tggcagttca ataccgtgat cgaggctatt
     7981 gcgtaccagg tgggtcagcg ggtcgataat gcgttctatc aggctcttgt cgagctcggt
     8041 ggaactgccc accagcgtca gttctacctg cttgccgagt tttcccgcca gatcacgcac
     8101 cagccgggga tagcgactaa agacatattc catcggcatc atgcgaatcg acatcactga
     8161 ttcctgcaaa tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca aatcaccatg
     8221 attaaccggg tccagttcgc tggaacgctg ggcaagcatg gactgggtga taaccagctc
     8281 gccgacgagg ttaattaatt gatcaacctt ttctaccgct acacggatgc tggtggattc
     8341 actgctgcgc gtcgtttttt cccgctccac gcggccggtt ggcgcttgtt cggctgccag
     8401 tttaagcaca ggtggggtag ctatttttgg cgaaacttct actgtttcaa aggtaatctg
     8461 atcggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga tatcgcccgg
     8521 taatattgcc gagagagaat ccgccccttt caccacgtca tttaacgttg tcagatgccc
     8581 cagctcttct tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga taattcgtcg
     8641 cggcaactga ctgcgacttt gctcatcttg cggttcactt ttggcaacca cacttaatcg
     8701 ggtcactgcg gatggcgttt cgcctttcgc ttctaatgcc agttgacgca aggcctggca
     8761 gatataatcg tagctggcgg catccggctc ttgcgactgt ttataagcgt cgagctgttc
     8821 ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga gttgcatctc
     8881 acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct gcaaaacgct
     8941 gaagccaaaa gttcctgccc ctcctttgat cgagtgggca gcccgaaaga tggcattcaa
     9001 ttgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca tatcagccaa
     9061 cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcga tgctcacgct
     9121 gtcacctcgg ttcggctgat ggcattgtgg gaacactgac ctgtggtgca acctcaggtt
     9181 tttctagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt
     9241 cggcttgttt gttcagtacc aacaggctga tgcgacggtt gacggcatca tcaggtccgc
     9301 gatcgcttaa gcgcatcgtt gccgccatgc cgacgacacg taacactttg ccgctatcca
     9361 accctccgac catcagttcg cggcgggatg cattggcccg atcggcagaa agctcccagt
     9421 tgctgtaacc tttctcaccg ctggcgtagg gaaaatcatc ggtatgacct gaaaggctaa
     9481 tacggttggg aataccgttc agcacaggcg cgatggcgcg cagaatgtcg cgcatatagg
     9541 gttcgacatc ggcactgccg gttctaaaca tcgggcgatt ctggctatcg atgatctgaa
     9601 tacgtagacc ttcctggacc agatcgattt tgagatggag acgtaacgcc cgcagtttcg
     9661 gatcggactc tatcaactgg tcgagatcac cccgcaattt ccgcaatcga ctttgctcca
     9721 tgcgtttttt cagctcttcg atgttcggct gcttattcac ttccccctgg ctttgggtgt
     9781 aatcatcacc accgccggga attgggcttt cactattgga aatgcgatcg ccgcccgtaa
     9841 ccgctgtcgc cagtggagtc cggaagtact ccgcaatctg aatcaactct tttgggctgg
     9901 agatggagat cagccacatc accagaaaaa aggccatcat cgcagtcata aagtcggcat
     9961 aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt cgtttgacga
    10021 caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt
    10081 tttcaccgca cgcacatgct cttccagttc aataaacgac ggacgttcgc tggaatagag
    10141 cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat tagaaagcag
    10201 agtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac gtaaaacact
    10261 cgctagtggg gaaataaatc cgtaagccaa taaaataccg aggaaagtcc ccaccatcgc
    10321 atgtgcgata agcgccccca gctcggcggc aggacgatcg gctgaaccta acgcgtgaac
    10381 gccccccatt acagccgcga caataccaaa cgccggaagt gagtccccga ccagcgccag
    10441 actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat ccatcagggc
    10501 ttcgatttcg aaggtgttca tgtgaccgct gataatcagg cgcagataat cgacgataaa
    10561 atcaagcatg acgctatccg cgaggatgcg tgggtagctg gcgaagatct cgctctcacg
    10621 gggattttca atatcacgtt ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa
    10681 ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg agcgacgaaa
    10741 cagcaacggc agcgctttca gcgtgccttt aatcgctttg ccattattgc cgacgataaa
    10801 cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga gtgctccaag
    10861 gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca ggtaacctaa
    10921 taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc agtcgtcagg
    10981 cgttaacgcg cgattgggga aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt
    11041 tgcgccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca gttgtgggat
    11101 aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg gcggttgaca
    11161 taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat tgccgccgca
    11221 gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca atgtccaggc
    11281 acgggttaat gccagcagtg gtccttcttc tgcttgtggg cactgttcaa ggtataaacg
    11341 gtaggctttg atcaccgcat cgacgccatt acacaaaccg gttttcagta aaaactgcca
    11401 tgcattacag aacatcgaag catgaacgtt ttgttcccag gtcataaacc agtcggttga
    11461 gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataaa gttttatcag
    11521 gcgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc ccagggtgat
    11581 caattccatt gccagctgaa tatcccgcgc ttcctgaaca atgctttttt cactcatgat
    11641 caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag caagcgtgtt
    11701 gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc ttgcgtcaac
    11761 tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagctgatt ggtttctgcc
    11821 agcttaacca tttgcggaag
//
LOCUS       DAJTOW010000112        11714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTOW010000112 DAJTOW010000000
VERSION     DAJTOW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.112"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(127..276)
                     /locus_tag="OO244_003297"
     CDS             complement(127..276)
                     /locus_tag="OO244_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665013.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO244_003298"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO244_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5665014.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO244_003299"
     CDS             2367..2669
                     /locus_tag="OO244_003299"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665015.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2735..5701)
                     /locus_tag="OO244_003300"
     CDS             complement(2735..5701)
                     /locus_tag="OO244_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HCR5665016.1"
                     /translation="MLRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP
                     LIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERATLGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSR
                     RLEDGKFRPLRMPGKP"
     gene            complement(5705..6265)
                     /locus_tag="OO244_003301"
     CDS             complement(5705..6265)
                     /locus_tag="OO244_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5665017.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            6575..8152
                     /locus_tag="OO244_003302"
     CDS             6575..8152
                     /locus_tag="OO244_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HCR5665018.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            complement(8490..9983)
                     /locus_tag="OO244_003303"
     CDS             complement(8490..9983)
                     /locus_tag="OO244_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5665019.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(10027..10416)
                     /locus_tag="OO244_003304"
     CDS             complement(10027..10416)
                     /locus_tag="OO244_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665020.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            10634..11074
                     /locus_tag="OO244_003305"
     CDS             10634..11074
                     /locus_tag="OO244_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5665021.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         2591 a         3276 c         3307 g         2540 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggtaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cggcccaaaa tccaccgggg cgtgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catgttcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 cggggagccc gcagaattcg gaaaaaatcg tacgctaagg ttttccgggc atccgtaagg
     2761 gccgaaactt cccgtcttcc agtctgcggc tctgccgcca gacgtaatcg ccggttaggt
     2821 tgatgtgctc ccagcccagc ggcgacagga attgcagcag ctcgccgtcc accggcttgc
     2881 cggcctcgac caaccccagg gtggcgcgtt ccaggtacac cgtgttccac agcacgatag
     2941 ccgccgtcac caggttgagg ccgctggccc ggtagcgctg ctgctcgaag ctccgatccc
     3001 tgatttcccc aaggcggttg aagaacaccg ccctggccag cgagttgcgc gcctcacctt
     3061 tgttcaggcc ggcatgcacg cggcggcgca gttcaacact ttgcagccag tccaggatga
     3121 acagcgtgcg ctcgatccgg cccagctcgc gcagggccac ggccagtccg ttctggcgcg
     3181 ggtagctgcc gagcttgcgc agcatcagcg aggcggtgac ggtgccctgc ttgatcgagc
     3241 tggccaggcg caggatgtcg tcccagtggg cacgcacgtg cttgatgttc agggtgccgc
     3301 cgatcagcgg gcgcaacgtc gggtaggctt gcacgccctg cggcacgtac agcttggttt
     3361 cgccgaggtc gcggatgcgc ggcgcgaagc ggaagcctag caggtgcatc agggcaaaga
     3421 cgtgatcggt gaagccggcc gtgtcggtgt agtgctcctc gatccgcagg tcggactcgt
     3481 ggtacagcag gccgtcgagc acataggtgg aatcgcggac gccgacattc accacgcggg
     3541 tgctgaacgg cgcgtactgg tcggagatat gggtatagaa cagccgtccc ggctcgctac
     3601 cgtacttcgg gttgacgtgc ccggtgctct cgccccggcc acccgcgcgg aagcgctggc
     3661 catcggagga tgaggtcgtg ccgtcgcccc agtgggcggc aaaggcgtgg cgatactggt
     3721 ggttgaccag ctcggccaag gccgccgaat aggtttcgtc gcggatgtgc caggcttgca
     3781 gccaggacag cttggcgtag gtcaggccgg ggctcgactc ggccatcttg gtcagcccga
     3841 ggttgatcgc atcaccgagg attgcggaca gcagcaacgt cctgtctttg gcctcggccc
     3901 cgtccttcaa gtgggtgaag tggcggctga agcccgtcca gtcgtccacg tccatcagca
     3961 gttcggtgat cttgatgcgc ggcagtaact ggctggtttg gtcgatcagc gcctgcgccc
     4021 gatccggcac cgccgcatcc agcggggtga ttttcagccc tgactcggtg aggatggcat
     4081 cgggcagctc gttgtccttg gccaggcggg tgacggtggc caactgctcg tccagcagct
     4141 gcaaacgctc ttccaggtac tggtcgctgt tcgggttgat cgccaggggc agggcctgct
     4201 cgcgcttgag tgcggcgaac ttctcggccg gcagcaggta gtcgtcgaag tcgcggaact
     4261 gccgcgagcc cttgacccag atgtcgccgg agcgcagggc gttcttcagc tcggacaggg
     4321 cgcagatttc gtagaatttc cggtcgaggc cttccggggt gatcaccagc ggcttccagc
     4381 gcggcttgat gaaggccgtg ggtgcatcgg ccggcacctt gcgcaggttg tcggcgttca
     4441 tctcacgcag ggtctgcacg gctgccagca cgccttgcgc ggccggcgcg gcgcgcagtt
     4501 ccagcacctc cagcaaggcc ggcgtgtaac ggcgcagggt ggcgaagttc tcgccgacca
     4561 ggtgcaggtg gtcgaagcct tccggccggg ccagcagctc ggcctcgctg acgctctcgg
     4621 tgaactcgtc ccagggaatc accgcctcga tggcggcata ggggtcgctg ccgctttcct
     4681 tcgcttccag cagcgcctgg ccgatcctgg agtacaggcg caccttgtcg ttgatcgcct
     4741 tgccctgctt ctggaactgc tgctgatgct tgtgcttcgc gccgctgaac agcttgacca
     4801 ggatgcggtc atgcagatcg accagctcat cgatcacggt cgcggtgctc tccagcacca
     4861 cggcggccag ggtcgcgtag cggcgctgcg gctcgaactt accgaggtct ttgggcgtca
     4921 tctgcccacc ctcgcgggcc agcttgagca ggcggttctg gtggatgtgc cggcccaggc
     4981 cttcgggcaa gtccaccaac tgaaatgtct tcagccgctc gatgtgttcc agcatgtgcc
     5041 gagagttggg tttcagcggt gcctggcgca gccaggtcaa ccaggtgatg ctgctgccgg
     5101 ccttgagctt caacagctcg tccagcttgg cccgatgcga gtccgtgagt ggttcgacca
     5161 gggcgcggta gacccgccga ttggctcgcg caatggcttc cgagcaggcc cggtcaatca
     5221 cgctcagcgc cggcaggatg cgtcgtttct gccgtaggct ctccagggcc tgaccggcca
     5281 gcagcaagcc tttgtcggtc tgctgggcca gctcggttag ctcgcgcacc agggcgcgga
     5341 agtcggacag gccgaacggg gccagttgca ggtaggtgcg cagttcctgg gcatgctcgc
     5401 gacgggtcac gtcgcgctcg ccgtactttg cccagctcgc cggctcggcc tggacttgct
     5461 tcgccaccca caggatgacc ggctcgggca gctcgctgtc ggttcccagc gcgtaaccgg
     5521 ggtagcgcag caggcagagc tgcacggcga agccgaggcg gttggcgtcg ccgcgtcgct
     5581 ggcggatcag cgacaggtcg gagtcgttga aggtgtagta gcggatcagg tcatcctggc
     5641 tttccggcag cgcaagcagg gtgtcccgct ccgtggccga gaggatcaag cgacgcagca
     5701 tgtgtcagtc gtccgtgcgg aggtactggt agagggtttc ccggctgatg ttgaactcgc
     5761 gggcaagctg cgccttgggc tcgccggccg tcgctcgctg ccgcagggta gcagcctgct
     5821 catcggacag ggctttcttg cggccccggt acgcgccacg ctgcttggcc aaggcgatgc
     5881 cctcacgctg ccgctcgcgg atcagggcgc gctcgaactc agcgaaggcc cccatcaccg
     5941 acagcatcag gttggccatc ggcgagtcct cgccagtgaa caccaggccc tccttcagga
     6001 actcgatgcg cacgccgcgc tgagtcagct tctgtaccaa gcgacgcagg tcatcgaggt
     6061 tgcgggccag ccgatccatg ctgtgcacca ccactgtatc gccttcgcgg acgaagctca
     6121 gcagcgcttc gagctggggg cgctgggtgt ccttgcccga tgccttgtcg gtgaacacct
     6181 tgctcacctg ggtttgttcc agctggcgtt ccgggttctg gtcaaagctg ctgacccgga
     6241 cgtagccgat gcggtgcccc tgcacgatgt ctccttggtt gaaggcggct taagtgcact
     6301 ttctgttccg ttgtgcctca aagcccattt ctgtcaggct gaaatctata accttcgcgg
     6361 gcatgtgtca aaaaatggga aagcagactc tattctgacg aagcggcgcg gccctgcctg
     6421 acatcaagtt agggtatagc ctagattgac atgcgcgatg caacccttaa cttgcttgca
     6481 cctatcgttt ccatgctagc tttatcgtaa cgctcaagaa attgggctta atgccgaaaa
     6541 caataaaata aaacagccaa cccttgaggt tcttatgcgc cagaatttac cagtgacggg
     6601 tcgaaactta gaactcccaa aagatgccaa tattctttcg actacctccc ctcaaagcca
     6661 tatcacgtac gttaatcctg acttcattaa aatcagtggt ttcactgagg aagaactatt
     6721 aggccagcct cacaacatcg taagacaccc agatatgccg cctgctgcat ttgagcatat
     6781 gtggagtaca ttaaaatctg gccgctcatg gatggggcta gtaaaaaatc gctgtaaaaa
     6841 tggcgaccac tattgggtaa gtgcttatgt aacgccaata gctaagaatg gttcgattgt
     6901 tgaataccag tctgtaagga ccaagcctga acctgagcag gttttggctg cggaaaaatt
     6961 atatgctcaa ttgagaagcg ggaaggccgc gaggccgaaa ttggctgcta gcttttccgt
     7021 gaaaatactc ttgctcatat ggggtagtat tatatcaagc gcaatggctg ccggcatgct
     7081 tactgataca tcaataagca gcttattgtt agccacttta atgtcaggaa gcttaagctc
     7141 tgttagtgtt ttggctattc tctctcctct tggaagactg gttgaaagag ccaggaatat
     7201 ttccaataac ccattaagtc aatccctcta cactgggcgc accgatgagt ttggccaaat
     7261 agagtttgct ttacgaatga tgcaagctga aacaggcgcc atagtaggtc gcataggtga
     7321 tgcatcaaat cggcttagcg aacacacccg aggcctacta aaggatattg agtcaagcaa
     7381 tgtacttaca gttgagcagc aggcagagac agatcaaata gcaacggcag taaaccaaat
     7441 ggtggcaagc attcaagagg ttgcgagcaa tgcacagcat gctgcagatg cggccggaag
     7501 agcagacact gagacggcat ctggccagcg tctggtagcc cacacaagcc agtcaatcac
     7561 tgcccttgaa ggtgaaatta ggcaagccac tcaggttatt catgagcttg aaggtcaaag
     7621 taacgagata tcaaaagttc ttgacgttat acgagggatc gccgagcaaa cgaatttgtt
     7681 ggcactcaac gcagcaattg aggccgcgcg tgctggtgag caggggcgtg gttttgctgt
     7741 tgtcgccgat gaggttcgca gtcttgctgc tcgcacacag caatcgacaa cggatattca
     7801 aagcatgatc agcgctctac aagagcgagc gcaatccgct gttacagtca tggagcaaag
     7861 tagtcggcaa gcgcacacga gtgtagctca cgcagaggaa gcagctacag ctcttgatgg
     7921 aattggccaa cgcgttaacg aaattaccga catgaacgcg caaatagcga ctgcggtcga
     7981 gcagcaggga gcagtaagtg aagacataaa ccgcagtatt atcaatatac gcgatgctgc
     8041 tgataccaat gtacagaccg ggcagaataa tttgcaaagt gcgaaatctg tcgctcagtt
     8101 aactagcgct ctgagcgaac tggcaaaaca gttttgggaa aaacgaggat aacgcttttc
     8161 agtatctcga cagggataac ttactttacc atcggttatc ccttttagcc gcacaaattt
     8221 tagccatggc ttttcaggaa gtcagggcta tcagaatggc cttagaaagc ctagtcaaag
     8281 agctgtcacg agaacaccgt tagcttagcg tacgattttt tccgaattct gcggttcccc
     8341 ccaatccggc tgacggggag ccagcaacgc tgaaaattta ccctcctctt tcccactagc
     8401 ggctcctttt ccgacaacca gcacggcgga tccctgccgc ggcgctgtga acgcagcatt
     8461 ttgattggta tcgttggcct tcaggctcgt cagtcaaaca gacccaggag cagctcagcc
     8521 ggtggcgccc ggctttcggg taacgccctg gtcccgctgg tttcggcttt gtgcttcagg
     8581 tgatcaagga tctgcttgat cactataggg tcttcaatgc aggcgatgac tttcatggcg
     8641 ccgccgcagc cgctgcaggt ctcgatgtcg atattgaaaa cacgcttgag ccgttgcgcc
     8701 catgtcatcg acgctcgccg ttgtgctggt gttgccggtt catcagccac cctgaccttg
     8761 ttgcccctgc cccgttttgc cggcgtgacc aacgcccggt gccgactgtt gggtgcgaac
     8821 accccgtgga agcgggttag gttgactctg ggcttcggta ccagggcggc cagccttgca
     8881 atgaaatcca atggttcgaa aatgacgtgc gtggtgccgt cccggtacgg cgtcttgagc
     8941 tggtagcgca cgttgccgcc tcgtgttaac gacagccgct tctcggatac cgccgggcgg
     9001 ctgatgtacc ggcacagccg ttcgagcttc ttgcgttcat cggccctggc cgccacgccg
     9061 gcgtgcaggc tggacccggc taccttgcca atcccgtcac cgaacggatc accactggtc
     9121 ggcagagttt gcaaagtgaa cacctttcgc cccgcctgtg aaccgacagc gatacggtaa
     9181 gtgatcgagt gccccagcag gggtgtcatc gggtcgtcat ccaccgcatc cgaggccaga
     9241 tagctgtttt cgacatcccg ttccagcagg ccttgccgtt ccagatagcg acccacccgg
     9301 tgggcgatgg tgtgcgtcag ctgggtgagc tctgggctgg tcggcgcctt gacccagcgg
     9361 aaacgcgctg agccgtggga ttgctcgaca tacacaccgt cgagaaacag catgtggaag
     9421 tgaacattca gattgagcgc cgatccaaaa cgctggatca gggtgaccgc gcccgtcttg
     9481 gccacttggt gggtatgacc cgctttcttg accaggtgcg tggcaatgac gcggtaaacg
     9541 atgcccagca cccaccccat gatctcgggc cggctggcaa acaggaaacg cagctgaaac
     9601 gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc aaccagcaag
     9661 gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg acgcttacag
     9721 ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc
     9781 tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc
     9841 tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag
     9901 agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc
     9961 gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc
    10021 tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg
    10081 ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt acatgttatt
    10141 gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga cagaattaat
    10201 attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa aggatgcagt
    10261 ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa aactacctcc
    10321 cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca tgtgaccaaa
    10381 accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt tgacttttaa
    10441 aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa atactacgcc
    10501 cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac gggcagcctc
    10561 agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac acacaggaga
    10621 cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa caggaaagtc
    10681 gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca caggaaaaaa
    10741 tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc tgcgcacttc
    10801 tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc aggcggaaaa
    10861 tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg ttaccctaat
    10921 gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg agagggaaca
    10981 actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa agcggggatt
    11041 ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct gatttaccat
    11101 aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg gaaaccggta
    11161 aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca
    11221 tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac accattcagc cggggtaagt
    11281 cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg
    11341 ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca gcacgttcag gaagggggtg
    11401 atgtacaaat ggcacagagg atgaaagtga cgtatgagct gaaggaatga tgtaaaagtg
    11461 atgtaaaagt gatgtataag tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg
    11521 agcgatgtaa ttctgtagtc aggaagaata acccgttcat gcaggcatga acgatacagt
    11581 gaggaacgct ttttttgtca cgatctcccc ttaacctgcg taaatcaccc gccatggttg
    11641 attatttttc aacctcaaaa tggtcataca cctaacgcag ccgcgatttt catcgcaact
    11701 gcatgaatta aaag
//
LOCUS       DAJTOW010000113        11682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTOW010000113 DAJTOW010000000
VERSION     DAJTOW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.113"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..346
                     /locus_tag="OO244_003306"
     CDS             <1..346
                     /locus_tag="OO244_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5665022.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO244_003307"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO244_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5665023.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO244_003308"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO244_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5665024.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO244_003309"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO244_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5665025.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO244_003310"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO244_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5665026.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO244_003311"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO244_003311"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5665027.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO244_003312"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO244_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5665028.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO244_003313"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO244_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5665029.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO244_003314"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO244_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5665030.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO244_003315"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO244_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5665031.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO244_003316"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO244_003316"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5665032.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO244_003317"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO244_003317"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5665033.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO244_003318"
     CDS             complement(8961..9236)
                     /locus_tag="OO244_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5665034.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO244_003319"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO244_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5665035.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO244_003320"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO244_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5665036.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO244_003321"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO244_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5665037.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO244_003322"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO244_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5665038.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="OO244_003323"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="OO244_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2873 c         3087 g         2955 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc cgtgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatctta ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgcgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAJTOW010000114        11659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTOW010000114 DAJTOW010000000
VERSION     DAJTOW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.114"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            598..795
                     /locus_tag="OO244_003324"
     CDS             598..795
                     /locus_tag="OO244_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665039.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(745..1938)
                     /locus_tag="OO244_003325"
     CDS             complement(745..1938)
                     /locus_tag="OO244_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5665040.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2074..3798
                     /gene="iucA"
                     /locus_tag="OO244_003326"
     CDS             2074..3798
                     /gene="iucA"
                     /locus_tag="OO244_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5665041.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3799..4746
                     /gene="iucB"
                     /locus_tag="OO244_003327"
     CDS             3799..4746
                     /gene="iucB"
                     /locus_tag="OO244_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5665042.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4746..6488
                     /gene="iucC"
                     /locus_tag="OO244_003328"
     CDS             4746..6488
                     /gene="iucC"
                     /locus_tag="OO244_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5665043.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6485..7822
                     /gene="iucD"
                     /locus_tag="OO244_003329"
     CDS             6485..7822
                     /gene="iucD"
                     /locus_tag="OO244_003329"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5665044.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7828..10023
                     /gene="iutA"
                     /locus_tag="OO244_003330"
     CDS             7828..10023
                     /gene="iutA"
                     /locus_tag="OO244_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5665045.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10574..10717)
                     /locus_tag="OO244_003331"
     CDS             complement(10574..10717)
                     /locus_tag="OO244_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665046.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10956..>11659
                     /locus_tag="OO244_003332"
     CDS             10956..>11659
                     /locus_tag="OO244_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5665047.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2955 a         2900 c         2993 g         2811 t
ORIGIN      
        1 tggagtgaaa caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa
       61 atcatgactc ctcacaaaga aaatatcact gaaagggctc agcagtttaa aagagtttta
      121 gttttacagg atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt
      181 cccaaaaaac ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag
      241 tcaggtgttt gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc
      301 agtaaaaata cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa
      361 aaagaaacct ggcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc
      421 cctgataccc atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa
      481 cgcgcagcgc agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac
      541 agagcaacgc tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg
      601 gagattacac cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc
      661 agaacactgc ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac
      721 tccagtaagg tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga
      781 cacaatggta aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc
      841 tgttttcgat accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt
      901 agcgagccag cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc
      961 gggtgttgcc agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag
     1021 ctgttcggag agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag
     1081 ggccaccgga gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac
     1141 tgcggacaga cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtagcgaa
     1201 ctggcgagta agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa
     1261 tattgcgcaa agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg
     1321 tgtgggctgt ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggggc
     1381 tgccatcagc agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag
     1441 cggcccgaat agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg
     1501 attcccttct ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac
     1561 ggtcagcccg taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat
     1621 ggcgctggta gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc
     1681 tcccagagcc cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca
     1741 cggggacgat ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag
     1801 actcagattt gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat
     1861 accgagaagc ccggcactga acgcaagtgg ccagcaggac agtggttttt ggggcgtatc
     1921 ttcgatccct ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa
     1981 aattaattta tctgacaata acatttattg ttgataatga gaatcattat tgacataatt
     2041 gttattattt tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc
     2101 acagatgtgg ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa
     2161 ctgacagaat acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat
     2221 ttcagagtgg cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg
     2281 gtcacggcat caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat
     2341 aagctacgcc atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg
     2401 ctggaaagcc atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg
     2461 cgtgaaaaag cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc
     2521 caccctgcgc ctaagtctca tgaaccgttt aaccggcggg aggctgaacg ctacctgcct
     2581 gatatggcac cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt
     2641 gaaagtttgc atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct
     2701 cagttactca acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg
     2761 gaatatcttt tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac
     2821 ttaggtgagg ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc
     2881 accagccgcg atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact
     2941 ctgtccgtga aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc
     3001 tggcagatgc tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc
     3061 gggctgctcg atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat
     3121 ctgctggtgg atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca
     3181 cctgatggcg gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt
     3241 atcactgtgc aacaggccgc ccatgcatgg gttgatgcat actgtcagca agtgctaaag
     3301 ccgctgttta cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt
     3361 cttgtccaga tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc
     3421 gcttttatgc ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat
     3481 attttcaccc atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact
     3541 tttgctgtga ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct
     3601 cgtgtacgag catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac
     3661 tacgtgctgg aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat
     3721 cataacgaga acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg
     3781 caggctcagg aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc
     3841 tgtgaaaaac tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg
     3901 cactgtcccg gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc
     3961 gccgcaccac aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag
     4021 gcgctgacgc tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag
     4081 ttaccgttat ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca
     4141 gagcgtgaga tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt
     4201 tacgatccac gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca
     4261 gaacgtttca cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc
     4321 tcactggagg tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg
     4381 ctgattgggt gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa
     4441 gaccgcattg gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt
     4501 ggtgaacagc aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat
     4561 tacctgctgc tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac
     4621 cagcgcctgt tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc
     4681 ccacataagc gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt
     4741 ctgtaatgaa tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt
     4801 ctgagatgga gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca
     4861 ttaacctgcc gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt
     4921 ggattgatgc ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga
     4981 tgcagctaaa gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt
     5041 tgtatgccac gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca
     5101 gtgacctgat taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat
     5161 tcgtttttaa taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag
     5221 agtataccaa caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc
     5281 gctgcgataa cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt
     5341 ttacacgctt cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctgccgctgc
     5401 cggtacatcc gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgccg
     5461 aaggcaggat ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc
     5521 gtaccctgac caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct
     5581 acaatacctc atgctaccgg gggatccctg gcagatatat cgctgccgga ccactggctt
     5641 cacgctggct gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga
     5701 tccttggtga accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg
     5761 ctccctatcg ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc
     5821 tgaaaccgga tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc
     5881 agccgctggc aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc
     5941 aattgttccg ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc
     6001 ttattgcaca cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc
     6061 tgctgaaaga cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact
     6121 ctttgcctca ggaggtgcgt gatgtcacat cccgtctgag tgcggactac ttaatccatg
     6181 atttgcagac gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc
     6241 ttggcgtacc cgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga
     6301 acaaacatcc acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca
     6361 ttcgcgtggt gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca
     6421 tgctgccgaa ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg
     6481 aatcatgaaa aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat
     6541 tgctgcattg tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca
     6601 tttttcctgg catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa
     6661 agatctggtc agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa
     6721 gcacaaaaag ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt
     6781 ttctgactat ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt
     6841 tgaaaatatt gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga
     6901 atattttgcc cacaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt
     6961 gaagcatgtg acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct
     7021 gagtggaaaa cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa
     7081 tgcattacgc ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa
     7141 ttttaacgca ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc
     7201 aggcttctcc ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac
     7261 atcggatggc atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt
     7321 tgaagttctg agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaactct
     7381 ggaaagtagt ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga
     7441 gagcctggaa agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat
     7501 acttccctca ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga
     7561 tgacttcact ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag
     7621 catgcaaact catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg
     7681 tattcttaat cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat
     7741 tcagtggcgc agcggcagcc ggaaaaaacc acagccggag gctgcttctt taactcacta
     7801 tacagcaaat attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt
     7861 ccgctgcttc ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc
     7921 atagtgtctg ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt
     7981 atcgaaaatg ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg
     8041 gctcagttaa tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac
     8101 atgcgtggcc gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc
     8161 gacagccgac aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc
     8221 ggcgcgacgg ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa
     8281 aaaggtcagc cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc
     8341 agtaaagatc acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc
     8401 ggacgtcttt ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc
     8461 accctgcttg ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga
     8521 accggtacgc tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa
     8581 agtcagggag acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg
     8641 agcagcacac catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg
     8701 catttgatca gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag
     8761 gtttactacc gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa
     8821 caggcgacgg ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact
     8881 ctgaacagcc aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag
     8941 cgctttacct ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg
     9001 aacaaccata agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg
     9061 gccttcctgc aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc
     9121 tatcagtata ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt
     9181 gctaccggga aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac
     9241 tttctgttca atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat
     9301 ttttcccagg gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt
     9361 gcagcagtga acggccatct tcccctgaca aagagcgtga acgtcagcga cagtaagctg
     9421 gaaggcgtga aagtcgattc ttatgaactg ggctggcgct ttatcggtga caacctgcgg
     9481 actcaaatcg cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg
     9541 accatcagtg tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac
     9601 ctgatcccgg atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg
     9661 aaagtgaacg gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca
     9721 gcttacatta actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc
     9781 gacgtaagcg atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt
     9841 agttggcagc ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac
     9901 tataccactg tctggggaca gcgtgcacct ctgtactaca gcccgggtta cggccctgct
     9961 tcactgtacg actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc
    10021 tgacggggat gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg
    10081 ttgtttacag ccagtataat atacagcctt aacaagatat tccgtctccg gaatgggctg
    10141 ttcaccactg aataacatta tgtctgtttt atggatcttt gactgaaatt tatcgcatat
    10201 cctcatttca ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc
    10261 tggcagatcc tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt
    10321 gctgcaatga aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta
    10381 aaaagaggta cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc
    10441 tgtcataaag tccggtaatg gagattgtta atacgatggt gcacgatgca tcgtcagtgc
    10501 ccggcccgga ggatgccccg tagctacata cggatcaggg ttggcaatat cgaatggcag
    10561 gctataggca aagttaaagc cccatggagt agcacaaaat atgccgcaca aaggaaacgg
    10621 tctgaataac gcagtgatgg agaacttctt cggcacactg caaaatcgca tcgtggtcga
    10681 tcgctaattc gtcgtgaatc actgaacgat gttccatgcg aacagatatt cgatcatatc
    10741 gaaatattaa acattacccc tccccacatt gggcaaaaaa aaacaaaaaa cgatcaatta
    10801 gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt tacaatatag
    10861 aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt aattcttaat
    10921 actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac tcccttaaat
    10981 atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga gtttctggta
    11041 aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt acagtaaatg
    11101 cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt gcacaaaata
    11161 aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac ggagataaat
    11221 tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt ggcgcagcta
    11281 ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac cctcaggccg
    11341 cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga aattccaatg
    11401 attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc accccggcag
    11461 agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc acttctgacg
    11521 gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt attaatggtg
    11581 aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct agtctatttg
    11641 aggtcaccca atgggactc
//
LOCUS       DAJTOW010000115        11618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTOW010000115 DAJTOW010000000
VERSION     DAJTOW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.115"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..598
                     /locus_tag="OO244_003333"
     CDS             <1..598
                     /locus_tag="OO244_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5665048.1"
                     /translation="EMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSG
                     QKKLVQLFDSGEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPY
                     LAEEGTETVGVTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVT
                     LSGLVATVKGVKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            657..1043
                     /locus_tag="OO244_003334"
     CDS             657..1043
                     /locus_tag="OO244_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5665049.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1052..1381
                     /locus_tag="OO244_003335"
     CDS             1052..1381
                     /locus_tag="OO244_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5665050.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1353..4394
                     /locus_tag="OO244_003336"
     CDS             1353..4394
                     /locus_tag="OO244_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5665051.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4394..4723
                     /locus_tag="OO244_003337"
     CDS             4394..4723
                     /locus_tag="OO244_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665052.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4723..5421
                     /locus_tag="OO244_003338"
     CDS             4723..5421
                     /locus_tag="OO244_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5665053.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5426..6169
                     /locus_tag="OO244_003339"
     CDS             5426..6169
                     /locus_tag="OO244_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665054.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6106..6708
                     /locus_tag="OO244_003340"
     CDS             6106..6708
                     /locus_tag="OO244_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5665055.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6769..10248
                     /locus_tag="OO244_003341"
     CDS             6769..10248
                     /locus_tag="OO244_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5665056.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSISENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10316..10915
                     /locus_tag="OO244_003342"
     CDS             10316..10915
                     /locus_tag="OO244_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5665057.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            10979..>11618
                     /locus_tag="OO244_003343"
     CDS             10979..>11618
                     /locus_tag="OO244_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5665058.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT         2732 a         2751 c         3737 g         2398 t
ORIGIN      
        1 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
       61 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      121 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      181 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      241 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      301 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      361 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      421 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      481 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      541 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      601 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      661 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      721 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      781 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
      841 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
      901 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
      961 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1021 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1081 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1141 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1201 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1261 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1321 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1381 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1441 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1501 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1561 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1621 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1681 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1741 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1801 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     1861 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     1921 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     1981 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2041 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2101 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2161 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2221 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2281 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2341 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2401 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2461 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2521 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2581 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2641 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2701 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2761 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     2821 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     2881 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     2941 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3001 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3061 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3121 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3181 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3241 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3301 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3361 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3421 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3481 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3541 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3601 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3661 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3721 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3781 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     3841 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     3901 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     3961 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4021 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4081 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4141 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4201 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4261 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4321 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4381 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4441 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4501 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4561 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4621 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4681 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4741 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4801 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     4861 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     4921 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     4981 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5041 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5101 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5161 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5221 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5281 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5341 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5401 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5461 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5521 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5581 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5641 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5701 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5761 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     5821 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     5881 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     5941 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6001 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6061 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6121 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6181 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6241 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6301 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6361 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6421 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6481 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6541 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6601 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6661 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6721 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6781 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     6841 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     6901 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     6961 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7021 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7081 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7141 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7201 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7261 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7321 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7381 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7441 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7501 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7561 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7621 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7681 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7741 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7801 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     7861 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     7921 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     7981 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8041 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8101 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8161 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8221 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8281 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8341 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8401 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8461 acgctgcgcc agcgcctgtt ccgctgcgtg agtatcagtg agaacgacga cggcacgtat
     8521 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8581 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8641 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8701 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8761 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     8821 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     8881 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     8941 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9001 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9061 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9121 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9181 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9241 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9301 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9361 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9421 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9481 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9541 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9601 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9661 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9721 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9781 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
     9841 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
     9901 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
     9961 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10021 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10081 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10141 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10201 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10261 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10321 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10381 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10441 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10501 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10561 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10621 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10681 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10741 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10801 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    10861 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    10921 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    10981 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11041 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11101 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11161 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11221 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11281 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11341 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11401 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11461 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11521 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11581 gaccggcact ctgaaaatgg ctaaccatct gtcagaaa
//
LOCUS       DAJTOW010000116        11526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTOW010000116 DAJTOW010000000
VERSION     DAJTOW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.116"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(217..292)
                     /locus_tag="OO244_003344"
     tRNA            complement(217..292)
                     /locus_tag="OO244_003344"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            852..3104
                     /locus_tag="OO244_003345"
     CDS             852..3104
                     /locus_tag="OO244_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665059.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3440..4372
                     /gene="csiD"
                     /locus_tag="OO244_003346"
     CDS             3440..4372
                     /gene="csiD"
                     /locus_tag="OO244_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5665060.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLSRELMRQRGYFAYATHHYQTHQ"
     gene            4392..5660
                     /gene="lhgO"
                     /locus_tag="OO244_003347"
     CDS             4392..5660
                     /gene="lhgO"
                     /locus_tag="OO244_003347"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5665061.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVQQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5683..7131
                     /gene="gabD"
                     /locus_tag="OO244_003348"
                     /pseudo
     CDS             5683..7131
                     /gene="gabD"
                     /locus_tag="OO244_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7139..8425
                     /gene="gabT"
                     /locus_tag="OO244_003349"
                     /pseudo
     CDS             7139..8425
                     /gene="gabT"
                     /locus_tag="OO244_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8663..10063
                     /gene="gabP"
                     /locus_tag="OO244_003350"
     CDS             8663..10063
                     /gene="gabP"
                     /locus_tag="OO244_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5665062.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10084..10746
                     /gene="csiR"
                     /locus_tag="OO244_003351"
     CDS             10084..10746
                     /gene="csiR"
                     /locus_tag="OO244_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5665063.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10747..11196)
                     /gene="kbp"
                     /locus_tag="OO244_003352"
     CDS             complement(10747..11196)
                     /gene="kbp"
                     /locus_tag="OO244_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5665064.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11280..11438)
                     /locus_tag="OO244_003353"
     CDS             complement(11280..11438)
                     /locus_tag="OO244_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5665065.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2965 a         2829 c         2874 g         2858 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gtaacgttat tatgttttct ataaaaccag gacctggaaa tttacctatc gacaacccca
      901 cattgttatc atggaacatt actgatgggg atctaaattc caaatttaat acattagaat
      961 atctaaactg tataacaaat attattaatg cttgtggagt ttatcctcag gatttaaaag
     1021 acagagaaat tatatcaact tttcacgcag aaaaagtcat taatgatctg ttaaaaaacg
     1081 attataaaat ttccctttct ccagatacaa cttaccgaga gctgaataaa gctgcacaga
     1141 gtagcattac agcgccagac aggataggag aagggaaaac atgggtttat caacgagata
     1201 caatggttga aagaggtgat aacagtggtg ttcatcagta tggtccagct gaacatttca
     1261 ctcacattat atctgacaaa ccgtccccaa aagataagta tgttgcatat gctattaaca
     1321 ttcctgacta tgagctagca gccgatgtat ataatattaa cgtgacgtca ccttccggac
     1381 agcaagaaac atttaaaata ctaatcaatc cagaacatct acggcaaaca cttgagcgta
     1441 aatctcttac tgctgttcag aaatcacaat gtgaaatcat cacccccaaa aaacctggcg
     1501 aagcgattct tcatgctttt aatgccacct accagcaaat cagggaaaat atgtctgagt
     1561 ttgcacgttc ccattatggg tatatacaaa tccctccagt gacaactttc cgcgccgacg
     1621 gaccagaaac tcccgaagaa gaaaaaggtt actggtttca cgcttatcaa cccgaagatc
     1681 tttgtaccat ccataatcca atgggggatt tgcaggattt tatcgcattg gttaaagatg
     1741 ctaaaaaatt tggtatcgat atcattcctg attatacctt taactttatg ggaatcgggg
     1801 gaagtggtaa aaatgacctg gattatccct ctgctgatat acgagcgaag atcagtaaag
     1861 atatagaaag tggtatccct ggctattggc aaggtcaggt tttgattcca ttcattaaag
     1921 atccagtaac aaaagaacgt aaacaaatcc atccagaaga tatacatctc actgcaaaag
     1981 acttcgaagc aagtaaagat aacatctcta aggatgaatg gaaaaacctc catgcattaa
     2041 aagaaaagag tttaaatgga atgcctaaaa caacccccaa aagtgaccag gttattatgt
     2101 tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt acgaggttta cgttatgatg
     2161 cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat aaccccaccg cttaaaaatt
     2221 ataatgagcg attacacaat actaacttat ttaacccaaa atatcatgaa aaaaccgtta
     2281 tgaattacat ggaatatctg gtaacttgtc agttgaatga agaacaaatg tcatcgctgc
     2341 tttatgaaag agatgattta agcgccattg atttttcatt gctcatgaag acgataaaag
     2401 ccttttcatt cgggggagat ctcctaaccc ttgcatcaaa accgggttcc acaatctcaa
     2461 gcatcccatc agaaagacgg atattgatta acattaacca cgattttcct aacaatggca
     2521 atcttttcaa tgactttcta tttaaccatc aacaagatga acaattagca atggcatata
     2581 tggccgctct cccgttcagc aggcctttag tttactggga tggccaggta ttaaaatcaa
     2641 cgactgaaat taaaaattat gatgggtcca cgcgtgtcgg tggtgaggcg tggcttaata
     2701 aaggttgctc tacctatcag cagctctaca atgaattcca cgcattatat atagataaag
     2761 caggaatatg gagcgcattt gagggtgtat ttgcaactaa aaacgttctg gcctttagtc
     2821 gtggggattc tgtgaacatt aatcactctc ctcatgatgg actagttata ataaataaag
     2881 gaaacgaaga agttgaaggt acctggccta acaaattgca acctggaata tataaaaaca
     2941 tggggagtaa tagcgttaac attattatta ataatacccg aaaaattatc cccccttgta
     3001 aagtatttac gcttagaggc ggatctctaa atatcaatat tcctaggcgt agcgctcttc
     3061 ttttagggaa aacaggagaa ccgccgaact atctctattt ataatttaat gttatatctg
     3121 ccccgataaa acggggcaga taatatgttt agtttactaa cggtcatttt gtagtgaagc
     3181 catttactgt tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg
     3241 ttgctttagt tattcatttc ctgtctcact ttgccttaat accctacgtt aaatgttact
     3301 aatttgttgc ttttgatcac aataagaaag caatatgtcg cttttgtgcg catttttcag
     3361 aaatgtagat atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct
     3421 cataagagga tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc
     3481 aggccaggac tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga
     3541 actcaccttc accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt
     3601 gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct
     3661 gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc
     3721 gctgttgatc aatgcggtag gtatcgatga tgtggcgcag gcagatgaga tggtgaagct
     3781 ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta
     3841 ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca
     3901 ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct
     3961 gatgatgaaa atcgatgact gggaacatct ggaccactat ttccgccatc cactggcgcg
     4021 tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc agcaaagatg ttttccatcc
     4081 agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc tatatcgacc agttcgtcca
     4141 gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt tccgatgcca ttgaaaccag
     4201 caaaggcatt ctttctgtac ccgttcccgt tggcaaattc ctgttgataa acaacctgtt
     4261 ctggctgcac ggtcgcgacc gctttactcc gcacccggat ctgagccgtg aactgatgcg
     4321 tcagcgtggc tatttcgctt acgccactca ccactaccag acgcatcagt aagcgcaaag
     4381 gaattgagcg gatgtatgat tttgtgatta ttggcggcgg catcatcggc atgtcgaccg
     4441 ccatgcaact gattgatgtt tatccggatg cccgcattgc gttgctggaa aaagagtccg
     4501 gcccggcctg tcaccagacg ggccacaaca gcggcgtgat ccatgccggg gtctattaca
     4561 cgcccggcag cctgaaggcg cagttttgcc tggcggggaa ccgtgccacc aaagcctttt
     4621 gcgatcaaaa cggcattcgc tatgacaact gcggcaagat gctggtcgcc acgtctcagc
     4681 tcgaaatgga acggatgcgc gccttatggg aacgcacagc ggcgaacggt atcgagcgcg
     4741 agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa tatcaccggg cttggtggca
     4801 tttttgtgcc gtccagcggc attgtcagct accgcgaagt gacggcggca atggcaaaaa
     4861 tcttccaggc cagaggcggc gagattattt ataacgccga agtcagcgcc ctcagtgagc
     4921 ataaaaacgg cgtggtgata cgcacccgtc agggcggtga atatgaagca tcgacgctga
     4981 ttagctgttc cgggctgatg gctgaccggc tggtgaaaat gctcggcctc gaaccgggct
     5041 ttattatctg cccgttccgt ggcgagtatt tccgacttgc gccggagcat aaccagattg
     5101 ttaaccacct gatttacccc attcccgacc ccgcgatgcc atttttgggc gtgcatctca
     5161 cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc ggtgctggct ttcaaacgcg
     5221 aaggctatcg caagcgcgac ttctcattta gtgacacgct ggagattttg agctcgtcgg
     5281 ggattcgccg ggtgcagcaa aaccatctac gctcaggact gggcgagatg aaaaactcgc
     5341 tgtgcaaaag cggctatctg cggctggtgc aaaagtattg tccccggctt tcgttaagcg
     5401 atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt atcgccggac ggcaagctga
     5461 ttgacgattt tctgtttgtc accaccccac gcacgatcca cacctgcaat gcgccctccc
     5521 cggcagcgac atcagcaatt cctattggtg cgcatattgt cagcaaggta caaacgctgt
     5581 tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc acgtagtgtg gatgccttac
     5641 acgccgcatt taatcaataa cctttgaaaa caggacgtaa caatgaaact taacgacagt
     5701 aacttattcc gccagcaggc gttgattaac ggggaatggc tggacgccaa caatggtgaa
     5761 gtcatcgacg tcaccaatcc ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc
     5821 gcagatgaaa cccgcgccgc tatcgacgcc gccaaccgcg ccctgcccgc ctggcgtgcg
     5881 ctcaccgcca aagaacgcgc caccattctg cgcaactggt tcaatttgat gatgcagcat
     5941 caggacgatt tagcgcgcct gatgaccctc gaacagggta aaccgctggc tgaagcgaaa
     6001 ggcgaaatca gctacgccgc ctccttcatt gagtggtttg ctgaagaagg caaacgtatt
     6061 tatggcgaca ccattcctgg tcatcaggcc gataagcgcc tgattgttat caagcagccg
     6121 attggcgtca ccgccgccat cacgccgtgg aactttccgg cggcgatgat tacccgtaaa
     6181 gccggtccgg cgctggcggc aggctgcacc atggtgctga agcccgccag tcagacgccg
     6241 ttctctgcgc tggcgctggc ggaactggcg attcgcgcgg gcattccggc tggcgtgttt
     6301 aacgtggtca ccggttcggc aggcgcggtc ggtaatgaac tgaccagcaa cccgctggta
     6361 cgcaaactgt cgtttaccgg ttagaccgag attggccgcc agttaatgga acagtgcgcg
     6421 aaagacatca aaaaagtgtc gctggagctg ggcggcaacg cgccgtttat cgtctttgac
     6481 gatgccgatc tcgataaagc cgtggaaggc gcgctggcct cgaaattccg caacgccggg
     6541 caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc
     6601 gaaaaattac agcaggcggg gagcaaactg cacatcggcg acgggctgga gaaaggcgtc
     6661 accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag tggaagagca tattgccgat
     6721 gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag cgcacgaacg cggcggcaac
     6781 ttcttccagc caaccattct ggtggacgtt ccggccaacg ccaaagtgtc gaaagaagag
     6841 acgttcggcc ccctcgcccc actgttccgt tttaaagatg aagccgatgt gattgcacaa
     6901 gccaatgaca ccgagtttgg tcttgccgcc tatttctacg cccgtgattt aagccgcgtc
     6961 ttccgcgtcg gcgaggcgct ggagtacggc atcgtcggca tcaataccgg cattatttcc
     7021 aatgaagtgg ccccattcgg cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag
     7081 tatggcatcg aagattactt agaaatcaaa tatatgtgca tcggtcttta actggagaat
     7141 gcgaaggaac agcaataaag agttaatgca gcgccgcagt caggcgattc cccgtggcgt
     7201 tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac tgccgggtgt gggacgttta
     7261 aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg ctcaataccg ggcacctgca
     7321 tccgaaggtg gtggccgcgg tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca
     7381 ggtgctggct tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg
     7441 cgatttcgcc aagaaaacgc tgctggttac gaccggttcc gaagcggtgg aaaacgcggt
     7501 aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg gcgagtatca
     7561 cgggcgcacg cattacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat
     7621 ggggctgatg ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc acggcataag
     7681 cgaggatgac gctatcgcca gcatccaccg gatcttcaaa aatgatgccg cgccggaaga
     7741 tatcgccgcc atcatgattg agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc
     7801 agcctttatg cagcgtttac gcgctctgtg tgacgagcac gggatcatgc tgattgccga
     7861 tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt
     7921 ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt
     7981 caccgggcgc gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc
     8041 cggtaacccg attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa
     8101 tctgctgcaa aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc
     8161 cgaaaaacac ccggagatcg gcgacgtacg cgggctggga tcgatgattg ccatcgagct
     8221 gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg
     8281 cgcccgcgat aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat
     8341 ccttgtacca ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca
     8401 gtgttttgat gaggcgaagc agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct
     8461 tgtccggcct acgagaatcc aagtcggata aggcgtttac gccgcatccg acaatctgta
     8521 cgtgaacagg aataaatcta tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga
     8581 atcaataaca attaaacgcg tgacccgacg cgggaaatgt cggggcgctc tcccaagtga
     8641 cacactttcg agaggattca ggatggggca atcatcgcaa ccacacgagt taggcggcgg
     8701 gctgaaatca cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt
     8761 tgtcggttcc agcgtcgcca tcgccgaagc ggacccggcg gtattactgg cctatctgtt
     8821 cgccggatta ctggtggtta tgattatgcg gatgctggcg gaaatggcag ttgccacgcc
     8881 cgataccggt tcgttttcca cctatgccga taaagccatt ggccgctggg cgggctatac
     8941 catcggctgg ttgtactggt ggttttgggt actggttatc ccactggaag ccaacatcgc
     9001 cgccatgatc ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat
     9061 taccctcgcc ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt
     9121 ctggctggcg ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc
     9181 aattagcggt ttttaccctt atgccgaagt gagcgggatc tcaagattgt gggatagcgg
     9241 cggctttatg cccaacggtt tcggtgcggt attaagcgcg atgttgatca ccatgttctc
     9301 gtttatgggc gcagaaattg tcaccattgc cgccgcggaa tccgacatgc cggaaaaaca
     9361 tattgtccgc gccaccaact cggttatctg gcgtatttct atcttctatt tgtgctctat
     9421 ttttgtcgtc gtggcattaa taccgtggaa tatgccgggg ctgaaagccg ttggatctta
     9481 tcgctcggtt ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat
     9541 attactttcc gtaaccagtt gcctgaactc ggccctgtat accgcctcaa ggatgctcta
     9601 ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac
     9661 cccgtacgtg gcggtgttac tctccaccgg cgcagcattc ttaacggtgg tggtgaacta
     9721 ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct
     9781 ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg
     9841 aagcgaaatt cgcttgcgaa tgtggcttga tccctggctc acctggctgg tcatcggctt
     9901 tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag aagtgatctc
     9961 caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc gctggaaaaa
    10021 gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat caggagtcac
    10081 accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat tattcgcggt
    10141 aattttcaac cggatgaaaa attacggatg agtttactga catcgcgtta tgcacttggc
    10201 gttgggccgt tgcaggaagc actttctcaa ctggtggcgg aacggctggt cacggtggtg
    10261 aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga tattttcgac
    10321 gcccgcgcca atatggaagc gatgctggtc agtctggcga ttgcccgcgg tggcgatgaa
    10381 tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc ctgtgacgcc
    10441 agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac agcaattgtg
    10501 gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg
    10561 cgttatcgat ttatctggct gcggcgaacg gtgctttcgg tggaaatgct ggaggataaa
    10621 cacgatcagc accagaccct gactgcggcg gtactgccgc gagatacagc gcgcgccagt
    10681 gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc gatggctggc
    10741 aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat ccgggctttt
    10801 tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac cgtagacctg
    10861 tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc tggcagtggc
    10921 tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat tccccaccgc
    10981 aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg tcgctttgcc
    11041 gtcggcaatt tgaatgttca ctttatcggc atcaggtata ccggttttgc tcagatgctc
    11101 ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcat cccagagttt
    11161 ttctccggca tctttaacaa aattgaacag acccatagct acctcattcg ttaacggaaa
    11221 cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt
    11281 taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc
    11341 agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac gccgatcggc
    11401 ggcagaatga tggtgatgac gattctccag aaacccatat gtactcccta taagaaaatt
    11461 actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg gagcctgcca
    11521 gtttca
//
LOCUS       DAJTOW010000117        11376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTOW010000117 DAJTOW010000000
VERSION     DAJTOW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.117"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            319..1959
                     /gene="trg"
                     /locus_tag="OO244_003354"
     CDS             319..1959
                     /gene="trg"
                     /locus_tag="OO244_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5665066.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1997..2920)
                     /locus_tag="OO244_003355"
     CDS             complement(1997..2920)
                     /locus_tag="OO244_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5665067.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3137..4480
                     /locus_tag="OO244_003356"
     CDS             3137..4480
                     /locus_tag="OO244_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5665068.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4705..6360
                     /gene="opgD"
                     /locus_tag="OO244_003357"
                     /gene_synonym="mdoD"
     CDS             4705..6360
                     /gene="opgD"
                     /locus_tag="OO244_003357"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5665069.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6500..6724
                     /locus_tag="OO244_003358"
     CDS             6500..6724
                     /locus_tag="OO244_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5665070.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6787..7323
                     /gene="rimL"
                     /locus_tag="OO244_003359"
     CDS             6787..7323
                     /gene="rimL"
                     /locus_tag="OO244_003359"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5665071.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7318..8298)
                     /locus_tag="OO244_003360"
     CDS             complement(7318..8298)
                     /locus_tag="OO244_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665072.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8422..9414
                     /gene="tehA"
                     /locus_tag="OO244_003361"
     CDS             8422..9414
                     /gene="tehA"
                     /locus_tag="OO244_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5665073.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9411..10004
                     /gene="tehB"
                     /locus_tag="OO244_003362"
     CDS             9411..10004
                     /gene="tehB"
                     /locus_tag="OO244_003362"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5665074.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10307..10963
                     /locus_tag="OO244_003363"
     CDS             10307..10963
                     /locus_tag="OO244_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5665075.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10981..11376)
                     /gene="tnpA"
                     /locus_tag="OO244_003364"
                     /pseudo
     CDS             complement(10981..11376)
                     /gene="tnpA"
                     /locus_tag="OO244_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2933 a         2661 c         2858 g         2924 t
ORIGIN      
        1 atatcggtcg gggccttcgt ctttctgatt cccggttagc ctgaaaacag aaagtctcag
       61 gcacccgcag gcatcctatg aggtttcctt agggacgaaa ataatcactt cacgaaattg
      121 cgtgctgttt tccagaattt ttcgtcattc gggttagcca gtttagccat tcgttactct
      181 cttcattcca atagcattaa ttttctctgc aataattgtt gtaaaaatgt gacgcaaaga
      241 ggtttttggt cataagtaat taccgtcaag tgccgatgac tttctatcag gagtaaacct
      301 ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt tttgcatcac
      361 atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct attcgcatta
      421 agttctgctc tggctggcta tttcctctgg caggccgatc gcgatcagcg tgatgttact
      481 gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg
      541 atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcga
      601 aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg tgcttatcag
      661 aatcgaccgg tgaaaacacc tgctgatgaa gccttcgaca ctgaattaaa tcaacgcttt
      721 caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa
      781 gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt
      841 ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga actggcccat
      901 cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcttgc cctggtcatg
      961 acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca
     1021 caacggattg aaaaaatcgc cagtggcgat ctgacgatga atgatgaacc ggcgggtcgt
     1081 aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact ggggatgaca
     1141 gtagggactg ttcgacaggg cgcggaagag atttatcgtg gcaccagcga aatttcagct
     1201 ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga acaaactgcc
     1261 gccagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc
     1321 agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggtttccggt
     1381 gtagtaaaaa cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc
     1441 gtcatcaaca gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc
     1501 gcgcgagcgg gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt acggacactc
     1561 gcaagccgca gcgctcaggc agcgaaagag attgaaggct tgatcagtga atcagtcagg
     1621 ttaattgacc tggggtcgga tgaggtggca acggcaggga aaaccatgag cactattgtt
     1681 gatgccgtcg cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa
     1741 agtagaggca taactcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag
     1801 aatgcttctc tggtagaaga ggcctcagca gcggcggtgt cccttgaaga acaggctgca
     1861 cgattaactg aggcggtgaa cgtattccgt ctgaacaaac attctgtgtc ggcagaacct
     1921 cgcggagcgg gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga
     1981 cagatccctt cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc
     2041 aaccagaaga gaacgtcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat
     2101 gtcccggaac aggcagggcc accagcgtgg catgacgcag gtcgtctttt acagcgccag
     2161 aagggacaaa ccacacgtaa tcgtattcaa cggtaagttg acgagatagc gaagtagaca
     2221 gcgtttcgat acaacccgaa ggaattttac agccctggct ctgcactaat gcatctgaat
     2281 gctggcgtgg cgcagtgcct tctggtgata caacgaccgg ccattccagc acccggctta
     2341 gcgttacgtt ctcctgaagt agcgggtgat tagggcggac aaccagcttc aacgattcaa
     2401 gaaacagcag ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc
     2461 cgatatcgat ttccccggtt tttaaacccg ccagaatcat agggttactc attgtcgcaa
     2521 cttgcaaggt cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc
     2581 ccagtgccgc agtaggtagt gcaccaaccc tgacgacatc attattaaga ccttctttac
     2641 gatgaagcga ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa
     2701 attgttcgcc gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc
     2761 ccgtcagctg ctccagttca ttcaatgtat tagagagcgc aggttgactc aaattaaggg
     2821 tttcagctgc gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc
     2881 gcaaacggat gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag
     2941 cggtgtcgct gacaagtgaa gttgtttgat tatgataact tgattgcaaa atattattaa
     3001 caattaaagc aattatgtta cagcaaaatg gataatattg atgttttcgc ggcgagatca
     3061 cagtttgtaa attcttcccg ccagagtgaa tgcggttacc tacactccag attactgacc
     3121 actggaggca gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc
     3181 gcaggcaatg tcagccatgt accagcaaga agttccgcaa tatggcacgc tgctggaact
     3241 ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct caactgcacg aaaaaatggt
     3301 aaatgcagac gagctggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac
     3361 tgcacaagag cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag
     3421 ctattacgat ctctcgcagg caggggtgcc ggtacattcg acagcatttc ggcccattga
     3481 tgatgcttct ctggcgcgta atcccttccg cgtttttaca tccttactcc gccttgagct
     3541 tatcgagaac gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt
     3601 caccccacgt tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac
     3661 acaggcacag gagtttgtgc aggaagccct ggaaacgttt cgctggcacc agttagcaac
     3721 ggtagatgaa gaaacctatc gcgccttgca caacgaacat cggttgattg ctgatgtggt
     3781 ctgttttcct ggatgccata tcaaccacct gacgccacgt acgctggata ttgaccgggt
     3841 gcagtcgatg atgcctgaat gcggaattga acccaaaatt ctcatcgagg ggccgccgcg
     3901 ccgcgaggta ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt
     3961 gtttgcgggg cagaaacagg gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg
     4021 cgtggcatta acgccgaaag ggcgacaact gtatgatgat cttctgcgta acgctggaac
     4081 cgggcaggat aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga
     4141 cagtgagttt ttaatgcgtc agcaagggtt ggcatggttc cggtaccgtc tgacgccttc
     4201 gggtgaggcg catcgtcagg cgattcatcc tggagacgat ccacagccct taattgaacg
     4261 tggttgggta gtggcgcaac ccatcaccta tgaagatttc ttgcccgtta gcgcggcggg
     4321 gatcttccag tcaaatctgg gtaatgaaac gcagacacgc agtcacggta atgccagtcg
     4381 cgaagcattt gagcaggcgt tgggttgtcc ggttttggat gagttccagc tttaccagga
     4441 agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg
     4501 caaaaatttg cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc
     4561 cgatttatgc atattctctc agttcaacaa ttggattatt aataaatatt gtctagagtg
     4621 agcggtcata aataagcact ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa
     4681 caccagaagg actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct
     4741 atggccgccg tgtgcggtac cagcggcatt gcttctcttt tttctcaggc agcattcgcg
     4801 gcagattctg atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca
     4861 atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg
     4921 ctggcgacaa tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc
     4981 tggcataacg ttgagaaccg tcaactggac gctcagttct tccatatggg aatgggattc
     5041 cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac
     5101 tttcgcccgg agttgttcaa atacaacgat gcaggtgttg ataccaaaca attagaaggg
     5161 caaagcgatc tcggctttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt
     5221 gatgtagtat catttctcgg cgcgagttat ttccgcgccg ttgatgatac atatcaatac
     5281 ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg acagtaaaga agagttcccc
     5341 gactttaccg ccttctggtt tgatacggta aaaccggggg caactacctt taccgtttat
     5401 gcgttgctcg atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa
     5461 agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg
     5521 ggcatttcgc cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat
     5581 acaattcatc cgcaaattca tgactctgat cgtctgtcca tgtggcgggg caacggcgag
     5641 tggatttgcc gtccgctgaa taatccgcaa aaattgcagt tcaatgctta caccgacaac
     5701 aacccgaaag ggtttggttt attgcaactg gatcgtgact tctcccatta tcaggacatt
     5761 atgggctggt ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag
     5821 ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgc
     5881 ttctggcagc cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg
     5941 tactggagtg cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc
     6001 ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg
     6061 cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc aaaaggcatt
     6121 gagccggtga ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa
     6181 cccatcgatg gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg
     6241 gtcgatatgc ggatgtatct acgttgtcag ggggacgcta tcagtgaaac atggctgtat
     6301 cagtatttcc cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt gatgagttaa
     6361 tcgttttttc ttcggcacct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg
     6421 catttttccc taagttaaat gagtaatctg atggtgtgta tttcagatac accgtgtcag
     6481 ccactaacag ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa
     6541 cgaaaatcct cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc
     6601 cagaaaggaa ggttccgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca
     6661 aaagctacag ttaaaagatg agatgctcaa aatcctgcag caggagagcg tcaaagaggt
     6721 gtaaactttc ctaagccgcc agccaggcgg cttttttaac aacagcatgg attgactgga
     6781 gataagatga ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa
     6841 agtcacgtca cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg
     6901 ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat
     6961 gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt
     7021 atcggcgtta tctcgtttaa tcgtattgaa ccactgaata aaactgctga aatcggctac
     7081 tggctggacg aatctcatca ggggcagggg atcatttctc aggcgctgca gacattgatt
     7141 catcattacg ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat
     7201 ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag
     7261 gctgagttcc tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca
     7321 taataaccct gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc
     7381 acggacatga accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac
     7441 atggttttca attatcaccg cacctgtaat acggctctca ccttgtatta caacgtgctc
     7501 gtcgagcaga atcggtccac cacgtaccac ggcatttccg ccaatcaaga catgatgttt
     7561 taacacacaa ttaccttcca caatggcata ttccgccacc tgcgaactgt aatgaatcgt
     7621 cggaatggca tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca
     7681 atcgcatagc cagacgttgt tctcttcatt accttcaaca ctggcaaaat caaaaacttc
     7741 ggcgcgatgt tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg
     7801 gacaatacgc gaggcgctaa cccttgcgcg atcataaatt tggagcagga gttgatggtc
     7861 gggcgttaag ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa
     7921 tattctacat tgcacgtaaa ctaatgagtc atgaatggtg acactgtcac ttatatatgc
     7981 accctggctg atttcactat tatcgatcca gacgttatcc gttgcgtaaa cctctcccca
     8041 cagcacgctg gtgccggtga tacgcgtatt accggaaatc accgttccgc cgaacgcaat
     8101 agcattctgg tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat
     8161 ccagccgcca gcggttcctg ctatcacatc gttaaaatcg ctaatggcga taatctgccg
     8221 taataacaca ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact
     8281 taggcgatat ttgcgcatag cgttttccca caggtgactt actataaccg tagcaaattc
     8341 tgcggctctg gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa
     8401 ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac
     8461 tttggtattg tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt
     8521 tggcaggtca gccactggtt aggggatggg ctggtgattc tggcgatgat catctgggga
     8581 ttattgacta gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcggaa
     8641 gttcgccatc cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg
     8701 gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt gcatgttcag ttttggtgtc
     8761 gtggttcagt tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct
     8821 gaagaagcta ccacgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca
     8881 atggcctgtg gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt
     8941 ttctcatggc taagccttga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta
     9001 cccacggcac tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt
     9061 gcctggctga gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct tttcggttat
     9121 ggactgctgc aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt
     9181 aatgcttcat tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat
     9241 ctggggagtg gcagcgataa tggatttttc catacgctgg cggtgccgct gtttatcttt
     9301 accaatttta ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa
     9361 ttgttagtca gaaccgagcg cgccgtttta atgaaagcag aggacaaaga atgatcattc
     9421 gtgacgaaaa ctattttact gataaatatg aattaacccg cacacactct gaagtactgg
     9481 aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta
     9541 acagtcttta cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga
     9601 gtatcgccaa cgtcgagcgc attaaatcca ttgaaaatct ggataattta cacacccggg
     9661 tcgttgatct gaataacctc acatttgatg gacagtacga ttttattctt tcgactgtgg
     9721 tgctgatgtt ccttgaggct aaaaccatcc cagggttgat tgccaatatg caacgttgca
     9781 ctaaacctgg tggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta
     9841 ccgtcggctt cacgtttgcc ttcaaagagg gagaattacg tcgatattac gaaggctggg
     9901 agagggtgaa atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc
     9961 gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa atgacccggt aagcacaaaa
    10021 cgcgtgaaaa ttccccacgc tgagatgatt tactgttctt cttttcggta agcatatttt
    10081 ttatcgaagg gatgtgaaat taatcacagt agtcgaagtt ttttagcagc ttaacttact
    10141 gaaatttaac tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa
    10201 aaggggctga aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt
    10261 tttgctataa cttagaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc
    10321 atttgcgaaa gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat
    10381 cacccagcca gataaatata acaattactc tgatttaaaa gaaacaacct cagcaacagg
    10441 taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt
    10501 ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag ggcagaaagt
    10561 tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa
    10621 accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt
    10681 agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc
    10741 ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga
    10801 gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa
    10861 agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga
    10921 catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg
    10981 ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa
    11041 ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga
    11101 tattggcgca cctccgcagc tactggcaaa atagccaggg ctccacaaaa cgcctttgta
    11161 gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg atacgccttt
    11221 gaggctgttt accagactgg atatcgccag tttgggaggt tagttaatta acaaatggac
    11281 gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaataagcac atacatttga
    11341 aaagtaagtg cgtagttttt ctgtcgcgtc atggtc
//
LOCUS       DAJTOW010000118        11184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTOW010000118 DAJTOW010000000
VERSION     DAJTOW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.118"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO244_003365"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO244_003365"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5665076.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO244_003366"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO244_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5665077.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIARAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OO244_003367"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OO244_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5665078.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OO244_003368"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OO244_003368"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5665079.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="OO244_003369"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="OO244_003369"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5665080.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="OO244_003370"
     CDS             complement(5839..6879)
                     /locus_tag="OO244_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5665081.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="OO244_003371"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="OO244_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5665082.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="OO244_003372"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="OO244_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5665083.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="OO244_003373"
     CDS             7821..8261
                     /locus_tag="OO244_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5665084.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="OO244_003374"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="OO244_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5665085.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="OO244_003375"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="OO244_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5665086.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11184
                     /gene="rsxC"
                     /locus_tag="OO244_003376"
     CDS             9490..>11184
                     /gene="rsxC"
                     /locus_tag="OO244_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5665087.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2599 a         2875 c         2816 g         2894 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aactaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccctgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgc
//
LOCUS       DAJTOW010000119        11100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTOW010000119 DAJTOW010000000
VERSION     DAJTOW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.119"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO244_003377"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO244_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5665088.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO244_003378"
     tRNA            complement(621..695)
                     /locus_tag="OO244_003378"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO244_003379"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO244_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO244_003380"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO244_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5665089.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO244_003381"
     CDS             complement(2932..3990)
                     /locus_tag="OO244_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665090.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO244_003382"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO244_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5665091.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO244_003383"
     CDS             6068..6352
                     /locus_tag="OO244_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5665092.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO244_003384"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO244_003384"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5665093.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO244_003385"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO244_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5665094.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO244_003386"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO244_003386"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5665095.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2772 a         2544 c         2745 g         3039 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
//
LOCUS       DAJTOW010000120        11047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTOW010000120 DAJTOW010000000
VERSION     DAJTOW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.120"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            407..1237
                     /locus_tag="OO244_003387"
     CDS             407..1237
                     /locus_tag="OO244_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5665096.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1378..2151)
                     /gene="uxuR"
                     /locus_tag="OO244_003388"
     CDS             complement(1378..2151)
                     /gene="uxuR"
                     /locus_tag="OO244_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5665097.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2366..3826)
                     /locus_tag="OO244_003389"
     CDS             complement(2366..3826)
                     /locus_tag="OO244_003389"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5665098.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3907..5091)
                     /gene="uxuA"
                     /locus_tag="OO244_003390"
     CDS             complement(3907..5091)
                     /gene="uxuA"
                     /locus_tag="OO244_003390"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5665099.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5431..6774
                     /gene="gntP"
                     /locus_tag="OO244_003391"
     CDS             5431..6774
                     /gene="gntP"
                     /locus_tag="OO244_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5665100.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7025..7927)
                     /gene="fimH"
                     /locus_tag="OO244_003392"
     CDS             complement(7025..7927)
                     /gene="fimH"
                     /locus_tag="OO244_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5665101.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7947..8450)
                     /gene="fimG"
                     /locus_tag="OO244_003393"
     CDS             complement(7947..8450)
                     /gene="fimG"
                     /locus_tag="OO244_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5665102.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8463..8993)
                     /gene="fimF"
                     /locus_tag="OO244_003394"
     CDS             complement(8463..8993)
                     /gene="fimF"
                     /locus_tag="OO244_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5665103.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9003..>11047)
                     /gene="fimD"
                     /locus_tag="OO244_003395"
     CDS             complement(9003..>11047)
                     /gene="fimD"
                     /locus_tag="OO244_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5665104.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2684 a         2828 c         2683 g         2852 t
ORIGIN      
        1 gtagatgaaa atacttgtca ccgctgctac ttaattccgg tgggaaatta tttatttccg
       61 cataatcagc tatgtattat ttgaaacaat tagtttgtaa ataatataaa ttgataacat
      121 atcaaataca tcaaaaatac cattcacagg cattattaat atagacctca atatagcaat
      181 atcaaattcc aattagtaat aagatatgct tttaaattgc atgcaaataa ttttaaatga
      241 gcgttttttg atactttaga gtgtttccta ccctttaata tcactgtgta gagttgatat
      301 cactacaaca gaaacatgca tgtcatttgt ttacttctct ttttactaca gaaaaattta
      361 tctatttttc taaacatcag ttaaatactc ataaaaggta actattatgt ctatgccatt
      421 aagcaatgcc gctaataacc aattcaatac aaataatcac ttccttcatc atccaaaagt
      481 cgacagtgag ttaacacgtc gatatgaata tgcacgcctg gatacggaaa atatctatct
      541 tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg tcgaaattag
      601 aaaacttaat attggcaaac agtcctggcc atttaattat gtgacagata catgcagaga
      661 attcaatggt attacaacaa caggacgtat gctctatcga aacttgaaaa tcacctcagc
      721 tttagacgaa atatatggtg gtatatgtaa aaaggcgcat gctgctacag agttagcaga
      781 aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg actatacagt
      841 tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa ccacgatagg
      901 atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc agccaagaat
      961 tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt ccggattcgc
     1021 ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca ttggtataga
     1081 agcctatcct ggtaccgcgg agatccattc aaaaatggga tataacgtta tccccggtga
     1141 tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag aactgtttga
     1201 attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa tgagtactaa
     1261 tattcaggtt atacagatat ttaaacaaaa ccctgcaatt tacaaagtaa tactcattgc
     1321 gacttcttaa tttaagaaag cgcaaggaac gtttaccctt gcgcttatta taataagtca
     1381 ggcgtcgact ttatccagcg gccatgaatc aaagagatag ccgtcaaaat aaatatcgtc
     1441 aacgttcgag aattccagca gacgttgctt aacgttttcc agatgctgcc acattgccag
     1501 cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt gatcgcccaa
     1561 ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc acattggatt
     1621 gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt tatgcgttgc
     1681 ttcagcaatg gcgagatgga actgcatgtc accgctttcg ctgctacccg gcgcgctgga
     1741 agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat cttcgcgggt
     1801 agcctgcagt gcggcaaact cggcgatgtt actttccagt aactgacgcg cctgtaacag
     1861 ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt tgtggctgcc
     1921 tgagctatca agcacataga taccggcacc ccggcgcact tccaccagcc ctttgatctc
     1981 cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt ctgcaatttc
     2041 acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca gatcgtggat
     2101 catcgccccg acttcctggt aaggtctttg cgcagaggtg acagatttca tggtgttgtg
     2161 tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct tatccggcct
     2221 acaacgggca attttacggc ccccgttaat ctcaaactat accattagca ctggaagttg
     2281 gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt tgattgacca
     2341 gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc gcaccgcgtt
     2401 cgcacagctg ctgatatgcc gacgtcaccg cgccaacaaa gtcggcattc tgcggcagat
     2461 catcggcaaa aataccgctc aggcccagca gcgctttcac gcggtctgcg ccctgatact
     2521 gcgcgttaat cttctggaac tcggccagca tcgggtcgac cacgtcaatg gcattaccct
     2581 gctcatccac gccctgggtg taacccatcc agccagccac gcccagcgcc aggtgacgcc
     2641 agctgccgcc gttttgcagg tgcagacgca ccgggtccag tagacgctgc ggtaacttct
     2701 ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag agacgggttg ctgaaacgct
     2761 cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac agcgttggcg
     2821 cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccgggtta gtcatggtgt
     2881 cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag tggctgccgt
     2941 tcagcatacg cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac tgtgtgccca
     3001 ctttatccca gtccgggcga ccattaacga agttatcttc aatcacccac tgacggaacg
     3061 gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt tcctgtaagg
     3121 tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag gtgacgttct
     3181 cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc cagccccagt accgcgacct
     3241 tcgccacatg gccgttttca cgcacgttat cgcaggacat caccgtaaat gctttcagcc
     3301 ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac ttcggcgcag
     3361 tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagttga ccgcttgccg
     3421 catcagcgca gtagcctttt tccgtgaccg ttagagagac aatcgccgtt tgcggacgcg
     3481 ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg atgcagcgct tctttcatcg
     3541 aaccgataat tttcagctcg gtgctttctg cgcctttttc cgctacggtg tacagcagtt
     3601 gctgtttttt caggttttcg atcagcatgc ggtcgttgcc tggcatcagg ttaacttcgc
     3661 agatgcccca gtcgctgtcg gtgctttcca gcagatgatg ggtatacagc gcctggtgcg
     3721 cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga gaatgatccc
     3781 atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta aactccagaa
     3841 tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca gccatgccgg
     3901 tgggtattaa cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc gaacttcggc
     3961 caggcctttc agacgaccaa ttgcggagta accagggttg gttttcttct tcaggtcgtc
     4021 cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc cttccgcttt
     4081 acgacggtgt tcttcttcaa caatcgcttt caccacttcg tacatatcaa cgtcaccgtt
     4141 caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg agcgcagatg
     4201 ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt tgtcagcacg
     4261 cacgccgtag gaaccggtgc acatggtgaa accgttcgcc atgctgttta cggtatcaac
     4321 catccactgc atatcttcaa tggtggaaac aatgcgcggc aagccgagga tcgggcgcgg
     4381 cggatcgtcc gggtgaaccg ccatacgcac gccaacttct tcagcaactg gaataatcgc
     4441 tttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt tgtacagctc
     4501 caggtgctta cggaactggt cgagggtata cccttcttcc gcgcccggca gaccggcaat
     4561 gatgttacgc gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc gttcagctgc
     4621 ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct ggacgtttca ggatatgcat
     4681 ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt ctggcagcac
     4741 gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc acacggtgcg
     4801 aatgccacac tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc actgctcata
     4861 gttgccagtg tgggttttga tatcttcgtg aattggtacg ctttctacga cagaccacac
     4921 caggcctgcg tcttcaacga tcgccttgcg tttgaggatc tcttctacgg accatacttc
     4981 gccgttcggg atatggtgca gcgcggtaac cacgccagtt gcgcccgcct gacggacatc
     5041 agctaaagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca tgatttttcc
     5101 tctttcacat agtgggttcc acagcaaaga gacgcggaac cttggagttg tttaggtatc
     5161 cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg tataagttct
     5221 accttttggt aagacatgtc aaccagaatt ctgaaattgg ttaaccgcgt cgcaagaatt
     5281 tcactctcat ggttgttgct gcaaattgca gcaaagggta atcgatggtt attgatcaga
     5341 ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg cccaaaataa
     5401 caacggttca accaagtaag gtaaaacgtt atgcatgtgc ttaacattct ctgggtggta
     5461 ttcggcattg gcctgatgct gatactgaat ttgaagttca aaatcaattc aatggtggct
     5521 ttgttggtgg cggcgctgtc cgtcgggatg ctggcaggca tggatttgat gtcgctgctg
     5581 cacaccatga aagcgggctt cggcaacacg ctgggagaac tggctatcat cgtggtgttc
     5641 ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat agcacatacg
     5701 ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg tgattatcat cggccttatt
     5761 ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcgccgct ggttattgtt
     5821 attgccgccg aagctaaaat tccgttcctg aaactggcga tcccggcagt agcagctgcc
     5881 actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt gaatgcttat
     5941 ggcgcggata tggggatggt ttatatctat ggcgtactgg tgacgatccc aagtgtaatc
     6001 tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac gccatcattc
     6061 ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt ttcgattctg
     6121 gtgccgctga tcccggcgat cattatgatc tctaccacca tcgccaatat ctggctggta
     6181 aaagataccc ctgcctggga agtggttaac tttattggtt cctcgccgat tgcgatgttt
     6241 attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc gtggtcatga catgcagtgg
     6301 gtgatgaacg cttttgaaag cgcggtgaag agtattgcaa tggtgattct gatcatcggt
     6361 gcgggtggcg tgctgaagca gaccatcatc gacaccggca ttggcgacac catcggcatg
     6421 ttgatgtccc acggcaatat ctcgccctac attatggcat ggctgatcac tgtgctaatt
     6481 cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg ccgccgggat tatcagtgct
     6541 gcaatccttg atccagcaac cggtcagctg gttggcgtga atccggcgct gctggtactg
     6601 gcgacggctg cgggttccaa caccctcacc cacattaatg atgcatcttt ctggttgttc
     6661 aaaggttact ttgacctgtc agtaaaagac acgttgaaaa cctggggtct gctggagctg
     6721 gtcaactccg tggttgggct gattattgtg ctgattatta gcatggtagc gtaaaagaac
     6781 accagagcct gccaatggca ggctcagact gatgactaaa gtctaaaaat gtgcccggac
     6841 agtcccctcg cccctccggg gagagggtta gggtgagggg aacagaccag taaagtgcgc
     6901 tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc
     6961 aggttttgta ggtctgatag cgtagcgcct caggtaccag cattagcaat gcgctgtaat
     7021 ttttttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct
     7081 gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa gttcctactg
     7141 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
     7201 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
     7261 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
     7321 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
     7381 cacaaccgcc agtgggcacc accacatcat tattggcgta aatattccac acaaactgaa
     7441 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgaaccag
     7501 ctttaatagc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
     7561 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat
     7621 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccgtaagccg
     7681 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
     7741 tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta
     7801 cataaacatt agcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg
     7861 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaatagctc
     7921 gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
     7981 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
     8041 tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg
     8101 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     8161 tatccggtgc tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     8221 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacataaga
     8281 ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac cgtacacggt
     8341 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt
     8401 gcgagcgcca acattgccgc caatacatac ccacgtttgc accatttcat gagcatctcc
     8461 agttactgat attcaagagt gaaggtagcc gtagcattga tatgccccgc agtgacaggc
     8521 acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg
     8581 gtcgtccacg aaatcgcaga cgatggagca ttaaggggta tttgattttg ctgctcattc
     8641 agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc aagcaggttg
     8701 gcattgtggc tatctgcaac gccggtaaac ccaactttta cggcagaaac ggcattacca
     8761 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gttgttaaat
     8821 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
     8881 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
     8941 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     9001 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     9061 tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     9121 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
     9181 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg agttagcgtc
     9241 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
     9301 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccaaggt attggtatcc
     9361 agcgccactc tattttcccg atattcagtg gcataaggca gcacggcata accacgccag
     9421 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     9481 agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccaatacc
     9541 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     9601 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
     9661 tcgcctcccc cggcatagcc ggtttgcacg ctataactga ggttgttgtc ttccagcaac
     9721 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag
     9781 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     9841 atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg
     9901 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc
     9961 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
    10021 gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg
    10081 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
    10141 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
    10201 cggtaaccca ctaactgaat attcgtgcct gattcattga gcgatttgtt atagagaaaa
    10261 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
    10321 tccacagaca gagcgcccag tgcctccatg tttttcccga taccgaaatt aaaagcacga
    10381 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
    10441 aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct
    10501 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
    10561 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
    10621 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
    10681 atgccatatc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
    10741 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
    10801 ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc caacgtcagc
    10861 cgggaacgta aaggaattat gtctcgctca atccaggtaa tgatatgctg ccatttattt
    10921 ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc
    10981 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
    11041 atccgat
//
LOCUS       DAJTOW010000121        11029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTOW010000121 DAJTOW010000000
VERSION     DAJTOW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.121"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO244_003396"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO244_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5665105.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO244_003397"
     CDS             complement(1433..1759)
                     /locus_tag="OO244_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5665106.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO244_003398"
     CDS             1894..2235
                     /locus_tag="OO244_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5665107.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO244_003399"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO244_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO244_003400"
     CDS             complement(2833..3543)
                     /locus_tag="OO244_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5665108.1"
                     /translation="MKYKSLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO244_003401"
     CDS             3651..3956
                     /locus_tag="OO244_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5665109.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO244_003402"
     CDS             4155..4475
                     /locus_tag="OO244_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5665110.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO244_003403"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO244_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO244_003404"
     CDS             complement(6299..7288)
                     /locus_tag="OO244_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665111.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO244_003405"
     CDS             complement(7373..7864)
                     /locus_tag="OO244_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665112.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO244_003406"
     CDS             complement(8185..8685)
                     /locus_tag="OO244_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665113.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO244_003407"
     CDS             complement(8675..9190)
                     /locus_tag="OO244_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665114.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO244_003408"
     CDS             complement(9621..9989)
                     /locus_tag="OO244_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665115.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10483..>11029)
                     /locus_tag="OO244_003409"
     CDS             complement(10483..>11029)
                     /locus_tag="OO244_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5665116.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2835 a         2795 c         2734 g         2665 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagtg atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgtactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt ggcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggc tggactggat gtcacggatt aaggcgaccc taggaattaa ggaagtaaat
    10141 ataaagaggg ggaccggatg tgactcacag gttgaaccga atgtcaagga ctaaagacgc
    10201 ctcaggaatt aaggaagtat atataaagag agaccagagg cgactcacag ttaaacagga
    10261 atgtcaagag gagaccatag gaatttggag ggactggagt ttaggacgca gtttaaataa
    10321 gaccacagac agacttagag tcggacagcc gaaggcggac cagtgggcct atagtcagga
    10381 gggattatga ttattatcac cctccctaac ttcagtcaga cttcagtgac ccaaggtctc
    10441 aaccatcaag ccgtatatgg tctccacctt gggtctccct acttaaagta aagctgtctt
    10501 aagcacatca gccagcctac ccacagcgtg tcctttcccg tacaggtcat acgtaattga
    10561 ctgactgctg tgacctaaga tgccttgagc gtccaccaga ttgactccac gctctttcat
    10621 tcgggtcgcc agccaatgtc tcagactgtg gagagagaca tcctgagagt ctcctagggc
    10681 ctcagtcagg acccttgagt taaaccagct agagtaggcg ctcggagtca tcccgaagag
    10741 aggaccctca tcaggctgca tgtctaccca cgacaggaag gacgtcaggt caaacccata
    10801 agcgccatcg gttaacggca caagtcggac tgaatgtttg ttcttcacag acttcccgtc
    10861 cccatcttca ttgatgtcga tacagaccag tccattgtcc aaggttacaa tgtcacgttt
    10921 agtcaggtgg cagacctcag cagaccgagc gccagtgacc acagacacca gagacgccca
    10981 tctacgggcc tcagaggtcg tgtgagccgt gtgtcgtgtg aactgatac
//
LOCUS       DAJTOW010000122        10923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTOW010000122 DAJTOW010000000
VERSION     DAJTOW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.122"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..203
                     /locus_tag="OO244_003410"
     CDS             <1..203
                     /locus_tag="OO244_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665117.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OO244_003411"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OO244_003411"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5665118.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="OO244_003412"
     CDS             2102..2818
                     /locus_tag="OO244_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5665119.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="OO244_003413"
     tRNA            complement(3274..3349)
                     /locus_tag="OO244_003413"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO244_003414"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO244_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="OO244_003415"
     tRNA            5082..5157
                     /locus_tag="OO244_003415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO244_003416"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO244_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5665120.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO244_003417"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO244_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5665121.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="OO244_003418"
     tRNA            7492..7567
                     /locus_tag="OO244_003418"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO244_003419"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO244_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5665122.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9705)
                     /gene="cobT"
                     /locus_tag="OO244_003420"
     CDS             complement(8620..9705)
                     /gene="cobT"
                     /locus_tag="OO244_003420"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5665123.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALVI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRSRRQAEKLLLDVIC
                     YTRELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKID
                     VVRRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALA
                     ACQMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAA
                     CAIYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9717..10460)
                     /gene="cobS"
                     /locus_tag="OO244_003421"
     CDS             complement(9717..10460)
                     /gene="cobS"
                     /locus_tag="OO244_003421"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5665124.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10457..>10923)
                     /gene="cobU"
                     /locus_tag="OO244_003422"
     CDS             complement(10457..>10923)
                     /gene="cobU"
                     /locus_tag="OO244_003422"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5665125.1"
                     /translation="LYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELIHAD
                     INPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAK
                     VVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3037 a         2779 c         2363 g         2744 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcagga
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaagtt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca gacggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccat gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgttggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccggatgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactgcg actcattgcc ggagctgaag caatattgcc
     9301 gctacctcgt gcgacacgca tgttgataag cccggggata ggctcagcag tatcaatacc
     9361 aacatcaatt acgtggacgt tagcgcccgc ttgtgctgcc agcacacaca cgccggttgt
     9421 tccacgggtc atattttcag cctgtatggc tgtcacttct tttggagaaa tagcgacccc
     9481 ttcctcccag acgccgtgat cggcacacat aaccagtacc gcttttttgc ccacatgcgg
     9541 tatgccattc acccccggca ttcctgccag ttgtatgaca agcgcctcca gctttcctag
     9601 gctaccaaca ggtttgaata acccgtcaac atgccgttgt gcacgcgaca tagcggcaga
     9661 attgatggct gggatcgtat tcagtaaatc ggcaattatt tgcatgtcac gtttctttat
     9721 agcagtgcca gcaggaagac cagttcacca agctctatgg ctgctcccag cgtatcgccc
     9781 gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga tgaaaatcgc caccatcgtc
     9841 accaccatag cagccacacc atgcataccc agcaataata cggcagcaaa aatagcggct
     9901 aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc caatgaacac attgccaagc
     9961 ccttcttccc gcgcataacg atgacgatac atcaataatg cggcagtccc gcggctgacc
    10021 gcacatgctg ccgccagcga tgcaaggatc ggttcgccac gcagagccaa ctcgcttaac
    10081 accagaatct ttgccagtac cacaaaaatc aatgccagac cgccgtgggt gcctaaacgg
    10141 ctatcacgca tgatttccaa catgcgatcg cgactacgtg cagaaaatac gccatcacag
    10201 gtatcagcga ggccgtccag atggaattcc ccggtcatca gcgccagcac aagtacgcta
    10261 aacagtgccg ccagtggtgc gccacaccat gcctgcagca ccatgaagac cagcccgcta
    10321 atcgcgccaa gcaataatcc aatcaaagga aaagtaataa taccgcgaga ataatgctcg
    10381 aaatccagtc cctgggacca gcgacgcggt acaggcagac gcgtaataaa tgagagcatc
    10441 gcccaaaata atttgctcat ttgattttta ctccaatacc cgaaaccacc agccatactt
    10501 catttgccgc agcggccaac tgctgattta cccgcccggc aatatcacga aaatgtcgtg
    10561 ccagacgact ctccggcaca atccccattc ccacttcgtt agtcactaat acaacctttg
    10621 cggggcaacg ttggcaggca gcaatcaacg actgaatctc agcattaatc gcctgttcca
    10681 tcgcctgata atcccattca tcagggtctt tatcgccgcc ataatcaaac aacagattag
    10741 tcaccattgt ggtaacgcat tccagcaaca cagcctcatt cgggttaatg tctgcatgaa
    10801 ttaattcatc aagatgttgc cagcgctcca ctgtgcgcca gtgctccggg cggctttgcc
    10861 gatgatgttc tatccgtgca gccatctcat catcaaggat ttgcgaggta gcgatataca
    10921 gaa
//
LOCUS       DAJTOW010000123        10922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTOW010000123 DAJTOW010000000
VERSION     DAJTOW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.123"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            150..500
                     /locus_tag="OO244_003423"
     CDS             150..500
                     /locus_tag="OO244_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5665126.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            553..948
                     /locus_tag="OO244_003424"
     CDS             553..948
                     /locus_tag="OO244_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5665127.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            989..1732
                     /gene="cmoA"
                     /locus_tag="OO244_003425"
     CDS             989..1732
                     /gene="cmoA"
                     /locus_tag="OO244_003425"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5665128.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1729..2700
                     /gene="cmoB"
                     /locus_tag="OO244_003426"
     CDS             1729..2700
                     /gene="cmoB"
                     /locus_tag="OO244_003426"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5665129.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2865..5294)
                     /gene="torZ"
                     /locus_tag="OO244_003427"
     CDS             complement(2865..5294)
                     /gene="torZ"
                     /locus_tag="OO244_003427"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5665130.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5319..6419)
                     /locus_tag="OO244_003428"
     CDS             complement(5319..6419)
                     /locus_tag="OO244_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5665131.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6807..7553)
                     /gene="cutC"
                     /locus_tag="OO244_003429"
     CDS             complement(6807..7553)
                     /gene="cutC"
                     /locus_tag="OO244_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5665132.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7567..8133)
                     /locus_tag="OO244_003430"
     CDS             complement(7567..8133)
                     /locus_tag="OO244_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5665133.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8349..10082
                     /gene="argS"
                     /locus_tag="OO244_003431"
     CDS             8349..10082
                     /gene="argS"
                     /locus_tag="OO244_003431"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5665134.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10259..10747
                     /locus_tag="OO244_003432"
                     /pseudo
     CDS             10259..10747
                     /locus_tag="OO244_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
BASE COUNT         2666 a         2787 c         2607 g         2862 t
ORIGIN      
        1 gggcttttaa tcctatcgtt tattgcttgc atagtagaat ctaaccatcc atcactcata
       61 gctaaccctc ggaaatctta catttctgtc tatcataacc actagcatca cctttaggaa
      121 ccatctgaaa cgggaggtgt atttagacct tgctaatctt agacagccgc ccggttcatg
      181 catcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg
      241 tacatgctgt actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga
      301 cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca
      361 ctacgcctta tcttttttta catacgccag atattgccca ggccccggaa ctggtacata
      421 ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac
      481 agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta
      541 aagggagttt gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag
      601 ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt
      661 tttagcgaac tgcaaagcgc tatttgcatt catggtaacg cggtggaata tattcccatc
      721 gcgattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt
      781 tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg
      841 tttcgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg
      901 gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc
      961 cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc
     1021 tatcgccaga ctgggcgact ggacctttga tgaacgggtc gctgaagtct tcccggatat
     1081 gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga
     1141 gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac
     1201 gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc
     1261 cccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt
     1321 agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct
     1381 gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta
     1441 tcaaggactg aacccgggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc
     1501 caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag
     1561 cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt
     1621 ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca
     1681 gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt
     1741 ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc
     1801 gcgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac
     1861 acggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc
     1921 gccgaaagcg aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg
     1981 aacctgatgc catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa
     2041 tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc
     2101 accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc
     2161 gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg
     2221 cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt
     2281 ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg
     2341 ccgctggagc atctctggca gttgaaagat caactggtga atgagggtga actggtgctg
     2401 gaaacgctgg ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg
     2461 caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag
     2521 tgtggttttg tagatatccg cattgtggat gtgtgcgtta ccactactga agagcagcga
     2581 cgcactgaat ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt
     2641 aagacagttg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa
     2701 aggtctggta ttactgccgg atgcggcgtg aacgccttat ccggcctaca aacccttgct
     2761 aattcaatct attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta
     2821 cgtttgcata acctcagcgc ccgtttccgg gcgctattca cttcttacgc ctgaacagca
     2881 ggcttatcaa atgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc
     2941 gcagagttac cggcacaggc atttgcccgc tgcgagctgg ggatatccgc cgttaacacg
     3001 ttcgcactgc cgtttttaca caagccgttt tccagatctg gccatgcacc ttcatgcacg
     3061 cataccacgc cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt
     3121 ttgttccaga cacgcaccag atcgccattc gcaataccaa atcgagcagc atcttcggtg
     3181 tgaatagtga tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta
     3241 agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg
     3301 gcggtaccct tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag
     3361 ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca
     3421 tttttcaccg gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg
     3481 cggcgcattt cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg
     3541 cgttgcgcac gggccccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca
     3601 tctttacctt cggtatagat ctcttttccg ccaggtttga gtaattccgc aagatcggca
     3661 aacacgtcaa aatcgttacg cgcttcaaat tgcggagcaa cagcctgctt catcggcaca
     3721 atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg
     3781 atcggtaata cgatatctgc gtgtttggct gctgcggtcc agtagcattc agaaacgacg
     3841 atcatctccg gtttctgcca ggctttgatc agacgattag tatcctggtg atgggtaaag
     3901 ttaccgccac ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta
     3961 tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc
     4021 gtcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc
     4081 ggcagcacgc cgccaacacg cgtcgggttg ccaccattgg agtaatgata agagaaacca
     4141 aaaccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag
     4201 tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag cattgtgcga
     4261 ttcgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc
     4321 gccgtccaga ccgcgctctt cggcgtatta tcgctcttac ctgtcagata ctcttcaaac
     4381 tgcggatacc cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc
     4441 agggtatgcg caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag
     4501 gtggcattat cgccaaagaa ttcgatggtt tcggagcgga taggatcaat ggcaatcact
     4561 ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc
     4621 caggcaattt ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt
     4681 tccagaatca gcggccagga ggcctgctgt tcatacacct ctacagaacc gaccacatgc
     4741 ggcatgatca cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc
     4801 gccaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc
     4861 cagccgtaag atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt
     4921 tgctcatgaa tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca
     4981 cgacctttcg ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga
     5041 atacgcgccg tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt
     5101 ttcgccagcg cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gctcatcgct
     5161 ccccaacgtc ccgcggtgag gattttaccg cccttctctt ctgcccatgc ggggagcggt
     5221 gctgccgatg tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt
     5281 cttgttaatg ccataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct
     5341 ttggcgttgt actggaaata ccgcgttaaa atgtccggtt cgttttcgct catgctggtt
     5401 cgtgccccca ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca
     5461 atagaggcat gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc
     5521 ggtttacggt ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat
     5581 tgattgccgt attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct
     5641 tcacttaatg tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg
     5701 ccctgcattt gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg
     5761 gtgagatcgg taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga
     5821 taaatatgtg cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg
     5881 ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag
     5941 gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca
     6001 atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat
     6061 tctttccata ctgtttcggc catttcctgg cgatgagttt cgaacttatc gtcactgtct
     6121 attttgccgc taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa
     6181 taatccatcc ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg
     6241 ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa
     6301 aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg
     6361 ccaccaacca caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata
     6421 gattcctcta aataaattcc agaacaccga aatcattgta taattaaata cacaacgaat
     6481 taaccgcttg cataactcgg cacaacactg cctgaaataa tcggtaaaga atatgattta
     6541 ttacaatgta atcattaatt gctaaggaat aacccagttg ctatttagaa tatatattta
     6601 tcagttttag caatttaaat cccataatca atgtgaatat aaaaaatatt tttagcacaa
     6661 cgctatgcgc actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgct
     6721 cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat
     6781 tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc
     6841 atttcagcaa ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct
     6901 gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg
     6961 gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact
     7021 cctgctccag ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat
     7081 aaaccttgca gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc
     7141 gcaagattat tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc
     7201 actgccagcg gaccggcagc agccattatt ttttccattc gtggcatatc gacattcccg
     7261 tcaacatcga gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca
     7321 tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga
     7381 attatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac ccccaacgac
     7441 ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc
     7501 tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttgct
     7561 ccttaattac gctgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt
     7621 aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc
     7681 ttttggtgaa ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa
     7741 tgccagcgca cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc
     7801 ccaaccttcg tgtgggtaac gtttttcccc gggccacggt agctccacat tggaaaactg
     7861 ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat
     7921 cattttttct gacaaaagct cgccacactg ttcaaaaccg cgacgccagc gctcagcggt
     7981 gacgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga tatccaaccc
     8041 cagacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc
     8101 ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc
     8161 gttaatttac cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca
     8221 atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat
     8281 actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag
     8341 gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg
     8401 caggcgcgcc tgcggattgc gaaccgcaga ttcgtcagtc agcaaaagtt cagttcggcg
     8461 actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat
     8521 tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg
     8581 ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc
     8641 aggcgctggc gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg
     8701 actactctgc gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta
     8761 ttggtgacac agcagtgcgt actctggagt tcctcggtca caaagtgatt cgcgcaaacc
     8821 acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc
     8881 aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa
     8941 agcattacga cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc
     9001 aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc
     9061 agaaccagat cacttacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg
     9121 aaagcctcta caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg
     9181 cagtagaaag cgaaggggcg accgtcgtat tccttgatgt gtttaaaaac aaggaaggcg
     9241 aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata
     9301 tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg
     9361 actcccgtca gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg
     9421 taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca
     9481 aaccgttcaa aacccgcgcg ggtggtaccg tgaaactggc agacctgctg gatgaagccc
     9541 tggagcgtgc acgtcgtctg gtggcagaaa agaacccgga tatgccagct gacgagctgg
     9601 aaaaactggc taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc
     9661 gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc
     9721 catacatgca gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattaacg
     9781 aagagcaact ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg caactggcag
     9841 ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg
     9901 taatgtgtgc ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc
     9961 cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga
    10021 cagcgaagac gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt
    10081 aatcgatttt tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc
    10141 catctaaatg tattctgaaa acggacatgc cattgttttc tcactgttga gtaagaggcc
    10201 agaagcgtaa tatccaggcc caggaaaacg ataacggttc aatttaagga ataccgaagt
    10261 gtttaaattt cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac
    10321 ggttatagta aacgaccatg acacttcagc catcaaagat tgttggcaaa aaaattcaga
    10381 tgatgatact gacattaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga
    10441 tgcgcaatta aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac
    10501 aggtgcaaaa aaacttgata ctgaacaact taacttgctt aaaaaatcac agcgagcctg
    10561 gctggatttt agggacaaag aatgtgaatt aatcctttga aatgaggatg ttcaggattt
    10621 aagtgacccc tattctgaat cagaatggct atcatgtatg atcatacaga ccaatacgcg
    10681 aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc ctttgacaag
    10741 aggataattc acatcttttt ggcatgtttt gttgcaagct attcctgata aatgattgca
    10801 acaaaacatc cagccttttg actgagttat taaacatact cgcgagcgcg taattttttg
    10861 tctttcagcg ataattcaca atcacttcat tccgttgtac ctttagcggt ggaattaacc
    10921 gc
//
LOCUS       DAJTOW010000124        10660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTOW010000124 DAJTOW010000000
VERSION     DAJTOW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.124"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..265)
                     /locus_tag="OO244_003433"
     CDS             complement(<1..265)
                     /locus_tag="OO244_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR5665135.1"
                     /translation="MPAPLYGADAPRRCSGNSVLEVLDKFRKNYDRIMSLPQETKEKK
                     EIRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEYIDPRLRNY"
     gene            complement(282..830)
                     /locus_tag="OO244_003434"
     CDS             complement(282..830)
                     /locus_tag="OO244_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5665136.1"
                     /translation="MNHLMVDLETMGNGPYAPVISIGAVFFDPNTGETGEEFSVNISL
                     ESSMRYRARPDASTILWWMEQSEEARKSLTSNTQELSTALSWLSEFIIKNANHKFVQV
                     WGNGASFDCVILRNSYSLTGQPVPWQWWNDRDVRTIVELGKAIGFDPKRDMPFKGTRH
                     NALDDAIHQAKYVSAIWKKLAK"
     gene            complement(839..1345)
                     /gene="ssb"
                     /locus_tag="OO244_003435"
     CDS             complement(839..1345)
                     /gene="ssb"
                     /locus_tag="OO244_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5665137.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(1346..2053)
                     /locus_tag="OO244_003436"
     CDS             complement(1346..2053)
                     /locus_tag="OO244_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5665138.1"
                     /translation="MNLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQHDWQLMAGNEDAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(2308..2460)
                     /gene="kil"
                     /locus_tag="OO244_003437"
     CDS             complement(2308..2460)
                     /gene="kil"
                     /locus_tag="OO244_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR5665139.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(2445..2576)
                     /locus_tag="OO244_003438"
     CDS             complement(2445..2576)
                     /locus_tag="OO244_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR5665140.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(2601..3569)
                     /locus_tag="OO244_003439"
     CDS             complement(2601..3569)
                     /locus_tag="OO244_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR5665141.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKSKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEEAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRINDEEERRA
                     KDKAHRKEVNNKILADLIKVGASENVAKNIITAIVKGEVFATKITY"
     gene            complement(3756..4205)
                     /locus_tag="OO244_003440"
     CDS             complement(3756..4205)
                     /locus_tag="OO244_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665142.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLIAILTIPLVFWFMKKQCQRVETGKIGDEGYSWERGNPKYRPTMVWDSKGDI
                     NLLIMPLVGVLALWGIFIIGAVTNMTWLKIWLAPKLYLIEYAASLVK"
     gene            complement(4214..4540)
                     /locus_tag="OO244_003441"
     CDS             complement(4214..4540)
                     /locus_tag="OO244_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCR5665143.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLTRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(4905..5309)
                     /locus_tag="OO244_003442"
                     /pseudo
     CDS             complement(4905..5309)
                     /locus_tag="OO244_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5306..5938)
                     /locus_tag="OO244_003443"
     CDS             complement(5306..5938)
                     /locus_tag="OO244_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5665144.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPLTPINGNCKIIGVVVEARVKFV"
     gene            6042..6257
                     /locus_tag="OO244_003444"
     CDS             6042..6257
                     /locus_tag="OO244_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5665145.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            6374..6655
                     /locus_tag="OO244_003445"
     CDS             6374..6655
                     /locus_tag="OO244_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665146.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            6690..6851
                     /locus_tag="OO244_003446"
     CDS             6690..6851
                     /locus_tag="OO244_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665147.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            6838..7668
                     /locus_tag="OO244_003447"
     CDS             6838..7668
                     /locus_tag="OO244_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5665148.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            7665..9041
                     /locus_tag="OO244_003448"
     CDS             7665..9041
                     /locus_tag="OO244_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5665149.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            9114..9320
                     /locus_tag="OO244_003449"
     CDS             9114..9320
                     /locus_tag="OO244_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665150.1"
                     /translation="MAEFMLVACKCVGVGWILLTFFIVLHSYIRLVNDGKDPWYTLFG
                     AAFVWVIIGVMPVVVAKMAWRFVS"
     gene            9338..9613
                     /locus_tag="OO244_003450"
     CDS             9338..9613
                     /locus_tag="OO244_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665151.1"
                     /translation="MDESRKQFLEFAKKQNLSLAYGDCGYVYSSTEMAWRAWQASRAA
                     IEITAPKFIDSREALAKGFTVDYSNGFGDAMDAYEENIRAAGIKVKE"
     gene            9813..10223
                     /locus_tag="OO244_003451"
     CDS             9813..10223
                     /locus_tag="OO244_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5665152.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGKGVRFTDRRYE"
     gene            10220..10402
                     /locus_tag="OO244_003452"
     CDS             10220..10402
                     /locus_tag="OO244_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5665153.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRERARHTR"
     gene            10399..10569
                     /locus_tag="OO244_003453"
     CDS             10399..10569
                     /locus_tag="OO244_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR5665154.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     PNSSMYEEEDDE"
BASE COUNT         2700 a         2501 c         2486 g         2973 t
ORIGIN      
        1 ggtaattacg caggcgcggg tcgatatatt cagggaagtg ggtatatgtt gctttgcgga
       61 acggtcggat tgatgtctgg taaattcgct cgcgttcttc tttctctgca agccatatac
      121 aatggcgaat ttcctttttc tctttcgttt cctgcggtag cgacattatc cggtcgtagt
      181 tttttctgaa tttatccagc acctccaata cggaattgcc ggaacagcgg cggggggcat
      241 ccgcaccata cagaggcgct ggcatgtttt tctcctgttg attatttagc taactttttc
      301 cagatcgctg aaacgtattt ggcttggtga atggcatcat caagcgcgtt gtggcgagtt
      361 cctttgaatg gcatatctcg cttagggtcg aatcctattg ccttcccaag ttcgacgatt
      421 gttcttacgt cgcggtcatt ccaccactgc cacggaactg gctgccctgt cagcgaataa
      481 ctgttgcgga gaataacgca gtcaaatgat gctccattcc cccaaacctg aacgaatttg
      541 tggttagcgt tctttatgat gaattcagat aaccatgaaa gagccgttga aagctcttga
      601 gtgttgctgg ttagcgattt tctggcttct tcactctgtt ccatccacca taaaatcgtt
      661 gaagcgtcag gacgcgcccg atatcgcatt gatgactcaa gcgagatatt taccgagaac
      721 tcttctcctg tttctccggt attcgggtca aagaataccg ccccaataga aataactggc
      781 gcgtatggcc cgttgcccat tgtttcaagg tcaaccatta agtgattcat gtaagtcctt
      841 aaattgcgtg aatagcgtga cgagggaagg ggagagttac tggtgcaaag ggtatatcgt
      901 cgtcaaaatc catcggaggt tcgttgtgtt gtgctggtga tgattgctgc tgtggcttct
      961 gtgattgcct gctggctgct tgttgtttgc tgtcgccaat gccgccaagc atttgcatca
     1021 cgccattaat tccgacatga acctcggttg tgtaacggtc ttgccctgac tggtctttcc
     1081 actttctggt tctcagcatt ccctcgaaat aaatctgatc accttttttc acatactgcc
     1141 ccacgacctc agccagtttc ccggatacag caacacgatg ccattcagtc aattcctttt
     1201 gctcgccagt atttttatct cgccattgtt ctgacgtggc tattgtcagg ttagcgaacg
     1261 ctgttcctga tggtgagtat cgaacttccg ggtcttgtcc tacccgacca aggataatca
     1321 ccttatttac gcctctgctt gccatttatg ccgcctgttt tagttcgtta actctgatgt
     1381 tcattacctg aacgcattta gcctgcgcat cctcattgcc agccattaat tgccagtcat
     1441 gctgataacg ttctatgagt tttttcttgt cagtttctgt tgatgcataa tcgctgaagt
     1501 ctttcaggat ttgttcgcag tcaaccgatg gagatttctg gttggtattt tctggtgatg
     1561 gtttgttatc tgatgctggg attgcccatc ccggcagcga tggagggagc cagtaaaatc
     1621 ctgttccatc cttgagtttt gccctgtgcc atccctgctt tttatcgaga gatgtttgtg
     1681 cgaaaccttc ctcaaggtta tacagatacc gaccgattcc ccactgtacg gcagcgcgct
     1741 tcattgcacc ggaacgacca cctttgacgg cttctacctg cgtgttttca gcagcatccc
     1801 atttggttac ccattcggaa tcaatcttta ttgatatgcc gcattcaacg ccgccgttgt
     1861 tgggaatatc gcggtattca ttgcgccatc ctgctttgcc gcaaacatcg tccaggcgtt
     1921 tcatgattgc ccggttcgtg acataagcca gcaccatagc ccacactttg ccatcgcgtg
     1981 ttttaccgct ttgctgtatt cgccattcga tatcttcagg gctgaatggc tcatcgaatt
     2041 tattcaaatt cataattcac ctcagaatgg acacggccca aggaaataac gctgatttaa
     2101 tacttcgact cgggacaaat taaggcatac ccgcattcct tcgcggtcgc cattatggcg
     2161 ataccagaga gctttctgcg tgtacatgcg tctctgtaac ttgctctcct tcactgtggt
     2221 tgcaagtgac atgaatatct ccttcgttac cgattaattc tttcatctga cgaatgaatt
     2281 cttcgtctga ccagttatct gtaaaactca tttcctgcga taccacggaa ggttgatagc
     2341 tgatttcatc gctttatttg cttcaagcca cattttagaa tcaccaataa atctggctat
     2401 tactgctttg ttttgtgctg cacgaagcat ctggtgattg atggctattt cattgcgcat
     2461 aataagacct caactctttt ccatccgtca cgtaatttac gggtgattcg ttcaagtaaa
     2521 gattcattta gttggaaggc acccatgcga gcgcctcccg cgattgcgta aatcatgggt
     2581 ggttccttat gttggtttta ttagtaggtt atttttgttg cgaatacttc gccttttacg
     2641 atggctgtta tgatattttt agcaacattt tctgatgcgc caaccttgat aaggtcagca
     2701 agtattttgt tatttacttc tttccggtga gctttatcct ttgctctgcg ctcttcttcg
     2761 tcattgattc ttttttcttc tgctattctg gcttgctctt ttgcttcagc ctcgcgacgg
     2821 attcgttcag cctcctcctg tgcttttctg cgttctgctt caattgctgc ctgcttttct
     2881 ctttcagctc gttctgcttc ctcttttgct tcgcgctgtg ctcgttgctc ggcttcaatg
     2941 cgctcacgct ctgcacgctc cgctgcggcc ttagcttctg cttctcgcct tgctgctgct
     3001 tcaatttcgg cttttgcctt tgcttcggct tctgctctgg ctttctcttc agcttctctt
     3061 tttaagcgtt cttcatgctc tcgcttttcc tgctccgctt tgagtcttgc ctcttctctt
     3121 tggcggtcaa attcgcgatc catcaaaatc gctatttcat ggtcagactc aatttgcttt
     3181 gcgagagctt cagctgctgc cttagcttct tcttcggctt taatccgtgc ctgttcctcc
     3241 tcataatcag taagaggctg gcgagccttg gatttcagtt catcaaggcg atcgcgcact
     3301 gtcttgcggt tggcatcaat tagctttgga atttccttca gttcagcaac aaggtctttg
     3361 ccaagaccat cgagatatgt tttcgtctgc gcaactttat acgccagaga agcgatctcc
     3421 tttctgccct ttgccgttgt gatatcaggc acaaaggaca taacttcacg ttcaaccttt
     3481 tgaagaattt cttcaatctg gtcggcagac tgaaatacag tcattgcatt tgctttttca
     3541 ataacaacta aatctgttac ttcactcata tatcctccgt caaaaaattg ccctcacact
     3601 ggagggcaaa gaagattttc aataatcaga acaagtcggc tcctgtttag ttacgagcga
     3661 cattgctccg tgtattcact cgttggaatg aatacacagt gcagtgttta ttctgttgtt
     3721 tatgccaaaa taaaggccga ctatgcggcc tgaaattact taaccaatga tgctgcatat
     3781 tcgataaggt aaagctttgg agccagccaa atttttaacc aagtcatatt ggttactgca
     3841 ccaataataa aaatccccca cagagccaaa actccaacca atggcatgat aagaagatta
     3901 atatcacctt tgctatccca aaccattgtc ggcctgtatt tgggatttcc cctctcccat
     3961 gagtatcctt catcaccgat tttacctgtc tcaactcttt ggcactgctt cttcataaac
     4021 cagaaaacca gtgggattgt tagaatggct attaatgttt taatcagact gtcaaccata
     4081 ttccatagca gcaactgatg aacaacatca ggaatctgtg cctggctaaa tgaaacagcc
     4141 gcatctattc cattgctggc tttttgcagt agttctacga gaatcttgtt tgcttgttct
     4201 tccatatctc acctcaaata agtggtttgc tgcctaattt cattttctgg cgaccaacac
     4261 aagtcacacc catttcactg cgtggcttgc tgtaccatgt gcgctgattc ttgcgctcaa
     4321 tacgttgcag gttgctttca atctgttcgt ggtattcagc cagcaccgta aggtctatcg
     4381 gattcagtgc gctttctact cgtgatttcg gtttgcgagt cagcgagaga atagggcggt
     4441 taactggttt tgcgcttacc ccaaccaaca ggggatttgc tgctttccat tgagcctgtt
     4501 tctctgcgcg acgttcgcgg cggcgtgttt gtgcatccat cttgattctc ctgtcagtta
     4561 gctttggtgg tgtggtaggt gggagaccca tttcgacctg cttcggccga cttcaattcg
     4621 gcaatagttc cgcaggcctc gccgctttac gtgcgacata ttcccatcca tgaacccttc
     4681 accacacccc aaagccttct gctttgaatg ctgcccttct tcagggctta atttttaaga
     4741 gcctcacctt catggtggtc agtgcgtcct gctgatggct taaaactaca agaaagattg
     4801 tatgctgtaa acaagaaata ttgtaaaaag gggcgtaaaa aacaaactcc attgttttta
     4861 aatgaaaaat agtttgtttt tggttatcga gattgaggtg gggattactg gttgcaggtt
     4921 ccgactacat caccaacaaa tgatttggtt gatgtaagtt gttgcatacc tgggatattc
     4981 attactttgg agtaaagagc ttttttgtct gtagtgattg atcaagtttc aacggttatt
     5041 cctcctccag actggtattc tcctaccata gtgttcgatg acaaagcagt gtatttcatc
     5101 tctggataga cgccagaaac tgattcataa actgatgatt tatcgccatt tattgttacg
     5161 tggaaaacgg aatcttccgt gctgtctttt gtaaactcgt aacgatcgcc attcattgac
     5221 ccgtacccgt gcaggtttgt gacaatccag cattcagaat tggcgctggt agttaagagt
     5281 attgagagta gcgccgcaat cctgatcata cgaattttac cctcgcttcc acgacaacac
     5341 cgataatctt gcagttcccg ttgataggag tcagaggcca tgaagggttc aggcctttca
     5401 ggtacttctg cccgccatct ataaccagtt tcttgaatgt tgcttcgttc gcgtcagtca
     5461 gtttggctac aacaaggctt ccattcactg gctcgcgtcc agtatctact aacaccatat
     5521 gaccttcagg gatgctttga cctacaggtg aggtcatgga atcaccttca accttcagcc
     5581 agaatccatc gcctaataag ttaacgtcac tgtcatacca ttcatcaatg tccttgatat
     5641 cgtagggttc acaagcttca caccacgaac cagctctaac catgctaatc aatggatatt
     5701 tccctttggg ctcaacatgc ccaacaaatc taacattcga atcagaggtg ccattgagca
     5761 gccagtcaac acttacgcca agagctgacg caagttctgg taaaaagcgt ggtcgcttag
     5821 ttttaccgtt ttcgagctgc tctatagact gctgggtagt ccccaccttt tgagcaagtt
     5881 cagcctggtt aagtccaagc tgaattcttt tgctttttac cctggaagaa atactcataa
     5941 gccacctctg ttatttacct ccaatcttca caagaaaaac tgtatttgac aaacaagata
     6001 cattgtatga aaatacaaga aagtttgttg atggaggcga tatgcaaact ctttctgaac
     6061 gcctcaagaa gaggcgaatt gcgttaaaaa tgacgcaaac cgaactggca accaaagccg
     6121 gtgttaaaca gcaatcaatt caactgattg aagctggagt aaccaagcga ccgcgcttct
     6181 tgtttgagat tgctatggcg cttaactgtg atccggtttg gttacagtac ggaactaaac
     6241 gcggtaaagc cgcttaagac attcccgctc ttacacatcc cagccctgaa aaagggcatt
     6301 accagaaaca aatctctatg gttttgcgtt tctttgcgaa gccaactcta tctaatcatt
     6361 aaggaaatta tctatgggta ctattgcaac taaaagcaag aaagcggctc gcatcgagtc
     6421 agccttgctg aacaaactgg cactgatggg gcagaagaca ttcgctcgag caatgggggt
     6481 tcctgaatat caggtaagcc gatggaagaa tggtttcttc tcgcaggtaa gcatgatgct
     6541 tgctgttctg gaatacggaa tcgaagacga tgaaatggct gaattgacta agcggcttgc
     6601 cgattacctg acaaaagaaa aagccccgaa gaacggcgaa ttcttcgagg cctaatgtag
     6661 aaagactgga tcaatccacg ggagtaatta tgacaaaacg tcgtaagaaa taccaggaaa
     6721 aagaagagat tcgacaccct gattcacctg agggattagt ggtagccgca gcaaataaca
     6781 gggcgttcgc agagcgcctt gttggtgttt acagactagc caaagcagga gtgaaacatg
     6841 ggcgtcgtta agttagctga ttacaggcct caactggagg tcgtggagca tcgcgtggca
     6901 gaactcgaag atggctacac tcggactgca aacacactgt tagaagccgc catgctttct
     6961 ggacttactc tacatcagtt actgattgtt atggctgtgt ggcgcaagac atacggttac
     7021 aacaaaaaaa tagattggat cggaaacgaa cagttcgctg aactcactgg catggcacca
     7081 accaaatgct ctaccgccaa aaacgagctt atcagaatgg gagttctcat tcaggtgggg
     7141 cgtcaagttg gtatgaatac aaacatttcc gagtggaaaa cgaaagttaa cggattcggt
     7201 aaaacattta ccaattcggt aaaacaaacc ttcaccaaat cggtaaaaag caatttaccg
     7261 aatcagtcaa acacaaaaga caatatacaa aagacaataa atacaaatac ccccttaccc
     7321 cctaacgggg gcggcgatgg gcaggttaaa cctgaacgtc gcaaggcaga acgaatcgac
     7381 tacgaatcct tcctgaacgc ctacaacacc gaagtcggtg acagactgcc acacgctgtt
     7441 gcggtcaacg agaaacgcaa acgccgcctg aagaaaatca tcccgcaact gaaaacgcca
     7501 aacgtggacg gtttcagagc gtatgtcagg gcgtttgtgc atcaggccaa gccgttttac
     7561 ttcggagaca acgacacggg ctggacggcc gattttgatt acctgctgag ggaagactcg
     7621 ttaacggggg tacgggaagg gaagtttgca gacaggggga ttgcatgaaa caggatatcg
     7681 aagcgagcgt tatcggtggc ctgctgattg gtggattaac accaaccgcc agcgacgttc
     7741 tggcaacgct ggagccggaa gcgttttcaa ttccgctcta ccggaaagcc ttcgaggtta
     7801 tccgcaagca ggcgcgaaac agaaacctaa tcgacgcgct gatggttgcc gaggcgtgcg
     7861 gagaggagca tttcacgtca atcctgatga ccagcaagaa ctgcccgagt gccgcaaacc
     7921 tgaagggata tgctggaatg gtcgcggata actatcaccg ccgtctggtg ctggaaatca
     7981 tggatgaaat gcgtgaacca attcagagcg gaaccatcga cgcatcgagt caggcgatgg
     8041 atgagcttgt aaagcgtctt tcagccatca gaaagccccg tgacgaagtt aaacctgtac
     8101 ggttagggga aatcattact gactacactg acacgcttga caggcgtctg aggaacggag
     8161 aagagtccga taccctgaag accggaatcg aagaacttga tgccatcacc ggagggatga
     8221 acgcggaaga cctggtgata atcgctgctc gtcctggtat ggggaaaacc gaactggcgc
     8281 tgaagattgc cgaaggcgtt gcaagccgcg ttattcctgg ttctgacgtc cggcgcggag
     8341 tgttgatttt ctcaatggaa atgagcgcat tgcagattgc agagcgaagc attgccaacg
     8401 ccgggaggat gtcggttagc gtgctacgaa atcctgcatc gatggatgac gaaggctggg
     8461 cgcgtgttgc taacggcatg agtcagcttg cagatttgga tgtatgggta gtcgatgcct
     8521 cgcggttatc ggtcgaagaa atacgctcaa tcgcagaacg gcacaaacag gaaaatccaa
     8581 acctgtcact cattatggcg gattatcttg gcctgattga gaagccgaaa gcagaccgca
     8641 acgacctcgc aattgctcac atctccggaa gcctgaaggc gatggcgaaa gacctgaaaa
     8701 cgcctgttat ctccctgagt cagctttcgc gcgatgttga gaagcgacca aacaaacgcc
     8761 cgacaaacgc agatttgcgt gattcaggaa gcattgaaca ggacgcagac tcaatcatca
     8821 tgctctatcg ggaagcggta tatgacgaga acagtagcgc cgcaccattt gctgaaatca
     8881 ttgtgacaaa aaaccgtttt ggctcgcttg gtacggttta ccagcggttc tgcaacggac
     8941 actttgttgc atgtgaccag gatgaagcca gacagatttg cacagcatca aatgcgcccg
     9001 ctgcgcgtgg cagacgatat gcacaagggg ctgacgtatg accatctacg tcactgagct
     9061 aataacaggc ctgctggtaa tcgcaggcct ttttatttgg gggagagtaa atcgtggctg
     9121 agtttatgct cgtcgcatgc aaatgcgttg gcgttggatg gattcttctg acgtttttta
     9181 ttgttctgca tagctacatt cgtcttgtga atgacggtaa agacccatgg tatacgttgt
     9241 ttggcgctgc atttgtctgg gtgattatcg gtgttatgcc tgttgtcgta gcaaaaatgg
     9301 cgtggcgttt tgttagttga actgagggta agtatcgatg gacgaatcaa gaaagcagtt
     9361 tttggagttc gcgaaaaagc aaaatttaag ccttgcatat ggtgattgcg ggtatgttta
     9421 ttcatccacg gaaatggcat ggagggcatg gcaggcatct cgcgcagcta ttgaaataac
     9481 cgcgccaaag tttatcgaca gcagagaagc attagccaaa gggtttactg ttgattattc
     9541 caatggcttc ggtgatgcaa tggatgctta tgaggaaaac atccgcgctg ctggaatcaa
     9601 agtgaaggag tgagcatgag tcgacgaagt agctttttgg ggtttgtaat attcctgtcc
     9661 tgcactggtt acatcgtaat ctggtcaatt tcgaacattg accgtggcgg ggaatatctc
     9721 attgtaatgt tctttccttt gtttcttggg tggtacgccg caaggttgct ggaagaatgg
     9781 ggttacaggc ataaaaaata aaggagtatt cagtgaagca aacaatcttc ctccgaacta
     9841 agcaacaaca gcaagctgca ataaatgcca tcctcgcaac accactcgat aaagacaagc
     9901 cagtcaccat ccgcattact gactacaagc gcaaccttga ccagaacgca aaatttcacg
     9961 cgatgctggc ggatatcgca cgtcaggttc aatggtgcga taaatggtta aaaccagaac
    10021 aatggaaggt tttgttgata agcggtcatg cagtggcgac aaagcaggaa gctgatgttt
    10081 tgcccgggct tgaaggcgaa tacgtcaaca ttcgtgagag cagcgcacag atgagcgtga
    10141 agcgtatggc aagtctgatt gagtacacga cagcatgggc tattggtaag ggtgtcagat
    10201 ttaccgacag gaggtacgaa tgagacgaca gcgacgaagt atcaccgaca taatctgcga
    10261 aaactgcaaa taccttccaa cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga
    10321 atctgacgta aaaaccttca actacacggc tcacctgtgg gatatccggt ggcttagaga
    10381 acgtgcgagg catacaaggt gattgaccca aatcgaagtt acgaacaaga aagcgtcgag
    10441 cgagctttaa cgtgcgctaa ctgcggtcag aagctgcatg tgctggaagt tcacgtatgt
    10501 gagcactgct gcgcagaact gatgagcgat ccgaatagct caatgtacga ggaagaagac
    10561 gatgagtgat ttctctgagc ttatttcctt caaaaaagac agagaagaaa tgcggactga
    10621 atctgtctat tacgttcaac accggaataa acgctctgtg
//
LOCUS       DAJTOW010000125        10465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTOW010000125 DAJTOW010000000
VERSION     DAJTOW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.125"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            4..204
                     /gene="cspG"
                     /locus_tag="OO244_003454"
     CDS             4..204
                     /gene="cspG"
                     /locus_tag="OO244_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5665155.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            215..403
                     /locus_tag="OO244_003455"
     CDS             215..403
                     /locus_tag="OO244_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5665156.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            378..608
                     /gene="ymcE"
                     /locus_tag="OO244_003456"
     CDS             378..608
                     /gene="ymcE"
                     /locus_tag="OO244_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5665157.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            598..771
                     /locus_tag="OO244_003457"
     CDS             598..771
                     /locus_tag="OO244_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5665158.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(820..1893)
                     /locus_tag="OO244_003458"
     CDS             complement(820..1893)
                     /locus_tag="OO244_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5665159.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1995..4706)
                     /gene="torS"
                     /locus_tag="OO244_003459"
                     /pseudo
     CDS             complement(1995..4706)
                     /gene="torS"
                     /locus_tag="OO244_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4789..5817
                     /gene="torT"
                     /locus_tag="OO244_003460"
     CDS             4789..5817
                     /gene="torT"
                     /locus_tag="OO244_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5665160.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYCVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5790..6482)
                     /gene="torR"
                     /locus_tag="OO244_003461"
     CDS             complement(5790..6482)
                     /gene="torR"
                     /locus_tag="OO244_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5665161.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6569..7741
                     /gene="torC"
                     /locus_tag="OO244_003462"
     CDS             6569..7741
                     /gene="torC"
                     /locus_tag="OO244_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5665162.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7741..10281
                     /gene="torA"
                     /locus_tag="OO244_003463"
                     /pseudo
     CDS             7741..10281
                     /gene="torA"
                     /locus_tag="OO244_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10278..>10465
                     /gene="torD"
                     /locus_tag="OO244_003464"
     CDS             10278..>10465
                     /gene="torD"
                     /locus_tag="OO244_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5665163.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2683 c         2788 g         2469 t
ORIGIN      
        1 aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct
       61 gatgatggca gcaaagacgt tttcgtccat ttcaccgcca tccagagcaa tgaattccgc
      121 acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg
      181 gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg
      241 tccatgttgt catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc
      301 tttgggcgct aagtgtattt tttgtaaatc gactatgatc acctttgata acgtcgcgct
      361 gcaaatacgc actgaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg
      421 gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc
      481 tgttgattat tgaatctttc gggaagatgg cttataacat ttcttacctg accagggtac
      541 cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagacgag gttaacagtg
      601 aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa
      661 atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt catcgcacga
      721 gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa
      781 catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
      841 ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata ctcccgcata
      901 cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc
      961 agacaacgca aaggcttttt tatttgctgc aggtttttgc agtgaaaagg tcaatgttga
     1021 ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
     1081 aattaattta tctaccggga tccgtgatgg gcactttttt gcgcatttgc cacaatcgat
     1141 acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag
     1201 cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag
     1261 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
     1321 aacgtccatc accacgctat agggcgacaa cataaaatag tgaatcgcct gagcgggcat
     1381 caataacgcg atatagagaa ataaactcaa cagcaaatac ttcacgccgc gcagaggaat
     1441 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
     1501 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
     1561 actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg
     1621 acctgttgca aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat
     1681 acctcccggt ctggcgacaa atgtcgtact acctgccgtt tcgtaatagc gcacccaata
     1741 ccagaacgtg atggcaatat aaatattcat tgccaatagt aagaattgcg tcgctttacg
     1801 ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg
     1861 ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata
     1921 ccaaatgcca ctattctagt tattcttaac tggctgatat tgattcaaat cgcgttcagg
     1981 tctttcttat tcaatcatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtgga
     2041 aggggggcag ataatggctg ttgctccagt tgtgcgcata gctgactggc gctacgcatt
     2101 cccagactcg agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctgtcc
     2161 tggctggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tttaaataat
     2221 gccagccatt cgtggatctt ctccgtcccc attaactgag cgtcttcatt gagttgcgag
     2281 acatccagtg gtagatcgtt attggcttgc agttggagat agtgcgccag taactgaccg
     2341 agcacttcac gcggcaccgg tttagggata atcccgcgaa atagtgaact ggtacgctgg
     2401 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
     2461 tgtgccagtt gccgggcaag ggtaatgccg tcgatatccg gcagatcaaa atccaccagt
     2521 gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaata
     2581 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
     2641 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt ggctgttttt
     2701 ggcacgggtg cagtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
     2761 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
     2821 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
     2881 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
     2941 ccatcagtgc gactacgcag gataatgtgc ccttcgtcag taaaacgcag ggcattgctc
     3001 aataggttgg taatgacctg acgaatacgt cgtggatcgc ccattaacgc gctcggtaca
     3061 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
     3121 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac tgaaacattt
     3181 ttgccacctg cttcgatagc cgaataatcg agaatatcgt tgaggatggt cagcaacgat
     3241 tcgccagagt cggtaatcgc ctgcaaatca tcacgctggg cgttaagtgc ggggttatct
     3301 gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
     3361 gccgtcagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
     3421 tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
     3481 tcgcggtggc gattcagtgc atgaacactg ctgcgaaacg catccatcag ccgcccgatg
     3541 gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
     3601 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgaatgagcg ataaaccacg
     3661 cgccagagga tcataatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat
     3721 tgcccgcgtg cactggcttt ttccagatgt gccagtccat gctgattgtg cagctcaatg
     3781 gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat attattttgg
     3841 gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc caacagatcg
     3901 ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaactcc cggatcttca
     3961 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
     4021 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
     4081 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
     4141 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
     4201 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgctccag gcgtgcgatc
     4261 tcatcggcgg cagctatctg ctgactgagt tgccgttgtt gctggcgtag ttgcagacgc
     4321 cgccccacca gttccccttg ctgacgtaat gaacgggaga tctcctgctc ctgttgttca
     4381 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
     4441 ctttatgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
     4501 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
     4561 atggtaggaa tcaatgcctg agtattgtct ttttccacct ggctgataaa gcgcaggttg
     4621 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
     4681 agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg
     4741 caaagtggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
     4801 ctatttttac ttctttccct tttcatgttg tcggcatttt cggctgataa cctgttgcgc
     4861 tggcatgatg cgcagcattt cacggtgcaa gcctctatgc cgcttaaagc caaacgcgta
     4921 tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat
     4981 ggtatgcagg aggctgctcg ccgctactgt gtggatttaa aagtgctgga ggcaggcggc
     5041 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
     5101 gccattttgc tcggtagtag cacgacgtca tttcccgacc tgcaaaagca ggtagcaaat
     5161 ctgccggtga tcgaactggt aaatgctctt gatgatcccc aggtgaaaag ccgcgttggt
     5221 gtaccctggt ttcagatggg ctatcaacct gggcgatatt tggtgcaatg ggcgcacggt
     5281 aaaccactga acgtgctgct gatgcccgga cccgataacg ccgggggcag taaggagatg
     5341 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
     5401 ggtgataacg atattgaaat ccagcgcaac ctgttgcagg agatgctgga acgccaccca
     5461 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg tgaagggcgt
     5521 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
     5581 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
     5641 gcagttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
     5701 ccgattttag ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg ctcgctgtca
     5761 ccggggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct
     5821 tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg acgaattaac
     5881 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgcagcaga
     5941 cgctcacggc tgagaatttc gcccggatta gtcacaaatg ccaccaacat ttcatactct
     6001 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
     6061 caataacccg caaagcgata gcagtagtcc tgagtgtgcg gttgagcttg tcgcgcgagg
     6121 tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg
     6181 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
     6241 ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg
     6301 ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccggctg attctgcata
     6361 atttcccgta gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
     6421 taggattgta atcgcgcctg ggtaaccggc ttatcttcaa caataacaat gtgatgtggc
     6481 atcagagggt tttactcatt ctgttcatat ctgttcatat ttgctcatta agatcgcttc
     6541 actaaaccat aattctacag gggttattat gcggaaactc tggaacgcgc tacgccgacc
     6601 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6661 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6721 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6781 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6841 gcgcaaactg gaagcgagca acgacattta ccagaccttt attgcccact ccattgatac
     6901 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6961 agaaaacaac tcggcaacct gtcgctcctg ccataactac gatgcgatgg atcatgccag
     7021 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     7081 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     7141 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     7201 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     7261 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     7321 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     7381 ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacca ccgttgccga
     7441 caccaatacc gagtggagca agttgcaggc caccgcgtgg atgaagaaag gcgacatggt
     7501 gaacgatatc aaaccgatct gggcctatgc ggattcgttg tacaacggca cctgtaacga
     7561 gtgccacggc gcaccggaaa tcgcccactt tgacgctaac ggttggatcg gcacgctcaa
     7621 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcactttgt tgaaatatct
     7681 gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7741 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7801 ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc aactgcggcg
     7861 caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc gcactggggg
     7921 gctatccgcg cgatggtgaa ggatggtcgc tttgtggtgg caaaaccgtt cgaactggat
     7981 aaatatccgg cgaaaatgat cgccggattg ccggatcacg tacacaacgc ggcacgtatt
     8041 cgttatccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga cacctcccag
     8101 cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat gttctatgaa
     8161 gaactggaac gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc cagtggttgg
     8221 caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat tgccttgcat
     8281 ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt gatcctgccg
     8341 cgcgtagtcg gttcaatgga agtgtatgaa cagcaaacct cctggccgct ggtattgcag
     8401 aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca agcgaactgg
     8461 tggtgcccgg atcacgatgt ttatgaatat tacgagcagc taaaagcgaa agtcgccgcc
     8521 ggtgaagttg aggtcatcag catcgatccg gttgtcacat ccacccatga gtatctgggg
     8581 cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct gcaactggcg
     8641 ctggcacata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc taactactgt
     8701 gtgggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca gccgaaagat
     8761 gccgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg gctggcgcgg
     8821 cagatggcgg cgaacagaac gcaaattatt gctggctggt gcgtgcagcg tatgcagcac
     8881 ggtgaacagt gggcgtggat gattgtggtt ctggcggcga tgctggggca aattggcctg
     8941 ccaggtggtg gttttggctg gcactacaac ggcgcaggca cgccggggcg taaaggcgtt
     9001 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
     9061 aaaggctaca gcagcactat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     9121 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
     9181 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     9241 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
     9301 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
     9361 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgtagttcga ggcgcgcaac
     9421 gactttgata ttttccgcga gctgtgccgt cgctttaatc gtgaagaagc ctttaccgaa
     9481 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
     9541 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
     9601 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
     9661 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
     9721 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
     9781 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
     9841 tcgcagctat gtgagtcgga aacgctgcgt cagcaatata cggtagcggg taaagagcca
     9901 gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
     9961 gtctttaacg ctcgcggtca ggtgttggca ggagcagtag tttctgaccg ctatgcaccc
    10021 ggcgtggcgc gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
    10081 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcgggac ttcgcagctg
    10141 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggaacagtg
    10201 gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga atatgttccc
    10261 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
    10321 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    10381 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctcact gcggcggtga
    10441 atgatctgga aaacagtatt gccac
//
LOCUS       DAJTOW010000126        10142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTOW010000126 DAJTOW010000000
VERSION     DAJTOW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.126"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..330)
                     /locus_tag="OO244_003465"
     CDS             complement(<1..330)
                     /locus_tag="OO244_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5665164.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEA"
     gene            762..1880
                     /locus_tag="OO244_003466"
     CDS             762..1880
                     /locus_tag="OO244_003466"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5665165.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1946..2194
                     /locus_tag="OO244_003467"
     CDS             1946..2194
                     /locus_tag="OO244_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5665166.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2259..2627
                     /locus_tag="OO244_003468"
     CDS             2259..2627
                     /locus_tag="OO244_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5665167.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2721..3374
                     /gene="nfsB"
                     /locus_tag="OO244_003469"
     CDS             2721..3374
                     /gene="nfsB"
                     /locus_tag="OO244_003469"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5665168.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3482..4729
                     /gene="ybdG"
                     /locus_tag="OO244_003470"
     CDS             3482..4729
                     /gene="ybdG"
                     /locus_tag="OO244_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5665169.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4797..6173)
                     /gene="pheP"
                     /locus_tag="OO244_003471"
     CDS             complement(4797..6173)
                     /gene="pheP"
                     /locus_tag="OO244_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5665170.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6274..9417)
                     /gene="cusA"
                     /locus_tag="OO244_003472"
     CDS             complement(6274..9417)
                     /gene="cusA"
                     /locus_tag="OO244_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5665171.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAVLLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNLQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9429..9947)
                     /gene="cusB"
                     /locus_tag="OO244_003473"
                     /pseudo
     CDS             complement(9429..9947)
                     /gene="cusB"
                     /locus_tag="OO244_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2490 a         2800 c         2452 g         2400 t
ORIGIN      
        1 agcttctccg cgctcttcat agaccttcag ccacctggct acagaaccac taccagcaag
       61 cataaagtga gcagcagcct gattaaggga catgtgctgc tcgatcacag ctttcacgac
      121 cttaatacgc aactctggat cagcactaac gcctttaggt ttgggaatta aacctttttc
      181 tccatgtttt tcatagaggg caacccatgt cctgacctgg gttcggggga caccaaaacg
      241 tgccgagatg atcctgtaac catcatcagt agtgaagtag tgattcacga cttcaaggcg
      301 cttttcaaaa gggtattttg gctttgacat attaggggct attccatttc atcgtccaac
      361 aaaatgggtg cagtacaggg ttgatagaaa tatctcctgg ggcttcatct ttccagacct
      421 cagagtaaac ctgaaatcgg aaagcttcag gcacccgcag ttatcttacc cttcctcaat
      481 aaaatagctg tttatttata cagcaaatcc tgtttcggac ttctcactcc ccatttgatc
      541 aactgaagct gaacccgttt ttttctgcat gcctcaatcg tcaaaattct tcgccaccag
      601 ccagctctgc cataacttgc cacattgaaa aagtagccaa agtgcttcag gcaccatcag
      661 ttcaccatca ccaaaaaaaa gaaacattcc agaacttccg catctcgccc agccgctccg
      721 acttctatac tgaatagaaa acgtcaactt aagagaaacc tatgccatta cccgattttc
      781 atgtttctga accttttacc ctcggtattg aactggaaat gcaggtggtt aatccgccgg
      841 gctatgactt aagccaggac tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg
      901 ccggagaggt aaagcacgat atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc
      961 gtgatatcaa ccaggctgcc gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag
     1021 ccgcagacca tcatctggaa atttgcggcg gtggcacgcc cccgtttcag aaatggcagc
     1081 gtcaggaggt atgcgacaac gaacgctatc aacgaacgct ggaaaacttt ggctatctca
     1141 tccagcaggc gaccgttttt ggtcagcatg tccatgttgg ctgtgccagt ggcgatgacg
     1201 ccatttattt gctgcacggc ttgtcacggt ttgtgccgca ctttattgcc ctttccgccg
     1261 cgtcgccata tatgcaggga acggatacgc gttttgcccc ctcacgaccg aatatttttt
     1321 ccgcctttcc tgataatggc ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc
     1381 tgtttcgctg tctgagttac accacgatga tcgacagcat taaagatctg cactgggata
     1441 ttcgccccag tcctcatttt ggcacggtgg aggttcgggt gatggatacc ccgttaaccc
     1501 ttagccacgc ggtaaatatg gcgggattaa ttcaggccac cgcccactgg ttactgacgg
     1561 aacgcccgtt caaacatcag gagaaagatt acctgctgta taaattcaac cgtttccagg
     1621 cctgtcgcta tggtctggaa ggcgtcatta ccgatccgca cactggcgat cgtcgaccgc
     1681 taacggaaga taccttgcga ttgctggaaa aaatcgcccc ttcagcacat aaaattggcg
     1741 catcgagcgc aattgaagca ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc
     1801 tgatgcgcga tttcgtcgcc gatggcggct cgctgattgg gctggtgaaa aagcattgtg
     1861 agatctgggc cggtgactaa gccgggaatt gccttacaga agaatatccc tgacaatggt
     1921 gttttaactt acatttaaca atgttatgaa acacccttta gaaaccttga ccaccgcagc
     1981 aggcattttg ctgatggctt tcctctcttg cctgctgctg cccgcccccg cactggggct
     2041 gacgctggca caaaaactgg tgaccatgtt ccatctgatg gatcttagcc agctttacac
     2101 tttattgttt tgtctgtggt ttttagtgct gggcgctatt gagtattttg ttctgcgctt
     2161 tatctggcga cgctggttct cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg
     2221 catactttgc ttattctcct tcgcctaaag gaacgtttat ggataagcaa tcactgcacg
     2281 aaacggcgaa acgcctagct cttgagttac cctttgtcga gctttgctgg ccttttggcc
     2341 cggagttcga tgtttttaaa attggcggca agatttttat gctgtcgtcg gagctacgcg
     2401 gcgtcccctt tatcaatctg aagtccgatc cacaaaaatc cctgttaaat cagcaaatat
     2461 acccaagcat taagccaggg tatcacatga ataaaaagca ctggatttcg gtgtattccg
     2521 gcgaggaaat ctccgaagcg ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg
     2581 ttgatggtct ggctaaacgc gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat
     2641 agcgcatttt tctcgcttac catttctcgt tgaaccttgt aatctgctgg cacgcaaaat
     2701 tacttacaca tggagtcttt atggatatca tttctgtcgc cttaaagcgt cattccacta
     2761 aggcatttga tgccagcaaa aaacttaccc cggaacaggc cgagcagatc aaaacgctac
     2821 tgcaatacag cccatccagc accaactccc agccgtggca ttttattgtt gccagcacgg
     2881 aagaaggtaa agcgcgtgtt gccaaatccg ctgccggtaa ttatgtgttc aacaaacgta
     2941 aaatacttga tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct
     3001 ggctgaagct ggttgttgac caggaagatg ccgatggccg ctttgccacg ccggaagcga
     3061 aagccgcgaa cgataaaggt cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg
     3121 atgatgcaga gtggatggca aaacaggttt atctcaacgt cggtaacttc ctgctcggcg
     3181 tggcggcgct gggtctggac gcggtgccca tcgaaggttt tgacgccgca atcctcgatg
     3241 cagaatttgg tctgaaagag aaaggctaca ccagtctggt ggttgttccg gtaggtcatc
     3301 acagcgttga agattttaac gctacgctgc cgaaatctcg tctgccgcaa aacaccacct
     3361 taaccgaagt gtaattctct cttgccgggc atctgcccgg ctatttcccc tcagattctc
     3421 ctgatttgca taaccctgtt tcagccgtca tcataggctg ctgttgtata aaggagacgt
     3481 tatgcaggat ttaatatccc aggttgaaga tttagcgggt attgagatcg atcacaccac
     3541 ctcgatggtg atgattttcg gtattatttt tctgaccgcc gtcgtggtgc atattatttt
     3601 gcattgggtg gtactgcgga ccttcgaaaa acgtgccatc gccagttcac ggctttggtt
     3661 gcaaatcatt acccagaata aactcttcca ccgtttagct tttaccctgc aggggattat
     3721 cgtcaatatt caggcggtat tctggctgca aaaaggcacc gaagcggcag atattctgac
     3781 tacctgcgcg cagttgtgga tcatgatgta tgcgctgctt tcagtcttct cgttgctgga
     3841 tgttattttg aatctggcgc agaaattccc ggcggcatct cagttaccgc tgaaagggat
     3901 atttcagggg attaaactga tcggcgcaat cctggtcggc attttgatga tctcgctgct
     3961 gattggtcag tcgccagcga ttctaatcag cggtcttggt gcaatggctg ccgtgctgat
     4021 gttggtattt aaagatccga ttcttggtct ggtggcaggt attcagcttt ccgcgaacga
     4081 tatgctgaaa ctgggcgact ggctggaaat gccgaaatac ggcgcggatg gcgcggtgat
     4141 cgatattggg ttaaccaccg tcaaagtgcg taactgggac aataccatta ccactattcc
     4201 cacctggtct ctggtttctg actcctttaa aaactggagc gggatgtcag catctggcgg
     4261 gcgacgtatt aagcgcagta tcagtattga tgtcaccagt attcgttttc ttgatgaaga
     4321 cgaaatgcaa cgtctgaata aagcgcattt gttaaagcct tatttaacca gccgccatca
     4381 ggaaattaat gagtggaatc gccagcaagg ttctacggag tcggtattaa acctgcgccg
     4441 aatgaccaat attggaacct ttcgtgccta tctgaacgaa tatctgcgta accatccgcg
     4501 gattcgtaaa gatatgacct taatggtacg ccaactggct ccaggtgata acggtttacc
     4561 gctcgagatc tacgcgttta ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc
     4621 tgatatattc gatcacatat ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc
     4681 gccaaccggc aatgatattc gctctctggc gggtgcattt aagcagtaat taaaaaaacc
     4741 gctctcatcg aatggatgag agcggacatc gttttgtcgg atgtagcgtg aatgccttat
     4801 ttccgacgca gcgttttaaa tgccacaaac aggaatacaa tccacaccgg cagcaggatc
     4861 gctgacaagc gcatatcatc catcgtgcac atcagcagca aaatcatgcc gaggaaggcg
     4921 atgcaaagat agttgccgaa cggatagagc agcgccttaa actgtgtttc acgcccctga
     4981 cgtcgcatcg ccgcgcgaaa acgcagatgc gccaggcaga tcataatcca gttcaacagc
     5041 aacgttgcta ccaccagcgc catcagcaga ccaaacgctt tttgcggcag cagatagttg
     5101 attaacacca ccagcgaagt gatcgctccg gaaagcatca gcgagttaat cggcacaccg
     5161 cgacggctga cgcgagtcaa aaacttcggc gcattaccct gcacagaaag gccaaacagc
     5221 atgcggctgt tagagtaaac cccgctgtta tacaccgaca gcgatgccac tagaatgacg
     5281 aagttcagcg cagaagctac cacattgctg tcgagattat ggaaaatcat cacaaacggg
     5341 ctactgttgg atttcacttc cacccacgga tagagtgcca gtaaaaccac cagtgaaccg
     5401 atataaaaca gcaggatgcg atacaccacc tgatttaccg cttttggaat gcttttttcc
     5461 ggatcgcgcg cttcagcggc tgtaatccca atcagctcca gaccgccgaa ggagaacata
     5521 attaccgcca gcgacaaaat cagcccattc cagccggtgg cgaagaaacc accgtagcgc
     5581 cagaggttgt cgatactggc tttctcgccg ccgtaaccag aaaacagcag ccacaggcca
     5641 aagccgatca taccgatgat cgccagcact ttaatcagcg caaaccagaa ctcggtttcg
     5701 ccatataagc gcacgttcac caggttaacg gcgttgatga taataaagaa ggcggcagcc
     5761 caaatccacg ttggaacatc cgggaaccag tactgcatat agatgcccgc agcggtcagc
     5821 tctgccattc ccaccagcac gaacattacc cagtagttcc agccagagag gaagcccgca
     5881 aacggtcccc agtatttata ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc
     5941 accatctcgc caagctggcg cataatcagg aaagcgatga tcccggcgac gccgtagccc
     6001 agcaatacag ccggacccgc catctgaatc gccgggccaa tgccaagaaa cagaccagta
     6061 ccaattgcgc cacccagcgc aatcagttga atatgacggt tatgtaatcc gcgatgaagc
     6121 gtcggctctt gattcgacgc agtatcttcc gatacggttg acgcgttttt cacgcctttc
     6181 ccctgtgtgt cttttttgtt gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga
     6241 cacaatccac acggttaaac gaggtatcct gctttaattc cgtacccgat gtcggtgcag
     6301 ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggtg cggtgatcat
     6361 gccgccaatc atcggtgcgg caatccggct catcacctct gaaccagccc ccgttcccca
     6421 cagaatcggc agcagaccgg cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg
     6481 caggaccgcg ccgtgataca acgcctcatc cagcttctgc tcgctgaatg tttgtagatt
     6541 attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca ccacgccaaa
     6601 ttcggcggcg accccggcaa gggcaataaa gccggtaccc gtcgccacag aaagatgaaa
     6661 gcccatccac cacagtaacc agatgccgcc caccagcgca aacggtacgc tgctgataat
     6721 cagcaacgct tcgcccaccc gacggaatgc cagatacaac agcacgaaga tgatcatcag
     6781 tgtcatcggc accatgagtt tcagcttatg gttggcgcgc tccagtagct cgaactgccc
     6841 ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg ctttttgcaa
     6901 atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg
     6961 gcgcgcattc tcggttttca gcatcgacgg tccggtagag actttaacgt cggccacgtc
     7021 tgccagggtg atttgctgct tcatcggcgt caggatcggc aactggcgca gtgcctgcgg
     7081 actatcgcgc cagctttgcg gataacgcag attaattgga taacgggcaa tcccttccac
     7141 cgtttcgcca accatcgccc cgcccaccgc agaagtcaca aacaactgca catccgccac
     7201 cgtcataccg taacgtgcgg ctttttcacg gttaatctca acgttgatat agcgcccacc
     7261 ttccagccgc tcagcaagcg cagaagctac gcctggcacc gttcgcgcca cttcttcaat
     7321 ttgctcagcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa tgccgatggg
     7381 gcttttaatg ccggttgaga gcatatcgat acggttacga attggcggca cccacagatt
     7441 cgccagcccc ggcagacgca cggtgttatc cagttcctca atgattttgt ccattgtcat
     7501 gcctggccgc cactgatcct gtggcttaag ctggatggtc gtttctacca tctccagcgg
     7561 agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg ccacttcagg
     7621 tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg ctgcggaaat
     7681 ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc
     7741 cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgccg ccaccagcag
     7801 cgtggttttc ggccagtgca gtactttcag caacagcgga tgataaacac gaatcaaaaa
     7861 gcgattgagc ggattactgc tttccggcgg aattttgcca cggatccagt aacccatcag
     7921 gatagggatc actacgatcg ccagcagcac cgcacccgcc atcgcatacg ttttggtgaa
     7981 cgccaacggg ccaaacagac gcccttcctg cccttccagg gtgaagatcg ggataaacga
     8041 caacgtgata atcagcagac tgataaacag cgccggcccc acttcaacag acgcatcggt
     8101 gatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt gctgccactc
     8161 ttccagccgt ttatgcgcat tctcgatcat gacgatagcg gcatcgacca tcgccccgac
     8221 ggcaatcgca atgccaccca gcgacataat attggcattc agcccctgga agggcatgac
     8281 aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac
     8341 atgccagaga aacggcgcgc agactaccgc caccacaata aactcttcca gcaacttgcc
     8401 gctgaggttg tcgatggccc ggtcaatgag ctggctgcgg tcgtatgttg taactatctc
     8461 cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg cggcgatcac
     8521 ttctcgggcg tttttgccgg atcgcaggat caccaccccg cccgccactt cgccttcgcc
     8581 gtttagttcg gcaatgcccc ggcgcatctt cgggccaatc tggaccttcg caacatcgcg
     8641 cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa agtcgtcgag
     8701 cgtttgtaga tagccgctgg cgcgcaccat atattctgct tccgccagtt cgattgacga
     8761 accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcga gactgatgcc
     8821 atactgcgcc aggcgctgag gatcgataac cacctgatac tctttcacca caccgcctac
     8881 cgacgccact tccgcaacgt caggaatggt tttcagctca tatttgagaa accagtcctg
     8941 caatgagcgt aaatcggcaa gatcgtgctt accgctgcga tccaccagtg catattcata
     9001 gatccagcca acacccgtgg catctggtcc cagctcggcg ctgactcccg caggcagctt
     9061 accctgtacc tggttgaggt actccagcac ccgcgaacgc gcccagtacg gatcggtgcc
     9121 atcttcgaaa atgacataca cataagagtc gccaaactgc gagaaaccac gcacagtctt
     9181 cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat tttcaacgat
     9241 ttgcggtgcc tggccgggat agctggtttt aataatcacc tgcacatcgg agagatccgg
     9301 caacgcatcc actggcgtat taatgatggt ccaggtgccc cagatgctca gaaacaacgc
     9361 gcccatcagc accaaaaaac ggttcgccac cgagcgacga ataatccatt caatcattgg
     9421 ttattccctc aatgcgcatg ggtagcactt tcagagcgca tccgctccag tgcgccagaa
     9481 atattggctt cggaatcaat caggaacagg ccgccggaaa ccaccttttc accttccgcc
     9541 agaccagagc gtaatgcggt gacgccttgc gatgcctgga aaacagcaac gcgtttcggt
     9601 acaaagcgcc cgtcggcatc aacggtaatc acccgctgtt cgttgccggt atcaatcagc
     9661 gcctgtgacg gaatgagcag catcggttcg ctggcggtgt tgagttgcaa ccaggcgttc
     9721 atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg
     9781 gtcgcggcat ccacgccagg tagcagcgtc catttgcgga tggtgagtgt tttatccggt
     9841 cgcgccggaa cggtgagggt aaactgcgag gcatctttca ccagccaggc gatggactcc
     9901 gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttcgcgatta cgcgcaatcc
     9961 ccctgagaac cgccatgaac aagggtttca tgatttgtga ttttagtttg gtacgcaatt
    10021 tggtacacaa cactattttc acttcagcgg gtagtcatca aactcaccat aacgagcatc
    10081 gttgatgatg tatgtgatca ccacaaatat cacatctgga ctagttccgt cttcatgcgc
    10141 ag
//
LOCUS       DAJTOW010000127        10104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTOW010000127 DAJTOW010000000
VERSION     DAJTOW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.127"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            123..254
                     /locus_tag="OO244_003474"
     CDS             123..254
                     /locus_tag="OO244_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5665172.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO244_003475"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO244_003475"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5665173.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO244_003476"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO244_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO244_003477"
     CDS             2687..3739
                     /locus_tag="OO244_003477"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5665174.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO244_003478"
     CDS             3815..5248
                     /locus_tag="OO244_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5665175.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO244_003479"
     CDS             5431..7611
                     /locus_tag="OO244_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5665176.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO244_003480"
     CDS             complement(7752..9035)
                     /locus_tag="OO244_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5665177.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="OO244_003481"
     CDS             complement(9170..10051)
                     /locus_tag="OO244_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5665178.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2451 a         2358 c         2553 g         2742 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcgcc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag tcaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgtatagct tcag
//
LOCUS       DAJTOW010000128         9925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTOW010000128 DAJTOW010000000
VERSION     DAJTOW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.128"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..336
                     /locus_tag="OO244_003482"
     CDS             <1..336
                     /locus_tag="OO244_003482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665179.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            524..1639
                     /locus_tag="OO244_003483"
     CDS             524..1639
                     /locus_tag="OO244_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665180.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2318..2536
                     /locus_tag="OO244_003484"
     CDS             2318..2536
                     /locus_tag="OO244_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5665181.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /locus_tag="OO244_003485"
     CDS             2754..2996
                     /locus_tag="OO244_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665182.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO244_003486"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO244_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5665183.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO244_003487"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO244_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5665184.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO244_003488"
     CDS             complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO244_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5665185.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO244_003489"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO244_003489"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5665186.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="OO244_003490"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="OO244_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5665187.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2396 c         2541 g         2499 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggtgcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttc
//
LOCUS       DAJTOW010000129         9895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTOW010000129 DAJTOW010000000
VERSION     DAJTOW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.129"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO244_003491"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO244_003491"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5665188.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO244_003492"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO244_003492"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5665189.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO244_003493"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO244_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5665190.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFILLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="OO244_003494"
     CDS             complement(4692..6452)
                     /locus_tag="OO244_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5665191.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="OO244_003495"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="OO244_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="OO244_003496"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="OO244_003496"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5665192.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="OO244_003497"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="OO244_003497"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5665193.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2518 a         2363 c         2480 g         2534 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 ataaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgtcgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgt
//
LOCUS       DAJTOW010000130         9773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTOW010000130 DAJTOW010000000
VERSION     DAJTOW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.130"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO244_003498"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO244_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5665194.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO244_003499"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO244_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5665195.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO244_003500"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO244_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5665196.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO244_003501"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO244_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5665197.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO244_003502"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO244_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5665198.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO244_003503"
     CDS             complement(6031..6360)
                     /locus_tag="OO244_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5665199.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO244_003504"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO244_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5665200.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO244_003505"
     CDS             7998..8171
                     /locus_tag="OO244_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5665201.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO244_003506"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO244_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5665202.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2562 a         2286 c         2434 g         2491 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcgcc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atc
//
LOCUS       DAJTOW010000131         9683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTOW010000131 DAJTOW010000000
VERSION     DAJTOW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.131"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(26..214)
                     /locus_tag="OO244_003507"
     CDS             complement(26..214)
                     /locus_tag="OO244_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5665203.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO244_003508"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO244_003508"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5665204.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO244_003509"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO244_003509"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5665205.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO244_003510"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO244_003510"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5665206.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO244_003511"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO244_003511"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5665207.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO244_003512"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO244_003512"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5665208.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO244_003513"
     CDS             5109..6242
                     /locus_tag="OO244_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5665209.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO244_003514"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO244_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5665210.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO244_003515"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO244_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5665211.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO244_003516"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO244_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5665212.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO244_003517"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO244_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5665213.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO244_003518"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO244_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5665214.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="OO244_003519"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="OO244_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5665215.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASRFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2466 a         2480 c         2466 g         2271 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccagat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tct
//
LOCUS       DAJTOW010000132         9596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTOW010000132 DAJTOW010000000
VERSION     DAJTOW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.132"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO244_003520"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO244_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5665216.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO244_003521"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO244_003521"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5665217.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO244_003522"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO244_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5665218.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="OO244_003523"
     CDS             complement(2404..2607)
                     /locus_tag="OO244_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665219.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="OO244_003524"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="OO244_003524"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5665220.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGSKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="OO244_003525"
     CDS             complement(3803..4015)
                     /locus_tag="OO244_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5665221.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="OO244_003526"
     CDS             4275..4583
                     /locus_tag="OO244_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5665222.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO244_003527"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO244_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5665223.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO244_003528"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO244_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5665224.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="OO244_003529"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="OO244_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5665225.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="OO244_003530"
     CDS             complement(8479..8982)
                     /locus_tag="OO244_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5665226.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9596)
                     /locus_tag="OO244_003531"
     CDS             complement(9070..>9596)
                     /locus_tag="OO244_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5665227.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2451 c         2285 g         2478 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attaccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggagcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagc
//
LOCUS       DAJTOW010000133         9512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTOW010000133 DAJTOW010000000
VERSION     DAJTOW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.133"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..593)
                     /locus_tag="OO244_003532"
     CDS             complement(<1..593)
                     /locus_tag="OO244_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5665228.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="OO244_003533"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="OO244_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5665229.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="OO244_003534"
     CDS             complement(1422..1961)
                     /locus_tag="OO244_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5665230.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="OO244_003535"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="OO244_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5665231.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="OO244_003536"
     CDS             complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="OO244_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5665232.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="OO244_003537"
                     /pseudo
     CDS             complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="OO244_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6185..6901
                     /gene="nanC"
                     /locus_tag="OO244_003538"
     CDS             6185..6901
                     /gene="nanC"
                     /locus_tag="OO244_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5665233.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6921..8027
                     /gene="nanM"
                     /locus_tag="OO244_003539"
     CDS             6921..8027
                     /gene="nanM"
                     /locus_tag="OO244_003539"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5665234.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8092..9072
                     /locus_tag="OO244_003540"
     CDS             8092..9072
                     /locus_tag="OO244_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5665235.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2786 a         2074 c         1992 g         2660 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg
     3001 gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac
     3061 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
     3121 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
     3181 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
     3241 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
     3301 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
     3361 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
     3421 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
     3481 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
     3541 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
     3601 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
     3661 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
     3721 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta
     3781 aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt
     3841 caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag
     3901 tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt
     3961 tgaagaacat aaatcattag attttgtatg ggtatggtta acttaatgtt tattatgata
     4021 attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt ttgatatatc
     4081 gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc
     4141 atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc
     4201 ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt
     4261 atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat
     4321 ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg
     4381 agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga
     4441 agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt
     4501 tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc
     4561 cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat
     4621 cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat
     4681 ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc
     4741 ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct
     4801 gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct
     4861 gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat
     4921 gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc
     4981 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     5041 aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga
     5101 cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt
     5161 agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag
     5221 cttaggccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga
     5281 cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc
     5341 tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     5401 aacactaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca
     5461 aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta
     5521 ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat
     5581 tatttataaa acatatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt
     5641 cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa
     5701 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     5761 agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc
     5821 agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga
     5881 tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta
     5941 cgaatattca aatcaattaa aaatcagaag agattttata ctcaggtatt taatctggat
     6001 ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata
     6061 taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac
     6121 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
     6181 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
     6241 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
     6301 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
     6361 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
     6421 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
     6481 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
     6541 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
     6601 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     6661 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga
     6721 ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca
     6781 tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc agggtgttta
     6841 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     6901 gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt
     6961 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     7021 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca
     7081 agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac
     7141 cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg
     7201 gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     7261 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt
     7321 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     7381 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc
     7441 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     7501 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     7561 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     7621 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     7681 atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     7741 ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     7801 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     7861 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     7921 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     7981 cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     8041 cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca
     8101 ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     8161 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa
     8221 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt
     8281 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     8341 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
     8401 ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg
     8461 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     8521 tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca
     8581 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     8641 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     8701 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
     8761 tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat
     8821 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     8881 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     8941 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     9001 acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg
     9061 cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga
     9121 caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc
     9181 aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt
     9241 attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg
     9301 gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac
     9361 cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat
     9421 gatatgctta atgatgttca aagaaattag gccattatta tattattgct tgcattttct
     9481 ttcattccta aaagcacccg ccattaatcc ca
//
LOCUS       DAJTOW010000134         9493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTOW010000134 DAJTOW010000000
VERSION     DAJTOW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.134"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..190)
                     /locus_tag="OO244_003541"
     CDS             complement(<1..190)
                     /locus_tag="OO244_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5665236.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO244_003542"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO244_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5665237.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO244_003543"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO244_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5665238.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO244_003544"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO244_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5665239.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO244_003545"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO244_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO244_003546"
     CDS             complement(3262..3381)
                     /locus_tag="OO244_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5665240.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO244_003547"
     CDS             complement(3342..3527)
                     /locus_tag="OO244_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665241.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO244_003548"
     CDS             3628..3801
                     /locus_tag="OO244_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665242.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO244_003549"
     CDS             3803..4147
                     /locus_tag="OO244_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5665243.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO244_003550"
     CDS             4151..4486
                     /locus_tag="OO244_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5665244.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO244_003551"
     CDS             complement(4543..6864)
                     /locus_tag="OO244_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5665245.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO244_003552"
     CDS             complement(6981..7190)
                     /locus_tag="OO244_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665246.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO244_003553"
     CDS             complement(7941..9464)
                     /locus_tag="OO244_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5665247.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2658 a         2205 c         2081 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAJTOW010000135         9275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTOW010000135 DAJTOW010000000
VERSION     DAJTOW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.135"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            2..853
                     /gene="hchA"
                     /locus_tag="OO244_003554"
                     /pseudo
     CDS             2..853
                     /gene="hchA"
                     /locus_tag="OO244_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
     gene            complement(961..2319)
                     /gene="hprS"
                     /locus_tag="OO244_003555"
     CDS             complement(961..2319)
                     /gene="hprS"
                     /locus_tag="OO244_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5665248.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2319..2990)
                     /gene="hprR"
                     /locus_tag="OO244_003556"
     CDS             complement(2319..2990)
                     /gene="hprR"
                     /locus_tag="OO244_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5665249.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3123..3536
                     /gene="hiuH"
                     /locus_tag="OO244_003557"
     CDS             3123..3536
                     /gene="hiuH"
                     /locus_tag="OO244_003557"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5665250.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3645..4649
                     /gene="msrP"
                     /locus_tag="OO244_003558"
                     /pseudo
     CDS             3645..4649
                     /gene="msrP"
                     /locus_tag="OO244_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4650..5285
                     /gene="msrQ"
                     /locus_tag="OO244_003559"
     CDS             4650..5285
                     /gene="msrQ"
                     /locus_tag="OO244_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5665251.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5543..6193
                     /gene="zinT"
                     /locus_tag="OO244_003560"
     CDS             5543..6193
                     /gene="zinT"
                     /locus_tag="OO244_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5665252.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7271..7540)
                     /locus_tag="OO244_003561"
     CDS             complement(7271..7540)
                     /locus_tag="OO244_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5665253.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7597..8265
                     /locus_tag="OO244_003562"
     CDS             7597..8265
                     /locus_tag="OO244_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5665254.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8581..>9275
                     /locus_tag="OO244_003563"
     CDS             8581..>9275
                     /locus_tag="OO244_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5665255.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2658 a         2088 c         1897 g         2632 t
ORIGIN      
        1 tatgactgtt caaacaagta aaaacccgca ggtcgatatt gctgaagata atgcgttctt
       61 cccttcagaa tattcgctta gccaatatac gagtcccgtc tctgatcttg atggcgtgga
      121 ctatccaaaa ccgtatcgcg gtaaacataa aattctggtg atcgccgcgg acgaacgtta
      181 tttgccgacc gataacggaa aactgttctc gaccggtaac catccgattg aaacgttgct
      241 gccgttgtat catctccatg ctgcaggttt cgaattcgaa gtggcgacca tttccggtct
      301 gatgaccaag tttgaatact gggctatgcc gcacaaagat gaaaaagtaa tgccattctt
      361 tgaacagcat aaatcgttat tccgtaatcc gaagaaactc gcggatgttg tcgccagcct
      421 caacgctgat agcgaatatg cagcaatctt tgttcctggt gggcatggcg cacttattgg
      481 tttaccagaa agctaggacg tggctgccgc tttacagtgg gcaatcaaaa atgaccgttt
      541 tgttatctcc ctttgccacg gtccggcggc ttttctggca cttcgccaca gcgataaccc
      601 actgaatggt tattctattt gcgcattccc agacgccgca gacaaacaaa cgccagatat
      661 tggctatatg ccgggtcatc tcacctggta cttcggcgaa gagttgaaga aaatgggcat
      721 gaacatcatt aatgacgaca tcaccgggcg agtacataag gaccgtaaac gtctcaccgg
      781 cgacagtcct tttgcagcga atgcgttggg taaactggcg gcgcaggaaa tgctggcagc
      841 ttacgcgagt taatccctgt tacattgtga tgtaagcgca taatcaatcg cagccacgtt
      901 aatactcacg acgtggctgc gttttttcac ggttaactta tggcgtactg aagcccgatg
      961 ttaatttctt tgcggtaacg taattcggaa cacgttatgc ttactgagat agtgatacgt
     1021 agcacttcct ccatgtaatt cggcaatcgc tttgactaaa gaaaggccaa gtccctgacc
     1081 tacggaatga cgcgaattat ctccccgcca aaatctacgg aagagttttt caggctcatt
     1141 aattttcgtt ccaggactgg cgatatcaat attaagagag ccgttggcat caagaaaact
     1201 ggttatatga atacgcgatt tttctggtga atatcgaatg gcgttaacaa taagattcga
     1261 taacattcgt tgcagtaaaa ttttatccgc aaagatttgt tgattgcact tgaccttaaa
     1321 gcaaatctct ttctcgtctg aaagatattc aagatagtcc aaaagatttt caacttcctt
     1381 attgagagaa agcgaatcca gtttcaccaa aacattgttt ttgtctgccc tggcaagaaa
     1441 cagtatattc tctgttaacc gcgaaatatt ttccagctct tcaatgtttc cggcaattgt
     1501 tttttgatat tcagcgatac ttctgatttg actgagcgta acctgattct gacccagtaa
     1561 tgcattaatt ggcgttctaa gttcatgagc gagatcgtca gcaaattgac taaggcgctc
     1621 aaaatctttg actaaagcat gatgcatttt attcaacgcc tgcccaagag gttttagttc
     1681 tctcggtaat gcgttaacct caaccggcac tcggctatcg ttataattca gcgcttccgt
     1741 tacgccactc aactttttga tctctcgcaa ccccgtcctg attaacaacg gacttaatac
     1801 tgagcacagt ataatggcga caatgcaaat tataatgcta ttaattttat actgttcaag
     1861 catattatgt ctggctgaag ccaatttagc cacagtaacc gttaatgatg gcgaaacttc
     1921 atcaatgtta attcgtaaag catctatctc tgtatcatta ataatgcttc tgtaaatgcc
     1981 tgctgcgctg attgtctcac tagcaggtat gttatttaac atgtcatcac tgacatttgt
     2041 ccggttaaca attttattga tgccatcact atgaatgatc aaaatatcct gactaacatc
     2101 catcatccgg ttaaagtaca caggtaacgt atctggattt accccatcaa ttaacaactg
     2161 cttgatctgc gctgtccggt taatgagtgt tgtatcatcg cgccatttca actcacttgc
     2221 caggccatta tagagagtcc atacaattcc agcgccagca acagactgca gaaatataaa
     2281 aagcaaggtt aaacggacgg ttatagataa tcttttcatt atttttttac cgctacgaat
     2341 gaatagccca tcccccggat tgtggcgatt agcttttcag gaaaaggatc atcaactttt
     2401 gcgcggagcc tgcgaatggc gacatccacc gtattggtat cactatcaaa gttgattccc
     2461 caaatttcac tcgcaataac cgttctgggt ataatctcgc cagctctgga ggccagtagc
     2521 caaagtaact gaaactcctt gcgcgtcagt gtaatactga tattgtccct gctgacactt
     2581 tgactgacag agtccatttt taagccactg atttctaatg ttgaattcag agcgtgatgt
     2641 tgccttaatt gtgcccgaac ccttgctagc aactcagaaa atgaaaaagg ttttaccaga
     2701 taatcatttg ccccactgtc caaccctctg actctgtcat cgacagaatc ccttgcagta
     2761 aggcaaataa caggggtttg ctttgccgtt cttaacgttt gtaagatctg ccagccatcc
     2821 atacccggaa gcataatatc cagaatgatc aatgcataat catccttcag cgcaagataa
     2881 agcccatctc tgccatcaga aacggcatca atgacataac ccgcttcgga aagcccctgc
     2941 gttacccatt cctgggtcct ttgattatct tcaataagta gaatcttcat gaaaataata
     3001 tgccataaat catatgttat agaaacaacc tggttcatta caaaattgta atgctgctgt
     3061 aaggttaccc tggacgtttt ttcgctatcc tcaaaactca ttcacatgac aaggatataa
     3121 acatgttaaa gcgttattta gtactctcgg tagcaacggc agccttttca ttaccttctt
     3181 tggtttatgc cgcacaacaa aacattctta gcgtgcacat tttgaaccag caaacaggaa
     3241 aacccgctgc cgacgtgaca gtcactcttg aaaagaaagc ggataacggc tggttacaac
     3301 ttaataccgc caaaacagat aaggatggac gaattaaggc actgtggccc gagcaaactg
     3361 cgactactgg cgattaccgt gtcgtattta aaaccgggga ctatttcaag aaacaaaatc
     3421 ttgaaagttt cttccctgag attcccgttg aatttcatat taataaagtg aacgagcatt
     3481 atcatgtgcc tttgttactc agccaatatg ggtattcaac ctatcgtggc agttaatgcg
     3541 taaatatgta aaagctgatc tctgctactg ttagggaggt gactccctga cagattaaca
     3601 gtaaacagct cttgttggtt aacgacaaaa aagtgtgatg gcttatgaaa aagaagcaat
     3661 ttttaaaaga atcagatgtt acggccgagt cggtattctt tatgaagcgt cggcaggtgt
     3721 taaaagcact gggcatcagc gcagctgcat tttctttgcc tcacgctgcg catgccgatc
     3781 tgcttagctg gtttaaaggg aacgatcgtc cgcccgcccc cgccggaaaa ccgctggagt
     3841 tcagcaagcc tgccgcctag caaaataacc tgccactgac gccagcagat aaagtctccg
     3901 gttataacaa cttctatgaa ttcgggctgg ataaagacga tcccgcggct aatgctggta
     3961 gcctgaaaac cgatccatgg acactgaaaa tcagcggcga agtggcaaaa ccattgaccc
     4021 tcgatcatga tgatttaacc cgtcgcttcc cgctggaaga gcgtatttat cgtatgcgct
     4081 gcgtggaagc gtggtcgatg gtggtgccgt ggattggttt tccgctacac aaattgctgg
     4141 cgcttgccga acccaccagc aatgcgaagt atgtcgcttt cgaaacgatt tatgcgccgg
     4201 aacagatgcc tggccagcag gaccgcttta tcggcggcgg gctgaaatat ccttatgtcg
     4261 aaggattgcg tctcgacgaa gcaatgcatc cgctcacact gatgaccgta ggtgtttatg
     4321 gcaaggcgtt accaccgcaa aatggcgcgc cggtgcgact gattgtgccg tggaaatatg
     4381 gctttaaagg gattaaatcg atcgtcagta ttaagctgac ccgcgagcgt ccgccaacca
     4441 cctggaatct ggcagcgcct gacgaatacg gtttttacgc caacgttaat ccgcatgttg
     4501 atcacccgcg ctggtcacag gctaccgaac gatttattgg ttcaggcggc attctcgatg
     4561 ttcagcgcca gccaacacta ctgtttaatg gttacgccga ccaggtggca tcgctgtatc
     4621 gtggcctgga tttgcgggag aatttctaaa tgcgtctgac agcaaaacaa gtgatatggc
     4681 tgaaagtttg cctgcatctt gccggattgt tgccgtttct ctggctggtc tgggcgatca
     4741 atcacggtgg actgggtgcc gatccggtga aagatattca gcattttacc ggtcgcactg
     4801 cactgaaatt tttgcttgcg gccttgttaa tcacacctct ggcacgctac gcaaaacagc
     4861 cgttattgat acgcactcgc cgcctgttag gcttatggtg ctttgcctgg gcgacactgc
     4921 atttaaccag ttacgcattg ctggagttgg gcgtgaataa tctggcgtta ttaggtaagg
     4981 agttaattac ccggccttat ttaacgttag gaattatcag ctgggttatt ttgcttgctt
     5041 tagcgttcac ttcaacccag tcgatgcagc gaaaactcgg caaacactgg caacagttgc
     5101 ataacttcgt ctatcttgtc gcgatcctgg ccccaataca ttatctgtgg tcagtgaaga
     5161 ttatttcacc gcagccactc atctacgccg ggctggctgt actgctttta gccttacggt
     5221 ataagaagtt gctttccctt ttcaaccggt tacgcaagca ggcccacaac aaattatctc
     5281 tataacttca tattgccgac aaacttcgcg ttatcccggc cggatgcggc gcgagcgccc
     5341 tatccggccc acacagatcg catcaagtta tgtttcgttt gtcatcaacc tttcatcacc
     5401 agaataattt attttatttc aataagatag ttcaatacac ctgagaatac catgctctca
     5461 tttcctaatg agttgttgca ttttttcata tgttacaata taacattaca cataatgtgc
     5521 attaactctg gaggaaattg ttttggcgat tcgtcttcat aaactggctg ttgctttagg
     5581 tgtctttatt gttagcgctc ctgccttttc gcatggtcat cattctcatg gcaaaccctt
     5641 aacagaggtc gaacaaaaag ctgctaatgg tgtttttgat gataccaatg tacaaaaccg
     5701 aacgctcagt gactgggatg gagtctggca atccgtttac cctttactgc aaagtggcaa
     5761 acttgatccc gtctttcaga agaaagcgga tgcagataaa accaaaacat ttgctgaaat
     5821 taaagattat tatcgcaaag gttatccgac agatatcgaa atgattggca ttgaggacgg
     5881 cattgtcgaa ttccatagaa ataatgaaac aacatcctgt aaatatgatt acgatggata
     5941 caaaatactc acctataaat caggcaagaa aggcgttcgt tacttattcg aatgtaaaga
     6001 tcctgaaagc aaagccccta aatatataca atttagcgat catataattg caccacgaaa
     6061 atcatctcat tttcacattt ttatgggtaa tgattcccag caatctttat tgaatgaaat
     6121 ggaaaactgg ccaacgtatt atccctatca gttgagtagc gaagaagtgg tcgaggagat
     6181 gatgtctcat tgattttttt gcgctgactt cagttattta acagaacagt gcctggtagt
     6241 aacaacaata atttaccaag acaaataagt aagacaaact cagacacgct catacttgag
     6301 tcgtgtcttt tttattatgt ctgtagaaaa aaactgagaa aaaggctaac aataaataca
     6361 caactcagcc atacagactg gcagcgcaaa tgtttccagg agcaagtcat ctgtcatggt
     6421 aaagctaacc agaaatatag gttaattttg gtgtttaaac cttttttact tgttagtatg
     6481 atacaaaggc tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt
     6541 cccgcggctt taatctgacg gccgcgttcc ttttttggct caattattga ccccttctct
     6601 acgcaacttc agttcccgcc agcaactttg cggcagtttc ataggatcaa tgtctaaaag
     6661 aataatggtg accgataaga aaacgactga ataactgtag attttcgctc gaaaccttcc
     6721 tgtcagctcc atagcgaatc aagcgctgaa tgtcacagta tcgaacagaa aacagtgacg
     6781 atctaaccct tcaagaatat tctacgattg ttctgtttag gaaaagcaag gcgggaagtc
     6841 gggagataag tcattgataa aatggcggag agagggggat ttgaaccccc ggtagagttg
     6901 cccctactcc ggttttcgag accgatgata cagagtcgaa aaatcaaaga gttaaaaatt
     6961 tttcttggaa aaaatgtctt aaaatcgtac acgaaaaatc aatgaattac ataacattaa
     7021 agaagaatat tccaagcctt cttgagtcca ccacctgttt tcaggtgtca ttcagacgtg
     7081 atttgcccca gattgggaaa gcacgctttt ttctcagctt attccgtttt gcctgtgtgt
     7141 tgaataattc cactgcagtt atataaaatc ggtaacggct ggaaatcatt caatactcgc
     7201 actatcgaaa gtttaccagc caaccgcagc acgtcctgca tacgtcgtgt ctgcggtttt
     7261 tctttttcgc ttacattgtg tctggttctt ccggccactc aatatcaggt gcagttgatg
     7321 tatcaacacg gttcagcaac acccgatact tcttccaggc ttccagcaac gagttttctt
     7381 cctccgttgc gatctccaga tctacagcat cctgaagtgg cgcaatatgc tcactgaatc
     7441 cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc tgtatcgaag
     7501 cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt gtaattcatc
     7561 gccataattg atttaattca caaataaaac tataatatgg tgaaattaat gaaaaaaaac
     7621 acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt
     7681 ttgggagtat caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac
     7741 tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg gttttacctt
     7801 actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac
     7861 gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca tgatgttttt
     7921 gaaccttatc ctgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt
     7981 cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc
     8041 ttaactgatg atggaactct atacggagcc acaattcttg gcgatggagt tgtgcacaat
     8101 agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa
     8161 gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag
     8221 gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc aaccgcagca
     8281 cgttcttgct taagacgtgc tacggtttcc tttacgattt ggggttgcga ctttacctct
     8341 attgataatg cattccggca gaacgttcaa atatgaacgt acgatattta actaaccgaa
     8401 aaacaaaata tagcacaggc gagacattta ccatctaaga aaatagtatc gtttttttac
     8461 agttaaatca acatcacttc cttaaaatga aaacaataaa tttcaatcct gaaacatttt
     8521 atttcagctt attattcccc aggatcacat tttatcagga tatccttctg cgaggttatt
     8581 atgcttcctg taaataatcc ccccctatcc actggaaacg tctcttttta cagaactaca
     8641 tcaatcgaca atgttcacaa taattatctc tccgaatggg ttgaatggac taaaaacagc
     8701 atttccggag aaaacaggga aactgctttt acccggctcc aattatgtct ggagaacagt
     8761 gaaacatcgt tggacttatc ttgtttaggt ctcagatctc taccacgatt gcctgacaat
     8821 cttgatgaaa ttaatgtaag caataaccaa ctatcaatgc tccccgagct accaagggca
     8881 ttgaaagagc tgaatgcaag cagtaatcaa ttatctgcac ttcctgaatt accagtgtcg
     8941 ctggaatata taaatgtgag tgataaccat ttgttcgcac ttcctgaatt acctgcgtca
     9001 ctagaatata ttaatgtaag tgacaatcac ctgtctgtac ttccgaggtt accaatgtca
     9061 ttggaattac ttgatgcagc cagaaatgct ttggaagtaa taccagattt tccagaaaga
     9121 gatgatcata ttataagaat attctggctt aatcagaacc ggatcacggc aattccggaa
     9181 agcatacttg gcctcagttc tgatagcgtt gtcaatctta gagaaaatca actatctccc
     9241 agaataatgc aaactttgtt acaacaaacc gccca
//
LOCUS       DAJTOW010000136         9254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTOW010000136 DAJTOW010000000
VERSION     DAJTOW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.136"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO244_003564"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO244_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5665256.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO244_003565"
     CDS             1828..3147
                     /locus_tag="OO244_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5665257.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO244_003566"
     CDS             3445..3486
                     /locus_tag="OO244_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665258.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO244_003567"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO244_003567"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5665259.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO244_003568"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO244_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO244_003569"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO244_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO244_003570"
     CDS             5815..6711
                     /locus_tag="OO244_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5665260.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSATLMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OO244_003571"
     CDS             6708..7694
                     /locus_tag="OO244_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5665261.1"
                     /translation="MTQPVLDIQQLHLNFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCTCWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OO244_003572"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OO244_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO244_003573"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO244_003573"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5665262.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2264 a         2262 c         2319 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ctttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgaatt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcacctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggcaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgacc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caag
//
LOCUS       DAJTOW010000137         9164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTOW010000137 DAJTOW010000000
VERSION     DAJTOW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.137"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="OO244_003574"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="OO244_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5665263.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="OO244_003575"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="OO244_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5665264.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO244_003576"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO244_003576"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5665265.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO244_003577"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO244_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5665266.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="OO244_003578"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="OO244_003578"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5665267.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO244_003579"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO244_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5665268.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..>9164
                     /gene="entF"
                     /locus_tag="OO244_003580"
     CDS             5545..>9164
                     /gene="entF"
                     /locus_tag="OO244_003580"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5665269.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YA"
BASE COUNT         1992 a         2423 c         2531 g         2218 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggc
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggatgcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggcattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctga
//
LOCUS       DAJTOW010000138         9147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTOW010000138 DAJTOW010000000
VERSION     DAJTOW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.138"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OO244_003581"
     CDS             complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OO244_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5665270.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OO244_003582"
     CDS             complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OO244_003582"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5665271.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OO244_003583"
     CDS             complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OO244_003583"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5665272.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3793..4302)
                     /gene="def"
                     /locus_tag="OO244_003584"
     CDS             complement(3793..4302)
                     /gene="def"
                     /locus_tag="OO244_003584"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5665273.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4450..5574
                     /gene="dprA"
                     /locus_tag="OO244_003585"
     CDS             4450..5574
                     /gene="dprA"
                     /locus_tag="OO244_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5665274.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5546..6019
                     /locus_tag="OO244_003586"
     CDS             5546..6019
                     /locus_tag="OO244_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5665275.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6048..6590
                     /locus_tag="OO244_003587"
     CDS             6048..6590
                     /locus_tag="OO244_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5665276.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6595..7167
                     /gene="tsaC"
                     /locus_tag="OO244_003588"
     CDS             6595..7167
                     /gene="tsaC"
                     /locus_tag="OO244_003588"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5665277.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7172..7990
                     /gene="aroE"
                     /locus_tag="OO244_003589"
     CDS             7172..7990
                     /gene="aroE"
                     /locus_tag="OO244_003589"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5665278.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7987..8244
                     /locus_tag="OO244_003590"
     CDS             7987..8244
                     /locus_tag="OO244_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5665279.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8220..8774)
                     /locus_tag="OO244_003591"
     CDS             complement(8220..8774)
                     /locus_tag="OO244_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5665280.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2298 a         2218 c         2279 g         2352 t
ORIGIN      
        1 tctaacaaat gttaagccat tttcctgcca ggaatgaatt taattaatcc ttgataatca
       61 aagggaaata ataaggcgtc attagacgcc ttattaatta caagaagaaa gggcttggct
      121 ggaagagtct ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat
      181 cgccttgctc aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg
      241 ctccaataat cgttcctggc ggtagcttga tttcgtcaat gactctgccg acaacgcgtg
      301 aggtgctttc atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcaatg
      361 aggaaacacc aacaatatct gcttttcgca catggctaag caacgcagaa atagttgctt
      421 gttgtggtga aattgcaata tcgatcacgc tcccctgaac cagatccaca taagcgcgac
      481 gctggatcaa taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga
      541 tattggcctc gtcatcgttg gtgacggcaa taaacagatc aacttgatcg atatgttctt
      601 cggccagcag ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt
      661 ccgccagttc ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt
      721 ccagacgacg cgccagccct gcaccgatat taccgccacc aaccagcatg atccgtttat
      781 acggtttttc cagtcgctgt aattcactca tcaccgcgcg gatatgctgt gaagcggcaa
      841 taaagaacac ttcatcacca gcttcaacaa tggtcgaacc ttgtggacga atggggcgat
      901 cgtggcggaa aattgctgcc acacgagtat cgatatgtgg catatgttcg cgcatggtcg
      961 aaagtgcatt accaatcagc gggccgccat aataggcttt aactacagcg aggctgactt
     1021 taccctcagc gaagttcacc acctgcaatg cgcctggata ctcaatcagt cggtaaatat
     1081 tatcgataac caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa
     1141 atagcttatc ggcatcgcgt acgtagtctg gtgagcggat gcgagcgatg cgattagggg
     1201 tgttgaaaag tgagtaggct acctggcagg caaccatatt ggtttcatct gaactggtta
     1261 cagcaaccag catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc
     1321 catgcccctg cacgactcgc aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt
     1381 tggtatcgac aacagtaata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc
     1441 caacctggcc ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact
     1501 ttttgattag cttagcgtaa aagaagccgt cgccctcttc ggcaccaggc tgattttgtt
     1561 tacccggttg ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa
     1621 aggctttaat ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg
     1681 ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga
     1741 gttccgggat atcgcgatcg cgacgtaacc atttaatatc tggatggcga cgaatcaccc
     1801 ccgttgctga acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt
     1861 gggaagggta acggccatca ccttgtttca cggttgcctt cataccaagg cgttttaaat
     1921 tgtcgtaaat gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg
     1981 gtgccacctc aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccaagatgt
     2041 gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg tgctgatgca tcctgaacgg
     2101 taacccatcc gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagccgta
     2161 cagcatcagg gtaatccgca tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa
     2221 gccagctgtc gcgggaatga tgcgtacgat taatacgcag ccacattggc ggacgctggt
     2281 tattggcttc ggcgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca
     2341 accaggaagg atgcagataa cgtgcatcac gggtattaaa ctcggctaat aactcttctt
     2401 gccgacgctg gaactggcgt aatacaccgt taatcaaccc tttaagttgc ggacgcttaa
     2461 ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg aggtggaatg cgggtataaa
     2521 gcagttgata caaaccaacc ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg
     2581 gacgggccat taacttatta atcagccagt ctaactgcga aagcgtacgc agtacgccaa
     2641 agcacaactc ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca
     2701 gaatgttgct taatgattgc ccttgctcga cgacttgttc aacggcctgg gccgccatgc
     2761 tacgtaaatt acgttgtttt ttcataagta taaaaatgct cggcaagacc gggcttagaa
     2821 gagtggacta tcagaccaga cggttgcccg gaacaaacca ttcccgacga gagttcagga
     2881 ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc
     2941 catcaccagt cgcaacctga atgccttgtt tgttggcttc aaggatcgtt cctggtgcag
     3001 cgttggttgc cgtatcaatg accgacgctt tccagacttt aaccggctgt ccttcaattt
     3061 ccagccagct cattggccat gggttgaaag cgcgaatgca gcgttcaagc tgtgctgccg
     3121 aaagtgacca gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag
     3181 tttcgtcctg aacttctggt ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa
     3241 gcccttgtgg gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag
     3301 taatcgggca ggagagctta tagagcatgt caccggtgtc taaaccaaca tccatttgca
     3361 taatggttac accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac
     3421 cgcgccagcg tggcagcagt gaaccatgaa tgttgataca gccaagacgc ggcatctcca
     3481 gcacagcttt aggcagaatt aaaccatagg cgacgacgac cataacatca gcctgcaaat
     3541 cggcgaccag ttgctggttt tcttgtggac gcagggaaac aggttgaaaa acgggcagac
     3601 cttcttcctc agccagaact ttaatcgggc tgggcatcag ttttttaccg cgtcctgccg
     3661 gtcggtctgg ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga
     3721 gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt
     3781 tagttcttat ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat
     3841 tcgttgttgt ttcagcggtg acagataatc cataaacagt ttgccaacca ggtgatccat
     3901 ctcatgctga atacagatag ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc
     3961 gcggtcaagg gcgcgaattt taactttctc tgcgcgcggc actaaagcac gttgttcagg
     4021 gatcgacagg caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt
     4081 gattaacacc agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg
     4141 gatatcaacc tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat
     4201 atcatcgacg atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg
     4261 aagccgctcg tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt
     4321 tcaggagtta gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat
     4381 cacttgattg acagcatcac tgaccaatcg caaagattgc taaggcttgc ttatggcagg
     4441 gagataagga tggtcgatat agaaatttgg ctgcgtttaa tgagcatcag cagcttgtac
     4501 ggcgatgata tggtccgtat cgctcactgg ctggcaaaac agtcgcatat tgatgcggtt
     4561 gtattgcagc aaacagggct tacattgcgg caggcacaac gctttctttc atttccacga
     4621 aagagtatcg aaagctcact ttgttggttg gagcaaccca accatcattt aatccctgcg
     4681 gacagcgaat tttatcctcc tcaacttcag gcgacgacag attaccccgg cgcactgttt
     4741 gttgaaggag aactgcacgc gctgcattca tttcagcttg ccgtagtggg gagtcgggcg
     4801 cattcatggt atggcgagcg atgggggcga ttattttgcg aaactctggc gacgtgtgga
     4861 gtgacaatta cgagtggact ggcgcgtgga atcgatggtg tggtgcataa agcggcctta
     4921 caggtaaatg gcgtcagcat tgctgtattg gggaatggac ttaataccat tcatccgcgt
     4981 cgccatgccc gactggctgc cagtctgttt gaacagggtg gcgctctcgt ctcggaattt
     5041 cccctcgatg ttccacccct tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt
     5101 ctaagtaaag gtgtactggt ggtggaagcg gctttgcgca gtggttcgct ggtgacagca
     5161 cgttgtgcgc ttgagcaggg gcgtgaagtt tttgccttgc caggtccaat agggaatccg
     5221 ggaagcgaag ggcctcactg gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa
     5281 gaaattctgg aaaacttgca atttggattg cactggttgc cagacgcccc tgaaaattca
     5341 ttttattcac cagatcagca agacgtggca ttgccatttc ctgagctcct ggctaacgta
     5401 ggagatgagg taacacctgt tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag
     5461 gtagttactc aactactcga actggagtta gcaggatgga tcgcagctgt acccggcggc
     5521 tatgtccgat tgaggagggc atgccatgtt cgacgtacta atgtatttgt ttgaaaccta
     5581 tattcacact gaagctgagt tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga
     5641 cgcaggtttt gagcgagaag atatctacaa tgccctgcta tggctggaaa aacttgctga
     5701 ttatcaggaa gggttggcag aaccgatgca actggcctcg gatcctctct ccatgcgtat
     5761 ttatacaccg gaagagtgtg aacgactgga tgccagctgc cgtgggtttc tgcttttcct
     5821 tgagcagatt caggtgctca accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc
     5881 gctggataac gcagagttcg aactggatga tctgaaatgg gtgatcctga tggtgttgtt
     5941 caacatcccg ggctgcgaaa atgcgtacca gcaaatggaa gaattactct ttgaagtgaa
     6001 tgaaggtatg ctgcattaat catttcttta attcagcata agttgttatg gcaaaatcag
     6061 cactgttcac ggtgcgtaat aatgagtcct gcccaaagtg cggggctgaa ctggttattc
     6121 gatccgggaa acacggtccg tttcttggat gctcacagta tccggcgtgt gactacgtcc
     6181 gtcctctgaa atcttcagcg gatggacata ttgtcaaagt tctggagggg caggtttgcc
     6241 ccgtatgtgg cgcaaatctg gtattacgcc agggacgctt tggtatgttt attggttgca
     6301 gtaactaccc tgaatgcgaa cataccgaac ttatcgataa accggacgaa acagcaatta
     6361 catgccccca atgtcggacg ggccatctgg tccagcgccg ctcccgttat ggcaaaacat
     6421 ttcactcctg tgatcgctac ccggagtgtc aatttgccat taacttcaaa ccaatagctg
     6481 gagaatgccc tgagtgtcat tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa
     6541 aacacttttg tgccagtaaa caatgtggaa agccggtttc ggcggaataa taacgtgaat
     6601 aataacctgc aaggagacgc tatcgcagct gcgatagatg tcctcaatga aaaacgtgtc
     6661 atcgcctatc caacggaagc cgttttcggt gtcgggtgcg atcctgatag cgaaacagca
     6721 gtgatgagac tgttggagtt aaaacagcgt ccggttgata aggggctgat tttaatcgcg
     6781 gcaaattacg agcagcttaa accctatatt gatgacacca tgttgactga cgtgcagcgt
     6841 gaaaccattt tttcctgctg gccaggtcct gtcacctttg tctttcccgc gcctgcgaca
     6901 acaccgcgct ggttgacggg ccgctttgat tcgcttgctg tacgagtcac cgaccatccg
     6961 ttggtggttg ctttgtgcca ggcttatggt aaaccgctgg tttctaccag tgccaacttg
     7021 agtggattgc caccttgtcg aacagtagac gaagttcgcg cacaatttgg cgcggcgttc
     7081 ccggttgtgc ctggtgaaac gggggggcgt ttaaatcctt cagaaatccg cgatgccctg
     7141 acgggtgaac tgtttcgaca ggggtaacat aatggaaacc tatgctgttt ttggtaatcc
     7201 gatagcccac agcaaatcgc cattcattca tcagcaattt gctcagcaac tgaatattga
     7261 acatccctat gggcgcgtgt tggcacccat caatgatttc atcaacacac tgaacgcttt
     7321 ctttcgtgct ggtggtaaag gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc
     7381 cagagcggat gagcttactg aacgggcagc gttggctggt gcagttaata ccctcaagcg
     7441 gttagaagat ggacgcctgc tgggtgacaa taccgatggt gtaggcttgt taagcgatct
     7501 ggaacgtctg tcttttatcc gccctggttt acgtattctg cttatcggcg ctggtggagc
     7561 atctcgcggc gtactactgc cacttctttc cctggactgt gcggtgacaa tcactaatcg
     7621 gacggtatcc cgcgcggaag agttggctaa attgtttgcg cacactggca gtattcaggc
     7681 gttgggtatg gacgaactgg aaggtcatga gtttgatctc attattaatg caacatccag
     7741 tggcatcagt ggtgatattc cggcgatccc gtcatcgctc attcatccag gcatttattg
     7801 ctatgacatg ttctatcaga aaggaaaaac tccttttctg gcatggtgtg agcagcgagg
     7861 ctcaaagcgt aatgctgatg gtttaggaat gctggtggca caggcggctc atgcctttct
     7921 tctctggcac ggtgttctgc ctgacgtaga accagttata aagcaattgc aggaggaatt
     7981 gtccgcgtga atcaggccat ccagtttccg gacagggaag agtgggacga gaataaaaaa
     8041 tgtgtatgtt ttcccgctct cgtgaatggt atgcaactga catgcgcgat ctctggcgag
     8101 agtctggcgt atcgctttac tggagatacg ccagaacagt ggttagcgag ttttcgtcag
     8161 catcgctggg acctggaaga agaagcggaa aacttaattc aggaacaaag tgaagatgat
     8221 caaggctggg tctggttacc ctgatccaga tattcgtcct tccatttcac gtaattattc
     8281 gcggaatagc gtaacccagc cttctcttca tcacttaacg ggcggatctg tttgacgggg
     8341 ctaccgagat acagatatcc gctctccagc cgtttatttt gtgggaccag actacccgca
     8401 ccaatcatca catcatcttc tactattgcg ccatcaagta aaattgagcc catcccaacc
     8461 aaaactcgat tgccaatggt gcagccgtgg agcatcacct tgtgaccaac agtgacatct
     8521 tcgccaatgg ttaatgggtt gccatctggg ttgtacgagg atttatgagt gacatgcaac
     8581 atactgccat cctggatatt ggtgcgtgct ccgatctgta cataatgtac atctccacga
     8641 atcacaacga gcggccagat ccccacatca tcagccagac gaacgtcacc aatcacgaca
     8701 ctgctatcgt cgatcattac gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt
     8761 aaaacatcag acatacttac ctcagcaata aatgatttac taatgacttt gggggcatta
     8821 ttggccttgt gcaagtcttt tagtatgcaa aaaagcaccg ttttgtgtgc gattgcagca
     8881 aaaagggtga aaaaacaaca aacagaaaaa aagatcaaaa aaatacttgt gcaaaatatt
     8941 gggatcccta taatgcgcct ccgttgagac gacaacgtga aacacttcac aggatggtcg
     9001 gaacaacgaa gagaaaaaat cctgaaattc agggttgact ctgaaagagg aaagcgtaat
     9061 atacgccacc tcgcgacagt gcgctaaagc gcgtcgcaac tgctctttaa caatttatca
     9121 gacaatctgt gtgggcactc gaagata
//
LOCUS       DAJTOW010000139         9137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTOW010000139 DAJTOW010000000
VERSION     DAJTOW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.139"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..102
                     /locus_tag="OO244_003592"
     CDS             <1..102
                     /locus_tag="OO244_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5665281.1"
                     /translation="DGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            161..817
                     /gene="pdxH"
                     /locus_tag="OO244_003593"
     CDS             161..817
                     /gene="pdxH"
                     /locus_tag="OO244_003593"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5665282.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            946..2220
                     /gene="tyrS"
                     /locus_tag="OO244_003594"
     CDS             946..2220
                     /gene="tyrS"
                     /locus_tag="OO244_003594"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5665283.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2279..3142
                     /gene="pdxY"
                     /locus_tag="OO244_003595"
     CDS             2279..3142
                     /gene="pdxY"
                     /locus_tag="OO244_003595"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5665284.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3186..3791)
                     /gene="gstA"
                     /locus_tag="OO244_003596"
     CDS             complement(3186..3791)
                     /gene="gstA"
                     /locus_tag="OO244_003596"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5665285.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3897..5399)
                     /gene="dtpA"
                     /locus_tag="OO244_003597"
     CDS             complement(3897..5399)
                     /gene="dtpA"
                     /locus_tag="OO244_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5665286.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6010..6645)
                     /gene="nth"
                     /locus_tag="OO244_003598"
     CDS             complement(6010..6645)
                     /gene="nth"
                     /locus_tag="OO244_003598"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5665287.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6645..7340)
                     /gene="rsxE"
                     /locus_tag="OO244_003599"
     CDS             complement(6645..7340)
                     /gene="rsxE"
                     /locus_tag="OO244_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5665288.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7344..7964)
                     /gene="rsxG"
                     /locus_tag="OO244_003600"
     CDS             complement(7344..7964)
                     /gene="rsxG"
                     /locus_tag="OO244_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5665289.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7968..9026)
                     /gene="rsxD"
                     /locus_tag="OO244_003601"
     CDS             complement(7968..9026)
                     /gene="rsxD"
                     /locus_tag="OO244_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5665290.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2341 a         2287 c         2321 g         2188 t
ORIGIN      
        1 gacgggatct atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc
       61 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
      121 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
      181 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct
      241 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact
      301 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
      361 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag
      421 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
      481 ccttgaacgc caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
      541 gaaatatttt catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc
      601 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
      661 tcaacagggc gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca
      721 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
      781 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
      841 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
      901 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
      961 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
     1021 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
     1081 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
     1141 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
     1201 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
     1261 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
     1321 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
     1381 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
     1441 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
     1501 ctgaacaaac agtacgatgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
     1561 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
     1621 ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
     1681 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
     1741 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
     1801 ctagaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
     1861 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
     1921 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
     1981 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
     2041 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
     2101 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
     2161 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
     2221 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
     2281 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
     2341 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
     2401 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
     2461 cgaaattgtg caaggcattg ccgccattga taaattacac acctgtgatg ccgtattaag
     2521 tggctatctg ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa
     2581 agcagcgaat ccgcaggcga aatatttttg cgatccggta atgggtcatc cggaaaaagg
     2641 ctgtatcgtt gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga
     2701 tatcattgcg ccgaatctgg ttgagctgga aatactctgt gagcatgcgg taaataacgt
     2761 cgaagaagcg gttctggcag cgcgcgaact cattgagcaa gggccacaaa ttgtgttggt
     2821 taaacacctg gcgcgagctg gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc
     2881 cgatgaagtc tggcatatca gccgtccact ggtggatttt ggtatgcgcc agccggtagg
     2941 tgttggtgat gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca
     3001 ggaggccctg gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat
     3061 gcaggaatat gagctgcaag tggtggctgc tcaggatcgt attgccaaac cagaacatta
     3121 cttcagcgca acaaagctct gaaatatctc atttcggccc acaacgacct gtgggccgat
     3181 ggctattact ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata
     3241 cgttgcataa atgccgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc
     3301 cagcgcagaa ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag
     3361 tgctcatcct tcagtgcctc gttcacatat tgcagcttct tatccagctg cgcgcgaact
     3421 gtcggtttgt actcttccgg tgtatctggg cgaaacagag gtgtgaaacc tttatgcagc
     3481 tcggtggcga tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt
     3541 gccagcaact ggcggtcggg gacgctgtcg gcaagatact gcataatcgc aacgccttcc
     3601 gtcagcaaag taccatcgtc cagcagcaat gcaggcacct gccccttagg gttaacggaa
     3661 aagtaatcgt caccgttttc gagacgtttt ttcattaaat ccacactgac gagcgtaaaa
     3721 tcctttccgc tctcacgcag ggtgatatgg gaagcgagag agcaggcgcc cggtttgtag
     3781 aacaatttca tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa
     3841 caaaaaaaaa gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac
     3901 gctacagtag ctttcgccgc tttgtctgca gcgtcatcct gcgtcatgcg gtgcagttta
     3961 ggcgcggtca gcagcatcag tactgcaata acggcagtag cgacaccaat ctgcaagaat
     4021 acgcgaccat agacttccag tgacatcagc gggtcggtaa cgttatccgg cacagccatc
     4081 atacccgcaa cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc
     4141 ataatgaagc ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca
     4201 gagatcatca gttccccgat gctctgcagg ccatagcttg cgaccagcca gctgacagac
     4261 acgatacctg cgtcagacgc gaatttcgca cccagcggca gaatcaggaa cgcaccagaa
     4321 cacatcacca tgccgattgc aaacttggtt ggcatcggca gggtatcacc catcttgtta
     4381 tagatagcgg ccagaatcgg actaccgatg atgatccaga acgggttcag tgcctgatac
     4441 tgctcaggtt ctacggccag acccagaatg gagtgctcaa cgttacgaat cgcaaagaag
     4501 ttcagtgacg ttggcatctg gctgtacagc acgaagaaga tgattgcttc gagcatcagg
     4561 atgaaggcaa cgatcatttt acgacgcgca gcacctttca tcgcgaaagc ttctttaccg
     4621 aagataacca caataccgaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg
     4681 tgcagtagcc aggtggcaac agcgatcagc gccacaacac caataatggt cagcaacagg
     4741 ttacggtagt tgactggttc aaagtctggt tttgaaccgt actgtttaac ccagcgttgg
     4801 cagaaggcga agttaacgat agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca
     4861 ctccagccgt atttcgcggc cagccacggg gtggctatca tagagaagaa agagccgatg
     4921 ttgacggaca tgtagtacat ggtgaatgca ccgtccagac gtgggtcgtt tttctcatag
     4981 catgtagaaa gcagagaaga cgggttagct ttaaacaggc cgttaccgac cgcaatagcc
     5041 gccataccca tataaacaat accggcgtcg tgaccagacc aggcaaccag agcataacca
     5101 atcgccagca caatagcgcc gagcataatt acgcgtttag tacccagtac cttgtcacct
     5161 aaccagccgc cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg
     5221 attgaatccg cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt
     5281 aggccgtaat aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc
     5341 ggttgtttga aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact
     5401 tttacctctt tttttacatc ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa
     5461 acatacgcgc aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac
     5521 tttcagacaa gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat
     5581 tccgggaaat gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact
     5641 gcattgagag ggggatgatg gcttggcggc gatatgttct gctatttagc gtttttccta
     5701 gtgagatagt ccagtttctg aaaaatagcc agtgtaatgt tttgcaggtc aatatttgtt
     5761 gcgtgtgtaa atttaaatca ttttaacctg attcttgcaa tgtttttaca ttgccacagc
     5821 atcgtgatct tgatcacgaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc
     5881 atttttggtt atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac
     5941 gttaaaaaga tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac
     6001 cccttttctt cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga
     6061 gccacagcgg ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg
     6121 gcagtcgact ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac
     6181 gtttttcccc ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc
     6241 aatagtcggc cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc
     6301 gggcagggct tcaagcgcag cacgatcttc cggaacctcg ccattatgtt gttccagtaa
     6361 tatacggcag gttttgatga tattttctgc tttgctgtta taaaggccaa tcgttttgat
     6421 ataggttttc accccttcaa cgcctagttc aagcatcgct gcaggcgtat tcgccaccgg
     6481 gtagagtttc gccgtcgcct tattaacact gatatcggtc gcctgagcgg aaagcagtac
     6541 ggcaatcagc aattcaaaag gcgaactgaa attaagctcg gtggtgggat gaggattgtt
     6601 ctcacgcagg cgagtgagga tctccagacg ttttgctttg ttcatcagac attccctgtt
     6661 tcaccgtttg gcaatgcacg ttcggcagct gcttcagcac ggcgcttttt cattctttca
     6721 tcaatcaggt attttcctgc cagcatcagt cccaagccaa taaatgcacc tggtggcagc
     6781 atcgccagca ggaaagggga gtcggtgtgg aaaatctcca cgcgtaatac ttttgcccag
     6841 ctacctaaca gcgcatctgc accgtcaaac aacgtgccat tgccgataat ttcgcgtagt
     6901 gaacccagca cgaacatggc gcaggttgcg cccgtaccaa ttgaaaagcc gtccagtgcc
     6961 gaaagcgccg gacctttttt ggcggcgaag gcttcagcgc ggcccacaac gatacagtta
     7021 gtgacaatca gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg
     7081 atcagcatct gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg
     7141 atctcggctg gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc
     7201 agcgtagtcg caagtcctaa acccagagcg ttagtggcag tggatgtgac cgccaatagc
     7261 ggacaaaggc cgagcagttg gaccagtgca gagttgtttt tccacaaccc ctgaacaata
     7321 acgtctttaa tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgtgcc
     7381 ggtaacgtct gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta
     7441 atcgtcgcgc cggtgaactg gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca
     7501 tcatccgcac cactgatttt ttttcccgca aaatgggtga gccagtcaga aaggcgcaat
     7561 tcgattttat cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc
     7621 gtgccattaa aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg
     7681 gttgcttcca gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc
     7741 tcacctttac ctaactctgg cgcagttacc agatagcaac tctgtgcgag cgcattgtta
     7801 tagcgttcgg ctggcagcac ctgatcaaat aacgtctttt gttgcagact ggcctgttca
     7861 gcaattgtcg ttttggtcat ctggttgatg gccgcagtta accctgtaga acccgctgca
     7921 aatagtgcca gtgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga
     7981 tggccgtaga cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc
     8041 aggacggcaa aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat
     8101 aagcccgcca gcgcgccgaa catcagccga ccacgattgg tcgtagaagc agtaactggg
     8161 tcagtcaaaa taaagaatgc gccgagcata gtcgcaccag acagcagatg aatttgcggt
     8221 gctgccagtg tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact
     8281 aagaagctga ggggaatatg ccagcgaatc gctttctgcc atagcaacca cacgccacca
     8341 gccagccagg cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc
     8401 ggatattgca taatctgttc aaccgaatga cctgcgcgga cagaggtttt gaatgtatcc
     8461 agcggtgtcg cctgactaat gccatcaata cctaagcgta gcgtattcat atcaccacca
     8521 ctggcggtat gaccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc
     8581 gcaatttcat gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc
     8641 acataaccaa tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta
     8701 gcgatgatca ccgcaaacac cgtacccagt acgaccatcc accatggcgc taggggggga
     8761 atacttaccg ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgttgcggct
     8821 accgactgct tgcgtagttt gagtacgaga gcttcggcta acagagcact gaccgacgcc
     8881 aacaggatct gaacgagagt accccaacca aaaaaccaca gttgcgctgc gattcctggc
     8941 acggctgcga gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa
     9001 ggggagctag ctattctgaa taccatttag tcctcgttta caaccttttg ctgggcggct
     9061 tttttggcct gaacgcgggc aatagccgcg gcaactgcgg ctttgcgcgg atcgacctgt
     9121 tcttctggtt ccgcatt
//
LOCUS       DAJTOW010000140         8998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTOW010000140 DAJTOW010000000
VERSION     DAJTOW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.140"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..711)
                     /locus_tag="OO244_003602"
     CDS             complement(<1..711)
                     /locus_tag="OO244_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5665291.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGS"
     gene            771..1049
                     /locus_tag="OO244_003603"
     CDS             771..1049
                     /locus_tag="OO244_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665292.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1050..1577)
                     /gene="gadE"
                     /locus_tag="OO244_003604"
     CDS             complement(1050..1577)
                     /gene="gadE"
                     /locus_tag="OO244_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5665293.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2376..2948)
                     /gene="hdeD"
                     /locus_tag="OO244_003605"
     CDS             complement(2376..2948)
                     /gene="hdeD"
                     /locus_tag="OO244_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5665294.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3203..3535
                     /gene="hdeA"
                     /locus_tag="OO244_003606"
     CDS             3203..3535
                     /gene="hdeA"
                     /locus_tag="OO244_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5665295.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3651..3977
                     /gene="hdeB"
                     /locus_tag="OO244_003607"
     CDS             3651..3977
                     /gene="hdeB"
                     /locus_tag="OO244_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5665296.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4041..4687
                     /locus_tag="OO244_003608"
                     /pseudo
     CDS             4041..4687
                     /locus_tag="OO244_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4729..5259)
                     /locus_tag="OO244_003609"
     CDS             complement(4729..5259)
                     /locus_tag="OO244_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5665297.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5415..5981)
                     /gene="slp"
                     /locus_tag="OO244_003610"
     CDS             complement(5415..5981)
                     /gene="slp"
                     /locus_tag="OO244_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5665298.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6228..7451)
                     /locus_tag="OO244_003611"
     CDS             complement(6228..7451)
                     /locus_tag="OO244_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665299.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8032..>8998)
                     /locus_tag="OO244_003612"
     CDS             complement(8032..>8998)
                     /locus_tag="OO244_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5665300.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLTTKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMELI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2640 a         1775 c         1870 g         2713 t
ORIGIN      
        1 actgccctga acctgtttga tttgcccact ggcgacctct tctttcatgc gtaagaaatc
       61 ttgcaccgac tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga
      121 atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc
      181 cgtaatcggc gaggtgacat tcgcgtattg cagattgatc gtcgcctgtt caacagccgc
      241 tttggcgacg gtgacattgg cttctgcttc attcagctgg gtgcgcgcgg tgtcgtaatc
      301 ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc
      361 attgctggcg gtagagagcg ctttcgccag cgagcctttg gcggagttta gctcggcctg
      421 taaaggtgca ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat
      481 aaagttgcgt ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg
      541 aacggttcta ccgggcaatt cgctcaacac attgaccgaa ccaggggaga gtgtgacgac
      601 accgacctca ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag
      661 catcgcgccg cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc
      721 tgaaaattct tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt
      781 tacagaccag cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc
      841 gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca
      901 ttcgggcaac gaagggttct actggtggat acacatacca ggggaatcaa gtcgttttag
      961 cctctccttt agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca
     1021 gccatcaatt tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg
     1081 atgtcgctca tacgtcttag tttcagcttg tacataatgt tgaatttctg aatctttaat
     1141 gtttgttgcg aaatattgag cgtcgacgtg atattgcttt catttttatt tttcagaatc
     1201 agttgcataa ttttgaactg attatgtgaa aaacataatt gtgaattctt atggggcaag
     1261 tgtttaccat aagtaatctc aagaagattt ttgatacttt ctttgcggct aacaaaataa
     1321 ataggcgctt tagcttttag tttactgatg tgataaggtg ccaaaacaat aattctctcg
     1381 gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt gtgataggta
     1441 tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag ggaattgatt
     1501 tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa agaatctttc
     1561 gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat gtcatccttt
     1621 tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta taagaacttt
     1681 gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt atccatcacg
     1741 cactccatat attgatccta agtaagattc ctggttgtta tcagcttgta aatagtaaac
     1801 aaaaagttaa cgtttgcgga ttactgccca agaataagtt atcaataacc agtttgtgat
     1861 ctctgaagaa tattactaaa gttaaaatgt aatccgattt aaatatcgag tctccttgtt
     1921 tcgacttaag ctggcaattg gattgccagc tttcttttaa cgcattgaaa ttacctgagt
     1981 aaataacgca ttcatgtctt caggttttta tatagcgtct ggagacacgg atatttatgc
     2041 aatgtaatca acgccagttg caaaaaaatg aacccggatt acttatttaa ccgcccatag
     2101 catcaacgct tacggaactc tttgttcact ttcccacagc acttttaaaa cattaaacct
     2161 gactacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca tggagtgaaa
     2221 aaaattagat gaaattcagt aggttgaaat aatcactagc aggtaattat ttcaatgata
     2281 gtgcgcaatt gatctacaac actgcgtagc ggagagagta ttaatcggat catagtcaca
     2341 tcaagtgact atgatccggg tgacaaccgg ggtaattatt gctgcttaac gaacaaactg
     2401 gcgaagctga acaggctggc ggcgctaaat atcagttcaa ttcccaccag tgtggaaacc
     2461 agcgttacag acaccatcgg cgttgcacca aggaatatcc aggcaatgac gatatccagc
     2521 acaccaataa cgagctgtag ccagctgcct ttcattgaac gctgacgata ccaactcatc
     2581 aggcgaataa cccctgcaac acagaacaaa ccggcaataa atgccgcaat ggcaaaaatg
     2641 cccagctccg gtgcacggat gaagaaatag ccgatcaata aataggcgac tgcgacgagg
     2701 aaaccggata ataccggcca gaaattatga ctgcggttgc tgaataaccc gacaataagc
     2761 gcaatacccg agcagattaa taatgcaccc actactgtgc ttaaaatatc gccagagacg
     2821 aacgggaaac tgatacacag caacccgacg ataaacagca gcacggcaat aaactggatt
     2881 gctctgcgat gctttttcag catctccaga tcaaacttca aaattgttgc cttatctata
     2941 tataacatag aaccacccta taaaattaag aagaaaatcc cctgctatca atctatgcca
     3001 aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc ctaattgcag tatctgatgc
     3061 atctgtaact cattgtattg aaataaaaat atctgatttt gatattttcc atcaacatga
     3121 catatacaga aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac ggctctttca
     3181 ctttatagtt gaggatatta cgatgaaaaa agtattaggc gttattcttg gtggtctgct
     3241 tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata acaaaaaacc
     3301 ggtcaactca tggacctgtg aagatttcct ggctgtggac gaatccttcc agccaactgc
     3361 agttggtttt gctgaagcgc tgaacaacaa agataaacca gaagatgcgg ttttagatgt
     3421 tcagggtatt gcaaccgtaa ccccagctat cgttcaggct tgtactcagg ataaacaagc
     3481 caactttaaa gataaagtta aaggcgaatg ggacaaaatt aagaaagata tgtaattccg
     3541 ggaatgcgtt acatcgtact tccttgcata ttgaacaggc cggaatatct tctttaaaag
     3601 cagctattcc tcctgttcat atataatctc tatattgaat gggttacaaa atgaatattt
     3661 catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtca ctggtgaacg
     3721 cacaatctgc gttggcagcc aatgaatctg ctaaagatat gacctgccag gaatttattg
     3781 atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa gaaacagtat
     3841 ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt cctaaagtga
     3901 tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat caagcatcta
     3961 atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag ccatcttgct
     4021 gctccttttt gcatttttat atgacagcag aatttattat acgtcttata ctcgcggcaa
     4081 ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca gggttacgca
     4141 cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa tatggttttg
     4201 ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct gctcaggtgg
     4261 tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa aatattgtag
     4321 gtctgacgac ggcagcggat atctgggtga ccgccgcata ggtatggtta ttggcagcgg
     4381 tatgtacgaa ctgggtattt atggttcagt gatgaccttg ttggtgctgg aagtcttcca
     4441 tcaattaacc ttccgcctga tgaacaaaaa ttaccattta cagctgacat tagtgaacgg
     4501 taatacggta tcaatgctcg actggtttaa gcagcaaaaa ataaaaactg atttagtttc
     4561 gttgcaggaa aatgaagatc atgaagtggt cgccattgat atccagcttc atgccacaac
     4621 gttgatagaa gacctgttgc gattattaaa aggtatggca ggggtgaaag gcgtttcaat
     4681 tagctaatct gtgtggcagc gtagccagac tcaccgtaag cctgaaattc acaccagata
     4741 atcaatatgc tgatggcgta ccagctcatt gatccgctta agattgagga tcatcatgat
     4801 attgtgttta tggcagtaga acgtttttag cgatattttc agtttctcca gaatatcttc
     4861 agttgtcatt tgattcgcaa tgcagctcag aacctgatgt tgcttgcgcg aaagtgacac
     4921 attcgcttca ggcacagagc agtaactgcc attgatgagt gcgacaaaat caatgatagt
     4981 gatagtccgc ggaacaaagg tcaccggtac tttccccaga cagcgtttaa tattaaaggg
     5041 ggagaaaaca atgacatgga caggttttaa tcgttcaatt tgagtgagaa aattagaatg
     5101 aaatacatta ttcattaatg tatcaataat gacgaaggca ttctgatgca acattacgtc
     5161 gatctcttct tcgttctgta tatgaacgac attaccttta ctcagaatgt ttttcatcgc
     5221 ggtgaagaac atcgtatccc tggtaattat aagaaacata accgactcct ggtgcggaca
     5281 ttaactttat aatcagtttg ccaaaggtaa ttaacaggac agggcttata ataataatag
     5341 ttagcttact gtatattaat tttatgtaat acatgatgca gatgctgaga aagcatctgc
     5401 atctttcggt ggtgttattt gaccagctca ggtgttacct gactcaccgc attggtgtag
     5461 taaggcgcac cccagcccgg ttccggccag aatgcaccat agccgtaatc ccacaggtta
     5521 taggtggtat taaccacttc tctcaaatgc cacacctgga tgccctgcat attcacttcc
     5581 aggaagttat acgggacttt attgataaag cctggttgtt caccctgaat ggtaccgagg
     5641 atggtaacaa agtgattacg atagttcact ggatcaagga agccgctttg tctggcgagc
     5701 agtcggccct gatagttggc ttcaatatca ggcttcgcat agctatccaa cggtaataca
     5761 gcgatttcta acaacgtatc cgttttgcca ttgataacgt tgataacctt cccaccaaag
     5821 cgcgcttgtt gaccaacata taaccccggc tggttatgaa cagcaacaaa acttttttga
     5881 atatcaggtt ggttattgcc tttgatattt tgcggaattg aactacatgc ggcaagcaaa
     5941 aatgaaaggc tgaggatgag tgcacctttt gtcatgttca tgttactatc cttatcaaca
     6001 aatattaaaa ccataataga tatttcttta tagttttcat ctgattctga ggtgatatgc
     6061 ctgatagtaa cgtaaacctg tctgatgttt atatcccttc tgtttagttc aattccgtta
     6121 ttttggggcg tggtatgagt atccatcgcc caataatata accctttgtt ttaaaattac
     6181 ataaaatcta tttttattca atgattataa agtgctaata atcaatatta tggtattata
     6241 ttctgataat gatttgataa gcgaattctt ttgccagatg gtatttctat ttccacccca
     6301 taatttatat ctttttcatt aggcagatct ctctttaaat aaaaggggta tgaaatcttt
     6361 tcgggttgaa gtgtctcgta atgaaattct atgaattcat ttttagacat tgttgcccat
     6421 ttttgaattt tattgtatag agagggtgtt gtaaactctt gaagttgttt ataacatatt
     6481 tttttgaact cttgcttata ttttaatatg tcatcaaatg aaagattcac tacctttgcc
     6541 tctaagaaat caattattct cgaagcaatt tccgctttcg cgcgtaaaga gttgtcatta
     6601 atagttaaca atgtagaaaa caaaccattg atttgtttgt caagatgttg ttcatattta
     6661 tgtagtttgg ggatgggatt ttccttgtgt gaaaaaatag tgttaaataa atttgcgtca
     6721 gtgaaattta tattaagtag atcttcaaaa taaaattttg ataaggttag gactagcttg
     6781 gacatatctt gccctgtcat gttgaaatca ataaagtcgc atccagcaag ggcttccatc
     6841 ctattttcat ttttactgtg ctcaatatac agcttcgcca tttttaagta caagctttga
     6901 atgtctctta tatcagttgg taagtcagct cgttgcccag caaaattttt attatcggaa
     6961 gaaacggttt ggacactgaa tctttcgggt attccttctt ctacgagaat tttagttatg
     7021 attggattgt tgggttctgg attaacgaca acaatgattt gacatacaga atccttatcc
     7081 ttttcgtatc taatgttatg aatctgatta agtattgatt gtgtctgttg cgtaagtggt
     7141 gtcgaactta tcgtttcaat gttattaata agacttacaa gttgaaccac aacttttgag
     7201 tagtctgcat ttgtatatgt ggtgttcgtg tggcatgcat taatttgttg gagttgttgt
     7261 tgcgctaaat caaattgttc agggttgtat ccttctccag agaatgtgga attaatgggt
     7321 tgttgttctt cccaattatc acaaggccac ttcggtgtgt ctggcgtttt aacattatcg
     7381 gtatttgttg tgcagttata aatatcgccg tgatatgtat ttattatacc tggggtaaag
     7441 tcaattgaca taacattcct tattcatcat ctgttttatg ctaatgtttt taaattggat
     7501 aattagaaca ggaatggatt ttcttctaca cacaataaat ataatgcgtt atatcttcct
     7561 gatataatta cctttaaatt ccctttcatt tccacccggt aatatttgtg tataaaaaaa
     7621 gggcagtgat agtatcgtaa aggaaaacgt taacaactat caaaaaacgc tcattttaaa
     7681 aagttattat gttctccgat agtgtgtgaa gtaatatttt tgtgatgttt tagatgtgta
     7741 ataatgaacc aggttaaagt gtatttaaaa atgtgttgta ttagcgactg aaatctgcct
     7801 tttaaaaagg aggagggtac tgagacaaag agtcattgct tctccgataa aatgtcagag
     7861 aagcatgatt acgtggttat tgtctgttga tgacttgttg accgagccgt catcattctc
     7921 aatgcattag ttaaatcatt tgtgattttt aaggattatc ctgtctatga ttttctactt
     7981 agaaggatat ggcactaact atgcatctta tagtttactg ctggcatcta tttaaaatag
     8041 caattgagtc aaatagccca tcaagatagc catacctaat ataaccgtca ggaatgcagc
     8101 tatcatcggc attctgaaca ttgatttaag taatatcact tccgtcaggc ttgcgccggc
     8161 actgccgatg attaattcca ttaatgctcc cagacccatt ccttttgtca tcagaacaga
     8221 tgccagaggg ataaccgcct cagcccggat atacagcgga ataccgacaa cggcgcttaa
     8281 tgggatggcg aagggattat ctgctcctgc atgagcggca atccacgctg agggaataaa
     8341 gccataaata aaagatccta ttaatactcc taacaaaaga tatggcagta catctttaaa
     8401 ctgttgcaac gcatctttgg tggttagttt tatgggatta atacttacta cagctttggt
     8461 actgcaacag gtaactacgg ggttctcaat ggctttagcc gctggactac aacaactcac
     8521 ttttatgggc gtatatgtcg ttcccggcga agtttttgct ggagcacaac aatttgctga
     8581 tgagctttta ctggcaatga tatgacgttc aaaccccaga gaatccagga taatactggc
     8641 aagaacggag acgccggctg cgataatcgc gtacaacaag gtgactttcc aaccaaaggt
     8701 catccacatt agtccgacga taatgggatt aagtaatggt gaaacaaata aaaaagtgag
     8761 agtcggacca aacccggctt tcgctgacaa caatccacgt aacatgggta ttgtcgagca
     8821 actacagaac ggggtaacgg ctcccaacag agcagccagg agatagcctc tcccttttct
     8881 ggcccccatc atctgctgga ttttatggtc tggcaccttt tgtcttatca ggctgacacc
     8941 ggcactaatc actataaaca gcaacgaaag ctcgacggcg agaaacacaa acatctct
//
LOCUS       DAJTOW010000141         8996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTOW010000141 DAJTOW010000000
VERSION     DAJTOW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.141"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO244_003613"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO244_003613"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5665301.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASRVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO244_003614"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO244_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5665302.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OO244_003615"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OO244_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5665303.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO244_003616"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO244_003616"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5665304.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO244_003617"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO244_003617"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5665305.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OO244_003618"
     CDS             complement(7826..8137)
                     /locus_tag="OO244_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5665306.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO244_003619"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO244_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5665307.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8649..>8996)
                     /gene="csgA"
                     /locus_tag="OO244_003620"
     CDS             complement(8649..>8996)
                     /gene="csgA"
                     /locus_tag="OO244_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5665308.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2250 a         2330 c         2134 g         2282 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccagacgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accctactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tgattttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgttgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctaacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa catcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgcaccaaa ataggcatca ccaatatttc gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgcaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta actataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttccccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
     8701 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
     8761 acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca
     8821 cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc accattacca
     8881 ccgccatgct gggtaatagt caagtcagag ttacgggcat cagtttgcag agcaagtgca
     8941 gagttaccgc caccgtactg gtaaatgttc agctctgaat ttgggccgct attatt
//
LOCUS       DAJTOW010000142         8994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTOW010000142 DAJTOW010000000
VERSION     DAJTOW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.142"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..2887
                     /locus_tag="OO244_003621"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OO244_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO244_003622"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO244_003622"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5665309.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMAPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OO244_003623"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OO244_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO244_003624"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO244_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OO244_003625"
     CDS             complement(7280..8041)
                     /locus_tag="OO244_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5665310.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OO244_003626"
     CDS             complement(8038..8697)
                     /locus_tag="OO244_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5665311.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2189 a         2388 c         2345 g         2072 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg ccatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctgg tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt gtcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacactgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatgacagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatctaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtttcc cccgttactt tattcgtacc ccttataatg gggtgttagc
     8941 cagccagacc cggcatgatt actgccccca gtcgtccatg atccgggggg tgat
//
LOCUS       DAJTOW010000143         8751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTOW010000143 DAJTOW010000000
VERSION     DAJTOW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.143"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            5..436
                     /locus_tag="OO244_003627"
     CDS             5..436
                     /locus_tag="OO244_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5665312.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO244_003628"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO244_003628"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5665313.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO244_003629"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO244_003629"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5665314.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO244_003630"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO244_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5665315.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO244_003631"
     CDS             complement(4438..5145)
                     /locus_tag="OO244_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5665316.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO244_003632"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO244_003632"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5665317.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO244_003633"
     CDS             complement(6003..6206)
                     /locus_tag="OO244_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5665318.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO244_003634"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO244_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8751
                     /locus_tag="OO244_003635"
     CDS             7870..>8751
                     /locus_tag="OO244_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5665319.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGI"
BASE COUNT         2029 a         2385 c         2249 g         2088 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat t
//
LOCUS       DAJTOW010000144         8694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTOW010000144 DAJTOW010000000
VERSION     DAJTOW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.144"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..104)
                     /locus_tag="OO244_003636"
     CDS             complement(<1..104)
                     /locus_tag="OO244_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5665320.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO244_003637"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO244_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5665321.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1353..2003
                     /gene="csgD"
                     /locus_tag="OO244_003638"
     CDS             1353..2003
                     /gene="csgD"
                     /locus_tag="OO244_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5665322.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2008..2397
                     /gene="csgE"
                     /locus_tag="OO244_003639"
     CDS             2008..2397
                     /gene="csgE"
                     /locus_tag="OO244_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5665323.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2422..2838
                     /gene="csgF"
                     /locus_tag="OO244_003640"
     CDS             2422..2838
                     /gene="csgF"
                     /locus_tag="OO244_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5665324.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2865..3571
                     /locus_tag="OO244_003641"
                     /pseudo
     CDS             2865..3571
                     /locus_tag="OO244_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3704..4195)
                     /locus_tag="OO244_003642"
     CDS             complement(3704..4195)
                     /locus_tag="OO244_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5665325.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OO244_003643"
     CDS             complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OO244_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5665326.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OO244_003644"
     CDS             complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OO244_003644"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5665327.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OO244_003645"
                     /pseudo
     CDS             complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OO244_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6840..6927
                     /locus_tag="OO244_003646"
     tRNA            6840..6927
                     /locus_tag="OO244_003646"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Ser,seq:gga)
     gene            complement(7120..8106)
                     /locus_tag="OO244_003647"
     CDS             complement(7120..8106)
                     /locus_tag="OO244_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665328.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(8103..8631)
                     /locus_tag="OO244_003648"
                     /pseudo
     CDS             complement(8103..8631)
                     /locus_tag="OO244_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2476 a         2090 c         1856 g         2272 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccaccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaattgt ccacggaggt
      961 gtggagaaaa aacaaaaacg ttttacatga cgaaaggact acaccgaaat attttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata
     1141 taatgagtta cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt
     1201 gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat ttattactac
     1261 acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag
     1321 gtgtgcgatc aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg
     1381 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc
     1441 ttaaacaatc gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
     1501 tctcttcagg ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt
     1561 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
     1621 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
     1681 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct
     1741 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
     1801 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
     1861 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
     1921 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
     1981 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
     2041 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
     2101 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
     2161 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     2221 tggatcacaa taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa
     2281 agagactccg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     2341 cgccagataa atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata
     2401 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     2461 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     2521 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     2581 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     2641 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     2701 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     2761 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     2821 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     2881 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     2941 gaccgacatt aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga
     3001 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     3061 acccggcaag taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct
     3121 taacgagcgc aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
     3181 aatcccgctg caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta
     3241 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     3301 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     3361 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     3421 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     3481 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     3541 tggtatcgac cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct
     3601 ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt
     3661 aataatccgg catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
     3721 tttcctgctc acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt
     3781 tcattgcgta accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac
     3841 cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca
     3901 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
     3961 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
     4021 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt
     4081 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc
     4141 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
     4201 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa
     4261 attatctgtt gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag
     4321 ctcgtcccac attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg
     4381 cgtggttgca tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc
     4441 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
     4501 agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg
     4561 ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata
     4621 tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
     4681 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
     4741 agccagtttc ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg
     4801 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
     4861 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
     4921 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc
     4981 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
     5041 gagcctaatg ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg
     5101 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
     5161 tgtttcgcgg ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
     5221 tggcttatta tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc
     5281 gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag
     5341 tcaaacattt taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg
     5401 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
     5461 cagtgatgcg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
     5521 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
     5581 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
     5641 aggatagcag gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag
     5701 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
     5761 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
     5821 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
     5881 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
     5941 agatacgcgc catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
     6001 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
     6061 tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
     6121 agcggaaagc gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc
     6181 aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta
     6241 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
     6301 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
     6361 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
     6421 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
     6481 acataatcag cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata
     6541 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
     6601 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
     6661 caaaggccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt
     6721 aaaaccacac attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga
     6781 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     6841 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     6901 ggggttcgaa tccccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc
     6961 ttttttttcg tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga
     7021 aattgtgcac atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca
     7081 gaacatatat gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca
     7141 acggaaatac atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa
     7201 aaaatagtaa attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg
     7261 ccattccaca agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg
     7321 caatatgaat aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact
     7381 ggagaaaaac agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga
     7441 gaagcgcaga aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat
     7501 tatcgattta aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg
     7561 gctgatagta agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt
     7621 cagactttta tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt
     7681 gcggatcatc cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag
     7741 cgcatataac gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa
     7801 cgacagaaat cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt
     7861 tttcttaaac cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt
     7921 gagatcaaaa ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc
     7981 ctctgatgga aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat
     8041 agcataaatg tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg
     8101 aatcatttga atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga
     8161 tccacgccag caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg
     8221 aaagctaccg gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt
     8281 atgagttaag tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc
     8341 tgttacgttc atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc
     8401 gcgcattgcc tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag
     8461 tttaaccggt tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg
     8521 cgttccccca ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc
     8581 tgattccgta atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta
     8641 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gact
//
LOCUS       DAJTOW010000145         8689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTOW010000145 DAJTOW010000000
VERSION     DAJTOW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.145"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..542
                     /locus_tag="OO244_003649"
     CDS             <1..542
                     /locus_tag="OO244_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5665329.1"
                     /translation="NYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLAAAVGMWVN
                     GIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMALFCVHF
                     AGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLALVIAA
                     CVTFTGALCYFFIVKDRIE"
     gene            794..1711
                     /gene="oxyR"
                     /locus_tag="OO244_003650"
     CDS             794..1711
                     /gene="oxyR"
                     /locus_tag="OO244_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5665330.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1694..3094)
                     /gene="sthA"
                     /locus_tag="OO244_003651"
     CDS             complement(1694..3094)
                     /gene="sthA"
                     /locus_tag="OO244_003651"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5665331.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3415..4065
                     /gene="fabR"
                     /locus_tag="OO244_003652"
     CDS             3415..4065
                     /gene="fabR"
                     /locus_tag="OO244_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5665332.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4065..4424
                     /locus_tag="OO244_003653"
     CDS             4065..4424
                     /locus_tag="OO244_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5665333.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4464..5564)
                     /gene="trmA"
                     /locus_tag="OO244_003654"
     CDS             complement(4464..5564)
                     /gene="trmA"
                     /locus_tag="OO244_003654"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5665334.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5678..5868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5933..7777
                     /gene="btuB"
                     /locus_tag="OO244_003655"
     CDS             5933..7777
                     /gene="btuB"
                     /locus_tag="OO244_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5665335.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7722..8579
                     /gene="murI"
                     /locus_tag="OO244_003656"
     CDS             7722..8579
                     /gene="murI"
                     /locus_tag="OO244_003656"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5665336.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1994 a         2108 c         2327 g         2260 t
ORIGIN      
        1 tcaattatac cggttggctt tacattgcgt ggttacccgg ttatttgcaa gccgaacaag
       61 gtttcagtct ggcgaaaacc ggttgggtag cggcgattcc tttcctcgcg gcggcggtcg
      121 ggatgtgggt taacggtatt gtggtcgatc ggctggcgaa aaaaggctac gacctggcga
      181 agacgcgtaa aacggctatt gtctgcggtt tgatgatgtc ggcattaggc acgttgctgg
      241 tcgtgcaatc ttcctcgcca gctcaggcgg tagcgtttat ctcaatggcg ctgttctgcg
      301 tgcatttcgc gggaacgtct gcatggggac tggtgcaggt gatggtgtca gaaacaaaag
      361 tggcttccat cgccgggatt caaaactttg gcagttttgt ttttgcttcc tttgctccga
      421 tcgtaaccgg ttgggtagtg gataccacac actcgtttaa tctggcgctg gttatcgcgg
      481 cctgtgtgac gttcaccggg gcgctgtgtt acttctttat tgtcaaagat cgcattgagt
      541 aatcgttact atcaggctct cttgctgtgg gcctgtagaa taaaaaaaag cggatcctgg
      601 agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc
      661 agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa
      721 tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga
      781 tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg
      841 ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta gcggccaaat
      901 tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt
      961 gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa
     1021 agtccttaaa gagatggcaa gccagcaggg tgagacgatg tccggaccgc tgcatattgg
     1081 tttgattcct acggttggac catacctgct accgcatatt atcccgatgc tgcaccagac
     1141 atttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact
     1201 ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat
     1261 tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagatc acccgtgggc
     1321 gaaccgcgaa tgcgtactga tggccgatct ggcaggggaa aaactgctga tgctggaaga
     1381 tggtcactgt ttgcgcgatc aggcaatggg tttctgcttt gaagccgggg cggatgaaga
     1441 tacacacttc cgcgcgacca gcctggaaac tctgcgcaac atggtggcgg caggtagcgg
     1501 gatcacttta ttgccagcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta
     1561 tctgccatgc attaagccgg aaccatgccg cactattggc ctggtttatc gtcctggctc
     1621 accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa tggatggcca
     1681 tttcgataaa gtgttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt
     1741 ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac
     1801 ctttctgttc cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc
     1861 agtgaatacc cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt
     1921 tcatgccgac gatttgtgca cgtgccagat gtttgaactg ggcgcggccc acttcatacg
     1981 gcactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg
     2041 tgtaaatgcc ggtagggata tcttcaatca gatgtgcggt ggcttcgcct tttaccagcg
     2101 cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca
     2161 cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct
     2221 gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg
     2281 tacgaccgtt ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcagatgca
     2341 tgatcacacc gtcgtcacag ctttcgatct tctcgtactc ttcgttgtga cgaatcacta
     2401 cgccactgtt ccagaagtgg taggagagag aatctgacat ctcttgatcg agaaatgcca
     2461 gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg
     2521 catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca
     2581 tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat
     2641 gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc
     2701 cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata
     2761 tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt
     2821 tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt
     2881 atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg
     2941 ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga
     3001 cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta
     3061 ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat
     3121 aaaagcaaca gaatggtaac attctatcgc gggtaagcca attgatcccc gtcatttatc
     3181 tggctatatc ctgagcggcc tttgctttgt ctgtttctta cttttgccct gacgttttat
     3241 tggattttta tcgacgatac tctccgttta agcggcaggt ttccgctgta cgtaaaagaa
     3301 ccggccagta aatatgtttt attgcgttac cgttcattca caatactgga gcaatccagt
     3361 atgttcattc tctggtatag tgccagcagt acttttggca aggattcaga catcgtgatg
     3421 ggcgtaagag cgcaacaaaa agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc
     3481 caattaagtg ctgaacgcag cttcgccagc ctgagtttgc gtgaagtggc gcgtgaagcg
     3541 ggcattgctc ccacctcttt ttatcggcat ttccgcgacg tagacgaact gggtctgacc
     3601 atggttgatg agagcggttt aatgctacgc caactcatgc gccaggcgcg tcagcgtatc
     3661 gccaaaggcg ggagtgtgat ccgtacctcg gtctccacat ttatggagtt catcggtaat
     3721 aatcctaacg ccttccggtt attattgcgg gaacgctccg gcacctccgc tgcgtttcgt
     3781 gccgccgttg cgcgtgaaat tcagcacttc attgcggaac ttgcggacta tctggaactc
     3841 gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg aagcaatggt gacaattgtc
     3901 ttcagtgcgg gtgccgaggc gttggacgtc ggcgtcgaac aacgtcggca attagaagag
     3961 cgactggtac tgcaactgcg aatgatttcg aaaggggctt attactggta tcgccgtgaa
     4021 caagagaaaa ccgcaattat tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc
     4081 aagatagagg tacgctgctg ctggcgttgg ttgctggttt atcgattaat ggtactttcg
     4141 cagcgctgtt tagctccatt gtgccatttt ctgtattccc gattatttcc ctggtgctga
     4201 cggtttactg cctgcatcaa cgttatctta atcgcaccat gtcggtaggc ttgccgggtc
     4261 tggcagctgc ctgttttatt ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat
     4321 atccggatat cggctctaac ttcttcccgg cagtactctc cgtcattatg gtgttctgga
     4381 ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga gtaatcggta tcatgccgga
     4441 tgctggcgca tccggcatgg gttttacttc gcggtcagta atacgccgca ctccatatgg
     4501 tgcgtgtagg ggaactgatc aaacagagcc agacgttcga ccttgtgcgt ctggcttaat
     4561 gtttccagat ttttgcataa cgtttccgga ttgcaggaga tatacagaat acgtggatac
     4621 gcctgcacca ttttctcggt ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg
     4681 gtttcgaact gatagctctt taagtcgatc ccttgcaggc ggttaaactc gcgcacacca
     4741 ttcatcgcct gagtaaattc ttctgccgcc atacgaataa tttgtacgtt atcaatatgg
     4801 ttagcggcaa tgttgtattg cgcggcagcg actgacggtt tggcgatttc ggtggctaat
     4861 acccgatcaa aattgcgcgc cagcgctaat gaaaagttac cgttgccgca gtacagctcc
     4921 agtaaatcgc cttttgagcc tttggttacg tccagcgccc attccagcat ctgaatattc
     4981 atcgccgcgt tcggctgggt aaagctgttt tctacctgac gatagatcat ctctttccct
     5041 gcgaccggca gacgctcgtc gatgtaatct tgatccagcg cgattttggt tttcgttgcc
     5101 cgaccaatca gatgcacatt cagattctgc gcgcgcagtg catcgcgcag tgcttccgct
     5161 tcctgacgcc actcatcatc cagcttttta tggtacagca gggaaaccac cgcctgatta
     5221 ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc
     5281 acacccgcaa tcatcgccgt catcaactgg ttgatgagtt cactggcggc ggggaagcta
     5341 tccacgcgga tgcggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca
     5401 tcgtgccaga tgcggaactc cgcgcgcatc cggtaatgac tgaccggcga gcgaaacact
     5461 tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct
     5521 aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa
     5581 ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct
     5641 atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgccggtcc
     5701 tgtgagttaa tagggaatcc agtgcgaatc tagagctgac gcgcagcggt aaggaaaggt
     5761 gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct
     5821 tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg
     5881 cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa
     5941 aaaagcttcg ctgctgacgg cgtgttccgt cacggcattt tccgcttggg cacaggatac
     6001 cagcccggat actctcgtcg ttactgctat ccgttttgaa cagccgcgca gcactgtgct
     6061 tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa
     6121 tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct
     6181 ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ctggtgttaa ttgatggcgt
     6241 ccgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc ctattgcgct
     6301 tgtccagcgt attgaatata tccgtggacc gcgctccgct gtttatggtt ccgatgcaat
     6361 aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg
     6421 gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac tgggggataa
     6481 aacacgggta acgctgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta
     6541 tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta
     6601 tggggcgctg gagcataact ttactgatgc ctggagcggc tttgttcgcg gctatggcta
     6661 tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc tcgatacccg
     6721 taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac tgattaaatc
     6781 acaactcatt accagctata gccatagcaa agattacaac tacgatcccc attatggtcg
     6841 ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt gggcaaacaa
     6901 tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac agactacgac
     6961 gccgggtaca ggttatgttg aggatggata tgatcaacgt aataccggca tctatctgac
     7021 cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagcg acgataactc
     7081 acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca tcgaaggtta
     7141 tcgcttcatt gcttcctacg ggacatctta taaggcacta aatctggggc aactgtatgg
     7201 cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga
     7261 aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgactt
     7321 gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg
     7381 cgtcgaggcg acagccaatt ttgataccgg accactgacg catactgtga gttatgatta
     7441 tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta aacagcaggt
     7501 gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt atcagtattt
     7561 aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg
     7621 tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcacctga cagttcgtgg
     7681 taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg
     7741 acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt
     7801 ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc
     7861 tccattacat ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt
     7921 ttattgttga gcgagtggtg gcaattgtca ccgcggtgca agaacgttat ccccttgcgc
     7981 tggctgtggt cgcttgcaac accgccagta ccgtttcact tcctgcatta cgcgaaaagt
     8041 tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa
     8101 atggcattgt cggattactg gcaacccgcg gaacagttaa acgttcttat actcatgagc
     8161 tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg
     8221 agttggctga agcgaagctg catggcgaag atgtttctct ggatgcacta aaacgtatcc
     8281 tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt tgcacccatt
     8341 tccctctact acaagaagaa ctgttacaag tgctgccaga gggaacccgg ctggtggatt
     8401 ctggcgcagc gattgctcgc cgaacggcct ggttgttaga acatgaagcc ccggatgcaa
     8461 aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat
     8521 tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat
     8581 ttggttgaat gttgcgcggt cggaaaatta ttttaaattt cctcttgtca ggccgggata
     8641 actccctata atacgccacc actgacacgg aacaacggca aacacgccg
//
LOCUS       DAJTOW010000146         8652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTOW010000146 DAJTOW010000000
VERSION     DAJTOW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.146"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(756..1952)
                     /gene="hemY"
                     /locus_tag="OO244_003657"
     CDS             complement(756..1952)
                     /gene="hemY"
                     /locus_tag="OO244_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5665337.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1955..3190)
                     /gene="hemX"
                     /locus_tag="OO244_003658"
     CDS             complement(1955..3190)
                     /gene="hemX"
                     /locus_tag="OO244_003658"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5665338.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            complement(3212..3952)
                     /gene="hemD"
                     /locus_tag="OO244_003659"
     CDS             complement(3212..3952)
                     /gene="hemD"
                     /locus_tag="OO244_003659"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5665339.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3949..4890)
                     /gene="hemC"
                     /locus_tag="OO244_003660"
     CDS             complement(3949..4890)
                     /gene="hemC"
                     /locus_tag="OO244_003660"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5665340.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5277..7823
                     /gene="cyaA"
                     /locus_tag="OO244_003661"
     CDS             5277..7823
                     /gene="cyaA"
                     /locus_tag="OO244_003661"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5665341.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7863..8183)
                     /gene="cyaY"
                     /locus_tag="OO244_003662"
     CDS             complement(7863..8183)
                     /gene="cyaY"
                     /locus_tag="OO244_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5665342.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8231..>8652)
                     /locus_tag="OO244_003663"
     CDS             complement(8231..>8652)
                     /locus_tag="OO244_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5665343.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1967 a         2238 c         2228 g         2219 t
ORIGIN      
        1 tctgctgcgc tggccatgag aggaagagcg gcggcgacag caacaacaag acgtttgggt
       61 gaaaacgaaa attccatgca aaatgctccg gtttcatgtc atcaaaatga tgacgtaatt
      121 aagcattgat aattgagatc cctctccctg acaggatgat tacataaata atagtgacaa
      181 aaataaatta tttatttatc cagaaaatga attggaaaat caggagagcg ttttcaatcc
      241 tacctctggc gcagttgata tgtaaggcag gtttattata tcgcgttgat tattgatgct
      301 gtttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt ttatcattca
      361 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
      421 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
      481 tctctgtggc ataataaacg agtagatgct cattccatct cttatgttcg ccttagtgcc
      541 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
      601 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
      661 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
      721 ggtcccggag gtgctttcct ccgggtgaga aggaactact gtggcgggtt attctgcaac
      781 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
      841 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
      901 aaggcgagcg atgcttcctg ccattcaccg tgcttcatca gtgactgacc caatgtgctc
      961 cacaacagcg ggcgatcacc gacgtttttg atttgctggc gtagcacttt ttccagctgt
     1021 tccgggttat tggttttcag acgcgggatc ggcagcagta ggcgatcgtc atactggcgt
     1081 ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
     1141 tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg gtttttccac
     1201 cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag gccaatccat
     1261 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
     1321 gggatgatat ccagcagaga actccatgcc cctgtgcgga tatacgcctg ttccgccaga
     1381 cgtaatactt caggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     1441 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     1501 tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca
     1561 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc agcgtgatcg
     1621 gcatttttcg ccatcagctt ttcaacttgc tgataatcac cttccgccag tttcagcagc
     1681 gcctgttcgg tctgcttacg cgcacggcga cgcttacgtc cgacaaacca tccacgggtg
     1741 tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag cactaccatc
     1801 gccagaatca atatgatcgc caggcccgtg acgctggttt cgatattgta gttgtcggtc
     1861 tggatcagca cataaccctg atgaccggca atcatcgggc caaccactat ccccgcaatc
     1921 agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     1981 gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca
     2101 ggttacgcac gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg
     2161 gaagatccat cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg
     2221 tggtggcatc atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct
     2281 ggcgataagt ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc
     2341 gaatattttc gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat
     2401 cacgacggcg aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga
     2461 tacgccattc gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat
     2521 cgctgtcatt atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa
     2581 ggatgatgcc gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa
     2641 ttgcccgacg aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac
     2701 ttttcagcaa cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga
     2761 gtttcaccag aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg
     2821 tggcgatctt ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg
     2881 cctgcttaag ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct
     2941 cctgggcttt ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat
     3001 tgacggcctg ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga
     3061 tagccaccgc gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag
     3121 gttgtgacgt ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt
     3181 gttccgtcat tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt
     3241 tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca
     3301 aacgctcact gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg
     3361 ggatcagcga ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct
     3421 cgcgggattg ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat
     3481 aacattcaca aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac
     3541 gaccgccatt gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta
     3601 attgtagcaa gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta
     3661 cggtatgtag cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc
     3721 atttacgatc ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg
     3781 caaacaacag atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc
     3841 gacccggaga aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac
     3901 ggctcactaa ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc
     3961 ctctccgtta tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc
     4021 cagcgaaatc cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat
     4081 ctgcgaaccg tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc
     4141 ggcgtagcta ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc
     4201 tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg
     4261 tgtatcatca aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat
     4321 ctcgggtggt aacgcggcgc gaatgcggga ctccagccct aaacgtttta gtccggctac
     4381 ggcaagaatg atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt
     4441 gccgcgcagg gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg
     4501 taaactggac gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt
     4561 ggacacaaag gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg
     4621 cgggaattca accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag
     4681 gagcgcgact tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt
     4741 atcaagaatc acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg
     4801 gctcgccatc aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg
     4861 tgtggcaatt cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc
     4921 atcctaacat ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa
     4981 accgtaaaat gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga
     5041 atttacagag aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc
     5101 agcaaggtgt taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc
     5161 aggcgcgaaa agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt
     5221 accgttgatg ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt
     5281 acctctatat tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc
     5341 gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt
     5401 tgcactatca ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt
     5461 gcctttacac gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg
     5521 gaatgtcagt acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg
     5581 gcagcacctc gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc
     5641 aatcctggct cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa
     5701 gctgggccgc ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc
     5761 gtcataatga aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc
     5821 tgcttgacga attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata
     5881 tggtgccgtg cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg
     5941 gcgtgctgac gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag
     6001 agtactttgg tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg
     6061 tgctgaaaac actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg
     6121 cgaaagatat caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat
     6181 actgcatgat gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc
     6241 tggatttagt acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg
     6301 cctgtgtagg ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg
     6361 aagctcgtct ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg
     6421 cgcacaacga gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc
     6481 gtcgcaataa ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac
     6541 tgtatgccgc gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac
     6601 ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt
     6661 caggttggta tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc
     6721 tggaatataa ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga
     6781 cctcgcgcac ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg
     6841 agatggtcgc cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg
     6901 cgctctacag cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc
     6961 cgacagctgc gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca
     7021 gctttggcga gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt
     7081 ggaacgaagt gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa
     7141 ctattctcgg caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt
     7201 atagccagca tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta
     7261 ttgaactgcg cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt
     7321 ctggtcaaac ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga
     7381 acgccatcga gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg
     7441 ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca
     7501 tccagttctt tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg
     7561 aaagcaaccg ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg
     7621 acgtcagtcg cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca
     7681 acttcaacct gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc
     7741 cgttccgaac aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc
     7801 tattacagca gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa
     7861 tcttagcgga aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc
     7921 cagaaggttt cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag
     7981 ccgccctgtt tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt
     8041 ttgctgccgt tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg
     8101 ctgtcgccat ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg
     8161 cgatgaaatt cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag
     8221 cggcatgggg tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat
     8281 ccgtcaccgg aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac
     8341 gagttttcgc cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa
     8401 cattgcgatc gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa
     8461 tttgcaagct ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa
     8521 atgagcgatc aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac
     8581 caaatgtcga taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa
     8641 tcagatggaa tt
//
LOCUS       DAJTOW010000147         8390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTOW010000147 DAJTOW010000000
VERSION     DAJTOW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.147"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..2265)
                     /gene="xdhA"
                     /locus_tag="OO244_003664"
     CDS             complement(<1..2265)
                     /gene="xdhA"
                     /locus_tag="OO244_003664"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5665344.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     PLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRL"
     gene            2680..3435
                     /locus_tag="OO244_003665"
     CDS             2680..3435
                     /locus_tag="OO244_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5665345.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3514..3587
                     /locus_tag="OO244_003666"
     tRNA            3514..3587
                     /locus_tag="OO244_003666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3546..3548,aa:Gly,seq:ccc)
     gene            3722..5320
                     /locus_tag="OO244_003667"
     CDS             3722..5320
                     /locus_tag="OO244_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5665346.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQLLPKNRKQRFKAMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSLEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDIRTTEAGAVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLVESLSVKK"
     gene            5363..5971
                     /locus_tag="OO244_003668"
     CDS             5363..5971
                     /locus_tag="OO244_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665347.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
     gene            6902..7648
                     /locus_tag="OO244_003669"
     CDS             6902..7648
                     /locus_tag="OO244_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5665348.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            7946..>8390
                     /locus_tag="OO244_003670"
     CDS             7946..>8390
                     /locus_tag="OO244_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665349.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAA"
BASE COUNT         2183 a         2083 c         2123 g         2001 t
ORIGIN      
        1 taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca tcttcaccgc
       61 gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact tatgtccgta
      121 tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca gatccggcat
      181 ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt tagcatcgat
      241 gatcatctct tcaaatagcg cccagccaat gcccattccc attccgccgt gtacctgacc
      301 ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga tgcggttgat
      361 ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc caaacgccgg
      421 tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc gttcagggtg
      481 gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg gtcgttcaac
      541 cagcacgata tggcctttta tcaggggcaa attcatcgct gactgatgta gcatgactgc
      601 ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg caggcgcggc
      661 aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt cggtatcttg
      721 tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca ccatttgcga
      781 gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt tgatggttcc
      841 atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc aggtgttaga
      901 ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct ggttctggca
      961 ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac actccggcaa
     1021 ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc gggcggcgtt
     1081 gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat caagcataga
     1141 ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag ccgaggggag
     1201 gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa ggtaagcgac
     1261 tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc cggtgttaga
     1321 cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca tttgcccgtc
     1381 aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc taagggaaac
     1441 tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct cttccagtac
     1501 atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta cgcatgacca
     1561 gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct gggtgctgga
     1621 aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc tttccatgtg
     1681 acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg cggcatcgat
     1741 tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc cgccgttatg
     1801 gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg gtaattcttg
     1861 ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc gggccacgac
     1921 gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc gatcggcggt
     1981 atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg gaatatcagg
     2041 cacatcttcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt gttcatcatt
     2101 aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt aacacatgcc
     2161 cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa tggcatcgac
     2221 gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa tcccctcgat
     2281 ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt acgccagaaa
     2341 cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca cagatatgat
     2401 acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc gataaaatca
     2461 atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa gtcaggaact
     2521 tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa tgaaattaac
     2581 ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag aggctggctg
     2641 ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg acgcctgaat
     2701 aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc gggctgttcg
     2761 ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt gaaacggggg
     2821 gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct ggcgcgactg
     2881 aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact gggtggggcg
     2941 aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac cgcatcggtt
     3001 acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg ttgttggtta
     3061 tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg caataaaggg
     3121 attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg aaaggtggtg
     3181 tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca cagtgaagat
     3241 tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca aagcgtgaag
     3301 gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg cctgcatttc
     3361 cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc gcagggcagc
     3421 aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt gctaaggaga
     3481 gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg gtagaacgag
     3541 agcttcccaa gctctatacg agggttcgat tcccttcgcc cgctccatga cacttcttaa
     3601 agtctactaa cttactgaat cctaagggat acggcttaac gccttccgat agttttactc
     3661 actgtaacca acactgtgac taactttgtt gaagtagtga gcagacctaa ggtctcagta
     3721 catgctcaag tcaagaacct acctttatca gcgtaatggt gtcttttaca tccgtcttag
     3781 gatgaagacc actagtcgcc tgaccgcttc gcttccttct cacaaccgtt ataaactagc
     3841 gtcagtatct ttacggacca aggacagacg caccgctatg gctcactcac ggcacatcaa
     3901 gtcagcactt aaagcaatcc atgcagacaa ccctaacgcc tcttatgagg agctacggga
     3961 gcacctgaag accattgtcg agtgggaact tagtgtcagc cgtgatgacc ttaacgaccc
     4021 ggagtcctac cagctttacg ttgaccagta cgacgacatc aagtccaacc ttcgggaagc
     4081 tgtagcgact gagcgtctga ctgtagacca gcatcgctat atcaatgacg ttatcggtgt
     4141 acttaaagcc tgtcaggata gactcaaggg cgataactct ggcctgttgt cttaccttga
     4201 gcctgagact ggcagtctaa gaccgtccgt ctccctatct gtattggcgg aacctgaagt
     4261 ccccgaacct aaagctctga ccctcgcgtc tctcattgag cagtacgagc aggagaacgc
     4321 ccagaactgg aagccagcga ccctaagcga gaaccgagcg tctcactcta cgttaatcga
     4381 gatattcgac tatctggata ttcaggatgt cggcaaggcg acccgtgcag atatgcttag
     4441 ggtccgtgaa gtcctccaac tgttaccgaa gaaccgcaag caacgtttta aggctatgcc
     4501 gctgtctgac ctgctgaacc gggagtctaa gactgactgt ctggacgtcg ttaccatcaa
     4561 caataaatac cttatcaaga tggctgctgt ctttaagtgg gcggtacgca acgacctgat
     4621 agctaagaac ctgacagaag gtcttgaact gaaggtcccg cagcgtaaag cctcagacgc
     4681 tcgtgatgcg ttcagtcttg agcaggtagg gcaactactg gtcgccgcta aagcgtactc
     4741 tcagaagacc tcaggtaagc cataccatta ttacgtcacc gctctggctg ctatcactgg
     4801 ggcgagactt aacgaggtgg cccaacttca ggtcaaagac atcaggacca ctgaggccgg
     4861 ggccgtgtat atccatatca acgaagacga tagcagccta ccgggtaaaa gtattaagaa
     4921 cgctcatagc gaccgctgtg tcccgctggt cgatggcgct tatggttttg tattggctga
     4981 ctttatgtcg cttgtggaag accgtagaaa gactgcgggt gataatgcta tggtattcga
     5041 tggcctcaag ctgatgaaga atggctacgg tgagcaggtg agcaaatggt ttaacagaac
     5101 gttgctgcct aaagtccttg ctgaccgtag cggcttagcc tttcactcat tcaggcacac
     5161 tgttgcgacc cagttaaaac aacatggggt cgagttagcg tatgctcagg cgattatggg
     5221 tcatagctca gggtctataa cttacgaccg ttacgcgaaa gaggtcgagg tggaaacgct
     5281 gaaagaaaaa ttagtggagt cattgtcagt aaaaaaatag acggaaagta atcgcatagg
     5341 gatagcggaa cgtgaggacg taatggtcaa agaattttta gttagcaaag aaaataagcc
     5401 ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa tggcttcaga
     5461 agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt ttgctattga
     5521 ggctatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga cgcagaaaga
     5581 gtgcagaaag acaatgcaca agaatacgtt aaagttgtgc ggtataaaca attatatggg
     5641 gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta ttgctcacgg
     5701 cgattcgtat acatcgagct ttaatttctc ggcggatcat ttggataacc aagacgatat
     5761 tgcaaaaagt gtcttggcgg taaactctga acaatttcgg ggattaactg ttgagcgcct
     5821 tgcaaaatac attgaaatgg caaagcatat tgaccatgag atttctgata acggttacag
     5881 gccttctgaa ggttaccttc cagcagccga gcgcagaagc ctgatgttag cttttggcgt
     5941 cagtggcgtg tcaatgtggc ccagtgcttg agcgttaata caccctcact atagaggaag
     6001 ggactaaact atcactatag ggagagaccc taagtcccca cggcttaata gcagtcacga
     6061 cccatacaca cattcattaa gtctcaggct tccggtagcg gaccgattgg aaaccatccg
     6121 cagacgccta gtgttaaatc accttggcct gagaccattc cttttaacgt aagctatctt
     6181 cccttttgac aggcggggag cagtagacca catccgactt taagttggct ctgtgataat
     6241 cgctgcgcgg ataactgaag aaccaagaca tcattgctga ggtctgccat ccatttgtgg
     6301 acaggcggaa actcaaagag gttagaccag tggtgtctga ggttggagct ttgagtcctg
     6361 accagcaggc ttgaggagac catgagttta tcttgttatg gattgtgtta tcacaattcc
     6421 aataagaaga actttaagtc cagcctccag tcccgccaac ccgaagtaaa attatggtcg
     6481 ctatgatgtg gtctccatgc ttcgggtctt actggaagta ctgaacactt tcgcgtcaag
     6541 ttctccgaag ttcattcctg tttgactaaa ctatcactat agggaccata tctcgcctct
     6601 gacctcctga gagacggcaa gcgagaaacc gtggtccacg ctagcaagct agccaccagc
     6661 ctaaggcaag acagcctctg gtcagcctgt gtgttctttg gtctcgtgac ttaagtctat
     6721 caccacacac gctcggacat gctgtcgaac cttcatctct atggtcatcg cagaccacta
     6781 accccaatta cctcaagtca ttctgtttgc gtcctgttgc ctgctggtct ccctttacgt
     6841 gggggcttga gtaggtcagt ggggtgcgac ttcagggtgc aaacaaaagg agtacacgta
     6901 aatgtcccaa caagaaatca gcatcattaa cctcgaccag cttgtgtcca tgacctcagt
     6961 tgaaattgct gagctgacag gaaaagaaca taaacacgtt ttacgagata ttcgtaatat
     7021 ggtcgaagag cttaacgggg ccaaaactga gcactgctca actttgagca gcgagcttaa
     7081 cgggtccaaa tttgggctcg ttggcgagga agtatacaag gacgcgaaag gtgagagccg
     7141 aacaatgtat cgtctcgacc gtaagcatac tttcattctg gtcgctggct attccgttca
     7201 cctccgcgcc aagtgttacg accatattca gacgttagag cgtcgagtcc ttcaactgga
     7261 agaccagaag aagcgagcag ccattcagtc cgctaaccgc cgaggcgtga cttggggcga
     7321 ctactgcaaa acctatggtc tgcccgctca gaagctaatg accgcacttc tccagcatcg
     7381 tggcctcttt cgtaagaacc caatcagtaa cgagtggtct gtaaatccga agtattccga
     7441 ctgctttcgt atcattaagc cgtcagacca gaagttctca gcaggtgggt ataacttccg
     7501 gttcaacgct aaggggctgg aggtcttcgg taagcctgag atggttgaca agatgcgagg
     7561 catccttatt gcattcactg gcaccgacca gcagaagcaa gagcacctcc tgaagctggc
     7621 acaatccggt aaggtggagg gaatctaaga tgatgattga cccgcgcaca cctgaggggc
     7681 gcatgactct ccgttaccgt ggctaccgga ctgaagtcct cctcagagag cttggtctag
     7741 acccggaaga tgagacccgc cagcaccaat cccgagacga gctaatagcc caactggtcg
     7801 ctatgaaact gccgcttaac cgttagtagc tcacctactc aatacctaat ttaaacacct
     7861 tttgagatga ccgatgcggg gagacccgta ggccttactg tgcctgcgat accgatagtc
     7921 tcaactctca agaggagaat tacctatgat gttcaacaac aacaactgga agctgtcagt
     7981 caccgacatc aacctttatg aaaacactgt gagtctggac ggtcagtcct atccgttgtc
     8041 gctcgctatc aagaccctca ttccgggcta cctgtcaggc ttaccgtcca ccagccgcga
     8101 ggcgatggag ttactggaag ctctggctga agctggcgtg accattggta acttcttcag
     8161 taatgacctg atgaccgcct accaacgccg ccagatgaac aaacgagctg aagctgagcg
     8221 tatcgctaaa gagctagcgt ctcagaaaga gcgtacccgc gaaatgttca tgaccgaaga
     8281 cgaatggcaa aaagagctac agcgccgcga acaggtcaaa gctgaacgcc ggacctatgg
     8341 tgaaaacctc cgcagcgcta ctcattccgc tggtcgctca cgtgccgcta
//
LOCUS       DAJTOW010000148         8092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTOW010000148 DAJTOW010000000
VERSION     DAJTOW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.148"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..673
                     /gene="espL1"
                     /locus_tag="OO244_003671"
                     /pseudo
     CDS             1..673
                     /gene="espL1"
                     /locus_tag="OO244_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            845..952
                     /locus_tag="OO244_003672"
     CDS             845..952
                     /locus_tag="OO244_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665350.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1005..1763)
                     /locus_tag="OO244_003673"
     CDS             complement(1005..1763)
                     /locus_tag="OO244_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5665351.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKTFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2050..2979
                     /gene="pfkB"
                     /locus_tag="OO244_003674"
     CDS             2050..2979
                     /gene="pfkB"
                     /locus_tag="OO244_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5665352.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3080..3370
                     /gene="ghoS"
                     /locus_tag="OO244_003675"
     CDS             3080..3370
                     /gene="ghoS"
                     /locus_tag="OO244_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5665353.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3476..4336
                     /locus_tag="OO244_003676"
     CDS             3476..4336
                     /locus_tag="OO244_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5665354.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4377..4913)
                     /locus_tag="OO244_003677"
     CDS             complement(4377..4913)
                     /locus_tag="OO244_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5665355.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5060..5728
                     /gene="hxpB"
                     /locus_tag="OO244_003678"
     CDS             5060..5728
                     /gene="hxpB"
                     /locus_tag="OO244_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5665356.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LYKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5891..6481
                     /locus_tag="OO244_003679"
     CDS             5891..6481
                     /locus_tag="OO244_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5665357.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6614..8004
                     /gene="tcyP"
                     /locus_tag="OO244_003680"
                     /pseudo
     CDS             6614..8004
                     /gene="tcyP"
                     /locus_tag="OO244_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2099 a         1940 c         1947 g         2106 t
ORIGIN      
        1 ggacatactg acaacgtgaa aatatttatg caagaaattc agtcactggt agataatcac
       61 atcattcatg aagataatct ggttaaatta ctgcaaacta aaagtgctaa cgaaacacct
      121 ggactttata tctccatgtt gtatggattc gatgaaataa tcgatatctt tctgaataca
      181 ttaaccactc caatagcact aagagctttt aaacaaaaaa ctggtgatga gtattttagc
      241 catgaaaata catgatggtg agccaggatt atacgccgca atggaaaata atcacccttt
      301 gtgtgtcaca cggttcctct ctaaaattaa tggcatcgcc tttaaataca agttgagcaa
      361 agctaacatc atggatttat taaaaggagc tacagcacag ggaacccctg cattatacat
      421 cgccatgagc aagggtaatg aagacgtcgt gttatcttat atatcgacgc tgggtgcttt
      481 tgcaaaaaaa cattctttta gtcaacatca gttatttaca ctattggccg ctaaaaatca
      541 tgacaacatg tcagctgttc atatagccat tcatcataag cattataaaa ctgtagaaac
      601 atattatgct gctataaatg tcatcagcca aagcctgagt tttagtgctg atgaaataaa
      661 gacgtattta taaccagcta tttacgatat atatcgatac ataattttat ttttttcata
      721 aaacgcagaa acagtgacca tgtgcacaac gccacccgac ggctgtaata acaaaaaatc
      781 ccttgatgcc tgtccctttt gttacactcc gttatcacgc acaagagata tgcaggacac
      841 tggtatgccg actaaacgct ttgataaaaa acactggaag atggtggtgg tgctactggc
      901 aatctgtggc gctatgttgt tgctacgttg ggcagcaatg atttggggct gagcagtgac
      961 aaacgaagac agccagaccg cataacacgg tccggcagat aattttacat gctgtagccc
     1021 agcgataagg ttgtccgacg gtcggtatgt tccggtgcag actctggagg ttcagagttc
     1081 catgtgacgt tgtaagccac cttcagtcca aaatgttcat taatagcaac atttaatgcg
     1141 ctttcggagt tcagcgttgt gtcttccgcg ccaaagaccg aaacaccctg cgtaaattta
     1201 gcgttgtcag tcaactgcca tgcataagcc ccggaggcat agcccagcgg ctgggtttca
     1261 ctggcattgt cggtatattt gtcgtaacgc acacctggac cgaattcaaa gcggaaactg
     1321 tgtaccgggc cattcaaaaa ctgacgacca taacccgcgg tcaacacatc gcgctctcga
     1381 tagccgttat aacggtctgt cagccagctt gcctgaccaa ataaatagtc ataatcagtt
     1441 aaattaaagc ggctacgccc gcccgccgca tatttttctg aagaacgctc atcattagaa
     1501 gaagtattac tggcgttccc ccacagcgac caggcagtgg tttgtccata ccaggtcatg
     1561 gtggtgtcag ccgtaagtga ggagcttttc gtattgcctg attgtgcaag atatcctgcg
     1621 ttcagattac cttcgaaagt ttttttagcg ctggcaggat cgtccatgac agtaaaaacg
     1681 gaatcatcgg cagctgcatt cagtgacgca aacatgcccc ccgccaacat aacgatggca
     1741 ggaactgtct tcaaaagctt catttatcaa gagtccgtac aacaaaaaaa gagaccatcg
     1801 cggtcccgga aactttctta aggatcaaag attagcgtcc ctggaaaggt aacgaattat
     1861 aaaaaggcgc gaataactta gcaatgtatt cttatttcat tttttgaata agcatgtggc
     1921 gaaaacagat ttttatttat atatatttat ctgcaaaatt ttaaataaag ctctaataaa
     1981 tcatattgtt aatttcttca ctttccgctg attcggtgcc agactgaaat cagcctatag
     2041 gaggaaatga tggtacgtat ctatacgttg acacttgcgc cctctctcga tagcgcaaca
     2101 attaccccgc aaatttatcc cgaaggaaaa ctgcgctgta ccgcaccggt gttcgaaccc
     2161 gggggcggcg gcatcaacgt cgcccgcgcc attgcccatc ttggaggcag tgccacagcg
     2221 atcttcccgg cgggtggcgc gaccggcgaa cacctggttt cactgttggc ggatgaaaat
     2281 gtccccgtcg ctactgtaga agccaaagac tggacccggc agaatttaca cgtacatgtg
     2341 gaagcaagcg gtgaacagta tcgttttgtt atgccaggcg cggcattaaa tgaagatgag
     2401 tttcgccagc ttgaagagca agttctggaa attgaatccg gggccatcct ggtcataagc
     2461 ggaagcctgc cgccaggtgt gaagctggaa aaattaaccc aactgatttc cgctgcgcaa
     2521 aaccaaggga tccgctgcat catcgacagt tctggcgaag cgttaagtgc agcactggcg
     2581 attggtaaca tcgagttggt taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc
     2641 gaactcaccc agccagacga tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag
     2701 gccaaacggg ttgtcgtttc ccttggtcca caaggagcgc tgggtgtcga tagtgaaaac
     2761 tgcattcagg tggtgccacc accggtgaaa agccagagta ccgttggcgc tggtgacagc
     2821 atggtcggcg cgatgacact gaaactggca gaaaatgcct ctcttgaaga gatggtccgt
     2881 tttggcgtag ctgcggggag tgcagccaca ctcaatcagg gaacacgtct gtgctcccat
     2941 gacgatacgc aaaaaattta cgcttacctt tcccgctaac aaaaacattc ccccagcatt
     3001 gggggaatca ccaccaacct gtcggcaacg cgtttctccg actatgctca aaagtcatgt
     3061 gataacaaag gggtgaacta tggccagtgg cgatcttgtc cattatgtca taaccgtcat
     3121 gttgcatgag gatacattga ctgaaattaa cgagttgaat aattacctga ctcgcgacgg
     3181 ttttttgctc accatgacgg atgatgaggg aaatatccat gagctgggga ctaacacttt
     3241 tggacttatc agtacccaaa gtgaagaaga aattagagaa ctggtttcgg ggcttaccca
     3301 aagtgcaacc ggcaaagatc ctgaaatcac catcacgacc tgggaggaat ggaatagcaa
     3361 cagaaaataa atggtttttg ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt
     3421 acaccgcatt cttgcgctaa ccttatgatc tggcagacaa catgggagag acatcatgtg
     3481 gcaggcaatc agtcgtcttt tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa
     3541 tgaactgcct ggcggagaag tccatgccgc atggcatttg cgctatgcag gacatgactt
     3601 tttcgtcaaa tgtgatgaaa gggaactgct tcccggtttt accgccgaag ccgaccaact
     3661 ggagttactg tcgcgtagta aaaccgtcac cgtgcctaag gtttgggcag taggcgctga
     3721 ccgtgactac agttttctgg tgatggatta tctcccacct cgtccgctgg atgcgcatag
     3781 cgcatttatt cttggtcagc aaattgcgcg tttacatcaa tggagtgacc aaccacaatt
     3841 tggcctcgat tttgataact cgctctccac aactccacag cccaacacct ggcaacgtcg
     3901 ctggtcaacg ttttttgctg aacaacggat tggctggcag ttggaactgg cagcagagaa
     3961 agggatcgct ttcggcaata tcgacgccat cgtcgagcat attcagcagc gtctggcctc
     4021 gcatcaaccg cagccttctc tgttgcacgg cgatttatgg tccggcaact gtgcgctggg
     4081 tccggatggc ccgtacattt tcgaccctgc ctgcgactgg ggtgaccgag agtgcgacct
     4141 agcgatgtta ccgctgcata ctgaacagcc gccacaaatc tatgacggct atcagtcagt
     4201 atccccgcta cctgccgatt tccttgaacg tcaaccggtt taccaactct acacgctgtt
     4261 aaatcgtgca agattatttg gcggtcagca tttggttatt gctcagcagt cattggatag
     4321 attattagca gcatgatatg ggttgaggat aatggccgct ccgtgcggcc ttttgattaa
     4381 ataaacccaa gcagagaaaa gaacacataa cctgcagcaa tacaaataac cggcaggata
     4441 tatagtgaga aaaactgcag gaaaatagta tggtgcggaa caacaatacg ggactcaatt
     4501 tgttcacgcg ttagcccctc ttcccctttg gctttttcca gaatgagttg atcttcaaca
     4561 ccttcccgca agaagcgcgc ctggcgactc atccgggcac cggaatcctg caacgccagt
     4621 ccgacaaaaa tcaggatgaa aatcacccag aacataacgt tcacaccacc attaaaatta
     4681 ggggtcgggg agttatacca gaacagattc aaaaaaggcg tattcgcctg catcatatcg
     4741 atcatgacat gagcaaagtc gagcatgacc gcattaatgc cttcctggtt ttcctgccgg
     4801 gtattcataa atttcagcaa agaaatcaat gtagagatca atgcaggtat aaaaatcacc
     4861 caacccaaaa tccttttcaa aacagcaatg cgtccagctt gttgatacgt catgagttct
     4921 ccttgattaa gacgcgtcgt ttcacttagt ttacctgtag atatctgttt tcgcccattc
     4981 tttaaaggcg atatgatagg cgcttaatca taagcacggc ttaatacctt acacataatg
     5041 ctctaaagga gaggttgtaa tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat
     5101 ggatggatta cttatcgact cagaaccttt atgggatcga gccgaactgg atgtgatggc
     5161 aagcctgggg gtagatatct cccgtcgtaa cgagctgccg gacaccttag gtttacgcat
     5221 cgatatggtg gtcgatcttt ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga
     5281 agtagtagaa cgggttattg cccgtgccat ttcactggtt gaagagacac gtccattatt
     5341 accaggcgtg cgcgaagctg ttgcgttata caaagaacaa ggtttattgg tgggactggc
     5401 ctccgcgtca ccactacata tgctggaaaa agtgttgacc atgtttgact tacgcgacag
     5461 tttcgatgcc ctcgcctcgg ctgaaaaact gccttacagc aagccgcatc cgcaagtata
     5521 tctcgactgc gcagcaaaac tgggcgttga cccactgacc tgcgtggcgc tggaagactc
     5581 ggtaaatggc atgatcgctt ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc
     5641 agaagcgcaa aatgatccac gttttgtatt agcaaacgtc aaactctcat cgctgacaga
     5701 actcaccgca aaagaccttc tcggttaatg accaggggca gtgatcacgc tgcccctggt
     5761 tctttatctg aatttgcatt caactgacgg attaatcgtc aatttaagag aaagagttac
     5821 accgtcacca cttccgtgca ctgtataaaa atcctatact gtacgtatcg acagtttagt
     5881 gagttttatc atgacggcgg aaggtcacct tctcttttct attgcttgtg cggtatttgc
     5941 caaaaatgcc gagctgacgc ccgtgctggc acagggtgac tggtggcata ttgtcccttc
     6001 cgcaatcctg acgtgtttgt taccggacat cgatcaccca aagtcgtttc ttgggcagcg
     6061 attaaaatgg atatcaaaac cgatcgcccg cgcttttggg catcgtggtt ttacccacag
     6121 tctgctggcg gtatttgcgc tgctggcaac cttttacctt aaggttccgg aaggctggtt
     6181 cattccggct gatgcgctac aaggaatggt gctgggttat ttgagccaca tacttgccga
     6241 tatgctgaca cccgccggtg ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc
     6301 tatcctggtt ccccaaaagg gcaaccaact ggaacgtttt atctgcatgg cattatttgt
     6361 ctggtcggta tggatgcccc attcattacc cgagaacagc gctgttcgtt ggtcatcgca
     6421 aatgatcaat accttgcaga tccagtttca tcggcttatt aagcatcagg ttgaatacta
     6481 aaaaggcaaa aatcaccttt ctggaataag caattccatt tgaatataag agccagctca
     6541 cagttctgtt aatcttgcgc caacactatg actgctacgc agtgatagaa ataataagat
     6601 caggagaacg gggatgaact ttccattaat tgcgaacatc gtggtgttcg ttgtactgct
     6661 gtttgcgctg gctcagaccc gccataaaca gtggagtctg gcgaaaaaag tgctggtggg
     6721 tctggtgatg ggtgtggttt ttggccttgc cctgcatacc atttatggtt ctgacagcca
     6781 ggtacttaaa gattctgtac agtggtttaa catcgttggt aacggctatg ttcaactgct
     6841 gcaaatgatc gttatgccgt tagtcttcgc ctctattctg agcgcggttg cccgtctgca
     6901 taacgcatct cagttaggca aaatcagttt tctgaccatc ggtacgcttt tgtttaccac
     6961 gctgattgcg gcgctggtcg gtgtgctggt caccaacctg tttggtttga cggctgaagg
     7021 tctggttcag ggtggtgcag aaactgcacg tctgaacgcc attgaaagta actatgttgg
     7081 taaagtctct gatcagagcg ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt
     7141 tgccgatctt accggagcca atccgacgtc aattatcagc gtggtaattt ttgccgcatt
     7201 cctcggcgta gctgcattga aactgctgaa ggatgatgcg ccgaaagggg aacgcgtctt
     7261 aaccgctatc gataccctac aaagctgggt gatgaaactg gttcgccttg tcatgcagtt
     7321 gaccccttac ggcgttctgg ctctaatgac caaagtggtt gcaggttcta acttgcaaga
     7381 catcatcaaa ctgggaagtt tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc
     7441 tgtgcatggc attctgctgg gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt
     7501 atggcctgtg ctgacgtttg cctttaccag ccgttccagt gctgcgtcta tcccactgaa
     7561 tgtggaagca caaacgcgtc gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc
     7621 tttcggtgca accattggtc agaacggctg cgccggtttg tatccggcaa tgctgcggtg
     7681 atggttgcgc ctacggttgg cattaacccg ctggacccga tgtggattgc gacgctggtc
     7741 ggtattgtta ccgttagttc cgcaggcgtt gccggtgtcg gtggtggtgc aactttcgcc
     7801 gcactgattg tgctgcctgc gatgggcctg ccagtaaccc tggtggcgct gttaatctcc
     7861 gttgaaccgc ttatcgacat gggccgtacg gcgctaaacg ttagtggctc gatgacagct
     7921 ggcacgctga ccagccagtg gctgaagcaa accgataaag ccattctgga tagcgaagac
     7981 gacgccgaac tggcacaccg ttaattctta tgctggcaac ggtccgtttt gtataggggc
     8041 cgttgcttta ctttttaaat gttccatcgc ggcgatgcat actgactcat at
//
LOCUS       DAJTOW010000149         8016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTOW010000149 DAJTOW010000000
VERSION     DAJTOW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.149"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..1333
                     /gene="yjeM"
                     /locus_tag="OO244_003681"
                     /pseudo
     CDS             1..1333
                     /gene="yjeM"
                     /locus_tag="OO244_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1484..4795)
                     /gene="mscM"
                     /locus_tag="OO244_003682"
     CDS             complement(1484..4795)
                     /gene="mscM"
                     /locus_tag="OO244_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5665358.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4817..5785)
                     /gene="psd"
                     /locus_tag="OO244_003683"
     CDS             complement(4817..5785)
                     /gene="psd"
                     /locus_tag="OO244_003683"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5665359.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5882..6934)
                     /gene="rsgA"
                     /locus_tag="OO244_003684"
     CDS             complement(5882..6934)
                     /gene="rsgA"
                     /locus_tag="OO244_003684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5665360.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7029..7574
                     /gene="orn"
                     /locus_tag="OO244_003685"
     CDS             7029..7574
                     /gene="orn"
                     /locus_tag="OO244_003685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5665361.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7785..7860
                     /locus_tag="OO244_003686"
     tRNA            7785..7860
                     /locus_tag="OO244_003686"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7818..7820,aa:Gly,seq:gcc)
BASE COUNT         1830 a         2073 c         2170 g         1943 t
ORIGIN      
        1 ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggtggt atctattcct
       61 ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg tggttttcct
      121 cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc tcaacattcc
      181 tctatggtag tgacatgacc cagcactggc gtattgccgg actggagcct acgcaggtgg
      241 ttggtctgct ggcagtagca tggatgattc tggtcaccgt cgttgcttca aaggggatta
      301 ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg aatttagtat
      361 tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg caggatatta
      421 atttccttgc atcaccgaac ccgggttatc agtccggtct ggcaatgcta tcgtttgtgg
      481 tatttgctat ttttgcctat ggcggaattg aagcggttgg tggtctggtc gataaaacgg
      541 aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt atttcaatcg
      601 gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag gtattaagta
      661 atggttccgt taaccttggc aatattacct atgtgctgat gaagagcctc ggggtgatgc
      721 tgggtaacgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta tggtttgcgc
      781 gtattaccgg actttcgatg ttcctcgctt ataccggtgc gtttgctatt caccgctgaa
      841 agccatcatc caggggacgc cgaaagcgtt gtggccggaa ccgatgacgc gcctgaatgc
      901 gatggggatg ccgtctatcg ccatgtggat gcagtgcggg ttggttactg tcttcattct
      961 gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt aacaagctga cgctgatggc
     1021 gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg gctttcccgt tttttaaagc
     1081 acgtcaggat ctcgacagac catttgtgat tttcaaaacg cgtatgtcgg caatgattgc
     1141 gacggtggtt gtcgttctgg tggtgacatt tgcgaacgtc ttcaccatta ttcagcctgt
     1201 ggttgaagcc ggagactggg acagcacatt gtggatgatt ggcggccctg tcttcttctc
     1261 gctgttagcg atggcgattt accagaacta ttgcagtcgc atggcgaata aacctgagtt
     1321 agctctcgac tgatcatatg aaatcctgca actcatcgtc tctccggcga tgagttgtca
     1381 ggaagtaatg cgtatggatg atactctcca gggccggtga taactgaaga tgatgttaag
     1441 ctgctaaatg agccacgttg acatcatttg gcagcttcat gcattacaaa cttcccgcct
     1501 gacgaccttt gcccgcagac gtcagcgttc tccccgtttg tttaccgttg aggctttcca
     1561 gacgcatctg gaagggcggg aatggcatat cgataccgtg ggcgtggaag cctgccagaa
     1621 tcagctggtg gatctcatgg cgtagcggca tacggtgacc catctcagcg gcgtaaatac
     1681 gcagctcgaa aatctgaatc ccctgttgca gatccaccag gaagacttcc ggtgccgggt
     1741 tgtcgatcac cagcgagcag cgacgcgctg cggtgagcag gatttccgtc acttcttcac
     1801 tgttggcatc ggcaggggcc ggaatcgtca acactacgcg cgtgaccgag tcagagagcg
     1861 accagttgat aaactgctcg gtaataaacg ccttgttcgg cacgattatc tctttacggt
     1921 cccagtcgct gatggtggtg gcgcgggtgt taatttttgt cacgctacca gtaagatcgc
     1981 ggatcgttac cgtatcgcca atgcgaatcg gtttttcgaa caggatgatc aggccagaga
     2041 taaagttggc gaaaatttcc tgcaaaccaa aaccgagacc aacaccgagc gcggcaacca
     2101 gccactgcaa tttcgaccac tcaataccaa tcattgagaa gccgaccagc ccgccaatca
     2161 gcatcagcag atattttgtg atggtggtga tggcgtaacc cgtacccggc gttaaatcca
     2221 ggtgctgcaa aatcgccagt tccagcagcg cgggcaggtt gcgcaccagc tgcgtggtga
     2281 tgataaatac cagaatggca atcagcaccg caccgagggt aattggctcc agactttcta
     2341 cgccctgtac cgtggaggtg acatcccaca gcgaaatatt ttcgaggaag ccgaaagcgg
     2401 aatggatttc tgaccacagc acaatgaccg aaagcagggc gatcaacatc aaaattgagc
     2461 gcaccagccg caaggattgc gcactgatgg catcgagatc gacttcgctt tcatcgactt
     2521 caattgctcc ttccgggcta ctgtgatgat gcgcttcctc ttcgccacgc gcacgttgcg
     2581 ctaacatctc tgcccggcga tgcttcgccc ggtcaaacgc cagcctgcga cgctggatca
     2641 gcatccagcg gcggataacg tgataaacca ccagtagcag gaaccagatg gcaaccgagg
     2701 tttcaagcct cgctaacagt gcctgcgccg ttgccagata acccaccgcc gacgccagaa
     2761 tggcaaccag tggtgcgcca atcatcatgt tccacagcat atggttggta atgttgtcgc
     2821 cgctgccttc tttgttgaga tacaacggaa taccggcttt tttcaggctt agggtgacca
     2881 ccgccagcgc accgcaaatg aggataaagc aaagccgtcc cagcgaaccg gaaaattcgc
     2941 ggtcgtcgag gttatcgaac atcatcagcg ccataatcag cccgatgctc atcaggtagt
     3001 agcgcatccc acgggaaaca cgttcgcgcg gccagccaaa atgagcgata aacaagccgt
     3061 tcgggcgggc aaaggtggcg caaatcatca ctacccacag cagcggcacg gtggccgtta
     3121 caccatcgcc aatcgcgacc gccagcggat aaggccacgc ctcgcgcaag ccgtaaccca
     3181 gcgtcatcca cagcaccggt aacggtgacg cgacgagaat cgaccagaaa agagtgcgca
     3241 acgtcagcca gaagtgatcc tgagtcactt tgccgacttt cgccgccgaa cgttcaagaa
     3301 aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac cagaatcaac gcgccaaaca
     3361 gcggcaaaat tgtctctttg ctggtcagca tcatcacact ggctttgccc aactgactga
     3421 aggtgtccag cgaaatgaga cgacgcagat cctgggcgat ttccagcggc caggcgatgg
     3481 tcatcgggcg cacgtcagag gcccagaaca gatagcggtg cgttgcttcg ttcacctctt
     3541 tcagcgcatc ctccagttgc ctgttggaga ctttcagctt ggtcagttcc aatagtagcg
     3601 tgtcgccacc ctgcaacaat gagttcagca actcacgctg agtgcgcagt tgtgcttcca
     3661 gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc ctgatggatt tgccgtagca
     3721 gcggctgttt attaagtaaa tcctcataac gtaaccgttg cacacgcaac tgcgccattt
     3781 cggtatcaag ctgttgtggt tttggcattt ctggtagccg tgccacctgc gcccgcagcg
     3841 cttcgccgag caggttggac gatcccagcc attgcgactg ttcacgcagc gtattcaacg
     3901 cctgccggac ctgtaacgtc tggctggcag cctgacgctg ttgcgaggca acgagctcca
     3961 tccgctgcgc ctgttgattc agcgctgccg atagttcgcg gttaattttg aattgcgcga
     4021 cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa ttcggtactt tccagcgccc
     4081 gctccgcctc aagctgacgt tggctattca actgattacg taaggcctgc aaatacgcat
     4141 ccagttgctg gctctctttt tccgccagct ctgagcgtaa gcgcgctaat tcctggcggt
     4201 tattggcaga cagctgcgcc agctccagtt catcaacgag cgccttaaga cgtgcagagt
     4261 cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt attgccggta agcgttccca
     4321 ggcggcgctc gatctcattt aactgacggc gggcgtcggt ttgctgttgc ggcagttgat
     4381 tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg ctgggcctga cggcttttat
     4441 ccagcaactg gctgctgacc tggagaattt cctgattcag cgcgtcggta gacattcccg
     4501 gcgacacgct gcgcggctcg tcacgaatgt tgtttaattg tgcgcgcaga gtagcggaga
     4561 gtttcggata attatcgata acttgctgat attgtttgat gcgctcaagg gaaccttttc
     4621 gttcctcaag cgcatttaag gcagactgga gcgcctctac gacttccggc tgtgcgggtt
     4681 tcgccgcttt tgcctgctcc agttcctgag tgatttgttt gctatcgggg gccgtcgcgg
     4741 cgtacgcccc ccaactgagg caccaggcca tcagaaaagt gataatcagg cgcacgtcag
     4801 cgtttccttt gatggattag acctggtctt ttttatcgtc aaccaatggg ctggcgtcgt
     4861 gttctgcttc gatctcttca gcaggaagtg gggcgggttc agcgtctggc gtaacaaagg
     4921 tttcggtaga tactgccagc ggctggccaa ttttcgtgac agacaggctt tccagttgct
     4981 caaccagatt cactttaccc ggtgcaaaca ggttgataac ggtggaaccg agtttaaagc
     5041 gacccatttc ctggcctttc agcagtgcca cagaaccgtc gttttccccg gcaggccagg
     5101 tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt gcccgcccag accgtctcaa
     5161 tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat tgggccaaat tcggtatcga
     5221 aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg cacgttctga gcggtgagat
     5281 ggttaacgga aaagagatcg cccggcacgt agatcatctc acgcagaata ccgttgcacg
     5341 gcatgtgtac gcgatggtag tcacgcgggg agaggtaagt ggtcacaaac gtaccgttgc
     5401 ggaacaggtc cgccatcaga tagttgcctg ccagcagggc ttcgaggctg tagttgtggc
     5461 ctttggcttg caggatttta tcttcttcga ttttacccaa ctggctgata acgccatcgg
     5521 caggcatgac cagtacattc ggatcggtat cgattgggcg tacttcgtca cgcagcggac
     5581 ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc cggcttttgc gcctctttca
     5641 tgtcgacctt gtagtattta acgaacagat cgataaccag ttttgtcagc catcctgccc
     5701 gcttgcttgc gccccaaccc gccaggcgag taagccatag tttcggcaga atgtactgta
     5761 gcgaaagttt aaatgaattt aacaaggtag cctccaggcc attgttttgt cgttcctgat
     5821 ccggccaaca tgccggatcc tgaaaaaaag gggacgattc taacgacggt tagcttaatt
     5881 gtcagtcatc cgtatcagaa aagtttttac gcgtttttac ctgcgccatg ctttccagaa
     5941 tacggtgata gttttcgaaa cgggtttccg cgattttccc ttcctcaacc gcttcccgga
     6001 tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata tttacacaga cctaaatagt
     6061 catggaattc gacaaagccc tgagtgattt gttccggctc caggtgccag aggccgaact
     6121 cacgcacacc aggggaatca atcacatcac cgccgtgcgg gaagtgatac agccgagcgg
     6181 cggtcgtggt gtgctgaccg agccccgagt tgtcagagac atcgttggtc aggatctctt
     6241 tttgcagccc cagcaacgcg ttcagcaggc tggatttacc gacgccagac tgcccggcaa
     6301 aaatgctgat gcgcccggtc aacgcctctt ccagcggttt cagcccatcc tgagtatggc
     6361 tggaaaccat caatacgcga taaccgatgt tgcggtagat atccatctgc tcgttgacga
     6421 acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag cacgataatc ggctcaatct
     6481 gcaaggtttc gcaggccacc aggtaacggt cgataatatt gagcgacagc tccggcaaaa
     6541 tggcggagac aatgacaatc tggtcgatgt tggcggctat gggtttcacg ccgtcgtaga
     6601 aatccggacg cgttaacacc gaggtgcgct catgcaccgc ttccacgatc cctttgacat
     6661 ttacgccttc cgccgccggt ttacccggac gccagactac gcggtcgccg gttaccagcg
     6721 aacggatggt acggcgaata ttgcagcggt gaacgtcgcc atcggcggat tccacatcag
     6781 cgtgcatacc aaagcggctg atgacgatac cttcatcagg ctcgccaaac agattgtcgt
     6841 cgtagtcggg cttctcctta gacgttttaa gacgacgctg gtgattggcg ttcacgcggc
     6901 gctgctggcc tttggagagt ttatttttac tcaatcgtac tgactcctgg tcgcccctga
     6961 tgggcaaaac atctatgata cacgcaattg tggatcaata tagtcactgt gaatgggtgg
     7021 aaaatagcat gagtgccaat gaaaacaacc tgatttggat cgatcttgag atgaccggtc
     7081 tggatcccga gcgcgatcgc attattgaga ttgccacgct ggtgaccgat gccaatctga
     7141 atattctggc agaagggccg accattgcag tacatcagtc cgatgaacag ctggcgctga
     7201 tggatgactg gaacgtgcgc acccataccg ccagcgggct ggtagagcgc gtgaaagcga
     7261 gcacgatggg cgatcgggaa gctgaactgg caacgctcga attcttaaaa cagtgggtgc
     7321 ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca ggaccgtcgt ttcctgttta
     7381 aatacatgcc ggagctggaa gtctacttcc actaccgtta tctcgatgtc agcaccctga
     7441 aagagctggc gcgccgctgg aagccggaaa ttctggatgg ttttaccaag caggggacgc
     7501 atcaggcgat ggatgatatc cgtgaatcgg tggcggagct ggcttactac cgcgagcatt
     7561 ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg gaatccactt gcggcggcgt
     7621 gattttgacg ctaaatcgaa cgtcttgctg ataatttcag cacttgagat aaaaacgcaa
     7681 aaaaaacttt tttggggggt tgcagaggga aagatttctc gtataatgcg cctcccgtaa
     7741 cgacgcagaa atgcgaaaat tacgaaagca aaattaagta gtacgcggga atagctcagt
     7801 tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc gagtctcgtt tcccgctcca
     7861 aaatttgaaa agtgctgcaa agcacagacc acccaatcgc agtatttcga attgcgacaa
     7921 ggcggcaact gaacgaatcc ttgggaactt acaaaagtat gtgactaagg tgagtgaagg
     7981 cagccaaggc agtagcggtt cgaaagacga agatta
//
LOCUS       DAJTOW010000150         8000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTOW010000150 DAJTOW010000000
VERSION     DAJTOW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.150"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..81
                     /locus_tag="OO244_003687"
     CDS             <1..81
                     /locus_tag="OO244_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5665362.1"
                     /translation="KGANQCARAGDKLPDLLDEYIRTTTE"
     gene            53..1963
                     /locus_tag="OO244_003688"
     CDS             53..1963
                     /locus_tag="OO244_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5665363.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            1964..2167
                     /locus_tag="OO244_003689"
     CDS             1964..2167
                     /locus_tag="OO244_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665364.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2164..3757
                     /locus_tag="OO244_003690"
                     /pseudo
     CDS             2164..3757
                     /locus_tag="OO244_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3747..5252
                     /locus_tag="OO244_003691"
     CDS             3747..5252
                     /locus_tag="OO244_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5665365.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5289..5636
                     /locus_tag="OO244_003692"
     CDS             5289..5636
                     /locus_tag="OO244_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5665366.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5694..6722
                     /locus_tag="OO244_003693"
     CDS             5694..6722
                     /locus_tag="OO244_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5665367.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6774..7160
                     /locus_tag="OO244_003694"
     CDS             6774..7160
                     /locus_tag="OO244_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5665368.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7172..7549
                     /locus_tag="OO244_003695"
     CDS             7172..7549
                     /locus_tag="OO244_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665369.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7536..>8000
                     /locus_tag="OO244_003696"
     CDS             7536..>8000
                     /locus_tag="OO244_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665370.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1844 a         1880 c         2495 g         1781 t
ORIGIN      
        1 aagggagcga atcagtgcgc gcgtgcgggg gataaattac cggatttact cgatgaatat
       61 atcagaacaa caactgagta atatgatggt ggcagtcact atcgcactgc agccactggt
      121 cagggtattg ccggtgactg ctgtagaatg ggccgatcaa aattattacc tgcctaaaga
      181 atcgtcatac ggtgaggggg aatggaaaac gctgccgttt caggtcgcca tcatgaactg
      241 catgggcaat gacgagattc gtacagttaa cctgattaaa tctgcccgtg ttggttatac
      301 aaaaatggtg cttggtgtga tcgggtattt tattgagcac aaatcccgca acactcttct
      361 ttttcagccg acggactcag ctgctgaaga ttttatgaag tctcacgtgg aagcaacaat
      421 tcgtgatgtt ccttgcctga aaaaactttc tccctggctg ggccggaaac accgggataa
      481 tacgctcaca ctgaagcgtt tttcctctgg tgtggggttc tggtgtttgg gtggtgctgc
      541 tgccaaaaac taccgtgaaa aatctgtcga tgtggtctgc tatgacgaac tctcctcgtt
      601 tgaaccggat gtggaaaaag aaggttcgcc aacactgctt ggcgataaac gtattgaagg
      661 ttcggtatgg ccaaaatcca tacgcggttc aacgccaaaa attaaaggct cctgtcagat
      721 tgaaaaagct gcgaatgaat ctgcgcattt catgcggttt tatgtccctt gccctcattg
      781 cggggaggag cagtatctga agtttggcga tgatgcgacg tcgtttggcc tgaaatggga
      841 gaagggtaaa ccggaaacgg tgtattacct gtgtgaacat aatggctgcg tgatccgtca
      901 gtctgaactt gatcagaccg gggggcgctg gatttgtgac aataccggga tgtggacgtg
      961 tgacggtctg acgtttttca gtgcttcggg taatgaaatt ccgccgccgc gctccatcac
     1021 tttccatata tggacggcgt acagtccgtt caccacctgg gtacagatag tctgtgactg
     1081 gctggatgca ctgaaagatc ccaacggcgt gaaaactttt gtgaacacca cgctgggcga
     1141 gacctgggaa gaggccgtgg gcgaaaaact cgatcaccag gtactgatgg ataaggttgt
     1201 gccttacacg gcaacggtgc ctgtccgggt ggtttatctg acggcgggca ttgactcgca
     1261 gcgaaaccgt tttgagatgt atgtctgggg gtgggctccg ggagaggaag cctttctggt
     1321 ggataaaatc atcatcatgg gacgtcctga tgaggaagag acgctgttac gtgtggatgc
     1381 ggcgatcaat aaaaaatacc gccatgcaga cggtaccgaa atgaccattt cccgtgtctg
     1441 ctgggatatc ggggggatcg atggcgaaat cgtgtatcag aggtcaaaaa aacacggtgt
     1501 tttccgggta ctgccggtaa aaggtgcctc tgtctatggc aagccggtga tcaccatgcc
     1561 gaaaacccgc aatcagcggg gtgtgtatct gtgtgaggtg gggacggata ccgcaaaaga
     1621 aattctctat gcccgaatga aggccgatcc cacccctgtg aatgaggcca catcgtatgc
     1681 catccgcttt cctgataatc cggagatttt ttcgcagaca gaggcacaac aactggtggc
     1741 ggaggaactg gtggaaaagt gggaaaaagg aaagatgcgt ctgttgtggg ataacaaaaa
     1801 gcggcgtaac gaagcgctgg actgcctggt gtatgcctat gcggcattac gtgtgtccgt
     1861 gcaacgctgg caacttgatc tggctgtgca gttgcatccc gcaaggatgc aactgcacaa
     1921 ccgacccttg aagaactggc agcgaagctg tccggaggag tgaatggtta cagtcgctga
     1981 actgcaggcg ctgcgtcagg cgcgccttga tttattaacc ggtaaacggg ttgtgtcggt
     2041 acagaaagac ggtcgcagga tcgaatatac ggcagcgtcc ctgaatgagc ttaaccgtgc
     2101 gatcaacgat gcggagtcgg ttctgggtac gacccgacgc cgccgaccgc tgggagtgag
     2161 gttatgaaac gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc
     2221 agctacaacg gaggcggcgc aggatttggc gggcaaatgg cagagtggtt accaccggcg
     2281 cagagtgccg atgcggccct gttgcctgca ttgcgtctgg ggaatgcccg ggcagatgat
     2341 ctggtgcgca ataacggtat cgcggctaat gcggtggccc tgcataagga tcatatcgtc
     2401 gggcacatgt ttcttatcag ctaccgtctg aactggcgtt ggctggggat gcgggagacc
     2461 acggcaaaaa gttttgtcgg ggaggtggaa gcagcctggt cggaatacgc agaaggaatg
     2521 tttggcgaga tcgacgtgga agggaaacgc acgtttacgg aatttatccg tgaaggggtg
     2581 ggcgtgcatg cctttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg
     2641 cagttattcc gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac
     2701 ggtatgggga accactttct gcgggccggg gttgaggtcg atcggtatgg gcgggcgatt
     2761 gcgtaccata tctgtgagga tgattttccg ttctctggtg ccggacgctg ggagcggatc
     2821 ccgcgtgagc ttcccaccgg gcgtccggcc atgctgcata tttttgagcc ggtggaggac
     2881 gggcagaccc gtggggctaa tcagttttac agcgtaatgg agcggatgaa gatgctggac
     2941 tccctgcagg ccacgcagct tcagtcggcc attgtgaagg cgatgtatgc agccacaatc
     3001 gaaagtgaac ttgataccga aaaggcattt gaatatatcg cgggggcacc gcaaggtcag
     3061 caggataatc cgcttattaa tattctggag aagttcaccc gctggtatga cacgaataac
     3121 gtgacgctgg gcggtgttaa aattccgcac cttttttcct ggcgatgcgc tgaacctgca
     3181 gacagcgcag gattcagaca atggattttc ggcgcttgaa caggcgctgc tgcggtatat
     3241 tgcagccggt cttggcgttt cctacgaaca gttgtcccgt gattactcga aggtcagtta
     3301 ctcaagtgcc cgcgcctccg ccaatgagtc gtggcgctat tttatggggc ggcgaaaatt
     3361 tattgcggcc cggctggcca cgcagatgtt ttcctgctgg ctggaagagg cgcttcttcg
     3421 ggggattatt cgtccgccac gggcgcgttt tgatttttac caggcgcgtt cagcctggtc
     3481 acgggcagag tggattagct ccggaagaat ggccattgac gggctcaagg aggtccagga
     3541 atcggtgatg cgcattgagg ccggactgag cacgtatgag aaagaactgg cactgatggg
     3601 cgaagattat caggacattt tccgccagca ggtcagggaa tccgcagagc gcgaaaaagc
     3661 cggactctca cgtccggtgt ggatagccca ggcgtatcag cagcagatag cggagagtcg
     3721 caggccggaa gaggagacaa catcacgtga gacgtaatct ttcacatatt atcgctgcgg
     3781 cattcaatga gccgctgctt ctggagcccg cctatgcgcg ggttttcttt tgcgcgctcg
     3841 ggcgtgagat gggcgcagca agcctttcgg taccgcaaca gcaggtacag cttgacgctc
     3901 ccgggatgct ggctgaaacg gacgagtaca tggccggagg caaacgacct gcccgtgttt
     3961 accgggtggt gaacggtatt gctgtactgc cggtgaccgg cacgctggta caccggctgg
     4021 gtggcatgcg gccattttcc ggaatgactg gctatgacgg cattgtcgcc tgtcttcagc
     4081 aggcaatggc ggacactcag gtacggggcg tactgctgga cattgacagt tctggcgggc
     4141 aggccaccgg cgcgtttgac tgcgctgaca tgatttaccg tctccggcag ctgaagcctg
     4201 tctgggcact gtgcaatgac acggcctgtt cagccgccat gttactggca tcggcctgct
     4261 cccgacggct ggttacccag acatcccgta tcggctccat tggggtgatg atgggccatg
     4321 tcagctatgc cggtcatctg gcacaggccg gtgtggatat cacgctgatt tacgccggat
     4381 cgcacaaggt ggatggcaat cagtttgaat ccctgccagc aaaggttcgc caggacatgc
     4441 agcagcgcat tgatgcggcg cgccggatgt ttgctgaaaa agtggcgatg tataccggtt
     4501 tatctgtgga tgcggttatg ggaacagagg ccgccgtttt tgaaggtcag tccggtattg
     4561 aggccgaact ggcggatgaa ttaatcaatg cgtcggacgc catcagcgtg atggctgcga
     4621 cgctgaacac acacgataca ggaggcacta tgccgcaatt aactgcaacg gaggctgccg
     4681 cgcaggagaa ccagcgagtg atggggatcc tgacgtgtca ggaagcgaaa ggacgtgaac
     4741 aactggccac gatgctggca gggcaacagg gtatgagcgt tgagcaggcc cgggcgattc
     4801 tggccgtggc ggcaccgcaa caatctggtg tatctgcgca gagtgaagcc gatcgcatta
     4861 tggcatgtga agaggcgaaa ggacgtgaac aactggcggc aacgctggcg gcgatgccgg
     4921 agatgacggt ggaaaaagcc cgtccgattc tggctgcttc accgcaggcg aatgccggac
     4981 cctcacttcg tgatcagatt atggccctgg atgaggcaaa aggggcagag gcgcaggctg
     5041 aaaaactggc ggcctgtccg gggatgaccg tggagaatgc ccgggctgta ctggcggcag
     5101 cctcaggcag ggcagaaccg gtttctgcat ccacaaccgc catgtttgaa catttcatgg
     5161 cgaaccattc accggcagcg gtacagggtg gcgtgccaca gacgtcagca gacggtgatg
     5221 cggacgtgaa aatgctcatg gccatgccat gaagtcagtg ctgaccatca atatgaggtt
     5281 tttacaatat ggtaacgaaa accatcactg aacagcgtgc ggaagtacgt atttttgccg
     5341 gtaatgatcc ggcccatacc gccacaggca gcagcgggat ttcctcggca acaccggctc
     5401 tgacgcccct gatgctggat gaggataccg ggaaactggt ggtctgggac ggacagaagg
     5461 caggccatgt ggttggcata ctggtactgc cgcttgaagg tacagagacg gtgctgacgt
     5521 attacaagtc ggggaccttt gcgacggagg ccatccactg gcctgagaga gtggatacac
     5581 acaaaaaggc gaatgccttt gccggcagtg ccctgagtca cgcagcgctg ccgtaacatt
     5641 ttatcaggtc actgcggtag cctgactgat ttctgaatga aaggaactga tttatgggat
     5701 tgtttacgac ccgccagtta ctcggttata ccgaacaaaa agttaaattt cgtgcgctgt
     5761 ttctggagct gtttttccgc cgtacggtga atttccatac cgaagaggta atgctggaca
     5821 aaattaccgg aaaaacgccg gtggcggcct atgtctcccc ggttgttgaa ggaaaggtgt
     5881 tgcgtcaccg tggcggtgaa actcgtgtac tgcgcccggg ctacgttaag ccgaaacacg
     5941 aatttaatta ccagcaggcg gtggagcgtc ttcccggtga agatccggcc cagctgaacg
     6001 gcccggctta ccgtcgcctg cgtatcatca ccgataacct caaacaggaa gagcacgcca
     6061 ttgtccaggt ggaagagatg caggcggtga atgccgtgct gtatggcaaa tacaccatgg
     6121 aaggagagca gtttgaaact gttgaggtcg attttggccg gtcggtggga aataacatta
     6181 cgcaggcagg agggacggag tggagcgctc aggaccgcga tacctttgat ccgacgcatg
     6241 atctggatgc gtactgtgat tttgcttccg gcaccatcaa tattgccatt atggatggta
     6301 aggtctggcg tctgctgaat ggctttaagc tgttccgcga aaaactggat acccgtcgtg
     6361 gctcaaattc acagctggaa acggcagtga aagacctggg ggcggtggtg tccttcaaag
     6421 ggtattatgg tgatctggca atcgtggtgg caaaaacgtc ctatgtggca gaggacggta
     6481 ccgaaaaacg ttatctgccg gacggcacac tggtactggg aaatacggcc gcagagggta
     6541 ttcgttgcta cggtgccatt caggatgcgc aggcattgtc tgaaggcgtg gtggcgtctt
     6601 cccgttatcc gaaacactgg ctgaccgtgg gtgatccggc gcgtgaattc accatgacgc
     6661 agtccgcgcc gctgatggtg ctgccggatc cggatgagtt tgtggtggtg caggtgaaat
     6721 aatccgtgag cgggagctaa atgctcccgt ttttcattca atcaggagcg ggtatggcta
     6781 cgaaagaaga aaacctgaaa cgtctttgtg agctggctga gaggctgggg cgtgaaccgg
     6841 atgtctccgg tagtgcggca gatattgcgc agcgagtggc ggaactggag gaagagcttg
     6901 gagatgcggg ggaggtcgca gagccggata cgtactcacc atcgaaagat gatccgaccg
     6961 cgcataaagg cgagtcagtt cctgaacagc caggcattgt taccggagac gaaacggggc
     7021 aggttacggt ggtggcactg gcgacactgc atacaaaatg taatggttca gtgatatttg
     7081 tttcaccggg aacgtcgttt cgtgtttctg ctgaggtggc tgccagcatg gcggcaggtg
     7141 gtctggcaaa aaggcagtaa caggagatgt cgtggacggt ttcgataatc tgtttgatgc
     7201 tgcgcttgcc ggtgtcaatg aagtcattct ccgtgatatg gggatcagcg ccgtcattac
     7261 gtccggagaa cttgagggga cacacctgac aggggttttt gatgatcccg aaagtatttc
     7321 ttttgttgcc ggggggatcc ggcttgagga ttcctcaccg tgcctgtttg tgaaaacggc
     7381 agatatatcg cagttacgcc gtcaggatac gttaactatc ggggatgatt ctttctttgt
     7441 ggatcgtatt actccggatg acgggggatg ctgttatatc cggttaaggc ggggcagtcc
     7501 tgccccacag aataatgcca gaatgaggcg atatgatgaa gggacttgag aatgccatcc
     7561 gcaatctgaa cagcctcgat acccgcatgg tgccacagac cagcgcatgg gcggtaaacc
     7621 gtgtggcggc aaagattgtt tctgtcgcca cacggcaggt tgcgcagaat accgttgccg
     7681 gggataacca ggtgaaaggt attcccctga aaacggtcag ggagcgtgtc aggctgctta
     7741 aagccagccc ctcaggaaaa atgtatgccc gcatgcgtgt taaccggggt aacctgcccg
     7801 ccattaagct gggtaccgca caggtcagac tggcccgctc ccggcatggc agcaactcac
     7861 gtcatcgtgg cagcgtactg aaagtgggga aatacctttt ccgggatgcg tttattcagc
     7921 aactggcgaa tggtcgctgg catgtgatgc ggcgtattga cggcaaaaat cgttacccca
     7981 ttgatgtggt gaaaatcccg
//
LOCUS       DAJTOW010000151         7744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTOW010000151 DAJTOW010000000
VERSION     DAJTOW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.151"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO244_003697"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO244_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5665371.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO244_003698"
     CDS             1102..2562
                     /locus_tag="OO244_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5665372.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIHKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO244_003699"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO244_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5665373.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO244_003700"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO244_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5665374.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO244_003701"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO244_003701"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5665375.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO244_003702"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO244_003702"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5665376.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO244_003703"
     CDS             complement(6727..7245)
                     /locus_tag="OO244_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5665377.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7744
                     /gene="ydeI"
                     /locus_tag="OO244_003704"
     CDS             7521..>7744
                     /gene="ydeI"
                     /locus_tag="OO244_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5665378.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1940 a         1812 c         1956 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc ataaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aagg
//
LOCUS       DAJTOW010000152         7670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTOW010000152 DAJTOW010000000
VERSION     DAJTOW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.152"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..721
                     /locus_tag="OO244_003705"
     CDS             <1..721
                     /locus_tag="OO244_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5665379.1"
                     /translation="EYYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIK
                     CQPFECPDHFYVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAV
                     KSWAHKVPTAWPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITEL
                     RDMMLDGRFKVFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMR
                     DIKKPKEKKIPAPIRPIARRT"
     gene            722..2887
                     /locus_tag="OO244_003706"
     CDS             722..2887
                     /locus_tag="OO244_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5665380.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            2901..3812
                     /locus_tag="OO244_003707"
     CDS             2901..3812
                     /locus_tag="OO244_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5665381.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            3812..5107
                     /locus_tag="OO244_003708"
     CDS             3812..5107
                     /locus_tag="OO244_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5665382.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5152..5400
                     /locus_tag="OO244_003709"
     CDS             5152..5400
                     /locus_tag="OO244_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665383.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEPEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5378..5878
                     /locus_tag="OO244_003710"
     CDS             5378..5878
                     /locus_tag="OO244_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5665384.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            5878..7296
                     /locus_tag="OO244_003711"
     CDS             5878..7296
                     /locus_tag="OO244_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5665385.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7296..>7670
                     /locus_tag="OO244_003712"
     CDS             7296..>7670
                     /locus_tag="OO244_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665386.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQ"
BASE COUNT         2062 a         1889 c         2039 g         1680 t
ORIGIN      
        1 tgagtactat accgacgagc agaaagagca aatcatcgca tcatatcccg agcatgagag
       61 agaggcgcgt gctcgcggta ttcctacgat gggtagtggt cgaatcttcc agataccgga
      121 agagacgatt aagtgtcagc cgttcgagtg tcctgatcac ttctacgtaa ttggcgggat
      181 ggatttcgga tgggatcacc cacaggcgca agttcagctt tggtgggata aggaggcaga
      241 cacaatctac gtttcacgcg tgtggaaggc gaaagaaaaa acagccgttc aggcgtgggg
      301 agccgttaaa tcatgggcgc ataaagtgcc aaccgcatgg cctcatgatg gaaaccagca
      361 cgagaagggc ggcggtgagc agctcaaagg gcagtatgca gacgctggat ttatgatgtt
      421 gcaggagcat gcgacatggc ctgatggcgg taacgctgtt gagccaggca tcactgaatt
      481 gcgcgacatg atgctcgatg gtcgcttcaa agtattcaat acctgtgagc cattctttga
      541 ggagttccgc ctctatcacc gtgatgaaaa cgggaaaatc gtcaagctta acgacgacgt
      601 gctatccgcc gttcgctatg catacatgat gcgccgcttc gcaaaaatga tgcgcgacat
      661 caaaaaacca aaagagaaaa agataccagc cccaatcagg cccatcgcac ggagaactta
      721 aatggccgac gaaaacagac tcaattccat tctgtgtaag tttgacgcag actggatggc
      781 gagcgatgaa gccagaaccg aggcgacaaa tgacctgtat tttagccgag tgtcgcaatg
      841 ggatgactgg ctatcaaact acactaccct gcaatatcgc ggacaattcg atgttgttcg
      901 cccggaggtc aggaaactgg tcgcagagat gcgccggaac cctgtcgacg ttctcttccg
      961 acccaaagac ggcgctaatc ctgatgcagc cgatgtattg atggggatgt atcgtactga
     1021 tatgcgccat aacacggcaa agattgccgt taacgttggc gttcgtgagc agatagagtc
     1081 aggcgttggt gcatggcgtc tggtcacgca gtacgaagac aacgatccaa caagcaacaa
     1141 tcaggtaatc cgacgcctgc caatccatga agcctgctca cacgtcatat gggacgccaa
     1201 cagcaagcag atggataaga gcgacgctaa gcactgcacg gtgattaacg ccttgtcgcg
     1261 caatggctgg aaagagttcg cagaggatta cggtattgac ccggataccc tgccatcttt
     1321 ccagaatcct aacgatacat ggctattccc gtgggtgtcg aatgatgtcg tctacgtcgc
     1381 tgagtattac gaggtagaag aggagaagga gaaggtcttc atctaccgcg acccgctgac
     1441 aggtgagccg gtcagctatt accagcagga tatcaaagac gtcatcgacg acctggctaa
     1501 tcgtggattc attaaggtag cagagcgcaa ggtgaagcgt cggcgtgtgt ataagtcgat
     1561 catcacctgt acgcagatac tgaaagaccg cgagaagata gccggagagc atattccaat
     1621 cgttccagtg tatggcgaat ggtcattcgc tggtgacaag gagtgctacg agggtgtggt
     1681 aaggctgacg aaagacggtc aacgccttcg taacatgatt atgtcgttca acgccgatat
     1741 tgttgctcgg tcaccgaaga agaaaccgac cttcttccct gagcaaatcg aaggctacga
     1801 atacatgtac ggcggaaatg atgactatcc gtactatctg cagaacagga ccgatgaaaa
     1861 cggtaacgac ctgccgattg gtccaatctc ctacatggaa aaccctgaag tgccgcaagc
     1921 caacgcttac atgcttgagg ctgccaccaa cgcagtgaaa gaagtggcta gtcttggcgt
     1981 ggatgcgcag gcggcaaatg gtcaggtcgc ttttgatacc gtcaatcaac tgaacatgcg
     2041 ggcagacctt gagacatacg tgtttcagga taacctggct accgcaatgc gacgtgatgg
     2101 cgagatttat gcctcaatgg tcaacgatat ttatgacgtt cctcgtcatg taacgctgac
     2161 acttgaagat ggaagcgaga aagacgttca actctatgcg caagttgtcg attaccagtc
     2221 cggcaatgtg gtcacactca acgacattcg cggtcgatat gagtgctata cagacgttgg
     2281 gccatccttc cagagtatga aggaacagaa ccgcgcagag attcaggagt tgctcaccaa
     2341 ggttccgcaa ggtactccag agttccagat gctgatgctg caatacttca cgctgcttga
     2401 cggtaaaggc gtcgagatga tgcgagagta cgcgaacaag caactggtga tgatggggct
     2461 gaagaaacca gaaacacctg aagagatgga gatggtgcag caggcacaac aacagccgca
     2521 gcagccatca gcagagcaaa ttcaggcgca gggtatcctt ctgcaaggtc aggctgaatt
     2581 gctcaaggca gagaaccaac aggcgcagat tcaggttgaa gctgccaagg ttgaagccca
     2641 aaaccaactc aacgccgcga agattgcaga aatattcaac aatatggacc tcgacaagca
     2701 ggcagaactg cgtgagtacc tcaggctcgt aggtcaattc cagcaacagc gcagcaaaga
     2761 tgctcgtgct aacgctgagc tgcttcttaa agatgcagac cagactcatt cacaacgcat
     2821 ggatttcgcg aatcttatgc gtcaagttca aatcccctcc ggcggagtag ccgagacacc
     2881 tcaataagag agagttaatc atggaccaaa ccaccgacat tcaggcttct gaagaattaa
     2941 ccctgcccgg caatcatgca gcggcatctg ctgatggctt agttgtcgat aatgccaacg
     3001 acagcgcagg tcaggaagaa ggctttgaga ttgtcctgaa agacgatgag aaaccaaaac
     3061 aagacccggc aactaatgct gaatttgccc gtcgccgcat cgaacgcaaa cgccagcgtg
     3121 agcttgagca gcagatggaa gcggttaagc gtggagagtt gccggagcac ctgcgggtga
     3181 accctgagtt accaaaacaa ccagacccta acgattatct ttccgaagat gcactggcta
     3241 agtacgacta tgaccagagc cgcgcactgg ctgccttcca gcaggcaaac agtgaatggc
     3301 agatcaaggc tatggacgca cgaagccagg ctgtcgccga gcagggtcgc aaaactcagg
     3361 agttcaccca gcaatcagcg caatacgtcg aggcggcccg taagcactac gaggcagcgg
     3421 aaaagctcaa tatccctgac tatcaggaga aagaggatgc attcatgcaa ctggtgccgc
     3481 cagcagtcgg tgccgacatc atgcgcctct tcccggagaa atccgccgct ctcatgtatc
     3541 accttggtgc taatcctgag aaaacacgcc agttgctggc gatggacggg caatccgcgc
     3601 tgattgaact cactcgactg tcagaacgtt taactctcaa gcctcgagcc aagcctgttt
     3661 cagaagcccc gttacctgat gaacccattc agggacatgc tgttgctgca aatatctctg
     3721 cgattgaaaa gcagatggag gcggcagcaa acaaagggga tgtagagacg taccgcaagc
     3781 tcaaggcgca actgaataaa ggaattcgat aatggcatta aatgaaggtc aactggtcac
     3841 gtatgctctg gatgaaatca tcggaaccgt ccagaacctg acgccaatgg cgtcaaaagt
     3901 gacaaaatac ccccctccgg cagaatccat gcagcgttca agcaacaccg tgtggatgcc
     3961 tgttgagcag gaagcgccaa cccagactgg ctgggattta actggcaacg caaccgggat
     4021 tctggaactc tccgtgaagt gcaacatggg cgatccggat aacgatttct tcgagcttcg
     4081 tgcagatgac ctgcgtgatg agcgttctta ccgtcgccgc atccaggcat ccgccaaaaa
     4141 actggcgaat aacattgagt cagcgattgc caaacaggca actgaaatgg gctcgcttgt
     4201 tgttcacgat acccgcgcaa ttggtccatc gactggcctg cctggctggg attttgtgtc
     4261 tgatgcagag cgactgatgt tctcccgtga gctaaaccgc gacatgggca tcagttactt
     4321 cctgaaccct gacgattacc gcaaagcagg ccgcaacctg gtagatggtg acatcttcgg
     4381 gcgcgttcct gaagaagcat atcgaaacgg tactattcag cgtcagattg ctggctttga
     4441 tgaaattctt cgctcaccga aacttccggc agttaccaag tcaaccgcga ctggtgtaac
     4501 tgtttctggt gcgcagaagt ttaagccgca ggcatacacc cttgataccg atggtaacaa
     4561 agagaacgtc gacaaccgtg ttgcaacggt gaccgtatcc tccaccaccg gatttaagcg
     4621 cggcgacaaa atcagcttca ttggtgtgaa gttcctgtct cagatggcga agaatgtgct
     4681 gactgatgac gcgactttct caatcacccg tgtgatcgat ggtactcaca tcgaaatcac
     4741 gccgaagccg attgcgctgg atgacgcgtc actgacaaaa gaagagaagg cttacgctaa
     4801 cgtaaacacc tctcttgctg ataccactcc ggtaaacgtt ctgaacgtgg caacaaccac
     4861 cgctaacgtg ttctgggctg atgactcaat ccgcctgctg tctcagccga tcccggtaac
     4921 ccatgaactg tttgctggca tgaaaacgtc ttccttcagc attccaggta ttggtgttaa
     4981 cggcatcttc gcaacgcagg gtgatatcaa cactctgtct ggtaagtgcc gtattgctgt
     5041 gtggtattca gcatgtgctg tacgaccaga ggcaattggt gttggtctgc ctaaccagac
     5101 cgcgtgataa ccagagggag cttcggctcc cgtttttatc tggagacaag catgacacac
     5161 atgatctttc gtcatggcga catgaagaag tggaaaggcg ttggctacga ctttgaaatc
     5221 gtgaaagccg aagagcttca ggaatatctg gatgctggct ggttttcaca tcctgatgac
     5281 cttttgaagg atgttgcaga gccagagcca gagccagagc cagagccaga agaaaaacag
     5341 cgtaaaaagc ctggtcgaaa acctaaggcg gcagcagatg aacctgacaa cgaagggtga
     5401 tttagttctt gcggcattac gtaagctcgg tgttgcatca aatgccacgt taaccgatgt
     5461 cgaaccgcag tctatggaag acggcgtcaa cgaccttgaa atgatgatgg ctgaatggct
     5521 tggcggtgat gcgtcacctg gtatcaacgt tggctacatt tttgctgatg cagatgttgc
     5581 tccggatccg ggcgatgagc acggtttatc aaataacgct atcaatgccg tcattttcaa
     5641 ccttgcctgc cgaattgctc cagattatgc gctggaagcg tctgcaaaac ttataaccac
     5701 tgccagatac gggaaagagc gactcgtcaa actgtctgca atggacaggg caaaagcagc
     5761 taaatgtaag tccggttatc caaaccgtat gcctgttggt agtggtaacc agttggcgaa
     5821 gtggaatggt tggaattact tccaccgaaa ggaaccttgc gataacggga gcgaataatg
     5881 cctattcagc aacttccgct tatgaaaggt gtcggcaaag acttccgaaa cgccgactat
     5941 atcgactatc tgccagtgaa tatgctggct acacccaaag aaatcctgaa cagcagcgga
     6001 tatcttcgct cattcccggg cattgccaaa cgttctgacg tgaacggtgt atctcgcggc
     6061 gtcgagtaca acatggcgca gaatgctgtt tatcgcgtgt gtggcggcaa gctgtacaaa
     6121 ggagaaagtg aagtcggtga tgttgccgga agtggtcgtg tatcaatggc gcatggtcgg
     6181 acatcacagg cggtaggcgt taatggtcaa ctggtcgagt atcgctatga tggcacggtc
     6241 aaaaccgtct caaactggcc tacagacagt ggattcactc agtatgagtt aggttcagtt
     6301 cgcgacatta cgcgcttacg tgggcgttat gcgtggtcaa aagacggaac tgattcatgg
     6361 tttatcactg accttgaaga cgaatctcat cctgaccgtt acagcgcaca atatcgtgcc
     6421 gagtctcagc ctgacggcat catcggtatc gggacatggc gagacttcat cgtctgcttt
     6481 ggttcatcga ctattgaata tttctccctg actggcgcaa ccaccgttgg tgccgctttg
     6541 tatgttgcac aaccatcgct gatggtgcag aaaggcattg ccggaactta ctgcaaaacg
     6601 ccattcgctg attcgtatgc gtttatcagc aatccggcaa cgggtgcgcc gtctgtgtat
     6661 atcatcgact ccggtcaggt gtcaccaatt gccagcgcga gcattgagaa aatcctccgc
     6721 tcctacactg ctgatgaact ggctgatggt gtgatggaat cgctgcgatt tgatgctcat
     6781 gagttgctga ttatccacct tccgcgtcac gtcctcgtgt acgacgcatc ttcaagcgcc
     6841 aatggcccac aatggtgtgt gctgaaaaca ggtctgtatg acgatgtgta ccgcgctatc
     6901 gacttcgttt acgaaggcaa tcagataacg tgcggcgata agctggaatc tgttaccggg
     6961 aaactgcaat tcgatatcag cagccagtac gacaagcaac aggaacacct gctgtttact
     7021 ccactcttca aagcggataa cgccagagtg ttcgaccttg aggttgaatc gtcaactggc
     7081 gttgcgcagt acgccgaccg cctttttctc tctgcaacca ctgactgcat caattacggg
     7141 cgtgagcaga tgattgagca gaatgaaccg ttcgtttacg acaagcgtgt tttgtggaag
     7201 cgagtagggc gcatcaggaa aaatgtcggc ttcaaattgc gcgttatcac gaagtcacct
     7261 gtcactctgt ctggcgctca gataaggatt gagtaatggc tgattcgaat ctcaatgtgc
     7321 cggtaatcat ccaagctacg cggctcgata catcagtcct tccacgcaat atcttctcgc
     7381 agtcgtatct gctttacgtt atcgcacagg gcactgatgt tggtaacgtg gctaacaaag
     7441 ccaacgaggc cggacagggc gcttatgatg cacaggtcag gaacgatgag caggatgtga
     7501 ttctcgctga ccatgagcag cgaatttctg ctgcggaagc aacgcttgtt aatcatgagg
     7561 agcgaatcag acaggcagaa tcaactcttc aggaccatga aacacgaata gctcagaatg
     7621 aaagcgatat ttcgtcgctt gacacaagag ttcagtcgct ggagtcgcag
//
LOCUS       DAJTOW010000153         7426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTOW010000153 DAJTOW010000000
VERSION     DAJTOW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.153"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            170..1273
                     /gene="proB"
                     /locus_tag="OO244_003713"
     CDS             170..1273
                     /gene="proB"
                     /locus_tag="OO244_003713"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5665387.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1285..2538
                     /gene="proA"
                     /locus_tag="OO244_003714"
     CDS             1285..2538
                     /gene="proA"
                     /locus_tag="OO244_003714"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5665388.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2653..2728
                     /locus_tag="OO244_003715"
     tRNA            2653..2728
                     /locus_tag="OO244_003715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Thr,seq:cgt)
     gene            complement(2743..3903)
                     /locus_tag="OO244_003716"
     CDS             complement(2743..3903)
                     /locus_tag="OO244_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5665389.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFRETILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGKPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPA
                     YRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALK
                     EMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3795..4127)
                     /locus_tag="OO244_003717"
                     /pseudo
     CDS             complement(3795..4127)
                     /locus_tag="OO244_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4227..4571)
                     /locus_tag="OO244_003718"
     CDS             complement(4227..4571)
                     /locus_tag="OO244_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665390.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFGKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(4673..4852)
                     /locus_tag="OO244_003719"
     CDS             complement(4673..4852)
                     /locus_tag="OO244_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HCR5665391.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKESEGNEKS"
     gene            complement(4949..5608)
                     /locus_tag="OO244_003720"
     CDS             complement(4949..5608)
                     /locus_tag="OO244_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5665392.1"
                     /translation="MSTITREWLQQAINDYESVRDELPFGLDDYQGNILAALRIALAS
                     LEAEPIGAFHIAEQQVDGTSDYIKDGEWPIDNGIIEVYAAPPVPVVPAALPENDDEDG
                     HDIDYLEPSEVYALGRTAGWNACRAAMLQGKSEQPQNAQQNIPENIPGGNSPVTPDGW
                     ISCSDRMPNEEDVLVYCSDTKEQMVGFHKGKGLFQFFYMNGVEGVCEPSHWMPLPEPP
                     L"
     gene            complement(5605..6339)
                     /locus_tag="OO244_003721"
     CDS             complement(5605..6339)
                     /locus_tag="OO244_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5665393.1"
                     /translation="MSKIDYQALRAKAEKATCGEWSLEYGEGRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQIEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELGGKDSKIANLTAERDALREGEMGDARHSNTRAAADIYFQLVEE
                     CEIPAGGSLVEYVDDMREKLEAAEKRIAELELREVVLPQCYSMLHRVDFDEPYHTEMV
                     YRQHQVLEALHNAGINVTEACKGEAS"
     gene            complement(6326..6559)
                     /locus_tag="OO244_003722"
     CDS             complement(6326..6559)
                     /locus_tag="OO244_003722"
                     /inference="COORDINATES: protein motif:HMM:NF025712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HCR5665394.1"
                     /translation="MSNLLPCPFCGGAAHVASEADHPEYGSGGRFYFVRCGTCRAQSG
                     SKYAAPGNDCAIFYSEVRAEWNQRAKEANSEQD"
     gene            complement(6556..7230)
                     /locus_tag="OO244_003723"
     CDS             complement(6556..7230)
                     /locus_tag="OO244_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665395.1"
                     /translation="MKQMTLIEMDGFLKGKCIPRDLNVNETNAEYLVRKFGELESKLE
                     TALRECRSAGITIDNLDAKCAALAAESAVMKAGHSYFSYGSEHNFEWHKTAEEAIESA
                     EAAIDDYRGDACDGWSEEVDSICWGIIMQSSTKVGERPRNEDDCCDPAIDTICDYALL
                     PNIETPATDAFLAEVRAKAFDDLCAAFVKHASVSGLDDGDCVTVKEATDALLHCAEQL
                     HKGVHS"
     gene            complement(7227..7391)
                     /locus_tag="OO244_003724"
     CDS             complement(7227..7391)
                     /locus_tag="OO244_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR5665396.1"
                     /translation="MRGLAYNPGILPAEMIIRQRLKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
BASE COUNT         1704 a         1946 c         1797 g         1979 t
ORIGIN      
        1 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
       61 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
      121 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
      181 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
      241 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
      301 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
      361 actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
      421 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
      481 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
      541 gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
      601 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
      661 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gaaatccgca
      721 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
      781 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
      841 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
      901 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
      961 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     1021 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     1081 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     1141 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     1201 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     1261 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     1321 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     1381 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     1441 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     1501 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     1561 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     1621 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     1681 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     1741 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     1801 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     1861 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     1921 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagt
     1981 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     2041 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     2101 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     2161 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     2221 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     2281 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     2341 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     2401 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     2461 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     2521 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     2581 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     2641 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     2701 gtaggttcga ctcctattat cggcaccatt taaatcaata agttacctcg catttaagta
     2761 aaccacgttc tcctcttgtg ccgtatttgt gccattgcga cttataatcg catcgatttt
     2821 gcttgcgtgc tcggtgagat gcccggctga aaggtgggcg tatctttgaa ccatttcgag
     2881 agtttcccat cctcccatct ctttaagtgc aagaagagag acaccggact gaaccagcca
     2941 gcttgcccag gtatgcctca gatcatggaa gcggaagttg ctaatgcctg cccgctttaa
     3001 cgctcccttc catgccttgt tgctgtcggt tctcatcttc cttaccgctg ctgtttttgt
     3061 tccgtcgctt cggtaggcag gtttggtgtg gacaaatacc catctcttat ggagcccctg
     3121 ctgttttctt aatatctggc atgcggtttc gttaagagga actccgatcg cattgccagc
     3181 ttttgtttca tcggggtgca tccatgccat tttcttatcc agatcgacct gtgaccactc
     3241 aaggtctgta acgttggaac ggcgaaggcc tgtcgtgatt gcaaacatga ccacagggaa
     3301 gaaatgagga gcaatttctg caaacaggcg cttcgattcc tcctctgtaa gccatctgat
     3361 tcgtccattc ttaacgcgtg gtgttgatat tttgggcgcc ctgtcaagcc atccccattc
     3421 aacagccata ttgagaatag cgcgaagtat tgccagatgc cgcgtcttcg ttcctttgct
     3481 tgccagcttt ggtttatact ccggcactgg cttgccaagc cgcaaacacc tgtcccggct
     3541 catttcccag ttcaggcgat ggcggcggtt ttccatcccg tctaccgcct ccattatttt
     3601 ttctgttgtt atgtcagaga gaatggtttc tctgaagtgc aacatccaga acgatataat
     3661 gctcttgtca tcatcaatgg acttcttatc cgatttctca cgcagccacc gtatgcaggc
     3721 ttccttgaat agctttttcg gcgattcccc gagatttttt actctccacg cttctgcttt
     3781 cagacgatcg tgaagttctt gcgcttgcct tttgtccgat gtttcaagag agcgtctaac
     3841 tcttgatcca tctggcgcga cgaaatcgca gtgccacgtg ccaccgcgta gtttgattga
     3901 catgctttaa cctcctgcac atcaaccgca ttcaccgcgc tattgtgtct cacagactta
     3961 agcgccgcaa tgcagtctga cttgcaaatg cgatatggac ttttaggttt atctggattt
     4021 atctttgcgg cctgaagtcg tccacttcgt atccactgtg tgatagtgcc tttgtctacc
     4081 ttcagatacg acgcagcttc ttcacgagtg aagatttctt cttccacctg gaatctccat
     4141 ttgttggatt gacatgattg cggtaggtct ggatatcttg agaaatgaac tggcctcatc
     4201 gagtgtgagg ctgtgtgatt ccatagttag tccttgcgta gctcgctgat tcttctgtaa
     4261 gtctctggtg ctttgtttcc gtgtatcttc atttcagact tcaacagagc aacgagggaa
     4321 tcccattcgt tgaggatgcc tttgaatgcc ggaacgcgct ttgcaacctt gccgaatgaa
     4381 tctctgattt ctggaatctg ctcaacaagt gcaacgcatc gtctgaaatc ggctgcgtca
     4441 tggggagcgc cgaagtgatg accatagata ttctttttca gcccacatgc gattgaggca
     4501 agagttgcgc tactgatgcc aacatcgcca gtcgattgcc atttcaaaac cttcatagcc
     4561 aaatctgaca tttcttgtct ccaataaaaa aaccgccatc aggcggcttg gtgttctttc
     4621 agttcttcaa ttcgaatatt ggttacattg ttttcatata tgaataaata aattagcttt
     4681 tttcgttgcc ttcgctttct ttattaattt taacaaactc gtttttacca cgctctccaa
     4741 atgcgtcttt agagtcgttg tatccgcaat cgcagcacac ataatctcca gaccatccac
     4801 gcattgtttt ttcttttgca atatttccag aaccgcattt tggacaagac atgtcactac
     4861 ctccaaagca tgagtgagat gacaacgtaa cattgattgg agattaacaa tagattgctg
     4921 atgtaaaaga tatgtataag cttcgctttc aaagtggagg ctctggtagc ggcatccagt
     4981 gtgacggctc acataccccc tcaacaccat tcatgtaaaa gaattgaaat aaccctttac
     5041 ctttgtgaaa ccctaccatc tgctcttttg tgtctgagca ataaaccaaa acatcttctt
     5101 cgtttggcat tcgatcacta cagcttatcc aaccatcagg agttaccgga gagttgccac
     5161 cgggaatatt ttccggaata ttttgttgtg cgttttgtgg ttgttcggat ttaccctgaa
     5221 gcatggcggc gcggcaggcg ttccagccag ctgttcgccc aagcgcgtaa acttcagatg
     5281 gctcaagata atcaatgtca tgcccatcct catcgtcgtt ctcaggtaat gcagcaggta
     5341 ctaccggtac tggcggagcg gcgtagactt caataatccc attatcaata ggccattccc
     5401 catccttgat gtagtcactt gtgccgtcaa cttgctgttc tgcaatgtgg aatgcgccaa
     5461 taggctctgc ttccagcgat gccagtgcga tacgcagggc agccaggata ttcccctggt
     5521 aatcatcaag cccgaaagga agctcatcac gaacgctttc ataatcgttg atagcctgct
     5581 gcagccattc tcttgtgata gtgctcatga tgcctctcct ttacatgctt cggtgacgtt
     5641 tattccagcg ttgtgcagtg cctcaagaac ctgatgctgc ctgtaaacca tttcagtgtg
     5701 gtaaggctcg tcaaaatcga cgcgatgcaa catgctatag cattgcggaa gcacaacctc
     5761 ccgcaactcc agctcagcaa tgcgcttctc tgcggcttcc agcttctcgc gcatatcgtc
     5821 aacgtactcg accagagatc cgccagcagg aatttcgcac tcctcgacca gttggaagta
     5881 gatatcagct gcggcccgtg tgttgctatg cctagcgtcg cccatctcac cttcacgaag
     5941 agcatcgcgt tcggcggtaa gattggctat tttgctgtct ttgcctccca gctcaactct
     6001 cagcttccct accgttagcg caatatcctc gttctcctgg tcgcggcgtt tgatgtattg
     6061 ctggtttctt tcccgttcat ccagcagcgc cagcacggta gctggattgg ctgcggcgat
     6121 gaattcagca ttggcctgct gttctatttg gaaatcttca tcgaaaccgc tttcaggatg
     6181 cgctccttca attctgcaaa tgggaatata tccagcagcc tcgcgatgaa ttagcgcatc
     6241 atcaccatca aatcggccct ctccatattc gagcgaccac tcgccacacg ttgctttctc
     6301 tgccttggca cgcagtgcct gatagtcaat cttgctcact gtttgcctcc tttgctcgct
     6361 gattccactc tgctctaacc tctgaataaa aaatcgcgca gtcatttcca ggcgctgcat
     6421 atttgctacc agattgagcg cgacacgtac cgcatcgaac gaaatagaat cgaccgccag
     6481 agccatattc agggtgatct gcttcgctgg caacgtgcgc tgcaccgcca cagaatggac
     6541 atggtagtag gttgctcatg aatgcactcc cttgtgaagt tgttccgcac aatgcagcag
     6601 tgcgtcagtc gcctctttca ccgtaacgca gtcgccatcg tccagcccgg acaccgacgc
     6661 gtgcttaacg aacgccgcgc aaaggtcatc aaacgcctta gcccggactt cagccaggaa
     6721 agcatcggtg gctggggttt cgatattagg cagaagcgca taatcgcaaa tcgtatcgat
     6781 cgcaggatcg caacagtcat cctcgttgcg tggtcgttcg ccaaccttcg ttgacgattg
     6841 catgatgatt ccccagcaaa tactatcgac ctcttcgctc catccatcgc aagcatcacc
     6901 gcgatagtcg tcaattgcag cctcagccga ttcgatagct tcctcagcag ttttgtgcca
     6961 ttcgaaatta tgctcagagc catatgagaa atatgaatgt cccgccttca tcaccgcact
     7021 ctccgctgcc agcgccgcgc acttggcatc aaggttatca atcgtgattc cagcagaacg
     7081 acactcccgc aacgccgttt ctagttttga ttcaagttca ccgaacttac gcaccagata
     7141 ttcagcgttt gtttcgttaa catttaaatc tcgggggatg catttacctt tcagaaatcc
     7201 atccatctca attagtgtca tttgtttcat tttttcccac tccgccacat cgcattcaga
     7261 tatttgtttt gatttactga cggaaaagaa tttctcttaa gcaattcctc tctcgatggc
     7321 attggcttta ggcgttggcg aataatcatt tctgccggaa gaatgccggg attgtatgca
     7381 agtcctctca tggtaaattc ctcagtcatt actgatagcg ccatag
//
LOCUS       DAJTOW010000154         7253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTOW010000154 DAJTOW010000000
VERSION     DAJTOW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.154"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..904
                     /locus_tag="OO244_003725"
                     /pseudo
     CDS             1..904
                     /locus_tag="OO244_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            909..1874
                     /gene="rbsC"
                     /locus_tag="OO244_003726"
                     /pseudo
     CDS             909..1874
                     /gene="rbsC"
                     /locus_tag="OO244_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
     gene            1899..2789
                     /gene="rbsB"
                     /locus_tag="OO244_003727"
                     /pseudo
     CDS             1899..2789
                     /gene="rbsB"
                     /locus_tag="OO244_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2915..3844
                     /gene="rbsK"
                     /locus_tag="OO244_003728"
     CDS             2915..3844
                     /gene="rbsK"
                     /locus_tag="OO244_003728"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5665397.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3848..4840
                     /gene="rbsR"
                     /locus_tag="OO244_003729"
     CDS             3848..4840
                     /gene="rbsR"
                     /locus_tag="OO244_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5665398.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4806..6233)
                     /gene="mdtD"
                     /locus_tag="OO244_003730"
     CDS             complement(4806..6233)
                     /gene="mdtD"
                     /locus_tag="OO244_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5665399.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6256..6948)
                     /locus_tag="OO244_003731"
     CDS             complement(6256..6948)
                     /locus_tag="OO244_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5665400.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1839 a         1750 c         1919 g         1745 t
ORIGIN      
        1 tgaaagaaat cttcgagatt tgcgatgacg ttaccgtttt tcgtgatggg caatttattg
       61 ctgagcgcga agtggcatca ctgaccgaag attcgctgat tgagatgatg gtgggccgca
      121 agctggaaga tcaatatcca cacctggaca aagcgccagg agatatccgc ctgaaagtcg
      181 ataatctctg cggacctggc gttaacgatg tctcttttac tttacgcaaa ggcgaaattc
      241 ttggcgtctc tggtttgatg ggcgcaggtc gtaccgaact gatgaaagtg ctctacggcg
      301 cactgccgcg caccagcggt tacgtcaccc tggatgggca tgaagtcgtt acccgttcac
      361 cgcaggatgg cctggcaaac ggcattgtgt atatctccga agaccgtaaa cgtgacggtt
      421 tagtgctggg catgtcagta aaagagaaca tgtcgctgac agcgctgcgc tacttcagcc
      481 gcgctggcgg cagtttgaag catgccgatg aacagcaggc tgtgagtgat ttcattcgtc
      541 tgtttaatgt gaaaacgccg tcgatggaac aggcaattgg tctgctttcc ggtggcaatc
      601 agcaaaaagt ggcgattgcc cgcggtctga tgacaccccc aaagtattga tccttgatga
      661 gcctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt
      721 caaagccgat ggcttgagta tcattctggt gtcatcggag atgccagaag tattaggcat
      781 gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca
      841 ggccacccag gaagtgttaa tggctgtcgc tgtgggcaag cttaatcgcg tgaatcagga
      901 gtaaaaaaat gataacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga
      961 tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgtgaagcc
     1021 cgaacttttt caccatcaat aacttattca atattctcca gcaaacttcg gtgaacgcca
     1081 ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catagacttg tcggtaggtt
     1141 ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc
     1201 tggtggctgt cgctgctgct ctcacgttag gtgccgcaat tggtgcggta accggggtga
     1261 ttgtagcgaa aggtcgcgtc caggcgttta tcgctacgct ggttatgatg cttttactgc
     1321 gcggcgtgac catggtttat accaacggta gcccagtgaa taccggcttt actgagaacg
     1381 ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga
     1441 tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt
     1501 acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc aacgtcaata
     1561 aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg
     1621 aagtggcgcg tctctcctct gcacaaccca cggcggggac tggctatgag ctggatgcta
     1681 ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga
     1741 cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt
     1801 cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca
     1861 acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta
     1921 ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagaca
     1981 ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg aaagatggcg
     2041 cgcagaaaga ggcggataaa cttggctata acctggtggt gctggactcc cagaacaacc
     2101 cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga
     2161 ttaacccgac cgactccgac gcagtgggta atgctgtgaa gatggctaac taggcgaaca
     2221 ttccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgcat
     2281 ctgataacgt actgggcggc aaaatcgccg gtgattacat cgcgaagaaa gcgggtgaag
     2341 gtgccaaagt tatcgagctg caaggcattg ccggtacatc cgcagcccgt gaacgtggcg
     2401 aaggcttcca gctggccgtt gctgctcaca agtttaatgt tcttgccagc cagccagcag
     2461 attttgatcg caccaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg
     2521 ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc gcactgcaaa
     2581 ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag
     2641 cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga
     2701 aaggcgttga aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg
     2761 ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac
     2821 ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc
     2881 taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt
     2941 tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga
     3001 aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt
     3061 ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat
     3121 tggcgagagc gttcgccagc agctcgccac tgataacatc gatattactc cggtcagcgt
     3181 gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt
     3241 catcggtatt catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg
     3301 tgagcgtatt gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt
     3361 gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc
     3421 ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac
     3481 ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc
     3541 gcaggtactg catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt
     3601 atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga
     3661 taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa
     3721 accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa
     3781 aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag
     3841 gtgacgcttg gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt
     3901 ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc
     3961 ggcgattaaa gaactcaatt acgcgccatc agctctggcg cgtagcctca aactcaatca
     4021 aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt
     4081 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga
     4141 aggcgatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
     4201 cttactgtta ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc
     4261 gacagtgcct accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca
     4321 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
     4381 ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg
     4441 ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
     4501 tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg
     4561 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
     4621 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
     4681 ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
     4741 gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt
     4801 acaacttact ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc
     4861 aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata
     4921 atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct
     4981 tcataaatgc gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc
     5041 gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg
     5101 atggtattca tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag
     5161 atcagcatcc atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca
     5221 ataatcaccg tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta
     5281 accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata
     5341 aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg
     5401 gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat
     5461 aaatctaatg aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt
     5521 aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg
     5581 aatagctcta ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg
     5641 atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg
     5701 cccgcaatac ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc
     5761 agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa
     5821 ttcaataccg gaagaagttc attacgagga taagcacgca gtaaggccgg ccgagcaaca
     5881 ggcatcatca ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc
     5941 gaattagaaa gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa
     6001 atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc
     6061 gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga
     6121 gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc
     6181 atcgccgcga tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt
     6241 cctgttatcg ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc
     6301 cgcgtcgcca tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat
     6361 cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc
     6421 gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc
     6481 gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa
     6541 cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg
     6601 cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc
     6661 tgttatccac caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac
     6721 ccgagtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc
     6781 ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa
     6841 aatggtgccg ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac
     6901 atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc
     6961 tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg
     7021 gcaaattgct tgttttatgg cacattaacg gggtattttg ctgaaaaaat gcgcggtcag
     7081 aaaattattt taaatttcct cttgtcaggc cgggataact ccctataata cgccaccact
     7141 gacacggaac aacggcaaat acgccgccgg gtcagcgggg ttctcctgag aattccgaca
     7201 gaaaaagcaa aaataaatga ttgactctgt agcgggaaag cgtattatgc aca
//
LOCUS       DAJTOW010000155         7212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTOW010000155 DAJTOW010000000
VERSION     DAJTOW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.155"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            51..1541
                     /locus_tag="OO244_003732"
     CDS             51..1541
                     /locus_tag="OO244_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5665401.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO244_003733"
     CDS             1593..2750
                     /locus_tag="OO244_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5665402.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO244_003734"
     CDS             complement(2716..2838)
                     /locus_tag="OO244_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665403.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO244_003735"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO244_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO244_003736"
     CDS             4764..7124
                     /locus_tag="OO244_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5665404.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDCATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         2123 a         1575 c         1527 g         1987 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc attcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gagactatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac tgcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gt
//
LOCUS       DAJTOW010000156         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTOW010000156 DAJTOW010000000
VERSION     DAJTOW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.156"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(296..1198)
                     /locus_tag="OO244_003737"
     CDS             complement(296..1198)
                     /locus_tag="OO244_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5665405.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO244_003738"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO244_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5665406.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAAGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO244_003739"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO244_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5665407.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO244_003740"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO244_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5665408.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO244_003741"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO244_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5665409.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO244_003742"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO244_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="OO244_003743"
     CDS             7090..>7183
                     /locus_tag="OO244_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5665410.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1675 a         1812 c         1877 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccagc cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAJTOW010000157         6956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTOW010000157 DAJTOW010000000
VERSION     DAJTOW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.157"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            246..1670
                     /gene="sbcB"
                     /locus_tag="OO244_003744"
     CDS             246..1670
                     /gene="sbcB"
                     /locus_tag="OO244_003744"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5665411.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1735..1941)
                     /locus_tag="OO244_003745"
                     /pseudo
     CDS             complement(1735..1941)
                     /locus_tag="OO244_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
     gene            complement(1955..3013)
                     /gene="yeeE"
                     /locus_tag="OO244_003746"
     CDS             complement(1955..3013)
                     /gene="yeeE"
                     /locus_tag="OO244_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5665412.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3192..4550)
                     /gene="plaP"
                     /locus_tag="OO244_003747"
     CDS             complement(3192..4550)
                     /gene="plaP"
                     /locus_tag="OO244_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5665413.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4540..4602)
                     /gene="yoeI"
                     /locus_tag="OO244_003748"
     CDS             complement(4540..4602)
                     /gene="yoeI"
                     /locus_tag="OO244_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5665414.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4763..5746)
                     /locus_tag="OO244_003749"
     CDS             complement(4763..5746)
                     /locus_tag="OO244_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5665415.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5792..6616)
                     /locus_tag="OO244_003750"
     CDS             complement(5792..6616)
                     /locus_tag="OO244_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5665416.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6699..6953)
                     /gene="yoeB"
                     /locus_tag="OO244_003751"
     CDS             complement(6699..6953)
                     /gene="yoeB"
                     /locus_tag="OO244_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5665417.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1889 a         1813 c         1623 g         1631 t
ORIGIN      
        1 cttcttgtgg gcccgaatat ggtttaccat aaatagtacg cagttttgtg tcgcttatag
       61 ccataaataa tatttggtac gctcagaaat aatagtttgg cacacatctg gtacacaata
      121 tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac atactcaggt
      181 gaaaaaaggg ttgcttttcg agttataaca gacagttaat taactacagt ggacaatgct
      241 agacaatgac agacacagat aaacaaccta ccttcctctt tcacgattac gaaacctttg
      301 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatgacgaat
      361 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgcggatgac tatttacccc
      421 ggcctggagc agtattaatt accggtatta ccccgcagga ggcacgggcg aaaggagaaa
      481 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
      541 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
      601 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttactggatg
      661 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
      721 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggtattgaa catagcaacg
      781 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
      841 gtcagccacg cctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
      901 tgattgatgt tccgcagatg aaacccctgg ctcacgtttc cggaatgttt ggggcatggc
      961 gtggcaatac cagctgggtg gcaccgctgg cgtggcatcc tgaaaatcgc aatgccgtaa
     1021 ttatggcgga tttggcagga gacatttcgc cattactgga gctggatatc gacacattgc
     1081 gcgagcgttt atataccgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
     1141 tggtgcatat caataaatgt ccggtactgg cccaggcgaa tacgctacgc ccggaagatg
     1201 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctaaaaatt ctgcgtgaaa
     1261 atccgcaagt gcgtgaaaaa gtggtagcga tattcgcgga agccgaaccg tttacacctt
     1321 cagataatgt ggatgcacag ctttataatg gctttttcag tgacgccgat cgtgcagcaa
     1381 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgttg
     1441 ataaacgaat tgaaaagttg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
     1501 attatgccga gcagcaacgc tggctggagc accgccacca ggtcttcacg ccagagtttt
     1561 tgcagggtta tgctgatgaa ttgcagatgc tggtacaaca atatgccgat gacaaagaga
     1621 aagtggcgct gttaaaagca ctttggcagt acgcggaaga gatcgtctaa tttgaagccg
     1681 ccgccgctgg tacggcggag gacaatgttg agtcaggctt tttgaacggt gatggctcca
     1741 cgctgcatcg ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg
     1801 tgggattgct tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc
     1861 catttctgcc agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac
     1921 atccagcttt ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc
     1981 gcgccttacg ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca
     2041 taaatacaag gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac
     2101 cgatagagca accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc
     2161 tacgtaatgt tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg
     2221 caataaatgc ctccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca
     2281 cgtcacccgc gaccagaaat tgaagaatat tggccgttgg ggaagtgact cctaacccaa
     2341 acatgcgtcc ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta
     2401 cagcagtgac aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc
     2461 gcggcggtaa ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga
     2521 gcgttatcac cagcaaaacg gtaaccaacg gccacacaga caaattaaaa gtctcagcaa
     2581 tagagttatg ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag
     2641 aacgcatcac cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc
     2701 cctcacccgc gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc
     2761 cgaaaagata cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca
     2821 gtaggcccgc ctgaattaac gcaaaaaccc ccacgctttg tgccgaaatt gcaatcagca
     2881 aggcgtaaaa catgcgatta tttttcgcga tatacatatc gcgaaaacca ccagtcaggc
     2941 aaaaacgccc acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata
     3001 tcattgaaaa cataaattaa ttaaccagat gaatgttaat ggggaaattt ttcatgactg
     3061 gtggaatgca gaccaataac caaattctct aaattagaac aaatggttat taatgaggat
     3121 ttcgataaaa aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt
     3181 agaaaagatg attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg
     3241 acgcaagcca ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt
     3301 tcttccaggt taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga
     3361 tactggaagt gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg
     3421 accgacaggt taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc
     3481 attaccaggt cgaagttgat tgccagcagg gcaatcgcgc caaccaggat gatgttcatc
     3541 gccggagtac gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca
     3601 cgacccatta cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt
     3661 acggtaatgg tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc
     3721 atgatttcag gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc
     3781 tgcaggaagt aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc
     3841 gcacgcggga tcacgcgctc tgcatctttg gtttcttccg acagattgct gatgccgtca
     3901 aagccggtaa aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca
     3961 tcgccagacc agaacggacg cgtgctcgcc agcgtaccag cgccttcacc ttcaaatacg
     4021 ccataaacaa ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca
     4081 gtgttgacgt tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc
     4141 agcgccacca caaacatcca cgatggaatg gaaggcacca gagcttcaaa atagattttc
     4201 gccagcagaa tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc
     4261 ataaagccga cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa
     4321 ggatagcggc gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg
     4381 aacgcatagg ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca
     4441 aacagcgtca tcggctgcat ataggctaga cccatcatta caaccggaac taacgtaagc
     4501 gttttacgca attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggttattctc
     4561 ctttacggtg ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc
     4621 gacaacaact gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat
     4681 gaatgattgg tttgaagcaa aaaataaccg acgctaatta aacgtcggtt tttaatcatt
     4741 ttttgacagc ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaggcttg
     4801 tcataataat ttttctttga atggctgatt ttccgtcatc agattttgca taaacaccgg
     4861 cgatcgcact atttgctaaa atttcatctc gccaccagcc gctggcaagc cctgcggtct
     4921 gttcattcca gccaatccag acaggttcaa agagagtttg ggcgaccact ttcttctcga
     4981 ttaacgcgcc actatcgaga aaacgttgcg ccagataccg tggtaaatag ccgcagccaa
     5041 ggccgctaat ttgcagctcc agtttggttt taaaatcgaa aacggtaatg gcttcttgtt
     5101 cgtcgagaag ttgtgaggcc gtagaagcgc ctgcctgcgc agtatccccc accacaatcg
     5161 cccgatatcg tttaatgata cgacggttta acggttcttc ttcctgggct aacggatgat
     5221 gaggcgcgac cgcaaagact tgttcaagat cgcccaaccg cgagaaacca aactcactgg
     5281 aagatggtgg ctcatgcatc gcgccaacga taatatccgc tcgcccctga gtcagggcat
     5341 cccaggaacc accgagtacg ccattgataa acttcaggcg cgtcacgcta tgatgttgat
     5401 aaaaagcttc aatgagaggc gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca
     5461 ccagctcgtt ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg
     5521 tatgcagaac ttcccgccct ttctctaata acatttttcc ggtgcgggtg aatttagcgc
     5581 ggtgtccgct acgatcaagc aattgaatat ttaggtcgct ttccagcttg tgaacggtgt
     5641 aactcagagc ggacggcgtc ttgtagagtt tggctgacgc cgccgcaaaa ctgccctctt
     5701 tttctatggc atcgaggatc atcaacacat ccagcagtgg tttcatacac gcccccttgc
     5761 agtgcatgcg gcgtcctgct ggcgtgatcg tttactccaa cggcattacc agcggatcgg
     5821 gatactggta ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt
     5881 tgccgctgtc cagactattt ctgaactgcg gaggttgcag ccccagtaaa cgggccatct
     5941 gcggatagaa aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc
     6001 ctttaggtgc ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat
     6061 taacaccatg ttcaccatca ggggcggttt ttccggcaaa gaagcgtccc ggatgacgtc
     6121 ccggtccgac caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca
     6181 gccagtcttc gagttcttct aatactcgtc cactgttggt taccggatta cgcggggtag
     6241 tttctttaac cgtgccttgc gcgtcgccat agacagatgt tgagctggta aaaataattc
     6301 ggggaatacg atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact
     6361 catcgccggg gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccataa
     6421 gggcatccag atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat
     6481 caatgccact cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt
     6541 gccagcctcg cgctgaaagt gacatcgcca gcggcatacc taaccaccct aacccgacaa
     6601 ttgcgacctt tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc
     6661 gtatcaaaac tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct
     6721 gcaatgagca gtgaatcatc agtaaccgcg tataccagac ggtgctcctc tgtaatgcgt
     6781 cgggaccaga aacctgacaa attatgtttc aggggttctg gtttcccctt accttcaaat
     6841 ggtgttctgc gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt
     6901 tcctgccagt acagataatc atcccatgat tcctcagacc agattagttt cactca
//
LOCUS       DAJTOW010000158         6945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTOW010000158 DAJTOW010000000
VERSION     DAJTOW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.158"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            18..1076
                     /locus_tag="OO244_003752"
     CDS             18..1076
                     /locus_tag="OO244_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5665418.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="OO244_003753"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="OO244_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5665419.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="OO244_003754"
     CDS             complement(1623..1727)
                     /locus_tag="OO244_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665420.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="OO244_003755"
     CDS             1897..2343
                     /locus_tag="OO244_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5665421.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="OO244_003756"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="OO244_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="OO244_003757"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="OO244_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="OO244_003758"
     CDS             4450..4797
                     /locus_tag="OO244_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5665422.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRVELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="OO244_003759"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="OO244_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5665423.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="OO244_003760"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="OO244_003760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5665424.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="OO244_003761"
     CDS             6314..6412
                     /locus_tag="OO244_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5665425.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="OO244_003762"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="OO244_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5665426.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..6841)
                     /locus_tag="OO244_003763"
                     /pseudo
     CDS             complement(6650..6841)
                     /locus_tag="OO244_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1672 a         1694 c         1697 g         1882 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgtag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa tacccctagc ttgtttctat ttgctcagat tttaataaca
     6841 actcagcacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc tttgaacccg
     6901 tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaa
//
LOCUS       DAJTOW010000159         6817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTOW010000159 DAJTOW010000000
VERSION     DAJTOW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.159"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..1213
                     /locus_tag="OO244_003764"
     CDS             <1..1213
                     /locus_tag="OO244_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR5665427.1"
                     /translation="VVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKS
                     KYTKEIVTNDSYSHEIINGNGKNILVIFVEGMSSLVISKELTPNLYEFRKKSISFNNY
                     YNHTAATFRGLRGQLTSSYQMTGGYYKDNSGLGQASKVEIEHKLNTYNYITKLPVILE
                     ENGYHTYFQASNSIDAPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNE
                     AKQPFFYGVYTVGTHLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKD
                     TIVIITSDHASYPSEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTP
                     TILDILGIKESHNMFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKIE
                     LIKQLQLFGDR"
     gene            complement(1325..2056)
                     /locus_tag="OO244_003765"
     CDS             complement(1325..2056)
                     /locus_tag="OO244_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR5665428.1"
                     /translation="MRIVGKERAILAKYIQFNQPHAMFWLGFDVDRIGAAIDWSDRNA
                     PAPTLTITNPENGHAHLLYALETSIRTAPDGKMKPLRYAAAVENALRRKLGADTGYSG
                     LICKNPNHSHWKIAADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAY
                     NLQFVSPLSENEVLGISKSISKWTANNFSEDRFIEYVKNTHRSEIQRKRWCKGGKLSN
                     SGGRPKGSFNINSYNFKKRMGVFRC"
     gene            complement(2160..2396)
                     /locus_tag="OO244_003766"
     CDS             complement(2160..2396)
                     /locus_tag="OO244_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5665429.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSSLQRMFETTSQAL
                     ERNGRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
     gene            3698..4015
                     /locus_tag="OO244_003767"
     CDS             3698..4015
                     /locus_tag="OO244_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665430.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEI
                     AKTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKC
                     ADQ"
     gene            complement(4412..4780)
                     /locus_tag="OO244_003768"
     CDS             complement(4412..4780)
                     /locus_tag="OO244_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665431.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYG
                     LNISLVAFDKALYRLRKSTDEPSLAGVDSVSVSETNFSEASGPDNGFFLSGNDFMRAE
                     EESQRVVESFRDSLSRKKGF"
     gene            complement(5164..5313)
                     /locus_tag="OO244_003769"
     CDS             complement(5164..5313)
                     /locus_tag="OO244_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665432.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            complement(5558..6249)
                     /locus_tag="OO244_003770"
                     /pseudo
     CDS             complement(5558..6249)
                     /locus_tag="OO244_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1914 a         1541 c         1469 g         1893 t
ORIGIN      
        1 ggttgttgtt tttttattat cattgttaat accaatatct ccaataaatg cttttttttc
       61 ttcatcatat gaagtttatt cggaagcaaa agaatacaat atgtcaccac atgaaataag
      121 tgatataaag tcaaaataca caaaagaaat agtaacaaac gacagttact cacatgaaat
      181 aataaatgga aacggaaaaa atattttagt aatatttgtt gaaggtatgt cttcattggt
      241 aatatctaag gagctaaccc cgaatttata tgaatttaga aaaaaatcaa tatcatttaa
      301 caactattac aaccataccg cagctacatt tagaggttta agaggacagt taacctcatc
      361 atatcaaatg accggtggtt actataaaga taattctggc cttggccaag catctaaagt
      421 agagatcgaa cataaactaa atacttacaa ttacataaca aaactaccag tcatacttga
      481 ggaaaatggt taccatacat attttcaagc atctaattca atagatgccc cattgagttt
      541 aatgctatca actttaaagt ttaatcatat atacggaagg gaggactata acaaaaagtc
      601 aggtgaactc acagacaaag agtctttcga tttattattt aaaaaattaa atgaggctaa
      661 acaacctttc ttctatggtg tttatactgt aggtacacac cttggcttag atagccctga
      721 tgtaaagttt tatgatggga gaaatgaata tttaaatcac tttcatcaat tagaccattg
      781 gtttggtgat tttatatctc attttaataa gacaagctta tcgaaagata caattgtcat
      841 aatcacaagt gaccatgcaa gttacccatc agaagattac aaaaaaacat tcaaaactga
      901 taatactttt tttgttgatc agattccatt aataatatgg agtaatggtg ttgagttcaa
      961 agacattaat gcacacggtt taaattcact atctttaacg ccaacaatac tagatattct
     1021 tgggatcaag gagtcacaca acatgttcat aggtgagtct ctatttgaaa aagaaaaaaa
     1081 taaaaatgaa ctcaactgta catctgcaat aggtgacctg tatgtgaaaa cgtgtaatgg
     1141 gaaaatggaa atcgagaaaa gaaaagaaaa aatagaatta ataaaacaat tgcaactttt
     1201 tggtgataga taattaaaag gcggttttac cccatgttga ttattagtcc acgtggggtt
     1261 attttaaaat gataataaaa cttacttttt ttcattcttg agccgcctgt agaatgtggc
     1321 tcgactaaca cctaaagact cccattcttt ttttaaagtt ataggaatta atattaaaac
     1381 tacctttagg ccgtcctcct gaattgctta gctttcctcc tttacaccat ctctttcttt
     1441 gaatttctga acggtgcgta ttttttacat actcaataaa acgatcctct gaaaaattat
     1501 tcgctgtcca ttttgagatg ctttttgata ttccaagaac ttcattttca cttaatggtg
     1561 atacgaattg taaattgtag gcacaggctc gttcatagca ggcttgtaac cactgctcat
     1621 actcaggcca cccctgacgg atggcacgat atgcccactt gcgggttttg tcgaacagag
     1681 tgcagttacg gcctaaacca taatcggcgg caattttcca gtgactgtgg ttcggattct
     1741 tgcaaattag ccccgaatat cccgtgtcag ctcccaattt tctacgtagc gcattctcta
     1801 cggcagctgc gtagcgtaac ggtttcattt ttccgtcagg tgctgtgcgt atagaggttt
     1861 ccagcgcata aagcaggtgc gcgtgtccgt tttcggggtt ggtgatggtc agcgtaggtg
     1921 cgggagcgtt gcggtcgctc cagtcaatgg ctgcgccgat gcggtcaaca tcaaatccca
     1981 gccagaacat ggcgtgaggc tgattgaact ggatgtattt cgccagaata gcgcgttctt
     2041 tccctacgat gcgcacacca aagtgcaaat catccgagaa gtacggcttg tgtggtacat
     2101 ggactgacct cgatagcggg ttgcttgctt ctcctgctgt aactgccgtt cctgctgctt
     2161 tacgagcgtg ttaatcggct cagatcgctg ctcaagcgtt cgacctgttc gtgcaaggcg
     2221 ttcacctgat tgctcaagtt cagacatacc ctgccgttgc gctccagtgc ctgtgaggta
     2281 gtctcgaaca tgcgttgcaa gctgctgtaa ctgtcctgcc acgcctgttg ctgttgttca
     2341 taacttgttt gtaagctttc taaggcgctc agaagctgcc tttccatctc ggtcatggtt
     2401 ttgcctgtcc tcagtgataa aaacgtgctg tggcttttct gtgggctctg acggggttat
     2461 ttcgaggtgt acttcatcac ggtgtttccg gctgtcgatg tataggtctc ggtgccatgc
     2521 gctttcttcg gcagtaccac cagataggtg ttcttctgct tgtcgtcctt gtaaatcgct
     2581 acccctgcgc cgctctgggc tttcaggctc tccagttgct ggctgtacgg tcacaccgtt
     2641 caagcagacg ctgatgctca gtctcggtca tgcggatttt gatttctttc gttcgctttt
     2701 ccataggcac cgctgagaaa aaattcgcac cactgtgaga aagtgcttca tgtggcagga
     2761 caaaaaaggc ggcaccagtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     2821 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     2881 ggggcggaga tttcctggaa gatgccagga aaatacttaa cagggaagcg atagagccgc
     2941 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     3001 aaatcagtgg tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggtggc
     3061 tccctcgtgc gctctcctgt tcctgccgtt cggtttaccg gtgtcattcc gctgttatgg
     3121 ccgcgtttgt ctcattccac gcctgacact cagttccggg aaggcagttc gctccaagct
     3181 ggactgtatg cacgaatccc ccgttcagtc cgactgctgc gccttatccg gtaactatcg
     3241 tcttgagtcc aacccggaaa gacacgacaa agcgccactg gcagcagcca ctggtaacag
     3301 gattagcaga gcgagttaat gtaggtggtg ctatagagtt cttgaagtgg tggcctaact
     3361 acggctacac tggaaggaca gtatttggta tctgcgctct gctgaagcca gttaccaggt
     3421 taagcagttc cccaactgac ttaaccttcg aaaaaccacc tccccaggtg gttttttcgt
     3481 tttcagagca agagattacg cgtagaaaaa aaggaactca agaagatcct ttctatgctc
     3541 aaccaccgca tggctcatag ccgcgctggc cgttgccggt cagctccaga atcctcgacc
     3601 aatatggaac cttgcccctc ccaaaattca actaaaaaac cctcatttcc tgccgataaa
     3661 atgggtatct cttaactatg cctaaataag gataattttg aaaaaaatat tcatcctatc
     3721 ggcagcgcta atcatggcag gatgcgcaac aaagcaatat ccacaggccg cgaacgtcac
     3781 cggtccagaa tcttccgcaa tggattgcaa agcggtagct ctggaaatcg ccaaaacgcg
     3841 cagtgtacaa caagaaattg aggaaaccgg cgaatttgac gggcgcaccg tattaggttt
     3901 cctgggtgat tttgggatag gaaatggcat ggcaaaaggc gaagcccgca aaaaagcagc
     3961 taaccggcta aatcagctgg aagccctgaa ggctgcaaag tgtgcagatc aataaggtac
     4021 aaaaaagccc tgcggggctt ttttgtacct gccaatcagc tggatttacc ataacatccg
     4081 ttgcgtgcac cgcctgagag gctctcaggg cgtttctgac gggaaaccgg taaaacaggc
     4141 aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag
     4201 tatgcacgca agtgcatagc gcgaaaattc accccattca gccagggtaa gtcctgtttc
     4261 cggcattctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgagg
     4321 cattgaccaa gccgtaaggc acggtaggta attgcaggta tccgacaatt tcgagtagag
     4381 gatccactat ttcactactt agaattcaaa atcagaatcc tttcttcctg ctaaggctat
     4441 ctctgaatga ctcaaccact ctttgagatt cttcttctgc tcgcatgaag tcattcccgg
     4501 acagaaaaaa accattgtca ggcccactgg cttcagagaa attcgtttct gaaacagaca
     4561 cagaatccac accagcaaga gacggttcat ctgtactttt tctcaatctg taaagagcct
     4621 tatcaaacgc aaccagtgag atattcaggc cgtaacgttc attcagcaca gtcacaatct
     4681 cttccctacg gataccattt ttcttccagg tttctatatc gttcttggaa tccctgacag
     4741 ccgcaatggc tgttcttgga ccagggttaa aattcgccat ataccgccca taatgtctta
     4801 aatttgtctc atgatagtcc aaaaataaga ttgaaacgca aaaaatgtct tacaattgtc
     4861 ttattgttgt cctttccggt tcatgtatcc atgaaccgcc tgatgtggaa cgctttttat
     4921 cgatctcatg cgcagccaaa gatagattca ttctttaaaa gtcaggttac attatccctt
     4981 aaatggtcat acaccatacg caaccgcgat tttcatcgca attgcatgaa ttaaaaggta
     5041 aaaactgaag tccccgttta tttatgtaat acgcaacgta ttacaaccac ctgtaattac
     5101 atgaaaataa agaaataaga tgtaataccg gcgtattacc acgtacgcgg gtaagcgaaa
     5161 aagctataac gcttcccggc cgacaggcgg cccaaaatcc accggggcgt gcaggttctc
     5221 ttcagtaacc tgcgccatta aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt
     5281 cgaatccagt tgttcatcat gttcagatgg catggggtga tatccagtag ctgagaggtg
     5341 gttagcgggt ttcggggcgc agccctaaac cagtcacgta gcgctagcgg agtgtatact
     5401 gacttaggcg gtagtgtagt atgacagatt ctgtggttac ggcaaagtaa cagccctgtc
     5461 taggagaggg ctgttatctg atataggctt gtttttttct caccgttttt gcgagcgctt
     5521 tttcctgcca agaggtaggt aggataatga atccaactta ctgatagtgt tttatgttta
     5581 gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac agtgacttcc
     5641 gtcccggcct tgccagatgc tgcctcagat tatgccgctc aattcgctgc gtatatcgct
     5701 tgctgataac gtgcagcttt tccttcaggc gggattcata aagcggccat ccgtccgtca
     5761 tcaataccac cacgtcaaag gccgatagca ggctcagaag acgctccagc gtgaacattg
     5821 tgcgttcacc aaaaacgtgg gccacgacag tcctgcgtac cctgtcatac gcgtaaaaca
     5881 gccagcgctg gcgtgattta gccccgacgt agctccactg ttcgtccatt tccgcgcaga
     5941 cgatgacgtc cctgcccggc tgtatgcgcg aggttaccga ctgcggcctg agttttttaa
     6001 atggcggaaa atcgtgttga ggccaacgcc aataaagcgg gcggttgccc ggcatccgac
     6061 gccattcatg gccatatcaa tgattttctg gtgtgtaccg ggttgagagg cggtgtaagt
     6121 gaactgtagc tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcagt
     6181 acttttaccg ttacgcacaa cgccgtcagt agcggaacag gagggacagc tgatggaaat
     6241 ggaagccacc ggagcacctc aaaagcaccg tcatccacta aatcagtaag ttggaagcat
     6301 caccgcacta gcagacagtt aattaaaaaa gtataatcca tctccagatt tgttgaataa
     6361 tggagaatta tgctgttttc tatgtcttat ttagtcaaat gcgcttatca atattgtgat
     6421 aagctcccga gtctaaactt aacttactga ttttaggttg aggaagaccg acatttttgt
     6481 agatccacag cataatatct atttattcaa caacgccccc caaaaaaaaa ggtttttata
     6541 aatgttttta atttcaatta ttcaaatgtt ctcggttatg attataatgc ttaatataaa
     6601 aataaaagat gcatttagag caattattct gcttttactt ctattactat ccatggttat
     6661 atctattgaa ataagcagca ttatatcaat aggtgagtac atacccacac ttgcactagc
     6721 aaatatatca gagacaaata gtgttggctg gaaaataaaa ctactacctt ttcttgtgtt
     6781 cttatcattt atgattgcat cattgatttt gaaaaga
//
LOCUS       DAJTOW010000160         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTOW010000160 DAJTOW010000000
VERSION     DAJTOW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.160"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            240..2672
                     /locus_tag="OO244_003771"
     CDS             240..2672
                     /locus_tag="OO244_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5665433.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO244_003772"
     CDS             2739..4208
                     /locus_tag="OO244_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5665434.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO244_003773"
     CDS             4208..5869
                     /locus_tag="OO244_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5665435.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO244_003774"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO244_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5665436.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO244_003775"
     CDS             complement(6478..>6795)
                     /locus_tag="OO244_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665437.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1613 c         1553 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaccccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccaaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTOW010000161         6772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTOW010000161 DAJTOW010000000
VERSION     DAJTOW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.161"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..162
                     /locus_tag="OO244_003776"
     CDS             <1..162
                     /locus_tag="OO244_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5665438.1"
                     /translation="QEMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQS
                     RNKEGTNDV"
     gene            512..874
                     /locus_tag="OO244_003777"
     CDS             512..874
                     /locus_tag="OO244_003777"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR5665439.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            871..1788
                     /locus_tag="OO244_003778"
     CDS             871..1788
                     /locus_tag="OO244_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5665440.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1801..3066
                     /locus_tag="OO244_003779"
     CDS             1801..3066
                     /locus_tag="OO244_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5665441.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3133..3756)
                     /locus_tag="OO244_003780"
     CDS             complement(3133..3756)
                     /locus_tag="OO244_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665442.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTAILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4474..4845)
                     /locus_tag="OO244_003781"
                     /pseudo
     CDS             complement(4474..4845)
                     /locus_tag="OO244_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            4967..5227
                     /locus_tag="OO244_003782"
     CDS             4967..5227
                     /locus_tag="OO244_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR5665443.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5245..>6772)
                     /locus_tag="OO244_003783"
     CDS             complement(5245..>6772)
                     /locus_tag="OO244_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665444.1"
                     /translation="PATRNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLN
                     VDSLLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDA
                     LGMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGM
                     KNLDPIEKRVFRAVNPGKNGALTYANLNKQRRLVGDALHKNSGPYKDADRAALSRLYG
                     SLADDQKAALSETNALRDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQA
                     MSKGDAKGFRELMQNTPSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLAN
                     GQMRNLARHLPKETMSGLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFV
                     ANHAQRIGTAVGSTVSGPFSAVGAVAGSEIGTKVASKIRAMGGAESIESAEKLISSPE
                     FQKAARLAVKQAPESIVDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK
                     "
BASE COUNT         1883 a         1479 c         1362 g         2048 t
ORIGIN      
        1 caggaaatgg gaggctggga gtctatcgaa atggttcgtc gatatgctca cctcgcacct
       61 aatcacctta ccgaacacgc acggcaaata gacttgattc tgaacccatc ggtcccaaat
      121 ttgtcccagt caagaaataa ggaaggtact aatgatgtgt aacttattga tttaaatggt
      181 gccgataata ggagtcgaac ctacgacctt cgcattacga atctgtagca ccaatcataa
      241 ctatctgttt tagcaagcat taaccgcatt cactaagata tagttgatgg cacaaacaga
      301 aagttgatga atgatgttgc catatatatg tcacaaatac ggcacaacga tcttcaaaca
      361 tatagccaca cattcacaga agagcacaaa gccttgcaat ccagtgcaaa gatttgtgtg
      421 ccagagtttt tctcatcaac taccgcaagt atcgatcgat tgagacttgg atgatagact
      481 tcatgccttt gattattagc tgatagaaga aatgttaaag ctatttgcaa agtacacatc
      541 tataggcgtg ctgaacacac ttatacactg ggtggttttt ggtgtatgta tctatgccgc
      601 gcataccaat caggctatgg caaacttcgc aggtttcgtt gtggctgtga gttttagctt
      661 ctttgcgaat gcaaaattca cattcaaagc atcaactaca acgatgcgct acatgttata
      721 tgtcgggttc atgggaacac tgagtgctac tgttggatgg gttgctgata gatgttcact
      781 tcctccaatt gtcactcttg ttaccttctc cgccatcagc ttggtgtgcg gtttcgtcta
      841 ttcaaagttc attgtcttta gggatgcgaa atgaagatat ctcttgtcgt tcctgtcttc
      901 aatgaagaag aagcgatacc aattttttat aaaacggtac gtgaattcga agagttgaag
      961 ccatatgaag tagaaattgt tttcataaat gacggaagca aagacgctac ggagtcaatc
     1021 attaacgctc tggctgtttc tgatccgcta gttgttccgc tgtcatttac acgcaacttt
     1081 ggtaaagaac cagcattgtt tgcagggtta gaccatacaa ccggcgatgc cgtgattcca
     1141 attgatgttg acctgcaaga cccgattgag gttattcctc atcttattga aaagtggcag
     1201 gcaggtgctg acatggtgct tgctaaacgt tcagaccgct caactgatgg acggctgaaa
     1261 cgtaagacag ctgagtggtt ctataaatta cacaacaaaa taagcacccc aaaaatagag
     1321 gaaaatgtcg gagattttcg actcatgtct cgtgaggttg tggagaacat taaactattg
     1381 cctgagcgca atcttttcat gaaaggcata ctgagctggg ttggtggtca gacagatgtc
     1441 gttgaatatg tacgcacaga gcgtgttgct ggcatctcaa aatttaatgg ctggaaatta
     1501 tggaatctgg cactggaagg tatcacaagt ttttcaacct ttcctcttcg cgtatggact
     1561 tatataggct tgtttgttgc aagcatttca tttttatatg gtgcatggat gattatagac
     1621 accattgtct ttggtaaccc agtacgcggg tatccctcca tgcttgtatc aatacttttc
     1681 ctgggtggag tgcaactgat cgggattggt gttctcggtg aatatatagg tagaatttat
     1741 ttagaaacaa aatcaagacc acggtactta attaaaagca gaaaataaca ggatgatctt
     1801 atgaagatat tcataatgcg caattggcac aagatatcaa tttttatact tgcatttact
     1861 ttgatttggc ttaggcgcat tgacattttg acaaatgccc agttttgggc tgaggatgca
     1921 gttttttggt ataaagatgc atacgaacaa ggttttttca gttcactcat aaccccaaga
     1981 aatggttatt ttcaaacagt atcaacactc attgttggtg cgacgacatt tattaatcca
     2041 atttacgcac cattattatc aaactttttt ggaataataa ttagagctat aattatatgg
     2101 ttcttattta ccgatagatt taaattccta agcaccacat caaaaatctt catctctgta
     2161 tatttaattt gcatgcccgg gcttgatgaa gtccaagcca atataacaaa tgctcactgg
     2221 tatttatcat tgtatgttgc aatgataata atatctgaag aatccaagtc aaaattatgg
     2281 aaagcacatg atttattttt tatagtatta tctggattga gcggtccatt tattatattc
     2341 ataattgcat catctgtttt taaatattta cacttatcaa aaggagtaat aagcataaga
     2401 ggtttatact tattttatac tcgactacca tatttggcaa tgatcttgtg tgggctgata
     2461 caggcaacat caataattct tacatttaat ggaacaagat cacatgctcc attaggattc
     2521 agttttgatg ttatgtcatc tataatatca tctaatgtat ttcttttttc atttgtccct
     2581 tggaatatcg ctaatgctgg atgggataat caccttttat cgtacacact atcaacttta
     2641 gtaattacat tatgtgtttt catttatatt aaaggaaatt ggcaaatgaa agtgtttgcc
     2701 acccttccaa tacttattgt cgcattttct atggcaaaac ctcaactggc agattctgtt
     2761 cctcaactgc caacacttgc aaccggaaac gggtcaaggt attttgtaaa tattcatatt
     2821 gcaatttttt cattaatatg cgtttgtttg tttcaatgca taaagaataa aaccttaaaa
     2881 atattcttta aaatctatgt ttcagtcatc cttattgcaa tgataaaatt aaactttttt
     2941 ataacccctc ttcctgacat gaactggtct caaggagcag aattaataaa taaagcaaaa
     3001 catggagaag ccgtctcatt tacagtgctc cctccatggt taactcttga tttaataaaa
     3061 aaataaaact tcacattaat aaataacaat gaatccgcct ccctacgtat tcccgtaggg
     3121 aggctgtcgt gtttatagta gcctggtaat gctaaatgca aacgaactgg agattcctgc
     3181 tccgctaagc gtattgcgca gggtcaccct aaatgtatct acgccaatat aagtaacatc
     3241 acaactaact gaatcaggcg cagatatagg tgttatctgc acaatataac cgctagcaac
     3301 tgctgcgagc ggatgagaaa agctaacctc atacacacct gtagcaacca cgttaaccga
     3361 tgaaacacca gtagcaacgt cgatactacc attggcgtta atttttcccg agcgtgtgac
     3421 tgacaatgca gaagccgatg ttgccttact ggttgtttct gtaaaaacac acccaatgtc
     3481 aactacctcg caattagctc ctgttatcca gtatggtcga tctgcacttg gctgataatc
     3541 ccctgcgctc tggaacgttg tgccgttgag tatggctgta acttttcccc cacctgagct
     3601 tgttacttga atattagtgt gcgtgtatct tgcactgctc acgcgatgaa aatttccacc
     3661 gtttataacc acagttacag ggcgcgtccc agtattatct atagcaaggt ctgccccccc
     3721 ggcattagcc tcaaagtatg ggttgtttaa taccaaagcg cacgaaccgt taaggccatt
     3781 tatggctaat tgcgcaccac ctgtactgga atccccctga gtaccatttc cttcacaagt
     3841 caggctattg atagttgtac cagcacccca gcggtccccg aggatacccc attcactgtt
     3901 ctgactgcac gtaagcctat ttgcagttat agcgttcagt tcagaatgat ttacggagtt
     3961 ggtagcacgc caaccgatac gattactctg caagtacacg ttcgtcatgt cgctcgtgag
     4021 catcgagtca gtgcgtaacc cttccccgtc agggtggcgg cagataacaa tgttttcaag
     4081 acgagtatgt gctttattct gcaccagcat accgtaactt ccgggattcc ctttcagcgt
     4141 catgttgccg atgtagtcat atgcagccaa tctctgactg ttaaacgctg cgtcacctag
     4201 catttttacg ttaaaatcag tagctctaca ttccagtatt gtctcagcca tgttctcgcc
     4261 tgagtaaaaa cagcggacac ccggagcacc tacaaagtcg gcagggtaat catcataatt
     4321 aagtaccaga ggagtggtaa catcatacac cccttgcttt atttttaatg ttccaccgaa
     4381 aggccacgat gtaagcgggt cttgatatat ttcatttttt attgcaaata gctcatcttg
     4441 aagatttttc ccgctattag tgccaatgta agtagcccct tcatcagatt gaagtaactg
     4501 cagaagctgt tcagggtcat acttcagcaa attcggaaaa tagaactgtt gcgaaccata
     4561 cgcatcatat acagccattg aatggccttg ctcagttaca aatttggcaa tctgcccgtt
     4621 gtacacagga tatccggcag cattgataac aattggctga gaaacaggga cgtgagatcc
     4681 gtcttcattc tctacataaa cctgaatctg gttttcagta tttaccgggt cagtgtcaat
     4741 ttttccgata taaattttgc cattggctac agctttaaaa gaacgcgcca tagtgaagag
     4801 ttgcgaaggc atgcttacca caacatttgc ggtgatatct gacatttcat tgctccagac
     4861 gaatgatatg atgcaatcat gatatgattg cataccgaaa tggtactatt gagtatttat
     4921 ccagtaggtt acgatgccat tccacccaac tggtgaggca tcaaggatgt acagcaaata
     4981 cgacgaggcg cagtttcact tgagacttcc gcatgaactc cacgcgaaaa ttaaacagcg
     5041 cgcgaagatg aataacaggt cgctgaactc agagataatt gcagcgattg aagaatcatt
     5101 ggctaaacaa agctctgcat cagtttacat tgacgatgca gagcgtatgg cagaacaaca
     5161 atctgatatg gttaagaaaa ttgtctttga tacgctcaag gagctatata aaaaagacag
     5221 caactaacca tccgtggctt tccgttactt ctggttatca tcactgttca tccagtacat
     5281 gatgcctagc cttgatatgg ttttcttatc tgattctgga agtgagttgt aaaacgagcg
     5341 ccaagcagaa gagcgtctta cagttgtatc aacgatgctt tctggtgctt gttttactgc
     5401 cagccttgct gctttctgga attctggtga gctaattagc ttttctgcag attcaattga
     5461 ttcagcgccg cccatcgccc tgattttgct ggcgactttt gtcccaattt ctgacccagc
     5521 aacagcacct actgcactga acggtcctga cacagttgaa ccaaccgcag tgccaatgcg
     5581 ttgggcatgg ttagcaacaa attcatttgc cgcagtgacg cgattaaacc gtttgacgaa
     5641 ctcgtttagt cttcctgtag ttatctcgta agattttgcg tcttttatag cctttgcgac
     5701 cttatataca tcgttcagac ctgacatagt ctcttttggt aaatgtcggg caagattgcg
     5761 catctgcccg tttgctaaca tgttttgata ccagtcagca aaccctgcag gattaaaatc
     5821 agcgccacgt tttccgttcg aaagcatatc ccgaagacct gttcccagta gctcggttct
     5881 tagcttcctg gacggcgtgt tttgcatcaa ttcacgaaat cctttggcat cgcctttcga
     5941 cattgcctgt agtgctgtag ttgctttgcg agaaacatca ccgttcagcg ttctgccagt
     6001 tagattaatc atttgctctt ccatgctttt tcgcatctga acaagcctct gagcaacttc
     6061 aaaatcacgt aatgcatttg tctctgacag cgccgccttt tgatcatcgg cgagcgaacc
     6121 gtaaagcctc gataaagcag cgcgatcagc atctttatat ggtccagaat tcttatgaag
     6181 tgcatcacca acaagtcgtc gttgcttatt gagatttgca taagttaatg cgccgttctt
     6241 gcctggatta actgcccgaa atactctctt ttcaataggg tcaaggtttt tcatcccgcc
     6301 aagatcttct gccacctgtt tgagcattga ccttgttgat ggcgcatcaa tttttgcacc
     6361 tgcaggcata gccttttcaa cattgcgata aagctggtcg cttcgtttca tcagcgcaga
     6421 cattcttcca ttgaccgtat caataaactt gtcgctcata ccaagagcat cttttgcacc
     6481 ggaaacgcga tcaattatct cccccgcgcc ctgtgctagc tgcctgattg cttcattttc
     6541 ctgcacctgt agagcagaac ccgcacggga cttgattgct tgctcaactg ccttgtattg
     6601 ctcgttcccg gaaaagtgtg aagggagtaa tgaatcaaca ttaagccttt cagcagactc
     6661 cagaacttca gcctgaggat tgatatctag ttcatcaagc gatgaagcga gatttggcct
     6721 tttttgtgct gctactgtgc gaacgacctc ctctgggttc ctcgtcgcag gc
//
LOCUS       DAJTOW010000162         6731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTOW010000162 DAJTOW010000000
VERSION     DAJTOW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.162"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..483
                     /locus_tag="OO244_003784"
     CDS             <1..483
                     /locus_tag="OO244_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5665445.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
                     "
     gene            853..1677
                     /gene="gadX"
                     /locus_tag="OO244_003785"
     CDS             853..1677
                     /gene="gadX"
                     /locus_tag="OO244_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5665446.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            2045..2773
                     /gene="gadW"
                     /locus_tag="OO244_003786"
     CDS             2045..2773
                     /gene="gadW"
                     /locus_tag="OO244_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5665447.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHCKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3136..6249)
                     /gene="mdtF"
                     /locus_tag="OO244_003787"
                     /pseudo
     CDS             complement(3136..6249)
                     /gene="mdtF"
                     /locus_tag="OO244_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(6274..6531)
                     /locus_tag="OO244_003788"
                     /pseudo
     CDS             complement(6274..6531)
                     /locus_tag="OO244_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1764 a         1589 c         1605 g         1773 t
ORIGIN      
        1 ggtggtcaaa ttggtacttt tgccatcaac ttctcccgcc cggcgggtca ggtaattgca
       61 cagtactatg aattcctgcg cctcggtcgt gaaggctata ccaaagtaca gaacgcctct
      121 taccaggttg ccgcttatct ggcggatgaa atcgccaaac aggggccgta tgagttcatc
      181 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      241 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      301 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      361 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      421 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacacacc
      481 tgataacata acgttgtaaa aaccgaatgc ccagccttta aaaaaacagc tgggcattcg
      541 gttgcttatt aatcgcaata atatattggc tgtctattca tcgtgttgat aagatatatg
      601 ttatatgaat gtttattaaa tgtagcgatt acctttaatg tcacgaaaag ggattatttg
      661 cttgctatta atttcactgc gttcttaaac acgtttttgt attataaatc cagtcatcct
      721 gcccgtagaa catcacacat tatcatcctg ttctcccgct tcgtttaaat ttatttatca
      781 atcaatttga cttaagaggg cggcgtgcta cattaattaa cagtaatatg tttatgtaat
      841 attaagtcaa ctatgcaatc attacatggg aattgtctaa ttgcgtacgc aagacataaa
      901 tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga cctggttttt
      961 gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt agtatcaagg
     1021 gatacgcttt cattatttct gccgatgctc aaggaggagg cattaaatct tcatgcacat
     1081 aaaaaaattt cttcattact cgttcatcac tgtagcagag atattcctgt ttttcaggaa
     1141 gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagagca
     1201 ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc gctgcttttg
     1261 aacgttttgc aaccgaacat gcgaacacga gtttgtacgg ttatcaataa taatatcgcc
     1321 catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag cctgttaaag
     1381 aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg tagaatgcaa
     1441 cgtgctttgc aacttattgt tatacatggc ttttcaatta agcgagtcgc agtgtcctgt
     1501 ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg gatgacgccc
     1561 acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc ggcaagtatt
     1621 gttgcgcaag ggaattttta cggcactaac cgttctgcgg aaggaataag attatagagt
     1681 tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct
     1741 ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta
     1801 agttaaatat aacttttaca ggaaataaga tcagccattt ttttataaac ataagctata
     1861 cgctgtgcga aaatatattc gtgctgcatt tacttattat caattaactg ttatgcaaaa
     1921 ctactttgtg gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt
     1981 ctgtcatcat ttcattagta tactgaaatt gaaataatcg cagtatgaaa tataagggat
     2041 aatcatgact catgtctgct cggtgatcct cattcgtcgt tcattcgata tttatcatga
     2101 acagcaaaaa atatcgctgc ataacgagag tatcctgctg ctggagaaaa atttggcaga
     2161 cgattttgcg ttttgttcac cggatacgcg acgactggat atcgatgagc tgacagtttg
     2221 ccattactta caaaatattc gtcagctacc acgcaattta gggttacatt gcaaagaccg
     2281 tctgttaatt aaccagtcac cccccatgcc gctggtgacg gcgatttttg atagcttcaa
     2341 tgaatccggg gtaaattcac cgatactgag caatatgctc tacctttcct gtttatcgat
     2401 gttttctcat aagaaagaac tgatcccctt acttttcaat agcatcagca ctgtttcagg
     2461 aaaagttgaa cgccttatta gctttgatat cgccaaacgt tggtatctgc gcgatatcgc
     2521 ggaaagaatg tataccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg
     2581 tttcagtaaa atattactcg cttccaggat gtcgatggcc agacgattac tcgagttacg
     2641 tcaaattcct ctgcatacta ttgcggaaaa atgtggctat agcagtacgt cgtactttat
     2701 aaacacattt cgacaatatt atggtgtaac gccacatcag tttgcgcaac attcgccagg
     2761 taccttttcc tgacatattt tgcatttgaa tattggtcag gatctcacac ctgcttcatg
     2821 tgaaactctt ccctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc
     2881 aggcggtata ggtattttta ggaggaaaag actcggcatg tttgggatta ttaagctgac
     2941 aattcatacg attacgggaa tgtgggtcag tattgtgttg ttcaaattga tgacgaatgg
     3001 ctggagtggt ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg
     3061 gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct gaaccttcac gttcaacctt
     3121 actctcattt acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa
     3181 aaagaccgga acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccatcacgcc
     3241 ggtacctacc gcgttttgcg cgccagaacc ggcaccatga ctgataacca gcggcagcac
     3301 gccgagaata aaggccagag aggtcatcag gattgggcgt aaacgcatcc gcgccgcttc
     3361 gatgattgcc tctatcggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac
     3421 aatcaggatg gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta
     3481 gacgtcatta cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg
     3541 aacaaccaac atcaccgaga acggaattga ccagctctca tagagtgcgg cgagggcgag
     3601 gaacaccacg accagtgaaa tggcatacag tgcaggagcc tggtttgagg ataacgcttc
     3661 ctgatacgac agtccggtcc atgagtagcc gacgcctgcc ggaagtttag cgaccaggtc
     3721 tgccataaat ttcatagcgt caccggtact tttcccggcc gccgcttcac ctaaaatctc
     3781 cattgacggg atgccgttgt agcgttccaa tcgcggtgaa ccataggtcc attcggtaga
     3841 cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat
     3901 gttatccggc aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc
     3961 ctggttgagg aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat
     4021 atcagacagc gccacaccca ttgcctcagc tttcgcggcg ttgacgttca ctttgaacat
     4081 cggcgtatct tccaggccgt tcgggcgtac cccggtgacc tggtcaggcg attgcgctgc
     4141 cagtgataac agctcgtttc gtgcctaggt tagtttttcg tgccccaggt taccgttgtc
     4201 cagcagttcc atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa
     4261 cgggaagacg acggctttat tgatactgct taacgcaatc attgcccgct gaatgatcgc
     4321 ggtaaccgag ttttcctcac cgacacgttc agaccacggc ttgagactga taaacgccag
     4381 gccgttgttt tgcccctggc cgctgaagcc aaagccgcca acggtaaaca ccgactgaac
     4441 attatctttc tcttttgtaa gataataatc cgtcacctgt tgcagtactt tcgtggtgtt
     4501 aaccatggtg gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc
     4561 tggtaagaaa gaggtcggtg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag
     4621 gtagaccacc atgtagcgac cggtacaacg caacagcgag cgggtgctat cggtatagtg
     4681 ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgacc
     4741 gccttccggc gtggctttca gaatggtggc gcacagggca ggggtcaggc tcattgccac
     4801 aaatactgaa agccgcatgg aggagatcag cgtgatggag aactggcggt agatctcccc
     4861 ggttgcaccg ctcataaagg ccatcggcat aaacactgca gaaagaacaa cggcaatccc
     4921 gaccagcgca cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt
     4981 atcttccgca atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa
     5041 ccctatcgcc agcaccatcc cgaacatagt caacgtgttg atggtgaaac cgaccgccga
     5101 caagatcgca aacgtcccga gaataaccac cggtacggca attgtcggga tgatggtggc
     5161 acggaaattc tgcaaaaaca gataaatgac caggaagacg aggatgatag cctcaaccag
     5221 tgttttgaaa acttcctgaa tagaaatttc gataaacggc gtggtgtcgt aaggataaac
     5281 cgtcttcaga cttgcgggga aataggctga taagcggttc agctcctctt tgactgcccg
     5341 cgaggtatcc agggcgtttg ctccggcagc cagtttgatg gcgatcccgg cagcaggttt
     5401 gccgttatag cgcgccacgg tggaataatc ttccgcccca agttcgacgc gggcgacatc
     5461 acgcagcagc acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc
     5521 cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt agttgctggt ctgccgcctg
     5581 tggcatgcca cccagttgac caccagaaat ctggttgttt tgcaccttaa tctgggaaat
     5641 aacatcggaa ggtaccaggt tatatttatt gagtttttgc gggtccagcc agatacgcat
     5701 ggcgtactcg gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc
     5761 tttgatatta gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga
     5821 aataaacgcc gctaccatca ggatattact gctcgactta tcgacgctaa tcccctgttg
     5881 ctgcaccgct tcaggtaatg aaggcatagc gagttgcagt ttattttgca cctgaacctg
     5941 tgcgatatca ggagatgccc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc
     6001 atcactggtt gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac
     6061 ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc ggataggtag cgctgacggt
     6121 aatggtcggt ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc
     6181 acctgcaagc atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata
     6241 gttagccatg aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt
     6301 ttcctggctg gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga
     6361 aacgatcacc cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc
     6421 tttgctggct tcaatttcgc gtagctgcac gacatcgtct ttatccagaa tgagcgccgt
     6481 tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc accagtaata taaagcgcgc
     6541 aaaattattc aagatatttt tgaaaatcat tcaagatagg ctatcgcgtg tgatagcaaa
     6601 aacattcaag ataaaaatgc ttgattatta agcaatcagg aatgggagta ttaagtttcg
     6661 tgtttccagt aaaaccagag agtattgaaa tagctatcac ctaactttat caggcgatag
     6721 ctggtagatg a
//
LOCUS       DAJTOW010000163         6520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTOW010000163 DAJTOW010000000
VERSION     DAJTOW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.163"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO244_003789"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO244_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5665448.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO244_003790"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO244_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="OO244_003791"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="OO244_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5665449.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1556 a         1713 c         1692 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg cttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac
     4081 gagtatatgc agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga gcatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gtaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcaaattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAJTOW010000164         6269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTOW010000164 DAJTOW010000000
VERSION     DAJTOW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.164"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..667)
                     /locus_tag="OO244_003792"
     CDS             complement(<1..667)
                     /locus_tag="OO244_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR5665450.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(657..2414)
                     /locus_tag="OO244_003793"
     CDS             complement(657..2414)
                     /locus_tag="OO244_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5665451.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2430..3314)
                     /locus_tag="OO244_003794"
     CDS             complement(2430..3314)
                     /locus_tag="OO244_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5665452.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3325..3930)
                     /locus_tag="OO244_003795"
     CDS             complement(3325..3930)
                     /locus_tag="OO244_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5665453.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(4101..>6269)
                     /locus_tag="OO244_003796"
     CDS             complement(4101..>6269)
                     /locus_tag="OO244_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5665454.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1834 a         1566 c         1318 g         1551 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
      241 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
      301 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
      361 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
      421 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
      481 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
      541 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
      601 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cgacgttcag
      661 ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc acacatcaat ccgttgagtg
      721 catttatcaa atccggcatt acgcacgagc gaatccatct ccccttgcga acgcacgcgc
      781 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt
      841 gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc attggctaga
      901 cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatatag gccagagaca
      961 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc
     1021 tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
     1081 acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
     1141 gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
     1201 ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt tttgcgctgg
     1261 cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg cccgaatgcg
     1321 ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga atcaaagccg
     1381 gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat cgcctttcgc
     1441 ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga aaggagccgc
     1501 catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt ttgcgatttg
     1561 ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc cctgttctct
     1621 tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     1681 aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc acctgatatc
     1741 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     1801 tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa
     1861 ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga attaataaaa
     1921 aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     1981 ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc ataatcatga
     2041 acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac ctcttccagt
     2101 ccgacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgagcgc
     2161 ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg ggcatcccag
     2221 gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag acgaccagaa
     2281 tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagtgggc
     2341 cagtgccgat aatacaatgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     2401 cgtgaatttt ccattttctc tattttcttt cagtaacagc agtagcgaat aaaataaaaa
     2461 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctagt
     2521 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
     2581 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
     2641 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccagc
     2701 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     2761 ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag aaataacacc
     2821 agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc ggcatgactc
     2881 aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg ctgaaaaccg
     2941 gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt caaaggtata
     3001 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
     3061 caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     3121 aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat caccagtgaa
     3181 aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc cccttttctc
     3241 tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac aacgcgaaaa
     3301 gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca cactccgaca
     3361 acgattaatc gcagtacaga taagcagaat tgaagcaatg ctccataaaa gattattcca
     3421 ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata ttaacgcgcg
     3481 atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct gcgcgagtaa
     3541 accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg atgcgttact
     3601 ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat cgccagtctc
     3661 attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca tgccatccag
     3721 cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga ggatgggttg
     3781 tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg taagagtgat
     3841 gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta acagcgactg
     3901 aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg gtttgcgcta
     3961 tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa aaattcagaa
     4021 tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa tcaagtagtt
     4081 aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc ttgttcccgt
     4141 attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc agggttaaca
     4201 agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc aactggaaag
     4261 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtgccgca
     4321 gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc
     4381 agtgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
     4441 tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg ggagctattt
     4501 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc
     4561 ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
     4621 tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac ttcgccgtgg
     4681 ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga atctgctttc
     4741 ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat cgacgtgctc
     4801 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
     4861 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa gtaggcaggg
     4921 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa tgctaaaaat
     4981 aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc tgcaactttg
     5041 tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc tgccagcttg
     5101 aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat tgcctgattg
     5161 taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
     5221 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
     5281 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
     5341 agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat ttcgacagag
     5401 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
     5461 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgttcatca
     5521 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
     5581 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
     5641 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
     5701 tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
     5761 tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg atttattatc
     5821 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
     5881 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac cccacgcgca
     5941 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
     6001 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
     6061 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
     6121 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
     6181 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
     6241 ctgttgatag cttgttcaac ttgttgttt
//
LOCUS       DAJTOW010000165         6016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTOW010000165 DAJTOW010000000
VERSION     DAJTOW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.165"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="OO244_003797"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="OO244_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5665455.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="OO244_003798"
     CDS             580..1029
                     /locus_tag="OO244_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5665456.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISEYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /locus_tag="OO244_003799"
     CDS             complement(1030..1263)
                     /locus_tag="OO244_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5665457.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO244_003800"
                     /pseudo
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO244_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="OO244_003801"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="OO244_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5665458.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2214..3023)
                     /locus_tag="OO244_003802"
     CDS             complement(2214..3023)
                     /locus_tag="OO244_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5665459.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO244_003803"
     CDS             complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO244_003803"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5665460.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4679..5473)
                     /locus_tag="OO244_003804"
     CDS             complement(4679..5473)
                     /locus_tag="OO244_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5665461.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5463..5810)
                     /locus_tag="OO244_003805"
                     /pseudo
     CDS             complement(5463..5810)
                     /locus_tag="OO244_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1564 a         1530 c         1347 g         1575 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcgaat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg ctagagagac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaat atttccattt
     2281 gatatcaatc gattcatctc ttcaacaaaa taatttatat catcacactt attattatcc
     2341 ggaagatctg agtagttata attccaccat tttattctca acagttcatc aataacattt
     2401 gaatcaaacc taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc
     2461 acatctttgg taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca
     2521 cctttcaata caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt
     2581 ttggtaatga atggtttcaa tgtccagcta cgttcaaatt cactcatagc aaacttatca
     2641 aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttccaccc
     2701 attattttta catttgatgc aatactacaa aatcgaccta tacgtatatt ccctgaaaaa
     2761 tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg cattgttgaa
     2821 tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat
     2881 aaattccttt ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata
     2941 caaaactcaa agtgtttttt cgtccatctg aaggtaaggg ttttattttt atcttccgtg
     3001 aatttgtaca attgaacttt cattgttaat acttctactt ataaagtatt agtttaataa
     3061 cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat
     3121 ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa
     3181 ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt
     3241 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     3301 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     3361 tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt
     3421 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt
     3481 cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
     3541 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
     3601 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
     3661 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
     3721 ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc agcgtatcgt
     3781 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca gcattgtaat
     3841 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
     3901 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg gtatggctga
     3961 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
     4021 cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc acgcctgccg
     4081 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     4141 gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac gcggccatca
     4201 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
     4261 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
     4321 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
     4381 gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt tcttcgataa
     4441 catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc ccccattcag
     4501 caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat acctcggcaa
     4561 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     4621 taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt atcagtggtt
     4681 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     4741 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     4801 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
     4861 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
     4921 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
     4981 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
     5041 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
     5101 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
     5161 ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg gcagctgcca
     5221 gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt gacagcgtca
     5281 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
     5341 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
     5401 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
     5461 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtacag tgcgatgaga
     5521 ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
     5581 gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa
     5641 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
     5701 ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc cgacgcctgc
     5761 aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggatcat cgagtaggca
     5821 gcctgacggc tgcggcttgt catggtctgc aattaccgtt ataaaaacag gcaatgtcat
     5881 tgtctttcag gtggttatat ggcccgttca gctaaacccc gtaaacgtaa acccgcctct
     5941 caaagttcct gagtcgtgac ccgttcgaat gcgtgctgtg tggcggccgg atggtatacc
     6001 gccgggccat tgcggg
//
LOCUS       DAJTOW010000166         5657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTOW010000166 DAJTOW010000000
VERSION     DAJTOW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.166"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..378
                     /locus_tag="OO244_003806"
     CDS             <1..378
                     /locus_tag="OO244_003806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665462.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVK"
     gene            complement(454..918)
                     /locus_tag="OO244_003807"
     CDS             complement(454..918)
                     /locus_tag="OO244_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5665463.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO244_003808"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO244_003808"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5665464.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAVADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO244_003809"
     CDS             complement(1787..2476)
                     /locus_tag="OO244_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5665465.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO244_003810"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO244_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5665466.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO244_003811"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO244_003811"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5665467.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..>5657)
                     /gene="nanR"
                     /locus_tag="OO244_003812"
     CDS             complement(5139..>5657)
                     /gene="nanR"
                     /locus_tag="OO244_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5665468.1"
                     /translation="RARVSRPSADTIIGELSGMAKDFLSHPGGIAHFEQLRLFFESSL
                     VRYAAEHATDEQIDLLAKALEINSQSLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHV
                     ALLDWLIAARPTVTDQALHEHNNVSYQQHIAIVDAIRRHDPDEADRALQSHLNSVSAT
                     WHAFGQTTNKKK"
BASE COUNT         1411 a         1562 c         1442 g         1242 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaagtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgacagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcg
//
LOCUS       DAJTOW010000167         5547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTOW010000167 DAJTOW010000000
VERSION     DAJTOW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.168"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..631
                     /locus_tag="OO244_003813"
     CDS             <1..631
                     /locus_tag="OO244_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5665469.1"
                     /translation="CIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITTGKD
                     SQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLD
                     VTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLS
                     RSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(685..1290)
                     /gene="azoR"
                     /locus_tag="OO244_003814"
     CDS             complement(685..1290)
                     /gene="azoR"
                     /locus_tag="OO244_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5665470.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALHPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1491..5393
                     /gene="hrpA"
                     /locus_tag="OO244_003815"
     CDS             1491..5393
                     /gene="hrpA"
                     /locus_tag="OO244_003815"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5665471.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1375 a         1356 c         1535 g         1281 t
ORIGIN      
        1 gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag tctggttatg
       61 ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg gcgcgataac
      121 aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga cattgccctg
      181 gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc cggtgagcaa
      241 aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac agaatgcggt
      301 tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac tctatttctg
      361 tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg ccgtggcgat
      421 gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat tgggattatc
      481 gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta aaacagttga
      541 tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg aagagcacaa
      601 agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact gaatatctac
      661 agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg ctgcttttgc
      721 gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg cgaagacaaa
      781 tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg tcaccaggtc
      841 cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt ttttacccgt
      901 taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc gtgcaaccag
      961 gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg ccgcaataac
     1021 gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca gagcttcctg
     1081 ctgacgcgga gtcagcggcg catcgctcgg atgcagagcg ccaaccagtt cgccatccag
     1141 taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg agtgcttttc
     1201 gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc ctgccaggat
     1261 gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt tgaatggacg
     1321 atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg cgcaataaat
     1381 cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg gtactctata
     1441 tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt atgacagaac
     1501 aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg ctgcgcgaca
     1561 gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct gatgcacaac
     1621 aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa gtcctgctgc
     1681 gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt cagaaaaaac
     1741 aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg gaaaccggct
     1801 ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt attaaagggc
     1861 tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac cgtattgcgg
     1921 aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc agcgatcacg
     1981 taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg gagatccagc
     2041 aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac gaacgcagcc
     2101 tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt cctgacctaa
     2161 aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac tttaataatg
     2221 cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat cgcccgattg
     2281 ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac gccgtagacg
     2341 aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag cgggaaattc
     2401 gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc ttgccgcttt
     2461 atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc ggacggcgca
     2521 ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt aaatacgtca
     2581 tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg cagcgtttgc
     2641 cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt ggtcgtgtgt
     2701 ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc ccggagttta
     2761 ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg accgcgctgg
     2821 ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc aatatccagg
     2881 atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag gccagcgcct
     2941 ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca cgtctggcgc
     3001 gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt atcacgtccg
     3061 cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca tcggacgaaa
     3121 aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat ctgtggaatt
     3181 atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg tgtcgtaccg
     3241 attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag ttgcgccagg
     3301 tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt gaaattcaca
     3361 tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat aaacaggaat
     3421 ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta ttcaaaaagc
     3481 cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg gggcgcattg
     3541 ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt aaacgcacct
     3601 acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa aaagtcactg
     3661 tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc gattcggcgt
     3721 tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag acgcgtcacg
     3781 cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa cacaaatcac
     3841 gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac cagcgcatca
     3901 gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc agccgcgaaa
     3961 cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg gaaaaaatca
     4021 gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt ttgagctatc
     4081 agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg ccgttactta
     4141 accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc gaattggtga
     4201 ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct gcaccaaact
     4261 acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc gacagccttg
     4321 agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg cactgggatc
     4381 aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac aagaagctaa
     4441 aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg caggaaacgc
     4501 tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg agttttggtc
     4561 agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg tggccggcgc
     4621 tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg gagcaaaaac
     4681 aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg ccgatcaagt
     4741 atttacatga gaagttacca aacaaagcca agctggggct gtactttaac ccgtatggca
     4801 aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg atcgacgcca
     4861 atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa gtgcgcgccg
     4921 aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc actgcggtgt
     4981 tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg gggctttctg
     5041 atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt aacggcttca
     5101 aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg gaaaaactgg
     5161 cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc cagcaggcgt
     5221 ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac gtgaaagaga
     5281 tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt ggtacgcctt
     5341 atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt taaccctgct
     5401 atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt attaattcta
     5461 aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct gcaagatgaa
     5521 taatgtttat ctacagcatt tccttaa
//
LOCUS       DAJTOW010000168         5516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTOW010000168 DAJTOW010000000
VERSION     DAJTOW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.169"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..241
                     /locus_tag="OO244_003816"
     CDS             <1..241
                     /locus_tag="OO244_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665472.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO244_003817"
     CDS             complement(238..1179)
                     /locus_tag="OO244_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5665473.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO244_003818"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO244_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO244_003819"
     CDS             complement(1684..2904)
                     /locus_tag="OO244_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5665474.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO244_003820"
     CDS             complement(2908..3651)
                     /locus_tag="OO244_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5665475.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO244_003821"
     CDS             complement(3542..5008)
                     /locus_tag="OO244_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5665476.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO244_003822"
     CDS             complement(5008..5241)
                     /locus_tag="OO244_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5665477.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..>5516)
                     /locus_tag="OO244_003823"
     CDS             complement(5229..>5516)
                     /locus_tag="OO244_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terL-like domain protein"
                     /protein_id="HCR5665478.1"
                     /translation="EREADIVYDSIGVGASAGAKFSEINADRKSENAYARRVNYQRFN
                     AGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1176 a         1555 c         1388 g         1397 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccagt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctc
//
LOCUS       DAJTOW010000169         5483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTOW010000169 DAJTOW010000000
VERSION     DAJTOW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.170"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            137..1063
                     /gene="tauA"
                     /locus_tag="OO244_003824"
     CDS             137..1063
                     /gene="tauA"
                     /locus_tag="OO244_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5665479.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1076..1843
                     /gene="tauB"
                     /locus_tag="OO244_003825"
     CDS             1076..1843
                     /gene="tauB"
                     /locus_tag="OO244_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5665480.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1840..2667
                     /gene="tauC"
                     /locus_tag="OO244_003826"
     CDS             1840..2667
                     /gene="tauC"
                     /locus_tag="OO244_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5665481.1"
                     /translation="MSVLINEKLHLRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2664..3515
                     /gene="tauD"
                     /locus_tag="OO244_003827"
     CDS             2664..3515
                     /gene="tauD"
                     /locus_tag="OO244_003827"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5665482.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3621..4595)
                     /gene="hemB"
                     /locus_tag="OO244_003828"
     CDS             complement(3621..4595)
                     /gene="hemB"
                     /locus_tag="OO244_003828"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5665483.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5119..>5483
                     /locus_tag="OO244_003829"
     CDS             5119..>5483
                     /locus_tag="OO244_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5665484.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTT"
BASE COUNT         1278 a         1376 c         1499 g         1330 t
ORIGIN      
        1 tgagcatttt taactcgttt tatcttgaat gcaatcggcg cataacttat tgaatcccat
       61 caaatcccgt ctttttttac ccattcccgg ttatcttgaa taaattacct agtcatataa
      121 cacacttctt gccgcactgg cattcatcgc ttttcaggca caggcggtga acgtcaccgt
      181 ggcgtatcaa acctcagccg aaccggcgaa agtggctcag gccgacaaca cctttgctaa
      241 agagagcggg gcgagcgtgg actggcgtaa gtttgacagc ggagccagca tcgtgcgggc
      301 gctggcttct ggcgacgtgc aaatcggcaa cctcggttcc agcccattag cggttgcagc
      361 cagccaacag gtgccgattg aagtcttctt gctggcgtca aaactgggta actccgaagc
      421 gctggtggta aagaaaacta tcagcaaacc cgaagatttg attggtaaac gcattgccgt
      481 accgtttatc tccaccaccc actacagcct gctggcggcg ctgaaacact gggggattaa
      541 accagggcta gtggagattg tgaacctgca gccgcccgcg attatcgccg catggcagcg
      601 gggtgatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg aaaaagacgg
      661 caaggtgctg accgattctg aacaggtcgg gcagtggggt gcgccaacgc tggacgtctg
      721 ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt tcgccaaaag
      781 cgccatcgat gcacagcaac cgtacattgc taaccctgac gcgtggctga aacagccgga
      841 aaacatcagc aaactggcgc gtttaagcgg cgtgcctgaa ggtgacgttc cggggctggt
      901 gaagggaaat acctatctga cgccgcagca acaaacggca gaactgaccg gaccggtgaa
      961 caaagcaatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc cggctgtagc
     1021 gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg cgcagatgct
     1081 gcaaatctct catctctatg ccgattatgg cggcaaaccg gcactggaag atatcaacct
     1141 gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg gtaaaaccac
     1201 cctgctgaat ctgattgccg gttttgtgcc ttatcagcat ggcagcattc aactggcggg
     1261 taagcgtatt gagggaccgg gagcagagcg tggcgtagtt tttcagaatg aagggctgct
     1321 accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag gtatagagaa
     1381 aatgcagcga ctggaaatcg cgcaccagat gctgaaaaaa gtggggctgg aaggcgcaga
     1441 aaaacgctat atctggcagc tttccgatgg tcaacgtcag cgggtgggga ttgcccgcgc
     1501 gctggcggcg aatccccagc tgttattact cgacgaaccg tttggtgcgc tggacgcctt
     1561 cacccgcgac cagatgcaaa ctctgctgct gaaactctgg caggagacgg gcaagcaggt
     1621 gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac tggttctgct
     1681 ttcatccggc cctggccgtg tgctggagcg gctgcggctc aactttgctc gccgctttgt
     1741 tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca tgcgcgaata
     1801 tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct cattaatgaa
     1861 aaactgcatt tgcggcggct gaaatggcgc tggccgctct cgcgtcaggt gaccttaagc
     1921 attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct gcaactgatt
     1981 agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac cattgccgga
     2041 ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct gacgcgcatt
     2101 gtgctggcgc tactcgcggc ggtggtgatc ggtattccgg tcgggatcgc catggggcta
     2161 agcccgacgg tgcgcggcat tctggatccg gtaatcgaac tttatcgtcc ggtgccaccg
     2221 ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc gaagatctta
     2281 ctgatctatt tagcgatttt tgcgccggtg gcgatgtcgg cgctggcagg ggtgaaaagc
     2341 gcgcagcagg ttcgcattcg tgccgcccag tcgctgggtg ccagccgtgc gcaggtgctg
     2401 tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg cattggtctg
     2461 ggtgtgggct ggtctacgct ggtagcggcg gagctgattg ccgcgacacg cggtttagga
     2521 tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtactggc ggggatcgcg
     2581 gtgattgcga ttatcgcctt tcttttagaa ctgggtctgc gcgcgttaca gcgccgtctg
     2641 acgccctggc atggagaagt acaatgagtg aacgtctgag cattaccccg ctggggccgt
     2701 atatcggcgc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat aatcagtttg
     2761 aacagcttta ccatgcggtg ctgcgccatc aggtggtatt tctgcgcgat caagctatta
     2821 cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt caccctgttt
     2881 accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac gataatccgc
     2941 cggataacga caactggcat accgatgtga catttattga aacgccaccc gcaggggcga
     3001 ttctggcagc taaagagtta ccttcgaccg gcggtgatac gctctggacc agcggtattg
     3061 cggcctatga ggcgctctct gttcccttcc gccagctgct gagctggctg cgtgcggagc
     3121 atgatttccg taaatcattc ccggaataca aataccgcaa aaccgaggag gaacatcaac
     3181 gctggcgcga ggcggtcgcg aaaaatcctc cgttgctaca tccggtggtg cgaacgcatc
     3241 cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga attgtcgatg
     3301 tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc actaaaccgg
     3361 agtttcaggt gcgttggcgc tggcaaccga atgatattgc gatttgggat aaccgcgtga
     3421 ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat cgggcgacga
     3481 tccttgggga taaaccgttt tatcgggcgg ggtgatacga gtgcggacgg ttccctcgcc
     3541 cccttgggga gagggttagg gtgagggggc gttcactgta ctttcaacag gttaactccc
     3601 cctttctgag aggaaaaaaa ttaacgcaga atcttcttct cagccaaatc cagcgcaaag
     3661 tagctgaaaa tcagatccgc acccgcacgc ttaattgaac ctaagctttc gagcacgact
     3721 ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcgta ctcaccgctc
     3781 acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac gatgtcgagg
     3841 tacgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc cagcagtgac
     3901 tcacgaatcg cctcgcgacg gttcattggg ttcatctgat agcttttgcg gtcgcctttt
     3961 aatgcgcttc ctgcagcttc acggaacggg ccataaaagg aagaggcgaa cttggtcgaa
     4021 tacgacataa tcgccgtatc tttaaagcct gcagcgtcca gcgcctgacg aatcgcctgt
     4081 acctggccgt ccatcgcggc ggaaggggcg atgaagtctg cgcctgcagc agctgcaacc
     4141 acggcttgct tgcctaaatt ttccagagtc gcgtcgttgt cgacgccatg ctcgcacagc
     4201 acaccgcagt gaccgtgaga agtgtattca cagaaacaag tgtctgacat gacgatcatt
     4261 tctggcacag tctgcttgca gatgcgcgac atacgcgcca ccagtccatc ttcccgccag
     4321 gcgtcgctgc cggtttcatc ggtatggtga gagatgccaa aagtcattac ggaacggata
     4381 ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat gcgcatcacg
     4441 cctggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa gatcggcaac
     4501 accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc aggagatttg
     4561 cgcaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt tgtggaatta
     4621 aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc gcgatgttga
     4681 caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca ataatttcat
     4741 attcactgaa aatattttta atctttattt atagctcttg gttattattt tttggcgttt
     4801 ggttgtgctg ctataaattg aataattcaa actttgttgt atattgataa gatatttatc
     4861 tggtttgtgt ttttgatatt tataacgcgt atgcatcaaa aataacatta cctgcaacat
     4921 actgaaatat cgtcctttta tcaatttgat gcgtgttttt ctccataaat tatatatgtc
     4981 cacattcgga cttaggggaa agaataattg aaccattcgt ctgtaacgca gcataatcgt
     5041 tagcgcgaaa cataatatgt tttctatgca ttgataattg atggatcaac ttattacgtc
     5101 cctgaggagg gatgacaaat gcactcctgg aaaaagaaac ttgtagtatc acaattagca
     5161 ttggcttgca ctctggctat cacctctcag gctaatgcag cgaccaacga tatttctggt
     5221 caaacttaca atactttcca tcactacaac gacgccacct atgctgatga cgtttactat
     5281 gatggttatg taggctggaa caactatgcc gctgatagct attacaacgg cgatatctac
     5341 ccggtcatta ataacgctac cgttaacggc gtgatttcta cctactatct ggacgacggt
     5401 atttctacca ataccaacgc caatagtctg acaatcaaaa acagcactat tcacggtatg
     5461 atttattccg agtgcatgac aac
//
LOCUS       DAJTOW010000170         5414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTOW010000170 DAJTOW010000000
VERSION     DAJTOW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.171"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..511
                     /locus_tag="OO244_003830"
     CDS             <1..511
                     /locus_tag="OO244_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5665485.1"
                     /translation="RGAYNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQ
                     TRLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDM
                     AAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGM
                     DMSHAESAH"
     gene            533..2524
                     /gene="cyoB"
                     /locus_tag="OO244_003831"
                     /pseudo
     CDS             533..2524
                     /gene="cyoB"
                     /locus_tag="OO244_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2514..3128
                     /locus_tag="OO244_003832"
     CDS             2514..3128
                     /locus_tag="OO244_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5665486.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3128..3457
                     /locus_tag="OO244_003833"
     CDS             3128..3457
                     /locus_tag="OO244_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5665487.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3469..4359
                     /gene="cyoE"
                     /locus_tag="OO244_003834"
     CDS             3469..4359
                     /gene="cyoE"
                     /locus_tag="OO244_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5665488.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4543..4854
                     /locus_tag="OO244_003835"
     CDS             4543..4854
                     /locus_tag="OO244_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5665489.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4838..4996
                     /locus_tag="OO244_003836"
     CDS             4838..4996
                     /locus_tag="OO244_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5665490.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4989..5387
                     /locus_tag="OO244_003837"
     CDS             4989..5387
                     /locus_tag="OO244_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5665491.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1180 a         1348 c         1431 g         1455 t
ORIGIN      
        1 tcggggcgct tacaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac
       61 ctccaactcc gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat
      121 ggccggtatg cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat
      181 ctccgccagc tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc
      241 ggatcgcgcc gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga acaccatgtc
      301 tgacatggct gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt
      361 ctccaacgtg aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa
      421 gagcatggac atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat
      481 ggacatgagc cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg
      541 aaaattatca cttgatgcag tcccgttcca tgaacctatc gtcatggtta cgatcgctgg
      601 cattattttg ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta
      661 cctgtggaaa gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat
      721 cgtggcgatt gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca
      781 ggctcttgcc tcggcgggtg aagcgggctt cctgccacct caccactacg atcagatctt
      841 caccgcgcac ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat
      901 gaacctggtg gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa
      961 cttaagcttc tggtttactg ttgttggcgt gattctggtt aacgtttctc tcggcgtggg
     1021 cgaatttgcg cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc
     1081 gggagtcggt gttgattact ggatatggag tctccagcta tccggtatag gtacgacgct
     1141 taccggtatc aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt
     1201 caagatacca gtatttacct aggcatcact gtgcgcgaac gtactgatta ttgcttcctt
     1261 cccaattctg acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt
     1321 ctttaccaac gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg
     1381 ccacccggaa gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc
     1441 aaccttctcg cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat
     1501 caccgtgctg tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa
     1561 cgtaaacgcc ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat
     1621 cttcaactgg ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg
     1681 gactatcggt tttatcgtca ccttctcggt gggcggtatg actggcgtgc tgctggcagt
     1741 accgggcgcg gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt
     1801 gatcatcggc ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc
     1861 gttcggtttc aaactgaacg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg
     1921 cttcttcgtt gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt
     1981 gagccagcag attgacccgc agttccacac catgctgatg attgcagcca gcggtgcagt
     2041 actgattgcg ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg
     2101 cgaccagaac cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac
     2161 ctcttccccg cctccgttct ataactttgc cgtagtgccg cacgttcacg aacgtgatgc
     2221 attctgggaa atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat
     2281 tcatatgccg aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg
     2341 tttcgccatg atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat
     2401 cacctggatc gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat
     2461 cgaaaaactg gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa
     2521 ctgatacttt gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg
     2581 gaaccaaaat cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct
     2641 tgtttgctac ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca
     2701 ttttcgaact gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct
     2761 acggcatggc ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg
     2821 cgttgacctg gttgtttggt gccggattta tcgggatgga aatctatgaa ttccatcacc
     2881 tgattgttaa cggcatgggg ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg
     2941 tcggcacgca cggtctgcac gtcacttctg gtcttatctg gatggcggtg ctgatggtgc
     3001 aaatcgcccg tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt
     3061 tctggcactt cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg
     3121 cgatgtaatg agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta
     3181 catgacaggc tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac
     3241 aggggctgcc tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt
     3301 tctggtgcat ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat
     3361 gacggcgttt gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat
     3421 tatgtggaac ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag
     3481 caatacctgc aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg
     3541 ggattcctgc tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt
     3601 ggggtgtcac tggttgtggc gtcgggttgt gtgtttaaca actacatcga cagggatatc
     3661 gacagaaaga tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct
     3721 gtctcgctgg tgtacgccac cttgctgggt attgctggct ttatgctgct gtggtttggc
     3781 gcgaatccgc tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtctat
     3841 agcctgtaca tgaaacgcca ctcagtctac ggcacattga ttggttcgct ctccggcgct
     3901 gcgccgtcgg tgattggcta ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc
     3961 ctgctggcta tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc
     4021 tttaaggatt accaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg
     4081 gcgaagaatc acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct
     4141 cttggcggtt acgctgggta taaatatctg gtggtcgccg cggcggttag cgtctggtgg
     4201 ttaggtatgg ctctgcgcgg ttataaagtt gctgatgaca gaatctgggc gcgcaagctg
     4261 ttcggcttct ctatcatcgc catcactgcc ctctcggtga tgatgtccgt tgattttatg
     4321 gtaccggact cgcatacgct gctggctgct gtgtggtaac aaaacctctc tattaaaaag
     4381 gtgctacggc acctttttat tgtctgagat cagagatacg gctaataacc gtatctcttg
     4441 acagagtgag cagatgcaca taaattaatc atacggttga tagccgtatt atgtatgaga
     4501 tcgactccct acgatttcat tcagttaatc aaagaagtca taatgcctgg aaaaacagcc
     4561 gctctatacg atgtcgataa aacgctgaaa aatgcgcggg tcgaactcaa aactagtccc
     4621 gacgccaaaa acaaacttcg tgaagctgcg caagccgttg gggtagattt aagcgctttt
     4681 attttgagcg ctgctatgga acgcgccgaa agtgtgcttg ataaccaacg tcgccgtgag
     4741 ctttcgaatc aaagctggga actgatgaac caactcatcg ctgaacctgc tcaaccgacg
     4801 ctcgccctca aggcgttaat gaaaaggaaa aacagcgatg gtcgacaagc atgaagagat
     4861 tactctgccc atagtcctct cctgtaatta tcagtctgat attacttatc ctgggcaaaa
     4921 acagtttgat tgcggtaacc ctgttatcga taaatttgta cgcgctacgc gcatcgctaa
     4981 agaaaagtgt gcgtaatagc gactgtgcgg ctaaagcact tattgacaga caaagtggtg
     5041 aactgattgg catctgtact tttacggcat attcgctgga aagacaacgc gtttctggcg
     5101 tccttcaggg ttcacaacct tcagaaattg gtgttgtcag attagtcatg ttggggggag
     5161 cgcggaagta tcaaaagcgg ggctttggtc aggacctact atgtgatttt tttgaacatg
     5221 taaaaaaaat tcacccggca ttaccaatta aaggggttta tcttgatgct gaccctgccg
     5281 ccattaactt ttatgctcgt ctcggctttg ttcagctttc agcgacacca aatgcttttg
     5341 gtgctgtacc tatgtttttg gcgattcagc atattctcgc ggcttagatt attatcgcca
     5401 cccgccaggg caag
//
LOCUS       DAJTOW010000171         5348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTOW010000171 DAJTOW010000000
VERSION     DAJTOW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.172"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            42..950
                     /locus_tag="OO244_003838"
     CDS             42..950
                     /locus_tag="OO244_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5665492.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(996..2987)
                     /gene="tkt"
                     /locus_tag="OO244_003839"
     CDS             complement(996..2987)
                     /gene="tkt"
                     /locus_tag="OO244_003839"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5665493.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3265..4023
                     /gene="loiP"
                     /locus_tag="OO244_003840"
                     /gene_synonym="yggG"
     CDS             3265..4023
                     /gene="loiP"
                     /locus_tag="OO244_003840"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5665494.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4229..5149)
                     /gene="speB"
                     /locus_tag="OO244_003841"
     CDS             complement(4229..5149)
                     /gene="speB"
                     /locus_tag="OO244_003841"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5665495.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1286 a         1431 c         1433 g         1198 t
ORIGIN      
        1 tcatgtgaca tattcccata ctttactcaa ggaagattgt catgaaagca cctgtttcat
       61 taacctcatt tcgcccacaa aaatctcttg ctatcgccat tggcgtgctt gccgtggttg
      121 tgctgccatt tctctcgtat tacaccgtta atgaaggtga acgcggcatt ttgctgcgct
      181 acggaaaaat cgtaaaagtt gccgaaccgg ggctgggttt taagatccct tttatggaaa
      241 gcgtggagaa aatttccacg cgtaatcagg ctgtggtcta tcagggatta caggcctaca
      301 gccgcgacca gcaaccggca caaatgacag tatcagtcag cttccacatt aaaccttcag
      361 aagcaggcgc ggtttatacc acctacaaca ctattgaagc attaaaagat cgtctaattg
      421 tccgtcagtt gccaactcag ctggagaaca ttttcggcca atataccgcc atcagcgccg
      481 tacaggatcg tacaaaattg gttcaggatc tgcaaaatgc catgcgcaaa gccgttgttg
      541 ggccagtggt aattgacggg gtccagattg aaaatattga tttttctgac gcttatgaaa
      601 agtcgattga aaaccgaatg aaagcagaag tcgccattgc cacgcgtaag caaaatctgg
      661 aaacagagaa aatccaggcg caaatagcgg taactcaggc ccaggcagaa gcagacagta
      721 aactggcggc agccaaagca gaagcggaaa ctattcgcgt aagaggcgcg gcagaggctg
      781 aaactattcg tttaaaaagt gcggctgaag cagaagcaat ccgcctgcgc ggtgaagctc
      841 tgcgggataa tcccggactt gtagccctca ccactgccga acgttgggac ggtaaactgc
      901 ccgataccat gatccccggc agcacagttc cgtttatctc tactaagtaa aaaaagggcc
      961 gctttcgcgg tccttctcac aaaacgtctt actgattaca gcagcgcttt tgctttcgca
     1021 acaacgttat caacagtgaa gccgaactct tcaaacagca gctctgccgg agcagattca
     1081 ccgaaggtgg tcatgccgac gatagcgccg ttcaggccaa catacttgta ccagtagtca
     1141 gcaatacccg cttctaccgc aacgcgtgca gtaaccgctt tcggcagtac ggattcacgg
     1201 taagccgcat cctgcttgtc aaatgcgtcg gtagacggca tggacaccac gcgcgctttc
     1261 acgccttcgg cagtcagttt ttcgtaggca gcaaccgcca gttcaacttc agaaccggtg
     1321 gcgatgaaga tcagttccgg ctgaccagca cagtctttca gcacataacc accgcgcgcg
     1381 atgtttgcca gctgctcttc agtacgttcc tgctgcgcca ggttctgacg ggagaggata
     1441 agcgcagtcg ggccgtcctg acgctcaacg ccgtatttcc acgcgaccgc ggattcaacc
     1501 tggtcacacg gacgccatgt agacatgttc ggggtcacgc gcagagaagc gacctgctct
     1561 accggctggt gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt gtaaaccatc
     1621 acctggcgct gtttcatcag cgcagccata cgtacggcgt tacgtgcgta ttccacgaac
     1681 atcaggaagg tggaggtgta cggcaggaag ccaccgtgca gggagatacc gttagcaatc
     1741 gcggtcatac cgaactcgcg aacaccgtag tggatgtagt tacctgcagc atcttcgttg
     1801 attgctttag aaccagacca cagggtcagg ttagacggtg ccaggtcagc agagccgccg
     1861 aggaattcag gcaacagcgg gccgaacgct tcgatagcat tctgcgacgc tttacggctg
     1921 gcgattttcg ccggattagc ctgcagttta gcgataaact ctttcgcttt ggcgtcgaag
     1981 tcagacggca tttcgccttt catacggcgg gtaaattcag ccgcttcctg cggataagct
     2041 ttcgcgtaag ccgcaaactt ctcattccat gcagattctt tcgcctggcc tgcttctttc
     2101 gcatcccact gagcatagat ttcagacggg atttcgaacg gtgcatattt ccagcccagc
     2161 tgttcgcggg tcagggcaat ttcagcgtcg cccagcggcg caccgtggga gtcgtgagta
     2221 ccggctttgt tcggggaacc gaaaccgatg atggttttgc acatcagcag ggacggtttg
     2281 tcagtcactg cgcgcgcttc ttcaactgcg cgtttgatgg atgccgcgtc atgaccgtcg
     2341 atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca ttgctgtgtc gtcggtgaac
     2401 cagccttcaa cgtgaccatc gatagaaata ccgttgtcat cgtagaacgc aatcagttta
     2461 cccagcttca gcgtacccgc cagagagcaa acttcgtggg agatgccttc catcatgcag
     2521 ccgtcgccca tgaaggcgta ggtgtagtgg tcgacaatgt cgtggcccgg acggttaaac
     2581 tgcgccgcca gcgttttttc tgcaatcgcc ataccgactg cgttggcaat accctgaccc
     2641 agcggaccgg tggtggtttc cacaccagcg gtataaccta cttccgggtg gcccggagtt
     2701 ttagagtgca gctgacggaa gtttttcagt tcttccatcg gcagatcgta accggtgagg
     2761 tgcagcaggc tgtagatcag catggagccg tggccgttgg acagcacgaa gcggtcacgg
     2821 tcagcccagg acggattctg cgggttgtgt ttcaggaaat cacgccacag gacttcggca
     2881 atgtcagcca tacccatagg ggcacccggg tgaccggatt tggctttctg tactgcgtcc
     2941 atgctcagcg cacgaatagc attggcaagc tctttacgtg aggacatttt gactccagat
     3001 cggatgatga agggcacgcc cttaacgact tgacgacagc gcgttttggg ctacgccgga
     3061 aaatttgcca acaatttacc gcaagccgcg cgtcatgtac atggaacatc cttttgccgc
     3121 ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct gctcgcccgt ccgctgcgct
     3181 tttccttata ctgagactga gcgtcgattc acctgcaaac ggcgcatttt tagaataatc
     3241 ctgaccttgt gcggaagaga aaacatgaaa attcgcgcct tattggtagc aatgagcgtg
     3301 gcaacggtac tgaccggttg ccagaatatg gactccaacg gactgctctc atcaggagcg
     3361 gaagcttttc aggcttacag tttgagtgat gcgcaggtga aaaccctgag cgatcaggca
     3421 tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca atagcgaata cgctaaacgt
     3481 ctgacaacta ttgccaatgc gctaggcaac aatatcaacg gtcagccggt gaattacaaa
     3541 gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta acggctgtat ccgcgtctat
     3601 agcgggctga tggatatgat gacagataac gaagtcgaag cggtgatcgg tcacgagatg
     3661 gggcacgtgg cgttaggcca tgtgaagaaa gggatgcagg tggcgcttgg tacaaatgcc
     3721 gtgcgagtag ctgcggcctc tgcgggcggg attgtcggca gtttatctca atcacaactt
     3781 ggtgatctgg gcgagaaatt agtcaattcg caattctccc agcgccagga agcagaagcc
     3841 gatgattatt cttacgatct tctgcaccaa cgcggcatca gcccggcagg tcttgccacc
     3901 agctttgaaa aactggcgaa gctggaagaa ggccgtcaaa gctcaatgtt tgacgaccat
     3961 cctgcatccg ccgaacgtgc ccagcatatt cgcgatcgca tcagcgcgga tgggattaag
     4021 taaagcctag tggtgtcgaa cgtaggtcag ataaggcgtt cacgccgcat ctgacattaa
     4081 tggcacattt gttaccttgt gcgcattgcc ggatgcgatg ctggcgcatc ttatccggcc
     4141 tacgggtgcc gaacgtaggt cggataaggc gttcacgccg catccgacat taatggcacg
     4201 ttttacccgt gcgcatcgca tctggtgctt actcgccctt tttcgccgcc tgaatataca
     4261 gcatctccag cgccagcgtc gccgcagcca gagcggtgat ttccgactga tcgtatgccg
     4321 gagccacttc cactacgtcc atcccaacga tgttgagatc tttcaggccg cgtaccagtt
     4381 taatggcgcg atcggaggtc aggccgccaa tcactggcgt accggttccc ggcgcgaaag
     4441 caggatccag gcagtcgata tcaaaagtca ggtaaaccgg catatcaccc acaatctgtt
     4501 tcacctgggc gataacgtca tccacgctgc gatcgttcac ctggcaggcg tccagcacgg
     4561 taaagccgtt gtctttatca aactcggtac gaataccaat ctgcacggaa tgattcgggt
     4621 cgatcagacc ttctttcggc gcggtataga acatagtgcc gtggtcaaat tcacaaccgt
     4681 tcgcataggt atcggtgtgg gcgtcaaagt gtaccagcgc cattttgccg aaatgcttcg
     4741 catgagcacg cagcagcggc agcgtaacaa agtggtcacc accgaaggag agcatacgct
     4801 taccggcagc cagcagcttc tcggcgtgcg cctgcagctt ttcgctcatc tcacgggcat
     4861 cgccaaaggc atataccaga tcgccgcagt ccacgacgtt cagacgctca cgcatgtcga
     4921 aattccacgg gaagcggttg tgttcccagg ccagattcgt cgaaacctga cggatcgctg
     4981 ccggaccgtg gcgaccaccc gcacgaccag aagtggccat atcgaacggc acgccagtaa
     5041 tcacccagtc tgcatcgctg tcatacggct ggaagttcat cggcaggcgt aaaaaaccaa
     5101 aggcattgga aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg gatggactcc
     5161 tattacaaag tcgatataaa aaaacccttc cgcgtcgtta ggcccgacga ggaagggttg
     5221 gatttgcgaa acacaagttt gtgctaatta tcgccttaaa tgggcgaggc atcaagcgag
     5281 gttgctattt ccgacacggt ggcggcgcat agccattgtt atctgccgga aaggcttgtc
     5341 cagagcac
//
LOCUS       DAJTOW010000172         5340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTOW010000172 DAJTOW010000000
VERSION     DAJTOW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.173"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO244_003842"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO244_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5665496.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO244_003843"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO244_003843"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5665497.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO244_003844"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO244_003844"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5665498.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO244_003845"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO244_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5665499.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAJTOW010000173         5241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTOW010000173 DAJTOW010000000
VERSION     DAJTOW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.174"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..458)
                     /locus_tag="OO244_003846"
     CDS             complement(<1..458)
                     /locus_tag="OO244_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665500.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKL"
     gene            complement(526..2475)
                     /locus_tag="OO244_003847"
     CDS             complement(526..2475)
                     /locus_tag="OO244_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5665501.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKCYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2560..2895)
                     /locus_tag="OO244_003848"
     CDS             complement(2560..2895)
                     /locus_tag="OO244_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5665502.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2895..3251)
                     /locus_tag="OO244_003849"
     CDS             complement(2895..3251)
                     /locus_tag="OO244_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5665503.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3251..4747)
                     /locus_tag="OO244_003850"
     CDS             complement(3251..4747)
                     /locus_tag="OO244_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5665504.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4744..4905)
                     /locus_tag="OO244_003851"
     CDS             complement(4744..4905)
                     /locus_tag="OO244_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5665505.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4915..>5241)
                     /locus_tag="OO244_003852"
     CDS             complement(4915..>5241)
                     /locus_tag="OO244_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665506.1"
                     /translation="KGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLLDLN
                     RYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEHHLE
                     MRFPQN"
BASE COUNT         1237 a         1539 c         1134 g         1331 t
ORIGIN      
        1 ttcaatttgc aattttcgta ggtcactccc gactcgccat taacattaac tccagctctg
       61 atattcccgt tttcttttac atctacaaaa aactggcctc ttggcatctt gaacgcaaat
      121 gtaaaaccaa cagtatctga atcaaaatca tcatctttat atattgggat caaaaatgtc
      181 atcgtcgctg gtgatgcatt gtttgcaaaa tcaaatacac ctatactttt gccgaattca
      241 taagaagctt ttctcaatat aatcgcaccg ctggcagaag gggatgggca cgatatcctc
      301 agttgtctga atccatctgt atccaagtca acgtcattat ctgcagatgt tttaacgtta
      361 ctaataaatc gtgaaacaat ctcctcattt gttagtattt tcgcctgagc acagcaagag
      421 gttaatattg caaccgtcat taaaaatggc tttatcacaa aacaaactcc attgttagca
      481 tgttacgcga taattttgcc tcatctttca ccacaaaaaa agaactcacc ttttaaatgg
      541 agaataccca acatcatggg tgattttcat aaagggatcg cctgttttcg gcaaatcgat
      601 aacgcgcatt ccaggcggcg cgttgtcaaa cgtcacctta agttcgctgc gggttgccgg
      661 tgaagatgca aggcttaaca gctgattcct ttccagtgat acattcgggg tgtagcattt
      721 atcgggagat aaatacccat caccacgtgg catctgccat ccggtcatgt catagacaaa
      781 atcatggaac tgtttgcccc actcgtcgaa cttatcattc agaccaaatc cactgttcag
      841 tgcatcagcc acaaaatttt taacaagcca gggatgttct ttttcaaact cctgcgccat
      901 catgcccagt tcaagcaatc caccaatcag actgatcttt ccgaggccct ttaatttcag
      961 ccctgttttt cctgccgccg ttgtccattg attctgggcg actgttgccg cacgaatact
     1021 tgccaccgcc ttcacaccaa tatacagggc agaaaccgtt gctattgttt ttactgcctg
     1081 ttcccatccc cccattgctc tgacaacctg atcaacctcc tgccagactt ttttcacaac
     1141 cggaccgacc gtttcccagt tatcaattat gagatatgca ccaccgacga gaagcgcaat
     1201 taatcccttt gccggcgtca tattcatcac gctacccatg atttttgtta tgcgtgtcaa
     1261 tgtgccaatc gccacgccca tcgtcagtaa tgccgcacca gttttcgcga tggtttttac
     1321 gacctcagga ttttctctga caaacgtgcg cacttcttcc agaaaaggct tcatctcttt
     1381 tatgccttca ttgagtgaag gcaggaaggt ttcccccagc gtggaagaaa tcgcattgat
     1441 ctggttctgc agcagcaaca actgattttc tgtcgtcgcg gcacgggcgg catattcttt
     1501 ctgcattgag ccgccatact gctgcgcatc cgccacacga ttaaagttgg ttcgcaacaa
     1561 atccagattc gtgagcagcg gcgcaatggc ccccagggat tccttcccga acagcgcttt
     1621 cagcacagca gcctgttttt ctttcggcac ttttgccaga gaatccagta cgtgcagcat
     1681 ggccccgcgg gcgtctttct gcatatccgc cgccagtttt ttcgggctaa tgcgcaacga
     1741 gcggagcact tttttctgcg atttggtggc agaatccctc gctgtcagcg acaacataaa
     1801 attctttatc cccgtcgcag caatttctga ctccaccccc attccggcaa tggttgcccc
     1861 cattgcggca atctctccgg aggccacacc cgcaacactg cctaaagggc caatacgggt
     1921 cacaacatca gaaatctttt tcgcacttgc aggaccggta ttgccaagat agttaatctt
     1981 atccgcaagc cctgccacct ctccctgtgt cagtttaaag gcagtgcgcc actgcgccat
     2041 catctgaccg gattcttctg ccgtggtgtc aaaggccacg cccatcttca cggcatcgtc
     2101 agtaaactgc atcagctcgt cacgggcgat accagcctga ccacccgccg ccacaatttc
     2161 ggcgatacct tccgccgaca tgggcagttc tgttgacagg tcgcgcacct gctccgtcat
     2221 tgccttaaac gcttccggcg tatccagacc atccaccact ttccggacat ccgccatttt
     2281 tgattcaagg gcaatggcag atttgaccgg gagcgccagc gcccccagta ctgcggttcc
     2341 ggcacccgct gcgcccagtg acaggctggc aaactccttt ttaaaacctt tcagctggcg
     2401 ctgcattccc ttcagcggtg ccgatacctt atccacggca gtgatgatcg ccttcagctg
     2461 aaaactgtca gccatgcttc atctcctcgt taatgcggac ggcctcggct tccagttctg
     2521 taaactgcga aacagccacc cgtcgcagct ccagtggatt cagtttccag aaccaggcaa
     2581 cattgtagag tcgtttccgg aggtgcttcc cgtctccgac tgggtaaaaa aacgcaggat
     2641 ctgcatgctg gtcttaaaaa tatccagttt tgccatctgc gccgccgatg aacacgggat
     2701 ccccgccagc aacgggatat atttcagtgc cacctggctg tccagtttaa tactgccttc
     2761 accggaaata atgaaaggaa aacccagcgc ctcgatttcg tcatacgtgg gctcgcgcaa
     2821 ctccagcaca tgcagcattt cgttatgcgc tgtcaccggt ttttttaaaa ctatttctgt
     2881 aacgttcatt gccattactg ataccctccc tcttcgccgt ggaattcaag atctgccgtg
     2941 ccttcttcgg cattatgatt agcctctccg tgcagccatg ccgccgaaag cacatacacc
     3001 atgccgtttg ccagctcagc ggtaatcgtc atctggtctg aggtggtaat tttgtcgacc
     3061 ggaaagtttt tgggcacttt aaacgtgccc ttaacgtaag gtgaccgcca ggtctccttg
     3121 taatccacat cccctgccat gccgacgatg tcatcccgga cattggtgtt catcggcact
     3181 tcaatgccac ctgtcagcga taactgctga ccatccactt taaaaaaaca ggttccggcg
     3241 atctttgcca ttatgctgac tcctctgaat actgaagacg gaactggtta accaccgcaa
     3301 agacacgtaa ctgattaaca taatccggcg ggaacagcgt attcagtcgg ttcgggttat
     3361 ctgcatcacg ctcaactatc agatactgtt taaacagatc gtaattttcc acaataccgg
     3421 cacgctccat ctgacgatat gtcgccagaa gttccccttt gataacggca ggagtgacaa
     3481 tcgcctgccc cggaccaaaa cgggtaccat catttgccag cttgtgacgt ccgtacttgc
     3541 ttgtgatgac cgatttcagt ttgcgaagaa catatgcact ggtatgcaga gtttcactgt
     3601 ccagatagct gttgtctgcc acgccatacg cattcttttt gtaggtggtt acggaacgct
     3661 ggatctgcaa cgtgccgcct tccacataag ccgtcgccac gccgtgagat aaaagggtct
     3721 gctgctctgt catgatgaat cgcttacctt tcggtgccgg aagcatcccc accagctccc
     3781 ccgtctgtgt cggacgggca ggatcattcc ggataaatac cgcttcacgg gcaaggcgac
     3841 tggcaaccag ttcatcgaca ggcgactggg tttctttttc gtaaccggca agcgtgatat
     3901 gttgctgatt atgcatatct ccggcaccaa ccagctctga cagcgttccc agttttgcgg
     3961 tatagacatg cccgtataac tggcgcgcat aactccagcg accgctgctg tcattcattt
     4021 cggtcatcat catattgatg gaggcggcat cgttgaacgg cagaccgata aagtcgaatg
     4081 cctcatcgcc catagcagca acagcggcgg taagatcagg cgctccgcta ccggcagcac
     4141 cgacttccgt caccacccga agccctgctg gcaaaatctc accaccacca gaaccataat
     4201 aattcagaca gaccggtaac tcattaccat acagcccctt atgacgggca gtcagcgtca
     4261 ccacgcctgc atcagatgac gccgtaaacg gcagggtgat aaccccgttt accgcttcct
     4321 taatcgcggt ggcaaccgca gtggcatcat cgccattcac cacaggcacc tgtacacggg
     4381 agcgcccgac atacagactc agggtgccgc tttcctctgc ttctccggca accgtcaccc
     4441 tgaccgtcgc cgccgcccct ctggcttccg gtactgcaat aacatacagt tcaccgaaag
     4501 gatctgtctg acggtagact tccaccatac gcgccagctg gctccccgcc ccacaaatct
     4561 ggcgggcata atctgccgat ggcatcagca ccaggctgtt aacctcaatg gctgcatcgt
     4621 tgctggcatg cccgatcagt aatgcaggcg cgctggtcac cgctgtattt gccgcagaat
     4681 tgttcatctc ggcgtagaac aacggcacca gcgtattcga cggaacagta ttaaaactta
     4741 tcgtcatgat tttttagcct tatcctgttt aacacgtacc acatcacccg ccgctatacg
     4801 gcggaaccag tagctgcttt cttccacatt tcgcccttcg gaaggcaaaa ggtctccacg
     4861 ggcagggtca ggaaccgacc gccctttcag gggtttcaca aacatgaggg actcttaatt
     4921 ctgagggaaa cgcatttcca gatggtgctc aatgtcacca tccgggccag taccaggatc
     4981 gatgtaatcc acatcaatac tcagcaggga taaatccggc agggcattca cgtcctctgc
     5041 ctgtcgcgtg tcttcttccg tgatttcata cttcgccgta aaatcaaact ggtaatacag
     5101 ttcgtaacgg ttcagatcca gcagggtgcc accgacataa acaatctcgc ccccttgcgg
     5161 gtccggcatc catccaagca gggctttcca gagttctctc cggacatcat gaacggcgtc
     5221 ataggctgcc cactgccctt t
//
LOCUS       DAJTOW010000174         5178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTOW010000174 DAJTOW010000000
VERSION     DAJTOW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.175"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(8..202)
                     /locus_tag="OO244_003853"
     CDS             complement(8..202)
                     /locus_tag="OO244_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665507.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKVMLPTY"
     gene            278..2839
                     /gene="mutS"
                     /locus_tag="OO244_003854"
     CDS             278..2839
                     /gene="mutS"
                     /locus_tag="OO244_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5665508.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2945..3597
                     /gene="pphB"
                     /locus_tag="OO244_003855"
                     /pseudo
     CDS             2945..3597
                     /gene="pphB"
                     /locus_tag="OO244_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3648..4415)
                     /locus_tag="OO244_003856"
     CDS             complement(3648..4415)
                     /locus_tag="OO244_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5665509.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4611..5178
                     /locus_tag="OO244_003857"
                     /pseudo
     CDS             4611..5178
                     /locus_tag="OO244_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1289 a         1333 c         1332 g         1224 t
ORIGIN      
        1 cactaaatca gtaagttggc agcattacct taatcttaaa gggcactatg actctaaaga
       61 agaagggttc gccaatcgat acaattttgc gtacttgctt cataagcatc acgcaaaagc
      121 tgcaaaacag catctttccc ggaaccagca tcaagaactc gccgttcgct tcttcccctg
      181 aaatgattaa ctccggtatc atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc
      241 accccccatt taatatcagg gaaccggaca taaccccatg agtgcaatag aaaatttcga
      301 cgcccatacg cccatgatgc agcagtatct caagctgaaa gcccagcatc ccgagatcct
      361 gctgttttac cggatgggtg atttttatga actgttttat gacgacgcaa aacgcgcgtc
      421 gcaactgctg gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat
      481 ggcggggatt ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga
      541 gtccgttgcc atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg
      601 caaagttgtg cgtatcgtta cgccaggcac catcagcgat gaagccctgt tacaggagcg
      661 tcaggacaac ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct
      721 ggatatcagc tccgggcgtt ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc
      781 agagctgcaa cgctctaatc cggcggaact gctgtatgca gaagatttcg ccgagatgtc
      841 gctgattgaa ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac
      901 cgcgcgccag cagttgaatc tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga
      961 gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca
     1021 acgcacgacc ctgccgcata ttcgttctat caccatggaa cgtgagcagg acagcatcat
     1081 tatggatgcc gcgacacgtc gtaacctgga aattactcag aacctggcgg gcggtgcgga
     1141 aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa
     1201 acgctggctg catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat
     1261 tggcgcattg caggatttca ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct
     1321 ggaacgtatt ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat
     1381 gcgccacgct ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc
     1441 accggtacag gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg atctgctgga
     1501 gcgagcaatc atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg
     1561 ctataacgaa gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga
     1621 gcgtctggaa gtccgcgagc gtgaacgtac cggcctggac acgctaaaag ttggctttaa
     1681 tgcggtgcac ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa
     1741 ctacatgcgt cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga
     1801 gtacgaagat aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta
     1861 tgaagagctg ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc
     1921 gctggcggaa ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac
     1981 ctgcccgacc ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt
     2041 tgaacaagta ctgaatgagc catttatcgc taacccgctg aatctgtcgc cgcagcgccg
     2101 tatgttgatt attaccggcc cgaacatggg cggtaaaagt acctatatgc gccagaccgc
     2161 actgattgcg ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg
     2221 acctatcgat cgcatcttta cccgcgtagg cgcggcagat gacctggctt ccgggcgctc
     2281 aacctttatg gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag
     2341 tctggtgtta atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc
     2401 gtgggcgtgc gcagaaaatc tggcgaataa gattaaggca ttgacgctgt ttgccaccca
     2461 ctatttcgag ctgacccagc taccggagaa aatggaaggc gtcgccaacg tacatctcga
     2521 tgcgctggag cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag
     2581 caaaagctac ggtctggcgg ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg
     2641 cgcacggcaa aaactgcgtg agctggaaag catttcgccg aacgccgccg cgacgcaagt
     2701 ggatggtacg cagatgtctt tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc
     2761 actggaaaat ctcgacccgg attcactgac tccgcgtcag gcgctggagt ggatttatcg
     2821 cttgaagagt ctggtgtaat aacaattccc gatagtcttt tgctatcggg aatattaacg
     2881 acaactgacg aatcaaataa aaataccctg tataatagga aagcttattt tacagggtaa
     2941 aaccatgcca tctacacgct accaaaaaat caatgcccat cactatcgcc atatatgggt
     3001 cgttggtgat attcatggtg aatatcagtt attacaatcc cgcttacatc aactctcttt
     3061 tttccccgaa accgacttac ttatttctgt cggcgataat attgatcgtg ggccggagag
     3121 tcttgacgtc ctgcgcctgc taaaccagcc ctggtttatc tccgttaaag gcaaccacga
     3181 agcaatggcg ctggatgcgt tcgcgaccgg cgatggcaat atgtggcttg ccagcggtgg
     3241 tgactggttt ttcgatttaa atgattgcaa caagaggcaa tagatctgtt gctgaaattc
     3301 catcaccttc cacatattat tgaaatcact aacgacataa taaaatatgt catcgcacat
     3361 gcagattatc cgggtgatga atatctcttt ggcaaagaaa tagcggagag cgaattactc
     3421 tggcctgttt atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat aaacggcgct
     3481 gattatttta tatttggaca tatgatgttt gataacattc agacgttcgc taaccagatt
     3541 tatattgata ccggatcgcc gaaaagtggg cggctgtcat tttataaaat aaggtagtct
     3601 catgcttctt ctgtgaagca tgagtaacca gatgttattg caggccatta ttcatttttc
     3661 gctaccagca aagagagatc ctgcttcgcc agcgcgcgac tggcactctc cggcaaaccg
     3721 tcgtctgtaa taatctgatc aaactcgctt aatggtaacg ccagccatgt cgccacctga
     3781 ccatatttcg tcgcatcaca gaccaaaact cgctggcggc tggcactggc aatcgcccgt
     3841 ttcaccgtga ctttatcttc cgctggcgta gaaatccccc gcacactcca tgacgatgca
     3901 gaaataaaag cctgatcaat catcaggctg cacagcatgg tcgcagcggc ttccccgaca
     3961 caggaacggt tttcccgaca cactgcaccg ccagtgtgaa taattgtgca attactgttg
     4021 tcgagcaagt agttcgcaat aacgaaatcg tttgtgacca cagtcagtga ctccatgtga
     4081 atcagatgct gtgctatcgc taacgtggtc gttcccgcat ccagatagat acaacttccc
     4141 ggcagaacaa gacttgccgc cagcttgcca atagccgctt tttgcgtcat tgccagcgca
     4201 gtttttacct gatgagaagg ttcatgcgcc acgcgtcccg gagactggac gcctccggac
     4261 accagcacaa cggctccctg ctgctccagt ttttgtaaat cccgacgaat ggtcatatgt
     4321 gacacattaa ttctgtccgt tagttcagca atactgacaa tgcctttttc agctaccatc
     4381 tcaaggatga tttggcgacg ctctacgggt atcaactttt gctccttcct ttgtcctgct
     4441 gacattctac gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt agttcacata
     4501 aaataacaca aaatgttaat ttatgtgaat tatctcacca tatcgttatc tcccagcgct
     4561 tatgttcaca atatcaaaca aaatatcact taaattaaca aggagagcag atgaaaacgg
     4621 gatctgagtt tcatgtcggt atcgttggct tagggtcaat gggaatggga gcagcactgt
     4681 catgtgtccg cgcaggtttt tctacctggg gcgcagacct gaacagcaat gcctgcgcta
     4741 cgttgaaaga ggcaggtgct tgcggggttt ctgataacgc cgcgacgttt gccgaaaaac
     4801 tggacgctct gctggtgctg gtggtcaatg cggcctaggt taaacaggtg ctgtttggtg
     4861 aaacaggcgt tgcacaacat ctgaaacccg gtacggcagt aatggtttct tccactatcg
     4921 ctagtgctga tgcgcaagaa attgctaccg ctctggctgg attcgatctg gaaatgctgg
     4981 atgcgccagt ttctggtggt gcagtaaaag ccgctaacgg tgaaatgact gtcatggcct
     5041 ccggtagcga tattgccttt gaacgactgg tacccgtgct ggaagccgtt gccggaaaag
     5101 tttatcgcat aggtgcagaa ccgggactag gttcgaccgt aaaaattatt caccagttgt
     5161 tagcgggcgt acatattg
//
LOCUS       DAJTOW010000175         5158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTOW010000175 DAJTOW010000000
VERSION     DAJTOW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.176"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            190..447
                     /locus_tag="OO244_003858"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO244_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO244_003859"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO244_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5665510.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO244_003860"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO244_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5665511.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO244_003861"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO244_003861"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5665512.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO244_003862"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO244_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO244_003863"
     CDS             complement(3605..4420)
                     /locus_tag="OO244_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5665513.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5158
                     /locus_tag="OO244_003864"
     CDS             4517..>5158
                     /locus_tag="OO244_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5665514.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1375 a         1177 c         1227 g         1379 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc ttgttcac
//
LOCUS       DAJTOW010000176         5153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTOW010000176 DAJTOW010000000
VERSION     DAJTOW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.177"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            2..211
                     /locus_tag="OO244_003865"
     CDS             2..211
                     /locus_tag="OO244_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5665515.1"
                     /translation="MFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(383..712)
                     /locus_tag="OO244_003866"
     CDS             complement(383..712)
                     /locus_tag="OO244_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5665516.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(717..902)
                     /locus_tag="OO244_003867"
     CDS             complement(717..902)
                     /locus_tag="OO244_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5665517.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(899..3538)
                     /locus_tag="OO244_003868"
     CDS             complement(899..3538)
                     /locus_tag="OO244_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5665518.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3746..4735
                     /gene="ldhA"
                     /locus_tag="OO244_003869"
     CDS             3746..4735
                     /gene="ldhA"
                     /locus_tag="OO244_003869"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5665519.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4846..>5153
                     /gene="hslJ"
                     /locus_tag="OO244_003870"
     CDS             4846..>5153
                     /gene="hslJ"
                     /locus_tag="OO244_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5665520.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1306 a         1320 c         1201 g         1326 t
ORIGIN      
        1 aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt
       61 ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca
      121 ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct ttgggatgac
      181 gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta ctgcaacgcc
      241 aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga
      301 gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta
      361 tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta
      421 catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat
      481 ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa
      541 tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa
      601 atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc
      661 tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag
      721 aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc
      781 tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca
      841 atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc
      901 attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct
      961 gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat
     1021 tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac
     1081 ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg
     1141 accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt
     1201 tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattagcccg ctatttgcca
     1261 gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa
     1321 atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga
     1381 gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca
     1441 cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc
     1501 acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt
     1561 cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac
     1621 ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc
     1681 ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa
     1741 cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct
     1801 ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc
     1861 agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct ttaaagagga
     1921 aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc
     1981 cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct
     2041 gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca
     2101 tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt
     2161 catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt
     2221 ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat
     2281 ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac
     2341 gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga
     2401 tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca
     2461 gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt
     2521 ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc atgcttaatt
     2581 ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacat
     2641 tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg actttgacac
     2701 ccagtcggcc actcaaagga aaaccagcat acagccagct ccagcgacca tcgcttacgg
     2761 tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg
     2821 ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg
     2881 gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca
     2941 cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga
     3001 catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt
     3061 cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc
     3121 attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg
     3181 actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg
     3241 ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg
     3301 aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg
     3361 gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga
     3421 gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca
     3481 acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg
     3541 gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt
     3601 taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata
     3661 tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag cttaaatgtg
     3721 attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga
     3781 caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt
     3841 tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt
     3901 aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg ttaaatatat
     3961 cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct
     4021 gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat
     4081 gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt
     4141 ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac
     4201 cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc
     4261 gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc
     4321 aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta
     4381 ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac
     4441 cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat
     4501 tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc
     4561 caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt
     4621 taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct
     4681 gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg
     4741 cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct
     4801 attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc
     4861 gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt
     4921 acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg
     4981 accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc
     5041 atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg
     5101 ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga taa
//
LOCUS       DAJTOW010000177         5091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTOW010000177 DAJTOW010000000
VERSION     DAJTOW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.178"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..736
                     /locus_tag="OO244_003871"
     CDS             <1..736
                     /locus_tag="OO244_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5665521.1"
                     /translation="SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVT
                     YPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGS
                     GNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFY
                     LSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLS
                     ATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1070..3349
                     /gene="ydeP"
                     /locus_tag="OO244_003872"
     CDS             1070..3349
                     /gene="ydeP"
                     /locus_tag="OO244_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5665522.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3597..3800
                     /gene="safA"
                     /locus_tag="OO244_003873"
     CDS             3597..3800
                     /gene="safA"
                     /locus_tag="OO244_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5665523.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3868..4629
                     /gene="ydeO"
                     /locus_tag="OO244_003874"
     CDS             3868..4629
                     /gene="ydeO"
                     /locus_tag="OO244_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5665524.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1456 a         1084 c         1135 g         1416 t
ORIGIN      
        1 gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa
       61 cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat cgctgtactg
      121 gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga
      181 taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg cggccggtgg
      241 agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa ttgcaacatt
      301 aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat
      361 gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc
      421 tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta
      481 tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc
      541 aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg
      601 agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac
      661 ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac
      721 ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg
      781 cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat
      841 tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac tatgagagat
      901 gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga
      961 gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
     1021 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
     1081 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
     1141 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
     1201 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga
     1261 catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc
     1321 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
     1381 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
     1441 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
     1501 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
     1561 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
     1621 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
     1681 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
     1741 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
     1801 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
     1861 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
     1921 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1981 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     2041 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     2101 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     2161 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     2221 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     2281 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     2341 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     2401 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     2461 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     2521 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     2581 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     2641 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     2701 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     2761 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     2821 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     2881 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     2941 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     3001 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     3061 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     3121 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     3181 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     3241 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     3301 tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     3361 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     3421 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     3481 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     3541 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc
     3601 atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg
     3661 taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc
     3721 agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     3781 gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat
     3841 aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat
     3901 catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg
     3961 atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc
     4021 ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
     4081 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
     4141 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
     4201 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
     4261 gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
     4321 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta
     4381 aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa
     4441 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
     4501 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
     4561 aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta
     4621 gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg
     4681 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
     4741 catccatcac atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt
     4801 cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg
     4861 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc
     4921 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     4981 ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg
     5041 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct g
//
LOCUS       DAJTOW010000178         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTOW010000178 DAJTOW010000000
VERSION     DAJTOW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.179"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="OO244_003875"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="OO244_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5665525.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="OO244_003876"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="OO244_003876"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5665526.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="OO244_003877"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="OO244_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5665527.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="OO244_003878"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="OO244_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5665528.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO244_003879"
     CDS             4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO244_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5665529.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1209 c         1255 g         1416 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
     4921 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
     4981 acgttatccg catatcagca tttgctggga acagagcgac acct
//
LOCUS       DAJTOW010000179         5017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTOW010000179 DAJTOW010000000
VERSION     DAJTOW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.180"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(681..1097)
                     /locus_tag="OO244_003880"
     CDS             complement(681..1097)
                     /locus_tag="OO244_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665530.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1124..1264
                     /locus_tag="OO244_003881"
                     /pseudo
     CDS             1124..1264
                     /locus_tag="OO244_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1264..2289
                     /locus_tag="OO244_003882"
     CDS             1264..2289
                     /locus_tag="OO244_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5665531.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2342..2431)
                     /locus_tag="OO244_003883"
     tRNA            complement(2342..2431)
                     /locus_tag="OO244_003883"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2395..2397),aa:Ser,seq:cga)
     gene            2525..3322
                     /gene="mtfA"
                     /locus_tag="OO244_003884"
     CDS             2525..3322
                     /gene="mtfA"
                     /locus_tag="OO244_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5665532.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3423..3498
                     /locus_tag="OO244_003885"
     tRNA            3423..3498
                     /locus_tag="OO244_003885"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3456..3458,aa:Asn,seq:gtt)
     gene            3660..4922
                     /locus_tag="OO244_003886"
                     /pseudo
     CDS             3660..4922
                     /locus_tag="OO244_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1386 a         1183 c         1122 g         1326 t
ORIGIN      
        1 gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcacgtaa
       61 gaggctaacc tacatgtgtt cagcatggat tgagcctcag attaatgtta agcgtcttgc
      121 aggacgcgta atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaga
      181 cagatagcct caagcacccg cagtcattct acttaactaa gatttccctg caaaccgttt
      241 ttgtccggca cagtaaatat ccaactaaac caatagcgtt cgctgtattt accgccagta
      301 ttcaatgcac atgaccgcca tgaacacccc taaaaaaagg gcatttatat gtccaaacat
      361 taatatcaaa acatcaattt tttccatata ccttgctgtg aagatgatgg gcatacatga
      421 tgcgaacaac cagaacgcaa caaacaaaaa ctgcaatgcg tttttcatta ttccccctac
      481 aatcaatgtg caataacatt taaacacacc tcaatttggc cggacatata aatatctaaa
      541 ccagaaaaaa tcacttacat agcgttacaa actctttagt ctaaatactc atcgtaaaac
      601 attccccata cttatcagtc cgttccgcgc caggtagctt attgccttat ctggcaacct
      661 ataatcaggt ttccgctttt tcagttggct ggtcgtttaa ccgacatagt taacccatta
      721 atctggttgc cggatgctgg tggattttcg cgttttaatt gttcataaaa gtgcacagct
      781 ttaaccagtt cttctgatgt aaccgggact ggtggggcag tgaataaggc ctgaatttca
      841 tagttcggcc tgtcgttaca atcctctttt ttcggtacat atttccagtc accaacccac
      901 aactccccct gagagtccat aacacctttt ttcacgtagc gatatcgcca cgctatcggc
      961 tctgcttcca gcgatgcccg tgcaattttg aataactcgc cctctactcg cgccatccct
     1021 gaattggggt ggcatttcgt aatcgctatt tttaatttgg cttcttcgat taattgttct
     1081 tttgttaatt cagtcatttt cattaccgcc ctggtaatgc tgccaccagc gaaaatggtt
     1141 aatggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg tcagccggga
     1201 tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt ttggtgcgat
     1261 ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaat tacctccaaa tgtataccca
     1321 aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtaacact aaccgccatc
     1381 aaggatggtt tagctgcttt atggaaaaag tatgaggaaa ctgtaaataa tcgcgatcgt
     1441 gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta cagtgacctc
     1501 agtccaagaa cacaaaaaga ttatctgcaa catcaaaaaa agttgcttgc cgtattcggt
     1561 aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga caagagaggg
     1621 gaacagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt ttacagttgg
     1681 gggtatgagc gagggtacgt gaaggctaac ccatgtgcag gtgtaagtaa attcaaggcc
     1741 aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag cgttgcacct
     1801 cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt ttccgatgtg
     1861 ttatcgctga aatgggagca gattggaaac gacgggatct ttatccagca agggaaaaca
     1921 ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga aaaagcaaaa
     1981 cagttaccaa catccgccta tgtaatcagc aatcaatacg gcaaccgata tatgtacaaa
     2041 ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc aggtatttta
     2101 accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga aggaagcagt
     2161 cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat ctatgacagg
     2221 aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc aagaaaatat
     2281 tccaagtaat tccaagtgtg atttttgtca ctgacttaat gatgtataag tgattgaatt
     2341 ttggcggaga gagggggatt tgaacccccg gtggagttgc ccccactccg gttttcgaga
     2401 ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa
     2461 tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt ttgcgagcaa
     2521 aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg
     2581 gcaggaagca ctatcgatcc cccttttaac gtgtctgaca gaacaggaac aaagcaaatt
     2641 ggtcgctctt gccgaacgtt ttttacagca aaaacggctt gttcctttac agggctttga
     2701 gctgaattca ttaagaagct gccggatagc acttctattt tgcctgcccg ttctggagtt
     2761 aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc catttgtggt
     2821 cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg
     2881 tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga
     2941 tgcttctggt tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg
     3001 cgatcgcgcc agcggagttc cctttatttc gttgcgtgag gttgctggct gggaacacga
     3061 tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttagttggtg agaatgcggc
     3121 gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac tttctgaata
     3181 tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg
     3241 tcaattttat caacaagatc ctttgcagag actgcatcac gctaatgata cagactcgtt
     3301 ttcagcgacg aatgttcatt aattaacaac tttgaagatt aattaaccaa ttgaaatggc
     3361 ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgcgccc cgttctcacg
     3421 attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga
     3481 gtccagtcag aggagccaat tttctgtttt catgcatcct tgcgaatctt tatctgatgt
     3541 tgattcaaca ggttagtgtg aaaactcttc tcggtgcatt ttgattttac cctacgcatc
     3601 gggaaaaatt ggtggtcaaa tctgggatca ggttagttcg ataatggagt gacccccata
     3661 tatcccttac cgacgcaaaa atccgcaccc tcaagccttc tgataaaccc tttaaagtct
     3721 ccgattctca cggtctgtat ctgctggtca agccgggtgg ctcccgccac tggtatctca
     3781 aataccgtat tagcggtaaa gaatcccgca ttgcgctggg tgcctatcca gccatctccc
     3841 tgtctgatgc gcgacagcaa cgtgaaggta tccgtaaaat gctggcgctg aatatcaacc
     3901 cggtacagca gcgggctgct gaacgtggct cacgaacacc ggagaaagtt tttaaaaaca
     3961 tggcgctggc gtgacataaa agtaacagga aatggtcgca gaacaccgcc gaccgtctgc
     4021 ttgccagcct gaacaatcac atctttccgg tcatcgggaa cctacctgta tcagaactta
     4081 aaccccgtca tttcattgac ctgctgaaag ggatcgagga aaaaggtctg ctggaggttg
     4141 cgtcccgcac acggcagcac ctgagtaaca taatgcgcca tgcggtccat caggagttaa
     4201 tcgatacgaa ccctgcagca aaccttggcg gcgtgaccac acctcctgtc agacggcact
     4261 atcctgccct gccgctggag cggctgcctg aactgcttga acgtattggg gcatatcatc
     4321 agggccgtga actgacccgg catgccgttc tgctgatgct gcatgtgttc attcgctcca
     4381 gtgaactgcg tttcgcccgc tggtcagaga ttgatttcac aaaccgagtc tggacgatac
     4441 ccgcgacgcg agaacccatt attggcgtgc gttattccgg ccgcggggca aaaatgcgaa
     4501 tgccgcatat cgtccccctc tcagaacagt ccatcgccat tctgaaacag attaaggata
     4561 tcaccggtaa taatgaactg atcttccccg gcgaccataa cccgtataag ccaatgtgtg
     4621 aaaacacggt caataaggca ctgcgggtga tgggttacga cacgaaaaag gatatctgcg
     4681 gtcacggctt ccgggcaatg gcatgcagtg cgctgatgga atcgggttta tgggcaaagg
     4741 acgcagtaga acgccagatg agtcatcagg agcgcaatac cgtgcgcatg gcttatattc
     4801 ataaggcaga gcacctggaa gcccgcaaag cgatgatgca gtggtggtcg gattatctgg
     4861 aagcatgccg agaatcttat gcaccgcctt atacaattgg taaaaataag tttatcccat
     4921 agtatactaa cgaatcccac actctggcaa attaccacca cctctacagg aggtggttct
     4981 atatagactt ttatcaactt catgacgaaa aacacct
//
LOCUS       DAJTOW010000180         4819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTOW010000180 DAJTOW010000000
VERSION     DAJTOW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.181"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(176..760)
                     /gene="kptA"
                     /locus_tag="OO244_003887"
     CDS             complement(176..760)
                     /gene="kptA"
                     /locus_tag="OO244_003887"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5665533.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1010..1693
                     /locus_tag="OO244_003888"
     CDS             1010..1693
                     /locus_tag="OO244_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5665534.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDVAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1690..2151
                     /locus_tag="OO244_003889"
     CDS             1690..2151
                     /locus_tag="OO244_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5665535.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2164..3336
                     /gene="iadA"
                     /locus_tag="OO244_003890"
     CDS             2164..3336
                     /gene="iadA"
                     /locus_tag="OO244_003890"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5665536.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3401..4312
                     /gene="hypT"
                     /locus_tag="OO244_003891"
                     /gene_synonym="qseD"
     CDS             3401..4312
                     /gene="hypT"
                     /locus_tag="OO244_003891"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5665537.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EASFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4305..4697)
                     /gene="iraD"
                     /locus_tag="OO244_003892"
     CDS             complement(4305..4697)
                     /gene="iraD"
                     /locus_tag="OO244_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5665538.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4694..4777)
                     /gene="idlP"
                     /locus_tag="OO244_003893"
     CDS             complement(4694..4777)
                     /gene="idlP"
                     /locus_tag="OO244_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5665539.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1116 a         1273 c         1216 g         1214 t
ORIGIN      
        1 aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt aatttcagca
       61 gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa caaacgctac
      121 attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat tgaatttatt
      181 gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca accgttgatg
      241 tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg
      301 ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt
      361 catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt ttttttatct
      421 cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa
      481 aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac
      541 tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc aatgcacggg
      601 ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc gcccatcctt
      661 cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact
      721 tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt
      781 ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg caactcaatc
      841 ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac gcatagccag
      901 aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag ttccaggaat
      961 tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca
     1021 aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc
     1081 tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca gctggtggcg
     1141 cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaatggagc
     1201 caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc tctttgcgct
     1261 ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg
     1321 tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt tgggcattcc
     1381 agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgtcg ccgccggtat
     1441 gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga ttttcgccgc
     1501 atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt
     1561 tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt
     1621 cggagccaac attttgcgcg tttggcttaa cttcgaagaa cgtcgcaacc caacgcaagg
     1681 agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg
     1741 cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc
     1801 atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc
     1861 gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg
     1921 agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc attaaccggc
     1981 cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt gcaaaatgtc
     2041 ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc
     2101 tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt
     2161 accatgattg attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg
     2221 ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc
     2281 agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag cgggcagatc
     2341 ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg cgaagcaggt
     2401 cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca
     2461 gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag
     2521 acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc
     2581 ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt
     2641 ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct
     2701 aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg
     2761 ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct ggaaaactgc
     2821 gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc gttgtttgag
     2881 caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag cattgacgaa
     2941 ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc
     3001 gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta
     3061 acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa
     3121 gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc
     3181 cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt actggtcatg
     3241 acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc
     3301 aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg ccctaatgga
     3361 cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcggt
     3421 tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa aatgccgcaa
     3481 tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg
     3541 tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc cactgcaact
     3601 ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagaaag
     3661 caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca agatagccgc
     3721 tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc cgccgctctt
     3781 tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc gtgaagggca
     3841 aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgtcgt ttgaccacat
     3901 tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact
     3961 ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact cctacatggt
     4021 gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt
     4081 ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct
     4141 gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc tgaatcggga
     4201 cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca tgaatccagt
     4261 tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct gacattctca
     4321 agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg
     4381 tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc ttctttcaaa
     4441 ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg
     4501 caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc gggcatgcct
     4561 gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag gagatcgctg
     4621 cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga
     4681 agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg
     4741 aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt tgttcattac
     4801 atccatgtca gtctgagac
//
LOCUS       DAJTOW010000181         4669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTOW010000181 DAJTOW010000000
VERSION     DAJTOW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.182"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..527
                     /locus_tag="OO244_003894"
     CDS             <1..527
                     /locus_tag="OO244_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5665540.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="OO244_003895"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="OO244_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5665541.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="OO244_003896"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="OO244_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5665542.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="OO244_003897"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="OO244_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5665543.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="OO244_003898"
     CDS             4170..4343
                     /locus_tag="OO244_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665544.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="OO244_003899"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="OO244_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5665545.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1193 a         1077 c         1176 g         1223 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccaca cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaa
//
LOCUS       DAJTOW010000182         4642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTOW010000182 DAJTOW010000000
VERSION     DAJTOW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.183"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..312
                     /locus_tag="OO244_003900"
     CDS             <1..312
                     /locus_tag="OO244_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5665546.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            469..1218
                     /locus_tag="OO244_003901"
     CDS             469..1218
                     /locus_tag="OO244_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5665547.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1240..1806
                     /gene="yieF"
                     /locus_tag="OO244_003902"
                     /gene_synonym="chrR"
     CDS             1240..1806
                     /gene="yieF"
                     /locus_tag="OO244_003902"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5665548.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="OO244_003903"
     CDS             complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="OO244_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5665549.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3362..4018
                     /gene="yieH"
                     /locus_tag="OO244_003904"
     CDS             3362..4018
                     /gene="yieH"
                     /locus_tag="OO244_003904"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5665550.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4085..4558
                     /locus_tag="OO244_003905"
     CDS             4085..4558
                     /locus_tag="OO244_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5665551.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1242 a         1163 c         1138 g         1099 t
ORIGIN      
        1 gcactggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
       61 gaattcgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
      121 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
      181 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
      241 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
      301 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
      361 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
      421 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
      481 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
      541 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
      601 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
      661 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
      721 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
      781 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
      841 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
      901 attacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
      961 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     1021 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa
     1081 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
     1141 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     1201 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
     1261 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     1321 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     1381 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     1441 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     1501 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     1561 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     1621 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     1681 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     1741 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     1801 atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt
     1861 taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     1921 gggctaaggt cacgcagacg tccggtacca atcttcatca cgcagtagga gataaagccc
     1981 agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc
     2041 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
     2101 ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca ccattcccgc cagcggcgac
     2161 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     2221 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     2281 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc
     2341 cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     2401 aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     2461 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     2521 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     2581 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga
     2641 agtacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgagtt agcgacaatc
     2701 acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc
     2761 acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa
     2821 agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc
     2881 attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc
     2941 accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt
     3001 gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat
     3061 acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta
     3121 gtaccatgtt cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg
     3181 gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     3241 accaaaatgc gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg
     3301 caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg
     3361 catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagtgaagt
     3421 catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc tcgatcctga
     3481 agaggtattc aaacgtttca aaggtgtaaa actgtacgaa attatcgata ttgtttccct
     3541 tgaacatggt gttacgttag cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc
     3601 tcggctgttc gattcagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc
     3661 aatgtgtgtg gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa
     3721 tatgttgcac tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc
     3781 agacccggcg ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat
     3841 tctggttgat gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt
     3901 ctacttctgc gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac
     3961 ccatctttcg cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt
     4021 cttcacactc ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt
     4081 tgatatgtct gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc
     4141 gttaattggt gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct
     4201 tttggtaata atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat
     4261 tcctgcattg ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa
     4321 aaattatcgt tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc
     4381 agttattgta catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg
     4441 tgacagtctc gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat
     4501 cattacccgt ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc
     4561 gggatacttt atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat
     4621 caggccgtta ccccaattca aa
//
LOCUS       DAJTOW010000183         4469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTOW010000183 DAJTOW010000000
VERSION     DAJTOW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.184"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(91..627)
                     /gene="ssb1"
                     /locus_tag="OO244_003906"
     CDS             complement(91..627)
                     /gene="ssb1"
                     /locus_tag="OO244_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5665552.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            881..3703
                     /gene="uvrA"
                     /locus_tag="OO244_003907"
     CDS             881..3703
                     /gene="uvrA"
                     /locus_tag="OO244_003907"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5665553.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3738..4094)
                     /locus_tag="OO244_003908"
     CDS             complement(3738..4094)
                     /locus_tag="OO244_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5665554.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4098..>4469)
                     /locus_tag="OO244_003909"
     CDS             complement(4098..>4469)
                     /locus_tag="OO244_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5665555.1"
                     /translation="FHLVTDEILNQLADMPRVNIGLLHLLLQHTSASLTLNENCDPTV
                     RHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVLPVHKGRIQTGTWQGI
                     WLGEHRIHGGSRRIIATLQGE"
BASE COUNT         1023 a         1217 c         1196 g         1033 t
ORIGIN      
        1 agcctccata acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa
       61 aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat
      121 cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc
      181 gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct gcggctgacc
      241 accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca gcatctgcat
      301 ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg attgatcggt
      361 ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc
      421 gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgttcttt
      481 catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac
      541 tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat
      601 aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata
      661 agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt gacctcggtt
      721 ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc attccaatac
      781 tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc
      841 atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga tcgaagttcg
      901 gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg acaagctcat
      961 tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca ccttatatgc
     1021 cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt ttctgtcact
     1081 gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct caattgagca
     1141 gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa tccacgacta
     1201 tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg acgtcccgct
     1261 ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg aaggcaagcg
     1321 tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca aaacgctgga
     1381 gaacttggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct gcgatctttc
     1441 cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt
     1501 caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg cgctggaact
     1561 ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag agctgctgtt
     1621 ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg agccgcgact
     1681 gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg gcgtacagca
     1741 atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg gcggtgcgat
     1801 ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc tggcagatca
     1861 ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt
     1921 gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc gtggcgatac
     1981 ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc gttataaaga
     2041 gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc gcccatgcgc
     2101 cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc
     2161 gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca acaatctcaa
     2221 actcgctggt cagcgggcga agattgcgga aaaaattctt aaagagatcg gcgatcgcct
     2281 gaaattcctc gtaaacgtcg gcctgaatta cctgacactt tcccgctcgg cagaaacact
     2341 ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg gcctggttgg
     2401 cgttatgtac gtgctggacg agccgtctat cggcctgcac cagcgcgata acgagcgcct
     2461 gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg tggagcatga
     2521 cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca
     2581 cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc ctgaatcgtt
     2641 gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc
     2701 gaatccagaa aaagtgctga agctgacagg cgcacgcggc aacaacctga aggacgtgac
     2761 gctcacgctg ccagtcggtc tatttacctg catcacaggg gtttcaggtt ccggtaaatc
     2821 gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat
     2881 cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata aagtgatcga
     2941 tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct ataccggcgt
     3001 gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc gtggttatac
     3061 gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc agggcgacgg
     3121 tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg atcagtgcaa
     3181 aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa ccatccacga
     3241 agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc cagctctggc
     3301 gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg ggcagtccgc
     3361 aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg
     3421 cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc acttcgccga
     3481 tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata ccattgtggt
     3541 gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc tgggaccaga
     3601 aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg
     3661 cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa ggccgctttc
     3721 tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc cggtagtaaa
     3781 ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc cggcagcgaa
     3841 ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg acgcacatcg
     3901 ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga aacagctggg
     3961 cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat ctgcgtcgct
     4021 ttccagtcgt tatgcacgct ctgttctgcg cttggttttg ccatgcaata ttgtagcaac
     4081 tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg
     4141 tggatgcgat gttcccccag ccagatgcct tgccaagtgc cggtctgaat acgcccttta
     4201 tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga aggcatatcg
     4261 tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa
     4321 cactccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag agaggcggag
     4381 gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc agccagctga
     4441 ttcagaattt catccgttac cagatgaaa
//
LOCUS       DAJTOW010000184         4384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTOW010000184 DAJTOW010000000
VERSION     DAJTOW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.186"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            19..639
                     /locus_tag="OO244_003910"
     CDS             19..639
                     /locus_tag="OO244_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5665556.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(648..1955)
                     /gene="ynjE"
                     /locus_tag="OO244_003911"
                     /pseudo
     CDS             complement(648..1955)
                     /gene="ynjE"
                     /locus_tag="OO244_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2022..2675)
                     /locus_tag="OO244_003912"
     CDS             complement(2022..2675)
                     /locus_tag="OO244_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5665557.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2675..4146)
                     /locus_tag="OO244_003913"
                     /pseudo
     CDS             complement(2675..4146)
                     /locus_tag="OO244_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(4119..>4384)
                     /locus_tag="OO244_003914"
     CDS             complement(4119..>4384)
                     /locus_tag="OO244_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5665558.1"
                     /translation="TIPANANASAAAKVVANFLLSPDAQLRKADPVVWGDPSVLDPQK
                     LPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
BASE COUNT         1008 a         1200 c         1169 g         1007 t
ORIGIN      
        1 tatatttaag gagtgcctgt gctagaccgc catcttcatc cccggattaa accgttgttg
       61 catcagtgcg tgcgggttct tgataaaccg ggcattacgc cagatggttt aacattagtg
      121 ggatttgcca tcggcgtgct ggctctgccg tttctggcgc tgggctggta tctggcggcg
      181 ctagtcgtca ttttgttgaa caggctgctt gatggtctgg acggtgcgct ggcgcggcgc
      241 agagggctta ccgacgcggg cggctttctt gatatttctc tcgattttct cttttacgcg
      301 ctggtgccgt ttggctttat tcttgctgca ccggagcaaa atgcgctggc aggcggctgg
      361 ttgttgtttg cgtttatcgg cacgggcagc agttttctcg cctttgctgc actggcagca
      421 aaacatcaga ttgataaccc cggttatgca cataagtcgt tttactatct ggggggatta
      481 accgaaggca cagaaacgat cttactgttt gtgctgggat gtttattccc tgcatggttt
      541 gcgtggtttg cgtggatctt cggtgcgttg tgctggatga cgacctttac gcgggtgtgg
      601 agtgggtatc tgacgctgaa gtcgctccag cgtcagtaat tgcaatgtta tttgctactg
      661 cccgggccgc gttcaccggt tgcaaccgga ttttttggat cgctgctcca ttcgtaccag
      721 ccgccgtcat acacggaaac attcttccaa cccatggcgc gtgcgtacat aaaggtttcg
      781 gacgcgcgcc agccggtgcc gcagtagaat gaaacttgct gatctggttt gatattccat
      841 gctttccaca tagcggtaat atcatcggcg ctacgcatgg tgccatccgg gttatagaaa
      901 tcttccatat gcgtcgagtc gctaccagcg tgtccccaac gtgctccggc tatttcacct
      961 tttggtttaa tatagctgta accgctggtc gtaccgataa attctggcca cgaacgaatg
     1021 ctcaccagcg atgcatcctg gcgatgcagc agtccacggg cctgttccat atcgagcatc
     1081 aactgcggtt gtgccgggat cttcacgccg aaatccggtt ccgctttcac tttcggtggc
     1141 gttcctcgct caacaggcag tcccgcgtcg gaccaggttt gccagccgcc atccagcaga
     1201 cgcacatctt tcacgcctgc gtagagcata atttgcgcca cacgcgctcc agcgtatacg
     1261 tcacgcccgt acagaatgac cgtggtgtca tggcgaatgc cgtgttttgc cagcatcgct
     1321 tttagttgtt catcagaaac tttgttccac agcggttcgc tttccacttc gttggtatcg
     1381 atgtagtcag cgccaggaat atggctgata aggtaaagct taggagcgcc ctaggccgct
     1441 tcaatgactt tccagtcacc gacaggtttc gccgtaacct ctttaccttg ttgcaggtca
     1501 tgcagccatt gcggataaac cagctgctca aagtgcggca gtttttgtag acgggaaggt
     1561 tcgcttagcg cgtcactcag aatggagata tgcgtaaaac ctgctttttg cagtcgcgtt
     1621 ttgacggcgt caacatcttt gccattaccg tacagcgcca ccggggcatc ggctttcagg
     1681 ttatgttgct tgatccacgc gttgagctgt tcggtgctca ttttgtcgag ccagctagca
     1741 gagaggttta aggcggcagg ttcatgacca gaagggccat ttaaggtttg tggccagccg
     1801 ttataaaacg cgctggggcg agtatctatc gctttgccat tttgttgctg aagttggtca
     1861 agtgtaagag gcttcgccag ttcagcggcc cacgaagaag cgcaagccag ccctaaagcc
     1921 attgccagcg cggtcatttg agaaacacgt ttcatcgaaa atcccgacat tgtagttttg
     1981 agtgcgccat tctgaactga cgcattgaga aaaactttgc gttatcgcag tttgttgtaa
     2041 ttttctgacc actgcgccat atccagaaca gaactatcag caggaacatc ctggagatcg
     2101 tgcgttacct gaacgacggg gatcgccagg gcgcgaactt cgctgaacac ccactggcga
     2161 aaattatcgc gcagagccac atcaagacgg ctgaatggct catccaggag taacgctttt
     2221 ggctgggcga gaagggcgcg tagtagagca acgcgcgctc gctgaccgcc agacaaagtg
     2281 gcaggatcct gatggaaagc tccgtcaagg cctgaacgct caagtgcatc attcacggca
     2341 ttacgtcggg cattcccctt aagtgtcgcc ggtagcgcca gcagtaaatt ttgcccgacg
     2401 ctgaactggt caaataacag tgcatcctga aaaagaatgc caatctgacg ctgtgcggtg
     2461 ggtagcatgt caatccgttg ctcattgagc catagctcac ctgtacaaga aaactgtccg
     2521 gccagtgcac caatcatcca tgaaaacaga gtggattttc cacagccaga cggccccatt
     2581 aacgtgacga tgtcaccttt atcaaccgta aagttaacgt ttgtcagcaa gcggctttct
     2641 ggtaaacgta gcgaaacatt tttcacgcag agcattagcg gattccttgt ctgacataac
     2701 ctacccattt tgcgactaac gcggtcaggg caaaaataat aagcggtaac aacagttgcc
     2761 ataacgcctg ggcggcgaga ataccgttgc tgccgccgct gcttaatgcc accgcctcag
     2821 tggtgagcgt cggaaaacgc cccgcgccca gccacagcgt cggcatatac tgcgcaatac
     2881 tgactgaaaa tcccaccgcg aaggcaatca gcacagggcg caacatgagt gggcatttca
     2941 cgtaaaagaa gattttggcc cgcgaccagc ccagtgtttg cgcaatcaaa attaaccgtg
     3001 aatcaatgcg ctgccaggcg ggttgcagga taaacagcat ccacggcatt acccacagca
     3061 gatgccccca gaccaccgcc gtccagcttc catccaggtt cagccatagc gccagcgtgt
     3121 actggcccgc caccagtggc agagcgggca atagaatggg caaccatagc cacaactggc
     3181 gacgctgtgc cccccattcc agccacagca gtaacaggag caaagcgatg aatgttgcta
     3241 ccagccccat tgtcagactg ttgatgagtg cttcactatt gatcgtcgac tgatccgcga
     3301 ggatcgccag cagaaccaca cacagcacac cggttaaggg taaaaaactc gccagcgtag
     3361 tgcctggtaa taaaggtgtg gcaggcttgc gaatgccatc tacgcgggga atagtgcgcc
     3421 gccagctgcg ccaaaacgta ggcggcgagt aacagcatca gcagcagact ggcgagtgcg
     3481 cctttcgttt gttgatccgc gtcgccctga gttaaccact gccagctaat tactgccagc
     3541 gtcggtggat tacctggccc aagaataatc gccacatcca cgaccgaaag cgaccaggca
     3601 acaatcgcca gcatcgccat tgccagcggc tgtagcccag cgaatccagc acaatgacct
     3661 gctgcaacag ccgtttttcg ctcaatactg ccactaagat ccacagcaga aatgcgcttt
     3721 ctttcactgc aagggtgagg cccagaccga tccaaatcgg tccattggcg gagtgaaata
     3781 cgggaaatag tcatatagca gccctccgtc agcaaagagc agcagggcgc tggtggcaaa
     3841 agccacatgg ggaatggcga gcagccacgg cagacgggcg cacatacgct gccatttcgg
     3901 cccaggccat agcgccacaa tcaccagcag ggcaatcaac aatgccccga ccgccgcgat
     3961 ggttgtcgac accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc
     4021 ctgccagcgt gtcaacgaca acgcaggcga tatcagcgtg agagctgccg ggatcagcgg
     4081 tgcataaatc accgccacca tcgcccacag caaaaagatt aatgcgtacc gtaacggcgt
     4141 aaccattctt gttccagtgc attcacccat cctgcgtgcg gttcagccag taccggcggc
     4201 agatcctgcg gcattcttga ttgcaatatt tcgcgctgcc cgtcaggcag tttttgcgga
     4261 tcgagaacag aaggatcgcc ccagacaacg ggatctgctt tacgcagttg cgcatcgggt
     4321 gagagcagga aattggcaac taccttcgcc gcagcactgg cattcgcgtt ggctggaatg
     4381 gtga
//
LOCUS       DAJTOW010000185         4319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTOW010000185 DAJTOW010000000
VERSION     DAJTOW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.187"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO244_003915"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO244_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5665559.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO244_003916"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO244_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5665560.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO244_003917"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO244_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5665561.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO244_003918"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO244_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5665562.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4144..>4319)
                     /locus_tag="OO244_003919"
     CDS             complement(4144..>4319)
                     /locus_tag="OO244_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5665563.1"
                     /translation="YHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDM
                     NDEQIAMLNARLS"
BASE COUNT         1163 a         1050 c         1034 g         1072 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataaccta
     3781 tacaaaatga aaataattat cattaacagc tttgaggcct aaaacaggga ttaggttgtt
     3841 tcctgctggc gacatgatat tgaggaaaaa agtagagcag ctattaggct ttgaaatgac
     3901 tttatgagga aaattgttga tggcaaaaat ggggcaaaaa agagtgggtt atcgtagctt
     3961 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgagtcaga
     4021 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttctttg taatttgttg
     4081 attttcttgc attatttcag ttctctggta ctaaatgggg caaattgggg gcaaactttg
     4141 caactacgat aaccgcgcat tcaacatggc tatctgttcg tcgttcatgt catcaatcca
     4201 cataccgtaa atttcataca ccatttgcgc agtttcatgc cccatctggc tggctataaa
     4261 tgccgggttc gctcctgccg tcaacagcca gcaggcaaaa gtatgtcgcg tatggtacg
//
LOCUS       DAJTOW010000186         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTOW010000186 DAJTOW010000000
VERSION     DAJTOW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.188"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO244_003920"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO244_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5665564.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO244_003921"
     tRNA            complement(324..399)
                     /locus_tag="OO244_003921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO244_003922"
     tRNA            complement(406..480)
                     /locus_tag="OO244_003922"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO244_003923"
     tRNA            complement(597..681)
                     /locus_tag="OO244_003923"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO244_003924"
     tRNA            complement(690..765)
                     /locus_tag="OO244_003924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO244_003925"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO244_003925"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5665565.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO244_003926"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO244_003926"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5665566.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO244_003927"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO244_003927"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5665567.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1090 a         1046 c          936 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg agaagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTOW010000187         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTOW010000187 DAJTOW010000000
VERSION     DAJTOW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.189"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            84..752
                     /locus_tag="OO244_003928"
     CDS             84..752
                     /locus_tag="OO244_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665568.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO244_003929"
     CDS             complement(995..1690)
                     /locus_tag="OO244_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5665569.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO244_003930"
     CDS             complement(1683..3110)
                     /locus_tag="OO244_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5665570.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO244_003931"
     CDS             complement(3121..3840)
                     /locus_tag="OO244_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5665571.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1120 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcggaa
//
LOCUS       DAJTOW010000188         4184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTOW010000188 DAJTOW010000000
VERSION     DAJTOW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.190"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(349..996)
                     /gene="ychE"
                     /locus_tag="OO244_003932"
     CDS             complement(349..996)
                     /gene="ychE"
                     /locus_tag="OO244_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5665572.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1473..4148
                     /gene="adhE"
                     /locus_tag="OO244_003933"
                     /gene_synonym="adhC"
     CDS             1473..4148
                     /gene="adhE"
                     /locus_tag="OO244_003933"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5665573.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1034 t
ORIGIN      
        1 caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac agttccagta
       61 gtgaaatgca caataattta acaattgatt attatttagc actttcctct accaaaggca
      121 gtggaattac taatatcatt tcaattattc ttcagcaagc ccaggattat gatgtggcga
      181 aaataacatg atggtgagga gaagcagtta aggaaatcct tataactaac tgtaatttga
      241 gaagaatatt tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat
      301 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
      361 gccggggaaa atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa
      421 tagccccata atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca
      481 cggtgccatg cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa
      541 accaaacaga tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc
      601 ccctggcccc gccatcaacg gcaacgccaa tggcaccaca ccaatacttt cacgtaccgc
      661 ggtttctgat ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga
      721 catcgctatc gtcaccacca ggatgccccc ggcgatacgg aacgaatcaa ttgatatacc
      781 aaaaagttgt agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga
      841 caggttggct gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct
      901 gataaagacg ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt
      961 gaagtaaacg ggaaaatcaa aaagggtctg aatcacggtt cgctccgaag caaaagccgg
     1021 ataatgttag ccataaataa ggttgaaaag acgcgctgac aatacgcctt ttgacagcat
     1081 ttttcacctc ctaactactt aaaattgcta tcattcgtta ttgttatcta gttgtgcaaa
     1141 acatgctaat gtagccacca aatcatacta caatttatta actgttagct ataatggcga
     1201 aaagcgatgc tgaaaggtgt cagctttgca aaaatttgat ttggatcacg taatcagtac
     1261 ccagaagtga gtaatcttgc ttacgccacc tggaagtgac gcattagaga taataactct
     1321 aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt
     1381 catcggttaa gcaaatcatc accgcactga ctatactctc gtattcgagc agatgattta
     1441 ctaaaaaagt ttaacattat caggagagca ttatggctgt tactaatgtc gctgaactta
     1501 acgcactcgt agagcgtgta aaaaaagccc agcgtgaata tgccagtttc actcaagagc
     1561 aagtagacaa aatcttccgc gccgccgctc tggctgctgc agatgctcga atcccactcg
     1621 cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga agataaagtg atcaaaaacc
     1681 actttgcttc tgaatatatc tacaacgcct ataaagatga aaaaacctgt ggtgttctgt
     1741 ctgaagacga cacttttggt accatcacta tcgctgaacc aatcggtatt atttgcggta
     1801 tcgttccgac cactaacccg acttcaactg ctatcttcaa atcgctgatc agcctgaaga
     1861 cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa agatgcaacc aacaaagcgg
     1921 ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc gaaagatctg atcggctgga
     1981 tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca ccacccagac atcaacctga
     2041 tcctcgcgac tggtggtccg ggcatggtta aagccgcata cagctccggt aaaccagcta
     2101 tcggtgtagg cgcgggtaac actccagttg ttattgatga aactgctgat atcaaacgtg
     2161 cagttgcatc tgtactgatg tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac
     2221 agtctgttgt tgttgttgac tctgtttatg acgcagtacg tgaacgtttc gcaacccacg
     2281 gcggctatct gttgcagggt aaagagctga aagctgttca ggatgttatc ctgaaaaatg
     2341 gtgcgctgaa cgcggctatc gttggtcagc cagcctataa aattgctgaa ctggcaggct
     2401 tctctgtacc agaaaacacc aagattctga tcggtgaagt gaccgttgtt gatgaaagcg
     2461 aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat gtaccgcgct aaagatttcg
     2521 aagacgcggt agaaaaagca gagaaactgg ttgctatggg cggtatcggt catacctctt
     2581 gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga
     2641 aaacagctcg tatcctgatt aacaccccgg cttctcaggg tggtatcggt gacctgtata
     2701 acttcaaact cgcaccttcc ctgactctgg gttgtggttc ctggggtggt aactccatct
     2761 ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac cgttgctaag cgagctgaaa
     2821 acatgttgtg gcacaaactt ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg
     2881 cgctggatga agtgattact gatggccaca aacgtgcgct catcgtgact gaccgcttcc
     2941 tgttcaacaa tggttatgct gatcagatca cttccgtact gaaagcagca ggcgttgaaa
     3001 ctgaagtctt cttcgaagta gaagcggacc cgaccctgag catcgttcgt aaaggtgcag
     3061 aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct gggtggtggt tccccgatgg
     3121 acgccgcgaa gatcatgtgg gttatgtacg aacatccgga aactcacttc gaagagctgg
     3181 cgctgcgctt tatggatatc cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag
     3241 cgaaaatgat cgctgtcacc accacttctg gtacaggttc tgaagtcact ccgtttgcgg
     3301 ttgtaactga cgacgctact ggtcagaaat acccgctggc ggactatgcg ctgaccccgg
     3361 atatggcgat tgtcgacgcc aacctggtta tggacatgcc gaagtccctg tgtgctttcg
     3421 gtggtctgga cgcagtaact cacgccatgg aagcttatgt ttctgtactg gcatctgagt
     3481 tctctgatgg tcaggctctg caggcactga aactgctgaa agaatatctg ccagcgtcct
     3541 accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt tcacagtgca gcaactatcg
     3601 cgggtatcgc atttgcgaac gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg
     3661 gttcccagtt ccatattccg cacggtctgg caaacgccct gctgatttgt aacgttattc
     3721 gctacaatgc gaacgacaac ccgaccaagc agactgcatt cagccagtat gaccgtccgc
     3781 aggctcgccg tcgttatgct gaaattgccg accacctggg tctgagcgca ccgggcgacc
     3841 gtactgctgc taagatcgag aaactgctgg catggctgga aacgctgaaa gctgaactgg
     3901 gtattccgaa atctatccgt gaagctggcg ttcaggaagc agacttcctg gcgaacgtgg
     3961 ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg cgctaacccg cgttacccgc
     4021 tgatctccga gctgaaacag attctgctgg atacctacta cggtcgtgat tatgtagaag
     4081 atgaaactgc agcgaaaaaa gaagccgctc cggctaaagc tgagaaaaaa gcgaaaaaat
     4141 ccgcttaatc agtagcgctg tctggcaata taaacggccc cttc
//
LOCUS       DAJTOW010000189         4168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTOW010000189 DAJTOW010000000
VERSION     DAJTOW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.191"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(33..212)
                     /locus_tag="OO244_003934"
     CDS             complement(33..212)
                     /locus_tag="OO244_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5665574.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTEVYWQ"
     gene            complement(362..523)
                     /locus_tag="OO244_003935"
     CDS             complement(362..523)
                     /locus_tag="OO244_003935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665575.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(520..708)
                     /locus_tag="OO244_003936"
     CDS             complement(520..708)
                     /locus_tag="OO244_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5665576.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            968..1303
                     /gene="iraM"
                     /locus_tag="OO244_003937"
     CDS             968..1303
                     /gene="iraM"
                     /locus_tag="OO244_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5665577.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1584..1692)
                     /locus_tag="OO244_003938"
                     /pseudo
     CDS             complement(1584..1692)
                     /locus_tag="OO244_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2215..2291)
                     /locus_tag="OO244_003939"
     tRNA            complement(2215..2291)
                     /locus_tag="OO244_003939"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2255..2257),aa:Arg,seq:tct)
     gene            complement(2393..2468)
                     /locus_tag="OO244_003940"
     tRNA            complement(2393..2468)
                     /locus_tag="OO244_003940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2433..2435),aa:Ile,seq:cat)
     gene            complement(2613..3434)
                     /locus_tag="OO244_003941"
     CDS             complement(2613..3434)
                     /locus_tag="OO244_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5665578.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3449..3805)
                     /locus_tag="OO244_003942"
     CDS             complement(3449..3805)
                     /locus_tag="OO244_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5665579.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3818..>4168)
                     /locus_tag="OO244_003943"
     CDS             complement(3818..>4168)
                     /locus_tag="OO244_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5665580.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1173 a         1129 c          803 g         1063 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc agtcagatat
       61 gtcagcagcc caaacagcag actccccagc acgcctattg ccgcccactg agacggggaa
      121 accctgtcca gcaactgcag gaaccagtag cccgttccca ccgctgacgt ggtgtatgac
      181 acacctgttg tgattttttc catctggtcc ataccccgtc tcccgttatc cggaagctga
      241 caacaataaa aaagccacca gttaactact gatggctctg ataactcatg caagcgtctc
      301 agacgaccca ctgacactac cggtgagttt aacgatacct tccatttgac tggctcactt
      361 tttatgatga tgccggtgca tttatctcca gcaccagact ttctatctca acgccatacg
      421 ttgcattttt ggtaatatcc gtcagcgtca gtgcatttag tcccactgcc agactgtctt
      481 ttatggcctg gaatgccggg ccagtacgat gacgtagtat cactccggct cagttgcacc
      541 actgaccacc acatcacctt ctgctgcaat cgcctgcatc agggtataag gggttatggc
      601 caccggacta ccaaacggct gccagccctc tttcagttta tgtgtcagct tttccgcaag
      661 atctgacggc ggcgccgccc tgacaacatc atagtgttta aatgccatgg ttctttccaa
      721 catctgaaaa ataattcttt aaaatacctg acatgtaata cagaaaaaac acaaaaccat
      781 accttaaata aaaacctgat tatcaagcag atatgcatgg ataaactaca agacgagata
      841 taaaccaccc tgcatttaaa taaacaataa acaacatcag aaaaataatt ctgttctatg
      901 gtttaattca aaaatatcat ttatactttt cagaacatca ccagcaaggc ataaacaagg
      961 aaactaaatg aagtggattg tgattgatac agttatccag ccatcatgcg gaatatcttt
     1021 ttcagtcata tggagtaaaa taaaattaat aatctggtat caatcggatg ctttcttacc
     1081 tcctgaaagt atatttacac tgactcacac aggcatcatg ctcaataaca aagtgctacc
     1141 tgtaaccatt tacaacgtag taccattcaa taaaacattc tggaatttaa tcaaaaacag
     1201 ccaggaatgc cctacaaata cagataacgt attgaatgaa tgctttaata accgttgcac
     1261 tctgcaaata tgtccttatg gactaaaaca acaaagtcca taaggagttt actcacatct
     1321 gacaaaatca atataaacag cccctccgga gaggggctgg agagtggcgc tatgtgccat
     1381 tccatggtgc cgggtgcctc ccggtgaatt cagtaccagc acctgaatcc gcgattatcc
     1441 catataccta ctcgctgatt gcccctccgc acagggggat tcaccatgcc agtttctttt
     1501 aacaaactcc ccgcaaacca gacaacagtc aaccgcctga attgtgaagt atttaaaaat
     1561 ttctcccgct aactgatacc cggctaacag tctggcgttt tctttttcag caacgggaaa
     1621 gcaacaacca ccacacccgc caccagcaca ccgtcagccg gcactgacat tatccggctg
     1681 ctgcaatgcc attaacaaaa acagtaagca atcacttttt accgtaacag gtgataatcc
     1741 agatatgtat ctaccccaga tgagtaatcc gaagttcatc cataccacag gtcctggcta
     1801 ttctgttgta ctcctgaaca agagcaaata attctgaatt agcaaccatg aactcatcgc
     1861 aaaccctctg tatagcatca ctattcagaa taataacgtc tcttcccgaa agacgatcag
     1921 gagtacagaa caaaactgtc aaacggctga aggcctttgc tcgtgctgca ttgactatat
     1981 caatacgctg cctaaggatg aaacaccccg acgcctcatc aatattcact ctacccacac
     2041 catatgaatg ataaatactt aatgctgaaa aaaccattag accgtataac aaatactcaa
     2101 tcaatactta acagaacttt tatttttgac aaatatataa tattttcaac aatatcctga
     2161 gccaggtata tttcagtata aggctctgcc ggaaggaatc tggaagaatg aatatggcgc
     2221 gctgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct gtccggctga
     2281 gctaacaacg ctgaataccg ataatggacc gccatcgggg acccgccccc cgcaccaaca
     2341 accctgttat cgtgtcgtct gctcttcctg ataagctaat ggcggtttgt gatggtggcc
     2401 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
     2461 aaagggccgg gagcagaata ataacggtcc gtaattaatt tcgcaataaa aaaacccgct
     2521 cagcggcggg ttgtagaaac tcttctaacg ccaggcataa aacgcccatt attatgacga
     2581 atttaccaca gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc
     2641 tcagcccatg cttcttcaat atcaaaccgg gtcaccagcg catcataaaa tttcttaact
     2701 gttttttccc atgacgcgcg tgttatctgg tttgtcacct cgcatatagc attaaatgcc
     2761 tccgttgatg gtagtctttc atagccacga ccaccacaac gctggcagtc tctgataaca
     2821 ggcataccac gttttaccga ctcttcacga tgaatggcga caccacgccc acggcaatcc
     2881 ttacaggcgg tggaaacctc accctttccg ccacactccg gacaggcaac ttttaccacc
     2941 tccctgactt ttttccattc ttcccagtaa aacggataca cacctttcgt acactttgcc
     3001 cataccggcg gcttaccatc cggatactgg atcttgtttg taaaaactat gctttcaata
     3061 aatttttccc catagcaaca agggcactgc tttttactcg ctgcgctgcg ggcataatcc
     3121 tcaaaagcgt acgaagccat aatgcgcatc actaccggtt ttatttctgc cggaagtttt
     3181 ctcaacgccg ccacacgatc gcaccgactg agtgcataat ctgccagtaa ttctgttgcc
     3241 cgcgccctgt cattcatact gatgcccatt ttccccagga acgcagaaaa acccatctca
     3301 gcccgattct gtgtcatgcc ctgcgcggcc atcacatcag tgatactcag cgcatctttt
     3361 gacgttgagg ccgatgcatc ggtcaggcca ggggattttg gggagtagta tttcggtaaa
     3421 tcttccagtt tcattttttg acctgctctt aatgcattat ggggtaaatc ttcaccccca
     3481 gacgtccacc agatactggc tgaccacgaa cgatattgat ttcatcaaac tgctcatcgt
     3541 ccattaacac tcccgcatgc gtcagcgcat ccagcggtgc tttcaggata ttgtccaggt
     3601 cgcgacgacg cttatccggt ggctctgcaa tcacctttat cgccagcctt ccggacaggc
     3661 ttaatttcag ccgctgctgg cgaacaataa gcgccacagc ccggcgataa cgctttccct
     3721 cctccgagat aaaatatgtg ctgccacggc gtcgccagta agtgttcacc gtcggcgggt
     3781 aaggtaaaac caaatctatg agcatcagtc acctctttta cccaagcacg ccagttgcaa
     3841 aggcgtgatc aagaaaacga aaaattaaat caacctgaga accatgcttt tcttcgaacg
     3901 ccagaggatc cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc gtgaaaatat
     3961 cgtgagattt ggtccccatt ccgccctgac catgaccaat caggtgatgg ggatcgtcgg
     4021 ctggcttacc acaacacgca cacggctgtg tcttcaccca gcgtttgtat ttctcgttaa
     4081 cccagcggcg acgtttaggt cgtttcatga aagattccgg agactcagga tcaacggcaa
     4141 tgctgaccac cgtcttttcc tgttgtgg
//
LOCUS       DAJTOW010000190         4101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTOW010000190 DAJTOW010000000
VERSION     DAJTOW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.192"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..105)
                     /locus_tag="OO244_003944"
     CDS             complement(<1..105)
                     /locus_tag="OO244_003944"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5665581.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKG"
     gene            complement(246..2279)
                     /gene="metG"
                     /locus_tag="OO244_003945"
     CDS             complement(246..2279)
                     /gene="metG"
                     /locus_tag="OO244_003945"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5665582.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2411..3520
                     /gene="apbC"
                     /locus_tag="OO244_003946"
     CDS             2411..3520
                     /gene="apbC"
                     /locus_tag="OO244_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5665583.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3782..4063
                     /locus_tag="OO244_003947"
     CDS             3782..4063
                     /locus_tag="OO244_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5665584.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1002 a         1017 c         1051 g         1031 t
ORIGIN      
        1 ccctttggtg ccaacttttc cccaactgat atgcaactcg tttccctgtt gctccaccgt
       61 ccagaattta tgtgattttt cgtcctgata gataaagtgt ctcattctgc atccctataa
      121 ttttatccag ttgaatgcag atgctaccag tatttatacg ggttagagag agacaaaatc
      181 gcaagaaaac aactttagga atttataaag aaaaggcgct gtcgatgcag cgccttgaag
      241 ggggattatt tcacctgatg acccggttta gcaccggcat ccgggcttag caggaaaata
      301 tctttcccgc caggaccggc agccatcacc atgccttcag agataccgaa gcgcatttta
      361 cgtggtgcca ggttagccac cataatggtg tgacgaccaa tcagtgcctg cggatccggg
      421 taagcagaac gaataccgga gaagacattg cgtttttcac cgccgagatc cagcgtcagg
      481 cgcagcagtt tgtcagaacc ttcaacaaac tctgcgtttt cgatcagcgc cacgcgcagg
      541 tcaactttgg cgaagtcgtc aaaggtgatg gtttcctgaa tcggatcatc tgccagtggg
      601 ccagtaactg gcgcggtagt ggctttcact tcttctttag aggcttccac cagagcttca
      661 acctgtttca tatcgatgcg gttatacagc gccttgaacg gattcacttt gtggcccagc
      721 agcggttgct ggataccatc ccaggtcagt tccgtattga ggaaggcttc tgcacgctcg
      781 gtcagtttcg gcagtacagg cttcaggtaa gtcatcagca cgcggaacag gttgatgccc
      841 attgagcaaa tcgcctgcag gtcagcatcg cgaccttcct gttttgcgac tacccacgga
      901 gcctgctcgt cgacatagcg gttagccagg tcagccagtg ccatgatttc gcgcactgct
      961 ttaccaaatt cacggctttc ccacgcttca ccaatcactt cagcggcatc ggtaaaggtt
     1021 ttgtacagct cagggtcagc cagctcgctt gccagcacac cgtcaaaacg cttgttgata
     1081 aagcccgcat tacgggaggc caggttaacc actttgttca cgatatcggc attcacacgc
     1141 tgaacgaaat cttccaggtt gagatcgata tcatcaatgc gcgaagagag tttcgcagtg
     1201 tagtagtaac gcaggctgtc agcgtcaaaa tgattcagcc aggtgctggc tttaataaag
     1261 gtgccgcgag acttggacat cttcgcgccg ttcaccgtca catagccatg aacaaacagg
     1321 ttggtcggct tgcggaagtt gctgccttcc agcatggcag gccagaacag gctgtggaag
     1381 taaacgatat ctttaccgat gaagtggtac agctcggcgg tggagtcttt cttccagtat
     1441 tcatcgaagc ttacgctgtc gccgcgcttg tcgcacagat tcttgaaaga acccatgtag
     1501 ccaatcggtg cgtccagcca gacgtagaaa tatttgcccg gtgcgttcgg aatttcaaaa
     1561 ccaaagtatg gcgcgtcgcg ggagatatcc cactgttgca ggccggattc aaaccactcc
     1621 tgcattttgt ttgccacctg ctcctgcaac gcaccgctgc gggtccatgc ctgcagcatt
     1681 tcgctgaaag agggcagatc aaagaagaag tgttcagaat cacgcatcac tggtgtagca
     1741 ccagaaacca ccgatttcgg ctcgatcaat tcagtcgggc tgtaggtcgc gccgcagact
     1801 tcgcagttat cgccgtattg atccggggat ttacatttcg ggcaggtgcc tttcacaaaa
     1861 cggtccggca ggaacatgcc tttttccgga tcgtacagct gagagatggt gcggttttta
     1921 ataaaaccgt tttctttcag gcgagagtag ataagttctg acaactggcg gttctcttcg
     1981 ctgtgcgtcg agtgatagtt gtcatagctg atgttaaagc ctgcgaaatc agtctgatgc
     2041 tcctgactca tttcgccaat catctgctcc ggggtgatac cgagctgctg agctttcagc
     2101 atgatcggtg taccgtgggc atcgtcggcg cagatgaagt tgacctcgtg gccgcgcatt
     2161 cgctggtaac ggacccagac atcagcctgg atgtgctcca gcatatggcc gaggtggatt
     2221 gagccgttag cgtacggcag tgcgcacgtc accagaattt tcttcgcgac ttgagtcata
     2281 gtaggcatta cttcttaata gtgaaaaggg gttagatagt accaaatggg aaaatgttaa
     2341 gtaagtactt ctctatattc ttcaggtaaa ctatcagctg atggtttatc tatacaaaaa
     2401 aggagtcggg atgaacgaac aatcccaggc caaatcacca gaagccttgc gcgcaatggt
     2461 cgccgggaca ctggccaatt ttcagcaccc aaccctgaag cataacctca ccacgctgaa
     2521 agcgttgcac catgtcgcct ggatggacga cacgttgcat gtggaactgg ttatgccgtt
     2581 tgtctggcat agcgcgttcg aagagttaaa agagcaatgt agcgccgaac tgctgcgcat
     2641 caccggggcg aaagctatcg actggaaact gtcgcataac atagccactc tgaaacgcgt
     2701 gaagaatcag ccgggaatta acggtgtgaa gaacattatc gcggtgagtt ccggtaaagg
     2761 cggcgtgggt aaatcgtcca cggcggtaaa cctggcgctg gcactggcgg cggaaggggc
     2821 gaaagtcggc attctggacg ccgatatcta cggcccatca attcccacca tgctgggcgc
     2881 agaaaatcag cgtccaactt cgccggacgg tacccacatg gcaccgatta tgtctcatgg
     2941 cctggcaacc aattcaattg gttatctggt cactgacgac aatgcgatgg tgtggcgtgg
     3001 accgatggcg ggcaaggcgt taatgcagat gttgcaggaa accttgtggc cggatctcga
     3061 ctatctggtg cttgatatgc cgccaggcac cggtgacatt caattgacgc tggcgcaaaa
     3121 cattccagta actggtgcag tcgtggtcac caccccgcag gacatcgcgt tgatcgatgc
     3181 gaaaaaaggc attgtgatgt tcgagaaagt cgaggttccg gtgctcggta ttgtcgagaa
     3241 catgagtgtg catatttgca gtaactgtgg tcaccatgaa ccgattttcg gtaccggtgg
     3301 tgcagagaaa ctggctgaaa aataccatac acagctgctg gggcagatgc cgttacacat
     3361 ctccttacgt gaggatctcg ataaaggcac gccaacggtt atcagccgtc cggagagcga
     3421 atttaccgct atctaccgcc agctggctga ccgcgttgca gctcagctct actggcaggg
     3481 tgaagtcatt cctggcgaga tttccttccg cgcggtctaa cgcctattaa ccattctggt
     3541 tattcttcat acaccgcaga gatgttatat tgatgcggtg tatttgtttt tgccgcatta
     3601 atgaacactt atttaatact gcgaatattc tgcggacctt cgtccgaaac tctacaaagt
     3661 cgagtgatcg tacaggcaat gcttcattat catccttcgt gctgtgtagt ggatgtacag
     3721 ctatagtttg ttatgcactc attctatcca ggcataacct ttactacagg gataacgttc
     3781 aatgaataag tattggctgt caggaattat ttttcttgcc tatggattgg cttctcccgc
     3841 agtctcttca gagactgcca cattaacgat taatggcagg atcagtcccc caacatgcag
     3901 tatggctatg gttaatagtc aaccacaaca gcattgtggt cagttaacgt ataatgttga
     3961 cacccgtcat caggtttctt cgcccgtcaa aggtgttacg acagaagtgg ttgttgcagg
     4021 tagcgatagc aaacgtcgaa tcgtgttaaa tcgctacgat taatttggca acctgatttt
     4081 aattgcattt agaaagacga a
//
LOCUS       DAJTOW010000191         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTOW010000191 DAJTOW010000000
VERSION     DAJTOW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.193"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(886..1077)
                     /locus_tag="OO244_003948"
     CDS             complement(886..1077)
                     /locus_tag="OO244_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5665585.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO244_003949"
     CDS             1142..1465
                     /locus_tag="OO244_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5665586.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO244_003950"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO244_003950"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO244_003951"
     CDS             2143..2673
                     /locus_tag="OO244_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5665587.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEVMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO244_003952"
     CDS             2680..2913
                     /locus_tag="OO244_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5665588.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO244_003953"
     CDS             complement(3459..3851)
                     /locus_tag="OO244_003953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665589.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          968 a          915 c         1205 g          954 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac caccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacagac gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacac
     2341 cgggaagggg tgaaagaggt gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgttgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagattttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTOW010000192         3991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTOW010000192 DAJTOW010000000
VERSION     DAJTOW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.194"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO244_003954"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO244_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5665590.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO244_003955"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO244_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5665591.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO244_003956"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO244_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5665592.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
BASE COUNT          913 a         1115 c          962 g         1001 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg g
//
LOCUS       DAJTOW010000193         3900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTOW010000193 DAJTOW010000000
VERSION     DAJTOW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.195"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..66)
                     /locus_tag="OO244_003957"
     CDS             complement(<1..66)
                     /locus_tag="OO244_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5665593.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMW"
     gene            complement(66..2143)
                     /gene="flhA"
                     /locus_tag="OO244_003958"
                     /pseudo
     CDS             complement(66..2143)
                     /gene="flhA"
                     /locus_tag="OO244_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2136..3283)
                     /gene="flhB"
                     /locus_tag="OO244_003959"
                     /pseudo
     CDS             complement(2136..3283)
                     /gene="flhB"
                     /locus_tag="OO244_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3519..>3900
                     /locus_tag="OO244_003960"
     CDS             3519..>3900
                     /locus_tag="OO244_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5665594.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT          969 a         1123 c          956 g          852 t
ORIGIN      
        1 ccacatcccc tccccggcgg cttgcaccag cagcggaaac aataatatcg ctaataaggt
       61 tctcatcatt taccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc
      121 agattcgaca ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt
      181 cgcagcgcgt ggttcaccaa caatactggc ggcgcaccca gcatctcctg acgggataga
      241 gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc
      301 tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca
      361 tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg
      421 cttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc gagaatggtg
      481 cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt atgcagtgtg
      541 gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg
      601 cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg
      661 aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc
      721 tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg
      781 acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc cagccagcgc
      841 cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca
      901 ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg
      961 gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg
     1021 accatcggga tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg
     1081 ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcggca
     1141 ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc ggcagtgaac
     1201 agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag cacggcggcg
     1261 cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc
     1321 tgatcggtgc tgacacgcgt aacgatgccc cccgcggcgg tagaaatcac cagcgccgga
     1381 atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc cgcgtgtccc
     1441 atgctcatgc cagttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga
     1501 tgaggatccc ggcaatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt
     1561 aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca
     1621 atccagcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa
     1681 agcgcgcacc cacttctgct atacgcccgg cacctttggt aatgaccata aagttgatga
     1741 tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac
     1801 cgaacgcttc gaccaccttc ccagccgccg ccgcgccggt atgcccttcc atcaaaatga
     1861 tacgggttga agccacgtta agtgccagac gcaacaacgt agtaaacaac agaatggtcg
     1921 gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg
     1981 acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca
     2041 tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat tatgtcgatt
     2101 tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta
     2161 ataaaatcca gggctgccgg caccggaaga tgagtaggtg tacagggcgc tgtccaccag
     2221 ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca
     2281 gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg
     2341 gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca
     2401 gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg
     2461 ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca
     2521 tccgacgccg tgcggcagct cgctgcatct gacagatccg tcctttaaca tgggggtcgc
     2581 cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt
     2641 ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca
     2701 gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc ggtgactcgg
     2761 ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
     2821 ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc gagaatatcc
     2881 gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa
     2941 ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg ccgctaatca
     3001 gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat
     3061 tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
     3121 gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca
     3181 gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt
     3241 gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa
     3301 gagtattaag cgtaaatgat gccagagcgc aaagcgttca atggtttaag taaggggcaa
     3361 aacaggcggg atttagggcg tttatgcaaa aataggttca gttgcagaac gttttgcgaa
     3421 ccattttcca gttctgatat ttagccattt agcctataga atcgcactcc gcctctctgt
     3481 tagtttgcta ataattaaat tattacaaag gtgatcaaat ggaatggcaa caattgctta
     3541 actgcaaccg tcggaaagat aatctaaaaa aaaccaacat agcattttta catccccttg
     3601 ctagcggaag acaagagatc gagcgggatt acgaccgatt actttttgct gcaccaactc
     3661 gtcggcttgc ggataaaaca caagttttcc cgttagatca aaatgacagc atacggacga
     3721 ggctgactca ttctcacgaa gtagcaaact ttgctcgcgg gataggaatg aggctcgctt
     3781 tcgagatgaa ggagcacatc tttggcgaaa tacccacggg gattgtcgtt gaacgcgacg
     3841 ttcctgctct gttagcagcg atcggactgg cacacgatct cggtaatcca ccatttgggc
//
LOCUS       DAJTOW010000194         3505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTOW010000194 DAJTOW010000000
VERSION     DAJTOW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.196"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            8..886
                     /locus_tag="OO244_003961"
     CDS             8..886
                     /locus_tag="OO244_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5665595.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPSAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            883..2274
                     /locus_tag="OO244_003962"
     CDS             883..2274
                     /locus_tag="OO244_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5665596.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2271..2528
                     /locus_tag="OO244_003963"
     CDS             2271..2528
                     /locus_tag="OO244_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665597.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2623..2823
                     /locus_tag="OO244_003964"
     CDS             2623..2823
                     /locus_tag="OO244_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5665598.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2816..3196
                     /locus_tag="OO244_003965"
     CDS             2816..3196
                     /locus_tag="OO244_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5665599.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     KGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3396..3472
                     /locus_tag="OO244_003966"
     tRNA            3396..3472
                     /locus_tag="OO244_003966"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3430..3432,aa:Ile,seq:cat)
BASE COUNT          927 a          769 c         1006 g          803 t
ORIGIN      
        1 gacagcgatg atgacgttta acctgcgtga acaacaaaaa agactacagg cgcgaatgga
       61 tgagttacgg gcagagattg catttgctca gaagggcgaa aagccatggc cttatcgttc
      121 ctgcctgatg cgtgaaggtc gcggatattg cgaaaaacat ggcgaatatc acacgcatat
      181 actggtgtgg agcgatcgta atggcgagga cagagaagaa atttcatgct gccctgactg
      241 cttaatcgct gaggccaacg atttgaccat ggagctgtcg tccatcaagg cggaagagct
      301 gactgataac gccggaattg ccctgcgttt tcgggactgc gagtttgata attatctgga
      361 ggttaatcct agcgcagcca gaaatcttgc ggcctgtcgc cgctatgcgg agaactggcc
      421 agatatgctg gagaacggta ccagtcttgt tatgaccggc agttgtggta ccggaaaaaa
      481 tcatctggct gtggctatgg caaaacacat catccgtaac tatctggcca gtgtggagat
      541 caccgacgtg atgcgcctta cccgtgctgt gaaaaattgc tggcggaatg acagcgaaaa
      601 aacagcggat gaagttattg agcgttatgc gtcaatggat ttgctgatca tcgacgaagt
      661 tggcgttcag tttggcagcg cggctgaaat ggctattttg caggaaatta tcaacgccag
      721 gtacgaaagt atcttgccca ccattctgat cagtaacctc tcaccggaag agttgtgggc
      781 gttcatcagt ccccgaattg ccgacaggat cacagacggg ggacgcaact ggttgtcgtt
      841 taactggccc agttaccgtt ctcgtatcag aggtgtggct gcatgacaac tccagtatgg
      901 cgtaacgatg accttgaagg cgctgtcatc ggcgcgttct ttctgcgcgg agcagatcct
      961 gaagtgatgg atattctggc cacactgcca gcggacgttt tttctgtacg agcgtatcag
     1021 gatatctata caggcatctg cagacaggcc cgtgtttcag gagtgattga ccccgtgctg
     1081 ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg taaaacctgg
     1141 gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc actgcgcgat
     1201 gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac ctgtgaagca
     1261 gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac aggaaagggc
     1321 gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg tgttgaatgc
     1381 cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga actggacgca
     1441 aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc ttcgatgggg
     1501 aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca tggtgtgctt
     1561 ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc tgcggctgga
     1621 gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga ctggacgcgt
     1681 ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga ccaggcgaac
     1741 ctggccattg atgagatatg cgcaacaacg aaacaccacc tgattaaata tccggaaacg
     1801 gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg gcgtcatgac
     1861 cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg tggttttccg
     1921 ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa acgtcccatg
     1981 aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat tctgatgctt
     2041 tacagggatg aagtgtacaa cccggatacg caggccaggg gcatcgcaga aatcaatatc
     2101 acgaaacaac gtaacggttc tctgggaacg atttaccggc gtttttataa cggacatttt
     2161 ctgcctgtgg accaggaaag tgcacgggtg ctttccactc ccatgaagcc cggcaatccg
     2221 cgcagataca gtaataaacg aactgacagc agtaagatgg aacgtttctt ttgaacaacc
     2281 agacaatgac ttttacccct gaacaattgc gtaaacacgc gcaggaaatg ctgcgtcatg
     2341 ctgaacaact cgaaaaaacg ggcataacaa aagatgcaat ccgtaaagat atggtacccg
     2401 cgcttcggga actgatgcag gcgaagcatc gcgcacagaa agcagtagat gagctggtgg
     2461 actgtgtggc agagctggaa accagagtcg gaaagtttga aaaactggtg caggaggcgc
     2521 tgcgctgatg cgccatgagt ttgttttgcc ctggcttgtg cgcgatcatt atgtccctgg
     2581 tggaaagccc gggataaaaa tcaccgaacc ggagagtatt tgatgagcgt caaaattcaa
     2641 acgataccgg aactgcttat ccagacacga ggtaatatga ccgaagtttc acgaatgctg
     2701 aactgtaatc gcgccacggt aagaaaatat gctgaagata aagagggcaa agggcacgcc
     2761 attgttgatg gtgttctgat tgttcatcgt ggatgggaca ggggtaaaga cagtgatgcg
     2821 tgatattcag caggttcttg agcgctgggg cgcgtgggtg gctaataatc atgaggatgt
     2881 gacctggtcg tccattgctg ccggttttaa gggattaatc ccttcaaaag taaaatcccg
     2941 cccgcaatgc tgtgacgatg acgcgatggt tatatgtggg tgtatggccc gccttaaccg
     3001 gaacaacaac gatctacatg acttgctggt tgactactac gtgtttggga tgacattcat
     3061 gacgctggcg cggaagcatg gatgctcaga tggttatata ggaaagaagc tacaaaaagc
     3121 caagggggtg gtggaaggaa tgttgatgat gttgggtgtc cggttagaaa tggacaaata
     3181 tattgaacat ccctgatggt aaggggcgac tttctagaaa atattttacg tacgtaaaaa
     3241 acgggatatt ctgttaagag tggttgcttc gccacacagc ttaaacccgc ctccgggcgg
     3301 gttttttatg cctgaaaaac ggtacaggac gttaaacacg ctggtggtca gatgagtttg
     3361 cagatgtgat gacatatggt tattattctg cctccggccc tttagctcag ttggtcagag
     3421 cgagcgactc ataatcgcca ggtcgctggt tcaagtccag caagggccac caaccaccac
     3481 tagctcatcc ggatagagca tcaac
//
LOCUS       DAJTOW010000195         3450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTOW010000195 DAJTOW010000000
VERSION     DAJTOW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.197"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(214..417)
                     /locus_tag="OO244_003967"
     CDS             complement(214..417)
                     /locus_tag="OO244_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665600.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(419..574)
                     /locus_tag="OO244_003968"
     CDS             complement(419..574)
                     /locus_tag="OO244_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5665601.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(777..1184)
                     /locus_tag="OO244_003969"
     CDS             complement(777..1184)
                     /locus_tag="OO244_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5665602.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1261..1488
                     /locus_tag="OO244_003970"
     CDS             1261..1488
                     /locus_tag="OO244_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5665603.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1472..1894
                     /locus_tag="OO244_003971"
     CDS             1472..1894
                     /locus_tag="OO244_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665604.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1972..2755
                     /locus_tag="OO244_003972"
                     /pseudo
     CDS             1972..2755
                     /locus_tag="OO244_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2762..3450
                     /locus_tag="OO244_003973"
                     /pseudo
     CDS             2762..3450
                     /locus_tag="OO244_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          760 c          940 g          865 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgaactat ccgggtatcg cgcgtctgtt
      181 tttgtttgcg aattcagtca atgcgcatgg tatttatacc ccgccaagac ccggtgaata
      241 tcctgcacgg ccttctgctt tgtagaaggc ttcttcgcta tcataatcgc cagattccag
      301 cgcttcttta gccagccaga tgcgtgcccc agtgcctgca tttggctcca gttgctggag
      361 gcgttttgca tcttctagga gtagagcgat aacgtgtttt aattctgtct cgttcatttt
      421 actcacctga atgtcttccc aaccaacgac gtgcgccagc ttcggtttta aacgttttgc
      481 ttttggtata cgttatggcg gtgaatgtgc cgtcctggtt ggggaacacg ccgtacaccg
      541 gagattcgtt gttgccaaga tcgatagtat tcatgttgac cccatttccc cttaacgccg
      601 gggtagcgga actgtttgct gagaacaccg tgcggtgtct tgatgaataa aatttagaat
      661 aacctaagga gggtgatcaa gatttttgtg tagaaaaacc taagtttttt gatgtaaaaa
      721 acacaagtgt ttgaaagttt gtgcttttta ttacagggtg tggagaaaaa aggggattat
      781 ttgtttgcgc ttcttttgcg agctttgagt agttcttcaa aaagtttgtt gaaattttca
      841 actcgagcac gcatctctga caacagggcc ttttgctctg actcaggcag tgcgtcgaac
      901 agttgaagta actctttttg atcttctgtc agaatggctg gctgattatc cgggatcggt
      961 tcgcctggtt gtttatcttc atccccaaaa agaagccaag tcggtgagca ctgaagcgct
     1021 tggctcagtg cgaataatct tttccccgcc ggctgtgttt catctctttc ccattgagaa
     1081 attgttacgt gagccacttt gaccagctta cctaatgcgg cctgagacag ttttaatttt
     1141 ttacgcctat gtaagaggcg agcaccgaag gtttcgtttt tcatattagg gaattctaat
     1201 ttttcttgac ttaggtttct ctacggcctg gtttccttag gaaaatctaa ggagctcgat
     1261 atgttgaaaa ttgatgctat agcgtttttt ggcagcaaaa caaagcttgc caatgccgca
     1321 ggagttaggc tggcaagcat tgctgcatgg ggggaactgg ttcctgaagg tcgcgcgatg
     1381 cgcctgcaag aggcatctgg cggggaactt cagtacgacc ccaaagttta tgacgaatat
     1441 cgtaaggcaa aacgacctgg gaaggtgatt catgaaaatc aggcatgagc acatcgaatc
     1501 agtgctgtta gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg caattactga
     1561 agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg actggaacaa
     1621 tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa gggaaaaaat
     1681 tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc accgactctg
     1741 tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt tgagtacggc
     1801 aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt tcagcggtgg
     1861 tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc cggattgttt
     1921 tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca aatggcttca
     1981 aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag gcttgctgag
     2041 attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg ggcatgggcg
     2101 gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac aaaaagtgca
     2161 atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt tggatggctg
     2221 gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg aaaaagctct
     2281 aaaaaacggg cggttacaaa cgagcgagta acaaaaatac gcgaactgaa acgaaaaggt
     2341 aacgctgcca gcgttacaca aacggatcaa aaagcgttac cagtggaaga ggaagaggaa
     2401 gaggaagatc taaatactga tctcccccta aatccccctc gccaaaaacg agcgtctaaa
     2461 aaattcgagc cggaggctat tgagctgccc gattggttgc cggaaacact ctggcatgag
     2521 tgggtccggt tcagacaggc attgcgaaaa ccgattcgaa cggagcaggg cgatacggga
     2581 actggaaaaa ttccgtcagc agggttttac acctgagcag gtgattcgac acagcatcgc
     2641 caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca
     2701 ggttaacacc gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacggac
     2761 catgaaaaat attgcgacag gcggcgttct ggaacgcatc cgccgactga ccccgcaaca
     2821 tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag tggcaacttg ctgaaggcca
     2881 gaaacgtagc gaggagatca accgcctgaa tcgccagttg cgggtggaaa aaattctgaa
     2941 tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt gcgaattacc aggtgcagaa
     3001 cgacggccag cgatacgcgt taagccaggc gaaatccatc gccgatgaac tgatgaccgg
     3061 gtgtacaaat tttgcgttca gcggaaaacc tgataccggg tagaatcact tagcggcagc
     3121 tatcgggaat cgcctgctga aagacggtca gacagtgatt gtggttaccg tggctgatgt
     3181 tatgagcgcc ctgcacgcca gctatgacga cgggcagtca ggcgaaaaat ttttgcggga
     3241 gctgtgcgaa gtggatctgc tggttcttga tgaaattggc attcagcgcg agacgaaaaa
     3301 cgagcaggtg gtgctgcacc agattgttga tcgccggaca gcgtcgatgc gcagcgtggg
     3361 gatgctgaca aacctgaact atgaggtgat gaaaacattg ctcggcgagc gggtgatgga
     3421 tcgcatggtc atgaacggcg ggcgctgggt
//
LOCUS       DAJTOW010000196         3436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTOW010000196 DAJTOW010000000
VERSION     DAJTOW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.198"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            51..473
                     /locus_tag="OO244_003974"
     CDS             51..473
                     /locus_tag="OO244_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5665605.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            531..887
                     /locus_tag="OO244_003975"
     CDS             531..887
                     /locus_tag="OO244_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665606.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            980..1198
                     /locus_tag="OO244_003976"
     CDS             980..1198
                     /locus_tag="OO244_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5665607.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            1200..1565
                     /locus_tag="OO244_003977"
     CDS             1200..1565
                     /locus_tag="OO244_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5665608.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            1562..2227
                     /locus_tag="OO244_003978"
     CDS             1562..2227
                     /locus_tag="OO244_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665609.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            2227..2592
                     /locus_tag="OO244_003979"
     CDS             2227..2592
                     /locus_tag="OO244_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5665610.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPLGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            3047..3202
                     /gene="hokD"
                     /locus_tag="OO244_003980"
     CDS             3047..3202
                     /gene="hokD"
                     /locus_tag="OO244_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5665611.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          971 a          715 c          905 g          845 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg accttatctg gtcattacct gacggagaaa
      361 tacgccgcta cgacaggcgc ctgaatataa tctgtcgcga gtgccggaag agcgaagcta
      421 tgcagcgtgt actggctttc tatcagggta attttcagga ggcgatactg tgaatgaaat
      481 tagctatcag gcttcaatta ccgctggcat tcgcatcaaa ggagaggagc atggaaataa
      541 aaccagaaga tgagttaagc aatatcgttt tatttccgat aaaagaggat gaccctcgta
      601 atcaggttaa ttttctttat aagccatcgg aaagaccata ttgccatcac gcctctgtac
      661 gggttgacga aaaagagcgt caggtccgct gtaaaatctg cggtgcggtt gtggagccgt
      721 ttgactggat gctctctgtg gcaaaaagag aaaccagact ggcagatgat gtaaggctat
      781 tgcgccagga ggaacaggaa aggcggagaa atatagaaaa gctgatacag attgagcgta
      841 acgcgaaagc gcggatacgc agggcgacaa aatccagaac tgaataatta aatttagcac
      901 tgttaaaaat ttaatcctta accggaggga tttctgcacc ctcaaatcat caggagaccg
      961 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
     1021 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgca
     1081 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
     1141 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
     1201 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
     1261 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
     1321 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagaatgcc
     1381 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
     1441 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
     1501 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
     1561 catgactact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
     1621 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
     1681 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
     1741 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
     1801 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
     1861 agttgaccag gaatctaata cgttcactgc tatatatttt tacaaagcgg aagttgagcg
     1921 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1981 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     2041 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     2101 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     2161 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     2221 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     2281 tgtattgggc aactttgtaa aattccacta ggagatatgg ttgaaatcgt ggaaatggcc
     2341 atgcgtaagg ctggcaactc tccggtaact ctggctggct ggataagctg tagtgatgca
     2401 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     2461 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     2521 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     2581 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     2641 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     2701 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     2761 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaat tagcctggat
     2821 agaaggtgct ggcaataaaa aatagcgttt tcttatcggt atcggtaaga ttgctgcggg
     2881 tgcttgaggc tgtctgcctc gggcatgcca ctgtaaggca gacagagaaa agccccagtt
     2941 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     3001 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     3061 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     3121 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac
     3181 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctcat
     3241 gtgtcaggca tcctcaacgc acccgcacta aacccgcttc ggcgggtttt ttgttgcgtg
     3301 ctgaatacgc agggtgaaaa ataaccatat atttgattat atacacaaca aaaaataaaa
     3361 gtcattgtac ttgcacatta aacaatcaaa tatacggcgt gaaataaata tttatcagat
     3421 taatattttt gtctct
//
LOCUS       DAJTOW010000197         3428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTOW010000197 DAJTOW010000000
VERSION     DAJTOW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.199"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..331
                     /locus_tag="OO244_003981"
     CDS             <1..331
                     /locus_tag="OO244_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCR5665612.1"
                     /translation="YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNI
                     AGVEEKSTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKA
                     AGFKEVK"
     gene            complement(372..842)
                     /locus_tag="OO244_003982"
     CDS             complement(372..842)
                     /locus_tag="OO244_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5665613.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(889..1608)
                     /gene="yehT"
                     /locus_tag="OO244_003983"
     CDS             complement(889..1608)
                     /gene="yehT"
                     /locus_tag="OO244_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5665614.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1605..3290)
                     /gene="btsS"
                     /locus_tag="OO244_003984"
     CDS             complement(1605..3290)
                     /gene="btsS"
                     /locus_tag="OO244_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5665615.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          910 a          895 c          782 g          841 t
ORIGIN      
        1 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
       61 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
      121 atacaaaaac atcgcgggtg ttgaagaaaa atcaacctat accgatacct acgcgcagga
      181 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
      241 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
      301 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggcca
      361 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
      421 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
      481 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatagccag aatgtcatcg
      541 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
      601 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
      661 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
      721 agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag aatacgcacc
      781 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttatc
      841 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
      901 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
      961 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     1021 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac gtaatgtcaa
     1081 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     1141 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     1201 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     1261 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     1321 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     1381 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     1441 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     1501 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atatacgcag
     1561 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     1621 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     1681 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     1741 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     1801 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     1861 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     1921 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     1981 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     2041 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     2101 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     2161 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgccct
     2221 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     2281 ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     2341 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     2401 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     2461 taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     2521 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagtacc
     2581 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     2641 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     2701 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     2761 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgcaaggatg
     2821 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     2881 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     2941 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     3001 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     3061 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     3121 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     3181 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     3241 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     3301 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     3361 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     3421 ccctatag
//
LOCUS       DAJTOW010000198         3364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTOW010000198 DAJTOW010000000
VERSION     DAJTOW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.200"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            2..394
                     /locus_tag="OO244_003985"
     CDS             2..394
                     /locus_tag="OO244_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR5665616.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            532..1416
                     /locus_tag="OO244_003986"
     CDS             532..1416
                     /locus_tag="OO244_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCR5665617.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="OO244_003987"
     CDS             complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="OO244_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR5665618.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2753..>3364
                     /gene="tetR(A)"
                     /locus_tag="OO244_003988"
     CDS             2753..>3364
                     /gene="tetR(A)"
                     /locus_tag="OO244_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR5665619.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLV"
BASE COUNT          638 a         1021 c         1057 g          648 t
ORIGIN      
        1 cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc cggcgacgaa
       61 tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc ccgtcgaagg
      121 gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg tcttttatgg
      181 cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca tggccgggat
      241 aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg actacgacgt
      301 gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag ctctgttgcg
      361 ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat gatcgcggcc
      421 atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc ggtcatcgcg
      481 gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg catgtcatta
      541 cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag cacctacatt
      601 gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct gcgggcgttg
      661 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
      721 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
      781 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
      841 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
      901 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
      961 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     1021 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     1081 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     1141 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     1201 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     1261 ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag tgcgcttcaa
     1321 atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc caaccgcact
     1381 cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag gcctatcgtt
     1441 tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga cgcagcgccg
     1501 gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta
     1561 tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga
     1621 gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca
     1681 ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg
     1741 ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc
     1801 cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct
     1861 gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc
     1921 agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt
     1981 gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga
     2041 acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact
     2101 ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga
     2161 acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc
     2221 cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga
     2281 tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc
     2341 gcccgatata gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag tcgacagcag
     2401 cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca
     2461 gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg
     2521 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
     2581 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg
     2641 gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta
     2701 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
     2761 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
     2821 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
     2881 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
     2941 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
     3001 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
     3061 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
     3121 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
     3181 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
     3241 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccggtcc
     3301 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     3361 cgtt
//
LOCUS       DAJTOW010000199         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTOW010000199 DAJTOW010000000
VERSION     DAJTOW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.201"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            102..3005
                     /locus_tag="OO244_003989"
     rRNA            102..3005
                     /locus_tag="OO244_003989"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO244_003990"
     rRNA            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO244_003990"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          702 c          992 g          656 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc catgcgagag t
//
LOCUS       DAJTOW010000200         3188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTOW010000200 DAJTOW010000000
VERSION     DAJTOW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.202"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            32..670
                     /locus_tag="OO244_003991"
     CDS             32..670
                     /locus_tag="OO244_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5665620.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPCVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            673..1836
                     /locus_tag="OO244_003992"
     CDS             673..1836
                     /locus_tag="OO244_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5665621.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1920..>3188
                     /locus_tag="OO244_003993"
     CDS             1920..>3188
                     /locus_tag="OO244_003993"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5665622.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSI"
BASE COUNT          708 a          759 c          928 g          793 t
ORIGIN      
        1 cgcagcttgt atgacatggt aatggacgat catggccaga aaacgcaaat caagaaataa
       61 cagtaaaatt ggtcacggag cgatcagtcg cattggtaga ccgaataatc cttttgaacc
      121 gcgtcgcaat cgctacgcac aaaaatactt aacgttggcg ctaatgggcg gtgccgcttt
      181 tttcgtattg aaaggttgta gcgatagtag cgatgtcgat aacgacggcg acggaacatt
      241 ttacgcgaca gtgcaggatt gtattgatga cggtaataat gccgacattt gcgcgcgtgg
      301 ctggaacaac gccaaagcgg cattttatgc cgatgttcca aagaatatga ctcagcagaa
      361 ctgtcagtct aagtacgaaa attgctacta cgacaatgtt gaacagagtt ggatcccggt
      421 cgtttctgga tttttgttaa gccgggttat tcgtaaagat cgcgatgagc cgtgcgtcta
      481 taacagcggc ggatcctcat ttgcttcccg ccccgtctgg cgcaacactt ccggtgatta
      541 ctcctggcgc ttcggttctg gcaaaaaaga gtcttactct tcaggcggct ttaccaccag
      601 aaaagcgtcg accgtttcgc gcggcggcta tggtcgttct tccagcgccc gtgggcattg
      661 gggaggctaa tcatgctgag acacaacgtt cctgtgcggc gggatctgga ccagatcgcc
      721 gctaacaacg gtttcgactt tcatatcatc gacaatgaaa tctattggga cgagagtcgg
      781 gcttaccgtt ttactctgcg ccagattgaa gagcagatcg aaaaaccgac tgcggaactg
      841 catcagatgt gccttgaggt ggtggatcgc gcggttaaag acgaagagat cctgacgcaa
      901 ctggcgatcc cgccgttgta ctgggatgtg atcgccgaaa gctggcgcgc acgcgatcct
      961 tcgctgtatg gccggatgga ttttgcctgg tgtggcaatg cgccggtgaa gctgctggag
     1021 tacaacgccg atacgccgac ctcgttgtat gagtcggctt atttccagtg gttgtggctg
     1081 gaggatgccc ggcgcagcgg cattattccg cgtgatgccg atcagtacaa cgccattcag
     1141 gaacgcctga tttcgcgctt tagtgagctt tacagtcggg aactgtttta tttttgctgc
     1201 tgtcaggaca ccgatgaaga caggagtacc gtgctgtact tgcaggactg cgcccagcag
     1261 gcagggcagg agtcgcggtt tatctacatt gaagatctcg gtttgggcgt cggcggcgta
     1321 ctgaccgatc ttgatgataa tgtcatccag cgtgcattta agctgtatcc gctggagtgg
     1381 atgatgcgtg acgataacgg tccgctgctg cgcaagcgtc gcgagcaatg ggtggagccg
     1441 ttatggaaaa gtatcttgag taataaaggg ctaatgccgc tgctttggcg cttcttccct
     1501 ggtcatccta accttcttgc atcctggttt gagggtgaaa aatcgcagat tgccgctggc
     1561 gaaagctatg tgcgtaaacc gatctactcg cgcgaaggcg gaaacgtcac cattttcgac
     1621 ggtcagaata acgtcgttga ccacgctgat ggtgattacg ccgatgaacc gatgatctac
     1681 caggcgtttc aacctctgcc gcgatttggc gatagctaca cactcatcgg tagctggatt
     1741 gtcgatgatg aagcgtgcgg aatggggatc cgtgaagata acacactgat caccaaagac
     1801 acctcacgtt tcgttccgca ttacattgct ggataagaat gttttagcaa tctctttctg
     1861 tcatgaatcc atggcagtga ccatactaat ggtgactgcc attgatggag ggagacacag
     1921 tgcactggca aactcacacc atttttaatc aacctatacc attaaataac agcaacttat
     1981 acctgtctga tggcgcgctt tgcgaagcgg taacgcgtga aggtgctggc tgggatagcg
     2041 attttctagc cagtattggt cagcagttag gaacggctga atcccttgaa ctggggcggc
     2101 tggcgaatgt aaacccgcct gaactgttgc gctacgatgc ccaggggcgg cgtctggacg
     2161 atgttcgttt ccatcccgca tggcacctgc tgatgcaggc gttatgtacc aatcgggtgc
     2221 acaatcttgc ctgggaagaa gacgctcgct ccggcgcatt tgtggcgcgc gcggcgcgtt
     2281 ttatgttaca tgcgcaggtt gaggctgggt cgttatgtcc aataacgatg acctttgccg
     2341 ccacgccatt gttgttacag atgttaccca cgccgtttca ggactggacc acgccgctgc
     2401 tgagcgatcg ctacgattct cacttattgc caggtgggca aaaacgcggt ttgttgattg
     2461 gcatgggaat gacggaaaag cagggcggtt ccgatgtcat gagcaacacc acccgtgcag
     2521 agcgtttgga agatggctct tatcggctgg tggggcataa atggtttttc tcggtgccgc
     2581 aaagcgatgc gcatctggtg ctggcgcaga ccgcgggcgg tctgtcctgc ttttttgtgc
     2641 cgcgcttttt gcctgacggg caacgcaacg cgattcgcct cgagcggctg aaagataagc
     2701 tgggtaatcg ctctaacgcc agttgcgaag tggagtttca ggatgccatt ggctggttat
     2761 tggggcagga aggggaagga attcgtctga tcctgaaaat gggggggatg acgcgttttg
     2821 attgcgccct gggtagccat gccatgatgc gccgtgcatt ttcgctggcg atttatcatg
     2881 cacatcaacg ccatgttttt ggtaatccat tgatccaaca gccccttatg cgtcatgtct
     2941 taagtcgcat ggcacttcag cttgaagggc aaacggcgtt gctgtttcgt cttgcgcgag
     3001 cgtgggaccg gcgtgccgat gccaaagaag ccctgtgggc gcgtttattt acgcctgcgg
     3061 caaaatttgt gatctgcaaa cgaggtatgc cgtttgtggc cgaagcgatg gaggtgctgg
     3121 gcggcattgg ttattgcgag gagagcgagc tgccgcggct ttaccgggag atgccggtaa
     3181 acagtatt
//
LOCUS       DAJTOW010000201         3183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTOW010000201 DAJTOW010000000
VERSION     DAJTOW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.203"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..543)
                     /gene="fruK"
                     /locus_tag="OO244_003994"
     CDS             complement(<1..543)
                     /gene="fruK"
                     /locus_tag="OO244_003994"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="converts fructose-1-phosphate and ATP to
                     fructose-1,6-bisphosphate and ADP; highly specific for
                     fructose-1-phopshate; similar to PfkB; forms homodimers;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5665623.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAP"
     gene            complement(543..1673)
                     /gene="fruB"
                     /locus_tag="OO244_003995"
     CDS             complement(543..1673)
                     /gene="fruB"
                     /locus_tag="OO244_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5665624.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2041..>3183
                     /gene="setB"
                     /locus_tag="OO244_003996"
     CDS             2041..>3183
                     /gene="setB"
                     /locus_tag="OO244_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5665625.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRCDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMII"
BASE COUNT          660 a          867 c          812 g          844 t
ORIGIN      
        1 cggtgccgct ttcaaacctg ctactaacgc ttcacggcta ctatcaaaga taatgcaagg
       61 acactgacta cgcaggcgag tcatccagtc ggtgaacgct tccgggctga cgcctgacgg
      121 taagcttccg ctgacacaga ccatatcgaa ctgaccgagc cagctcagag aatcagtcac
      181 aaagcgttcc cagtcggcgg gggtgacttc gaaacccgag aagttgaagt cggtcacttc
      241 gccgtctttt tccgtcagct taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg
      301 gttggcaatg cccagctcgc tgaacagttg ctgaaaacca tcctgattgt ctttacccag
      361 gaagccgcca acggtgacat caattcccag gtcttttaat actttggcca cgttgatgcc
      421 tttacccgcc gcatgcagac cggtggtttt caccaggttc acttcgccgc gttcaatttc
      481 cgggcagaaa ccaacaaggt cataagccgg attaagggtg atcgtagcaa cacgtctgct
      541 cattatgcgc cctccccaag accagcagcg atagcgtcgc caatggcttt cagcgcctgt
      601 tcagcatctg caccctgggc ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc
      661 acaactttca tcagactacg tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg
      721 gtaatatcac tgttaaattg tttaatggta ttgaccagca tggtacctgg acgagcatgc
      781 aggccgtgtt cattgcgcac cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca
      841 tcgctggtca gcagcgccag caacgttgcc gcatccgctt tcagcaagcg gtcagcttta
      901 ttgtcgagca acaaatcagc gagacgctta agaaccgcga tgggctgatc gtcattcatc
      961 gccacactca ccagcatggc tgccgtttcg ccgtccacat caaaagcatt tgccgcacgg
     1021 cttaccgcaa tcgcactacg cagattgcct tcggcgctat cgctcagcca gataccctgt
     1081 ccgagattca gcggttgttc attgatggct ttggtgacga aagtggcgtc aactgccctc
     1141 gcctctttca gacgcgcagc gttcagcgcc tgaagagtca gcagatcgct ggcgacgata
     1201 tccagcgtca gcatttcgtt gtcgagcttc agctgctcac tctgcttttc gcccatcagt
     1261 aatgcgcgaa gttcttctgc tgttgttgct gacttcagtt gttcagcaac ggaatcatcg
     1321 ctcagtacgt gggtcagctg gcgtagcagg cccagatgtt tatccgagct ggcagcaata
     1381 ccgattgcca cgtacgctac ctgaccgtca ccccaggtga cgccttccgg gaactgaaat
     1441 acctgaacgc cggttttcag cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca
     1501 ataccattgc cgaggaacgt tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag
     1561 ccttctgcta cattaccggc ctgcaccagc gccgcagcga cctggcgaat cgcctcttct
     1621 ttgtctccgg ccttttcgcc cggatggatg tcctgtacgg ataactggaa catagttctc
     1681 ctctcttgct gaattgaaac gattcagcct ctatgagaaa aaaagcgcca acctagctta
     1741 gggttaaaga caagatcgcg ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca
     1801 cgcaaggaaa gccgtaaagt tatgagcaaa agtttgatgt gctgcacaat tttttgtcat
     1861 ttttcctaat tgctgatggg aaaatccgtt atcagcaatt tcatttcagc agcgtcagca
     1921 gccaatcagc aacagtgaaa tggcattttg attattcgtg gcgcttttga tttcgctgtc
     1981 tgtttatttt ctgacaagca gcgtaaactc cgcgtcttcc tcttccagtg atcgaccagc
     2041 atgcataact cccccgcagt ctccagcgcg aaatcgtttg acctgacctc gacggcgttt
     2101 ttaatcgttg cctttctcac cggtattgcg ggtgctctgc aaaccccgac actcagtatt
     2161 ttccttaccg atgaagtaca tgcccgtccg gcgatggtgg gattcttctt taccggcagc
     2221 gctgtcattg ggattctggt aagtcagttt ctcgctgggc gctctgataa gcgctgcgat
     2281 cgcaaatcgc tgattgtctt ttgctgcctg ttaggcgtgc tggcctgcac cctttttgct
     2341 tggaatcgca actactttgt tttgctattc gttggcgtct ttcttagcag ctttggctcg
     2401 accgctaacc cgcaaatgtt tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag
     2461 gcggtgatgt tcagctcttt tttacgcgct caggtttcac tggcatgggt cattggccca
     2521 ccgctggctt atgcgttagc gatgggtttc agctttacgg taatgtatct gagcgcagcg
     2581 gtagcgttta ttgtttgcgg cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag
     2641 cttccgctgg cgaccggcac ggttgaagcg ccgcgccgta accgtcgcga tacgctactg
     2701 ctgtttgtca tttgtacatt gatgtggggc tcgaacagcc tgtacatcat caacatgccg
     2761 ctatttatta tcaacgaact gcatcttccc gagaaactgg ccggtgtgat gatggggacc
     2821 gccgccgggc tggaaatccc gacgatgttg attgccggat atttcgccaa acgtctgggt
     2881 aagcgtttct taatgcgcgt tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg
     2941 atggcgcatt cacctgccat tctgttgggc ttgcagctgc taaatgctat ttttattggc
     3001 attctgggcg gcatcgggat gctctatttt caggatttga tgcccggtca ggcaggttca
     3061 gccaccacgc tctataccaa cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg
     3121 ggcatcgtcg ccgagatctg gaattatcac gctgtgttct ggtttgcgat ggtgatgatt
     3181 atc
//
LOCUS       DAJTOW010000202         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTOW010000202 DAJTOW010000000
VERSION     DAJTOW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.204"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            420..1529
                     /gene="fepE"
                     /locus_tag="OO244_003997"
     CDS             420..1529
                     /gene="fepE"
                     /locus_tag="OO244_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5665626.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2554..2748)
                     /locus_tag="OO244_003998"
                     /pseudo
     CDS             complement(2554..2748)
                     /locus_tag="OO244_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          800 a          658 c          777 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggggg gctgctagcg gcgcggtgtg tttttttaca
       61 ggacgcccct gggggcgctg ctaggggtgt ctgttcagat atgtagcaat gagtcaggga
      121 ttcacgaact gaaaatgtcc gaaaatgtgt ctcatgggca ctgtatgcgc ccaaaccgat
      181 cgtcattcac tttcatgcat agctatgcag tgagctgaaa gcgatcctga cgcatttttc
      241 cggtttaccc cggggaaaac atctcttttt gcggtgtttg cggcagaatc gcgttcagcg
      301 cgttttagcg gtgcgcgcaa taaaatgtta tgttaaatgt ctgctgaatg gtttttaggt
      361 tttttcctaa catatgatag aataaaaaaa ccatatgtta atatattgtg tttttttcaa
      421 tgaaaatctc aaaggtctct gacgaacatt tttctaatta caacatgttg ccgaatggta
      481 atgaagaaat tgaccttttt ggcttgatta gcgttttatg ggcagcaaaa ataaaaattg
      541 ttggggtgat tctatttttt gcttgtattg gctttcttgt ttcttttgtt ttgccacaaa
      601 aatggactag ttctgctgtg gttactcctg ctgagtctgt tcaatggagt gaattaaata
      661 aggagctttc caagcttcat gttctgggag tggattttga tatcaatcgt gatagcgcat
      721 ttgctctgtt tattaagaaa tttcagtccg ttaattcgtt gaatgagtat atgagatcat
      781 ctccttatgt tatggagatt attaaaaaga ataacattag cgctctcgaa ctgcatcgtg
      841 caattgttga gttgagtaaa aatatgaagt cgattgatga taatgccagt aagaaaaacg
      901 acaagtcatc attgtatgta tcatggactc tgagttttac tgctccaaca agtaaagaag
      961 ctcacgatgt gttgtctggg tatattaatt atgtttcttc ccttgttgta agggatttga
     1021 tggaagatat aagaaataaa ctagaagtta aaactaatgt tgaaaaagaa attcttgcac
     1081 tggatgagat aaaaattaga aaccagctga atgcagatat tcgacgcctc aattattcac
     1141 tggaggttgc taatgcggct ggaataaaaa aacctgtata cagcaatggt cagattatga
     1201 aggatgaccc agattttcct gtggctctcg gttctgatgg tatagcaact aaattgaaca
     1261 tcaaaaaatc aatcaaggat gtttcggaat tgagtgggga gttgcgaaat cgtcaatatg
     1321 ttgtgaatca attggttgtg gcgaaagtgg gggatgttga ttttatgcca tttcagtatc
     1381 aattgagccc aacgttaccg gtcagaaagg atgggccaag taatgccata attgtaattc
     1441 tttcttctgt cattggaggg atgcttgcat gtggctatat acttttacgt catgccatgg
     1501 aggccagaaa gatgagtgtg attgagtaaa agttgtgctg atgcatcatt ttataagcaa
     1561 cttttataaa aagttcaaag gatcttcttg agatcattcc cttacagccg tagtcttcta
     1621 ctctagaaaa cgaaaaaacc acctggggag gtggtttttc gaaggttcag taagttgggg
     1681 aacttctgaa ccgtggtaac agtcttgtgc gagacatgtc accaaatctg tccttctagt
     1741 gtagcctcag taaggctgcc acttcaagaa ctctcaatac atctctcgca catcctgttt
     1801 accagtggcc gctgccaatg gcgttaagtc gtgtcttacc gggttggact caagacgata
     1861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1921 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacgaa cgcggccata
     1981 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac
     2041 cagggggaaa cgcctggtat ctttaagtcc tgtcgggttt cgccacctct gacttgagcg
     2101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca ccggcgcggc
     2161 cttttttcgg ggcctctttt ttgctcacat gttttttctg gtgttatccc ctgattctgt
     2221 ggataaccgt atatccgtct ttgcgtgagc agacaccgct cgccgcagcc gaacgaccga
     2281 gcgcagcgag tcagtgagcg aggaagcgga agagcgccgg aagctgcatt ttctccttac
     2341 gtatctgtgc agcatttcac acctcatatg tcgctgttct ctcaccatct gctctgccgc
     2401 ctcataatta agccagtata cactccgtta tcgctacgtg actgggtcat ggctgcgccc
     2461 cgacacccgc taaaaccccc tgacgcgccc tgacgggctt gtctgctgac gaaaacgccc
     2521 acgggaaagc cgtgaccgcc tccgtcagct tcgttatgtt cctgtttcac tttcggcctt
     2581 aaagcgaatc tggatgctgt tctggagttc ttctgcgagt tcgtgcagtc gtttgcacat
     2641 ggccgcctgt tcgtcggcat ccagagcatc cagtttttca agcagtgtca ggctctggct
     2701 tttgatacat cgcgccatat tcagcgcagt ttgttgttat ttgttcacct tccgttgttc
     2761 tccgtcagtt ccgggtgtcg ggggggggcc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAJTOW010000203         3133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTOW010000203 DAJTOW010000000
VERSION     DAJTOW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.205"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            54..1085
                     /gene="ynaI"
                     /locus_tag="OO244_003999"
     CDS             54..1085
                     /gene="ynaI"
                     /locus_tag="OO244_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5665627.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1136..2749)
                     /gene="mppA"
                     /locus_tag="OO244_004000"
     CDS             complement(1136..2749)
                     /gene="mppA"
                     /locus_tag="OO244_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5665628.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          830 a          720 c          688 g          895 t
ORIGIN      
        1 ccgccagggg tttccggcat gacgacaatt atctggagga gcctgataaa acaatgatcg
       61 ctgaactgtt tacaaataat gcgcttaatc tggtcattat tttcggtagc tgcgcagcat
      121 tgattctgat gagcttttgg tttcgccgtg gaaatcgtaa aagaaaagga tttttattcc
      181 atgcggtgca atttttaatc tacaccataa ttatcagtgc tgttggtagc atcagtaatt
      241 atgttataga aaactataaa ctcaaattta tcaccccagg cgttatcgat tttatctgta
      301 cgtccctgat tgcggttatt ctgacgatta agctatttct gctgattaat cagtttgaaa
      361 aacagcagat taaaaaaggt cgcgatatca ccagtgcgcg gattatgtcg cgcattatca
      421 aaatcaccat tattgtagtg cttgttctgc tttatggcga acatttcggc atgagccttt
      481 ctggcttgct gacctttggt ggtattggtg gtctggctgt cggtatggcc ggtaaagata
      541 ttctgagtaa tttcttttcc gggatcatgc tctatttcga ccgtcctttc agtattggcg
      601 actggatccg ttcaccggac agaaatatcg aaggtacagt agcggaaatt ggctggcgaa
      661 ttaccaaaat tacgaccttt gataatcgtc cattgtacgt accgaactcg ctgttttcgt
      721 cgatcagcgt agaaaaccca ggacgaatga ccaaccgccg cattaccacg accattggtt
      781 tacgttatga ggatgcggca aaagtgggcg ttattgtcga agccgtacgt gagatgctga
      841 aaaatcaccc ggccatcgac cagcgacaaa ccttactggt ttatttcaac cagtttgctg
      901 actcgtcatt gaatattatg gtttattgct ttaccaaaac cacggtatgg gctgagtggc
      961 ttgctgcaca gcaagacgtt tatttgaaga ttatcgatat tgtacagtca cacggcgcgg
     1021 actttgcctt ccccagccag acgctgtata tggataacat tacaccgccg gaacaggggc
     1081 gctaaatcag acatcactgg cgttatccac aagggtaacg ccagttccca tcacatcaat
     1141 gcttcacaat atacatagtc cgactgtacg ctacatcttc agggttatta atgggataac
     1201 ctttcagcca cggtttgatt aatcgtccat tggtatattg ataaattggt gcaatcggtg
     1261 cttgctccat gaggattttt tctgccgcgt tgtaatcggc attacgcgct ttaacggtat
     1321 tttccgtcga tgcctgggcc agaactttgt cgtatgccgg attgttaaag cgtgaaatat
     1381 ttcctgaatg cattgacgtt aataatgtca ggaaagtgga gggttcatta taatccccca
     1441 cccatgaggc gcggataaca tcaaaattgc cggtgttacg gctatcgata taggttttcc
     1501 attcctgatt ttgcaattta acatctacgc caaggttctt tttccacatc gatgctacag
     1561 caatcgcaat tttttgatgg ttttctgaag tgttatacaa aagcgtcagc ttcagcggtt
     1621 tttgcggacc ataaccagct gcgctcaaca aagtttttgc ctgcgcattc agttcttcct
     1681 gactcatttg ttcaaacggc gaaggttccg gcgtaaatcc ggcggtaaca tctggtataa
     1741 aatgccacgc tggcttttcg cccgtcccca acactttttc ggtcatcagg cggcgatcta
     1801 tcgtcatact taatgccagg cgaacgcgct gatctgccgt cggccctttt tgcgtgttaa
     1861 aggcataata ataggtcccg agctgcggtg gcgtataaac ctgccccgga atatccttca
     1921 acagcttctg atacatattt ttcgggaagg attcggtgat atcaatatcc cccgcgaggt
     1981 aacgcttagt ggctgcggat tcctgattaa ttggcaggaa ggtcactttt tgcagcaccg
     2041 ttttggcgtt atcccaataa tgggtattcg gtaccacgac cagtttttca ttgactacgc
     2101 gctctttaag aacataagcg ccattgccga tcagatttcc gggtttcgtc cactctttac
     2161 cgctttctac gttggctttt tgcaccggga agaaggcaaa gttagcggtt aaattcacaa
     2221 accacggcaa cggtttatca agctgaattt tcaaagtatg ggcatcaact gcggtgacgc
     2281 caagctgatc aggcgtagct ttaccatcaa taatcgcctg tgcgttgttg attcccgcca
     2341 gcgcggcaaa ccatgcaaat ggcgacaatg tttttgggtc caccagacgt tgccagctgt
     2401 agacaaaatc ttgcgccgtt accggtgtgc catccgccca ttttgcgtta tcgcgcaggg
     2461 taaaagtcca gatacggttg tcattacttt tccattgagt cgcgacgccg gggacaatct
     2521 cccctttttc gttctgattc accagacctt caaacagatc gcgaatgacc tgaatctctg
     2581 gcaggcccac ggctttagcg ggatccagcg acgcaggctc atctttaatg tggcgcacca
     2641 gctcctgctt ctctgccagt actgtgccgc tcggaacttc tgcagcatac gaaagagaaa
     2701 tgctgctgac caacagcgca caacacgtaa ctgaaacaga gtgcttcata agtttccctc
     2761 taaccgacat taaaattgat gcgtaattat ttgtttcgcc ttaaagaaat gcaaacaact
     2821 tccatcaaaa aagtgacaaa ggaagcatca aatgttttaa gtatcaatat atcaaatcag
     2881 atttagtgta aataatagac gccaaaatgt ggcaaagcac atagaacacc gaacactacg
     2941 ttcgggcttc gcacgttcaa accagttttc ggtatcataa tcagcatctg tcgccattgt
     3001 acgaagaata aaaaataacc aaaacgttga aaggaactga gaatacgtta agagaagaat
     3061 taaattaatg tatcagcaag tagtgctaaa cagcaccttt atacttcagc ctatctataa
     3121 ataatgagaa tgc
//
LOCUS       DAJTOW010000204         3037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTOW010000204 DAJTOW010000000
VERSION     DAJTOW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.206"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..198
                     /locus_tag="OO244_004001"
     CDS             <1..198
                     /locus_tag="OO244_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5665629.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="OO244_004002"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="OO244_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5665630.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="OO244_004003"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="OO244_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5665631.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="OO244_004004"
     CDS             1822..2577
                     /locus_tag="OO244_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5665632.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          790 a          679 c          719 g          849 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggagttatgt tcaacaattt
     2701 aattttagat atacatataa cttgttctat taataaatca cctcgtattc tgactcggcc
     2761 cctggcgcag caaagtcagg catttatact ctttttaaca catagaaaat tcatatcaat
     2821 ataataaaaa cataaaatat ttgcatatac atataatctt aaagtttagt ctatttaatg
     2881 ttcaatgaaa tatttctacc tgtataatct ttaaagatgt tgaacatata ttcacattaa
     2941 atatgattat gtacttgtta caaggataag gttataaatg aataaagtta caaaaacagc
     3001 tattgtgggc ttacttgcgc ttttcgcagg taatgcc
//
LOCUS       DAJTOW010000205         2945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTOW010000205 DAJTOW010000000
VERSION     DAJTOW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.207"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            80..907
                     /locus_tag="OO244_004005"
     CDS             80..907
                     /locus_tag="OO244_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5665633.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            951..1700
                     /locus_tag="OO244_004006"
     CDS             951..1700
                     /locus_tag="OO244_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5665634.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1697..2266
                     /locus_tag="OO244_004007"
     CDS             1697..2266
                     /locus_tag="OO244_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665635.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2390..2632
                     /locus_tag="OO244_004008"
     CDS             2390..2632
                     /locus_tag="OO244_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5665636.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2636..2782
                     /locus_tag="OO244_004009"
     CDS             2636..2782
                     /locus_tag="OO244_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665637.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2791..>2945
                     /gene="xisR"
                     /locus_tag="OO244_004010"
     CDS             2791..>2945
                     /gene="xisR"
                     /locus_tag="OO244_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5665638.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNP"
BASE COUNT          792 a          714 c          775 g          664 t
ORIGIN      
        1 ctggctatgc tctggcgtgt ctgaccccgc cctgcattta tgccatttat aagggtcttt
       61 ctttttctaa ggataaaaga tgaaaaaagt tattcctgct ttagcccttg ttctgctgac
      121 caccggactt gtcggttgcg atcgcgttga gcctggtaat gttggcatca aggtaaacaa
      181 actgggcgac gataaaggcg tcggagaagt ggtcggtgtt gggcgctact ggacgggatg
      241 gaacactgag gtttacatct tcccgacctt caaacaaatg aagacctacg atgagccatt
      301 cagcttccag atgagtgacg gtacaaccat cggctatcac atcggcgtgg cctacaaagt
      361 tgatccatcc aaagttacca cagtctttca gacctaccgc aaaggcgtgg acgacattac
      421 cgacaccgac ctgcgccaga agatagccga cgcactcaac cgactggcca gcaaaatgac
      481 caccgacaaa tttatcgacg gcggcaagtc tgagctgctg gatgcagctc ttaaagacat
      541 tcaggaagaa atgacgccca tcggtattca ggtaatgagc ctctcatatg tgggtaagcc
      601 ggagtacccg cctactgtta tcgacagcat taatgccaaa gtcacggcga accagaaaac
      661 cctgcaatgc gagcaggaag taaaacagcg cgaagcggaa gccaacatgt tgcgcgcgga
      721 agctgccgga caggcagatg cgattcgcac aaaagcccag gccgaagccg atgctattcg
      781 tttacgcggt gaagctctgc gccagaaccc aaacgttatg gagctggaag ccatcaacaa
      841 gtggaacggt acactgccgc aatacatgac cagcggtgcc aacacaccat ttattcaggt
      901 taagtaatac atatgcccgg tattacacgc cgggctgtct ggagataaaa atgaatactg
      961 taaccatcaa caacaaacag tttccggtaa tcgaatatcg cggtcagcgt gttgtgacgc
     1021 tggcgatgat tgatgaagtc caccagcgac cggaaggaac cgcgcgtgca gcattcaacc
     1081 gaaaccgtga gcatttcatc agcggtgtgg attatgccga attaggtgcg gacgtaatac
     1141 gtacggacct cccggaagga acattctcta aatttgcacc atcggggatt gtactttttg
     1201 aatcaggtta cctgatgttg acgaagccat tcaatgacgc ccttgcctgg caggttcagc
     1261 gcgaactggt taacagctac ttccgcactc gcacaccgct aacggaaatc gagatgatcg
     1321 ctgcgatagc cgccgacgct gttcgtcaac agaagcgcct gaatcatgtg gaagaacagc
     1381 tcgaaacagt cacagaagcg gtggaaacca tcaaacgcgg aaatatgcgc gccggatatg
     1441 tcggttaccg ccaggtggta gccaaaagtg gaatgagtga caccaagtgt cggaatctgg
     1501 tgaacgcata tcaaattcct acagacaccc acgaattcat gactccggac ggtctgttgt
     1561 ctcgcagggc tatcgtcgag cttgagccat ttatggcggc gttccaccaa atgatgtcag
     1621 aggctgaccc acgcggcaca cgctggtatc acccgaagat ggggcttttc caggtgatcg
     1681 gatgggagga taaagcatga tcatccagtc aaaacttatt cgcgccgctc tggtgtgcgc
     1741 tgctaaaaac gacgttcgtt attacctgaa cggtcttcac atcacgccga aacatattga
     1801 ggcaaccaat ggttccgtgg cactgcgcat ggctcacggc atccggacga agaaaaacat
     1861 cattgtccag ttcgaagggg gcgtcccggc caaagccgaa acgacagagc tgatttttag
     1921 taaagagccg atcgctgttc atcgcgacca gtttcagcga cgactgtcca ttaccggcat
     1981 taaactggtg gacggttgtt ttccggattt ggatcgcatc attccgaaaa agtttgaccg
     2041 ctgtactcac ccggtgctcc aggcgggtta cctgagttat ccagagaaga tgtttggtcg
     2101 tgagcgtaaa tttattcccg tccagttacg tccctccggt gacgggcaag cggtcagaat
     2161 tcagtttgat tccatcatca actcaatgta tggcaatcct gaatttgttg tgatgccttg
     2221 tcgtgatcat ggcgatttca acgtggctca ggagcatccg gaatgaaaat cgaataccag
     2281 gacgccgcag gaggtgaatc aatgagctgg cctgatgcaa tcgtaactct ggggatggta
     2341 ttcgcagtag cgtttgttgt gtactcgatt tgtcgatggg gataaccaca tgttcgcttt
     2401 gattcaacgc gggcagatat acacagacag agccggatac ccagtagaaa ttactcgcag
     2461 tactgagcac tcagtgttct ttcggaggat ggacggacgt accgggcgag tacgcatcgg
     2521 tgaattcagt agcctgttcg agcatattga ccacctggaa tatcacaaga ttctggctga
     2581 aacagaacaa gaaaagcacc tgaagaaatt acgcgcaatg caaaggaagt aaaaaatgaa
     2641 taaagcgttt gagctatggg tccaccagcg ttacggcaac cactacgacc tgacgcgaga
     2701 tgttgacggt ttctactgtc gtgaaattgt gaaacgaatg tttgaggtct ggtgccactg
     2761 ccgtgggctg agtgttgtgt gaggtaatgc atgggcaatg tgattcaact ggctcccaat
     2821 gaatgggttt gtgaaagcgt tctaatcgca attaccgggc tcaaaccagg cacaattctt
     2881 cggacccgga aagaatgctg gatggttgga agagagtata ttcacgtatc accagacggt
     2941 aatcc
//
LOCUS       DAJTOW010000206         2794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTOW010000206 DAJTOW010000000
VERSION     DAJTOW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.209"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..488
                     /locus_tag="OO244_004011"
     CDS             <1..488
                     /locus_tag="OO244_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5665639.1"
                     /translation="LKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVN
                     CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDDAES
                     GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            568..1263
                     /locus_tag="OO244_004012"
     CDS             568..1263
                     /locus_tag="OO244_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5665640.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1292..>2794
                     /locus_tag="OO244_004013"
     CDS             1292..>2794
                     /locus_tag="OO244_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5665641.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQ"
BASE COUNT          810 a          658 c          623 g          703 t
ORIGIN      
        1 ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt gctcttggtc
       61 actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt ccattcacta
      121 ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac gttgaagcgt
      181 ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc cctaatctgg
      241 caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt attgacgatg
      301 cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg atttatcaga
      361 tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac gtatcaaccg
      421 ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta acgctggatt
      481 accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc cttcctcttt
      541 ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt gatattaacc
      601 ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt acagcgaacg
      661 cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa caaaggcgca
      721 gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag ccgtgcccca
      781 tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc actgcgaatt
      841 attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat taatgttcgt
      901 gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac gctggtattt
      961 aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt aaacgatgcg
     1021 tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa cccaactgaa
     1081 tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag caaaattgaa
     1141 tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg aaagaacgtg
     1201 aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg tccactgcaa
     1261 taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc agggacataa
     1321 acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg tgctcactgg
     1381 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
     1441 ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc
     1501 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
     1561 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
     1621 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
     1681 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa
     1741 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
     1801 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
     1861 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
     1921 aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga
     1981 tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa
     2041 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacgcctt
     2101 tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
     2161 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
     2221 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     2281 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac aacgtcgctt
     2341 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     2401 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg aatttgctga
     2461 agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc
     2521 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     2581 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     2641 tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgttag
     2701 ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata cccgcaattg
     2761 gggctataac agtcgccaaa aaagtgaaat tcaa
//
LOCUS       DAJTOW010000207         2758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTOW010000207 DAJTOW010000000
VERSION     DAJTOW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.210"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO244_004014"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO244_004014"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5665642.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO244_004015"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO244_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5665643.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="OO244_004016"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="OO244_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5665644.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          636 a          754 c          744 g          624 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatg
//
LOCUS       DAJTOW010000208         2751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTOW010000208 DAJTOW010000000
VERSION     DAJTOW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.211"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            90..2612
                     /locus_tag="OO244_004017"
     CDS             90..2612
                     /locus_tag="OO244_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5665645.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2623..>2751
                     /locus_tag="OO244_004018"
     CDS             2623..>2751
                     /locus_tag="OO244_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5665646.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          733 a          592 c          692 g          734 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctagtcag tgacccgata aaaatgacgc
       61 tgtaagcaag ggataaggat aggcgtatta tgatgacgac cagaatagtg gttggcctca
      121 cggcagggac gtgtctgatt ttctcgcaaa acctgatggc cgaggtcagt gtattcaatc
      181 cggcgcttct ggaaatcaac catcaatccg gagtcgatat tcgccagttt aatcgggcaa
      241 acctgatgcc cccaggtgtt tatagcgttg atatttttat caacggtaaa atgtttgaac
      301 gtcaggatgt gacatttgtt caggataatc cagatgctga tctgcacgct tgctttattg
      361 ccattaaaaa aacactgtcc tcctttggca taaaagttga tgcgctcaaa tcgttcaatg
      421 atgtggatga gacggtttgc ctcgatcctg ctccacgtat tgaaggctca tcctggcagt
      481 ttgacagtga taaattgcag ctgaatatat ccattcacca aatctacatg gacgcgatgg
      541 cttatgatta catcagcccc acgcgttggg atgaggggat taatgcgctc accatcaact
      601 acgatttttc tggttcacat acactacgtt cagattatgg ttcacaagag acagatacca
      661 gttatctcaa tctgcgcaat ggactgaata ttggaccgtg gcggctacgt aattacagta
      721 ctttaaacac cagcgatggc cgtgcggaat acaactccat tagtacctgg atacagcgcg
      781 atattgccgc gttaagaagc cagattatga ttggtgatac gtggacggcg agcgatattt
      841 tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga taacgatatg ctacccgcca
      901 gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa gtccaacgcc accgtcatca
      961 ttcggcagaa tggctacgtg atttatcagt cagccgttcc acaaggtgct tttgagatca
     1021 ccgatctcaa caccgcaagt acaggtggcg atttggacgt aaccatcaaa gaagaagacg
     1081 gtagcgaaca acgattcacc caaccttatg cttcattggc gattcttaaa cgtgaaggtc
     1141 tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga agacggattt acaccggacg
     1201 tccttcaggc gcaaatactt catggttttt cccacgggat cactttatat ggaggtatgc
     1261 aggctgctga aaattatggt tctgcagctc tgggtgtcgg taaagatctt ggcgctttgg
     1321 gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt tagccatgat gatacagaaa
     1381 cgggtcagtc atatcgcttt ctctattcaa aactatttga cgacacagac actagcttgc
     1441 gcctggttgg ctatcgttac tccaccgagg gctactatac cctcaatgag tgggcatcgc
     1501 ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg acgtagtcgc gtggagggaa
     1561 cgctaacgca gtcgttgggg agagattatg gcaatttata cctgacatta agccggcaac
     1621 aatactggca taccgatgat gtcgaacgat taatgcaatt tggctacagc agtagctgga
     1681 agcgtctctc gtggaacgtc tcctggagtt attccaatac tgccagacag gggacgggga
     1741 acaaccatgc cagtgataac accagtgagc agatctacat gctctcttta tctgttcctt
     1801 tatcgggctg gtggggtaat agttacgcca cctattctgt ttcgcaaaac gataattccg
     1861 gtagctcaca tcaactcgga ctcagcggta cggcgctgga aagaaataac ctttcatgga
     1921 atttaatgca gtcctataac agtcatgatg atgaggttgg cggtaatatg tccctgacct
     1981 atgatggctc ttatggcacg gtgaacggca gctataacta cagccaaaat tcccagaggc
     2041 tgaattatgg tatcagaggg ggaattctgg cacacagcga aggggtaacg ttaagtcagg
     2101 agttaggtga aactattgct cttgttaaag cacctggggc cgccgggtta gaaatagata
     2161 atatgcgcgg tgctgcgacg gactggcgcg gctatacggt caagacacag ctaaaccctt
     2221 atgatgaaaa tcgggtagca atcagcgata actatttctc gaagtcgaat atagaacttg
     2281 ataataccgt cgttacgatg gttcccacgc gtggtgcagt ggttaaagcg gagtttgtga
     2341 ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc aaatggtaaa ccggtacctt
     2401 ttggagccat tgctgcgata caagatgcaa gtttggcaga ttcaggaatt gtcggtgacc
     2461 gtggcgaact ttatctttct ggtctaccag aaaaaggaca ggttacgtta tcctggggag
     2521 aaaacgcctc aacaaaatgc atcttcaatt attcattttc gacaccagaa agtgagagcg
     2581 gattaattga acagggtgtg acatgtcatt aaaaggattt ttatgaacaa gtatatcaaa
     2641 cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg tagcacttgc agctcctaaa
     2701 ggtatctgta cccctgataa tggcgtattt cacagtacgc ttgatttctc g
//
LOCUS       DAJTOW010000209         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTOW010000209 DAJTOW010000000
VERSION     DAJTOW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.212"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(64..822)
                     /locus_tag="OO244_004019"
     CDS             complement(64..822)
                     /locus_tag="OO244_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5665647.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO244_004020"
     CDS             complement(830..1933)
                     /locus_tag="OO244_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5665648.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO244_004021"
     CDS             complement(1943..>2572)
                     /locus_tag="OO244_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5665649.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTOW010000210         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTOW010000210 DAJTOW010000000
VERSION     DAJTOW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.213"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..557
                     /locus_tag="OO244_004022"
     CDS             <1..557
                     /locus_tag="OO244_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5665650.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNTAELRREMSHS"
     gene            515..1192
                     /gene="bglJ"
                     /locus_tag="OO244_004023"
     CDS             515..1192
                     /gene="bglJ"
                     /locus_tag="OO244_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5665651.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1230..2018)
                     /gene="fhuF"
                     /locus_tag="OO244_004024"
     CDS             complement(1230..2018)
                     /gene="fhuF"
                     /locus_tag="OO244_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5665652.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2159..2395
                     /locus_tag="OO244_004025"
     CDS             2159..2395
                     /locus_tag="OO244_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5665653.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2434..2520)
                     /locus_tag="OO244_004026"
     tRNA            complement(2434..2520)
                     /locus_tag="OO244_004026"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2484..2486),aa:Leu,seq:cag)
BASE COUNT          653 a          573 c          665 g          681 t
ORIGIN      
        1 ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat tattctttaa
       61 tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg
      121 ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga
      181 atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc gccatgctga
      241 cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt
      301 cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcat
      361 tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg cgtcgactgg
      421 caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac
      481 ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa ttaagaagag
      541 aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga gtttattcag
      601 aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt catttcagga
      661 tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca tgagaagtga
      721 gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc gtacccggcg
      781 tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc
      841 gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt
      901 gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt acattaacca
      961 aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc gtggctactc
     1021 aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag cgcataaatt
     1081 taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg ccgcagatat
     1141 tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt aatccgcatg
     1201 atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc acattgctgc
     1261 acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc gtcgcgcagt
     1321 accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc aaaaaagagg
     1381 gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc agtgagatac
     1441 cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc tccggtcgct
     1501 tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat tcgctgctgc
     1561 ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca ggcgacgcgt
     1621 ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc tttttcctgc
     1681 gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg tgcccatagt
     1741 gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat atgatcggaa
     1801 tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag cgtcatggcg
     1861 ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg atgtttggcg
     1921 atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg cgttcgccag
     1981 atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt aatctaaatg
     2041 ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc tctggcttgc
     2101 aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag atgaaaccat
     2161 gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc tgattgcagg
     2221 gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga tgggattagt
     2281 attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc taccatcgtg
     2341 cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg gatgacaaaa
     2401 cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga cttgaacccc
     2461 cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc
     2521 acagtcatct tacttttttt gatatcgcct cgtttggtgc gagggggggg ac
//
LOCUS       DAJTOW010000211         2528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTOW010000211 DAJTOW010000000
VERSION     DAJTOW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.214"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..192
                     /locus_tag="OO244_004027"
     CDS             <1..192
                     /locus_tag="OO244_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HCR5665654.1"
                     /translation="DNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWR
                     RHSWCGGLSPEQFENQNLA"
     gene            complement(224..769)
                     /locus_tag="OO244_004028"
                     /pseudo
     CDS             complement(224..769)
                     /locus_tag="OO244_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1423..2409)
                     /locus_tag="OO244_004029"
     CDS             complement(1423..2409)
                     /locus_tag="OO244_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665655.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          682 a          571 c          613 g          662 t
ORIGIN      
        1 gataatgcct gcgtggaaag cttctttcat tcgctgaaag tggaatgtat ccatggagaa
       61 cactttatca gccgggaaat aatgcgggca acggtgttta attatatcga atgtgattac
      121 aatcggtggc ggcggcacag ttggtgtggc ggcctcagtc cggaacaatt tgaaaaccag
      181 aacctcgctt aggcctgtgt ccatattacg tgggtaggat cagaattcca tccagtcgtt
      241 ttgggttgct aatgatgtcc tgcatccgct taatctggtc gggcgtagac atataacccc
      301 acagggtgag cagaaccttt tttgcggcta cactatttcc ttggtctgat actaatgcca
      361 tatagcccca taaactatcc ctcagccaga cagcatcata gtgggtttct gccgctgttt
      421 tgtcatcttc gaaagtagaa gcaagaataa acgttcccca gggtttctcc gtcaacgtta
      481 actttcgtgt gttttctgtt aatgttagca atccttgagt ttcgtctttg gtatagtgac
      541 tctttatttc attttttaat ttggctgagt gaatcagaag acgataatta ggatacttat
      601 ctggatttaa cgataaataa cttttatctg ttgctgcccc cctggaatta agaatgttaa
      661 atacagattc gcttgtcact tctgcctctg tcgcctggca tgtgccgcac gaaaatatac
      721 cagcgagcag aagagttaat gcagatttcc ctttcataaa ataatccatc aatcgtttgt
      781 taaaagcgta gataaccact tattcaacaa cttgaaaata aggtatttta cctgagttgt
      841 tacaaaagga ttgcattgcg taaacgcttt ttatttacaa aaaaatgggg aagtgttaca
      901 gcgattgcga aaagcctgaa gtgattgaat taagataata tagaaaatca gatacagcaa
      961 aaatggcaga ttgatcaggt gaagataaat tggagcgggc gaagggagcc gaggacaact
     1021 agctaacttc ttgtttctta aagattgatg aatccagtgg ttacagtgat ggttacacat
     1081 ggagaggctg aaggtcaaga cttgaagtac gtaacattcc cacaaaagga atagaacacg
     1141 gtcacggact acccggccct ttggtctgac tgagggtcgg gctatggggt aaatcgtcag
     1201 gctcattgta ttagataggg tctcacattt ttcagtcaaa actcatcgtc taatagccat
     1261 cagccaaaca acaagcgcta acttcagcag gaagaccccg agatagactt tgtgtaggtc
     1321 cattaggtcc tcctccttct gcacccatga actagatcac tagtacaaaa taaaggccag
     1381 acatccagtt acggaagtct gaccaatagt cgttattgtt agttagcttg tcttggtaaa
     1441 gctcaggcgt ttgactacag gcaggacagc agcagcgcta ccagtcccca tctttacagt
     1501 cgttgcagcc tcagcgacca cgaagacctt accgagaatc tcaaggtcgt gagactgggt
     1561 gttgccatct cgggacagga ccacagtaat gttctcgcca tcgtgacgga agcaaccgaa
     1621 gctcagagcc gtaaaacgct tagccatcat gaacacgcca cggtcttcac cgttgtaata
     1681 ggcgacgtca gtccccagca tatcgacgat agcgtcttca ccaccgaagc cctgcttagc
     1741 agcaagacgg gagagagcct tgaggtgtga ctcatgggcg ataatgccgt agtcagaaac
     1801 ctcagaaccg aaagaagact ccgtgttctc gtacagggca tccatgatag cgtcagcctt
     1861 agcggtcagg ctaccagtca gggccggaag ttctacggtc ttcagcagct ttgaggactc
     1921 cagcacgtca accatcttag agttaatggt acgaatcacg ttatcgcaga tgttcagtgc
     1981 gtcctgctgg agcgtcccct gagtgaaacc agtggtcgac ttctggtccc aaagctttgt
     2041 ttggtgagac acgaggatcg cctgttcaat cggctcaacg gtgatttcag tgccagacgg
     2101 agcggtcaca acttcaggtc gcttaccacc acggacaggg accagcacag agcggtcagc
     2161 cagagtgtga gcaatgattg gagtcggtaa gctgttacgg gtcggcagga cttctacagg
     2221 gaacgggatg ttctcttgag cgtctttaag tacaccgttc agcatagcct taacagtctt
     2281 agctaactgg agggcataga cctgagggtc ggcgttagtc ggcagacctt cattgacctg
     2341 aatgttgtcg gtatcggtga tgcgtactgg ctcaatgcca aactggaaag cgtttttagt
     2401 catattcatt ataattaatc tccttgagtc aaatacttga agtccctaac aggcagtcag
     2461 ggaccgtgtg ttctcagatg agggacgctt gcttacaagc tgtccatcca gttaccgcct
     2521 gactcaag
//
LOCUS       DAJTOW010000212         2524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTOW010000212 DAJTOW010000000
VERSION     DAJTOW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.215"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..254)
                     /locus_tag="OO244_004030"
     CDS             complement(<1..254)
                     /locus_tag="OO244_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665656.1"
                     /translation="MSLQVSHYNMLRASHEGSQEVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="OO244_004031"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="OO244_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5665657.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="OO244_004032"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="OO244_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5665658.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="OO244_004033"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="OO244_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5665659.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /locus_tag="OO244_004034"
     CDS             1250..1501
                     /locus_tag="OO244_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665660.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="OO244_004035"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="OO244_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1848..>2524
                     /locus_tag="OO244_004036"
     CDS             1848..>2524
                     /locus_tag="OO244_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5665661.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          653 a          595 c          662 g          614 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctacttcctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcgggtttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaacgtg cgggtattac
     1861 ttcgacctgt tctggttccg gaactcgggc tggtggtcct taagcccggt cgtgaatcca
     1921 tgccggtatt ccacaatacc cgggtactgg tggagccgga accgaaaagc atgcgtaatc
     1981 tgccgtccgg ggtcgttcct gccgttcgcc agccgctagt ggaagacaaa acattgctgc
     2041 cgtttttcag taacgcacgg gtgattcgtg ctgctggtgg tgctggcgca ttgtctgact
     2101 ggctgttgcg ccatattaaa tcctgccagt ggccacacgg cgattatcat cacagcgaaa
     2161 ccgtcattca ccgttatggt accggcgcaa tggtattgtg ctggcactgc gacaaccagc
     2221 tgcgtgacca gacatccgaa tcactcgagc aacttgctca tcaaaacctg tcagcatgga
     2281 tgattgacgt catcggtcac gcaataagcg gtacgcagga gcgtgaatta tctctggctg
     2341 aattatcctg gtgggcggtc cgcaatcagg tggcggacgc gctaccggaa gcggtattac
     2401 gtcgttcgct ggggttgcgt gcggaaaaaa tccgctcaat gtaccgtgaa agcgacatcg
     2461 tgccgggaga gcagaccgcc accagcatac tgaagcagcg cacaaaaaat cttgcgccgc
     2521 tgcc
//
LOCUS       DAJTOW010000213         2490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTOW010000213 DAJTOW010000000
VERSION     DAJTOW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.216"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..1558
                     /gene="kup"
                     /locus_tag="OO244_004037"
     CDS             <1..1558
                     /gene="kup"
                     /locus_tag="OO244_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5665662.1"
                     /translation="MGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSI
                     IVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHF
                     FLEYKTVSFIALGAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGA
                     LLLKNPEAIKNPFFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLS
                     PMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSI
                     LSTTVARQNWHWNKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMT
                     TWKSERFRLLRRMHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLK
                     HNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHR
                     CGLEGLSCRMMETSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNR
                     VIELGTQVEI"
     gene            1725..2144
                     /gene="rbsD"
                     /locus_tag="OO244_004038"
     CDS             1725..2144
                     /gene="rbsD"
                     /locus_tag="OO244_004038"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5665663.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2152..>2490
                     /locus_tag="OO244_004039"
     CDS             2152..>2490
                     /locus_tag="OO244_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5665664.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKI"
BASE COUNT          563 a          602 c          651 g          674 t
ORIGIN      
        1 tatggggcta atcggcggca gctttttcta tggtgaagtc gtcataacac ctgctatttc
       61 ggtgatgtca gccattgaag gtctggaaat cgtcgccccg cagctggata cctggatagt
      121 tcccctctca attatcgttc tcactttatt atttatgatt caaaagcatg gcacggctat
      181 ggtcggtaag ctgtttgcgc cgatcatgct gacctggttt ttgattctgg caggactggg
      241 gttacgtagc attattgcta acccggaagt gctgcatgcg ctgaatccga tgtgggcggt
      301 gcatttcttc cttgaataca aaacggtttc ttttattgca ttaggggcag tggtgctgtc
      361 gattacgggg ggcgaggtgc tgtatgctga tatggggcac tttggtaagt tctctattcg
      421 cctggcgtgg ttcaccgtcg tattaccttc cttgaccctt aattacttcg gtcagggagc
      481 gttgttgtta aagaacccgg aagcgattaa gaacccgttc ttcctgttgg caccggactg
      541 ggcgctgatc ccactgctga tcatcgccgc actggcgacg gtaattgcct cgcaggcggt
      601 tatctctggc gtcttctcat tgacgcgtca ggcggtacgt ctgggatatt tgtcgccgat
      661 gcgcattatt cacacctccg aaatggagtc agggcaaatc tatattccgt ttgtgaactg
      721 gatgctctat gtcgcggtcg tgattgtgat tgtcagcttt gagcactcca gcaacctggc
      781 ggcggcgtac gggattgcgg tgaccggaac catggtactg acgtctattc tctcgactac
      841 cgtggcacgt cagaactggc actggaataa gtattttgtt gcgctgatcc tgattgcttt
      901 cctttgtgtc gatattccat tgttcaccgc taacctcgat aaactgctct ccggcggctg
      961 gttgccattg agcctcggta ctgtgatgtt tatcgtgatg accacctgga agagcgagcg
     1021 tttccgcttg ctgcggcgga tgcatgaaca tggtaactct ctggaagcga tgattgcttc
     1081 gctggagaaa tcaccgcctg ttcgcgtgcc cgggaccgcg gtgtatatgt cgcgtgcaat
     1141 caacgtcatt ccctttgcgc tgatgcataa ccttaaacat aacaaggtat tgcatgagcg
     1201 ggtgattctg ttaactctgc gcaccgaaga cgcgccatat gtccataacg tccgtcgggt
     1261 acagattgaa caactgtcgc ccactttctg gcgcgtggtg gcaagttatg gttggcgaga
     1321 aacgccaaac gtggaagaag ttttccaccg ctgcggtctg gaaggattaa gttgccggat
     1381 gatggaaacc tccttcttta tgtcgcatga gtcgttgatc ctcggcaaac gcccgtggta
     1441 tttgcgtctg cgcggcaagc tgtacttgct gctgcaacgt aatgcgctgc gtgcaccaga
     1501 tcaatttgaa atcccgccaa acagggttat cgaactgggt actcaggtcg aaatctaacg
     1561 ccagacgcct cctttcttca taagggggcg tttttgtttt catggttaat caccatgtaa
     1621 aacgtttcga ggttgatcac atttccgtaa cgtcacgatg gttttcccaa ctcagtcagg
     1681 attaagctgc ggatcagcga aacgtttcgc tgatggagaa aaaaatgaaa aaaggcaccg
     1741 ttcttaattc tgatatttca tcggtgatct cccgtctggg acataccgat acgctggtgg
     1801 tgtgtgatgc tggtttaccc atccccaaaa gtacaacgcg tatcgatatg gcattaaccc
     1861 aaggtgtacc ttcttttatg caggtgctgg gcgtcgtcac aaatgaaatg caggtcgagg
     1921 ctgccattat cgcggaagag atcaaacaac ataatccgca actccacgaa acgttgctca
     1981 ctcaccttga gcagctgcaa aaacaccagg gaaataccat tgaaattcgt tacaccacgc
     2041 atgaacaatt caaacaacaa accgcagaaa gtcaggcggt aattcgcagc ggagaatgtt
     2101 ctccgtatgc gaatatcatt ctctgtgctg gcgtgacgtt ctgaggccgt catggaagca
     2161 ttacttcagc ttaaaggcat cgataaagcc ttcccgggcg taaaagccct ctcgggcgca
     2221 gcgttaaatg tctatccggg ccgtgtgatg gcgctggtgg gcgaaaacgg cgcgggtaaa
     2281 tccaccatga tgaaagtgct tactggtatc tatactcgcg atgccggcac gcttttatgg
     2341 ctggggaaag aaacgacatt taccgggccg aaatcttccc aggaagccgg gattggaatt
     2401 atccatcagg aactgaacct gatcccgcag ttgaccattg ccgaaaacat tttcctcggt
     2461 cgtgagtttg ttaatcgctt tggcaaaatt
//
LOCUS       DAJTOW010000214         2371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTOW010000214 DAJTOW010000000
VERSION     DAJTOW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.217"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..823)
                     /locus_tag="OO244_004040"
     CDS             complement(<1..823)
                     /locus_tag="OO244_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5665665.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="OO244_004041"
     CDS             complement(1422..1670)
                     /locus_tag="OO244_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5665666.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2025..>2371)
                     /locus_tag="OO244_004042"
     CDS             complement(2025..>2371)
                     /locus_tag="OO244_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5665667.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDSCSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          613 a          437 c          573 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctacactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggag tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg g
//
LOCUS       DAJTOW010000215         2326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTOW010000215 DAJTOW010000000
VERSION     DAJTOW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.218"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="OO244_004043"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="OO244_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCR5665668.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="OO244_004044"
     CDS             276..452
                     /locus_tag="OO244_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665669.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="OO244_004045"
     CDS             527..802
                     /locus_tag="OO244_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665670.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..2317
                     /locus_tag="OO244_004046"
                     /pseudo
     CDS             902..2317
                     /locus_tag="OO244_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
BASE COUNT          719 a          531 c          530 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggtag
//
LOCUS       DAJTOW010000216         2244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTOW010000216 DAJTOW010000000
VERSION     DAJTOW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.219"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(158..670)
                     /locus_tag="OO244_004047"
     CDS             complement(158..670)
                     /locus_tag="OO244_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5665671.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            865..1629
                     /locus_tag="OO244_004048"
     CDS             865..1629
                     /locus_tag="OO244_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665672.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1580..>2244
                     /locus_tag="OO244_004049"
     CDS             1580..>2244
                     /locus_tag="OO244_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5665673.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          643 a          490 c          565 g          546 t
ORIGIN      
        1 atcatcagcg accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt
       61 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
      121 ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      181 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      241 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      301 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa
      361 cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa
      421 ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga
      481 ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa
      541 ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa
      601 aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc
      661 actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt
      721 aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag
      781 cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa
      841 cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca
      901 taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg
      961 gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc
     1021 aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt
     1081 acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga
     1141 atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc attaaaacac
     1201 ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg
     1261 acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt
     1321 ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt
     1381 gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc
     1441 gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg caaggccgcc
     1501 actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat tgatgatggc
     1561 aatggagagc gtgacctcaa tgacttctac tctgacatcc aaaccgacgc tgaatccggc
     1621 tttacgtagt ttctggacta tgcgggcacg taacaaagtg ctttatggtg gtcggtcatc
     1681 gtcaaaatca tgggatgccg ctggcattgc catatttctg tcgaataaat acaccctgcg
     1741 tttttgttgt gcccgtcaga tccagaataa aatcgaagag tcggtgtata ccctgctcaa
     1801 aattcagata gacaggtttg gtctgcggca ccgtttccgt attctgaaca acaaaatcat
     1861 taaccgggtt actggctcgg aatttgtttt ttatggatta tggcgcaaca tcgaagaaat
     1921 taagtcactg gaggggatcg atatgttgtg gctggaagaa gcccacgcac tgacggaata
     1981 ccagtggaaa attctggagc caacgatccg taaagagggt tcggaatgct ggttcatatt
     2041 caaccccgga cttgttactg atttcgtctg gcgcaacttc gttgttgatc cgcccgaagg
     2101 cactctcatc cgcaaaatta actatgacga aaatccgttt ctgtctgaca ccatgcttaa
     2161 ggttatcgac gcggcgcgac gccgtgatcc ggatggtttt aaacatgtgt atgagggcgt
     2221 tccggagtct gatgatgatg cggc
//
LOCUS       DAJTOW010000217         2186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTOW010000217 DAJTOW010000000
VERSION     DAJTOW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.220"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..697
                     /locus_tag="OO244_004050"
                     /pseudo
     CDS             1..697
                     /locus_tag="OO244_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            699..1304
                     /locus_tag="OO244_004051"
     CDS             699..1304
                     /locus_tag="OO244_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5665674.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1291..>2186
                     /locus_tag="OO244_004052"
     CDS             1291..>2186
                     /locus_tag="OO244_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665675.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          508 c          610 g          536 t
ORIGIN      
        1 acgcccgtgc cggtgtgccg ggcagcgatg aggaaacccg tgcggacttt gaaaaccgcc
       61 gacgtaattc agttgcccgt aatgcccgga atattctgga agccatccgg ggtgaaatac
      121 tctccacggt agaaaacgtg gtggatgttt acgtcaccca taatccgaaa aaaacagaac
      181 aaaaagccgg agtcagtatc cattaacacc cggttcgctt tatgttggcg tgtacggcgg
      241 cagcccggca gatatcgcgg cggccatctg gcgtaaggct ccgccgggta ttgatatgaa
      301 tggcaacaca acgttcactg ttgcagataa ggagtacgat ccgccgtatc ctgaatacgt
      361 gatcagctgg cagacactca aacccgtcag tctgcatgtc agtgtgacgc tgaaaaaaag
      421 tgactacctg ccttcagata ttacccgaca ggtacagcaa tccgtgctgg acgcatttaa
      481 tggtacagat ggtggtctgc gggcaagagt tgcctctgtt gtctctgccg ggcgctacta
      541 tgccggcatt tacaaaaccg atccggaaca tattgatatt ctgggcctta ctgtgagccg
      601 tgatggttcg tcatggacaa cggctgtcac tttcgggata gatgagattc cggttctgga
      661 tgtgtcggat atcagtgtga aactccagga ggcataacgt gcagaatgtg gctgccactg
      721 tgcttgcgca gtatgctgcc agcccccgac tcaatgccct cattaacagc tttaacgcag
      781 cgctttcccc cgacagtttt atcagtgatt tttatggtct tatctggaac atcgataccg
      841 cagaaaagta tggtcttgat gtctggggaa agattgtggg tgtcagtcgc tggctgacgg
      901 taaaggacga ttttaattac ctgggcttca gcgagtccag gatggacacc ccggtaatgg
      961 atgatccctg tccgtttaat caggcaccgt tttacaacgg aaaatcggat acccggactg
     1021 ttgacctgtc tgatgctgta taccggcggc tgatactgat gaaagccatg tcgaacatta
     1081 ctgactgttc cgttccggat attaacctga tgctgagatt tatgttcgga aaaaaacgcc
     1141 gggcttatgt tctgaataat ggcgggctga ggatgagtta cgtctttgag tccgcgctct
     1201 cgttggcaga actggcgatt atccagtcgt cgggtgcact gccatccccg ccgggtgttt
     1261 atgtttcagt agttttaaag gagtcccgta atgaaggcca gtgataaacc ccgccagctg
     1321 gcggtcccct ttgcgagtac cggagataaa aaccgtatcc cggacaaggc gacacagcag
     1381 accagagaga gcggtaatgc tgcgtatgat tcaggttttc ctccggtgac catgacagcg
     1441 gtctcagcgg gaggtatacc gccacacggc aaggatttta acggtctgat gtacgatatt
     1501 accgcagcaa tacggttcgc ccaggctggc ggtttgtaca cgtataatgc cggttttgcg
     1561 ggggccattg gtggatatgc aaaaggagcc attctcgccg gagtcgcaac aacagcggtc
     1621 tggctgaata ccacggacga taacctgacc gatcctgaag gctccgacag tgcgggctgg
     1681 gtaaatcttc ttgaggatcc gaaaaggata ttcctgcggc agaagaacaa tctgtcagac
     1741 cttcagaata aagggacggc acgggataat cttcaggttt acagtaaaga gcagtcagat
     1801 caacgctatg ttcatcggga aggcgataaa ataaccggag agctgaaaat ccgtggtgtt
     1861 aatgcgctga ggattttcaa cgaagctttt ggcctgattt ttcgtcgttc ggaagagtgc
     1921 ctgcacctta ttcccaccag tgaaggtcag ggggaaaatg gcgatattgg tcccctgcgc
     1981 ccgttcacca ttaatttgcg gacgggtgaa atatccatgt cgcataaagt gtctgttggc
     2041 ggtggttcgc aggtcaatgg tgcgctgggt atcggcgttc agaacgccct gggggggaat
     2101 tcaattgttc ttggtgataa tgacaccgga tttaaacaga atggagacgg tattctggat
     2161 gtttatgcta atagtcagcg tgtatt
//
LOCUS       DAJTOW010000218         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTOW010000218 DAJTOW010000000
VERSION     DAJTOW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.221"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO244_004053"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO244_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5665676.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OO244_004054"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OO244_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5665677.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAJTOW010000219         2080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTOW010000219 DAJTOW010000000
VERSION     DAJTOW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.222"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            44..2080
                     /gene="sat"
                     /locus_tag="OO244_004055"
                     /pseudo
     CDS             44..2080
                     /gene="sat"
                     /locus_tag="OO244_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          758 a          364 c          414 g          544 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcatacg gcatgatgat
       61 ttataaaaat gataaaacat ttcgtaatct tgaaatattc ggagacagcg gctctggagc
      121 atacttatat gataacaaac tagaaaaatg ggtattagtc ggaacaaccc atggtattgc
      181 cagcgttaat ggtgaccaac tgacatggat aacaaaatac aatgataaac tggttagtga
      241 gttaaaagat acctatagtc ataaaataaa tctgaatggc aataatgtaa ccattaaaaa
      301 cacagatata acattacacc aaaacaatgc agataccact ggtactcaag aaaaaataac
      361 taaagacaaa gatattgtgt tcacaaatgg gggaaatgtt ctgtttaagg ataatttgga
      421 ttttggtagc ggtggtatta tctttgacga aggccatgaa tataacataa acggtcagag
      481 atttacattt aaaggagcag gaattgatat cggaaaagaa agcattgtaa actggaatgc
      541 attgtattcc agtgatgatg ttttacacaa aataggcccc ggtactctga atgttcaaaa
      601 aaaacagggg gcaaatataa agataggtga aggaaatgtt attcttaatg aagaaggaac
      661 atttaacaat atataccttg caagcggaaa tggtaaggta atactaaata aagataattc
      721 ccttggcaat gatcaatatg cggggatatt ttttactaaa cgtggtggca cgctagattt
      781 aaatggacac aatcagactt ttactagaat tgccgccact gacgatggaa caacaataac
      841 taactcagat acaaagaaag aagccgttct ggcaatcaat aacgaagact cctacatata
      901 tcatgggaac ataaatggca atataaaact aacgcacaat attaattctc aggataagaa
      961 aactaatgca aaattaattc tggatggtag tgtcaacaca aaaaatgatg ttgaagtcag
     1021 taatgccagt cttaccatgt aaggccatgc aacagagcat gcaatattca gaagcacagc
     1081 gagtcattgc tccctggtat ttctttgtgg aacggactgg gtcaccgttt tgaaagaaac
     1141 agagagttca tataataaaa agttcaattc tgattacaaa agtaataatc agcagacctc
     1201 atttgatcag cctgactgga aaaccggggt gtttaaattt gatacattac acctgaacaa
     1261 tgctgacttt tcaatatcac gcaatgccaa tgttgaagga aatatatcag caaataaatc
     1321 agctatcaca atcggcgata aaaatgctta cattgataat cttgcaggga aaaatattac
     1381 taataatggt tttgacttca aacaaactat cagtactaat ctatccatag gagaaactaa
     1441 atttacaggt ggcatcactg cacataacag ccaaatagcc ataggtgatc aagctgtagt
     1501 tacacttaat ggtgcaacct ttctgaataa tactcctata agtatagata aaggagcaaa
     1561 agttatagca caaaattcca tgttcacaac aaaaggtatt gatatctccg gtgaactgac
     1621 tatgatggga atccctgaac agaatagtaa aactgtaacg ccgggtctcc actacgctgc
     1681 tgatggattc aggctgagtg gtggaaatgc aaatttcatt gccagaaata tggcatctgt
     1741 caccggaaat atttatgctg atgatgcagc aaccattact ctgggacagc ctgaaactga
     1801 aacaccgact atatcgtctg cttatcaggc atgggcagag actcttttgt atggctttga
     1861 taccgcctat cgaggcgcaa taacagcccc caaagctaca gttagcatga ataatgcgat
     1921 ctggcatcta aatagccagt catcaattaa tcgtctagaa acaaaagaca gtatggtgcg
     1981 ttttactggt gacaatggga agtttacaac ccttacagtg gacaacctta ctatagatga
     2041 cagtgcattt gtgctgcgtg caaatctggc ccaagcagat
//
LOCUS       DAJTOW010000220         2073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTOW010000220 DAJTOW010000000
VERSION     DAJTOW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.223"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..368)
                     /locus_tag="OO244_004056"
     CDS             complement(<1..368)
                     /locus_tag="OO244_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5665678.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEA"
     gene            complement(407..694)
                     /locus_tag="OO244_004057"
     CDS             complement(407..694)
                     /locus_tag="OO244_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665679.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(944..1099)
                     /gene="hokD"
                     /locus_tag="OO244_004058"
     CDS             complement(944..1099)
                     /gene="hokD"
                     /locus_tag="OO244_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5665680.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1327..1992)
                     /locus_tag="OO244_004059"
     CDS             complement(1327..1992)
                     /locus_tag="OO244_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5665681.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          532 a          532 c          468 g          541 t
ORIGIN      
        1 gcctcaaacg actttagcaa tgccgtatca cgggttctgc gtgtcgcaac tgtattcaga
       61 tattgctctg cggcttcgct cagagctggc gtatgttccc ggcctactga ttcacacagg
      121 taatcaacga aaccggacac cagttttcgt tcgcgaggcg tgatcgcccc accgaccgga
      181 gtccagtaat cgaaacccag ttgcaggagt ttgaaaaaac gcttgtggaa tgcgtagtta
      241 cgcacacgct taaagtctgc gtgtatccac tcacctattt tgatttgatg cagaaaatcg
      301 caactctccg gcgtcgccgg gagaagtaat ccggaagagg tttgtttgac cagttgtata
      361 tgtgccatcg gctttctccg gtggcacggt gttacacagc aggagttcaa tcctgctcaa
      421 gattgtagat gagtttattc ttcagcaaaa gcagaaaacc agccttaaaa ccaatctttt
      481 tcaaaacccg taatgatgtg acaaattcgt cctcacgcaa aataaaaccg tccgtcagaa
      541 gtccattaca aaaataaaat aacacagcac cgctcttcct ttgttgagat tgcaaacatc
      601 taatgcggca atggctgaca atcgctccat tctcaacgcg cacagcatag aggccatttt
      661 cactaaaaat ttcacgcaat tcttcgattt tcatcttcag aatccttcca gataaatagc
      721 tctcccctgt tcggggtcca tccctcttct ccctgcgcgc tacttaagtg agtcgattct
      781 atctgcgaag gtgcgcgaat caaattcacc ggaaataaac aacaaaaaac ccgccgaaac
      841 gctgagagat cacagcgatg ggcgggttaa gtgcgggtgc gttgaggatg cctgacacat
      901 cagaggtggg cggggatggg atcagctccc cacccggtca ctcttacttc ctggattcgt
      961 agtctacgaa gacagcgacc tccgtctggc cggttcggat tcgtacctca cagaggtctt
     1021 tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattaaca
     1081 tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcatgtaa gaggctaacc
     1141 tacatgtgca aagcatgaaa ttggcctcag attaatgtta agcgtcttgc cggacgcgta
     1201 atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaag cagatagcct
     1261 caagcacccg cagtcattct acttacctcg ccaatatgaa atcaatcaga aaggtgcccc
     1321 ataaaatcac tccttctctt ctttaccgta gtggagttga ccaattttga taagagggcg
     1381 tccctgagat ttgcggtgta gattggtatc gcgcagagaa tacacacagc cacaatattc
     1441 ctgctgatag aatttttcgc gcttgctgat ttcaatcata cgggacgagc cgccctgctt
     1501 gcgccagtta taatcccagt acaccatgcc cggataatgc gcgacggctc gccgcccaca
     1561 gtcgttaacc tgctgcatat ttttccagcg tgaaatgccc agtgaactgc tgatcacact
     1621 gaaaccattt tcagcggcgt acagcgctgt ccgctcaaaa cgcatgtcaa aacacatggt
     1681 gcaacggatc cctcgttcgg gctcccattc cattcctttg gctcgttcaa accagttgtc
     1741 tgtgtcgtaa tcagcatcaa taaacggcac gccgtgttgt tcagcaaagc gaatattctc
     1801 atccttacga attaaatact ctttctgagg atgaatgttc gggttgtaga aaaagatggt
     1861 gtagtcgatt cccgaggcct gaagcgcctc catcacctca ccggaacatg gagcacagca
     1921 agagtgcagt agtagtttgt ttgccccgtt tgggagctcc aatttgggcc gtttgaaatc
     1981 agcaactgtc ataaatatgt ttattgaggt cataaaaata tcaaagagtg tagcattaga
     2041 acggggctat cggaaacaga tgtgtgactg ctc
//
LOCUS       DAJTOW010000221         2067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTOW010000221 DAJTOW010000000
VERSION     DAJTOW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.224"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            41..220
                     /locus_tag="OO244_004060"
     CDS             41..220
                     /locus_tag="OO244_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665682.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            272..466
                     /locus_tag="OO244_004061"
     CDS             272..466
                     /locus_tag="OO244_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665683.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1265..1601)
                     /locus_tag="OO244_004062"
                     /pseudo
     CDS             complement(1265..1601)
                     /locus_tag="OO244_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          654 a          432 c          427 g          554 t
ORIGIN      
        1 gcgtaaggca ctacagcgca ttgctttacg agtaataaca atggatttat caccagaaga
       61 cgcaaagttt ttacgagaac tgcaacaccg tatgctcaga gaagccatta tcgaacagca
      121 aacgggcatg agcgccatag acccattgcg atacaaacgg gcctcatctc ctttagttga
      181 cgacaattcc aatgcggata aaacacgtcc accacactga ttacattttt cattagcaca
      241 gtggtgtgat gcagcatacg aggaaaaccg gatgaatgag aacaaaataa aaaggctgga
      301 gcaactgtta caggcacgcc aacgggagtt tgccactaaa tggggacgtg ccgcgctgcc
      361 acatgaacgc ctaacctatt ttccacttga agacctcatt aaaaataatg gcaaaaaaat
      421 caggtctgat aagaaacact tcttagaatg caatgaagac aaataacaac aatctgcata
      481 ttgccaccaa ccacagatgt ggcatcgtta tcagcgcact tgattttctt tatattcact
      541 gacgagcttg ccggggcacc tttgtttgcc ggtcattctg gtatcatgca gatatttacg
      601 gcgtacaagc ccactagcat gacattgtaa taaggcctga gcctaacgct ttaatgtgtg
      661 tcatacactc agtggcgtaa tcgttgacaa tatggtgcat gtaatcgtaa gcgtccagcg
      721 aaatccatct ggtcataatc tgaagcatcc gacaaagtgg tgtccaccaa ataagtgatc
      781 tgcgcccaat atcctgagcc ggaactttga caaaggccaa cgaaaaatgg gagcggatgt
      841 tacagagttc tcggtacagg ggaaaagtgg tacctgtcgc cagttctccg atctgtttaa
      901 ccgggaaatt atctcctaca gcttgtcaga aaggtcggtg atggagcatc gttaatacga
      961 tgctatacga tgcattcaca gtgcccggcc agaggatgcc ccgtcgctgc atatggatca
     1021 gggttggcaa tatcgaatcg caggctatag gcaaagttaa agccccatgg agtagcacaa
     1081 aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tgaagaactt cttcagcaca
     1141 ctgctaaaac gcatagtgat cgagcgctga ttctggcgaa caactgaact aatacatcag
     1201 aatctgcatt atgttaaata aatatgaaaa gatggtttaa ataccccgtt acttttgact
     1261 tacactatac ggtatcgcat cgtttaatat tcgcaccggc cagattttta tttctattag
     1321 ttgtcacaat actgaatgcg tacgaccaca gtattctggc tcctgtgtgg ttatgcttta
     1381 attctgcgtt ccgggcagat aagcagttgc ttgcaggaat ccttcttgtg ttaatgtcag
     1441 ttcccctttt taccagtgct gatttccaca ttccgtccaa cagagcttat agcctttccc
     1501 tggattatag cattgtccgg ctgaagttct ttttgaataa taatagaagc actgctggca
     1561 gatccagtcc gtttttcata acccactgta ctgataacca taatctaatc agtagaaatt
     1621 gagtcgaaaa taagcactac tccatacagg ataattagag gtcagtttga ttattcacaa
     1681 ttcatcatca gcattttcta tttctgacga aatcaacatg aaaataacca tatatgataa
     1741 ttattataat aacggcttta attggaatac atatattaca acgtattata tataattggt
     1801 attctgggaa ctatattctc aaaatacagt agaaacgagg tatgtttctg gtggaaagga
     1861 cagtgggatt aaaaagtaag aattgataaa aaaaacgcca gcaacacata gtgccggcgg
     1921 agggaatacc ccatggagaa aatgtgatgc ctagagcatc atgaatatat aattaaaaat
     1981 agttagcgtt gtcaatactt tcacaaaaat aattttcgta gtatagaaag atatttttat
     2041 gcatgaccta cctgaatttg ctccggg
//
LOCUS       DAJTOW010000222         2021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTOW010000222 DAJTOW010000000
VERSION     DAJTOW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.225"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..352)
                     /locus_tag="OO244_004063"
     CDS             complement(<1..352)
                     /locus_tag="OO244_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5665684.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILM"
     gene            complement(419..1633)
                     /locus_tag="OO244_004064"
     CDS             complement(419..1633)
                     /locus_tag="OO244_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5665685.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASCEGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          630 a          489 c          409 g          493 t
ORIGIN      
        1 gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc attaatgacc
       61 agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc aaaataccgg
      121 aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca ccaaactctg
      181 tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat gacgaacggt
      241 cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc tgaatacgac
      301 gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc atttttagct
      361 ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg aaccgttatt
      421 accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc gggtcatacg
      481 gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca tcgcctgggt
      541 gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt tgttcggaat
      601 agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc agcgccgccg
      661 catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc tgataaagcg
      721 atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg atcgtcgtgc
      781 gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata ctgttaaaca
      841 cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa cattcctggt
      901 tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg ctcttcccaa
      961 agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga tgttcgttaa
     1021 aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga tacttttctg
     1081 aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac gccagtcctt
     1141 tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc acacgaatcg
     1201 ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca atcgtacgcc
     1261 cggtggtgtg gcaataaacc atcacgcctt cacaagaagc accaccaagc agttgataca
     1321 atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg gcagaaatgg
     1381 ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat tgccagatat
     1441 cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct ttcagataag
     1501 aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa agtccgcttt
     1561 cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa acatctgcag
     1621 caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa cctaatttta
     1681 cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca ttaaaaacaa
     1741 aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta accattattg
     1801 ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa aatgagcaca
     1861 gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg tatttaaaaa
     1921 tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag gaatgtgaca
     1981 aataatggtt ctggttgggc gttaaattta aggtcaccag a
//
LOCUS       DAJTOW010000223         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTOW010000223 DAJTOW010000000
VERSION     DAJTOW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.226"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(53..1702)
                     /locus_tag="OO244_004065"
     CDS             complement(53..1702)
                     /locus_tag="OO244_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5665686.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTOW010000224         1910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTOW010000224 DAJTOW010000000
VERSION     DAJTOW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.227"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(193..1797)
                     /locus_tag="OO244_004066"
     CDS             complement(193..1797)
                     /locus_tag="OO244_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5665687.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          483 a          477 c          430 g          520 t
ORIGIN      
        1 agagaaaact atgagtcaaa aacatgaccg cacaggtctg tagtaagtgt catcctagaa
       61 gagaaaactt tttgtgtccc ttctccctat agtgagtcgt attaattatc ccttgacaag
      121 tagagaaaac tatgagtcaa aaacatgacc acacaggtct gtagtaagtg tcatcctaga
      181 ctatggtctc ctctaggtct ccttataaac atcagccatc acattgacca gcctatcaac
      241 ctcgacctct ttagcataac ggtcgtacgt aatagaccct gaactatgcc ccatgatcgc
      301 ctgtgcatac gccaactcga ccccatgttg ttttaactgg gccgcaactg tatgtctaaa
      361 cgagtgaaac gctaagccgc tacggtctac gaggacttta ggcaacaacg tccgattaaa
      421 ccatttactc acctgctcac cgtaaccgtt cttcatgaga cgcagaccat cgaacaccat
      481 agcgtcatca ccgtcagcgc ctctacgggt ctctacaagc gccataaagt cagccaatat
      541 aaaaccatag gccccatcga ccagaggcac acagcggtca ctatgagcat tcttgatgct
      601 cttaccgggt agactactat cgtcttcatt gatatgaata taaacggttc ccgcctcagt
      661 ggtcctgacg tctttgacct gtagttgggc tatttcattg agtctcgccc cagtgatagc
      721 agccaaggcg gtaacataat aatgatacgg cttaccagag gtcttctgag agtatgcctt
      781 agctgcgacc agtagttgcc ccacctgctc cgtagagaag gcgttacggg cccctgaggc
      841 tttacgctga gggactttaa gctccagacc ctcagtcatg ttctttttaa tcaagtcatt
      901 gcgcacagcc cacctaaaga cagcagccat cttaataaga tacttgttgt tgatggtaac
      961 gacatccaga caatctgtct tatcttcacg gctcaacagg tcagcaagtg ggacatcctt
     1021 gaagcgctgc ttacggttcc taggtaactg ctggagcaca tcacggaccc taagcatgtc
     1081 agccctgtta gcgtctgcat tgagacccag atggtcgaat atttcgatta acgcagcgtg
     1141 tgacgccttg ttttccctta aggttgcagg cttccagttc tgatagttct ccgcttcgta
     1201 ttgctcaaag aggctcgcta tggtaatgct aggcgtaact tctggtttta cttctggagc
     1261 attaacagat agggacacag agtctgcttg gttatcttgc ctatcaatat cattaaaaag
     1321 atcgacgtaa tctatcagag gctgactgtc tcccgcttcg atacgcctca tacaggcttt
     1381 tagcaccttc agggcctcat tgatgtaacg atgctggtca atgcttagcg gctcactagc
     1441 cagagcatcg gtaaggctct caccaagttc tccgtattgg tcccggtaga tatccctcat
     1501 gtcaggctca aagagatctg aacggcccat agacagttca cactcagcga tgtccttaag
     1561 gtgttccctc atttcctcat aggtagcgtt gggtctgtct gcgtgtattg ccttgagtgc
     1621 tgcctttatg tgccttgagt aagccatagc ggtgcgtcta tccttggtcc gtaatgatat
     1681 tgaggtcatt ctatcattgt ttgattgtct gaccctaaga cggaagtagt agacgccgtt
     1741 acgctggtag agatagtaga ctgctggaag tttgtgacta ctggatagct gaatcatatg
     1801 tctgcatgcc ctgagagggt tacacagtgg gttacagtaa gtaactctgc aaggaggctt
     1861 aaagccgtat cccttaggat tcagtaggtt ggagtacttt aagaaaggtc
//
LOCUS       DAJTOW010000225         1867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTOW010000225 DAJTOW010000000
VERSION     DAJTOW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.228"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..837)
                     /locus_tag="OO244_004067"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="OO244_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          502 a          443 c          432 g          490 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcag
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttc
//
LOCUS       DAJTOW010000226         1861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTOW010000226 DAJTOW010000000
VERSION     DAJTOW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.229"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..345)
                     /locus_tag="OO244_004068"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="OO244_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(345..1265)
                     /locus_tag="OO244_004069"
     CDS             complement(345..1265)
                     /locus_tag="OO244_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5665688.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1403..>1861
                     /gene="pgrR"
                     /locus_tag="OO244_004070"
     CDS             1403..>1861
                     /gene="pgrR"
                     /locus_tag="OO244_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5665689.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          466 a          451 c          433 g          511 t
ORIGIN      
        1 acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta ctgcataatc
       61 gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac gaggctaccc
      121 accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt tttcagccaa
      181 tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag gatggacgct
      241 gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa tattggcagc
      301 aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag agtttcctca
      361 aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc ataaagatcc
      421 atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac
      481 aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg catcggctga
      541 gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt aaggctgcgc
      601 aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta atactcccag
      661 gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag tggcataatg
      721 gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc aatgtgtggc
      781 agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga accaatattg
      841 accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc attggctgta
      901 tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac ataagaaagc
      961 gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg gttttctaac
     1021 tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat agtgacaaat
     1081 cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac gcccccacct
     1141 gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg agggaaatag
     1201 atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac tttattattc
     1261 atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata tcactataga
     1321 tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt taaatgatta
     1381 atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt
     1441 cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca tggtgcagtc
     1501 agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc ttctgacgcg
     1561 aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg ttcttggccc
     1621 tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga accgcccatc
     1681 cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagccat
     1741 gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg attatggttt
     1801 gaccgatgtc gtttctgaac gttttgatgc aggcgtcctg aggtgtactg gcaatagcgg
     1861 a
//
LOCUS       DAJTOW010000227         1788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTOW010000227 DAJTOW010000000
VERSION     DAJTOW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.230"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..577)
                     /locus_tag="OO244_004071"
                     /pseudo
     CDS             complement(1..577)
                     /locus_tag="OO244_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            643..>1788
                     /locus_tag="OO244_004072"
     CDS             join(643..880,880..>1788)
                     /locus_tag="OO244_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5665690.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          483 a          434 c          438 g          433 t
ORIGIN      
        1 ttgacaacgc ccaaacttcg gctctttcgc ctttaccatc accagactgc caatcagtac
       61 ggtcagagtt tgcataagca ccagcaaatg agaaaccgtc aatagtgtaa gttgcagata
      121 aaccatggcc atcgccattt gctttgtagt tgcctgtacc ttcattttta ccctgatatt
      181 gcagagcaaa atttagacct tcaaccatgc caaagaaatc tgtgttacga taggttgcaa
      241 caccgttggt acggttggtc atgaacaggt cagtaccagc ccatgagtca ccgccccact
      301 caacaaacat atcagtaaat gcttctgcat catacgcgac accgacatta cgactgtagt
      361 cgaatgaacc aaaatctttg taactcaaac cagcatatgc caaacgagtt tggtcagggt
      421 tgtgtctgtt tgatgcttcc aggtctagtt caaactgacc gtaaccagtc atttcagggt
      481 tgatctgagt ttcacctttc acgcctacac gggcataaga ggtatcttga gaattatcgc
      541 ctttttcacc attttcacgg tcggtcagaa tgcgctcact gaatcgccac gggtttaaca
      601 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
      661 cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
      721 agcgttgcaa cacgtctcga tatcaccacc cacagccttt atgcctggat aaagaagtac
      781 ggtccggatt cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc
      841 cagaaagagc tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac
      901 ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg
      961 ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc
     1021 agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct
     1081 ggctggaatc gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgtgacagcg
     1141 ggcaacagtg cggagtaaac agagtctgga gactgatgaa acgtgtcgga ataaaggctc
     1201 aggtcggata ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca
     1261 ggctccagcg acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct
     1321 acatcaggac ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca
     1381 aaattatcgg ctggtccatg caatcccgga tgacaaagga cattgtcctg aacgcactgc
     1441 tgatggctgt atggcggcgt aatcccgaaa aacaggtgct ggttcattcg gatcagggca
     1501 gtcagtacac aagccatgag tggcagtcgt tcctgaaatc acacggcctg gagggtagca
     1561 tgagccgtcg cggtaactgc catgataatg cggttgcaga aagttttttc cagttgttga
     1621 aacgtgaacg gataaagaaa aagatctacg gaacgcggga agaagcccgc agtgatattt
     1681 ttgattacat cgaaatgttt tataacagta agcgtcggca tggttctagc gaacagatgt
     1741 caccgacaga atatgaaaac cagtattatc aacggctcgg aagtgtct
//
LOCUS       DAJTOW010000228         1779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTOW010000228 DAJTOW010000000
VERSION     DAJTOW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.231"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..360
                     /locus_tag="OO244_004073"
     CDS             <1..360
                     /locus_tag="OO244_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5665691.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO244_004074"
     CDS             361..726
                     /locus_tag="OO244_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5665692.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO244_004075"
     CDS             723..1406
                     /locus_tag="OO244_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5665693.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="OO244_004076"
     tRNA            1605..1680
                     /locus_tag="OO244_004076"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="OO244_004077"
     tRNA            1682..1758
                     /locus_tag="OO244_004077"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          490 a          376 c          492 g          421 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtagg
//
LOCUS       DAJTOW010000229         1707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTOW010000229 DAJTOW010000000
VERSION     DAJTOW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.233"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(110..577)
                     /locus_tag="OO244_004078"
     CDS             complement(110..577)
                     /locus_tag="OO244_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5665694.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO244_004079"
     CDS             complement(643..882)
                     /locus_tag="OO244_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5665695.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO244_004080"
     CDS             complement(1017..1622)
                     /locus_tag="OO244_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5665696.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          543 a          377 c          303 g          484 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcagaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacatt
//
LOCUS       DAJTOW010000230         1653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTOW010000230 DAJTOW010000000
VERSION     DAJTOW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.234"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(154..1389)
                     /locus_tag="OO244_004081"
     CDS             complement(154..1389)
                     /locus_tag="OO244_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5665697.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1536..1612)
                     /locus_tag="OO244_004082"
     tRNA            complement(1536..1612)
                     /locus_tag="OO244_004082"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1576..1578),aa:Pro,seq:tgg)
BASE COUNT          420 a          448 c          393 g          392 t
ORIGIN      
        1 caccgcacgt aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc
       61 aatttattga atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa
      121 tcaaccgcag gcggcttcca aatggccgct tttttactta ttcaccacca tcaaatgtgc
      181 ctgcataatg tcactggccg ggcgaccgtg cgccagcaaa tcagccattg ctttaagata
      241 tggcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt
      301 gccgctggca tcgagcatga agcggtgttt tgggcagcct ccccaacatg cttttaacac
      361 gttacaactg cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg
      421 ttgcggggaa tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg
      481 cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg
      541 cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc
      601 ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcaaaaat
      661 cgccaccaga aattcaccaa attcatcggg gcgcacagac cattccgtca gttccccctg
      721 aatatcacca ggcgcatgta gtttcagtcc atcgcggaca gctgtttcat cagccaggcg
      781 ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac
      841 ctgtaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg
      901 atgtttttgc aggagcgtca gggcacgcat caccagttta tgcgtgggtc tgccgccttt
      961 ggtcacgcga tattgattgt ggatctcagc cggaccatcc agcgataacc caacaagaaa
     1021 attattttct gccagaaatg cacaccattc gtcatcgagc agcacgccgt tagtctggaa
     1081 actgttactt atcttcctgc cagcaccata tttcgcctgt agcgccacgg cacggcggta
     1141 aaactccagc ccgagtagcg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
     1201 cagttcgctg gcggcgatat agtgacggat atacgcttcc agcgtgtcat cgtccatatg
     1261 cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
     1321 cagattacaa tcggaaccgc tcggtttcgc tatcacatga aaagcacgcg ttggaacctg
     1381 ttgcagcatg gtcgctcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata
     1441 ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag
     1501 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
     1561 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
     1621 ctttttgaat ttttagttca attctttaaa gtc
//
LOCUS       DAJTOW010000231         1638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTOW010000231 DAJTOW010000000
VERSION     DAJTOW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.235"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            81..995
                     /locus_tag="OO244_004083"
     CDS             81..995
                     /locus_tag="OO244_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5665698.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(999..>1638)
                     /gene="tam"
                     /locus_tag="OO244_004084"
     CDS             complement(999..>1638)
                     /gene="tam"
                     /locus_tag="OO244_004084"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5665699.1"
                     /translation="GPGNSTALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEAD
                     IRNWQPEQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLM
                     REVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTAT
                     GLRPWLQDLTESEQQLFLTRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
BASE COUNT          377 a          419 c          402 g          440 t
ORIGIN      
        1 aggtaatttg taagtagcgt cttgagaatg tgatcgtaat cacgtttaat agggcgactt
       61 ttccacgcct gaagataacc atgattgttc gtccacaaca acactggctg cgccgtattt
      121 ttgtctggca cggctcagta ttatccaaga tatcctcgcg cttactactc aattttctct
      181 tttctattgc tgttatattc atgctgccct ggtacacgca tctgggcatc aaattcaccc
      241 tcgcaccgtt cagcattctc ggtgtcgcca tcgccatttt tcttggtttt cgtaataatg
      301 ccgggtacgc ccgttacgtt gaagcgcgaa aactttgggg gcagttgatg attgcctcac
      361 ggtcgttact gcgagaggta aaaacgacat tgccggattc ggcaagtgta agggagtttg
      421 ccaggctgca aatcgccttc gcccactgtt tacgcatgac attacgcaaa cagccacagg
      481 cggaagtgct ggctcattat ctcaagactg gagatcttca gcgtgtactg gcttcgaact
      541 ctccagctaa ccgtatcttg ttaataatgg gagaatggtt ggcggttcag cgccgcaatg
      601 gacagctttc agatatcctg tttattagcc tcaacgatcg gcttaatgat atttcagcgg
      661 ttctggcagg ctgtgagcgc attgcctaca cgccaattcc ctttgcctac accctgattt
      721 tgcatcgtac cgtttatctg ttttgtatca tgctgccgtt cgcgctggtc gtggacctgc
      781 attacatgac gccttttatc tctgtgctga tttcctacac ttttatttcg ctggattgtc
      841 tggcggaaga actggaagat ccgttcggta ctgaaaacaa tgatttaccg ctggatgcca
      901 tctgcaacgc tattgaaatt gacctgttac agatgaacga tgaagcagaa attccagcaa
      961 aaattcttcc cgatcgtcat taccagctga cgtgataatt actccgtacg gcgggcaaca
     1021 ataaacagac gcggaaatgc cagcagtatc tgtccgttct cttgcagtgg atactgctct
     1081 tccagcatct gatggtagcg cgtaagaaaa agctgctgtt cgctctcggt cagatcctgt
     1141 aaccacggac gtaatccagt ggcggtcacc caatcgataa tcgcctggtg cgacggcatc
     1201 tggtgatagt aagtggttcg ccagatatcg acctcacatc cggcttcgct caaaatatcg
     1261 tagtaagcat gaacgccagc caacggctca cgcccgcgat ccgggtagtt ttgttcccaa
     1321 gcaacttcgc gcatgagcac atgggtcggc tccagccagt tatctggcat ctgtactgcc
     1381 agcacgccct gcggattaag taaagaaacc aagtgaggaa acaattcgta gtggtcgggc
     1441 agccattgca gtgaggcatt agcaaaaatc agatcgagtg cctgttccgg ttgccagttg
     1501 cggatgtctg cttccacgaa ctggcagtct ggcaaagcac tgcgcgcttc agcaatcatc
     1561 gccggagacg agtcgatgcc tgttatcctg gccgcaggcc aacgttgatg cagcagggcg
     1621 gtgctgttac ctgggcca
//
LOCUS       DAJTOW010000232         1587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTOW010000232 DAJTOW010000000
VERSION     DAJTOW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.236"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..626)
                     /locus_tag="OO244_004085"
     CDS             complement(<1..626)
                     /locus_tag="OO244_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5665700.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /locus_tag="OO244_004086"
     CDS             complement(740..1312)
                     /locus_tag="OO244_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5665701.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          472 a          334 c          316 g          465 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtgtttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTOW010000233         1582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTOW010000233 DAJTOW010000000
VERSION     DAJTOW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.237"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            494..1255
                     /locus_tag="OO244_004087"
     CDS             494..1255
                     /locus_tag="OO244_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5665702.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            1299..>1582
                     /locus_tag="OO244_004088"
     CDS             1299..>1582
                     /locus_tag="OO244_004088"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin outer membrane usher protein SafC"
                     /protein_id="HCR5665703.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYG"
BASE COUNT          485 a          253 c          354 g          490 t
ORIGIN      
        1 atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct ttttcgatga
       61 cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt tttgccacgg
      121 gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag agagttaata
      181 aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg ttttttaaat
      241 tttcaggttt tggttaaata tttgcttgca aatataggtg atttttttat tgtaggcttt
      301 tgaaggctat tataactgtg cgctaaatta atgcgttgaa taaaagaaat aacaaaatta
      361 agaaaagcca tatttacgat atgggcaaca taaatataat ctaaaaaaga gtaaggcgtt
      421 gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg gatttatcag ttgtcgaaat
      481 ggtttttata ataatgaaaa attttaatgt actaaccaaa gtcgtatttt gcttgtcgtt
      541 tttatcaagc gcatttccca cctatgctgc ggatgatgtc aaagtgactc aaaacacttc
      601 agtttttaaa ctgaagctta gtgcaacgcg attaatctat gaacaagggt cggatggcgc
      661 agcgataact gtgacaaatc cgcaggattg gccgatgctt gtgcagagta aagtctttga
      721 agaagataaa gtaactgcag cgccctttat tgtcactccg ccactgttca ggcttaatcc
      781 aggccagcaa aataaaatgt gggttgtgca gactgcgcca gctttacaag acagggaaaa
      841 attacactgg ctatgcgcca ccggcattac gcctaaaaag agtgatgttt gggccgagaa
      901 agataaagaa aaaattgctg aaaatgtgaa cctgaacatc caggttaacg tacatacctg
      961 cataaaactt attaccaggc catctggtat tgaaggcaag atggttaatg cgagtgagaa
     1021 gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc aatcagtcgc cgttctacat
     1081 caacctttct tctttacagg ttggcggtag caagattgag aaactagatt atattgcgcc
     1141 gttttcgcaa catgagtttg ttatgccaaa aactagcggc caggatatta aatggtctgt
     1201 gattactgat gaaggtggtg agagtcgttt gtttatatct catttgcaat cataatgtat
     1261 tacattttta ttgaatattt ttatgccatg catattccat gttttatcga ctgtcattaa
     1321 ttttgataat ggccttactt gcgggtcagt taagtgccca ggagtggtca tttgattctt
     1381 cacagttgga aggaaacgtt agtgcggaca ctgtggcaat gtttaatcag ggagagcaac
     1441 tccccggaaa ttatcgtgtg gaaatttatc ttaacggtga aaaagttgat gttggggagt
     1501 ttccctttca tcgtccggaa tcccctgaag aaaaagagct tgttccatgt ttaaccgttg
     1561 atgaccttat ccactatggc at
//
LOCUS       DAJTOW010000234         1559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTOW010000234 DAJTOW010000000
VERSION     DAJTOW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.238"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..427)
                     /locus_tag="OO244_004089"
     CDS             complement(<1..427)
                     /locus_tag="OO244_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5665704.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWW"
     gene            complement(493..1122)
                     /locus_tag="OO244_004090"
     CDS             complement(493..1122)
                     /locus_tag="OO244_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665705.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1126..1341)
                     /locus_tag="OO244_004091"
     CDS             complement(1126..1341)
                     /locus_tag="OO244_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5665706.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          415 a          414 c          330 g          400 t
ORIGIN      
        1 tccaccagcg aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa
       61 acgtcgacgg gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg
      121 ctttctgggg ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat
      181 cacgttcaat agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga
      241 caggcttacc atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac
      301 ggtatgccgt tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag
      361 cagacgcgcc tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg
      421 cgctcatgga tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc
      481 ttttaagata agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt
      541 attgaaatca cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag
      601 tgcttcaatc tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac
      661 tctggtgttt tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg
      721 atgcgatgcc cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga
      781 aaccctgaaa ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact
      841 tttatgctct tcctctccgg cactgttaac cgtaatgacg tactcaggcc gcaggtaagc
      901 cgcctctttt tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt
      961 ctcgtgagcg actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt
     1021 gttttcatct tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt
     1081 ttccaggtga ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc
     1141 agccttacgc ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc
     1201 cagaagcaga cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa
     1261 ccactgcaaa aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga
     1321 aattttgtcc atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca
     1381 ctgagaaatg aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac
     1441 agtaaggaaa gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg
     1501 cttatgaatg atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaa
//
LOCUS       DAJTOW010000235         1541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTOW010000235 DAJTOW010000000
VERSION     DAJTOW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.239"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..452
                     /locus_tag="OO244_004092"
     CDS             <1..452
                     /locus_tag="OO244_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665707.1"
                     /translation="DALEYATTRKKSEVVYSGVSVTIPTAPTNLVSLLKTLTPSSGTL
                     APFFDTVNNKMVVFNEDKTLFFKLSIVGTWPSGTANRSMQLTFSGSVPDTLVSSRNSA
                     TTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            452..907
                     /locus_tag="OO244_004093"
     CDS             452..907
                     /locus_tag="OO244_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5665708.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            910..>1541
                     /locus_tag="OO244_004094"
     CDS             910..>1541
                     /locus_tag="OO244_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5665709.1"
                     /translation="MLYAFKLGRKLRGEEPWCHEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGT"
BASE COUNT          409 a          398 c          395 g          339 t
ORIGIN      
        1 tcgatgctct ggagtatgcc actactcgca agaagtcaga ggttgtttac tctggcgtat
       61 ctgtaaccat cccgacagcg ccgaccaacc ttgttagcct gctgaaaacg ctcacgccgt
      121 catccggcac gttggcacca ttcttcgaca ccgttaacaa caagatggtt gtgttcaacg
      181 aggacaaaac cttgttcttc aagctgtcga ttgtcgggac gtggcccagc ggaaccgcca
      241 acaggtcaat gcagctaacc ttttccggct ctgttcctga cacactggta agcagtcgca
      301 actcggcgac aacgaccgat aacatcctgt tagctacgtt cttcagcgtg gataaagacg
      361 gctttcttgc cacaaatggc agtacgttaa ccattcagtc gaatggtgcg gcgtttactg
      421 ccacaaccat caagataatc gcggagcagt gatgattcag ttcaaaccaa cgcgaaacat
      481 cgacctgatc gaagcagtcg gaaatcaccc tgacattatc gccgggagca acaacggtga
      541 tggatacgac tacaagcctg aatgccgtta ctttgaggtg aacgtgcacg gtcagtttgg
      601 cggcattgtt tactatcagg agattcagcc gctgacatta gattgccacg ccatgtacct
      661 gccagagatt cgcggcttca gcaaggaaat tgggctggcg ttctggcgat acattctgac
      721 taacaccacc gttcagtgcg taacatcgtt cgctgcacgc aaattccgac acggtcagat
      781 gtactgcgca atgattggcc ttaagcgtgt aggaaccatc aagaaatact tcaaaggcgt
      841 ggatgacgtg actttttaca gcgccacacg cgaagaacta atcgacttcc tgaatcacgg
      901 gagatagcca tgttatatgc atttaagctg ggcagaaaac tgcgcggcga ggaaccttgg
      961 tgccatgaaa aaggcgggaa aggtggtagc tctgataaaa gcgcaaagta tgcagcagaa
     1021 gcccagaagt atgccgcaga cctgcaaaat cagcagtggc agacgatcat gaaaaacctt
     1081 gctccgttca cgcctcttgc ggagcagtat gttaaccagc ttcagaacct ttccagttta
     1141 gaaggtcagg ggcaggcact taatcagtat tacaactctc agcagtataa agaccttgca
     1201 gggcaggcgc gttaccagag tcttgctgct gcggaggcga cgggtggact tggttcgaca
     1261 gccacaagca atcaactggc tacgatcgcg ccgacactcg gtcagtcttg gttatcaaat
     1321 cagatgagca attacaacaa tctggcaaac gttgggcttg gtgcgctgca aggtcaggca
     1381 aacgccgggc agacgtacgc caacaacatg agcagcattg cacagcaaag cgcagctctt
     1441 gccgctgcta atgccaataa accatcaagt cttcagactg caattagcgg tggcacgtct
     1501 ggtgcgattg ccggtgcagg tcttgccagc cttttgggaa c
//
LOCUS       DAJTOW010000236         1500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTOW010000236 DAJTOW010000000
VERSION     DAJTOW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.240"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>1500
                     /locus_tag="OO244_004095"
     CDS             <1..>1500
                     /locus_tag="OO244_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5665710.1"
                     /translation="NERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYT
                     SYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAK
                     WRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDI
                     DSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPM
                     VQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFR
                     VNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRML
                     TSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNE
                     LAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTS
                     LTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAV"
BASE COUNT          449 a          340 c          364 g          347 t
ORIGIN      
        1 acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc acaaaaacat
       61 ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg
      121 ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg actacgcaga
      181 aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc gcaacctttg
      241 aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt gcccatcagg
      301 atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat acccgtaatt
      361 tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg gcacaattgc
      421 gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg gtcggcgata
      481 tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt ccggctaaca
      541 aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc tttaatatca
      601 ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg ccaatggtac
      661 aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta gttcagctgt
      721 tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt tctggcggca
      781 ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt gtaaatgcac
      841 gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca accattgatc
      901 agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc acctggctga
      961 aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt cgcctggcat
     1021 ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa ctttcgaaac
     1081 gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag ttaagaaaga
     1141 accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa aaagcattgt
     1201 ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg gcggcttaca
     1261 tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg gaaattagca
     1321 gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt gccaaggtta
     1381 ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta tcgaataaag
     1441 gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat aaagcagtta
//
LOCUS       DAJTOW010000237         1477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTOW010000237 DAJTOW010000000
VERSION     DAJTOW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.241"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..295
                     /locus_tag="OO244_004096"
     CDS             <1..295
                     /locus_tag="OO244_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5665711.1"
                     /translation="AHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLKTI
                     QDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            298..>1477
                     /gene="yiaN"
                     /locus_tag="OO244_004097"
     CDS             298..>1477
                     /gene="yiaN"
                     /locus_tag="OO244_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5665712.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     V"
BASE COUNT          274 a          385 c          383 g          435 t
ORIGIN      
        1 cgcccacgtt caggtcacct ttctggtgga gaaactctcc cccgcatggc aacgacgagt
       61 cgctctggtt acccattcct taatcttatt tatttgtggc gcactggcct ggggcgcaac
      121 gctaaaaaca attcaggact ggagcgatta ttcaccgatc ctcggtttgc ccatcggcct
      181 gatgtatgcc gcttgtctgc ccaccagcct cgttatcgca ttctttgaac tgcgtcattt
      241 atatcaactc atcacgcgta gcaattctct aacgtcgcca ccgcaaggag cttaatcatg
      301 gctgtgctga tttttctggg ctgtctgttg ggtggtattg ctatcggttt gcctattgcc
      361 tgggcactgt tgttgtgcgg ggcggcgtta atgttctggc tggacatgtt tgatgtccag
      421 atcatggcac aaacgctggt gaacggagcc gatagcttct ccctcctggc gattccgttc
      481 tttgtgctgg cgggtgaaat catgaatgcg ggcggcttgt taaaacgcat tgttgacctg
      541 ccgatgaaac tggtggggca taaaccaggc gggctgggct acgtcggcgt gctggcagct
      601 atgattatgg cgagcctttc tggttctgcc gttgcggata ccgccgccgt tgccgctcta
      661 ctggtgccga tgatgcgcag cgccaactac ccggtcaacc gggcggcggg gctgattgcc
      721 tctggcggca ttatcgcgcc aattattccc ccttccattc cgtttattat cttcggtgtt
      781 tccagcggat tatccatcag taagctgttt atggcgggca ttgcccctgg cataatgatg
      841 ggcgcaacgc tgatgcttac ctggtggcgg caggccagtc gccttaattt accccgccag
      901 caaaaagcaa cgatgcagga aatctggcac tcttttgtct ccggtatctg ggcgctgttt
      961 cttccagtca tcattattgg cgggtttcgc tccggtctgt ttacccctac cgaagccggg
     1021 gccgttgctg ctttctatgc gctgtttgtc gccacagtta tttaccgtga aatgaccttc
     1081 gccacacttt ggcatgtact cattggcgcg gcgaaaacca cctcagtggt tatgtttctg
     1141 gtggcctcgg cacaagtttc cgcctggctg attaccattg ctgaactgcc gatgatggtt
     1201 tctgatttac tgcaaccgtt ggtcgattca ccgcgtctgc tgtttatcgt cattatggtg
     1261 gctatcctga ttgtcggcat ggtcatggat ttaacgccga cggtattaat tcttaccccg
     1321 gtattaatgc ctttagttaa agaggcagga atcgatccga tttatttcgg tgtcatgttt
     1381 atcattaact gctcaatcgg cttaattaca ccgcctatcg gtaatgtact taacgttatt
     1441 tcaggggtgg caaaactcaa attcgatgat gcagtca
//
LOCUS       DAJTOW010000238         1446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTOW010000238 DAJTOW010000000
VERSION     DAJTOW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.242"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..382
                     /locus_tag="OO244_004098"
     CDS             <1..382
                     /locus_tag="OO244_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665713.1"
                     /translation="LWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNY
                     GNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSAD
                     NVDGVYYRPLQKLINGTWYNVASI"
     gene            385..930
                     /locus_tag="OO244_004099"
     CDS             385..930
                     /locus_tag="OO244_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5665714.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            954..>1446
                     /locus_tag="OO244_004100"
     CDS             954..>1446
                     /locus_tag="OO244_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5665715.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSE"
BASE COUNT          399 a          321 c          400 g          326 t
ORIGIN      
        1 tttgtggggt aatccatcca ggcctgttgt tgcagagctt ggtgatgatt caggctggca
       61 ttttttcagc cagagaaacc cggataacag catagtgttc actgttaacg gacaggtaat
      121 tccgttaaat tacggaaact tcgatgcccg ctataaatat cgaacagagg gggtacagga
      181 tgtacggtat ggccatgaaa tgtattacag ccccggcagt aacaccgttt cgtggagatt
      241 ttgcgcacct tcgggacacg ggctgtcagg gatggcgata tcggataccg gccgtaactc
      301 agcggataac gttgacggtg tgtattaccg accactgcaa aaactgatta atggcacctg
      361 gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactgc gggtaatccg
      421 aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa
      481 gacggaaaaa actggtatga agagctgaag aatttcgcca gcgacacaat aaaaatagct
      541 tacaccggag atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat cgagccacgc
      601 aatgccagcg ttatcgaagt tccggatatt accgctaacc gacggattac cgcgccgggt
      661 tactggtttt accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag
      721 cgtgatgccc ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg
      781 ctggggttaa tcagcgacga agataaagaa aagctgaaag cctgtcgtat ttacgcgaaa
      841 tcgctgcagg cgatggattt cagcaccatc actgataaat cctcatacaa cgccattgaa
      901 tggcccgcct ctccggaagg ttcttcctga tttaatttat cgcgagaaaa acaatgtctg
      961 tagtgatatc aggtgcgctg actgatggcg caggtatccc catgtccgga taccatatta
     1021 ttctgaaatc ccgggtaaac accccggaag tggtgatgaa cactgttgct gatgtgatgg
     1081 caggaaacga tggtgaatac tgtttccatg cgcggactgg aaaatatggt gtgtatctga
     1141 aacaggactg gcgcaacgag tacaacgttg gcgacattgc tgtatatgag gactcaaagc
     1201 ccggcacgct gaatgacttt ctgattgctc ctgatgaggg cgacctgaaa ccggatgtcg
     1261 tcaaacgctt tgaggaaatg gtggcgcagg cgcagcagag cgccggggcc gcagccggaa
     1321 acgcacagca gacggcgcag gatgtggcgg cagccgcagg ttatgcccgc gcagcagagc
     1381 aggccaaaaa tgacattgat gctgcgctga ccggcactct gaaaacggct aaccatctgt
     1441 ctgaaa
//
LOCUS       DAJTOW010000239         1367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTOW010000239 DAJTOW010000000
VERSION     DAJTOW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.243"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            39..>1367
                     /locus_tag="OO244_004101"
     CDS             39..>1367
                     /locus_tag="OO244_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5665716.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGS
                     PAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNAL"
BASE COUNT          381 a          338 c          382 g          266 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacccga
      361 tcgccagtaa gcctgaatcc ccgggacgaa aatcttcacg aaaaccgctg ccggcagaac
      421 ttccccggga gacacatcgc cttctgcctg ctgaaaccag ttgcccggcc tgtggaggtg
      481 ttctgaaaga aatgggggaa acaatctcag agcaactgga tatcattaat accgccttta
      541 aagttatcga aaccatacgt cccaaactgg cctgtagccg gtgtgatgtc atcgttcagg
      601 caccacttcc tcctaaaccg atcgaacgcg gttatgccag tgcagggtta cttgcacgga
      661 tcctggtcag caaatatatg gaacatatcc ctttatatcg ccagtcagaa atatacgcgc
      721 gacagggcgt ggagctgagc cgtaatacca tggtgcgctg ggtatcagaa atggcagaca
      781 aactccgtcc tctgtatata gcgctgaatg actatgttct ggaggcagga aaggtgcacg
      841 cagatgacac tccggtgaaa gtactggccc cggggaacgg aaagacgaaa acgggtcgtc
      901 tgtgggtata cgtcagggat gatcgtaatg cgggttcatc cctgccggca gccgtctggt
      961 tcgcgtattc ggcagatcgc aaaggagaac atccgcagct ccacctggca aagtatcagg
     1021 gcgtactgca ggctgatgcc tatgcaggtt ataacgtact gtacgaaacg ggccgggtga
     1081 aggaagccgg gtgcctggcc cacgcccgcc gaaaaatcca tgacgaggat gtgcgccgtc
     1141 cgacagaaat gactcaggaa gcgctcagac ggatagcaga gttatacgac atagaagcgg
     1201 agatacgtgg cagtccggca gaggaacggc ttgcagtcag aaaagccaga agcgtccagt
     1261 tgatgcagtc gttgtacgac tggatacagt tgcagaggaa aacgctgtcg aaacatgcgg
     1321 agatggcgaa ggcgttcgac tatatcctga atcactggaa tgcgctg
//
LOCUS       DAJTOW010000240         1298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTOW010000240 DAJTOW010000000
VERSION     DAJTOW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.245"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..187
                     /locus_tag="OO244_004102"
     CDS             <1..187
                     /locus_tag="OO244_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5665717.1"
                     /translation="GLPQHLFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIR
                     YMAETIAHHQVDIDLED"
     gene            193..1290
                     /locus_tag="OO244_004103"
     CDS             193..1290
                     /locus_tag="OO244_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5665718.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLEKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          404 a          297 c          315 g          282 t
ORIGIN      
        1 aggtctgcct cagcacctgt ttgaatcgct ctgcatcgac catttgcaac gccacggtgc
       61 caataaacag gcaatcagcc acgcatttga tgatgatgtt gagtttcagg agcgcatggc
      121 agaacacatc cggtacatgg ctgaaaccat tgcccaccac caggttgata ttgatttgga
      181 ggactaaaat ctatggcgct ttttcaacga gcaataaaca cacaggccta tctaaaagca
      241 ggaattatgg ggtttgctgg cgacggcaaa acctacacag caagcgaact ggctattggt
      301 ctggtaatgc ttatgcggca acggggaatt gaagctggta accgcccggt aatgtttctg
      361 gacacagaaa caggctctga ctgggttaaa ccccgctttg atgcggaaaa tatcgaatta
      421 tttacggcaa aaacccgctc gtttgttgat ctgcttgaag ccattaatga agcggaaagc
      481 agcggttccg taatgatcat cgactccatc agtcacttct ggaccggatt atgcgacgag
      541 tatgccagac gccgaaaccg taaacgagga ctggaatttt cagactgggc gtggctcaaa
      601 caggagtggc gacgctttac cgatcgattt gttaacagtc aggcgcacat tattatgtgc
      661 gggcgtgcag gatatgagta tgactttttc gaaggtgatg atggcaaacg ccagttggaa
      721 aaaaccggta taaaaatgaa agcagaaaca gaaaccggtt atgaaccctc catccggatc
      781 cagatggaga agcaaatgaa catagaaacc ggtcaggtgt ggcgcaccgc cagaatacta
      841 aaagatcgct ccacccgtat tgatggacag gtattttcaa acccaacatt taaaaacttt
      901 cttcctcata tcgagtctct taacctggga ggtgaacacc ccggcatcga tacctcacgg
      961 gataacagtg aattatttgc caacgacggt acaccgacat ggctaaaaga aaagcgtgca
     1021 aaagaaatcg cacttgatga aattatcgag ttgctcaaca agcaccatgg gggcaccagt
     1081 aacgacgcca aacgcgcaaa agctgatttg ctggaaaaaa cattcacctc ccgctcatgg
     1141 gaaagaatca aaggtatgga ctggccgaca atcaaagctg gtcgtaatgc actatggatt
     1201 gaactggaag gcgttgaata tgcattcccg gatccacaaa cacaaaacga aacgcaacaa
     1261 gcaggatacg atgagaatat tccggtgtaa aaccaagt
//
LOCUS       DAJTOW010000241         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTOW010000241 DAJTOW010000000
VERSION     DAJTOW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.246"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            34..435
                     /locus_tag="OO244_004104"
     CDS             34..435
                     /locus_tag="OO244_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5665719.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO244_004105"
     CDS             447..818
                     /locus_tag="OO244_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665720.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO244_004106"
     CDS             805..>1275
                     /locus_tag="OO244_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665721.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTOW010000242         1220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTOW010000242 DAJTOW010000000
VERSION     DAJTOW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.247"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="OO244_004107"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="OO244_004107"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5665722.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="OO244_004108"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="OO244_004108"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5665723.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..>1220
                     /locus_tag="OO244_004109"
     CDS             1134..>1220
                     /locus_tag="OO244_004109"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="catalyzes the formation of
                     (E)-3-(methoxycarbonyl)pent-2-enedioate and
                     S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and
                     trans-aconitate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5665724.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPL"
BASE COUNT          312 a          260 c          344 g          304 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg
//
LOCUS       DAJTOW010000243         1147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTOW010000243 DAJTOW010000000
VERSION     DAJTOW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.248"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..92)
                     /locus_tag="OO244_004110"
                     /pseudo
     CDS             complement(1..92)
                     /locus_tag="OO244_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            146..328
                     /locus_tag="OO244_004111"
     CDS             146..328
                     /locus_tag="OO244_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5665725.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(429..758)
                     /locus_tag="OO244_004112"
     CDS             complement(429..758)
                     /locus_tag="OO244_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5665726.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(930..>1147)
                     /locus_tag="OO244_004113"
     CDS             complement(930..>1147)
                     /locus_tag="OO244_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665727.1"
                     /translation="LVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPI
                     YGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          296 a          264 c          269 g          318 t
ORIGIN      
        1 ggccattcag gcagtatgct cagaatatgg cgcagatacg cttccggatc gataccgttc
       61 agacggcagg tgcctgcttt tccggcaaca agcaggaatc tcagcgctga agcaggaatt
      121 agaggtaaaa acgccctacc gggccatgaa tcacccggtc attggagtag taaccaaagc
      181 agatttagcc agcatggaac aaatatcttt ggtgaaaagt tggttacggg aagctggagc
      241 gcacaacgtg ttagtaacca gtgcagttaa taacaatgga gttacagaac tttttgcctt
      301 gctgcataca gaagaaggct gttgttaatt tcgattggtt ctcagatgag aatgatgacg
      361 gtgatacaca gataagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa
      421 aacgggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag
      481 atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct
      541 tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct catccctggc
      601 ttgatgtaat cgctcaggtt gtccagcaac aggacgcgct tctggttgtc acggatcttt
      661 ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat
      721 tccagtacgt cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga
      781 gagtcggatt cgtttaagca accgctattc gttagggcca acattactca tagctgccgc
      841 agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc
      901 agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc
      961 gatagcaact caagctgatg tgccgtttcc atgttcagcc acacataacc atagattggt
     1021 gtttccacga ctttcaggtc atggtggtta tcgagcaact gacgcagctc ttccacagca
     1081 tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga
     1141 acaaggc
//
LOCUS       DAJTOW010000244         1126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTOW010000244 DAJTOW010000000
VERSION     DAJTOW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.249"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..914
                     /locus_tag="OO244_004114"
                     /pseudo
     CDS             1..914
                     /locus_tag="OO244_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          329 a          194 c          276 g          327 t
ORIGIN      
        1 atttaagttc agcagcagtt aaaacagcag gtgtacttat cagctttcag gctgtactat
       61 ccgcttatca gcgagtaatg gaagttggcc ttcaacgtgc aagtgctgaa cgttcaattg
      121 atttcgtatt ttctaaagat gcaaatcaag taaaagattt cacaaagtca ttagctcaaa
      181 caacgggttt agatatcgct gaactgcaat cacagtttgc tggtttcggt gcttcagcta
      241 gagagtctct aggcattcaa ggttctgaag aactttttag aaatatgatt ggctattctc
      301 gtctgatggg gcgaagtgaa gaagagatta agcgagcact gacagcatta tcacagatgg
      361 ctggtaaggg tcagattatg gctgaagagc ttaaggggca actagctgaa gctgttcctg
      421 gtatggttca ggtattcgct aaagcaaccg gaaagactga acaggaatta tttgatgcta
      481 tgaagaaggg ggctttaaaa tctgccgata ctcttcagaa ggtcactcag gaactgaata
      541 aacaaatcac cgctaaaggt ggctggaaag ctatttcaga aagtacacag gcacaactag
      601 ggaacttgaa aaactcttgg aataccacgc ttgatagtat cttcagaggt tctgaaaatg
      661 gtctgcaaga ttttacaaga agcttaacca atcttttaaa cgctcttggt ggaagtggta
      721 agagtcttgg tgaatctctt ggtagtctca tgacttctat gtcacatggt gtcgattcat
      781 tgaccgatat tagttacagg gtaagggcgt tctttgatga ggtgacatta gcttaccgtc
      841 cactaaatga cactcagagg aaaattgttg atggtttatc tgatgggtta attgatggtc
      901 tgaagatgct tgctgtggct gttactgctc aaaaggctgc aaatgtagca ggaggcttgt
      961 taaaccttgc aaacgctgtt acttcattcg gtgacgctgt taataaagct aaagctggca
     1021 aggctggttc ttcaggtggt gcgctaaata aagtcggtag agttgtcctt tatgctgagt
     1081 tggtaaattt tgctcttgat aatttaaacc ctgaagcagc taagaa
//
LOCUS       DAJTOW010000245         1106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTOW010000245 DAJTOW010000000
VERSION     DAJTOW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.250"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..861
                     /locus_tag="OO244_004115"
     CDS             <1..861
                     /locus_tag="OO244_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5665728.1"
                     /translation="DVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTM
                     RGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETND
                     LKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLR
                     GVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQ
                     SEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFIN
                     RYGYLPSLGQ"
     gene            862..>1106
                     /locus_tag="OO244_004116"
     CDS             862..>1106
                     /locus_tag="OO244_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCR5665729.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMY"
BASE COUNT          307 a          289 c          289 g          221 t
ORIGIN      
        1 gacgtcgctc agatgtacca acagaacccg cggcagttcg gagaatttgt cgatcacctg
       61 gggatgaaca gtctcggacc cgaaaaatac tttgacctac aggataaaat gcagggtcgc
      121 caggttacca tgcgcggtca ggacctggat tcgcaaaccg ccgctcggaa tcaggcaatc
      181 acaatgcgcg ggcaagatat ccaggcgaat ttaggtcagc agcgcattaa tctggacgca
      241 gaaacaaacc gcattaacaa cgaaaataag cgccttgacc ggatgctatc agcagaaact
      301 aacgacctga agcgccagga aatacagagc cgcatagcag ccaacaacca gcagttgcag
      361 cagaagcagc aagcgctaaa tgatggctac aaagacggca tcaacactct cacaaccagc
      421 atgttcactc tgaacgatat cgttagttct ccttcactta agagcattac aggcttacgt
      481 ggagtaatcc ccaacgttcc aggctcacag gctgcagaca ctcaggcacg acttgatacc
      541 tttaaatcac aagcatacct gacagcggtt caggccatgc gaggcatggg cgcactttct
      601 gatgccgagg gcaaaaagct cgaccaggct gttggttcgc tgcagaactc gcagagcgag
      661 gagtcctttc gtcgcaacgc tggcgtcatc ctgaacacgc tcaaccagaa gcgtaatgag
      721 gcggttggta agtacgttca gcaaaacggt atcaagcgag tggaagcgcc tcaggcttct
      781 atagattacc tgaagcagca ccccgagctg tcaatcgact tcattaatcg ctacggatat
      841 cttccatctt tggggcagta aatggctaat taccgtgatt tgttagagca ggctggcgca
      901 cgttacggtg tgccagaagg gttgatgact gcactgggtg ccaaggagtc ttcttacaac
      961 cctgccgcag taagctccgc cggggctgta ggattgactc aggtcatgcc tgggacatgg
     1021 cgtgatatgg gttataccga tgagcaaatg caaaaccccg aatatcaggc tgacgctggc
     1081 gcgcgctatc tggcaaagat gtacca
//
LOCUS       DAJTOW010000246         1081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTOW010000246 DAJTOW010000000
VERSION     DAJTOW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.251"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO244_004117"
     CDS             complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO244_004117"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5665730.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(976..1052)
                     /locus_tag="OO244_004118"
     tRNA            complement(976..1052)
                     /locus_tag="OO244_004118"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1016..1018),aa:Asp,seq:gtc)
BASE COUNT          282 a          254 c          244 g          301 t
ORIGIN      
        1 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
       61 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
      121 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
      181 agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa tacgagcaat
      241 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
      301 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      361 gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca atgggatcgt
      421 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
      481 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      541 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      601 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      661 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      721 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      781 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      841 ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa acgcagagga
      901 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      961 aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
     1021 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
     1081 a
//
LOCUS       DAJTOW010000247         1073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTOW010000247 DAJTOW010000000
VERSION     DAJTOW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.252"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..146)
                     /locus_tag="OO244_004119"
     CDS             complement(<1..146)
                     /locus_tag="OO244_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5665731.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMS"
     gene            382..654
                     /locus_tag="OO244_004120"
     CDS             382..654
                     /locus_tag="OO244_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5665732.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(620..1027)
                     /gene="nudG"
                     /locus_tag="OO244_004121"
     CDS             complement(620..1027)
                     /gene="nudG"
                     /locus_tag="OO244_004121"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5665733.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
BASE COUNT          290 a          257 c          254 g          272 t
ORIGIN      
        1 gacatctggc gatattttgg attttgttca agaaaaggcc agagtgtggt cattacttca
       61 cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct tttggacatg gttgaggaat
      121 gactccagag aatatgtctg atccatagat ataaaaacct tatatattaa tacgattgtg
      181 cttatgttgt ttatgtcatg tgcttttgca gttttcgact ataccatcaa gaatgtgacg
      241 gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa cgagagtaat ctcataacca
      301 aatgcgataa ctcagacgct aaagttttgc cacccggcta ccgttatcag tagcagagac
      361 aataacgtta aggagttccc tgtgagtcga gcattgttcg ccgtagtatt agcatttcca
      421 ttaattgcac tggctaaccc gcattatcgt ccggacgtgg aggtaaacgt accgccagaa
      481 gtgttcagtt caggcgggca gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat
      541 caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa tactgctgca atgccagcgt
      601 gatgacaaaa cccttagcac taatccgctg ctctggcggc gcgtaaagcc ataaacgcct
      661 ctaataatgg aatgtcagca ggggccagcg gatattgcag cgcctcttca ggtgagcacc
      721 agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg
      781 catgaagatg gataatccgg cccgaaactt ctcgctgatg gctggcaaca tactcaccca
      841 cagttgcttc gatgtccaat tcttcgttta actcacgcac cagcgcctgc tgctggcttt
      901 catccagctc gactttacct ccggcaaact cccataatcc cgcctgatcg ctctgggcgg
      961 ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat ggcggcaaca acttcaatca
     1021 ttttcataaa ttacacacgg gcagataagc gaaaaatgcc ataatatccg cac
//
LOCUS       DAJTOW010000248         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTOW010000248 DAJTOW010000000
VERSION     DAJTOW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.253"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..544)
                     /locus_tag="OO244_004122"
                     /pseudo
     CDS             complement(1..544)
                     /locus_tag="OO244_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(612..>1001)
                     /locus_tag="OO244_004123"
     CDS             complement(612..>1001)
                     /locus_tag="OO244_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5665734.1"
                     /translation="AEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMG
                     INSQNVDEKAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVG
                     SESPLKIKRGQNNLWNNGGIQYAPPVR"
BASE COUNT          303 a          255 c          227 g          216 t
ORIGIN      
        1 aaacgcaata aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa
       61 aaaggccaga tcaaacgcgc tcacagcttg gcgtggtccg ggcaaattgc gcaacacggc
      121 aaagtaccag aagaagattt gcaatattac ggaagatctc aatataaatt gtggaaagct
      181 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
      241 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
      301 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
      361 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
      421 tttgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
      481 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
      541 gcatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac
      601 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
      661 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc aaaaatttcc
      721 gagtagttac tcacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc
      781 ttgccgtaat cgccttcttt acccaacaga tgtgccatat caggcgttgc tggattagcc
      841 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcatttag catggcgaaa
      901 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
      961 ggttctttag agataacctc cggtaagaca atccattcag c
//
LOCUS       DAJTOW010000249          923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTOW010000249 DAJTOW010000000
VERSION     DAJTOW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.254"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(45..233)
                     /locus_tag="OO244_004124"
     CDS             complement(45..233)
                     /locus_tag="OO244_004124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665735.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          244 a          177 c          214 g          288 t
ORIGIN      
        1 ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc tggtggtctg
       61 gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag ctccaatcgt
      121 tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg tactttttcg
      181 cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt cattgtctgc
      241 acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt attgttgcaa
      301 cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata acctgatact
      361 gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc ttttcatatt
      421 cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt aaatgtgaga
      481 catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat tcattctttc
      541 tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc atggtcagac
      601 aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac tgagtcttaa
      661 ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta atgattctgg
      721 caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg ttcgcgtcca
      781 ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac atcgcagata
      841 gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga attattttca
      901 atagacacct gcattacaca aag
//
LOCUS       DAJTOW010000250          912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTOW010000250 DAJTOW010000000
VERSION     DAJTOW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.255"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..180)
                     /gene="tnpB"
                     /locus_tag="OO244_004125"
     CDS             complement(<1..180)
                     /gene="tnpB"
                     /locus_tag="OO244_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5665736.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWAD"
     gene            complement(177..851)
                     /locus_tag="OO244_004126"
     CDS             complement(177..851)
                     /locus_tag="OO244_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5665737.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
BASE COUNT          214 a          227 c          234 g          237 t
ORIGIN      
        1 atcggcccac agtattttca ccattttgcc actgcggccc cggaagacga agatatgccc
       61 ggagaacggg tcatctttca gcgtgttctg caccttcgaa gccaggccat tgaagccaca
      121 acgcatatct gtgatgccag cgatgatcca gattttggta ccggtcggca gcgttatcat
      181 cggatacccc ctttcatttc gcggattagc gcccgtaaca gttccggagt gagagggtca
      241 aacagtttta ccacacctga tttaagatgc agctcgcacc gtgggacgtt tccgggagcc
      301 ccctcagggc gctcatcatg cttgttacgc cagaagggat ttgtaactgg tctggtcggc
      361 tccggcgtat cagtcagtgc caccgggaca ggcatgcatt cctgtatgtc atcatcgctc
      421 agtaagccgt cctcgtactg gcttttccat ttaaacagca ggttattatt gatatcgtgt
      481 tctctggcga tccgggcaac aacagcccca ggctgtaatg cctgcttagc cagacggacc
      541 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
      601 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      661 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      721 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      781 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      841 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      901 attttcacaa ga
//
LOCUS       DAJTOW010000251          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTOW010000251 DAJTOW010000000
VERSION     DAJTOW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.256"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..585)
                     /locus_tag="OO244_004127"
     CDS             complement(<1..585)
                     /locus_tag="OO244_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5665738.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
     gene            complement(585..>902)
                     /locus_tag="OO244_004128"
     CDS             complement(585..>902)
                     /locus_tag="OO244_004128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665739.1"
                     /translation="SKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFF
                     VDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVA
                     PPF"
BASE COUNT          214 a          264 c          229 g          195 t
ORIGIN      
        1 ccgtgttatc cggtagcgcc acatgctcat caccttacgg gcgtaaaaat gaagattttt
       61 ccaggtgtgg cccgacgtca caccaccggc agttgtcgat aaatggatat gcggatgcca
      121 ctgctggtca cgcccccatg tgtggatcac cgtgaatatc cccggctcca catctgcctg
      181 atggcagatt tccagtatca catccgctgc aatgcggctc atctctgcca gtaaccaccg
      241 gttgtggaac accagggacc agtactggca gggaagtgtg aacacaatat gctgccacgg
      301 gcagtagggg accaggctca gcagatactg tatccactgt gcgccagcct tcaccccgca
      361 gtgcgggcag gagcggcttt tacaccggaa gcagaccttt tttgtatggc aacagtccgg
      421 tgatgaacaa caccactgcg tgtagcccat cagtgtggtc ccgcaggcca tgatttttgt
      481 cactgattca attaccaccg gacggactgc cccttccggt tgtttctcca gccagttaag
      541 ccagcggttt ccctgctgaa aaatatcggc aaaacggggg agcatcagaa gggcggggcg
      601 actccgtccg gccagtgaac cgtgccacac tccgggcagt acataccgcc ggcgctgata
      661 ccggcaagaa tggtcgcaaa ttcccgctcc gtgcagcggg cgatttccgg atacccttcg
      721 tcatcaacac gtacaaacca gaagaccagc tttttgtttc ccgcatccac aaagaacgga
      781 atattcaggt ctgcgcagca ttcaacggca tcgtcaaaac tatcaaagcg cagaacttct
      841 gcgtcttctt cgtcaaaaaa atcatcttcg tgaagcttca cgacatagcg gggaagtttg
      901 ct
//
LOCUS       DAJTOW010000252          850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTOW010000252 DAJTOW010000000
VERSION     DAJTOW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.257"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..850
                     /locus_tag="OO244_004129"
                     /pseudo
     CDS             1..850
                     /locus_tag="OO244_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          204 a          216 c          270 g          160 t
ORIGIN      
        1 cgatatcgag gttgaggctg tcacaaaaat gctggcctgc ggcacgcgga tactgggcgt
       61 gaaggaatac atctgcgata aacctgagtg cccccacgtc agatacgtca ctaactcatg
      121 cggcagccgt gcctgcccgt cctgcggaaa aaaggccaca gacctgtgga tagcgacaca
      181 gctgaatcgt cttcctgact gcgactgggt acacctggtc ttcaccctgc cggacacgca
      241 gtggccggtg ttcgaaagca accggtggct gctgaatgac gtgtgccgtc tggcggtgga
      301 gaatctgctg tatgccgccc ggaaacgggg gcaggaaccc ggtatcttct gcgccatcca
      361 cacgtatggc cgtcgtctca actggcatcc gcatgtacat gtgtctgtaa cctgtggagg
      421 tctgaataag catggtcagt ggaaaaagct gagcttcctg aaagacgcga tgcgttcgcg
      481 gtggatgtgg aatatgcggc aacggcttct gaaagcgtgg tcagaggggc tggcgatgcc
      541 ggagtcgttg tcacatatca cgacggaatc acagaggaga agcctggtgc tgaaagccgg
      601 aggaaaatac tggcatgtgt acatgtcgaa aaaaacggcc ggcgggagga atacggcgcg
      661 ctacctgggt cgttatctga agaagccgcc gatagcggcc tcccggctgg cacattacaa
      721 cggaggggga gcctgagctt ccgttacctg gaccacaaaa cgggagaaac ggcgacggaa
      781 acgctgacac agcgtgagct ggtcgcgagg ctgaaacagc acatcccgga gaagtttttt
      841 aagatggtga
//
LOCUS       DAJTOW010000253          832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTOW010000253 DAJTOW010000000
VERSION     DAJTOW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.258"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            3..>832
                     /locus_tag="OO244_004130"
     CDS             3..>832
                     /locus_tag="OO244_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5665740.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
BASE COUNT          220 a          202 c          213 g          197 t
ORIGIN      
        1 ttatggctat aagcgacaca aaactgcgta ctatttatgg taaaccatat tctggcccac
       61 aagaagtggc tgatgctgat ggcctcagcg tacgaatttc accgaagggg gtcatccagt
      121 tccagtaccg ctatcgctgg catggcaagc ctaatcgact tgggcttggt cgatacccat
      181 ccctgtcttt gaaggatgcc agacagatca ctgctgactt gcgaaagctc tatttctcag
      241 gaacggatcc acgcacctat tttgaagaga aggtggagaa ctccatgacg gtcgcccagt
      301 gtctcgacta ctggttcgac aactacgtct ctacaactct cagagaaaag acccaggcac
      361 tttatcgatc aacggttatg aagcgcatgc atgacgcctt ccctaatcgt ccggcatctt
      421 ctatcacggt taagcaatgg gttgacctgc ttaccgaaga agaaaaagat aatccacgcc
      481 gagcaaggca ggtgctaagt caactaagat cagcaattag ttggtgcatg cggcgtcagt
      541 tgatagatag ttgcgcaatt atgagcatcc agccaaggga cttcggctcc cgcgctgagg
      601 tgggggatcg ggtactgtcg tatcacgaac tggctaagat ttggcttgct attgaaagaa
      661 gccgtgcgtc tacgtcaaat aagctccttc atcagatgct tatgctgtgg ggggcgaggc
      721 tctcagagct taggctggca acaaagacag aatttgacct gctggacaac gtatggaccg
      781 taccgaaaga gcatagcaag atgggtaatg ttatccgccg tccaatcttc ga
//
LOCUS       DAJTOW010000254          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTOW010000254 DAJTOW010000000
VERSION     DAJTOW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.259"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(41..100)
                     /locus_tag="OO244_004131"
     CDS             complement(41..100)
                     /locus_tag="OO244_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5665741.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..>829)
                     /locus_tag="OO244_004132"
     CDS             complement(339..>829)
                     /locus_tag="OO244_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665742.1"
                     /translation="KAVELTVRAKAEKEAAEMQAAAIVELAEATHKKGLAEAEAQRAL
                     NDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNSGSAAGDA
                     NTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLISTTPVAEN
                     VK"
BASE COUNT          181 a          223 c          197 g          228 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttgg
//
LOCUS       DAJTOW010000255          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTOW010000255 DAJTOW010000000
VERSION     DAJTOW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.260"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..825
                     /locus_tag="OO244_004133"
                     /pseudo
     CDS             1..825
                     /locus_tag="OO244_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          208 a          203 c          248 g          166 t
ORIGIN      
        1 gcattgaaat ggagaatgaa atattaaaaa aggctaccgc gctcttgatg tcagactccc
       61 tgaacagttc tcgataatcg ggaaactcag agcgcattat cctgtggtca cactctgcca
      121 tgtgttcggg gttcatcgca gcagctacag atactggaaa aaccgtcctg aaaaaccaga
      181 cggcagacgg gctgtattac gcagtcaggt acttgagcta catggcatca gccacggttc
      241 ggccggagca agaagcatcg ccacaatggc aacccggaga ggctaccaga tgggacgctg
      301 gcttgctggc aggctcatga aagagctggg gctggtcagc tgtcagcagc cgactcaccg
      361 gtataaacgt ggtggtcatg aacatgttgc tatccctaac taccttgaaa ggcagttcgc
      421 cgtgaccgag ccaaatcagg tgtggtgcgg tgatgtgacc tatatctgga cgggtaagcg
      481 ctgggcgtac ctcgccgttg ttctcgacct gttcgcaaga aaaccagtgg gctgggccat
      541 gtcgttctcg ccggacagca ggctcaccat gaaagcgctg gaaatggcat gggaaacccg
      601 tggtaagccc ggcggggtga tgttccacag cgatcagggc agtcattata cgagcaggca
      661 gttccggcag ttattgtggc gataccagat cagacagagt atgagccggc gcggaaactg
      721 ctgggataac agcccaatgg aacgcttctt caggagtctg aagaacgaat ggatgccggt
      781 ggtgggttac gtaagcttca gcgaggcagc tcacgccata acgga
//
LOCUS       DAJTOW010000256          814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTOW010000256 DAJTOW010000000
VERSION     DAJTOW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.261"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>814)
                     /locus_tag="OO244_004134"
     CDS             complement(<1..>814)
                     /locus_tag="OO244_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5665743.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGT
                     VVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALP"
BASE COUNT          196 a          231 c          201 g          186 t
ORIGIN      
        1 ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca
       61 aattctttgg cgtaatctgc cgctcgacca tcgcggctgg ctttgatttc gataagacgt
      121 gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca
      181 gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg
      241 gagccagaaa cggaaacgct catcccttca aaacccatac cgtggcgttt taacattgct
      301 tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact accgccaaat
      361 gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc
      421 gccataaagc cgacttcacg accaccaacg ccgatatcac ctgccggaac gtcggtatcc
      481 gcgcccaggt ggcgatacag ttcagtcatc agcgtctggc aaaaacgcat cactttacct
      541 tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat cggcagggta
      601 gtcagggcat ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga aaggttaact
      661 gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa ctgcacacgc
      721 catgcacggt tgacctgtat ctggttgcga tcatcaaccc ataccacgcg aaactggatc
      781 acgcgctccg gttcaaccag acgctccagt aatg
//
LOCUS       DAJTOW010000257          799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTOW010000257 DAJTOW010000000
VERSION     DAJTOW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.262"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            111..>799
                     /locus_tag="OO244_004135"
     CDS             111..>799
                     /locus_tag="OO244_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5665744.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          192 a          192 c          251 g          164 t
ORIGIN      
        1 agggtctgtt cgcgccgaaa ggtgttcggc ctgagacgtt gctccgacag gttaacaccg
       61 tctcgttgcc ggacagtgcg atcccgccag gcttcagggg gtaacagacc atgaaaaata
      121 ttgcgacagg aggcgttctg gagcgtatcc gcagactgac cccaccacat gtaaccgccc
      181 cattcagaac ggttgcggag tggcgcgagt ggcaacttgc tgaaggccag aaacgttgcg
      241 aggagatcaa ccgcctgaat cgtcagttgc gggtggaaaa aatcctgaac cgctccggca
      301 tccagccgtt gcaccggaag tgttcgtttg cgaattaccg ggcgcagaac gacggtcagc
      361 gacatgcact gagtcaggcg aaatccatag ctgacgaact gatgacaggc tgtacgaatt
      421 ttgtgttcag cggtaagccc ggcaccggaa aaaatcatct tgcagccgcg attggcaacc
      481 ggctgatggc gaaggggcgt agcgtgatta tcgtcaccgt gtccgatgtc atgagcgtgt
      541 tgcatgagag ctacgacaac ggcaaatccg gtgaaaaatt tttacaggag ctttgtggtg
      601 ttgacctgct ggtcctggat gaaattggca tgcagcggga tacgaaaaac gagcaggtgg
      661 tactgaacca gattgttgat cgccggacgg catcgttacg cggtgtgggg atgctgacaa
      721 atattaacca tgcagcgatg aatacacttc tcggcgagcg ggtgatggat cgcatggtca
      781 tgaacggcgg gcgctgggt
//
LOCUS       DAJTOW010000258          779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTOW010000258 DAJTOW010000000
VERSION     DAJTOW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.263"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            21..563
                     /locus_tag="OO244_004136"
     CDS             21..563
                     /locus_tag="OO244_004136"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665745.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
BASE COUNT          225 a          148 c          189 g          217 t
ORIGIN      
        1 ataacaccaa aggcgatgcc atgattggcg tcaagcagcc tttaccaaaa gcagttttaa
       61 gaactcagca tgacaaaaat aaagaagcaa taagtatcct ggattttggt gttattgatg
      121 atggtgtgac agataattac caggcaatac aaaatgctat agatgccgtt gcttcactac
      181 cctccggcgg ggagctgttt atccctgcga gcaaccaagc ggtggggtat attgttggat
      241 ccactttgct tattcctggc ggtgttaaca tcagaggagt tggtaaggca tcgcaactcc
      301 gagcaaaaag cggacttaca ggatctgtgt taaggctgtc ttatgattca gacactatcg
      361 gccgttatct gagaaatata cgagtaactg gtaataacac ctgcaatggt attgacacaa
      421 acattacagc agaagactct gtcatcagac aggtttatgg ctgggtattt gataatgtaa
      481 tggtgaatga agttgaaacc gcattattta atgcaagggc tctggcactc aaaatttata
      541 gcatgtcagg ctggaacctg tagagtcggt cttcactttt taggccagtg cgtaagtgtt
      601 agtgtcagct cctgccattt cagcagagga aattattctg ctgatgaaag ctttggcatc
      661 aggattcagc ctcaaacgta tgcgtggtcg tcagaggcag ttaggtcaga agcaataatt
      721 ttagacagtg agaccatgtg cattggtttt aaaaatgccg tctatgttca tgattgcct
//
LOCUS       DAJTOW010000259          772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTOW010000259 DAJTOW010000000
VERSION     DAJTOW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.264"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            27..299
                     /locus_tag="OO244_004137"
     CDS             27..299
                     /locus_tag="OO244_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665746.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            299..604
                     /locus_tag="OO244_004138"
     CDS             299..604
                     /locus_tag="OO244_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665747.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWNMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            604..>772
                     /locus_tag="OO244_004139"
     CDS             604..>772
                     /locus_tag="OO244_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5665748.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAAL"
BASE COUNT          247 a          170 c          184 g          171 t
ORIGIN      
        1 aagtaaatga aacagatgct gaatacctgg tgcgtaaatt cgatgagctt gaagctaaat
       61 gtgcagcaca ggaaaacaaa gtaataccag tgtcaactga actgccacca gcaaatgaaa
      121 gtgttttgtt attcgatgct aacggagaag gctggctaat tggctggcgt tctctctggt
      181 acacctgggg acaaaaagaa accggagaat ggcagtggac atttcaggtc ggggaccttg
      241 aaaacgtcaa tatcactcac tgggcagtaa tgccaaaagc accggaggct ggagcataat
      301 gaccactttt accgacaaag aactgattaa agaaattaaa gagcgtatca gcagccttga
      361 cgtgcgagac gatattgagc gccgtgctta tgaaatcgca ctcctatctc tggaagtaga
      421 accagatgaa cgcgaagctt atgaattatt catggaaaag cgttttggtg acttagtaga
      481 tcgtcggaga gcaaaaaacg gcgataacga atacatggca tggaatatga ctctcggttg
      541 gatcgtctgg cagcaacgag ctggtatcca tttctcaaca atgtcacagc aagaggtgaa
      601 ataatggagc catacagcct cacactcgat gaggcctgtc attttctcaa gatatccaga
      661 ccgactgcca ttaactggat acgcacaggg cgtcttcagg caacacgcaa agatcccact
      721 aagaataaat ctccttacct cacaacacga caagcctgca ttgcggctct tc
//
LOCUS       DAJTOW010000260          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTOW010000260 DAJTOW010000000
VERSION     DAJTOW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.265"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..246
                     /locus_tag="OO244_004140"
     CDS             <1..246
                     /locus_tag="OO244_004140"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5665749.1"
                     /translation="ELRLAFGVAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDV
                     APRSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ"
     gene            243..722
                     /gene="xdhC"
                     /locus_tag="OO244_004141"
     CDS             243..722
                     /gene="xdhC"
                     /locus_tag="OO244_004141"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5665750.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
BASE COUNT          200 a          191 c          212 g          160 t
ORIGIN      
        1 gaattacgcc tggcatttgg tgttgccgcg ccaacgccga ttcgctgcca acatgccgaa
       61 cagactgcac aaaatgcgcc attaaacctg caaacgctgg aagctatcag cgaatctgtc
      121 ctgcaagatg tcgccccgcg ttcttcatgg cgggccagta aagagtttcg tctgcatctc
      181 atccagacga tgaccaaaaa agtgattagc gaagccgtcg ccgcggcggg gggaaaattg
      241 caatgaatca cagcgaaaca attaccaccg aatgcaccat taacgggatg ccttttcagc
      301 ttcacgccgc accaggcacg ccgctctcgg aattactccg cgaacaagga ctgctaagtg
      361 tcaaacaagg gtgctgcgtg ggtgaatgtg gtgcctgtac ggtgttggtc gacggcacag
      421 caatagacag ttgcttatac cttgccgcct gggctgaagg aaaagagatc cgcacgctgg
      481 aaggtgaagc gaaaggcgga aaactttctc atgttcagca ggcttatgcg aaatccggca
      541 cagtgcagtg cgggttttgt acgcctggcc tgattatggc taccacggca atgctggcga
      601 aaccacgcga gaagccatta accattacgg aaattcgtcg cggactggcg ggaaatcttt
      661 gtcgctgcac ggggtatcag atgattgtaa atacagttct ggattgcgag aaaacgaagt
      721 aaaaggatat ccggcctgaa ttcaggccgg attcactgag gtt
//
LOCUS       DAJTOW010000261          761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTOW010000261 DAJTOW010000000
VERSION     DAJTOW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.266"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(50..190)
                     /locus_tag="OO244_004142"
     CDS             complement(50..190)
                     /locus_tag="OO244_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665751.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..>761
                     /gene="queE"
                     /locus_tag="OO244_004143"
     CDS             329..>761
                     /gene="queE"
                     /locus_tag="OO244_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5665752.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGG"
BASE COUNT          217 a          150 c          174 g          220 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc t
//
LOCUS       DAJTOW010000262          761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTOW010000262 DAJTOW010000000
VERSION     DAJTOW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.267"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..370)
                     /gene="arsB"
                     /locus_tag="OO244_004144"
     CDS             complement(<1..370)
                     /gene="arsB"
                     /locus_tag="OO244_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5665753.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVI"
     gene            complement(424..>761)
                     /gene="arsR"
                     /locus_tag="OO244_004145"
     CDS             complement(424..>761)
                     /gene="arsR"
                     /locus_tag="OO244_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5665754.1"
                     /translation="IQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          207 a          218 c          181 g          155 t
ORIGIN      
        1 cgatgacaat cggcgtcaaa ataagcgccg cgccatcatt ggcaaacagg gccgcaacgg
       61 cggcaccgag caggacaatc caggtaaaca gcaagcgacc gcgtccattg ccccagcgtg
      121 aaacgtgcag cgctgcccat tcaaaaaagc cggactcatc cagcagcagg ctgatgataa
      181 tgacggcaat aaacgccgcc gtcgcgttcc agacgatatt ccacaccacc ggaatatcac
      241 ccggatgaac cacgcccgta accaacgcca gtaccgcgcc gagcgttgca ctccagccga
      301 tgcctaaacc tttcggctgc cagataacca atacgatggt caggacaaag atagcgcctg
      361 ccagtaacat aatgcctccc ggataaaaca catgtgcaaa tccatatgtg tttagctaaa
      421 tttttaactg caaatgttct tactgtctcc ggaacagttt tgtctggcca ggttgcggac
      481 aattgcctga atcttttcct gttcacatcg ccaggcttca tcaataattt tcgccgccca
      541 tgctggaata tgcggcgata agcggtaatg aacccactta ccttgcttgc ggtccagcaa
      601 tagcccgctt tcacgcagca atgccagatg gcgggatatc ttgggctgcg actgatcgag
      661 ggcagtgcag agatcgcaga cgcataactc tcccagttcg ctaagcagta aaacgatgcc
      721 cagacgggtt tcatcagcaa gaattttgaa caattggatg g
//
LOCUS       DAJTOW010000263          749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTOW010000263 DAJTOW010000000
VERSION     DAJTOW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.268"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            163..>749
                     /locus_tag="OO244_004146"
     CDS             163..>749
                     /locus_tag="OO244_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR5665755.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLA"
BASE COUNT          190 a          176 c          195 g          188 t
ORIGIN      
        1 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
       61 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
      121 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
      181 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
      241 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
      301 aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga tgtttgtcgt
      361 ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc actcacagca
      421 aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg taatacgacc
      481 tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac ttattacgac
      541 ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga actggatggc
      601 gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga ctatttcaag
      661 gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc agcgaaggca
      721 ctggaatctg gtcgctgcga tacgctggc
//
LOCUS       DAJTOW010000264          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTOW010000264 DAJTOW010000000
VERSION     DAJTOW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.269"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>743
                     /locus_tag="OO244_004147"
     rRNA            <1..>743
                     /locus_tag="OO244_004147"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          182 a          162 c          242 g          157 t
ORIGIN      
        1 attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtatgaagaa ggccttcggg
       61 ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta atacctttgc tcattgacgt
      121 tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc
      181 aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt
      241 gaaatccccg ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga
      301 ggggggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg
      361 cgaaggcggc cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag
      421 gattagatac cctggtagtc cacgctgtaa acgatgtcga cttggaggtt gtgcccttga
      481 ggtgtggctt ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt
      541 aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat
      601 gcaacgcgaa gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt
      661 gccttcggga accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt
      721 tgggttaagt cccgcaacga gcg
//
LOCUS       DAJTOW010000265          732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTOW010000265 DAJTOW010000000
VERSION     DAJTOW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.270"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..732
                     /locus_tag="OO244_004148"
                     /pseudo
     CDS             1..732
                     /locus_tag="OO244_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          187 a          189 c          172 g          184 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa
      481 cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac cttttggccc
      541 ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct gggctgctat
      601 gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg acaatcattt
      661 ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat atgcattatt
      721 gctgctcaat ag
//
LOCUS       DAJTOW010000266          716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTOW010000266 DAJTOW010000000
VERSION     DAJTOW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.271"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>716)
                     /gene="ipaH3"
                     /locus_tag="OO244_004149"
     CDS             complement(<1..>716)
                     /gene="ipaH3"
                     /locus_tag="OO244_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5665756.1"
                     /translation="PGENRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVL
                     EITQNALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLE
                     YINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVR
                     NHHSEETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDY
                     HGPRIYFSMSDGQQNTLHRP"
BASE COUNT          178 a          124 c          205 g          209 t
ORIGIN      
        1 ggggcgatgg agtgtattct gttgtccgtc actcatggag aagtaaatcc gtgggccgtg
       61 gtagtccggt tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt
      121 gtcttcgagg atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt
      181 gatgcgattc tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac
      241 aggtacggct ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc
      301 aggtaactca gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt
      361 aggtaattca ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc
      421 aggcaattca ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc
      481 gggtaattca ggaagtgttg acaggcgatt gtcacaggcg tcaaggtatt ccagcgatgc
      541 tggcaattct ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg
      601 tggtaagttg tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg
      661 attgatgaga cattctttaa gtagggagac cgcttcattc cgattttcac cgggga
//
LOCUS       DAJTOW010000267          700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTOW010000267 DAJTOW010000000
VERSION     DAJTOW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.272"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            459..>700
                     /locus_tag="OO244_004150"
     CDS             459..>700
                     /locus_tag="OO244_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5665757.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          235 a          135 c          115 g          215 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatgcctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa
//
LOCUS       DAJTOW010000268          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTOW010000268 DAJTOW010000000
VERSION     DAJTOW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.273"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>697
                     /locus_tag="OO244_004151"
     CDS             <1..>697
                     /locus_tag="OO244_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR5665758.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGG"
BASE COUNT          175 a          203 c          199 g          120 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      541 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
      601 aaatgaagtg acagcccgcc tgctgaccgt gacgcgcaaa ggaaaagtct gccatctgct
      661 gacgtcgatg acggacgcca tgcgcttccc cggagga
//
LOCUS       DAJTOW010000269          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTOW010000269 DAJTOW010000000
VERSION     DAJTOW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.274"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..553
                     /locus_tag="OO244_004152"
     CDS             <1..553
                     /locus_tag="OO244_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5665759.1"
                     /translation="IYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGNM
                     FNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEALC
                     IDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWLN
                     LAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          179 a          145 c          199 g          170 t
ORIGIN      
        1 gatttaccca acaaatacag gcaaaagcgg ggaacacctt cgtctcacca cgctggaaag
       61 tgtctggatt cagggaaaac tgcgcatgtg ggggcgctgg tcgtatattg gcggcggtaa
      121 gacgggaaat atgtttaacc agttgctggc atccaaaaaa ttgacgaaaa cagccgtcaa
      181 tgaagccctg cgcaggatga aaaaagcggg aatagagaaa cctgagctgg aagcgttttt
      241 gcgagagatg atcaatggca agcaaaagac ctggctggcg cattgtactg atgcagaggc
      301 gttatgtatt gatagagtca taagtgaggt gctggcagag catcctggat tgattagtgt
      361 cctccggcaa cggtatgaag ggcgggggat gactaagcgt aaaatggctg aattgctgaa
      421 tgacgcacac cctgagtggt gtttccggac gtgctgcagt cgggtagatg tatggctaaa
      481 tcttgctgaa tatatgctct atctaccgat gcgtgatgca ttctcttccg gggatctaaa
      541 aaccgtttgt tgactcaatc tgttatccgg ggctatattc ctcacgcgcc agcaaaatct
      601 ggcgtcggga ttggcgtccc ggatgtttac ggagcgatat gagacgcgcc cgcgtctttt
      661 ttcatatcgt ttgcacagtc acattcgcga ttt
//
LOCUS       DAJTOW010000270          682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTOW010000270 DAJTOW010000000
VERSION     DAJTOW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.275"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            59..>682
                     /locus_tag="OO244_004153"
     CDS             59..>682
                     /locus_tag="OO244_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR5665760.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          199 a          175 c          156 g          152 t
ORIGIN      
        1 cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt aaaaaacaat
       61 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      121 cgtcctgtgg gcaagcgacg attttgaatc gaccagcacc actctggact atctgctcgt
      181 taagtcaggt aaaaaactga gcaactattt taaagctgtt gccacaaatt ttcctgtcgt
      241 taatgacctt ccccctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
      301 caaagactcc atgacatggg aactaaaacc gggagcagcg ccagacgacg ttcaccacca
      361 ggataatgct caagaaacca aagaactggc gggaggccag gaagaaaacg cgcaggcaga
      421 cgcccacgag gattgccagg attgcgaagt ctctgtagcc actttgcggt tcacacagcg
      481 tcttctgcac atttttacgt atgctgccgg ggatctgaaa tacctgcatc atgccacccg
      541 tgaacaacgc aaacacatta ctgctcttga gatggatcag gaaaacagct atgtccagaa
      601 tctgctgttg gccatacgcg gcatggcaga accgacaact ctggataatg ccgccctgct
      661 ccgcctgact gatgcaatta ag
//
LOCUS       DAJTOW010000271          669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTOW010000271 DAJTOW010000000
VERSION     DAJTOW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.276"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>669
                     /locus_tag="OO244_004154"
     CDS             <1..>669
                     /locus_tag="OO244_004154"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5665761.1"
                     /translation="KSSSDTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQD
                     KKSSLAPVSLWNEGVAAFRMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFR
                     SSVTWSKELGQSERWQRGYMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATD
                     DAMTPPEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGEL
                     DVTV"
BASE COUNT          195 a          123 c          172 g          179 t
ORIGIN      
        1 ataaaagcag cagtgatact gacaataaaa agaatcagtg ttttaaatgg aactctattg
       61 aaggtttgaa agtaaattat gattttgata gccagcgcgt acagataacc gttccacaac
      121 tgtatctgca agataaaaaa agtagtttag caccagtgtc gttatggaat gagggtgtag
      181 ctgcattccg catggtgtat cagacaaata ttgatatttc caaacaaaat gataatcaat
      241 ctaccacgcg taattctcgt tatggcaggt tcactccagg gtttaatctt ggtgcctgga
      301 ggtttcgtag ttcagtgaca tggagcaagg aacttggcca gtcagaaaga tggcaaaggg
      361 gttacatgtg gtttgagcga ggcataaatg cgattaaaag ccgattaacg cttggagaat
      421 cttatacgtc gtctgaggtg tttgacagca taccttttcg cggcgggatg ttagcgaccg
      481 acgatgcgat gactccgccg gaggacagct actacacacc tgtggtgcat ggaatagcac
      541 aaagtgaggc gcaggttatt attaaacaaa atgggcaaat tatttttacc cgttctgtgc
      601 cgccagggcc ttttgcgctg gataatttac caacgctggc cgttggcggg gagcttgatg
      661 ttacagtga
//
LOCUS       DAJTOW010000272          653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTOW010000272 DAJTOW010000000
VERSION     DAJTOW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.277"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..592)
                     /locus_tag="OO244_004155"
     CDS             complement(<1..592)
                     /locus_tag="OO244_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5665762.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLE"
BASE COUNT          164 a          166 c          156 g          167 t
ORIGIN      
        1 actccagtaa ctgcgcggcc acattttcga cttcctccgg agaggaaaat ttacggaaca
       61 ggatccagtt ccacagcaca ttcagtacag atttataaac ctgctgaaac tcggtttcgt
      121 ccatgttcgc aaatgcgata gattttgccc tgcgcccacg actaccatca ggataaatat
      181 gctcggtgta aaatccggcc tgaatggtta cccactcgcg gaaagcgtca aacgacttta
      241 gcaatgccgt atcccgggtt ctgcatgtcg caactgtatt aaggtattgc tctgcggcat
      301 cactcagggc tggagtgtgt tcccgaccta ctgattcgca caggtaatca acgaagcctg
      361 ataccagttt tcgttcgcga ggcgtgatcg ccccaccgac cggagtccag taatcaaatc
      421 ccagttgcag gagtttgaaa aaacgcttgt ggaatgcgta gttacgcaca cgcttaaagt
      481 ctgcgtgtat ccactcacct attttgattt gatgcaaaaa atcgcaactc tccggcgtcg
      541 ccgggagaag taatccggaa gaggtttgtt tgaccagttg tatatgcgcc atcgtagttc
      601 tccgctggcg cagtagaatg ggtgttcagc ccgttatgta gtataccaga att
//
LOCUS       DAJTOW010000273          645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTOW010000273 DAJTOW010000000
VERSION     DAJTOW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.278"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(142..639)
                     /locus_tag="OO244_004156"
     CDS             complement(142..639)
                     /locus_tag="OO244_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5665763.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          170 a          158 c          141 g          176 t
ORIGIN      
        1 cctgtgccat ttttcaggca caaaaaaacc cgcataaagc gggttctttc aagtatccat
       61 gtctgctatt cgcctcgcga tacagctttg cgaagcttac cgtaattgaa gcagtttatg
      121 tgcaaaaatg caagaacttt tttaaagctg catcaacctt tccaccagtt tatctctgcg
      181 aacaacaaac caaccattgg ctctcgccag ttccagccat gactcaaggg aaataacaat
      241 atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat aacagagaac
      301 tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcccttt gactgaaata
      361 gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca ttttgccgtg
      421 gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg aaactggatt
      481 ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa ctttgaaaaa
      541 gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa tacgcaccgg
      601 ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acagg
//
LOCUS       DAJTOW010000274          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTOW010000274 DAJTOW010000000
VERSION     DAJTOW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.279"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..643
                     /locus_tag="OO244_004157"
                     /pseudo
     CDS             1..643
                     /locus_tag="OO244_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          138 a          159 c          204 g          142 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca gtgaaacagt
      541 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc
      601 agtggagtgg ctgacggata atggttcatg ctaccgggct aat
//
LOCUS       DAJTOW010000275          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTOW010000275 DAJTOW010000000
VERSION     DAJTOW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.280"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..412
                     /locus_tag="OO244_004158"
     rRNA            <1..412
                     /locus_tag="OO244_004158"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            498..573
                     /locus_tag="OO244_004159"
     tRNA            498..573
                     /locus_tag="OO244_004159"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:532..534,aa:Glu,seq:ttc)
BASE COUNT          163 a          156 c          184 g          140 t
ORIGIN      
        1 gcggtccggc cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg
       61 acgtcaagtc atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca
      121 aagagaagcg acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg
      181 agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg
      241 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa
      301 agaagtaggt agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt
      361 gaagtcgtaa caaggtaacc gtaggggaac ctgcggttgg atcacctcct taccttaaag
      421 aagcgttctt tgtagtgctc acacagattg tctgatagaa agtgaaaagc aaggcgtctt
      481 gcgaagcaga ctgatacgtc cccttcgtct agaggcccag gacaccgccc tttcacggcg
      541 gtaacagggg ttcgaatccc ctaggggacg ccacttgctg gtttgtgagt gaaagtcacc
      601 tgccttgata tctcaaaact catcttcggg tgatgtttga gat
//
LOCUS       DAJTOW010000276          640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTOW010000276 DAJTOW010000000
VERSION     DAJTOW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.281"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..640
                     /locus_tag="OO244_004160"
                     /pseudo
     CDS             1..640
                     /locus_tag="OO244_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          161 a          182 c          175 g          122 t
ORIGIN      
        1 ccagtaaaaa accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg
       61 ctgaacgcat cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct
      121 acaactggcg cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt
      181 ctaccgagat tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc
      241 tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      301 acatcaggct gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      361 ctggtatacg tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca
      421 ctgcgacagc gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg
      481 cctgacggat gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg tggcggcaag
      541 cctgcgccgt cagggactga gggcaaaggc ctcccggaag ttcagcccgg tcagctaccg
      601 cgcacacggc ctgcctgtgt cagaaaatct gttggagcag
//
LOCUS       DAJTOW010000277          609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTOW010000277 DAJTOW010000000
VERSION     DAJTOW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.282"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..222)
                     /locus_tag="OO244_004161"
     CDS             complement(<1..222)
                     /locus_tag="OO244_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5665764.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGL"
     gene            complement(222..311)
                     /gene="kdpF"
                     /locus_tag="OO244_004162"
     CDS             complement(222..311)
                     /gene="kdpF"
                     /locus_tag="OO244_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5665765.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
BASE COUNT          209 a          137 c          129 g          134 t
ORIGIN      
        1 cagccccagc atgttcaggc cgagaatggc acaaagatat tgcttccagt tcatctcatg
       61 gtcagagacg ccaggtaaac gaaagagtat acgttcaacg cccgctgtac ctggaagagg
      121 aatgtcatta atcagccgcg ccagcccgct gcctaaagga cgcgccagca ccattaatac
      181 cagtaaaaac gtggcaatca gtaagaaccc ttgcgcagcc atcagaacgc ctccgcattg
      241 atcagggcat aaaccaaata gcccagtaat aaaaacacca gcaacacgcc ggctatcacg
      301 cctgcactca cagtgcacct ccagtggcct aaaagtgata ccggaagagt agaattctgg
      361 ctgcaaagat ttcgcaaaaa tctgcggacg gggtgtaaaa aaagtataaa aatggcaaag
      421 gccatgattt aactgatggt tagtattaat ttaacttttg tgtaatttaa ttacagtgtg
      481 aatgtaaata aaccatcaat aagcaaaaat aagtgatcgg atgagtagtg aaattacaca
      541 aaaggcggta ctattttcat cagataaaca aattcatttt tccggtgcta ttcaccgggt
      601 agatacaaa
//
LOCUS       DAJTOW010000278          608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTOW010000278 DAJTOW010000000
VERSION     DAJTOW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.283"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            392..>608
                     /gene="nanR"
                     /locus_tag="OO244_004163"
     CDS             392..>608
                     /gene="nanR"
                     /locus_tag="OO244_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5665766.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSV"
BASE COUNT          176 a          111 c          129 g          192 t
ORIGIN      
        1 agcattaccc attgttgaac tccgtgtcaa aagaaaacgg tcaatcccat aaatggcaga
       61 ttgaaaacaa tgatgttata ttttttgcaa gtctatttat ggtgcggatg tcgtgttttt
      121 aattgtaggt gaggtgattt ttcattaaaa aatatgcgat tatgattatt ttgtaagaac
      181 acattcataa tattcataat gcttgtgaat agtcttatga ataattcaat cgggatgtgt
      241 ttatctgcgt tacattaatt tttcgcaata gttaattatt ccgttaatta tggtaatgat
      301 gaggcacaaa gagaaaaacc tgccattttc ccctactttc aatcctgtga taggatgtca
      361 ctgatgatgt taatcacact gaccttacag aatgggcctt atgaacgcat ttgattcgca
      421 aaccgaagat tcttcacctg caattggtcg caacttgcgt agccgcccgc tggcgcgtaa
      481 aaaactctcc gaaatggtgg aagaagagct ggaacagatg atccgccgtc gtgaatttgg
      541 cgaaggtgaa caattaccgt ctgaacgcga actgatggcg ttctttaacg tcgggcgtcc
      601 ttctgtgc
//
LOCUS       DAJTOW010000279          589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTOW010000279 DAJTOW010000000
VERSION     DAJTOW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.284"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
BASE COUNT          182 a          144 c          115 g          148 t
ORIGIN      
        1 ccccgacaga cctgtgctat cggaaagaaa aaaggccatc agcaaaactc tgatgacctg
       61 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
      121 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      181 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      241 cctcctcccc ggaggggggc atttcattat ttttctaatc gttactggtg agaaggcgga
      301 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
      361 tttccagctc acacataccg agcgctgtac aaaaaaccgc cagccaaggc agttaagagt
      421 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      481 agaatgatga atcatcagtc ccctgccaga ggttcactac gtaacctgac cgacaatgca
      541 ccattctggt gctgaaaaaa gagcactgga actgcaacaa aacaacatc
//
LOCUS       DAJTOW010000280          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTOW010000280 DAJTOW010000000
VERSION     DAJTOW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.285"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..157
                     /locus_tag="OO244_004164"
     CDS             <1..157
                     /locus_tag="OO244_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2517 family protein"
                     /protein_id="HCR5665767.1"
                     /translation="YAVAAGVLALPFMLFWKDRARFYSYLHRVWSKTSDKPVWMDQAE
                     KATGDFY"
BASE COUNT          160 a          129 c          115 g          177 t
ORIGIN      
        1 atatgcggtt gcagcgggcg ttctggcgct gcctttcatg cttttctgga aagaccgcgc
       61 ccgcttttac agctacctgc accgcgtctg gtcgaaaacc agcgataaac cggtgtggat
      121 ggatcaggcc gaaaaggcaa ccggtgattt ttactgattg cctgcgcgct taagccagcc
      181 tcgtatcagg gggctggttt aggtgttcaa catcaacgtc ttccatgata attaacatgg
      241 aagacgtttc catcgtatta ttcatttcat cactgtttta gcatcacgtt tcaatagtat
      301 cctttcatag ggctacccag gatgcggtca ccttcattgt cgctctaagc gactagatca
      361 tcagaaatct ccagttagat tgcatacttc cttacacact ttcgtgaaac ttcagggtaa
      421 ggtgagaaag ccgtcatccc tttttctaat tccagcatct aaataaattc cgaattttgt
      481 tattagtatc gctaccgcaa taaaaacaga tagtaaagag agtaatatat actttattga
      541 tatattaaga ataattaatt gttaacatat ctaatccaat a
//
LOCUS       DAJTOW010000281          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTOW010000281 DAJTOW010000000
VERSION     DAJTOW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.286"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..124
                     /locus_tag="OO244_004165"
     CDS             <1..124
                     /locus_tag="OO244_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665768.1"
                     /translation="LSQKNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            137..562
                     /gene="arsC"
                     /locus_tag="OO244_004166"
     CDS             137..562
                     /gene="arsC"
                     /locus_tag="OO244_004166"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5665769.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
BASE COUNT          144 a          144 c          151 g          138 t
ORIGIN      
        1 actttcgcag aagaatatga ctatcagctg gggatattac ttccgtacag ggattatcat
       61 gaccctgcct gtgctgtttg tgacgctggc tgcgctggcg ctacgtctct ctttcacttt
      121 gtaatgagat actgatatga gcaacattac catttatcac aacccggcct gcggcacgtc
      181 gcgtaatacg ctggagatga tccgcaacag cggcacagaa ccgaccatta tccattatct
      241 ggaaactccg ccaacgcgcg atgaactggt caaactcatt gccgatatgg ggatttccgt
      301 acgcgcgctg ctgcgtaaaa acgtcgaacc gtatgaggag ctgggccttg cggaagataa
      361 atttactgac gatcggttaa tcgactttat gcttcagcac ccgattctga ttaatcgccc
      421 gattgtggtg acgccgctgg gaactcgcct gtgccgccct tcagaagtgg tgctggaaat
      481 tctgccagat gcgcaaaaag gcgcattctc caaggaagat ggcgagaaag tggttgatga
      541 agcgggtaag cgcctgaaat aaagcggcga tatccac
//
LOCUS       DAJTOW010000282          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTOW010000282 DAJTOW010000000
VERSION     DAJTOW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.287"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            329..>566
                     /locus_tag="OO244_004167"
     CDS             329..>566
                     /locus_tag="OO244_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="catalyzes the transfer of heptose(III) to
                     heptose(II) in the lipopolysaccharide inner core; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5665770.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHL"
BASE COUNT          172 a          108 c          109 g          177 t
ORIGIN      
        1 atagcaattt acccgccaat tcttgctgaa catctatact ttttttgtat ttttatgcga
       61 ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga aagaatatgg
      121 ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg cttaaaaatc
      181 attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc agtgcggtat
      241 cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct tcaccttaat
      301 cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa ttttgctcat
      361 taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt ctctgaaaaa
      421 aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc cgatcctgtc
      481 tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga
      541 aaaaattgcc aacttttttc atctca
//
LOCUS       DAJTOW010000283          563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTOW010000283 DAJTOW010000000
VERSION     DAJTOW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.288"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            9..>563
                     /locus_tag="OO244_004168"
     CDS             9..>563
                     /locus_tag="OO244_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5665771.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRW"
BASE COUNT          207 a          115 c          134 g          107 t
ORIGIN      
        1 gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga
       61 acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc
      121 atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa
      181 cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg
      241 atgacaaaag ccggatcgga ttctggcttc aacatttcgc aggaatacaa ctaagagaca
      301 ttactgaatc aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa
      361 actggaaact cagggcagaa tcatgcagaa aaaaagggaa acctgttcca gaatacacgc
      421 caaaaccagc gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa
      481 gagccgcaga gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac
      541 caaagaataa acggatccgc tgg
//
LOCUS       DAJTOW010000284          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTOW010000284 DAJTOW010000000
VERSION     DAJTOW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.289"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..539
                     /locus_tag="OO244_004169"
                     /pseudo
     CDS             1..539
                     /locus_tag="OO244_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT          118 a          166 c          145 g          110 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccggtg cggggagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtagcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc acatgcgcag caaaaaatcg ccagcggcga tttgccggca agcagttac
//
LOCUS       DAJTOW010000285          535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTOW010000285 DAJTOW010000000
VERSION     DAJTOW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.290"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>535)
                     /locus_tag="OO244_004170"
     CDS             complement(<1..>535)
                     /locus_tag="OO244_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5665772.1"
                     /translation="LPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNI
                     QENIIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFN
                     GPQFRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRR
                     EVSTQWTSFSTEENSRA"
BASE COUNT          161 a           84 c          135 g          155 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagag
//
LOCUS       DAJTOW010000286          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTOW010000286 DAJTOW010000000
VERSION     DAJTOW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.291"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(196..>526)
                     /locus_tag="OO244_004171"
     CDS             complement(196..>526)
                     /locus_tag="OO244_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5665773.1"
                     /translation="ITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAELFGG
                     EGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKAAFSK
                     QSIAKVD"
BASE COUNT          136 a          135 c           95 g          160 t
ORIGIN      
        1 accactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
       61 catctgacca ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc
      121 ggcggcgggt tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca
      181 tgagatatat gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg
      241 ttctgaacgt gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg
      301 tttcattaca acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc
      361 cagtaatgcc agctcctgat attcataacc ttccccacca aaaagttctg cttttaccgc
      421 ctgcgccgcc agccagatta attttttcag gcgttcctgt gttttctctg caattttcct
      481 agtgccagaa tgagccttaa actcttccca cgcccagtgg gttatg
//
LOCUS       DAJTOW010000287          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTOW010000287 DAJTOW010000000
VERSION     DAJTOW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.292"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>524)
                     /locus_tag="OO244_004172"
     CDS             complement(<1..>524)
                     /locus_tag="OO244_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5665774.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHS"
BASE COUNT          142 a          156 c          128 g           98 t
ORIGIN      
        1 cgagtgagtt gagaggaccg cgctcatatc gaaggcgatt tcctgaaagt ggttccacaa
       61 tccggcttca tgaaatgcgc gaatggcacc agccgccagc cctgcggcga cgaagagaat
      121 aaacaggctg gtccatttaa aaaatgcacc aagattgagg cgaataccgc cccagtagag
      181 caggaagccg agcaccacgg cagtagcaag accgagcatt gcacccagcg gcggccagat
      241 cccgacatct tgttgaaatg ccgccagcag gaaaaagacc gactccagcc cttcccttgc
      301 aacggcaaaa aagaccatca tcaccagcgc ccagccatga tgatttccac gctgcaatgc
      361 gctatcgact gcctgttcca gttgcacttt gacgttgcgc gacactttgc gcatccagaa
      421 aaccatccag gtaaggatca ccacggcgat caccgccacg ataccttcaa acagttcctg
      481 ctctttttgc ggaaattcgc cggtggtttc gttaatgaag atcc
//
LOCUS       DAJTOW010000288          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTOW010000288 DAJTOW010000000
VERSION     DAJTOW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.293"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>523
                     /locus_tag="OO244_004173"
     CDS             <1..>523
                     /locus_tag="OO244_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5665775.1"
                     /translation="MDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAV
                     GTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVEL
                     REIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTG
                     IDIDMHIDAASGG"
BASE COUNT          126 a          141 c          148 g          108 t
ORIGIN      
        1 tgatggattt gtcgatcaat aaaaactgga tcgacaaaga agaatatccg caatccgcag
       61 ccatcgacct gcgttgcgta aacatggttg ccgatctgtg gcatgcgcct gcgccgaaaa
      121 atggtcaggc cgttggcacc aacaccattg gttcttccga ggcctgtatg ctcggcggga
      181 tggcgatgaa atggcgttgg cgcaagcgta tggaagctgc aggcaaacca acggataaac
      241 caaacctggt gtgcggtccg gtgcaaatct gctggcataa attcgcccgc tactgggatg
      301 tggagctgcg tgagatccct atgcgccccg gtcagttgtt tatggacccg aaacgcatga
      361 ttgaagcctg cgacgaaaat accatcggcg tggtgccgac tttcggcgtg acctacaccg
      421 gtaactatga gttcccgcaa ccgctgcacg atgcactgga taaattccag gccgacaccg
      481 gtatcgacat cgacatgcac atcgacgccg ccagcggtgg ctt
//
LOCUS       DAJTOW010000289          517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTOW010000289 DAJTOW010000000
VERSION     DAJTOW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.294"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>517
                     /locus_tag="OO244_004174"
     CDS             <1..>517
                     /locus_tag="OO244_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5665776.1"
                     /translation="PAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKL
                     APRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRV
                     LAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGE
                     HPSRRGNKALKR"
BASE COUNT          100 a          174 c          142 g          101 t
ORIGIN      
        1 tccggcaccg gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct
       61 ccagcgatat ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca
      121 gctctgcaaa cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact
      181 ggccgaacaa accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct
      241 ggcactccag ctcatcacgc tgcgtaagca aagagacgag gtggcgcttg aggtagaaca
      301 gcgagttctt gctcaccctc tttacccggt cctgaccagt atgcccggag tcggtgtcag
      361 gaccgcagcc agactcctca ccgaggtcgc ctgccgcgcc ttcgcctctg ccgcacatct
      421 cgctgcttat gctggccttg cgccggtaac tcggcgatcc ggctcgtcaa tacgcggtga
      481 gcatccctcg cgacggggta ataaagctct caaacgg
//
LOCUS       DAJTOW010000290          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTOW010000290 DAJTOW010000000
VERSION     DAJTOW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.295"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>501)
                     /gene="arsB"
                     /locus_tag="OO244_004175"
     CDS             complement(<1..>501)
                     /gene="arsB"
                     /locus_tag="OO244_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5665777.1"
                     /translation="NYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFVLEPLGIPV
                     SAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLS
                     GVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYA
                     NVIGCD"
BASE COUNT          138 a          140 c          128 g           95 t
ORIGIN      
        1 aatcgcagcc aatcacgttg gcataaacca tcgcttcttt gataacgcca gatgccgtgc
       61 tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat attgttcata atagaagaga
      121 ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca caggccgtta tccgccagca
      181 cgttgagtac gccagaaaga tattccgtta atccggcatt gcgcaggcca taaaccacca
      241 gatacatgcc gagcgagaag atgacaatct gccagggggc accgcgcagg actttacccg
      301 tattaatcgc atgaccgcgt ttagcgacga caaataatat cagtgcgccc acagctgcaa
      361 tggcgctcac cggaatgccg agcggttcca ggacgaaaaa tcccaccagc agaagcagta
      421 aaacaaccca gccagttttg aacgtagcag gatctttgat cgcttctgcg ggagatttca
      481 gcagcgccat atcgtagttc t
//
LOCUS       DAJTOW010000291          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTOW010000291 DAJTOW010000000
VERSION     DAJTOW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.296"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            285..>488
                     /locus_tag="OO244_004176"
     CDS             285..>488
                     /locus_tag="OO244_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5665778.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT          136 a          111 c          102 g          139 t
ORIGIN      
        1 gacttagaag gtcgttgctc tatccaactg agctaagggc gcgttgatac cgcaatgcgg
       61 tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca atggcttttg atctggttgc
      121 tgaacaagtg aacgaccgcg tctgattttc tgatttattt cgctatagcg gcaaacaaac
      181 gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt catgtatcaa agtacaattt
      241 accgccctaa cggaaaattg tccgctccta tgagactggt aactatgaaa ccaacgtcgg
      301 tgatcattat ggatactcat cctatcatca gaatgtctat tgaagttctg ttgcaaaaaa
      361 acagtgaatt gcagattgtc ctgaaaacgg atgattaccg cataaccatc gattatctcc
      421 gaacccgtcc tgttgattta atcattatgg atatagactt gcccggaaca gacggtttta
      481 ccttcctg
//
LOCUS       DAJTOW010000292          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTOW010000292 DAJTOW010000000
VERSION     DAJTOW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.297"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>481)
                     /locus_tag="OO244_004177"
     CDS             complement(<1..>481)
                     /locus_tag="OO244_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5665779.1"
                     /translation="AENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTIN
                     DNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALA
                     ADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASV
                     "
BASE COUNT          112 a          112 c          124 g          133 t
ORIGIN      
        1 aaccgatgcg ttgtccgtcg cgcgagttaa tggtttacct gcggctaatg gcgaatcaga
       61 gtgtttgatt gagcctaagt aacgcgtaat taacgccacg agtttgtctt ctgcgacatt
      121 accgacaatg acaaacgtga tatccgctgg agatgaaaac aactggcgat cggcagccag
      181 cgcatctgcg gcagtaaact gtgcaatctg attttcttgc agtaatttcg tgcggtcatc
      241 agcatagcgc gtctcataca tcagctgggc gaatttctcc gctggacgct ggtcgagcgt
      301 tttcaacgcc tgaatttgag cattttgtag cgatgcccaa atattgtcgt taatcgtgct
      361 gtgggtgatt cgctggttaa tcaactggaa accaggttca gggttattag tccgcgcgct
      421 aacggagagc aacgtattca tgccactgac tttactgctc atggttaccg agttttccgc
      481 a
//
LOCUS       DAJTOW010000293          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTOW010000293 DAJTOW010000000
VERSION     DAJTOW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.298"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..219)
                     /locus_tag="OO244_004178"
     CDS             complement(<1..219)
                     /locus_tag="OO244_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5665780.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..>474)
                     /locus_tag="OO244_004179"
     CDS             complement(216..>474)
                     /locus_tag="OO244_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HCR5665781.1"
                     /translation="GNSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFP
                     LPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGAKS"
BASE COUNT          111 a          126 c          119 g          118 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccg
//
LOCUS       DAJTOW010000294          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTOW010000294 DAJTOW010000000
VERSION     DAJTOW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.299"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..413
                     /locus_tag="OO244_004180"
                     /pseudo
     CDS             1..413
                     /locus_tag="OO244_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          168 a           96 c           81 g          128 t
ORIGIN      
        1 aatccgaact tacatcaatc aggtattggc acagaaacaa acttcaaccc tgaatacaaa
       61 ggtgattcat ctaagccaat agttctatcg ctgactggtt taatggtgca actaataaaa
      121 ttgctaactt ccaagaatcg atgaataact tcgtaaataa cacacaatca actccatcag
      181 ctatcacagg tgacatggtt aatagcaatg ctcaattagc tatggctgta tcagagttag
      241 gaagaaaggt agacgcttta acagcagaaa ctaagaagcc tttatcagtt aatatcactg
      301 gtgaagcaac tgttagaccg gatgcaccat ctttctttag cttcaatcaa tcagctaatg
      361 accaattcac gcaggatatg ctcttaagtt caagctatcc agaggaagaa tgatttctta
      421 tatatccgtg tgctacgtga aatattctga acagatatac ccactgaaaa ata
//
LOCUS       DAJTOW010000295          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTOW010000295 DAJTOW010000000
VERSION     DAJTOW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.300"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            101..>464
                     /locus_tag="OO244_004181"
     CDS             101..>464
                     /locus_tag="OO244_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665782.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLP"
BASE COUNT          108 a           99 c          143 g          114 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccg
//
LOCUS       DAJTOW010000296          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTOW010000296 DAJTOW010000000
VERSION     DAJTOW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.301"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            261..>447
                     /locus_tag="OO244_004182"
     CDS             261..>447
                     /locus_tag="OO244_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCR5665783.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTP"
BASE COUNT          127 a          101 c           93 g          126 t
ORIGIN      
        1 agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat gccacttgta
       61 attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat gctcctttgc
      121 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
      181 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta
      241 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
      301 tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc cgcgctcgcg
      361 aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
      421 atcaggcgct ggcgctggat acacctg
//
LOCUS       DAJTOW010000297          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTOW010000297 DAJTOW010000000
VERSION     DAJTOW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.302"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>442)
                     /locus_tag="OO244_004183"
     CDS             complement(<1..>442)
                     /locus_tag="OO244_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5665784.1"
                     /translation="IDCMSYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFI
                     SMDVDDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFD
                     PEELYSAREFYQDTINAFYGKQQYLFNPPWESLADKFQFREAELT"
BASE COUNT          111 a          119 c           99 g          113 t
ORIGIN      
        1 gcgtcaactc tgcctcacgg aactggaatt tatctgccag agattcccac ggtggattaa
       61 acagatactg ctgcttgccg tagaaagcgt tgatagtgtc ctggtaaaac tctcgcgcac
      121 tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat gtactgccag atttcatcct
      181 cggtaacacc gttcatcagg cattcgttga tgtctttgta cggcagagta acaagacggc
      241 aacgatgttc accgagtcgg cttacgattt cccttgcggc ttcacgacca acatcatcaa
      301 cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt gtgatactca aactcaatcc
      361 actgttgctt agcgcctttc ccgccaccaa acggcacgga taacgccgag atgccgtatt
      421 gcgcatagct catacaatca at
//
LOCUS       DAJTOW010000298          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTOW010000298 DAJTOW010000000
VERSION     DAJTOW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.303"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(1..279)
                     /locus_tag="OO244_004184"
     CDS             complement(1..279)
                     /locus_tag="OO244_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665785.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           95 a          103 c          134 g          102 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tccc
//
LOCUS       DAJTOW010000299          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTOW010000299 DAJTOW010000000
VERSION     DAJTOW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.304"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            203..>433
                     /locus_tag="OO244_004185"
     CDS             203..>433
                     /locus_tag="OO244_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5665786.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          137 a          103 c           61 g          132 t
ORIGIN      
        1 cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta tatccgtgat
       61 gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt cagaaaacaa
      121 acttattttt attaaaatca attctttttc tttgaacatt caactgcgat atttgtaaaa
      181 acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat tccccgcata
      241 tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc aatacgcacg
      301 cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatggcagac gtactgttga
      361 gatccacaaa ctagatctct ccgactggca agcattcaat aagttagcaa cgaagtgtaa
      421 tgcttacgat gga
//
LOCUS       DAJTOW010000300          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTOW010000300 DAJTOW010000000
VERSION     DAJTOW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.305"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>421)
                     /locus_tag="OO244_004186"
     CDS             complement(<1..>421)
                     /locus_tag="OO244_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5665787.1"
                     /translation="APHKTGAGIATPTMTTAHNRAQAVFLCVKHSHIQIMVGRAGQPQ
                     GWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATILTLSHPDATIENGRAVT
                     TSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVT"
BASE COUNT          105 a          104 c          118 g           94 t
ORIGIN      
        1 gtgacgggct taaaattaag ccggttaaac tccggcgagc attcgagagt ttcgatcttc
       61 tgaataacgt ttttgtgcat tttccggaag aactccgcaa ccgcaacaga cgtagtgaca
      121 gcgcgaccat tttcgatggt tgcgtcaggg tgagaaaggg taaggatagt agccatgatg
      181 gcagcctcga tagtcagttt aaatgactca ccaccagagg tagcaatctc atgggtggtg
      241 agacgcacag ggttgctaca accggtgact accgaaaccg gccagccttg cggctgccct
      301 gcacgcccca ccataatctg aatgtggctg tgcttgacgc ataaaaaaac cgcctgagcg
      361 cggttatgcg cagtagtcat cgtcggggta gcaatcccgg cacccgtttt atgaggtgca
      421 g
//
LOCUS       DAJTOW010000301          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTOW010000301 DAJTOW010000000
VERSION     DAJTOW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.306"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            29..416
                     /locus_tag="OO244_004187"
                     /pseudo
     CDS             29..416
                     /locus_tag="OO244_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           94 a          110 c          108 g          104 t
ORIGIN      
        1 tgtatgatgg tgattttaag gtgcttgcgt ggcttccatt tccatcagat gtccttcctg
       61 ctccgctact gaaggcgtgg tgcgtaacgg caaaagcact gccggacatc agcgctatct
      121 ctgctctcat tgccgtaaaa catggcaact acagttcact tacaccgcct ctcagcccgg
      181 tacgcaccag aaaatcattg atatggccat gaatggcgtc ggatgtcgcg ccagtgcacg
      241 cattatgggc gttggcctca acacggtttt acgtcactta aaaaactcag gccgcagtcg
      301 gtaacctcgc gcatacaacc gggcagtgat gtgattgtct gcgctgaaat ggacgaacat
      361 tggggctacg tcggtgctaa atcacgtcag cgctggctgt tttacgcgta tgacag
//
LOCUS       DAJTOW010000302          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTOW010000302 DAJTOW010000000
VERSION     DAJTOW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.307"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>411
                     /gene="ipaH9.8"
                     /locus_tag="OO244_004188"
     CDS             <1..>411
                     /gene="ipaH9.8"
                     /locus_tag="OO244_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5665788.1"
                     /translation="TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDL
                     RTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYPQRVAD
                     RLKASGLSGDADAEREAGAQVMRETEQQIYRQLTD"
BASE COUNT          104 a           95 c          130 g           82 t
ORIGIN      
        1 actctccatt ttgtggatga gatagaagtc tacctggcct tccagaccat gctcgcagag
       61 aaacttcagc tctccactgc cgtgaaggaa atgcgtttct atggcgtgtc gggagtgaca
      121 gcaaatgacc tccgcactgc cgaagccatg gtcagaagcc gtgaagagaa tgaatttacg
      181 gactggttct ccctctgggg accatggcat gctgtactga agcgtacgga agctgaccgc
      241 tgggcgcagg cagaagagca gaaatatgag atgctggaga atgagtaccc tcagagggtg
      301 gctgaccggc tgaaagcatc aggtctgagc ggtgatgcgg atgcggagag ggaagccggt
      361 gcacaggtga tgcgtgagac tgaacagcag atttaccgtc agctgactga c
//
LOCUS       DAJTOW010000303          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTOW010000303 DAJTOW010000000
VERSION     DAJTOW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.308"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..147)
                     /locus_tag="OO244_004189"
     CDS             complement(<1..147)
                     /locus_tag="OO244_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5665789.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLI"
BASE COUNT          138 a           88 c           72 g          110 t
ORIGIN      
        1 gataagtaaa tctcgccacg gatcaaaacg acaatgccat aatttgcggc gcaactgctc
       61 aagacaaccg tgtatatcgc cagaaagcca gatatgtcgc cattgatgac ccgcaattct
      121 ctgataaacg ggcgcaggct gtttcatcaa tattttcctc ccgcgctaaa gatcacataa
      181 tcttaacaag aatgttaaaa aacgctggac tcagacagta gagtgtgtgt tatggttgac
      241 tataaagtca gcgaaggaaa tgcttctggc ttttaacaga taaaaagaga ccgaacatga
      301 ttcctgtttt cgtcaacaaa caataaaacc ctttaaaatt aatgcgttga ataaatttac
      361 gttcatcttt taatccctga cacgtaccat tgcatattaa tacattca
//
LOCUS       DAJTOW010000304          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTOW010000304 DAJTOW010000000
VERSION     DAJTOW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.309"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..105
                     /locus_tag="OO244_004190"
     CDS             <1..105
                     /locus_tag="OO244_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5665790.1"
                     /translation="DSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKR"
     gene            187..>398
                     /locus_tag="OO244_004191"
     CDS             187..>398
                     /locus_tag="OO244_004191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5665791.1"
                     /translation="MRYRNSRQVTGVVKNVTVSQSCGTWYISIQIESEVSTPAHPSAS
                     MVGLDAGVAKLATLSDGTVFEPVNSF"
BASE COUNT           98 a          104 c          102 g           94 t
ORIGIN      
        1 gattctccct cacagccatt gcaacagtca ctgaaagacc ttgagcgggc ttacaaaaac
       61 ttcttccgga agcgggctgc ttttccccga ttcaaaaagc ggtgacagaa tgatgcattc
      121 cgctacccgc agggcgttaa gctcgatcag gaaaacagcc gtatttttct gccgaaactg
      181 ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa tgtcactgtc
      241 agccagtcct gcggtacgtg gtacatcagt attcagatag aaagtgaagt atccactccg
      301 gctcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact cgccacgctg
      361 tcagatggca cagtctttga gcctgtaaac agttttca
//
LOCUS       DAJTOW010000305          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTOW010000305 DAJTOW010000000
VERSION     DAJTOW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.310"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>395
                     /locus_tag="OO244_004192"
     CDS             <1..>395
                     /locus_tag="OO244_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5665792.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEAART
                     LPHALRTLKLADEQIAELSMLCGFDDDLA"
BASE COUNT           93 a          120 c           94 g           88 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga
      241 cttacacgcc ggtgaagcta aaactgatgc tcgtgacgct gccatcattg ccgaagctgc
      301 ccgtaccctg cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact
      361 ctccatgctc tgcggcttcg atgatgatct tgccg
//
LOCUS       DAJTOW010000306          392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTOW010000306 DAJTOW010000000
VERSION     DAJTOW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.311"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(231..>392)
                     /locus_tag="OO244_004193"
     CDS             complement(231..>392)
                     /locus_tag="OO244_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5665793.1"
                     /translation="PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLI
                     YRTLLEKRD"
BASE COUNT           95 a          123 c          101 g           73 t
ORIGIN      
        1 cccccgggct ctggcgaaca aaatcgccgc tgcgcggtac cttcagctta tccccttcgg
       61 caaccgggtc gggcgcgagg taacgtcctg taagacgcgc cctcagccca catccatgtg
      121 ggctgccccg gccttcgggg aacgcttcag cgattttgac gccaccaata cccgtgcggt
      181 ttgtaatgtc gaagataaaa caaccagggc agataaaatg ccaatgctaa ttaatcacgc
      241 ttttccagca gagtccggta aatcagacca ccgataatgc cgccgacaat tggcaccacc
      301 cagaagaacc acagttgttc taatgcccag ccgccctgga agatagcaac cgcggtgctg
      361 cgcgccgggt taacagaagt gttagtcacc gg
//
LOCUS       DAJTOW010000307          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTOW010000307 DAJTOW010000000
VERSION     DAJTOW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.312"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            46..>390
                     /locus_tag="OO244_004194"
     CDS             46..>390
                     /locus_tag="OO244_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5665794.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKP"
BASE COUNT          113 a           84 c          108 g           85 t
ORIGIN      
        1 agattaagat gtaatataaa tgcatcttaa tgaggtggag gcagcatgtc cggcaagcgt
       61 atctctcgtg aaaaactgac gattaaaaaa atgatcgatc tttatcaagc gaaatgcccg
      121 caggcgtcag cggagccgga gcattacgag gcgttgtttg tttacgcgca aaagcggctg
      181 gataaatgtg tgttcggcga agagaaacca gcctgtaagc agtgtccggt gcactgttac
      241 cagcctgcaa agcgtgaaga gatgaaacag attatgcgtt gggccgggcc gagaatgctg
      301 tggcgtcatc cgatcttaac cgtgcgtcat ctcatcgatg acaagcgtcc ggtgccagaa
      361 ctgccggaaa aatatcgtcc gaaaaaacct
//
LOCUS       DAJTOW010000308          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTOW010000308 DAJTOW010000000
VERSION     DAJTOW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.313"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(172..>382)
                     /locus_tag="OO244_004195"
     CDS             complement(172..>382)
                     /locus_tag="OO244_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5665795.1"
                     /translation="EVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFT
                     GLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT           90 a          124 c           74 g           94 t
ORIGIN      
        1 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctcc
       61 atatgaacgg aaccgacgat taaactggat ggctctgatt gattcagggt atgaatggcg
      121 gttttttgct ccgtttccct caaaatggac gcaacttccc ctctgcggct ctcagccgca
      181 ccaccgcatc cgggccagcg gctcatgcat caggacctgc tctgccagac ggtagccccg
      241 cttcagcccc gtaaaacgca tctgactccc gcacagcacg cacttcagcg ggtcaacctt
      301 cagtaacctc tgatacatcc ctctccaggt gatttgcatc gccgtttttc tcactgtctc
      361 cgttatgatg tacaccactt ct
//
LOCUS       DAJTOW010000309          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTOW010000309 DAJTOW010000000
VERSION     DAJTOW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.314"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..358)
                     /locus_tag="OO244_004196"
     CDS             complement(<1..358)
                     /locus_tag="OO244_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5665796.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASN"
BASE COUNT          128 a          119 c           73 g           62 t
ORIGIN      
        1 cgttagaagc aaaaccgctg gctgccgcgt caaaacccgt tttaccactg gcaattaaat
       61 acagcagcgc cgctgcaaca ataccgccga caacctgggc aattacgtag ccaacgactt
      121 cttttgccgg aaaacgtccg ccagcccata aaccaatagt gaccgccggg ttaaaatgac
      181 caccagaaat atgaccaaca gcgaaggcca tcgtcagaac ggtcaaacca aatgccagcg
      241 ccacgccggc aaaaccaatg cctaattccg ggaagcctgc ggccagtaca gcactgccac
      301 agccaccaaa aacaagccag aaagtaccaa aacattcagc tgctaatttt ctgaacatat
      361 ccacctcaat taaaaattga cc
//
LOCUS       DAJTOW010000310          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTOW010000310 DAJTOW010000000
VERSION     DAJTOW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.315"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..340)
                     /locus_tag="OO244_004197"
     CDS             complement(<1..340)
                     /locus_tag="OO244_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCR5665797.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGG"
BASE COUNT           95 a           91 c          106 g           88 t
ORIGIN      
        1 ttccaccgta ggtagcgacg ttacggatct gcggtccagc aatgaacgtg gccgcagcac
       61 ataacgccgg gagatgacgt tgagttatag gatcttctat tagctgggta aatgtcgttg
      121 cagagccgat acgtagcgag ccatcttccg ccagcgtaat tccccgcagc tccgccagat
      181 tatgaatatc aacaatatgg cgataacggt cattgtgatg gtggagctga atcagtacgt
      241 cagtgccacc ggcgagcagt ttggcctgcg ggttgtcagc cagcaggttg atggcatcgg
      301 caagggttgc tgcgcgatgg taagaagcaa aatcaaacat gatgttatcc tcaaatcaat
      361 cctgccagat ggaactcttc
//
LOCUS       DAJTOW010000311          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTOW010000311 DAJTOW010000000
VERSION     DAJTOW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.316"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            21..375
                     /locus_tag="OO244_004198"
                     /pseudo
     CDS             21..375
                     /locus_tag="OO244_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          120 a           89 c          103 g           63 t
ORIGIN      
        1 agtaagttgg agtcattacc gaacaacaag aagcgtttat gacgcttgag caggagcagc
       61 aggttaaaac ccgtactgcc gaacagaatg cacgtattgc ggcttttgaa gctgaacgtc
      121 gtcgtgaagc agagcagacg cgaattctgg ctgaacgaca aattcaggaa acggagatcg
      181 agcgcgagca ggccgtccgc tcaagaaagg ttgaggctga acgtgaagtt cgtattaaag
      241 agatcgaaca gcagcaggtc accgaaatcg ccaaccagac gaaatcgatc gctattgccg
      301 ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa ccttgcactc gcagaagcag
      361 taagtgcgca acaaa
//
LOCUS       DAJTOW010000312          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTOW010000312 DAJTOW010000000
VERSION     DAJTOW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.317"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>371
                     /locus_tag="OO244_004199"
     CDS             <1..>371
                     /locus_tag="OO244_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5665798.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPWVC"
BASE COUNT          116 a           75 c           95 g           85 t
ORIGIN      
        1 ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac
       61 ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg
      121 gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac gatacttgat
      181 atgttcggtc ccccaaaaac aaatgccggg atccggacgg taacattact gcagccggct
      241 cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag cgaaatcact
      301 ttttatcatc gggagtacgg cagaactgaa aaacaaaaac tgcattttgt tttcatgccc
      361 tgggtgtgta a
//
LOCUS       DAJTOW010000313          369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTOW010000313 DAJTOW010000000
VERSION     DAJTOW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.318"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            1..>369
                     /locus_tag="OO244_004200"
     CDS             1..>369
                     /locus_tag="OO244_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter"
                     /protein_id="HCR5665799.1"
                     /translation="MIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSR
                     PLKLIRSPYIILSATYVIGQI"
BASE COUNT           79 a           88 c          100 g          102 t
ORIGIN      
        1 ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa
       61 cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt ctggttaggg
      121 ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga catttataat
      181 gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat tatggcggtg
      241 ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt gagcttgcta
      301 agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac ttacgtcatc
      361 ggccaaatc
//
LOCUS       DAJTOW010000314          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTOW010000314 DAJTOW010000000
VERSION     DAJTOW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.319"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            179..>365
                     /locus_tag="OO244_004201"
     CDS             179..>365
                     /locus_tag="OO244_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5665800.1"
                     /translation="MRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDNL
                     KKINGKNEKLLGGKTART"
BASE COUNT          127 a           73 c           91 g           74 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaa
//
LOCUS       DAJTOW010000315          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTOW010000315 DAJTOW010000000
VERSION     DAJTOW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.320"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
BASE COUNT          102 a           77 c           75 g          109 t
ORIGIN      
        1 gcaaacgccc ggcttgattc tccattcatt gcctcttcaa ggcgttcctt gaaacgcatt
       61 atttccgggg gcataaaaat gcgctctgca tttttgcttt ctttttcacc tgtcatgccc
      121 aatctctctg ttatataaag aattttacag aattctttga tttgcactgg ttaaaataaa
      181 aatgcgcata gcttttgaat tgagcatttt tctgcgatat cgtttttccc gatagcggta
      241 gccggtatca gttaccgaaa acgttaagag agtaaggatc gcagaatgtc agcaaaaaac
      301 caagactggc acgcagagca gataaaggct gctgttcgta tgaaggggaa gacactatct
      361 caa
//
LOCUS       DAJTOW010000316          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTOW010000316 DAJTOW010000000
VERSION     DAJTOW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.321"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>359
                     /gene="pgrR"
                     /locus_tag="OO244_004202"
     CDS             <1..>359
                     /gene="pgrR"
                     /locus_tag="OO244_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5665801.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDK"
BASE COUNT           99 a           64 c           91 g          105 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaatt
//
LOCUS       DAJTOW010000317          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTOW010000317 DAJTOW010000000
VERSION     DAJTOW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.322"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
BASE COUNT          128 a           52 c           54 g          123 t
ORIGIN      
        1 cagaatctat aaaggggtat agcgaaaaaa cattgcatta gaaaagtatg ttttcgaaca
       61 ttcaataaat aaatagagat atgcatcaca aatacaataa caaacaaaat gttgattttg
      121 tgatgaacat aacaaataca aagttgactt taaaaatatc aatttcatat tgttgttatt
      181 taaggtttta ttattttggt ttgtggggtt tttcttctcg cgtacccaat tgctttcatt
      241 aaaccgcacg agtattttat ttttaccgcc ggacggtacc ctaaaaataa aataaggata
      301 atttatgcgg cttagtgatt attttccaga atcatcaatc tcagttatac attcagc
//
LOCUS       DAJTOW010000318          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTOW010000318 DAJTOW010000000
VERSION     DAJTOW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.323"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            108..>357
                     /locus_tag="OO244_004203"
     CDS             108..>357
                     /locus_tag="OO244_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5665802.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQV"
BASE COUNT           95 a           74 c          108 g           80 t
ORIGIN      
        1 gcggggttgc ttatcgacga cgagcagttt gatgaaacca atattgtgcg cggactgcct
       61 gttcctggtg gtcggttggg gataaaaatc acagagctgg agtgtgcatg aataaccagt
      121 atttacagtt tgttcgtgag cagctcatta tcgccaccgc tgatttgagt ggggcgacaa
      181 aaggtcagct tgaagcctgg caggagaatg ccatgttcga tacagggcgt tacaggcgta
      241 aaaaaatccg gtaccgcgat gaggtgaccg gaaaaatgat aacgcgggat aatccaccaa
      301 tcccgggaaa acaatcactg gcgaaggggg cgtcaattcc tctggtcagt caggttg
//
LOCUS       DAJTOW010000319          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTOW010000319 DAJTOW010000000
VERSION     DAJTOW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.324"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..217)
                     /locus_tag="OO244_004204"
     CDS             complement(<1..217)
                     /locus_tag="OO244_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR5665803.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFV"
BASE COUNT          120 a           89 c           75 g           73 t
ORIGIN      
        1 tcacgaaggt gagatattta atggaaacca caaagattag cagccagaag atcagcgata
       61 aaaagccaaa cacggcatcg cgttcaacgc caaaaccaaa ctggccggac aaacattcac
      121 gaagtgtata taacgggctg gtaccaatat cgccgtagac aactccaatc gccgcgaggg
      181 taatcgcggg caatgattgc ttattatcag tgctcataga ctagtctttc gttgaaatat
      241 gaaatgtgtg cttagtccct tggcccacaa aaaagcgcac agtatgcacg attaacggca
      301 aaatcgtact cctaaatgcg gccacattaa cctggctcaa agaaaaagcc ccccctt
//
LOCUS       DAJTOW010000320          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTOW010000320 DAJTOW010000000
VERSION     DAJTOW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.325"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(170..319)
                     /locus_tag="OO244_004205"
                     /pseudo
     CDS             complement(170..319)
                     /locus_tag="OO244_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
BASE COUNT           96 a           91 c           72 g           94 t
ORIGIN      
        1 cctccatgaa tactcgcggc atcacgcaga acgacgcatg cgtaagacca aaccacagtt
       61 gcaggtcttt acgatattct tcatccatcg tctttacctt tgttgcaata aaaagccccg
      121 cgaatgcgag gctaaatcct ggtgtttgtg atgactggct cttatctaaa cgcagcccct
      181 taccgcgcgc caaatgctca actttaagca tcagcaatga gatgtttaat ctggattcac
      241 tccagaagtg atcaccaccc tgtctacaga gccagatgtg aaggatgatg agtaaaatta
      301 tctctatcat cgaaggcatt gcgtcctgat atattcctga agcgttctca gtg
//
LOCUS       DAJTOW010000321          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTOW010000321 DAJTOW010000000
VERSION     DAJTOW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.326"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(185..>346)
                     /locus_tag="OO244_004206"
     CDS             complement(185..>346)
                     /locus_tag="OO244_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665804.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          119 a           82 c           58 g           87 t
ORIGIN      
        1 tgcgagtgct gcaaactcga aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg
       61 atattcaaat taagtaaatg tcaaccgacg gggactatcc ccgtcataga cgttaacgcc
      121 ccactcctct ctgtcgctca agaggttaat gacgaatagc agagaacata atgtaaacaa
      181 ggcatcactc acgacacata aataacatct ttgtggtggt ttgattgatt tttccactcc
      241 gcttaccatc tacaccaaaa taacgatcat cacgacataa tttttcttta acatcgcaaa
      301 atacaccgtt agcgaatgta aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAJTOW010000322          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTOW010000322 DAJTOW010000000
VERSION     DAJTOW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.327"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>340)
                     /locus_tag="OO244_004207"
     CDS             complement(<1..>340)
                     /locus_tag="OO244_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5665805.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJTOW010000323          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTOW010000323 DAJTOW010000000
VERSION     DAJTOW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.328"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
BASE COUNT          110 a           82 c           77 g           70 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttatt
//
LOCUS       DAJTOW010000324          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTOW010000324 DAJTOW010000000
VERSION     DAJTOW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.329"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..>336)
                     /locus_tag="OO244_004208"
     CDS             complement(<1..>336)
                     /locus_tag="OO244_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5665806.1"
                     /translation="IALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLS
                     CLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAH
                     DMVVCVQQP"
BASE COUNT          114 a           82 c           72 g           68 t
ORIGIN      
        1 tcggttgttg cacacacacc accatgtcat gcgcaatgtt tgccagcatt atggagaaga
       61 aaactatcca gaaaatagat tgtgcttcaa gcgccataaa gaaaggaaat gcgctcaacg
      121 ttccgatcaa cgcgcctgtg atataaaccc tgcgccgacc aaaacgatcg gcaagccagg
      181 caaaacaggg aattgtcagg cagcttaatc cacctaccag caaaccaata ttaaggaaaa
      241 gttcgcgcgg taaccccata ttctgggttg aataattaag tgcaaaggca gtaacaatgt
      301 acatcgtcag caactcgcac agtcgtagtg caataa
//
LOCUS       DAJTOW010000325          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTOW010000325 DAJTOW010000000
VERSION     DAJTOW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.330"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..>323
                     /locus_tag="OO244_004209"
     CDS             <1..>323
                     /locus_tag="OO244_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5665807.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSN"
BASE COUNT           90 a           77 c           74 g           82 t
ORIGIN      
        1 aatggtaaag ttattgctcc agcttgtacc ctggtagctg cgacgaaaga ttcagtagtc
       61 acattgccaa atgtcagcgc caccaagttg caaactaatg gagcagtctc tggcgttaaa
      121 actgatgtac caattgcctt agaaggctgt gatgttactg taactaaaaa tgcgacgttt
      181 acctttagtg gtacggctga cggcgtgcaa cctacagcat ttgccaacca ggctaccacc
      241 gatgcagcga cgaatgttgc tctacaaatg tatctgccag atggttctac atcggtaact
      301 cccggtaccg aaacgtcgaa tat
//
LOCUS       DAJTOW010000326          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTOW010000326 DAJTOW010000000
VERSION     DAJTOW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.332"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(89..283)
                     /locus_tag="OO244_004210"
     CDS             complement(89..283)
                     /locus_tag="OO244_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5665808.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT           85 a           82 c           58 g           97 t
ORIGIN      
        1 tattttaatt cttttaagac tcttcaaaaa tgtcatcgta gcctttaaaa acgaatttta
       61 aaggcaccct aaaggcaggt caatgaagtt aacccgcctt cgcgtttaca ctctctgaga
      121 gaatactagc ttcaatctct tccaaaatct ctctgacttg ctcgtcgggc atatcaacga
      181 gcatcaacgc tgccaggcgt ttacggccct ctagttgcag tagctcttga gtttccttat
      241 ctaacaagcg tagcacagtc tcaacacctt tccttgcgag catgtttgta agggcttctc
      301 gttcctcact ggtaagaaac ga
//
LOCUS       DAJTOW010000327          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTOW010000327 DAJTOW010000000
VERSION     DAJTOW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.333"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..296
                     /locus_tag="OO244_004211"
     CDS             <1..296
                     /locus_tag="OO244_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR5665809.1"
                     /translation="VAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIA
                     EMVPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT           70 a           74 c           86 g           82 t
ORIGIN      
        1 gtgtggcagt aattatcgtg accggtattc cgttgattat cgccgataaa ttgattggcg
       61 gtggcgatgg cactgccgga attgccgctt ccagttccgc aggggccgcg gtagcgacac
      121 ctgtgctgat tgcagaaatg gtgcctgcgt ttaaaccgat ggctccggca gcaacttcgc
      181 tggtagcgac ggcggtcatt gtgacttcga ttctggtgcc aattcttacc tctatctggt
      241 cacgtaaagt caaagccaga gcagcgaaaa tcgaaatttt aggtacggtg aaataaacct
      301 gcttccttaa tc
//
LOCUS       DAJTOW010000328          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTOW010000328 DAJTOW010000000
VERSION     DAJTOW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.denovo.334"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
BASE COUNT          106 a           60 c           44 g           94 t
ORIGIN      
        1 caggaaatcg aaattattca aagaattaag actatgctcc ataagcacat cctccggtta
       61 tcgcatacct tttaaggcca tatgcttttt ctgatttcag tattccccaa tcagtgtagt
      121 gcctttaaac ataacgtgcg taaatatatt gtccccgatc acacttttta gtgaaaaata
      181 aaaaaccata ttaaaatact ttctcttcca gaacttctct aacatgtgtt attattaaca
      241 aagacaaatg taagcaggga aactattcca aggtaaatat tgcgtcacta aatggacatt
      301 ggag
//
LOCUS       DAJTOW010000329         5622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.guided.167, whole genome shotgun sequence.
ACCESSION   DAJTOW010000329 DAJTOW010000000
VERSION     DAJTOW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.guided.167"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..262
                     /locus_tag="OO244_004212"
     CDS             <1..262
                     /locus_tag="OO244_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR5665810.1"
                     /translation="SRGDYSRLVQPVRSEETLHSLAHGAGRKWGRTECKGRLAAKYTA
                     TQLSRTELGSRVICRDKQLIFEEAPQAYKSAESVVQCLGRPD"
     gene            324..938
                     /locus_tag="OO244_004213"
                     /pseudo
     CDS             324..938
                     /locus_tag="OO244_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(995..2452)
                     /gene="pepD"
                     /locus_tag="OO244_004214"
     CDS             complement(995..2452)
                     /gene="pepD"
                     /locus_tag="OO244_004214"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5665811.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2714..3172
                     /gene="gpt"
                     /locus_tag="OO244_004215"
     CDS             2714..3172
                     /gene="gpt"
                     /locus_tag="OO244_004215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5665812.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3264..4508
                     /gene="frsA"
                     /locus_tag="OO244_004216"
     CDS             3264..4508
                     /gene="frsA"
                     /locus_tag="OO244_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5665813.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4566..4967
                     /gene="crl"
                     /locus_tag="OO244_004217"
     CDS             4566..4967
                     /gene="crl"
                     /locus_tag="OO244_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5665814.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5006..>5622)
                     /locus_tag="OO244_004218"
     CDS             complement(5006..>5622)
                     /locus_tag="OO244_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5665815.1"
                     /translation="ASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGT
                     SLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYS
                     ETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYID
                     VGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         1359 a         1389 c         1483 g         1391 t
ORIGIN      
        1 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
       61 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      121 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
      181 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
      241 atgtctgggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
      301 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
      361 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
      421 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
      481 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
      541 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
      601 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
      661 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
      721 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
      781 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
      841 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
      901 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
      961 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1021 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1081 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1141 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     1201 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     1261 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     1321 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     1381 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     1441 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     1501 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     1561 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     1621 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     1681 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     1741 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     1801 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     1861 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     1921 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     1981 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2041 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2101 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     2161 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     2221 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     2281 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     2341 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     2401 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     2461 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     2521 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     2581 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     2641 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     2701 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     2761 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     2821 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     2881 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     2941 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3001 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3061 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3121 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     3181 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     3241 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     3301 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     3361 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     3421 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     3481 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     3541 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     3601 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     3661 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     3721 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     3781 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     3841 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     3901 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     3961 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4021 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4081 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4141 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     4201 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     4261 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     4321 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     4381 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     4441 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     4501 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     4561 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     4621 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     4681 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     4741 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     4801 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     4861 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     4921 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     4981 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5041 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5101 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     5161 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     5221 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     5281 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     5341 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     5401 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     5461 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     5521 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     5581 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gc
//
LOCUS       DAJTOW010000330         4435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.guided.185, whole genome shotgun sequence.
ACCESSION   DAJTOW010000330 DAJTOW010000000
VERSION     DAJTOW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.guided.185"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..1007)
                     /gene="intI1"
                     /locus_tag="OO244_004219"
     CDS             complement(<1..1007)
                     /gene="intI1"
                     /locus_tag="OO244_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5665816.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     S"
     gene            1164..1637
                     /locus_tag="OO244_004220"
     CDS             1164..1637
                     /locus_tag="OO244_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA15"
                     /protein_id="HCR5665817.1"
                     /translation="MKLSLMAAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSSDENVLVFPSIDEALNHLKTITDHVIVSGGGEI
                     YKSLIDKVDTLHISTIDIEPEGDVYFPEIPSSFRPVFSQDFVSNINYSYQIWQKG"
     gene            1838..2185
                     /locus_tag="OO244_004221"
     CDS             1838..2185
                     /locus_tag="OO244_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCR5665818.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2179..3018
                     /gene="sul1"
                     /locus_tag="OO244_004222"
     CDS             2179..3018
                     /gene="sul1"
                     /locus_tag="OO244_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCR5665819.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3146..3646
                     /locus_tag="OO244_004223"
     CDS             3146..3646
                     /locus_tag="OO244_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5665820.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            3967..4035
                     /locus_tag="OO244_004224"
                     /pseudo
     CDS             3967..4035
                     /locus_tag="OO244_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4153..>4435
                     /locus_tag="OO244_004225"
     CDS             4153..>4435
                     /locus_tag="OO244_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR5665821.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAV"
BASE COUNT          939 a         1237 c         1288 g          971 t
ORIGIN      
        1 tcactagtga ggggcggcag cgcatcaagc ggtgagcgca ctccggcacc gccaactttc
       61 agcacatgcg tgtaaatcat cgtcgtagag acgtcggaat ggccgagcag atcctgcacg
      121 gttcgaatgt cgtaaccgct gcggagcaag gccgtcgcga acgagtggcg gagggtgtgc
      181 ggtgtggcgg gcttcgtgat gcctgcttgt tctacggcac gtttgaaggc gcgctgaaag
      241 gtctggtcat acatgtgatg gcgacgcacg acaccgctcc gtggatcggt cgaatgcgtg
      301 tgctgcgcaa aaacccagaa ccacggccag gaatgcccgg cgcgcggata cttccgctca
      361 agggcgtcgg gaagcgcaac gccgctgcgg ccctcggcct ggtccttcag ccaccatgcc
      421 cgtgcacgcg acagctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc
      481 cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc
      541 ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg ttccatacag aagctgggcg
      601 aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc
      661 accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc
      721 gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag
      781 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
      841 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
      901 gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
      961 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1021 tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1081 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1141 aagttaaccc ctaaggaagt atcgtgaaac tatcactaat ggcagcaatt tcgaagaatg
     1201 gagttatcgg aaatggccca gatattccat ggagtgccaa aggggaacaa ttactcttca
     1261 aagcgattac ctataatcag tggcttttgg taggccgaaa gactttcgag tcaatggggg
     1321 ctttacccaa ccgaaaatat gccgttgtaa ctcgttcaag cttcacttcc agtgatgaga
     1381 atgtattggt atttccatct atcgatgaag cgctaaatca tctgaagacg ataacggatc
     1441 atgtgattgt gtctggtggt ggtgaaatat acaaaagcct gatcgataaa gttgatactt
     1501 tacatatttc aacaatcgac attgagccag aaggtgatgt ctattttcca gaaatcccca
     1561 gtagttttag gccagttttt agccaagact tcgtgtctaa cataaattat agttaccaaa
     1621 tctggcaaaa gggttaacaa gtggcagcaa ctgaccgcca aaagtgtcac ttgttttgcc
     1681 aaaaagccgg caaaacaagc gccaattttg tcggcagctg tgccaggcgt tagatgcact
     1741 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     1801 gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt
     1861 tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac
     1921 taagcttgcc ccttccgccg ttgtcataat cggttatggc atcgcatttt attttctttc
     1981 tctggttctg aaatccatcc ctgtcggtgt tgcttatgca gtctggtcgg gactcggcgt
     2041 cgtcataatt acagccattg cctggttgct tcatgggcaa aagcttgatg cgtggggctt
     2101 tgtaggtatg gggctcataa ttgctgcctt tttgctcgcc cgatccccat cgtggaagtc
     2161 gctgcggagg ccgacgccat ggtgacggtg ttcggcattc tgaatctcac cgaggactcc
     2221 ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc gatcgaaatg
     2281 ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg
     2341 cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc cctgtccgat
     2401 cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta tgcgctcaag
     2461 cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct ctatcccgat
     2521 attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga tggcatcgcc
     2581 acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg gttcttcgag
     2641 gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat cctcgatccg
     2701 gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct gtcgaacctt
     2761 caaaagctga agtcggcgtt ggggcttccg ctattggtct cggtgtcgcg gaaatccttc
     2821 ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct tgcggcggaa
     2881 cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg agatctgcga
     2941 agcgcaatca ccttctcgga aaccctcgcg aaatttcgca gtcgcgacgc cagagaccga
     3001 gggttagatc atgcctagca ttcaccttcc ggccgcccgc tagcggaccc tggtcaggtt
     3061 ccgcgaaggt gggcgcagac atgctgggct cgtcaggatc aaactgcact atgaggcggc
     3121 ggttcatacc gcgccagggg agcgaatgga cagcgaggag cctccgaacg ttcgggtcgc
     3181 ctgctcgggt gatatcgacg aggttgtgcg gctgatgcac gacgctgcgg cgtggatgtc
     3241 cgccaaggga acgcccgcct gggacgtcgc gcggatcgac cggacattcg cggagacctt
     3301 cgtcctgaga tccgagctcc tagtcgcgag ttgcagcgac ggcatcgtcg gctgttgcac
     3361 cttgtcggcc gaggatcccg agttctggcc cgacgccctc aagggggagg ccgcatatct
     3421 gcacaagctc gcggtgcgac ggacacatgc gggccggggt gtcagctccg cgctgatcga
     3481 ggcttgccgc catgccgcgc gaacgcaggg gtgcgccaag ctgcggctcg actgccaccc
     3541 gaacctgcgt ggcctatacg agcggctcgg attcacccac gtcgacactt tcaatcccgg
     3601 ctgggatcca accttcatcg cagaacgcct agaactcgaa atctaacgtc cgttcgggca
     3661 tcgaggtcca tgtcggggtg ggacgggccc gtggcttcaa gatcacttgc agtccgaccg
     3721 cgatgtcttg gttgcgcgag aggttgtcga tatcctccac ttccatcatc aaccctggat
     3781 aatgccgccg ccgtcatcgc cgccgacgcc cgtgccgggc ttttcgggcc tgtcaggctt
     3841 gctcggcctt cagcctgcct gggcgagatc tccggcggac ggattaacgg cggagcttcg
     3901 ccgcctttcg tgcgtgtgaa ggccgaagat agttctctca aaaacatccg tttatgagag
     3961 ataccaaatg tcattttcag aagacgactg caccagttga ttgggcgtaa tggctgttgt
     4021 gcagccagct cctgacagtt caatatcaga agtgatctgc accaatctcg actatgctca
     4081 atactcgtgt gggctctgtt gcaaaaatcg tgaagcttga gcatgcttgg cggagattgg
     4141 acggacggaa cgatgacgga tttcaagtgg cgccatttcc agggtgatgt gatcctgtgg
     4201 gcggtgcgct ggtattgtcg ctatccgatc agctatcgcg accttgagga aatgctggcg
     4261 gaacgcggca tttcggtcga ccatacgacg atctatcgct gggtccagtg ctacgccccg
     4321 gagatggaga agcggctgcg ctggttctgg cggcgtggct ttgatccgag ctggcgcctg
     4381 gatgaaacct acgtcaaggt gcggggcaag tggacctacc tgtaccgggc agtcg
//
LOCUS       DAJTOW010000331         2810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.guided.208, whole genome shotgun sequence.
ACCESSION   DAJTOW010000331 DAJTOW010000000
VERSION     DAJTOW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.guided.208"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            complement(<1..487)
                     /gene="tetR(A)"
                     /locus_tag="OO244_004226"
     CDS             complement(<1..487)
                     /gene="tetR(A)"
                     /locus_tag="OO244_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR5665822.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TV"
     gene            593..1792
                     /gene="tet(A)"
                     /locus_tag="OO244_004227"
     CDS             593..1792
                     /gene="tet(A)"
                     /locus_tag="OO244_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR5665823.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1824..2708)
                     /locus_tag="OO244_004228"
     CDS             complement(1824..2708)
                     /locus_tag="OO244_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCR5665824.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
BASE COUNT          531 a          880 c          854 g          545 t
ORIGIN      
        1 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
       61 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
      121 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
      181 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
      241 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
      301 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
      361 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
      421 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
      481 ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
      541 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
      601 caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
      661 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
      721 ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct
      781 gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc
      841 tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg
      901 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat
      961 cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg
     1021 gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt
     1081 cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga
     1141 gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt
     1201 ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca
     1261 acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg
     1321 ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca
     1381 ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg
     1441 aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc
     1501 gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat
     1561 gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct
     1621 caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat
     1681 aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc
     1741 ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa
     1801 acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga
     1861 gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt
     1921 tgaagcgcac tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg
     1981 ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa
     2041 aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt
     2101 cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct
     2161 ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg
     2221 gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca
     2281 ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata
     2341 gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg
     2401 accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc
     2461 gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc
     2521 atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc
     2581 aacgcccgca gcatcgcgac cgtcatcggt gagaagttcg gcaggtattg ggtggtgaca
     2641 atgtaggtgc tgccccaaat ggcaggtgct atcgctgtga acaataaatc gggcgtgcgt
     2701 aatgacatgc gattacaaga cctccgctac ggcgatgggt ttttgagagt tgcaggcgcc
     2761 gcgatgaccg caccctgact tggactggcc ctgcctcgca ccaatccacc
//
LOCUS       DAJTOW010000332         1742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.guided.232, whole genome shotgun sequence.
ACCESSION   DAJTOW010000332 DAJTOW010000000
VERSION     DAJTOW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.guided.232"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            <1..176
                     /locus_tag="OO244_004229"
     CDS             <1..176
                     /locus_tag="OO244_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HCR5665825.1"
                     /translation="IGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAA
                     ILTVVTLIGVVTI"
     gene            239..1372
                     /locus_tag="OO244_004230"
     CDS             239..1372
                     /locus_tag="OO244_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5665826.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1461..>1742
                     /gene="blc"
                     /locus_tag="OO244_004231"
     CDS             1461..>1742
                     /gene="blc"
                     /locus_tag="OO244_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5665827.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAY"
BASE COUNT          436 a          447 c          434 g          425 t
ORIGIN      
        1 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
       61 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
      121 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
      181 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
      241 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
      301 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
      361 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
      421 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
      481 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
      541 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
      601 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
      661 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
      721 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
      781 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
      841 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
      901 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
      961 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     1021 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     1081 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     1141 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     1201 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     1261 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     1321 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     1381 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     1441 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     1501 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     1561 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     1621 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     1681 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     1741 ac
//
LOCUS       DAJTOW010000333         1308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103315
            SAMEA8899872-rid15819803.guided.244, whole genome shotgun sequence.
ACCESSION   DAJTOW010000333 DAJTOW010000000
VERSION     DAJTOW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899872
            Sequence Read Archive: ERR6005278
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478192.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,233
            CDSs (total)                      :: 4,161
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 152 of 326
            Pseudo Genes (incomplete)         :: 57 of 326
            Pseudo Genes (internal stop)      :: 143 of 326
            Pseudo Genes (multiple problems)  :: 26 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899872-rid15819803.guided.244"
                     /serovar="S. flexneri X"
                     /isolate="103315"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-05-06"
     gene            25..840
                     /gene="sul2"
                     /locus_tag="OO244_004232"
     CDS             25..840
                     /gene="sul2"
                     /locus_tag="OO244_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5665828.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            927..1229
                     /locus_tag="OO244_004233"
     CDS             927..1229
                     /locus_tag="OO244_004233"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5665829.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
BASE COUNT          250 a          390 c          377 g          291 t
ORIGIN      
        1 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
       61 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
      121 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
      181 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
      241 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
      301 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
      361 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
      421 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
      481 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
      541 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
      601 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
      661 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
      721 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
      781 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
      841 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
      901 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
      961 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     1021 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     1081 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     1141 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     1201 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     1261 cggggagccc gcagaattcg gaaaaaatcg tacgctaagg ttttccgg
//
